Chromomycin A3: Glycosidic antibiotic from Streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.Chromomycins: A complex of several closely related glycosidic antibiotics from Streptomyces griseus. The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. It is also used as an antineoplastic agent, especially for solid tumors.Olivomycins: A mixture of several closely related glycosidic antibiotics obtained from Actinomyces (or Streptomyces) olivoreticuli. They are used as fluorescent dyes that bind to DNA and prevent both RNA and protein synthesis and are also used as antineoplastic agents.Plicamycin: A tricyclic pentaglycosidic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA. It is used as a fluorescent dye and as an antineoplastic agent, especially in bone and testicular tumors. Plicamycin is also used to reduce hypercalcemia, especially that due to malignancies.Streptomyces griseus: An actinomycete from which the antibiotics STREPTOMYCIN, grisein, and CANDICIDIN are obtained.Phialophora: A mitosporic fungal genus. Phialophora verrucosa is a cause of chromomycosis (CHROMOBLASTOMYCOSIS). Ophiobolus is the teleomorph of Phialophora.Distamycins: Oligopeptide antibiotics from Streptomyces distallicus. Their binding to DNA inhibits synthesis of nucleic acids.Protamines: A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)Antibiotics, Antineoplastic: Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Lanthanoid Series Elements: Elements of the lanthanoid series including atomic number 57 (LANTHANUM) through atomic number 71 (LUTETIUM).Nuclear Magnetic Resonance, Biomolecular: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.Cobalt: A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Protein Footprinting: A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.DNA Footprinting: A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Deoxyribonuclease I: An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.Grasshoppers: Plant-eating orthopterans having hindlegs adapted for jumping. There are two main families: Acrididae and Romaleidae. Some of the more common genera are: Melanoplus, the most common grasshopper; Conocephalus, the eastern meadow grasshopper; and Pterophylla, the true katydid.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Chromosomes: In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Circular Dichroism: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).G-Quadruplexes: Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)Oligohymenophorea: A class of ciliate protozoa. Characteristics include the presence of a well developed oral apparatus and oral cilia being clearly distinct from somatic cilia.Wetlands: Environments or habitats at the interface between truly terrestrial ecosystems and truly aquatic systems making them different from each yet highly dependent on both. Adaptations to low soil oxygen characterize many wetland species.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Fisheries: Places for cultivation and harvesting of fish, particularly in sea waters. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cytogenetic Analysis: Examination of CHROMOSOMES to diagnose, classify, screen for, or manage genetic diseases and abnormalities. Following preparation of the sample, KARYOTYPING is performed and/or the specific chromosomes are analyzed.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.Karyotyping: Mapping of the KARYOTYPE of a cell.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.National Library of Medicine (U.S.): An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Newspapers: Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Chromatin: The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.Infertility, Male: The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.Semen Analysis: The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.Ibuprofen: A nonsteroidal anti-inflammatory agent with analgesic properties used in the therapy of rheumatism and arthritis.Sperm Count: A count of SPERM in the ejaculum, expressed as number per milliliter.Sperm Motility: Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.Semen: The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.

Chromomycin A3 staining as a useful tool for evaluation of male fertility. (1/49)

PURPOSE: Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate. METHODS: Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA3 positivity is expressed as the percentage in 200 spermatozoa. RESULTS: Percentage of CMA3 positivity showed not only a negative correlation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover, percentage of CMA3-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA3 positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA3-positive semen samples in comparison with low CMA3-positive semen samples. CONCLUSION: There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.  (+info)

The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. (2/49)

Human semen is heterogeneous in quality, not only between males but also within a single ejaculate. Differences in quality are evident, both when examining the classical parameters of sperm number, motility and morphology and in the integrity of the sperm nucleus. The aim of this study was to determine the efficiency of the PureSperm((R)), Percoll((R)) and swim-up preparation techniques to eliminate spermatozoa with nuclear anomalies. Semen samples were collected, washed and one part of the semen spread on a slide, the remainder was prepared using the swim-up, PureSperm((R)) or Percoll((R)) techniques. Spermatozoa from different fractions were fixed on slides and assessed. Sperm samples (n) from different men were stained using the chromomycin A(3) (CMA(3)) fluorochrome, which indirectly demonstrates a decreased presence of protamine (n = 31 for swim-up; n = 45 for PureSperm((R)); n = 39 for Percoll((R))). Spermatozoa prepared using PureSperm((R)) (n = 35) and Percoll((R)) (n = 37) were also examined for the presence of endogenous DNA nicks. Good quality spermatozoa should not possess DNA nicks and not stain (i.e. fluoresce) with CMA(3). When prepared using the swim-up technique the spermatozoa recovered showed no significant improvement with the CMA(3) staining. When spermatozoa were prepared using the PureSperm((R)) and Percoll((R)) techniques, a significant (P < 0.001) decrease in both CMA(3) positivity and DNA strand breakage was observed. These results indicate that both the PureSperm((R)) and Percoll((R)) techniques can enrich the sperm population by separating out those with nicked DNA and with poorly condensed chromatin.  (+info)

Evaluation of complexation of metal-mediated DNA-binding drugs to oligonucleotides via electrospray ionization mass spectrometry. (3/49)

The interactions of self-complementary oligonucleotides with a group of metal-mediated DNA-binding drugs, including chromomycin A(3), mithramycin and the novel compound UK-1, were examined via electrospray ionization quadrupole ion trap mass spectrometry. Both chromomycin and mithramycin were shown to bind preferentially to GC-rich oligonucleotide duplexes in a 2:1 drug:metal ratio, while UK-1 was shown to bind in a 1:1 drug:metal stoichiometric ratio without a strong sequence preference. These trends were observed in the presence of Co(2+), Ni(2+) and Zn(2+), with the exception that chromomycin-Zn(2+) complexes were not readily observed. The binding stoichiometries as well as the sequence specificities are in agreement with literature reports for solution studies. Binding selectivities and stabilities of the complexes were also probed using electrospray ionization mass spectrometry. Both of the GC-rich oligomers 5'-GCGCGC-3' and 5'-GCGCATGCGC-3' exhibited a binding preference for chromomycin over mithramycin in the presence of Co(2+) and Ni(2+). Energy-variable collisionally activated dissociation of the complexes was employed to determine the stabilities of the complexes. The relative metal-dependent binding energies were Ni(2+) > Zn(2+) > Co(2+) for UK-1-oligomer complexes and Ni(2+) > Co(2+) for both mithramycin and chromomycin complexes.  (+info)

Homolog pairing and two kinds of bouquets in the meiotic prophase of rye, Secale cereale. (4/49)

Chromosome configurations and structures during meiotic prophase were investigated by staining large repeated DNA sequences localized in the subtelomeric regions of all the chromosomes in rye, Secale cereale, in order to clarify when and how homolog pairing and bouquet formation occur. The changes of the spatial locations of chromosomes in the nucleus were investigated by the use of laser confocal microscopy, together with the surface-spreading method of silver nitrate staining to detect the formation of the synaptonemal complex. Homolog pairing in which homologs of four chromatids of a pair of homologs were coaligned in parallel but remained distinctly separate was microscopically detected for the first time in the present study. Homolog pairing showed the following characteristics: (1) it occurred at the leptotene-zygotene transition stage, prior to the formation of nodules and the synaptonemal complex; (2) the chromatin structure of chromosomes was in a state of decondensation; (3) it required no telomere clustering. These data suggest that homolog pairing represents a structure that indicates incipient recombination. After the homolog pairing stage, two kinds of bouquet configuration were found in zygotene. The commonly observed type was a loose bouquet, in which the subtelomeric regions were loosely aggregated. The other type was a definite bouquet, in which almost all the subtelomeric regions were conjugated, but this type was observed only in a limited number of the meiotic prophase cells of some individuals. It was concluded that the former represents the configuration of homologous recombination and the latter that of ectopic recombination.  (+info)

Crystal structure of the [Mg2+-(chromomycin A3)2]-d(TTGGCCAA)2 complex reveals GGCC binding specificity of the drug dimer chelated by a metal ion. (5/49)

The anticancer antibiotic chromomycin A3 (Chro) is a DNA minor groove binding drug belonging to the aureolic family. Chro likely exerts its activity by interfering with replication and transcription. Chro forms a dimer, mediated by a divalent metal ion, which binds to G/C-rich DNA. Herein we report the first crystal structure of Chro bound to d(TTG GCCAA)2 DNA duplex solved by multiwavelength anomalous diffraction (MAD) based on the chelated Co3+ ion. The structure of the Mg2+ complex was subsequently refined at 2.15 A resolution, which revealed two complexes of metal-coordinated dimers of Chro bound to the octamer DNA duplex in the asymmetric unit. The metal ion is octahedrally coordinated to the O1 and O9 oxygen atoms of the chromophore (CPH), and two water molecules act as the fifth and sixth ligands. The two coordinated water molecules are hydrogen bonded to O2 atoms of C5 and C13 bases. The Chro dimer binds at and significantly widens the minor groove of the GGCC sequence. The long axis of each chromophore lies along and stacks over the sugar-phosphate backbone with the two attached saccharide moieties (rings A/B and C/D/E) wrapping across the minor groove. DNA is kinked by 30 degrees and 36 degrees in the two complexes, respectively. Six G-specific hydrogen bonds between Chro and DNA provide the GGCC sequence specificity. Interestingly, DNA in concert with Chro appears to act as an effective template to catalyze the deamination of Co(NH3)6(3+), as shown by circular dichroism and crystal structure data. Our results present useful structural information for designing new anticancer drug derivatives in the future.  (+info)

Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (6/49)

The biosynthetic gene cluster of the aureolic acid type antitumor drug chromomycin A3 from S. griseus subsp. griseus has been identified and characterized. It spans 43 kb and contains 36 genes involved in polyketide biosynthesis and modification, deoxysugar biosynthesis and sugar transfer, pathway regulation and resistance. The organization of the cluster clearly differs from that of the closely related mithramycin. Involvement of the cluster in chromomycin A3 biosynthesis was demonstrated by disrupting the cmmWI gene encoding a polyketide reductase involved in side chain reduction. Three novel chromomycin derivatives were obtained, named chromomycin SK, chromomycin SA, and chromomycin SDK, which show antitumor activity and differ with respect to their 3-side chains. A pathway for the biosynthesis of chromomycin A3 and its deoxysugars is proposed.  (+info)

Human cervical mucus can act in vitro as a selective barrier against spermatozoa carrying fragmented DNA and chromatin structural abnormalities. (7/49)

PURPOSE: We have carried out experiments to determine if human cervical mucus can act as an in vitro selective barrier against spermatozoa morphologically normal that carry genetic structural abnormalities. METHODS: Sperm chromatin abnormalities have been evaluated by Chromomycin A3 and "endogenous" nick translation. RESULTS: The data obtained have shown that spermatozoa possessing higher levels of DNA protamination are more proficient in crossing the cervical mucus barrier. Moreover, the levels of positivity to endogenous nick translation treatment was practically zero in such spermatozoa. CONCLUSIONS: We suggest that sperm penetration of cervical mucus could be used to select sperm preparations free of fragmented DNA or chromatin structural abnormalities for assisted reproduction.  (+info)

Cytogenetic studies in three Pimelodella meeki populations (Pisces, Pimelodidae) from Tibagi River basin (Brazil). (8/49)

We analyzed cytogenetically specimens of Pimelodella meeki from Tibagi River at Limoeiro (LM) and from two tributaries, Couro do Boi (CB) and Gabriel da Cunha (GC) Rivers. All specimens presented 2n=46 chromosomes, which were the karyotypes composed by 15 pairs metacentric (M) + 6 pairs submetacentric (SM) + 2 pairs subtelocentric (ST). In specimens of GC, CB, and LM, the results of analyses of the nucleolus organizer regions (NORs), done by means of AgNO3 and CMA3 staining, showed that they are identical, located in terminal position on the short arm of a SM chromosome pair, and they were observed to be a size heteromorphism in some metaphase plates. FISH with 18S rDNA probe yielded evidence for these regions but not for the size variation, indicating that they are not due to a greater number of NOR cistrons in one of the homologue chromosomes. An interesting characteristic of these regions is that they could appear divided in blocks, as evidenced by all the techniques. This work makes clear the necessity for more deeply systematic studies, because of the confused taxonomic situation of the genus Pimelodella.  (+info)

TY - JOUR. T1 - Quantitative footprinting analysis of the chromomycin A3-DNA interaction. AU - Stankus, Allison. AU - Goodisman, Jerry. AU - Dabrowiak, James C.. PY - 1992. Y1 - 1992. N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5′-TGGCCA-3′, 3′-ACCGGT-5′ in the 18-mer with a binding constant of (2.7 ± 1.4) × 107 M-1. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is ∼ 105 M-1. Since it has been suggested that CHR binding alters DNA to the A configuration, quantitative footprinting studies using dimethyl sulfate, which alkylates at N-7 of guanine in the major groove, ...
15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004 ...
Various approaches are used to study the chromosomal makeup of cells. Traditional cytogenetic methods are based on the analysis of mitotic cells fixed onto slides to analyze their chromosomal composition (karyotype) by microscopy. This approach can be combined with FISH to detect specific sequences on morphologically distinct individual chromosomes. Disadvantages of this type of microscopic analysis are the amount of time and labor required to acquire and analyze typically less than a hundred cells. As a result, the statistical power of this type of analysis is limited. An alternative to traditional cytogenetic methods is flow karyotyping (1,2) a method to analyze chromosomes in suspension by flow cytometry. For bivariate flow karyotyping, the DNA composition of specific chromosomes in suspension is measured based on the DNA-specific dyes Hoechst 33258 and chromomycin A3 (3,4). In our protocol, we combine flow karyotyping and FISH to analyze repetitive DNA in individual chromosomes by flow ...
We have separated the nonhistone chro moso mal proteins of pea chro matin fro m other chro moso mal constituents and have studied so me of the biological functions of these proteins. After dissociatio
1EKH: A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance.
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T02360 (aalt,achr,acta,actc,amyb,amyc,asw,cmos,cthd,cyl,dfn,ehl,fek,fva,hta,kak,kmx,kpnk,lei,lfb,lsh,lys,mcol,msub,mtab,noe,oor,paru,phs,pje,png,ptd,rpln,sclo,scou,seny,sera,sfz,slb,slw,snl,sphc,sphy,srub,taj : calculation not yet completed ...
T01155 (aof,chro,cmax,cmos,dzi,eml,fpd,goc,hae,jre,kpd,lpg,lrn,mhos,mste,msyr,nob,oeu,oor,paro,pkb,pprf,psor,pvs,pzh,salj,slim,spir,tmar : calculation not yet completed ...
slide scanner - Lab work is more often than not left in Petri dishes and on slides, so the Ultra HD Scientific Slide Scanner is designed to help move samples to th...
1-Place a drop of nigrosin on slide, 2-Mix a loopful of broth culture with the nigrosin (no extra water is needed when the culture is taken from solid media as nigrosin contains a lot of water already), 3-Use a second slide to sweep the mixture across the first slide, making a color gradient, 4-Let the smear air dry ...
Today you are going to learn about how the digestion system functions. Read the explanation on slide 2 and answer the questions. You can also do extra research by looking at the different websites in slides 3-5 and completing the differenet activities. Record any interesting information on your white board.
You have 2 PDB codes: One that you found by searching, and one that was given to you by Imada-san and the TAs. If your found PDB code does not have ligand, give the following information on Slide 1 for two PDB codes: your found code, and your given code: ...
Streptomyces griseus subsp. griseus bacteriophage 22653 ATCC ® 11984-B1™ Designation: 22653 TypeStrain=False Application:
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Plicamycin (INN, also known as mithramycin; trade name Mithracin) is an antineoplastic antibiotic produced by Streptomyces plicatus. It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently reported in different places all with the same chromomycin core, but with different stereochemistry in the glycoside chain, a 1999 study has re-investigated the compound and proposed a revised structure. Plicamycin has been used in the treatment of testicular cancer, Pagets disease of bone, and, rarely, the management of hypercalcemia. Plicamycin has been tested in chronic myeloid leukemia. Plicamycin is currently used in multiple areas of research, including cancer cell apoptosis and as a metastasis inhibitor. One elucidated pathway shows it interacts by cross-binding chromatin GC-rich promoter motifs, thereby inhibiting gene transcription. "Mithramycin A". Fermentek. Wohlert, S. E.; Künzel, E.; Machinek, R.; Méndez, C.; Salas, J. A.; ...
Chro plus supplement - Drugs - Healthline. Revitol provides discount natural health and beauty products manufacturer direct to our customers. Find your favorite health supplements and natural beauty products here.
Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
01/31/05 20:41 PM >>> I need some help!! We have been experiencing some yeast and bacteria showing up on some of our special stains. It started about 2 weeks ago and seems to be sporadic (one day...no problems, the next day, several stains are affected). The yeast and bacteria (rods) are clumped together all over the slide, on the tissue sections, around the sections and on the slide in areas where there is no tissue. The stains we have seen the contamination are the Gram, PAS/D, PAS and GMS. All of these stains are automated except the Gram, so I do not think the special stainer is the problem. Here is what I have eliminated as problem areas: -Processor solutions and paraffin (we cut and stained a block from another institute and had contamination) -Waterbaths, slide drying boards, water containers (used to fill waterbathes), stainer containers, ice pans (all of the mentioned have been thoroughly bleached) -Tap water and DI water (all water supplies have been tested by sending a sample to ...
Ohio States senior class experienced a little bit of everything. The final two years included 24 wins, but two crushing losses.
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Ions as a source of reactants: Ions get neutralized when they reach the surface, they become an additional source of reactive species.. The synergy between ion bombardment and chemical etching was first shown by Coburn and Winters in the classical experiment shown on slide 2.. Besides enhancing the chemical etch, ions also play a major role in removing non-volatile by-products or etch products that require an activation energy to desorp from the surface. The removal of by-products and their redeposition onto the feature sidewall is the fundamental reason why plasma etching can obtain anisotropic profiles (slide 3). Factors that influence the anisotropy are (slide 4 ...
Disclosed is an automated staining apparatus including an arm moveable in three dimensions, and a hollow tip head located on the arm including integral reagent tip head, wash tip and blow tip for selectively dispensing gas and liquid onto microscope slides. Also disclosed are various sub-components of the apparatus that are specifically adapted to the processing of specimens on slides.
... is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
Cancer can often be detected in the arrangement of cells in a tissue sample. Once a sample tissue is taken from the patient, it is sent to the histotechnician (HT), who prepares the tiny sections of body tissues for microscopic examination by a pathologist. Working closely with the pathologist, the histologic technician processes tissue biopsies removed during surgery. The tissue is cut into very thin slices, mounted on slides and stained with special dyes to make the cell details visible under the microscope. By examining the section of tissue, the pathologist and the surgeon can learn if disease is present, or if it has spread, and decide the best course of treatment for the patient. The histotechnologist (HTL) has advanced training in how and why specimens are collected and processed for testing. That expertise qualifies the histotechnologist to manage even unexpected situations in the laboratory, such as solving technical or instrument problems, understanding the underlying health and ...
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Even relatively minor errors in chromatin remodeling during spermiogenesis are associated with sperm DNA damage and infertility, yet little is known about the etiology. Mice with severe NPYq deletions are infertile due to severe sperm differentiation defects (Ward and Burgoyne, 2006; Yamauchi et al., 2009). We have recently observed that sperm from these mice presented abnormal chromatin packaging and DNA damage. Moreover, when these sperm were injected into the oocytes, a significant increase of oocyte arrest at pronuclei stage and of chromosome aberrations in the fertilized eggs were noted (Yamauchi et al., 2010). Here we provide evidence that the deficiency of NPYq encoded gene Sly is associated with sperm DNA damage and poor sperm chromatin condensation, and propose that SLY plays a role in spermatid-specific chromatin remodeling.. How can Sly/SLY be involved in sperm DNA damage phenotype? SLY protein has been shown to control the postmeiotic expression of ,100 genes, the majority of which ...
Euphorbia Linnaeus, 1753 (Euphorbiaceae) is one of the most diverse and complex genera among the angiosperms, showing a huge diversity in morphologic traits and ecologic patterns. In order to improve the knowledge of the karyotype organization of Euphorbia hirta (2n = 18) and E. hyssopifolia (2n = 12), cytogenetic studies were performed by means of conventional staining with Giemsa, genome size estimations with flow cytometry, heterochromatin differentiation with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and Giemsa C-banding, fluorescent in situ hybridization (FISH) with 45S and 5S rDNA probes, and impregnation with silver nitrate (AgNO3). Our results revealed small metacentric chromosomes, CMA+/DAPI0 heterochromatin in the pericentromeric regions of all chromosomes and CMA+/DAPI− in the distal part of chromosome arms carriers of nucleolar organizing regions (NORs). The DNA content measurements revealed small genomes for both species: E. hirta with 2C = 0.77 pg and E. hyssopifolia
ID B1VKL9_STRGG Unreviewed; 119 AA. AC B1VKL9; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 18-JUL-2018, entry version 26. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG16864.1}; GN OrderedLocusNames=SGR_35t {ECO:0000313,EMBL:BAG16864.1}, SGR_7104t GN {ECO:0000313,EMBL:BAG23931.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}, and RC NBRC 13350 {ECO:0000313,EMBL:BAG16864.1}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT "Genome sequence of the ...
Eukaryotic DNA is packaged in chromatin, whose repeating subunit, the nucleosome, consists of an octamer of histone proteins wrapped by about 147bp of DNA. This packaging affects the accessibility of DNA and hence any process that occurs on DNA, such as replication, repair, and transcription. An early observation from genome-wide nucleosome mapping in yeast was that genes had a surprisingly characteristic structure, which has motivated studies to understand what determines this architecture. Both sequence and trans acting factors are known to influence chromatin packaging, but the relative contributions of cis and trans determinants of nucleosome positioning is debated. Here we present data using genetic approaches to examine the contributions of cis and trans acting factors on nucleosome positioning in budding yeast. We developed the use of yeast artificial chromosomes to exploit quantitative differences in the chromatin structures of different yeast species. This allows us to place approximately 150kb
Hydrocortisone reveals its action by activation of transcription of specific genes that is achieved by binding of hormones nuclear receptors (NRs) to cognate sites on DNA and recruiting coactivators of basal transcription machinery. NRs are able to overcome restricting conformation of chromatin by altering chromatin-remodeling machinery (chromatin-remodeling protein complexes, histone and nonhistone modifying enzymes). However, a possibility arise that genomic DNA accessibility to NRs as well as chromatin packaging pattern in nuclei implicates complex processes which involve regulation of DNA modifying enzymes activities.. Whether hydrocortisone induced decondensation of chromatin alter DNA accessibility in nuclei upon exogenously applied DNAse I and cause eventual changes in endogenous Ca-Mg endonuclease-depended fragmentation become a focus of our research.. Experiments were conducted in vivo. Hydrocortisone 5 mg/100g body wt was administrated by intraperitonial injection. Hormone treated and ...
TY - JOUR. T1 - pRb2/p130 and p107 control cell growth by multiple strategies and in association with different compartments within the nucleus. AU - Zini, Nicoletta. AU - Trimarchi, Carmela. AU - Claudio, Pier Paolo. AU - Stiegler, Peter. AU - Marinelli, Fiorenzo. AU - Maltarello, Maria Cristina. AU - La Sala, Dario. AU - De Falco, Giulia. AU - Russo, Giuseppe. AU - Ammirati, Giuseppe. AU - Maraldi, Nadir Mario. AU - Giordano, Antonio. AU - Cinti, Caterina. PY - 2001. Y1 - 2001. N2 - It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the ...
The repair of DNA damage in highly compact, transcriptionally silent heterochromatin requires that repair and chromatin packaging machineries be tightly coupled and regulated. KAP1 is a heterochromatin protein and co-repressor which binds to HP1 during gene silencing, but is also robustly phosphorylated by ATM at serine 824 in response to DNA damage. The interplay between HP1-KAP1 binding/ATM phosphorylation during DNA repair is not known. We show that HP1α and unmodified KAP1 are enriched in endogenous heterochromatic loci and at a silent transgene prior to damage. Following damage, γH2AX and pKAP1-s824 rapidly increase and persist at these loci. Cells which lack HP1 fail to form discreet pKAP1-s824 foci after damage but levels are higher and more persistent. KAP1 is phosphorylated at Serine 473 in response to DNA damage and its levels are also modulated by HP1. Unlike pKAP1-s824, pKAP1-s473 does not accumulate at damage foci but is diffusely localized in the nucleus. While HP1 association ...
Objective: The objective of this selective EBM review is to determine whether or not dasatinib improves outcomes and tolerability in patients with chronic myeloid leukemia as compared to imatinib. Study Design: Review of three English language, non-blinded randomized controlled trials from 2009, 2010, and 2010. Data Sources: Randomized, controlled, non-blinded clinical trials comparing dasatinib to imatinib or comparing dasatinib once daily vs dasatinib twice daily, found using the PubMed database. Outcomes measured: Overall survival and progression-free survival were measured at one and two years after initiation of therapy. Safety profiles and incidence of adverse effects were also measured. This is graded on a scale of 1 to 4, from lowest in severity to highest in severity. Additionally, adverse effects were noted as hematologic (neutropenia, anemia, thrombocytopenia) or nonhematologic (fluid retention, diarrhea, vomiting, fever). Results: When comparing dasatinib to imatinib, both drugs provided
A new Korn Ferry (NYSE:KFY) survey of Chief Human Resource Officers (CHROs) shows that as the HR function becomes more strategic and high-profile, HR
Publications List Abstract: These posters are available for download in Adobe Acrobat PDF file format:Discrimination Is Illegal Poster in English (PDF)Discrimination Is Illegal Poster in Spanish (PDF)Sexual Harassment Poster in English (PDF)Sexual Harassment Poster in Spanish (PDF)
Chronic Kidney Disease (CKD) is divided into 5 stages from stage 1 to stage 5. If you have kidney disease, your kidneys are slowly losing their ability to remove wastes and excess water from your blood3 kidney disease indicates moderate chro
In the snowy March of 2003, I climbed Slide Mountain, the tallest of the Catskill range at 4,180 feet, and met a wild-looking man named Sean McFall, who was staying 35 days on Slide s shoulders, in the three-foot snow drifts, with the ice blowing from the treetops and his demonic-looking white bulldog keeping him warm when the temperature dropped to minus 20 degrees Fahrenheit.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
VivoTag 680 XL Fluorochrome ideal for labeling nanoparticles and macromolecules. Hydrolytically stable and low self quenching for higher loading applications.
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Figure 3 - Phenogram of overall molecular similarity of RAPD bands among specimens from all localities at Tibagi, Paraná, and Iguaçu Rivers. Codes as in Figure 1. DISCUSSION The RAPD-PCR patterns indicate strong genetic differentiation within and among populations in the Paraná and the Tibagi Rivers. In the Paraná River at Porto Rico, the high levels of allelic polymorphism may represent the pristine composition of different species or populations within the H. malabaricus species complex, or they may be the outcome of faunal mixing caused by the closing of the Itaipu Dam in 1978 and the consequent flooding of Sete Quedas, another Oligocenic geographic barrier (Sampaio, 1988). Sete Quedas was the natural boundary for the Upper and Middle Paraná basins. The presence of an outlier in the Paraná River similar to the Iguaçu population suggests that the Paraná may indeed be a recipient of trahiras of diverse origins. The Londrina sample from the Tibagi River is most similar to the populations ...
A method is described in which smears on slides, which had been examined previously in a direct fluorescence antibody (DFA) test for Chlamydia trachomatis, were tested by the polymerase chain reaction (PCR). Twenty four (73%) of 33 smears which contained fewer than 10 elementary bodies when examined by the DFA test were positive by the PCR. Of the nine negative smears, seven contained only one or two elementary bodies. However, single elementary bodies were detected by the PCR in seven of the 24 positive samples. Fifteen smears were negative by both methods. The ability to detect small numbers of elementary bodies by the PCR and its specificity for negative smears indicates its potential for retrospective analysis of stored, archival smears on slides.. ...
ID B1VQ72_STRGG Unreviewed; 299 AA. AC B1VQ72; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 10-APR-2019, entry version 33. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG17173.1}; GN OrderedLocusNames=SGR_344 {ECO:0000313,EMBL:BAG17173.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT "Genome sequence of the streptomycin-producing microorganism RT Streptomyces griseus IFO 13350."; RL J. Bacteriol. ...
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Define panchromatism. panchromatism synonyms, panchromatism pronunciation, panchromatism translation, English dictionary definition of panchromatism. adj. Sensitive to all colors: panchromatic film. pan·chro′ma·tism n. the quality or condition of being lsensitive to all colors, as certain types of...
Centocor, Inc. announced today that the U.S. Food and Drug Administration (FDA) has approved Remicade (infliximab) for the treatment of adult patients with chro
Apparatus and methods for automatically staining or treating multiple tissue samples mounted on slides are provided, in which the slides and reagent bottles are held in fixed position, and the reagent and wash solutions brought to the slides.
Creeping Flows. Steven A. Jones BIEN 501 Wednesday, March 21, 2007 Start on Slide 53. Creeping Flows. Major Learning Objectives: Compare viscous flows to nonviscous flows. Derive the complete solution for creeping flow around a sphere (Stokes flow). Slideshow 4702026 by isaiah
Very good quality Brushes set. 7 pcs/pack. Tip No. 0, 2, 4, 6, 8, 10, 12. for dusting microtome cryostat and laying paraffin section in water bath, transfer free floating section into buffer, mounting paraffin, frozen free floating section on slide.
Rapid latex test for qualitative & semiquantitative determination of C-reactive protein in serum by agglutination of latex particles on slide read ...
Note on slide reads "Birch Bay" and "Ed took this." Blurred image of people in a body of water. In the foreground are two males, one wearing a hat is bent over at the waist while the other holds on to him and leans on his back. Near the two males is a woman looking toward the camera and holding up the hem of her dress and something white in her hand. Near the woman is a child. In the distance are what could be swimmers and a shoreline ...
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References for Abcams Recombinant human UCH37 protein (ab108375). Please let us know if you have used this product in your publication
Back in 2004 when Dr. Jane Lemaire became vice-chair of physician wellness in the University of Calgarys department of medicine, the issue was in its infancy.
newera at plaza.snu.ac.kr wrote: , Do not 6M ganidine HCl, 8M urea or some zwitter-ionic detergent make , any difference to cation exchange chromatography or affinity chromatography? As others pointed out already, 6M guanidine will certainly affect your ion exchange chromatography step because of its high ionic strength. However, urea wont. Affinity chromatography on blue sepharose is likely to be affected by both guanidine and urea since it will denature the protein -- affinity chromatography usually needs the native molecule, however. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP3 */ /* Science is the game we play with God to find out what His rules are ...
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There are two automated imaging systems in the Facility that can be used for HCS applications. The first is an InCell 6000 which is a semi-confocal imaging system designed to image cells cultured in standard format multi-well plates (although it can also image cells on slides). In addition to imaging fixed cells, the system has many features which make it suitable for live cell analysis including temperature control, CO2 regulation and liquid handling. The InCell 6000 is equipped with a Caliper Twister II robotic plate loader which significantly increases the throughput capability of the system. Potential applications for this system include live/dead analysis, cell cycle analysis, apoptosis, cytoplasmic/ nucleus translocation assays, DNA damage, tube formation (angiogenesis), neurite outgrowth, micronucleus assays, morphometric analysis (tubulin) and cell migration ...
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Empire Genomics GP9 FISH probe is used to detect translocations of the GP9 gene and can be labeled in one of five colors, using standard nick translation protocols. Each probe is sold in 20 test kits (~20 slides - 22x22 mm area) and includes hybridization buffer. Order 5 or more of the GP9 FISH probe and save 10%!
Empire Genomics TRIM51FP FISH probe is used to detect translocations of the TRIM51FP gene and can be labeled in one of five colors, using standard nick translation protocols. Each probe is sold in 20 test kits (~20 slides - 22x22 mm area) and includes hybridization buffer. Order 5 or more of the TRIM51FP FISH probe and save 10%!
Final nominees for The 53rd Annual CMA Awards will be revealed Wednesday, Aug. 28, with select categories announced live on ABCs
CMA members access CPG Infobase through Joule on cma.ca. In late 2018, this tool-with some additional features-will make the move to a new home on joulecma.ca. Although we anticipate a seamless experience, if you experience any interruption in service, please contact [email protected]/Tel: 888-855-2555 (toll-free).. ...
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Normal cell division requires faithful DNA replication and proper DNA segregation in order to generate two identical daughter cells. Within cells DNA is always assembled with positively charged histones to form protein-DNA packaging structures called chromatin. The order of chromatin packaging regulates cellular functions including replication, gene expression and chromosome segregation. The compact chromatin region is called heterochromatin. The centromere is a rigid, gene-silent heterochromatin region and is essential for faithful chromosome segregation. To duplicate a cell, this region needs to be unpacked for replication and further be reassembled to maintain its function. I tackled how cells maintain faithful replication and reassembly at this region in S. pombe. ❧ I found that a replication protein Cdc18/Cdc6 associates with heterochromatin protein Swi6/HP1. A mutation cdc18-I43A that reduces Cdc18 association with Swi6 has no silencing defect at the centromere, but changes Swi6 ...
Fig. 16.20 Chro matic aberration splits white light into its component spectral colors. Red is refracted least, and blue is refracted most.. Fig. 16.20 Chro matic aberration splits white light into its component spectral colors. Red is refracted least, and blue is refracted most.. Patients may report being able to see better when looking through a disk with a pinhole (a stenopeic aperture) than without it. This usually is a sign of an uncompensated refractive error in the eye.. The further peripherally the light ray strikes the lens, the more it will be refracted (Fig. 16.21). The iris intercepts a large share of these peripheral light rays. A narrow pupil will intercept a particularly large share of peripheral light rays, which improves the depth of field. Conversely, depth of field is significantly poorer when the pupil is dilated.. H Patients who have received mydriatic agents should refrain from driving.. H Patients who have received mydriatic agents should refrain from driving. ...
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Like tortoiseshell and calico cats, patched tabbies are nearly always female because the genes that trigger the tortie pattern require two X chromosomes (the red fur gene on one and the black fur gene on the other). Because males have only one X chromosome (the typical male pattern is XY), they cant produce both red and black patches in the same coat except in the case of a genetic abnormality that gives the male an extra X chro. mosome. However, such males are rare (Rubin, 2009).. Ghost Tabby Markings on Kittens. Kittens of various breeds may be born with ghost tabby markings, or very faint stripes and/or marbled patches. These typically disappear or at least fade significantly as the cat ages (see the photos below for a picture of a kitten with ghost tabby markings).. For more cat articles, see the main Cats page.. ...
Uch37 is a de-ubiquitylating enzyme that is functionally linked with the 26S proteasome via Rpn13, and is essential for metazoan development. Here, we report the X-ray crystal structure of full-length human Uch37 at 2.95 Å resolution. Uch37's catalytic domain is similar to those of all UCH enzymes characterized to date. The C-terminal extension is elongated, predominantly helical and contains coiled coil interactions. Additionally, we provide an initial characterization of Uch37's oligomeric state and identify a systematic error in previous analyses of Uch37 activity. Taken together, these data provide a strong foundation for further analysis of Uch37's several functions ...
A method is provided for amplifying and detecting specific GC-rich nucleic acid sequences contained in a nucleic acid or in a mixture of nucleic acids, which includes treating a separate nucleic acid containing the specific sequence with a molar excess of primers and a polymerase and extending the primers in the presence of dATP, dCTP, TTP, and an analogue of dGTP. In one application of the present invention, individuals who are carriers for, or afflicted by, the fragile X syndrome are detected.
DNA sequencing is an invaluable tool for studying medical and biological systems. The genetic components of many diseases have been discovered, which holds great promise for new treatment and drug discovery for treat diseases such as cancer. However, the sequencing instruments of today are mainly research instruments and few have been developed for clinical diagnosis at a decentralized clinic. Therefore there is a need of a complementary small-scale sequencing platform that does not require trained personnel and expensive lab equipment. This project aims to contribute to that development by investigating and finding the optimal enzymatic reaction conditions for small-scale single nucleotide sequencing. The first part was to investigate the optimal conditions for on-slie extension and detection. Probes were printed on a CodeLink array followed by hybridisation to a primer-target complex. Extension was done on slide by the polymerase Klenow exo- with specific biotinylated nucleotides. The signals ...
But sometimes they can be either bright pink, or pale yellow, as in these next two. The pink one I took many years ago, on slide film. A pink one was seen by several people late this summer, in the vegetation right next to the Rondeau Visitor Centre. The yellow one I photographed on Pelee Island a few years ago on a digital P&S. Both have been used in the book Songs Of Insects, an excellent book illustrating the numerous crickets, katydids, cicadas, etc of eastern North America. It includes a CD with the various insect songs and is available at the Friends of Rondeau bookstore ...
A 20-year-old man was killed almost instantly when his car left the road and smashed through a wooden fence before coming to rest in a field, an inquest has found.
This for Ely .This is a case of a written parking space agreement that was taken away from a 40 year Tenant who has - Answered by a verified Real Estate Lawyer
... fluorochrome is a high quality APC-cyanine tandem for the red diode-laser designed to achieve the best performance for all the available conjugated antibodies. This fluorochrome is compatible with any flow cytometer equipped with a red diode-laser and the appropriate filters and detectors for the infrared signal. Cytognos offers a wide portfolio of APC-C750 conjugates as well as a custom-conjugation service ...
SuWang Word to Chm is a handy tool that can quickly convert a word document to chm according to the outline structure generated chm file and html sites for WindowsXP 2003 Vista Vista64 7
Numerous reports have suggested that disturbances in nuclear condensation may result in male infertility. This notion has been supported by the observation of infertile individuals with a decrease or absence of the male sperm-specific chromatin packaging protamine proteins. To date, no correlation between the absence of protamine proteins and a mutation within the coding regions of the protamine genes has been documented. To address this issue, PCR-based mutation scanning analysis has been performed across the human male haploid expressed PRM1--|PRM2--|TNP2 domain in several oligozoospermic infertile individuals. This analysis identified a candidate mutation in a region of contact with the sperm nuclear matrix from 2 of 5 affected individuals. This is the first report of a mutation scan covering the entire PRM1--|PRM2--|TNP2 locus in affected individuals.
Acute and chronic pain present major medical problems worldwide, having negative impacts on human health. Current analgesic therapies provide incomplete pain relief and side effects in many patients, limiting their clinical utility. Peptide-based drug candidates offer several advantages over small molecule pharmaceuticals. The primary obstacles hindering development of peptide-based drugs have been related to pharmacokinetic issues including oral bioavailability, serum stability and poor penetration of the blood-brain barrier (BBB). A primary goal of this dissertation was to further advance strategies to improve delivery of peptide-based drugs into the CNS. Previous work demonstrated that glycosylation of small enkephalin-based peptides increases stability and BBB penetration. Glycosylation and phosphorylation of opioid peptides were further investigated. Both strategies increased the water solubility of the parent peptide but did not decrease receptor affinity for mu or delta opioid receptors. The
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The RNAscope® 2.5 HD Duplex Assay is based on ACDs patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in FFPE samples mounted on slides.
The combination of analytic software advances with microscope features provides the iCys with the ability to automatically move the microscope stage to another region of interest after scanning and analysis are complete. The event can then be viewed using the microscope optics, while simultaneously viewing the laser scan images. The user may further utilize any number of microscope accessories available, including optomechanical devices for micromanipulation and cell capture. The iCys displays the 3D morphology of the specimen with protocol-driven settings for variable angle laser scatter along with absorbance direction. The variable resolution scanning yields the ability to scan larger areas for higher throughput analysis. This produces the best resolution of the analysis without compromising sensitivity. The iCys also combines scanned imaging with live visualization.. Since the specimens under study are on slides, they can be measured repeatedly over time, which is ideal for the study of ...
Figs 6 11. Terpides (Fittkaulus) amazonicus (holotype): 6 tergalius I; 7 tergalius II; 8 exiviae of abdominal tergum VI with tergalius (cuticular pigmentation shown by dots); 9 tergalius VII; 10 head of male imago; 11 abdomen of male imago spread on slide (hypodermal pigmentation shown by dots).. ...
This compilation of fluorochrome data compiles peak excitation and emission wavelengths and is arranged alphabetically by fluorochrome.
Our Radiolabeling Department can transcribe any plasmid templates or PCR amplified DNA fragments by either Nick Translation, Random Priming, Klenow fill-in, T4-PNK or Terminal Transferase and label the RNA trasnscripts with 32P, 33P, 35S, biotin, or digoxigenin (DIG).. Methods have been developed and validated in our laboratory for the production of probes for use in In Situ Hybridization experiments.. ...
Info concerning Ely MN critical care nursing. You can enter nursing with either an associates degree or BSN. As a licensed vocational nurse (LVN), you may provide patient care under the supervision of a registered nurse (RN).
The ultimate guide to Ely, Minnesota - Gateway to the Boundary Waters Canoe Area Wilderness (BWCAW) © 2018. ElyMinnesota.com. All rights reserved. Designed by Lutefisk Technologies ...
Visit and receive a free informational tour of the historic Ely Cemetery, private burial ground of generations of the Ely family and other prominent families. Established in 1777 by Captain William Ely, the last person interred there was an eighth-generation descendent of Captain Ely, buried there in 1978 ...
Detail záznamu - An Improved Consensus Linkage Map of Barley Based on Flow-Sorted Chromosomes and Single Nucleotide Polymorphism Markers - Detail záznamu - Knihovna Akademie věd České republiky
Nine KOX zinc finger genes were localized on four human chromosomes by in situ hybridization of cDNA probes to metaphase chromosomes. KOX1 (ZNF10), KOX11 (ZNF18), and KOX12 (ZNF19) were mapped to chro
Hairy cell leukemia (HCL) is a chronic lymphoid leukemia, originally described in 1958 by Bouroncle and colleagues and named after the hairlike cytoplasmic projections seen on the surface of the abnormal B-cells (see the image below).{file36451}See Chronic Leukemias: 4 Cancers to Differentiate, a Critical Images slideshow, to help detect chro...
I am a 25 year old woman who suffers from various health conditions including severe hypermobility syndrome, fibromyalgia, raynauds disease, somatoform pain disorder, flat feet/plantation faticis, chro...
Chromomycin A3. *Chaparonin. *Chitin. *α-Chloralose. *Clóraifill b2 Chlorophyll. *Cholecystokinin (CCK). *Colaistéaról ...
... or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of ...
... chromomycin a3 MeSH D09.408.320.500 --- methylgalactosides MeSH D09.408.320.550 --- nitrophenylgalactosides MeSH D09.408. ...
C19H42BrN Chelerythrine Chromomycin A3 Chaparonin Chitin α-Chloralose Chlorophyll Cholecystokinin (CCK) Cholesterol Choline ...
Chromomycin A3 or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of ...
A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic ... NMR STRUCTURE OF D(TTGGCCAA)2 BOUND TO CHROMOMYCIN-A3 AND COBALT. ...
A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic ... AVERAGE SOLUTION STRUCTURE OF D(TTGGCCAA)2 BOUND TO CHROMOMYCIN-A3 AND COBALT. ...
Literature References: Major component of an antitumor antibiotic complex produced by Streptomyces griseus. Binds specifically to guanine-cytosine base pairs in DNA; does not intercalate. The aglycone, chromomycinone, is identical to that of plicamycin, q.v. Isoln of complex and antibacterial activity: M. Shibata et al., J. Antibiot. 13B, 1 (1960), C.A. 54, 22835g (1960). Characterization: K. Mizuno, J. Antibiot. 16A, 22 (1963). Structure: M. Miyamoto et al., Tetrahedron 23, 421 (1967). Abs config: N. Harada et al., J. Am. Chem. Soc. 91, 5896 (1969). Revised structure: J. Thiem, B. Meyer, J. Chem. Soc. Perkin Trans. 2 1979, 1331. Pharmacology and toxicity: M. Slavik, S. K. Carter, Adv. Pharmacol. Chemother. 12, 1 (1975). Fluorescence characteristics: R. H. Jensen, J. Histochem. Cytochem. 25, 573 (1977). Use for analysis and identification of chromosomes: J. H. van de Sande et al., Science 195, 400 (1977); in high-speed chromosome sorting: J. W. Gray et al., ibid. 238, 323 (1987); in flow ...
... ,3D-O-(4-O-acetyl-2,6-dideoxy-3-C-methyl-alpha-L-arabino-hexopyranosyl)-7-methylolivomycin D,aburamycin B, ...
title = "Quantitative footprinting analysis of the chromomycin A3-DNA interaction",. abstract = "Chromomycin A3 (CHR) binding ... N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using ... AB - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using ... Stankus, A, Goodisman, J & Dabrowiak, JC 1992, Quantitative footprinting analysis of the chromomycin A3-DNA interaction, ...
chromomycin A3. DAPI. 4′,6-diamidino-2-phenylindole. FISH. fluorescence in-situ hybridization ...
chromomycin A3 A glycosidic antineoplastic antibiotic isolated from the bacterium Streptomyces griseus. Chromomycin A3 ... cereblon E3 ubiquitin ligase modulating agent CC-92480 A modulator of the E3 ubiquitin ligase complex containing cereblon (CRL4 ... A modulator of cereblon (CRBN), which is part of the cullin 4-RING E3 ubiquitin ligase complex (CRL4-CRBN E3 ubiquitin ligase; ... Upon administration, cereblon E3 ubiquitin ligase modulating agent CC-92480 specifically binds to cereblon (CRBN), thereby ...
To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... and A3 (2). 1 and 2 showed potent cytotoxicity against the human gastric adenocarcinoma (AGS) cell line (IC50 1; 1.7 and 2; ... Keywords: chromomycin A2; chromomycin A3; actinomycetes; TRAIL; Wnt chromomycin A2; chromomycin A3; actinomycetes; TRAIL; Wnt ...
Chromomycin A3. 450. 570. B, BV. G-C Range. 4, 6-Diamidino-2-. Phenylindole HCl (DAPI). 372. 456. U. A-T Range. ...
Crystal structure of the [Co2+-(chromomycin A3)2]-d(CCG)3 complex. ... CoII(Chromomycin)2 Complex Induces a Conformational Change of CCG Repeats from i-Motif to Base-Extruded DNA Duplex. Int J Mol ...
Basic and Clinical Aspects of Sperm Chromomycin A3 Assay Gian Carlo Manicardi, Davide Bizzaro, Denny Sakkas ...
Role of magnesium ion in the interaction between chromomycin A3 and DNA: Binding of chromomycin A3-Mg2+ complexes with DNA. ... Chromomycin A3(Chro). The anticancer antibiotic chromomycin A3 (Chro), a DNA minor groove binding drug which interferes with ... Slavik, M.; Carter, S.K. Chromomycin A3, mithramycin, and olivomycin: Antitumor antibiotics of related structure. Adv. ... chromomycin A3 with DNA: Does neutral antibiotic bind DNA in absence of the metal ion? J. Biomol. Struct. Dyn 2000, 18, 209-218 ...
Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol ...
NMR studies of chromomycin A3 interaction with DNA. Biochemistry. 1985 Nov 19; 24(24):6887-93. ...
... chromomycin A3 and silver staining ... Chromomycin A3 stains nucleolar organizer regions of fish ...
Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol ... discrepancy between chromomycin and aniline blue staining. Reprod Biomed Online. (2009) 19:264-9. doi: 10.1016/S1472-6483(10) ...
Of those, chromomycin-a3, fulvestrant and apicidin were active in nanomolar doses in all the three strains tested, with the ... Chromomycin-a3 (AC50 = 0.011 µM), fulvestrant (0.0667 µM), apicidin (0.078 µM), ingenol (1.4408 µM) and tacrolimus (2.4383 µM) ... In this study, the highest active single agents were apicidin, fulvestrant and chromomycin-a3. The only single agent that is ... The results of the screen are summarised in Table 1, where chromomycin-a3 (CHR), fulvestrant (FUL), apicidin (API), ingenol ( ...
本研究題目主要是以抗癌藥與目標物的結合機制及其在癌症疾病的治療作一系列的探討。抗生素Mithramycin 和Chromomycin A3 是一種鍵結於DNA的aureolic 類抗癌藥物。 其活性主要來自於干擾DNA的複製及轉錄作用,並運用於臨床的癌症 ... Chen, Y.W. and Hou,M.H.(2013) The binding of the Co(II) complex of dimeric chromomycin A3 to GC sites with flanking G:G ... Chen, Y.W. and Hou,M.H.(2013). The binding of the Co(II) complex of dimeric chromomycin A3
Silva DJ, Goodnow R, Kahne D. The sugars in chromomycin A3 stabilize the Mg(2+)-dimer complex. Biochemistry. 1993 Jan 19; 32(2 ... design and synthesis of a ligand based on chromomycin A3. Bioorg Med Chem. 1994 Nov; 2(11):1251-9. PMID: 7757421. ...
Fluorescence R-banding with chromomycin A3 and methyl green was performed as described in detail earlier (53). Whenever ...
Chromomycin A3 (CMA3) is a fluorescent dye that intercalates into DNA but because of its size it can only bind to sperm DNA ... 2009). Use of chromomycin A3 staining in bovine sperm cells for detection of protamine deficiency. Biotech. Histochem. 84, 79- ... chromomycin A3 (CMA3) staining, and for isolation of protein for AU-PAGE, or were frozen. ...
Normal sperm morphology and chromatin packaging: Comparison between aniline blue and chromomycin A3 staining Franken D.R.; ...
... chromomycin A3 and Aniline blue staining).. RESULTS: After 35 days, in addition to above mentioned sperm parameters, all of the ...
Two helium-cadmium (HeCd) lasers, emitting 16 mW at 442 nm and 35 mW at 325 nm, were used to excite chromomycin A3 ... ... The analysis of chromosomes was performed through Ag-staining, C-banding, chromomycin A3 and DAPI staining, and fluorescent in ... In the present investigation chromosomal preparations of Asellus aquaticus were sequentially stained with chromomycin A3 to ... Quinacrine and distamycin-DAPI, which selectively stain AT-rich DNA, and chromomycin, specific for GC-rich sequences, were used ...