Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatographic techniques in which the mobile phase is a liquid.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The rate dynamics in chemical or physical systems.
An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)
A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides.
Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A series of steps taken in order to conduct research.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The chemical and physical integrity of a pharmaceutical product.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The process of cleaving a chemical compound by the addition of a molecule of water.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins prepared by recombinant DNA technology.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Compounds in which a methyl group is attached to the cyano moiety.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
The characteristic 3-dimensional shape of a carbohydrate.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
High-molecular-weight insoluble polymers that contain functional cationic groups capable of undergoing exchange reactions with anions.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Proteins found in any species of bacterium.
Established cell cultures that have the potential to propagate indefinitely.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Pyrolysis of organic compounds at the temperature of a hydrogen-air flame to produce ionic intermediates which can be collected and the resulting ion current measured by gas chromatography.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Transport proteins that carry specific substances in the blood or across cell membranes.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) purposes.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
Elements of limited time intervals, contributing to particular results or situations.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Antibodies produced by a single clone of cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
Measurement of the intensity and quality of fluorescence.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Inorganic salts of sulfuric acid.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A very strong halogenated derivative of acetic acid. It is used in acid catalyzed reactions, especially those where an ester is cleaved in peptide synthesis.
The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.
A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Compounds containing the -SH radical.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
The presence of organisms, or any foreign material that makes a drug preparation impure.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.
A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
Derivatives of GLUCURONIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the 6-carboxy glucose structure.
An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.

R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. (1/4718)

Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide assay, but retained activity as catalysts of a proline-limited protein folding reaction [Scholz, C. et al. (1997) FEBS Lett. 414, 69-73]. A reinvestigation revealed that in fact these two mutations strongly decrease the prolyl isomerase activity of Cpr3 in both the peptide and the protein-folding assay. The high folding activities found previously originated from a contamination of the recombinant Cpr3 proteins with the Escherichia coli protein SlyD, a prolyl isomerase that co-purifies with His-tagged proteins. SlyD is inactive in the peptide assay, but highly active in the protein-folding assay.  (+info)

Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme. (2/4718)

The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a potential N-terminal signal peptide that is consistent with its proposed periplasmic location. The analysis of the deduced amino acid sequence indicated that the gene sequence has a high homology (90% identity) to the Azotobacter vinelandii gene sequence, which has very recently been deposited in the GenBank database, and that it has 64% identity to the Pseudomonas aeruginosa gene sequence but that it has rather low homology (15 to 22% identity) to the gene sequences encoding alginate lyase in other bacteria. The A. chroococcum AlgL protein was overproduced in Escherichia coli and purified to electrophoretic homogeneity in a two-step chromatography procedure on hydroxyapatite and phenyl-Sepharose. The kinetic and molecular parameters of the recombinant alginate lyase are similar to those found for the native enzyme.  (+info)

EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland. (3/4718)

This study was designed to demonstrate the presence of epidermal growth factor (EGF) in the rat exorbital lacrimal gland. EGF precursor gene transcription was demonstrated first by RT-PCR analysis of lacrimal gland RNA using a set of specific primers and second by Northern blot analysis of rat lacrimal gland mRNA. A rabbit polyclonal antibody (rEGF2) directed against rat submaxillary gland EGF was used to detect EGF-containing proteins by RIA. Results indicate that the rat lacrimal gland does not contain detectable soluble and mature EGF but that the EGF immunoreactivity is associated with the membrane-enriched fraction. Analysis of the detergent-solubilized membrane proteins by gel filtration shows that membrane-associated EGF immunoreactivity was present as a high-molecular-mass protein. Moreover, as shown by Western blot analysis, a specific anti-rat EGF precursor antibody (ppEGF1) can immunoprecipitate a 152-kDa EGF-containing protein. Taken together, these results demonstrate for the first time both EGF precursor gene transcription and EGF precursor protein expression in a lacrimal tissue, i.e., the rat exorbital lacrimal gland. The demonstration that EGF appears to be stored only as its full-length membrane precursor may provide important information to study the regulation of its secretory process.  (+info)

Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes: chromatographic measurement of their membrane partition coefficients. (4/4718)

Unilamellar liposomes of small or large size, SUVs and LUVs, respectively, were stably immobilized in the highly hydrophilic Sepharose 4B or Sephacryl S-1000 gel beads as a membrane stationary phase for immobilized liposome chromatography (ILC). Lipophilic cations of triphenylmethylphosphonium and tetraphenylphosphonium (TPP+) have been used as probes of the membrane potential of cells. Interaction of TPP+ and triphenylalkylphosphonium homologues with the immobilized liposomal membranes was shown by their elution profiles on both zonal and frontal ILC. Retardation of the lipophilic cations on the liposome gel bed was increased as the hydrophobicity of the cations increased, indicating the partitioning of lipophilic cations into the hydrocarbon region of the membranes. The cations did not retard on the Sepharose or Sephacryl gel bed without liposomes, confirming that the cations only interact with the immobilized liposomes. Effects of the solute concentration, flow rate, and gel-matrix substance on the ILC were studied. The stationary phase volume of the liposomal membranes was calculated from the volume of a phospholipid molecule and the amount of the immobilized phospholipid, which allowed us to determine the membrane partition coefficient (KLM) for the lipophilic cations distributed between the aqueous mobile and membrane stationary phases. The values of KLM were generally increased with the hydrophobicity of the solutes increased, and were higher for the SUVs than for the LUVs. The ILC method described here can be applied to measure membrane partition coefficients for other lipophilic solutes (e.g., drugs).  (+info)

Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis. (5/4718)

We have constructed a library of DNA fragments heavily methylated in human adenocarcinomas of the lung to permit the comprehensive isolation of methylated CpG islands in cancer. Heavily methylated genomic DNA fragments from tumors of nine male patients were enriched using a methylated DNA binding column and used for construction of the library. From this library, DNA fragments having properties of CpG islands were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0.3% of the library were analyzed, and nine DNA fragments were identified as being associated with CpG islands that were methylated in tumor DNA. One CpG island was methylated specifically in tumor DNA, whereas the remaining eight CpG islands were methylated both in normal and tumor DNA derived from the same patients. Our results suggest that the number of CpG islands methylated specifically in tumors is not large. The library, which contains DNA fragments from methylated CpG islands comprehensively, is expected to be valuable when elucidating epigenetic processes involved in carcinogenesis.  (+info)

Deamidation of alpha-A crystallin from nuclei of cataractous and normal human lenses. (6/4718)

PURPOSE: To quantitate the extent of deamidation of asparagine-101, glutamine-50, and glutamine-6 of alpha-A crystallin in the nucleus from human cataractous and normal lenses. METHODS: Reverse phase chromatography was used to prepare alpha-A crystallin from total proteins of the nucleus from cataractous and age-matched normal human lenses. Synthetic peptides were made corresponding to the expected amidated and deamidated tryptic fragments containing asparagine-101, glutamine-50, and glutamine-6. The peptides were used to identify and quantitate amidated and deamidated forms of tryptic fragments from alpha-A crystallin eluting from a reverse phase column. RESULTS: Significant amounts of deamidation of asparagine-101 and glutamine-50, but not glutamine-6, were present in alpha-A crystallin from nuclear sections of both cataractous and age-matched normal lenses. Quantitative analysis of tryptic peptides containing these residues indicated no statistical difference in deamidation in cataractous versus normal lenses. CONCLUSIONS: There was no significant difference in the extent of deamidation of asparagine-101, glutamine-50, and glutamine-6 for alpha-A crystallin, purified from the nucleus of cataractous versus age-matched normal lenses. These results strongly suggest that deamidation of these residues does not play a role in the biogenesis of human nuclear cataract.  (+info)

Purification and properties of bovine pituitary follitropin. (7/4718)

A reproducible procedure was developed for the purification of follitropin from frozen bovine pituitary glands. The method involved precipitation with (NH4)2SO4 and acetone, followed by ion-exchange column chromatography on CM-cellulose and DEAE-cellulose and gel filtration on Sephadex G-100. A specific radioligand-receptor assay for follitropin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified bovine follitropin as measured by Steelman-Pohley bioassay was 164 times that of NIH-FSH-S1 standard preparation. They yield of bovine follitropin was 2.9 mg/kg of frozen pituitary glands. Electrophoretically, bovine follitropin was more acidic in nature and migrated further towards the anode than lutropin and thyrotropin. The elution volume of bovine follitropin by gel filtration on Sephadex G-100 was very similar to that of bovine lutropin. The amino acid composition of bovine follitropin was similar to that of sheep and human follitropin, being rich in lysine, aspartic acid, threonine, serine, glutamic acid and half-cystine.  (+info)

The selective isolation of the uterine oestradiol-receptor complex by binding to oligo(dT)-cellulose. The mediation of an essential activator in the transformation of cytosol receptor. (8/4718)

The [3H]oestradiol-receptor complex was selectively isolated from rat uterus cytosol by column chromatography on oligo(dT)-cellulose. Optimal conditions are described for the binding of the complex to oligo(dT)-cellulose, which is shown to be similar to its binding to DNA-cellulose. The cytosol complex has an apparent mol. wt. of 50,000-60,000 in high salt concentrations, as determined by Sephadex G-100 chromatography. This corresponds to the 4S cytoplasmic oestradiol receptor. In binding to oligo(dT)-cellulose the receptor is transformed into a form with an apparent mol.wt. of 100,000-120,000, corresponding to the 5S nuclear receptor complex. This transformation mimics the conversion in vivo of the cytoplasmic oestradiol receptor into the nuclear form. The binding of the complex to oligo(dT)-cellulose as a 5S nuclear form is unequivocally demonstrated to require the mediation of an activating present in the cytosol. The requirement for an activating factor is discussed in relation to reports that nuclear binding of the oestradiol-receptor complex is not dictated solely by the availability of the cytoplasmic oestradiol receptor.  (+info)

Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another. In this type of chromatography, hydrophobic groups such as phenyl, octyl, or butyl, are attached to the stationary column. Proteins that pass through the column that have hydrophobic amino acid side chains on their surfaces are able to interact with and bind to the hydrophobic groups on the column. HIC separations are often designed using the opposite conditions of those used in ion exchange chromatography. In this separation, a buffer with a high ionic strength, usually ammonium sulfate, is initially applied to the column. The salt in the buffer reduces the solvation of sample solutes thus as solvation decreases, hydrophobic regions that become exposed are adsorbed by the medium. ...
In this eBook devoted to Practical Copolymer Analysis using GPC/SEC and Related Techniques, experts discuss how several GPC/SEC methods may improve copolymer sample characterization. Learn about dual-detection methods, polymer liquid adsorption chromatography, 2D chromatography, and more ...
A convenient method is described for two-dimensional amino acid chromatography on micro scale chromatograms of 5 × 5 cm. Despite the short migration paths, excellent separation is obtained. The time of development is short and the technical procedure very simple. Extension to other classes of compounds is possible. show less ...
KROMATON designs and manufactures complete FCPC® systems dedicated to R&D laboratories, pilot plants and industrial scale productions for purification of compounds
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The Intrada Amino Acid column has been shown to be a viable solution for the analysis of amino acids by LC-MS without derivatization.
Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm) using 80 methanol as an eluent. The relevant fractions were pooled and
Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns
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Paper Chromatography - Free download as Powerpoint Presentation (.ppt), PDF File (.pdf), Text File (.txt) or view presentation slides online. Paper Chromatography Biochemistry
Column Chromatography Science Project: Investigate whether a homemade column chromatography setup can be used to separate and isolate the different food colorings that are in grape soda.
This webcast presents a series experiments in which in-process mAb material was spiked with MVM-MVP and processed through Anion Exchange and Cation Exchange, and a a viral clearance study was performed on Hydrophobic Interaction Chromatography (HIC) resins
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标签蛋白的表达是研究目标蛋白的一种重要办法。而这个可能会影响到蛋白结构和功能研究的蛋白标签往往需要被去除。该视频介绍了几种用于标签蛋白柱上酶切的试剂,以及在使用过程中的一些小技巧。. The expression of tagged proteins is the major source of proteins for research purposes. It is often recommended to remove the tag, which might otherwise interfere with protein function or interactions. This video will give you some examples of automated on-column tag removal with different cleavage agents ...
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Protein kinase C in the developing rat brain was investigated by a biochemical assay and by light-microscopic immunocytochemistry. The protein kinase was resolved on hydroxyapatite column chromatography into 3 fractions, designated types I, II, and III. Type I, with structure encoded by a gamma-sequence, was not detected early postnatally, maintained a low level of activity during the first week, which increased gradually, and reached its maximum around postnatal day 28. This type of enzyme was expressed specifically in nervous tissues, and was not found in any other tissues thus far tested. Type II enzyme activity, a mixture of the 2 subspecies encoded by the beta I- and beta II-sequences, was found at birth, increased rapidly, and reached a plateau level between postnatal days 14 and 28. This type was the predominant subspecies of protein kinase C in the brain. Type III, its structure encoded by the alpha-sequence, was also detected at birth, and reached its maximum level on postnatal day 7. ...
Introduction. Identification of amino acids by using paper chromatography Aim To separation and identification of amino acids by using paper chromatography Introduction Chromatography is a techniques separation of mixtures It involves passing the sample, a mixture which contains the analyte, in the mobile phase, often in a stream of solvent, through the stationary phase. The stationary phase retards the passage of the components of the sample. When components pass through the system at different rates they become separated in time, like runners in a mass-start foot race. Each component has a characteristic time of passage through the system, called a retention time. Chromatographic separation is achieved when the retention time of the analyte differs from that of other components mixtures in the sample. There are many types chromatography but there are four main types which are Liquid Chromatography Liquid Chromatography this is used in the world to test water samples to look for pollution ...
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HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.
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hai dear bloggers, i m here with my blog titled as COLUMN CHROMATOGRAPHY from team PHARMA WARRIORS . COLUMN CHROMATOGRAPHY column chromatography is a technique in which a column of stationary phase is used.based upon the stationary phase nature,this column chromatography is further divided into 2 types.they are if a
Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.
Dispersion due to resistance to mass transfer in the stationary phase is exactly analogous to that in the mobile phase. Solute molecules close to the interface will leave the stationary phase and enter the mobile phase before those that have diffused further into the stationary phase and have a longer distance to diffuse back. Thus, as those molecules that were close to the surface will be swept along in the moving phase, they will be dispersed from those molecules still diffusing to the surface. The dispersion resulting from the resistance to mass transfer in the stationary phase is depicted in figure 23. Molecules 1 and 2 (the two closest to the surface) will enter the mobile phase and begin moving along the column. Their movement will continue while molecules 3 and 4 diffuse to the interface at which time they will enter the mobile phase and start following molecules 1 and 2 down the column. ...
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Prepacked Chromatography Columns Market was US$1.74 bn in 2016 and is poised to reach US$3.34 bn by 2024, expand at a CAGR of 8.4% therein
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Definition of Paper chromatography with photos and pictures, translations, sample usage, and additional links for more information.
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The core houses the inner frit, through which the eluent percolates and exits at the base of the column to a detector and hence to a fraction collector. The outer frit constitutes the column inlet, and consequently the sample has initially an extremely large area of stationary phase with which to interact. This renders the loading capacity of the radial flow column also very high. It is interesting to note, that as the solute progress radially through the stationary phase bed towards the center, the effective cross-sectional area of the column will become smaller. Consequently, the plate volume of the column will decrease (see Plate Theory and Extensions ) as the solute moves to the center which will result in the solute being concentrated. However, as the solute bands progressively decrease in concentration due to normal dispersion processes (see Dispersion in Chromatography Columns ), this counteracts the concentration effect from reduced bed cross-section and prevents the column packing from ...
Chromatography are Cost-effective and innovative chromatographic purification platforms. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step. Explore our solutions.
A major Liquid Chromatography mode in which samples are separated by virtue of their size in solution. Also known as size-exclusion, gel permeation, gel fi
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Hi I have attached a sheet as an example, as i am trying to Divide a cell by the number of populated cells in that column e.g column (A) has a table of 16 but only has 13 populated cells in that column, the sum would be something like this =sum(A18/13. Sometimes the column will have less and at times more populated. Hopefully its clear to you Thanks
Hi, I have sequential numbers below in column A of excel. M66.211 M66.212 M66.213 M66.214 M66.215 M66.216 M66.217 M66.218 M66.219 M66.811 M66.812 M66.
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Dengue IgG/IgM Rapid Test Kit is a rapid immunochromatographic assay for the simultaneous detection of IgG and IgM antibodies to dengue virus in human whole blood, serum or plasma. The assay is used as a screening test for Dengue viral infection and as an aid for differential diagnosis of primary and secondary infections in conjunction with other criteria.
An improved method for qualitative analysis of mixtures containing chloride, chlorite, chlorate, and perchlorate has been developed. This method utilizes paper partition chromatography and differential spray reagents for location of the separated spots. (Author)(*CHLORATES
A method for determining the concentration of analyte in a test fluid by immunochromatography techniques which involves quantitatively determining the signals from captured analyte/labeled binding partner complex by an instrument, e.g. reflectance spectrometer. In a preferred embodiment, a reflectance reading is determined for the captured complex and uncomplexed labeled binding partner which is captured in a separate zone of the immunochromatographic strip and the ratio of these reflectances is used to provide additional quantitation to the assay method.
A method for determining the concentration of analyte in a test fluid by immunochromatography techniques which involves quantitatively determining the signals from captured analyte/labeled binding partner complex by an instrument, e.g. reflectance spectrometer. In a preferred embodiment, a reflectance reading is determined for the captured complex and uncomplexed labeled binding partner which is captured in a separate zone of the immunochromatographic strip and the ratio of these reflectances is used to provide additional quantitation to the assay method.
Part of the Technology Updates series, this fact sheet describes PATHs work to develop a rapid, easy-to-read, low-cost test for Chagas disease.. Corporate author(s): PATH. Publication date: July 2013. ...
Chromatographic immunoassay employing a specific binding pair member and a label conjugate which delineates a border whose distance from one end of the chromatograph relates to the amount of analyte present. By combining the label conjugate and sample in a solution and immunochromatographing the solution, or employing a combination of enzymes, one enzyme being the label and the other enzyme affixed to the chromatographic support, the position of the border defined by the label can be related to the amount of analyte in the sample solution. Preferably, an immunochromatograph is employed having both a specific binding pair member and an enzyme affixed to the support. A sample is chromatographed and the amount of analyte is determined by (1) contacting the chromatograph with a second enzyme conjugated with a specific binding pair member which binds to the chromatograph in proportion to the amount of analyte bound to the chromatograph, or (2) including the second enzyme conjugate with said sample, resulting
Novel compounds are produced by the fermentation of a nutrient medium with the previously undescribed microorganism Streptomyces avermitilis. They may be isolated by solvent extraction and chromatographic fractionation techniques. The compounds, which are described generically as C-076 have significant parasiticidal activity. The compounds may be included in compositions for the oral or parenteral administration to animals for the prevention and cure of parasitic infections.
POLYSTYRENE:62,,GRADIENT INTERACTION CHROMATOGRAPHY:56,,ANIONICPOLYMERIZATION:54,,SEPARATION:46,,LIQUIDCHROMATOGRAPHY:38,,SIZEEXCLUSION CHROMATOGRAPHY:34,,BEHAVIOR:34,,POLYMERS:32,,BLOCKCOPOLYMERS:28,,POLYISOPRENE:24,,DIBLOCK COPOLYMERS:24,,HPLC:22,,MOLECULARWEIGHT DISTRIBUTION:20,,THINFILMS:18,,PERFORMANCE LIQUIDCHROMATOGRAPHY:18,,SYNTHETICPOLYMERS:18,,MACROMOLECULES:18,,REVERSEDPHASE:16,,RETENTION BEHAVIOR:16,,MOLECULARWEIGHT:14,,block copolymer:14,,MORPHOLOGY:12,,CRITICAL ADSORPTION POINT:12,,BLOCKCOPOLYMER:12,,MELTS:12,,COPOLYMERS:12,,DIBLOCK COPOLYMER:12,,LIGHTSCATTERING:10,,DEPENDENCE:10,,ADSORPTION CHROMATOGRAPHY:10,,COMPLEX POLYMERS:10,,polystyrene:10,,temperature gradient interaction chromatography:10,,ETHYLENEOXIDE:10,,GELPERMEATION CHROMATOGRAPHY:10,,ELUTION:10,,FRACTIONATION:10,,AIR/WATER INTERFACE:10,,XRAYSCATTERING:10,,POLYMERIZATION:10,,POLYMER CHARACTERIZATION:10,,MONOLAYERS:10,,AIRWATERINTERFACE:8,,PROBE DIFFUSION:8,,DYNAMICS:8,,MIXTURES:8,,AROMATIC ...
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Gravity chromatography column manufactured from medium wall tubing with PTFE stopcock and in-line reservoir for containing liquid buffer.
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a vertical glass tube used in column chromatography; a mixture is poured in the top and washed through a stationary substance where components of the mixture are adsorbed selectively to form colored bands. ...
Raptor ARC-18 LC Columns (USP L1) cat.# 9314232 Ideal for high-throughput LC-MS/MS applications with minimal sample preparation.Well-balanced retention profile ...
Simple Paper Chromatography Experiment. Best custom written essays, term papers and a lot more! Buy Cheap high-quality research papers, thesis papers, book reports.
Its interaction with DNA was studied using a combination of S(1) nuclease hydrolysis and hydroxyapatite chromatography. Prognostic value of elevated white blood cell count sildenafil sandoz 100 mg in hypertension. The findings provide the first direct evidence suggesting that the functional ...
View Notes - Exp 2 Lab Report from CHM 102 at Rhode Island. Rachel Golub CHM 102 Experiment 2: Paper Chromatography 09-27-06 Lab Report INTRODUCTION: In this experiment, I will be using a variety
Paper chromatography is one of the easiest methods of chromatography. It is a method of planar chromatography (stationary phase is in form of a plane).
PLATE 32 FIG. 3. Portion of astronomical ceiling (Sen-n-mut). (his, xiv (1930), PI. 1^9.) Jupiter, Sirius, and Orion are shown in three successive columns starting from the left. The three conspicuous stars almost in a vertical line seem to be S, e, and Orionis. The Egg near the centre may represent the Pleiades and the V-shaped group between Orion and the Egg the Hyades. Above is part of the dekan list ...
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BIA Separations is the leading developer and manufacturer of CIM® (Convective Interaction Media) monolithic chromatographic columns for production, purification, and analytics of large biomolecules.
BIA Separations is the leading developer and manufacturer of CIM® (Convective Interaction Media) monolithic chromatographic columns for production, purification, and analytics of large biomolecules.
labfilter - Ahlstrom Grade 631 is a think chromatography paper with a faster flow rate It is recommended for routine chromatography where loading is relatively low Thickness: 023 mm Absorption Speed: 150 mm / 30 min Ahlstrom manufactures a complete line of hi
Contact chromatography today the equipment publication which contains news and articles on the latest techniques and products for the chromatography market
[121 Pages Report] Check for Discount on United States Chromatography Instruments Market Report 2016 report by QYResearch Group. Notes: Sales, means the sales volume of Chromatography Instruments Revenue,...
Located in California, SC Chromatography provides various chromatography services including moving, installation, repairs, maintenance, training, method development and equipment qualification. They proudly service Agilent instruments.
What elutant to use? These kind of columns are typically run isocraticly (one solvent for entire separation) or by step-elution (abrupt solvent change).
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Please note that in this PDF only the phase description and the corresponding article numbers for the different phases are displayed. For a complete column in your desired dimension, please take a look at the Order Information for our columns ...
Please note that in this PDF only the phase description and the corresponding article numbers for the different phases are displayed. For a complete column in your desired dimension, please take a look at the Order Information for our columns. ...
This seems like it should be easy enough, but I cant seem to find an easy answer. I have information on column A and column B but I want the cells in column B to be highlighted if it doesnt match column A. I could do it one at a time but with a few hundred lines, it would take a while ...
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Click on column heading text to sort by a column. Initial column will be sorted in descending order, click again to get ascending order. The type of data in the cell is determined automatically: ...
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Im working with a document I was given. There are currently 4 columns with cells of a certain size. When I try to insert a new column, it comes in
Almsherqi, Z.A., Mclachlan, C.S., Sharef, S.M. (2006-07). More on: Enhanced antiplatelet effect of clopidogrel in patients whose platelets are least inhibited by aspirin: A randomized crossover trial [12]. Journal of Thrombosis and Haemostasis 4 (7) : 1638-1639. [email protected] Repository. https://doi.org/10.1111/j.1538-7836.2006.01926. ...
George Field - Chromatography; or, a Treatise on Colours and Pigments, and of their Powers in Painting. Théodore Chassériau ... 127-8. ISBN 0-7181-1279-2. George Field (1841). Chromatography; Or, A Treatise on Colours and Pigments: And of Their Powers in ...
"Chemical Heritage Foundation Presents Ahmed Zewail with Othmer Gold Medal". Chromatography Techniques. 27 January 2009. ...
Countercurrent Chromatography is a method of separation, that is based on the differential partitioning of analytes between two ... The term partition chromatography is largely a synonymous and predominantly used for hydrostatic CCC instruments. Distillation ... "Countercurrent Chromatography". University of Illinois at Chicago. Retrieved 16 April 2011. Benzer Seymour (1967). "Behavioral ... the most widely used term and abbreviation is CounterCurrent Chromatography or CCC, in particular when using hydrodynamic CCC ...
Biomedical Chromatography. 23 (12): 1266-75. doi:10.1002/bmc.1249. PMID 19488979. Zimmermann-Tansella C, Tansella M, Lader M ( ... "Quantification of chlordesmethyldiazepam by liquid chromatography-tandem mass spectrometry: application to a cloxazolam ...
Biomedical Chromatography. 24 (8): 887-92. doi:10.1002/bmc.1382. PMID 20033890.. ...
Ettre, C. (2001). "Milestones in Chromatography: The Birth of Partition Chromatography" (PDF). LCGC. 19 (5): 506-512. Archived ... and in techniques that laid the foundation for several new types of chromatography. He developed partition chromatography ... Martin, A J P; Synge, R L M (1941). "A new form of chromatogram employing two liquid phases A theory of chromatography. 2. ... "Gas Chromatography-Mass Spectrometry". American Chemical Society. Retrieved 19 November 2019. "Nobel Winner Archer Martin Dies ...
Journal of Chromatography. 145 (3): 464-8. doi:10.1016/s0378-4347(00)81377-8. PMID 659533. Tangerman, A; Meuwese-Arends, MT; ...
Journal of Chromatography. 581 (2): 171-178. doi:10.1016/0378-4347(92)80269-V. PMID 1452607. Pfeiffer S.; Milne S.; Stevenson R ...
Primary and secondary bonds: optimal conditions for association and dissociation". Journal of Chromatography. 376: 111-9. PMID ...
Journal of Chromatography. 305 (1): 135-143. doi:10.1016/S0378-4347(00)83321-6. PMID 6707137. Methling, K; Reszka P; Lalk M; ... "Quantitation of flupirtine and its active acetylated metabolite by reversed-phase high-performance liquid chromatography using ...
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 907: 168-72. doi:10.1016/j.jchromb. ...
These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size- ... exclusion chromatography. Desalting and buffer exchange are two of the most common gel filtration chromatography applications, ... electrophoresis or affinity chromatography. Size exclusion chromatography applications for separating macromolecules based on ... Chromatography columns Gravity-flow columns Chromatography cartridges Centrifuge columns Centrifuge plates Gravity-flow, or ...
... oxime-trimethylsilyl ester derivatization and cation-exchange chromatography". Journal of Chromatography B. 719 (1-2): 1-7. doi ... Journal of Chromatography B. 758 (1): 87-94. doi:10.1016/s0378-4347(01)00101-3. PMID 11482739. Lee, SH; Kim, SO; Chung, BC ( ... Journal of Chromatography. 430 (2): 223-31. doi:10.1016/s0378-4347(00)83157-6. PMID 3235498. European Nutrigenomics ...
... studied in rat urine using gas chromatography-mass spectrometry". Journal of Chromatography. B, Analytical Technologies in the ...
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 879 (31): 3771-4. doi:10.1016/j. ...
Using the technique of paper chromatography, Hofmann confirmed the presence of 0.25% (by weight) psilocybin in dried samples. ... Journal of Chromatography. 312: 467-72. doi:10.1016/s0021-9673(01)92800-6. PMID 6543215. Jokiranta J, Mustola S, Ohenoja E, ... determination of hallucinogenic components of Psilocybe mushrooms by reverse-phase high-performance liquid chromatography". ...
"Doping control for metandienone using hair analyzed by gas chromatography-tandem mass spectrometry". Journal of Chromatography ... injection of depot nandrolone decanoate using dried blood spots sampling coupled with ultrapressure liquid chromatography ...
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 812 (1-2): 291-307. doi:10.1016/j. ...
Thin-layer chromatography and electrophoresis screening of mushrooms with electron spin resonance determination of the toxin". ... Journal of Chromatography. 758 (1): 145-157. doi:10.1016/S0021-9673(96)00695-4. PMID 9181972.CS1 maint: uses authors parameter ...
... studied in rat urine using gas chromatography-mass spectrometry". Journal of Chromatography. B, Analytical Technologies in the ... Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 793 (2): 331-42. doi:10.1016/S1570- ... methoxy-alpha-pyrrolidinopropiophenone studied in rat urine using gas chromatography-mass spectrometry". ...
Thin-layer chromatography and electrophoresis screening of mushrooms with electron spin resonance determination of the toxin". ... Journal of Chromatography. 758 (1): 145-57. doi:10.1016/S0021-9673(96)00695-4. PMID 9181972. Peintner U, Horak E, Moser M, ...
The analyte in solution is introduced from a direct inlet probe or a liquid chromatography (LC) eluate into a pneumatic ... The sensitivity of APCI combined with the sensitivity and specificity of LC/MS and liquid chromatography-tandem mass ... Niessen, Wilfried (2006). Liquid Chromatography Mass spectrometry. 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL ... Holčapek, Michal; Jirásko, Robert; Lísa, Miroslav (2012). "Recent developments in liquid chromatography-mass spectrometry and ...
John Knox was an early leader in the field of gas chromatography. As a PhD student in at Pembroke College, Cambridge, in 1953 ... During a sabbatical with Prof JC Giddings in Utah in 1964, Knox was introduced to column liquid chromatography. Back home in ... In later experiments Knox was the first to use gas chromatography to measure rate of reaction constants for gaseous chemical ... In the 1970s Knox and his Edinburgh research group invented new micro-particulate packing materials for liquid chromatography, ...
This led to the development of Gas chromatography-mass spectrometry instruments such as the Bendix MA-2 Time-of-Flight Mass ... "Gas Chromatography-Mass Spectrometry". American Chemical Society. Retrieved 19 Nov 2019. CS1 maint: discouraged parameter (link ... Harrington at Bendix Aviation to demonstrate the potential of combining gas chromatography and mass spectrometer. ...
For example, gas chromatography-mass spectrometry, gas chromatography-infrared spectroscopy, liquid chromatography-mass ... Holt, R. M.; Newman, M. J.; Pullen, F. S.; Richards, D. S.; Swanson, A. G. (1997). "High-performance Liquid Chromatography/NMR ... Chromatography, electrophoresis and field flow fractionation are representative of this field. Combinations of the above ... Most often the other technique is some form of chromatography. Hyphenated techniques are widely used in chemistry and ...
ISBN 978-1-60774-172-5. Linskens, Hans-Ferdinand; Jackson, John F. (2012-12-06). Gas Chromatography/Mass Spectrometry. Springer ...
E. Heftmann (26 November 1991). Journal of Chromatography Library. Elsevier. pp. 2-. ISBN 978-0-08-085859-3. Retrieved 6 May ...
Analysis of the samples typically involves three parts; preparation, chromatography and detection. Preparation involves removal ...
"The nitrogen-phosphorus detector and its applications in gas chromatography". Journal of Chromatography A. 134 (1): 57-64. doi: ... Since the alkali metal bead is consumed over time, it must be replaced regularly . Gas chromatography Wolfgang Kleiböhmer (2001 ... is a detector commonly used with gas chromatography, in which thermal energy is used to ionize an analyte. It is a type of ...
Loading sample on chromatography columns. Certain types of two-stroke and four-stroke engines. Most hydraulic automotive power ... Rotary valves are used for loading samples on columns used for liquid or gas chromatography. The valves used in these methods ...
... paper chromatography, and gas-liquid chromatography which is more commonly known as gas chromatography. The modification of ... The introduction of paper chromatography was an important analytical technique which gave rise to thin-layer chromatography. ... Pakhomov, V. P. (2003). "Chromatography in Pharmaceutical Chemistry (100 Years of the Discovery of Chromatography by M. S. ... A theory of chromatography. 2. Application to the micro-determination of the higher monoamino-acids in proteins". Biochemical ...
Gas chromatography is also sometimes known as vapor-phase chromatography (VPC), or gas-liquid partition chromatography (GLPC). ... chromatography, is therefore credited for the foundation of gas chromatography. The popularity of gas chromatography quickly ... Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds ... Gas chromatography is the process of separating compounds in a mixture by injecting a gaseous or liquid sample into a mobile ...
Thin layer chromatography (TLC)[edit]. Further information: Thin layer chromatography. Thin layer chromatography (TLC) is a ... Gas chromatography[edit]. Further information: Gas chromatography. Gas chromatography (GC), also sometimes known as gas-liquid ... Affinity chromatography[edit]. Further information: Affinity chromatography. Affinity chromatography[14] is based on selective ... Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and ...
Size exclusion chromatography. Micellar liquid chromatography. Ion chromatography (or ion-exchange chromatography) separates ... Anion exchange chromatography retains anions using positively charged functional group: R-X. +. A. −. +. M. +. B. −. ⇄. R-X. + ... or in chromatography columns. Thin layer chromatography or column chromatography share similarities in that they both act ... This type of chromatography is further subdivided into cation exchange chromatography and anion-exchange chromatography. ...
The principle of immunoaffinity or immunoadsorption chromatography is based on the highly specific interaction of an antigen ... with its antibody (1). Immunoaffinity chromatography is a specialized form... ... Immunoaffinity chromatography is a specialized form of affinity chromatography and, as such, utilizes an antibody or antibody ... Phillips, T. M. (1989). High-performance immunoaffinity chromatography. Adv. Chromatogr. 29, 133-173.PubMedGoogle Scholar ...
Chromatography is a way of separating two or more chemical compounds. Chemical compounds are chemicals that are bonded together ... These include gas, high pressure liquid, or ion exchange chromatography.. In general, chromatography uses the differences in ... Chromatography is a way of separating two or more chemical compounds. Chemical compounds are chemicals that are bonded together ...
... chromatography: Subsequent developments: A technique exhibiting great selectivity, affinity chromatography, was first described ... In chromatography: Retention mechanism. Affinity chromatography exploits this feature by binding a ligand with the desired ... In chromatography: Subsequent developments. A technique exhibiting great selectivity, affinity chromatography, was first ... interactive capability to a support such as a gel used in gel-filtration chromatography. The ligand retards a solute with the ...
... is used for water chemistry analysis. Ion chromatographs are able to measure concentrations of major anions ... Created by Monica Z. Bruckner, Montana State University What is Ion Chromatography? ... Ion Chromatography. Created by Monica Z. Bruckner, Montana State University. What is Ion Chromatography?. Ion chromatography is ... How Does Ion Chromatography Work?. Ion chromatography, a form of liquid chromatography, measures concentrations of ionic ...
As a chromatography consumable manufacturer and a contract manufacturing organization (CMO), Regis has an onsite team of ... If chiral separation by supercritical fluid chromatography is best, Regis will handle the project in its state of the art SFC ... This valuable addition to its core expertise in chiral chromatography provides a fast and effective advantage that can tackle ... RegisSEP™ Chiral Separation Service uses supercritical fluid chromatography, a method that uses carbon dioxide for a faster and ...
The present communication deals with the application of paper partition chromatography to the identification of natural ... APART from an early attempt by other authors1-3 to adapt a method of paper chromatography for the examination of natural ... APART from an early attempt by other authors1-3 to adapt a method of paper chromatography for the examination of natural ... The present communication deals with the application of paper partition chromatography to the identification of natural ...
Immobilized artificial membrane chromatography: supports composed of membrane lipids. Anal. Biochem. 176: 36-47.PubMedCrossRef ... Chae WG., Luo C., Rhee D.M., Lombardo C.R., Low P., Pidgeon C. (1991) Immobilized Artificial Membrane Chromatography. In: ... Immobilized Artificial Membrane Chromatography. Initial Studies Using Monomyristoylphosphatidylcholine as a Detergent for ... Use of high-performance size exclusion chromatography to determine the extent of detergent solubilization of human erythrocyte ...
... chromatography: Development chromatography: In terms of operation, in development chromatography the mobile phase flow is ... In chromatography: Development chromatography. In terms of operation, in development chromatography the mobile phase flow is ... In elution chromatography successive samples of the effluent are collected in tubes held in a mechanically driven rotating tray ... In chromatography: Sample recovery. Sample recovery from development chromatograms has been described-that is, detection ...
... the different pigments present in a leaf are separated using paper chromatography. ... This experiment works very well providing care is taken over preparing the spot on the chromatography paper. It should be as ... d On a strip of chromatography paper, draw a pencil line 3 cm from the bottom.. ... i Avoid moving the beaker in any way once the chromatography has started.. ...
Find current solutions to practical chromatographic separations and learn how to optimize chromatography processes for the best ... conference focusing on the most cutting-edge areas of preparative and process chromatography. ... Preparative and Process Chromatography. Most recognized international conference in the world for the presentation and ... Keynote Session on Industrial Case Studies in Protein Chromatography - Co-chairs: A. Hunter, T. Pabst (MedImmune) ...
These include hydrophobic interaction chromatography, reversed phase chromatography, two-dimensional chromatography and many ... Ion exchange chromatography. One of the most popular methods for separating proteins is ion exchange chromatography. It ... Affinity chromatography. Affinity chromatography is based on binding interactions between a protein and a ligand immobilized to ... Size exclusion chromatography. Related Stories. *High-performance porous polymeric material for chromatography applications ...
There are a variety of uses for affinity chromatography purification techniques. They are being increasingly used to separate ... Principles of Chromatography. Play. Lectin Affinity Chromatography. This form of chromatography is growing in use. Lectins are ... Immobilized Metal Ion Affinity Chromatography. Immobilized metal ion affinity chromatography (IMAC) involves binding with ... www.sigmaaldrich.com/life-science/proteomics/protein-chromatography/affinity-chromatography.html#applications ...
Title: IONIC CHROMATOGRAPHY STANDARDS, Author: Stephane Schein, Name: IONIC CHROMATOGRAPHY STANDARDS, Length: 29 pages, Page: 1 ... IONIC CHROMATOGRAPHY STANDARDS. BARIUM MONO-ELEMENT STANDARD FOR IONIC CHROMATOGRAPHY Element: Baryum - Ba2+ Ref.: A 005 100 ... IONIC CHROMATOGRAPHY STANDARDS CONCENTRATED ELUENTS FOR IONIC CHROMATOGRAPHY Solution: 0,18M Carb./ 0,17M Bicarb. Ref.: A 105 ... IONIC CHROMATOGRAPHY STANDARDS MONO-ELEMENT STANDARD FOR IONIC CHROMATOGRAPHY Element: Succinate - OOC(CH2)2COO2Ref.: A 044 100 ...
These look the same on paper but have different chromatography results. ... Outreach: crime scene chromatography Description. The best pens for this activity are (1) A black felt tip pen (2) A black ... Outreach: chromatography, how black is a black pen? Experiment with the Vikings FunKids Radio: Kareenas Chemistry Outreach: ... These look the same on paper but have different chromatography results. The best pens for this activity are (1) A black felt ...
Readers will discover how DNA chromatography can be used in their own work. Intended as an introduction for ... ... DNA chromatography. [Douglas T Gjerde; Christopher P Hanna; D Hornby; John Wiley & Sons.] -- This book describes a powerful and ... Chromatography, High Pressure Liquid. a schema:Intangible ;. schema:name "Chromatography, High Pressure Liquid"@en ;. .. ... DNA chromatography. Author:. Douglas T Gjerde; Christopher P Hanna; D Hornby; John Wiley & Sons.. ...
... column chromatography) or a strip of filter paper (paper chromatography) or by a gel. See more. ... Chromatography definition, the separation of mixtures into their constituents by preferential adsorption by a solid, as a ... chromatography. Historical Examples. of chromatography. *. Mr Field, in his Chromatography, has rendered a very great service ... Origin of chromatography. First recorded in 1725-35; chromato- + -graphy. Related formschro·ma·tog·ra·pher, nounchro·mat·o· ...
GE Healthcare Blue Sepharose™ 6 Fast Flow Chromatography Media Blue dye binds to proteins such as albumin and lipoproteins as ... GE Healthcare Capto™ Multimodal (MMC) Chromatography Media Used to solve specific purification challenges at both high and low ... GE Healthcare MabSelect Sure™ LX Chromatography Media Protein A affinity medium with high dynamic binding capacity which ... GE Healthcare Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media USe for separations of biomolecules with an affinity ...
... to form a fluid tight seal between the polymer cap and container suitable for use in low pressure liquid chromatography. ... Column for liquid chromatography. US4758340. 19 Oct 1987. 19 Jul 1988. Labomatic Ag. Sealing device for a chromatography column ... Chromatography column. US5601708. 14 Sep 1995. 11 Feb 1997. Dyax Corp.. Apparatus for pressurizing a removable chromatography ... Multipart chromatography column connection. US5238556. 13 May 1992. 24 Aug 1993. Hamid Shirkhan. Chromatography tube for use ...
ANION CHROMATOGRAPHY. Scope and Conditions for Separation. Suppressed Anion Chromatography. Nonsuppressed Ion Chromatography. ... Applications of DNA Chromatography. Applications of RNA Chromatography. SAMPLE PRETREATMENT. Dilute and Shoot or Pre-Treat the ... DNA AND RNA CHROMATOGRAPHY. Introduction. DNA and RNA Chemical Structure and Properties. DNA and RNA Chromatography. ... Chelating Ion-Exchange Resins and Chelation Ion Chromatography. ION-EXCLUSION CHROMATOGRAPHY. Principles. Separation of Organic ...
Purchase Affinity Chromatography and Biological Recognition - 1st Edition. Print Book & E-Book. ISBN 9780121665807, ... Affinity Chromatography of Interferons-A Novel Application for Calmodulin-Sepharose. Dextran-Sepharose Affinity Chromatography ... Subunit Exchange Chromatography: Principles and Applications. Affinity Chromatography Studies of Cooperative Interactions in ... IMA-Chromatography (Immobilized Ion Affinity Chromatography): Reflections of Methodological Development. Preparative and ...
Does the red in Skittles look the same as the red in M&Ms when you test both using paper chromatography? What about their other ... Candy Chromatography: What Makes Those Colors?, from Science Buddies. This activity brought to you in partnership with Science ... In each chromatography setup there is generally a so-called mobile element (a fluid in which the components are dissolved) and ... In paper chromatography different pigments can be separated based on their solubility, or their ability to dissolve in water. ...
To reduce chromatography complexity and conserve costly Protein A, CMC Biologics evaluated multicolumn solutions and selected ... Multicolumn Chromatography. While the concept of multicolumn chromatography-also referred to as simulated moving bed (SMB) ... Protein A capture chromatography is a crucial step in all downstream processing of mAbs. The process provides a highly ... In application, multicolumn chromatography allows for product breakthrough in the load zone of the first column, with the ...
PRNewswire/ -- According to a new market research report Gas Chromatography Market by Instrument (Systems, Detectors), ... Browse 86 market data Tables and 31 Figures spread through 167 Pages and in-depth TOC on Gas Chromatography Market ... According to a new market research report Gas Chromatography Market by Instrument (Systems, Detectors), Accessories and ... Chromatography Instruments Market by System (LC (HPLC, UHPLC, Flash), GC, Other Components (Auto samplers, Detectors, Fraction ...
... Liquid chromatography (LC) testing and analysis.. Intertek provides Liquid Chromatography (LC) ... Liquid chromatography allows for selective and highly sensitive detection of trace and molecular-species specific compounds. ...
... Anthea Scothern anthea.scothern at umist.ac.uk Wed Dec 13 04:37:21 EST 2000 *Previous message: ...
Ultra-fast gas chromatography means analysis cycle times are decreasing so the time to prepare the samples to be analysed is ... Agilent expands chromatography capabilities in California 16-Dec-2016. By Joseph James Whitworth ... Phenomenex opens gas chromatography facility 01-Jun-2017. By Joseph James Whitworth ... SCION Instruments has set its sights on tailoring the capabilities of gas chromatography systems to meet specific analytical ...