Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatographic techniques in which the mobile phase is a liquid.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The rate dynamics in chemical or physical systems.
An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)
A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.
Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A series of steps taken in order to conduct research.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The chemical and physical integrity of a pharmaceutical product.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The process of cleaving a chemical compound by the addition of a molecule of water.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins prepared by recombinant DNA technology.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Compounds in which a methyl group is attached to the cyano moiety.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
The characteristic 3-dimensional shape of a carbohydrate.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
High-molecular-weight insoluble polymers that contain functional cationic groups capable of undergoing exchange reactions with anions.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Proteins found in any species of bacterium.
Established cell cultures that have the potential to propagate indefinitely.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Pyrolysis of organic compounds at the temperature of a hydrogen-air flame to produce ionic intermediates which can be collected and the resulting ion current measured by gas chromatography.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Transport proteins that carry specific substances in the blood or across cell membranes.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
Elements of limited time intervals, contributing to particular results or situations.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Antibodies produced by a single clone of cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
Measurement of the intensity and quality of fluorescence.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Inorganic salts of sulfuric acid.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A very strong halogenated derivative of acetic acid. It is used in acid catalyzed reactions, especially those where an ester is cleaved in peptide synthesis.
The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.
A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Compounds containing the -SH radical.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
The presence of organisms, or any foreign material that makes a drug preparation impure.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.
A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
Derivatives of GLUCURONIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the 6-carboxy glucose structure.
An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.

R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. (1/4718)

Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide assay, but retained activity as catalysts of a proline-limited protein folding reaction [Scholz, C. et al. (1997) FEBS Lett. 414, 69-73]. A reinvestigation revealed that in fact these two mutations strongly decrease the prolyl isomerase activity of Cpr3 in both the peptide and the protein-folding assay. The high folding activities found previously originated from a contamination of the recombinant Cpr3 proteins with the Escherichia coli protein SlyD, a prolyl isomerase that co-purifies with His-tagged proteins. SlyD is inactive in the peptide assay, but highly active in the protein-folding assay.  (+info)

Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme. (2/4718)

The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a potential N-terminal signal peptide that is consistent with its proposed periplasmic location. The analysis of the deduced amino acid sequence indicated that the gene sequence has a high homology (90% identity) to the Azotobacter vinelandii gene sequence, which has very recently been deposited in the GenBank database, and that it has 64% identity to the Pseudomonas aeruginosa gene sequence but that it has rather low homology (15 to 22% identity) to the gene sequences encoding alginate lyase in other bacteria. The A. chroococcum AlgL protein was overproduced in Escherichia coli and purified to electrophoretic homogeneity in a two-step chromatography procedure on hydroxyapatite and phenyl-Sepharose. The kinetic and molecular parameters of the recombinant alginate lyase are similar to those found for the native enzyme.  (+info)

EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. (3/4718)

This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstrated first by RT-PCR analysis of lacrimal gland RNA using a set of specific primers and second by Northern blot analysis of rat lacrimal gland mRNA. A rabbit polyclonal antibody (rEGF2) directed against rat submaxillary gland EGF was used to detect EGF-containing proteins by RIA. Results indicate that the rat lacrimal gland does not contain detectable soluble and mature EGF but that the EGF immunoreactivity is associated with the membrane-enriched fraction. Analysis of the detergent-solubilized membrane proteins by gel filtration shows that membrane-associated EGF immunoreactivity was present as a high-molecular-mass protein. Moreover, as shown by Western blot analysis, a specific anti-rat EGF precursor antibody (ppEGF1) can immunoprecipitate a 152-kDa EGF-containing protein. Taken together, these results demonstrate for the first time both EGF precursor gene transcription and EGF precursor protein expression in a lacrimal tissue, i.e., the rat exorbital lacrimal gland. The demonstration that EGF appears to be stored only as its full-length membrane precursor may provide important information to study the regulation of its secretory process.  (+info)

Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes: chromatographic measurement of their membrane partition coefficients. (4/4718)

Unilamellar liposomes of small or large size, SUVs and LUVs, respectively, were stably immobilized in the highly hydrophilic Sepharose 4B or Sephacryl S-1000 gel beads as a membrane stationary phase for immobilized liposome chromatography (ILC). Lipophilic cations of triphenylmethylphosphonium and tetraphenylphosphonium (TPP+) have been used as probes of the membrane potential of cells. Interaction of TPP+ and triphenylalkylphosphonium homologues with the immobilized liposomal membranes was shown by their elution profiles on both zonal and frontal ILC. Retardation of the lipophilic cations on the liposome gel bed was increased as the hydrophobicity of the cations increased, indicating the partitioning of lipophilic cations into the hydrocarbon region of the membranes. The cations did not retard on the Sepharose or Sephacryl gel bed without liposomes, confirming that the cations only interact with the immobilized liposomes. Effects of the solute concentration, flow rate, and gel-matrix substance on the ILC were studied. The stationary phase volume of the liposomal membranes was calculated from the volume of a phospholipid molecule and the amount of the immobilized phospholipid, which allowed us to determine the membrane partition coefficient (KLM) for the lipophilic cations distributed between the aqueous mobile and membrane stationary phases. The values of KLM were generally increased with the hydrophobicity of the solutes increased, and were higher for the SUVs than for the LUVs. The ILC method described here can be applied to measure membrane partition coefficients for other lipophilic solutes (e.g., drugs).  (+info)

Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis. (5/4718)

We have constructed a library of DNA fragments heavily methylated in human adenocarcinomas of the lung to permit the comprehensive isolation of methylated CpG islands in cancer. Heavily methylated genomic DNA fragments from tumors of nine male patients were enriched using a methylated DNA binding column and used for construction of the library. From this library, DNA fragments having properties of CpG islands were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0.3% of the library were analyzed, and nine DNA fragments were identified as being associated with CpG islands that were methylated in tumor DNA. One CpG island was methylated specifically in tumor DNA, whereas the remaining eight CpG islands were methylated both in normal and tumor DNA derived from the same patients. Our results suggest that the number of CpG islands methylated specifically in tumors is not large. The library, which contains DNA fragments from methylated CpG islands comprehensively, is expected to be valuable when elucidating epigenetic processes involved in carcinogenesis.  (+info)

Deamidation of alpha-A crystallin from nuclei of cataractous and normal human lenses. (6/4718)

PURPOSE: To quantitate the extent of deamidation of asparagine-101, glutamine-50, and glutamine-6 of alpha-A crystallin in the nucleus from human cataractous and normal lenses. METHODS: Reverse phase chromatography was used to prepare alpha-A crystallin from total proteins of the nucleus from cataractous and age-matched normal human lenses. Synthetic peptides were made corresponding to the expected amidated and deamidated tryptic fragments containing asparagine-101, glutamine-50, and glutamine-6. The peptides were used to identify and quantitate amidated and deamidated forms of tryptic fragments from alpha-A crystallin eluting from a reverse phase column. RESULTS: Significant amounts of deamidation of asparagine-101 and glutamine-50, but not glutamine-6, were present in alpha-A crystallin from nuclear sections of both cataractous and age-matched normal lenses. Quantitative analysis of tryptic peptides containing these residues indicated no statistical difference in deamidation in cataractous versus normal lenses. CONCLUSIONS: There was no significant difference in the extent of deamidation of asparagine-101, glutamine-50, and glutamine-6 for alpha-A crystallin, purified from the nucleus of cataractous versus age-matched normal lenses. These results strongly suggest that deamidation of these residues does not play a role in the biogenesis of human nuclear cataract.  (+info)

Purification and properties of bovine pituitary follitropin. (7/4718)

A reproducible procedure was developed for the purification of follitropin from frozen bovine pituitary glands. The method involved precipitation with (NH4)2SO4 and acetone, followed by ion-exchange column chromatography on CM-cellulose and DEAE-cellulose and gel filtration on Sephadex G-100. A specific radioligand-receptor assay for follitropin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified bovine follitropin as measured by Steelman-Pohley bioassay was 164 times that of NIH-FSH-S1 standard preparation. They yield of bovine follitropin was 2.9 mg/kg of frozen pituitary glands. Electrophoretically, bovine follitropin was more acidic in nature and migrated further towards the anode than lutropin and thyrotropin. The elution volume of bovine follitropin by gel filtration on Sephadex G-100 was very similar to that of bovine lutropin. The amino acid composition of bovine follitropin was similar to that of sheep and human follitropin, being rich in lysine, aspartic acid, threonine, serine, glutamic acid and half-cystine.  (+info)

The selective isolation of the uterine oestradiol-receptor complex by binding to oligo(dT)-cellulose. The mediation of an essential activator in the transformation of cytosol receptor. (8/4718)

The [3H]oestradiol-receptor complex was selectively isolated from rat uterus cytosol by column chromatography on oligo(dT)-cellulose. Optimal conditions are described for the binding of the complex to oligo(dT)-cellulose, which is shown to be similar to its binding to DNA-cellulose. The cytosol complex has an apparent mol. wt. of 50,000-60,000 in high salt concentrations, as determined by Sephadex G-100 chromatography. This corresponds to the 4S cytoplasmic oestradiol receptor. In binding to oligo(dT)-cellulose the receptor is transformed into a form with an apparent mol.wt. of 100,000-120,000, corresponding to the 5S nuclear receptor complex. This transformation mimics the conversion in vivo of the cytoplasmic oestradiol receptor into the nuclear form. The binding of the complex to oligo(dT)-cellulose as a 5S nuclear form is unequivocally demonstrated to require the mediation of an activating present in the cytosol. The requirement for an activating factor is discussed in relation to reports that nuclear binding of the oestradiol-receptor complex is not dictated solely by the availability of the cytoplasmic oestradiol receptor.  (+info)

Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another. In this type of chromatography, hydrophobic groups such as phenyl, octyl, or butyl, are attached to the stationary column. Proteins that pass through the column that have hydrophobic amino acid side chains on their surfaces are able to interact with and bind to the hydrophobic groups on the column. HIC separations are often designed using the opposite conditions of those used in ion exchange chromatography. In this separation, a buffer with a high ionic strength, usually ammonium sulfate, is initially applied to the column. The salt in the buffer reduces the solvation of sample solutes thus as solvation decreases, hydrophobic regions that become exposed are adsorbed by the medium. ...
The design of gradient simulated moving bed (SMB) chromatographic processes requires an appropriate selection of the chromatographic system followed by the determination of adsorption isotherm parameters in the relevant range of mobile phase conditions. The determination of these parameters can be q …
In this eBook devoted to Practical Copolymer Analysis using GPC/SEC and Related Techniques, experts discuss how several GPC/SEC methods may improve copolymer sample characterization. Learn about dual-detection methods, polymer liquid adsorption chromatography, 2D chromatography, and more ...
A convenient method is described for two-dimensional amino acid chromatography on micro scale chromatograms of 5 × 5 cm. Despite the short migration paths, excellent separation is obtained. The time of development is short and the technical procedure very simple. Extension to other classes of compounds is possible. show less ...
TY - JOUR. T1 - Chromatography in plasma fractionation. T2 - benefits and future trends. AU - Burnouf, T.. PY - 1995/2/3. Y1 - 1995/2/3. N2 - Industrial-scale chromatographic fractionation and purification methods have been used increasingly in the last few years for plasma fractionation. This has resulted in the development of a new generation of therapeutic plasma derivatives, especially coagulation factors, protease inhibitors and anticoagulants. Implementation and combination of ion-exchange, affinity and size-exclusion chromatography have allowed the development of new therapeutic products with improved purity and safety for treating congenital or acquired plasma protein deficiencies in patients. More recently, the benefit of chromatographic purification of plasma proteins in the removal of plasma-borne viruses has been revealed. Development of packing materials with improved characteristics for industrial applications, including higher capacity and rigidity, should further promote the use ...
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The Intrada Amino Acid column has been shown to be a viable solution for the analysis of amino acids by LC-MS without derivatization.
Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm) using 80 methanol as an eluent. The relevant fractions were pooled and
Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns
Read user reviews, compare products & request pricing from manufacturers of size exclusion chromatography (SEC) products, including GFC & GPC columns.
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
Paper Chromatography - Free download as Powerpoint Presentation (.ppt), PDF File (.pdf), Text File (.txt) or view presentation slides online. Paper Chromatography Biochemistry
Column Chromatography Science Project: Investigate whether a homemade column chromatography setup can be used to separate and isolate the different food colorings that are in grape soda.
This webcast presents a series experiments in which in-process mAb material was spiked with MVM-MVP and processed through Anion Exchange and Cation Exchange, and a a viral clearance study was performed on Hydrophobic Interaction Chromatography (HIC) resins
A.T. Hanke, M.E. Klijn, P.D.E.M. Verhaert, L.A.M. van der Wielen, M. Ottens, M.H.M. Eppink, E.J.A.X. van de Sandt, Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties, Biotechnol Prog. (2015) 1520-6033, http://dx.doi.org/10.1002/btpr.2219 ...
Buy High Resolution Chromatography for $466.99 at Mighty Ape NZ. The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give re...
Invited Talk at 2002 ABRF Meeting: Determination of Molecular Masses of Proteins in Solution: Implementation of an HPLC Size Exclusion Chromatography and Laser
标签蛋白的表达是研究目标蛋白的一种重要办法。而这个可能会影响到蛋白结构和功能研究的蛋白标签往往需要被去除。该视频介绍了几种用于标签蛋白柱上酶切的试剂,以及在使用过程中的一些小技巧。. The expression of tagged proteins is the major source of proteins for research purposes. It is often recommended to remove the tag, which might otherwise interfere with protein function or interactions. This video will give you some examples of automated on-column tag removal with different cleavage agents ...
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Immunochromatography - General Phytopathology Immunochromatography - General Phytopathology Agro-industry General phytopathology Immunochromatography For.
Protein kinase C in the developing rat brain was investigated by a biochemical assay and by light-microscopic immunocytochemistry. The protein kinase was resolved on hydroxyapatite column chromatography into 3 fractions, designated types I, II, and III. Type I, with structure encoded by a gamma-sequence, was not detected early postnatally, maintained a low level of activity during the first week, which increased gradually, and reached its maximum around postnatal day 28. This type of enzyme was expressed specifically in nervous tissues, and was not found in any other tissues thus far tested. Type II enzyme activity, a mixture of the 2 subspecies encoded by the beta I- and beta II-sequences, was found at birth, increased rapidly, and reached a plateau level between postnatal days 14 and 28. This type was the predominant subspecies of protein kinase C in the brain. Type III, its structure encoded by the alpha-sequence, was also detected at birth, and reached its maximum level on postnatal day 7. ...
Introduction. Identification of amino acids by using paper chromatography Aim To separation and identification of amino acids by using paper chromatography Introduction Chromatography is a techniques separation of mixtures It involves passing the sample, a mixture which contains the analyte, in the mobile phase, often in a stream of solvent, through the stationary phase. The stationary phase retards the passage of the components of the sample. When components pass through the system at different rates they become separated in time, like runners in a mass-start foot race. Each component has a characteristic time of passage through the system, called a retention time. Chromatographic separation is achieved when the retention time of the analyte differs from that of other components mixtures in the sample. There are many types chromatography but there are four main types which are Liquid Chromatography Liquid Chromatography this is used in the world to test water samples to look for pollution ...
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HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.
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hai dear bloggers, i m here with my blog titled as COLUMN CHROMATOGRAPHY from team PHARMA WARRIORS . COLUMN CHROMATOGRAPHY column chromatography is a technique in which a column of stationary phase is used.based upon the stationary phase nature,this column chromatography is further divided into 2 types.they are if a
Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.
Dispersion due to resistance to mass transfer in the stationary phase is exactly analogous to that in the mobile phase. Solute molecules close to the interface will leave the stationary phase and enter the mobile phase before those that have diffused further into the stationary phase and have a longer distance to diffuse back. Thus, as those molecules that were close to the surface will be swept along in the moving phase, they will be dispersed from those molecules still diffusing to the surface. The dispersion resulting from the resistance to mass transfer in the stationary phase is depicted in figure 23. Molecules 1 and 2 (the two closest to the surface) will enter the mobile phase and begin moving along the column. Their movement will continue while molecules 3 and 4 diffuse to the interface at which time they will enter the mobile phase and start following molecules 1 and 2 down the column. ...
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Omnifit Low-Pressure Chromatography Columns Column Assemblies w/ Two Adjustable Endpieces : These precision glass columns are ideal for low- and mid-
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Prepacked Chromatography Columns Market was US$1.74 bn in 2016 and is poised to reach US$3.34 bn by 2024, expand at a CAGR of 8.4% therein
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Tartaric, Malic & Lactic acid chromatography papers. Replacement for Chromatography Kit (PCKV). Sold by Presque Isle Wine Cellars in North East, PA, experts in wine making and winemaking supplies, and now brewing supplies.
Definition of Paper chromatography with photos and pictures, translations, sample usage, and additional links for more information.
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The core houses the inner frit, through which the eluent percolates and exits at the base of the column to a detector and hence to a fraction collector. The outer frit constitutes the column inlet, and consequently the sample has initially an extremely large area of stationary phase with which to interact. This renders the loading capacity of the radial flow column also very high. It is interesting to note, that as the solute progress radially through the stationary phase bed towards the center, the effective cross-sectional area of the column will become smaller. Consequently, the plate volume of the column will decrease (see Plate Theory and Extensions ) as the solute moves to the center which will result in the solute being concentrated. However, as the solute bands progressively decrease in concentration due to normal dispersion processes (see Dispersion in Chromatography Columns ), this counteracts the concentration effect from reduced bed cross-section and prevents the column packing from ...
Chromatography are Cost-effective and innovative chromatographic purification platforms. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step. Explore our solutions.
Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed …
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A major Liquid Chromatography mode in which samples are separated by virtue of their size in solution. Also known as size-exclusion, gel permeation, gel fi
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Yes there was a change. AFAIK it is related to multi selection behaviour. I guess you configured the selection model to not allow multiple selection. Before column selection did not respect that configuration. So the behaviour now should be correct while before it was wrong ...
Hi I have attached a sheet as an example, as i am trying to Divide a cell by the number of populated cells in that column e.g column (A) has a table of 16 but only has 13 populated cells in that column, the sum would be something like this =sum(A18/13. Sometimes the column will have less and at times more populated. Hopefully its clear to you Thanks
Hi, I have sequential numbers below in column A of excel. M66.211 M66.212 M66.213 M66.214 M66.215 M66.216 M66.217 M66.218 M66.219 M66.811 M66.812 M66.
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Stool sugar chromatography[edit]. Chromatography can be used to separate and identify undigested sugars present in faeces. ...
Affinity chromatography[edit]. Affinity chromatography[6] is a method of separating biochemical mixtures, based on a highly ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... Wilchek, M. (1972). "Purification of the heme peptide of cytochrome c by affinity chromatography". Anal. Biochem. 49 (2): 572- ... 1987 Wolf Prize in Medicine, jointly with Pedro Cuatrecasas, "for the invention and development of affinity chromatography and ...
Chromatography[edit]. Chromatographic methods of removing viruses are great for purifying the protein and are also effective ... Chromatography. These extraction processes are considered "traditional processes" because they do not chemically affect the ...
Similar to gas chromatography MS (GC-MS), liquid chromatography-mass spectrometry (LC/MS or LC-MS) separates compounds ... Main article: Gas chromatography-mass spectrometry. A common combination is gas chromatography-mass spectrometry (GC/MS or GC- ... The duty cycle of IMS is short relative to liquid chromatography or gas chromatography separations and can thus be coupled to ... Eiceman, G.A. (2000). Gas Chromatography. In R.A. Meyers (Ed.), Encyclopedia of Analytical Chemistry: Applications, Theory, and ...
SPE and chromatography[edit]. SPE is not a method of chromatography, except in the broadest, simplest sense. It is an ... Hennion, Marie-Claire (1999). "Solid-phase extraction: method development, sorbents, and coupling with liquid chromatography". ... Journal of Chromatography A. 856 (1-2): 3-54. doi:10.1016/S0021-9673(99)00832-8. ISSN 0021-9673. PMID 10526783.. .mw-parser- ... with aims separate from chromatography, and so has a unique niche in modern chemical science. ...
FTIR as detector in chromatography[edit]. The speed of FTIR allows spectra to be obtained from compounds as they are separated ... Liquid chromatography fractions are more difficult because of the solvent present. One notable exception is to measure chain ... However this technique is little used compared to GC-MS (gas chromatography-mass spectrometry) which is more sensitive. The GC- ... branching as a function of molecular size in polyethylene using gel permeation chromatography, which is possible using ...
Gas chromatography-combustion-IRMS[edit]. The gas chromatography-combustion-IRMS is a way to detect any variations in the ...
... (GC-MS) is an analytical method that combines the features of gas-chromatography and mass ... Pyrolysis-gas chromatography-mass spectrometry. References[edit]. *^ O. David Sparkman; Zelda Penton; Fulton G. Kitson (17 May ... Niessen, W. M. A. (2001). Current practice of gas chromatography-mass spectrometry. New York, N.Y: Marcel Dekker. ISBN 978-0- ... The first on-line coupling of gas chromatography to a mass spectrometer was reported in 1959.[2][3][4] The development of ...
Paper chromatography. Reversed-phase chromatography. Size-exclusion chromatography. Thin-layer chromatography. Two-dimensional ... Affinity chromatography. Column chromatography. Displacement chromatography. Electrochromatography. Gas chromatography. High- ... performance liquid chromatography. Capillary electrochromatography. Ion chromatography. Micellar electrokinetic chromatography ... chromatography. Van Deemter equation. Chapter 3. Fundamental Spectroscopy. Raman spectroscopy. Rayleigh scattering. ...
In liquid chromatography, the eluent is the liquid solvent; in gas chromatography, it is the carrier gas.[1] ... In a liquid chromatography experiment, for example, an analyte is generally adsorbed, or "bound to", an adsorbent in a liquid ... For instance, a mixture of amino acids may be separated by ion-exchange chromatography. Under a particular set of conditions, ... Brown, Phillis (2001). Advances in chromatography. CRC Press. p. 36. ISBN 0-8247-0509-2. .. ...
2007). "Human exhaled air analytics: Biomarkers of diseases". Biomedical Chromatography. 21 (6): 553-566. doi:10.1002/bmc.835. ... Journal of Chromatography A. 1026 (1-2): 9-18. doi:10.1016/j.chroma.2003.11.069. PMID 14870711.. ... Journal of Chromatography A. 1216 (3): 540-566. doi:10.1016/j.chroma.2008.10.117. PMID 19019381.. ... of volatile organic compounds emitted by a naturally aged book using solid-phase microextraction/gas chromatography/mass ...
Journal of Chromatography B. 831 (1-2): 110-5. doi:10.1016/j.jchromb.2005.11.050. PMID 16377264.. ... chlorinated organic chemicals in breast milk using automated on-line column-switching-high performance liquid chromatography- ...
Analysis by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry". Biomedical Chromatography ...
Biomedical Chromatography. 12 (6): 350-356. doi:10.1002/(SICI)1099-0801(199811/12)12:6,350::AID-BMC761,3.0.CO;2-B.. ...
Biomedical Chromatography. 27: 1690-1700. doi:10.1002/bmc.2981.. ...
Finger, Andreas; Kuhr, Susanne; Engelhardt, Ulrich (1992). "Chromatography of tea constituents". Journal of Chromatography. 624 ... "Analysis of derivatized and underivatized theanine enantiomers by high-performance liquid chromatography/atmospheric pressure ...
Journal of Chromatography. 422: 309-14. doi:10.1016/0378-4347(87)80468-1. PMID 3437019.. ...
High-performance liquid chromatography (HPLC): The HbA1c result is calculated as a ratio to total hemoglobin by using a ... "Glycated Hemoglobin in Uremic Patients as Measured by Affinity and Ion-Exchange Chromatography" (PDF). clinchem.com. Retrieved ... Huisman TH, Martis EA, Dozy A (1958). "Chromatography of hemoglobin types on carboxymethylcellulose". J. Lab. Clin. Med. 52 (2 ... Boronate affinity chromatography. In the United States, HbA1c testing laboratories are certified by the National ...
For example, gas chromatography-mass spectrometry, gas chromatography-infrared spectroscopy, liquid chromatography-mass ... Chromatography, electrophoresis and Field Flow Fractionation are representative of this field. Hybrid techniques[edit]. ... Bartle, Keith D.; Myers, Peter (2002). "History of gas chromatography". TrAC Trends in Analytical Chemistry. 21 (9-10): 547. ... Most often the other technique is some form of chromatography. Hyphenated techniques are widely used in chemistry and ...
"Gas Chromatography-Mass Spectrometry". American Chemical Society. Retrieved 19 Nov 2019.. *^ "Marine Surplus Seller, Issues 13- ... Harrington of Bendix Aviation in the 1950s led to the combination of gas chromatography and mass spectrometry, and the ... development of Gas chromatography-mass spectrometry instrumentation. Beginning in the 1960s, Bendix produced scientific ...
"Gas Chromatography-Mass Spectrometry". American Chemical Society. Retrieved 19 November 2019.. *^ a b c d "J.J. Thomson - ...
Determination of Dithionite (S2O42−) and Hydroxymethanesulphinate (HOCH2SO2−; Rongalite) by Ion-Pair Chromatography". Journal ...
Shpak AV, Appolonova SA, Semenov VA (2005). "Validation of liquid chromatography-electrospray ionization ion trap mass ... Appolonova, Svetlana S.; Shpak, Alexey V.; Semenov, Vitaliy (2004). "Liquid chromatography-electrospray ionization ion trap ... it has been shown that presence of amphetamine in prior studies was an artifact of gas chromatography method.[21] More recent ... but this was based on older literature that relied on gas chromatography as an analytical method. Subsequently, with the advent ...
Hager DA, Jin DJ, Burgess RR (August 1990). "Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure ...
"Investigations on the in vitro racemization of thalidomide by high-performance liquid chromatography". Journal of ...
Journal of Chromatography A. 315: 201-210. doi:10.1016/S0021-9673(01)90737-X.. Quoted from a campaign site giving no details of ...
Journal of Chromatography B. 2006, 831 (1-2): 110-115. PMID 16377264. doi:10.1016/j.jchromb.2005.11.050.. ... chlorinated organic chemicals in breast milk using automated on-line column-switching-high performance liquid chromatography- ...
These fractions are separated using chromatography.[20] Generally, the fullerenes are dissolved in hydrocarbon or halogenated ...
Journal of Liquid Chromatography & Related Technologies. 20 (16-17): 2857-2872. doi:10.1080/10826079708005597.. ...
The CRFs in thin layer chromatography characterize the equal-spreading of the spots. The ideal case, when the RF of the spots ... In high performance liquid chromatography the CRF is calculated from various parameters of the peaks of solutes (like width, ... is a coefficient which measures the quality of the separation in the result of a chromatography. The CRF concept have been ...
Gas chromatography[edit]. Further information: Gas chromatography. Gas chromatography (GC), also sometimes known as gas-liquid ... Affinity chromatography[edit]. Further information: Affinity chromatography. Affinity chromatography[15] is based on selective ... Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and ... Reversed-phase chromatography[edit]. Main article: Reversed-phase chromatography. Reversed-phase chromatography (RPC) is any ...
Size exclusion chromatography. Micellar liquid chromatography. Ion chromatography (or ion-exchange chromatography) separates ... Anion exchange chromatography retains anions using positively charged functional group: R-X. +. A. −. +. M. +. B. −. ⇄. R-X. + ... or in chromatography columns. Thin layer chromatography or column chromatography share similarities in that they both act ... This type of chromatography is further subdivided into cation exchange chromatography and anion-exchange chromatography. ...
There are several different types of chromatography including paper and thin layer chromatography (TLC). Chromatography is a ... PAPER CHROMATOGRAPHY. Introduced in 1944, paper chromatography is a technique that involves separation and identification of ... In ASCENDING CHROMATOGRAPHY (the type we will be using), the solvent is in a pool at the bottom of a chamber or jar in which ... Chromatography chamber (large glass jar); can also be a glass, a wide mouth test tube or a section of a 16 or 24 oz. plastic ...
The principle of immunoaffinity or immunoadsorption chromatography is based on the highly specific interaction of an antigen ... with its antibody (1). Immunoaffinity chromatography is a specialized form... ... Immunoaffinity chromatography is a specialized form of affinity chromatography and, as such, utilizes an antibody or antibody ... Phillips, T. M. (1989). High-performance immunoaffinity chromatography. Adv. Chromatogr. 29, 133-173.PubMedGoogle Scholar ...
Chromatography is a way of separating two or more chemical compounds. Chemical compounds are chemicals that are bonded together ... These include gas, high pressure liquid, or ion exchange chromatography.. In general, chromatography uses the differences in ... Chromatography is a way of separating two or more chemical compounds. Chemical compounds are chemicals that are bonded together ...
... chromatography: Subsequent developments: A technique exhibiting great selectivity, affinity chromatography, was first described ... In chromatography: Retention mechanism. Affinity chromatography exploits this feature by binding a ligand with the desired ... In chromatography: Subsequent developments. A technique exhibiting great selectivity, affinity chromatography, was first ... interactive capability to a support such as a gel used in gel-filtration chromatography. The ligand retards a solute with the ...
... is used for water chemistry analysis. Ion chromatographs are able to measure concentrations of major anions ... Created by Monica Z. Bruckner, Montana State University What is Ion Chromatography? ... Ion Chromatography. Created by Monica Z. Bruckner, Montana State University. What is Ion Chromatography?. Ion chromatography is ... How Does Ion Chromatography Work?. Ion chromatography, a form of liquid chromatography, measures concentrations of ionic ...
As a chromatography consumable manufacturer and a contract manufacturing organization (CMO), Regis has an onsite team of ... If chiral separation by supercritical fluid chromatography is best, Regis will handle the project in its state of the art SFC ... This valuable addition to its core expertise in chiral chromatography provides a fast and effective advantage that can tackle ... RegisSEP™ Chiral Separation Service uses supercritical fluid chromatography, a method that uses carbon dioxide for a faster and ...
The present communication deals with the application of paper partition chromatography to the identification of natural ... APART from an early attempt by other authors1-3 to adapt a method of paper chromatography for the examination of natural ... APART from an early attempt by other authors1-3 to adapt a method of paper chromatography for the examination of natural ... The present communication deals with the application of paper partition chromatography to the identification of natural ...
Immobilized artificial membrane chromatography: supports composed of membrane lipids. Anal. Biochem. 176: 36-47.PubMedCrossRef ... Chae WG., Luo C., Rhee D.M., Lombardo C.R., Low P., Pidgeon C. (1991) Immobilized Artificial Membrane Chromatography. In: ... Immobilized Artificial Membrane Chromatography. Initial Studies Using Monomyristoylphosphatidylcholine as a Detergent for ... Use of high-performance size exclusion chromatography to determine the extent of detergent solubilization of human erythrocyte ...
... chromatography: Development chromatography: In terms of operation, in development chromatography the mobile phase flow is ... In chromatography: Development chromatography. In terms of operation, in development chromatography the mobile phase flow is ... In elution chromatography successive samples of the effluent are collected in tubes held in a mechanically driven rotating tray ... In chromatography: Sample recovery. Sample recovery from development chromatograms has been described-that is, detection ...
... the different pigments present in a leaf are separated using paper chromatography. ... This experiment works very well providing care is taken over preparing the spot on the chromatography paper. It should be as ... d On a strip of chromatography paper, draw a pencil line 3 cm from the bottom.. ... i Avoid moving the beaker in any way once the chromatography has started.. ...
Find current solutions to practical chromatographic separations and learn how to optimize chromatography processes for the best ... conference focusing on the most cutting-edge areas of preparative and process chromatography. ... Preparative and Process Chromatography. Most recognized international conference in the world for the presentation and ... Keynote Session on Industrial Case Studies in Protein Chromatography - Co-chairs: A. Hunter, T. Pabst (MedImmune) ...
These include hydrophobic interaction chromatography, reversed phase chromatography, two-dimensional chromatography and many ... Ion exchange chromatography. One of the most popular methods for separating proteins is ion exchange chromatography. It ... Affinity chromatography. Affinity chromatography is based on binding interactions between a protein and a ligand immobilized to ... Size exclusion chromatography. Related Stories. *High-performance porous polymeric material for chromatography applications ...
There are a variety of uses for affinity chromatography purification techniques. They are being increasingly used to separate ... Principles of Chromatography. Play. Lectin Affinity Chromatography. This form of chromatography is growing in use. Lectins are ... Immobilized Metal Ion Affinity Chromatography. Immobilized metal ion affinity chromatography (IMAC) involves binding with ... www.sigmaaldrich.com/life-science/proteomics/protein-chromatography/affinity-chromatography.html#applications ...
Title: IONIC CHROMATOGRAPHY STANDARDS, Author: Stephane Schein, Name: IONIC CHROMATOGRAPHY STANDARDS, Length: 29 pages, Page: 1 ... IONIC CHROMATOGRAPHY STANDARDS. BARIUM MONO-ELEMENT STANDARD FOR IONIC CHROMATOGRAPHY Element: Baryum - Ba2+ Ref.: A 005 100 ... IONIC CHROMATOGRAPHY STANDARDS CONCENTRATED ELUENTS FOR IONIC CHROMATOGRAPHY Solution: 0,18M Carb./ 0,17M Bicarb. Ref.: A 105 ... IONIC CHROMATOGRAPHY STANDARDS MONO-ELEMENT STANDARD FOR IONIC CHROMATOGRAPHY Element: Succinate - OOC(CH2)2COO2Ref.: A 044 100 ...
These look the same on paper but have different chromatography results. ... Outreach: crime scene chromatography Description. The best pens for this activity are (1) A black felt tip pen (2) A black ... Outreach: chromatography, how black is a black pen? Experiment with the Vikings FunKids Radio: Kareenas Chemistry Outreach: ... These look the same on paper but have different chromatography results. The best pens for this activity are (1) A black felt ...
... create a rainbow of colors while exploring capillary action and chromatography. ... create a rainbow of colors while exploring capillary action and chromatography. ...
Readers will discover how DNA chromatography can be used in their own work. Intended as an introduction for ... ... DNA chromatography. [Douglas T Gjerde; Christopher P Hanna; D Hornby; John Wiley & Sons.] -- This book describes a powerful and ... Chromatography, High Pressure Liquid. a schema:Intangible ;. schema:name "Chromatography, High Pressure Liquid"@en ;. .. ... DNA chromatography. Author:. Douglas T Gjerde; Christopher P Hanna; D Hornby; John Wiley & Sons.. ...
... column chromatography) or a strip of filter paper (paper chromatography) or by a gel. See more. ... Chromatography definition, the separation of mixtures into their constituents by preferential adsorption by a solid, as a ... chromatography. Historical Examples. of chromatography. *. Mr Field, in his Chromatography, has rendered a very great service ... Origin of chromatography. First recorded in 1725-35; chromato- + -graphy. Related formschro·ma·tog·ra·pher, nounchro·mat·o· ...
Chromatography Standards. Certified Reference Materials & reference standards for chromatography-based testing applications ... Thin Layer Chromatography (TLC). We offer a variety of TLC plates including classical TLC plates, HPTLC plates, MS-Grade TLC ... Browse our Gas Chromatography (GC) units, columns, consumables, and accessories for your analytical GC applications to achieve ... The selection of a buffer in terms of species, ionic strength and pH range is critical in reversed-phase chromatography. Learn ...
GE Healthcare Blue Sepharose™ 6 Fast Flow Chromatography Media Blue dye binds to proteins such as albumin and lipoproteins as ... GE Healthcare Capto™ Multimodal (MMC) Chromatography Media Used to solve specific purification challenges at both high and low ... GE Healthcare MabSelect Sure™ LX Chromatography Media Protein A affinity medium with high dynamic binding capacity which ... GE Healthcare Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media USe for separations of biomolecules with an affinity ...
... to form a fluid tight seal between the polymer cap and container suitable for use in low pressure liquid chromatography. ... Column for liquid chromatography. US4758340. 19 Oct 1987. 19 Jul 1988. Labomatic Ag. Sealing device for a chromatography column ... Chromatography column. US5601708. 14 Sep 1995. 11 Feb 1997. Dyax Corp.. Apparatus for pressurizing a removable chromatography ... Multipart chromatography column connection. US5238556. 13 May 1992. 24 Aug 1993. Hamid Shirkhan. Chromatography tube for use ...
ANION CHROMATOGRAPHY. Scope and Conditions for Separation. Suppressed Anion Chromatography. Nonsuppressed Ion Chromatography. ... Applications of DNA Chromatography. Applications of RNA Chromatography. SAMPLE PRETREATMENT. Dilute and Shoot or Pre-Treat the ... DNA AND RNA CHROMATOGRAPHY. Introduction. DNA and RNA Chemical Structure and Properties. DNA and RNA Chromatography. ... Chelating Ion-Exchange Resins and Chelation Ion Chromatography. ION-EXCLUSION CHROMATOGRAPHY. Principles. Separation of Organic ...
Purchase Affinity Chromatography and Biological Recognition - 1st Edition. Print Book & E-Book. ISBN 9780121665807, ... Affinity Chromatography of Interferons-A Novel Application for Calmodulin-Sepharose. Dextran-Sepharose Affinity Chromatography ... Subunit Exchange Chromatography: Principles and Applications. Affinity Chromatography Studies of Cooperative Interactions in ... IMA-Chromatography (Immobilized Ion Affinity Chromatography): Reflections of Methodological Development. Preparative and ...
Does the red in Skittles look the same as the red in M&Ms when you test both using paper chromatography? What about their other ... Candy Chromatography: What Makes Those Colors?, from Science Buddies. This activity brought to you in partnership with Science ... In each chromatography setup there is generally a so-called mobile element (a fluid in which the components are dissolved) and ... In paper chromatography different pigments can be separated based on their solubility, or their ability to dissolve in water. ...
To reduce chromatography complexity and conserve costly Protein A, CMC Biologics evaluated multicolumn solutions and selected ... Multicolumn Chromatography. While the concept of multicolumn chromatography-also referred to as simulated moving bed (SMB) ... Protein A capture chromatography is a crucial step in all downstream processing of mAbs. The process provides a highly ... In application, multicolumn chromatography allows for product breakthrough in the load zone of the first column, with the ...
PRNewswire/ -- According to a new market research report Gas Chromatography Market by Instrument (Systems, Detectors), ... Browse 86 market data Tables and 31 Figures spread through 167 Pages and in-depth TOC on Gas Chromatography Market ... According to a new market research report Gas Chromatography Market by Instrument (Systems, Detectors), Accessories and ... Chromatography Instruments Market by System (LC (HPLC, UHPLC, Flash), GC, Other Components (Auto samplers, Detectors, Fraction ...
... Liquid chromatography (LC) testing and analysis.. Intertek provides Liquid Chromatography (LC) ... Liquid chromatography allows for selective and highly sensitive detection of trace and molecular-species specific compounds. ...
... Anthea Scothern anthea.scothern at umist.ac.uk Wed Dec 13 04:37:21 EST 2000 *Previous message: ...
  • paper chromatography , gas chromatography , and what would become known as high-performance liquid chromatography . (wikipedia.org)
  • Introduced in 1944 , paper chromatography is a technique that involves separation and identification of chemical substances by a solvent that is moving up the sheets or strips of filter paper. (angelfire.com)
  • APART from an early attempt by other authors 1-3 to adapt a method of paper chromatography for the examination of natural saponins, the potentialities in this field do not seem to have been investigated. (nature.com)
  • In this experiment, the different pigments present in a leaf are separated using paper chromatography. (rsc.org)
  • the separation of mixtures into their constituents by preferential adsorption by a solid, as a column of silica (column chromatography) or a strip of filter paper (paper chromatography) or by a gel. (dictionary.com)
  • This same process can be put to good use as a technique called paper chromatography. (scientificamerican.com)
  • For example, in paper chromatography water is the mobile phase and paper is the stationary phase. (scientificamerican.com)
  • In paper chromatography different pigments can be separated based on their solubility, or their ability to dissolve in water. (scientificamerican.com)
  • A description of how the techniques developed, starting from paper chromatography up-to current 'high tech' two-dimensional separations. (brighttalk.com)
  • Paper chromatography is a technique for separating and identifying mixtures that are (or can be) coloured. (wikipedia.org)
  • Double-way paper chromatography , also called 'two-dimensional chromatography', uses two solvents and rotates the paper 90° in between. (wikipedia.org)
  • Thin layer chromatography (TLCC) is a common laboratory technique similar to paper chromatography. (wikipedia.org)
  • For paper chromatography, dyes are marked out on a designated type of paper and a solvent (coffee filters and water in our case). (dmns.org)
  • What factors play a role in separating pigments with paper chromatography? (reference.com)
  • When using paper chromatography, the solubility of the pigments, how attractive the paper is and the size of the particles influence the separation of pigm. (reference.com)
  • To apply the technique of paper chromatography as a method of separating individual plant pigments contained in plant tissue extracts containing pigment blends. (prezi.com)
  • The pigments can be identified using paper chromatography method. (prezi.com)
  • Paper chromatography is a useful technique in the separation and identification of different plant pigment. (prezi.com)
  • They established the principles and basic techniques of partition chromatography, and their work encouraged the rapid development of several chromatographic methods: paper chromatography, gas chromatography, and what would become known as high-performance liquid chromatography. (wikipedia.org)
  • The purification method can also be combined with other types of analysis equipment such as high-erformance liquid chromatography (HPLC) and mass spectrometry. (news-medical.net)
  • Currently four systems are available to carry out liquid chromatography, two of these systems are also set up for preparative HPLC. (york.ac.uk)
  • Enantiomeric separation ability on high-performance liquid chromatography (HPLC) was compared between native and acetylated β-cyclodextrin stationary phases. (go.jp)
  • High-pressure liquid chromatography, also called high performance liquid chromatography (HPLC), is now widely used in industry. (encyclopedia.com)
  • A variation on HPLC is super-critical fluid chromatography (SFC). (encyclopedia.com)
  • The Beginners Guide to Liquid Chromatography , a 56-page paperback book, is a useful tool for those learning about the technology of liquid chromatography (LC), with a focus on high-performance liquid chromatography (HPLC). (wiley.com)
  • U.S. Geological Survey (USGS) scientist using a high-performance liquid chromatography (HPLC) system to fractionate water samples for identification of biologically-active chemicals. (usgs.gov)
  • High- performance liquid chromatography (HPLC) is also sometimes referred to as high- pressure liquid chromatography. (wikipedia.org)
  • One of the advanced forms of liquid chromatography is the high-performance liquid chromatography (HPLC) for ultra-trace separations with high sensitivity and accuracy, thereby augmenting the market growth. (openpr.com)
  • Preparation of pure substance for clinical, toxicology studies, inorganic studies, and trace analysis make high-performance liquid chromatography (HPLC) the most opted technique. (openpr.com)
  • Originally it has been developed with the application of HPLC, and recently herbal samples' biological profiles have been obtained by means of thin-layer chromatography (TLC). (hindawi.com)
  • Several chromatographic methods have been applied for fingerprint construction, namely, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), gas chromatography (GC), or high-speed counter current chromatography (HSCCC). (hindawi.com)
  • Written by the author of "HPLC: A Practical Guide" (RSC, 1999), the book examines not only the behaviour of biological reactions per se, but also describes the behaviour of biological molecules in chromatography systems. (rsc.org)
  • Clarity Controls provides an interface with chromatography devices, such as GC and HPLC systems, Autosamplers and Valves. (scientific-computing.com)
  • There are several different types of chromatography including paper and thin layer chromatography (TLC). (angelfire.com)
  • A somewhat different approach is the set of techniques known as "planar" or "thin layer" chromatography (TLC), in which no column is used at all. (encyclopedia.com)
  • There are also terms like "Retention factor value" meaning the distance run by a component spot, divided by the distance run by the solvent in thin layer chromatography. (bartleby.com)
  • The TLC SpotON is a semi-automated device used to apply samples on Thin Layer Chromatography plates. (environmental-expert.com)
  • Toluene, ethyl formate, chloroform (CH[Cl.sub.3]), ethyl acetate (EtOAc), methanol (MeOH), ethanol (EtOH), distilled water, and formic acid were used as the open column chromatography and thin layer chromatography. (thefreedictionary.com)
  • It has been largely replaced by thin layer chromatography , but is still a powerful teaching tool. (wikipedia.org)
  • Chromatography is a laboratory technique for the separation of a mixture. (wikipedia.org)
  • [4] He developed the technique, he coined chromatography, in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll , carotenes , and xanthophylls . (wikipedia.org)
  • New types of chromatography developed during the 1930s and 1940s made the technique useful for many separation processes . (wikipedia.org)
  • Advances are continually improving the technical performance of chromatography, allowing the separation of increasingly similar molecules. (wikipedia.org)
  • Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. (wikipedia.org)
  • Starting from 1947, Spedding and Powell used displacement ion-exchange chromatography for the separation of the rare earths. (wikipedia.org)
  • At the start of the 1950s, Kraus and Nelson demonstrated the use of many analytical methods for metal ions dependent on their separation of their chloride, fluoride, nitrate or sulfate complexes by anion chromatography. (wikipedia.org)
  • Regis Technologies offers a chiral chromatography service for the separation and purification of chiral compounds. (prweb.com)
  • RegisSEP™ Chiral Separation Service uses supercritical fluid chromatography, a method that uses carbon dioxide for a faster and more cost-effective solution. (prweb.com)
  • Regis's RegisSEP™ Chiral Separation Service uses supercritical fluid chromatography (SFC). (prweb.com)
  • If chiral separation by supercritical fluid chromatography is best, Regis' will handle the project in its state of the art SFC Purification Lab, which is part of a 30,000 square foot cGMP facility regularly inspected by the FDA. (prweb.com)
  • Contact Regis or meet their team at SFC 2015 in Philadelphia July 22nd-24th for more information on Supercritical Fluid Chromatography and the RegisSEP Chiral Separation Service. (prweb.com)
  • The present communication deals with the application of paper partition chromatography to the identification of natural saponins and the separation of the constituents present, with particular reference to saponins of the important medical plants. (nature.com)
  • Four days of exciting science, technology, and education at PREP 2019, the longest running, most recognized international scientific conference and exposition driving the field of Preparative and Process Chromatography, organized by expert scientists and engineers for the separation science practitioner. (archive.org)
  • For people seeking comprehensive coverage of bio-chromatography from process scale to analytics, a single, discounted registration fee will provide access to both the PREP 2019 and the ISPPP 2019 Conference on the separation, purification and characterization of biologically important molecules that will be held the same week in the same conference venue. (archive.org)
  • Chromatography is a technique that is used for separation of mixtures. (news-medical.net)
  • In modern chromatographic separations, the stationary phase comes in the form of media, either pre-packed in a chromatography column or added by the user to the separation apparatus. (news-medical.net)
  • These include hydrophobic interaction chromatography, reversed phase chromatography, two-dimensional chromatography and many others that are used to explore and achieve the perfect separation. (news-medical.net)
  • Ion chromatography (IC) laboratories support analytical testing projects requiring separation and identification of components and trace materials. (intertek.com)
  • Intertek IC separation and chromatography lab testing capabilities are available throughout the global laboratory network. (intertek.com)
  • Fabrication of a monolithic polymer layer, as the stationary phase, equipped with two electrodes was studied for simultaneous two-dimensional separation, combining high performance liquid chromatography and orthogonal electrophoresis. (go.jp)
  • The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. (springer.com)
  • Simulated moving bed chromatography (SMBC) is increasingly applied as a separation technique in the pharmaceutical industry, production of fine chemicals and in the field of bioengineering. (environmental-expert.com)
  • In the mid-1970s, interest in liquid mobile phases for column chromatography resurfaced when it was discovered that the efficiency of separation could be vastly improved by pumping the liquid through a short packed column under pressure, rather than allowing it to flow slowly down a vertical column by gravity alone. (encyclopedia.com)
  • The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. (elsevier.com)
  • The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science . (elsevier.com)
  • Journal of Chromatography A welcomes the submission of research papers which report on studies concerning the development of new and significant advances in separation science. (elsevier.com)
  • Column chromatography is a common and useful separation technique in organic chemistry and can be used in both large and small scale. (thefreedictionary.com)
  • CHROMATOGRAPHY Chromatography was originally developed in the year 1903 by the Russian botanist Michael Tswett in percolating a petroleum ether extract through a glass column packed with powered calcium carbonate for the separation of colored pigments. (ipl.org)
  • Planar Chromatography-Mass Spectrometry focuses on a relatively new approach to chemical analysis in general, and to separation science in particular. (routledge.com)
  • This book overviews the combination of planar chromatography, a relatively simple and cost-effective separation step for determining complex mixtures of compounds, with mass spectrometry, an efficient, highly instrumental, and relatively expensive technique that enables rapid identification of separated chemical species. (routledge.com)
  • Aiming to improve power and efficiency when other analytical methods are inadequate, Planar Chromatography-Mass Spectrometry encourages separation science practitioners in academia and industry to combine the two methods for enhanced results. (routledge.com)
  • This is fueling the demand for separation services and gas chromatography in this region. (pitchengine.com)
  • Reverse phase liquid chromatography (RPLC) is a commonly used separation and analysis technique. (mdpi.com)
  • In order to simulate the separation process in liquid chromatography the competitive adsorption isotherms need to be known. (diva-portal.org)
  • The most up-to-date compilation of significant research on preparative liquid chromatography used for the separation of biomolecules and proteins. (valorebooks.com)
  • The dissolved cerebroside which was obtained by using chloroform and methanol (2:1/v:v) was subjected to column chromatography on silica gel, and then was eluted with chloroform-methanol-H2O (20:1:0.2), by which 3 fractions (1,2,3) was afforded. (thefreedictionary.com)
  • Column chromatography was performed using silica gel 60 (Merck, 70-230 mesh). (thefreedictionary.com)
  • When compared to classical column chromatography high performance liquid chromatography is so called because of its improved performance. (ipl.org)
  • Column chromatography separates compounds using many chemical actions between the chemical being tested and the chromatography column (a rod with a blending of special chemicals). (wikipedia.org)
  • The most common stationary phase for column chromatography is silica gel, followed by alumina . (wikipedia.org)
  • It is column chromatography run under pressure to raise the speed of the process. (wikipedia.org)
  • Ion chromatography (or ion-exchange chromatography ) separates ions and polar molecules based on their affinity to the ion exchanger. (wikipedia.org)
  • Cation-exchange chromatography is used when the molecule of interest is positively charged. (wikipedia.org)
  • Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it. (wikipedia.org)
  • Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. (wikipedia.org)
  • [8] For example, when cation exchange chromatography is used, cations will elute out last. (wikipedia.org)
  • However, there are also disadvantages involved when performing ion-exchange chromatography, such as constant evolution with the technique which leads to the inconsistency from column to column. (wikipedia.org)
  • The boom of Ion exchange chromatography primarily began between 1935-1950 during World War II and it was through the " Manhattan project " that applications and IC were significantly extended. (wikipedia.org)
  • These include gas, high pressure liquid, or ion exchange chromatography. (medlineplus.gov)
  • One of the most popular methods for separating proteins is ion exchange chromatography. (news-medical.net)
  • If a negatively charged resin is used to capture positively charged molecules, the method is referred to as cation exchange chromatography. (news-medical.net)
  • The opposite, in which the resin is positively charged and the target molecule is negatively charged, is called anion exchange chromatography. (news-medical.net)
  • SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. (fishersci.com)
  • This book presents a systematic and comprehensive review of the information on chromatographic processes that involve the formation of coordination compounds, aiming not only to demonstrate the achievements that have been made in the theory of praxis of chromatography, but also to point out, as far as possible, the future of potential of ligand exchange chromatography. (routledge.com)
  • The Journal of Planar Chromatography , dedicated to all aspects of this chromatographic technique, is a peer reviewed journal published in English. (springer.com)
  • Empower Software , our flagship chromatography data system (CDS), makes it easier than ever to run samples and produce meaningful results without experiencing downtime for training, re-engineering workflow, or adding new software to support instruments or advanced chromatographic techniques. (waters.com)
  • Upfront Chromatography is the leading global provider of industrial scale chromatographic extraction technology. (fiercebiotech.com)
  • The gas chromatography market has seen an extraordinary amount of technological advancements in the last few years, as companies have introduced new systems that perform chromatographic procedures in a lesser amount of time and provide more detailed results. (pitchengine.com)
  • Various secondary sources such as associations like the Chromatographic Society (ChromSoc), Chinese American Chromatography Association (CACA), Chromatographic Society of India, directories, industry journals, databases, and annual reports of the companies have been used to identify and collect information useful for the study of this market. (marketsandmarkets.com)
  • Clarity Chromatography Software has a strong position in chromatographic data systems. (scientific-computing.com)
  • Regis Technologies, Inc. is a recognized leader in developing and manufacturing of chiral stationary phases (CSPs) for analytical and preparative chromatography separations. (prweb.com)
  • This valuable addition to its core expertise in chiral chromatography provides a fast and effective advantage that can tackle projects for the scale clients need-from milligram to kilogram scale projects for non-GMP and GMP separations. (prweb.com)
  • As a chromatography consumable manufacturer and a contract manufacturing organization (CMO), Regis has an onsite team of experts in separations, scale up, synthesis, and quality that can support any project's needs. (prweb.com)
  • Intertek provides Liquid Chromatography (LC) and separations laboratory capabilities for advanced and routine analysis. (intertek.com)
  • This type of chromatography is ideal for separations that need to take place in a fast paced manner. (buzzfeed.com)
  • The United States Supercritical Fluid Chromatography (Sfc) Industry Market Research Report 2017 is a professional and in-depth study on the current state of the Supercritical Fluid Chromatography (Sfc) industry. (mynewsdesk.com)
  • The 2017/18 Waters Quality Parts, Chromatography Columns and Supplies Catalog is available! (waters.com)
  • The global Gel Permeation Chromatography market 2017 is also analysed on the basis of product pricing, Gel Permeation Chromatography production volume, data regarding demand and Gel Permeation Chromatography supply, and the revenue garnered by the product. (openpr.com)
  • The global chromatography instruments market is projected to be valued at USD 7.86 Billion in 2017 and is expected to grow at a CAGR of 6.9% to reach to USD 10.99 Billion by 2022. (marketsandmarkets.com)
  • 2017. "Subcritical Water Chromatography with Electrochemical Detection. (mdpi.com)
  • For more information about other types of chromatography, please visit the National Sciences Digital Library Collection . (carleton.edu)
  • In liquid chromatography: Charged aerosol detector (CAD) Evaporative light scattering detector (ELSD) In gas chromatography: Flame ionization detector (FID) Flame photometric detector (FPD) Nitrogen Phosphorus Detector (NPD) Atomic-emission detector (AED) In all types of chromatography: Mass spectrometer (MS) In liquid chromatography: UV detectors, fixed or variable wavelength, which includes diode array detector (DAD or PDA). (wikipedia.org)
  • Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won the 1952 Nobel Prize in Chemistry . (wikipedia.org)
  • Ion chromatography is used for water chemistry analysis. (carleton.edu)
  • This ion chromatography machine can analyze water chemistry. (carleton.edu)
  • It is the first book to systemically cover the theoretical background, techniques, instrumentation, and practical applications of planar chromatography-mass spectrometry as a hyphenated tool of analytical chemistry. (routledge.com)
  • Chromatography is much used in biochemistry and analytical chemistry . (wikipedia.org)
  • Understand that chromatography is the chemistry of taking things apart through the use of colors. (dmns.org)
  • The molecule is positively charged because the pH for chromatography is less than the pI (a/k/a pH(I)). [2] In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. (wikipedia.org)
  • In the case of chromatography certain molecules will stick to the cellulose of the filter paper more strongly than others. (angelfire.com)
  • Immobilized metal ion affinity chromatography (IMAC) involves binding with target molecules such as proteins, nucleic acids, amino acids and peptides and metal ions, for example Zn2+, Ni2+, Cu2+, and Fe3+ that have been immobilized in a column. (news-medical.net)
  • Chromatography is well-known for analytical purposes but it is also used to purify molecules at industrial scale with applications in the food, chemical and bio-based industries. (environmental-expert.com)
  • Affinity chromatography is used to capture molecules by specific interaction between the chromatography ligand and the target. (sartorius.com)
  • Heparin HyperD™ M composite chromatography sorbent is an affinity preparative sorbent designed for the purification of biological molecules that bind to heparin, such as coagulation factors, growth factors, lipoproteins. (sartorius.com)
  • Liquid chromatography is a technique used to separate and identify proteins, nucleic acid, and small molecules in a mixture. (openpr.com)
  • Gel Permeation Chromatography (GPC) plays an essential role in determination of dispersity and molecular weight of polymers and complex molecules. (4spe.org)
  • 1. Answers to your questions asked in Professor Schoenmakers' Introduction to Liquid Chromatography webinar. (brighttalk.com)
  • After the webinar, you will have a better understanding of how a refractometer can improve your chromatography. (4spe.org)
  • Regis also plans to publish over 100 chiral applications using supercritical fluid chromatography online this summer. (prweb.com)
  • United States Supercritical Fluid Chromatography (Sfc) Industry. (mynewsdesk.com)
  • The Supercritical Fluid Chromatography (Sfc) market analysis is provided for the United States markets including development trends, competitive landscape analysis, and key regions development status. (mynewsdesk.com)
  • The Supercritical Fluid Chromatography (Sfc) industry development trends and marketing channels are analyzed. (mynewsdesk.com)
  • With a paper towel, a black marker, and a cup of water, create a rainbow of colors while exploring capillary action and chromatography. (exploratorium.edu)
  • Besides Ion Chromatography his research interests are titrations, solid-phase extractions, capillary electrophoresis and analytical complexing reagents. (wiley.com)
  • Hyphenations of Capillary Chromatography with Mass Spectrometry provides comprehensive coverage of capillary chromatography with mass spectrometry-both single and multidimensional approaches. (elsevier.com)
  • The book examines nearly all capillary chromatography approaches, combined with a variety of MS forms, giving readers a wide and detailed view on current-day analytical strategies and applications. (elsevier.com)
  • Junior scientists conducting research on mono-dimensional chromatography-MS fundamental relationships and experienced analytical chemists working in conventional capillary chromatography and classical multidimensional chromatography will find this an ideal application-based reference on the hyphenations of these domains. (elsevier.com)
  • Capillary gas chromatography-mass spectro. (mendeley.com)
  • Researchers found that the main principles of Tsvet's chromatography could be applied in many different ways, resulting in the different varieties of chromatography described below. (wikipedia.org)
  • The simplicity of these instruments allows students to grasp the operating principles of gas chromatography without a lot of automation. (kutztown.edu)
  • High Performance Liquid Chromatography: Fundamental Principles and Practice. (wikipedia.org)
  • Join us for a dynamic and innovative program where world renowned speakers will address in-depth the latest scientific and technological advances, critical and emerging applications and processes, and challenges and solutions in all aspects of Preparative and Process Chromatography. (archive.org)
  • During the exhibition, evaluate and compare the latest products, instruments, techniques and processes that are driving the pace of innovation in Preparative and Process chromatography. (archive.org)
  • Transporting the metered liquid sample to the point where it is to be vaporized is a fundamental problem in process chromatography. (google.com)
  • Discusses novel approaches to the preparative/process chromatography of complex carbohydrate and glycoconjugates. (valorebooks.com)
  • The research report analyses the historical as well as present performance of the worldwide Gel Permeation Chromatography industry, and makes predictions on the future status of Gel Permeation Chromatography market on the basis of this analysis. (openpr.com)
  • The report studies the industry for Gel Permeation Chromatography across the globe taking the existing industry chain, the import and export statistics in Gel Permeation Chromatography market & dynamics of demand and supply of Gel Permeation Chromatography into consideration. (openpr.com)
  • The ' Gel Permeation Chromatography ' research study covers each and every aspect of the Gel Permeation Chromatography market globally, which starts from the definition of the Gel Permeation Chromatography industry and develops towards Gel Permeation Chromatography market segmentations. (openpr.com)
  • Further, every segment of the Gel Permeation Chromatography market is classified and analysed on the basis of product types, application, and the end-use industries of the Gel Permeation Chromatography market. (openpr.com)
  • The geographical segmentation of the Gel Permeation Chromatography industry has also been covered at length in this report. (openpr.com)
  • The competitive landscape of the worldwide market for Gel Permeation Chromatography is determined by evaluating the various industry participants, production capacity, Gel Permeation Chromatography market's production chain, and the revenue generated by each manufacturer in the Gel Permeation Chromatography market worldwide. (openpr.com)
  • Various methodical tool of Gel Permeation Chromatography such as investment returns, feasibility, and market attractiveness analysis has been used in Gel Permeation Chromatography market research to present a comprehensive study of the industry for Gel Permeation Chromatography across the globe. (openpr.com)
  • Gel Permeation Chromatography Market Research 2018 Globel Info Research Recently added detailed market study on the "Global Gel Permeation Chromatography Market" Research Report 2018-2025 which provides an outlook of current market value of Gel Permeation Chromatography Market as well as the expected forecast of Rate on Investment (ROI) with growing CAGR of XX% in Gel Permeation Chromatography Market by the end of 2025. (openpr.com)
  • Fragmented Market to provide Opportunities for Small Players Presently, the global gel permeation chromatography market is highly fragmented and competitive owing to the presence of many established players in the market. (openpr.com)
  • The purpose of preparative chromatography is to separate the components of a mixture for later use, and is thus a form of purification . (wikipedia.org)
  • Immunoaffinity chromatography is a specialized form of affinity chromatography and, as such, utilizes an antibody or antibody fragment as a ligand immobilized onto a solid support matrix in a manner that retains its binding capacity. (springer.com)
  • High-performance immunoaffinity chromatography. (springer.com)
  • Hage, D. S. (1998) Survey of recent advances in analytical applications of immunoaffinity chromatography. (springer.com)
  • Katz, S. E. and Brady, M. S. (1990) High-performance immunoaffinity chromatography for drug residue analysis. (springer.com)
  • Katz, S. E. and Siewierski, M. (1992) Drug residue analysis using immunoaffinity chromatography. (springer.com)
  • VanGinkel, L. A. (1991) Immunoaffinity chromatography, its applicability and limitations in multi-residue analysis of anabolizing and doping agents. (springer.com)
  • Jack, G. W. and Wade, H. E. (1987) Immunoaffinity chromatography of clinical products. (springer.com)
  • Cutler P. (2004) Immunoaffinity Chromatography. (springer.com)
  • Growth in this market can be attributed to the increasing crude & shale oil production, increasing importance of waste water treatment, rising adoption of GC-MS, initiatives to reduce environmental pollution levels, growing food safety concerns, and increased importance of chromatography in drug testing. (prnewswire.com)
  • Increased importance of chromatography in drug approvals is likely to drive the growth of this market. (pitchengine.com)
  • For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). (fishersci.com)
  • As a result of a systematic study, we propose the use of an immobilized metal affinity chromatography (IMAC) in a microtip (Erdjument-Bromage, H. (nih.gov)
  • Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. (elsevier.com)
  • Liquid chromatography can be used for analytical and preparative applications such as food supplement and pharmaceutical or bio therapeutic. (openpr.com)
  • Also, several lectin columns can be combined for the purification of glycoproteins and glycoconjugates in an approach called serial lectin affinity chromatography. (news-medical.net)
  • Browse our Gas Chromatography (GC) units, columns, consumables, and accessories for your analytical GC applications to achieve high performance, maximum productivity, and superior detection sensitivity. (sigmaaldrich.com)
  • And because the technology uses several chromatography columns in parallel, it can be leveraged by manufacturers wishing to switch from batch to continuous processing, as multiple steps can be run simultaneously. (genengnews.com)
  • Continuous chromatography enhances the overall column binding capacity by splitting the load zone into two columns in series. (genengnews.com)
  • The columns segment accounted for the largest share of the global Gas Chromatography Market in 2016. (prnewswire.com)
  • The large share of this segment can primarily be attributed to factors such as the availability of improved gas chromatography columns for the petroleum industry and the discovery of new oil fields. (prnewswire.com)
  • Phenomenex has opened a manufacturing and development facility to produce its gas chromatography (GC) columns. (foodnavigator.com)
  • Agilent Technologies has introduced InfinityLab - a product family of liquid chromatography instruments, columns and supplies - at Analytica 2016 in Munich. (foodnavigator.com)
  • IO 3 - , NO 3 - , I - ) on the prepared columns was investigated by ion chromatography, and was then compared to the retention behavior using stationary phases reacted with other amines. (go.jp)
  • Bio chromatography columns offer a single and unique packing method with very high robustness. (environmental-expert.com)
  • The report "Gas Chromatography Market by Instrumentation (Systems, Detectors, Autosamplers, Fraction collectors), by Consumables & Accessories (Columns, Fittings & Tubings, Flow Management), by Reagents (Analytical, Preparative) - Analysis & Global forecast to 2019" , this report studies the global gas chromatography market over the forecast period of 2014 to 2019. (pitchengine.com)
  • Affinity chromatography is frequently used for downstream purification of proteins & vaccines. (sartorius.com)
  • The present invention relates to the purification of proteins, and, more particularly, to the purification of clotting Factor VIII:C by means of affinity chromatography. (freepatentsonline.com)
  • Students use chromatography to separate the pigments in a leaf. (rsc.org)
  • Hi for biology we had to do a lab which we separated the plant pigments by chromatography of spinach. (physicsforums.com)
  • Chromatography is used to study plant pigment by extracting pigments from the plant and then sorting them by their physical characteristics so they can be analyzed. (reference.com)
  • Chromatography is useful in separating different types of plant pigments because they are very similar to one another and can be difficult to distinguish or physically separate otherwise. (reference.com)
  • Birzeit University's Testing Laboratories Center (TLC), in cooperation with Techno-Line Medical and Lab Equipment―Palestine, reviewed the latest developments in chemical analysis in a workshop titled "Liquid chromatography MS platforms and cGMP" in a lecture held on October 30, 2019. (birzeit.edu)
  • Affinity chromatography exploits this feature by binding a ligand with the desired interactive capability to a support such as a gel used in gel-filtration chromatography. (britannica.com)
  • Affinity chromatography is based on binding interactions between a protein and a ligand immobilized to a resin. (news-medical.net)
  • Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. (springer.com)
  • Chromatography, a term that literally means "colored writing or picture", is a technique ordinarily used to separate a series of solutes mixed within a common solvent. (angelfire.com)
  • In ASCENDING CHROMATOGRAPHY (the type we will be using), the solvent is in a pool at the bottom of a chamber or jar in which the paper is supported. (angelfire.com)
  • 7. After that, hang the chromatography strip to the stopper using a pin/clip(pointed end down into the solvent). (prezi.com)
  • 10. Take out the chromatography strip when the solvent front comes close to the top. (prezi.com)
  • Eluent (sometimes spelled eluant) - the solvent or solvent mixure used in elution chromatography and is synonymous with mobile phase. (wikipedia.org)
  • This schematic diagram of an ion chromatography run depicts how elution time correlates to output peak data. (carleton.edu)
  • While ion exchange, size exclusion and affinity chromatography media are some of the most popular and commonly used options, there are dozens of other methods. (news-medical.net)
  • GE Healthcare Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes. (fishersci.com)
  • Entitled 'Atlas CDS-A scalable, compliant and integrated chromatography data system,' this document is available to chromatographers working in both regulated and non-regulated industries to address the benefits of standardizing on a single solution. (mdtmag.com)
  • There are different phases with chromatography: Stationary (solid or liquid supported by a solid) and Mobile (liquid or gas). (dmns.org)
  • Chromatography is a way of separating two or more chemical compounds. (medlineplus.gov)
  • Liquid chromatography allows for selective and highly sensitive detection of trace and molecular-species specific compounds. (intertek.com)
  • Chromatography is a family of laboratory techniques for separating mixtures of chemicals into their individual compounds. (encyclopedia.com)
  • The basic principle of chromatography is that different compounds will stick to a solid surface or dissolve in a film of liquid to different degrees. (encyclopedia.com)
  • Because it usually does not alter the molecular structure of the compounds, chromatography can provide a non-destructive way to obtain pure chemicals from various sources. (encyclopedia.com)
  • Chromatography has evolved over the years and become a method of choice for analysis, identification, and purification of a wide variety of compounds. (pitchengine.com)
  • The volatile compounds in Roselle extract and dried samples were performed using needles of solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS HP-5890). (scribd.com)
  • Gas chromatography-olfactometry (GC-O), also known as "sniffing", is highly useful for establishing aroma profiles as it allows the discrimination of odor-active compounds in complex matrices [ 9 - 14 ]. (hindawi.com)
  • Visit the Chiral Applications page today to search hundreds of applications using normal and reverse phase chromatography. (prweb.com)
  • The complexity of traditional chromatography options, as well as the high cost of the Protein A resin required, have driven the search for a more flexible, cost-effective, and convenient alternative. (genengnews.com)
  • Also describes recent advances in column materials.Horvath, Csaba is the author of 'Chromatography in Biotechnology' with ISBN 9780841226692 and ISBN 0841226695. (valorebooks.com)
  • In these methods, boronate or phenyl borate can be used as affinity ligands in combination with agarose or high-performing affinity chromatography (HPAC) methods for the stationary phase. (news-medical.net)
  • Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity. (springer.com)
  • Doping control analysis predominantly utilises chromatography and mass spectrometry-based approaches to detect prohibited substances and methods of doping. (brighttalk.com)
  • Your primary role is to participate in the chromatography lab in support of the gas and petroleum hydrocarbon characterization team, develop analytical methods, mentor young scientists, and interact with proponents to solve their analytical and operational challenges through the available resources. (rigzone.com)
  • This comprehensive collection of detailed affinity chromatography methods provides all the information necessary to use these powerful techniques successfully in the laboratory and in real world situations. (worldcat.org)
  • Among all the chromatography methods being used to separate different components of any given mixture, flash chromatography is highly popular. (buzzfeed.com)
  • Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. (elsevier.com)
  • The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation , and detection methods such as mass spectrometry . (elsevier.com)
  • The first true chromatography is usually attributed to the Russian-Italian botanist Mikhail Tsvet.Tsvet applied his observations with filter paper extraction to the new methods of column fractionation that had been developed in the 1980s for separating the components of petroleum. (prezi.com)
  • Gas chromatography mass spectrometry-based metabolite profiling in plants. (nih.gov)
  • Corrigendum: Gas chromatography mass spectrometry-based metabolite profiling in plants. (nih.gov)
  • In general, chromatography is a term that describes techniques used to separate components of mixtures. (kutztown.edu)
  • Gas chromatography is a widely-used technique that has been applied to separating, identifying and quantifying components of complex mixtures across a diverse range of disciplines. (kutztown.edu)
  • Agilent Technologies has opened a $14.7m technology centerin Folsom, California to expand capabilities for chromatography consumables and hardware. (foodnavigator.com)
  • Agilent Technologies Inc. introduces the new Hydrogen Sensor for the 7890B Gas Chromatography system, which helps GC users move away from costly helium. (manufacturing.net)
  • Agilent Technologies Inc. introduced the new Hydrogen Sensor for the 7890B Gas Chromatography system. (manufacturing.net)
  • Major companies operating in the manufacturing of liquid chromatography include A.C. Analytical Controls B.V, Thermo Fisher Scientific Inc., Waters Corporation, Agilent Technologies, GL Sciences Inc., PerkinElmer Inc., Shimadzu Corporation, Waters Corporation, and Phenomenex Inc. Major players have a well-established presence in over 50 economies with well-developed and channelized sales, which is expected to make them more competitive in the liquid chromatography market in near future. (openpr.com)
  • The major players in the chromatography instruments market include Agilent Technologies, Inc. (U.S.), Waters Corporation (U.S.), Shimadzu Corporation (Japan), Thermo Fisher Scientific Inc. (U.S.), and PerkinElmer Inc. (U.S. (marketsandmarkets.com)
  • The two types of ion chromatography are anion-exchange and cation-exchange. (wikipedia.org)
  • Following it in 1980, was a similar method for cation chromatography. (wikipedia.org)
  • The market for gas chromatography instrumentation is segmented into systems, detectors, auto-samplers and fraction collectors. (pitchengine.com)
  • Liquid Chromatography Detectors. (wikipedia.org)
  • In size exclusion chromatography (SEC), the sample is filtered through a gel made of spherical beads with specifically-sized pores. (news-medical.net)
  • Often used in size exclusion chromatography for polymer analysis. (wikipedia.org)
  • This form of chromatography is growing in use. (news-medical.net)
  • This form of chromatography works by air pressure driving the mixture, which is to be separated down the vertical glass column. (buzzfeed.com)
  • The activity provides a useful exercise in children applying their previous knowledge of chromatography to help solve the 'crime scene' problem you have set. (rsc.org)
  • However, the data collection software associated with this GC is powerful, and provides all the necessary tools to transfer knowledge of chromatography data analysis to more sophisticated systems. (kutztown.edu)
  • The Gas Chromatography Market is segmented on the basis of instruments, accessories and consumables, end users, and regions. (prnewswire.com)
  • Ion chromatography is a form of liquid chromatography, using ion-exchange resins to separate atomic and molecular ions for analysis. (intertek.com)
  • We can develop customized products to suit your specific needs, including custom bonding of Grace's or your chromatography resins. (grace.com)
  • 1988) High performance liquid chromatography immunoaffinity purification of antibodies and antibody fragments. (springer.com)
  • Downstream bioprocessing steps have long been a challenge to drug manufacturers, particularly the chromatography step for purification of monoclonal antibodies (mAbs). (genengnews.com)
  • The systems segment accounted for the largest share of the global Gas Chromatography Market in 2016. (prnewswire.com)
  • In 2016, the oil and gas industry segment commanded the largest share of the global Gas Chromatography Market. (prnewswire.com)
  • Reviews and papers for Practice of Planar Chromatography are written at the invitation of an Editor. (springer.com)
  • It also examines the high and as-yet unexploited potential of planar chromatography-mass spectrometry for analytical use in scientific investigations. (routledge.com)
  • The book advocates growth in using planar chromatography-mass spectrometry in laboratories that have appropriate equipment but have not yet employed the techniques in combination. (routledge.com)
  • Immobilized artificial membrane chromatography: supports composed of membrane lipids. (springer.com)
  • VYKING™ paramagnetic silica features a novel particle design that clads a paramagnetic core with silica to eliminate the need for chromatography, centrifugation, or vacuum filtration steps required in typical biomolecule purifications. (grace.com)
  • In general, chromatography uses the differences in chemicals to separate them. (medlineplus.gov)
  • While predominantly used in the pharmaceutical area, chiral chromatography has important applications in manufacturing of fine chemicals, agrochemicals, academia, etc. (prweb.com)
  • The mixed substance to be tested is added in a small amount and is slowed by certain chemical or physical activity with the chemicals in the chromatography column. (wikipedia.org)
  • In 1979, a method for anion chromatography with non-suppressed conductivity detection was introduced by Gjerde et al. (wikipedia.org)
  • GE Healthcare Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform. (fishersci.com)
  • For more information about ion chromatography applications, click here . (carleton.edu)
  • Specialist analytical instrument manufacturers in the Schauenburg Analytics Group harness applications and technology expertise to advance analytical performance across the gas chromatography workflow and deliver better business and scientific for research and routine testing laboratories. (environmental-expert.com)
  • Training of technicians and junior scientists in gas chromatography operation and applications. (rigzone.com)
  • What are the applications and advantages/disdvantages of using gas and high pressure chromatography? (physicsforums.com)
  • Chromatography: Applications Pt. (waterstones.com)
  • In gas-liquid chromatography (now called gas chromatography), the material that separates components is chemically bonded to the solid support, which improves the temperature stability of the column's packing. (encyclopedia.com)
  • Ion chromatography, a form of liquid chromatography, measures concentrations of ionic species by separating them based on their interaction with a resin. (carleton.edu)
  • Generally, the material used in chromatography media is a particle or resin packed into a column . (news-medical.net)
  • However, Protein A resin is expensive, which means the costs associated with its use in chromatography can be very high. (genengnews.com)
  • Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture. (wikipedia.org)
  • That certain colours and dyes are made up from a mixture of colours and these can be separated by using chromatography. (rsc.org)
  • In gas chromatography, a gas is used to carry a mixture across a bed of material. (kutztown.edu)
  • A chromatography detector is a device used in gas chromatography (GC) or liquid chromatography (LC) to detect components of the mixture being eluted off the chromatography column. (wikipedia.org)
  • The extent to which any particular component moves up the paper is dependent not only on its solubility in propanone but also on its attraction for the cellulose in the chromatography paper. (rsc.org)
  • A wide range of materials have been used as a solid matrix material for affinity chromatography, including agarose, cellulose, dextran, polyacrylamide, and many more. (news-medical.net)
  • Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related. (elsevier.com)
  • The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography). (elsevier.com)
  • Gas chromatography is used to measure the retention times for gases injected onto a column packed with porous carbon adsorbents. (erskine.edu)
  • Rapid purification of functional cytochrome P-450 and P-450 reductase by high pressure liquid chromatography utilizing immobilized artificial membranes (IAM): A new solid phase membrane mimetic matrix. (springer.com)
  • High pressure chromatography? (physicsforums.com)
  • Related Threads on High pressure chromatography? (physicsforums.com)
  • Most of the drugs are polar in nature and preferred phase is the Reverse phase chromatography or High performance liquid chromatography. (ipl.org)
  • Moreover, high speed, selective analysis and preparative application of the sample are considered as the most significant factors driving growth of the liquid chromatography market. (openpr.com)
  • High-performance liquid chromatography is the primary method of analysis in laboratories such as forensics, toxicology, and environmental. (openpr.com)
  • It was originally developed with the use of high-performance liquid chromatography. (hindawi.com)