Chromatography, High Pressure Liquid
Pressure
Chromatography, Thin Layer
Mass Spectrometry
Chromatography, Gel
Chromatography, Affinity
Chromatography, Ion Exchange
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Amino Acid Sequence
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Peptide Fragments
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
High Pressure Neurological Syndrome
A syndrome related to increased atmospheric pressure and characterized by tremors, nausea, dizziness, decreased motor and mental performance, and SEIZURES. This condition may occur in those who dive deeply (c. 1000 ft) usually while breathing a mixture of oxygen and helium. The condition is associated with a neuroexcitatory effect of helium.
Electrophoresis, Polyacrylamide Gel
Trypsin
Atmospheric Pressure
Arachidonate Lipoxygenases
Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .
Amino Acids
Gas Chromatography-Mass Spectrometry
Spectrophotometry, Ultraviolet
Lipoxygenase
Magnetic Resonance Spectroscopy
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Hydroxyeicosatetraenoic Acids
Cattle
Cyanogen Bromide
Rats, Inbred Strains
Substrate Specificity
Biotransformation
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Spectrometry, Mass, Fast Atom Bombardment
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Hydrogen-Ion Concentration
Solvents
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Spectrometry, Mass, Electrospray Ionization
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
Air Pressure
SRS-A
A group of LEUKOTRIENES; (LTC4; LTD4; and LTE4) that is the major mediator of BRONCHOCONSTRICTION; HYPERSENSITIVITY; and other allergic reactions. Earlier studies described a "slow-reacting substance of ANAPHYLAXIS" released from lung by cobra venom or after anaphylactic shock. The relationship between SRS-A leukotrienes was established by UV which showed the presence of the conjugated triene. (From Merck Index, 11th ed)
Neurotensin
A biologically active tridecapeptide isolated from the hypothalamus. It has been shown to induce hypotension in the rat, to stimulate contraction of guinea pig ileum and rat uterus, and to cause relaxation of rat duodenum. There is also evidence that it acts as both a peripheral and a central nervous system neurotransmitter.
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)
Stereoisomerism
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Carbohydrate Sequence
Isomerism
Oligosaccharides
Rabbits
Ionic Liquids
Affinity Labels
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Isoelectric Focusing
Liver
Brain Chemistry
Microsomes, Liver
Arachidonic Acid
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
Binding Sites
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bile
Endopeptidases
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Dogs
Hypertension
Hydroxylation
Carbon Radioisotopes
Molecular Structure
Models, Chemical
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Macromolecular Substances
Temperature
Base Sequence
Intracranial Pressure
Dose-Response Relationship, Drug
Blood Pressure Monitoring, Ambulatory
Tandem Mass Spectrometry
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Protein Binding
Kidney
Cloning, Molecular
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Phospholipids
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Chemistry
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Immune Sera
Chemical Phenomena
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Binding, Competitive
Erythrocytes
Photobacterium
Chromatography, DEAE-Cellulose
Microsomes
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Venous Pressure
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Structure-Activity Relationship
Phosphorylation
Cells, Cultured
Cricetinae
Isoenzymes
Tyrosine
Glycopeptides
Adenosine Triphosphate
Diamond
Sequence Homology, Amino Acid
Arterial Pressure
Ventricular Pressure
The pressure within a CARDIAC VENTRICLE. Ventricular pressure waveforms can be measured in the beating heart by catheterization or estimated using imaging techniques (e.g., DOPPLER ECHOCARDIOGRAPHY). The information is useful in evaluating the function of the MYOCARDIUM; CARDIAC VALVES; and PERICARDIUM, particularly with simultaneous measurement of other (e.g., aortic or atrial) pressures.
Mutation
Chromatography, Reverse-Phase
Uranus
Chromatography, Agarose
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Water
Rats, Sprague-Dawley
Hemodynamics
Freeze Substitution
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Neptune
Central Venous Pressure
Pulmonary Wedge Pressure
Antihypertensive Agents
Drugs used in the treatment of acute or chronic vascular HYPERTENSION regardless of pharmacological mechanism. Among the antihypertensive agents are DIURETICS; (especially DIURETICS, THIAZIDE); ADRENERGIC BETA-ANTAGONISTS; ADRENERGIC ALPHA-ANTAGONISTS; ANGIOTENSIN-CONVERTING ENZYME INHIBITORS; CALCIUM CHANNEL BLOCKERS; GANGLIONIC BLOCKERS; and VASODILATOR AGENTS.
Oxygen
Thermodynamics
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Germanium
Chromatography, Supercritical Fluid
Osmotic Pressure
Helium
Helium. A noble gas with the atomic symbol He, atomic number 2, and atomic weight 4.003. It is a colorless, odorless, tasteless gas that is not combustible and does not support combustion. It was first detected in the sun and is now obtained from natural gas. Medically it is used as a diluent for other gases, being especially useful with oxygen in the treatment of certain cases of respiratory obstruction, and as a vehicle for general anesthetics. (Dorland, 27th ed)
Synchrotrons
Chromatography, Paper
Solubility
Vascular Resistance
Protein Denaturation
Food Handling
Calibration
Hyperbaric Oxygenation
The therapeutic intermittent administration of oxygen in a chamber at greater than sea-level atmospheric pressures (three atmospheres). It is considered effective treatment for air and gas embolisms, smoke inhalation, acute carbon monoxide poisoning, caisson disease, clostridial gangrene, etc. (From Segen, Dictionary of Modern Medicine, 1992). The list of treatment modalities includes stroke.
Carbon Dioxide
Models, Molecular
Enzyme Activation
Evolution, Planetary
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Pulse
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Norepinephrine
Precursor of epinephrine that is secreted by the adrenal medulla and is a widespread central and autonomic neurotransmitter. Norepinephrine is the principal transmitter of most postganglionic sympathetic fibers and of the diffuse projection system in the brain arising from the locus ceruleus. It is also found in plants and is used pharmacologically as a sympathomimetic.
Phycomyces
Reference Standards
Chemical Fractionation
Iron Compounds
Rats, Wistar
Spectrophotometry
Cerebrospinal Fluid Pressure
Carbohydrates
Positive-Pressure Respiration
Prospective Studies
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Sensitivity and Specificity
Fatty Acids
An investigation into the binding of the carcinogen 15,16-dihydro-11-methylcyclopenta[a]phenanthren-17-one to DNA in vitro. (1/27865)
After metabolic activation the carcinogen 15,16-dihydro-11-[3H]methylcyclopenta[a]phenanthren-17-one binds to DNA in vitro, and this binding is prevented by 7,8-benzoflavone. Radioactivity cannot be removed from the DNA with organic solvents or by chromatography on Sephadex G-50, even after heat denaturation of the DNA. Enzymatic hydrolysis yields radioactive fractions, which elute from a column of Sephadex LH-20 immediately after the natural nucleosides. At least two species of reactive metabolites are involved in this bending, those with a half-life of a few hr and others with greater stability. After extraction from the aqueous incubation mixture, they could be detected in discrete polar fractions from separations of the complex metabolite mixture by high-pressure liquid chromatography. Their ability to bind to DNA decreased with time at ambient temperature, and they were rapidly deactivated by acid. 7,8-Benzolflavone acted by suppressing the formation of polar metabolites derived from enzymatic oxidation of the aromatic double bonds. The inhibitor had no effect on the enzymes hydroxylating saturated carbon; hence it is unlikely that metabolism of the methyl group is important in conversion of this carcinogen to its proximate form, although the presence of the 11-methyl group is essential for carcinogenic activity in this series. (+info)The direct spectrophotometric observation of benzo(a)pyrene phenol formation by liver microsomes. (2/27865)
Optical spectral repetitive scan analysis during the oxidative metabolism of benzo(a)pyrene by liver microsomal suspensions reveals the time-dependent formation of an intermediate(s) of which the visible spectra resemble those of several benzo(a)pyrene phenols. Liver microsomes from 3-methylcholanthrene-treated rats showed a greater rate of formation of the phenols than did microsomes from control animals; the rate of formation catalyzed by liver microsomes from phenobarbital-pretreated rats was intermediate. When 3-hydroxybenzo(a)pyrene was used as a standard for comparison of activity, the rates of formation of phenols were compared when measured by fluorometric, spectrophotometric, or high-pressure liquid chromatographic analytical techniques. An epoxide hydrase inhibitor, 1,1,1-trichloropropene-2,3-oxide, enhanced phenol formation regardless of the source of liver microsomes, and 7,8-benzoflavone inhibited control and 3-methylcholanthrene-induced microsomal metabolism of benzo(a)pyrene, 7,8-Benzoflavone did not effect benzo(a)pyrene metabolism by liver microsomes from phenobarbital-pretreated rats. The effect of inhibitors on the spectrophotometric assay correlates well with the results obtained from benzo(a)pyrene metabolite analysis using high-pressure liquid chromatography. (+info)A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila. (3/27865)
Recently, we reported the identification of a 55-kDa polypeptide (p55) from Tetrahymena macronuclei as a catalytic subunit of a transcription-associated histone acetyltransferase (HAT A). Extensive homology between p55 and Gcn5p, a component of the SAGA and ADA transcriptional coactivator complexes in budding yeast, suggests an immediate link between the regulation of chromatin structure and transcriptional output. Here we report the characterization of a second transcription-associated HAT activity from Tetrahymena macronuclei. This novel activity is distinct from complexes containing p55 and putative ciliate SAGA and ADA components and shares several characteristics with NuA4 (for nucleosomal H2A/H4), a 1.8-MDa, Gcn5p-independent HAT complex recently described in yeast. A key feature of both the NuA4 and Tetrahymena activities is their acetylation site specificity for lysines 5, 8, 12, and 16 of H4 and lysines 5 and 9 of H2A in nucleosomal substrates, patterns that are distinct from those of known Gcn5p family members. Moreover, like NuA4, the Tetrahymena activity is capable of activating transcription from nucleosomal templates in vitro in an acetyl coenzyme A-dependent fashion. Unlike NuA4, however, sucrose gradient analyses of the ciliate enzyme, following sequential denaturation and renaturation, estimate the molecular size of the catalytically active subunit to be approximately 80 kDa, consistent with the notion that a single polypeptide or a stable subcomplex is sufficient for this H2A/H4 nucleosomal HAT activity. Together, these data document the importance of this novel HAT activity for transcriptional activation from chromatin templates and suggest that a second catalytic HAT subunit, in addition to p55/Gcn5p, is conserved between yeast and Tetrahymena. (+info)Isolation and complete covalent structure of liver microsomal paraoxonase. (4/27865)
Paraoxonase (PON1) is a serum esterase exclusively associated with high-density lipoproteins; it might confer protection against coronary artery disease by destroying pro-inflammatory oxidized lipids in oxidized low-density lipoproteins. Here I show that rabbit liver microsomes contain a PON analogue (MsPON) and report the isolation and complete covalent structure of MsPON. In detergent-solubilized microsomes, MsPON co-purifies with the microsomal triacylglycerol transfer protein (MTP) complex. MsPON was separated from the complex and purified to homogeneity under non-denaturing conditions. Automated sequence analysis of intact MsPON and peptides obtained from enzymic and chemical cleavages led to the elucidation of the complete covalent structure of MsPON. The protein is a single polypeptide consisting of 350 residues. The sequence of rabbit liver microsomal MsPON is 60% identical with that of rabbit serum PON1, and 84% identical with the sequence predicted by a human cDNA of unknown function, designated PON3. MsPON has a hydrophobic segment at the N-terminus that might serve to anchor the protein to the microsomal membrane or to the MTP complex. Unlike in the serum enzyme, two potential N-glycan acceptor sites in MsPON are not glycosylated. An absence of N-glycans was also indicated in the rabbit liver MTP. MsPON has a single free cysteine residue at position 38 and a disulphide bond between Cys-279 and Cys-348. The microsomal enzyme lacks three residues at the N-terminus that are present in the serum protein. MsPON lacks four residues at the C-terminus that are present in the rabbit serum protein but absent from human serum PON1. On the basis of the observation that MsPON displays a high degree of similarity with serum PON1, it is proposed that MsPON might have a function related to that of PON1 in serum high-density lipoprotein complexes. (+info)Simultaneous antisense inhibition of two starch-synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin. (5/27865)
A chimaeric antisense construct was used to reduce the activities of the two major starch-synthase isoforms in potato tubers simultaneously. A range of reductions in total starch-synthase activities were found in the resulting transgenic plants, up to a maximum of 90% inhibition. The reduction in starch-synthase activity had a profound effect on the starch granules, which became extremely distorted in appearance compared with the control lines. Analysis of the starch indicated that the amounts produced in the tubers, and the amylose content of the starch, were not affected by the reduction in activity. In order to understand why the starch granules were distorted, amylopectin was isolated and the constituent chain lengths analysed. This indicated that the amylopectin was very different to that of the control. It contained more chains of fewer than 15 glucose units in length, and fewer of between 15 and 80 glucose units. In addition, the amylopectin contained more very long chains. Amylopectin from plants repressed in just one of the activities of the two starch-synthase isoforms, which we have reported upon previously, were also analysed. Using a technique different to that used previously we show that both isoforms also affect the amylopectin, but in a way that is different to when both isoforms are repressed together. (+info)Purinogen is not an endogenous substrate used in endothelial cells during substrate deprivation. (6/27865)
Porcine aortic endothelial cells (PAEC) are known to be metabolically robust. They are capable of surviving extended periods of complete lack of exogenous substrate, and purine release has been shown to be significantly up-regulated. The endogenous substrates used during substrate deprivation, as well as the sources responsible for the increased purine release, have not been completely identified. We tested the possibility that a phosphoglyceroyl-ATP-containing polymer, purinogen, might support PAEC hibernation induced by lack of exogenous substrate. This involved isolation of the acid-insoluble fraction of PAEC, which was presumed to contain purinogen, and analysis by HPLC and 31P NMR. No evidence supporting the presence of triphosphate-containing compounds (purinogen) was found. Similar results were obtained in the rat heart. The majority of the products in the acid-insoluble, alkaline-treated fraction were identified as RNA degradation products (2'- and 3'-nucleoside monophosphates). A [14C]adenosine labelling experiment showed that incorporation of adenosine into the acid-insoluble fraction was almost completely prevented after inhibition of RNA synthesis with actinomycin D. Furthermore, RNA isolated from PAEC and subsequently treated with alkali showed a profile that was almost identical with the HPLC profile of the acid-insoluble fraction. Finally, substrate-free incubation of the cells did not quantitatively or qualitatively influence the distribution of acid-insoluble derivatives. We conclude that PAEC survival during the absence of exogenous substrate is not supported by purinogen but rather by some other, yet-to-be-identified, endogenous substrate. (+info)Accumulation of astaxanthin all-E, 9Z and 13Z geometrical isomers and 3 and 3' RS optical isomers in rainbow trout (Oncorhynchus mykiss) is selective. (7/27865)
Concentrations of all-E-, 9Z- and 13Z- geometrical and (3R,3'R), (3R, 3'S) and (3S,3'S) optical isomers of astaxanthin were determined in rainbow trout liver, gut tissues, kidney, skin and blood plasma to evaluate their body distribution. Two cold-pelleted diets containing predominantly all-E-astaxanthin (36.9 mg/kg astaxanthin, 97% all-E-, 0.4% 9Z-, 1.5% 13Z-astaxanthin, and 1.1% other isomers, respectively) or a mixture of all-E- and Z-astaxanthins (35.4 mg/kg astaxanthin, 64% all-E-, 18.7% 9Z-, 12.3% 13Z-astaxanthin, and 2.0% other isomers, respectively), were fed to duplicate groups of trout for 69 d. Individual E/Z isomers were identified by VIS- and 1H-NMR-spectrometry, and quantified by high-performance liquid chromatography. Significantly higher total carotenoid concentration was observed in plasma of trout fed diets with all-E-astaxanthin (P < 0.05). The relative E/Z-isomer concentrations of plasma, skin and kidney were not significantly different among groups, whereas all-E-astaxanthin was higher in intestinal tissues and 13Z-astaxanthin was lower in liver of trout fed all-E-astaxanthin (P < 0.05). The relative amount of hepatic 13Z-astaxanthin (39-49% of total astaxanthin) was higher than in all other samples (P < 0.05). Synthetic, optically inactive astaxanthin was used in all experiments, and the determined dietary ratio between the 3R,3'R:3R, 3'S (meso):3S,3'S optical isomers was 25.3:49.6:25.1. The distribution of R/S-astaxanthin isomers in feces, blood, liver and fillet was similar to that in the diets. The ratio between (3S,3'S)- and (3R,3'R)-astaxanthin in the skin and posterior kidney was ca. 2:1 and 3:1, respectively, regardless of dietary E/Z-astaxanthin composition. The results show that geometrical and optical isomers of astaxanthin are distributed selectively in different tissues of rainbow trout. (+info)Daidzein and genistein glucuronides in vitro are weakly estrogenic and activate human natural killer cells at nutritionally relevant concentrations. (8/27865)
Daidzein and genistein glucuronides (DG and GG), major isoflavone metabolites, may be partly responsible for biological effects of isoflavones, such as estrogen receptor binding and natural killer cell (NK) activation or inhibition. DG and GG were synthesized using 3-methylcholanthrene-induced rat liver microsomes. The Km and Vmax for daidzein and genistein were 9.0 and 7.7 micromol/L, and 0.7 and 1.6 micromol/(mg protein. min), respectively. The absence of ultraviolet absorbance maxima shifts in the presence of sodium acetate confirmed that the synthesized products were 7-O-glucuronides. DG and GG were further purified by a Sephadex LH-20 column. DG and GG competed with the binding of 17beta-(3H) estradiol to estrogen receptors of B6D2F1 mouse uterine cytosol. The concentrations required for 50% displacement of 17beta-(3H) estradiol (CB50) were: 17beta-estradiol, 1.34 nmol/L; diethylstilbestrol, 1.46 nmol/L; daidzein, 1.6 micromol/L; DG, 14.7 micromol/L; genistein, 0.154 micromol/L; GG, 7.27 micromol/L. In human peripheral blood NK cells, genistein at <0.5 micromol/L and DG and GG at 0.1-10 micromol/L enhanced NK cell-mediated K562 cancer cell killing significantly (P < 0.05). At > 0.5 micromol/L, genistein inhibited NK cytotoxicity significantly (P < 0.05). The glucuronides only inhibited NK cytotoxicity at 50 micromol/L. Isoflavones, and especially the isoflavone glucuronides, enhanced activation of NK cells by interleukin-2 (IL-2), additively. At physiological concentrations, DG and GG were weakly estrogenic, and they activated human NK cells in nutritionally relevant concentrations in vitro, probably at a site different from IL-2 action. (+info)
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Enantioselective high-performance liquid chromatography analysis of oxygenated polyunsaturated fatty acids. - Fungal Diagnostic...
Reverse Phase High Performance Liquid Chromatography rpHPLC
Liquid-chromatographic determination of nicotine and cotinine in urine from passive smokers: comparison with gas chromatography...
Determination of Drugs by Online Column-Switching Liquid Chromatography | AVESİS
Potassium dihydrogen phosphate 7778-77-0, China Potassium dihydrogen phosphate 7778-77-0 Manufacturers, China Potassium...
Chromatography | Free Full-Text | Simultaneous High Performance Liquid Chromatography Assay of Pentoxifylline, Mupirocin,...
澳門大學學者庫(UM): Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid...
Sensitive high-performance liquid chromatographic method for profiling phytoestrogens using coulometric electrode array...
Low pressure liquid chromatography system Page 1 of 0 | GALAK Chromatography
Rapid ultra-performance liquid chromatography assay of losartan potassium in bulk and formulations - uomeprints
High-Performance Liquid Chromatographic Analysis of Bilirubin and Biliverdin from Jaundiced Broilers
GE AKTA fast protein liquid chromatography system at Universidad Central del Caribe
Tosoh to Increase Production Capacity of Toyopearl Liquid Chromatography Separation and Purification Media - Pharmaceutical...
China High Performance Liquid Chromatography, Spectrophotometer, HPLC (RAY-230 HPLC) - China High Performance Liquid...
High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins ...
Abstract 14919: A Novel and Validated Mass Spectrometry Assay for Characterizing in vitro Fragmentation of BNP and a Method to...
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Asian Journal of Research in Chemistry
5μm TSKgel® ODS-100V Columns - TSKgel® Silica-based Reversed-Phase HPLC Columns | Sigma-Aldrich
High Performance Liquid Chromatographic Determination Of α-keto Acids From A Pharmaceutical Preparation (Tablet) Using 4-Nitro...
High pressure liquid chromatography development services
Development and Validation of HPLC Method for Estimation of Pharmaceutical Drug and its Stability Studies in Simulated...
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denaturing high performance liquid chromatography的概述|专业知识背景|拓展阅读-小狗文献
Reversed-Phase Liquid Chromatographic Separation and Determination of Ni(II), Cu(II), Pd(II), and Ag(I) Using 2...
Determination of the triacylglycerol fraction in fish oil by comprehensive liquid chromatography techniques with the support of...
chromatography-systems-liquid-packed-column-high-pressure-bonded-phase
Purification of the enhancing factor from mouse intestines - Publications of the IAS Fellows
Determination of aldehydes in rainwater using micro-solid-phase extraction and high-performance liquid chromatography |...
High-performance liquid chromatographic method for routine determination of vitamins A and E and β-carotene in plasma |...
Advanced Ultra-High Performance Liquid Chromatography Systems Enhance Lab Productivity - Feb 27, 2018
DNA methylation in mycobacteria: Absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences - [email protected]
Liquid Chromatography Systems
BioLogic™ Low-Pressure Liquid Chromatography Systems | Life Science Research | Bio-Rad
High performance liquid chromatography | Cram
Ron Lauback
"High-Pressure Liquid Chromatography". Journal of Liquid Chromatography & Related Technologies; Journal of Pharmaceutical ... examples: "High-Pressure Liquid Chromatography Assay for Dane Salt Potassium(-)-N-(1-Methoxycarbonylpropene-2yl)-p- ... Journal of Liquid Chromatography & Related Technologies, Volume 5, Issue 7 1982). "Specific High-Performance Liquid ... "High-Performance Liquid Chromatographic". Journal of Liquid Chromatography & Related Technologies. 7 (6): 1243-1265. doi: ...
Theanine
"Analysis of derivatized and underivatized theanine enantiomers by high-performance liquid chromatography/atmospheric pressure ... Finger, Andreas; Kuhr, Susanne; Engelhardt, Ulrich (1992). "Chromatography of tea constituents". Journal of Chromatography. 624 ... which has high theanine content.[10] Theanine is substantially present in black, green, and white teas from Camellia sinensis ... or urinalysis in rats fed high doses of theanine for 13 weeks.[42] Large studies in humans have not been undertaken; however, ...
List of Acacia species known to contain psychoactive alkaloids
"Reversed-phase high-pressure liquid chromatography of some tryptamine derivatives." Journal of Chromatography 157:365-370. ...
Norcocaine
Evans MA, Morarity T (January 1980). "Analysis of cocaine and cocaine metabolites by high pressure liquid chromatography". ... The local anesthetic potential of norcocaine has been shown to be higher than that of cocaine, however cocaine continues to be ...
Richard Zare
... subpicogram detection of aflatoxins using high-pressure liquid chromatography". Science. 196 (4297): 1439-1441. Bibcode:1977Sci ... and for high performance liquid chromatography (HPLC) Zare is also involved in the development of desorption electrospray ... Snyder, KL; Zare, RN (1 July 2003). "Cavity ring-down spectroscopy as a detector for liquid chromatography". Analytical ... This opened up the potential for a wide variety of fluid applications, including the detection of single molecules in liquids ...
T-2 mycotoxin
For the isolation of the compound high pressure liquid chromatography is commonly used (HPLC). In the Fusarium species, ... It was also found that jungle bees in this area fly collectively in great numbers, at altitudes too high to be easily seen, ... This includes using activated charcoal, which has a high binding capacity of 0.48 mg of T-2 mycotoxin to 1 mg of charcoal. For ... Research has shown that a high dose of T-2 delays the ovulation due to a delayed follicle maturation. This possibly retards the ...
Bilirubin diglucuronide
Assay by high-pressure liquid chromatography". Hepatology. 1 (6): 622-7. doi:10.1002/hep.1840010610. PMID 6796486. Lengyel, G ...
Crenarchaeol
GDGTs such as crenarchaeol can be analyzed using high-performance liquid chromatography/atmospheric pressure chemical ... "Analytical methodology for TEX86 paleothermometry by high-performance liquid chromatography/atmospheric pressure chemical ... Most GDGTs degrade at between 240 and 300 °C and so are not found in rocks that have undergone heating to temperatures higher ... It has been proposed that GDGT membrane lipids are an adaptation to the high temperatures present in the environments that are ...
Acetone peroxide
"Trace analysis of peroxide explosives by high performance liquid chromatography-atmospheric pressure chemical ionization-tandem ... high performance liquid chromatography/mass spectrometry (HPLC/MS),[19][20][21][22][23] and HPLC with post-column ... High / moderate when wet. Detonation velocity. 5300 m/s at maximum density (1.18 g/cm3), about 2500 - 3000 m/s near 0.5 g/cm3. ... Several methods can be used for trace analysis of TATP,[13] including gas chromatography/mass spectrometry (GC/MS),[14][15][16] ...
Boldine
"Simultaneous Determination of Four Alkaloids in Lindera aggregata by Ultra-High-Pressure Liquid Chromatography-Tandem Mass ... Spectrometry". Journal of Chromatography A. 1212 (1-2): 76-81. doi:10.1016/j.chroma.2008.10.017. PMID 18951552. Zhang, A.; ...
Theanine
"Analysis of derivatized and underivatized theanine enantiomers by high-performance liquid chromatography/atmospheric pressure ... Finger, Andreas; Kuhr, Susanne; Engelhardt, Ulrich (1992). "Chromatography of tea constituents". Journal of Chromatography. 624 ... Natural Standard rates the evidence to support the usage of theanine for anxiety reduction, blood pressure control, and mood ... which has high theanine content. Theanine is substantially present in black, green, and white teas from Camellia sinensis in ...
Ketamine
Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry. J. ... Low blood pressure is harmful in people with severe head injury, and ketamine is least likely to cause low blood pressure and ... Liver toxicity of ketamine also involves higher doses and repeated administration. In a group of chronic high dose ketamine ... At sufficiently high doses, users may experience what is called the "K-hole", a state of dissociation with visual and auditory ...
Theanine
"Analysis of derivatized and underivatized theanine enantiomers by high-performance liquid chromatography/atmospheric pressure ... Finger, Andreas; Kuhr, Susanne; Engelhardt, Ulrich (1992). "Chromatography of tea constituents". Journal of Chromatography. 624 ... Studies on test rats have shown even repeated, extremely high doses of theanine cause little to no harmful psychological or ... which has high theanine content.[10] Theanine is substantially present in black, green, and white teas from Camellia sinensis ...
Hexadimethrine bromide
... and high pressure liquid chromatography". Biochemistry. 17 (11): 2124-2133. doi:10.1021/bi00604a016. ISSN 0006-2960. PMID ...
Capillary electrochromatography
High-performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with ... Chromatography Electrophoresis High-performance liquid chromatography Capillary electrophoresis Electrochromatography Dittmann ... Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and ... Firstly, the pressure driven flow rate across a column depends directly on the square of the particle diameter and inversely on ...
Robert Kennedy (chemist)
... and ultra-high pressure liquid chromatography. He has been an Lilly Analytical Research Fellow, Alfred P. Sloan Fellow, NSF ... Robert (Bob) Kennedy (born 1962) is an American chemist specializing in bioanalytical chemistry including liquid chromatography ... He is considered a leader in the field of analytical chemistry, and an expert in endocrinology, neurochemistry, and high- ... Martin Medal (2019) Ralph N. Adams Award in Bioanalyical Chemistry (2016) ACS Award in Chromatography (2017) CASSS Award for ...
Phenypressin
... ion-exchange chromatography, and high pressure liquid chromatography. Phenypressin differs from the common hormone, arginine ... Secondly, it can also cause vasoconstriction, increasing the blood pressure. This neurohypophysial hormone was identified and ...
Direct analysis in real time
Eluate from a high-pressure liquid chromatograph (HPLC) can be also introduced to the reaction zone of the DART source and ... Gas chromatography has been carried out by coupling gas chromatography columns directly into the DART gas stream through a ... Liquids are typically analyzed by dipping an object (such as a glass rod) into the liquid sample and then presenting it to the ... It does not need a specific sample preparation, so it can be used for the analysis of solid, liquid and gaseous samples in ...
Vitamin B12 total synthesis
It was fortunate indeed that, just around that time, the technique of high pressure liquid chromatography (HPLC) had been ... Huber, J. F. K. (1969). "High Efficiency, High Speed Liquid Chromatography in Columns". Journal of Chromatographic Science. 7 ( ... Using cadmium(II) as the coordinating metal ion led to a very high diastereoselectivity:44-46 in favor of the natural A/D-trans ...
Arginine
It did not lower systolic blood pressure or improve weight at birth. Both liquid chromatography and liquid chromatography/mass ... including women with high blood pressure as part of pre-eclampsia. ... A meta-analysis showed that L-arginine reduces blood pressure with pooled estimates of 5.4 mmHg for systolic blood pressure and ... Forbes SC, Bell GJ (June 2011). "The acute effects of a low and high dose of oral L-arginine supplementation in young active ...
Alpha-Amanitin
More recently, the use of high-pressure liquid chromatography (HPLC) has become the preferred method, which allows for better ... Journal of Chromatography A. 8th International Symposium on High Performance Capillary Electrophoresis Part I. 744 (1-2): 167- ... Early methods to detect alpha-amanitin included thin-layer chromatography (TLC). In most solvent systems used in TLC, alpha- ... Also, amanitin-based antibody-drug conjugates using an anti-Her2 antibody such as trastuzumab showed high antitumor activity in ...
Materials MASINT
Liquid chromatography-mass spectrometry (LC-MS), and high performance (also called high pressure) liquid chromatography (HPLC ... Too high a false positive rate, without an operator that understands the context, can cause real alarms to be ignored. In an ... This vehicle can keep up with moving troops, detecting liquid and vapor hazards. Newer versions, like the M1135 Nuclear, ... Materials MASINT involves the collection, processing, and analysis of gas, liquid, or solid samples, is critical in defense ...
Acetone peroxide
"Trace analysis of peroxide explosives by high performance liquid chromatography-atmospheric pressure chemical ionization-tandem ... high performance liquid chromatography/mass spectrometry (HPLC/MS), and HPLC with post-column derivitization. Acetone peroxide ... DADP has lower molecular weight and higher vapor pressure. This means that DADP is more prone to sublimation than TATP. Several ... "Analysis of triacetone triperoxide by gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry by ...
Insulin (medication)
... by the mid-1970s thanks to high-pressure liquid chromatography (HPLC) methods. Minor allergic reactions still occur ... The basic appeal of hypoglycemic agents by mouth is that most people would prefer a pill or an oral liquid to an injection. ... It is also used along with glucose to treat high blood potassium levels. Typically it is given by injection under the skin, but ... Insulin is a protein hormone that is used as a medication to treat high blood glucose. This includes in diabetes mellitus type ...
Mercury contamination in California waterways
The most common method of speciation for the removal, is gas chromatography or high-pressure liquid chromatography paired with ... Some of these methods that are continuously being researched and sampled are the use of high-performance liquid chromatography ... Then these water canons which were called monitors it would shoot a very high pressure stream that would break apart hillsides ... The environments that methylmercury levels are most commonly found at high levels, or future high risks are wetlands, newly ...
Chromatography
Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is ... Fast protein liquid chromatography. Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often ... referred to as high-performance liquid chromatography (HPLC). In HPLC the sample is forced by a liquid at high pressure (the ... paper chromatography, gas chromatography, and what would become known as high-performance liquid chromatography. Since then, ...
Ion chromatography
High performance liquid chromatography. Aqueous Normal Phase Chromatography. Size exclusion chromatography. Micellar liquid ... Incorporation of high temperature and pressure allows a significant increase in the efficiency of ion chromatography, along ... "The art and science of forming packed analytical high-performance liquid chromatography columns". Journal of Chromatography A. ... Svec, Frantisek.; Frechet, Jean M. J. (1992). "Continuous rods of macroporous polymer as high-performance liquid chromatography ...
Estonian Americans
... high pressure liquid chromatography, and the formulation of the Rootare-Prenzlow Equation. In journalism, Edmund S. Valtman, a ... The pressures of industrialization drove numerous Estonian peasants to emigrate to the United States continuing until the ...
James Jorgenson
Chem., 62, 978-984 (1990). Ultra High Pressure Reversed Phase Liquid Chromatography in Packed Capillary Columns, J.E. MacNair, ... The group currently focuses on ultra-high pressure capillary liquid chromatography combined with mass spectrometry (MS). ... and the invention of ultra-high pressure liquid chromatography. His publications in separation methods have been extensively ... "Two-Dimensional High-Performance Liquid Chromatography/Capillary Electrophoresis", J.W. Jorgenson and M.M. Bushey, U.S. Patent ...
Monolithic HPLC column
In order to withstand the pressure, a new field of chromatography came into being: UHPLC or UPLC- ultra high pressure liquid ... A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal ... 17). "SilicaRODTM A new challenge in fast high-performance liquid chromatography separations." Trends in Analytical Chemistry, ... Perfusion chromatography showed, for the first time, that chromatography media could support high flow rates without ...
Hypervitaminosis A
... from retinol in serum and other tissues and quantified with the use of methods such as high-performance liquid chromatography.[ ... Increased intracranial pressure manifesting as cerebral edema, papilledema, and headache[4] (may be referred to as Idiopathic ... Cod liver oil is particularly high in vitamin A.. *Medications - at high doses of vitamin A - are often used on long-term basis ... Diet - liver is high in vitamin A. The liver of certain animals - including the polar bear, bearded seal,[24][25] walrus,[26] ...
Materials science
At high temperatures used to prepare glass, the material is a viscous liquid. The structure of glass forms into an amorphous ... It defines macroscopic variables, such as internal energy, entropy, and pressure, that partly describe a body of matter or ... chromatography, thermal analysis, electron microscope analysis, etc. Structure is studied at various levels, as detailed below ... Due to its higher electron mobility and saturation velocity compared to silicon, it is a material of choice for high-speed ...
Lactose intolerance
Stool sugar chromatography[edit]. Chromatography can be used to separate and identify undigested sugars present in faeces. ... This lead to higher frequencies of these countries' people having a tolerance to lactose. Conversely, regions of the south, ... Some barbecue sauces and liquid cheeses used in fast-food restaurants may also contain lactose. Lactose is often used as the ... The unabsorbed sugars and fermentation products also raise the osmotic pressure of the colon, causing an increased flow of ...
Evaporation
... to proceed more quickly with higher flow rates between the gaseous and liquid phase and in liquids with higher vapor pressure. ... to dry or concentrate samples is a common preparatory step for many laboratory analyses such as spectroscopy and chromatography ... If a liquid is heated, when the vapor pressure reaches the ambient pressure the liquid will boil. ... Many of the molecules return to the liquid, with returning molecules becoming more frequent as the density and pressure of the ...
Kava
... using high performance thin layer chromatography (HPTLC)". Food Chemistry. 151: 554-560. doi:10.1016/j.foodchem.2013.11.120. ... With kava's increasing popularity, bars serving the plant in its liquid state are beginning to open up outside of the South ... The pressure prompted Germany to reconsider the evidence base for banning kava-based pharmaceutical products.[99] On 10 June ... Journal of High Resolution Chromatography. 20 (10): 555-559. doi:10.1002/jhrc.1240201007.. ...
Chemistry
... it occurs in a more ordered phase like liquid or solid as is the case with water (H2O); a liquid at room temperature because ... That is, substances with a higher Ka are more likely to donate hydrogen ions in chemical reactions than those with lower Ka ... For example, there are three phases of solid iron (alpha, gamma, and delta) that vary based on temperature and pressure. A ... They can be analyzed using the tools of chemical analysis, e.g. spectroscopy and chromatography. Scientists engaged in chemical ...
Methylenedioxypyrovalerone
MDPV may be quantified in blood, plasma or urine by gas chromatography-mass spectrometry or liquid chromatography-mass ... High doses have been observed to cause intense, prolonged panic attacks in stimulant-intolerant users, and there are anecdotal ... in these patients can cause an unopposed peripheral alpha-adrenergic effect with a dangerous paradoxical rise in blood pressure ... by gas chromatography/mass spectrometry and liquid chromatography/quadrupole time-of-flight mass spectrometry". Rapid ...
Pentaerythritol tetranitrate
The most common use of PETN is as an explosive with high brisance. It is more difficult to detonate than primary explosives, so ... PETN forms eutectic mixtures with some liquid or molten aromatic nitro compounds, e.g. trinitrotoluene (TNT) or tetryl. Due to ... The Los Angeles Times noted in November 2010 that PETN's low vapor pressure makes it difficult for bomb-sniffing dogs to detect ... in plasma using gas chromatography-mass spectrometry.[49] ... specifically in the high explosive Mine shell.[citation needed] ...
Peptide
"Liquid chromatography-mass spectrometry: potential in forensic and clinical toxicology". Journal of Chromatography B. 733 (1-2 ... Lactotripeptides - Lactotripeptides might reduce blood pressure,[20][21][22] although the evidence is mixed.[23] ... These function, typically in higher organisms, as hormones and signaling molecules. Some organisms produce peptides as ... Xu JY, Qin LQ, Wang PY, Li W, Chang C; Qin; Wang; Li; Chang (October 2008). "Effect of milk tripeptides on blood pressure: a ...
Ytterbium
As ytterbium(III) oxide has a significantly higher emissivity in the infrared range than magnesium oxide, a higher radiant ... although the exact transformation temperature depends on the pressure and stress.[7] The beta allotrope (6.966 g/cm3) exists at ... "Optimization of Separation of Ytterbium and Lutetium by Displacement Complexing Chromatography". Russian Journal of Applied ... ytterbium has the smallest liquid range of all the metals.[6] ... XIV: High Density-High Performance Decoy Flare Compositions ...
Index of chemistry articles
Emil Fischer Hermann Staudinger heterocyclic compound Hexane Hiddenite Hideki Shirakawa High performance liquid chromatography ... Valence Van der Waals radius Van der Waals force Vanadium Vapor pressure Vapour pressure Vermilion Victor Grignard Viktor Meyer ... of sound Sperrylite Spinel Spodumene stable isotope standard temperature and pressure Standard Ambient Temperature and Pressure ... p-block paint Palagonite Palladium Parabens Partial pressure Pascal (unit) Paul Berg Paul D. Boyer Paul J. Crutzen Paul J. ...
Germanium
The reflectivity has advantages over silicon in neutron and high energy X-ray applications.[75] Crystals of high purity ... 3) were found to be less hazardous and may be used as a liquid substitute for toxic germane gas in semiconductor applications. ... At pressures above 120 kbar, it becomes the allotrope β-germanium with the same structure as β-tin.[28] Like silicon, gallium, ... Sol-Gel Hybrid Organic-Inorganic Coatings for Capillary Microextraction and Gas Chromatography". Anal. Chem. 79 (24): 9441-9451 ...
Herbalism
Extracts include liquid extracts, dry extracts, and nebulisates. Liquid extracts are liquids with a lower ethanol percentage ... For example, dangerously low blood pressure may result from the combination of an herbal remedy that lowers blood pressure ... Maceration is the cold infusion of plants with high mucilage-content, such as sage or thyme. To make macerates, plants are ... For this reason, thin layer chromatography is sometimes used by growers to assess the content of their products before use. ...
List of plasma physics articles
Low-pressure discharge. *Luminescent solar concentrator. *Lundquist number. *Luttinger liquid. MEdit. *Madison Symmetric Torus ... MARAUDER, acronym of Magnetically Accelerated Ring to Achieve Ultra-high Directed Energy and Radiation ... Chromatography detector. *Chromo-Weibel instability. *Classical-map hypernetted-chain method. *Cnoidal wave ...
Melamine
The above reaction can be carried out by either of two methods: catalyzed gas-phase production or high pressure liquid-phase ... both based on liquid chromatography - mass spectrometry (LC/MS) detection after hydrophilic interaction liquid chromatography ( ... and high performance liquid chromatography. The result of the analysis showed that the calculus was composed of melamine and ... the FDA began using a high performance liquid chromatography test to determine the melamine, ammeline, ammelide, and cyanuric ...
James Jorgenson
Ultra High Pressure Reversed Phase Liquid Chromatography in Packed Capillary Columns, J.E. MacNair, K.C. Lewis, and J.W. ... The group currently focuses on ultra-high pressure capillary liquid chromatography combined with mass spectrometry (MS). ... and the invention of ultra-high pressure liquid chromatography.[13] His publications in separation methods have been ... "Two-Dimensional High-Performance Liquid Chromatography/Capillary Electrophoresis", J.W. Jorgenson and M.M. Bushey, U.S. Patent ...
Molecular sieve
High-efficient sorption, be used in desiccation, decarburization, desulphurization of gas and liquids and separation of ... Once the beads are full of water, temperature or pressure can be manipulated, allowing the water to be released from the ... Some molecular sieves are used in chromatography, a separation technique that sorts molecules based on their size. Other ... Sweetening (removal of thiols) of aviation fuel and corresponding liquid hydrocarbons 3Å[edit]. *Approximate chemical formula: ...
Buckminsterfullerene
The compound is stable,[24] withstanding high temperatures and high pressures. The exposed surface of the structure can ... Talyzin, A.V. (1997). "Phase Transition C60−C60*4C6H6 in Liquid Benzene". Journal of Physical Chemistry B. 101 (47): 9679-9681 ... These fractions are separated using chromatography.[20] Generally, the fullerenes are dissolved in hydrocarbon or halogenated ... "high density, room temperature, ambient pressure storage of hydrogen". These OBBs are created by binding atoms of a transition ...
Argon
The gas is stored at high pressure.[45]. Argon-39, with a half-life of 269 years, has been used for a number of applications, ... Neutrino experiments include ICARUS and MicroBooNE, both of which use high-purity liquid argon in a time projection chamber for ... Argon may be used as the carrier gas in gas chromatography and in electrospray ionization mass spectrometry; it is the gas of ... Kleppe, Annette K.; Amboage, Mónica; Jephcoat, Andrew P. (2014). "New high-pressure van der Waals compound Kr(H2)4 discovered ...
Lysergic acid diethylamide
Hypertension [high blood pressure], tachycardia [rapid heart-beat], and hyperthermia should be treated symptomatically. ... Liquid solution is either distributed in small vials or, more commonly, sprayed onto or soaked into a distribution medium. ... Non-psychoactive iso-LSD which has formed during the synthesis can be separated by chromatography and can be isomerized to LSD ... Higher doses often cause intense and fundamental distortions of sensory perception such as synesthesia, the experience of ...
Ultraviolet
Small high-pressure lamps can have light focused and transmitted to the work area via liquid-filled or fiber-optic light guides ... UVC LEDs are beginning to be used in disinfection[33] and as line sources to replace deuterium lamps in liquid chromatography ... or offset printing require high-intensity light focused via reflectors onto a moving substrate and medium so high-pressure Hg ( ... After 60 minutes, however, the amount of absorbed sunscreen was so high that the amount of ROS was higher in the sunscreen- ...
Mass spectrometry
Covey TR, Lee ED, Henion JD (October 1986). "High-speed liquid chromatography/tandem mass spectrometry for the determination of ... Matz LM, Asbury GR, Hill HH (2002). "Two-dimensional separations with electrospray ionization ambient pressure high-resolution ... Similar to gas chromatography MS (GC-MS), liquid chromatography-mass spectrometry (LC/MS or LC-MS) separates compounds ... The duty cycle of IMS is short relative to liquid chromatography or gas chromatography separations and can thus be coupled to ...
Estonian Americans
... high pressure liquid chromatography, and the formulation of the Rootare-Prenzlow Equation. ... The pressures of industrialization drove numerous Estonian peasants to emigrate to the United States continuing until the ...
Cannabis strain
... plants by high performance liquid chromatography-mass spectrometry". Analytical and Bioanalytical Chemistry. 406 (29): 7549- ... competition puts pressure on breeders to create increasingly attractive varieties to maintain market share. Breeders give their ... There are five chemotaxonomic types of cannabis: one with high levels of THC, one which is more fibrous and has higher levels ... It does not induce the psychoactive "high" typically associated with recreational marijuana strains that are high in THC.[34] ...
Membrane technology
... the liquid to be filtered flows along the front of the membrane and is separated by the pressure difference between the front ... With high selectivity, isotopes can be enriched (uranium enrichment) in nuclear engineering or industrial gases like nitrogen ... another is measurement of the cut-off by gel permeation chromatography. These methods are used mainly to measure membranes for ... To determine the pore diameter, physical methods such as porosimetry (mercury, liquid-liquid porosimetry and Bubble Point Test ...
Health effects of tobacco
It also causes peripheral arterial disease and high blood pressure. The effects depend on the number of years that a person ... "Determination of aliphatic and aromatic aldehydes in cigarette smoke by gas chromatography with flame photometric detection". ... Smoking also increases blood pressure and weakens blood vessels.[72] Renal[edit]. In addition to increasing the risk of kidney ... A very large percentage of schizophrenics smoke tobacco as a form of self-medication.[125][126][127][128] The high rate of ...
Floral scent
Liquid chromatography may be used for liquid extractions of floral tissue. Detection and identificationEdit. Separation systems ... Floral scent emissions also vary along floral development, with highest emissions at anthesis,[19] i.e. when the flower is ... this phenotypic trait is subject to antagonistic selection pressures (positive selection by pollinators and negative selection ... Gas chromatography (GC) is ideal to separate volatilized VOCs due to their low molecular weight. VOCs are carried by a gas ...
Chromatography - Simple English Wikipedia, the free encyclopedia
High-performance liquid chromatography (HPLC) is also sometimes referred to as high-pressure liquid chromatography. It is ... Thin layer chromatography[change , change source]. Main article: Thin layer chromatography. Thin layer chromatography (TLCC) is ... Column chromatography[change , change source]. Column chromatography separates compounds using many chemical actions between ... Double-way paper chromatography, also called 'two-dimensional chromatography', uses two solvents and rotates the paper 90° in ...
High Pressure Liquid Chromatography (HPLC) - Chemistry | Lycoming College
High Pressure Liquid Chromatography (HPLC). High Pressure Liquid Chromatography (HPLC). ... High Pressure Liquid Chromatography (HPLC) is an important separation technique for compounds of low or no volatility thus it ... The liquid mobile phase is the variable. Both the polarity and pH of the mobile phase can be varied. We possess a Waters 510 ...
High-pressure-liquid-chromatography dictionary definition | high-pressure-liquid-chromatography defined
... of chromatography in which the liquid mobile phase is forced through a column of solid stationary phase under high pressure ... ... of chromatography in which the liquid mobile phase is forced through a column of solid stationary phase under high pressure ... ... high-pressure-liquid-chromatography definition: Noun 1. (chemistry) A form ... high-pressure-liquid-chromatography definition: Noun 1. (chemistry) A form ...
High Pressure Liquid Chromatography-125J-RIA, A Sensitive and Specific Prostaglandin Assay | SpringerLink
High Pressure Liquid Chromatography Resolution Factor Platelet Free Plasma High Pressure Liquid Chromatography Grade High ... Mourits-Andersen T., Jensen R., Dyerberg J. (1985) High Pressure Liquid Chromatography-125J-RIA, A Sensitive and Specific ... High Pressure Liquid Chromatography-125J-RIA, A Sensitive and Specific Prostaglandin Assay. ... Skrinska, V., 1983, High-performance liquid chromatography of prostacyclin, J of Chromatography, 277: 287.CrossRefGoogle ...
High Pressure Liquid Chromatography - Stock Image C009/4186 - Science Photo Library
High pressure liquid chromatography) Biochemist analyses a new compound synthesized by microcide chemists for its interaction ... High pressure liquid chromatography) Biochemist analyses a new compound synthesized by microcide chemists for its interaction ... Keywords: biochemist, biomedical research, chemist, chemistry, female, high pressure liquid chromatography, hplc, ... Licence fees: A licence fee will be charged for any media (low or high resolution) used in your project. ...
High-pressure liquid chromatography (HPLC) is a m... - OpenStudy
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The ... High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The ... High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The ... If an HPLC procedure is running at a pressure of 5.10×10^8 , what is its running pressure in torr? ...
Determination of Glucosamine in Human Plasma by High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization...
Chromatography Research International is a peer-reviewed, Open Access journal that publishes original research and review ... A sensitive, specific, and rapid high-performance liquid chromatography-atmospheric pressure chemical ionization source-tandem ... Determination of Glucosamine in Human Plasma by High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization ... Chromatography Research International. Volume 2011, Article ID 815183, 7 pages. http://dx.doi.org/10.4061/2011/815183. Research ...
Automated preparation of biological samples prior to high pressure liquid chromatography: Part I- The use of dialysis for...
Ion-pair high pressure liquid chromatography techniques for the determination of quaternary ammonium compounds
... Public Deposited ... Separations of Qams using ion-pair liquid chromatography were developed using reverse phase columns and both alkyl sulfonic ... acid and tetramethylammonium salts in the mobile phase as ion-pair liquid chromatography reagents. A continuous post-column ... Special apparatus for post-column solvent extraction of liquid chromatographic eluates was developed as part of this work. ...
https://www.thefreelibrary.com/Rapid+quantification+of+resveratrol+in+mouse+plasma+by+ultra+high...-a0394346487
... general Fluorides High performance liquid chromatography Mass spectrometry Resveratrol ... Rapid quantification of resveratrol in mouse plasma by ultra high pressure liquid chromatography (UPLC) coupled to tandem mass ... Ultra high pressure liquid chromatography Chromatographic separation was achieved by injecting 5 [micro]L of sample into a ... 21) to detect resveratrol in rat plasma by high pressure liquid chromatography (HPLC) has a run time of ,20 min and a LOQ of ...
Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of vitamin E forms in palm-derived...
... accurate and precise normal phase high pressure liquid chromatographic method was developed and validated for the simultaneous ... Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of vitamin E forms in palm-derived ... Fast gradient normal-phase high pressure liquid chromatography for rapid baseline separation of vitamin E forms in palm-derived ... A rapid, accurate and precise normal phase high pressure liquid chromatographic method was developed and validated for the ...
Chromatography, High Pressure Liquid | Profiles RNS
High Pressure Liquid" by people in this website by year, and whether "Chromatography, High Pressure Liquid" was a major or ... Chromatography, High Pressure Liquid*Chromatography, High Pressure Liquid. *Chromatography, Liquid, High Pressure ... Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. ... "Chromatography, High Pressure Liquid" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, ...
Muramic Acid Measurements for Bacterial Investigations in Marine Environments by High-Pressure Liquid Chromatography | Applied...
Advantages of muramic acid measurement by high-pressure liquid chromatography are its high sensitivity and reduction of ... Muramic Acid Measurements for Bacterial Investigations in Marine Environments by High-Pressure Liquid Chromatography. Toru ... Muramic acid, a constituent of procaryotic cell walls, was assayed by high-pressure liquid chromatography in samples from ... Muramic Acid Measurements for Bacterial Investigations in Marine Environments by High-Pressure Liquid Chromatography ...
Advancing Food Analysis with Ultrahigh-Pressure Liquid Chromatography and High-Resolution Mass Spectrometry
... and high-resolution mass spectrometry (HRMS). Jon Wong of the Center for Food Safety and Applied Nutrition at the U.S. Food and ... in food analysis are increasingly interested in taking advantage of methods that harness the power of ultrahigh-pressure liquid ... Advancing Food Analysis with Ultrahigh-Pressure Liquid Chromatography and High-Resolution Mass Spectrometry. ... View MoreLiquid Chromatography (LC/HPLC)Gas Chromatography (GC)Sample PreparationMass SpectrometryPharmaceutical Analysis ...
Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. | Circulation...
SepPak C-18 purification preceded reverse phase high pressure liquid chromatography. High pressure liquid chromatography ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography.. M I Phillips, B ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. ...
Analysis of Poly-β-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and...
Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-β-hydroxybutyrate (PHB) in Rhizobium ... Analysis of Poly-β-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and ... Analysis of Poly-β-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and ... Analysis of Poly-β-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and ...
A novel method for prenylquinone profiling in plant tissues by ultra-high pressure liquid chromatography-mass spectrometry -...
Here we present an original and rapid approach using ultra-high pressure liquid chromatography-atmospheric pressure chemical ... Conventional methods are based on liquid chromatography coupled to ultraviolet and fluorescence detection of a single or ... Prenylquinone levels were measured in leaves of ,i,Arabidopsis,/i, grown under normal and high light intensities. ... Mass spectrometry and chromatography parameters were optimized using pure standards. Sample preparation time was kept to ...
Analysis of sugars in glycoproteins by high-pressure liquid chromatography. - Semantic Scholar
This, together with the use of high-pressure liquid chromatography rather than gas chromatography for separating the sugar ... of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of ... High performance liquid chromatography High pressure liquid chromatography High price liquid chromatography Important ... Analysis of sugars in glycoproteins by high-pressure liquid chromatography.. @article{Jentoft1985AnalysisOS, title={Analysis of ...
MEASUREMENT OF PLASMA CHOLECYSTOKININS (CCKS) USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY (HPLC) AND RADIOIMMUNOASSAY (RIA) -...
MEASUREMENT OF PLASMA CHOLECYSTOKININS (CCKS) USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY (HPLC) AND RADIOIMMUNOASSAY (RIA) - ... MEASUREMENT OF PLASMA CHOLECYSTOKININS (CCKS) USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY (HPLC) AND RADIOIMMUNOASSAY (RIA) - ... MEASUREMENT OF PLASMA CHOLECYSTOKININS (CCKS) USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY (HPLC) AND RADIOIMMUNOASSAY (RIA) - ...
Improved analysis of ladderane lipids in biomass and sediments using high performance liquid chromatography/atmospheric...
A method ... read more combining high-performance liquid chromatography coupled to positive ion atmospheric pressure chemical ... analysis of ladderane lipids in biomass and sediments using high performance liquid chromatography/atmospheric pressure ... analysis of ladderane lipids in biomass and sediments using high performance liquid chromatography/atmospheric pressure ... the ladderane lipids are difficult to analyze by gas chromatography. ...
reagents-chromatography-liquid-high-pressure
Entry Terms : "High-Performance Liquid Chromatography (HPLC) Reagents" , "Chromatography Reagents, Liquid, High Pressure (HPLC ... Liquid, High-Pressure. Definition : Reagents intended for chromatography tests using a liquid phase forced by high pressure to ... High-Pressure Liquid Chromatography) Reagents" , "Homovanillic Acid (HVA) Determination Reagents" , "High-Pressure Liquid ... High-pressure liquid chromatography reagents are frequently used in the clinical laboratory to identify trace quantities of ...
Stereoselective Quantification of Methadone and a d 6-labeled Isotopomer Using High Performance Liquid Chromatography...
Therefore, the authors developed a stereoselective high performance liquid chromatography-atmospheric pressure chemical ... The compounds were quantified in a single assay after liquid-liquid extraction and stereoselective high performance liquid ... Quantification of Methadone and a d6-labeled Isotopomer Using High Performance Liquid Chromatography-Atmospheric Pressure ... chromatograph with atmospheric pressure chemical ionization-mass spectrometry detection. The following ions were monitored: m/z ...
High Pressure Testing / Analytical Chemistry | University of Calgary
HP Liquid Chromatography. Used to separate, identify, and quantify each component in a mixture. Detection is performed via a ... 300 mL to 1 L stirred autoclaves designed to study reactions under sour gas conditions at high pressure and high temperature. ... The in-house built densimeter can be operated up to p= 350 bar and T= 150°C, for measurement of high pressure acid gas fluids ... The High Pressure Multicomponent Adsorption instrument is designed for manometric isotherm measurement at p= 0.1 to 80 bar and ...
Analysis of Underivatized Amphetamines and Related Phenethylamines with High-Performance Liquid Chromatography-Atmospheric...
A novel Fer/FerT targeting compound selectively evokes metabolic stress and necrotic death in malignant cells | Nature...
High pressure liquid chromatography quantification. Quantification of intracellular ATP was carried out using high pressure ... High expression of FER tyrosine kinase predicts poor prognosis in clear cell renal cell carcinoma. Oncol. Lett. 5, 473-478 ( ... liquid chromatography (HPLC) as previously described7.. Measurement of the mitochondrial OXPHOS complex I activity. Activity of ... Interestingly, the high-grade metastatic CC and pancreatic cancer cells were more sensitive to E260 than low grade CC and ...
Chromatography and Mass Spectrometry Conferences | High-Pressure Liquid Chromatography | HPLC-MS | USA | CA | EU
Analytical Chemistry, Separation Sciences, Medicinal Chemistry, Bio Chemistry, Bio Analytical Chemistry, Water Chemistry, Separation Sciences, Green Chemistry, Inorganic Chemistry, Organic Chemistry, Forensic Chemistry, Industrial Chemistry, Gas chromatography, Pharmacology, and Pharmaceutical Sciences.
High Pressure Liquid Chromatography (HPLC)
Fittings and tubing comprise a vital, but often ignored portion of the HPLC system. At first glance, the inexperienced eye sees little or no difference between fittings styles and brands. Although these components are not particularly complicated, a solid understanding of their application and use is essential to avoid certain HPLC problems.. ...
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Bonded-phase chromatographyThin-Layer ChromatoDeterminationLife SciencesChemistryUrineSeparationPurificationDetectionSensitivityColumnsUHPLCAntioxidant propertiesSeparationsTandemMeSHMobile phaseAnalyzerReverse phaseSolventUPLCExtractionBiologicalAssaySignificantlyReproducibilityElutionMethodsPhasePerformanceApparatusQuadrupoleAnalysisMolecularMass SpectrometerQuantificationLipidsWatersMethodSystemSpectrometryAdsorption1000PhasesSerumGeneColumn
Bonded-phase chromatography1
- The functional groups of the stationary phase may be polar relative to those of the mobile phase (i.e., normal bonded-phase chromatography) or, more usually, nonpolar (i.e., reverse bonded-phase chromatography). (ophthalmologymanagement.com)
Thin-Layer Chromato2
- Similarly, in two different thin-layer chromatography systems, all TRH immunoreactivity migrated with that of synthetic TRH. (elsevier.com)
- Spindel, E & Wurtman, RJ 1980, ' TRH immunoreactivity in rat brain regions, spinal cord and pancreas: validation by high-pressure liquid chromatography and thin-layer chromatography ', Brain research , vol. 201, no. 2, pp. 279-288. (elsevier.com)
Determination11
- A sensitive, specific, and rapid high-performance liquid chromatography-atmospheric pressure chemical ionization source-tandem mass spectrometry (HPLC-APCI-MS/MS) method for the determination of glucosamine in human plasma was developed and validated. (hindawi.com)
- Methods: We used reversed-phase ultra high pressure liquid chromatography with tandem mass spectrometry detection for the determination of resveratrol levels in mouse plasma. (thefreelibrary.com)
- Metodos: Utilizamos cromatografia liquida con presion ultra alta acoplada a espectrometria de masa en serie, para la determination de los niveles de resveratrol en plasma de raton. (thefreelibrary.com)
- A rapid, accurate and precise normal phase high pressure liquid chromatographic method was developed and validated for the simultaneous determination of nine vitamin E forms comprised of α-tocomonoenol, α-, β-, γ-, and δ-tocopherols and α-, β-, γ-, and δ-tocotrienols in tocotrienol rich fractions (TRFs) derived from palm oil. (rsc.org)
- Allows for the determination of sulfur viscosity as a function of shear rate and/or temperature under high pressures. (ucalgary.ca)
- A method for the determination of cocaine (COC), benzoylecgonine (BE), ecgonine methyl ester (EME), and ecgonine (ECG) in plasma by liquid chromatography-mass spectrometry (LC-MS-MS) was developed. (oup.com)
- A new and simple method for the determination of rat liver microsomal uridine diphosphate glucuronosyltransferase activity toward 4-nitrophenol, phenolphthalein, and testosterone has been developed with the use of high-pressure liquid chromatography (hplc). (elsevier.com)
- A Comparison of Three Different High Pressure Liquid Chromatography Systems for the Determination of Aldehydes and Ketones in Diesel Exhaust," Journal of Chromatographic Science , Oxford University Press, Jan 1982. (mst.edu)
- Hordijk, CA & Cappenberg, TE 1983, ' Quantitative High-Pressure Liquid Chromatography-Fluorescence Determination of Some Important Lower Fatty Acids in Lake Sediments ', Applied and Environmental Microbiology , vol. 46, no. 2, pp. 361-369. (knaw.nl)
- In the present study, we developed and validated a method that uses liquid extraction coupled with HPLC-MS/MS determination to measure TCC in feces. (elsevier.com)
- An accurate, simple, and sensitive method for the direct determination of proteins by nonspecies specific isotope dilution and external calibration high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) is described. (gc.ca)
Life Sciences1
- AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for Molecular Biology, Life Sciences, Proteomics, Clinical and Histology areas of research. (ophthalmologymanagement.com)
Chemistry5
- High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. (openstudy.com)
- D. C. Turnell and J. D. H. Cooper, "Automated preparation of biological samples prior to high pressure liquid chromatography: Part I- The use of dialysis for deproteinizing serum for amino-acid analysis," Journal of Automatic Chemistry , vol. 7, no. 4, pp. 177-180, 1985. (hindawi.com)
- Turbulent flow liquid chromatography was developed in the late 1990s, and combines 'size exclusion' and traditional stationary phase column chemistry to separate macromolecules, such as proteins, from smaller molecules and analytes of interest in biological fluids. (thermofisher.com)
- High pressure liquid chromatography (HPLC) is typically used to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying or purifying the individual components of the mixture. (gre.ac.uk)
- High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. (wikipedia.org)
Urine4
- Lu H, Yu J, Wu L, Xing J, Wang J, Huang P, Zhang J, Xiao H, Gao R. Optimized ultra performance liquid chromatography tandem high resolution mass spectrometry method for the quantification of paraquat in plasma and urine. (umassmed.edu)
- Lu H, Yu J, Wang J, Wu L, Xiao H, Gao R. Simultaneous quantification of neuroactive dopamine serotonin and kynurenine pathway metabolites in gender-specific youth urine by ultra performance liquid chromatography tandem high resolution mass spectrometry. (umassmed.edu)
- Baclofen was identified in urine by gas chromatography/mass spectrometry. (mendeley.com)
- After derivatization with trinitrobenzene sulfonic acid, baclofen was quantitated in serum and urine by high-performance liquid chromatography. (mendeley.com)
Separation7
- High Pressure Liquid Chromatography (HPLC) is an important separation technique for compounds of low or no volatility thus it makes a nice complimentary procedure to GC. (lycoming.edu)
- The Bruker Maxis 4G ETD Ion Mobility (incl UPLC) combines high resolution/accurate mass with separation based on molecular shape (by Ion Mobility). (enablingtechnologies.eu)
- The separation was achieved by high pressure liquid chromatography on a Vydac pellicular anion exchange column using a linear gradient of 0.5 to 1.0 m acetic acid as the eluting solvent. (naver.com)
- To ensure high separation efficiency, low gradient response times and superior mixing performance we offer perfectly matched HPLC and UHPLC system components. (thermofisher.com)
- Skalnikova, H., Halada, P., Dzubak, P., Hajduch, M. and Kovarova, H. (2005) Protein fingerprints of anti-cancer effects of cyclin-dependent kinase inhibition: 320 identification of candidate biomarkers using 2-d liquid phase separation coupled to mass spectrometry. (openaire.eu)
- HPLC relies on pumps to pass a pressurized liquid and a sample mixture through a column filled with adsorbent, leading to the separation of the sample components. (wikipedia.org)
- The use of smaller particle size packing materials requires the use of higher operational pressure ("backpressure") and typically improves chromatographic resolution (the degree of peak separation between consecutive analytes emerging from the column). (wikipedia.org)
Purification2
- To answer directly the question of whether angiotensin II exists in the brain, independent of blood-borne angiotensin, and to quantify the amounts in different parts of the central nervous system, a sensitive radioimmunoassay was used to measure extracts of male adult rat brain hypothalamus and cortex after purification with high pressure liquid chromatography with a high recovery. (ahajournals.org)
- SepPak C-18 purification preceded reverse phase high pressure liquid chromatography. (ahajournals.org)
Detection5
- Zheng J, Bi J, Johnson D, Sun Y, Song M, Qiu P, Dong P, Decker E, Xiao H. Analysis of 10 metabolites of polymethoxyflavones with high sensitivity by electrochemical detection in high-performance liquid chromatography. (umassmed.edu)
- Conventional methods are based on liquid chromatography coupled to ultraviolet and fluorescence detection of a single or limited number of prenylquinones at a time. (rero.ch)
- The compounds were quantified in a single assay after liquid-liquid extraction and stereoselective high performance liquid chromatograph with atmospheric pressure chemical ionization-mass spectrometry detection. (ovid.com)
- high pressure liquid chromatography ( HPLC ) is one of the best methods for screening and detection of various hemoglobinopathies but it has intrinsic interpretive problems. (bvsalud.org)
- Sensitivity of the method was slightly less than that of our GC-MS method, but significantly higher compared to the HPLC methods using diode-array or UV detection. (nih.gov)
Sensitivity6
- Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (umassmed.edu)
- Advantages of muramic acid measurement by high-pressure liquid chromatography are its high sensitivity and reduction of preparation steps, allowing a short time analysis. (asm.org)
- For high-resolution and high-sensitivity separations that are ideally suited for use with mass spectrometry and designed for laboratories with less throughput or where sample preparation is done as a separate step in their analytical workflow. (thermofisher.com)
- To evaluate the sensitivity and specificity of denaturing high pressure liquid chromatography (DHPLC) screening for unknown protein C gene (PROC) mutations, we studied 31 PROC-deficient patients. (elsevier.com)
- Accurate mass measurements to determine elemental composition, high sensitivity MS and MS/MS measurements. (gre.ac.uk)
- Ultra high pressure liquid chromatography (UHPLC) typically using 1.7 micron column particle size provides significantly more resolution (information) while reducing run times, and improves sensitivity for the analyses of many compound types. (gre.ac.uk)
Columns2
- Separations of Qams using ion-pair liquid chromatography were developed using reverse phase columns and both alkyl sulfonic acid and tetramethylammonium salts in the mobile phase as ion-pair liquid chromatography reagents. (oregonstate.edu)
- Activity in the extracts was separated by high pressure liquid chromatography, using reverse phase and molecular sieve columns. (edu.au)
UHPLC6
- Specialists in food analysis are increasingly interested in taking advantage of methods that harness the power of ultrahigh-pressure liquid chromatography (UHPLC) and high-resolution mass spectrometry (HRMS). (chromatographyonline.com)
- Q. You collaborated in developing a method for analyzing multiple mycotoxins in finished grain and nut products using ultrahighâ pressure liquid chromatography and highâ resolution mass spectrometry (UHPLC-HRMS) (1). (chromatographyonline.com)
- The results showed that the same product ions are formed in both LC-MS/MS and UHPLC-HRMS despite the different MS platforms, but the high-accuracy masses in HRMS provided better details to speculate about how the mycotoxin precursor could fragment and form into product ions. (chromatographyonline.com)
- We were comparing and matching the mass-to-charge ratio (m/z) results from product ions obtained from our UHPLC -high-resolution data-dependent MS/MS experiments of ergocornine and its products with results obtained from LC-MS/MS (using a tripleâ quadrupole mass analyzer), provided from a publication from another research group (3). (chromatographyonline.com)
- Our UHPLC-high-resolution dataâ dependent MS/MS results agreed with most of the results except for m/z 208.1. (chromatographyonline.com)
- Here we present an original and rapid approach using ultra-high pressure liquid chromatography-atmospheric pressure chemical ionization-quadrupole time-of-flight mass spectrometry (UHPLC-APCI- QTOFMS) for the simultaneous profiling of eleven prenylquinones in plant tissues, including a-tocopherol, phylloquinone, plastochromanol-8 and plastoquinone-9. (rero.ch)
Antioxidant properties2
- In the current study, an innovative combination of high hydrostatic pressure and enzymatic hydrolysis (HHP-EH) was used to increase the yield of short bioactive peptides, and evaluate the anti-inflammatory and antioxidant properties of the BLGH produced by the HHP-EH process. (mdpi.com)
- The BLGH produced by alcalase (Alc) (BLG-Alc) showed significantly higher antioxidant properties among the six enzymes examined in this study. (mdpi.com)
Separations1
- Park, K.H., Lipuma, J.J. and Lubman, D.M. (2007) Comparative proteomic analysis of b. cenocepacia using two-dimensional liquid separations coupled with 340 mass spectrometry. (openaire.eu)
Tandem3
- Rapid quantification of resveratrol in mouse plasma by ultra high pressure liquid chromatography (UPLC) coupled to tandem mass spectrometry. (thefreelibrary.com)
- combining high-performance liquid chromatography coupled to positive ion atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS) was developed for the analysis of the most abundant ladderane lipids, occurring as fatty acids and ether-bound to glycerol. (uu.nl)
- Seven PFAS were detected in almost all participant sera by ultra-high performance liquid chromatograph/tandem mass spectrometry. (nih.gov)
MeSH1
- Chromatography, High Pressure Liquid" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Mobile phase3
- The liquid mobile phase is the variable. (lycoming.edu)
- The extracts were examined with high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS). The drugs were separated on ODS column in acetonitrile/50mM ammonium formate buffer (pH 3.0) (25:75) as a mobile phase. (oup.com)
- HPLC is distinguished from traditional ("low pressure") liquid chromatography because operational pressures are significantly higher (50-350 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the column. (wikipedia.org)
Analyzer1
- Surface characterization analyzer that is capable of performing physisorption and chemisorption analyses with high accuracy and resolution. (ucalgary.ca)
Reverse phase2
- A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. (semanticscholar.org)
- These derivatives had different retention times than the original material on reverse phase chromatography, but there was still correspondence of the peaks from oxidized extracts and oxidized authentic somatostatin. (edu.au)
Solvent4
- Special apparatus for post-column solvent extraction of liquid chromatographic eluates was developed as part of this work. (oregonstate.edu)
- 1. A process for annealing amorphous atorvastatin at elevated temperature comprising heating at ambient pressure amorphous atorvastatin in an essentially solvent-free system to a temperature of between approximately 50° C. to approximately 140° C. (freepatentsonline.com)
- 5. The top of the jar must be replaced and the apparatus is left in the fume cupboard until the solvent has run up the chromatography paper. (markedbyteachers.com)
- It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. (wikipedia.org)
UPLC1
- As a first step to address these questions, we applied ultra-high pressure liquid chromatography coupled with quadrupole-orbitrap ultra-high resolution mass spectrometry (UPLC-Quadrupole-Orbitrap-UHRMS) to monitor peptides dynamic changes during the withering process. (bioinfor.com)
Extraction2
- Sample preparation time was kept to minimum and different extraction solvents were evaluated for yield, ability to maintain the redox state of prenylquinones, and compatibility with chromatography. (rero.ch)
- We offer access to a range of equipment, including a variety of complementary chromatography techniques, ionisation sources and extraction methods. (gre.ac.uk)
Biological2
- High pressure liquid chromatography) Biochemist analyses a new compound synthesized by microcide chemists for its interaction with biological macromolecules using HPLC. (sciencephoto.com)
- Invasive species can quickly transform biological communities due to their high abundance and strong impacts on native species, in part because they can be released from the ecological forces that limit native populations. (mendeley.com)
Assay2
- Therefore, the authors developed a stereoselective high performance liquid chromatography-atmospheric pressure chemical ionization mass-spectrometry assay for the quantification of the enantiomers of methadone and a d6-labeled isotopomer. (ovid.com)
- Antibacterial assay revels Lawsonia inermis showed highest zone of inhibition on Proteus sps. (ijper.org)
Significantly1
- The lower Ad-SoS SDS reflects reduced bone status, and the reduction was significantly more marked in those patients whose HbA 1c T was higher than 7.0% when compared with those whose HbA 1c T was lower. (springer.com)
Reproducibility1
- High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode. (openaire.eu)
Elution1
- We extracted protein isoforms from polyacrylamide gels by passive elution using SDS, followed by nanoscale hydrophilic phase chromatography for SDS removal. (mcponline.org)
Methods1
- Conventional methods are based on liquid chromatography coupled to ultraviolet and fluorescence. (rero.ch)
Phase4
- Reagents intended for chromatography tests using a liquid phase forced by high pressure to carry the sample through the stationary material. (ophthalmologymanagement.com)
- A normal-phase Bondapak high performance liquid chromatographic (HPLC) system was compared to two different reversed-phase Bondapak C//1//8 systems for separating and quantifying aldehydes and ketones. (mst.edu)
- Thyrotropin-releasing hormone (TRH) immunoreactivity in rat tissue was assayed using a new reversed-phase, ion-pair, high-pressure liquid chromatography (HPLC) method for separating TRH from its analogs. (elsevier.com)
- Some HPLC techniques use water-free mobile phases (see normal-phase chromatography below). (wikipedia.org)
Performance6
- The extracts were examined with high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS). The drugs were separated on ODS column in acetonitrile/50mM ammonium formate buffer (pH 3.0) (25:75) as. (oup.com)
- ES Industries offers the HPLC chromatographer an important source for high performance bonded phases and packing materials. (ophthalmologymanagement.com)
- For clinical translational research, most laboratories seek high performance systems to meet their demanding needs in addition to support the connection to their mass spectrometer. (thermofisher.com)
- Selection by the Scholarship Committee will be based upon the applicant's academic performance in high school, leadership, extracurricular involvement and community service. (wpi.edu)
- We developed and validated a denaturing high performance liquid chromatograph [DHPLC] method for the molecular analysis of SLC6A8 and GAMT genes involve in creatine biosynthesis and transport as a possible source of human male infertility by analyzing DNA from 64, clinically confirmed, infertile men . (bvsalud.org)
- Improving column performance remains paramount to advancing liquid chromatography (LC) technology. (unc.edu)
Apparatus3
- A method for making a liquid-chromatography apparatus includes inserting two inner conduits into an intermediate tube, inserting the intermediate tube into an outer tube, forming a proximal seal between the intermediate tube and at least one of the inner conduits, and forming a distal seal between the intermediate tube and at least one of the inner conduits. (patentsencyclopedia.com)
- 24. The apparatus of claim 22, further comprising a fluidic interface disposed at the interface between the liner tube and the optical conduit to provide a fluid passageway in communication with the liquid in the liner tube. (patentsencyclopedia.com)
- [0002] The invention generally relates to chromatography apparatus that operate at high pressure. (patentsencyclopedia.com)
Quadrupole1
- Achieve up to four times throughput on your single triple quadrupole or high resolution mass spectrometer using the multi-channel turbulent flow liquid chromatography system. (thermofisher.com)
Analysis7
- Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis. (asm.org)
- To understand the mechanisms involved in the response of plants to changing conditions such as high light intensity, the comprehensive analysis of these apolar lipids is an essential but challenging step. (rero.ch)
- Analysis of sugars in glycoproteins by high-pressure liquid chromatography. (semanticscholar.org)
- Direct analysis in real time (DART) is an atmospheric pressure ion source that operates by exposing the sample to a gas stream (typically helium or nitrogen) that contains long-lived electronically or excited neutral atoms, vibronically excited molecules. (gre.ac.uk)
- It is also useful for analysis of high mass species that form multiply charged ions with ESI (such as proteins, peptides) due to the post-acquisition de-convolution capability of the operating software. (gre.ac.uk)
- Sumudu D L L6B Monday, October 21, 2002 Analysis of amino acids by paper chromatography Introduction- Proteins may be thought of natural polymers of amino acids, as the composition of proteins is of amino acids. (markedbyteachers.com)
- The technique known as paper chromatography is used to separate amino acids for analysis. (markedbyteachers.com)
Molecular3
- Molecular sieving using Sephadex G-25 with acetic acid revealed a distinct peak in the 1000 MW range and a smaller, higher molecular weight peak which needs further investigation. (ahajournals.org)
- To our knowledge, these are the highest numbers of molecular compositions of O2NAs that have been profiled thus far in environmental samples. (nih.gov)
- Is high pressure liquid chromatography an effective screening tool for characterization of molecular defects in hemoglobinopathies. (bvsalud.org)
Mass Spectrometer2
- With TurboFlow™ Technology, dramatically increase the return on your capital investment in mass spectrometer technology by improving data quality, lowering per-sample costs and increasing sample throughput using automated online sample preparation and multichannel liquid chromatography multiplexing. (thermofisher.com)
- Gas chromatography mass spectrometry (GC-MS) is an instrumental technique, comprising a gas chromatograph (GC) coupled to a mass spectrometer (MS), by which complex mixtures of chemicals may be separated, identified and quantified. (gre.ac.uk)
Quantification1
- In addition to precise absolute quantification of 5 prenyllipids for which standards were available, relative quantification of 6 other related compounds was possible thanks to the high identification power of QTOFMS. (rero.ch)
Lipids1
- Due to the thermal lability of the strained cyclobutane moieties, the ladderane lipids are difficult to analyze by gas chromatography. (uu.nl)
Waters1
- Waters Corporation has been in the business of making innovative analytical instruments that assist scientists in reaching their scientific goals, increase productivity, and earn laboratory-based organizations a higher return on their investments in research, development, and quality control. (ophthalmologymanagement.com)
Method3
- Method: BLG was enzymatically hydrolyzed by different proteases at an enzyme-to-substrate ratio of 1:100 under HHP (100 MPa) and compared with hydrolysates obtained under atmospheric pressure (AP-EH at 0.1 MPa). (mdpi.com)
- 7. The culture method as claimed in claim 4, which is a liquid culture method. (freepatentsonline.com)
- Unfortunately, the raw packing material contained a significant number of small mesoporous fines that limited the theoretical benefits of these particles, so a hydrodynamic chromatography (HDC) method was developed to remove the fines and reduce the particle size distribution (PSD). (unc.edu)
System3
- ReactIR™ iC10 FTIR based in-situ system to study of the microscopic behavior of adsorbed molecules at pressures up to 100 bar and temperatures up to 300°C. (ucalgary.ca)
- The creatine / phosphocreatine system is essential for cellular phosphate coupled energy storage and production , particularly in tissues subject to high metabolic demands. (bvsalud.org)
- Automated two-dimensional liquid chromatography using the PF2D system from Beckman Coulter provides a fractionation platform well suited for differential proteomic studies. (openaire.eu)
Spectrometry5
- RESULTS AND DISCUSSION: Mass spectrometry and chromatography parameters were optimized using pure standards. (rero.ch)
- Instrument that combines the features of gas-chromatography and mass spectrometry to identify different substances within a sample. (ucalgary.ca)
- Urinary levels of 2-thiothiazolidine-4-carboxylic acid (a marker of carbon disulfide and vulcanization fumes) were assessed by liquid chromatography coupled with mass spectrometry. (ilo.org)
- High-intensity, spiked noise is reduced in chromatography-mass spectrometry data by applying a nonlinear filter such as a moving median filter to the data. (google.com)
- Standard linear filters are not effective for reducing noise in liquid chromatography-mass spectrometry (LC-MS) data because they assume a normal distribution of noise. (google.com)
Adsorption3
- The High Pressure Multicomponent Adsorption instrument is designed for manometric isotherm measurement at p= 0.1 to 80 bar and T= -20 to 120 °C. (ucalgary.ca)
- Zero-Length Chromatography (ZLC) is an analytical instrument for studying the equilibrium and kinetic behavior gas adsorption in porous materials. (ucalgary.ca)
- Chromatography can be described as a mass transfer process involving adsorption. (wikipedia.org)
10001
- High pressure differential scanning calorimeter for measurements of various fluids at a wide range of temperatures, and at pressures up to 1000 bars. (ucalgary.ca)
Phases1
- High pressure liquid chromatography (HPLC) stationary phases based on carbon-coated silica have better mechanical strength and retentivity than pure carbon phases which makes them ideal for two-dimensional liquid chromatography. (umn.edu)
Serum1
- High serum serotonin in sudden infant death syndrome. (umassmed.edu)
Gene1
- The absence of functionally important polymorphisms in the COX-2 gene may suggest that there has been selective pressure against those single nucleotide polymorphisms because of the critical role of this enzyme in maintenance of homeostasis. (aspetjournals.org)
Column3
- Muramic acid, a constituent of procaryotic cell walls, was assayed by high-pressure liquid chromatography in samples from several marine environments (water column, surface microlayer, and sediment) and a bacterial culture. (asm.org)
- Cation Chromatography separates and quantifies positive ions in aqueous samples, based on their affinity with the ion exchanger column. (ucalgary.ca)
- High-pressure packed-column liquid chromatography systems in which the packing of the column is made of microparticulate silica gel. (ophthalmologymanagement.com)