A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA. It is comprised of three different subunits of 48, 60, and 150 kDa molecular size. The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.
Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A retinoblastoma-binding protein that has an affinity for core HISTONES. It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The process by which a DNA molecule is duplicated.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins involved in the assembly and disassembly of HISTONES into NUCLEOSOMES.
Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins found in any species of fungus.
Formation of an acetyl derivative. (Stedman, 25th ed)
A subunit of the anaphase-promoting complex whose primary function is to provide structural support for the catalytic and substrate-recognition modules of the complex. Apc5, along with Apc4, tethers the tetratricopeptide-coactivator binding subcomplex to the main structural subunit, Apc1.
A family of histone molecular chaperones that play roles in sperm CHROMATIN decondensation and CHROMATIN ASSEMBLY in fertilized eggs. They were originally discovered in XENOPUS egg extracts as histone-binding factors that mediate nucleosome formation in vitro.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Circular duplex DNA isolated from viruses, bacteria and mitochondria in supercoiled or supertwisted form. This superhelical DNA is endowed with free energy. During transcription, the magnitude of RNA initiation is proportional to the DNA superhelicity.
An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.
The large subunit of the 80s ribosome of eukaryotes. It is composed of the 28S RIBOSOMAL RNA, the 5.8S RIBOSOMAL RNA, the 5S RIBOSOMAL RNA, and about 50 different RIBOSOMAL PROTEINS.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
Preparations of cell constituents or subcellular materials, isolates, or substances.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Established cell cultures that have the potential to propagate indefinitely.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A flavoprotein and iron sulfur-containing oxidoreductase complex that catalyzes the conversion of UBIQUINONE to ubiquinol. In MITOCHONDRIA the complex also couples its reaction to the transport of PROTONS across the internal mitochondrial membrane. The NADH DEHYDROGENASE component of the complex can be isolated and is listed as EC 1.6.99.3.
An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.
An essential amino acid. It is often added to animal feed.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.
Diseases caused by abnormal function of the MITOCHONDRIA. They may be caused by mutations, acquired or inherited, in mitochondrial DNA or in nuclear genes that code for mitochondrial components. They may also be the result of acquired mitochondria dysfunction due to adverse effects of drugs, infections, or other environmental causes.
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
Proteins prepared by recombinant DNA technology.
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
An order of anaerobic methanogens in the kingdom EURYARCHAEOTA. They are pseudosarcina, coccoid or sheathed rod-shaped and catabolize methyl groups. The cell wall is composed of protein. The order includes one family, METHANOCOCCACEAE. (From Bergey's Manual of Systemic Bacteriology, 1989)

Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. (1/113)

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.  (+info)

WD repeats of the p48 subunit of chicken chromatin assembly factor-1 required for in vitro interaction with chicken histone deacetylase-2. (2/113)

Chromatin assembly factor-1 (CAF-1) is essential for chromatin assembly in eukaryotes, and comprises three subunits of 150 kDa (p150), 60 kDa (p60), and 48 kDa (p48). We cloned and sequenced cDNA encoding the small subunit of the chicken CAF-1, chCAF-1p48. It consists of 425 amino acid residues including a putative initiation Met, possesses seven WD repeat motifs, and contains only one amino acid change relative to the human and mouse CAF-1p48s. The immunoprecipitation experiment followed by Western blotting revealed that chCAF-1p48 interacts with chicken histone deacetylases (chHDAC-1 and -2) in vivo. The glutathione S-transferase pulldown affinity assay revealed the in vitro interaction of chCAF-1p48 with chHDAC-1, -2, and -3. We showed that the p48 subunit tightly binds to two regions of chHDAC-2, located between amino acid residues 82-180 and 245-314, respectively. We also established that two N-terminal, two C-terminal, or one N-terminal and one C-terminal WD repeat motif of chCAF-1p48 are required for this interaction, using deletion mutants of the respective regions. These results suggest that chCAF-1p48 is involved in many aspects of DNA-utilizing processes, through alterations in the chromatin structure based on both the acetylation and deacetylation of core histones.  (+info)

Chromatin replication: Finding the right connection. (3/113)

Nucleosomes are preferentially assembled on replicating DNA by chromatin assembly factor 1; recent studies have shown that replicated DNA is marked for assembly into chromatin by the replication-fork-associated protein PCNA.  (+info)

Chromatin assembly during DNA replication in somatic cells. (4/113)

Newly replicated DNA is assembled into chromatin through two principle pathways. Firstly, parental nucleosomes segregate to replicated DNA, and are transferred directly to one of the two daughter strands during replication fork passage. Secondly, chromatin assembly factors mediate de-novo assembly of nucleosomes on replicating DNA using newly synthesized and acetylated histone proteins. In somatic cells, chromatin assembly factor 1 (CAF-1) appears to be a key player in assembling new nucleosomes during DNA replication. It provides a molecular connection between newly synthesized histones and components of the DNA replication machinery during the S phase of the cell division cycle.  (+info)

Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins. (5/113)

Mechanisms contributing to the maintenance of heterochromatin in proliferating cells are poorly understood. We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome assembly during DNA replication. Mutations in p150 prevent association with HP1 in heterochromatin in cells that are not in S phase and the formation of CAF-1-HP1 complexes in nascent chromatin during DNA replication in vitro. We suggest that CAF-1 p150 has a heterochromatin-specific function distinct from its nucleosome assembly function during S phase. Just before mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin concomitant with histone H3 phosphorylation. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.  (+info)

Duplication and maintenance of heterochromatin domains. (6/113)

To investigate the mechanisms that assure the maintenance of heterochromatin regions, we took advantage of the fact that clusters of heterochromatin DNA replicate late in S phase and are processed in discrete foci with a characteristic nuclear distribution. At the light microscopy level, within these entities, we followed DNA synthesis, histone H4 acetylation, heterochromatin protein 1 (Hp1alpha and -beta), and chromatin assembly factor 1 (CAF-1). During replication, Hp1alpha and -beta domains of concentration are stably maintained, whereas heterochromatin regions are enriched in both CAF-1 and replication-specific acetylated isoforms of histone H4 (H4Ac 5 and 12). We defined a time window of 20 min for the maintenance of this state. Furthermore, treatment with Trichostatin A (TSA), during and after replication, sustains the H4Ac 5 and 12 state in heterochromatin excluding H4Ac 8 and 16. In comparison, early replication foci, at the same level, did not display any specific enrichment in H4Ac 5 and 12. These data emphasize the specific importance for heterochromatin of the replication-associated H4 isoforms. We propose that perpetuation of heterochromatin involves self-maintenance factors, including local concentration of Hp1alpha and -beta, and that a degree of plasticity is provided by the cycle of H4 acetylation/deacetylation assisted by CAF-1.  (+info)

A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage. (7/113)

Sensing DNA damage is crucial for the maintenance of genomic integrity and cell cycle progression. The participation of chromatin in these events is becoming of increasing interest. We show that the presence of single-strand breaks and gaps, formed either directly or during DNA damage processing, can trigger the propagation of nucleosomal arrays. This nucleosome assembly pathway involves the histone chaperone chromatin assembly factor 1 (CAF-1). The largest subunit (p150) of this factor interacts directly with proliferating cell nuclear antigen (PCNA), and critical regions for this interaction on both proteins have been mapped. To isolate proteins specifically recruited during DNA repair, damaged DNA linked to magnetic beads was used. The binding of both PCNA and CAF-1 to this damaged DNA was dependent on the number of DNA lesions and required ATP. Chromatin assembly linked to the repair of single-strand breaks was disrupted by depletion of PCNA from a cell-free system. This defect was rescued by complementation with recombinant PCNA, arguing for role of PCNA in mediating chromatin assembly linked to DNA repair. We discuss the importance of the PCNA-CAF-1 interaction in the context of DNA damage processing and checkpoint control.  (+info)

Distinct importin recognition properties of histones and chromatin assembly factors. (8/113)

Synthesis of the protein components of nuclear chromatin occurs in the cytoplasm, necessitating specific import into the nucleus. Here, we report the binding affinities of the nuclear localisation sequence (NLS)-binding importin subunits for a range of histones and chromatin assembly factors. The results suggest that import of histones to the nucleus may be mediated predominantly by importin beta1, whereas the import of the other components probably relies on the conventional alpha/beta1 import pathway. Differences in recognition by importin beta1 were observed between histone H2A and the variant H2AZ, as well as between histone H3/4 with or without acetylation. The results imply that different histone variants may possess distinct nuclear import properties, with acetylation possibly playing an inhibitory role through NLS masking.  (+info)

DNA repair in the eukaryotic cell disrupts local chromatin organization. To investigate whether the resetting of nucleosomal arrays can be linked to the repair process, we developed model systems, with both Xenopus egg extract and human cell extracts, to follow repair and chromatin assembly in parallel on circular DNA templates. Both systems were able to carry out nucleotide excision repair of DNA lesions. We observed that UV-dependent DNA synthesis occurs simultaneously with chromatin assembly, strongly indicating a mechanistic coupling between the two processes. A complementation assay established that chromatin assembly factor I (CAF1) is necessary for this repair associated chromatin formation.. ...
We study proteins that regulate chromosome structure and function. We recently discovered that Chromatin Assembly Factor-1 (CAF-1) regulates protein accumulation and long-range DNA interactions at the nucleolus. (Smith et al., Mol. Biol. Cell 25: 2866-2881 (2014)). We are currently performing genome-scale analyses of the contributions of CAF-1 to human chromosome interactions and functions.
De novo chromatin assembly maintains histone density on the daughter strands in the wake of the replication fork. The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not essential for yeast cell proliferation. Depletion of CAF-1 in human cell lines demonstrated that CAF-1 was required for efficient progression through S-phase. Cells lacking CAF-1 accumulated in early and mid S-phase and replicated DNA slowly. The checkpoint kinase Chk1, but not Chk2, was phosphorylated in response to CAF-1 depletion, consistent with a DNA replication defect. CAF-1-depleted cell extracts completely lacked DNA replication-coupled chromatin assembly activity, suggesting that CAF-1 is required for efficient S-phase progression in human cells. These results indicate that, in contrast to yeast, human CAF-1 is necessary for coupling chromatin assembly with DNA replication.. ...
We have shown for the first time that the function of CAF-1 is important for viability following double-strand DNA repair. Furthermore, CAF-1 functions genetically in both homologous recombination and NHEJ, and its function during double-strand DNA repair is dependent on the interaction between CAF-1 and PCNA. Given the biochemical role of CAF-1 in DNA synthesis-coupled chromatin assembly, we propose that CAF-1 mediates chromatin assembly during double-strand DNA repair in vivo.. We have observed sensitivity of CAF-1 mutants to a variety of double-strand DNA-damaging agents. A systematic analysis of the yeast deletion collection also isolated the cac2Δ as a mutant sensitive to MMS (Chang et al. 2002). Similarly, mutants of the genes encoding Arabidopsis CAF-1, fas1, and fas2 are sensitive to MMS (Takeda et al. 2004). Given the role of CAF-1 in chromatin assembly during NER of single-strand lesions and the sensitivity of CAF-1 mutants to UV irradiation (Gaillard et al. 1996; Kaufman et al. 1997; ...
Genetic screens for the release of the TGS of Pro35S:NPTII in either the ros1 or dms3-4 mutant has identified most of the core DNA-replication proteins, including the DNA single-strand-binding protein RPA2A (Xia et al., 2006), Polα (Liu et al., 2010a), Pol ε (Yin et al., 2009), POLD2 (this study), RFC1 (Liu et al., 2010b), and TOUSLED protein kinase (Wang et al., 2007). Other studies have identified other DNA replication-related proteins involved in mediating TGS, including BRU1 (Takeda et al., 2004), chromatin assembly factor (CAF-1) subunits FAS1 and FAS2 (Ono et al., 2006; Schonrock et al., 2006), and TEBICHI (Inagaki et al., 2009). The release of TGS by defects in the DNA-replication machinery suggests that these processes must have a common mechanism. In this study, we found that POLD2 does not affect DNA methylation but plays crucial roles in regulating H3K27me3 and H3K4me3 modification according to genome-wide ChIP-seq. The released silencing of NPTII in dms3-4 is mainly due to the ...
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
Shop ATP synthase assembly factor ELISA Kit, Recombinant Protein and ATP synthase assembly factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Online ISSN 1469-3178. Copyright© 2018 EMBO. This website is best viewed using the latest versions of all modern web browsers. Older browsers may not display correctly.. ...
KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere InactivationKAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation ...
During mammalian development, chromatin dynamics and epigenetic marking are important for genome reprogramming. Recent data suggest an important role for the chromatin assembly machinery in this process. To analyze the role of chromatin assembly factor 1 (CAF-1) during pre-implantation development, we generated a mouse line carrying a targeted mutation in the gene encoding its large subunit, p150CAF-1. Loss of p150CAF-1 in homozygous mutants leads to developmental arrest at the 16-cell stage. Absence of p150CAF-1 in these embryos results in severe alterations in the nuclear organization of constitutive heterochromatin. We provide evidence that in wild-type embryos, heterochromatin domains are extensively reorganized between the two-cell and blastocyst stages. In p150CAF-1 mutant 16-cell stage embryos, the altered organization of heterochromatin displays similarities to the structure of heterochromatin in two- to four-cell stage wild-type embryos, suggesting that CAF-1 is required for the ...
Abstract: To study the relation between chromatin structure and DNA function in detail it is necessary to have an in vitro procedure for assembling nucleosomes on a naked DNA template with properties similar to native chromatin. Such procedures exist for yeast and animal model systems but have not been developed for plants. The goal of this project was to lay the groundwork for developing a chromatin assembly extract from plants. Extracts from various plant materials were tested to determine their suitability for chromatin reconstitution. Tissues from plants are thought to have much higher levels of protease and nuclease activities than those of animals or yeast. Therefore, methods to determine the relative activity of proteases and nucleases had to be developed to determine if the template DNA, histones, and chromatin assembly proteins could survive the chromatin assembly reaction. Additionally, methods to streamline the isolation of maize nuclei and purification of histones were developed. ...
Chaf1b - Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) available for purchase from OriGene - Your Gene Company.
Figure 4. CAF-1 binds (H3-H4)2 tetramers and H3-H4 dimers with similar affinities, yet 2-fold different stoichiometries. (A) An overlay of the binding curves for CAF-1 titrated into a H3(L126R, I130R)-H4 double mutant that effectively prevents tetramer formation (gray squares) and a cross-linked (H3-H4)2 tetramer (triangle) with the wild-type H3-H4 curve from Figure 1 (dashed line). The curves are very similar to wild-type and produce dissociation constants of 5.5 nM for the double mutant and 9.0 nM for the cross-linked complex (Table 1). (B) Determination of the stoichiometry of the CAF-1•WT H3-H4 complex by titration of CAF-1 into a constant concentration of labeled H3-H4. Fluorescence change occurs until wild-type H3-H4 is saturated with CAF-1 and the ratio at this inflection point is equal to the number of CAF-1 molecules bound to a single H3-H4 complex. (C) Determination of the stoichiometry of CAF-1/H3(L126R, I130R)-H4 complex. The fluorescence change plateaus similar to that of ...
Genetic information processingDNA metabolismDNA replication, recombination, and repairCDK-activating kinase assembly factor MAT1 (TIGR00570; HMM-score: 7) ...
Two main chromatin assembly pathways ensure the proper transmission of chromatin organization and chromatin-based information throughout the cell cycle. A replication-dependent (RD) pathway that couples chromatin assembly to DNA synthesis and a replication-independent (RI) pathway. Whether these pathways contribute to the establishment of chromatin domains like heterochromatin or euchromatin by introducing modifications on histones or modulating chromatin structure remains unknown. Using Xenopus laevis egg extracts we monitored RD and RI chromatin assembly on single-stranded and double-stranded DNA templates. Even though RD assembly proceeded faster than RI assembly the histone content and saturation level with nucleosomes were similar. Despite these comparable topological features, the hydrodynamic behavior of both chromatin species in sucrose gradient centrifugation clearly differed. The RD assembled chromatin ran at lower sucrose concentrations than the RI created chromatin suggesting ...
In the eukaryotic nucleus, hundreds of millions of base pairs of DNA are packed into chromosomes. Chromatin, the central nucleoprotein filament of a chromosome, has many different forms and organization levels. Chromatin is the natural state of DNA in the nucleus and the native substrate for nuclear reactions such as DNA replication, recombination, repair and transcription. The assembly of DNA into chromatin and dynamic conversion between its different forms are critical steps in the maintenance and regulation of the eukaryotic genome. The ultimate goal of our research is to understand how chromosomes are assembled and how chromatin assembly regulates the structure and activity of eukaryotic chromosomes. The crucial first step in this direction is a systematic study of factors that mediate this process. To this end we use biochemical approaches to analyze mechanisms of chromatin assembly by histone chaperones and ATP-dependent enzymes. We also dissect their function in vivo by methods of ...
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Shop ATP synthase mitochondrial F1 complex assembly factor ELISA Kit, Recombinant Protein and ATP synthase mitochondrial F1 complex assembly factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Signaling cascades are essential in instructing cell proliferation and differentiation during animal development and are often manifested by turning on or shutting off the expression of a group of genes. The specificity of different signaling pathways is usually determined by activating specific transcription factors that bind to the enhancers of their target genes. However, in eukaryotic cells the full transcription of a particular target gene is also dependent on the recruitment of chromatin remodelers and histone modifiers, in addition to transcription factors, in order to ensure a local chromatin environment that is permissive for the access of a complete set of regulatory proteins. Although CAF-1 was initially identified as a histone chaperone for DNA synthesis-coupled chromatin assembly (Smith and Stillman, 1989; Das et al., 2009), it is becoming increasingly evident that CAF-1 has functions in the regulation of other cellular and developmental processes, such as heterochromatin formation ...
Atpaf2 (untagged) - Mouse ATP synthase mitochondrial F1 complex assembly factor 2 (Atpaf2), nuclear gene encoding mitochondrial protein, (10ug), 10 µg.
A neurological exam may be performed with instruments, such as lights and reflex hammers, and usually does not cause any pain.
TY - JOUR. T1 - Histones, histone chaperones and nucleosome assembly. AU - Burgess, Rebecca J.. AU - Zhang, Zhiguo. PY - 2010/7. Y1 - 2010/7. N2 - Chromatin structure governs a number of cellular processes including DNA replication, transcription, and DNA repair. During DNA replication, chromatin structure including the basic repeating unit of chromatin, the nucleosome, is temporarily disrupted, and then reformed immediately after the passage of the replication fork. This coordinated process of nucleosome assembly during DNA replication is termed replication-coupled nucleosome assembly. Disruption of this process can lead to genome instability, a hallmark of cancer cells. Therefore, addressing how replication-coupled nucleosome assembly is regulated has been of great interest. Here, we review the current status of this growing field of interest, highlighting recent advances in understanding the regulation of this important process by the dynamic interplay of histone chaperones and histone ...
This event is part of the Condensed Matter Theory Seminar Series. Abstract: Adsorption-desorption processes are widespread in physics and biology. The 1D case for hard-exclusive particles has been studied extensively and exhibits transient jamming followed by extremely slow equilibration. However, in many realistic applications particles are somewhat compressible. We show that this profoundly alters the kinetics: the jamming plateau is replaced by a cramming phase, and the subsequent relaxation is very fast and can even be non-monotonic. As a specific example we consider the assembly kinetics of nucleosomes on eukaryotic DNA. This biological system also involves the physics of disordered systems and glassy dynamics.. ...
Intron splicing in eukaryotic organisms requires the interactions of five snRNAs and numerous different proteins in the spliceosome. Although the molecular mechanism behind splicing has been well studied, relatively little is known about regulation of expression for these splicing factor proteins. One of these proteins is the evolutionarily-conserved Drosophila RNP-4F splicing assembly factor. This protein is transcribed from a single gene into two developmentally regulated mRNAs that differ in their 5-UTR structure. In the longer isoform, known to be abundant in the developing fly central nervous system, a conserved retained intron which folds into a stem-loop has been implicated in expression control of the mRNA. Here, we describe construction and utilization of several new rnp-4f gene expression study vectors using a GFP reporter in the ΦC31 system. The results confirm our previous observation that presence of the regulatory stem-loop enhances RNP-4F protein expression. However, in that study, the
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
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The essential histone H3 variant Cse4 plays a crucial role at the centromere in S. cerevisiae, where it replaces histone H3 in that it assembles centromere specific (Cse4-H4)2 tetrameres. We found in our study that the histone H3 variant was able to interact over its unique N-Terminus with two subunits of the histone acetyltransferase complex SAS-I: Sas2 and Sas4. Mutations within the acetyl-CoA binding site (HAT domain) or the zink-finger of Sas2 disrupted the binding to Cse4, although an indirect interaction was found with co-immunoprecipitation experiments. Additionally, the N-terminus of Cse4 interacted with Cac1, the largest subunit of the chromatin assembly factor CAF-I and Asf1 - two histone chaperones that assemble histones H3 and H4 into nucleosomes. Our findings further suggest a role of Cac1 independent of Cac2 and Cac3 as no binding to Cse4 could be detected. A role for Sas2 at the centromere was further confirmed in that a sas2 deletion (sas2 delta) disrupted the binding of Cse4 to ...
Stuffing the long strands of chromosomal DNA into a eukaryotic nucleus requires that the DNA be compacted in length approximately 10,000 to 50,000 -fold. Incredibly, cells achieve this tight packing of the DNA while still maintaining the chromosomes in a form that allows regulatory proteins to gain access to the DNA to turn on (or off) specific genes or to duplicate the chromosomal DNA (replication). This engineering feat is accomplished by a variety of chromatin proteins, the most abundant of which are the histones. Histones associate with DNA to accomplish the first step in chromatin assembly, forming protein-DNA structures known as nucleosomes. When viewed under the electron microscope, nucleosomes are beads of ~10 nm in diameter that are distributed along the ~2 nm DNA string (Kornberg, 1974; Olins and Olins, 1974) about once every 200 bp. Each bead is a nucleosome core particle (install the plug-in to view this link) that includes ~146 bp of DNA wrapped almost twice around a core histone ...
The anti-silencing function protein 1 (Asf1) is a chaperone that forms a complex with histones H3 and H4 facilitating dimer deposition and removal ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Complete information for ASF1A gene (Protein Coding), Anti-Silencing Function 1A Histone Chaperone, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
yn ystod Blwyddyn 1, 2 ar Flwyddyn Dramor syn berthnasol o ran astudio Ieithoedd a Diwylliannau Modern. O gwblhau traethawd hir yn llwyddiannus maer myfyrwyr yn cael cyfle i weithio ar brosiect ymchwil fel rhan or cwrs gradd ar bwnc syn ymwneud â chymdeithas, hanes, llenyddiaeth a diwylliant y wlad berthnasol nad yw eisoes yn rhan benodol or maes llafur. Dylid penderfynu ar y pwnc mewn trafodaeth âr Goruchwyliwr. Bwriad y modiwl hwn yw bod yn sylfaen ir math o ysgrifennu syn ofynnol ar lefel ôl-raddedig.. Bydd y modiwl hwn yn galluogir myfyriwr 1. I gasglu rhestr o ffynonellau perthnasol ar gyfer y pwnc a ddewiswyd ganddynt 2. I gasglu deunydd ymchwil eilaidd perthnasol ar gyfer y pwnc a ddewiswyd ganddynt. 3. I gasglu deunydd ymchwil gwreiddiol perthnasol ar gyfer y pwnc a ddewiswyd ganddynt. 4. I archwilio a dadansoddi eu deunydd ffynhonnell mewn perthynas â chwestiynau ymchwil perthnasol. 5. I lunio cynllun penodau cydlynol ar gyfer eu traethawd hir. 6. I ysgrifennu adroddiad ...
RBBP7 Full-Length MS Protein Standard (NP_002884), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
Kinderparacetamol SDG is a medicine available in a number of countries worldwide. A list of US medications equivalent to Kinderparacetamol SDG is available on the Drugs.com website.
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5 ...
Previous work has revealed important roles for HIRA in myogenesis. It is important for the differentiation of C2C12 myoblasts into myotubes (Song et al., 2012). HIRA facilitates expression of Myod1 through incorporation of histone H3.3 into its promoter and enhancer regions (Yang et al., 2011b). Histone H3.3 itself epigenetically maintains expression of the Myod1 gene after cell division (Ng and Gurdon, 2008). HIRA cooperates with MEF2 transcription factors to drive expression of MEF2 target genes, like Myog (Yang et al., 2011a). HIRA is also a substrate for AKT proteins. HIRA phosphorylation by AKT proteins in myoblasts preserves the proliferative state. Dephosphorylation occurs as myoblasts differentiate towards myotubes (Yang et al., 2016). Thus, HIRA-mediated chromatin assembly is critical during myogenesis. In this study, we expanded our understanding of the function of HIRA in skeletal muscle by determining the consequence of its loss after myogenesis in the homeostasis and function of ...
Taken together, these studies will reveal how the transcriptional machinery competes with chromatin assembly to regulate transcription, not only during genome activation but potentially also during differentiation and reprogramming.. 2. The role of chromatin structure and nuclear architecture in genome activation. Chromatin regulates the accessibility of the genome for DNA binding proteins. Changes in chromatin structure and nuclear organization are thus critical to understanding how regions of the genome become transcriptionally competent. We have previously shown that dramatic changes in chromatin structure accompany the onset of zygotic gene expression (Vastenhouw et al., Nature 2010 PMID: 20336069; Zhang et al., GR 2014 PMID: 24285721). Thus, the onset of transcription during genome activation provides a powerful system to study the relationship between chromatin structure and transcriptional activity.. We have recently found that transcriptional activity and accumulation of the produced RNA ...
Booth DG, Beckett AJ, Molina O, Samejima I, Masumoto H, Kouprina N, Larionov V, Prior IA, Earnshaw WC. 3D-CLEM Reveals that a Major Portion of Mitotic Chromosomes Is Not Chromatin. Mol Cell. 2016 Nov 17;64(4):790-802. doi: 10.1016/j.molcel.2016.10.009. Epub 2016 Nov 10. PMID: 27840028; FREE ARTICLE. Ohzeki J, Shono N, Otake K, Martins NM, Kugou K, Kimura H, Nagase T, Larionov V, Earnshaw WC, Masumoto H. KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation. Dev Cell. 2016 Jun 6;37(5):413-27. doi: 10.1016/j.devcel.2016.05.006. PMID: 27270040; FREE ARTICLE. Ohta S, Montaño-Gutierrez LF, de Lima Alves F, Ogawa H, Toramoto I, Sato N, Morrison CG, Takeda S, Hudson DF, Rappsilber J, Earnshaw WC. Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes. Mol Cell Proteomics. 2016 Aug;15(8):2802-18. doi: 10.1074/mcp.M116.057885. Epub 2016 May 26. PMID: 27231315; ...
This guide will provide you with all of the information you need on the acronym ASF, including its definition, usage, examples of ASF, and more!
The centromeric histone CENP-A is incorporated at different cell cycle phases during somatic mitosis, meiosis I and meiosis II in Drosophila melanogaster.
The chromo domain was originally identified as a protein sequence motif common to the Drosophila chromatin proteins, Polycomb (Pc) and heterochromatin protein 1 [HP1; Paro and Hogness (1991) Proc. Natl. Acad. Sci. USA, 88, 263-267; Paro (1990) Trends Genet., 6, 416-421]. Here we describe a second chromo domain-like motif in HP1. Subsequent refined searches identified further examples of this chromo domain variant which all occur in proteins that also have an N-terminally located chromo domain. Due to its relatedness to the chromo domain, and its occurrence in proteins that also have a classical chromo domain, we call the variant the chromo shadow domain. Chromo domain-containing proteins can therefore be divided into two classes depending on the presence, for example in HP1, or absence, for example in Pc, of the chromo shadow domain. We have also found examples of proteins which have two classical chromo domains. The Schizosaccharomyces pombe SWI6 protein, involved in repression of the silent ...
Create a Notification; as soon as the NotificationJobs finds some NotificationTask to execute, execution gets persisted but NotificationTask.getExecs() is empty.. Possibly related to ...
Chromatin assembly factor 1 subunit B is a protein that in humans is encoded by the CHAF1B gene. Chromatin assembly factor I ( ... "Entrez Gene: CHAF1B chromatin assembly factor 1, subunit B (p60)". Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, ... Nabatiyan A, Krude T (Apr 2004). "Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of ... Shim KS, Bergelson JM, Furuse M, Ovod V, Krude T, Lubec G (2004). "Reduction of chromatin assembly factor 1 p60 and C21orf2 ...
"Functional analysis of the subunits of the chromatin assembly factor RSF". Mol Cell Biol. 23 (19): 6759-68. doi:10.1128/MCB. ... Remodeling and spacing factor 1 is a protein that in humans is encoded by the RSF1 gene. HBXAP is involved in transcription ... "Entrez Gene: RSF1 remodeling and spacing factor 1". Lee TH, Elledge SJ, Butel JS (1995). "Hepatitis B virus X protein interacts ... LeRoy G, Orphanides G, Lane WS, Reinberg D (1998). "Requirement of RSF and FACT for transcription of chromatin templates in ...
Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068- ... are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM] ... Sen SP, De Benedetti A (2006). "TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1". BMC Molecular ...
Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene. Chromatin assembly factor I ( ... "Entrez Gene: CHAF1A chromatin assembly factor 1, subunit A (p150)". Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, ... Kaufman PD, Kobayashi R, Kessler N, Stillman B (August 1995). "The p150 and p60 subunits of chromatin assembly factor I: a ... interaction between chromatin assembly factor 1 and HP1 proteins". Mol. Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00)80204-X ...
"Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function". Molecular and ... Apoptotic factors ATPases Base excision repair enzymes Cell-cycle regulators Chromatin remodeling factor Clamp loader Cohesin ... PCNA is clamped to DNA through the action of replication factor C (RFC), which is a heteropentameric member of the AAA+ class ... Missing or empty ,title= (help) Miura M (1999). "Detection of chromatin-bound PCNA in mammalian cells and its use to study DNA ...
"Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1". Mol. Cell. 7 (1): 13-20. doi: ... cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in ... Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene. The protein encoded by ... Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue". J. Biol. Chem. 267 (33): ...
Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. These are different ... 202 (1-2): 193-201. doi:10.1016/S0378-1119(97)00495-2. PMID 9427565. Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA ( ...
Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Transcriptional regulation of human chromatin assembly factor ASF1". DNA and Cell Biology. 26 (2): 91-9. doi:10.1089/dna. ... in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. ... Sen SP, De Benedetti A (2006). "TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1". BMC Molecular ...
It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair ... Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068- ... Munakata T, Adachi N, Yokoyama N, Kuzuhara T, Horikoshi M (Mar 2000). "A human homologue of yeast anti-silencing factor has ...
... interaction between chromatin assembly factor 1 and HP1 proteins". Molecular Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00) ... a potential role in the regulation of higher-order chromatin structure". The Journal of Pathology. 196 (2): 135-44. doi:10.1002 ... Chromobox protein homolog 1 is a protein that in humans is encoded by the CBX1 gene. The protein is localized at ... 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015. van der Weyden L, White JK, Adams DJ, Logan DW ( ...
... interaction between chromatin assembly factor 1 and HP1 proteins". Molecular Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00) ... "The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1". Molecular and Cellular ... "Selective interaction between the chromatin-remodeling factor BRG1 and the heterochromatin-associated protein HP1alpha". The ... "Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis". ...
"The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 23 (9): ... "The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 23 (9): ... Assembly of novel secondary chromatin structures in the absence of DNA methylation". J. Biol. Chem. 278 (34): 32181-8. doi: ... Ueba T, Kaspar B, Zhao X, Gage FH (1999). "Repression of human fibroblast growth factor 2 by a novel transcription factor". J. ...
Nair SS, Li DQ, Kumar R (February 2013). "A core chromatin remodeling factor instructs global chromatin signaling through ... "G9a inhibits MEF2C activity to control sarcomere assembly". Scientific Reports. 6 (1): 34163. Bibcode:2016NatSR...634163O. doi: ... For example, G9A/GLP can methylate a number of transcription factors to regulate their transcriptional activity, including MyoD ... January 2012). "Lysine methyltransferase G9a methylates the transcription factor MyoD and regulates skeletal muscle ...
Proteins RbAp48 is a key player in the assembly of nucleosomes. RbAp48 protein is a subunit of the chromatin-assembly factor-1 ... a human cell factor required for chromatin assembly during DNA replication in vitro". Cell. 58 (1): 15-25. doi:10.1016/0092- ... Chromatin function and acetylation A direct link between chromatin function and acetylation was established by the discovery ... interacts with H3-H4 dimers and imply that the function of RbAp48 is involved in numerous process such as chromatin assembly, ...
... and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1". ... 256 (1): 225-36. doi:10.1006/excr.1999.4778. PMID 10739669. Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, ... 75 (1): 104-8. doi:10.1002/(SICI)1096-8628(19980106)75:1. 3.0.CO;2-P. PMID 9450867. "Entrez Gene: TOP3A topoisomerase (DNA) III ...
... and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1". ... 46 (1): 43-53. doi:10.1016/j.molcel.2012.02.020. PMC 3328772. PMID 22500736. Séguéla-Arnaud M, Crismani W, Larchevêque C, Mazel ... Sharma S, Sommers JA, Wu L, Bohr VA, Hickson ID, Brosh RM (March 2004). "Stimulation of flap endonuclease-1 by the Bloom's ... doi:10.1016/0092-8674(95)90105-1. PMID 7585968. S2CID 13439128. German J, Roe AM, Leppert MF, Ellis NA (1994). "Bloom syndrome ...
The heterotrimeric chaperone chromatin assembly factor 1 (CAF-1) is a chromatin formation protein that is involved in ... "Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function". Molecular and ... The chromatin licensing and DNA replication factor 1 (Cdt1) protein is required for the licensing of chromatin for DNA ... The assembly of the Mcm proteins onto chromatin requires the coordinated function of the origin recognition complex (ORC), Cdc6 ...
"The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating ... Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in ... "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. ... 327 (1): 111-28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612. Jiang W, McDonald D, Hope TJ, Hunter T (Oct 1999). " ...
"The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating ... After chromatin undergoes changes in telophase of mitosis, the hexameric protein complex of MCM proteins 2-7 forms part of the ... Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in ... Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast ...
Makde R, England J, Yennawar H, Tan S (2010). "Structure of the RCC1 chromatin factor bound to the nucleosome core particle". ... The gradient triggers release of spindle assembly factors (SAFs) from inhibitory interactions via the transport proteins ... and yeast are required for proper spindle assembly; in mammals, CLASP1 and CLASP2 both contribute to proper spindle assembly ... Thus, a gradient of GTP-bound Ran is generated around the vicinity of mitotic chromatin. Glass beads coated with RCC1 induce ...
ACF (for ATP-utilising chromatin assembly and remodeling factor) is a chromatin-remodeling complex that catalyzes the ATP- ... ATP-dependent chromatin assembly factor large subunit Acf1 human - WSTF (Williams syndrome transcription factor) mouse - cbp146 ... "Binding of Acf1 to DNA involves a WAC motif and is important for ACF-mediated chromatin assembly". Mol. Cell. Biol. 22 (18): ... that function cooperatively in the ATP-dependent catalysis of chromatin assembly". Genes Dev. 13 (12): 1529-39. doi:10.1101/gad ...
... to study DNA replication-coupled chromatin assembly in a test tube and discovered proteins such as Chromatin Assembly Factor-1 ... The proteins that combine with DNA to organize the genome into a chromatin structure include histones. He developed a ... Waga, S.; Bauer, G.; Stillman B. (1994). "Reconstitution of complete SV40 DNA replication with purified replication factors". ... These studies resulted in understanding how chromatin is inherited. Commonwealth Postgraduate Award (1976-1978) Damon Runyon- ...
... chromatin assembly factor-1), a protein complex maintaining chromatin Conductive anodic filament, an electrochemical migration- ... by ISO 3166-1 country code Common Assessment Framework, an EU tool to bring quality improvements to public sector services ...
Four transcription factor-like proteins have been found in PBSV-1, including TFIIB (A107L), TFIID (A552R), TFIIS (A125L), and a ... The second phase of proteins are translated in the cytoplasm and the assembly of virus capsids begins about 2-3 hours post ... The HaV virus, infecting H. akashiwo has been shown to be a factor in bloom termination. Suttle et al. (1990) suggested that ... The eukaryotic replication factor C". European Journal of Biochemistry. 254 (2): 209-216. doi:10.1046/j.1432-1327.1998.2540209. ...
CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. It is a ... The protein encoded by this gene is a key licensing factor in the assembly of pre-replication complexes (pre-RC), which occurs ... "Entrez Gene: CDT1 chromatin licensing and DNA replication factor 1". Reference, Genetics Home. "CDT1 gene". Genetics Home ... In the assembly of pre-RCs, origin recognition complexes (ORC1-6) recognize and bind to DNA replication origins. CDT1, along ...
Kaufman PD, Kobayashi R, Kessler N, Stillman B (June 1995). "The p150 and p60 subunits of chromatin assembly factor I: a ... They are assembled by the chromatin assembly factor-1 (CAF-1) complex, which consists of three subunits (p150, p60, and p48). ... Yadav T, Whitehouse I (April 2016). "Replication-Coupled Nucleosome Assembly and Positioning by ATP-Dependent Chromatin- ... Nucleosome core particles are observed when chromatin in interphase is treated to cause the chromatin to unfold partially. The ...
Protocol for in vitro Chromatin Assembly ENCODE threads Explorer Chromatin patterns at transcription factor binding sites. ... Other factors are probably involved, such as the association and dissociation of transcription factor complexes with chromatin ... In eukaryotes, ATP-dependent chromatin remodeling complexes and histone-modifying enzymes are two predominant factors employed ... When the chromatin decondenses, the DNA is open to entry of molecular machinery. Fluctuations between open and closed chromatin ...
... major roles in chromatin decondensation and nuclear assembly". J. Cell Biol. 158 (3): 475-85. doi:10.1083/jcb.200202019. PMC ... Barrier-to-autointegration factor is a protein that in humans is encoded by the BANF1 gene. It is a member of the barrier-to- ... Barrier to autointegration factor 1 has been shown to interact with Thymopoietin. Mutations in this gene have been shown to ... 2006). "Cellular co-factors of HIV-1 integration". Trends Biochem. Sci. 31 (2): 98-105. doi:10.1016/j.tibs.2005.12.002. PMID ...
Chromatin assembly factor-1) - histone chaperone that execute a coordinating role in сhromatin remodeling. Teif VB, Rippe K ( ... The ISWI-family remodelers have been shown to play central roles in chromatin assembly after DNA replication and maintenance of ... Chromatin remodeling is the dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the ... Epigenetics Histone Nucleosomes Chromatin Histone acetyltransferase Transcription factors CAF-1 ( ...
Chromatin assembly factor-1 (CAF-1) is a protein complex - including Chaf1a (p150), Chaf1b (p60), and p50 subunits - that ... Kaufman PD, Kobayashi R, Kessler N, Stillman B (1995). "The p150 and p60 subunits of chromatin assembly factor I: a molecular ... Smith S, Stillman B (1989). "Purification and characterization of CAF-I, a human cell factor required for chromatin assembly ... Hoek, M.; Stillman, B. (2003). "Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in ...
Henriques AO, Moran CP Jr (2007). "Structure, assembly, and function of the spore surface layers". Annu Rev Microbiol. 61: 555- ... The core contains the spore chromosomal DNA which is encased in chromatin-like proteins known as SASPs (small acid-soluble ... The heat resistance of endospores is due to a variety of factors: *Calcium dipicolinate, abundant within the endospore, may ... These studies have contributed much to our understanding of the regulation of gene expression, transcription factors, and the ...
chromatin. • condensed chromosome. • nuclear chromosome, telomeric region. • nucleus. • nuclear chromatin. • lateral element. • ... DNA recombinase assembly. • meiotic cell cycle. • negative regulation of G0 to G1 transition. ... Sigurdsson S, Van Komen S, Petukhova G, Sung P (Nov 2002). "Homologous DNA pairing by human recombination factors Rad51 and ... chromatin binding. • DNA polymerase binding. • protein C-terminus binding. • protein binding. • four-way junction DNA binding. ...
"Human Genome Assembly GRCh38 - Genome Reference Consortium". National Center for Biotechnology Information. 2013-12-24. ... PMFBP1: encoding protein Polyamine-modulated factor 1-binding protein 1. *POLR3K: encoding enzyme DNA-directed RNA polymerase ... Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). Last update 2014-06-03. Retrieved 2017-04-26. ... Ideogram data for Homo sapience (400 bphs, Assembly GRCh38.p3). Last update 2014-03-04. Retrieved 2017-04-26. ...
Additional transcription factors then bind, first TFIIE and then TFIIH.[24] This completes the assembly of the preinitiation ... Other factors must stimulate the BTC to increase transcription levels.[2] One such example of a BTC stimulating region of DNA ... Gastric cancer is correlated with TATA box polymorphism.[39] The TATA box has a binding site for the transcription factor of ... The TATA box is the binding site of the TATA-binding protein (TBP) and other transcription factors in some eukaryotic genes. ...
Ahmad K, Henikoff S (Jun 2002). "The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly ... Increased chromatin compaction prevents transcription factors from accessing DNA, and reduces the likelihood of new ... Chromatin regulation[edit]. Histones undergo posttranslational modifications that alter their interaction with DNA and nuclear ... These minor histones usually carry out specific functions of the chromatin metabolism. For example, histone H3-like CENPA is ...
Genome assembly and validation; mathematical modeling and algorithm development; population genetics; applied statistical and ... Waga, S; Bauer, G; Stillman, B (April 1994). "Reconstitution of complete SV40 DNA replication with purified replication factors ... chromatin dynamics; structural biology; advanced proteomics; mass spectrometry; advanced microscopy. ... 1 in the world.[5] The Laboratory is led by Bruce Stillman, a biochemist and cancer researcher. ...
activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ... ribosome assembly. • intracellular protein transport. • reparo de ADN. • transdução de sinal. • negative regulation of cell ... nucleosome assembly. • viral process. • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle ... CENP-A containing nucleosome assembly. • positive regulation of transcription, DNA-templated. • cell aging. • positive ...
Human chromatin-associated protein LEDGF, which tightly binds HIV IN and directs HIV PIC towards highly expressed genes for ... Hare S, Gupta SS, Valkov E, Engelman A, Cherepanov P (March 2010). "Retroviral intasome assembly and inhibition of DNA strand ... integration, is an example of such a host factor. Integration occurs following production of the double-stranded viral DNA by ... Antiretroviral effect of MK-0518, a novel HIV-1 integrase inhibitor, in ART-naïve HIV-1 infected patients. Program and ...
He considered social factors such as poverty major causes of disease.[103] He even attacked Koch's and Ignaz Semmelweis' policy ... He coined biological terms such as "chromatin", "neuroglia", "agenesis", "parenchyma", "osteoid", "amyloid degeneration", and " ... A state funeral was held on 9 September in the Assembly Room of the Magistracy in the Berlin Town Hall, which was decorated ... Related to this research, Virchow described the factors contributing to venous thrombosis, Virchow's triad.[22][58] ...
Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF)[1] are autoantibodies that bind to contents of the cell ... CENP-C is a 140kDa protein involved in kinetochore assembly. CENP-D is a 50kDa protein of unknown function, but may be ... The homogeneous pattern is seen when the condensed chromosomes and interphase chromatin stain. This pattern is associated with ... The La antigen is a 48kDa transcription termination factor of RNA polymerase III, which associates with the Ro-RNP complex.[15] ...
Single cell transposes-accessible chromatin sequencing maps chromatin accessibility across the genome. A transposase inserts ... Single-cell analysis of the several transcription factors by scRNA-seq revealed heterogeneity across the population. These ... Some of the genome assembly tools that can be used in single cell genome sequencing include: SPAdes, IDBA-UD, Cortex and HyDA.[ ... Single-cell assay for transposase-accessible chromatin with sequencing (scATAC-seq)[edit]. Main article: ATAC-seq ...
"Mitotic bookmarking by transcription factors". Epigenetics & Chromatin. 6 (1): 6. doi:10.1186/1756-8935-6-6. PMC 3621617. PMID ... Zhou J, Yao J, Joshi HC (September 2002). "Attachment and tension in the spindle assembly checkpoint". Journal of Cell Science ... The cell undergoes mitotic cell rounding during spindle assembly and then divides via cytokinesis. The actomyosin cortex is ... During interphase, the genetic material in the nucleus consists of loosely packed chromatin. At the onset of prophase, ...
Boulikas T (1995). "Phosphorylation of transcription factors and control of the cell cycle". Crit Rev Eukaryot Gene Expr. 5 (1 ... Stuurman N, Heins S, Aebi U (1998). "Nuclear lamins: their structure, assembly, and interactions". J Struct Biol. 122 (1-2): 42 ... Ehrenhofer-Murray A (2004). "Chromatin dynamics at DNA replication, transcription and repair". Eur J Biochem. 271 (12): 2335- ... 2000). "Nuclear Lamins A and B1: Different Pathways of Assembly during Nuclear Envelope Formation in Living Cells". Journal of ...
... forming a material called chromatin; in eukaryotes, chromatin is usually composed of nucleosomes, segments of DNA wound around ... Transcription factors are regulatory proteins that bind to DNA, either promoting or inhibiting the transcription of a gene.[72] ... Assembly of a Contiguous DNA Sequence Archived 8 February 2007 at the Wayback Machine ... such tests cannot separate genetic factors from environmental factors affecting fetal development. ...
Holmquist GP, Ashley T (2006). "Chromosome organization and chromatin modification: influence on genome function and evolution ... including transcription factors as well as F-box proteins.[99] In many organisms, including humans, miRNAs are linked to the ... "Cleavage of the siRNA passenger strand during RISC assembly in human cells". EMBO Reports. 7 (3): 314-20. doi:10.1038/sj.embor ... 10 (1): 1-12. doi:10.1016/j.ajps.2014.08.011.. *^ a b c d e f g h i j k l m Whitehead KA, Dahlman JE, Langer RS, Anderson DG ( ...
... and the general transcription factors TFIIE and TFIIF.[4] Other proteins include CIITA, Brm (chromatin remodeler), NF-κB (p65 ... Type B HATs are located in the cytoplasm and are responsible for acetylating newly synthesized histones prior to their assembly ... Chromatin remodeling[edit]. Histone acetyltransferases serve many biological roles inside the cell. Chromatin is a combination ... and the general transcription factors TFIIE and TFIIF.[4] Other substrates include the transcription factors Sp1, KLF5, FOXO1, ...
Schwarz DS, Hutvágner G, Du T, Xu Z, Aronin N, Zamore PD (2003). "Asymmetry in the assembly of the RNAi enzyme complex". Cell ... Holmquist G, Ashley T (2006). "Chromosome organization and chromatin modification: influence on genome function and evolution ... a proteína R2D2 pode servir como o factor de diferenciación ao unirse o extremo 5', máis estable, da cadea pasaxeira.[29] ... Matranga C, Tomari Y, Shin C, Bartel D, Zamore P (2005). "Passenger-strand cleavage facilitates assembly of siRNA into Ago2- ...
"Human Genome Assembly GRCh38 - Genome Reference Consortium". National Center for Biotechnology Information. 2013-12-24. ... SOX2: transcription factor. *SOX2OT: SOX2 overlapping transcript. *SPG14 encoding protein Spastic paraplegia 14 (autosomal ... Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). Last update 2014-06-03. Retrieved 2017-04-26. ... Ideogram data for Homo sapience (400 bphs, Assembly GRCh38.p3). Last update 2014-03-04. Retrieved 2017-04-26. ...
transcription factor activity, sequence-specific DNA binding. • transcription coactivator activity. • zinc ion binding. • ... cell-cell junction assembly. • toll-like receptor 9 signaling pathway. • negative regulation of monocyte chemotactic protein-1 ... "BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation" ... transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding. • chenodeoxycholic acid binding. • ...
ORF18, ORF24, ORF30, ORF31, ORF34, ORF66 - viral transcription factors required for the expression of late genes ... inhibits p53 and retinoblastoma protein and suppresses viral genes needed for full virus production and assembly ("lytic ... K8 - transcriptional repressor - modulates chromatin K8.1 - envelope glycoprotein K9 - vIRF1, viral interferon regulatory ... ORF50 - RTA, replication and transcription activator - the major transcription factor driving lytic KSHV reactivation ...
SWI/SNF is a chromatin remodeling complex. Artificial tethering of BRCA1 to chromatin was shown to decondense heterochromatin, ... cellular response to tumor necrosis factor. • positive regulation of DNA repair. • fatty acid metabolic process. • positive ... VCP is recruited to DNA lesions and cooperates with the ubiquitin ligase RNF8 to orchestrate assembly of signaling complexes ... 1 (1): e201204005. doi:10.5936/csbj.201204005. PMC 3380633. PMID 22737296.. *^ a b c d Brzovic PS, Rajagopal P, Hoyt DW, King ...
"Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln". Curr. Biol. 11 (24): ... spliceosomal snRNP assembly. • liver regeneration. • methylation. • peptidyl-arginine methylation. • regulation of ERK1 and ... chromatin organization. • histone arginine methylation. Sources:Amigo / QuickGO. Orthologs. Species. Human. Mouse. ... doi:10.1016/s1097-2765(03)00101-1. PMID 12718890.. *^ Guderian G, Peter C, Wiesner J, Sickmann A, Schulze-Osthoff K, Fischer U ...
There has been evidence that these HDACs also interact with HDAC3 as a co-recruitment factor to the SMRT/N-CoR factors in the ... Histone acetylation alters chromatin structure. Shown in this illustration, the dynamic state of histone acetylation/ ... allowing histones to interact with DNA more tightly to form compacted nucleosome assembly. This increase in the rigid structure ... myocyte enhancer factor 2 (MEF2) and 14-3-3.[5][6] All three HDACs work to repress the myogenic transcription factor MEF2 which ...
RNA polymerase II repressing transcription factor binding. • protein binding. • recombinase activity. • chromatin binding. • ... Nam Y, Weng AP, Aster JC, Blacklow SC (Jun 2003). "Structural requirements for assembly of the CSL.intracellular Notch1. ... transcription factor activity, sequence-specific DNA binding. • sequence-specific DNA binding. • transcription factor binding. ... repressing transcription factor binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
Assembly and disassembly. An image of a newt lung cell stained with fluorescent dyes during metaphase. The mitotic spindle can ... During most of the cell cycle these are organized in a DNA-protein complex known as chromatin, and during cell division the ... There they serve as transcription factors when bound to their ligand; in the absence of a ligand, many such receptors function ... The best-known of these is the nucleolus, which is mainly involved in the assembly of ribosomes. After being produced in the ...
Ito T (2003). "Nucleosome assembly and remodeling". Current Topics in Microbiology and Immunology. 274: 1-22. doi:10.1007/978-3 ... It may act as a recognition factor to regulate the attachment and dispersal of specific cell types in the biofilm;[102] it may ... Further information: Cell nucleus, Chromatin, Chromosome, Gene, and Noncoding DNA. Genomic DNA is tightly and orderly packed in ... Each transcription factor binds to one particular set of DNA sequences and activates or inhibits the transcription of genes ...
transcription factor complex. • spindle. • cyclin/CDK positive transcription elongation factor complex. • chromatin. • cell ... The TFIID binds to the TATA box in order to begin the assembly of the TFIIA, recruiting other transcription factors and ... transcription factor binding. • RNA polymerase II activating transcription factor binding. • phosphoprotein binding. • kinase ... protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis. ...
Kauzmann W (1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. Supplement. 47 (Suppl 1): 113- ... and allow the assembly of large protein complexes that carry out many closely related reactions with a common biological ... Chromatin immunoprecipitation. *Surface plasmon resonance. *Isothermal titration calorimetry. *X-ray crystallography. *Protein ... Kauzmann W (1959). "Some factors in the interpretation of protein denaturation". Advances in Protein Chemistry. Advances in ...
... mostly by interfering with general transcription factor (GTF) assembly.[2] NREs passively repress the gene, usually by ... The best example of this is the Neuronal-Restrictive Silencer Factor (NRSF) that is produced by the REST gene. The REST gene ... In genetics, a silencer is a DNA sequence capable of binding transcription regulation factors, called repressors. DNA contains ... The TATA box binding proteins also include the transcription factor II B (TFIIB) which binds to both DNA and RNA polymerases.[6 ...
Ye, Q; Hu Y F, Zhong H, Nye A C, Belmont A S, Li R (2001). «BRCA1-induced large-scale chromatin unfolding and allele-specific ... cellular response to tumor necrosis factor. • positive regulation of DNA repair. • fatty acid metabolic process. • positive ... Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex». Proc. Natl. Acad. Sci. U.S.A. 100 ( ... Neish, A S; Anderson S F, Schlegel B P, Wei W, Parvin J D (1998). «Factors associated with the mammalian RNA polymerase II ...
Chromatin assembly factor 1 subunit A (CAF1 subunit A or subunit p150) is a core component of the CAF-1 complex, a complex ... Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in ... The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.. Mol. Cell. Biol. 23 3226- ... Chromatin assembly factor 1 subunit A, C-terminal (IPR029105). Short name: CAF1-p150_C2 ...
Chromatin assembly factor-1 (CAF-1) is a protein complex - including Chaf1a (p150), Chaf1b (p60), and p50 subunits - that ... Kaufman PD, Kobayashi R, Kessler N, Stillman B (1995). "The p150 and p60 subunits of chromatin assembly factor I: a molecular ... Smith S, Stillman B (1989). "Purification and characterization of CAF-I, a human cell factor required for chromatin assembly ... Hoek, M.; Stillman, B. (2003). "Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in ...
Chromatin assembly factor 1, subunit BImported. ,p>Information which has been imported from another database using automatic ... tr,H3BYY5,H3BYY5_TETNG Chromatin assembly factor 1, subunit B OS=Tetraodon nigroviridis OX=99883 PE=4 SV=1 ... SSF50978 SSF50978, 1 hit. PROSITEi. View protein in PROSITE. PS00678 WD_REPEATS_1, 1 hit. PS50082 WD_REPEATS_2, 3 hits. ... H3BYY5-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MKVVTCEIAW HNKEPVYSLD FQHGCDGRLH RLATAGVDTA ...
... and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon ... Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto ... histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. UniProt ... Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of ...
Compare chromatin assembly factor 1 subunit B ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... chromatin assembly factor 1 subunit B ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ... Your search returned 47 chromatin assembly factor 1 subunit B ELISA ELISA Kit across 6 suppliers. ... THP-1 cells were treated with LPS for 24 hours and treated with a TAK1 inhibitor. The ELISA kit was ... read more ...
... a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating ... histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A ... CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating ... Chromatin assembly factor 1 subunit AAdd BLAST. 956. Amino acid modifications. Feature key. Position(s). DescriptionActions. ...
1996 Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I. Cell 86: 887-896. ... Kaufman, P. D., R. Kobayashi, N. Kessler and B. Stillman, 1995 The p150 and p60 subunits of chromatin assembly factor I: a ... Myung, K., V. Pennaneach, E. S. Kats and R. D. Kolodner, 2003 Saccharomyces cerevisiae chromatin-assembly factors that act ... Prado, F., F. Cortes-Ledesma and A. Aguilera, 2004 The absence of the yeast chromatin assembly factor Asf1 increases genomic ...
Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ...
Post-replicative chromatin assembly by Drosophila and human chromatin assembly factor-1.Mol. Cell. Biol. 16 1996 810 817 ... Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I.Cell 86 1996 887 896 ... RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo.Genes Dev. 11 1997 ... such as ATP-utilizing chromatin assembly and remodeling factor (ACF), which catalyzes the assembly of histones into periodic ...
What is chromatin assembly factor 1 subunit B? Meaning of chromatin assembly factor 1 subunit B medical term. What does ... Looking for online definition of chromatin assembly factor 1 subunit B in the Medical Dictionary? chromatin assembly factor 1 ... which is thought to mediate chromatin assembly in DNA replication and DNA repair. Chromatin assembly factor 1, subunit B, is a ... redirected from chromatin assembly factor 1 subunit B) CHAF1B. A gene on chromosome 21q22.13 that encodes chromatin assembly ...
Chromatin assembly factor 1 (CAF1), a histone chaperone that mediates the deposition of histone H3/H4 onto newly synthesized ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ...
The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein.. J K Tyler ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ...
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Mouse Monoclonal Antibody Specific to CAF-1 (1SS-48). Use: IF, IP, WB. Function: Cell Div. Cycle/Mitosis, Gene Reg. & RNA ... DESCRIPTION: Chromatin assembly factor-1 (CAF-1) is a multi protein complex that functions to assemble nucleosomes in a ... Chromatin Assembly Factor 1 (CAF-1; Clone 1SS-48). Mouse Monoclonal Antibody. PRODUCT #: 45-6795 ... The CAF-1 complex is composed of three subunits, p150, p60 and p48 in stoichiometric amounts. The composition of CAF-1 is ...
Chromatin Assembly Factor 1 Subunit A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Chromatin assembly factor I p150 subunit (CAF1A_HUMAN). *Chromatin assembly factor 1, subunit A (P150), isoform CRA_a (D6W625_ ... Chromatin assembly factor 1 subunit A. Protein Accession:. Q13111. Secondary Accessions: *Q6NXG5 ... CHAF1A (Chromatin Assembly Factor 1 Subunit A) is a Protein Coding gene. Among its related pathways are Autophagy Pathway and ...
Chromatin Assembly Factor 1 Subunit A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Chromatin assembly factor I p150 subunit (CAF1A_HUMAN). *Chromatin assembly factor 1, subunit A (P150), isoform CRA_a (D6W625_ ... Chromatin assembly factor 1 subunit A. Protein Accession:. Q13111. Secondary Accessions: *Q6NXG5 ... Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene. (PMID: 11250073) Dong H … Huang GM ( ...
... a physiological nucleosome assembly factor. EMBO J. 3, 1193-1199.PubMedGoogle Scholar ... RYOJI, M. and WORCEL, A. (1984). Chromatin assembly in Xenopus oocytes: in vivo studies. Cell 37, 21-32.PubMedCrossRefGoogle ... GLIKIN, G.C., RUBERTI, I., WORCEL, A. (1984). Chromatin assembly in Xenopus oocytes: in vitro studies. Cell 37, 33-41.PubMed ... Mezquita C. (1985) Chromatin Proteins and Chromatin Structure in Spermatogenesis. In: Reeck G.R., Goodwin G.H., Puigdomènech P ...
... chromatin assembly factor I; p300-CBP Transcription Factors, 2.3.1.48; p300-CBP-associated factor, 2.3.1.48 參考文獻: Kaufman, P.D ... chromatin assembly factor I; p300-CBP Transcription Factors, 2.3.1.48; p300-CBP-associated factor, 2.3.1.48 參考文獻: Kaufman, P.D ... It is essential for coupling nucleosome assembly to newly synthesized DNA. Whether chromatin assembly factor 1 subunits have ... It is essential for coupling nucleosome assembly to newly synthesized DNA. Whether chromatin assembly factor 1 subunits have ...
We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 ... Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins ... Interaction between Chromatin Assembly Factor 1 and HP1 Proteins. Molecular Cell, 4 (4). pp. 529-540. ... The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. ...
Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells ... The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been ... Such chromatin recruitment resembles that seen for PCNA, a DNA replication and repair factor. The chromatin-associated fraction ... Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells. Journal of Cell ...
The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not ... Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo ... Hoek, M., Stillman, B. (October 2003) Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA ... De novo chromatin assembly maintains histone density on the daughter strands in the wake of the replication fork. ...
Abbreviations: BER, base excision repair; CAF1-p150, chromatin assembly factor 1 p10 subunit; CHO, Chinese hamster ovary; DIQ, ... anti-chromatin assembly factor 1 p150 subunit (CAF1-p150) (1:50, Merck), and anti-ligase IIIα (LIGIIIα) (1:100, GeneTex, San ... Because PCNA is involved in the late step of the long-patch BER pathway and CAF1 has a role in chromatin assembly in the late ... Because accumulation of PCNA at the irradiated site takes more time than that of POL β, additional factors and steps may be ...
... chromatin assembly factor); (J) mTagBFP2-Rab5a-7; (K) mTagBFP2-α-tubulin-18; (L) mTagBFP2-myosin-IIA-18; (M) mTagBFP2-PCNA-19 ... Subach OM1, Cranfill PJ, Davidson MW, Verkhusha VV.. Author information. 1. Department of Anatomy and Structural Biology, and ... Bands 1 and 2 show the total polypeptide chain and the polypeptide chain after cleavage inside the chromophore, respectively. ( ... D) The ratio of the band 1 intensity to the band 2 intensity for SDS-PAGE analysis presented in . (E) Deconvoluted FT-ICR mass ...
Scopoletin activated NF-κB in a SEAP-driven NF-κB reporter cell line, indicating that NF-κB might be a resistance factor for ... although NF-κB activation may be considered as resistance factor for this compound. Further investigations are warranted to ... which all harbored binding motifs in their promoter sequences for the transcription factor, NF-κB, which is known to be ... Chromatin assembly factor 1, subunit A (p150). Chromatin assembly in DNA replication and DNA repair. ...
Chromatin assembly factor 1 subunit C. gi‖1172846‖. Nuclear. 47,655.74/4.74. 55.2/4.71. 4. 4. 13.18. 1:0.34. ... Chromatin assembly. gi‖1172846‖. Nuclear. 47,655.74/4.74. 1. 1. 3.76. 1:1.63. ... Translation factorb. eIF-1. gi‖1174483‖. No annotation. 8. 2. 1:1.56 ± 0.08. K.FAC*NGTVIEHPEYGEVIQLQGDQR.K (3+/4.16/0.38); K. ... Potassium channel-modulatory factor 1. gi‖31543383‖. No annotation. 1. 1. 1:2.59. R.HEGVSC*DAC*LK.G5 (2+/2.90/0.43). ...
We demonstrate that chromatin in MUG is fully competent to liberate microtubule nucleation and stabilization factors through a ... Relative contributions of chromatin and kinetochores to mitotic spindle assembly. Christopher B. OConnell, Jadranka Lončarek, ... Relative contributions of chromatin and kinetochores to mitotic spindle assembly. Christopher B. OConnell, Jadranka Lončarek, ... Examining how the spatial organization of chromatin signals influences metaphase spindle assembly. Nat. Cell Biol. 8:924-932. ...
Subunit of chromatin assembly factor complex CAF-1 that promotes histone H3 and H4 deposition onto newly synthesized DNA; ... Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at ... chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and ... MSI1 1 Systematic Name. YBR195C. SGD ID. SGD:S000000399. Aliases. CAC3 2 Feature Type. ORF , Verified Description. ...
Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1. ... Host-viral effects of chromatin assembly factor 1 interaction with HCMV IE2. ... Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores. ... Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini. ...
RBBP4 is a subunit of the chromatin assembly factor 1 (CAF-1) complex. The CAF-1 complex is a conserved heterotrimeric protein ... specifically it facilitates replication-dependent nucleosome assembly with the major histone H3 (H3.1). This domain is an alpha ...
Looking for online definition of CAF-1 in the Medical Dictionary? CAF-1 explanation free. What is CAF-1? Meaning of CAF-1 ... A gene on chromosome 19p13.3 that encodes chromatin assembly factor 1, subunit A, a core component of the Chromatin Assembly ... which is thought to mediate chromatin assembly in DNA replication and DNA repair.. ... CAF-1 , definition of CAF-1 by Medical dictionary https://medical-dictionary.thefreedictionary.com/CAF-1 ...
  • Reese BE, Bachman KE, Baylin SB, Rountree MR. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. (ebi.ac.uk)
  • ES cell identity is governed by the specialized homeobox binding protein family (POU) transcription factor Octamer binding protein (Oct-4) and the divergent homeodomain protein pluripotency gene (nanog). (aacrjournals.org)
  • Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells. (ebi.ac.uk)
  • Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. (ebi.ac.uk)
  • Several studies have shown that the interaction between CAF-1 and PCNA, which stabilizes CAF-1 at replication forks, is important for CAF-1's role in nucleosome assembly CAF-1 is required for the spatial organization and epigenetic marking of heterochromatin domains in pluripotent embryonic cells, creating a cellular memory of somatic cell identity during cellular differentiation. (wikipedia.org)
  • Chromatin assembly is mediated by factors that function to deliver the core histones to the sites of DNA synthesis, such as chromatin assembly factor 1 (CAF-1), replication-coupling assembly factor (RCAF), nucleosome assembly protein-1 (NAP-1), and nucleoplasmin, as well as by an ATP-dependent motor protein, such as ATP-utilizing chromatin assembly and remodeling factor (ACF), which catalyzes the assembly of histones into periodic nucleosome arrays. (asm.org)
  • It is essential for coupling nucleosome assembly to newly synthesized DNA. (elsevier.com)
  • We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome assembly during DNA replication. (cshl.edu)
  • We suggest that CAF-1 p150 has a heterochromatin-specific function distinct from its nucleosome assembly function during S phase. (cshl.edu)
  • Consistent with these in vivo observations, we found that the amount of CAF-1 required to stimulate nucleosome assembly during the repair of UV photoproducts in vitro depended upon both the number of lesions and the phosphorylation state of CAF-1. (cshl.edu)
  • specifically it facilitates replication-dependent nucleosome assembly with the major histone H3 (H3.1). (ebi.ac.uk)
  • Drosophila CAF1-55 , a subunit of the Polycomb repressive complex PRC2, is multifunctional and evolutionarily conserved protein that participates in nucleosome assembly and remodelling. (sdbonline.org)
  • The Chd1 motor protein performs two functionally distinct ATP-dependent activities, nucleosome assembly and chromatin remodeling, to generate periodic arrays of nucleosomes. (elifesciences.org)
  • Controlling cell cycle progression in a developing embryo directly depends on de novo histone supply and a specialized surveillance mechanism for nucleosome assembly. (elifesciences.org)
  • Our findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly. (sciencemag.org)
  • This nucleosome assembly pathway involves the histone chaperone chromatin assembly factor 1 (CAF-1). (uzh.ch)
  • SNF2-like protein CHD1 is another ATP-dependent nucleosome assembly factor. (yu.edu)
  • In this review, we examine this process of replication-coupled nucleosome assembly to understand how characteristic steady-state nucleosome landscapes are attained. (sciencemag.org)
  • A fuller understanding of replication-coupled nucleosome assembly will be needed to explain how epigenetic information is replicated at every cell division. (sciencemag.org)
  • Verreault, A. (2000) De Novo Nucleosome Assembly: New Pieces in an Old Puzzle. (scirp.org)
  • Tagami, H., Ray-Gallet, D., Almouzni, G. and Nakatani, Y. (2004) Histone H3.1 and H3.3 Complexes Mediate Nucleosome Assembly Pathways Dependent or Independent of DNA Synthesis. (scirp.org)
  • Lee JS, Zhang Z . O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. (mayo.edu)
  • An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins. (ebi.ac.uk)
  • The basic repeating unit of chromatin, the nucleosome, consists of approximately two turns of DNA wrapped around an octamer of core histone proteins ( 22 ). (asm.org)
  • The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. (cshl.edu)
  • The MS and MS/MS spectra of ICAT analysis for proteins α-tubulin, HSP90, and vimentin are shown in Supplemental Figs. 1-3, respectively. (mcponline.org)
  • The results in this report represent the first evidence that cellular chromatin-remodeling proteins, and SNF2H in particular, can play important roles in regulating the chromatin state of the HSV-1 genome during infection. (asm.org)
  • The largest subunit (p150) of this factor interacts directly with proliferating cell nuclear antigen (PCNA), and critical regions for this interaction on both proteins have been mapped. (uzh.ch)
  • Arabidopsis thaliana contains five genes encoding MSI1-like proteins, but their functions in diverse chromatin-associated complexes are poorly understood. (plantcell.org)
  • Here, we investigated the role of two PRC proteins, StMSI1 (PRC2 member) and StBMI1-1, in potato development. (plantphysiol.org)
  • like histone-fold domain proteins at the crossroad between chromatin and different DNA metabolisms. (xenbase.org)
  • To reconstitute other higher-order chromatin structures, we incorporate modified core histones, histone variants and heterochromatin proteins. (yu.edu)
  • Because only two SIRT7 interactions have been thus far described: RNA Pol I and upstream binding factor, identification of proteins and complexes associating with SIRT7 is critical to understanding its functions. (mcponline.org)
  • Journal Article] ASYMMETRIC-LEAVES2 and an ortholog of eukaryotic NudC domain proteins repress expression of class 1 KNOX homeobox genes for development of flat symmetric leaves in Arabidopsis. (nii.ac.jp)
  • 1 These proteins bind the minor groove of AT-rich DNA sequences through three short basic repeats, named 'AT-hooks', thereby altering chromatin structure and facilitating the assembly of multiprotein complexes of transcriptional factors, thus regulating the transcription of several genes involved in a wide spectrum of biological processes, such as embryogenesis, cell differentiation, cell cycle, cell migration, apoptosis and cell transformation. (nature.com)
  • The second step involves, perhaps, chromatin remodeling, the rearrangement of proteins on the DNA surface to make the DNA more accessible to other transcription factor binding. (hstalks.com)
  • Chromatin-remodeling proteins form crucial complexes that reposition nucleosomes to accomplish this task. (pubmedcentralcanada.ca)
  • Steroid receptors and coactivator proteins are thought to stimulate gene expression by facilitating the assembly of basal transcription factors into a stable preinitiation complex. (nih.gov)
  • Nonlysed cells from cultures treated with TNF do not show any changes in nuclear chromatin, suggesting that neither DNA nor nuclear proteins are the primary targets of the TNF. (aacrjournals.org)
  • Following viral DNA replication, late viral genes, which mainly encode structural proteins, start to transcribe and ultimately lead to the assembly and release of infectious particles. (prolekare.cz)
  • Total cell lysates (15 µg total protein) from HEK293T cells and untreated (-) or treated (+) with UV radiation were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody, clone 11G10SC, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. (biolegend.com)
  • termed p55) subunit of Drosophila melanogaster chromatin assembly factor 1 (dCAF-1), a multisubunit protein that is required for the assembly of nucleosomes onto newly replicated DNA in vitro. (asm.org)
  • Chromatin assembly factor-1 (CAF-1) is a multi protein complex that functions to assemble nucleosomes in a replication-dependent fashion. (proteus-biosciences.com)
  • The CAF1 complex binds single histone H3-H4 dimers, and two such complexes associate with extended DNA elements to ensure the deposition of H3-H4 tetramers, the first step in the assembly of nucleosomes. (elifesciences.org)
  • During productive infection, viral chromatin is generally in a euchromatic state that is conducive to active transcription ( 4 , 5 ), and the nucleosomes associated with HSV-1 DNA are unstable ( 6 ). (asm.org)
  • Incorporation of replacement histones into chromatin occurs throughout the cell cycle, whereas nucleosomes containing canonical histones are assembled exclusively during DNA replication. (sciencemag.org)
  • The molecular motors known to assemble nucleosomes are ACF (ATP-utilizing chromatin assembly and remodeling factor), CHRAC (chromatin accessibility complex), and RSF (nucleosome-remodeling and spacing factor), which contain the Snf2 family member ISWI as the catalytic subunit ( 5 - 7 ), and CHD1, which belongs to the CHD subfamily of Snf2-like adenosine triphosphatases (ATPases) ( 8 ). (sciencemag.org)
  • In the presence of a core histone chaperone NAP-1, ACF/ISWI mediate deposition of histone octamers onto DNA and form arrays of regularly spaced nucleosomes. (yu.edu)
  • We study ACF and ISWI as prototype factors to elucidate elementary molecular events that take place during ATP-dependent formation of nucleosomes. (yu.edu)
  • Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. (abcam.com)
  • Eukaryotic genes are organized into assemblies of core histone-containing nucleosomes. (jimmunol.org)
  • The green balls that you see are the nucleosomes which package the chromatin. (hstalks.com)
  • Between the minus 1 and the plus 1 nucleosomes is an open region where there are no nucleosomes. (hstalks.com)
  • The nucleosome landscape of different cell types is established in large part through processes that disrupt and replace nucleosomes, mainly transcription, chromatin remodeling, and replication. (sciencemag.org)
  • After the new strand of DNA is synthesized by the polymerase, assembly factors reconstitute old nucleosomes and assemble new nucleosomes behind the replication fork. (sciencemag.org)
  • Arabidopsis Chromatin Assembly Factor 1 is required for occupancy and position of a subset of nucleosomes. (semanticscholar.org)
  • To gain a better understanding of the assembly of chromatin during DNA synthesis, we identified, cloned, and characterized the 180- and 105-kDa polypeptides of Drosophila chromatin assembly factor 1 (dCAF-1). (asm.org)
  • The analysis of native and recombinant dCAF-1 revealed an interaction between dCAF-1 and the Drosophila anti-silencing function 1 (dASF1) component of replication-coupling assembly factor (RCAF). (asm.org)
  • Consistent with the interaction between dCAF-1 p105 and dASF1 in vitro, we observed that dASF1 and dCAF-1 p105 colocalized in vivo in Drosophila polytene chromosomes. (asm.org)
  • Chromatin assembly factor 1 (CAF1), a histone chaperone that mediates the deposition of histone H3/H4 onto newly synthesized DNA, is involved in Notch signaling activation during Drosophila wing imaginal disc development. (biologists.org)
  • The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. (asm.org)
  • The composition of CAF-1 is conserved among human Drosophila and yeast. (proteus-biosciences.com)
  • This study identified a conserved domain in the large subunit of the CAF-1 complex required for its interaction with HP1a in the Drosophila fruit fly. (sdbonline.org)
  • Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3.3 into the male pronucleus, renders the paternal genome unable to participate in zygotic mitoses, and leads to the development of haploid embryos. (sciencemag.org)
  • Here, we investigated the role of CHD1 in chromatin assembly in vivo in Drosophila . (sciencemag.org)
  • Here, we report that knockdown of one of the subunits of Drosophila CAF-1, dCAF-1-p105 (Caf1-105), results in phenotypes that resemble those of, and are augmented synergistically by, mutations of Notch positive regulatory pathway components. (biologists.org)
  • Together, our results reveal a novel epigenetic function of dCAF-1 in promoting Notch pathway activity that regulates normal Drosophila development. (biologists.org)
  • In Drosophila , the core components of the Notch pathway consist of ligands Delta/Serrate (Dl/Ser), receptor Notch and the DNA-binding transcription factor Suppressor of Hairless [Su(H)] ( Bray, 2006 ). (biologists.org)
  • ACF is the major ATP-dependent chromatin assembly factor in Drosophila. (yu.edu)
  • Examples of transcription and remodeling establishing the cell type-specific nucleosome landscape include widespread transcription at enhancers ( 1 ), remodeling ( 2 ), and nucleosome turnover ( 3 ) at Polycomb response elements (PREs) in Drosophila , which are sites required for maintaining cell type-specific transcriptional programs through several cell divisions during development. (sciencemag.org)
  • CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. (semanticscholar.org)
  • To gain insights into the in vivo functions of CAF-1 in Drosophila, we generated null mutants of the largest subunit of dCAF-1, dCAF-1-p180. (semanticscholar.org)
  • Drosophila CAF-1 regulates HP1-mediated epigenetic silencing and pericentric heterochromatin stability. (semanticscholar.org)
  • We have found that budding yeast deleted for the genes encoding CAF-1 are highly sensitive to double-strand DNA-damaging agents. (genetics.org)
  • COMPARE and hierarchical cluster analyses of transcriptome-wide mRNA expression resulted in a set of 40 genes, which all harbored binding motifs in their promoter sequences for the transcription factor, NF-κB, which is known to be associated with drug resistance. (mdpi.com)
  • We hypothesize that SNF2H is recruited to viral promoters during HSV-1 infection, where it can remodel the chromatin state of the viral genome, facilitate the transcription of immediate-early genes, and enhance viral replication. (asm.org)
  • However, the cellular chromatin-remodeling factors involved in altering nucleosome occupancy at viral genes remain largely unknown. (asm.org)
  • Two excisions, Df(2L)Chd1[1] and Df(2L)Chd1[2] , deleted fragments of the Chd1 gene and fragments of unrelated adjacent genes. (sciencemag.org)
  • What we found is as follows: 1) This multi-gene family consists of 33 genes in Arabidopsis. (go.jp)
  • Depletion of dCAF-1-p105 leads to abrogation of cut expression and to downregulation of other Notch target genes in wing imaginal discs. (biologists.org)
  • In response to induced Notch activation, dCAF-1 associates with the Notch intracellular domain to activate the expression of Notch target genes in cultured S2 cells, manifesting the role of dCAF-1 in Notch signaling. (biologists.org)
  • The MSI1-HDA19 complex fine-tunes ABA signaling by binding to the chromatin of ABA receptor genes and by maintaining low levels of acetylation of histone H3 at lysine 9, thereby affecting the expression levels of ABA receptor genes. (plantcell.org)
  • RNA-sequencing of axillary nodes from StMSI1 -OE and StBMI1 - 1 - AS lines revealed downregulation of auxin and brassinosteroid genes, and upregulation of cytokinin transport/signaling genes, from 1,023 differentially expressed genes shared between the two lines. (plantphysiol.org)
  • Moreover, we observed downregulation of genes encoding H2A-ubiquitin ligase and StBMI1-1/3, and upregulation of Trithorax group H3K4-methyl-transferases in StMSI1 - OE . (plantphysiol.org)
  • In addition, mutations in genes encoding an essential DNA helicase, DNA2 , or the proliferating cell nuclear antigen (PCNA) loading factor, RFC1 , disrupt silencing at the telomeres and ribosomal DNA (rDNA), respectively ( 15 , 16 ). (sciencemag.org)
  • tobacco plants with antibiotic resistance genes [1]. (deepdyve.com)
  • As prime candidates in the search for roadblock-factors, the team decided to focus on so-called chromatin regulators - genes that control the packaging and bookmarking of DNA, which is known to underlie the "epigenetic memory" of a cell. (innovations-report.com)
  • The results of this screen were striking: Among 615 genes, four factors clearly stood out as top hits. (innovations-report.com)
  • When the researchers thoroughly tested these genes, the effects were stunning: While inhibiting previously described roadblock-genes increases iPSC reprogramming three- to fourfold, losing CAF-1 or UBE2I made this process 50- to 200-fold more efficient. (innovations-report.com)
  • It is accepted that in plants, CDK/cyc complexes phosphorylate the retinoblastoma-related protein (RBR), causing the activation of a set of genes that are regulated by the E2F/DP transcription factor and are necessary for S-phase entry and DNA replication. (plantcell.org)
  • By manipulating those genes and introducing new factors, scientists can unlock dormant parts of an adult cell's genome and reprogram it into another cell type. (innovations-report.com)
  • To identify potential factors, the team established a genetic library targeting known chromatin regulators -- genes that control the packaging and bookmarking of DNA, and are involved in creating cellular memory. (innovations-report.com)
  • These genes have a function related to polarized cell growth, cell cycle regulation or chromatin remodelling. (biomedcentral.com)
  • Acetylation by SRC-1 and PCAF of histones bound at specific promoters may result from ligand binding to steroid receptors and could be a mechanism by which the activation functions of steroid receptors and associated coactivators enhance formation of a stable preinitiation complex, thereby increasing transcription of specific genes from transcriptionally repressed chromatin templates. (nih.gov)
  • The data show a disproportionate influence of YRV on the variable expression of genes whose protein products localize to the nucleus, have nucleic-acid binding activity, and are involved in transcription, chromosome organization, and chromatin assembly. (harvard.edu)
  • Chromatin assembly factor 1 subunit A (CAF1 subunit A or subunit p150) is a core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair [ PMID: 17065558 ]. (ebi.ac.uk)
  • The chromatin assembly factor CAF1 is a conserved histone chaperone, which assembles histones H3 and H4 onto newly synthesized DNA during replication and repair. (sdbonline.org)
  • Altogether, this work demonstrates that chromatin dynamics mediated by CAF1 play an important role in both the regulation of stem cell identity and genome integrity. (sdbonline.org)
  • These data suggest that CAF1 and PCNA link DNA replication to chromatin assembly and silencing. (sciencemag.org)
  • In comparison to the three to four fold increase seen by suppressing previously known roadblock factors, inhibiting the newly described CAF1 (chromatin assembly factor 1) made the process 50 to 200 fold more efficient. (innovations-report.com)
  • Furthermore, we have established that the putative 75-kDa polypeptide of dCAF-1 is a C-terminally truncated form of p105 that does not coexist in dCAF-1 complexes containing the p105 subunit. (asm.org)
  • Mutations in p150 prevent association with HP1 in heterochromatin in cells that are not in S phase and the formation of CAF-1-HP1 complexes in nascent chromatin during DNA replication in vitro. (cshl.edu)
  • Distinct function of 2 chromatin remodeling complexes that share a common subunit, Williams syndrome transcription factor (WSTF). (semanticscholar.org)
  • CDK/cycD complexes act at the G1/S checkpoint by phosphorylating the RBR protein, causing the release of the E2F transcription factor and entry into S-phase. (plantcell.org)
  • The resulting refined proteomic data set confirmed SIRT7 interactions with RNA Pol I and upstream binding factor and highlighted association with factors involved in RNA Pol I- and II-dependent transcriptional processes and several nucleolus-localized chromatin remodeling complexes. (mcponline.org)
  • The down-regulation of RNA Pol I protein levels placed in the context of SIRT7 interaction networks led us to propose that SIRT7 plays a crucial role in connecting the function of chromatin remodeling complexes to RNA Pol I machinery during transcription. (mcponline.org)
  • however, the compositional details of these complexes vary, as evident by comparing yeast and mammalian models ( Fig. 1 ). (pubmedcentralcanada.ca)
  • 2004) Globin gene activation during haemopoiesis is driven by protein complexes nucleated by GATA‐1 and GATA‐2. (els.net)
  • Narlikar GJ, Fan HY and Kingston RE (2002) Cooperation between complexes that regulate chromatin structure and transcription. (els.net)
  • Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. (biolegend.com)
  • Combined, our results support a model in which Cdc7p regulates replication-coupled histone modification via a CAC1 -dependent mechanism involving H4 K16ac deposition, and thereby silencing, while CAF-1-dependent replication- and repair-coupled chromatin assembly per se are functional in the absence of phosphorylation of Cdc7p consensus sites on CAF-1. (genetics.org)
  • In eukaryotic cells, the organization of genomic DNA into chromatin regulates many biological processes, from the control of gene expression to the regulation of chromosome segregation. (sdbonline.org)
  • Like other DNA viruses that replicate in the nucleus, herpes simplex virus 1 (HSV-1) regulates the association of histones with its genome to promote viral replication and gene expression. (asm.org)
  • 2017) The p150N domain of chromatin assembly factor-1 regulates Ki-67 accumulation on the mitotic perichromosomal layer. (umassmed.edu)
  • TNF-α transcriptionally regulates murine monocyte chemoattractant protein-1 (MCP-1) expression. (jimmunol.org)
  • Three approaches were used to determine the mechanism by which TNF regulates MCP-1. (jimmunol.org)
  • Trans -activation studies conducted by cotransfection of p50 and/or p65 expression vectors with MCP-1 constructions showed that TNF regulates MCP-1 through NF-κB. (jimmunol.org)
  • dCAF-1-p105 is associated with Suppressor of Hairless [Su(H)] and regulates its binding to the enhancer region of E(spl)mβ . (biologists.org)
  • However, the molecular mechanisms by which CAF-1 regulates transcription and whether this function is coupled to specific signaling pathways that are essential for animal development remain unclear. (biologists.org)
  • The ultimate goal of our research is to understand how chromosomes are assembled and how chromatin assembly regulates the structure and activity of eukaryotic chromosomes. (yu.edu)
  • For example, we discovered that Chromatin Assembly Factor-1 (CAF-1) regulates protein accumulation and long-range DNA interactions at the nucleolus in human interphase cells. (umassmed.edu)
  • CAF-1 also regulates the protein composition of human mitotic chromosomes. (umassmed.edu)
  • Therefore, CAF-1 appears to be part of an equilibrium that regulates stability/dissociation of early steps of recombination events. (morebooks.de)
  • Among its related pathways are Autophagy Pathway and Chromatin Regulation / Acetylation . (genecards.org)
  • Among its related pathways are Chromatin Regulation / Acetylation and Nanoparticle triggered autophagic cell death . (genecards.org)
  • Chromatin structure is altered following DNA replication stress through the activity of protein kinase C signalling which leads to functionally coupled histone H3 phosphorylation and acetylation events. (elifesciences.org)
  • A versatile acetylation-methylation switch at lysine 31 on the lateral surface of histone H4 contributes to chromatin structure in apicomplexan parasites. (elifesciences.org)
  • The association of dCAF-1-p105 with Su(H) on chromatin establishes an active local chromatin status for transcription by maintaining a high level of histone H4 acetylation. (biologists.org)
  • The correlation between histone acetylation status and transcriptional regulation is well established in that hyperacetylation is commonly associated with transcriptional activation, whereas hypoacetylation is associated with transcriptional repression ( 1 ⇓ - 3 ). (mcponline.org)
  • We studied the roles of the ubiquitous inflammatory transcription factors, NF-κB and AP-1, in activation of the TGF-β1 gene and histone acetylation at the TGF-β1 promoter. (jimmunol.org)
  • Using chromatin immunoprecipitation assays, we observed a sequential recruitment of p65 and c-Jun, accompanying ordered elevation of the levels of histone H4 and H3 acetylation and recruitment of RNA polymerase II at distinct regions in the native TGF-β1 promoter. (jimmunol.org)
  • The available evidence indicates that acetylation of chromatin in vivo is coupled to transcription and that specific histone acetyltransferases (HATs) target histones bound to DNA and overcome the inhibitory effect of chromatin on gene expression. (nih.gov)
  • Forsberg EC and Bresnick EH (2001) Histone acetylation beyond promoters: long‐range acetylation patterns in the chromatin world. (els.net)
  • 2000) Nuclear localization and histone acetylation: a pathway for chromatin opening and transcriptional activation of the human β‐globin locus. (els.net)
  • The double-stranded DNA genome of herpes simplex virus 1 (HSV-1) associates with histones to form chromatin rapidly following infection of a host cell, and the resulting chromatin structure is dynamically regulated throughout productive HSV-1 infection (1-3). (asm.org)
  • Cells resembling 2-cell-stage mouse embryos (totipotent cells) can be induced in vitro through downregulation of the chromatin-assembly activity of CAF-1 in embryonic stem cells. (wikipedia.org)
  • The histone chaperone chromatin assembly factor 1 (CAF-1) mediates histone H3-H4 assembly during DNA replication and nucleotide excision repair in vitro . (genetics.org)
  • This is mediated in part by the histone chaperone chromatin assembly factor 1 (CAF-1), which deposits histones H3 and H4 onto newly replicated DNA in vitro ( S mith and S tillman 1989 ). (genetics.org)
  • CAF-1 was identified as a protein that participates in the assembly of newly synthesized DNA into chromatin during simian virus 40 (SV40) DNA replication in vitro ( 32 , 36 ). (asm.org)
  • The chromatin immunoprecipitation analysis and the in vitro protein-DNA binding assay demonstrated that p150 used its KER domain to associate with the MIEP from -593 to -574 bp. (elsevier.com)
  • Changes in chromatin structure during spermatogenesis in maturing rooster testis as demonstrated by the initiation pattern of ribonucleic acid synthesis in vitro. (springer.com)
  • The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not essential for yeast cell proliferation. (cshl.edu)
  • It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. (thermofisher.com)
  • In vitro, chromatin assembly requires the action of histone chaperones and adenosine triphosphate (ATP)-utilizing factors ( 3 ). (sciencemag.org)
  • We previously demonstrated that CHD1, together with the chaperone NAP-1, assembles nucleosome arrays from DNA and histones in vitro ( 9 ). (sciencemag.org)
  • ACF (ATP-utilizing chromatin assembly factor) was identified on the basis of its ability to mediate ATP-dependent reconstitution of chromatin in vitro. (yu.edu)
  • In vitro assembled chromatin vectors can turn into useful tools in research and therapy. (yu.edu)
  • In principle, the in vitro differentiation of neurosphere-forming cells includes five steps: (1) cessation of proliferation, (2) attachment of the neurosphere, (3) detachment of cells from the neurosphere cell cluster, (4) migration of these cells away from the sphere, and (5) their actual differentiation into different cell types. (jneurosci.org)
  • Our unique and interdisciplinary approaches take advantage of in vitro systems, particularly cytoplasmic extracts prepared from eggs of the frog Xenopus laevis that reconstitute mitotic chromosome condensation and spindle assembly and function in vitro. (berkeley.edu)
  • Smith, S. and Stillman, B. (1991) Stepwise Assembly of Chromatin during DNA Replication in Vitro. (scirp.org)
  • Smith, S. and Stillman, B. (1989) Purification and Characterization of CAF-I, a Human Cell Factor Required for Chromatin Assembly during DNA Replication in Vitro. (scirp.org)
  • CAF-1/p150 knockdown attenuated the anchorage-independent proliferation, migration and invasion of Hela and SiHa cervical cancer cells in vitro. (spandidos-publications.com)
  • Just before mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin concomitant with histone H3 phosphorylation. (cshl.edu)
  • During mitosis and meiosis in animal cells, chromosomes actively participate in spindle assembly by generating a gradient of Ran guanosine triphosphate (RanGTP). (rupress.org)
  • When stable kinetochore microtubule attachment is prevented by Nuf2 depletion in both MUG and normal mitosis, chromatin attracts astral microtubules but cannot induce spindle assembly. (rupress.org)
  • During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. (abcam.com)
  • The gametes without the Gc chromosome are killed due to chromosomal aberrations induced by Gc chromosomes in the G1 phase prior to DNA synthesis of the first post-meiotic mitosis, including chromatid fragments, dicentric chromosomes, and chromatin bridges (Finch et al. (deepdyve.com)
  • The main functions of chromatin include packaging of DNA into a compact shape, reinforcement of the DNA molecules in such a way to allow the process of mitosis and controlling gene expression and DNA replication. (intechopen.com)
  • The present data implicate metabolic changes which occur during mitosis (cytokinesis), perhaps associated with synthesis or assembly of cell membrane components, as being responsible for increased cell sensitivity towards the cytolytic effect of TNF. (aacrjournals.org)
  • The eukaryotic nuclear genome is assembled into the nucleoprotein structure termed chromatin. (genetics.org)
  • Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field. (springer.com)
  • The assembly of DNA into chromatin and dynamic conversion between its different forms are critical steps in the maintenance and regulation of the eukaryotic genome. (yu.edu)
  • Furthermore, chromatin immunoprecipitation (ChIP) studies showed that SNF2H associated with the HSV-1 genome during infection, which suggests that SNF2H may directly remodel viral chromatin. (asm.org)
  • Chromatin immunoprecipitation-quantitative PCR confirmed H3K27me3-mediated suppression of StBMI1 - 1 / 3 , and H3K4me3-mediated activation of miR156 in StMSI1 - OE plants. (plantphysiol.org)
  • Second, we showed that regulatory regions of WUS were developmentally regulated by both DNA methylation and histone modifications through bisulfite sequencing and chromatin immunoprecipitation. (prolekare.cz)
  • The replication dependence of chromatin assembly by CAF-1 appears to be due to the targeting of CAF-1 to DNA replication forks via its interaction with the proliferating cell nuclear antigen (PCNA) ( S hibahara and S tillman 1999 ). (genetics.org)
  • The structure and dynamics of chromatin have far-ranging consequences for many nuclear processes, such as DNA replication, transcription, recombination, and repair ( 44 ). (asm.org)
  • Changes in nuclear and chromatin composition and genomic activity during spermatogenesis in the maturing rooster testis. (springer.com)
  • Nuclear DNA viruses generally encode gene products that function to counteract this inhibitory cellular response by coordinating the modification of these histones and/or by remodeling the viral chromatin state through the redirection of cellular enzymes to the viral genome to allow for efficient viral replication and gene expression. (asm.org)
  • The organization of chromatin affects all aspects of nuclear DNA metabolism in eukaryotes. (sciencemag.org)
  • High mobility group 1 (HMGB1) protein is both a nuclear factor and a secreted protein. (nih.gov)
  • Chromatin is the natural state of DNA in the nucleus and the native substrate for nuclear reactions such as DNA replication, recombination, repair and transcription. (yu.edu)
  • The association between intracellular danger signaling involving nuclear chromatin-binding factor, high mobility group box-1 (HMGB1), and inflammatory pathways following TBI has not yet been fully understood. (pubmedcentralcanada.ca)
  • Intracellular HMGB1 is a nuclear chromatin-binding factor that promotes protein assembly and binds DNA to facilitate gene transcription. (pubmedcentralcanada.ca)
  • Macromolecular cellular assemblies like the ribosome or nuclear pore self assemble from a fixed number of subunits and do not vary in size. (berkeley.edu)
  • Wade, P.A. and Kikyo, N. (2002) Chromatin Remodeling in Nuclear Cloning. (scirp.org)
  • We investigated its cellular localization and found it in both cytosolic and nuclear extracts derived from HeLa cells (Fig. 1 a). (biomedcentral.com)
  • A gene on chromosome 21q22.13 that encodes chromatin assembly factor 1, subunit B, a core component of the Chromatin Assembly Factor I (CAF-1) complex, which is thought to mediate chromatin assembly in DNA replication and DNA repair. (thefreedictionary.com)
  • These results support a model in which kinetochores play the dominant role in the chromosome-mediated pathway of mitotic spindle assembly. (rupress.org)
  • A gene on chromosome 19p13.3 that encodes chromatin assembly factor 1, subunit A, a core component of the Chromatin Assembly Factor I (CAF-1) complex, which is thought to mediate chromatin assembly in DNA replication and DNA repair. (thefreedictionary.com)
  • Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1. (go.jp)
  • Chromatin, the central nucleoprotein filament of a chromosome, has many different forms and organization levels. (yu.edu)
  • Thus, we are trying to uncover the intricate network of assembly factors and their roles in the hierarchical organization of the chromosome. (yu.edu)
  • Furthermore, studies have reported that DNA methylation can repress chromosome breakages induced by the Gc factors (de Las Heras et al. (deepdyve.com)
  • Chromosome 19 has been identified as having the highest gene density within the human chromosomes, with large gene families of evolutionary significance [ 1 ]. (hindawi.com)
  • Taken together, our results indicate that Y chromosome heterochromatin serves as a major source of epigenetic variation in natural populations that interacts with chromatin components to modulate the expression of biologically relevant phenotypic variation. (harvard.edu)
  • In Arabidopsis thaliana , CAF-1 is important for the stable maintenance of gene expression states at shoot and root apical meristems ( 19 ). (asm.org)
  • IMPORTANCE It is becoming increasingly appreciated that regulation of the state of chromatin is a major determinant in control of gene expression. (asm.org)
  • This work also further establishes HSV-1 infection as a useful model to study chromatin control of gene expression and suggests that disrupting the regulation of viral chromatin states can possibly be exploited as a novel antiviral therapeutic target. (asm.org)
  • Several viral gene products have been implicated in playing a role in generation of the euchromatin-like state of the viral genome ( 1 , 2 , 7 , 8 ), suggesting that HSV-1 encodes multiple activities to ensure that its genome remains competent for transcription despite the cellular response that attempts to silence foreign gene expression. (asm.org)
  • it remains unclear whether CAF-1 has a role in specific signaling cascades to promote gene expression during development. (biologists.org)
  • DNA replication can play a role in changing patterns of gene expression ( 1-3 ) and thus is a possible mechanism for disrupting chromatin states before their reprogramming and for the de novo establishment of those states. (sciencemag.org)
  • To regulate gene expression, transcription factors and RNA polymerases must be able to search packed chromatin efficiently, then locate and interact with their target DNA binding sites. (jimmunol.org)
  • Chromatin-remodeling enzymes play essential roles in many biological processes, including gene expression, DNA replication and repair, and cell division. (pubmedcentralcanada.ca)
  • These phenotypes in Saccharomyces cerevisiae included the ability to undergo mating-type switching ( 1 ), increased mutation rates of mtDNA ( 2 ), and regulation of invertase gene expression in sugar metabolism ( 3 ). (pubmedcentralcanada.ca)
  • Recently, we and others have identified several viral factors that regulate gene expression during late infection. (prolekare.cz)
  • Here we show that HCMV pUL79, one of these factors, interacts with RNAP II as well as other viral factors involved in late gene expression. (prolekare.cz)
  • Chromatin assembly factor-1 (CAF-1) is a protein complex - including Chaf1a (p150), Chaf1b (p60), and p50 subunits - that assembles histone tetramers onto replicating DNA. (wikipedia.org)
  • Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. (rcsb.org)
  • Subunit of the CAF-1 complex that contains RBBP4 , CHAF1B and CHAF1A . (rcsb.org)
  • The purified recombinant p180+p105+p55 dCAF-1 complex is active for DNA replication-coupled chromatin assembly. (asm.org)
  • CAF-1 binds to histones H3 and H4 ( 15 , 33 ), and the protein can be isolated as a complex with histones H3 and (acetylated) H4 ( 42 ). (asm.org)
  • The CAF-1 complex is composed of three subunits, p150, p60 and p48 in stoichiometric amounts. (proteus-biosciences.com)
  • Localization of the CAF-1 complex to the newly synthesized DNA is mediated in part by PCNA which functions to "mark" the appropriate places for loading of CAF-1 and in fact, PCNA may be co-precipitated with CAF-1. (proteus-biosciences.com)
  • Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. (genecards.org)
  • Whether chromatin assembly factor 1 subunits have functions beyond escorting histones, which depends on the complex formation of p150 and p60, has been an issue of great interest. (elsevier.com)
  • This mechanism is thought to rely on the activity of RanGTP, which induces liberation of microtubule nucleation factors sequestered in the complex with importins. (rupress.org)
  • RBBP4 is a subunit of the chromatin assembly factor 1 (CAF-1) complex. (ebi.ac.uk)
  • The Polycomb group protein SCML2 contributes to the assembly and gene silencing function of Polycomb Repressive Complex 1 (PRC1) and requires an RNA-binding region to reach chromatin targets. (elifesciences.org)
  • For example, H3.3 associates with a complex containing HIRA, whereas canonical H3 is in a complex with CAF-1 (chromatin assembly factor 1) ( 4 ). (sciencemag.org)
  • Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. (abcam.com)
  • The CAF-1 complex preserves the identity of cells. (innovations-report.com)
  • The new hits include CHAF1A and CHAF1B, forming the CAF-1-complex (chromatin assembly factor 1), and UBE2I (ubiquitin-conjugating enzyme E2I). (innovations-report.com)
  • The CAF-1 complex ensures that during DNA replication and cell division daughter cells keep their epigenetic memory, which is encoded on the histones that the DNA is wrapped around," explains Ulrich Elling. (innovations-report.com)
  • In finding CAF-1, the researchers identified a complex that allows cell memory to be erased and rewritten, which is a long-awaited breakthrough for stem cell research. (innovations-report.com)
  • actin related protein 2/3 complex, subunit 1. (broadinstitute.org)
  • Truncated Cdc6 interferes with loading of Mcm2 on the chromatin and thus disrupts assembly of the prereplication complex on chromosomes. (rupress.org)
  • Oxidative base damage and single-strand breaks (SSBs) are the most frequent types of DNA damage caused by reactive oxygen species, and such DNA damage can cause transcription and replication block, leading to cell death and genomic instability ( 1 , 2 ). (pnas.org)
  • In vivo genomic footprinting (IVGF) of the upstream flanking DNA of the MCP-1 gene in both resting and TNF-treated BALB/3T3 murine fibroblasts ( 4 ) showed that TNF induced multiple DMS-resistant and DMS-hypersensitive bases in two regions: a distal regulatory region and a proximal regulatory region. (jimmunol.org)
  • Our research is highly collaborative and will provide new insight into the underlying principles of spindle assembly and biological size control, as well as the molecular basis of variation that contributes to genomic instability and evolution. (berkeley.edu)
  • The aim of this project is to combine powerful genetic, biochemical, genomic and systems biology approaches available in the model bacterium Bacillus subtilis with modern high-resolution light microscopic techniques to study the dynamics and mechanistic details of the MreB cytoskeleton and of CW assembly. (europa.eu)
  • To test this prediction, genomic bisulphite sequencing was carried out on 473 bp of the H19 imprinting control region in DNA obtained from midgestation F(1) hybrid mouse embryos (C57BL/6 Ã Mus musculus castaneus ) exposed to ethanol during preimplantation development. (las.ac.cn)
  • Supporting a role in assembling chromatin following DNA replication in vivo , CAF-1 has been shown to localize to sites of DNA replication in tissue culture cells ( K rude 1995 ). (genetics.org)
  • CAF-1-mediated resistance to DNA damage is dependent on the ability of CAF-1 to bind PCNA, indicating that PCNA may recruit CAF-1 to sites of double-strand DNA repair. (genetics.org)
  • CAF-1 is an evolutionarily conserved H3/H4 histone chaperone that plays a key role in replication-coupled chromatin assembly and is targeted to the replication fork via interactions with PCNA , which, if disrupted, leads to epigenetic defects. (genetics.org)
  • CAF-1 has been found to be localized to sites of DNA replication ( 20 , 23 , 37 ), and a specific interaction has been observed between CAF-1 and the PCNA component of the DNA replication and repair machinery ( 26 , 30 , 45 ). (asm.org)
  • Such chromatin recruitment resembles that seen for PCNA, a DNA replication and repair factor. (cshl.edu)
  • A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage. (uzh.ch)
  • The binding of both PCNA and CAF-1 to this damaged DNA was dependent on the number of DNA lesions and required ATP. (uzh.ch)
  • Chromatin assembly linked to the repair of single-strand breaks was disrupted by depletion of PCNA from a cell-free system. (uzh.ch)
  • This defect was rescued by complementation with recombinant PCNA, arguing for role of PCNA in mediating chromatin assembly linked to DNA repair. (uzh.ch)
  • We discuss the importance of the PCNA-CAF-1 interaction in the context of DNA damage processing and checkpoint control. (uzh.ch)
  • Finally, mutant forms of PCNA defective in CAF-1 interaction are defective in establishing silencing ( 19 , 20 ). (sciencemag.org)
  • Here we provide evidence that the Williams syndrome transcription factor (WSTF) is targeted to replication foci through direct interaction with the DNA clamp PCNA, an important coordinator of DNA and chromatin replication. (semanticscholar.org)
  • Zhang K, Gao Y, Li J, Burgess R, Han J, Liang H, Zhang Z , Liu Y. A DNA binding winged helix domain in CAF-1 functions with PCNA to stabilize CAF-1 at replication forks. (mayo.edu)
  • Thus, microtubule nucleation and organization near the chromosomes appears to be a principal driver of spindle assembly even in centrosomal cells. (rupress.org)
  • The replication of chromosomes can be challenged by endogenous and environmental factors,interfering with the progression of replication forks. (morebooks.de)
  • 2. How do biochemical signals from chromosomes contribute to spindle assembly? (berkeley.edu)
  • We demonstrate Cdc7p and Cac1p interact in vivo in S phase, but not in G1, consistent with observed cell cycle-dependent phosphorylation of Cac1p , and hypoacetylation of chromatin at H4 K16 in both cdc7 and cac1 Δ mutants. (genetics.org)
  • These factors have not been shown to mediate deposition of histones in vivo. (sciencemag.org)
  • Examination of MCP-1 induction in NF-κB-disrupted embryonic fibroblasts showed that p65 was necessary for both the induction and the TNF-induced protein occupancy of the enhancer in vivo. (jimmunol.org)
  • This study illustrates the crucial role for NF-κB p65 in the induction of the MCP-1 gene by TNF and in the assembly of a NF-κB dependent enhancer in vivo. (jimmunol.org)
  • In vivo occupancy in the proximal regulatory region identified an SP-1 binding site and two novel sites (κB-3 and site B). The distal regulatory region, which contains two κB binding sites, a novel site (A) and a DMS-hypersensitive (HS) sequence located between the κB sites, was required for TNF-activated expression of wild-type MCP-1 reporter constructions ( 4 ). (jimmunol.org)
  • We disrupted Chd1 in flies and discovered that CHD1 is specifically required for replication-independent deposition of histones into chromatin in vivo. (yu.edu)
  • What is not clear, however, is how these transcription factors gain access to transcriptionally repressed chromatin to modulate the transactivation of specific gene networks in vivo. (nih.gov)
  • IL-1β-induced TGF-β1 protein secretion and mRNA expression were prevented by actinomycin D and were attenuated by the inhibitor of κB kinase 2 inhibitor AS602868 and the JNK inhibitor SP600125, suggesting a degree of transcriptional regulation mediated by the NF-κB and AP-1 pathways. (jimmunol.org)
  • Using reporter gene assays, the transcription factors AP-1 ( 16 ) and SV40 early promoter 1 ( 17 ) have previously been implicated in the transcriptional induction of TGF-β1. (jimmunol.org)
  • Today's talk is on the Genome-wide Organization of Chromatin and the Transcriptional Machinery. (hstalks.com)
  • The third step is the assembly of the general transcriptional machinery in the initiation phase. (hstalks.com)
  • These factors are functionally conserved among beta- and gamma- herpesviruses, suggesting a unique transcriptional regulation shared by viruses of these two subfamilies. (prolekare.cz)
  • A thorough understanding of the replication-independent mechanisms of chromatin assembly, however, is lacking. (sciencemag.org)
  • To this end we use biochemical approaches to analyze mechanisms of chromatin assembly by histone chaperones and ATP-dependent enzymes. (yu.edu)
  • Cell cycle regulation of chromatin at an origin of DNA replication. (semanticscholar.org)
  • FSM/FAS1 and GTE4 are known in rice and Arabidopsis to be involved in the maintenance of root meristem through chromatin remodelling and cell cycle regulation respectively. (biomedcentral.com)
  • The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been investigated after UV irradiation of HeLa cells. (cshl.edu)
  • Chromatin assembly factor 1 subunit B is a protein that in humans is encoded by the CHAF1B gene. (wikipedia.org)
  • Our genetic analyses indicate that CAF-1 plays a role in both homologous recombination and nonhomologous end-joining pathways and that the function of CAF-1 during double-strand repair is distinct from that of another histone H3-H4 chaperone, anti-silencing function 1 ( ASF1 ). (genetics.org)
  • During MUG, chromatin is rapidly separated from the forming spindle, and both centrosomal and noncentrosomal spindle assembly pathways are active. (rupress.org)
  • 2000) Ordered recruitment of chromatin modifying and general transcription factors to the IFN‐beta promoter. (els.net)
  • Li B, Carey M and Workman JL (2007) The role of chromatin during transcription. (els.net)
  • CROWN ROOTLESS1 ( CRL1 ) is induced by auxin and encodes an AS2/LOB-domain transcription factor that acts upstream of the gene regulatory network controlling CR development. (biomedcentral.com)
  • general transcription factor IIH, polypeptid. (broadinstitute.org)
  • Phytohormones and transcription factor WUSCHEL (WUS) play critical roles in this process but whether and how epigenetic modifications are involved is unknown. (prolekare.cz)
  • however, the repeating subunits of chromatin are highly dynamic and flexible in nature [ 1 ]. (intechopen.com)
  • CAF-1 has also been implicated in single-strand DNA repair via the nucleotide excision repair (NER) pathway. (genetics.org)
  • We suggest that berberine and camptothecin inhibit factors that might be required for leaf adaxial cell differentiation through a pathway independent of AS1 and AS2. (nii.ac.jp)
  • HMGA1-knockdown increases autophagosome formation by constraining the activity of the mTOR pathway, a major regulator of autophagy, and transcriptionally upregulating the autophagy-initiating kinase Unc-51-like kinase 1 (ULK1). (nature.com)
  • This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). (cellsignal.com)
  • These results indicate that, in contrast to yeast, human CAF-1 is necessary for coupling chromatin assembly with DNA replication. (cshl.edu)
  • Winkler DD, Zhou H, Dar MA, Zhang Z , Luger K. Yeast CAF-1 assembles histone (H3-H4) 2 tetramers prior to DNA deposition. (mayo.edu)
  • We show that, unlike CAF-1 mutant yeast, dCAF-1-p180 mutant flies are hemizygous lethal. (semanticscholar.org)
  • This interaction between dCAF-1 and dASF1 may be a key component of the functional synergy observed between RCAF and dCAF-1 during the assembly of newly synthesized DNA into chromatin. (asm.org)
  • Host-viral effects of chromatin assembly factor 1 interaction with HCMV IE2. (nih.gov)
  • The Chromatin Assembly Factor 1 (CAF-1) is involved in the assembly of H3-H4 histone dimers on newly synthesized DNA and in the maintenance of a higher order structure, the heterochromatin, through an interaction of its large subunit with the heterochromatin protein HP1a . (sdbonline.org)
  • Importantly, the identification in the large subunit of CAF-1 of a domain required for its interaction with HP1 allows the separation of its functions in heterochromatin related processes from its function in the assembly of H3-H4 dimers onto newly synthesized DNA. (sdbonline.org)
  • Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1. (semanticscholar.org)
  • Here we show that SRC-1 possesses intrinsic histone acetyltransferase activity and that it also interacts with another HAT, p300/CBP-associated factor (PCAF). (nih.gov)
  • We previously demonstrated that SNF2H, a member of the ISWI family of chromatin-remodeling factors, is concentrated in HSV-1 replication compartments in the nuclei of infected cells, suggesting that this cellular enzyme plays a role in viral replication. (asm.org)
  • In the nucleus, DNA is packaged into a nucleoprotein structure known as chromatin. (asm.org)
  • Together, these experiments suggest that NF-κB is involved in MCP-1 induction, but the exact composition and mechanism of promoter occupancy by NF-κB have not been determined. (jimmunol.org)
  • However, the precise factors forming this roadblock and their mechanism of action so far are poorly understood. (innovations-report.com)
  • We wanted to find out which chromatin factors stabilize this memory and which mechanism prevents iPS cells from forming. (innovations-report.com)
  • The mechanism of the early cytostatic effect of the factor is unknown. (aacrjournals.org)
  • 2007) A two‐step, PU.1 dependent, mechanism for developmentally regulated chromatin remodelling and transcription of the c‐fms gene. (els.net)
  • Partial correlations suggested that the relationship between ethanol and placental weight partly depended on DNA methylation at a CCCTC-binding factor site on the paternal allele in placentae, suggesting a novel mechanism of ethanol-induced growth retardation. (las.ac.cn)
  • Therefore, our study discovers a previously uncharacterized mechanism where RNAP II activity is modulated by viral factor pUL79, and potentially other viral factors as well, for coordinated viral transcription. (prolekare.cz)
  • Here, we observed that loss of Cac1p , the largest subunit of CAF-1, also restores silencing at HMR a e** , and silencing in both cac1 Δ and cdc7 mutants is suppressed by overexpression of SAS2 . (genetics.org)
  • After UV irradiation, the chromatin-associated form of CAF-1 dramatically increased in all cells irrespective of their position in the cell cycle. (cshl.edu)
  • The compaction of chromatin can vary depending on the type of cell and the phase of cell cycle that the cell is in. (intechopen.com)
  • TNF exerts little effect on rates of cell progression through G 1 or S phases of the cell cycle, although few cells die during S phase. (aacrjournals.org)
  • Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. (biomedcentral.com)
  • The major histone modifications classically associated with repressed chromatin domains include H3K9 trimethylation (H3K9me3), which is found in constitutive heterochromatin, and H3K27 trimethylation (H3K27me3), which is found in facultative heterochromatin. (sciencemag.org)
  • This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. (springer.com)
  • Histones, the structural unit of chromatin, must be assembled/dissembled to preserve or change chromatin organization in accordance to cellular needs. (scirp.org)
  • 1/2000 - 1/10000. (abcam.com)
  • Orphanides, G. and Reinberg, D. (2000) RNA Polymerase II Elongation through Chromatin. (scirp.org)
  • Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. (antibodies-online.com)
  • Recent studies have highlighted the importance of histone variants, such as H3.3, CENP-A (centromere protein A), or H2A.Z, in chromatin dynamics ( 1 , 2 ). (sciencemag.org)
  • A high concentration of RanGTP promotes microtubule nucleation and stabilization in the vicinity of chromatin. (rupress.org)
  • The Ran guanine nucleotide exchange factor (RanGEF) RCC1 converts RanGDP to RanGTP in the vicinity of chromatin, whereas Ran GTPase-activating protein (RanGAP) promotes GTP hydrolysis in the cytoplasm. (rupress.org)
  • We show here that small interfering RNA (siRNA)-mediated knockdown of SNF2H in HEp-2 cells resulted in an approximately 20-fold decrease in HSV-1 replication, arguing that SNF2H promotes efficient HSV-1 replication. (asm.org)
  • Histone H3 occupancy on viral promoters was increased in HSV-1-infected cells that were transfected with SNF2H-specific siRNAs, suggesting that SNF2H promotes removal of histones from viral promoters during infection. (asm.org)
  • In the cell nucleus it acts as an architectural chromatin-binding factor that bends DNA and promotes protein assembly on specific DNA targets. (nih.gov)
  • Yang S, Long Q, Chen M, Liu X and Zhou H: CAF‑1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer. (spandidos-publications.com)
  • Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. (cellsignal.com)
  • CAF-1 does not protect the genome by assembling it into a damage-resistant chromatin structure, because induction of CAF-1 after DNA damage is sufficient to restore viability. (genetics.org)
  • It has also become clear that the state of chromatin of the herpes simplex virus 1 (HSV-1) genome is dynamically regulated during both productive and latent stages of infection. (asm.org)
  • Additionally, the association of histones with the HSV-1 genome is decreased at late times postinfection ( 1 , 3 ). (asm.org)
  • One viral gene product that has been shown to play a role in regulating the chromatin state of the viral genome is VP16. (asm.org)
  • The dynamic structure of chromatin not only aids in wrapping the entire colossal genome into the boundaries of the nucleus but also plays an imperative role in regulating the accessibility of the DNA for various processes and mechanisms like recombination, transcription, replication, and repair. (intechopen.com)
  • And then, that's followed in step four by the recruitment of RNA polymerase in elongation factors that ultimately need to enter into the gene and transcribe the genome. (hstalks.com)
  • The first is cell division, in particular the assembly and function of the mitotic spindle, which in all eukaryotes performs the universal and essential task of accurately distributing the duplicated genome to two daughter cells. (berkeley.edu)
  • This entry represents the C-terminal domain of CAF-1 subunit p150, which is part of the binding region to the p60 subunit [ PMID: 12697822 , PMID: 21209461 ]. (ebi.ac.uk)
  • My data support a model according to which CAF-1 allows the stabilization of early recombination intermediates, via nucleosome deposition during the elongation of these intermediates. (morebooks.de)
  • In rod-shaped bacteria, MreB homologues assemble into dynamic structures thought to control shape by serving as organizers for the movement and assembly of macromolecular machineries that effect sidewall elongation. (europa.eu)
  • To fully understand these repair processes, we have to focus on chromatin, the template upon which repair occurs. (genetics.org)
  • Histone chaperones play a crucial role during chromatin assembly, thus I decided to focus on the H3-H4 histone chaperone Chromatin Assembly Factor 1 (CAF-1), to study its role in HR processes in S. pombe. (morebooks.de)
  • Chromatin remodeling, ubiquitylation, and DNA damage repair may be regarded as three discrete processes, but in fact, they are three extremely important interlinked processes that are imperative for the sustenance for life. (intechopen.com)
  • TIF1β is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. (biolegend.com)
  • In an asynchronous cell population only a subfraction of the two large CAF-1 subunits, p150 and p60, were found to exist in a chromatin-associated fraction. (cshl.edu)