Gels
Chromatography, High Pressure Liquid
Chromatography, Affinity
Chromatography, Gel
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography, Ion Exchange
Chromatography, Thin Layer
Chromatography, DEAE-Cellulose
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Electrophoresis, Gel, Two-Dimensional
Chromatography, Agarose
Electrophoresis, Polyacrylamide Gel
Amino Acid Sequence
Mass Spectrometry
Gas Chromatography-Mass Spectrometry
Chromatography, Reverse-Phase
Electrophoresis, Gel, Pulsed-Field
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Hydrogen-Ion Concentration
Chromatography, Paper
Electrophoresis, Agar Gel
Isoelectric Focusing
Substrate Specificity
Amino Acids
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Tandem Mass Spectrometry
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Base Sequence
Spectrophotometry, Ultraviolet
Chemistry
Chemical Phenomena
Cattle
Rabbits
Chemical Fractionation
Peptide Fragments
Spectrometry, Mass, Electrospray Ionization
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
Temperature
Protein Binding
Trypsin
Liver
Carbohydrates
Electrophoresis
Countercurrent Distribution
Chromatography, Micellar Electrokinetic Capillary
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Magnetic Resonance Spectroscopy
Cloning, Molecular
Carbohydrate Sequence
Hydroxyapatites
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Binding Sites
Isoelectric Point
Oligosaccharides
Centrifugation, Density Gradient
Chemical Precipitation
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Macromolecular Substances
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Solubility
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Immunodiffusion
Ultracentrifugation
Isoenzymes
Microchemistry
Reference Standards
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Calibration
Immunoelectrophoresis
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Lectins
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Carrier Proteins
Fatty Acids
Sequence Homology, Amino Acid
Glycoproteins
Rats, Inbred Strains
Stereoisomerism
Cells, Cultured
Brain Chemistry
Physicochemical Phenomena
Cell Membrane
Detergents
Glycopeptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Plant Extracts
Enzyme Stability
Chemistry, Physical
Spectrophotometry
Durapatite
Blotting, Western
Blood Proteins
Chemistry Techniques, Analytical
Anion Exchange Resins
Phospholipids
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Molecular Structure
Solvents
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Endopeptidases
Chickens
Methanol
Immune Sera
Limit of Detection
Sensitivity and Specificity
Peptide Hydrolases
Cytosol
Solid Phase Extraction
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Binding, Competitive
Chymotrypsin
Mutation
Isomerism
Glycolipids
Plants
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Cross Reactions
Spectrometry, Mass, Fast Atom Bombardment
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Cross-Linking Reagents
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Plasmids
Circular Dichroism
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Erythrocytes
Membrane Proteins
Vaginal Creams, Foams, and Jellies
Medicated dosage forms for topical application in the vagina. A cream is a semisolid emulsion containing suspended or dissolved medication; a foam is a dispersion of a gas in a medicated liquid resulting in a light, frothy mass; a jelly is a colloidal semisolid mass of a water soluble medicated material, usually translucent.
Flame Ionization
Protease Inhibitors
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
Affinity Labels
Glycosides
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Edetic Acid
Cations, Divalent
Structure-Activity Relationship
Immunoblotting
Ultrafiltration
The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).
Biotransformation
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Collagen
Monosaccharides
Mannose
Denaturing Gradient Gel Electrophoresis
Isotope Labeling
Antibodies
Chromatography, Supercritical Fluid
Immunochemistry
Cell Fractionation
Methylation
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Microsomes, Liver
Streptomyces
Galactose
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Electrophoresis, Starch Gel
Fermentation
Plant Lectins
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Tissue Distribution
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Silicon Dioxide
Carbon Radioisotopes
Immunoassay
Sequence Analysis
Glycosylation
Cyanogen Bromide
Phosphorylation
Enzyme Activation
Sialic Acids
Immunoelectrophoresis, Two-Dimensional
Plants, Medicinal
Polyethylene Glycols
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Heparin
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Glycosaminoglycans
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Adsorption
Recombinant Fusion Proteins
Kidney
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Electrophoresis, Disc
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. (1/13503)
1. VLD (very-low-density), LD (low-density) and HD (high-density) lipoproteins were isolated from the serum of trout (Salmo gairdneri Richardson). 2. Each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behaviour and appearance in the electron microscope. Trout LD lipoprotein, however, was of greater density than human LD lipoprotein. 3. The trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, except for their high contents of long-chain unsaturated fatty acids. 4. HD and LD lipoproteins were immunologically non-identical, whereas LD lipoproteins possessed antigenic determinants in common with VLD lipoproteins. 5. VLD and HD lipoproteins each contained at least seven different apoproteins, whereas LD liprotein was composed largely of a single apoprotein which resembled human apolipoprotein B. 6. At least one, and possibly three, apoprotein of trout HD lipoprotein showed features which resemble human apoprotein A-1.7. The broad similarity between the trout and human lipoprotein systems suggests that both arose from common ancestral genes early in evolutionary history. (+info)A protein-glucan intermediate during paramylon synthesis. (2/13503)
A sodium deoxycholate extract containing glucosyltransferase activity was obtained from a particulate preparation from Euglena gracilis. It transferred glucose from UDP-[14C]glucose into material that was precipitated by trichloroacetic acid. This material released beta-(1 leads to 3)-glucan oligosaccharides into solution on incubation with weak acid, weak alkali and beta-(1 leads to 3)-glucosidase. The products of the incubation of the deoxycholate extract with UDP-[14C]glucose were analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Radioactive bands were obtained that had the properties of beta-(1 leads to 3)-glucan covalently linked to protein by a bond labile to weak acid. High-molecular-weight material containing a beta-(1 leads to 3)-glucan was also shown to be present by gel filtration. The bond linking glucan to aglycone is possibly a pyrophosphate linkage. It is proposed that in Euglena gracilis beta-(1 leads to 3)-glucan (paramylon) is synthesized on a protein primer. (+info)Axin prevents Wnt-3a-induced accumulation of beta-catenin. (3/13503)
When Axin, a negative regulator of the Wnt signaling pathway, was expressed in COS cells, it coeluted with glycogen synthase kinase-3beta (GSK-3beta), beta-catenin, and adenomatous polyposis coli protein (APC) in a high molecular weight fraction on gel filtration column chromatography. In this fraction, GSK-3beta, beta-catenin, and APC were co-precipitated with Axin. Although beta-catenin was detected in the high molecular weight fraction in L cells on gel filtration column chromatography, addition of conditioned medium expressing Wnt-3a to the cells increased beta-catenin in the low molecular weight fraction. However, Wnt-3a-dependent accumulation of beta-catenin was greatly inhibited in L cells stably expressing Axin. Axin also suppressed Wnt-3a-dependent activation of Tcf-4 which binds to beta-catenin and acts as a transcription factor. These results suggest that Axin forms a complex with GSK-3beta, beta-catenin, and APC, resulting in the stimulation of the degradation of beta-catenin and that Wnt-3a induces the dissociation of beta-catenin from the Axin complex and accumulates beta-catenin. (+info)Isolation and complete covalent structure of liver microsomal paraoxonase. (4/13503)
Paraoxonase (PON1) is a serum esterase exclusively associated with high-density lipoproteins; it might confer protection against coronary artery disease by destroying pro-inflammatory oxidized lipids in oxidized low-density lipoproteins. Here I show that rabbit liver microsomes contain a PON analogue (MsPON) and report the isolation and complete covalent structure of MsPON. In detergent-solubilized microsomes, MsPON co-purifies with the microsomal triacylglycerol transfer protein (MTP) complex. MsPON was separated from the complex and purified to homogeneity under non-denaturing conditions. Automated sequence analysis of intact MsPON and peptides obtained from enzymic and chemical cleavages led to the elucidation of the complete covalent structure of MsPON. The protein is a single polypeptide consisting of 350 residues. The sequence of rabbit liver microsomal MsPON is 60% identical with that of rabbit serum PON1, and 84% identical with the sequence predicted by a human cDNA of unknown function, designated PON3. MsPON has a hydrophobic segment at the N-terminus that might serve to anchor the protein to the microsomal membrane or to the MTP complex. Unlike in the serum enzyme, two potential N-glycan acceptor sites in MsPON are not glycosylated. An absence of N-glycans was also indicated in the rabbit liver MTP. MsPON has a single free cysteine residue at position 38 and a disulphide bond between Cys-279 and Cys-348. The microsomal enzyme lacks three residues at the N-terminus that are present in the serum protein. MsPON lacks four residues at the C-terminus that are present in the rabbit serum protein but absent from human serum PON1. On the basis of the observation that MsPON displays a high degree of similarity with serum PON1, it is proposed that MsPON might have a function related to that of PON1 in serum high-density lipoprotein complexes. (+info)Simultaneous antisense inhibition of two starch-synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin. (5/13503)
A chimaeric antisense construct was used to reduce the activities of the two major starch-synthase isoforms in potato tubers simultaneously. A range of reductions in total starch-synthase activities were found in the resulting transgenic plants, up to a maximum of 90% inhibition. The reduction in starch-synthase activity had a profound effect on the starch granules, which became extremely distorted in appearance compared with the control lines. Analysis of the starch indicated that the amounts produced in the tubers, and the amylose content of the starch, were not affected by the reduction in activity. In order to understand why the starch granules were distorted, amylopectin was isolated and the constituent chain lengths analysed. This indicated that the amylopectin was very different to that of the control. It contained more chains of fewer than 15 glucose units in length, and fewer of between 15 and 80 glucose units. In addition, the amylopectin contained more very long chains. Amylopectin from plants repressed in just one of the activities of the two starch-synthase isoforms, which we have reported upon previously, were also analysed. Using a technique different to that used previously we show that both isoforms also affect the amylopectin, but in a way that is different to when both isoforms are repressed together. (+info)Purification and characterization of an alpha-galactosyltransferase from Trypanosoma brucei. (6/13503)
A membrane-associated galactosyltransferase from Trypanosoma brucei was purified 34000-fold by affinity chromatography on UDP-hexanolamine-Sepharosetrade mark. Using SDS/PAGE under reducing conditions, the isolated enzyme ran as a relatively broad band with apparent molecular masses of 53 kDa and 52 kDa, indicative of glycosylation and the existence of two isoforms. N-Glycosylation of the enzyme was subsequently confirmed using Western blotting and either specific binding of concanavalin A or peptide-N4-(N-acetylglucosaminyl)asparagine amidase digestion. The de-N-glycosylated enzyme ran with apparent molecular masses of 51 kDa and 50 kDa, indicative of a single N-glycosylation site. The galactosyltransferase exhibited a pH optimum at 7.2 and had a pronounced requirement for Mn2+ ions (KM=2.5 mM) for its action. The transferase activity was independent of the concentration of Triton X-100. The enzyme was capable of transferring galactose from UDP-galactose to a variety of galactose-based acceptors in alpha-glycosidic linkages. The apparent KM values for UDP-galactose and for the preferred acceptor substrate N-acetyl-lactosamine are 46 microM and 4.5 mM respectively. From these results we would like to suggest that the galactosyltransferase functions in the processing of terminal N-acetyl-lactosamine structures of trypanosomal glycoproteins. (+info)Biophysical characterization of the structure of the amino-terminal region of gp41 of HIV-1. Implications on viral fusion mechanism. (7/13503)
A peptide of 51 amino acids corresponding to the NH2-terminal region (5-55) of the glycoprotein gp41 of human immunodeficiency virus type 1 was synthesized to study its conformation and assembly. Nuclear magnetic resonance experiments indicated the sequence NH2-terminal to the leucine zipper-like domain of gp41 was induced into helix in the micellar solution, in agreement with circular dichroism data. Light scattering experiment showed that the peptide molecules self-assembled in water into trimeric structure on average. That the peptide molecules oligomerize in aqueous solution was supported by gel filtration and diffusion coefficient experiments. Molecular dynamics simulation based on the NMR data revealed a flexible region adjacent to the hydrophobic NH2 terminus of gp41. The biological significance of the present findings on the conformational flexibility and the propensity of oligomerization of the peptide may be envisioned by a proposed model for the interaction of gp41 with membranes during fusion process. (+info)Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. (8/13503)
The catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) has greater than 65% amino acid identity to the catalytic subunit of protein phosphatase 2A (PP2AC). Despite this high homology, PP4 does not appear to associate with known PP2A regulatory subunits. As a first step toward characterization of PP4 holoenzymes and identification of putative PP4 regulatory subunits, PP4 was purified from bovine testis soluble extracts. PP4 existed in two complexes of approximately 270-300 and 400-450 kDa as determined by gel filtration chromatography. The smaller PP4 complex was purified by sequential phenyl-Sepharose, Source 15Q, DEAE2, and Superdex 200 gel filtration chromatographies. The final product contained two major proteins: the PP4 catalytic subunit plus a protein that migrated as a doublet of 120-125 kDa on SDS-polyacrylamide gel electrophoresis. The associated protein, termed PP4R1, and PP4C also bound to microcystin-Sepharose. Mass spectrometry analysis of the purified complex revealed two major peaks, at 35 (PP4C) and 105 kDa (PP4R1). Amino acid sequence information of several peptides derived from the 105 kDa protein was utilized to isolate a human cDNA clone. Analysis of the predicted amino acid sequence revealed 13 nonidentical repeats similar to repeats found in the A subunit of PP2A (PP2AA). The PP4R1 cDNA clone engineered with an N-terminal Myc tag was expressed in COS M6 cells and PP4C co-immunoprecipitated with Myc-tagged PP4R1. These data indicate that one form of PP4 is similar to the core complex of PP2A in that it consists of a catalytic subunit and a "PP2AA-like" structural subunit. (+info)
Cathepsin D from pig myometrium. Characterization of the proteinase | Biochemical Journal
Domestic protein purification instrument company on the characteristics of molecular sieve chromatography protein purification...
Patent US8821763 - Active material and method of manufacturing active material - Google Patents
Gel Permeation Chromatography Market 2017 : Shimadzu, TOSOH,
Fundamentals of Gel Permeation Chromatography (GPC) - Part 1 : Waters
Fundamentals of Gel Permeation Chromatography (GPC) - Part 1 : Waters
Interactions between collagen and C1q: their significance for rheumatology]. - Semantic Scholar
Gel permeation chromatography/ Size exclusion chromatography - ITN SNAL - Marie Curie Initial Training Network
Global Gel Permeation Chromatography (GPC) Sales Market (Detectors and Systems) Insights, Opportunity, Analysis, Market Shares...
Manual Gel Filtration using the PhyTip 5k Gel Filtration Columns from PhyNexus | SelectScience
Size Exclusion Chromatography | Materials Talks
Size Exclusion Chromatography Products, Reviews & Suppliers | SelectScience
Download Detection And Data Analysis In Size Exclusion Chromatography
Method of producing an electrode for non-aqueous electrolyte battery - Patent # 6361822 - PatentGenius
WHAT IS SIZE EXCLUSION/GEL PERMEATION CHROMATOGRAPHY (SEC/GPC)? - PolyAnalytik
HiLoad Superdex 75 pg preparative size exclusion chromatography columns | Cytiva
Gel Permeation Chromatography (GPC) | Systems & Detectors | Malvern Panalytical
2.3.1.7: Size-Exclusion Chromatography - Chemistry LibreTexts
Malvern OMNISEC GPC/SEC for measuring the distribution of molecular weights
Triple Detector | GPC | Viscotek 302-040
Ares G36 / KSK-1 Anti Jam Bügel
Sepax Technologies, Inc.
Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm | IDH Inhibitor idhinhibitor.com
Viral clearance by BioSAFE (Bioseparations Using Surfactant Aided Size Exclusion Chromatography)
- TU Delft Research Portal
Sepax Technologies, Inc.
Exclusion chromatography
zatra gold elution column
Invited Talks | Biophysical Resource
기본 레이아웃
기본 레이아웃
Лаборатория биомолекулярной ЯМР-спектроскопии - ИБХ РАН
Affinity purification and some molecular properties of human liver alkaline phosphatase | Biochemical Journal | Portland Press
Macrophage Suppression By a Low Molecular Weight Fraction of Murine Sp by Glenda Gayle Allen-Abbott
Global Gel Permeation Chromatography System Market 2018-2022
POWER STORAGE DEVICE AND METHOD FOR MANUFACTURING THE SAME - Patent application
US5569477A - Chewing gum containing vitamins or other active materials
- Google Patents
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? - QIAGEN
US20030008212A1 - Anode active material and nonaqueous electrolyte secondary battery
- Google Patents
Ul - Amino Acids - RR School Of Nursing
IMSEAR at HELLIS: Studies on immobilization of alfa- amylase to cyanogen bromide activated sephadex g 200
POSITIVE ACTIVE MATERIAL FOR LITHIUM SECONDARY BATTERY, METHOD OF PREPARING THE SAME, POSITIVE ELECTRODE FOR LITHIUM...
Generation of well-relaxed all-atom models of large molecular weight polymer melts: A hybrid particle-continuum approach based...
Ion-Exchange Chromatography Industry grow at a CAGR of 4.96% during the Size, Growth, Trends and Forecast to 2020 | Business
Purification of the enhancing factor from mouse intestines - Publications of the IAS Fellows
Size Exclusion Chromatography | Biopharmaceutical Manufacturing | MilliporeSigma
Handbook Of Size Exclusion Chromatography And Related Techniques, Buch. Chi-San Wu - ReadRate
Purification, Determination Molecular Weight and Study Kinetic
Properties of G6PD from Diabetes Patient | Open Access Journals
Ion Exchange Chromatography Research Paper - 306 Words
Transformation troubleshoot - Molecular Cloning
High Sensitivity Gel Permeation Chromatography for Agrochemical Analysis
Liverpool Life Sciences UTC Innovation Labs: Blood, sweat, tears and the milk of human kindness! Part 2
경신과학
A volatile solvent for high-performance gel-permeation chromatography of polypeptides | Biochemical Society Transactions
How To Fix Sources Of Error In Gel Filtration Chromatography Tutorial
커뮤니티 > 기초지식코너 > [HPLC] GPC - Gel Permeation...
Phenomenex HPLC Part: bioZen™ 1.8 µm SEC-3 , LC Column 100 x 4.6 mm, Ea (Part#: 00D-4772-E0)
M Columns - Columns and consumables - MACSmolecular - Products - Miltenyi Biotec - Belgique
Size Exclusion Chromatography (SEC) - Chromedia
Bioneer Pacific - AccuPrep® PCR Purification Kit
CA2005000377 METHOD AND APPARATUS FOR MOLD COMPONENT LOCKING USING ACTIVE MATERIAL ELEMENTS
a very unusual problem with ssDNA
FPLC - Superdex 200HR vs. Superose 12HR columns
Prepacked Columns Sepadextran™ 25 Medium SC | abtbeads.com
remove BSA from IgG prep - Protein and Proteomics
Determination of the molecular mass of M.EcoP1I by size | Open-i
Cellulose-derived carbons as a high performance anodic material for Na-ion battery | SpringerLink
Polyacrylamide gel electrophoresis
"Transmission-electron microscopic observations of freeze-etched polyacrylamide gels". Journal of Chromatography A. 166 (2): 563 ... Polyacrylamide gels are composed of a stacking gel and separating gel. Stacking gels have a higher porosity relative to the ... EtBr is used by simply adding it to the gel mixture. Once the gel has run, the gel may be viewed through the use of a photo- ... Preparing acrylamide gels[edit]. The gels typically consist of acrylamide, bisacrylamide, the optional denaturant (SDS or urea ...
Desalting and buffer exchange
Cytiva Animation of desalting using gel filtration chromatography Porath, J; Flodin P (13 June 1959). "Gel Filtration: A method ... These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size- ... exclusion chromatography. Desalting and buffer exchange are two of the most common gel filtration chromatography applications, ... There are a number of common formats for performing gel filtration for smaller (less than 4mL) volumes: Chromatography columns ...
RTI-31
... silica gel chromatography; (iv) DIBAL, toluene, -78 °C; (v) ClCOCOCl, DCM, -78 °C, DMSO, 30 min, TEA; (vi) Ph3P+CH2RBr−, n-BuLi ...
Pepsin
The fragments can be purified by gel filtration, ion exchange, or affinity chromatography.[38] ...
Procyanidin
Gel permeation chromatography (GPC) analysis allows to separate monomers from larger PCO molecules. ...
Comet assay
The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the ... Journal of Chromatography B 722(1-2): 225-254. doi:10.1016/S0378-4347(98)00313-2 (HTML abstract) ... The agarose is gelled at 4 °C and the coverslip removed. The agarose forms a matrix of carbohydrate fibres that encapsulate the ... The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet.[1][2] ...
QPNC-PAGE
Hjertén S (1963). ""Molecular-sieve" electrophoresis in cross-linked polyacrylamide gels". Journal of Chromatography A. 11: 66- ... As a result, the prepared gel is homogeneous (in terms of homogeneous distribution of cross-links throughout the gel sample), ... Overheating of the gel is impeded by internal cooling of the gel column and by generating a constant power (see figure ... "Improved separation of palladium species in biological matrices by using a combination of gel permeation chromatography and ...
Hinokinin
by silica gel column and counter-current chromatography". Journal of Chromatography B. 929: 6-10. doi:10.1016/j.jchromb.2013.04 ...
Mesoporous silicate
High-performance liquid chromatography Gas-liquid chromatography Silica gel Nanotechnology. ...
Bovine serum albumin
"Characterization of proteins and other macromolecules by agarose gel chromatography". Journal of Chromatography A. 152 (1): 21- ...
Gary Ackers
Professor Ackers invented agarose gel chromatography when he was a teenager. He went on the develop analytical gel ... chromatography methods for determinations of many important characteristics of water-soluble proteins; diffusion coefficient, ...
Casein
Lucey JA (2002). "Formation and Physical Properties of Milk Protein Gels". J. Dairy Sci. 85 (2): 281-94. doi:10.3168/jds.S0022- ... Kunz, C; Lönnerdal, B (1990). "Human-milk proteins: analysis of casein and casein subunits by anion-exchange chromatography, ... An attractive property of the casein molecule is its ability to form a gel or clot in the stomach, which makes it very ... gel electrophoresis, and specific staining methods". Am. J. Clin. Nutr. 51 (1): 37-46. doi:10.1093/ajcn/51.1.37. PMID 1688683. ...
Bachelor of Science in Human Biology
Techniques in experimental biochemistry: Colorimetry, spectrophotometry, spectrofluorometry, pH determination, gel ... electrophoresis, chromatography. THIRD PHASE: The third year was devoted to specialization in a chosen field of anatomy, ...
Gamma-glutamyltransferase
"A high performance gel filtration chromatography method for gamma-glutamyltransferase fraction analysis". Analytical ...
Stokes radius
Stokes radii are often determined experimentally by gel-permeation or gel-filtration chromatography. They are useful in ... Uversky, V.N. (1993). "Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which ...
Milos Novotny
Keene P. Dimick Award in Chromatography, Third International Symposium on Supercritical Fluid Chromatography Award for ... Novotny was recognized for the development of PAGE Polyacrylamide Gel-filled Capillaries for Capillary Electrophoresis in 1993 ... Tswett Award and Medal in Chromatography; American Chemical Society Award in Chromatography; ISCO Award in Biochemical ... In 1986, Novotny was given the Award in Chromatography from the American Chemical Society. Novotny received the ANACHEM award ...
Proanthocyanidin
Gel permeation chromatography (GPC) analysis allows separation of monomers from larger proanthocyanidin molecules. Monomers of ... galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography". ... Journal of Chromatography A. 1218 (43): 7804-12. doi:10.1016/j.chroma.2011.08.082. PMID 21930278. Engström, M. T.; Pälijärvi, M ...
Keratinase
1990) with the help of membrane ultra filtration and C-75 gel chromatography. He purified enzyme with 70-fold increased ... which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. The enzyme-activity was inhibited ...
Chiral derivatizing agent
Although Helmchen's postulates are specific for amides on silica gel using liquid chromatography, the postulates provide ... Chromatography also has many variations (e.g. HPLC, Gas Chromatography, flash chromatography) with a wide array of ... Diastereomers bind to surfaces (silica gel in normal phase chromatography) mainly with hydrogen bonding. Significant resolution ... In general, chromatography can be used to separate chiral compounds to bypass difficult crystallizations and/or to collect all ...
Germanium
Sol-Gel Hybrid Organic-Inorganic Coatings for Capillary Microextraction and Gas Chromatography". Anal. Chem. 79 (24): 9441-9451 ... the silica stationary phase in some gas chromatography columns can be replaced by GeO2. In recent years germanium has seen ...
Apamin
... was separated from the other compounds by gel filtration and ion exchange chromatography. Apamin is a polypeptide ...
Electrochromatography
It is a combination of size exclusion chromatography (gel filtration chromatography) and gel electrophoresis. These separation ... Chromatography Protein electrophoresis Electrofocusing Two-dimensional gel electrophoresis Temperature gradient gel ... The molecules are separated by size due to the gel filtration mechanism and by electrophoretic mobility due to the gel ... It is a combination of high-performance liquid chromatography and capillary electrophoresis. The capillaries is packed with ...
Diethylaminoethyl cellulose
... a type of column chromatography, for the separation and purification of proteins and nucleic acids. Gel matrix beads are ... "DEAE and CM Bio-Gel ® A Ion Exchange Gels Instruction Manual" (PDF). Bio-Rad. Retrieved 11 May 2016. "DEAE-Dextran" (PDF). GE ... Size-exclusion chromatography Stationary phase Rousseau, Ronald W.; Ferrell, James K.; Reardon, Robert F. (1984-06-01). " ... DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger. This exchange ...
Eye dropper
With a bit of skill, Pasteur pipettes may also be used for microscale column chromatography. With appropriately fine silica gel ... Microscale column chromatography The constriction toward the tip of the Pasteur pipettes may be plugged with a bit of tissue ... University of Colorado at Boulder, Procedure for Microscale Flash Column Chromatography. Accessed 1 Nov 2006. "Microscale ... the bulb may be squeezed for microscale flash column chromatography. Microscale distillation Pasteur pipettes can also be used ...
Ophanin
... was successfully isolated from O. Hannah venom by gel filtration and cation-exchange chromatography. Its molecular ...
Gradient copolymer
The composition can be determined by gel permeation chromatography(GPC) and nuclear magnetic resonance (NMR). Generally the ...
Mark-Houwink equation
In size-exclusion chromatography, such as gel permeation chromatography, the intrinsic viscosity of a polymer is directly ... "Gel Permeation Chromatography" Archived 2009-09-02 at the Wayback Machine California Polytechnic State University. 11 Dec. 2007 ... Therefore, by running several monodisperse samples of polymer in a gel permeation chromatograph (GPC), the values of K {\ ...
Taicatoxin
Sephadex G-50 gel filtration chromatography can be used, in the presence of high salt (1M NaCl) and alkaline conditions (pH = ... It migrates in beta-alanine-acetate-urea gel electrophoresis as a single compound. The phospholipase activity can be separated ... active complex was isolated by ion exchange chromatography through DE-Cellulose and two steps of Cm-Cellulose chromatography at ... by affinity chromatography, using a phospholipid analog (PC-Sepharose). The alpha-neurotoxin-like peptide can be separated from ...
Nespecifična monooksigenaza
... simultaneous quantitation of four aminopyrine metabolites by high-performance liquid chromatography". Arch. Biochem. Biophys. ... microsomal cytochrome P-450 by peptide mapping utilizing limited proteolysis in sodium dodecyl sulfate and analysis by gel ...
Pepper spray
High performance liquid chromatography (HPLC) is used to measure the amount of capsaicin and major capsaicinoids within pepper ... A study of five often-recommended treatments for skin pain (Maalox, 2% lidocaine gel, baby shampoo, milk, or water) concluded ...
Medical microbiology
For instance, traditional PCR techniques require the use of gel electrophoresis to visualize amplified DNA molecules after the ...
Ligandi sidumiskatse - Vikipeedia
1993). "[12] High-speed DNA sequencing by capillary gel electrophoresis". Methods in Enzymology. 218 (12): 154-172. ... Journal of Chromatography B. 829 (1-2): 1-25. ...
Protein
Various types of chromatography are then used to isolate the protein or proteins of interest based on properties such as ... most often after in-gel digestion), protein microarrays, which allow the detection of the relative levels of a large number of ... As a result, when the lysate is passed over a chromatography column containing nickel, the histidine residues ligate the nickel ... and chromatography; the advent of genetic engineering has made possible a number of methods to facilitate purification. Methods ...
Medical genetics
Southern blotting is an early technique basic on detection of fragments of DNA separated by size through gel electrophoresis ... Quantitative amino acid analysis is typically performed using the ninhydrin reaction, followed by liquid chromatography to ...
Germanium
Sol-Gel Hybrid Organic-Inorganic Coatings for Capillary Microextraction and Gas Chromatography". Anal. Chem. 79 (24): 9441-9451 ... the silica stationary phase in some gas chromatography columns can be replaced by GeO2.[73] ...
List of plasma physics articles
SEAgel, Safe Emulsion Agar gel. *Selected-ion flow-tube mass spectrometry. *Self-focusing ... Chromatography detector. *Chromo-Weibel instability. *Classical-map hypernetted-chain method. *Cnoidal wave ...
DNA sequencing
In these studies, automated sizing has proven to be more reproducible and precise than manual gel sizing.[43][44][45] ... were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. ... To visualize the fragments, the gel is exposed to X-ray film for autoradiography, yielding a series of dark bands each ... Single-nucleotide mutations in a fragment can be more easily detected with MS than by gel electrophoresis alone. MALDI-TOF MS ...
Molecular sieve
Regeneration temperatures range from 175 °C to 315 °C depending on molecular sieve type.[12] In contrast, silica gel can be ... Some molecular sieves are used in chromatography, a separation technique that sorts molecules based on their size. Other ... A sealed canister, commonly filled with silica gel and other molecular sieves used as desiccant in drug containers to keep ... However, some types of silica gel will "pop" when exposed to enough water. This is caused by breakage of the silica spheres ...
Nuclear magnetic resonance spectroscopy of proteins
... or preparation of the sample in a stretched polyacrylamide gel. This creates a local environment that favours certain ...
Deoxyribozyme
A common set-up for this is a biotin tag with a streptavidin affinity column.[21][22] Gel electrophoresis based separation can ... As an example, the separation step for ribonucleotide cleavage often utilizes affinity chromatography, in which a biological ... weight of strands upon the cleavage reaction is enough to cause a shift in the location of the reactive strands on the gel.[22] ...
Dielectrophoresis
Journal of Liquid Chromatography & Related Technologies. 20 (16-17): 2857-2872. doi:10.1080/10826079708005597.. ... Gel electrophoresis of nucleic acids. *Gel electrophoresis of proteins. *Serum protein electrophoresis ...
Ion chromatography
Agarose gel based medium contain large pores as well but their substitution ability is lower in comparison to dextrans. The ... Size exclusion chromatography. Micellar liquid chromatography. Ion chromatography (or ion-exchange chromatography) separates ... Anion exchange chromatography retains anions using positively charged functional group: R-X. +. A. −. +. M. +. B. −. ⇄. R-X. + ... or in chromatography columns. Thin layer chromatography or column chromatography share similarities in that they both act ...
Ultraviolet
"UVC LEDs Enhance Chromatography Applications - GEN". GEN. Archived from the original on 4 November 2016.. ... "gels". ... sources to replace deuterium lamps in liquid chromatography ...
X-ray crystallography
... forming a useless dust or amorphous gel on the bottom of the container. Crystal growth in solution is characterized by two ...
Mass spectrometry
Similar to gas chromatography MS (GC-MS), liquid chromatography-mass spectrometry (LC/MS or LC-MS) separates compounds ... either in solution or in gel after electrophoretic separation. Other proteolytic agents are also used. The collection of ... The duty cycle of IMS is short relative to liquid chromatography or gas chromatography separations and can thus be coupled to ... Eiceman, G.A. (2000). Gas Chromatography. In R.A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory, and ...
C1QBP
33 kDa on SDS-PAGE gel under both reducing and nonreducing condition but migrates as a trimer on size-exclusion chromatography ... gel filtration).[8] Protein structure[edit]. The crystal structure of C1QBP at 2.25 Å resolution shows a homotrimeric ring ...
Drug discovery
Chemical compounds exist in nature as mixtures, so the combination of liquid chromatography and mass spectrometry (LC-MS) is ... "New scale-down methodology from commercial to lab scale to optimize plant-derived soft gel capsule formulations on a commercial ...
Membrane technology
... another is measurement of the cut-off by gel permeation chromatography. These methods are used mainly to measure membranes for ...
Mass spectrometry
Similar to gas chromatography MS (GC-MS), liquid chromatography-mass spectrometry (LC/MS or LC-MS) separates compounds ... either in solution or in gel after electrophoretic separation. Other proteolytic agents are also used. The collection of ... Main article: Gas chromatography-mass spectrometry. A common combination is gas chromatography-mass spectrometry (GC/MS or GC- ... The duty cycle of IMS is short relative to liquid chromatography or gas chromatography separations and can thus be coupled to ...
Chromatography - Simple English Wikipedia, the free encyclopedia
The most common stationary phase for column chromatography is silica gel, followed by alumina. Cellulose powder has been used ... Thin layer chromatography[change , change source]. Main article: Thin layer chromatography. Thin layer chromatography (TLCC) is ... Column chromatography[change , change source]. Column chromatography separates compounds using many chemical actions between ... Double-way paper chromatography, also called 'two-dimensional chromatography', uses two solvents and rotates the paper 90° in ...
Alkaloid
... followed by silica-gel chromatography purification.[185] Tracking and dosing the extracted solenopsin ant alkaloids has been ... or solvent extractions followed by silica-gel column chromatography.[7] Alkaloids have a wide range of pharmacological ... "Gas-chromatography and UV-spectroscopy of Hymenoptera venoms obtained by trivial centrifugation". Data in Brief. 18: 992-998. ...
Gel pen
Because manufacturers change their ink formulas slightly from year to year, thin-layer chromatography (TLC) can be used on ink ... A gel pen uses ink in which pigment is suspended in a water-based gel.[1] Because the ink is thick and opaque, it shows up more ... Many gel inks are water resistant, and are not washed away by water once the ink has dried. Fraud resistant gel ink is ... Another trait of gel ink is that it resists an analytical method commonly used to assess the potential age of ink for forensic ...
Dehydroascorbic acid
"Gel composition for reduction of gingival inflammation and retardation of dental plaque." U.S Patent 5,298,237, filed Jan.24, ... "Analysis of the transformation products of dehydro-L-ascorbic acid by ion-pairing high-performance liquid chromatography". ...
Materials science
... chromatography, thermal analysis, electron microscope analysis, etc. Structure is studied at various levels, as detailed below ...
Ion chromatography
Agarose gel based medium contain large pores as well but their substitution ability is lower in comparison to dextrans. The ... Micellar liquid chromatography. Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ... High performance liquid chromatography. Aqueous Normal Phase Chromatography. Size exclusion chromatography. ... Anion exchange chromatography retains anions using positively charged functional group: R-X. +. A. −. +. M. +. B. −. ⇄. R-X. + ...
Affinity chromatography
Another use for affinity chromatography is the purification of specific proteins using a gel matrix that is unique to a ... Weak affinity chromatography[edit]. Weak affinity chromatography[28] (WAC) is an affinity chromatography technique for affinity ... Boronate affinity chromatography[edit]. Boronate affinity chromatography consists of using boronic acid or boronates to elute ... Lectin affinity chromatography is a form of affinity chromatography where lectins are used to separate components within the ...
Polymer
Size-exclusion chromatography (also called gel permeation chromatography), sometimes coupled with static light scattering, can ...
High-fructose corn syrup
Some is processed into HFCS 90 by liquid chromatography, then mixed with HFCS 42 to form HFCS 55. The enzymes used in the ...
Use Of Silica Gel In Flash Chromatography
... flash chromatography is highly popular. Flash chromatography is also known as ... Among all the chromatography methods being used to separate different components of any given mixture, ... Use of Silica Gel in Flash Chromatography column-chromatography.com Flash chromatography is also known as "medium pressure ... Use Of Silica Gel In Flash Chromatography Among all the chromatography methods being used to separate different components of ...
Gel Permeation Chromatography Market 2017 : Shimadzu, TOSOH,
... Waters, Malvern, Agilent Technologies - published on openPR.com ... Gel Permeation Chromatography Market - Global Industry Analysis 2024 Gel permeation chromatography (GPC) or size exclusion ... The Gel Permeation Chromatography research study covers each and every aspect of the Gel Permeation Chromatography market ... Gel Permeation Chromatography Market Research Report. A market study based on the " Gel Permeation Chromatography Market " ...
Fundamentals of Gel Permeation Chromatography (GPC) - Part 1 : Waters
This module covers the first part of the fundamentals of Waters Gel Permeation Chromatography (GPC). Topics included in this ... module: monomers and polymers, polymer analysis techniques and applications, size-exclusion chromatography, and detectors. ... This module covers the first part of the fundamentals of Waters Gel Permeation Chromatography (GPC). Topics included in this ... This module covers the first part of the fundamentals of Waters Gel Permeation Chromatography (GPC). Topics included in this ...
Gel Permeation Chromatography Training | Testa Analytical Solutions eK | May 2020 | Photonics.com
A series of online training sessions on gel permeation chromatography have been announced by Testa Analytical Solutions EK. ... the trainings are focused on gel permeation chromatography, which is a type of size-exclusion chromatography that separates ... BERLIN, May 20, 2020 - A series of online training sessions on gel permeation chromatography have been announced by Testa ...
Open tubular liquid chromatography using a sol-gel derived stationary phase | SpringerLink
... was fabricated by the sol-gel process and coated onto the inner walls of fused silica capillaries. The coating was used as... ... Column liquid chromatography Open tubular columns Sol-gel coatings Laser-induced fluorescence detection ... The coating was used as the stationary phase for reversed-phase open tubular liquid chromatography (OTLC). The sol-gel process ... Solute selectivity of the sol-gel derived stationary phase was found to be similar to the selectivity of HPLC monomeric-type ...
Silica Gel for Flash Chromatography by Priya Singh
... which is also called as medium pressure chromatography, was developed by Clark Still from Columbia University in the year 1978 ... as an option to gravity column chromatography. Les... ... Silica Gel for Flash Chromatography. by Priya Singh An Impurity ... The size of the silica gel products varies in the mesh sizes of Silica Gel 60-120 Mesh, Silica Gel 60-200 Mesh, Silica Gel 70- ... Benefits of Flash Chromatography:. Silica Gel for Flash Chromatography have its biggest advantages are size of the molecules ...
WikiGenes - Chromatography, Gel
Gene context of Chromatography, Gel. *By gel filtration chromatography, one of the mutant Cdc28 proteins exhibited kinase ... Associations of Chromatography, Gel with chemical compounds. *The protein has been purified to homogeneity by gel filtration ... Anatomical context of Chromatography, Gel. *Using a procedure involving DEAE-cellulose, hydroxyapatite and gel filtration ... Gel. *Fibronectin was resolved from activity A by gelatin affinity chromatography or gel filtration [36]. ...
Gel filtration Chromatography - Protein and Proteomics
Gel filtration Chromatography - (Apr/04/2011 ). Hello friends,. I am doing gel filtration chromatography(GFC) to seperate a 12 ... Why is my gel bed getting compressed is it beacuse of the flow rate. Is it normal? Or should I reduce the flow rate further.(As ... most gel filtration matrices will compress under flow, especially if pumped.. after packing and prior to running a sample the ... in case you dont already have it i am attaching the gel filtration handbook from ge healthcare.. ...
Desalting and Gel Filtration Chromatography | Thermo Fisher Scientific - ZA
affinity chromatography. Gel filtration chromatography is useful for many of the same purposes as dialysis, because both ... Gravity-flow columns and chromatography cartridges use head-pressure from a buffer-chase to push the sample through the gel ... Desalting and buffer exchange are two of the most widely used gel filtration chromatography applications, and both can be ... Finally, in contrast to dialysis, gel filtration chromatography allows the contaminating material to be removed in a relatively ...
ACROS OrganicsSilica Gel, for Column Chromatography, 0.060-0.200mm, 60
| Fisher Scientific
Gel permeation chromatography - Wikipedia
Helmut, D. Gel Chromatography, Gel Filtration, Gel Permeation, Molecular Sieves: A Laboratory Handbook; Springer-Verlag, 1969. ... Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of ... Gel permeation chromatography is conducted almost exclusively in chromatography columns. The experimental design is not much ... The term gel permeation chromatography can be traced back to J.C. Moore of the Dow Chemical Company who investigated the ...
Gel Permeation Chromatography
Waters GPC-1 (1515 HPLC Pump & Waters 717Plus Autoinjector)* Mobile phase: Tetrahydrofuran (THF) Column set: Jordi Gel ... Gel Permeation Chromatography. * GPC set-up may change according to need. Please consult with lab manager for current set-up ... Column set: Jordi Gel fluorinated DVB columns (1-100K, 2-10K & 1-500Å). Lower molecular weight column set available.. Injection ... Column set: Two mixed bed Jordi Gel DVB columns. Injection volume: 10µl - 200µl. Optional 2400 µl sample holding loop available ...
An Examination of the Hexane Extract of Flue-Cured Tobacco Involving Gel Permeation Chromatography | RTI
Silica gel high-purity grade, 90Å, 70-230 mesh, for column chromatography | 112926-00-8
... for column chromatography; CAS Number: 112926-00-8; EC Number: 231-545-4; find Supelco-60745 MSDS, related peer-reviewed papers ... Silica gel is a rigid 3D network of colloidal silica. It is exclusively used as a support material for the active titanium (III ... Silica gel is classified as below:. a. Aquagel - pores are filled with water. b. Xerogel - by the process of evaporation, ... Silica gel allows a controlled fragmentation leading to form uniform polymer particles with narrow particle size distribution ...
Global Gel Permeation Chromatography System Market 2018-2022
About Gel Permeation Chromatography GPC is an analytical method used for the separation - Market research report and industry ... Global Gel Permeation Chromatography System Market 2018-2022 Global Gel Permeation Chromatography System Market 2018-2022 ... Global Gel Permeation Chromatography System Market 2018-2022. About Gel Permeation Chromatography. GPC is an analytical method ... Global Gel Permeation Chromatography System Market 2018-2022. Lowest Prices Guaranteed. Length. Publisher. Published Date. SKU ...
High performance liquid chromatography (HPLC) and Gel permeation chromatography (GPC) :: Scientific and Technical Services at...
Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography
We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic ... Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography ... Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of ...
Silica gel high-purity grade (puriss), pore size 60 Å, 70-230 mesh, for column chromatography | 112926-00-8
... for column chromatography; CAS Number: 112926-00-8; EC Number: 231-545-4; find Supelco-60740 MSDS, related peer-reviewed papers ... Silica gel high-purity grade (puriss), pore size 60 Å, 70-230 mesh, ... Silica gel is a rigid 3D network of colloidal silica. It is exclusively used as a support material for the active titanium (III ... Silica gel is classified as below:. a. Aquagel - pores are filled with water. b. Xerogel - by the process of evaporation, ...
GPC Quattro gel permeation chromatography (GPC) Instrument from Pickering Laboratories, Inc. | SelectScience
Read independent reviews on GPC Quattro gel permeation chromatography (GPC) Instrument from Pickering Laboratories, Inc. on ... GPC Quattro gel permeation chromatography (GPC) Instrument. GPC Quattro gel permeation chromatography (GPC) Instrument by ... GPC Quattro gel permeation chromatography (GPC) Instrument. Manufacturer Pickering Laboratories, Inc.. Be the first to review ...
Optimizing Sample Preparation for Capillary Gel Electrophoresis | Chromatography Online
... experiments performed to optimize a standard quality-control method for protein purity evaluation using reducing capillary gel ... Liquid Chromatography (LC/HPLC)Gas Chromatography (GC)Sample PreparationMass SpectrometryPharmaceutical AnalysisEnvironmental ... and Flow-Modulation of Two-Dimensional GC-MSChromatography Advances at Virtual EAS 2020. ... GCGC-MSGeneralIndustrialLCLC-MSMedical/BiologicalMisc TechniquesPharmaceuticalsPolymersSample PrepSize-Exclusion Chromatography ...
SiliaFlash - Irregular Silica Gels for Chromatography
Chromatography * Quick Overview Of Column Chromatography * Reversed-Phase Chromatography: General Introduction For Improved ... SiliaSphere - Spherical Silica Gels * SiliaSphere - for Analytical Chromatography * Bare Silica * 60 Å Bare Spherical Silica ... SiliaFlash® - Irregular Silica Gels for Chromatography. With pore diameters ranging from 30 to 300 Ångström (Å) and particle ... Our silica gels are ideal for preparative chromatography, from laboratory to pilot-plant processes and production scale. In ...
Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to characterize...
Article Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to ... Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to characterize ... Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy and gel-permeating chromatography (GPC) were ... were found for Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to ...
Global Gel Permeation Chromatography (GPC) Sales Market (Detectors and Systems) Insights, Opportunity, Analysis, Market Shares...
... the Global Gel Permeation Chromatography (GPC) Market 2017 is valued at USD XX million in 2016 and is expected to reach USD XX ... 4.3 China Gel Permeation Chromatography (GPC) Sales Volume and Market Share by Type. 4.4 China Gel Permeation Chromatography ( ... 6.3 Japan Gel Permeation Chromatography (GPC) Sales Volume and Market Share by Type. 6.4 Japan Gel Permeation Chromatography ( ... 8.3 India Gel Permeation Chromatography (GPC) Sales Volume and Market Share by Type. 8.4 India Gel Permeation Chromatography ( ...
Storing an Ion-Exchange Chromatography Gel in Dilute Alkali During Recycling Improves Cleaning
... enhances gel cleaning. More material is removed from the gel during column recycling, and carry over of material from one cycle ... Central Public Health Laboratory and Zain Moola This case study shows that storing an ion-exchange chromatography gel in dilute ... Storing an Ion-Exchange Chromatography Gel in Dilute Alkali During Recycling Improves Cleaning. @media screen and (max-width: ... Central Public Health Laboratory and Zain Moola This case study shows that storing an ion-exchange chromatography gel in dilute ...
Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for...
Conventional capillary liquid chromatography/mass spectrometry (LC/MS) typically employs low microl/min flow rates with gas/ ... Conventional capillary liquid chromatography/mass spectrometry (LC/MS) typically employs low microl/min flow rates with gas/ ... at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography- ... low nanogram sensitivity was attained for proteins derived from in-gel-digested silver-stained bands from 1-D and 2-D gels. ...
BANGKAI Thin Layer Chromatography Silica Gel SIO2 - QINGDAO BANGKAI HI-TECH MATERIALS CO.,LTD - ecplaza.net
A volatile solvent for high-performance gel-permeation chromatography of polypeptides | Biochemical Society Transactions
DIFFUSIONAL PHENOMENA IN FLOWING POLYMER SOLUTIONS. I. THEORY OF RESOLUTION DISPERSION IN GEL PERMEATION CHROMATOGRAPHY.
Thin-layer chromatoElectrophoresisHPLCExchange chromatographyFlash ChromatographyFiltrationAffinitySeparation2017Analytical ChromatographyStationaryKnown as size exclusion chroGlobal Gel Permeation ChrProteinsAdsorptionSilica gel adsorbentPoreCompoundsFractionationAmount of silica gelMeshPreparative chromatographyPolymerAgaroseElutionColumnChromatographicLiquidMethodsSolventPurificationAdsorbentsAnalytes2016GravityExclusionColumnsSizePolyolefinsSeparationsSephadexQuality silicaOptimizePorousMolecular weight
Thin-layer chromato2
- There are various techniques used in the process of chromatography like plane chromatography, paper chromatography, thin layer chromatography, column chromatography etc. (microscopiaiwm.com)
- This chromatographic technique can be performed wither by column or by thin layer chromatography techniques. (microscopiaiwm.com)
Electrophoresis14
- Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. (go.jp)
- This installment of 'Directions in Discovery' describes experiments performed to optimize a standard quality-control method for protein purity evaluation using reducing capillary gel electrophoresis. (chromatographyonline.com)
- Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. (uniprot.org)
- Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from Shewanella putrefaciens separated by two-dimensional polyacrylamide gel electrophoresis. (ugent.be)
- In this context, we undertook improvements to the conformation sensitive gel electrophoresis (CSGE) method which provides a cost-effective approach to mutation detection and compared the results with scanning carried out using denaturing high performance liquid chromatography (DHPLC) which utilises a dedicated analyser. (ac.ir)
- We used fast protein liquid chromatography (FPLC) with an anion-exchange column (Mono-Q) and polyacrylamide gradient gel electrophoresis techniques to analyze the casein subunit composition. (elsevier.com)
- Kunz, C & Lönnerdal, B 1990, ' Human-milk proteins: Analysis of casein and casein subunits by anion-exchange chromatography, gel electrophoresis, and specific staining methods ', American Journal of Clinical Nutrition , vol. 51, no. 1, pp. 37-46. (elsevier.com)
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that the purified alkaline protease is a monomeric enzyme with a molecular mass of ~33 kDa. (ispub.com)
- This strategy is based on polyacrylamide gel electrophoresis separation of protein isoforms, mass spectrometry (MS) and MS n analyses of intact proteins, and tandem MS analyses of proteolytic peptides. (mcponline.org)
- We believe that this strategy, which combines two-dimensional gel electrophoresis and mass spectrometric analyses of gel-eluted intact proteins using a benchtop ion trap mass spectrometer, represents a promising approach in proteomics. (mcponline.org)
- In this approach, protein identification is achieved after protein separation by one- or two-dimensional (1D or 2D) polyacrylamide gel electrophoresis followed by protein digestion usually with trypsin, analyses of the resulting peptide mixture using various MS approaches, and data base search. (mcponline.org)
- To overcome limitations of 2D gel electrophoresis, alternative techniques based on multidimensional liquid chromatography have been recently developed ( 1 , 2 ). (mcponline.org)
- The most efficient technique to separate protein isoforms thus remains 2D gel electrophoresis. (mcponline.org)
- Either technique should not be confused with gel electrophoresis, where an electric field is used to "pull" or "push" molecules through the gel depending on their electrical charges. (wikipedia.org)
HPLC7
- Solute selectivity of the sol-gel derived stationary phase was found to be similar to the selectivity of HPLC monomeric-type phases. (springer.com)
- We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. (go.jp)
- With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods. (separationsnow.com)
- Our silica gel chromatography media meets the requirements of high performance liquid chromatography (HPLC) analysis in the laboratory and preparative separations in industry scale. (epruibiotech.com)
- HELIX Chromatography is offering a recycling program for universities and pharmaceutical, chemical, environmental, and food companies wishing to recycle their analytical and preparative HPLC columns. (helixchrom.com)
- The sol-gel-immobilized Tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy) 3 2+ ] electrogenerated chemiluminescence (ECL) sensor was applied to the reversed-phase high-performance liquid chromatography (HPLC) determination of phenothiazine derivatives (promazine, chlorpromazine, triflupromazine, thioridazine, and trifluoperazine) and erythromycin in human urine samples. (elsevier.com)
- High-performance liquid chromatography (HPLC) as with GC, is a universal separation technique applicable to complex mixtures. (ineos.com)
Exchange chromatography5
- by Jane Turton, Central Public Health Laboratory and Zain Moola This case study shows that storing an ion-exchange chromatography gel in dilute alkalai, followed by removal of the solubized material, enhances gel cleaning. (biopharminternational.com)
- Based on these techniques, further different types of chromatographic procedures have been curated which include adsorption chromatography, partition chromatography, gel permeation chromatography, ion exchange chromatography and affinity chromatography. (microscopiaiwm.com)
- It is of different kinds including gas chromatography, ion exchange chromatography, liquid chromatography, gel permeation chromatography, and affinity chromatography. (smp-ind.com)
- Health Sciences "Ion Exchange Chromatography, Principles, and Methods" Handbook. (alignedstrategy.com)
- An alkaline protease was produced and partially purified from a new strain of Aspergillus oryzae by using two chromatographies i.e. ion exchange chromatography on CM-Sephadex C-50 and gel filtration on Sephadex G-100 yielding an active major protein peak with ~29.29 purification fold. (ispub.com)
Flash Chromatography6
- Among all the chromatography methods being used to separate different components of any given mixture, flash chromatography is highly popular. (buzzfeed.com)
- Flash chromatography, which is also called as medium pressure chromatography, was developed by Clark Still from Columbia University in the year 1978, as an option to gravity column chromatography. (apsense.com)
- Silica gel is the best adsorbents for flash chromatography in the solid phase that is packed tightly at the bottom of the glass cylinder so the solution will purify the raw material in the process. (apsense.com)
- Silica Gel for Flash Chromatography have its biggest advantages are size of the molecules can be known for separating and purifying the reaction mixture in the fast manner. (apsense.com)
- The size of the silica gel for Flash Chromatography varies according to the size of the impurities that has to be removed and the size can be minimum for fast recovery of the minerals. (apsense.com)
- Smaller particles (higher mesh values) are used for flash chromatography, larger particles (lower mesh values) are used for gravity chromatography. (dynamicadsorbents.com)
Filtration36
- Hello friends, I am doing gel filtration chromatography(GFC) to seperate a 12 kDa protein and its dimer approx 24 kDa. (protocol-online.org)
- most gel filtration matrices will compress under flow, especially if pumped. (protocol-online.org)
- in case you don't already have it i am attaching the gel filtration handbook from ge healthcare. (protocol-online.org)
- Desalting and buffer exchange use gel filtration chromatography to separate soluble macromolecules from smaller molecules. (thermofisher.com)
- Desalting and buffer exchange are two of the most widely used gel filtration chromatography applications, and both can be performed using the same materials. (thermofisher.com)
- In desalting and buffer exchange, the macromolecular components of a sample are recovered in the buffer used to pre-equilibrate the gel-filtration matrix through which the sample is processed. (thermofisher.com)
- The method is commonly referred to as desalting when the goal is to remove buffer salts from a sample in exchange for water (with water used to pre-equilibrate the gel-filtration resin). (thermofisher.com)
- Gel filtration chromatography is useful for many of the same purposes as dialysis, because both methods are based on similar ranges of molecular weight cut-off (MWCO) limits that exclude molecules based on size. (thermofisher.com)
- Compared to dialysis, gel filtration has the advantage of speed (a few minutes vs. hours for dialysis), which is necessary in certain experimental situations. (thermofisher.com)
- Also, gel filtration is compatible with organic and other solvents that dissolve or otherwise compromise the integrity of dialysis membranes. (thermofisher.com)
- Finally, in contrast to dialysis, gel filtration chromatography allows the contaminating material to be removed in a relatively small volume (and left on the column), an important feature when working with toxic or radioactive substances. (thermofisher.com)
- Gel filtration separates molecules of different dimensions based on their relative abilities to penetrate into a suitable stationary phase or chromatographic resin. (thermofisher.com)
- When a sample solution is passed through a column of packed gel filtration resin, small molecules in the sample (buffer salts, small molecules, etc.) enter the pores of resin beads that they encounter and are forced to follow a circuitous path before later exiting the beads. (thermofisher.com)
- When an appropriate gel filtration resin is used, many different classes of macromolecules can be separated from buffer salts, unconjugated labeling reagents and other molecules to achieve rapid purification before downstream applications. (thermofisher.com)
- Silica gel may be used in separation of single-walled carbon nanotube (SWNT) by simple filtration. (sigmaaldrich.com)
- In this report, procedures are discussed for the enrichment of single-walled carbon nanotube (SWNT) types by simple filtration of the functionalized SWNTs through silica gel. (sigmaaldrich.com)
- It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. (separationsnow.com)
- We have examined the effects of GH and estrogen status on GHBP measured in 24-h pool samples and compared results obtained from gel filtration and immunoprecipitation. (edu.au)
- This method has various other names such as molecular sieve, gel filtration and molecular exclusion chromatography. (microscopiaiwm.com)
- Gel permeation chromatography is also called as gel filtration or size exclusion chromatography. (microbenotes.com)
- Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. (mdpi.com)
- In offering help with writing a gel-filtration chromatography report, at our firm , we are aware that the separation of biological molecules is based on the differences in the size and /or shape of the analyte molecules. (customwritingbay.com)
- A standard filtration chromatography lab report should be detailed and straight forward and also present results using appropriate tables and graphs. (customwritingbay.com)
- Our custom gel-filtration chromatography lab report writers are familiar with the fact that gel filtration chromatography is the simplest and mildest of all chromatography techniques. (customwritingbay.com)
- In offering help with chromatography lab reports , our writers are familiar with the fact that gel filtration is a technique that is well suited to the handling of biomolecules that are sensitive to the changes in pH, the concentration of metal ions, cofactors and harsh environmental conditions. (customwritingbay.com)
- Molecules can be separated by their size using gel-filtration chromatography. (customwritingbay.com)
- Gel filtration chromatography is also used to purify proteins, polysaccharides, and nucleic acids. (customwritingbay.com)
- It is essential to note that your accurate results will not be useful if you write a substandard gel-filtration lab report. (customwritingbay.com)
- Looking for quality assistance with writing a lab report on gel-filtration chromatography from qualified people can boost your chances of achieving a good grade in lab report writing. (customwritingbay.com)
- Proteins and peptides are separated from a mixture based on the size of their molecules using gel-filtration chromatography. (customwritingbay.com)
- Gel-filtration column chromatography showed that, like the wild-type GC-c, the mutated protein also formed a high-molecular-weight complex. (ahajournals.org)
- 2. Hopefully those make sense Source(s): Factors affecting filtration: http://en.wikipedia.org/wiki/Size-exclus. (alignedstrategy.com)
- Would you be able to separate catalase and haemoglobin by the technique of gel-filtration? (alignedstrategy.com)
- Gel-filtration analysis with antibodies against CSN subunits 4, 5 and 7 revealed that these proteins act as a complex in Drosophila that is similar in size to the plant and mammalian complexes. (biologists.org)
- Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. (wikipedia.org)
- The main application of gel-filtration chromatography is the fractionation of proteins and other water-soluble polymers, while gel permeation chromatography is used to analyze the molecular weight distribution of organic-soluble polymers. (wikipedia.org)
Affinity4
- Other desirable properties of the gel forming agent are the absence of ionizing groups and, in a given solvent, low affinity for the substances to be separated. (wikipedia.org)
- Silica gel has a very strong affinity for water, which gives it an adsorption capacity of over 40% of its own weight at 100% relative humidity. (dynamicadsorbents.com)
- Title: Affinity Chromatography Obje Experiment 6 Erik, Odom Page5 / 6 The sources of error in this experiment could have come. (alignedstrategy.com)
- The two forms of mMBL could be separated both by ion-exchange and carbohydrate-affinity chromatography. (jimmunol.org)
Separation17
- Flash column chromatography is the quick substances that are helpful for removing or separation of contamination from the raw materials. (apsense.com)
- The pore size of a gel must be carefully controlled in order to be able to apply the gel to a given separation. (wikipedia.org)
- Commercial gels like PLgel & Styragel (cross-linked polystyrene-divinylbenzene), LH-20 (hydroxypropylated Sephadex), Bio-Gel (cross-linked polyacrylamide), HW-20 & HW-40 (hydroxylated methacrylic polymer), agarose gel and are often used based on different separation requirements. (wikipedia.org)
- Separation of single-walled carbon nanotubes on silica gel. (sigmaaldrich.com)
- Size-exclusion chromatography is a separation mode that separates the molecules by size, and falls into two types: non-aqueous GPC and aqueous GFC. (shimadzu.com.au)
- Silica gel for the separation is used in different methods of chromatography. (column-chromatography.com)
- A long column filled with gel beads or porous glass granules is allowed to attain equilibrium with a solvent which is suitable for the separation of the desired compound or molecule. (microscopiaiwm.com)
- Column chromatography is the ideal method of chromatography for purification and separation. (sorbeadindia.com)
- The process of column chromatography is the oldest and the most common technique f or the separation of complex mixtures packed in a column. (sorbeadindia.com)
- Analytical chromatography i s a simple method of chromatography with faster and cost effective separation. (sorbeadindia.com)
- After preparative process, improving impurity profile and speed of separation use semi-preparative chromatography. (sorbeadindia.com)
- The MCI-Gel® product line offers analytical and preparative columns for protein purification, sugars and oligosacharides separations, and separation and purification of pharmaceuticals. (biokal.com)
- For the separation of sugars and oligosacharides MCI-Gel ® offers analytical and semi-preparative columns analogue to the industrial equivalent DIAION™ UBK-resins. (biokal.com)
- Gas chromatography (GC) is a universal separation technique applicable to complex mixtures. (ineos.com)
- This chromatographic technique uses different mobile phases and columns to achieve the necessary separation of polar, thermally labile, non-volatile and other analytes that cannot be analyzed by gas chromatography. (ineos.com)
- For industrial applications, immobilization of the enzyme in gel or solid supports may offer several advantages such as repeated use of the enzyme, ease of product separation and improvement of enzyme stability [ 5 , 6 ]. (ispub.com)
- With size exclusion chromatography, there are short and well-defined separation times and narrow bands, which lead to good sensitivity. (wikipedia.org)
20174
- The global Gel Permeation Chromatography market 2017 is also analysed on the basis of product pricing, Gel Permeation Chromatography production volume, data regarding demand and Gel Permeation Chromatography supply, and the revenue garnered by the product. (openpr.com)
- The 2017/18 Waters Quality Parts, Chromatography Columns and Supplies Catalog is available! (waters.com)
- In this report, the Global Gel Permeation Chromatography (GPC) Market 2017 is valued at USD XX million in 2016 and is expected to reach USD XX million by the end of 2022, growing at a CAGR of XX% between 2016 and 2022. (medgadget.com)
- The worldwide market for Gel Permeation Chromatography (GPC) is expected to grow at a CAGR of roughly 2.4% over the next five years, will reach 130 million US$ in 2023, from 120 million US$ in 2017, according to a new GIR (Global Info Research) study. (reportsnreports.com)
Analytical Chromatography2
- The starting phase of chromatography is analytical chromatography with little amount of silica gel mesh 60-120 size by using analytical column packaging, to analysis how many percentage of mixture is purify. (sorbeadindia.com)
- As a highly selective adsorbent, Florisil is frequently employed in many areas of preparative and analytical chromatography. (rosesci.com)
Stationary9
- The coating was used as the stationary phase for reversed-phase open tubular liquid chromatography (OTLC). (springer.com)
- The sol-gel process facilitated the fabrication of a porous silica glass layer with the stationary phase in one single step. (springer.com)
- Gels are used as stationary phase for GPC. (wikipedia.org)
- It is an effective chromatography technique in which stationary phase adsorbs the unwanted compounds and other impurities using silica gel and activated aluminum oxide powder as powerful adsorbents. (column-chromatography.com)
- In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc. (microbenotes.com)
- This catalog lists reference materials and stationary phases for liquid chromatography of synthetic and natural macromolecules and proteins. (chemeurope.com)
- It is a technique in which the stationary phase is solid adsorbents like silica gel and activated alumina powder and the mobile phase is a liquid. (sorbeadindia.com)
- Chromatography is a technology by which a mixture of chemicals are separated by its components between two phases like stationary phase which is remain fixed in placed using two adsorbents such as silica gel and activated alumina, while as mobile phase is another method which is slowly movable and flows down through the column by either gravitational forces or external pressure into the column. (sorbeadindia.com)
- The shape and size of the compound (eluent) determine how the compound interacts with the gel (stationary phase). (wikipedia.org)
Known as size exclusion chro1
- Gel permeation chromatography (GPC), also known as size exclusion chromatography, is an analytical technique that determines the molecular weight distribution (MWD) of a polymer by virtue of its hydrodynamic volume. (ineos.com)
Global Gel Permeation Chr4
- Gel Permeation Chromatography Market Research 2018 Globel Info Research Recently added detailed market study on the "Global Gel Permeation Chromatography Market" Research Report 2018-2025 which provides an outlook of current market value of Gel Permeation Chromatography Market as well as the expected forecast of Rate on Investment (ROI) with growing CAGR of XX% in Gel Permeation Chromatography Market by the end of 2025. (openpr.com)
- Fragmented Market to provide Opportunities for Small Players Presently, the global gel permeation chromatography market is highly fragmented and competitive owing to the presence of many established players in the market. (openpr.com)
- Technavio's analysts forecast the Global Gel Permeation Chromatography Devices Market to grow at a CAGR of 6.63% during the period 2018-2022. (marketresearch.com)
- There are 15 Chapters to deeply display the global Gel Permeation Chromatography (GPC) market. (reportsnreports.com)
Proteins3
- For analysis of proteins/peptides in solution, low femtomole sensitivity has been achieved (attomoles for selected-ion monitoring), while low nanogram sensitivity was attained for proteins derived from in-gel-digested silver-stained bands from 1-D and 2-D gels. (nih.gov)
- We are developing large scale process chromatography for improving purity in various biologics such as proteins, virus, vitamins, hormones, anti-bodies. (sorbeadindia.com)
- Gel documentation (gel doc) and image analysis of fluorescent proteins, DNA, gels, western blots, microarrays and in colony counting is achieved with workstation systems and 1D or 2D software. (selectscience.net)
Adsorption2
- Silica gel adsorbs relatively large quantities of water which was explained on the basis of multi-layer adsorption. (chromatography-online.org)
- New adsorption operations of high-performance ion--pair chromatography have been investigated, using certain types of pharmacologically active nitrogen-bridged compounds. (elsevier.com)
Silica gel adsorbent1
- Our manufactured silica gel adsorbent is available in variety of grades and pore sizes at the most competitive prices. (column-chromatography.com)
Pore7
- By comparison the SOLAS™ MonoDense™ particles are monodense and contain a homogeneous pore structure which allows for the efficient transfer of analytes into and out of the silica pore structure, thereby leading to more efficient and effective chromatography. (glantreo.com)
- The degree of retardation (or the extent of the time taken by the molecule to reach the bottom) is proportional to the time it spends inside the pores of the gel which is a function of the molecules pore size and the molecules size. (microscopiaiwm.com)
- The material of the gel should have a wide variety of particle and pore sizes. (microscopiaiwm.com)
- Silica gel chromatography packing is made of monodisperse porous silica microspheres with highly uniform particle size, perfect spherical morphology and optimized pore structure. (epruibiotech.com)
- Our full range of standardized silica gels are available as granular (premium, standard, and economy grade) and spherical shapes with pore diameters ranging from 60Å to 2000Å, particle sizes from 3μm to 500μm, and bonded phases from C18 to SAX. (sorbtech.com)
- The chromatography column is packed with fine, porous beads which are composed of dextran polymers (Sephadex), agarose (Sepharose), or polyacrylamide (Sephacryl or BioGel P). The pore sizes of these beads are used to estimate the dimensions of macromolecules. (wikipedia.org)
- however, it was not until 1964, when J. C. Moore of the Dow Chemical Company published his work on the preparation of gel permeation chromatography (GPC) columns based on cross-linked polystyrene with controlled pore size, that a rapid increase of research activity in this field began. (wikipedia.org)
Compounds3
- Column chromatography is used for separating and purifying the liquids and solid compounds. (column-chromatography.com)
- Sorbead India offers quality silica gel for separating and purifying the mixed compounds using the column chromatography. (column-chromatography.com)
- Silica gel 230-400 and 400-800 mesh sizes are used in process chromatography to get more purity of active compounds. (sorbeadindia.com)
Fractionation2
- subu1iformis, was pure and homogenic after ethanol fractionation and purification by gel permeation chromatography (Fig. (thefreedictionary.com)
- other gels with size fractionation properties include agarose and polyacrylamide. (wikipedia.org)
Amount of silica gel1
- The different size and amount of silica gel is used based on the type of method and the other requirements. (column-chromatography.com)
Mesh3
- The size of the silica gel products varies in the mesh sizes of Silica Gel 60-120 Mesh, Silica Gel 60-200 Mesh, Silica Gel 70-230 Mesh, Silica Gel 100-200 Mesh and many more. (apsense.com)
- Just select the most suitable mesh size of silica gel as per the particular application and get the desired outcome. (column-chromatography.com)
- The practical size use of silica gel is 70-230 mesh and 63-210 microns. (sorbeadindia.com)
Preparative chromatography1
- Our silica gels are ideal for preparative chromatography, from laboratory to pilot-plant processes and production scale. (silicycle.com)
Polymer7
- Silica gel allows a controlled fragmentation leading to form uniform polymer particles with narrow particle size distribution and high bulk density. (sigmaaldrich.com)
- Silica gel supported zirconocene dichloride/methylalumoxane catalysts for ethylene polymerization: Effects of heterogenation on activity, polymer microstructure and product morphology. (sigmaaldrich.com)
- Gel permeation chromatography (GPC) is one of the most powerful and versatile analytical techniques available for understanding and predicting polymer performance. (reportsnreports.com)
- Founded in 1984 and headquartered in Houston, Texas, Viscotek offers systems for gel permeation chromatography , flow injection polymer analysis and dilute solution viscosity. (thefreedictionary.com)
- Accurate and repeatable polymer molecular weight information 5X to 20X faster ✓ One system for multiple analysese means more lab space and less capital spend ✓ Gather greater details about polymer samples using comprehensive 2D Chromatography. (chemeurope.com)
- GPC is a type of Size exclusion chromatography and is routinely used in polymer science for the determination of Mw and Mn, that is the weight and number average molecular weight of the polymer chains. (warwick.ac.uk)
- Gel Permeation Chromatography (GPC) for measurement of polymer molecular weight, size and structure. (malvernpanalytical.com)
Agarose2
- This protein was expressed in Escherichia coli and purified on a Ni 2+ -NTA-agarose column (Qiagen, Chatsworth, CA), followed by proteolysis with GST-TEV and subsequent collection of the flow-through from two sequential chromatography steps, the first over a Ni 2+ -NTA-agarose column and the second over a glutathione-agarose column. (pnas.org)
- Some examples of the types of gels used in gel permeation chromatography include sephadex, polyacrylamide, agarose, styragel etc. (microscopiaiwm.com)
Elution4
- In order to demonstrate the effectiveness of a silica gel column in ion-exclusion chromatography, the elution behavior of aliphatic carboxylic acids was investigated on an unmodified silica gel column having cation-exchange characteristics. (edu.au)
- Application of these different techniques and combination with the elution pattern of the gel permeation material revealed some valuable structural information. (ajevonline.org)
- Gel Permeation Chromatography (GPC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel. (malvernpanalytical.com)
- We extracted protein isoforms from polyacrylamide gels by passive elution using SDS, followed by nanoscale hydrophilic phase chromatography for SDS removal. (mcponline.org)
Column16
- This form of chromatography works by air pressure driving the mixture, which is to be separated down the vertical glass column. (buzzfeed.com)
- Lesser Silica gel and using Air or Nitrogen to drive the solvent gradient down the column. (apsense.com)
- Therefore, when the product is needed for separating the compound for any further analysis, it will be quite useful for processing the column chromatography. (apsense.com)
- The flash column chromatography has many different levels so that there are many pigment bands and absorption particles created for chromatography. (apsense.com)
- For column chromatography. (fishersci.com)
- The term gel permeation chromatography can be traced back to J.C. Moore of the Dow Chemical Company who investigated the technique in 1964 and the proprietary column technology was licensed to Waters Corporation, who subsequently commercialized this technology in 1964. (wikipedia.org)
- Column set: Jordi Gel fluorinated DVB columns (1-100K, 2-10K & 1-500Å). (uconn.edu)
- More material is removed from the gel during column recycling, and carry over of material from one cycle to the next is substantially reduced. (biopharminternational.com)
- Silica gel column chromatography depends on the activity of the silica gel as an adsorbent and polarity of the mixture. (column-chromatography.com)
- Prior to use, the gels used in the column must be converted to their swollen form by either soaking them in water or using a weak salt solution. (microscopiaiwm.com)
- The gel beds, in their swollen form, are mounted or supported on a column on a glass wool plug or nylon net and the previously swollen gel is added in the form of slurry. (microscopiaiwm.com)
- The monodisperse spherical porous silica gel has distinct advantages such as highly uniform particle size, perfect spherical morphology, outstanding mechanical strength, easy column packing, high column efficiency, good resolution, low back pressure and exceptional batch to batch reproducibility. (epruibiotech.com)
- Dynamic Adsorbents, Inc. distributes high quality silica gels for column chromatography and/or TLC with the lowest everyday price to many research institutions, pharmaceutical companies, and organic synthesis labs. (dynamicadsorbents.com)
- Silica gel is an ideal media for column chromatography for several different reasons. (dynamicadsorbents.com)
- Our recycling program will allow you to consolidate your LC column recycling and not to worry about what to do with old chromatography columns. (helixchrom.com)
- Aug 20, 1975 · The gel column chromatographic behaviour of 23 steroid oestrogens in the system developed for these investigations and tile application of this method for the determination of radio- chemical purity is described in this paper. (kelloggchurch.org)
Chromatographic8
- The adsorbents Spherosil 10, Silarex II and Partisil 10 were used to demonstrate the basic chromatographic differences between the three groups of silica gels. (chromatography-online.org)
- Gel permeation chromatography is a chromatographic procedure that separates molecules based on their molecular size. (microscopiaiwm.com)
- Dynamic Adsorbents' silica gels are carefully manufactured and quality assured to provide the ideal laboratory and pilot process chromatographic material. (dynamicadsorbents.com)
- Preparative gel chromatography on Sephadex LH-20 Chromatographic methods Hardcover - January 1, 1995 by Hans Henke Author › Visit Amazon's Hans Henke Page. (kelloggchurch.org)
- Dec 01, 1995 · Amazon.in - Buy Preparative Gel Chromatography on Sephadex LH-20 Chromatographic Methods book online at best prices in India on Amazon.in. (kelloggchurch.org)
- Read Preparative Gel Chromatography on Sephadex LH-20 Chromatographic Methods book reviews & author details and more at Amazon.in. (kelloggchurch.org)
- Sorbent Technologies™ offers a complete selection of silica gel products designed to perform simple to complex analytical and preparative chromatographic techniques for laboratory, pilot, and industrial process applications. (sorbtech.com)
- Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. (wikipedia.org)
Liquid3
- The experimental design is not much different from other techniques of liquid chromatography. (wikipedia.org)
- Conventional capillary liquid chromatography/mass spectrometry (LC/MS) typically employs low microl/min flow rates with gas/liquid sheath to enhance spray stability. (nih.gov)
- Journal of Liquid Chromatography , 8 (6), 1093-1107. (elsevier.com)
Methods4
- The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. (separationsnow.com)
- Ho K.K.Y., Valiontis E., Waters M.J. and Rajkovic I.A. (1993) Regulation of growth hormone binding protein in man: Comparison of gel chromatography and immunoprecipitation methods. (edu.au)
- Several techniques have been used to encapsulate PCMs such as in-situ polymerization which includes miniemulsion polymerization, complex coacervation, sol-gel methods, and solvent extraction/evaporation method, among others. (scielo.br)
- High resolution gel doc / imaging system detection methods include CCD / digital cameras, chemiluminescence, fluorescence imaging, small animal in vivo imagers, radioisotope and phosphor imaging. (selectscience.net)
Solvent1
- These effects were eliminated when LiCl was added to the dimethylformamide used as a solvent for lignin chromatography on Octylsepharose CL-4B and Sephadex LH-60 gels. (umn.edu)
Purification1
- Patient serum (30 [micro]L) was injected into a Waters 650E advanced protein purification system (Millipore) and subjected to high-pressure gel permeation chromatography (HPGPC). (thefreedictionary.com)
Adsorbents1
- In process chromatography use stationery phase adsorbents like silica gel and activated alumina are used in bulk quantity. (sorbeadindia.com)
Analytes2
- Crafted for scientists, the trainings are focused on gel permeation chromatography, which is a type of size-exclusion chromatography that separates analytes on the basis of size, typically in organic solvents. (photonics.com)
- Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size, typically in organic solvents. (wikipedia.org)
20161
- 40th International Symposium on Capillary Chromatography and 12th GC×GC Symposium , May 2016, Riva del Garda, Italy. (archives-ouvertes.fr)
Gravity2
- They include analytical, preparative, process and gravity chromatography. (column-chromatography.com)
- Gravity chromatography is a manual process of require continuously observed. (sorbeadindia.com)
Exclusion9
- Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. (separationsnow.com)
- What is size-exclusion chromatography? (shimadzu.com.au)
- GPC) - size-exclusion chromatography (SEC) carried out with organic mobile phases. (speciation.net)
- Therefore, the exclusion limit of a gel can be defined as the molecular weight of the smallest molecule which is not capable of entering the pores. (microscopiaiwm.com)
- At Custom Writing Bay, our professional writers are familiar with the principles and procedures that are involved in size exclusion chromatography. (customwritingbay.com)
- Size exclusion chromatography columns and resins (SEC) 7,5mm ID x600mm columns. (biokal.com)
- Gel permeation chromatography (GPC)/ Size exclusion chromatography (SEC) is a widely used technique for measuring the molecular weight and molecular weight distribution of natural and synthetic polymers. (itn-snal.net)
- Another use of size exclusion chromatography is to examine the stability and characteristics of natural organic matter in water. (wikipedia.org)
- Even though, size exclusion chromatography is widely utilized to study natural organic material, there are limitations. (wikipedia.org)
Columns6
- The high surface area of the coating, characteristic of materials fabricated by the sol-gel process, resulted in capillary columns with a stronger retention than those prepared by a conventional procedure. (springer.com)
- Gel permeation chromatography is conducted almost exclusively in chromatography columns. (wikipedia.org)
- DAI's bulk silica gels are used for low pressure and high pressure columns. (dynamicadsorbents.com)
- Mitsubishi Chemical Corporation serves the chromatography market since many years with MCI-Gel® Polymeric Columns and Resins. (biokal.com)
- Hydrophobic interaction chromatography columns and resins (HIC) with Phenyl, Butyl, or Ether as functional group. (biokal.com)
- MCI-Gel ® offers a range of analytical, preparative and production scale polymeric resins and columns for reversed phase chromatography. (biokal.com)
Size4
- Silica gel is one of the best for giving the result for removing the impurities from the micron size of 40. (apsense.com)
- To calculate the market size, the report considers the revenue generated from sales of gel permeation chromatography devices across the globe. (marketresearch.com)
- The greater is the porosity of the gel beads, more will be the time taken for them to attain equilibrium and reach their maximum size. (microscopiaiwm.com)
- The particle size, morphology, molecular weight, and thermal performance of nanoparticles containing the phase change material (PCM) were measured by dynamic light scattering, transmission electron microscopy, gel permeation chromatography, and differential scanning calorimetry, respectively. (scielo.br)
Polyolefins1
- High Temperature Gel Permeation Chromatography systems are especially useful for the analysis of Polyolefins, such as polyethylene and polypropylene, which represent the majority of industrial polymers. (jordilabs.com)
Separations1
- This type of chromatography is ideal for separations that need to take place in a fast paced manner. (buzzfeed.com)
Sephadex5
- Molecular weights obtained from these experiments were correlated with results from chromatography of lignin on Sephadex G-75 with 0.1N NaOH eluant and from those obtained using an analytical ultracentrifuge. (umn.edu)
- Jan 31, 1996 · Preparative Gel Chromatography on Sephadex Lh-20 by Hans Henke, 9783778524282, available at Book Depository with free delivery worldwide. (kelloggchurch.org)
- Preparative gel chromatography on Sephadex LH-20. (kelloggchurch.org)
- Preparative Gel Chromatography on Sephadex LH-20 by Hans Henke, Henke, Translated by Anthony J. Rackstraw, With 535 Figures, Hans 71 Tables Published December 23, 1998 by John Wiley & Sons Inc. Written in English. (kelloggchurch.org)
- Preparative gel chromatography on sephadex LH-20: with 71 tables. (kelloggchurch.org)
Quality silica2
- It is required to use quality silica gel in order to achieve excellent results. (column-chromatography.com)
- As with alumina, independent tests have shown that DAI produces the highest quality silica gel on the market today. (dynamicadsorbents.com)
Optimize1
- Focus on the latest chromatography technologies such as preparative and process chromatography to optimize the current and standard opportunities to optimize chromatography process in proper way. (sorbeadindia.com)
Porous1
- If porous glass granules are used, the gel beads need not be hydrated at all. (microscopiaiwm.com)
Molecular weight3
- n]) molecular weight and molecular weight distribution of the AA-copolymers were determined through gel permeation chromatography (GPC). (thefreedictionary.com)
- Gel permeation chromatography (GPC) can be used to assess the molecular weight of pharmaceuticals. (news-medical.net)
- Gel Permeation Chromatography (GPC) is a technique for measuring molecular weight. (news-medical.net)