Glycosidic antibiotic from Streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.
A complex of several closely related glycosidic antibiotics from Streptomyces griseus. The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. It is also used as an antineoplastic agent, especially for solid tumors.
A mixture of several closely related glycosidic antibiotics obtained from Actinomyces (or Streptomyces) olivoreticuli. They are used as fluorescent dyes that bind to DNA and prevent both RNA and protein synthesis and are also used as antineoplastic agents.
A tricyclic pentaglycosidic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA. It is used as a fluorescent dye and as an antineoplastic agent, especially in bone and testicular tumors. Plicamycin is also used to reduce hypercalcemia, especially that due to malignancies.
An actinomycete from which the antibiotics STREPTOMYCIN, grisein, and CANDICIDIN are obtained.
A mitosporic fungal genus. Phialophora verrucosa is a cause of chromomycosis (CHROMOBLASTOMYCOSIS). Ophiobolus is the teleomorph of Phialophora.
Oligopeptide antibiotics from Streptomyces distallicus. Their binding to DNA inhibits synthesis of nucleic acids.
A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)
Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Elements of the lanthanoid series including atomic number 57 (LANTHANUM) through atomic number 71 (LUTETIUM).
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
Plant-eating orthopterans having hindlegs adapted for jumping. There are two main families: Acrididae and Romaleidae. Some of the more common genera are: Melanoplus, the most common grasshopper; Conocephalus, the eastern meadow grasshopper; and Pterophylla, the true katydid.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Any method used for determining the location of and relative distances between genes on a chromosome.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
A class of ciliate protozoa. Characteristics include the presence of a well developed oral apparatus and oral cilia being clearly distinct from somatic cilia.
Environments or habitats at the interface between truly terrestrial ecosystems and truly aquatic systems making them different from each yet highly dependent on both. Adaptations to low soil oxygen characterize many wetland species.
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
Places for cultivation and harvesting of fish, particularly in sea waters. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Examination of CHROMOSOMES to diagnose, classify, screen for, or manage genetic diseases and abnormalities. Following preparation of the sample, KARYOTYPING is performed and/or the specific chromosomes are analyzed.
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
Mapping of the KARYOTYPE of a cell.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
A publication issued at stated, more or less regular, intervals.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Experimentation on STEM CELLS and on the use of stem cells.
The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
A nonsteroidal anti-inflammatory agent with analgesic properties used in the therapy of rheumatism and arthritis.
A count of SPERM in the ejaculum, expressed as number per milliliter.
Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.
The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.

Chromomycin A3 staining as a useful tool for evaluation of male fertility. (1/49)

PURPOSE: Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate. METHODS: Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA3 positivity is expressed as the percentage in 200 spermatozoa. RESULTS: Percentage of CMA3 positivity showed not only a negative correlation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover, percentage of CMA3-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA3 positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA3-positive semen samples in comparison with low CMA3-positive semen samples. CONCLUSION: There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.  (+info)

The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. (2/49)

Human semen is heterogeneous in quality, not only between males but also within a single ejaculate. Differences in quality are evident, both when examining the classical parameters of sperm number, motility and morphology and in the integrity of the sperm nucleus. The aim of this study was to determine the efficiency of the PureSperm((R)), Percoll((R)) and swim-up preparation techniques to eliminate spermatozoa with nuclear anomalies. Semen samples were collected, washed and one part of the semen spread on a slide, the remainder was prepared using the swim-up, PureSperm((R)) or Percoll((R)) techniques. Spermatozoa from different fractions were fixed on slides and assessed. Sperm samples (n) from different men were stained using the chromomycin A(3) (CMA(3)) fluorochrome, which indirectly demonstrates a decreased presence of protamine (n = 31 for swim-up; n = 45 for PureSperm((R)); n = 39 for Percoll((R))). Spermatozoa prepared using PureSperm((R)) (n = 35) and Percoll((R)) (n = 37) were also examined for the presence of endogenous DNA nicks. Good quality spermatozoa should not possess DNA nicks and not stain (i.e. fluoresce) with CMA(3). When prepared using the swim-up technique the spermatozoa recovered showed no significant improvement with the CMA(3) staining. When spermatozoa were prepared using the PureSperm((R)) and Percoll((R)) techniques, a significant (P < 0.001) decrease in both CMA(3) positivity and DNA strand breakage was observed. These results indicate that both the PureSperm((R)) and Percoll((R)) techniques can enrich the sperm population by separating out those with nicked DNA and with poorly condensed chromatin.  (+info)

Evaluation of complexation of metal-mediated DNA-binding drugs to oligonucleotides via electrospray ionization mass spectrometry. (3/49)

The interactions of self-complementary oligonucleotides with a group of metal-mediated DNA-binding drugs, including chromomycin A(3), mithramycin and the novel compound UK-1, were examined via electrospray ionization quadrupole ion trap mass spectrometry. Both chromomycin and mithramycin were shown to bind preferentially to GC-rich oligonucleotide duplexes in a 2:1 drug:metal ratio, while UK-1 was shown to bind in a 1:1 drug:metal stoichiometric ratio without a strong sequence preference. These trends were observed in the presence of Co(2+), Ni(2+) and Zn(2+), with the exception that chromomycin-Zn(2+) complexes were not readily observed. The binding stoichiometries as well as the sequence specificities are in agreement with literature reports for solution studies. Binding selectivities and stabilities of the complexes were also probed using electrospray ionization mass spectrometry. Both of the GC-rich oligomers 5'-GCGCGC-3' and 5'-GCGCATGCGC-3' exhibited a binding preference for chromomycin over mithramycin in the presence of Co(2+) and Ni(2+). Energy-variable collisionally activated dissociation of the complexes was employed to determine the stabilities of the complexes. The relative metal-dependent binding energies were Ni(2+) > Zn(2+) > Co(2+) for UK-1-oligomer complexes and Ni(2+) > Co(2+) for both mithramycin and chromomycin complexes.  (+info)

Homolog pairing and two kinds of bouquets in the meiotic prophase of rye, Secale cereale. (4/49)

Chromosome configurations and structures during meiotic prophase were investigated by staining large repeated DNA sequences localized in the subtelomeric regions of all the chromosomes in rye, Secale cereale, in order to clarify when and how homolog pairing and bouquet formation occur. The changes of the spatial locations of chromosomes in the nucleus were investigated by the use of laser confocal microscopy, together with the surface-spreading method of silver nitrate staining to detect the formation of the synaptonemal complex. Homolog pairing in which homologs of four chromatids of a pair of homologs were coaligned in parallel but remained distinctly separate was microscopically detected for the first time in the present study. Homolog pairing showed the following characteristics: (1) it occurred at the leptotene-zygotene transition stage, prior to the formation of nodules and the synaptonemal complex; (2) the chromatin structure of chromosomes was in a state of decondensation; (3) it required no telomere clustering. These data suggest that homolog pairing represents a structure that indicates incipient recombination. After the homolog pairing stage, two kinds of bouquet configuration were found in zygotene. The commonly observed type was a loose bouquet, in which the subtelomeric regions were loosely aggregated. The other type was a definite bouquet, in which almost all the subtelomeric regions were conjugated, but this type was observed only in a limited number of the meiotic prophase cells of some individuals. It was concluded that the former represents the configuration of homologous recombination and the latter that of ectopic recombination.  (+info)

Crystal structure of the [Mg2+-(chromomycin A3)2]-d(TTGGCCAA)2 complex reveals GGCC binding specificity of the drug dimer chelated by a metal ion. (5/49)

The anticancer antibiotic chromomycin A3 (Chro) is a DNA minor groove binding drug belonging to the aureolic family. Chro likely exerts its activity by interfering with replication and transcription. Chro forms a dimer, mediated by a divalent metal ion, which binds to G/C-rich DNA. Herein we report the first crystal structure of Chro bound to d(TTG GCCAA)2 DNA duplex solved by multiwavelength anomalous diffraction (MAD) based on the chelated Co3+ ion. The structure of the Mg2+ complex was subsequently refined at 2.15 A resolution, which revealed two complexes of metal-coordinated dimers of Chro bound to the octamer DNA duplex in the asymmetric unit. The metal ion is octahedrally coordinated to the O1 and O9 oxygen atoms of the chromophore (CPH), and two water molecules act as the fifth and sixth ligands. The two coordinated water molecules are hydrogen bonded to O2 atoms of C5 and C13 bases. The Chro dimer binds at and significantly widens the minor groove of the GGCC sequence. The long axis of each chromophore lies along and stacks over the sugar-phosphate backbone with the two attached saccharide moieties (rings A/B and C/D/E) wrapping across the minor groove. DNA is kinked by 30 degrees and 36 degrees in the two complexes, respectively. Six G-specific hydrogen bonds between Chro and DNA provide the GGCC sequence specificity. Interestingly, DNA in concert with Chro appears to act as an effective template to catalyze the deamination of Co(NH3)6(3+), as shown by circular dichroism and crystal structure data. Our results present useful structural information for designing new anticancer drug derivatives in the future.  (+info)

Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (6/49)

The biosynthetic gene cluster of the aureolic acid type antitumor drug chromomycin A3 from S. griseus subsp. griseus has been identified and characterized. It spans 43 kb and contains 36 genes involved in polyketide biosynthesis and modification, deoxysugar biosynthesis and sugar transfer, pathway regulation and resistance. The organization of the cluster clearly differs from that of the closely related mithramycin. Involvement of the cluster in chromomycin A3 biosynthesis was demonstrated by disrupting the cmmWI gene encoding a polyketide reductase involved in side chain reduction. Three novel chromomycin derivatives were obtained, named chromomycin SK, chromomycin SA, and chromomycin SDK, which show antitumor activity and differ with respect to their 3-side chains. A pathway for the biosynthesis of chromomycin A3 and its deoxysugars is proposed.  (+info)

Human cervical mucus can act in vitro as a selective barrier against spermatozoa carrying fragmented DNA and chromatin structural abnormalities. (7/49)

PURPOSE: We have carried out experiments to determine if human cervical mucus can act as an in vitro selective barrier against spermatozoa morphologically normal that carry genetic structural abnormalities. METHODS: Sperm chromatin abnormalities have been evaluated by Chromomycin A3 and "endogenous" nick translation. RESULTS: The data obtained have shown that spermatozoa possessing higher levels of DNA protamination are more proficient in crossing the cervical mucus barrier. Moreover, the levels of positivity to endogenous nick translation treatment was practically zero in such spermatozoa. CONCLUSIONS: We suggest that sperm penetration of cervical mucus could be used to select sperm preparations free of fragmented DNA or chromatin structural abnormalities for assisted reproduction.  (+info)

Cytogenetic studies in three Pimelodella meeki populations (Pisces, Pimelodidae) from Tibagi River basin (Brazil). (8/49)

We analyzed cytogenetically specimens of Pimelodella meeki from Tibagi River at Limoeiro (LM) and from two tributaries, Couro do Boi (CB) and Gabriel da Cunha (GC) Rivers. All specimens presented 2n=46 chromosomes, which were the karyotypes composed by 15 pairs metacentric (M) + 6 pairs submetacentric (SM) + 2 pairs subtelocentric (ST). In specimens of GC, CB, and LM, the results of analyses of the nucleolus organizer regions (NORs), done by means of AgNO3 and CMA3 staining, showed that they are identical, located in terminal position on the short arm of a SM chromosome pair, and they were observed to be a size heteromorphism in some metaphase plates. FISH with 18S rDNA probe yielded evidence for these regions but not for the size variation, indicating that they are not due to a greater number of NOR cistrons in one of the homologue chromosomes. An interesting characteristic of these regions is that they could appear divided in blocks, as evidenced by all the techniques. This work makes clear the necessity for more deeply systematic studies, because of the confused taxonomic situation of the genus Pimelodella.  (+info)

TY - JOUR. T1 - Quantitative footprinting analysis of the chromomycin A3-DNA interaction. AU - Stankus, Allison. AU - Goodisman, Jerry. AU - Dabrowiak, James C.. PY - 1992. Y1 - 1992. N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5′-TGGCCA-3′, 3′-ACCGGT-5′ in the 18-mer with a binding constant of (2.7 ± 1.4) × 107 M-1. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is ∼ 105 M-1. Since it has been suggested that CHR binding alters DNA to the A configuration, quantitative footprinting studies using dimethyl sulfate, which alkylates at N-7 of guanine in the major groove, ...
15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004 ...
Meenakshi Priyam has joined udaan.com- a B2B trade platform, created specifically for small & medium businesses in India - as group CHRO. She has moved from GlaxoSmithKline (GSK), the pharmaceutical major, where she was CHRO for India and global HR head for classic and established products (CEP).. Priyam had spent just over three years at GSK, where she initially headed HR for India and South Asia for almost two years before being elevated to CHRO India & global HR head CEP in May last year.. Before joining GSK, Priyam had served as head HR - global product strategy & commercialisation (GPS&C) and global functions at Novartis, based out of Basel Area, Switzerland. She had joined Novartis in 2013, as head-HR, in Mumbai, before moving to Switzerland in 2016, for a year and four months.. Her longest stint was with Johnson & Johnson (J&J) where she joined as senior HR business partner in 2006. In less than three years, she worked her way up to become the Total Rewards Lead for two years and three ...
Various approaches are used to study the chromosomal makeup of cells. Traditional cytogenetic methods are based on the analysis of mitotic cells fixed onto slides to analyze their chromosomal composition (karyotype) by microscopy. This approach can be combined with FISH to detect specific sequences on morphologically distinct individual chromosomes. Disadvantages of this type of microscopic analysis are the amount of time and labor required to acquire and analyze typically less than a hundred cells. As a result, the statistical power of this type of analysis is limited. An alternative to traditional cytogenetic methods is flow karyotyping (1,2) a method to analyze chromosomes in suspension by flow cytometry. For bivariate flow karyotyping, the DNA composition of specific chromosomes in suspension is measured based on the DNA-specific dyes Hoechst 33258 and chromomycin A3 (3,4). In our protocol, we combine flow karyotyping and FISH to analyze repetitive DNA in individual chromosomes by flow ...
TY - JOUR. T1 - In silico, spectroscopic, and biological insights on annelated pyrrolo[3,2-e]pyrimidines with antiproliferative activity. AU - Terenzi, Alessio. AU - Barone, Giampaolo. AU - Gennaro, Giuseppe. AU - Martorana, Annamaria. AU - Almerico, Anna Maria. AU - Gentile, Carla. AU - Lauria, Antonino. PY - 2014. Y1 - 2014. N2 - The in silico COMPARE analysis was performed on 8-[3-(piperidino)propyl]-4,10-dimethyl-9-phenyl-6-(methylsulfanyl)-3,4-dihydropyrimido[1,2-c]pyrrolo[3,2-e]pyrimidin-2(8H)-one, a compound with promising antiproliferative activity, previously synthetized and screened against a panel of 60 human tumor cell lines. The results evidenced that this compound matches the biological properties of Chromomycin A3 and Actinomycin D, known drugs with high DNA binding affinity. Prompted by such results, a thorough spectroscopic investigation of its DNA aqueous solutions was performed, with the aim to verify its DNA-binding properties. DNA groove-binding interaction was assigned by ...
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1EKH: A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance.
Vikram Duggal joins as the VP and head HR for pharma solutions, while Unmesh Rai is the new head-employer branding and talent acquisition.. Pharma major Piramal Group is all set for an HR overhaul. The company now has a new CHRO in Vikram Bector, who joined a few months ago.. Following this, the company has made two new senior level appointments in HR department. Vikram Duggal has joined as the VP and head-HR for pharma solutions, and Unmesh Rai is the new head employer branding and talent acquisition.. Duggal and Rai will both work with Vikram Bector, who joined the company as the Group CHRO this July. Bector, who is driving the HR Transformation 2.0 at the Piramal Group, has vast experience in building world-class HR practices. He is well known for his expertise in leading HR transformations on a large scale; as well as for being a thought leader and a leadership coach.. Even as the Piramal Group accelerates its HR transformation through the Strategy for Employee Engagement and Development ...
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Streptomyces griseus subsp. griseus bacteriophage 22653 ATCC ® 11984-B1™ Designation: 22653 TypeStrain=False Application:
Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for production of these molecules by manipulating the regulatory genes. A putative but silent chromomycin biosynthetic gene cluster was discovered in Streptomyces reseiscleroticus. Heterologous expression of the ketosynthase, chain length factor, and acyl carrier protein in Streptomyces lividans confirmed that they are responsible for the assembly of a decaketide. Two regulatory genes are present in this gene cluster, including SARP-type activator SrcmRI and PadR-like repressor SrcmRII. Either overexpression of SrcmRI or disruption of SrcmRII turned on the biosynthetic pathway of chromomycins. The production titers of chromomycin A3/A2 in R5 agar in these two strains reached 8.9 ± 1.2/13.2 ± 1.6 and 49.3 ± 4.3/53.3 ± 3.6 mg/L, respectively. An engineered strain was then constructed with both SrcmRII
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Plicamycin (INN, also known as mithramycin; trade name Mithracin) is an antineoplastic antibiotic produced by Streptomyces plicatus. It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently reported in different places all with the same chromomycin core, but with different stereochemistry in the glycoside chain, a 1999 study has re-investigated the compound and proposed a revised structure. Plicamycin has been used in the treatment of testicular cancer, Pagets disease of bone, and, rarely, the management of hypercalcemia. Plicamycin has been tested in chronic myeloid leukemia. Plicamycin is currently used in multiple areas of research, including cancer cell apoptosis and as a metastasis inhibitor. One elucidated pathway shows it interacts by cross-binding chromatin GC-rich promoter motifs, thereby inhibiting gene transcription. Mithramycin A. Fermentek. Wohlert, S. E.; Künzel, E.; Machinek, R.; Méndez, C.; Salas, J. A.; ...
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Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
01/31/05 20:41 PM >>> I need some help!! We have been experiencing some yeast and bacteria showing up on some of our special stains. It started about 2 weeks ago and seems to be sporadic (one day...no problems, the next day, several stains are affected). The yeast and bacteria (rods) are clumped together all over the slide, on the tissue sections, around the sections and on the slide in areas where there is no tissue. The stains we have seen the contamination are the Gram, PAS/D, PAS and GMS. All of these stains are automated except the Gram, so I do not think the special stainer is the problem. Here is what I have eliminated as problem areas: -Processor solutions and paraffin (we cut and stained a block from another institute and had contamination) -Waterbaths, slide drying boards, water containers (used to fill waterbathes), stainer containers, ice pans (all of the mentioned have been thoroughly bleached) -Tap water and DI water (all water supplies have been tested by sending a sample to ...
Ohio States senior class experienced a little bit of everything. The final two years included 24 wins, but two crushing losses.
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Ions as a source of reactants: Ions get neutralized when they reach the surface, they become an additional source of reactive species.. The synergy between ion bombardment and chemical etching was first shown by Coburn and Winters in the classical experiment shown on slide 2.. Besides enhancing the chemical etch, ions also play a major role in removing non-volatile by-products or etch products that require an activation energy to desorp from the surface. The removal of by-products and their redeposition onto the feature sidewall is the fundamental reason why plasma etching can obtain anisotropic profiles (slide 3). Factors that influence the anisotropy are (slide 4 ...
Coho are coming up high on small size spoons and dodger flies. Reapers and Vulcans made by http://www.badgertackle.com have been working for us. The small silver Vulcan has been working on the lead cores. Six inch flashers or dodgers with green or blue Howies peanut flies must be part of your presentation for Coho. Coho are in the top 35 feet. Some action on Slide Divers set to #3 no ring and 35 feet of line out. Tie your flies 14.5 inches on 6 inch dodgers, 24 inches for 6 inch flashers. All orange dodgers and flashers. I hope this helps ...
Disclosed is an automated staining apparatus including an arm moveable in three dimensions, and a hollow tip head located on the arm including integral reagent tip head, wash tip and blow tip for selectively dispensing gas and liquid onto microscope slides. Also disclosed are various sub-components of the apparatus that are specifically adapted to the processing of specimens on slides.
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
Cancer can often be detected in the arrangement of cells in a tissue sample. Once a sample tissue is taken from the patient, it is sent to the histotechnician (HT), who prepares the tiny sections of body tissues for microscopic examination by a pathologist. Working closely with the pathologist, the histologic technician processes tissue biopsies removed during surgery. The tissue is cut into very thin slices, mounted on slides and stained with special dyes to make the cell details visible under the microscope. By examining the section of tissue, the pathologist and the surgeon can learn if disease is present, or if it has spread, and decide the best course of treatment for the patient. The histotechnologist (HTL) has advanced training in how and why specimens are collected and processed for testing. That expertise qualifies the histotechnologist to manage even unexpected situations in the laboratory, such as solving technical or instrument problems, understanding the underlying health and ...
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Even relatively minor errors in chromatin remodeling during spermiogenesis are associated with sperm DNA damage and infertility, yet little is known about the etiology. Mice with severe NPYq deletions are infertile due to severe sperm differentiation defects (Ward and Burgoyne, 2006; Yamauchi et al., 2009). We have recently observed that sperm from these mice presented abnormal chromatin packaging and DNA damage. Moreover, when these sperm were injected into the oocytes, a significant increase of oocyte arrest at pronuclei stage and of chromosome aberrations in the fertilized eggs were noted (Yamauchi et al., 2010). Here we provide evidence that the deficiency of NPYq encoded gene Sly is associated with sperm DNA damage and poor sperm chromatin condensation, and propose that SLY plays a role in spermatid-specific chromatin remodeling.. How can Sly/SLY be involved in sperm DNA damage phenotype? SLY protein has been shown to control the postmeiotic expression of ,100 genes, the majority of which ...
Euphorbia Linnaeus, 1753 (Euphorbiaceae) is one of the most diverse and complex genera among the angiosperms, showing a huge diversity in morphologic traits and ecologic patterns. In order to improve the knowledge of the karyotype organization of Euphorbia hirta (2n = 18) and E. hyssopifolia (2n = 12), cytogenetic studies were performed by means of conventional staining with Giemsa, genome size estimations with flow cytometry, heterochromatin differentiation with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and Giemsa C-banding, fluorescent in situ hybridization (FISH) with 45S and 5S rDNA probes, and impregnation with silver nitrate (AgNO3). Our results revealed small metacentric chromosomes, CMA+/DAPI0 heterochromatin in the pericentromeric regions of all chromosomes and CMA+/DAPI− in the distal part of chromosome arms carriers of nucleolar organizing regions (NORs). The DNA content measurements revealed small genomes for both species: E. hirta with 2C = 0.77 pg and E. hyssopifolia
Background: Acyclovir (ACV), a synthetic purine nucleoside analogue derived from guanosine, is known to be toxic to gonads and the aim of this study was to evaluate the effect of ACV on the sperm parameters and testosterone production in rat. Materials and Methods: In this experimental study, forty adult male Wistar rats (220 ± 20 g) were randomly divided into five groups (n=8 for each group). One group served as control and one group served as sham control [distilled water was intraperitoneally (i.p.) injected]. ACV was administered intraperitoneally in the drug treatment groups (4, 16 and 48 mg/kg/day) for 15 days. Eighteen days after the last injection, rats were sacrificed by CO2 inhalation. After that, cauda epididymides were removed surgically. At the end, sperm concentrations in the cauda epididymis, sperm motility, morphology, viability, chromatin quality and DNA integrity were analyzed. Serum testosterone concentrations were determined. Results: The results showed that ACV
ID B1VKL9_STRGG Unreviewed; 119 AA. AC B1VKL9; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 18-JUL-2018, entry version 26. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG16864.1}; GN OrderedLocusNames=SGR_35t {ECO:0000313,EMBL:BAG16864.1}, SGR_7104t GN {ECO:0000313,EMBL:BAG23931.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}, and RC NBRC 13350 {ECO:0000313,EMBL:BAG16864.1}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT Genome sequence of the ...
Eukaryotic DNA is packaged in chromatin, whose repeating subunit, the nucleosome, consists of an octamer of histone proteins wrapped by about 147bp of DNA. This packaging affects the accessibility of DNA and hence any process that occurs on DNA, such as replication, repair, and transcription. An early observation from genome-wide nucleosome mapping in yeast was that genes had a surprisingly characteristic structure, which has motivated studies to understand what determines this architecture. Both sequence and trans acting factors are known to influence chromatin packaging, but the relative contributions of cis and trans determinants of nucleosome positioning is debated. Here we present data using genetic approaches to examine the contributions of cis and trans acting factors on nucleosome positioning in budding yeast. We developed the use of yeast artificial chromosomes to exploit quantitative differences in the chromatin structures of different yeast species. This allows us to place approximately 150kb
Hydrocortisone reveals its action by activation of transcription of specific genes that is achieved by binding of hormones nuclear receptors (NRs) to cognate sites on DNA and recruiting coactivators of basal transcription machinery. NRs are able to overcome restricting conformation of chromatin by altering chromatin-remodeling machinery (chromatin-remodeling protein complexes, histone and nonhistone modifying enzymes). However, a possibility arise that genomic DNA accessibility to NRs as well as chromatin packaging pattern in nuclei implicates complex processes which involve regulation of DNA modifying enzymes activities.. Whether hydrocortisone induced decondensation of chromatin alter DNA accessibility in nuclei upon exogenously applied DNAse I and cause eventual changes in endogenous Ca-Mg endonuclease-depended fragmentation become a focus of our research.. Experiments were conducted in vivo. Hydrocortisone 5 mg/100g body wt was administrated by intraperitonial injection. Hormone treated and ...
TY - JOUR. T1 - pRb2/p130 and p107 control cell growth by multiple strategies and in association with different compartments within the nucleus. AU - Zini, Nicoletta. AU - Trimarchi, Carmela. AU - Claudio, Pier Paolo. AU - Stiegler, Peter. AU - Marinelli, Fiorenzo. AU - Maltarello, Maria Cristina. AU - La Sala, Dario. AU - De Falco, Giulia. AU - Russo, Giuseppe. AU - Ammirati, Giuseppe. AU - Maraldi, Nadir Mario. AU - Giordano, Antonio. AU - Cinti, Caterina. PY - 2001. Y1 - 2001. N2 - It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the ...
The repair of DNA damage in highly compact, transcriptionally silent heterochromatin requires that repair and chromatin packaging machineries be tightly coupled and regulated. KAP1 is a heterochromatin protein and co-repressor which binds to HP1 during gene silencing, but is also robustly phosphorylated by ATM at serine 824 in response to DNA damage. The interplay between HP1-KAP1 binding/ATM phosphorylation during DNA repair is not known. We show that HP1α and unmodified KAP1 are enriched in endogenous heterochromatic loci and at a silent transgene prior to damage. Following damage, γH2AX and pKAP1-s824 rapidly increase and persist at these loci. Cells which lack HP1 fail to form discreet pKAP1-s824 foci after damage but levels are higher and more persistent. KAP1 is phosphorylated at Serine 473 in response to DNA damage and its levels are also modulated by HP1. Unlike pKAP1-s824, pKAP1-s473 does not accumulate at damage foci but is diffusely localized in the nucleus. While HP1 association ...
Objective: The objective of this selective EBM review is to determine whether or not dasatinib improves outcomes and tolerability in patients with chronic myeloid leukemia as compared to imatinib. Study Design: Review of three English language, non-blinded randomized controlled trials from 2009, 2010, and 2010. Data Sources: Randomized, controlled, non-blinded clinical trials comparing dasatinib to imatinib or comparing dasatinib once daily vs dasatinib twice daily, found using the PubMed database. Outcomes measured: Overall survival and progression-free survival were measured at one and two years after initiation of therapy. Safety profiles and incidence of adverse effects were also measured. This is graded on a scale of 1 to 4, from lowest in severity to highest in severity. Additionally, adverse effects were noted as hematologic (neutropenia, anemia, thrombocytopenia) or nonhematologic (fluid retention, diarrhea, vomiting, fever). Results: When comparing dasatinib to imatinib, both drugs provided
A new Korn Ferry (NYSE:KFY) survey of Chief Human Resource Officers (CHROs) shows that as the HR function becomes more strategic and high-profile, HR
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Chronic Kidney Disease (CKD) is divided into 5 stages from stage 1 to stage 5. If you have kidney disease, your kidneys are slowly losing their ability to remove wastes and excess water from your blood3 kidney disease indicates moderate chro
In the snowy March of 2003, I climbed Slide Mountain, the tallest of the Catskill range at 4,180 feet, and met a wild-looking man named Sean McFall, who was staying 35 days on Slide s shoulders, in the three-foot snow drifts, with the ice blowing from the treetops and his demonic-looking white bulldog keeping him warm when the temperature dropped to minus 20 degrees Fahrenheit.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
The new REAlease Fluorochrome Technology represents the next step of flexibility in cell sorting. With just one easy step, you can now remove all antibodies from your cells after sorting. | España
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Tekutý kokosový olej na varenie, pečenie, kozmetické účely, antibakteriálny výplach ústnej dutiny, zlepšuje metabolizmus a chudnutie, znižuje cukor
Synonyms for aureolic acid in Free Thesaurus. Antonyms for aureolic acid. 1 synonym for mithramycin: Mithracin. What are synonyms for aureolic acid?
Figure 3 - Phenogram of overall molecular similarity of RAPD bands among specimens from all localities at Tibagi, Paraná, and Iguaçu Rivers. Codes as in Figure 1. DISCUSSION The RAPD-PCR patterns indicate strong genetic differentiation within and among populations in the Paraná and the Tibagi Rivers. In the Paraná River at Porto Rico, the high levels of allelic polymorphism may represent the pristine composition of different species or populations within the H. malabaricus species complex, or they may be the outcome of faunal mixing caused by the closing of the Itaipu Dam in 1978 and the consequent flooding of Sete Quedas, another Oligocenic geographic barrier (Sampaio, 1988). Sete Quedas was the natural boundary for the Upper and Middle Paraná basins. The presence of an outlier in the Paraná River similar to the Iguaçu population suggests that the Paraná may indeed be a recipient of trahiras of diverse origins. The Londrina sample from the Tibagi River is most similar to the populations ...
A method is described in which smears on slides, which had been examined previously in a direct fluorescence antibody (DFA) test for Chlamydia trachomatis, were tested by the polymerase chain reaction (PCR). Twenty four (73%) of 33 smears which contained fewer than 10 elementary bodies when examined by the DFA test were positive by the PCR. Of the nine negative smears, seven contained only one or two elementary bodies. However, single elementary bodies were detected by the PCR in seven of the 24 positive samples. Fifteen smears were negative by both methods. The ability to detect small numbers of elementary bodies by the PCR and its specificity for negative smears indicates its potential for retrospective analysis of stored, archival smears on slides.. ...
ID B1VQ72_STRGG Unreviewed; 299 AA. AC B1VQ72; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 10-APR-2019, entry version 33. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG17173.1}; GN OrderedLocusNames=SGR_344 {ECO:0000313,EMBL:BAG17173.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT Genome sequence of the streptomycin-producing microorganism RT Streptomyces griseus IFO 13350.; RL J. Bacteriol. ...
Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
Eukaryotic organisms package DNA into chromatin for compact storage in the cell nucleus, but this packaging process results in transcriptional repression of genes. Chromatin remodeling complexes have evolved to overcome the transcriptional repression caused by chromatin packaging of DNA into nucleosomes by histones. One example of a chromatin remodeling complex is the SWI/SNF complex in yeast which uses ATP to drive the chromatin apart and make DNA accessible to transcription factors. The yeast SWI2 protein was discovered as the catalytic subunit of the yeast SWI/SNF chromatin remodeling complex and is required for the complex to counteract the repressive nature of chromatin. BRG1 and BRM, SWI2 homologs, are part of human chromatin remodeling complexes and have been shown to play a redundant role in the regulation of certain cell cycle and cellular adhesion genes, as well as cellular pathways. Recent studies showing loss of BRG1 in human tumor cell lines and primary tissue samples, BRG1 ...
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Our group has experience using different techniques for assessing sperm chromatin status in different species (fish, amphibians, mammals). We have used the Comet assay, SCD (Halomax, Halosperm, etc.), SCSA and TUNEL. Currently, we are routinely carrying out SCSA analyses in spermatozoa from several species, including human.. We are open to collaborations with other groups that would be interested in analysing the sperm chromatin but currently do not have the time or resources to carry it out. We are collaborating with several groups that simply store their samples frozen and ship them to our lab from time to time, in order to perform the analyses.. Contact Dr. F. Martínez-Pastor for more information.. ...
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Define panchromatism. panchromatism synonyms, panchromatism pronunciation, panchromatism translation, English dictionary definition of panchromatism. adj. Sensitive to all colors: panchromatic film. pan·chro′ma·tism n. the quality or condition of being lsensitive to all colors, as certain types of...
Myalgic encephalomyelitis (ME) and chronic fatigue syndrome (CFS) affect many thousands of people of all types and ages. Myalgic encephalomyelitis (ME) and chro
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Apparatus and methods for automatically staining or treating multiple tissue samples mounted on slides are provided, in which the slides and reagent bottles are held in fixed position, and the reagent and wash solutions brought to the slides.
Creeping Flows. Steven A. Jones BIEN 501 Wednesday, March 21, 2007 Start on Slide 53. Creeping Flows. Major Learning Objectives: Compare viscous flows to nonviscous flows. Derive the complete solution for creeping flow around a sphere (Stokes flow). Slideshow 4702026 by isaiah
Optional (but a nice supplement to the paper above and also reviews most other species tree methods): Laura Kubatko talked about SVDQuartets in her lecture at the Woods Hole Molecular Evolution Workshop this past summer. Click on the link below, then click on Slides (draft) to download the PDF: the SVDQuartets explanation begins at slide 63. Kubato lecture Symposium talk recordings: ...
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Note on slide reads Birch Bay and Ed took this. Blurred image of people in a body of water. In the foreground are two males, one wearing a hat is bent over at the waist while the other holds on to him and leans on his back. Near the two males is a woman looking toward the camera and holding up the hem of her dress and something white in her hand. Near the woman is a child. In the distance are what could be swimmers and a shoreline ...
Rapid latex test for qualitative & semiquantitative determination of C-reactive protein in serum by agglutination of latex particles on slide read ...
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... chromomycin a3 MeSH D09.408.320.500 - methylgalactosides MeSH D09.408.320.550 - nitrophenylgalactosides MeSH D09.408.320.820 - ...
C19H42BrN Chelerythrine Chromomycin A3 Chaparonin Chitin α-Chloralose Chlorophyll Cholecystokinin (CCK) Cholesterol Choline ...
Kamiyama, M. (May 1968). "Mechanism of action of chromomycin A3. 3. On the binding of chromomycin A3 with DNA and ... Chromomycin A3 binds reversibly to DNA, preferentially to contiguous G/C base pairs. When bound to DNA, Chromomycin A3 has a ... Chromomycin A3 (CMA3) or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of ... Evaluation of male fertility: Chromomycin A3 and protamines compete for the same binding sites in the DNA, so CMA3 positivity ...
Chromomycin A3. *Chaparonin. *Chitin. *α-Chloralose. *Clóraifill b2 Chlorophyll. *Cholecystokinin (CCK). *Colaistéaról ...
Kamiyama, M. (May 1968). "Mechanism of action of chromomycin A3. 3. On the binding of chromomycin A3 with DNA and ... Chromomycin A3 binds reversibly to DNA, preferentially to contiguous G/C base pairs. When bound to DNA, Chromomycin A3 has a ... Chromomycin A3 (CMA3) or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of ... Evaluation of male fertility: Chromomycin A3 and protamines compete for the same binding sites in the DNA, so CMA3 positivity ...
A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic ... NMR STRUCTURE OF D(TTGGCCAA)2 BOUND TO CHROMOMYCIN-A3 AND COBALT. ...
A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic ... AVERAGE SOLUTION STRUCTURE OF D(TTGGCCAA)2 BOUND TO CHROMOMYCIN-A3 AND COBALT. ...
Literature References: Major component of an antitumor antibiotic complex produced by Streptomyces griseus. Binds specifically to guanine-cytosine base pairs in DNA; does not intercalate. The aglycone, chromomycinone, is identical to that of plicamycin, q.v. Isoln of complex and antibacterial activity: M. Shibata et al., J. Antibiot. 13B, 1 (1960), C.A. 54, 22835g (1960). Characterization: K. Mizuno, J. Antibiot. 16A, 22 (1963). Structure: M. Miyamoto et al., Tetrahedron 23, 421 (1967). Abs config: N. Harada et al., J. Am. Chem. Soc. 91, 5896 (1969). Revised structure: J. Thiem, B. Meyer, J. Chem. Soc. Perkin Trans. 2 1979, 1331. Pharmacology and toxicity: M. Slavik, S. K. Carter, Adv. Pharmacol. Chemother. 12, 1 (1975). Fluorescence characteristics: R. H. Jensen, J. Histochem. Cytochem. 25, 573 (1977). Use for analysis and identification of chromosomes: J. H. van de Sande et al., Science 195, 400 (1977); in high-speed chromosome sorting: J. W. Gray et al., ibid. 238, 323 (1987); in flow ...
... ,3D-O-(4-O-acetyl-2,6-dideoxy-3-C-methyl-alpha-L-arabino-hexopyranosyl)-7-methylolivomycin D,aburamycin B, ...
title = "Quantitative footprinting analysis of the chromomycin A3-DNA interaction",. abstract = "Chromomycin A3 (CHR) binding ... N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using ... AB - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using ... Stankus, A, Goodisman, J & Dabrowiak, JC 1992, Quantitative footprinting analysis of the chromomycin A3-DNA interaction, ...
chromomycin A3. DAPI. 4′,6-diamidino-2-phenylindole. FISH. fluorescence in-situ hybridization ...
To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... and A3 (2). 1 and 2 showed potent cytotoxicity against the human gastric adenocarcinoma (AGS) cell line (IC50 1; 1.7 and 2; ... Keywords: chromomycin A2; chromomycin A3; actinomycetes; TRAIL; Wnt chromomycin A2; chromomycin A3; actinomycetes; TRAIL; Wnt ...
chromomycin A3 A glycosidic antineoplastic antibiotic isolated from the bacterium Streptomyces griseus. Chromomycin A3 ... cereblon E3 ubiquitin ligase modulating agent CC-92480 A modulator of the E3 ubiquitin ligase complex containing cereblon (CRL4 ... A modulator of cereblon (CRBN), which is part of the cullin 4-RING E3 ubiquitin ligase complex (CRL4-CRBN E3 ubiquitin ligase; ... Upon administration, cereblon E3 ubiquitin ligase modulating agent CC-92480 specifically binds to cereblon (CRBN), thereby ...
Chromomycin A3. 450. 570. B, BV. G-C Range. 4, 6-Diamidino-2-. Phenylindole HCl (DAPI). 372. 456. U. A-T Range. ...
Crystal structure of the [Co2+-(chromomycin A3)2]-d(CCG)3 complex. ... CoII(Chromomycin)2 Complex Induces a Conformational Change of CCG Repeats from i-Motif to Base-Extruded DNA Duplex. Int J Mol ...
Basic and Clinical Aspects of Sperm Chromomycin A3 Assay Gian Carlo Manicardi, Davide Bizzaro, Denny Sakkas ...
HPLC Application #20027: Multi-Class Screening of 243 Mycotoxins by LC/MS/MS. Column used: Gemini® 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea Part#: 00F-4435-E0
Role of magnesium ion in the interaction between chromomycin A3 and DNA: Binding of chromomycin A3-Mg2+ complexes with DNA. ... Chromomycin A3(Chro). The anticancer antibiotic chromomycin A3 (Chro), a DNA minor groove binding drug which interferes with ... Slavik, M.; Carter, S.K. Chromomycin A3, mithramycin, and olivomycin: Antitumor antibiotics of related structure. Adv. ... chromomycin A3 with DNA: Does neutral antibiotic bind DNA in absence of the metal ion? J. Biomol. Struct. Dyn 2000, 18, 209-218 ...
Six compounds (UOK257-FLCN+-inhibitory: cyanomorpholino-ADR, bruceantin, chromomycin A3 (ChA3); and UOK257-FLCN−-inhibitory: ... Morpholino-ADR (NSC 354646), cyanomorpholino-ADR (NSC 357704), echinomycin (NSC 13502), chromomycin A3 (NSC 58514), bruceantin ...
NMR studies of chromomycin A3 interaction with DNA. Biochemistry. 1985 Nov 19; 24(24):6887-93. ...
... chromomycin A3 and silver staining ... Chromomycin A3 stains nucleolar organizer regions of fish ...
Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol ... discrepancy between chromomycin and aniline blue staining. Reprod Biomed Online. (2009) 19:264-9. doi: 10.1016/S1472-6483(10) ...
A method for determining the chirality of two aromatic chromophores and the absolute configurations of chromomycin A3 and ... The four chromoses from chromomycin A3 Tetrahedron Letters. 5: 2371-2377. 1. ... Chromomycinone, the aglycone of chromomycin A3 Tetrahedron Letters. 5: 2355-2365. 1. ...
Of those, chromomycin-a3, fulvestrant and apicidin were active in nanomolar doses in all the three strains tested, with the ... Chromomycin-a3 (AC50 = 0.011 µM), fulvestrant (0.0667 µM), apicidin (0.078 µM), ingenol (1.4408 µM) and tacrolimus (2.4383 µM) ... In this study, the highest active single agents were apicidin, fulvestrant and chromomycin-a3. The only single agent that is ... The results of the screen are summarised in Table 1, where chromomycin-a3 (CHR), fulvestrant (FUL), apicidin (API), ingenol ( ...
本研究題目主要是以抗癌藥與目標物的結合機制及其在癌症疾病的治療作一系列的探討。抗生素Mithramycin 和Chromomycin A3 是一種鍵結於DNA的aureolic 類抗癌藥物。 其活性主要來自於干擾DNA的複製及轉錄作用,並運用於臨床的癌症 ... Chen, Y.W. and Hou,M.H.(2013) The binding of the Co(II) complex of dimeric chromomycin A3 to GC sites with flanking G:G ... Chen, Y.W. and Hou,M.H.(2013). The binding of the Co(II) complex of dimeric chromomycin A3
Silva DJ, Goodnow R, Kahne D. The sugars in chromomycin A3 stabilize the Mg(2+)-dimer complex. Biochemistry. 1993 Jan 19; 32(2 ... design and synthesis of a ligand based on chromomycin A3. Bioorg Med Chem. 1994 Nov; 2(11):1251-9. PMID: 7757421. ...
Fluorescence R-banding with chromomycin A3 and methyl green was performed as described in detail earlier (53). Whenever ...
Chromomycin A3 (CMA3) is a fluorescent dye that intercalates into DNA but because of its size it can only bind to sperm DNA ... 2009). Use of chromomycin A3 staining in bovine sperm cells for detection of protamine deficiency. Biotech. Histochem. 84, 79- ... chromomycin A3 (CMA3) staining, and for isolation of protein for AU-PAGE, or were frozen. ...
Normal sperm morphology and chromatin packaging: Comparison between aniline blue and chromomycin A3 staining Franken D.R.; ...
... chromomycin A3 and Aniline blue staining).. RESULTS: After 35 days, in addition to above mentioned sperm parameters, all of the ...
Two helium-cadmium (HeCd) lasers, emitting 16 mW at 442 nm and 35 mW at 325 nm, were used to excite chromomycin A3 ... ... The analysis of chromosomes was performed through Ag-staining, C-banding, chromomycin A3 and DAPI staining, and fluorescent in ... In the present investigation chromosomal preparations of Asellus aquaticus were sequentially stained with chromomycin A3 to ... Quinacrine and distamycin-DAPI, which selectively stain AT-rich DNA, and chromomycin, specific for GC-rich sequences, were used ...
... monobromobimane and chromomycin A3). Principal component analysis (PCA) was used to identify the parameters that most ...
DNA fragmentation and semen parameters were assessed by chromomycin A3, TUNEL staining and WHO criteria respectively. Total ...
... chromomycin A3, epilubisin hydrochloride, ansamycin, carzinophilin and pyralvicin; plant alkaloids such as vinblastine sulfate ...
  • Chromomycin A3 (CMA3) or Toyomycin is an anthraquinone antibiotic glycoside produced by the fermentation of a certain strain of Streptomyces griseus (No. 7). (wikipedia.org)
  • Evaluation of male fertility: Chromomycin A3 and protamines compete for the same binding sites in the DNA, so CMA3 positivity in spermatozoa reflects protamine deficiency (affecting sperm morphology and decreasing fertility). (wikipedia.org)
  • Protamine deficiency and the percentage of apoptotic spermatozoa were evaluated using chromomycin A3 (CMA3) staining and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assays, respectively. (ecerm.org)
  • Além do conteúdo absoluto de DNA, a composição de bases também foi mensurada para as duas espécies utilizando núcleos corados com 4 ,6 -diamidino- 2-fenilindole (DAPI) e cromomicina A3 (CMA3). (ibict.br)
  • Chromomycin A3 (CMA3) fluorescent staining (green) and DAPI (blue) staining in C. albula (a) and C. fontanae (b). (nih.gov)
  • Protamine deficiency and the proportion of apoptotic spermatozoa were evaluated using chromomycin A3 (CMA3), aniline blue (AB), and toluidine blue (TB) staining, as well as the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. (bvsalud.org)
  • Chromomycin A3 (CMA3) staining, either by the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. (ijfs.ir)
  • Protamine efficiency can be assessed indirectly by chromomycin A3 (CMA3), which has been shown to compete with protamine to bind DNA ( 7 - 9 ). (ijfs.ir)
  • Chromomycin A3 (CMA3) staining was used to determine protamine deficiency. (storysteel.gq)
  • Ejaculates were analyzed and chromatin quality was assessed using chromomycin A3 (CMA3) staining. (jri.ir)
  • Metaphase chromosomes were stained with the fluorescent dyes Hoechst 33258 and Chromomycin A3 and analyzed subsequently using bivariate flow cytometry. (eurekamag.com)
  • Dual-beam high-speed sorting has been developed to facilitate purification of chromosomes based on DNA staining with the fluorescent dyes Hoechst 33258 and chromomycin A3. (elsevier.com)
  • For bivariate flow karyotyping, the DNA composition of specific chromosomes in suspension is measured based on the DNA-specific dyes Hoechst 33258 and chromomycin A3 (3,4). (scienceexchange.com)
  • The analysis of chromosomes was performed through Ag-staining, C-banding, chromomycin A3 and DAPI staining, and fluorescent in situ hybridization with ribosomal genes. (biomedsearch.com)
  • Metaphase plates and karyograms of C. albula and C. fontanae showing Chromomycin A3/DAPI staining, FISH and CGH experiments. (nih.gov)
  • Sperm samples were analyzed for parameters (motility, morphology and count), DNA integrity (SCD test) and chromatin condensation (chromomycin A3 and Aniline blue staining). (greenmedinfo.com)
  • Sperm DNA integrity was analyzed through fragmentation susceptibility (toluidine blue staining and Sperm Chromatic Structure Assay - SCSA), direct evaluation of DNA fragmentation (Sperm Chromatin Dispersion Assay - SCDA) and sperm protamination (chromomycin A3). (biomedcentral.com)
  • Sperm chromatin assay was assessed by cytochemical tests including aniline blue, chromomycin A3, and toluidine blue. (who.int)
  • Then the parameters and chromatin integrity of the epididymal sperm were analyzed using chromomycin A3, toluidine blue (TB), and acridine orange (AO) staining. (bvsalud.org)
  • Sperm DNA integrity was analysed by flow cytometry using three fluorescent probes (acridine orange, monobromobimane and chromomycin A3). (diva-portal.org)
  • Preparations of semen were stained with acridine orange, aniline blue, and chromomycin A3. (edu.pl)
  • Chromomycin, mithramycin, and olivomycin binding sites on heterogeneous deoxyribonucleic acid. (wikipedia.org)
  • Anti-cancer antibiotics, chromomycin A3 (CHR) and mithramycin (MTR) inhibit DNA directed RNA synthesis in vivo by binding reversibly to template DNA in the minor groove with GC base specificity, in the presence of divalent cations like Mg2+. (bvsalud.org)
  • The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. (bvsalud.org)
  • Cependant, les chiens avec HBP ont une susceptibilité plus élevée à la dénaturation acide de l'ADN des spermatozoïdes (SCSA) par comparaison aux chiens ne présentant pas d'HBP, ainsi qu'un pourcentage plus bas de spermatozoïdes avec intégrité de l'ADN (coloration au bleu de toluidine). (biomedcentral.com)
  • détermination de la structure de la chromatine des spermatozoïdes - SCSA), par l'évaluation directe de la fragmentation de l'ADN des spermatozoïdes (détermination de la dispersion de la chromatine des spermatozoïdes - SCDA) et par l'évaluation de la protamination des spermatozoïdes (chromomycine A3). (biomedcentral.com)
  • [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (go.jp)
  • a) Five antibiotics known to be produced by S. griseus -the aminoglycoside streptomycin and three polyketides: daunomycin, fredericamycin A, chromomycin A3, and a hybrid nonribosomal peptide-polyketide tetramate macrolactam. (asmscience.org)
  • Protamine deficiency, DNA fragmentation and semen parameters were assessed by chromomycin A3, TUNEL staining and WHO criteria respectively. (sid.ir)
  • abstract = "Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. (syr.edu)
  • Dans la présente étude, 20 chiens mâles ont été randomisés selon un plan factoriel en 2x2 à l'un des 4 groupes expérimentaux suivants : Groupe HBP (n=5), Groupe HBP-Finastéride (n=5), Groupe non-HBP traité par Finastéride (n=5), et Groupe non-HBP non traité (n=5). (biomedcentral.com)
  • The results evidenced that this compound matches the biological properties of Chromomycin A3 and Actinomycin D, known drugs with high DNA binding affinity. (unipa.it)
  • In the presence of Mg2+ ions, Chromomycin A3 binds reversibly to DNA, preferentially to contiguous G/C base pairs. (wikipedia.org)
  • When bound to DNA, Chromomycin A3 has a maximum excitation wavelength of 445 nm (blue), and a maximum emission wavelength of 575 nm (yellow). (wikipedia.org)
  • Chen, Y.W. and Hou,M.H. (2013) The binding of the Co(II) complex of dimeric chromomycin A3 to GC sites with flanking G:G mismatches. (nchu.edu.tw)
  • The sugars in chromomycin A3 stabilize the Mg(2+)-dimer complex. (naver.com)