A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The sum of the weight of all the atoms in a molecule.
Used as a support for ion-exchange chromatography.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
A cellulose of varied carboxyl content retaining the fibrous structure. It is commonly used as a local hemostatic and as a matrix for normal blood coagulation.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Cellulose derivative used in chromatography, as ion-exchange material, and for various industrial applications.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A species of acetate-oxidizing bacteria, formerly known as Acetobacter xylinum.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Chromatographic techniques in which the mobile phase is a liquid.
Electrophoresis in which cellulose acetate is the diffusion medium.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
High molecular weight, insoluble polymers which contain functional groups that are capable of undergoing exchange reactions (ION EXCHANGE) with either cations or anions.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The rate dynamics in chemical or physical systems.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Components of the extracellular matrix consisting primarily of fibrillin. They are essential for the integrity of elastic fibers.
Polysaccharides consisting of xylose units.
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The process of cleaving a chemical compound by the addition of a molecule of water.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The fruiting 'heads' or 'caps' of FUNGI, which as a food item are familiarly known as MUSHROOMS, that contain the FUNGAL SPORES.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
A species of gram-negative bacteria of the family ACETOBACTERACEAE found in FLOWERS and FRUIT. Cells are ellipsoidal to rod-shaped and straight or slightly curved.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).
A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.
The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A series of steps taken in order to conduct research.
A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The chemical and physical integrity of a pharmaceutical product.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
A family of bacteria found in the mouth and intestinal and respiratory tracts of man and other animals as well as in the human female urogenital tract. Its organisms are also found in soil and on cereal grains.
Proteins found in any species of bacterium.
Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Established cell cultures that have the potential to propagate indefinitely.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Dried, ripe seeds of PLANTAGO PSYLLIUM; PLANTAGO INDICA; and PLANTAGO OVATA. Plantain seeds swell in water and are used as demulcents and bulk laxatives.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
An exocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages of 1,4-beta-D-glucans resulting in successive removal of GLUCOSE units.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Proteins prepared by recombinant DNA technology.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)
Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A genus of gram-positive bacteria in the family Lachnospiraceae that inhabits the RUMEN; LARGE INTESTINE; and CECUM of MAMMALS.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A genus of gram-negative, anaerobic bacteria in the family Fibrobacteraceae, isolated from the human GASTROINTESTINAL TRACT.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Salts that melt below 100 C. Their low VOLATILIZATION can be an advantage over volatile organic solvents.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A key intermediate in carbohydrate metabolism. Serves as a precursor of glycogen, can be metabolized into UDPgalactose and UDPglucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. Also serves as a precursor of sucrose lipopolysaccharides, and glycosphingolipids.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).
The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)
Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.
The characteristic 3-dimensional shape of a carbohydrate.
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
A TEXTILE fiber obtained from the pappus (outside the SEEDS) of cotton plant (GOSSYPIUM). Inhalation of cotton fiber dust over a prolonged period can result in BYSSINOSIS.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Transport proteins that carry specific substances in the blood or across cell membranes.
The process of breakdown of food for metabolism and use by the body.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.

3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement. (1/2418)

3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 is involved in the degradative pathway of 3-nitrophenol, in which it catalyzes the conversion of 3-hydroxylaminophenol to aminohydroquinone. To show that the reaction was really catalyzed by a single enzyme without the release of intermediates, the corresponding protein was purified to apparent homogeneity from an extract of cells grown on 3-nitrophenol as the nitrogen source and succinate as the carbon and energy source. 3-Hydroxylaminophenol mutase appears to be a relatively hydrophobic but soluble and colorless protein consisting of a single 62-kDa polypeptide. The pI was determined to be at pH 4.5. In a database search, the NH2-terminal amino acid sequence of the undigested protein and of two internal sequences of 3-hydroxylaminophenol mutase were found to be most similar to those of glutamine synthetases from different species. Hydroxylaminobenzene, 4-hydroxylaminotoluene, and 2-chloro-5-hydroxylaminophenol, but not 4-hydroxylaminobenzoate, can also serve as substrates for the enzyme. The enzyme requires no oxygen or added cofactors for its reaction, which suggests an enzymatic mechanism analogous to the acid-catalyzed Bamberger rearrangement.  (+info)

Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4. (2/2418)

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.  (+info)

Synthesis and degradation of 1-aminocyclopropane-1-carboxylic acid by Penicillium citrinum. (3/2418)

1-Aminocyclopropane-1-carboxylic acid (ACC), which is a precursor of ethylene in plants, has never been known to occur in microorganisms. We describe the synthesis of ACC by Penicillium citrinum, purification of ACC synthase [EC] and ACC deaminase [EC], and their properties. Analyses of P. citrinum culture showed occurrence of ACC in the culture broth and in the cell extract. ACC synthase was purified from cells grown in a medium containing 0.05% L-methionine and ACC deaminase was done from cells incubated in a medium containing 1% 2-aminoisobutyrate. The purified ACC synthase, with a specific activity of 327 milliunit/mg protein, showed a single band of M(r) 48,000 in SDS-polyacrylamide gel electrophoresis. The molecular mass of the native enzyme by gel filtration was 96,000 Da. The ACC synthase had the Km for S-adenosyl-L-methionine of 1.74 mM and kcat of 0.56 s-1 per monomer. The purified ACC deaminase, with a specific activity of 4.7 unit/mg protein, showed one band in SDS-polyacrylamide gel electrophoresis of M(r) 41,000. The molecular mass of the native ACC deaminase was 68,000 Da by gel filtration. The enzyme had a Km for ACC of 4.8 mM and kcat of 3.52 s-1. The presence of 7 mM Cu2+ in alkaline buffer solution was effective for increasing the stability of the ACC deaminase in the process of purification.  (+info)

Purification and characterization of phosphoglycerate mutase from methanol-grown Hyphomicrobium X and Pseudomonas AM1. (4/2418)

Phosphoglycerate mutase has been purified from methanol-grown Hyphomicrobium X and Pseudomonas AMI by acid precipitation, heat treatment, ammonium sulphate fractionation, Sephadex G-50 gel filtration and DEAE-cellulose column chromatography. The purification attained using the Hyphomicrobium X extract was 72-fold, and using the Pseudomonas AMI extract, 140-fold. The enzyme purity, as shown by analytical polyacrylamide gel electrophoresis, was 50% from Hyphomicrobium X and 40% from Pseudomonas AMI. The enzyme activity was associated with one band. The purified preparations did not contain detectable amounts of phosphoglycerate kinase, phosphopyruvate hydratase, phosphoglycerate dehydrogenase or glycerate kinase activity. The molecular weight of the enzymic preparation was 32000 +/- 3000. The enzyme from both organisms was stable at low temperatures and, in the presence of 2,3-diphosphoglyceric acid, could withstand exposure to high temperatures. The enzyme from Pseudomonas AMI has a broad pH optimum at 7-0 to 7-6 whilst the enzyme from Hyphomicrobium X has an optimal activity at pH 7-3. The cofactor 2,3-diphosphoglyceric acid was required for maximum enzyme activity and high concentrations of 2-phosphoglyceric acid were inhibitory. The Km values for the Hyphomicrobium X enzyme were: 3-phosphoglyceric acid, 6-0 X 10(-3) M: 2-phosphoglyceric acid, 6-9 X 10(-4) M; 2,3-diphosphoglyceric acid, 8-0 X 10(-6) M; and for the Pseudomonas AMI ENzyme: 3-4 X 10(-3) M, 3-7 X 10(-4) M and 10 X 10(-6) M respectively. The equilibrium constant for the reaction was 11-3 +/- 2-5 in the direction of 2-phosphoglyceric acid to 3-phosphoglyceric acid and 0-09 +/- 0-02 in the reverse direction. The standard free energy for the reaction proceeding from 2-phosphoglyceric acid to 3-phosphoglyceric acid was -5-84 kJ mol(-1) and in the reverse direction +5-81 kJ mol(-1).  (+info)

The complete amino acid sequence of dog beta2-microglobulin. (5/2418)

Dog beta2-microglobulin was purified from the urine of dogs with potassium dichromate induced tubular damage. It was purified by sequential use of anion exchange chromatography, gel filtration chromatography, and reversed-phase high performance liquid chromatography. Comparisons of the amino acid sequence of the dog protein with human, mouse, and rabbit beta2-microglobulin, indicated a high degree of similarity. The dog protein was very similar to human beta2-microglobulin in that it had a molecular weight of 11.8 kDa and contained two half-cystinyl residues. Dog and human beta2-microglobulin were demonstrably different at 24 of the 99 positions compared. The data supported the conclusion that the purified protein was dog beta2-microglobulin and that all four proteins from dog, human, mouse, and rabbit were closely related.  (+info)

Polar lipids of four Listeria species containing L-lysylcardiolipin, a novel lipid structure, and other unique phospholipids. (6/2418)

The membrane lipids of Listeria innocua, Listeria monocytogenes, Listeria seeligeri and Listeria welshimeri were fractionated on DEAE-cellulose and purified by chromatography on silica gel and/or preparative TLC. The lipid structures were elucidated by chemical and chromatographic means. The polar lipid composition of the four listeria species was similar. Phospholipids predominated. They consisted of phosphatidylglycerol, L-lysylphosphatidylglycerol, cardiolipin [bis(phosphatidyl)glycerol] and L-lysylcardiolipin. A phospholipid more polar than cardiolipin, possibly two L-lysyl derivatives of it, sn-glycero-1-phosphoglycolipid, its D-alanyl derivative, and polyprenol phosphate were also detected. Towards the end of exponential growth, the relative amounts of cardiolipin and L-lysylcardiolipin increased, approaching 47-78% lipid phosphorus with a ratio of L-lysylcardiolipin to cardiolipin of 0.25-1.6. As shown by fast atom bombardment-mass spectrometry, cardiolipin and L-lysylcardiolipin consisted of five molecular species due to various fatty acid combinations. L-lysylcardiolipin has so far not been found in nature. It belongs to the recently discovered class of substituted cardiolipins. Its occurrence in the four listeria species tested shows that it is a characteristic lipid component of the L. monocytogenes line of descent. Further studies on the lipid pattern of members of the other descent line are required to decide whether lysylcardiolipin can serve as a genus-specific chemotaxonomic marker for listeriae.  (+info)

Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans. (7/2418)

Substitution of the asparagine-linked GlcNAc by alpha1,3-linked fucose is a widespread feature of plant as well as of insect glycoproteins, which renders the N-glycan immunogenic. We have purified from mung bean seedlings the GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase (core alpha1,3-fucosyltransferase) that is responsible for the synthesis of this linkage. The major isoform had an apparent mass of 54 kDa and isoelectric points ranging from 6. 8 to 8.2. From that protein, four tryptic peptides were isolated and sequenced. Based on an approach involving reverse transcriptase-polymerase chain reaction with degenerate primers and rapid amplification of cDNA ends, core alpha1,3-fucosyltransferase cDNA was cloned from mung bean mRNA. The 2200-base pair cDNA contained an open reading frame of 1530 base pairs that encoded a 510-amino acid protein with a predicted molecular mass of 56.8 kDa. Analysis of cDNA derived from genomic DNA revealed the presence of three introns within the open reading frame. Remarkably, from the four exons, only exon II exhibited significant homology to animal and bacterial alpha1,3/4-fucosyltransferases which, though, are responsible for the biosynthesis of Lewis determinants. The recombinant fucosyltransferase was expressed in Sf21 insect cells using a baculovirus vector. The enzyme acted on glycopeptides having the glycan structures GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1- 6)Manbeta1-4GlcNAcbet a1-4GlcNAcbeta1-Asn, GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1- 6)Manbeta1-4GlcNAcbet a1-4(Fucalpha1-6)GlcNAcbeta1-Asn, and GlcNAcbeta1-2Manalpha1-3[Manalpha1-3(Manalpha1-6 )Manalpha1-6]Manbeta1 -4GlcNAcbeta1-4GlcNAcbeta1-Asn but not on, e.g. N-acetyllactosamine. The structure of the core alpha1,3-fucosylated product was verified by high performance liquid chromatography of the pyridylaminated glycan and by its insensitivity to N-glycosidase F as revealed by matrix-assisted laser desorption/ionization time of flight mass spectrometry.  (+info)

Steroid monooxygenase of Rhodococcus rhodochrous: sequencing of the genomic DNA, and hyperexpression, purification, and characterization of the recombinant enzyme. (8/2418)

Steroid monooxygenase of Rhodococcus rhodochrous is a Baeyer-Villigerase catalyzing the insertion of an oxygen atom between the C(17)- and C(20)-carbons of progesterone to produce testosterone acetate. The 5.1-kbp-long BamHI DNA fragment containing the steroid monooxygenase gene, smo, was cloned from the chromosomal DNA and sequenced. The smo gene is 1,650 nucleotides long, starts with a TTG codon, and ends with a TGA codon. The deduced amino acid sequence indicates that the enzyme protein consist of 549 amino acid residues with a molecular mass of 60,133. Thus, the molecular mass of the holoenzyme is 60,919. The amino acid sequence is highly homologous (41.2% identity) to that of cyclohexanone monooxygenase of Acinetobacter sp. In the upstream of the smo gene, the genes of heat shock proteins, dnaK, grpE, and dnaJ, located on the complementary strand, and the DNA-inserts of pSMO and pD1, which contains the ksdD gene, were joined at the BamHI site of the dnaJ gene. The smo gene was modified at the initiation codon to ATG and ligated with an expression vector to construct a plasmid, pSMO-EX, and introduced into Escherichia coli cells. The transformed cells hyperexpressed the steroid monooxygenase as an active and soluble protein at more than 40 times the level in R. rhodochrous cells. Purification of the recombinant monooxygenase from the E. coli cells by simplified procedures yielded about 2.3 mg of enzyme protein/g wet cells. The purified recombinant steroid monooxygenase exhibited indistinguishable molecular and catalytic properties from those of the R. rhodochrous enzyme.  (+info)

The effect of low dose heparin therapy on fibrinogen survival in patients with cirrhosis was studied in six patients. Survival of 1-125 radiolabeled fibrinogen was measured using both autologous and homologous material. Average fibrinogen half-life before heparin therapy was 52 hours and after 3000 units of intravenous heparin every 6 hours was 101.8 hours. Median survival before heparin therapy was 56 hours and after therapy was 91 hours. In every instance fibrinogen survival was improved by heparin administration. These data indicate that low dose heparin improves fibrinogen survival in cirrhosis and suggest that disseminated intravascular coagulation is a primary process in the defibrination syndrome associated with cirrhosis. ...
15-3 Cap Structure Purified cap by DEAE chromatography; digested with enzymes to figure structure  -phosphate of NTP remains only in first nucleotide in RNA -Cap is 5-terminus of RNA -Cap is m 7 G, 7- methylguanosine, -Linkage is triphosphate -Charge on cap area is ~ -5 Fig. 1 vaccinia virus 3H methyl from S-AdoMet; 32P-GTP label RNA); KOH to digest RNA Fig. 2 shows me7-G
Methods:Purification included: extraction of the enzyme, the precipitation of the enzyme by ammonium sulphate, dialysis, ionic exchange chromatography by using DEAE-Cellulose (Diethylaminoethyl-Cellulose), and gel filtration by using Sephacryl S-200. Equal amounts of purified lipase solution were mixed with PBS (Phosphate buffer sodium) solutions of different pH (4,5,... until 10) and incubated in a water bath at 37 oC for 30 minutes, then the lipase activity was estimated. The purified lipase was incubated at different degrees of temperature (5, 15, ...until 85 oC) for 30 minutes. The molecular weight was determined by gel filtration chromatography ...
Chondrex provides various types and species of collagen for general use such as for immunizing animals, tissue engineering, cell culture, and antibody assays. These collagen products are highly purified by repetitive salt precipitation and DEAE-cellulose chromatography to remove contaminants such as proteoglycans and pepsin, which are more antigenic than collagen.. Immunization Grade Type I Collagen. ...
Brisas del Descanso is a neighborhood in Cauca. Brisas del Descanso is situated nearby to Padilla. Brisas del Descanso from Mapcarta, the open map.
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Merriam, E. Virginia and Dumas, Lawrence B. and Sinsheimer, Robert L. (1970) A method for analysis of DNA by annealing on a DNA cellulose column. Analytical Biochemistry, 36 (2). pp. 389-395. ISSN 0003-2697. https://resolver.caltech.edu/CaltechAUTHORS:20160728-084236243 Full text is not posted in this repository. Consult Related URLs below.. Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160728-084236243 ...
TY - JOUR. T1 - Heterogeneity of rat Liver SulfotransferaseS. AU - Matsui, Michio. AU - Nagai, Fusako. N1 - Copyright: Copyright 2016 Elsevier B.V., All rights reserved.. PY - 1985. Y1 - 1985. N2 - Sulfotransferases (STs)active on androsterone (AD), cortisol (CS) and 4-nitrophenol (NP) were separated by diethylaminoethyl-cellulose chromatography from cytosolic fractions of female rat liver and were divided into five ST fractions (peaks I-V) with different activities toward three substrates. The precipitates obtained in the 68% of saturation of ammonium sulfate were passed through a Sephadex G-100 column and purified by agarose-hexane adenosine 3\5-bisphosphate affinity chromatography. AD-ST isoenzyme (peak I) was purified 85-fold, had low CS-ST activity, was devoid of NP-ST activity and appeared to correspond to hydroxysteroid ST 1. Peaks II and V appeared to consist mainly of hydroxysteroid ST and aryl ST, respectively.. AB - Sulfotransferases (STs)active on androsterone (AD), cortisol (CS) ...
Soluble extracts prepared from bovine thymus contain an angiotensin-I-phosphorylating activity that is activated several-fold by high concentrations of NaCl. Fractionation of this protein-tyrosine kinase activity by chromatography on DEAE-cellulose yields a major diffuse peak of activity. The enzymes responsible for this activity are found at much higher levels in extracts from bovine thymus than from bovine spleen. The peak of activity from the DEAE-cellulose column can be further separated into two major peaks by chromatography on heparin-agarose. The second peak to elute from the heparin-agarose column was previously purified through several chromatographic steps to yield a 40 kDa protein-tyrosine kinase (p40). We have now partially purified the early-eluting peak of kinase activity by chromatography on columns of butyl-agarose, protamine-agarose and Sephacryl S200. The enzyme was identified following covalent modification with 5′-fluorosulphonylbenzoyladenosine (FSBA) by reactivity with ...
1. Monoamine oxidase from rat and human liver was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. 2. The enzyme activity was extracted from mitochondrial preparations by Triton X-100. The enzyme was purified by (NH4)2SO4 fractionation followed by chromatography on DEAE-cellulose, Sepharose 6B, spheroidal hydroxyapatite, and finally chromatography on diazo-coupled tyramine-Sepharose. 3. Distinct differences occur in the chromatographic behaviour of the two enzymes on both DEAE-cellulose and spheroidal hydroxyapatite. 4. It is unlikely that the purification of the enzymes on tyramine-Sepharose is due to affinity chromatography and reasons for this are discussed. 5. The purified enzymes did not oxidize-5-hydroxytryptamine and the relative activities of the enzymes with benzylamine were increased approx. 1.25-fold compared with the enzyme activities of mitochondrial preparations. 6. Immunotitration of enzyme activity in ...
Mouse monoclonal anti-human Transferrin antibody DEAE chromatography purified Isotype IgG1 Source Mouse ascites Immunogen Recombinant human Transferrin antigen purified from cellculture supernatant Purity |95% by HPLC and SDS-PAGE Application for CEA quantitativeassays by EIA Storage
Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structure of LEP-2a was characterized by methylation analysis, FT-IR analysis, and NMR analysis. Results indicated that LEP-2a was a galactomannan with a backbone, composed of 1,2-linked-α-D-Manp, 1,2,6-linked-α-D-Manp, 1,3,4-linked-α-D-Manp, and 1,3-linked-β-D-Galp, which was substituted at O-2, O-3, O-4, and O-6 by branches ...
TSKgel® DNA-NPR Guard Column phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 5 μm particle size; Synonym: TSKgel® DEAE-NPR HPLC Column; find null-817088 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
65647-14-5 - DUKOQRXNBYNJED-UHFFFAOYSA-N - 5H-1,4-Benzodiazepin-5-one, 1,2,3,4-tetrahydro-4-(2-diethylaminoethyl)-1-phenyl- - Similar structures search, synonyms, formulas, resource links, and other chemical information.
14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 200 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis by Moore and Perez (1967). Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. There are seven genes that encode seven distinct 14-3-3 proteins in most mammals (See Human genes below) and 13-15 genes in many higher plants, though typically in fungi they are present only in pairs. Protists have at least one. ...
Guanine deaminase Proteins available through Novus Biologicals. Browse our Guanine deaminase Protein catalog backed by our Guarantee+.
Hordeum vulgare CAB-3 protein: from barley; chlorophyl a-b-binding protein of the light-harvesting complex II; amino acid sequence given in first source
Werner, C.G., Godrey, V., Arnold, R.R., Featherstone, G.L., Bender, D., Schlossmann, Jens, Schiemann, M., Hofmann, F. and Pryzwansky, K.B. (2005) Neutrophil dysfunction in guanosine 3,5-cyclic monophosphate-dependent protein kinase I-deficient mice. Journal of Immunology 175, pp. 1919-1929 ...
1-(2-Diethylaminoethyl)-2-methyl-3-(2-methylphenyl)-2H-quinazolin-4-one;oxalic acid/ACM34963489 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Whatman 3MM Chr paper is the worlds most widely used blotting paper. This acceptance and usage reflect the high quality, purity, and consistency that are relied upon by researchers doing Southern, Northern, and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) is used extensively in electrophoresis for lifting of sequencing gels.. Read more ...
The immunoglobulin having reaginic activity was purified from sera of ragweed sensitive patients by salt precipitation, diethylaminoethyl (DEAE)-cellulose column chromatography, gel filtration and DEAE-Sephadex column chromatography. The γG- and γA-globulins remaining in the purified samples were absorbed with rabbit anti-γG- and anti-γA-antibodies which had been precipitated with goat antibody against rabbit γ-globulin. On a weight basis, reaginic activity of the final preparation was about 1000 times more active than the original sera and the most active fraction gave positive P-K reactions at a dilution of 1:80,000. Human serum proteins detected in the preparation were γE- and γD-globulins. The antibody against antigen E was detected in γE-globulin but not in other immunoglobulins. It was also found that the reaginic activity and γE-antibody in the preparations were precipitated by anti-γE-antibody. The results indicate that reaginic activity is associated with γE-globulin. ...
casSAR Dugability of Q9Y2T3 | GDA | Guanine deaminase - Also known as GUAD_HUMAN, GDA, KIAA1258. Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Homodimer.
GE Healthcare HiTrap™ DEAE Sepharose Fast Flow IEX Columns Prepacked w/DEAE Sepharose Fast Flow, weak anion; 5 x 1mL GE Healthcare HiTrap™ DEAE...
Application NumberNDA021323Brand NameLEXAPROGeneric NameESCITALOPRAM OXALATEManufacturersAllergan, Inc.product_ndc0456-2005, 0456-2010, 0456-2020, 0456-2101Product TypeHUMAN PRESCRIPTION DRUGRouteORALActive IngredientsESCITALOPRAM OXALATERXCUI349332, 351249, 351250, 351285, 352272, 352273, 404408, 404420spl_idf7659260-4527-4446-9aa5-15a2895c5b0espl_set_id13bb8267-1cab-43e5-acae-55a4d957630aPackage NDC 0456-2005-01, 0456-2010-01, 0456-2010-11, 0456-2010-63, 0456-2020-01, 0456-2020 ...
Yes there was a change. AFAIK it is related to multi selection behaviour. I guess you configured the selection model to not allow multiple selection. Before column selection did not respect that configuration. So the behaviour now should be correct while before it was wrong ...
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Cell extracts, in the presence of dimethylsulfoxide (DMSO), catalyzed the conversion of pyrogallol to phloroglucinol with dimethylsulfide as a product. The isomerization reaction would also proceed if 1,2,3,5-benzenetetrol was present rather than DMSO. To quantitate this activity, an assay was developed that followed the disappearance of 1,2,4-benzenetriol colorimetrically after incubation with sodium molybdate at neutral pH. The products of this reaction are resorcinol and 1,6-dihydroxyquinone. The enzyme(s) catalyzing this reaction was purified 5-fold from cells grown on gallate. The purification procedure involved fractionation with 40% acetone, precipitation with ammonium sulfate, DEAE-cellulose chromatography, concentration by ultrafiltration (molecular weight retention above 100,000), and hydroxylapatite chromatography. This preparation had a specific activity of 14.7 umol/min/mg protein and a pH optimum about 7.5. Activity was strongly inhibited by p-chloromercuribenzoate. The mechanism ...
Abstract: In presence of adenosine (10(-7)-10(-6) M) content of nuclear 3H-hydrocortisone-receptor complexes was increased in rat thymus lymphocytes, while amount of these complexes was decreased in cytosol of these cells. DEAE-cellulose chromatography of the 3H-hydrocortisone-receptor complexes demonstrated that adenosine 1.10(-6) M altered the ratio between active and non-active forms of the hormone-receptor complex. Adenosine appears to regulate transformation and translocation of the glucocorticoid-receptor complexes into the cell-target nuclei cAMP-dependent apparatus ...
CAB-171-15NF can be used as coating material as the semi-permeable membrane for osmotic drug delivery. It has the lowest utyryl conent in the Cellulose Acetate Butyrate series.
Whatman™ Grade 1 Chr Cellulose Chromatography Paper Type: roll; L x W: 328 ft. x 0.39 in. (100m x 10mm) Whatman™ Grade 1 Chr Cellulose...
소각 X 선 산란 (SAXS)에 의한 단백질의 용액 구조의 결정은 단 분산 샘플을 필요로한다. 여기에서는, 샘플 준비 및 데이터 수집 간의 최소 지연을 보장하기 위해 두 가지 가능성을 제시 : 온라인 크기 배제 크로마토 그래피 (SEC) 및 ...
Bestemmelsen af ​​opløsningen struktur af et protein ved lille vinkel røntgenspredning (SAXS) kræver monodisperse prøver. Her...
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Rats were immunized with crude Ascaris extracts together with Bordetella pertussis organisms and the homocytotropic antibody titers were estimated by homologous PCA. Rats injected with 1 mg of crude extract produced homocytotropic antibodies giving and average PCA titer of 1:80. A second injection of 100 µg of the extract given 6 days later, without B. pertussis vaccine, caused a considerable increase in the PCA titer. The crude extract was fractionated by DEAE-cellulose chromatography and the fractions were tested in P-K type reactions. The homocytotropic antibodies produced in rats immunized with the crude extract demonstrated a tendency to react with the fractions displaying a negative charge at pH 8.. The capacity of rats to produce Hc antibodies to part of the antigenic components present in the crude extract but not against others was demonstrated when rats were immunized separately with isolated fractions.. ...
This seems like it should be easy enough, but I cant seem to find an easy answer. I have information on column A and column B but I want the cells in column B to be highlighted if it doesnt match column A. I could do it one at a time but with a few hundred lines, it would take a while ...
Click on column heading text to sort by a column. Initial column will be sorted in descending order, click again to get ascending order. The type of data in the cell is determined automatically: ...
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We investigated the expression of the alpha- and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase in the BV-2 microglial cell line under different culture conditions. Beta-N-acetylhexosaminidase from BV-2 microglia cells was separated into its constituent isoenzymes on diethylaminoethyl (DEAE) cellulose, and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine-6-sulphate substrates. Forms corresponding to the mouse isoenzymes A and B were present in the cells incubated in serum-supplemented medium as well as in serum-free medium. Lipopolysaccharide, a well-known activator of microglia in vitro, added to the BV-2 cells in serum-supplemented medium induced a decrease in the specific enzymatic activity determined with the 4-methylumbelliferyl-beta-N-acetylglucosamine substrate. Lipopolysaccharide had no effect on hexosaminidase isoenzyme pattern of BV-2 cells in serum-supplemented medium. The level of ...
Moen, Line Victoria; Ramberg, Håkon Andre; Zhao, Sen; Grytli, Helene Hartvedt; Sveen, Anita; Berge, Viktor; Skotheim, Rolf I.; Tasken, Kristin Austlid & Skålhegg, Bjørn Steen (2017). Observed correlation between the expression levels of catalytic subunit, C?2, of cyclic adenosine monophosphate-dependent protein kinase and prostate cancer aggressiveness. Urologic Oncology. ISSN 1078-1439. 35(3), s 111.e1- 111.e8 . doi: 10.1016/j.urolonc.2016.10.002 Vis sammendrag Background As an intracellular human pathogen, Mycobacterium tuberculosis (Mtb) is facing multiple stressful stimuli inside the macrophage and the granuloma. Understanding Mtb responses to stress is essential to identify new virulence factors and pathways that play a role in the survival of the tubercle bacillus. The main goal of this study was to map the regulatory networks of differentially expressed (DE) transcripts in Mtb upon various forms of genotoxic stress. We exposed Mtb cells to oxidative (H2O2 or paraquat), nitrosative ...
Eastman Cellulose Acetate Butyrate (CAB 381-0.1, Food Contact) is a cellulose ester with medium butyryl content and low viscosity. It was designed for use where low-application viscosities at relatively high solids levels are needed. It is soluble in a wide range of solvents and compatible with many other resins. When CAB-381-0.1, Food Contact is dissolved in appropriate solvents a clear, colorless solution is produced. CAB-381-0.1, Food Contact will also tolerate the use of solvent blends currently exempt from certain air pollution regulations. It is supplied as a dry, free-flowing powder.. Eastman CAB-381-0.1, Food Contact is based on cellulose, one of the most abundant natural renewable resources, from trees harvested from sustainably managed forests. The calculated approximate bio-content value of 41% for Eastman CAB-381-0.1, Food Contact was determined by using six bio-based carbon atoms per anhyroglucose unit divided by the total number of carbons per anhyroglucose unit. Although the value ...
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
Indulge your dog in flights of fancy with this bee and butterfly adorned harness. To add to the glamour, each of the individual embroidered bees and butterflies is accented with crystal. Like all of our designs, it is made from genuine upholstery ...
... it can be adsorbed on chromatography columns containing DEAE-cellulose as the adsorbent; it has a pH optimum of 4.5, lower than ...
DEAE can be used as a precursor for DEAE-cellulose resin, which is commonly used in ion exchange chromatography. DEAE can also ... "Experiments and model for the surface tension of DEAE-PZ and DEAE-MEA aqueous solutions". The Journal of Chemical ... Diethylethanolamine (DEAE) is a chemical compound with the molecular formula C6H15NO. It is used as a precursor in the ...
... which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. The enzyme-activity was inhibited ... 1990) with the help of membrane ultra filtration and C-75 gel chromatography. He purified enzyme with 70-fold increased ...
In bovine brain samples, 14-3-3 proteins were located in the 14th fraction eluting from a DEAE-cellulose column and in position ... 14-3-3 proteins were initially found in brain tissue in 1967 and purified using chromatography and gel electrophoresis. ...
... chromatography, ion exchange MeSH E05.196.181.400.383.349 - chromatography, deae-cellulose MeSH E05.196.181.400.454 - ... chromatography, liquid MeSH E05.196.181.400.170 - chromatography, affinity MeSH E05.196.181.400.250 - chromatography, gel MeSH ... chromatography, thin layer MeSH E05.196.181.400.555 - countercurrent distribution MeSH E05.196.181.500 - chromatography, ... E05.196.181.400.250.200 - chromatography, agarose MeSH E05.196.181.400.300 - chromatography, high pressure liquid MeSH E05.196. ...
DEAE is an anion exchange matrix that is produced from a positive side group of diethylaminoethyl bound to cellulose or ... This type of chromatography is further subdivided into cation exchange chromatography and anion-exchange chromatography. ... or in chromatography columns. Thin layer chromatography or column chromatography share similarities in that they both act ... Ion chromatography (or ion-exchange chromatography) separates ions and polar molecules based on their affinity to the ion ...
Alkaline phosphatase from E. coli can be purified using a DEAE-Cellulose matrix. A. phosphatase has a slight negative charge, ... Lectin affinity chromatography is a form of affinity chromatography where lectins are used to separate components within the ... Weak affinity chromatography (WAC) is an affinity chromatography technique for affinity screening in drug development. WAC is ... By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that ...
DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger. This exchange ... Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column ... and an efficient separation with DEAE-C chromatography requires a specific, narrow pH range. Cellulose, dextran, agarose, and ... DEAE-C beads have diethylaminoethyl chains covalently bound to oxygen atoms on the D-glucose subunits of cellulose. Size- ...
Its largest application is for the conversion of cellulose to cellulose acetate, which is a component of photographic film and ... doi:10.15227/orgsyn.005.0017 Taber, Douglass F., Column chromatography: Preparation of Acetyl Ferrocene, Department of ... DEA List II precursor, and restricted in many other countries. Acetic anhydride is an irritant and combustible liquid; it is ... when the demand for acetic anhydride increased due to the production of cellulose acetate. Due to its low cost, acetic ...
Industrial and analytical ion-exchange chromatography is another area to be mentioned. Ion-exchange chromatography is a ... CM (Carboxymethyl group, weak cation exchange) SP (sulphopropyl group, strong cation exchange) DEAE-Sepharose QFF Ion exchange ... "Synthesis of super hydrophilic cellulose-alpha zirconium phosphate ion exchange membrane via surface coating for the removal of ... Alkali anion exchange membrane Ion Ion chromatography Ion-exchange membranes Ion-exchange resin Desalination Reverse osmosis ...
Chromatography, DEAE-Cellulose * Chromatography, Gel * Chromatography, Paper * Clostridium / enzymology* * Electrophoresis * ...
... were partially separated from mosquito debris and microbial contaminants by passage of Anopheles material through a DEAE- ... cellulose column. In addition to eliminating most of the conta … ... Chromatography, DEAE-Cellulose * Malaria / etiology * Mice * Plasmodium / immunology * Plasmodium / isolation & purification* * ... partially separated from mosquito debris and microbial contaminants by passage of Anopheles material through a DEAE-cellulose ...
Anion exchange chromatography on DEAE -Cellulose. The supernatant was diluted 10x with distilled water and loaded on a DEAE- ... anion exchange chromatography on DEAE-Cellulose; filtration on Sephacryl S200; anion exchange chromatography on Q-Sepharose FF ... C) Chromatography of rTPL on Sephadex G100; the column was equilibrated in buffer B and the flow rate was 25 ml/h. The pooled ... A) Chromatography of rTPL on Sephacryl S200; the column (2.5 × 150 cm) was equilibrated with 25 mM Tris buffer, pH 8.2, ...
DEAE-cellulose chromatography showed increased ornithine-decarboxylase antizyme activity in liver microsomal fractions from ...
Anion exchange chromatography (DEAE-cellulose column) was performed to separate the crude polysaccharides obtained via water ... The aqueous extract of Tremella fuciformis was purified using a DEAE-52 cellulose anion exchange column and a Sepharose G-100 ... H2O and subjected to the DEAE-52 cellulose anion exchange column (2.6 × 35 cm; Whatman; GE Healthcare Life Sciences, Chalfont, ... A) DEAE-Sepharose fast flow chromatogram of the crude polysaccharides, which was eluted using double distilled H2O at a flow ...
DEAE cellulose chromatography, dialysis and followed by rechromatography on DEAE cellulose column as described in our earlier ...
was selected for enzyme purification by ammonium sulfate precipitation, DEAE-cellulose and CM-cellulose column chromatography, ... Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC) agar plate ... DEAE-cellulose column chromatography. 60 ml of the enzyme sample was applied to DEAE-cellulose (Diethylaminoethyl cellulose) ... CM-cellulose column chromatography. 55 ml of DEAE unbound solution was applied to CM-cellulose column which was equilibrated ...
Chromatography, DEAE-Cellulose Entry term(s). Chromatography, DEAE Cellulose DEAE Cellulose Chromatography DEAE-Cellulose ... Chromatographie sur DEAE-cellulose Entry term(s):. Chromatography, DEAE Cellulose. DEAE Cellulose Chromatography. DEAE- ... Chromatography, DEAE-Cellulose - Preferred Concept UI. M0004375. Scope note. A type of ion exchange chromatography using ... A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From ...
Chromatography, DEAE-Cellulose. en_US. dc.subject.mesh. Glycosaminoglycans --analysis. en_US. ...
... as above and subsequently by column chromatography on DEAE cellulose in a gradient of 0.02-0.8M triethylammonium bicarbonate. ... as judged both by migration on thin layer DEAE plates, in Homo V Jay, E. et al., Nucleic Acids Research, 1: 331-353 (1974), and ... further purification was performed by high pressure liquid chromatography, using a Waters SAX Radial-Pak catridge or by ...
... and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined ...
... and DEAE cellulose chromatography of T1 RNase digestion products of aminoacyl-tRNA. In all experiments, the preparations from ... seryl and alanyl oligonucleotides on DEAE cellulose chromatography, are shown. In all cases, the distribution patterns of the [ ... The separation patterns of arginyl-tRNA and lysyl-tRNA on MAK column chromatography, of threonyl-tRNA, seryl-tRNA and arginyl- ... compared using standard chromatographic techniques such as MAK column chromatography and Reverse phase type II chromatography ...
... calcium-activated proteinases has been purified from porcine skeletal muscle by using DEAE-cellulose column chromatography, ... calcium-activated proteinases has been purified from porcine skeletal muscle by using DEAE-cellulose column chromatography, ... calcium-activated proteinases has been purified from porcine skeletal muscle by using DEAE-cellulose column chromatography, ... calcium-activated proteinases has been purified from porcine skeletal muscle by using DEAE-cellulose column chromatography, ...
Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine. ...
The crude toxic extract was fractionated by silica gel, LH-20 Sephadex gel and DEAE-cellulose column chromatography, and a 27- ... The crude toxic extract was fractionated by silica gel, LH-20 Sephadex gel and DEAE-cellulose column chromatography, and a 27- ...
DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated ... Capillary electrophoresis, high‐performance liquid chromatography, and thin‐layer chromatography analyses of phenolic compounds ... cellulose; dose response; ethanol; fever; food industry; free radicals; functional foods; galactose; gel chromatography; ... high performance liquid chromatography; leaves; oils; phenolic acids; polyphenols; rapeseed; seeds; thin layer chromatography. ...
DEAE Cellulose use DEAE-Cellulose DEAE Cellulose Chromatography use Chromatography, DEAE-Cellulose ... DEAE Sephadex use DEAE-Dextran DEAE-Cellulose DEAE-Cellulose Chromatography use Chromatography, DEAE-Cellulose ...
DEAE-32) column. Results We show that creatine kinase activity is significantly inhibited by nicotine (44%), cotinine (39%) and ... Materials and methods Total creatine kinase activity is measured in sperm homogenates after chromatography on a ... Total creatine kinase activity is measured in sperm homogenates after chromatography on a diethylaminoethyl cellulose (DEAE-32 ...
In addition, two novel polysaccharides (LNP-1, LNP-2) were purified by DEAE Cellulose-52 and Sephadex G-150 chromatography. The ...
The proteins in both protein‐rich fractions appear to be the same as judged by chromatography and zone electrophoresis. ... composition.Proteins extracted from mature seeds of Arachis hypogaea appear heterogeneous by chromatography on DEAE-cellulose, ... composition.Proteins extracted from mature seeds of Arachis hypogaea appear heterogeneous by chromatography on DEAE-cellulose, ... Lipids extracted from the subcellular fractions were analyzed by thin-layer chromatography.…" ...
... the isolation of a tubulin-calmodulin kinase complex from rat brain cytosol by sequential chromatography on DEAE-cellulose, ... The kinase, purified from chelated brain cytosol by sequential chromatography on phosphocellulose, calmodulin-affinity resin, ... the isolation of a tubulin-calmodulin kinase complex from rat brain cytosol by sequential chromatography on DEAE-cellulose, ... The kinase, purified from chelated brain cytosol by sequential chromatography on phosphocellulose, calmodulin-affinity resin, ...
... partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, ... partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, ... partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, ... partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, ...
2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex, affinity ... ...
E134). The 2X elastase is chromatographed on DEAE-cellulose at pH 8.8 to remove anionic non-specific protein. The elastolytic ... Trace trypsin, and chymotrypsin contamination is removed by affinity chromatography.. PURITY.. The high purity elastase is ... homogenous on 15% polyacrylamide electrophoresis at pH 4.5 and on gel exclusion chromatography (G200) at pH 5.0 and 25°C.. FREE ... effluent is chromatographed at pH 5.0 on CM-cellulose recovering the highest specific activity by elution with salt gradient. ...
DEAE-cellulose chromatography and Sephadex-G-100 gel filtration. A solvent system was developed for thin layer chromatography ... The product was identified as cAMP by cochromatography, coelectrophoresis, treatment with phosphodiesterase, chromato- graphy ... metapyrocate-chase and enriched am idase from chloridazon-degrading bacteria by reversed phase high performance liquid chro- matography ...
... and ion-exchange chromatography on DEAE-cellulose. ...
Khaleel Aljaf, Karzan and H.S. Hussain, Faiq (2020) Diethylaminoethyl cellulose (DEAE-C): applications in chromatography and ...
On DEAE cellulose, the T cell-induced LAF fractionated into at least three major peaks and one minor peak. By using ... Sephadex G-75 chromatography of concentrated LAF-containing supernatants from cultures of unstimulated and T cell stimulated ... However, the LPS-induced LAF appeared to lack one of the DEAE peaks of LAF activity observed with the T cell-derived LAF. In ... hydroxylapatite chromatography, two of the major peaks of LAF activity were separated from residual contaminating Lowry ...
9] reported purification of EPS from Morchella aconica using DEAE-Cellulose 52 anion-exchange column chromatography with 5% ... However, Sephadex G-100 column chromatography was used to purify the EPS obtained from Paecilomyces cicadae [10]. Limin et al ... 11] reported purification schizophyllan from Schizophyllum commune using Sephacryl S-500 column chromatography. ... Using Sephadex-G75 column chromatography, EPS was purified by 8.11-fold. Furthermore, the chemical and structural analysis of F ...
Polyamine oxidase (PAO) was purified from lactating mothers milk using dialysis, anion exchange chromatography (DEAE-cellulose ... thin layer chromatography (T.L.C), two-ensional T.L.C and column chromatography. and with comparing the relative retention ... Two proteinous compounds (I and II) had been isolated by gel filtration chromatography of the full saturated precipitate ... By using the technique of gel filtration chromatography (Sephadex G-75) for separating the selenium binding protein inside ...
  • was selected for enzyme purification by ammonium sulfate precipitation, DEAE-cellulose and CM-cellulose column chromatography, respectively. (biomedcentral.com)
  • An extracellular agarase was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography on DEAE-cellulose. (vapormax2018.info)
  • A bromoperoxidase from Gracilaria lemaneiformis was purified to homogeneity using a multi-step process of ammonium sulfate precipitation (AS), dialysis, and DEAE-cellulose 52 anion exchange chromatography. (edu.au)
  • Preparative electrophoresis and column chromatography have been used to isolate putative proteolytic breakdown products of the 172 kDa peptide at 145, 114, 41 and 29 kDa. (umn.edu)
  • Large and small aleurone grains appear very similar in their qualitative antigenic composition.Proteins extracted from mature seeds of Arachis hypogaea appear heterogeneous by chromatography on DEAE-cellulose, ultracentrifugation and polvacrylamide gel electrophoresis (7, 10). (scite.ai)
  • The high purity elastase is homogenous on 15% polyacrylamide electrophoresis at pH 4.5 and on gel exclusion chromatography (G200) at pH 5.0 and 25°C. (elastin.com)
  • Polyamine oxidase (PAO) was purified from lactating mother's milk using dialysis, anion exchange chromatography (DEAE-cellulose), gel filtration and SDS- polyacrylamide electrophoresis. (mosuljournals.com)
  • A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (bvsalud.org)
  • Total creatine kinase activity is measured in sperm homogenates after chromatography on a diethylaminoethyl cellulose (DEAE-32) column. (ijfs.ir)
  • The aqueous extract of Tremella fuciformis was purified using a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column, respectively. (spandidos-publications.com)
  • The anion exchange chromatography purification was a critical step in the purification process, which effectively eliminated the phycobiliprotein and smucilaginous polysaccharides. (edu.au)
  • The calmodulin kinase displayed a characteristic pattern of 60% of beta tubulin phosphorylation on threonine residues.The endogenous association of this kinase with tubulin was established through the isolation of a tubulin-calmodulin kinase complex from rat brain cytosol by sequential chromatography on DEAE-cellulose, Sephacryl S-300 and Fractogel TSK-65F. (yale.edu)
  • Materials and methods Materials SBL was isolated in sequential chromatography on Sephadex G-75 DEAE-cellulose hydroxyapatite and SP-Sepharose as described previously (28). (palomid529.com)
  • The characterization of EPS including monosaccharide composition (high-performance thin-layer chromatography [HPTLC] and HPLC), molecular weight (GPC), rheological behavior, surface morphology (SEM and AFM), as well as IR and nuclear magnetic resonance (NMR) spectra was thoroughly studied. (jabonline.in)
  • High performance liquid chromatography (HPLC) has customarily been the most popular method in forensic and pharmaceutical laboratories for its ability to quantify APIs as well as impurities in a drug sample (3). (spectroscopyonline.com)
  • A high-performance liquid chromatography (HPLC) method has been developed for the determination of isosorbide mononitrate and its related substances in isosorbide mononitrate and sodium chloride injection. (ijpsr.com)
  • The separation patterns of arginyl-tRNA and lysyl-tRNA on MAK column chromatography, of threonyl-tRNA, seryl-tRNA and arginyl-tRNA on Reverse phase type II chromatography and of arginyl, lysyl, seryl and alanyl oligonucleotides on DEAE cellulose chromatography, are shown. (gla.ac.uk)
  • The populations of certain aminoacyl-tRNAs from non-infected and virus infected cells are compared using standard chromatographic techniques such as MAK column chromatography and Reverse phase type II chromatography of aminoacyl"-tRNA, and DEAE cellulose chromatography of T1 RNase digestion products of aminoacyl-tRNA. (gla.ac.uk)
  • The analysis of flavonoides glycoside and its aglycone was made by three chromatographic techniques: thin layer chromatography (T.L.C), two-ensional T.L.C and column chromatography. (mosuljournals.com)
  • Sporozoites of rodent malaria, Plasmodium berghei, and simian malaria, Plasmodium knowlesi and Plasmodium cynomolgi, were partially separated from mosquito debris and microbial contaminants by passage of Anopheles material through a DEAE-cellulose column. (nih.gov)
  • The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6. (researchsquare.com)
  • An inhibitor of the muscle calcium-activated proteinases has been purified from porcine skeletal muscle by using DEAE-cellulose column chromatography, thermal treatment, Sephacryl S-400 column chromatography in 6 M urea and Sephacryl S-300 column chromatography in 6 M urea. (umn.edu)
  • The crude toxic extract was fractionated by silica gel, LH-20 Sephadex gel and DEAE-cellulose column chromatography, and a 27-fold increase in the specific activity of the toxic material was achieved with recovery of 71% of the total activity. (usf.edu)
  • Immunochemical characterizations of aldose reductase and aldehyde reductases I and II, partially purified by DEAE-cellulose (DE-52) column chromatography from human tissues, were carried out by immunotitration, using antisera raised against the homogenous preparations of human and bovine lens aldose reductase and human placenta aldehyde reductase I and aldehyde reductase II. (utmb.edu)
  • In this study, a novel heteropolysaccharide named RGP1-1 was fractionated sequentially by DEAE-52 column and Sephadex G-100 gel column. (biomedcentral.com)
  • Jun 22, · In Ed's laboratory, I first learned about large-scale purification of proteins by observing with Floyd Kennedy, his longstanding chief technician, the purification, via several column chromatography steps, of phosphorylase kinase and other enzymes from several kilograms of skeletal muscle from thirty-five rabbits. (cinemavog-legrauduroi.com)
  • Column equilibration is a time-consuming activity when running chiral chromatography. (vertichrom.com)
  • The primary structure of RGP1-1, including glycosyl linkages, molecular weight, monosaccharide composition, morphology and physicochemical property were conducted by nuclear magnetic resonance (NMR), gas chromatography-mass spectrometer (GC-MS), atomic force microscope (AFM), scanning electron microscope (SEM), differential scanning calorimetry-thermogravimetric analysis (DSC-TG) and so on. (biomedcentral.com)
  • In addition, Raman spectroscopy (4-6), near-infrared (NIR) spectroscopy (7-10), thin-layer chromatography (11), nuclear magnetic resonance (NMR) spectroscopy (12), X-ray fluorescence (XRF) spectrometry (13), and liquid chromatography-mass spectrometry (LC-MS) (14) have all been used in recent years to characterize suspected counterfeit drugs. (spectroscopyonline.com)
  • Cellulose is mainly degraded by cellulase enzyme which is commonly produced by bacteria and fungi [ 6 ]. (biomedcentral.com)
  • Trace trypsin, and chymotrypsin contamination is removed by affinity chromatography. (elastin.com)
  • In all experiments, the preparations from host and virus infected cells are differentially labelled in the amino acid moeity, mixed and subjected to co-chromatography, to provide a valid comparison. (gla.ac.uk)
  • By using the technique of gel filtration chromatography (Sephadex G-75) for separating the selenium binding protein inside erythrocytes, it was found one protein peak for sample treated with selenium for (5, 30 min) and sample without selenium. (mosuljournals.com)
  • It follows the same rationale as cation exchange on familiar chromatography media such as carboxy- and sulfo-based cation exchangers. (bioprocessintl.com)
  • An analytical procedure based on the combined means of purified tRNA isolation from liver cells and ribonucleoside analysis by reverse-phase high performance liquid chromatography coupled with real-time UV-spectrometry (RPLC-UV) was developed for the quantitative analysis of the three Q-derivatives present in total tRNA from liver tissues and liver cell cultures. (cnrs.fr)
  • Kromasil CelluCoat with 3μm particle size enables a higher plate count and resolution for analytical chromatography. (vertichrom.com)
  • Thanks to a product characteristic like the absence of pressure limits, you can run analytical chromatography at very high flow rates and save time. (vertichrom.com)
  • This makes it possible to run analytical chromatography a lot faster. (vertichrom.com)
  • For many years, several studies on isolation and characterization of cellulose degrading bacteria from industrial wastes indicated that only a small number of bacteria can produces large amount of bioactive compounds that are capable of complete hydrolysis of crystalline cellulose in vitro [ 2 , 14 ]. (biomedcentral.com)
  • Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC) agar plate were identified as cellulase producing bacteria. (biomedcentral.com)
  • Then 100 µl of the solution was transferred into 1l of carboxymethyl cellulose (CMC) agar media plates containing 0.5 g KH 2 PO 4 , 0.25 g MgSO 4 , 0.25 g cellulose and 2 g gelatin for the enhancement of the bacterial activity. (biomedcentral.com)
  • They are involved in many metabolic pathways, in the biosynthesis and degradation of various biomolecules such as bacterial exopolysaccharides, starch, cellulose and lignin, and in the glycosylation of proteins and lipids. (mdpi.com)
  • However, chromatography-based techniques require extensive sample preparation that is best suited to highly trained staff in forensic or pharmaceutical laboratories, and some spectroscopic techniques, such as NIR, may require chemometric modeling. (spectroscopyonline.com)
  • DEAE-cellulose chromatography showed increased ornithine-decarboxylase antizyme activity in liver microsomal fractions from treated male animals only. (cdc.gov)
  • The elastolytic effluent is chromatographed at pH 5.0 on CM-cellulose recovering the highest specific activity by elution with salt gradient. (elastin.com)
  • By using hydroxylapatite chromatography, two of the major peaks of LAF activity were separated from residual contaminating Lowry positive material. (aai.org)
  • However, the LPS-induced LAF appeared to lack one of the DEAE peaks of LAF activity observed with the T cell-derived LAF. (aai.org)
  • The DEA does not allow our program to provide quantitative results. (drugsdata.org)
  • Only direct sequencing of purified RNA molecules and high-performance liquid chromatography mass spectrometry analysis of purified RNA fragments allow determination of both the type and location of a given modified nucleotide within an RNA of 50-150 nt in length. (cnrs.fr)
  • Consequently, we prepared a peptide hydrolysate by shaving and hydrolysis of surface proteins using trypsin, and the origin of peptides was checked by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. (calbinotox.fr)
  • Sephadex G-75 chromatography of concentrated LAF-containing supernatants from cultures of unstimulated and T cell stimulated P388D 1 cells demonstrated that the cell line LAF had a m.w. of approximately 16,000. (aai.org)
  • An analysis of the soluble proteins from the adult and calf bovine lens epithelial cells by DEAE-cellulose fractionation has shown that these cells do not contain the γ-crystallins, but do. (cinemavog-legrauduroi.com)
  • The 2X elastase is chromatographed on DEAE-cellulose at pH 8.8 to remove anionic non-specific protein. (elastin.com)
  • One challenge facing the purification of therapeutic glycoproteins by affinity chromatography is creating ligands specific for the glycan moiety. (sigmaaldrich.com)
  • Affinity chromatography of glycoproteins is currently conducted with immobilized lectins or boronates, although biomimetic ligands could present a more desirable option. (sigmaaldrich.com)
  • 1o5 n KI-PBS was used in the purification of PAPP-A by antibody affinity chromatography followed by DEAE-cellulose ion exchange and Sepharose 6B gel filtration. (gla.ac.uk)
  • The chromatography steps can include one or more of the following chromatographic procedures: ion exchange chromatography, affinity chromatography, and hydrophobic interaction chromatography. (justia.com)
  • After a suitable period of time, the pH can be adjusted toward a more neutral or basic pH and in certain embodiments the sample will be subjected to one or more chromatographic steps, including, but not limited to affinity chromatography, ion exchange chromatography, and hydrophobic interaction chromatography. (justia.com)
  • In one embodiment, the affinity chromatography step comprises subjecting the primary recovery sample to a column comprising a suitable affinity chromatographic support. (justia.com)
  • The calcium-dependent regulatory protein (CDR)-Ca2+ sensitive cyclic nucleotide phosphodiesterase was purified to apparent homogeneity from bovine heart by using ammonium sulfate fractionation, DEAE-cellulose chromatography, and CDR-Sepharose affinity chromatography. (umn.edu)
  • QuikPrep ® SpinColumns™ for anion exchange chromatography are filled with a positively charged resin to attract negatively charged molecules from samples. (harvardapparatus.com)
  • C1q-p was found to partition with IgG during precipitation by ammonium sulfate and low ionic strength buffer as well as during column chromatography on DEAE-cellulose and G-200 Sephadex. (jimmunol.org)
  • Ca2+-calmodulin-dependent protein kinase II (CaM-kinase II) purified from rat brain by ammonium sulfate precipitation and ion-exchange chromatography on DEAE-cellulose is highly enriched in the cytoskeletal fraction. (socialtinkering.org)
  • The kit uses Thin Layer Chromatography (TLC) and was designed specifically for testing marijuana plants and oils. (cannalyticssupply.com)
  • The Cannalytics Test Kit is a "Thin Layer Chromatography" testing kit designed specifically for testing medical marijuana. (cannalyticssupply.com)
  • Thin-layer chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds. (cannalyticssupply.com)
  • A linearly increasing phosphate gradient was then used to separate isoenzyme GT-II from GT-I. The isoenzymes were further purified by sequential chromatography on α-lactalbumin/Sepharose 4B and N-acetylgucosamine/Sepharose 4B affinity columns achieving a final purification of 5400-fold for GT-II and 4300-fold for GT-I. The separated isoenzymes showed homogeneity by polyacrylamide and sodium dodecyl sulfate polyacrylamide gel electrophoresis. (elsevier.com)
  • Ion exchange chromatography (IEX) is an effective method of sample purification and fractionation based on molecular charge. (harvardapparatus.com)
  • Background: There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. (ijbiotech.com)
  • Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consuming method and it needs machinery and plant. (ijbiotech.com)
  • Purification was achieved by DEAE-cellulose ion-exchange chromatography and gel-permeation chromatography. (nih.gov)
  • In addition, each tablet contains the following inactive ingredients: lactose monohydrate, magnesium stearate, microcrystalline cellulose, and pregelatinized starch. (nih.gov)
  • In addition to the active ingredient lovastatin, USP, each tablet contains the following inactive ingredients: lactose monohydrate, magnesium stearate, microcrystalline cellulose, and pregelatinized corn starch. (nih.gov)
  • DEAE-Sephadex chromatography indicated three Cx components. (ubc.ca)
  • elaeoselini by chromatography on DEAE-cellulose 32 and Sephadex G-100 column were found to consist of only D-glucose as monosaccharide constituent. (unipa.it)
  • The inhibitor was purified by DEAE-cellulose chromatography followed by gel permeation on Sephadex G-75. (jabonline.in)
  • Chromatography of the solubilized preparation on Sephadex G-100 WAS CHARACTERIZED BY 125-I-glucagon binding and fluoride-stimulatable adenylate cyclase activity appearing in the fractions consistent with the void volume, suggesting a molecular weight greater than 100,000 for the receptor-adenylate cyclase complex. (elsevier.com)
  • This protein called "Proteinase I" was obtained using a gel filtration chromatography on Sephadex G-100 at pH 6.5, 0.1 M Ammonium acetate buffer, followed by ion exchange chromatography on DEAE-Cellulose at pH 7.5 and re-chromatographed on DEAE-Cellulose at pH 9.0 and 7.8 in Tris-HC1 buffer. (edu.pe)
  • A glucosyltransferase was isolated from im mature "cherry" tom atoes and was partially purified (200-fold) by am m onium sulphate precipitation and successive chrom atography on Sephadex G-100 and DEAE-cellulose columns. (mpg.de)
  • An analytical procedure based on the combined means of purified tRNA isolation from liver cells and ribonucleoside analysis by reverse-phase high performance liquid chromatography coupled with real-time UV-spectrometry (RPLC-UV) was developed for the quantitative analysis of the three Q-derivatives present in total tRNA from liver tissues and liver cell cultures. (cnrs.fr)
  • High-performance liquid chromatography is a form of column chromatography used frequently in analytical chemistry. (cannalyticssupply.com)
  • Methods: The crude extract from soil fungal isolate cultures is subjected to salt precipitation, dialysis and DEAE cellulose chromatography followed by amylase extraction and is incubated with divalent metal ions (i.e. (eurekaselect.com)
  • All of the fractions exhibiting galactosyltransferase activity were pooled, concentrated by (NH 4 ) 2 SO 4 precipitation, resuspended, and placed on a DEAE-cellulose column. (elsevier.com)
  • The other method for protein separation is ion exchange chromatography (17, 18). (ijbiotech.com)
  • So far many commercially available gels have been produced for ion exchange chromatography for which scaling up for industrial scale is not so easy. (ijbiotech.com)
  • A novel paper combining cellulose and large pore silica gel. (thelabwarehouse.com)
  • It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose immobilized onto a flat, inert carrier sheet. (cannalyticssupply.com)
  • Strain M-7 did not produce cellulase when grown on any carbon source other than cellulose substrates. (ubc.ca)
  • GT-II contained twice as much hexosamine which was identified as glucosamine by gas-liquid chromatography. (elsevier.com)
  • Restek develops and manufactures GC and LC columns, reference standards, sample prep materials, and accessories for the international chromatography industry. (stim-design.de)
  • 2016-2-1u2002·u2002Sipunculus nudus Linnaeus polysaccharide (SNP) was purified from S. nudus L. via NaOH extraction, trichloroacetic acid deproteination, DEAE-cellulose 52 and Sephacryl S-300 chromatography. (rimbo.info)
  • The rapid production and high activity of cellulases from this organism strongly support the basic premise that increased hydrolysis of cellulose is possible at elevated temperature. (ubc.ca)
  • An enrichment procedure led to the isolation, by the cellulose roll tube method, of a number of actively cellulolytic anaerobic thermophilic bacteria. (ubc.ca)
  • DEAE-cellulose chromatography showed increased ornithine-decarboxylase antizyme activity in liver microsomal fractions from treated male animals only. (cdc.gov)
  • In a particular aspect, methods herein employ an acidification step followed by one or more chromatography steps. (justia.com)
  • The addition of cellobiose (0.3%) and glucose (0.4%) prevented cellulose hydrolysis in cellulose medium. (ubc.ca)
  • The cellulase(s) from strain M-7 were extra-cellular, produced during exponential growth but were not free in the growth medium until 50% of the cellulose was hydrolyzed. (ubc.ca)
  • The first documented application of mass spectrometry (MS) in IBD occurred in 1982, when an absolute targeted quantification of small molecules was carried out by Nishida and colleagues using gas chromatography/mass spectrometry (GC/MS) with an internal standard calibration curve and stable isotope labeling to describe bile acid circulation impairment in CD patients after ingestion of deuterium labeled chenodeoxycholic acid. (medscape.com)
  • Both C₁, cellulase (degrades native cellulose) and Cx cellulase (β-1,4-glucanase) activities in strain M-7 cultures were assayed by measuring the liberation of reducing sugars, using dinitrosalicylic acid. (ubc.ca)
  • DEAE-cellulose chromatography revealed two aromatic aminotransferase activities that were distinct from prephenate aminotransferase and which did not require the three protectants for stability. (unipr.it)
  • Glucose and cellobiose were the only soluble products liberated by the cellulase from cellulose. (ubc.ca)
  • For more information about Diethanolamine (DEA) , please contact our technical experts. (nouryon.com)
  • The DEA does not allow our program to provide quantitative results. (drugsdata.org)