Chromatin Assembly Factor-1: A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA. It is comprised of three different subunits of 48, 60, and 150 kDa molecular size. The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling.Chromatin: The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.Chromatin Assembly and Disassembly: The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.Chromosomal Proteins, Non-Histone: Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.Retinoblastoma-Binding Protein 4: A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Nucleosomes: The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.Molecular Chaperones: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.Retinoblastoma-Binding Protein 7: A retinoblastoma-binding protein that has an affinity for core HISTONES. It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA Replication: The process by which a DNA molecule is duplicated.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Proliferating Cell Nuclear Antigen: Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.Heterochromatin: The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Nucleosome Assembly Protein 1: A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA.Cell Cycle Proteins: Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Histone Acetyltransferases: Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Histone Chaperones: Proteins involved in the assembly and disassembly of HISTONES into NUCLEOSOMES.S Phase: Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Gene Silencing: Interruption or suppression of the expression of a gene at transcriptional or translational levels.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Fungal Proteins: Proteins found in any species of fungus.Acetylation: Formation of an acetyl derivative. (Stedman, 25th ed)Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome: A subunit of the anaphase-promoting complex whose primary function is to provide structural support for the catalytic and substrate-recognition modules of the complex. Apc5, along with Apc4, tethers the tetratricopeptide-coactivator binding subcomplex to the main structural subunit, Apc1.Nucleoplasmins: A family of histone molecular chaperones that play roles in sperm CHROMATIN decondensation and CHROMATIN ASSEMBLY in fertilized eggs. They were originally discovered in XENOPUS egg extracts as histone-binding factors that mediate nucleosome formation in vitro.Chromatin Immunoprecipitation: A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.DNA, Superhelical: Circular duplex DNA isolated from viruses, bacteria and mitochondria in supercoiled or supertwisted form. This superhelical DNA is endowed with free energy. During transcription, the magnitude of RNA initiation is proportional to the DNA superhelicity.Micrococcal Nuclease: An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.Ribosome Subunits, Large, Eukaryotic: The large subunit of the 80s ribosome of eukaryotes. It is composed of the 28S RIBOSOMAL RNA, the 5.8S RIBOSOMAL RNA, the 5S RIBOSOMAL RNA, and about 50 different RIBOSOMAL PROTEINS.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Centromere: The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Protein Subunits: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Virus Assembly: The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.Cell Extracts: Preparations of cell constituents or subcellular materials, isolates, or substances.Acetyltransferases: Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.Mitosis: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Two-Hybrid System Techniques: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.Xenopus laevis: The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Electron Transport Complex I: A flavoprotein and iron sulfur-containing oxidoreductase complex that catalyzes the conversion of UBIQUINONE to ubiquinol. In MITOCHONDRIA the complex also couples its reaction to the transport of PROTONS across the internal mitochondrial membrane. The NADH DEHYDROGENASE component of the complex can be isolated and is listed as EC 1.6.99.3.Xenopus: An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.Lysine: An essential amino acid. It is often added to animal feed.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Yeasts: A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Histone Deacetylases: Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.Mitochondrial Diseases: Diseases caused by abnormal function of the MITOCHONDRIA. They may be caused by mutations, acquired or inherited, in mitochondrial DNA or in nuclear genes that code for mitochondrial components. They may also be the result of acquired mitochondria dysfunction due to adverse effects of drugs, infections, or other environmental causes.Epigenesis, Genetic: A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Deoxyribonuclease I: An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Electron Transport Complex IV: A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.Methanococcales: An order of anaerobic methanogens in the kingdom EURYARCHAEOTA. They are pseudosarcina, coccoid or sheathed rod-shaped and catabolize methyl groups. The cell wall is composed of protein. The order includes one family, METHANOCOCCACEAE. (From Bergey's Manual of Systemic Bacteriology, 1989)

Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. (1/113)

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.  (+info)

WD repeats of the p48 subunit of chicken chromatin assembly factor-1 required for in vitro interaction with chicken histone deacetylase-2. (2/113)

Chromatin assembly factor-1 (CAF-1) is essential for chromatin assembly in eukaryotes, and comprises three subunits of 150 kDa (p150), 60 kDa (p60), and 48 kDa (p48). We cloned and sequenced cDNA encoding the small subunit of the chicken CAF-1, chCAF-1p48. It consists of 425 amino acid residues including a putative initiation Met, possesses seven WD repeat motifs, and contains only one amino acid change relative to the human and mouse CAF-1p48s. The immunoprecipitation experiment followed by Western blotting revealed that chCAF-1p48 interacts with chicken histone deacetylases (chHDAC-1 and -2) in vivo. The glutathione S-transferase pulldown affinity assay revealed the in vitro interaction of chCAF-1p48 with chHDAC-1, -2, and -3. We showed that the p48 subunit tightly binds to two regions of chHDAC-2, located between amino acid residues 82-180 and 245-314, respectively. We also established that two N-terminal, two C-terminal, or one N-terminal and one C-terminal WD repeat motif of chCAF-1p48 are required for this interaction, using deletion mutants of the respective regions. These results suggest that chCAF-1p48 is involved in many aspects of DNA-utilizing processes, through alterations in the chromatin structure based on both the acetylation and deacetylation of core histones.  (+info)

Chromatin replication: Finding the right connection. (3/113)

Nucleosomes are preferentially assembled on replicating DNA by chromatin assembly factor 1; recent studies have shown that replicated DNA is marked for assembly into chromatin by the replication-fork-associated protein PCNA.  (+info)

Chromatin assembly during DNA replication in somatic cells. (4/113)

Newly replicated DNA is assembled into chromatin through two principle pathways. Firstly, parental nucleosomes segregate to replicated DNA, and are transferred directly to one of the two daughter strands during replication fork passage. Secondly, chromatin assembly factors mediate de-novo assembly of nucleosomes on replicating DNA using newly synthesized and acetylated histone proteins. In somatic cells, chromatin assembly factor 1 (CAF-1) appears to be a key player in assembling new nucleosomes during DNA replication. It provides a molecular connection between newly synthesized histones and components of the DNA replication machinery during the S phase of the cell division cycle.  (+info)

Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins. (5/113)

Mechanisms contributing to the maintenance of heterochromatin in proliferating cells are poorly understood. We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome assembly during DNA replication. Mutations in p150 prevent association with HP1 in heterochromatin in cells that are not in S phase and the formation of CAF-1-HP1 complexes in nascent chromatin during DNA replication in vitro. We suggest that CAF-1 p150 has a heterochromatin-specific function distinct from its nucleosome assembly function during S phase. Just before mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin concomitant with histone H3 phosphorylation. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.  (+info)

Duplication and maintenance of heterochromatin domains. (6/113)

To investigate the mechanisms that assure the maintenance of heterochromatin regions, we took advantage of the fact that clusters of heterochromatin DNA replicate late in S phase and are processed in discrete foci with a characteristic nuclear distribution. At the light microscopy level, within these entities, we followed DNA synthesis, histone H4 acetylation, heterochromatin protein 1 (Hp1alpha and -beta), and chromatin assembly factor 1 (CAF-1). During replication, Hp1alpha and -beta domains of concentration are stably maintained, whereas heterochromatin regions are enriched in both CAF-1 and replication-specific acetylated isoforms of histone H4 (H4Ac 5 and 12). We defined a time window of 20 min for the maintenance of this state. Furthermore, treatment with Trichostatin A (TSA), during and after replication, sustains the H4Ac 5 and 12 state in heterochromatin excluding H4Ac 8 and 16. In comparison, early replication foci, at the same level, did not display any specific enrichment in H4Ac 5 and 12. These data emphasize the specific importance for heterochromatin of the replication-associated H4 isoforms. We propose that perpetuation of heterochromatin involves self-maintenance factors, including local concentration of Hp1alpha and -beta, and that a degree of plasticity is provided by the cycle of H4 acetylation/deacetylation assisted by CAF-1.  (+info)

A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage. (7/113)

Sensing DNA damage is crucial for the maintenance of genomic integrity and cell cycle progression. The participation of chromatin in these events is becoming of increasing interest. We show that the presence of single-strand breaks and gaps, formed either directly or during DNA damage processing, can trigger the propagation of nucleosomal arrays. This nucleosome assembly pathway involves the histone chaperone chromatin assembly factor 1 (CAF-1). The largest subunit (p150) of this factor interacts directly with proliferating cell nuclear antigen (PCNA), and critical regions for this interaction on both proteins have been mapped. To isolate proteins specifically recruited during DNA repair, damaged DNA linked to magnetic beads was used. The binding of both PCNA and CAF-1 to this damaged DNA was dependent on the number of DNA lesions and required ATP. Chromatin assembly linked to the repair of single-strand breaks was disrupted by depletion of PCNA from a cell-free system. This defect was rescued by complementation with recombinant PCNA, arguing for role of PCNA in mediating chromatin assembly linked to DNA repair. We discuss the importance of the PCNA-CAF-1 interaction in the context of DNA damage processing and checkpoint control.  (+info)

Distinct importin recognition properties of histones and chromatin assembly factors. (8/113)

Synthesis of the protein components of nuclear chromatin occurs in the cytoplasm, necessitating specific import into the nucleus. Here, we report the binding affinities of the nuclear localisation sequence (NLS)-binding importin subunits for a range of histones and chromatin assembly factors. The results suggest that import of histones to the nucleus may be mediated predominantly by importin beta1, whereas the import of the other components probably relies on the conventional alpha/beta1 import pathway. Differences in recognition by importin beta1 were observed between histone H2A and the variant H2AZ, as well as between histone H3/4 with or without acetylation. The results imply that different histone variants may possess distinct nuclear import properties, with acetylation possibly playing an inhibitory role through NLS masking.  (+info)

*CHAF1A

Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene. Chromatin assembly factor I ( ... "Entrez Gene: CHAF1A chromatin assembly factor 1, subunit A (p150)". Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, ... interaction between chromatin assembly factor 1 and HP1 proteins". Mol. Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00)80204-X ... "The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1". Mol. Cell. Biol. 23 (9): ...

*CHAF1B

Chromatin assembly factor 1 subunit B is a protein that in humans is encoded by the CHAF1B gene. Chromatin assembly factor I ( ... "Entrez Gene: CHAF1B chromatin assembly factor 1, subunit B (p60)". Mello JA, Silljé HH, Roche DM, Kirschner DB, Nigg EA, ... Nabatiyan A, Krude T (Apr 2004). "Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of ... Shim KS, Bergelson JM, Furuse M, Ovod V, Krude T, Lubec G (2004). "Reduction of chromatin assembly factor 1 p60 and C21orf2 ...

*RSF1

"Functional analysis of the subunits of the chromatin assembly factor RSF". Mol Cell Biol. 23 (19): 6759-68. doi:10.1128/MCB. ... Remodeling and spacing factor 1 is a protein that in humans is encoded by the RSF1 gene. HBXAP is involved in transcription ... "Entrez Gene: RSF1 remodeling and spacing factor 1". Lee TH, Elledge SJ, Butel JS (1995). "Hepatitis B virus X protein interacts ... LeRoy G, Orphanides G, Lane WS, Reinberg D (1998). "Requirement of RSF and FACT for transcription of chromatin templates in ...

*TLK1

Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068- ... are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM] ... Sen SP, De Benedetti A (2006). "TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1". BMC Molecular ...

*Proliferating cell nuclear antigen

"Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function". Molecular and ... Chromatin remodeling factor • Histone acetyltransferase • Histone deacetyltransferase • DNA methyltransferase • Sister- ... chromatid cohesion factors • Protein kinases • Cell-cycle regulators • Apoptotic factors for details see PCNA has been shown to ... PCNA is clamped to DNA through the action of replication factor C (RFC), which is a heteropentameric member of the AAA+ class ...

*CSTF3

"Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1". Mol. Cell. 7 (1): 13-20. doi: ... cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in ... Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene. The protein encoded by ... Takagaki Y, Manley JL (1992). "A human polyadenylation factor is a G protein beta-subunit homologue". J. Biol. Chem. 267 (33): ...

*TLK2

Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. These are different ... 202 (1-2): 193-201. doi:10.1016/S0378-1119(97)00495-2. PMID 9427565. Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA ( ...

*CBX1

... interaction between chromatin assembly factor 1 and HP1 proteins". Molecular Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00) ... a potential role in the regulation of higher-order chromatin structure". The Journal of Pathology. 196 (2): 135-44. doi:10.1002 ... 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse ... Chromobox protein homolog 1 is a protein that in humans is encoded by the CBX1 gene. The protein is localized at ...

*CBX5 (gene)

... interaction between chromatin assembly factor 1 and HP1 proteins". Molecular Cell. 4 (4): 529-40. doi:10.1016/S1097-2765(00) ... "The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1". Molecular and Cellular ... "Selective interaction between the chromatin-remodeling factor BRG1 and the heterochromatin-associated protein HP1alpha". The ... "Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis". ...

*MBD1

"The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 23 (9): ... "The Methyl-CpG Binding Protein MBD1 Interacts with the p150 Subunit of Chromatin Assembly Factor 1". Mol. Cell. Biol. 23 (9): ... Assembly of novel secondary chromatin structures in the absence of DNA methylation". J. Biol. Chem. 278 (34): 32181-8. doi: ... Ueba T, Kaspar B, Zhao X, Gage FH (1999). "Repression of human fibroblast growth factor 2 by a novel transcription factor". J. ...

*ASF1B

Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Transcriptional regulation of human chromatin assembly factor ASF1". DNA and Cell Biology. 26 (2): 91-9. doi:10.1089/dna. ... in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. ... Sen SP, De Benedetti A (2006). "TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1". BMC Molecular ...

*ASF1A

It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair ... Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases ... "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068- ... Munakata T, Adachi N, Yokoyama N, Kuzuhara T, Horikoshi M (Mar 2000). "A human homologue of yeast anti-silencing factor has ...

*Deficiency of RbAp48 protein and memory loss

Proteins RbAp48 is a key player in the assembly of nucleosomes. RbAp48 protein is a subunit of the chromatin-assembly factor-1 ... a human cell factor required for chromatin assembly during DNA replication in vitro". Cell. 58 (1): 15-25. doi:10.1016/0092- ... Chromatin function and acetylation A direct link between chromatin function and acetylation was established by the discovery ... interacts with H3-H4 dimers and imply that the function of RbAp48 is involved in numerous process such as chromatin assembly, ...

*TOP3A

... and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1". ... 256 (1): 225-36. doi:10.1006/excr.1999.4778. PMID 10739669. Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, ... 75 (1): 104-8. doi:10.1002/(SICI)1096-8628(19980106)75:1. 3.0.CO;2-P. PMID 9450867. "Entrez Gene: TOP3A topoisomerase (DNA) III ...

*Bloom syndrome protein

... and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1". ... 46 (1): 43-53. doi:10.1016/j.molcel.2012.02.020. PMC 3328772 . PMID 22500736. Séguéla-Arnaud M, Crismani W, Larchevêque C, ... Sharma S, Sommers JA, Wu L, Bohr VA, Hickson ID, Brosh RM (March 2004). "Stimulation of flap endonuclease-1 by the Bloom's ... doi:10.1016/0092-8674(95)90105-1. PMID 7585968. German J, Roe AM, Leppert MF, Ellis NA (1994). "Bloom syndrome: an analysis of ...

*Eukaryotic DNA replication

The heterotrimeric chaperone chromatin assembly factor 1 (CAF-1) is a chromatin formation protein that is involved in ... "Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function". Mol. Cell. Biol. 29 ( ... The assembly of the Mcm proteins onto chromatin requires the coordinated function of the Origin Recognition Complex (ORC), Cdc6 ... When the ORC binds to DNA at replication origins, it then serves as a scaffold for the assembly of other key initiation factors ...

*DBF4

"The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating ... Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in ... "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. ... 327 (1): 111-28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612. Jiang W, McDonald D, Hope TJ, Hunter T (Oct 1999). " ...

*CAF-1

Kaufman PD, Kobayashi R, Kessler N, Stillman B (1995). "The p150 and p60 subunits of chromatin assembly factor I: a molecular ... Smith S, Stillman B (1989). "Purification and characterization of CAF-I, a human cell factor required for chromatin assembly ... Hoek, M.; Stillman, B. (2003). "Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in ... chromatin assembly factor-1) is a complex, including Chaf1a (p150), Chaf1b (p60) and p50 subunits that assembles histone ...

*Spindle apparatus

Makde R, England J, Yennawar H, Tan S (2010). "Structure of the RCC1 chromatin factor bound to the nucleosome core particle". ... The gradient triggers release of spindle assembly factors (SAFs) from inhibitory interactions via the transport proteins ... and yeast are required for proper spindle assembly; in mammals, CLASP1 and CLASP2 both contribute to proper spindle assembly ... Thus, a gradient of GTP-bound Ran is generated around the vicinity of mitotic chromatin. Glass beads coated with RCC1 induce ...

*WAC protein domain

ACF (for ATP-utilising chromatin assembly and remodeling factor) is a chromatin-remodeling complex that catalyzes the ATP- ... ATP-dependent chromatin assembly factor large subunit Acf1 human - WSTF (Williams syndrome transcription factor) mouse - cbp146 ... The function of the WAC domain remains to be fully elucidated but it is thought to be part of the process of chromatin assembly ... "Binding of Acf1 to DNA involves a WAC motif and is important for ACF-mediated chromatin assembly". Mol. Cell. Biol. 22 (18): ...

*Bruce William Stillman

... to study DNA replication-coupled chromatin assembly in a test tube and discovered proteins such as Chromatin Assembly Factor-1 ... The proteins that combine with DNA to organize the genome into a chromatin structure include histones. He developed a ... These studies resulted in understanding how chromatin is inherited. Commonwealth Postgraduate Award (1976-1978) Damon Runyon- ... "Reconstitution of complete SV40 DNA replication with purified replication factors". Journal of Biological Chemistry. 269 (14): ...

*CAF

... chromatin assembly factor-1), a protein complex maintaining chromatin Central African Republic, by ISO 3166-1 country code ...

*Phycodnaviridae

Four transcription factor-like proteins have been found in PBSV-1, including TFIIB (A107L), TFIID (A552R), TFIIS (A125L), and a ... The second phase of proteins are translated in the cytoplasm and the assembly of virus capsids begins about 2-3 hours post ... The HaV virus, infecting H. akashiwo has been shown to be a factor in bloom termination. Suttle et al. (1990) suggested that ... The eukaryotic replication factor C". ResearchGate. Retrieved 2017-03-03. "Characterization of an ATP-dependent DNA ligase ...

*Chromatin

Protocol for in vitro Chromatin Assembly ENCODE threads Explorer Chromatin patterns at transcription factor binding sites. ... Other factors are probably involved, such as the association and dissociation of transcription factor complexes with chromatin ... In eukaryotes, ATP dependent chromatin remodeling complexes and histone-modifying enzymes are two predominant factors employed ... When the chromatin decondenses, the DNA is open to entry of molecular machinery. Fluctuations between open and closed chromatin ...

*Nucleosome

They are assembled by the chromatin assembly factor-1 (CAF-1) complex, which consists of three subunits (p150, p60, and p48). ... "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA ... Yadav T, Whitehouse I (April 2016). "Replication-Coupled Nucleosome Assembly and Positioning by ATP-Dependent Chromatin- ... Newly synthesized H3 and H4 are assembled by the replication coupling assembly factor (RCAF). RCAF contains the subunit Asf1, ...

*Chromatin remodeling

Chromatin assembly factor-1) - histone chaperone that execute a coordinating role in сhromatin remodeling. Chen T, Dent SY ( ... The ISWI-family remodelers have been shown to play central roles in chromatin assembly after DNA replication and maintenance of ... Chromatin remodeling is the dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the ... Epigenetics Histone Nucleosomes Chromatin Histone acetyltransferase Transcription factors CAF-1 ( ...

*Endospore

Henriques AO, Moran CP Jr (2007). "Structure, assembly, and function of the spore surface layers". Annu Rev Microbiol. 61: 555- ... The core contains the spore chromosomal DNA which is encased in chromatin-like proteins known as SASPs (small acid-soluble ... The heat resistance of endospores is due to a variety of factors: *Calcium dipicolinate, abundant within the endospore, may ... These studies have contributed much to our understanding of the regulation of gene expression, transcription factors, and the ...

*HHV Latency Associated Transcript

... and demonstrated that the region insulated activated LAT chromatin from repressed chromatin that would otherwise produce the ... CCCTC-binding factor (CTCF) is a zinc finger protein which occurs naturally in some human cells. CTCF is localized to the ... "Herpesviral latency-associated transcript gene promotes assembly of heterochromatin on viral lytic-gene promoters in latent ... permanent dead link] Chen Q, Lin L, Smith S, Huang J, Berger SL, Zhou J (May 2007). "CTCF-Dependent Chromatin Boundary Element ...
DNA repair in the eukaryotic cell disrupts local chromatin organization. To investigate whether the resetting of nucleosomal arrays can be linked to the repair process, we developed model systems, with both Xenopus egg extract and human cell extracts, to follow repair and chromatin assembly in parallel on circular DNA templates. Both systems were able to carry out nucleotide excision repair of DNA lesions. We observed that UV-dependent DNA synthesis occurs simultaneously with chromatin assembly, strongly indicating a mechanistic coupling between the two processes. A complementation assay established that chromatin assembly factor I (CAF1) is necessary for this repair associated chromatin formation.. ...
We study proteins that regulate chromosome structure and function. We recently discovered that Chromatin Assembly Factor-1 (CAF-1) regulates protein accumulation and long-range DNA interactions at the nucleolus. (Smith et al., Mol. Biol. Cell 25: 2866-2881 (2014)). We are currently performing genome-scale analyses of the contributions of CAF-1 to human chromosome interactions and functions.
De novo chromatin assembly maintains histone density on the daughter strands in the wake of the replication fork. The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not essential for yeast cell proliferation. Depletion of CAF-1 in human cell lines demonstrated that CAF-1 was required for efficient progression through S-phase. Cells lacking CAF-1 accumulated in early and mid S-phase and replicated DNA slowly. The checkpoint kinase Chk1, but not Chk2, was phosphorylated in response to CAF-1 depletion, consistent with a DNA replication defect. CAF-1-depleted cell extracts completely lacked DNA replication-coupled chromatin assembly activity, suggesting that CAF-1 is required for efficient S-phase progression in human cells. These results indicate that, in contrast to yeast, human CAF-1 is necessary for coupling chromatin assembly with DNA replication.. ...
We have shown for the first time that the function of CAF-1 is important for viability following double-strand DNA repair. Furthermore, CAF-1 functions genetically in both homologous recombination and NHEJ, and its function during double-strand DNA repair is dependent on the interaction between CAF-1 and PCNA. Given the biochemical role of CAF-1 in DNA synthesis-coupled chromatin assembly, we propose that CAF-1 mediates chromatin assembly during double-strand DNA repair in vivo.. We have observed sensitivity of CAF-1 mutants to a variety of double-strand DNA-damaging agents. A systematic analysis of the yeast deletion collection also isolated the cac2Δ as a mutant sensitive to MMS (Chang et al. 2002). Similarly, mutants of the genes encoding Arabidopsis CAF-1, fas1, and fas2 are sensitive to MMS (Takeda et al. 2004). Given the role of CAF-1 in chromatin assembly during NER of single-strand lesions and the sensitivity of CAF-1 mutants to UV irradiation (Gaillard et al. 1996; Kaufman et al. 1997; ...
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
Shop ATP synthase assembly factor ELISA Kit, Recombinant Protein and ATP synthase assembly factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Online ISSN 1469-3178. Copyright© 2018 EMBO. This website is best viewed using the latest versions of all modern web browsers. Older browsers may not display correctly.. ...
KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere InactivationKAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation ...
Abstract: To study the relation between chromatin structure and DNA function in detail it is necessary to have an in vitro procedure for assembling nucleosomes on a naked DNA template with properties similar to native chromatin. Such procedures exist for yeast and animal model systems but have not been developed for plants. The goal of this project was to lay the groundwork for developing a chromatin assembly extract from plants. Extracts from various plant materials were tested to determine their suitability for chromatin reconstitution. Tissues from plants are thought to have much higher levels of protease and nuclease activities than those of animals or yeast. Therefore, methods to determine the relative activity of proteases and nucleases had to be developed to determine if the template DNA, histones, and chromatin assembly proteins could survive the chromatin assembly reaction. Additionally, methods to streamline the isolation of maize nuclei and purification of histones were developed. ...
Chaf1b - Chaf1b (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b) available for purchase from OriGene - Your Gene Company.
Figure 4. CAF-1 binds (H3-H4)2 tetramers and H3-H4 dimers with similar affinities, yet 2-fold different stoichiometries. (A) An overlay of the binding curves for CAF-1 titrated into a H3(L126R, I130R)-H4 double mutant that effectively prevents tetramer formation (gray squares) and a cross-linked (H3-H4)2 tetramer (triangle) with the wild-type H3-H4 curve from Figure 1 (dashed line). The curves are very similar to wild-type and produce dissociation constants of 5.5 nM for the double mutant and 9.0 nM for the cross-linked complex (Table 1). (B) Determination of the stoichiometry of the CAF-1•WT H3-H4 complex by titration of CAF-1 into a constant concentration of labeled H3-H4. Fluorescence change occurs until wild-type H3-H4 is saturated with CAF-1 and the ratio at this inflection point is equal to the number of CAF-1 molecules bound to a single H3-H4 complex. (C) Determination of the stoichiometry of CAF-1/H3(L126R, I130R)-H4 complex. The fluorescence change plateaus similar to that of ...
Genetic information processingDNA metabolismDNA replication, recombination, and repairCDK-activating kinase assembly factor MAT1 (TIGR00570; HMM-score: 7) ...
Two main chromatin assembly pathways ensure the proper transmission of chromatin organization and chromatin-based information throughout the cell cycle. A replication-dependent (RD) pathway that couples chromatin assembly to DNA synthesis and a replication-independent (RI) pathway. Whether these pathways contribute to the establishment of chromatin domains like heterochromatin or euchromatin by introducing modifications on histones or modulating chromatin structure remains unknown. Using Xenopus laevis egg extracts we monitored RD and RI chromatin assembly on single-stranded and double-stranded DNA templates. Even though RD assembly proceeded faster than RI assembly the histone content and saturation level with nucleosomes were similar. Despite these comparable topological features, the hydrodynamic behavior of both chromatin species in sucrose gradient centrifugation clearly differed. The RD assembled chromatin ran at lower sucrose concentrations than the RI created chromatin suggesting ...
In the eukaryotic nucleus, hundreds of millions of base pairs of DNA are packed into chromosomes. Chromatin, the central nucleoprotein filament of a chromosome, has many different forms and organization levels. Chromatin is the natural state of DNA in the nucleus and the native substrate for nuclear reactions such as DNA replication, recombination, repair and transcription. The assembly of DNA into chromatin and dynamic conversion between its different forms are critical steps in the maintenance and regulation of the eukaryotic genome. The ultimate goal of our research is to understand how chromosomes are assembled and how chromatin assembly regulates the structure and activity of eukaryotic chromosomes. The crucial first step in this direction is a systematic study of factors that mediate this process. To this end we use biochemical approaches to analyze mechanisms of chromatin assembly by histone chaperones and ATP-dependent enzymes. We also dissect their function in vivo by methods of ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Shop ATP synthase mitochondrial F1 complex assembly factor ELISA Kit, Recombinant Protein and ATP synthase mitochondrial F1 complex assembly factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Signaling cascades are essential in instructing cell proliferation and differentiation during animal development and are often manifested by turning on or shutting off the expression of a group of genes. The specificity of different signaling pathways is usually determined by activating specific transcription factors that bind to the enhancers of their target genes. However, in eukaryotic cells the full transcription of a particular target gene is also dependent on the recruitment of chromatin remodelers and histone modifiers, in addition to transcription factors, in order to ensure a local chromatin environment that is permissive for the access of a complete set of regulatory proteins. Although CAF-1 was initially identified as a histone chaperone for DNA synthesis-coupled chromatin assembly (Smith and Stillman, 1989; Das et al., 2009), it is becoming increasingly evident that CAF-1 has functions in the regulation of other cellular and developmental processes, such as heterochromatin formation ...
This event is part of the Condensed Matter Theory Seminar Series. Abstract: Adsorption-desorption processes are widespread in physics and biology. The 1D case for hard-exclusive particles has been studied extensively and exhibits transient "jamming" followed by extremely slow equilibration. However, in many realistic applications particles are somewhat compressible. We show that this profoundly alters the kinetics: the "jamming" plateau is replaced by a "cramming" phase, and the subsequent relaxation is very fast and can even be non-monotonic. As a specific example we consider the assembly kinetics of nucleosomes on eukaryotic DNA. This biological system also involves the physics of disordered systems and glassy dynamics.. ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
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The essential histone H3 variant Cse4 plays a crucial role at the centromere in S. cerevisiae, where it replaces histone H3 in that it assembles centromere specific (Cse4-H4)2 tetrameres. We found in our study that the histone H3 variant was able to interact over its unique N-Terminus with two subunits of the histone acetyltransferase complex SAS-I: Sas2 and Sas4. Mutations within the acetyl-CoA binding site (HAT domain) or the zink-finger of Sas2 disrupted the binding to Cse4, although an indirect interaction was found with co-immunoprecipitation experiments. Additionally, the N-terminus of Cse4 interacted with Cac1, the largest subunit of the chromatin assembly factor CAF-I and Asf1 - two histone chaperones that assemble histones H3 and H4 into nucleosomes. Our findings further suggest a role of Cac1 independent of Cac2 and Cac3 as no binding to Cse4 could be detected. A role for Sas2 at the centromere was further confirmed in that a sas2 deletion (sas2 delta) disrupted the binding of Cse4 to ...
Stuffing the long strands of chromosomal DNA into a eukaryotic nucleus requires that the DNA be compacted in length approximately 10,000 to 50,000 -fold. Incredibly, cells achieve this tight packing of the DNA while still maintaining the chromosomes in a form that allows regulatory proteins to gain access to the DNA to turn on (or off) specific genes or to duplicate the chromosomal DNA (replication). This engineering feat is accomplished by a variety of chromatin proteins, the most abundant of which are the histones. Histones associate with DNA to accomplish the first step in chromatin assembly, forming protein-DNA structures known as nucleosomes. When viewed under the electron microscope, nucleosomes are beads of ~10 nm in diameter that are distributed along the ~2 nm DNA string (Kornberg, 1974; Olins and Olins, 1974) about once every 200 bp. Each bead is a nucleosome core particle (install the plug-in to view this link) that includes ~146 bp of DNA wrapped almost twice around a core histone ...
The anti-silencing function protein 1 (Asf1) is a chaperone that forms a complex with histones H3 and H4 facilitating dimer deposition and removal ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
Complete information for ASF1A gene (Protein Coding), Anti-Silencing Function 1A Histone Chaperone, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
RBBP7 Full-Length MS Protein Standard (NP_002884), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
Kinderparacetamol SDG is a medicine available in a number of countries worldwide. A list of US medications equivalent to Kinderparacetamol SDG is available on the Drugs.com website.
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5 ...
Previous work has revealed important roles for HIRA in myogenesis. It is important for the differentiation of C2C12 myoblasts into myotubes (Song et al., 2012). HIRA facilitates expression of Myod1 through incorporation of histone H3.3 into its promoter and enhancer regions (Yang et al., 2011b). Histone H3.3 itself epigenetically maintains expression of the Myod1 gene after cell division (Ng and Gurdon, 2008). HIRA cooperates with MEF2 transcription factors to drive expression of MEF2 target genes, like Myog (Yang et al., 2011a). HIRA is also a substrate for AKT proteins. HIRA phosphorylation by AKT proteins in myoblasts preserves the proliferative state. Dephosphorylation occurs as myoblasts differentiate towards myotubes (Yang et al., 2016). Thus, HIRA-mediated chromatin assembly is critical during myogenesis. In this study, we expanded our understanding of the function of HIRA in skeletal muscle by determining the consequence of its loss after myogenesis in the homeostasis and function of ...
Taken together, these studies will reveal how the transcriptional machinery competes with chromatin assembly to regulate transcription, not only during genome activation but potentially also during differentiation and reprogramming.. 2. The role of chromatin structure and nuclear architecture in genome activation. Chromatin regulates the accessibility of the genome for DNA binding proteins. Changes in chromatin structure and nuclear organization are thus critical to understanding how regions of the genome become transcriptionally competent. We have previously shown that dramatic changes in chromatin structure accompany the onset of zygotic gene expression (Vastenhouw et al., Nature 2010 PMID: 20336069; Zhang et al., GR 2014 PMID: 24285721). Thus, the onset of transcription during genome activation provides a powerful system to study the relationship between chromatin structure and transcriptional activity.. We have recently found that transcriptional activity and accumulation of the produced RNA ...
Booth DG, Beckett AJ, Molina O, Samejima I, Masumoto H, Kouprina N, Larionov V, Prior IA, Earnshaw WC. 3D-CLEM Reveals that a Major Portion of Mitotic Chromosomes Is Not Chromatin. Mol Cell. 2016 Nov 17;64(4):790-802. doi: 10.1016/j.molcel.2016.10.009. Epub 2016 Nov 10. PMID: 27840028; FREE ARTICLE. Ohzeki J, Shono N, Otake K, Martins NM, Kugou K, Kimura H, Nagase T, Larionov V, Earnshaw WC, Masumoto H. KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation. Dev Cell. 2016 Jun 6;37(5):413-27. doi: 10.1016/j.devcel.2016.05.006. PMID: 27270040; FREE ARTICLE. Ohta S, Montaño-Gutierrez LF, de Lima Alves F, Ogawa H, Toramoto I, Sato N, Morrison CG, Takeda S, Hudson DF, Rappsilber J, Earnshaw WC. Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes. Mol Cell Proteomics. 2016 Aug;15(8):2802-18. doi: 10.1074/mcp.M116.057885. Epub 2016 May 26. PMID: 27231315; ...
The centromeric histone CENP-A is incorporated at different cell cycle phases during somatic mitosis, meiosis I and meiosis II in Drosophila melanogaster.
The chromo domain was originally identified as a protein sequence motif common to the Drosophila chromatin proteins, Polycomb (Pc) and heterochromatin protein 1 [HP1; Paro and Hogness (1991) Proc. Natl. Acad. Sci. USA, 88, 263-267; Paro (1990) Trends Genet., 6, 416-421]. Here we describe a second chromo domain-like motif in HP1. Subsequent refined searches identified further examples of this chromo domain variant which all occur in proteins that also have an N-terminally located chromo domain. Due to its relatedness to the chromo domain, and its occurrence in proteins that also have a classical chromo domain, we call the variant the chromo shadow domain. Chromo domain-containing proteins can therefore be divided into two classes depending on the presence, for example in HP1, or absence, for example in Pc, of the chromo shadow domain. We have also found examples of proteins which have two classical chromo domains. The Schizosaccharomyces pombe SWI6 protein, involved in repression of the silent ...
Create a Notification; as soon as the NotificationJobs finds some NotificationTask to execute, execution gets persisted but NotificationTask.getExecs() is empty.. Possibly related to ...

The Yeast Histone Chaperone Chromatin Assembly Factor 1 Protects Against Double-Strand DNA-Damaging Agents | GeneticsThe Yeast Histone Chaperone Chromatin Assembly Factor 1 Protects Against Double-Strand DNA-Damaging Agents | Genetics

1996 Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I. Cell 86: 887-896. ... Kaufman, P. D., R. Kobayashi, N. Kessler and B. Stillman, 1995 The p150 and p60 subunits of chromatin assembly factor I: a ... Myung, K., V. Pennaneach, E. S. Kats and R. D. Kolodner, 2003 Saccharomyces cerevisiae chromatin-assembly factors that act ... Prado, F., F. Cortes-Ledesma and A. Aguilera, 2004 The absence of the yeast chromatin assembly factor Asf1 increases genomic ...
more infohttp://www.genetics.org/content/171/4/1513

Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins  - CSHL Scientific...Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins - CSHL Scientific...

We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 ... Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins ... Interaction between Chromatin Assembly Factor 1 and HP1 Proteins. Molecular Cell, 4 (4). pp. 529-540. ... The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. ...
more infohttp://repository.cshl.edu/25029/

Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo  - CSHL Scientific Digital...Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo - CSHL Scientific Digital...

The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not ... Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo ... Hoek, M., Stillman, B. (October 2003) Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA ... De novo chromatin assembly maintains histone density on the daughter strands in the wake of the replication fork. ...
more infohttp://repository.cshl.edu/id/eprint/24988/

Paul Kaufman, Ph.D.Paul Kaufman, Ph.D.

Cell 25: 2866-2881 (2014)). We are currently performing genome-scale analyses of the contributions of CAF-1 to human chromosome ... We recently discovered that Chromatin Assembly Factor-1 (CAF-1) regulates protein accumulation and long-range DNA interactions ... For example, we discovered that Chromatin Assembly Factor-1 (CAF-1) regulates protein accumulation and long-range DNA ... CAF-1 also regulates the protein composition of human mitotic chromosomes. These novel activities are mediated via regulation ...
more infohttps://www.umassmed.edu/mccb/faculty-MCCB/faculty-MCCB/faculty-profile-pages/kaufman-paul/

Chromatin Protocols - University of Manitoba LibrariesChromatin Protocols - University of Manitoba Libraries

Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos. * Analysis of Reconstituted Chromatin Using a ... Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo ... Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a ... Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a ...
more infohttp://link.lib.umanitoba.ca/portal/Chromatin-Protocols-edited-by-Srikumar-P./Z07dyPRHEi4/

Centromere domain organization and histone modificationsCentromere domain organization and histone modifications

It is becoming increasingly clear that the key factors for assembly and function of the centromere structure are the ... specialized histories and modified histones which are present in the centromeric heterochromatin and in the chromatin of the ... Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid ...
more infohttp://sh.diva-portal.org/smash/record.jsf?pid=diva2:508338

Chromatin assembly factor 1 subunit A, C-terminal (IPR029105) | InterPro | EMBL-EBIChromatin assembly factor 1 subunit A, C-terminal (IPR029105) | InterPro | EMBL-EBI

Chromatin assembly factor 1 subunit A (CAF1 subunit A or subunit p150) is a core component of the CAF-1 complex, a complex ... Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in ... The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.. Mol. Cell. Biol. 23 3226- ... Chromatin assembly factor 1 subunit A, C-terminal (IPR029105). Short name: CAF1-p150_C2 ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR029105

Chromatin assembly factor 1, subunit B - Tetraodon nigroviridis (Spotted green pufferfish)Chromatin assembly factor 1, subunit B - Tetraodon nigroviridis (Spotted green pufferfish)

Chromatin assembly factor 1, subunit BImported. ,p>Information which has been imported from another database using automatic ... tr,H3BYY5,H3BYY5_TETNG Chromatin assembly factor 1, subunit B OS=Tetraodon nigroviridis OX=99883 PE=4 SV=1 ... SSF50978 SSF50978, 1 hit. PROSITEi. View protein in PROSITE. PS00678 WD_REPEATS_1, 1 hit. PS50082 WD_REPEATS_2, 3 hits. ... H3BYY5-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MKVVTCEIAW HNKEPVYSLD FQHGCDGRLH RLATAGVDTA ...
more infohttps://www.uniprot.org/uniprot/H3BYY5

RCSB PDB - Protein Feature View 









 - Chromatin assembly factor 1 subunit B - Q13112 (CAF1B HUMAN)RCSB PDB - Protein Feature View - Chromatin assembly factor 1 subunit B - Q13112 (CAF1B HUMAN)

... and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon ... Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto ... histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. UniProt ... Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of ...
more infohttp://www.rcsb.org/pdb/protein/Q13112

chromatin assembly factor 1 subunit B ELISA Kits | Biocompare.comchromatin assembly factor 1 subunit B ELISA Kits | Biocompare.com

Compare chromatin assembly factor 1 subunit B ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... chromatin assembly factor 1 subunit B ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ... Your search returned 47 chromatin assembly factor 1 subunit B ELISA ELISA Kit across 6 suppliers. ... THP-1 cells were treated with LPS for 24 hours and treated with a TAK1 inhibitor. The ELISA kit was ... read more ...
more infohttps://www.biocompare.com/pfu/110627/soids/2-2265334/ELISA_Kit/ELISA_chromatin_assembly_factor_1_subunit_B

CHAF1A - Chromatin assembly factor 1 subunit A - Homo sapiens (Human) - CHAF1A gene & proteinCHAF1A - Chromatin assembly factor 1 subunit A - Homo sapiens (Human) - CHAF1A gene & protein

... a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating ... histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A ... CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating ... Chromatin assembly factor 1 subunit AAdd BLAST. 956. Amino acid modifications. Feature key. Position(s). DescriptionActions. ...
more infohttp://www.uniprot.org/uniprot/Q13111

Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in...Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in...

Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ... Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in ...
more infohttp://www.genetics.org/content/211/4/1219

Chromatin assembly factor 1 subunit B | definition of chromatin assembly factor 1 subunit B by Medical dictionaryChromatin assembly factor 1 subunit B | definition of chromatin assembly factor 1 subunit B by Medical dictionary

What is chromatin assembly factor 1 subunit B? Meaning of chromatin assembly factor 1 subunit B medical term. What does ... Looking for online definition of chromatin assembly factor 1 subunit B in the Medical Dictionary? chromatin assembly factor 1 ... which is thought to mediate chromatin assembly in DNA replication and DNA repair. Chromatin assembly factor 1, subunit B, is a ... redirected from chromatin assembly factor 1 subunit B) CHAF1B. A gene on chromosome 21q22.13 that encodes chromatin assembly ...
more infohttp://medical-dictionary.thefreedictionary.com/chromatin+assembly+factor+1+subunit+B

Interaction between the DrosophilaCAF-1 and ASF1 Chromatin Assembly Factors | Molecular and Cellular BiologyInteraction between the DrosophilaCAF-1 and ASF1 Chromatin Assembly Factors | Molecular and Cellular Biology

1996) Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I. Cell 86:887-896. ... 1996) Post-replicative chromatin assembly by Drosophila and human chromatin assembly factor-1. Mol. Cell. Biol. 16:810-817. ... 1997) RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Genes Dev. 11 ... such as ATP-utilizing chromatin assembly and remodeling factor (ACF), which catalyzes the assembly of histones into periodic ...
more infohttps://mcb.asm.org/content/21/19/6574?ijkey=52b01934b6bf9c441759d33a7639a770caba32e7&keytype2=tf_ipsecsha

Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch...Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch...

Chromatin assembly factor 1 (CAF1), a histone chaperone that mediates the deposition of histone H3/H4 onto newly synthesized ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ... Drosophila chromatin assembly factor 1 p105 and p180 subunits are required for follicle cell proliferation via inhibiting Notch ...
more infohttp://jcs.biologists.org/content/early/2019/01/09/jcs.224170?download=true

The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. |...The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. |...

The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein.. J K Tyler ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ... The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein. ...
more infohttps://mcb.asm.org/content/16/11/6149?ijkey=5657a0fa742359094b8c107f9828e4ef0985a8e3&keytype2=tf_ipsecsha

CHAF1A Gene - GeneCards | CAF1A Protein | CAF1A AntibodyCHAF1A Gene - GeneCards | CAF1A Protein | CAF1A Antibody

Chromatin Assembly Factor 1 Subunit A, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Chromatin assembly factor I p150 subunit (CAF1A_HUMAN). *Chromatin assembly factor 1, subunit A (P150), isoform CRA_a (D6W625_ ... Chromatin assembly factor 1 subunit A. Protein Accession:. Q13111. Secondary Accessions: *Q6NXG5 ... CHAF1A (Chromatin Assembly Factor 1 Subunit A) is a Protein Coding gene. Among its related pathways are Autophagy Pathway and ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=10036

Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1<...Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1<...

... chromatin assembly factor I; p300-CBP Transcription Factors, 2.3.1.48; p300-CBP-associated factor, 2.3.1.48 參考文獻: Kaufman, P.D ... chromatin assembly factor I; p300-CBP Transcription Factors, 2.3.1.48; p300-CBP-associated factor, 2.3.1.48 參考文獻: Kaufman, P.D ... It is essential for coupling nucleosome assembly to newly synthesized DNA. Whether chromatin assembly factor 1 subunits have ... It is essential for coupling nucleosome assembly to newly synthesized DNA. Whether chromatin assembly factor 1 subunits have ...
more infohttps://tmu.pure.elsevier.com/en/publications/gene-specific-transcriptional-activation-mediated-by-the-p150-sub

Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells  - CSHL Scientific...Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells - CSHL Scientific...

Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells ... The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been ... Such chromatin recruitment resembles that seen for PCNA, a DNA replication and repair factor. The chromatin-associated fraction ... Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells. Journal of Cell ...
more infohttp://repository.cshl.edu/29916/

Chromatin Proteins and Chromatin Structure in Spermatogenesis | SpringerLinkChromatin Proteins and Chromatin Structure in Spermatogenesis | SpringerLink

... a physiological nucleosome assembly factor. EMBO J. 3, 1193-1199.PubMedGoogle Scholar ... RYOJI, M. and WORCEL, A. (1984). Chromatin assembly in Xenopus oocytes: in vivo studies. Cell 37, 21-32.PubMedCrossRefGoogle ... GLIKIN, G.C., RUBERTI, I., WORCEL, A. (1984). Chromatin assembly in Xenopus oocytes: in vitro studies. Cell 37, 33-41.PubMed ... Mezquita C. (1985) Chromatin Proteins and Chromatin Structure in Spermatogenesis. In: Reeck G.R., Goodwin G.H., Puigdomènech P ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-7615-6_20

An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore.  - PubMed - NCBIAn enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. - PubMed - NCBI

... chromatin assembly factor); (J) mTagBFP2-Rab5a-7; (K) mTagBFP2-α-tubulin-18; (L) mTagBFP2-myosin-IIA-18; (M) mTagBFP2-PCNA-19 ... Subach OM1, Cranfill PJ, Davidson MW, Verkhusha VV.. Author information. 1. Department of Anatomy and Structural Biology, and ... Bands 1 and 2 show the total polypeptide chain and the polypeptide chain after cleavage inside the chromophore, respectively. ( ... D) The ratio of the band 1 intensity to the band 2 intensity for SDS-PAGE analysis presented in . (E) Deconvoluted FT-ICR mass ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/22174863?dopt=Abstract

Molecules  | Free Full-Text | Pharmacogenomics of Scopoletin in Tumor Cells | HTMLMolecules | Free Full-Text | Pharmacogenomics of Scopoletin in Tumor Cells | HTML

Scopoletin activated NF-κB in a SEAP-driven NF-κB reporter cell line, indicating that NF-κB might be a resistance factor for ... although NF-κB activation may be considered as resistance factor for this compound. Further investigations are warranted to ... which all harbored binding motifs in their promoter sequences for the transcription factor, NF-κB, which is known to be ... Chromatin assembly factor 1, subunit A (p150). Chromatin assembly in DNA replication and DNA repair. ...
more infohttps://www.mdpi.com/1420-3049/21/4/496/htm

Juan LJ[au] - PubMed - NCBIJuan LJ[au] - PubMed - NCBI

Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1. ... Host-viral effects of chromatin assembly factor 1 interaction with HCMV IE2. ... Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores. ... Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Juan+LJ%5Bau%5D&dispmax=50

Histone-binding protein RBBP4, N-terminal (IPR022052) | InterPro | EMBL-EBIHistone-binding protein RBBP4, N-terminal (IPR022052) | InterPro | EMBL-EBI

RBBP4 is a subunit of the chromatin assembly factor 1 (CAF-1) complex. The CAF-1 complex is a conserved heterotrimeric protein ... specifically it facilitates replication-dependent nucleosome assembly with the major histone H3 (H3.1). This domain is an alpha ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR022052

CAF-1 | definition of CAF-1 by Medical dictionaryCAF-1 | definition of CAF-1 by Medical dictionary

Looking for online definition of CAF-1 in the Medical Dictionary? CAF-1 explanation free. What is CAF-1? Meaning of CAF-1 ... A gene on chromosome 19p13.3 that encodes chromatin assembly factor 1, subunit A, a core component of the Chromatin Assembly ... which is thought to mediate chromatin assembly in DNA replication and DNA repair.. ... CAF-1 , definition of CAF-1 by Medical dictionary https://medical-dictionary.thefreedictionary.com/CAF-1 ...
more infohttps://medical-dictionary.thefreedictionary.com/CAF-1
  • The aim of this project is to combine powerful genetic, biochemical, genomic and systems biology approaches available in the model bacterium Bacillus subtilis with modern high-resolution light microscopic techniques to study the dynamics and mechanistic details of the MreB cytoskeleton and of CW assembly. (europa.eu)
  • To test this prediction, genomic bisulphite sequencing was carried out on 473 bp of the H19 imprinting control region in DNA obtained from midgestation F(1) hybrid mouse embryos (C57BL/6 Ã Mus musculus castaneus ) exposed to ethanol during preimplantation development. (las.ac.cn)
  • The double-stranded DNA genome of herpes simplex virus 1 (HSV-1) associates with histones to form chromatin rapidly following infection of a host cell, and the resulting chromatin structure is dynamically regulated throughout productive HSV-1 infection (1-3). (asm.org)
  • CAF-1 has also been implicated in single-strand DNA repair via the nucleotide excision repair (NER) pathway. (genetics.org)
  • We suggest that berberine and camptothecin inhibit factors that might be required for leaf adaxial cell differentiation through a pathway independent of AS1 and AS2. (nii.ac.jp)
  • This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). (cellsignal.com)
  • The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been investigated after UV irradiation of HeLa cells. (cshl.edu)
  • Nucleosomal DNAs assembled or modified by different chromatin remodeling enzymes differentially impact the origin licensing and helicase activation steps of replication initiation. (elifesciences.org)
  • This process is highly related to the linear topography of different chromatin states and to the three-dimensional (3D) organization of the genome, which defines territories such as euchromatic and heterochromatic domains. (biomedcentral.com)
  • In Arabidopsis thaliana , CAF-1 is important for the stable maintenance of gene expression states at shoot and root apical meristems ( 19 ). (asm.org)
  • IMPORTANCE It is becoming increasingly appreciated that regulation of the state of chromatin is a major determinant in control of gene expression. (asm.org)
  • This work also further establishes HSV-1 infection as a useful model to study chromatin control of gene expression and suggests that disrupting the regulation of viral chromatin states can possibly be exploited as a novel antiviral therapeutic target. (asm.org)
  • Several viral gene products have been implicated in playing a role in generation of the euchromatin-like state of the viral genome ( 1 , 2 , 7 , 8 ), suggesting that HSV-1 encodes multiple activities to ensure that its genome remains competent for transcription despite the cellular response that attempts to silence foreign gene expression. (asm.org)
  • it remains unclear whether CAF-1 has a role in specific signaling cascades to promote gene expression during development. (biologists.org)
  • DNA replication can play a role in changing patterns of gene expression ( 1-3 ) and thus is a possible mechanism for disrupting chromatin states before their reprogramming and for the de novo establishment of those states. (sciencemag.org)
  • Chromatin-remodeling enzymes play essential roles in many biological processes, including gene expression, DNA replication and repair, and cell division. (pubmedcentralcanada.ca)
  • These phenotypes in Saccharomyces cerevisiae included the ability to undergo mating-type switching ( 1 ), increased mutation rates of mtDNA ( 2 ), and regulation of invertase gene expression in sugar metabolism ( 3 ). (pubmedcentralcanada.ca)
  • The TERMINAL FLOWER 1 (TFL1) gene of Arabidopsis serves a key function in the development of the shoot apical meristem (SAM). (go.jp)
  • Overexpression of Arabidopsis CycD3-1 affects leaf development, leading to curled leaves with an increased number of smaller cells and partial loss of cellu-lar organization ( Meijer and Murray, 2001 ). (plantcell.org)
  • This entry represents the C-terminal domain of CAF-1 subunit p150, which is part of the binding region to the p60 subunit [ PMID: 12697822 , PMID: 21209461 ]. (ebi.ac.uk)
  • Mutation of histone acetyltransferase (HAT) 1 and deacetylase (HDAC) enzymes is associated with a plethora of disease states in humans including cancers and developmental defects. (mcponline.org)
  • Our genetic analyses indicate that CAF-1 plays a role in both homologous recombination and nonhomologous end-joining pathways and that the function of CAF-1 during double-strand repair is distinct from that of another histone H3-H4 chaperone, anti-silencing function 1 ( ASF1 ). (genetics.org)
  • During MUG, chromatin is rapidly separated from the forming spindle, and both centrosomal and noncentrosomal spindle assembly pathways are active. (rupress.org)
  • This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. (springer.com)
  • To fully understand these repair processes, we have to focus on chromatin, the template upon which repair occurs. (genetics.org)
  • TIF1β is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. (biolegend.com)
  • Current studies reinforce the idea of intimate crosstalk between chromatin features and processes involving DNA transactions. (biomedcentral.com)
  • As a consequence, intimate connections exist between these three basic processes and chromatin structure and dynamics. (biomedcentral.com)
  • The crucial first step in this direction is a systematic study of factors that mediate this process. (yu.edu)
  • We have begun to analyze protein factors that mediate protamine deposition during spermatogenesis and their removal from DNA after fertilization. (yu.edu)