A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria occurring in soil and water. Its organisms are generally nonpathogenic, but some species do cause infections of mammals, including humans.
Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.
Discarded electronic devices containing valuable and sometimes hazardous materials such as LEAD, NICKEL, CADMIUM, and MERCURY. (from http://www.epa.gov/osw/conserve/materials/ecycling/faq.htm#impact accessed 4/25/2010)
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.

Effective method for activity assay of lipase from Chromobacterium viscosum. (1/183)

A method was devised for activity assay of the lipase [triacylglycerol acyl-hydrolase, EC 3.1.1.3] excreted from Chromobacterium viscosum into the culture medium; olive oil emulsified with the aid of Adekatol 45-S-8 (a non-ionic detergent, the ethoxylate of linear sec-alcohols having chain lengths of 10--16 carbon atoms) was used as the substrate. This method was specifically effective for Chromobacterium lipase acitvity assay, and was approximately twice as sensitive as the conventional method, in which polyvinyl alcohol is used for the emulsification of the substrate.  (+info)

Two cases of Chromobacterium violaceum infection after injury in a subtropical region. (2/183)

Chromobacterium violaceum is a gram-negative rod and is isolated from soil and water in tropical and subtropical regions. The species have pigmented and nonpigmented colony types. Infections caused by nonpigmented strains are rare. We report on two cases of infection caused by both pigmented and nonpigmented strains of C. violaceum. Two 24-year-old Korea Airline stewardesses were admitted to Inha University Hospital, Inchon, South Korea, on 9 August 1997, 3 days after an airplane accident in Guam. Both had multiple lacerations on exposed parts of their bodies. There was swelling, tenderness, and pus discharge. The wounds contained many small fragments of stones and weeds. A pigmented strain was isolated from the left hand and a nonpigmented strain was isolated from the left knee of one patient. For the other patient only a nonpigmented strain was isolated from a foot wound. The nonpigmented colonies from the left-knee and the left-foot wounds did not produce any pigment even after an extended period of incubation. The biochemical characteristics were the same for each strain except for oxidase and indole reactions. The pigmented strain was oxidase negative and indole positive, whereas the nonpigmented strains were oxidase positive and indole negative. The patients were successfully treated by debridement and with appropriate antibiotics.  (+info)

Molecular dynamics of microbial lipases as determined from their intrinsic tryptophan fluorescence. (3/183)

We have studied the intrinsic tryptophan fluorescence of the lipases from Chromobacterium viscosum (CVL), Pseudomonas species (PSL), and Rhizopus oryzae (ROL) in aqueous buffer, zwitterionic detergent micelles, and isopropanol-water mixtures. It was the purpose of this study to obtain information about biophysical properties of the respective enzymes under conditions that modulate enzyme activities and stereoselectivities to a significant extent. According to their decay-associated emission spectra, CVL tryptophans are located in the hydrophobic interior of the protein. In contrast, the PSL and ROL tryptophans are probably confined to the core and the surface of the lipase. From the tryptophan lifetime distributions it can be concluded that the conformation of CVL is not much affected by detergent or organic solvent (isopropanol). Accordingly, CVL is enzymatically active in these systems and most active in the presence of isopropanol. In contrast, ROL and PSL show high conformational mobility, depending on the solvent, because their lifetime distributions are very different in the presence and absence of detergent or isopropanol. Time-resolved anisotropy studies provided evidence that the lipases exhibit very high internal molecular flexibility. This peculiar feature of lipases is perhaps the key to the great differences in activity and stereoselectivity observed in different reaction media. Furthermore, information about self-association of the lipases in different solvents could be obtained. PSL, but not CVL and ROL, forms aggregates in water. Lipase aggregation can be reversed by the addition of detergent or isopropanol, which competes for the hydrophobic surface domains of this protein. This dissociation could efficiently contribute to the increase in lipase activity in the presence of a detergent or isopropanol.  (+info)

Cloning, molecular analysis, and expression of the polyhydroxyalkanoic acid synthase (phaC) gene from Chromobacterium violaceum. (4/183)

The polyhydroxyalkanoic acid synthase gene from Chromobacterium violaceum (phaC(Cv)) was cloned and characterized. A 6.3-kb BamHI fragment was found to contain both phaC(Cv) and the polyhydroxyalkanoic acid (PHA)-specific 3-ketothiolase (phaA(Cv)). Escherichia coli strains harboring this fragment produced significant levels of PHA synthase and 3-ketothiolase, as judged by their activities. While C. violaceum accumulated poly(3-hydroxybutyrate) or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) when grown on a fatty acid carbon source, Klebsiella aerogenes and Ralstonia eutropha (formerly Alcaligenes eutrophus), harboring phaC(Cv), accumulated the above-mentioned polymers and, additionally, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) when even-chain-length fatty acids were utilized as the carbon source. This finding suggests that the metabolic environments of these organisms are sufficiently different to alter the product range of the C. violaceum PHA synthase. Neither recombinant E. coli nor recombinant Pseudomonas putida harboring phaC(Cv) accumulated significant levels of PHA. Sequence analysis of the phaC(Cv) product shows homology with several PHA synthases, most notably a 48% identity with that of Alcaligenes latus (GenBank accession no. AAD10274).  (+info)

Chromobacteriosis in a Chinese red panda (Ailurus fulgens styani). (5/183)

An adult Chinese red panda (Ailurus fulgens styani) transported by airplane from Florida to a North Dakota zoo died 1 week after arrival. Grossly, an interscapular abscess, subcutaneous inflammation, lymphadenitis, and pulmonary abscesses were observed. Microscopic findings included necrotizing inflammation in liver, lung, lymph node, and spleen. Chromobacterium violaceum was cultured from the interscapular abscess, liver, lung, and spleen and was injected into Swiss Webster mice. These mice died 18 hours postinoculation, and C. violaceum was cultured from liver, lung, and spleen. Chromobacterium violaceum is a sporadically reported but highly virulent pathogenic bacterium of both animals and humans typically found as a soil and water inhabitant of tropical and subtropical regions.  (+info)

Intrinsic conformation of lipid A is responsible for agonistic and antagonistic activity. (6/183)

Lipopolysaccharides (LPS, endotoxin) represent a major virulence factor of Gram-negative bacteria, which can cause septic shock in mammals, including man. The lipid anchor of LPS to the bacterial outer membrane, lipid A, exhibits a peculiar chemical structure, harbours the 'endotoxic principle' of LPS and is also responsible for the expression of pathophysiological effects. Chemically modified lipid A can be endotoxically inactive, but may express strong antagonistic activity against endotoxically active LPS. By applying orientation measurements with attenuated total reflectance (ATR) infrared spectroscopy on hydrated lipid A samples, we show here that these different biological activities are directly correlated to the intrinsic conformation of lipid A. Bisphosphoryl-hexaacyl lipid A molecules with an asymmetric (4/2) distribution of the acyl chains linked to the diglucosamine backbone have a large tilt angle (> 45 degrees ) of the diglucosamine backbone with respect to the membrane surface, a conical molecular shape (larger cross-section of the hydrophobic than the hydrophilic moiety), and are endotoxically highly active. Monophosphoryl hexaacyl lipid A has a smaller tilt angle, and the conical shape is less expressed in favour of a more cylindrical shape. This correlates with decreasing endotoxic activity. Penta- and tetraacyl lipid A or hexaacyl lipid A with a symmetric acyl chain distribution (3/3) have a small tilt angle (< 25 degrees ) and a cylindrical shape and are endotoxically inactive, but may be antagonistic.  (+info)

Chromobacterium violaceum infection in Brazil. A case report. (7/183)

We report the second case of infection with Chromobacterium violaceum that occurred in Brazil. A farm worker living in the State of Sao Paulo presented fever and severe abdominal pain for four days. At hospitalization the patient was in a toxemic state and had a distended and painful abdomen. Chest X-ray and abdominal ultrasound revealed bilateral pneumonia and hypoechoic areas in the liver. The patient developed failure of multiple organs and died a few hours later. Blood culture led to isolation of C. violaceum resistant to ampicillin and cephalosporins and sensitive to chloramphenicol, tetracyclin, aminoglicosydes, and ciprofloxacin. Autopsy revealed pulmonary microabscesses and multiple abscesses in the liver. The major features of this case are generally observed in infections by C. violaceum: rapid clinical course, multiple visceral abscesses, and high mortality. Because of the antimicrobial resistance profile of this Gram-negative bacillus, for appropriate empirical antibiotic therapy it is important to consider chromobacteriosis in the differential diagnosis of severe community infections in Brazil.  (+info)

Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. (8/183)

In gram-negative bacteria, many important changes in gene expression and behavior are regulated in a population density-dependent fashion by N-acyl homoserine lactone (AHL) signal molecules. Exudates from pea (Pisum sativum) seedlings were found to contain several separable activities that mimicked AHL signals in well-characterized bacterial reporter strains, stimulating AHL-regulated behaviors in some strains while inhibiting such behaviors in others. The chemical nature of the active mimic compounds is currently unknown, but all extracted differently into organic solvents than common bacterial AHLs. Various species of higher plants in addition to pea were found to secrete AHL mimic activities. The AHL signal-mimic compounds could prove to be important in determining the outcome of interactions between higher plants and a diversity of pathogenic, symbiotic, and saprophytic bacteria.  (+info)

TY - JOUR. T1 - Mechanism of Metal-Independent Hydroxylation by Chromobacterium violaceum Phenylalanine Hydroxylase. AU - Carr, Robert T.. AU - Balasubramanian, Shankar. AU - Hawkins, Paul C.D.. AU - Benkovic, Stephen. PY - 1995/1/1. Y1 - 1995/1/1. N2 - Phenylalanine hydroxylase converts phenylalanine to tyrosine utilizing a tetrahydrobiopterin cofactor. Several key mechanistic questions have yet to be resolved, specifically the identity of the hydroxylating species and the role of the non-heme iron which is present in all of the mammalian PAHs. Recently, we have demonstrated that a bacterial PAH from Chromobacterium violaceum does not require any redox active metal for activity [Carr, R. T., & Benkovic, S. J. (1993) Biochemistry 32, 14132-14138]. To identify the function of iron in the mammalian PAHs, we have undertaken a series of experiments to compare the mechanisms of this metal-independent PAH with the iron-dependent PAH from rat liver. Using [4-2H]phenylalanine as a substrate gave a ...
TY - JOUR. T1 - X-ray Absorption Studies of the Cu-Dependent Phenylalanine Hydroxylase from Chromobacterium violaceum. Comparison of the Copper Coordination in Oxidized and Dithionite-Reduced Enzymes. AU - Blackburn, Ninian J.. AU - Strange, Richard W.. AU - Carr, Robert T.. AU - Benkovic, Stephen J.. PY - 1992/2/1. Y1 - 1992/2/1. N2 - The coordination chemistry of the Cu sites of phenylalanine hydroxylase (PAH) from Chromobacterium violaceum has been studied by X-ray absorption spectroscopy (XAS). The EXAFS of the Cu(II) form of the enzyme resembles that of other non-blue copper proteins such as plasma amine oxidases and dopamines-hydroxylase and is characteristic of a mixed N/O coordination shell containing histidine ligation. Detailed simulations of the raw EXAFS data have been carried out using full curved-wave restrained refinement methodologies which allow imidazole ligands to be treated as structural units. The results suggest a Cu(II) coordination of two histidines and two additional ...
Chromobacterium violaceum is a Gram-negative, facultative anaerobic, non-sporing coccobacillus. It is motile with the help of a single flagellum which is located at the pole of the coccobacillus. Usually, there are one or two more lateral flagella as well. It is part of the normal flora of water and soil of tropical and sub-tropical regions of the world. It produces a natural antibiotic called violacein, which may be useful for the treatment of colon and other cancers. It grows readily on nutrient agar, producing distinctive smooth low convex colonies with a dark violet metallic sheen (due to violacein production). Some strains of the bacteria which do not produce this pigment have also been reported. It has the ability to break down tarballs. C. violaceum ferments glucose, trehalose, N-acetylglucosamine and gluconate but not L-arabinose, D-galactose, or D-maltose. It is positive for catalase and oxidase reactions. Bacterial isolates in many cases can show high level resistance to a range of ...
article{2f5ed112-7711-4829-944a-2b87a0437947, abstract = {SUMMARY: The bacterial ω-transaminase from Chromobacterium violaceum (Cv-ωTA, EC 2.6.1.18) catalyzes industrially important transamination reactions by use of the coenzyme pyridoxal 5-phosphate (PLP). Here, we present four crystal structures of Cv-ωTA: two in the apo form, one in the holo form and one in an intermediate state, at resolutions between 1.35 and 2.4 Å. The enzyme is a homodimer with a molecular weight of approximately 100 kDa. Each monomer has an active site at the dimeric interface that involves amino acid residues from both subunits. The apo-Cv-ωTA structure reveals unique relaxed conformations of three critical loops involved in structuring the active site, that have not previously been seen in a transaminase. Analysis of the four crystal structures reveals major structural rearrangements involving elements of the large and small domains of both monomers that reorganize the active site in the presence of PLP. The ...
Violacein, a pigment isolated from Chromobacterium violaceum, has been reported to have multiple biological activities including in vitro antitumor effects. Certain anticancer agents are known to induce apoptosis in human tumor cell lines. In this work, our aim was to investigate the effectiveness of violacein/β-cyclodextrin (β-CD)-containing systems to produce lethal effects in the human promyelocytic leukemia cell line HL60. Using the MTT tetrazolium reduction test, IC 50 for the inclusion complexes (1:1 and 1:2 violacein:β-CD molar ratios) and violacein alone were less than 1 μM. Violacein and violacein/β-CD complexes were able to induce NBT reduction. Moreover, by using the Feulgen reaction, all the compounds were found to trigger apoptosis in HL60 cells, inducing around 35% of DNA fragmentation, as analyzed through the diphenylamine assay. In addition, caspases seem to play an important role in the activation of the executioner phase of apoptosis induced by violacein and its ...
0129] Asolkar, R. N., Jensen, P. R., Kauffman, C. A., Fenical, W. 2006. Daryamides A-C, Weakly Cytotoxic Polyketides from a Marine-Derived Actinomycete of the Genus Streptomyces strain CNQ-085 J. Nat. Prod. 69:1756-1759. [0130] Arena, J. P., K. K. Liu, et al. (1995). The mechanism of action of avermectins in Caenorhabditis elegans--correlation between activation of glutamate-sensitive chloride current, membrane binding and biological activity. Journal of Parasitology 81: 286-294. [0131] Balibar, C. J. and C. T. Welsh (2006). In Vitro Biosynthesis of Violacein from L-Tryptophan by the Enzymes VioA-E from Chromobacterium violaceum. Biochemistry 45: 15444-15457. [0132] Chalvet-Monfray, K., P. Sabatier, et al. (1996). Synergy between deltamethrin and prochloraz in bees: Modeling approach. Environmental Toxicology and Chemistry 15(4): 525-534. [0133] Chitwood, D. J. (2003). Nematicides. Encyclopedia of Agrochemicals, vol 3. J. R. Plimmer. New York, John Wiley & Sons. 3: 1104-1115. [0134] Colby, ...
1LTV: Structural comparison of bacterial and human iron-dependent phenylalanine hydroxylases: similar fold, different stability and reaction rates.
1LTV: Structural comparison of bacterial and human iron-dependent phenylalanine hydroxylases: similar fold, different stability and reaction rates.
The increasing incidence of multidrug-resistant pathogens has stimulated the search for novel anti-virulence compounds. Although many phytochemicals show promising antimicrobial activity, their power lies in their anti-virulence properties. Thus the quorum sensing (QS) inhibitory activity of four crude Kigelia africana fruit extracts was assessed qualitatively and quantitatively using the Chromobacterium violaceum and Agrobacterium tumefaciens biosensor systems. Inhibition of QS-controlled violacein production in C. violaceum was assayed using the qualitative agar diffusion assay as well as by quantifying violacein inhibition using K. africana extracts ranging from 0.31-8.2 mg/mL. Qualitative modulation of QS activity was investigated using the agar diffusion double ring assay. All four extracts showed varying levels of anti-QS activity with zones of violacein inhibition ranging from 9-10 mm. The effect on violacein inhibition was significant in the following order: hexane > dichloromethane >
A series of selected 2-substituted imidazolines were synthesized in moderate to excellent yields by a modification of protocols reported in the literature. They were evaluated as potential non-classical bioisosteres of AHL with the aim of counteracting bacterial pathogenicity. Imidazolines 18a, 18e and 18f at various concentrations reduced the violacein production by Chromobacterium violaceum, suggesting an anti-quorum sensing profile against Gram-negative bacteria. Imidazoline 18b did not affect the production of violacein, but had a bacteriostatic effect at 100 µM and a bactericidal effect at 1 mM. Imidazoline 18a bearing a hexyl phenoxy moiety was the most active compound of the series, rendering a 72% inhibitory effect of quorum sensing at 100 µM. Imidazoline 18f bearing a phenyl nonamide substituent presented an inhibitory effect on quorum sensing at a very low concentration (1 nM), with a reduction percentage of 28%. This compound showed an irregular performance, decreasing inhibition at
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TY - JOUR. T1 - Extracts of macroalgae from the Brazilian coast inhibit bacterial quorum sensing. AU - Batista, Daniela. AU - Carvalho, Ana Polycarpa. AU - Costa, Rafaela. AU - Coutinho, Ricardo. AU - Dobretsov, Sergey. PY - 2014/12/1. Y1 - 2014/12/1. N2 - The ability of polar (1:1 water/methanol) and non-polar (dichloromethane) extracts of macroalgae from Arraial do Cabo, RJ, Brazil, to inhibit quorum sensing (QS) was investigated. Before extraction, half of the algae were treated with 30% ethanol to kill and remove surface-associated bacteria. Twenty of 22 polar extracts inhibited the QS of the acyl homoserine lactone (AHL) producer and reporter Chromobacterium violaceum CV017 with minimal inhibitory concentrations (MIC) ranging from 0.28 μg ml-1 (Ulva fasciata) to 189 μg ml-1 (Codium sp.). The MIC of non-polar extracts varied from 69 μg ml-1 (Sargassum furcatum) to 2730 μg ml-1 (Peyssonnelia capensis). Usually, extracts from algae with associated bacteria had a higher bioactivity than ...
With the rise in antibiotic resistance, quorum sensing inhibition has emerged as a possible alternative to classical antibiotic therapy. Quorum sensing is density-dependent communication between bacteria that allows the bacteria to coordinate gene expression. This communication is accomplished through the secretion of certain signal molecules, and is often responsible for the development of pathogenicity in bacteria. By inhibiting this communication, bacteria may be prevented from expressing pathogenic traits, such as biofilm formation. In this study, 12 novel Pyrrolo[2,1-c][1,4]benzodiazepine (PBD) compounds were tested for their ability to inhibit quorum sensing using disk diffusion assays. Blank disks were loaded with the PBD compounds and overlaid with molten agar inoculated with Chromobacterium violaceum. C. violaceum is a quorum sensing indicator species. The bacteria produce a purple pigment when they undergo quorum sensing but turn white when quorum sensing is inhibited. Compounds that showed
FK228 (formerly FR901228) was recently isolated from Chromobacterium violaceum as a potent antitumor agent and its biologic target protein was identified as histone deacetylase (HDAC). Because of its unique chemical structure (i.e., bicyclic depsipeptide) and activity profile in the National Cancer …
Pictured is Chromobacterium violaceum and Staphylococcus aureus shaped in a valentine-themed drawing. This drawing was done at a microbiology lab at Cepheid, Sunnyvale, CA. C. violaceum is a gram-negative, motile bacillus. It produces dark violet colonies due to the bacteriums natural production of violacein, which is a pigmented antibiotic. This organism is commonly found in water and soil from tropical and subtropical areas of the world ...
Programmed cell death is a powerful anti-microbial defense. The innate immune system is our first line of defense against infection, and the induction of cell death can rapidly remove intracellular niches. Inflammasomes are multi-protein signaling complexes that result in pyroptosis, a form of lytic programmed cell death. However, a lack of strong in vivo inflammasome phenotypes has led to the conclusion that inflammasomes merely slow the infection until the adaptive immune system is activated. In Chapter 2, we report that inflammasomes provide penetrating and sterilizing immunity against two ubiquitous environmental bacteria, Chromobacterium violaceum and Burkholderia thailandensis. Additionally, the study of C. violaceum revealed the first evidence of natural killer (NK) cell cytotoxicity in defense against a bacterium in vivo. Thus, environmental microbes are powerful immunological probes and have great potential as model organisms. NK cells and cytotoxic T lymphocytes (CTLs) canonically ...
Business Directory for Violet Pigment Suppliers in Ahmedabad - Get contact details of Violet Pigment Manufacturers, Wholesale Violet Pigment Exporters, Best Violet Pigment Traders & Distributors Across the Ahmedabad.
A novel method for the assay of polyhydroxyalkanoate (PHA)-degrading ability of triacylglycerol lipases was developed. By applying the natural affinity of lipases towards hydrophobic interfaces, a sensitive and rapid densitometry analysis for the evaluation of hydrolytic activity of lipase droplets towards PHA-coated surface was successfully carried out. We found that 12 out of 14 tested lipases which are of fungal, bacterial and animal origin were able to hydrolyze P(3HB-co-92 mol% 4HB) thin film. The patterns and opacity of the hydrolysis spots of lipases on PHA films allowed easy comparison of PHA-hydrolytic strength of lipases. Lipase from the bacterium Chromobacterium viscosum exhibited the highest PHA-degrading activity. The hydrolytic activity of lipases on water insoluble PHA, emulsified p-nitrophenyl laurate and olive oil were also compared and interestingly some lipases showed better activity when PHA was used as a substrate.
The Marcgraviaceae is a neotropical plant family of lianas and shrubs distributed throughout Central and South America, mainly in evergreen and semi-evergreen tropical forests. In this study, extracts of 12 Marcgraviaceae species were assessed for inhibition of bacterial quorum sensing (QS) in Chromobacterium violaceum, bacterial biofilm formation in Pseudomonas aeruginosa PA14, and fungal growth in Saccharomyces cerevisiae. Six species showed promising inhibitory activities in at least one of the three bioassays (Marcgravia nervosa Triana & Planch., Marcgravia polyantha Delp., Marcgravia schippii Standl., Marcgraviastrum subsessile (Benth.) Bedell, Schwartzia brasiliensis (Choisy) Bedell ex Gir-Cañas, and Schwartzia costaricensis (Gilg.) Bedell). Analyses of the crude extract of the leaves of Marcgravia nervosa using HPLC-APCI-MS showed the presence of five pentacyclic triterpenes: ursolic acid (2), betulinic acid (3), α-amyrin (4), β-amyrin (5), lupeol (6). Bioassay-guided fractionation of ...
Safety in the scientific environment is an important issue. To begin with, organisms subject to research as well as substances and chemicals used may be hazardous. Since synthetic biology uses modified organisms it is also important to consider possible effects on ecosystems. But safety is not limited to organisms and chemicals. Stress and fatigue of the researchers involved must also be taken into account when designing experiments. To address these issues we reviewed available safety protocols and regulations and wrote new protocols to fit our needs. At the beginning of the project, Dr. Gunaratna Kuttuva Rajarao gave instructions on lab safety basics and sterile techniques. In general, we followed the safety guidelines of KTH, Royal Institute of Technology for this project.. Throughout this project, we used E.coli (TOP10, BW25113) which falls under biosafety level 1. Moreover, Chromobacterium violaceum was used as a readout strain in an experiment that falls under biosafety level 2. We ...
Romidepsin was first reported in the scientific literature in 1994, by a team of researchers from Fujisawa Pharmaceutical Company (now Astellas Pharma) in Tsukuba, Japan, who isolated it in a culture of Chromobacterium violaceum from a soil sample obtained in Yamagata Prefecture.[3] It was found to have little to no antibacterial activity, but was potently cytotoxic against several human cancer cell lines, with no effect on normal cells; studies on mice later found it to have antitumor activity in vivo as well.[3]. The first total synthesis of romidepsin was accomplished by Harvard researchers and published in 1996.[4] Its mechanism of action was elucidated in 1998, when researchers from Fujisawa and the University of Tokyo found it to be a histone deacetylase inhibitor with effects similar to those of trichostatin A.[5]. ...
The biosynthetic pigment from Chromobacterium violaceum BB-78, 1,3-dihydro-2H-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. A relatively high cytotoxicity on V-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. The methylol derivative exhibited similar toxicity as Nifurtimox, a known, commercial trypanocide compound ...
Description: A monocyclic beta-lactam antibiotic originally isolated from Chromobacterium violaceum. It is resistant to beta-lactamases and is used in gram-negative infections, especially of the meninges, bladder, and kidneys. It may cause a superinfection with gram-positive organisms ...
In comparison to the adult birds, young chickens and turkeys have a reduced ability to use dietary fat. It was of interest to investigate the role of lipase in fat malabsorption in young birds. It was hypothesized that fat malabsorption in young chickens and turkeys is due to an insufficiency of pancreatic lipase. It was further hypothesized that fat utilization in young birds can be improved by the use of dietary lipase sources. The first in vitro experiment characterized the activity and stability of mammalian, fungal and bacterial lipase sources when exposed to conditions associated with the proventriculus of young birds. The results of this study demonstrated that bacterial Pseudomonas sp. and Chromobacterium viscosum lipase sources are more stable under conditions that mimic the glandular stomach of young birds. Aspergillus niger lipase also showed a relatively high activity in acidic conditions. Mammalian crude porcine lipase was irreversibly inhibited in these conditions. The second in ...
Phragmidium violaceum[4] maoy kaliwatan sa uhong kay sakop sa division nga Basidiomycota, ug nga una nga gihulagway ni Schultz, ug nga gihatagan sa eksakto nga ngalan ni Georg Winter ni adtong 1880. Ang Phragmidium violaceum kay sakop sa henero nga Phragmidium, ug pamilya nga Phragmidiaceae.[5][6] Walay nalista nga matang nga sama niini.[5]. ...
Superficial mycoses due to dermatophytic fungi such as represent the most common type of worldwide human being infection affecting numerous keratinized tissues in our body such as the skin hair and nails etc. of treatment. The combination of violacein and azoles prospects to the enhanced inhibition of mycelial growth and conidial germination. Vegfa Moreover combination enhanced the pace of launch of intracellular materials. Morphological changes by SEM analysis were also prominent with the combination. A normal human being cell collection [Foreskin (FS) normal fibroblast] was used to check the cytotoxicity of violacein. Violacein recorded no cytotoxicity up to 100 μg/ml Interestingly. The synergistic aftereffect of violacein and azoles against medically relevant fungi may be the most significant causative agent of fungal attacks affecting several keratinized tissue (Baltazar et al. 2013 generally affect fingernails (onychomycosis) a dermatophytosis SNX-2112 of high occurrence and its extremely ...
Introduction. Movement in and out of cells Helen Moth The cell membranes main function is to serve as a boundary between the cell and its environment. It is just like other organelles in the cell in that it serves the cell by having its own specialised jobs. In terms of beetroot the purple pigment is contained within the cell membrane, if this membrane is disrupted the pigment will be released. Temperature may be the cause of the disruption. High temperatures could distort the active site of the carrier, channel of gated proteins, therefore affecting the shape of the fluid mosaic model membrane which may release molecules that normally would not be allowed to be released (in our case the purple pigment), or allow other molecules in. Temperature basically disrupts facilitated diffusion. The picture above shows a close up view of a cell membrane, cell membranes are made up of fat molecules, or to be more specific phospholipid molecules with various large intrinsic and extrinsic, globular ...
Its a scientifically-proven fact that the darker the food, the higher the antioxidant level.. Antioxidants have the ability to clean up free radicals and keep you looking younger, longer. Thus, purple foods are known for having amazing health benefits and healing powers. scientifically-proven fact that the darker the food, the higher the antioxidant level. The purple pigment in all of these fruits and vegetables contain flavonoids, including resveratrol, which can help decrease blood pressure. Resveratrol helps relax the arterial walls, decreases the pressure in the arteries and allows better circulation. Produce with purple hues contain a variety of polyphenols that can reduce the inflammatory response in the body.. Studies have shown that anthocyanin-rich purple foods may have the potential to lower blood pressure and reduce the risk of cardiovascular disease. One USDA-funded study found that a couple of servings of purple potatoes a day helped lower blood pressure in obese and hypertensive ...
On Monday, I introduced function as a particularly useful concept in biology, but also cautioned that-like all good concepts-it has fuzzy edges. Indeed, it has lots of similarly fuzzy peers in the language of biology: for example, asking a molecular biologist What is a gene? or asking an ecologist What is a species? is not advisable unless you have an hour or more to devote to the conversation. A discussion of biological function could generate a similar conversation.. For most biologists, something biological has function if to contributes to the characteristics of an organism in such a way as to favor its reproduction (usually by favoring its survival). Conversely, for a biologist to claim that some feature of an organism is non-functional, they are claiming that this feature does not contribute to or favor survival or reproduction. To return to our historically-interesting example from yesterday, the wild-type allele of the enzyme responsible for making purple pigment in pea ...
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The development and production of cabbage (Brassica oleracea var. Royal vantage) in a greenhouse, under different osmotic pressures of the Steiner nutrient solution and biofertlized with bacterial consortiums, were evaluated. A 4 × 3 factorial design was established, where the factors were four levels of osmotic pressure (0.18, 0.36, 0.54 atm and a control without nutrient solution), and two bacterial consortia: Azospirillum brasilense + Acinetobacter calcoaceticus (AA) and Raoultella terrigena + Chromobacterium violaceum (RC), and an uninoculated control. Six repetitions were established for each treatment. Seeds inoculated with the consortium AA had the highest emergence percentage (100%), as well as the highest emergence rate (20.5), compared with the consortium RC and the non-inoculated plants. Inoculation with the consortium AA and the osmotic pressure of 0.54 atm produced taller plants (60-65%), and a stem with a larger diameter (35-46%). The weight of the fresh biomass and the ...
A case report of pneumonia and egg retention in a river terrapin was presented in this paper. A 20 year-old river terrapin (Batagur baska) was presented with complaint of coughing with blood discharge, wheezing breathing and inappetance. Plain radiograph showed both right and left lung lobes were consolidated due to pneumonia 90% and 70%, respectively. Tentative diagnosis of pneumonia due to opportunistic bacterial infection of Chromobacterium sp. and Plesiomonas shigelloides was made in this case based on bacterial isolation and response to antibiotic and anti-inflammatory drugs. Enrofloxacin and flunixin meglumine were administered from Day-1 to -5. Enrofloxacin was then changed to gentamicin since it showed resistance in those bacteria. No clinical changes were observed. Gentamicin was given at rate 2.5 mg/kg once a week intramuscularly and prednisolone 2 mg/kg once on Day-6 given intramuscularly, showed a response on Day-10 of hospitalisation with the evidence of reduction in lung ...
PMRs report on global Microbial Lipase market. The global market of Microbial Lipase is US$ xx Mn/Bn in 2018 with xx% CAGR from 2014 to 2018 and it is spectated to peg US$ xx Mn/Bn by the end of 2029 with a CAGR of xx% from 2019 to 2029. The Microbial Lipase market study analyzes the historic, current and future behavior of the Microbial Lipase market with the help of DROT analysis and Porters Five Forces analysis.. The Microbial Lipase market report has considered 2018 as the base year, 2014-2018 as the historic period and 2019-2029 as the forecast period. Important segments by product type covered in the report include product 1, product 2, product 3 and product 4. Key end uses analyzed in the research consist of end use 1, end use 2, end use 3 and end use 4.. Request Sample Report @ https://www.persistencemarketresearch.co/samples/31180. key players and products ...
Creative Enzymes (US). The report begins with the overview of the Bacteria Sourced Microbial Lipase Market and offers throughout development. It presents a comprehensive analysis of all the regional and major player segments that gives closer insights upon present market conditions and future market opportunities along with drivers, trending segments, consumer behaviour, pricing factors and market performance and estimation throughout the forecast period.. The report also covers geographical markets and key players that have adopted significant strategies for business developments. The data within the report is displayed in a statistical format to offer a better understanding upon the dynamics. The report compiles exhaustive information acquired through proven research methodologies and from dedicated sources across several industries.. To Buy Full Version Of This Report, Visit https://dataintelo.com/checkout/?reportId=127302. The report segments the Global Bacteria Sourced Microbial Lipase ...
Botanical Name : Aconitum violaceum Family : Ranunculaceae Genus : Aconitum Tibetan Common Name:: bdud rtsi lo ma, bong nga nag po (Amrita leaves, black
The Bacteria Sourced Microbial Lipase Market Report (Japan , Russia , China , France , Asia etc) 2020 Global Industry Analysis, Key Strategies, Demand, Size, Share and Regional Trends by Forecast to 2026.
[162 Pages Report] The microbial lipase market was valued at USD 400.6 Million in 2017 and is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018, in terms of value.
Externally, the crushed stems and leaves are applied as a poultice for headache, ulcerations of the nose, and on the abdomen against diarrhoea, often in association with Persicaria barbata, and the leaves of Uraria and Momordica species ...
Except where otherwise noted, this items license is described as Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License ...
Pumps and selective uptake devices need fuel - biological energy - and halobacteria, unlike Dunaliella, are not able to photosynthesise energy-yielding organic matter from carbon dioxide. They need pre-formed organic matter as food, and supplies of this are likely to be intermittent. However, their membranes do possess a unique way of obtaining energy from light. In their membranes are patches of purple pigments called rhodopsins. These are light-sensitive. Actually, they are chemically related to pigments in the retinas of our eyes, the light receptors which enable us to see. Not that halobacteria can see; in their case the pigments capture light energy and use it to generate a substance called ATP, which is the universal source of biological energy in cells…In the process of generating ATP, ions are swapped between the interior and exterior of the cell such that most of the sodium ends up outside and potassium is retained: light helps the ion pump work. This is a special kind of ...
The finale row is also one that I think will excite a lot of people. Theres a light pink that will make a great lid shade and a deep brown that will make a gorgeous brown smoky eye. Next there are three brights that probably arent for the faint of heart. The purple is stunning and manages to avoid the curse of the purple pigment and gives amazing full colour. The peacock blue is another stunner that I think Ill reach for more for the lower lashline, but gives such stunning pigment that Im tempted to play with it on the lid. Finally, theres a bright canary yellow that screams summertime. The last two shades, a burnt copper and a deep chocolate brown with a hint of burgundy, are also absolutely beautiful and will also get a lot of use by me ...
Anthocyanins are red, blue, and purple pigments distributed throughout nature. The pigments in owers are present to attract pollinators. In fruits,
ü The honors we have won: Regional Winner, Best New BioBrick Part or Device Engineered and Gold Medal at Asia Jamboree; Advanced to World Championship.. ü The breakthrough we made: Redefinition of scaffold in Synthetic Biology by recruiting E.colis inner membrane as a natural two-dimensional scaffold. ü The system we built: 6 membrane proteins orderly organized on the inner membrane of E.coli, the feasibility of which has been confirmed by fluorescence complementation assay and biosynthesis experiment.. ü Device 1 we created - Membrane Accelerator: A universal tool that serves to accelerate biochemical reactions in E.coli; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type E.coli and 9 fold compared to E.coli overexpressing cytoplasmic enzymes.. ü Device 2 we created - Membrane Rudder: A universal tool used to dynamically and artificially control biochemical reactions in E.coli; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully ...
Semi-definitive and tannin-based hair straightening that promotes strengthening and volume-reducing action. It has gel texture and a special oils blend, which nourishes and protects the strands against external aggressions. Promotes a natural straight hair, free of frizz and porosity. Developed with violet pigments, it has a toning action, which removes the undesired yellow and orange tones of blonde hair.. ...
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Bothriochloa bladhii is a perennial C4 grass widely naturalized in tropical and subtropical regions of the world. It has been intentionally...
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Diarylheptanoids from the barks of Alnus viridis ssp. viridis (green alder) and Alnus glutinosa (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Although all of the investigated compounds negatively influenced the motility of P. aeruginosa PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70%. Three of the diarylheptanoids (3, 4, and 5) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone (7) and hirsutenone (5) were able to inhibit the biosynthesis of violacein in C. violaceum CV026, with 5 being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids (1) was ...shown ...
Interpretive Summary: Pecans are economically the most important native nut crop in the USA. Among the major concerns are the pecan weevil, pecan aphids, and diseases such as pecan scab. These pests are generally controlled with broad spectrum chemicals. The chemical pesticides can be harmful to the environment and garner secondary pest outbreaks and resistance. Our overall goal for this project is to develop alternative production practices for pecans that are sustainable and effective. The research reported herein focuses on pecan weevil and aphid control and also suppression of pecan scab. The focus of the research has been on the use of novel environmentally friendly insecticides based on bacterial products. We discovered that a bacteria-based product (Grandevo®, based on a bacteria called Chromobacterium subtsugae), significantly reduced pecan weevil damage in USDA and commercial pecan orchards. Additionally, the bacterial product reduced pecan aphid populations. This is especially ...
I grew a couple different types of tomatillos (Physalis ixocarpa) this year. I collected seeds for the first one from a medium-sized, dark purple fruit I got from a local CSA. The plants didnt thrive, but they did pretty good considering the lack of care I gave them. The plants have lots of purple pigment on the stems and the fruit husks, but the fruit themselves remain bright green on the plant. Once the fruit are picked and the husk is removed, the green fruit start developing intense purple pigment in response to light. The original fruit was purple all the way through, but no fruit this year have such a pigment pattern. Im hoping the coloration of these plants indicates they are hybrids and so the darker color may turn up again in the F2 generation next year ...
similar microtubules b. Prokaryote flagella - solid structure made of long filaments of protein 5. No prokaryotes have cells II. Archaebacteria A. Bacteria appear similar under the microscope but differ biochemically B. Characteristics of archaebacteria 1. No peptidoglycan in the cell wall 2. Inhabit extreme environments C. Three groups 1. Extreme halophiles a. Occur only in extremely salty environments b. Found in salt brines used to cure fish (red patches on fish) c. Osmotic potential of cell usually within range of most bacteria d. Some are photosynthetic, using the purple pigment bacteriorhodopopsin 2. Methanogens a. Anaerobes b. Produce methane from carbon dioxide and hydrogen c. Inhabit sewage and swamps and are common in digestive tracts d. Most common of the archaebacteria 3. Thermacidophiles a. Grow in hot, acidic environments b. Common in hot sulfur springs III. Cyanobacteria (Cyanophyta) A. Occurrence 1. Ponds, lakes, swimming pools 2. Moist soils, dead logs, and the bark of trees 3. ...
What are the health benefits of red cabbage? All cabbage is very good for you, but red cabbage is definitely top of the list for me. The deep purple pigment is one of the real keys here. It is delivered by a powerful group of antioxidant compounds called anthocyanins, similar o what would be found in things such as blueberries and red wine.. Anti-inflammatory. Anthocyanins deliver reasonable anti-inflammatory activity, especially for the digestive tract and cardiovascular system. They help to reduce some of the chemicals that actually trigger inflammation in the first place. Within the cardiovascular system, flavonoids such as anthocyanins can help to protect the endothelium, the inner skin that lines the inside of blood vessels, from inflammatory damage. This is the damage that can set the stage for the whole cascade of events that lead to full blown heart disease with its calcified fatty plaques within the vessel walls.. Blood pressure. The anti-inflammatory functions outlined above are the ...
French \French\ (fr[e^]nch), prop. a. [AS. frencisc, LL. franciscus, from L. Francus a Frank: cf. OF. franceis, franchois, fran[,c]ois, F. fran[,c]ais. See {Frank}, a., and cf. {Frankish}.] Of or pertaining to France or its inhabitants. [1913 Webster] {French bean} (Bot.), the common kidney bean ({Phaseolus vulgaris}). {French berry} (Bot.), the berry of a species of buckthorn ({Rhamnus catharticus}), which affords a saffron, green or purple pigment.
The Ilam azaleas are hybrids developed in New Zealand from crosses between R. molle, R. calendulaceum, and R. viscosum. Upright, deciduous shrub that is cold
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Candidiasis is a mycosis caused by fungi of the genus Candida spp. It affects up to 50% of the worlds population and may progress to candidaemia, leading to death in up to a half of patients. Commercial drugs used in its ...

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