Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Chromatography, Gas: Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Chromatography, Liquid: Chromatographic techniques in which the mobile phase is a liquid.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Molecular Weight: The sum of the weight of all the atoms in a molecule.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Chromatography, Agarose: A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Gas Chromatography-Mass Spectrometry: A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.Chromatography, Reverse-Phase: A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Kinetics: The rate dynamics in chemical or physical systems.Chromatography, Paper: An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)SepharoseSubstrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Tandem Mass Spectrometry: A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Spectrometry, Mass, Electrospray Ionization: A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Isoelectric Focusing: Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.Countercurrent Distribution: A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)Chromatography, Micellar Electrokinetic Capillary: A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.Chemical Fractionation: Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Methods: A series of steps taken in order to conduct research.Isoelectric Point: The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Drug Stability: The chemical and physical integrity of a pharmaceutical product.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Hydroxyapatites: A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Calibration: Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Microchemistry: The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.PolysaccharidesRadioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Acetonitriles: Compounds in which a methyl group is attached to the cyano moiety.Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Ammonium Sulfate: Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.Chemical Precipitation: The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Limit of Detection: Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.Solid Phase Extraction: An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.Glycoside HydrolasesCentrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Anion Exchange Resins: High-molecular-weight insoluble polymers that contain functional cationic groups capable of undergoing exchange reactions with anions.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Brain Chemistry: Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.Chemistry Techniques, Analytical: Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Methanol: A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.Solvents: Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)Bacterial Proteins: Proteins found in any species of bacterium.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Glycopeptides: Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Flame Ionization: Pyrolysis of organic compounds at the temperature of a hydrogen-air flame to produce ionic intermediates which can be collected and the resulting ion current measured by gas chromatography.Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Ultracentrifugation: Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Spectrometry, Mass, Fast Atom Bombardment: A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Isomerism: The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Glycolipids: Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Chromatography, Supercritical Fluid: A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Biotransformation: The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Physicochemical Phenomena: The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.Detergents: Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.Chemistry, Physical: The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Cyanogen Bromide: Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.EstersTritiumDisaccharides: Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Glycosides: Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.GlucosidesBlood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.Electrophoresis, Disc: Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.Monosaccharides: Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Chymotrypsin: A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.Spectrometry, Fluorescence: Measurement of the intensity and quality of fluorescence.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Microsomes, Liver: Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Sulfates: Inorganic salts of sulfuric acid.Isotope Labeling: Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Mannose: A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Circular Dichroism: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Trifluoroacetic Acid: A very strong halogenated derivative of acetic acid. It is used in acid catalyzed reactions, especially those where an ester is cleaved in peptide synthesis.Ultrafiltration: The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Galactose: An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.Methylation: Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)Peptide Hydrolases: Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.GlucosamineTissue Distribution: Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Streptomyces: A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.Carbon Radioisotopes: Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.Electrochemistry: The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.Pesticide Residues: Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination.Affinity Labels: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.Edetic Acid: A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.Sugar Alcohols: Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)Cations, Divalent: Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.Acetates: Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Gangliosides: A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Immunosorbent Techniques: Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.Plants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.Heparin: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Electrophoresis, Paper: Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.Sequence Analysis: A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Sulfhydryl Compounds: Compounds containing the -SH radical.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Silicon Dioxide: Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.Drug Contamination: The presence of organisms, or any foreign material that makes a drug preparation impure.Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.FucoseRecombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Borohydrides: A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.Solid Phase Microextraction: A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Immunochemistry: Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Uronic Acids: Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Quality Control: A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)Glucuronates: Derivatives of GLUCURONIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the 6-carboxy glucose structure.Sodium Dodecyl Sulfate: An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.

Enrichment and characterization of histones by two-dimensional hydroxyapatite/reversed-phase liquid chromatography-mass spectrometry. (1/289)

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Application of ionic liquids in high performance reversed-phase chromatography. (2/289)

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Use of high performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the analysis of ceramide-1-phosphate levels. (3/289)

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Unambiguous determination of isobaric histone modifications by reversed-phase retention time and high-mass accuracy. (4/289)

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Primary structure and antibacterial activity of chicken bone marrow-derived beta-defensins. (5/289)

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An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. (6/289)

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In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography. (7/289)

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Intrinsic amino acid side-chain hydrophilicity/hydrophobicity coefficients determined by reversed-phase high-performance liquid chromatography of model peptides: comparison with other hydrophilicity/hydrophobicity scales. (8/289)

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*Tetrahydrofuran

THF is used as a component in mobile phases for reversed-phase liquid chromatography. It has a greater elution strength than ... it can be used to dissolve polymers prior to determining their molecular mass using gel permeation chromatography. THF ...

*Amino acid dating

"A new procedure for determining dl amino acid ratios in fossils using reverse phase liquid chromatography". Quaternary Science ... Reverse phase HPLC techniques can measure up to 9 amino acids useful in geochronology over different time scales on a single ... The amino acid derivative hydrolysis product can be combined with a chiral specific fluorescent, separated by chromatography or ... Alternatively, the particular amino acid can be separated by chromatography or electrophoresis, combined with a metal cation, ...

*Vitamin C

"Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography ( ... This verdict was reversed by the 2nd U.S. Circuit Court of Appeals in New York, on the grounds that China formally advised the ... Supreme Court announced that it would consider an appeal filed to reverse the lower court decision.[155] ... scurvy previously induced by an experimental scorbutic diet with extremely low vitamin C content could be completely reversed ...

*Vitamin C

"Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography ( ... This verdict was reversed by the 2nd U.S. Circuit Court of Appeals in New York, on the grounds that China formally advised the ... Supreme Court announced that it would consider an appeal filed to reverse the lower court decision.[155] ... scurvy previously induced by an experimental scorbutic diet with extremely low vitamin C content could be completely reversed ...

*Vitamin C

"Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography ( ... scurvy previously induced by an experimental scorbutic diet with extremely low vitamin C content could be completely reversed ...

*Cinnamomum tamala

Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography". Chromatographia. 61 (9 - 10): 443-446. doi: ...

*Eugenol

Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography". Chromatographia. 61 (9-10): 443-446. doi: ...

*Superheated water

... a clean eluent for reverse phase high performance chromatography". Analytical Communications. Royal Society of Chemistry. 33 (9 ... Reverse phased HPLC often uses methanol / water mixtures as the mobile phase. Since the polarity of water spans the same range ... The maximum temperature is limited to that at which the stationary phase is stable. C18 bonded phases which are common in HPLC ... permanent dead link] Smith, Roger; Young, E.; Sharp, B. "Superheated water chromatography - flame ionization detection" (PDF). ...

*List of Acacia species known to contain psychoactive alkaloids

"Reversed-phase high-pressure liquid chromatography of some tryptamine derivatives." Journal of Chromatography 157:365-370. ...

*Procyanidin A2

... of Proanthocyanidin A2 Content in Phenolic Polymer Isolates by Reversed-Phase High Performance Liquid Chromatography". Journal ... of Chromatography A. 1216 (9): 1403-1409. doi:10.1016/j.chroma.2008.12.086. PMID 19168185.. CS1 maint: extra punctuation (link ...

*Paroxypropione

... reverse-phase partition chromatography separation of diethylstilbestrol metabolites and analogs". Analytical Biochemistry. 92 ( ...

*Susan M. Gaines

Gaines, Susan M.; Jeffrey L. Bada (1987). "Reversed-phase high-performance liquid chromatographic separation of aspartame ... She has published peer-reviewed papers in The Journal of Organic Chemistry and the Journal of Chromatography A, as well as ... diastereomeric decomposition products". Journal of Chromatography A. Journal of Chromatography A. 389: 219-225. doi:10.1016/ ...

*F number (chemistry)

... in aqueous reversed-phase liquid chromatography. This relationship can be used to understand the significance of different ... and reversed-phase liquid chromatography". Chromatographia. 18 (1): 44-46. doi:10.1007/BF02279465. Rahman, M. Mizanur; Takafuji ... "Molecular-shape recognition of polycyclic aromatic hydrocarbons in reversed phase liquid chromatography". Chromatographia. 18 ( ... π Interactions with Noncrystalline Solid Phase for RP-HPLC". Anal. Chem. 77 (20): 6671-6681. doi:10.1021/ac050851v. List of F ...

*Modafinil sulfone

"Determination of modafinil in plasma and urine by reversed phase high-performance liquid-chromatography". Journal of ...

*Modafinil acid

"Determination of modafinil in plasma and urine by reversed phase high-performance liquid-chromatography". Journal of ...

*Impatiens balsamina

... by reversedphase high‐performance liquid chromatography". Phytochem Anal. 21 (5): 444-50. doi:10.1002/pca.1216. PMID 20931623. ...

*Hydrophobicity scales

Reversed phase liquid chromatography (RPLC) is the most important chromatographic method for measuring solute hydrophobicity. ... Non liquid phases can also be used with partitioning methods such as micellar phases and vapor phases. Two scales have been ... About a decade ago, another hydrophilicity scale was published, this scale used normal phase liquid chromatography and showed ... Vapor phases represent the simplest non polar phases, because it has no interaction with the solute. The hydration potential ...

*BIA Separations

"Reversed phase monolithic analytical columns for the determination of HA1 subunit of influenza virus haemagglutinin". Journal ... F. Švec, T. B. Tennikova, Z. Deyl Monolithic materials preparation, properties and applications Journal of Chromatography ... Journal of Chromatography A. 1218 (17): 2405-2412. doi:10.1016/j.chroma.2010.12.066. ISSN 0021-9673. Lim, Yow-Pin; Josic, Djuro ... High Performance Monolith Chromatography). During the 1999 and 2000s, the company promoted this new platform technology to the ...

*Protein sequencing

The derivatized amino acids are subjected to reversed phase chromatography, typically using a C8 or C18 silica column and an ... the amino acids are derivatized then resolved by reversed phase HPLC. An example of the ion-exchange chromatography is given by ... ion exchange chromatography cannot be used, and thin layer chromatography or high-pressure liquid chromatography should be used ... Alternatively, peptides may be desalted and separated by reversed phase HPLC and introduced into a mass spectrometer via an ESI ...

*Stercobilin

... fluorescence detection of fecal urobilins and porphyrins by reversed-phase high-performance thin-layer chromatography". Clin ...

*SNP genotyping

Denaturing high performance liquid chromatography (DHPLC) uses reversed-phase HPLC to interrogate SNPs. The key to DHPLC is the ... Oefner PJ; Underhill PA (1995). "Comparative DNA sequencing by denaturing high-performance liquid chromatography (DHPLC)". Am J ... The assay requires forward and reverse PCR primers that will amplify a region that includes the SNP polymorphic site. Allele ... solid phase which has differential affinity for single and double-stranded DNA. In DHPLC, DNA fragments are denatured by ...

*Downstream processing

Examples of operations include affinity, size exclusion, reversed phase chromatography, ion-exchange chromatography, ... Affinity chromatography often isolates and purifies in a single step. Fermentation (biochemistry) Separation process Unit ...

*Oligonucleotide synthesis

Most commonly, the crude product is desalted using ethanol precipitation, size exclusion chromatography, or reverse-phase HPLC ... 2) The second approach is only used when the intended method of purification is reverse-phase HPLC. In this case, the 5'- ... The solid phase synthesis was implemented using, as containers for the solid phase, miniature glass columns similar in their ... is purified by reverse-phase HPLC. The collected material is then detritylated under aqueous acidic conditions. On small scale ...

*Protein purification

Thirdly, proteins may be separated by polarity/hydrophobicity via high performance liquid chromatography or reversed-phase ... High performance liquid chromatography or high pressure liquid chromatography is a form of chromatography applying high ... The most common form is "reversed phase" HPLC, where the column material is hydrophobic. The proteins are eluted by a gradient ... Ion exchange chromatography is a very powerful tool for use in protein purification and is frequently used in both analytical ...

*Naja christyi

... fractions also contained toxins readily separable from the short-chain isotoxin by preparative reverse-phase chromatography. ... Reverse-phase analysis of another peak present in both venoms resolved a toxin that had an N-terminus identical to N. christyi ... Cation exchange chromatography resolved four lethal peaks from N. a. annulata venom and six lethal peaks from N. christyi venom ... The major lethal peaks (about 12% of total venom protein) were purified further with molecular sieve chromatography and were ...

*Vitamin C

"Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography ( ... This verdict was reversed by the 2nd U.S. Circuit Court of Appeals in New York, on the grounds that China formally advised the ... In June 2017 the U.S. Supreme Court announced that it would consider an appeal filed to reverse the lower court decision. The ... scurvy previously induced by an experimental scorbutic diet with extremely low vitamin C content could be completely reversed ...

*Chemistry

The phase of matter is defined by the phase transition, which is when energy put into or taken out of the system goes into ... They can be analyzed using the tools of chemical analysis, e.g. spectroscopy and chromatography. Scientists engaged in chemical ... in the case of endergonic reactions the situation is the reverse. A reaction is said to be exothermic if the reaction releases ... Another phase commonly encountered in the study of chemistry is the aqueous phase, which is the state of substances dissolved ...

*Naja annulata

... fractions also contained toxins readily separable from the short-chain isotoxin by preparative reverse-phase chromatography. ... Reverse-phase analysis of another peak present in both venoms resolved a toxin that had an N-terminus identical to N. christyi ... Cation exchange chromatography resolved four lethal peaks from N. a. annulata venom and six lethal peaks from N. christyi venom ... The major lethal peaks (about 12% of total venom protein) were purified further with molecular sieve chromatography and were ...
A simple and precise stability indicating method for the simultaneous estimation of Dapagliflozin and Saxagliptin in combined tablet dosage form was developed and validated using RP-HPLC. The chromatographic separation of the drugs was achieved with a Xterra C-18 analytical column (150 mm × 4.6 mm i.d., part
Inability of UV spectrophotometric and RP-HPLC methods to perform as stability indicating assay method for the quantification of eugenol
In the setup used in the case study (100 µL protein A column at 12 s residence time and Superdex 200 Increase 10/300), the biggest limitation lies in the 500 µl collected from the protein A column. When higher amounts were eluted from the protein A step, we lost protein as part of the peak was not collected. A solution would be to find a SEC column, onto which larger volumes can be injected, while keeping a good resolution. Increasing the residence time to, for example, 30 s will also increase the yield but triple the run time of the protein A step and consequently reduce the number of samples that can be processed. You also have to keep in mind that one should not load more than 1 mg on the Superdex 200 Increase 10/300 column.. ...
In Canada, about 68% of the population make good resolutions every year. Whether it`s going on a trip, quitting smoking or swearing, taking care of your loved
A simple, specific, accurate, and stability-indicating RP-HPLC method was developed and validated for the simultaneous determination of Trimethoprim (TMP) and Sulfadimethoxine sodium (SDMS) in Vetricine® oral solution product. The desired separation was achieved on an ODS column (250×4.6 mm i.d., 5 μm) at room temperature. The optimized mobile phase consisted of an isocratic solvent mixture of water:acetonitrile:triethylamine (700:299:1, v/v/v), adjusted to a pH of 5.7 ± 0.05 with 0.2N acetic acid. The mobile phase was fixed at 0.8 ml/min and the analytes were monitored at 254 nm using a photodiode array detector. The effects of the chromatographic conditions on the peaks USP tailing factor, column efficiency, and resolution were systematically optimized. Forced degradation experiments were carried out by exposing TMP, SDMS standards, and the oral solution formulation to thermal, photolytic, oxidative, and acid-base hydrolytic stress conditions. The degradation products were well-resolved from the
A dual retention combined with reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) has been observed on beta-cyclodextrin (beta-CD) bonded stationary phase. A typical U-shaped retention curve was achieved owing to dual retention mechanism Based on this observation, a beta-CD column can be operated under reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) modes. Two-dimensional liquid chromatography (2D-LC) analysis can be realized on Just a beta-CD column by switching these two different separation modes. In this study, off-line 2D-LC analysis for a natural product was carried out to prove the orthogonal separation between RP-LC and HILIC modes on a Click beta-CD column. Herba Hedyotis Diffusae, the whole grass of Hedyotis Diffusae wild was extracted with water, pretreated with macroporous resin and then first separated at RP-LC mode on the Click beta-CD column to obtain successive fractions, which were then ...
Pristinamycin is an antibiotic used mainly in the treatment of Staphylococcus infections. The aim of this study was to develop a rapid and simple stability-indicating RP-HPLC method for the determination of pristinamycin in tablet dosage form. Pristinamycin was eluted on the ACE-5, C18-HL, 250 x 4.6 mm, 5 µm analytical column with a mobile phase consisting of 0.2% orthophosphoric acid and acetonitrile 63:37 v/v, pumped at 1.5 ml/min flow rate. The column was maintained at 40°C and 10 μl of the solutions were injected. UV detection was performed at 206 nm. The procedure separated pristinamycin and its potential degradation products in an overall analysis time of less than 10 min with pristinamycin eluting at about 3 min. The method was validated according to the regulatory guidelines with respect to specificity, precision, accuracy, linearity, and robustness. Forced degradation studies were also performed for pristinamycin bulk drug samples to demonstrate the stability-indicating power of the HPLC
Determination of free urinary cortisol is a test of choice in the diagnosis of Cushings syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range ...
Mutalik, S., Hewavitharana, A., Shaw, P. N., Anissimov, Y. G., Roberts, M. S. and Parekh, H. S. (2009) Development and validation of a reversed-phase high-performance liquid chromatographic method for quantification of peptide dendrimers in human skin permeation experiments. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877 29: 3556-3562. doi:10.1016/j.jchromb.2009.08.039 ...
A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for simultaneous estimation of Metformin hydrochloride and Telmisartan in bulk drug and in a synthetic mixture. The analysis was carried out using Zodiac ODS C18 (4.6 x 250mm, 5μm, Make: Zodiac Life Sciences), pre-packed column. The separation was carried out using a mobile phase containing a buffer of pH 4.0, Acetonitrile and Methanol (30:30:40 v/v/v), was pumped at a flow rate of 1.4 mL/min with UV-detection at 228 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 2.523 minute for Metformin hydrochloride and 5.437 minute for Telmisartan. The method was validated and shown to be linear for Metformin hydrochloride and Telmisartan. The correlation coefficients for Metformin and Telmisartan are 0.996 and 0.998 respectively.
China Monopotassium Phosphite 98%, Find details about China Potassium Dihydrogen Phosphite, Mono Potassium Phosphite from Monopotassium Phosphite 98% - Changsha Green Mountain Chemical Co., Ltd.
A simple and reliable reversed-phase high-performance liquid chromatographic (HPLC) method for the routine determination of vitamins A and E and β-carotene in plasma (or serum) with wavelength-programmed ultraviolet-visible absorbance detection is described. A 200-μl aliquot of serum or plasma sample, after deproteinization with ethanol, and containing tocopherol acetate as internal standard, was extracted with butanol-ethyl acetate. Sodium sulphate was added for dehydration. Analytes of extracted samples were found to be stable for at least four days. A 10-μl aliquot of this organic extract was used for HPLC analysis. The mobile phase was methanol-butanol-water (89.5:5:5.5, v/v) and the flow-rate was set at 1.5 ml/min. The analytes of interest were well separated from other plasma constituents within 22 min at 45°C. The lowest detection limits of vitamins A and E and β-carotene were 0.02, 0.5 and 0.1 μg/ml, respectively. The recovery and reproducibility of the present method were around ...
A simple reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine and Olmesartan in bulk and tablet form. Chromatography was performed by isocratic reverse phase separation on a stainless steel column 4.6 x 150mm, symmetry column packed with octa decyl silane bonded to porous silica (C18) with particle size 5 micron with mobile phase containing TEA Buffer of pH 3.0 and Acetonitrile in proportion of 25:75 respectively. The flow rate was 1.0 ml/ min and effluent was monitored at 258 nm. The retention times were 2.39 min and 3.33 min respectively. The standard curve was linear over a working range of 05-35 μg/ml for both Amlodipine and Olmesartan and gave an average correlation coefficient of 0.999, and 0.999 for Amlodipine and Olmesartan respectively. The limit of quantitation (LOQ) of this method was 2μg/ml for Amlodipine and Olmesartan. The absolute recovery was 100% for Amlodipine and 100.3 for Olmesartan. Degradation products produced as a result of stress ...
Zirconia is known to be one of the best materials for the chromatographic support due to its excellent chemical, thermal and mechanical stability. In this work we report preparation and use of bovine...
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Objective: To develop a validated stability-indicating analytical method for simultaneous estimation of Atenolol (ATN) and Hydrochlorothiazide (HTZ) in pharmaceutical combined dosage form by HPTLC. Method: A high performance thin layer chromatographic (HPTLC) method has been developed for the separation of ATN and HTZ on plates precoated with aluminium back silica gel 60 F254. Different mobile phases were used on trial and error basis for separation of two drugs. The final mobile phase selected for analysis was n-butanol: ethyl acetate: methanol: tetrahydrofuran in the ratio (1:2:2:1 v/v). Both the drugs showed maximum absorbance at 250 nm which was selected as the detection wavelength throughout the experimental work. Developed method was validated as per ICH guidelines. Forced degradation of drugs was carried out under various stress conditions and HPTLC method was used for analysing the stability of drugs. Result: HPTLC method was successfully developed for separation of ATN and HTZ with good ...
Fortschr Med. 1987 Aug 30;105(25):63. German.. Determination of (E)-1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-(1-methyl-2- phenylethenyl) naphthalene, an antiacne agent, and its phenolic metabolite in plasma by reversed-phase high-performance liquid chromatography ...
HPLC Application #18828: Atenolol and Related Substances CRS using Kinetex 2.6um C18 100 x 4.6mm at 01.3ml/min. Column used: Kinetex® 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea Part#: 00D-4462-E0
The test the FDA uses to certify each batch of these dyes before they legally may be used in drugs and cosmetics marketed in the United States no longer will be done by visible spectrophotometry, but by reversed-phase high performance liquid chromatography ...
DEVELOPMENT AND VALIDATION OF NOVEL SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF PIOGLITAZONE AND METFORMIN IN BULK AND FIXED DOSAGE FORMS BY AREA UNDER CURVE AND DUAL WAVELENGTH MODE
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Disclosed is a single phase liquid composition comprising 0.5 to 30 wt % of a benzotrifluoride solvent, about 0.5 to about 50 wt % of a glycol ether cosolvent, up to about 50 wt % of an alkanol, up to about 10 wt % of a surfactant, and the remainder water. The composition is effective in cleaning hard surfaces, stripping paint, and as a carrier.
This paper outlines the development of a liquid chromatography method for simultaneous determination of approximately 20 amino acids in simple and complex mixtures that complies with the system suitability requirements of the Ph. Eur. general chapter (2.2.56).
Selenium (Se) and sulfur (S) speciation analysis in edible and medicinal Se enriched P. pulmonarius extracts was performed. Mycelium, colonized substrate, and fruiting bodies at different harvesting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS detector. Extraction efficiencies in enzymatically digested and aqueous extracts were between 45.3 and 109% fo ...
|p|Silia|em||span style=color: #f37021;|Bond|/span||/em||sup|®|/sup| C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercially available. Considered as the l
|p |Silia|em||span style=color: #f37021;|Bond|/span||/em||sup|®|/sup| Phenyl (Si-PHE) is a sorbent of medium polarity used in reversed-phase chromatography. It has similar retention than C8 but with different selectivity especially for molecules con
This pill with imprint APO ESC 20 is White, Round and has been identified as Escitalopram oxalate 20 mg (base). It is supplied by Apotex Corp..
Dear Everyone: I am trying to transfer for western blot after Tris-Tricine-buffered electrophoresis which looks good for low molecular protein. But, it looks very difficult to transfer onto PVDF membrane with 1200mA.hr. There is no critical difference except containing glycerol(I think is for good resolution). Anyway, although I tried it under the condition, parts of high molecular bands are remained on gel. What should I do for complete transfer? I would appreciated if anyone who know trouble shooting make me know. Thank you. Sincerely yours, SK Park ...
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A stability-indicating liquid chromatographic method was developed and validated for simultaneous determination of quinapril and hydrochlorothiazide in drug substances and dosage forms. Chromatographic separation of quinapril, hydrochlorothiazide and its degradation products was achieved on a RP-18 column, using acetonitrile and phosphate buffer (pH 4.6) as mobile phase in a gradient mode and detection at 216 nm. Stress testing was performed under hydrolytic, oxidative, thermal and photolytic conditions. The degradation products were well resolved from main peaks, proving the stability-indicating power of the method. The assay was linear for quinapril and hydrochlorothiazide concentrations of 40-200 µg mL−1 and 25-125 µg mL−1, respectively. The developed method was selective, accurate and precise for quinapril and hydrochlorothiazide determination. This method was used to quantify both drugs in combined commercial tablets. The results showed that the proposed method was found to be ...
Elaeagnus angustifoliaLinn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to classify and...
A coupled-column liquid chromatographic method for the direct analysis of 14 urinary nucleosides is described. Efficient on-line clean-up and concentration of 14 nucleosides from urine samples were obtained by using a boronic acid-substituted silica column (40 turn x 4.0 mm I.D.) as the first column (Col-1) and a Hypersil ODS2 column (250 mm x 4.6 mm I.D.) as the second column (Col-2). The mobile phases applied consisted of 0.25 mol/L ammonium acetate (pH 8.5) on Col-1, and of 25 mmol/L potassium dihydrogen phosphate (pH 4.5) on Col-2, respectively. Determination of urinary nucleosides was performed on Col-2 column by using a linear gradient elution comprising 25 mmol/L potassium dihydrogen phosphate (pH 4.5) and methanol-water (60:40, v/v) with UV detection at 260 nm. Urinary nucleosides analysis can be carried out by this procedure in 50 min requiring only pH adjustment and the protein precipitation by centrifugation of urine samples. Calibration plots of 14 standard nucleosides showed ...
B162 α-Tocopherol supplementation has been shown to prevent prostate cancer, yet studies of dietary and serologic vitamin E have been mixed for this malignancy. We conducted a nested case-control study of serum tocopherols and prostate cancer within the cancer screening arm of the PLCO trial among men ages 55-74 years old. Blood samples were obtained at baseline, shipped overnight to a biorepository, and stored at -70 oC. Among cases of prostate adenocarcinoma identified through October 1, 2001, 803 were diagnosed at least one year after blood draw among non-Hispanic white men. Incidence-density sampling was used with frequency-matching by age, time since initial screening, and year of blood draw to select controls (1.2 ratio to cases). Serum concentrations of α- and γ-tocopherol were determined for cases (n=680), controls (n=824), and batch-based quality control (n=171) samples using reversed-phase high-performance liquid chromatography with UV detection. Coefficients of variation were 5.8% ...
PMID 3401329] Determination of neutral haemoglobin variants by immobilized pH gradient, reversed-phase high-performance liquid chromatography and fast-atom bombardment mass spectrometry: the case of a Hb Torino alpha 43 (CE1) Phe----Val. ...
TY - JOUR. T1 - Development and validation of analytical method for the estimation of omeprazole, domperidone and their related substances. AU - Srinivas, P.. AU - Siva Rao, T.. AU - Ramakrishnan, E.. AU - Gowda, D.V.. N1 - cited By 2. PY - 2013. Y1 - 2013. M3 - Article. VL - 4. SP - P906-P911. JO - International Journal of Pharma and Bio Sciences. JF - International Journal of Pharma and Bio Sciences. SN - 0975-6299. IS - 2. ER - ...
Micellar liquid chromatography (MLC) is an analytical technique belonging to the wide range of reversed-phase liquid chromatographic (RP-LC) separation techniques. MLC with the use of surfactant solutions above its critical micellar concentration (CMC) and the addition of organic modifiers is currently an important analytical tool with still growing theoretical considerations and practical applications in pharmaceutical analysis of drugs and other biologically active compounds. The use of MLC as an alternative, relatively much faster in comparison to conventional chromatographic separation techniques has several advantages, especially as being suitable for screening pharmaceutical analysis. The analytical data received from MLC analysis are considered a useful source of information to predict passive drug absorption, drug transport and other pharmacokinetics and physicochemical measures of pharmaceutical substances. In the review several MLC assays for determination of drugs and other active ...
Each ml of this solution contains 1 mmol of potassium ions, 1 mmol of phosphate ions, and 2 mmol of hydrogen ions. Lets dwell on that for a moment. The compound is composed of K+, 2H+ and PO43-. In fact it very closely resembles phosphoric acid (H3PO4) except one of the hydrogen atoms has been substituted with potassium.
Gradient elution of polystyrene standards on reversed phase C18 columns by methanol/tetrahydrofuran or methanol/dichloromethane mixtures yielded a strange effect of the molecular weight of the sample...
The requirement of CBD strains comes from the vast medical benefits of CBD (cannabidiol) and the non-psychoactive nature of the ...
After the successful inaugural conference in IICT, Hyderabad last year, this conference has been conceptualized to discuss the advanced techniques and applications of Mass Spectrometry. The experts in this conference will cover various advanced techniques followed by novel applications of Mass Spectrometry in analysis of Pharmaceutical and Forced Degradation products, Metabolites, Food, Environment, Forensic, Clinical and Biological samples. This event will provide you with excellent opportunity to enhance your knowledge on Mass Spectrometry as well as learn the advanced applications in different fields. Further, this event will also help in networking with like minded peers, helping you to build new relationships and optimize your workflow ...
Vadim- I have re-nickelated Ni-NTA-agarose resins with varying success. I believe Qiagen provides a protocol for doing this. If I remember correctly, it involves stripping the resin with EDTA and then passing nickel sulfate over the column. Alternatively, I have also used Ni-NTA columns many more than 5 times and have had pretty good resolution. Since these resins are available for about $5/ml, I would suggest using the resin as long as you still get good resolution and then either replace it (if it is a relatively small volume), or attempting to regenerate it if it is a large volume ...
Prepare the following solutions in a mixture of 92 volumes of mobile phase A and 8 volumes of mobile phase B (dissolution solvent).. For solution (1) dissolve 5.0 mg of hypoxanthine R in the dissolution solvent and dilute to 100.0 ml with the same solvent. Dilute 1.0 ml to 20.0 ml with the same solvent. For solution (2) dissolve 5 mg of didanosine for system suitability RS (containing impurities A to F) in the dissolution solvent and dilute to 10 ml with the same solvent. For solution (3) dissolve 25 mg of the test substance in the dissolution solvent and dilute to 50.0 ml with the same solvent. For solution (4) dilute 5.0 ml of solution (3) to 50.0 ml with the dissolution solvent. Then dilute 5.0 ml of this solution to 50.0 ml with the same solvent.. Operate with a flow rate of 1.0 ml per minute. As a detector use an ultraviolet spectrophotometer set at a wavelength of about 254 nm.. Use the chromatogram supplied with didanosine for system suitability RS and the chromatogram obtained with ...
Validation of Compendial Procedures (3). In addition, USPC proposed appropriate system suitability criteria and specifications and developed two new reference standards (RS) to support the revised monographs: USP heparin sodium identification reference standard (RS) and USP heparin sodium system suitability RS.. The revised monographs were posted June 18, 2008, on the USPC website ) as Revision Bulletins and became official immediately. These monographs are referenced on the FDA website as well and have played an important role in controlling the quality of heparin API in the US (1).. Before posting the heparin Revision Bulletins, USPC solicited feedback from stakeholders by hosting two online meetings in spring 2008. Industry comments as well as submissions of new and improved analytical methods for characterization of heparin were critical in shaping the next stage of the monograph revision.. Stage 2: Comprehensive modernizationUSPC and involved stakeholders realized that a thorough ...
... : Chromatograms of the analyte standard solutions with the mobile phase composed of 50 mM potassium dihydrogen phosphate (pH 3.05) with 0.01% diethylamide and different proportion of methanol ...
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Majji AB, Humayun MS, Viagra silagra JD, Suzuki S, Dвanna SA, de Juan E, Jr (1999) OOvernight histological and electrophysiological results of an inactive prescrription electrode array implantation in dogs. 25 gl solution of potassium dihydrogen phosphate R (1090 VV).
[Determination of taurine in foods by high performance liquid chromatography].: Taurine (2-aminoethanesulfonic acid) was quantitated by reversed-phase liquid ch
Title: MIL-STD-1844, Version: -, Date: 1984-Mar-30, Desc: GAS CHROMATOGRAPHY METHOD FOR DETERMINATION OF TRACE CHLORINATED SOLVENTS HYDRAULIC FLUID (30 MAR 1984
View Notes - IMG_0001_NEW_0037 from CHEM 282 at Simon Fraser. If it has a greater affinity for the mobile phase it will move along the plate faster with the mobile phase and if it has a greater
Could anyone help me on this?I am looking at a 106kDa protein in retina. electrophoresis is excellent but only for smaller proteins. I dont get good resolution of high molecular weight proteins, actually I might not even get bands above 70kDa. I have tried different gel concentrations (7-12%)(Tris gels). at 7% the whole sample doesnt run well as if not good homogenisation isdone. at higher percentages I get the previously described motif, so what happens to my high MW ptroteins? do the become fractionated and fall as lower MW?but then the Abd should detect the fraction that has its detection sequence at a lower MW band. do they stay in the membrane during homogenisation and so are located in the precipitant of the centrifugion which goes to the rubbish ...
A process for forming on any printing stock, single colored and multi-colored images using the expose-in-register and laminate-in-register processes is described. Images having high quality, good resolution, and color flexibility not heretofore economically feasible are obtained using the process of the invention.
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An immiscible lipophilic or hydrophilic liquid phase is separated respectively from a continuous hydrophilic or a lipophilic phase liquid. Fibers having hydrophilic and hydrophobic properties are form
Abstract:. Introduction: In this paper a fast and novel stability-indicating ultra fast LC method for separation and estimation of impurities in clopidogrel and aspirin in their combined tablet dosage form and omeprazole was developed and validated according to ICH guidelines. Methodology: The separation of USP related substances of clopidogrel (A, B and C), aspirin (D), omeprazole (A, B and C) and few other unknown impurities was detected by using ultra fast liquid chromatography with PDA detection. The maximum detection was set as follows: 237 nm for aspirin, its impurities and for the impurity C of clopidogrel and 254 nm for Clopidogrel and its impurities except for impurity C and 280 nm for omeprazole and its impurities. Phenomenex C8 (250 mm × 4.6 mm, 5 μ) was used as a stationary column to separate and analyze the mixture within 11 min with a programmed gradient elution of 0.01 M phosphate buffer pH 2.0 and acetonitrile. The tablets were exposed to acid, alkaline, thermal, higher ...
A comparative study on the tapering of natural quartz and potassium dihydrogen phosphate (KDP) crystals has been made. Both tapered natural quartz crystals and KDP crystals display similar surface microtopography featuring a large number of tiny elongated growth islands and striations on the tapered regions. In addition to the well accepted fact that trivalent metallic ions in aqueous solution can cause the tapering of KDP crystals, we find that Pb2+ has the same effect. This possibly originates from preferentially settled impurities and embryonic crystallites along the edges between two major faces. © 1995 ...
DEVELOPMENT AND VALIDATION OF A RP-HPLC FOR THE SIMULTANEOUS ESTIMATION OF ATENOLOL AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORMS
b) THE ABOVE-MENTIONED WARRANTIES IN CLAUSE 8(A) ABOVE ARE GIVEN AND ACCEPTED IN SUBSTITUTION FOR, AND VWR HEREBY DISCLAIMS ALL OTHER WARRANTIES OR GUARANTEES WITH RESPECT TO THE SUBJECT MATTER OF THIS AGREEMENT, WHETHER STATUTORY, WRITTEN, ORAL, EXPRESS OR IMPLIED INCLUDING, WITHOUT LIMITATION, ANY WARRANTY OF MERCHANTABILITY, SUITABILITY OR FITNESS FOR A PARTICULAR PURPOSE. YOU ACKNOWLEDGE THAT YOU DO NOT RELY ON AND WAIVE ANY CLAIM FOR BREACH RELATING TO ANY ADVICE, REPRESENTATION OR WARRANTY THAT IS NOT EXPRESSLY SET OUT HEREIN. IN PARTICULAR, WITHOUT LIMITING THE ABOVE, YOU ACKNOWLEDGE THAT THE PRODUCTS ARE NOT CUSTOMIZED FOR YOUR SPECIFIC USE OR ORDER AND VWR CANNOT AND DOES NOT MAKE ANY REPRESENTATION OR WARRANTY THAT THE PRODUCTS ARE FIT FOR ANY PARTICULAR PURPOSE, EVEN WHERE SUCH PURPOSE WAS MADE KNOWN TO VWR, AND YOU ACKNOWLEDGE THAT YOU DO NOT RELY ON ANY SUCH REPRESENTATION OR WARRANTY.. (c) The liability of VWR under this limited warranty does not extend to any Products which are ...
Expertise:. » Isolation, characterization and evaluation of bioactive constituents from herbal extracts using bioactivity guided fractionation. » Computer aided Drug Design (in silico analysis), synthesis and pharmacological evaluation (in vitro analysis) » Resolution of drugs, including enzymatic resolution and its monitoring. » Chiral chromatography » Forced degradation studies, Impurity profiling and development of stability indicating assays. » Analytical method development and validation » Synthesis, purification and characterization of impurities/metabolites. » Structural Elucidation using spectroscopic techniques » Bioanalytical method development and validation; Therapeutic Drug Monitoring » Drug metabolism and pharmacokinetic analysis » Herbal Analysis » In vitro analysis for antibacterial, antifungal and antiamoebic activity Go to TOP ...
Pregnancy Category C. In a rat embryo/fetal development study, oral administration of escitalopram (56 mg/kg/day, 112 mg/kg/day, or 150 mg/kg/day) to pregnant animals during the period of organogenesis resulted in decreased fetal body weight and associated delays in ossification at the two higher doses (approximately ≥ 56 times the maximum recommended human dose [MRHD] of 20 mg/day on a body surface area [mg/m2] basis). Maternal toxicity (clinical signs and decreased body weight gain and food consumption), mild at 56 mg/kg/day, was present at all dose levels. The developmental no-effect dose of 56 mg/kg/day is approximately 28 times the MRHD on a mg/m2 basis. No teratogenicity was observed at any of the doses tested (as high as 75 times the MRHD on a mg/m2 basis).. When female rats were treated with escitalopram (6 mg/kg/day, 12 mg/kg/day, 24 mg/kg/day, or 48 mg/kg/day) during pregnancy and through weaning, slightly increased offspring mortality and growth retardation were noted at 48 ...
Chromatography Research International is a peer-reviewed, Open Access journal that publishes original research and review articles dealing with the use of chromatographic techniques in all areas of biological and physical science.
Non-Aqueous Phase Liquids (NAPLs) include commonly occurring organic contaminants such as gasoline, diesel fuel and chlorinated solvents. When released to subsurface environments their spreading is a complex process of multi-component, multi-phase flow. This work has strived to develop new models and methods to describe the spreading of NAPLs in heterogeneous geological media.. For two-phase systems, infiltration and immobilisation of NAPL in stochastically heterogeneous, water-saturated media were investigated. First, a methodology to continuously measure NAPL saturations in space and time in a two-dimensional experiment setup, using multiple-energy x-ray-attenuation techniques, was developed. Second, a set of experiments on NAPL infiltration in carefully designed structures of well-known stochastic heterogeneity were conducted. Three detailed data-sets were generated and the importance of heterogeneity for both flow and the immobilised NAPL architecture was demonstrated. Third, the laboratory ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Deuterated L-arginine phosphate is a promising non-linear optical material having good non-linear coefficient, high damage threshold as compared to L-arginine phosphate(LAP) and potassium dihydrogen phosphate (KDP).
V. Lacivita, M. R rat, B. Kirtman, M. Ferrero, R. Orlando, R. Dovesi Calculation of the dielectric constant ε and first nonlinear susceptibility χ(2) of crystalline potassium dihydrogen phosphate by the coupled perturbed Hartree-Fock and coupled perturbed Kohn-Sham schemes as implemented in the CRYSTAL code J. Chem. Phys.,131, 204509 (2009 ...
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Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) are unique biomarkers of alcohol use. EtG and EtS analysis offers many advantages for abstinence monitoring including the detection window, stability in stored specimens, and specificity. EtG and EtS are both polar, making them difficult to retain via reversed-phase chromatography. Both compounds are also very sensitive to matrix interferences which can result in being unable to achieve low limits of detection and can also make quantitation impossible. In this study, a simple dilute and shoot method was developed and validated for the analysis of EtG and EtS in human urine by LC-MS/MS ...
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Graduates of Brigham Young University - the names, photos, skill, job, location. Information on the Brigham Young University - contacts, students, faculty, finances.
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Question - Taking Stalopham for anxiety. How to quit this tablet?. Ask a Doctor about uses, dosages and side-effects of Escitalopram oxalate, Ask a Psychiatrist
Detailed dosage guidelines and administration information for Ibuprofen PM (diphenhydramine citrate and ibuprofen). Includes dose adjustments, warnings and precautions.
Yeah, yeah I know, they never work but this is different. This year my friend Maggie talked me into a different kind of resolution. Each month we pick a resolution and work on it. January is NO t.v. month. The idea is to make better use of your time. I know you are thinking what could be more important than Law and Order, I dont know either, Maggie can be very persuasive, if you know her be careful not to look into her eyes when she is coming up with some new scheme. February is be more social month. for Maggie that means phone calls letters and emails for me that means having people over to my house. More frightening than you know as i am about 3 magazines shy of a guest stint on hoarders. We are also doing a month of clearing out clutter and a month of being more creative. Email me back if you would like to join the challenge. I know it seems very cliche but cliches come about because often they are true. Maggie has a whole list of good resolutions but true to form i only memorized February ...
The AIDS Law Project (ALP) and the Treatment Action Campaign (TAC) welcome the opportunity to make written submissions on the draft pricing regulations. Again we express our support for the implementation of a transparent pricing system, which we believe has the potential significantly to increase access to affordable medicines for all people in South Africa.. While we recognise and support the principled basis underpinning the draft regulations, we are nevertheless of the view that in a number of key respects, the draft regulations do not give full and proper effect to the National Drug Policy of 1996, the Medicines and Related Substances Control Amendment Act, 90 of 1997, and most important, the constitutional right of access to health care services. If implemented in their current form, we believe the draft regulations will fall short of discharging the stateÕs positive constitutional obligations in respect of promoting the right of access to essential medicines. It is in respect of such ...
For example, people use terms like ввdirectionality,вв ввblobbiness,вв ввrandomness,вв and so on. System suitability в resolution minimum of 5.
System suitability reference solution (c) в resolutionminimum5betweenthepeaksduetolactitol and glycerol. The use of intraoperative TEE represents another major advance in cardiac anesthesia.
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF ZALTOPROFEN AND PARACETAMOL IN COMBINED DOSAGE FORM ...
​For the 40th time, thousands of women have come to campus to be uplifted and inspired at BYU Womens Conference, which is co-sponsored by the Relief Society. Here are highlights from a handful of those addresses.
Economizer water-wall damages initiated by feedwater impurities Sonja M. Vidojković 1, Antonije E. Onjia 2, Aleksandar B. Devečerski 3, Nebojsa N. Grahovac 3, Aleksandra B. Nastasović 1 1 University of
A new high-performance liquid chromatographic method was developed for quantification of midazolam in plasma samples from intensive care unit patients on long-term intravenous infusion of this benzodiazepine. Plasma samples (0.5 ml) were mixed with 1 microgram flurazepam (internal standard), alkalinized with 2.5 N NaOH, and extracted with toluene. The organic phase was evaporated to dryness, and the residue was dissolved in the mobile phase (acetonitrile/0.05 M phosphate buffer pH 4.5) and injected into the analytical column (C18 Nova-Pak 3.9 x 150 mm, 4 microns, maintained at room temperature; mobile phase flow rate: 1.2 ml/minute). The eluate was monitored at 207 nm, which reduced the risk of interferences from concurrent medications. Retention times of flurazepam, 1-hydroxymidazolam (an active metabolite) and midazolam were approximately 4.5, 6.1 and 13.5 minutes, respectively. The assay was linear over the range 100 to 3000 ng/ml. The coefficients of variation of the within-day and ...
Purpose: To evaluate the influence of α-crystallin racemization on lens membrane fluidity using the mild heating model based on lens epithelial cells (LECs).. Methods: LECs (SRA01/04) heated at 500C were used as the mild heating model to simulate the ageing process in vitro. After incubation under 500C for 0min, 15min, 30min, 45min and 60min, α-crystallins in LECs were isolated using gel filtration chromatography and processed with acidolysis. The purified samples were treated with o-phtalaldehyde and N-tertbutyloxycarbonyl-L-cysteine for pre-column derivatization before racemization analysis using reversed-phase high performance liquid chromatography (RP-HPLC). To detect the α-crystallin racemization, we calculated the D/L (D/L amino-acid residual) ratio according to the peak area in each chromatogram. Laurdan staining and two-photon confocal microscopy were applied to analyze the lens membrane fluidity of each group with different heating time. Immunofluorescence staining were used to ...
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METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF TOLPERISONE HCl IN BULK AND IN TABLET DOSAGE FORM AND ITS STRESS DEGRADATION STUDY USING SPECTROPHOTOMETRIC METHOD ...
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Abstract: A simple precise, accurate RP-HPLC method has been developed and validated for bioavailability study of pirfenidone in wister rat plasma. The separation and quantization of pirfenidone was achieved on a C18 reversed phase column using the mobile phase in gradient mode constituting of eluant A HPLC grade water (adjusted to pH 3.5) and eluant B 20% acetonitrile and 15% of methanol in the ratio of 60: 40 at a flow rate 1 mlmin-1. Eluted components were detected at 324 nm. The method showed good linearity for Pirfenidone in the range of 50-250ng mL-1, Y=54.97x - 349.5and correlation coefficient R2 is 0.998 respectively. The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 12 and 20 ng mL-1respectively.Thedeveloped method shows good accuracy and precision. . Accuracy ranges from 98.49% to 99.37% with the precision 6.43% to 7.67% in inter-day method. Intra-day method the accuracy ranges from 98.64% to 99.33%with the precision 5.64% to 6.93 %. For bioanalytical study, ...
Brigham and Womens / Mass General Health Care Center- Better Health in 2017. Brigham and Womens/Mass General Health Care Center at Patriot Place wants to help you make your 2017 happy and healthy.. 1. Fight the Flu. The best way to prevent getting the flu is to receive a flu shot - recommended by the Centers for Disease Control and Prevention for all patients six months and older. See your primary care physician or go to a local pharmacy to get your shot today.. If you are feeling flu-like symptoms, see your primary care physician or visit your nearest Urgent Care Center. Urgent Care Center at Brigham and Womens/Mass General Health Care Center. Learn more about the flu:. Quiz: Whats True About the Flu Dont Let the Flu Sneak Up on You. 2. Stay on Top of Your Annual Health Screenings. Early diagnosis and treatment of colorectal, breast, prostate and skin cancers are important in fighting the battle against these diseases. Learn more about when you should be screened and steps you can take to ...
Background: Ginger, the rhizome of Zingiber officinale Roscoe (family Zingeberace) has been used as a spice throughout the world since times immemorial. Ginger has been valued as a medicinal herb in several countries and has been reported to possess carminative, anti-emetic, anti-nauseate and anti-inflammatory properties. Aim: Chromolith reverse phase liquid chromatographic method for simultaneous determination, quantification and validation was developed for 6, 8, 10-gingerols and shogaol in the dry rhizome of Zingiber officinale. Materials and Methods: he method was in accordance to the International Conference on Harmonisation. It is highly specific, exhibited good linearitys (r² > 0.9998) with high precision and achieved good accuracies between 97.81 and 100.84% of quantitative results. For the developed chromolith LC method, system suitability parameters like K prime, selectivity, resolution, USP resolution, asymmetry, USP tailing, symmetry factor, USP plate count and peak purity were ...
Description of disease Fatty aldehyde dehydrogenase deficiency. Treatment Fatty aldehyde dehydrogenase deficiency. Symptoms and causes Fatty aldehyde dehydrogenase deficiency Prophylaxis Fatty aldehyde dehydrogenase deficiency
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Comprehensive and off-line supercritical fluid chromatography: reversed phase liquid chromatography for the analysis of complex...Comprehensive and off-line supercritical fluid chromatography: reversed phase liquid chromatography for the analysis of complex...

Comprehensive and off-line supercritical fluid chromatography: reversed phase liquid chromatography for the analysis of complex ... "Comprehensive and Off-line Supercritical Fluid Chromatography: Reversed Phase Liquid Chromatography for the Analysis of Complex ... "Comprehensive and Off-line Supercritical Fluid Chromatography: Reversed Phase Liquid Chromatography for the Analysis of Complex ... reversed phase liquid chromatography for the analysis of complex triglyceride profiles. High Performance Liquid Phase ...
more infohttps://biblio.ugent.be/publication/816990

Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites,...Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites,...

A procedure based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) is described for the determination of nicotine ... Chromatography was performed on a C(8) reversed-phase column using a gradient of 50 mM ammonium formate, pH 5.0, and ... Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites, ... Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites, ...
more infohttps://pubmed.ncbi.nlm.nih.gov/17640086/

Reversed-phase ion-interaction chromatography of metal ions by EDTA precomplexation | SpringerLinkReversed-phase ion-interaction chromatography of metal ions by EDTA precomplexation | SpringerLink

Conditions for the isocratic separation by reversed-phase ion-interaction chromatography of Bi(III), Fe(III), Co(III), Ni(II), ... Conditions for the isocratic separation by reversed-phase ion-interaction chromatography of Bi(III), Fe(III), Co(III), Ni(II), ... Column liquid chromatography Ion-interaction chromatography EDTA complexes Heavy metal ions This is a preview of subscription ...
more infohttps://link.springer.com/article/10.1007/BF02279472

IJMS | Free Full-Text | Reverse Phase Thin Layer Chromatography of Aminoalkanethiosulfuric Acids, Mercaptoalkanamines and...IJMS | Free Full-Text | Reverse Phase Thin Layer Chromatography of Aminoalkanethiosulfuric Acids, Mercaptoalkanamines and...

... mercaptoalkanamines and aminoalkyl disulfides were determined by reverse phase thin layer chromatography. The Rm values were ... Reverse Phase Thin Layer Chromatography of Aminoalkanethiosulfuric Acids, Mercaptoalkanamines and Aminoalkyl Disulfides. Maria ... "Reverse Phase Thin Layer Chromatography of Aminoalkanethiosulfuric Acids, Mercaptoalkanamines and Aminoalkyl Disulfides." Int. ... Reverse Phase Thin Layer Chromatography of Aminoalkanethiosulfuric Acids, Mercaptoalkanamines and Aminoalkyl Disulfides. ...
more infohttp://www.mdpi.com/1422-0067/3/7/755

Unusual signal enhancement in reversed-phase chromatography of polystyrene monitored by UV detector | SpringerLinkUnusual signal enhancement in reversed-phase chromatography of polystyrene monitored by UV detector | SpringerLink

Gradient elution of polystyrene standards on reversed phase C18 columns by methanol/tetrahydrofuran or methanol/dichloromethane ... Unusual signal enhancement in reversed-phase chromatography of polystyrene monitored by UV detector. ... Gradient elution of polystyrene standards on reversed phase C18 columns by methanol/tetrahydrofuran or methanol/dichloromethane ... Column liquid chromatography UV detection Solubility Polystyrene Sample precipitation Gradient elution This is a preview of ...
more infohttps://link.springer.com/article/10.1007/BF02259416

Reversed-phase chromatography - WikipediaReversed-phase chromatography - Wikipedia

Reversed-phase chromatography (also called RPC, reverse-phase chromatography, or hydrophobic chromatography) includes any ... hence the term reversed-phase chromatography. Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result ... "reversed-phase chromatography". Akul Mehta (December 27, 2012). "Principle of Reversed-Phase Chromatography HPLC/UPLC (with ... This technique is known as reversed-phase ion-pairing chromatography. Aqueous normal-phase chromatography IUPAC, Compendium of ...
more infohttps://en.wikipedia.org/wiki/Reversed-phase_chromatography

Detecting Barbiturates by Reversed Phase Liquid Chromatography | SelectScienceDetecting Barbiturates by Reversed Phase Liquid Chromatography | SelectScience

This application note presents a method of extracting barbiturates from urine and plasma samples by using Tox-Elut solid phase ... Application Note: Detecting Barbiturates by Reversed Phase Liquid Chromatography. 5 May 2010 ... This application note presents a method of extracting barbiturates from urine and plasma samples by using Tox-Elut solid phase ... Latest: Three Novel Ways Scientists are Using Chromatography to Keep Environmental Contaminants... ...
more infohttp://www.selectscience.net/application-notes/cecil-instruments-limited/detecting-barbiturates-by-reversed-phase-liquid-chromatography/?artID=20052

Validated quantitation of underivatized amino acids in human blood samples by volatile ion-pair reversed-phase liquid...Validated quantitation of underivatized amino acids in human blood samples by volatile ion-pair reversed-phase liquid...

... quantitation of underivatized amino acids in human blood samples by volatile ion-pair reversed-phase liquid chromatography ... A validated method that is based on the use of volatile ion-pair liquid chromatography coupled to stable isotope dilution ... as mobile phase modifiers. The collisionally activated dissociation spectra of the amino acids were investigated and the ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/12033304?dopt=Abstract

Normal and reversed phase thin layer chromatography data in quantitative structure-activity relationship study of compounds...Normal and reversed phase thin layer chromatography data in quantitative structure-activity relationship study of compounds...

Normal and reversed phase thin layer chromatography data in quantitative structure-activity relationship study of compounds ... Thin layer chromatography was performed on silica gel NP 60F(254) and silica gel RP2 60F(254) (silanized) plates impregnated ... with two mobile phases - the systems were chosen as models of drug-5-HT-receptor interaction. Relationships between ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/21570927

IJMS | Free Full-Text | Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid...IJMS | Free Full-Text | Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid...

... have successfully been developed for naturally occurring phenolic compounds in a reversed-phase liquid chromatographic (RPLC) ... Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid Chromatography: A Quantitative ... Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid Chromatography: A Quantitative ... "Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid Chromatography: A Quantitative ...
more infohttp://mdpi.com/1422-0067/13/11/15387

IJMS  | Free Full-Text | Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid...IJMS | Free Full-Text | Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid...

... have successfully been developed for naturally occurring phenolic compounds in a reversed-phase liquid chromatographic (RPLC) ... reversed-phase liquid chromatography-mass spectrometry is considered a practically state-of-the-art technique; as reversed- ... Structure-retention and mobile phase-retention relationships for reversed-phase high-performance liquid chromatography of ... Predicting Retention Times of Naturally Occurring Phenolic Compounds in Reversed-Phase Liquid Chromatography: A Quantitative ...
more infohttps://www.mdpi.com/1422-0067/13/11/15387/htm

Reversed-phase thin-layer chromatography of homologs of Antimycin-A and related derivativesReversed-phase thin-layer chromatography of homologs of Antimycin-A and related derivatives

Reversed-phase gradient HPLC separation of the dansylated or methylated antibiotic compounds produced superior chromatographic ... Although a base-line resolution of the known four major antimycins Al, A2, A3, and A4 was readily achieved with mobile phases ... Of the numerous chromatographic systems examined, a mobile phase consisting of methanol-water (70:30) and 0.005 M ... the separation of the new antibiotic subcomponents was highly sensitive to variation in mobile phase conditions. The type and ...
more infohttps://pubs.er.usgs.gov/publication/1003129

Deformations of overloaded bands under pH-stable conditions in reversed phase chromatographyDeformations of overloaded bands under pH-stable conditions in reversed phase chromatography

Liquid chromatography, HPLC, UHPLC, Reversed phase, Preparative chromatography, Adsorption isotherm, Injection profile, Sample ... Deformations of overloaded bands under pH-stable conditions in reversed phase chromatography. Edström, Lena Uppsala University ... Journal of Chromatography A On the subject. Analytical Chemistry Search outside of DiVA. GoogleGoogle Scholar. ... 2011 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1218, no 15, 1966-1973 p.Article in ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:410063

Separation of functionalized dextrins by reversed-phase high-performance liquid chromatography - InfoscienceSeparation of functionalized dextrins by reversed-phase high-performance liquid chromatography - Infoscience

on a preparative scale by HPLC on RP-18 phases with water as the eluent. These dextrins were obtained by the transfer of a- ... reversed-phase ; of functionalized dextrins) ; functionalized dextrin sepn reversed phase HPLC; liq chromatog functionalized ... Separation of functionalized dextrins by reversed-phase high-performance liquid chromatography Andriamboavonjy, E. ; Flaschel, ... Home , Separation of functionalized dextrins by reversed-phase high-performance liquid chromatography ...
more infohttps://infoscience.epfl.ch/record/84293

SIMULTANEOUS DETERMINATION OF PROPRANOLOL HYDROCHLORIDE AND FLUNARIZINE DIHYDROCHLORIDE IN BULK AND CAPSULE USING REVERSED -...SIMULTANEOUS DETERMINATION OF PROPRANOLOL HYDROCHLORIDE AND FLUNARIZINE DIHYDROCHLORIDE IN BULK AND CAPSULE USING REVERSED -...

PHASE HIGH -PERFORMANCE THIN LAYER CHROMATOGRAPHY / DENSITOMETRY. J. Chil. Chem. Soc. [online]. 2012, vol.57, n.1, pp.1033-1035 ... as mobile phase. Densitometric scanning was performed at 267 nm. The Rf values for PRH and FNZ were found to be 0.63 and 0.48, ... SIMULTANEOUS DETERMINATION OF PROPRANOLOL HYDROCHLORIDE AND FLUNARIZINE DIHYDROCHLORIDE IN BULK AND CAPSULE USING REVERSED - ...
more infohttp://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0717-97072012000100017&lng=en&nrm=iso&tlng=en

Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the...Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the...

Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the ... Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the ... Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the ... Multidimensional Liquid Chromatography Separation of Intact Proteins by Chromatographic Focusing and Reversed Phase of the ...
more infohttps://www.mcponline.org/content/5/1/26?ijkey=7bbc4e57e81ecad054fad8792490563297059606&keytype2=tf_ipsecsha

MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY | Biochemical...MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY | Biochemical...

MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Ole Chr. ... MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ... MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ... MEASUREMENT OF FREE COENZYME A IN BIOLOGICAL EXTRACTS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ...
more infohttp://www.biochemsoctrans.org/content/9/2/192P.6

Rapid and Sensitive Reverse-phase High-performance Liquid Chromatography Method for Estimation of Ketorolac in Pharmaceuticals...Rapid and Sensitive Reverse-phase High-performance Liquid Chromatography Method for Estimation of Ketorolac in Pharmaceuticals...

Rapid and Sensitive Reverse-phase High-performance Liquid Chromatography Method for Estimation of Ketorolac in Pharmaceuticals ... Rapid and Sensitive Reverse-phase High-performance Liquid Chromatography Method for Estimation of Ketorolac in Pharmaceuticals ... Chaudhary RS, Gangwal SS, Jindal KC, Khanna S. Reversedphase high‑performance liquid chromatography of ketorolac and its ... A reliable, rapid and sensitive isocratic reverse phase high-performance liquid chromatography method has been developed and ...
more infohttps://www.ijpsonline.com/articles/rapid-and-sensitive-reversephase-highperformance-liquid-chromatography-method-for-estimation-of-ketorolac-in-pharmaceuticals-using.html

Ethylammonium Acetate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography | Springer for Research & DevelopmentEthylammonium Acetate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography | Springer for Research & Development

... and characterized a concentrated solution of EAA as a reversed phase mobile phase replacement for organic... ... Ethylammonium Acetate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography. ... and characterized a concentrated solution of EAA as a reversed phase mobile phase replacement for organic solvents like ... Column liquid chromatography Aqueous mobile phase modifier Ethylammonium acetate Water-soluble vitamins ...
more infohttps://rd.springer.com/article/10.1365/s10337-004-0460-0

TKS Publisher |   ZEOCHEM - ZEOsphere® DRPDoped Reversed Phase Chromatography GelsFaster, better and more economic than...TKS Publisher | ZEOCHEM - ZEOsphere® DRPDoped Reversed Phase Chromatography GelsFaster, better and more economic than...

Doped Reversed Phase Chromatography Gels. Faster, better and more economic than traditional separation technologies.. ... and silica gels for industrial applications as well as high performance chromatography gels for the pharmaceutical ...
more infohttp://www.teknoscienze.com/tks_article/zeochem-zeosphere-drpdoped-reversed-phase-chromatography-gelsfaster-better-and-more-economic-than-traditional-separation-technologies/

Lipoxin A4: Problems with its determination using reversed phase chromatography-tandem mass spectrometry and confirmation with...Lipoxin A4: Problems with its determination using reversed phase chromatography-tandem mass spectrometry and confirmation with...

Lipoxin A4: Problems with its determination using reversed phase chromatography-tandem mass spectrometry and confirmation with ... The determination of lipoxin A(4) has been accomplished by chromatographic separation using a C18 reversed phase column and ... The authors recommend the use of chiral chromatography in the determination of pro-resolving lipid mediators, together with ...
more infohttp://publica.fraunhofer.de/documents/N-300313.html

Influence of eluent composition on the retention factor of azoles in reversed-phase high-performance liquid chromatography,...Influence of eluent composition on the retention factor of azoles in reversed-phase high-performance liquid chromatography,...

"Influence of eluent composition on the retention factor of azoles in reversed-phase high-performance liquid chromatography, ... Influence of eluent composition on the retention factor of azoles in reversed-phase high-performance liquid chromatography. ... Influence of eluent composition on the retention factor of azoles in reversed-phase high-performance liquid chromatography. ... Influence of eluent composition on the retention factor of azoles in reversed-phase... Kurbatova, S.; Kharitonova, O. 2008-05- ...
more infohttps://www.deepdyve.com/lp/springer_journal/influence-of-eluent-composition-on-the-retention-factor-of-azoles-in-I0sk41ivcH

Profiling of ornithine lipids in bacterial extracts of Rhodobacter sphaeroides by reversed-phase liquid chromatography with...Profiling of ornithine lipids in bacterial extracts of Rhodobacter sphaeroides by reversed-phase liquid chromatography with...

Profiling of ornithine lipids in bacterial extracts of Rhodobacter sphaeroides by reversed-phase liquid chromatography with ... Profiling of ornithine lipids in bacterial extracts of Rhodobacter sphaeroides by reversed-phase liquid chromatography with ... were investigated by high-performance reversed phase liquid chromatography (RPLC) with electrospray ionization mass ...
more infohttp://www.nextbio.com/b/search/article.nb?id=26709304

Synthesis and Characterization of Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and...Synthesis and Characterization of Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and...

Synthesis and Characterization of Reversed-Phase Continuous Beds for Isocratic and Gradient Capillary Liquid Chromatography and ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:104287
  • Isabelle François UGent , Alberto Dos Santos Pereira UGent and Patrick Sandra UGent ( 2009 ) High Performance Liquid Phase Separations and Related Techniques, 34th International symposium, Abstracts . (ugent.be)
  • A validated method that is based on the use of volatile ion-pair liquid chromatography coupled to stable isotope dilution tandem mass spectrometry has been developed for the simple and accurate quantitation of underivatized amino acids in biological samples. (nih.gov)
  • SURANA, SANJAY J and NAWALE, PRAJAKTA S . SIMULTANEOUS DETERMINATION OF PROPRANOLOL HYDROCHLORIDE AND FLUNARIZINE DIHYDROCHLORIDE IN BULK AND CAPSULE USING REVERSED - PHASE HIGH -PERFORMANCE THIN LAYER CHROMATOGRAPHY / DENSITOMETRY . (scielo.cl)
  • The authors recommend the use of chiral chromatography in the determination of pro-resolving lipid mediators, together with transition area ratio and fragmentation spectra to improve selectivity for identification and quantitation purposes. (fraunhofer.de)
  • Gradient elution of polystyrene standards on reversed phase C18 columns by methanol/tetrahydrofuran or methanol/dichloromethane mixtures yielded a strange effect of the molecular weight of the sample on the specific peak are (mAUs per μg sample injected). (springer.com)
  • Despite the relatively high viscosity of EAA, plate count values averaged only about 15% less for EAA as compared to methanol using the same mobile phase composition and could be raised by either working at a temperature above ambient or reducing the flow rate. (springer.com)
  • Kharitonova, O. 2008-05-09 00:00:00 The linear dependence of the retention factor of indole, imidazole, triazole, and tetrazole derivatives on the acetonitrile content of the mobile phase was studied. (deepdyve.com)
  • Isabelle François UGent , Alberto Dos Santos Pereira UGent and Patrick Sandra UGent ( 2009 ) High Performance Liquid Phase Separations and Related Techniques, 34th International symposium, Abstracts . (ugent.be)
  • A robust companion in the field is liquid chromatography, the method used in this thesis, which is an established workhorse and a versatile tool in many different disciplines. (diva-portal.org)