A highly vascularized extra-embryonic membrane, formed by the fusion of the CHORION and the ALLANTOIS. It is mostly found in BIRDS and REPTILES. It serves as a model for studying tumor or cell biology, such as angiogenesis and TISSUE TRANSPLANTATION.
An extra-embryonic membranous sac derived from the YOLK SAC of REPTILES; BIRDS; and MAMMALS. It lies between two other extra-embryonic membranes, the AMNION and the CHORION. The allantois serves to store urinary wastes and mediate exchange of gas and nutrients for the developing embryo.
The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.
A hard or leathery calciferous exterior covering of an egg.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.
Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The capability of producing eggs (OVA) from which young are hatched outside the body. While mostly referring to nonmammalian species, this does include MAMMALS of the order MONOTREMATA.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
A species of ORTHOPOXVIRUS causing infections in humans. No infections have been reported since 1977 and the virus is now believed to be virtually extinct.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Endothelial cells that line venous vessels of the UMBILICAL CORD.
A solid tumor consisting of a dense infiltration of MAST CELLS. It is generally benign.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A family of double-stranded DNA viruses infecting mammals (including humans), birds and insects. There are two subfamilies: CHORDOPOXVIRINAE, poxviruses of vertebrates, and ENTOMOPOXVIRINAE, poxviruses of insects.
The minute vessels that connect the arterioles and venules.
A genus of BIRDS in the family Phasianidae, order GALLIFORMES, containing the common European and other Old World QUAIL.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Virus diseases caused by the POXVIRIDAE.
A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
A cell line derived from cultured tumor cells.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Established cell cultures that have the potential to propagate indefinitely.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The development of the PLACENTA, a highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products between mother and FETUS. The process begins at FERTILIZATION, through the development of CYTOTROPHOBLASTS and SYNCYTIOTROPHOBLASTS, the formation of CHORIONIC VILLI, to the progressive increase in BLOOD VESSELS to support the growing fetus.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.
Transplantation between animals of different species.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Proteins prepared by recombinant DNA technology.
An enzyme that catalyzes the endonucleolytic cleavage of pancreatic ribonucleic acids to 3'-phosphomono- and oligonucleotides ending in cytidylic or uridylic acids with 2',3'-cyclic phosphate intermediates. EC 3.1.27.5.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A non-fibrillar collagen found in BASEMENT MEMBRANE. The C-terminal end of the alpha1 chain of collagen type XVIII contains the ENDOSTATIN peptide, which can be released by proteolytic cleavage.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Elements of limited time intervals, contributing to particular results or situations.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Adherence of cells to surfaces or to other cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A mature haploid female germ cell extruded from the OVARY at OVULATION.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN).
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The outer of the three germ layers of an embryo.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
The main trunk of the systemic arteries.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
Glycoproteins found on the membrane or surface of cells.
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
Signal molecules that are involved in the control of cell growth and differentiation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
Glycoproteins which have a very high polysaccharide content.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Transport proteins that carry specific substances in the blood or across cell membranes.
The rate dynamics in chemical or physical systems.
Cell membranes associated with synapses. Both presynaptic and postsynaptic membranes are included along with their integral or tightly associated specializations for the release or reception of transmitters.
Peptides composed of between two and twelve amino acids.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.

Inhibitory effects of 9-(4-thio-beta-D-ribo-pentofuranosyl)guanine on tumor growth and angiogenesis. (1/287)

BACKGROUND: To find a nucleoside with anti-angiogenic activity, we tried to screen an active compound from our nucleoside library. MATERIALS AND METHODS: The compound inhibiting the growth of human umbilical vein endothelial cell (HUVEC) induced by the conditioned medium of lung carcinoma cell line PC-9 was screened. The antitumor activity of the compound was evaluated against murine sarcoma S-180 implanted onto chick embryo chorioallantoic membrane (CAM). RESULTS: 9-(4-Thio-beta-D-ribo-pentofuranosyl)guanine (4'-thioguanosine) was found to be a potent inhibitor of the growth of HUVEC. The growth of S-180 implanted onto CAM was also inhibited by 4'-thioguanosine whereas the in vitro growth of S-180 was not inhibited. The administration of 4'-thioguanosine in mice caused unexpected side effect which suggested neurotoxicity. CONCLUSIONS: Antitumor effect of 4'-thioguanosine on S-180 was suggested to be due to inhibition of tumor angiogenesis. Because of toxicity of 4'-thioguanosine in mice, further development of the derivatives which have lower toxicity is required.  (+info)

Proangiogenic action of thyroid hormone is fibroblast growth factor-dependent and is initiated at the cell surface. (2/287)

The effects of thyroid hormone analogues on modulation of angiogenesis have been studied in the chick chorioallantoic membrane model. Generation of new blood vessels from existing vessels was increased 3-fold by either l-thyroxine (T4; 10(-7) mol/L) or 3,5,3'-triiodo-l-thyronine (10(-9) mol/L). T4-agarose reproduced the effects of T4, and tetraiodothyroacetic acid (tetrac) inhibited the effects of both T4 and T4-agarose. Tetrac itself was inactive and is known to block actions of T4 on signal transduction that are initiated at the plasma membrane. T4 and basic fibroblast growth factor (FGF2) were comparably effective as inducers of angiogenesis. Low concentrations of FGF2 combined with submaximal concentrations of T4 produced an additive angiogenic response. Anti-FGF2 inhibited the angiogenic effect of T4. The proangiogenic effects of T4 and FGF2 were blocked by PD 98059, a mitogen-activated protein kinase (MAPK) pathway inhibitor. Endothelial cells (ECV304) treated with T4 or FGF2 for 15 minutes demonstrated activation of MAPK, an effect inhibited by PD 98059 and the protein kinase C inhibitor CGP41251. Reverse transcription-polymerase chain reaction of RNA extracted from endothelial cells treated with T4 revealed increased abundance of FGF2 transcript at 6 to 48 hours, and after 72 hours, the medium of treated cells showed increased FGF2 content, an effect inhibited by PD 98059. Thus, thyroid hormone is shown to be a proangiogenic factor. This action, initiated at the plasma membrane, is MAPK dependent and mediated by FGF2.  (+info)

Effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenoviruses on antiangiogenesis. (3/287)

AIM: To investigate the inhibitory effects of endostatin-vascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization. METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane (CAM) model, rabbit inflammatory corneal neovascularization (CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo. RESULTS: Western blot showed that the molecular weight of fusion protein was about 41 kD after infection of ECV304, HepG2 and L929 cells with supernatant of AdhENDO-VEGI151. The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001), as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9+/-1849.9 mm(3)), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7+/-241.2 mm(3)) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75+/-3.31%) than in AdLacZ control (50.25+/-8.65%) (F=17.72, P=0.0056) with an inhibition rate of 39%. CONCLUSION: Fusion protein expressed by recombinant adenoviruses has a significant inhibitory effect on neovascularization.  (+info)

Regulation of neovascularization by human neutrophil peptides (alpha-defensins): a link between inflammation and angiogenesis. (4/287)

Angiogenesis, the growth of new blood vessels, is a complex biological process that is orchestrated by several growth factors and components of the extracellular matrix, including fibronectin (FN) and its receptor the integrin alpha5beta1. Angiogenesis is a critical part of inflammation and wound repair, but the mechanism by which vascular proliferation and migration is regulated by inflammatory cells is not completely understood. We have previously shown that human neutrophil peptides (HNPs), also known as alpha-defensins, which are secreted in high concentrations when neutrophils are activated, bind specifically to FN in the extracellular matrix and inhibit plasminogen activation. Therefore, we asked whether HNPs act as a link between inflammation and angiogenesis. Alpha5beta1-mediated endothelial cell adhesion and migration to FN, both under control conditions and under stimulation by vascular endothelial growth factor (VEGF), were inhibited specifically and in a dose-dependent manner by HNPs, whereas endothelial cell adhesion and migration to other components of the extracellular matrix, such as vitronectin, collagen, or fibrinogen/fibrin were not. Consistent with this finding, HNPs bound to and promoted the binding of fibronectin to alpha5beta1 integrin in arginine-glycine-aspartic acid (RGD)-independent manner. HNPs also completely inhibited VEGF-induced proliferation and induced apoptosis of endothelial cells in a dose-dependent manner. Moreover, HNPs inhibited capillary tube formation in three-dimensional fibrin-matrices as well as neovascularization in vivo in the chicken chorioallantoic membrane assay. Taken together, these data indicate that HNPs can regulate angiogenesis by affecting endothelial cell adhesion and migration in an FN-dependent manner as well as endothelial cell proliferation. These findings provide new insight into the role of inflammatory cells in angiogenesis and might provide a platform for developing a novel class of anti-angiogenesis drugs.  (+info)

Inhibiting MMP activity prevents the development of endometriosis in the chicken chorioallantoic membrane model. (5/287)

BACKGROUND: Matrix metalloproteinases (MMPs) are essential for extracellular matrix remodelling and may contribute to the development of endometriosis. Transplantation of endometrium onto the chicken chorioallantoic membrane (CAM) results in endometriosis-like lesion formation, a process that requires extensive tissue remodelling. We investigated the expression of a wide range of MMPs in menstrual endometrium, endometriosis-like lesions in CAMs, in peritoneal endometriosis and in endometriosis in the rectovaginal space, as well as the function of MMPs in early lesion formation in the CAM model. METHODS: Expression of MMPs was evaluated by immunohistochemistry and MMP function was studied in the CAM by inhibiting MMP activity during lesion formation. RESULTS: Nearly all MMPs were present in all tissues studied. No significant differences in the expression of a majority of MMPs were found in endometriosis-like lesions in CAMs when compared with human endometriosis. Inhibition of MMP-1, -2, -3, -7 and -13 activities significantly impaired endometriosis-like lesion formation in CAMs. CONCLUSIONS: The MMP expression profiles of experimentally induced endometriosis in CAMs and human endometriosis are similar. The prevention of endometriosis-like lesion formation in the CAM by inhibiting MMP activity strongly suggests that MMPs have a function in the early development of endometriotic lesions.  (+info)

Antiangiogenic activity of semisynthetic biotechnological heparins: low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists. (6/287)

OBJECTIVE: Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives. METHODS AND RESULTS: Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and alpha(v)beta3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/alpha(v)beta3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane. CONCLUSIONS: LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/alpha(v)beta3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.  (+info)

X-rays affect the expression of genes involved in angiogenesis. (7/287)

BACKGROUND: We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that are important regulators of angiogenesis: vascular endothelial growth factor (VEGF), heparin affin regulatory peptide (HARP) and inducible nitric oxide synthase (iNOS). MATERIALS AND METHODS: An area of 1 cm2 of the CAM, restricted by a plastic ring was irradiated at room temperature. The expression of the genes was studied using RT-PCR and the amounts of the mRNAs were quantified using image analysis of the corresponding agarose gels of the RT-PCR products. RESULTS: VEGF mRNA was decreased 6 h after irradiation. However, at later time points, VEGF expression was significantly increased compared with the nonirradiated tissue. Similarly, X-rays down-regulated both HARP and iNOS expression 6 h after irradiation and the effect was reversed at later time points, similarly to the effect of X-rays on VEGF. CONCLUSION: These data support the notion that X-rays increase the expression of genes that favor angiogenesis.  (+info)

Angiogenic response induced by acellular aortic matrix in vivo. (8/287)

In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a well-known angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-beta1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-beta1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-beta1 in the acellular matrices.  (+info)

TY - JOUR. T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. AU - Mangieri, Domenica. AU - Nico, Beatrice. AU - Benagiano, Vincenzo. AU - De Giorgis, Michela. AU - Vacca, Angelo. AU - Ribatti, Domenico. PY - 2008/6. Y1 - 2008/6. N2 - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve ...
TY - JOUR. T1 - Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2. AU - Nico, Beatrice. AU - Ennas, Maria Grazia. AU - Crivellato, Enrico. AU - Frontino, Annalisa. AU - Mangieri, Domenica. AU - De Giorgis, Michela. AU - Roncali, Luisa. AU - Ribatti, Domenico. PY - 2004/7. Y1 - 2004/7. N2 - Pericytes stabilize vessel wall, participate in the regulation of blood flow microcirculation and influence endothelial proliferation, survival, migration and maturation. Pericytes are commonly identified by molecular markers, such as desmin. The aim of this study was to analyze the pattern of distribution of desmin in the chick embryo chorioallantoic membrane (CAM) vasculature in normal condition and in response to an angiogenic cytokine, such as fibroblast growth factor-2 (FGF-2) by using conventional immunohistochemistry and confocal laser scanning microscopy. Results indicate that, in normal condition, the desmin-positive pericytes are located ...
The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes, such as birds and reptiles. It is formed by the fusion of the mesodermal layers of two developmental structures: the allantois and the chorion.[1] In mammals, this structure forms the placenta. Three different layers compose the chorioallantoic membrane; these are called the chorionic epithelium, the mesenchyme and the allantoic epithelium. Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen, to developing embryos.[1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell.[1] Chorioallantoic membranes from developing ...
The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay
Angiogenic potential of the cerebrospinal fluid (CSF) of patients with high-grade gliomas measured with the chick embryo chorioallantoic membrane assay (CAM) ...
537 The expression of matrix metalloproteinases (MMPs) plays crucial roles in cancer progression and metastasis. This study is to clarify whether MMP associates with radiation-accelerated pulmonary metastasis of Lewis lung carcinoma (LLC-LM) in C57BL/6 mouse model. By using in vitro matrigel-coated Boyden chamber invasion assay, non-cytotoxic dose of radiation (7.5 Gy) significantly enhanced LLC-LM cell invasiveness. Irradiated LLC-LM cells showed the increased intravasation capability in the chicken chorioallantoic membrane assay. To test MMP family protein gene expression in irradiated LLC-LM cells, MMP-9 gene expression was significantly enhanced in both transcriptional and translational levels, so was the enzyme activity in the culture supernatant. The use of anti-sense MMP-9 oligonucleotides significantly inhibited the in vitro radiation-enhanced invasiveness. With 1 x 106 MMP-9 RNAi stably transfected LLC-LM cells injected subcutaneously in the right thighs of C57BL/6 mice and irradiated ...
Leptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. Images where taken after every 24 h of incubation and analysed with Angioguant software. The treated area was observed under microscope and
The overall objectives of this thesis were to develop a short-term culture system and to examine the effects of vitrification and short-term culture on the viability of fresh and vitrified bovine ovarian tissue and the follicles within. The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. Bovine ovaries were retrieved from a local abattoir and cortical pieces (1-2mm3) were randomly assigned to one of the following groups; control (fixed immediately), CAM or in in vitro culture. Ovarian tissue fragments from both groups were removed on D1, D3 and D5 of culture, fixed, sectioned (5μm) and stained with H&E. The numbers of healthy and ...
Shark (Sinica cetorhinus maximum) cartilage was extracted in 1 mol/L Gu-HCl guanidine. Two purified active proteins with apparent molecular weights of 15.2x103 Da and 8.0×103 Da (designated as Sp15 and Sp8, respectively) were obtained through ultrafiltration and Superdex 75 chromatography. The activities of the samples were studied in terms of their potential inhibition of vascular endothelial cell growth in vitro, of angiogenesis both in rabbit cornea and chick embryo chorioallantoic membrane (CAM) assay models in vivo, and of growth of transplanted S180 sarcoma in mice in vivo. The results showed that Sp15 expressed a typical lysozymatic activity up to 223,000 U/mg and its N-terminus was highly homologous to lysozymes of various mammalian origins. Sp15 exhibited a strong anti-angiogenic activity only in vitro, whereas Sp8 shared this effect both in vitro and in vivo. Both Sp15 and Sp8 provided an effective anti-tumor activity in mice bearing transplanted S180 sarcoma. These results suggest that Sp15
The aims of this work are to study the antitumor effect of the adeno-associated virus on the xenografted tumors of chick embryo chorioallantoic membrane and predict potential genes and biological pathways which are associated with renal cell carcinoma. The adeno-associated virus NT4-TAT-6 × His-VHLbeta was constructed and identified. Then, chick embryos with xenografted tumor were divided into three groups and respectively inoculated with rAAV/NT4-TAT-6 × His-VHLbeta (group A), empty virus (group B), and phosphate-buffered saline (group C, the control subject). Antitumor effect in each group was investigated by means of immunofluorescence observation. Genes interacted with von Hippel-Lindau were screened by Search Tool for the Retrieval of Interacting Genes/Proteins database, while pathway analysis were performed based on Kyoto Encyclopedia of Genes and Genomes. The growth of xenografted tumors inoculated with recombinant adeno-associated virus was slower than the control subjects. The tumor ...
Introduction: The membrane-bound metalloproteinase aminopeptidase N (APN) is involved in several different cellular processes associated with the malignant phenotype. Furthermore APN has also been reported to have a functional role in tumor angiogenesis and there appear to be a strong correlation between the expression of APN and the invasive capacity of a numerous tumor cell types. The melphalan-derived prodrug melphalan-flufenamide (previously designated J1) can be activated by APN suggesting possible anti-angiogenic properties.. Experimental: A series of experiments were performed to evaluate the effects of melphalan-flufenamide on angiogenesis; cytotoxicity on bovine endothelial cells, the TCS Cellworks AngioKit model with human endothelial cells co-cultured with fibroblasts, the chicken embryo chorioallantoic membrane assay, and finally in vivo in mice using the Cultrex DIVAA angio-reactor assay.. Results: Melphalan-flufenamide displayed high cytotoxic activity against endothelial cells, ...
When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. The number of foci is positively correlated with the number of cells in the inoculum. The phenomenon is known to be an expression of an immunological reaction of the grafted cells against the host. F. M. Burnet and his colleagues have suggested that each focus results from the immunological activity of one and only one cell. If this presumption were confirmed the CAM system would gain in usefulness, in particular for the isolation of clones of immunologically competent cells. In the present work this single-hit hypothesis has been subjected to a specific test of its biometrical consequences. On the single-hit model the response should be proportional to the dose. Equivalently, on the logarithmic scale of measurement adopted in these experiments, the fitted dose-response line should show a ...
The authors studied the specific tumor antigen in Crockers sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing...
One of the major problems in assessing neovascularization in mammalian experimental animal models is the immunologic response of the host to stimuli from nonautologous species. Hence, crude bovine vitreous and retinal extracts may produce a complex immune reaction when tested in the rabbit. To circumvent this problem, the chicken chorioallantoic membrane (CAM) assay is most appropriate. In this study the CAM assay for angiogenesis has been modified to study antiangiogenic substances. The modified assay is described in detail and used to demonstrate for the first time the inhibition by adult bovine vitreous of neovascularization induced by extracts of adult bovine retina. In addition to vitreous, three common glycosaminoglycans (keratan sulfate, chondroitin sulfate C, and hyaluronic acid) were assayed for antiangiogenic activity. The results indicate that vitreous inhibition of retinal extract-induced neovascularization is dose dependent, while the sulfated glycosaminoglycans tested had no ...
Keywords: Individual neonatal foreskin stromal cells (hNSSCs), Girl embryo chorioallantoic membrane layer (Camera), Individual umbilical line of thinking endothelial cells (HUVEC), Micro mass, Girl femur, Ex girlfriend vivo, Organotypic lifestyle 1.?Launch Control cell therapies keep the guarantee for healing a large amount of age-related degenerative illnesses including nonunion bone injuries, brittle bones, and arthritis. Nevertheless, suitable make use of of cells in the medical clinic needs the capability of examining the cells in an suitable model predictive for in vivo phenotype (Wei et al., 2013). Typically, ex girlfriend 52549-17-4 supplier vivo cell civilizations have got been used to check the difference potential of control cells. While useful, culturing the cells in 2-Chemical environment will not really reveal the in vivo circumstance. On the various other hands, ectopic transplantation of control cells in can offer biologically relevant details but these assays are costly vivo, ...
The long‐term survival and successful integration of implants for tissue replacement and regeneration highly depends upon the fast ingrowth of blood vessels from the surrounding tissues
Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of ...
MT Pinto joined Ipatimup as an Investigator Ciência 2008 (2009) of the Cancer Genetics Group and was mainly dedicated to the study of cancer related features using the CAM model. After setting up the lab and implementing several CAM assays, she established the In vivo CAM assays service at Ipatimup (2012) and founded the spin-off Expertus, CAM assays (2013-2015). MT Pinto graduated in Biochemistry (FCUP; 1999) and both MSc (FMUL; 2001) and PhD (FMUP; 2008) theses concerned the study of the endogenous pain control system in acute and chronic pain states and involved extensive use of animal models. MT Pinto has published 26 papers in international peer reviewed journals (Q1) with an H index of 12 (ResearcherID: J-2519-2013). She is co-author of two patents. ...
Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis. Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent. Results showed that TF-SB
Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Figure 6: |i|Viola tricolor|/i| Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane
TY - JOUR. T1 - Retinoic acid-induced glandular metaplasia in mouse skin is linked to the dermal expression of retinoic acid receptor β mRNA. AU - Viallet, Jean P.. AU - Ruberte, Esther. AU - du Manoir, Stanislas. AU - Krust, Andrée. AU - Zelent, Arthur. AU - Dhouailly, Danielle. PY - 1991/4. Y1 - 1991/4. N2 - The distribution of transcripts of nuclear (RARα, RARβ, and RARγ) and cytosolic (CRABP) retinoic acid receptors was analyzed in 13.5-day mouse embryo upper-lip skin, cultured in vitro for 48 hr with or without added retinoic acid. The results show a significant up-regulation of the transcription of the RARβ gene concomitant with the initiation of an alteration of hair vibrissae follicle development, leading, after transfer for 8 days to the chick embryo chorioallantoic membrane, to an exocrine-type gland morphogenesis.. AB - The distribution of transcripts of nuclear (RARα, RARβ, and RARγ) and cytosolic (CRABP) retinoic acid receptors was analyzed in 13.5-day mouse embryo ...
TY - JOUR. T1 - Hydrogen sulphide induces angiogenesis. AU - Pyriochou, Anastasia. AU - Marazioti, Antonia. AU - Szabo, Csaba. AU - Papapetropoulos, Andreas. PY - 2009. Y1 - 2009. N2 - Hydrogen sulphide (H2S) is emerging as an important signalling molecule in the cardiovascular and neuronal systems. Although H2S is known to relax vascular smooth muscle leading to a reduction in blood pressure, the role of this gaseous mediator in other aspects of the biology of vascular cells remains largely unexplored. In order to study the effects of H2S on neovascularisation, we have utilised an aqueous formulation of H2S. Incubation of endothelial cells (EC) with H2S enhanced their angiogenic potential as it promoted cell growth, migration and capillary morphogenesis on matrigel. In line with the in vitro observations, treatment of chicken chorioallantoic membranes (CAM) with H2S increased vascular length. Morever, exposure of EC to H2S resulted in increased phosphorylation of kinases linked to angiogenic ...
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number of human diseases. In vivo neovascularization assays that reflect physiological and pathological formation of neovessels are important in this effort. In this report we present an assay where the effects of activators and inhibitors of angiogenesis can be quantitatively and qualitatively measured. A provisional matrix composed of collagen I and fibrin was formed in a plastic cylinder and implanted onto the chick chorioallantoic membrane. A nylon mesh separated the implanted matrix from the underlying tissue to distinguish new from pre-existing vessels. Vascularization of the matrix in response to fibroblast growth factor-2 or platelet-derived growth factor-BB was scored in a double-blinded manner, or vessel density was measured using a semi-automated image analysis procedure. Thalidomide, fumagillin, U0126 and TGFβ inhibited neovessel growth while hydrocortisone exerted a negative and ...
ABSTRACT: 15 K is 1,2, 3-triazolyl ester of ketorolac, an old pain-killer, that blocks PAK1 by its R-form and inhibits COX-2 by its S-form. Mainly due to a robust increase in cell-permeability, 15K is over 500 times more potent than ketorolac in both anti-cancer and anti-PAK1 activities in cell culture with IC50 around 24 nM. However, 15K has no anti-AKT activity. Angiogenesis requires at least the kinase PAK1, and perhaps the kinase AKT as well, and is essential for a robust growth of solid tumors. Thus, in this study, we examined the potential antiangiogenic activity of 15K both in ovo and cell culture, prior to its in vivo (xenograft) anti-cancer activity test. The IC50 of 15K against the embryonic angiogenesis in ovo in CAM (chorioallantoic membrane) assay is around 1 nmol/egg. Surprizingly, however, 15K failed to inhibit the tube formation of HUVECs (human umbilical vein endothelial cells) in cell culture even at high as 150 μM. In an attempt to solve this mystery, we tested both in ovo as ...
Interferons were first described in 1957 by Alick Isaacs and Jean Lindenmann at the National Institute for Medical Research in London;[42][43][44] the discovery was a result of their studies of viral interference. Viral interference refers to the inhibition of virus growth caused by previous exposure of cells to an active or a heat-inactivated virus. Isaacs and Lindenmann were working with a system that involved the inhibition of the growth of live influenza virus in chicken embryo chorioallantoic membranes by heat-inactivated influenza virus. Their experiments revealed that this interference was mediated by a protein released by cells in the heat-inactivated influenza virus-treated membranes. They published their results in 1957 naming the antiviral factor they had discovered interferon.[43] The findings of Isaacs and Lindenmann have been widely confirmed and corroborated in the literature.[45]. Furthermore, others may have made observations on interferons before the 1957 publication of Isaacs ...
Glaucium flavum (G. flavum) is a plant from the Papaveraceae family native to Algeria where it is used in local traditional medicine to treat warts. G. flavum root crude alkaloid extract inhibited breast cancer cell proliferation and induced G2/M phase cycle arrest and apoptosis without affecting normal cells, which is a highly awaited feature of potential anti-cancer agents. G. flavum significantly reduced growth and vascularization of human glioma tumors on chicken chorioallantoic membrane (CAM) in vivo. The chromatographic profile of the dichloromethane extract of G. flavum root showed the presence of different constituents including the isoquinoline alkaloid protopine, as the major compound. We report for the first time that G. flavum extract may represent a new promising agent for cancer chemotherapy.
Anticancer Effect of COX-2 Inhibitor DuP-697 Alone and in Combination with Tyrosine Kinase Inhibitor (E7080) on Colon Cancer Cell Lines Colon cancer;DuP-697;COX-2 inhibition;tyrosine kinase inhibitor; Colorectal cancer remains one of the most common types of cancer and a leading cause of cancer death worldwide. In this study, we aimed to investigate effects of DuP-697, an irreversible selective inhibitor of COX-2 on colorectal cancer cells alone and in combination with a promising new multi-targeted kinase inhibitor E7080. The HT29 colorectal cancer cell line was used. Real time cell analysis (xCELLigence system) was conducted to determine effects on colorectal cell proliferation, angiogenesis was assessed with a chorioallantoic membrane model and apoptosis was determined with annexin V staining. We found that DuP-697 alone exerted antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. For the antiproliferative effect the half maximum inhibition concentration ($IC_{50
TY - JOUR. T1 - Wogonin inhibits IL-6-induced angiogenesis via down-regulation of VEGF and VEGFR-1, not VEGFR-2. AU - Lin, Chiu Mei. AU - Chang, Hang. AU - Chen, Yeh Hsu. AU - Wu, I. Hsing. AU - Chiu, Jen Hwey. PY - 2006/11. Y1 - 2006/11. N2 - So far, no antiangiogenic activity of wogonin, a flavonoid, on human umbilical vein endothelial cells (HUVECs) has been demonstrated. The aim of this study was to investigate the effects of wogonin on IL-6-induced angiogenesis in HUVEC cultures and chorioallantoic membrane (CAM) neovascularization. The in vivo CAM model was applied to evaluate the percentage of new vessel formations, followed by measurement of endothelial migration and tube formation in HUVEC cultures. The results revealed that wogonin (10-8-10-5M) concentration-dependently inhibited IL-6-induced angiogenesis. The signaling pathway was through down-regulation of the autocrine loop of VEGF and VEGFR-1, but not of VEGFR-2. Furthermore, the regulating loop of the IL-6 receptor complex was ...
Authors: Riordan NH, Meng X, Riordan HD.. ABSTRACT: Recruitment of new blood vessels plays a crucial role in tumor survival and growth. Several agents that act as angiogenesis inhibitors are currently being investigated as anti-tumor agents. Proteoglycan extract (PGM) was tested for anti-angiogenic, immunostimulatory, and anti-neoplastic activity. PGM is a non-toxic extract of the ubiquitous plant, Convolvulus arvensis. In the chicken egg chorioallantoic membrane assay PGM inhibited new blood vessel growth in a dose-dependent manner. Results were 18, 55, and 73% inhibition at concentrations of 50, 100, and 200 mcg, respectively. PGM significantly inhibited tumor growth in the mouse fibrosarcoma (S-180 Kun Ming 3-4 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days), and mouse Lewis lung carcinoma (C57, 6 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days) models. Inhibition (54-77% inhibition by weight compared to controls, up ...
A swept source based Fourier domain optical Doppler tomography (FDODT) system was developed. The technique is based on a phase-resolved method where phase information was retrieved from the reconstructed complex fringe signals. The aliasing effects and artifacts caused by lateral scanning and sample movement were removed with a signal processing technique. The standard deviation of the phase shift of the system was reduced from 49 to 1.8 degrees with the signal processing method employed. Structural, Doppler and Doppler variance images of fluid flow through glass channels were quantified, and blood flow through vessels of chick chorioallantoic membrane (CAM) was demonstrated in vivo.. ©2005 Optical Society of America. Full Article , PDF Article ...
J:31267 Spindle A, Sturm KS, Flannery M, Meneses JJ, Wu K, Pedersen RA, Defective chorioallantoic fusion in mid-gestation lethality of parthenogenonetetraploid chimeras. Dev Biol. 1996 Feb 1;173(2):447-58 ...
Rigorous and systematic pre-clinical studies are necessary and essential to establish the efficacy and safety of Oriental herbs and formulas in order to transform traditional herbal practices into evidence-based medicine. Here we evaluated the anti-cancer activities of the ethanol extract of Ka-mi-kae-kyuk-tang (KMKKT), a formula of ten Oriental herbs, with a battery of in vitro and in vivo mechanism-based biomarkers involving angiogenesis, apoptosis and metastasis. The results show that KMKKT suppressed the vascular endothelial responses by inhibiting basic fibroblast growth factor (bFGF)-induced ERK1/2 phosphorylation, cell migration as well as tube formation in the human umbilical vein endothelial cell model, and decreased the hypoxia-induced HIF1α and vascular epithelial growth factor (VEGF) expression in the mouse Lewis lung carcinoma (LLC) cells in vitro, and inhibited the bFGF-induced angiogenesis in chick chorioallantoic membrane model, and in the Matrigel plugs in mice. Intraperitoneal ...
ODUOR PROFOKELLODOMINIC. NJOGU, A., OWITI, G.E.O. PERSSON, E. & ODUOR- OKELO (2006) Ultrastructure of the chorioallantoic placenta and chorionic vesicle of the lesser bush baby(Galago senegalensis). Placenta 27: 771-779.. In: MSc. Thesis, University of Nairobi. Kisipan, M.L.; 2006 ...
Definition of chorioallantoic placenta. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Shikonin has anticancer activity, but it has not yet been applied into clinical use. In the present study, shikonin was prepared using liposomes. We aimed to examine several aspects of sh-L (shikonin-containing liposomes): preparation, angiogenic suppression and cellular uptake through self-fluorescence. Sh-L were prepared using soybean phospholipid and cholesterol to form the membrane and shikonin was encapsulated into the phospholipid membrane. Three liposomes were prepared with shikonin. They had red fluorescence and were analysed using a flow cytometer. Angiogenic suppression of sh-L was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide], Transwell tests, chick CAM (chorioallantoic membrane) and Matrigel™ plug assay. MTT assay showed the median IC50 (inhibitory concentrations) as follows: shikonin, sh-L1 and sh-L2 were 4.99±0.23, 5.81±0.57 and 7.17±0.69 μM, respectively. The inhibition rates of migration were 53.58±7.05, 46.56±4.36 and ...
Studies on further attenuated live measles vaccine. I. Adaptation of measles virus to the chorioallantoic membrane of chick embryo and clinical tests on the str
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Background. Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive solid cancers with a 5-year survival rate approaching a dismal 5%. New therapeutic targets need to be identified in order to increase survival rates.. HOX genes are a family of 39 transcription factors involved in embryonic development and which have been found to be dys-regulated in PDAC. HOX gene function can be antagonised by HXR9, a peptide that competitively inhibits binding of HOX proteins to their co-factor PBX. We established that the CAM model recapitulates human PDAC, including desmoplasia, and is highly suitable for the evaluation of HXR9 in vivo.. Experimental procedure. Four PDAC-derived cell lines (AsPc-1, BxPc-3, PaCa-3 and PancTu1) were treated in vitro with HXR9. Ic50s were determined using both MTS and LDH assays. FACS analysis was used to determine mechanisms of cell death. Cell lines were subsequently implanted in the CAM using Marigel™ on embryonic day (EDD) 10. Tumour growth curves were ...
If you dont agree with the NSA surveillance, but youre not concerned because it does not personally impact you, thats a point worth exploring.
In chick chorioallantoic membrane (CAM) assays, injection of cilengitide into CAM vasculature prevented the growth of implanted U87MG glioma and DAOY medulloblastoma tumor fragments compared to the inactive peptide EMD135981. The inhibition of tumor growth was attributed to the anti-angiogenic activity of cilengitide as membrane vascularity surrounding the tumor graft was greatly decreased by cilengitide treatment as compared to the inactive control peptide [130]. In animal models, cilengitide, as a single agent effectively inhibited the growth and proliferation of orthotopically implanted malignant glioma and medulloblastoma cell lines in nude mice. Daily treatment of established xenografts with 100-200 µg cilengitide significantly delayed tumor growth and prolonged survival compared to controls. The delayed tumor growth was accompanied by increases in CD31 and tumor doubling times, elevated apoptotic index and decreases in proliferative index and tumor vessel number and density [130]. ...
Abstract: Often the fields of tissue engineering and cancer research are studied in isolation, inhibiting cross-talk and collaboration between cancer researchers and tissue engineers. This work has aimed at bridging these fields in a way that provides insight into the clinical problems of breast cancer and bone metastasis. The research discussed herein focuses on how host cells contribute to the g... read morerowth and progression of breast cancer during bone metastasis. Preliminary studies used the chick CAM, chorioallantoic membrane, to investigate angiogenesis and bone metastasis (Chapter 2). Then mouse models and tissue-engineered bone were utilized to study mesenchymal stem cell (MSC) tumor homing and the effects of MSCs on cancer progression (Chapter 3). Results suggested that tumor-derived TGF-beta1 (transforming growth factor-beta1) is likely to play a large role in attracting MSCs to breast tumors in patients. MSC-derived IL-17B (interleukin 17B) was found to stimulate breast cancer ...
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The NSA has done one hell-ave job in China and the rest of Asia. This is not talked about much, the exact number is not known, but it has been stated that up to 100,000 business in China are owned by Tawian/NSA.. Almost all major U.S. companies have NSA members on the Boards of Directors. Most committees that report to congress also have members. Most presidents have also been members of the NSA. We know this because most presidents have been on the Media committee that controls the Media data that can be harmful to the public. I am not saying this is bad at all. Many of the NSA members are specialist in their field of education. The idea seems to be that the NSA uses top professors and people to help make decisions that effect the U.S. and the world. They work for our major colleges and universities. Working for the NSA is task driven. Finish the operation and wait for the next call. It is not like a full time job. Some companies that are NSA owned and operated are managed so well that the ...
When online you basically keep broadcasting, here is where I am, send me this data, information that is meant to be available to the server/servers you are connected to. They generally just give out where your from, what your network connection is like, what browser you use, port number, internet protocol etc. This information is meant to be used for running FTP and HTTP operations making ISP doorways where they who goes in and who goes out. Now with the NARUS semantic network scanning that the NSA is supposed to be using they not only know who goes out and who goes in they also know where their going, what theyre talking about and whom theyre talking too! The NSA could do this before too mind you, but this is the first time we know that they are doing this en-masse. Plus this is data directly sourced form the ISP meaning that even those connections routed through the AT&T server would go the NSA spybox ...
As new details emerge on NSA leaker Edward Snowden and his girlfriend, MSNBCs Lawrence ODonnell debates the NSA program with MSNBCs Chris Hayes and Alex Wagner.
Jewel v. NSA, EFFs landmark case challenging NSAs mass spying moved forward in 2019, setting up a crucial decision for the Ninth Circuit in 2020. Weve pursued this case for over a decade because we believe that mass surveillance, like all general search and seizure schemes, is both illegal and...
The planned NSA data center facility in Camp Williams in Utah will span 1 million square feet, with about 100,000 square feet dedicated to raised floor data center space.
New NSA revelations, a court strikes down the FCCs net neutrality rules, and the other tech policy stories you need to read today.
One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted ... on the CAM membrane of a chicken embryo outside the organism (chicken).[citation needed] Play media Animal testing In situ In ...
Wimsatt, W. A. Enders A. C.; Mossman, H. W. (Oct 1973). "A reexamination of the chorioallantoic placental membrane of a shrew, ... doi:10.1095/biolreprod12.1.1. Wimsatt, W. A. (Aug 1974). "Morphogenesis of the fetal membranes and placenta of the black bear, ... Wimsatt, W. A. (1954). "The fetal membranes and placentation of the tropical American vampire bat Desmodus rotundus murinus, ... Wimsatt, W. A. (Jan 1949). "Cytochemical observations on the fetal membranes and placenta of the bat, Myotis lucifugus ...
Definitive laboratory identification of Variola virus involved growing the virus on chorioallantoic membrane (part of a chicken ... By days 12-15, the first visible lesions - small reddish spots called enanthem - appeared on mucous membranes of the mouth, ... The viral envelope is made of modified Golgi membranes containing viral-specific polypeptides, including hemagglutinin.[42] ... A rash developed on the skin 24 to 48 hours after lesions on the mucous membranes appeared. Typically the macules first ...
Hen's egg test on chorioallantoic membrane. I. *Iron poisoning. L. *LD50. *Lead poisoning ...
The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos. Science. 1931;74(1919):371-372. ... either the outer membrane surrounding an infected host cell or internal membranes such as nuclear membrane or endoplasmic ... This membrane is studded with proteins coded for by the viral genome and host genome; the lipid membrane itself and any ... Release - Viruses can be released from the host cell by lysis, a process that kills the cell by bursting its membrane and cell ...
Goodpasture EW, Woodruff AM, Buddingh GJ (1931). "The cultivation of vaccine and other viruses in the chorioallantoic membrane ... "The growth in vitro of vaccinia virus in chick embryo chorio-allantoic membrane, minced embryo and cell suspensions". The ...
... chorioallantoic membrane - choriocarcinoma - choroid plexus tumor - CHPP - chronic granulocytic leukemia - chronic idiopathic ... synovial membrane - synovial sarcoma - synthetic protegrin analog - synthetic retinoid - systemic chemotherapy - systemic ... mesenteric membrane - mesna - mesonephroma - mesothelioma - metaplasia - metaplastic carcinoma - metastasectomy - metastasis - ...
Delovanje so preizkušali in vitro na podganji aorti ter in vivo na membrani zarodka piščanca (chorioallantoic membrane - CAM). ...
The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of ... with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases ... Ribatti D (2008). "Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis". Int Rev Cell Mol Biol. 270: ... Chorioallantoic membranes from developing chicken eggs are routinely used in biological and biomedical research to investigate ...
... with a system that involved the inhibition of the growth of live influenza virus in chicken embryo chorioallantoic membranes by ... such as membrane bound Toll like receptors or the cytoplasmic receptors RIG-I or MDA5, can trigger release of IFNs. Toll Like ... that this interference was mediated by a protein released by cells in the heat-inactivated influenza virus-treated membranes. ...
... the chorion and the allantois fuse together to form the chorioallantoic membrane. Together these form a double membrane, which ... The chorion is the outermost fetal membrane around the embryo in mammals, birds and reptiles. It develops from an outer fold on ... In reptiles, birds, and monotremes, the chorion is one of the four extraembryonic membranes that make up the amniotic egg that ... It is formed by extraembryonic mesoderm and the two layers of trophoblast that surround the embryo and other membranes. The ...
Chloroplast membrane. *Cholesterol depletion. *Cholinergic receptor, nicotinic, alpha 1. *Chorioallantoic membrane. *Christian ...
... the chorion and the allantois fuse together to form the chorioallantoic membrane. Together these form a double membrane, which ... The chorion is the outermost fetal membrane around the embryo in mammals, birds and reptiles (amniotes). It develops from an ... In reptiles, birds, and monotremes, the chorion is one of the four extraembryonic membranes that make up the amniotic egg that ... It is formed by extraembryonic mesoderm and the two layers of trophoblast that surround the embryo and other membranes. The ...
The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of ... with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases ... Ribatti D (2008). "Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis". Int Rev Cell Mol Biol. 270: ... Chorioallantoic membranes from developing chicken eggs are routinely used in biological and biomedical research to investigate ...
... sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the ... Specific antigens from tumors after prolonged cultivation on the chorioallantoic membrane of the chick embryo. ... sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the ...
Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM ... Figure 2. (a) Day 3 chick embryo; (b) Ovarian cancer cells and matrigel graft on the chick chorioallantoic membrane (CAM) on ... Figure 2. (a) Day 3 chick embryo; (b) Ovarian cancer cells and matrigel graft on the chick chorioallantoic membrane (CAM) on ... Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian ...
A quantitative study of the chorioallantoic membrane reaction in the chick embryo. L. W. Coppleson, D. Michie, Sir Peter ... When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing ... A quantitative study of the chorioallantoic membrane reaction in the chick embryo ... A quantitative study of the chorioallantoic membrane reaction in the chick embryo. ...
Angiogenesis within Stem Cell-Seeded Silk Scaffolds Cultured on the Chorioallantoic Membrane and Visualized by 3D Imaging. ... It includes seeding of stem cells into silk fibroin scaffolds, angiogenesis assay on the chorioallantoic membrane (CAM) of ...
We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than ... or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. Follicles from control and vitrified tissue were ... subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. ...
Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h ... In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane ...
... its chorioallantoic membrane (CAM). The research facility provides access to alternative/additional in vivo tools to evaluate ...
Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane ... Effect of ethyl acetate (EtOAc) fraction of V. tricolor on the number and diameter of vessels in chorioallantoic membrane (CAM ... Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane. ... of the ethyl acetate fraction was injected into the chorioallantoic sac. 1, 2, and 3 are representative of heart, artery, and ...
... isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay - Volume 93 Issue 3 - Serafim Kiriakidis ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay. * ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay ...
A novel angiogenesis model for screening anti-angiogenic compounds: the chorioallantoic membrane/feather bud assay.. [Haiming ... novel and rapid method to evaluate the anti-angiogenic activity of new agents consisting of a combined chorioallantoic membrane ...
Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C ... fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI- ... fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI- ... Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is ...
The chorio-allantoic membrane of the developing chick was successfully inoculated with a seven year old culture of Histoplasma ... Histoplasma Capsulatum: Its Cultivation on the Chorio-Allantoic Membrane of the Developing Chick and Resultant Lesions 1 * ... 1. The chorio-allantoic membrane of the developing chick was successfully inoculated with a seven year old culture of ...
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Conclusions: The chorioallantoic membrane culture system can be used to sustain the growth and development of isolated chick ... Methods: We have explanted the lung buds of developing day 4 chick or quail embryos onto the chorioallantoic membranes of host ... Results: We have demonstrated that developing lung buds explanted to chorioallantoic membranes continue to develop further, in ... Ex Vivo Culture of Lung Buds on the Chorioallantoic Membrane of the Avian Embryo ...
The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis - Author: Ribatti, Domenico - Price: 109, ... 3. Chorioallantoic Membrane in the Study of Tumor Angiogenesis. Domenico Ribatti. 4. Chorioallantoic Membrane in the Study of ... 1. Chorioallantoic Membrane Vasculature. Domenico Ribatti. 2. Chorioallantoic Membrane in the Study of Angiogenesis, ... The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis. 109,95€ ...
Angiogenesis and Diabetes: Different Responses to Pro-Angiogenic Factors in the Chorioallantoic Membrane Assay ...
This protocol describes three methods on how to obtain and use 5 to 8-day old chicken embryos and their chorioallantoic ... Use a scalpel to remove one of the two membranes from the holder with acoustic membrane.. ​NOTE: To remove the membrane, cut ... Schmitd, L. B., Liu, M., Scanlon, C. S., Banerjee, R., Silva, N. J. D. The Chick Chorioallantoic Membrane In vivo Model to ... The chorioallantoic membrane (CAM), embryo body (EB), anterior and posterior vitelline veins (*), and appropriate sites for ...
Epub ahead of print] Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein ... Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal ... Fifteen Influenza A virus reference subtypes, H1 to H15, infected chicken embryonated chorioallantoic membranes (CAM) fixed by ... Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal ...
Evaluation of pharmacological effect of Teucrium stocksianum extract on angiogenesis using chorioallantoic membrane assay * ... The present study was aimed to evaluate the effect of Teucrium stocksianum on angiogenesis by using chorioallantoic membrane ( ... "Evaluation of Pharmacological Effect of Teucrium Stocksianum Extract on Angiogenesis Using Chorioallantoic Membrane Assay". ...
The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform ... The chick embryo chorioallantoic membrane (CAM) model is well-known in human medicine, first described by Rous and Murphy [8]. ... The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform ... Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian ...
The in vivo test on the chorioallantoic membrane of the fertilized hens egg (CAM assay) is a current method to determine ... The in vivo test on the chorioallantoic membrane of the fertilized hens egg (CAM assay) is a current method to determine ... Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) assay ... was tested for its effect in the chorioallantoic membrane (CAM) assay. The essential oil composition was revealed by means of ...
The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane. It serves as a gas exchange surface through the ... Capillaries in the Chicken Chorioallantoic Membrane. Last Updated on Thu, 09 Oct 2014 , Endothelial Cells ... At day 14, the capillary plexus is located at the surface of the ectoderm adjacent to the shell membrane. The blood circulation ... Between days 9 and 13 the arteriolar endothelium displays more extensive junctional complexes with multiple membrane contact ...
Results for Chick chorioallantoic membrane. Publications & Outputs. *. Triethyl orthoformate covalently cross-linked chitosan-( ...
Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis.: Since their ... Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis.. Authors * Deryugina, ... The ability of the chick embryos chorioallantoic membrane (CAM) to efficiently support the growth of inoculated xenogenic ... Since their introduction almost a century ago, chick embryo model systems involving the technique of chorioallantoic grafting ...
"Aquaporin-1 expression in the chick embryo chorioallantoic membrane",. abstract = "The chick embryo chorioallantoic membrane ( ... The chick embryo chorioallantoic membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis. ... Aquaporin-1 expression in the chick embryo chorioallantoic membrane. In: Anatomical Record. 2002 ; Vol. 268, No. 2. pp. 85-89. ... N2 - The chick embryo chorioallantoic membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis ...
Furthermore, the anti-angiogenesis activity of C11 was verified in endothelial cells and chicken chorioallantoic membranes ( ... Chicken chorioallantoic membrane (CAM) assay. Groups of 10 fertilized chicken eggs were incubated in a humidified egg incubator ... Furthermore, the anti-angiogenesis activity of C11 was verified in endothelial cells and chicken chorioallantoic membranes ( ... c Anti-angiogenic effects of C11 in a chorioallantoic membrane (CAM) model. A glass cover-slip saturated with C11 or normal ...
Chick chorioallantoic membrane assays (CAM assay). Fertilized chicken eggs were incubated in incubator at 37.8 °C with 60-65% ... A total of 1 × 106 cells were inoculated atop the chick chorioallantoic membrane (CAM) and the window was resealed with ... Furthermore, we observed the effect of β-lap on breast cancer cell invasion by chorioallantoic membrane (CAM) invasion assay ... well transwells which containing an 8-mm pore size poly-carbonate membrane with or without a Matrigel-coated membrane matrix ...
chicken chorioallantoic membrane;. MMP,. matrix metalloproteinase;. HUVECs,. human umbilical vein endothelial cells;. LTR,. ... Chicken Chorioallantoic Membrane (CAM) Assay.. CAM assays were carried out as described (17, 27). After cutting a window in the ... membrane type 1 MMP;. SIN,. self-inactivating;. TIMP-2,. tissue inhibitor of metalloproteinase 2;. bFGF,. basic fibroblast ... The lower surface of the membranes was coated with collagen I (10 μg/ml) for 2 h at 37°C. Early-passage (p3-7) human umbilical ...
chorioallantoic membrane;. MMP,. matrix metalloproteinase;. VN,. vitronectin;. bVN,. biotinylated VN;. HRP,. horseradish ... Chick Chorioallantoic Membrane (CAM) Angiogenesis Assay.. Angiogenesis was assessed essentially as described previously (5, 6 ... Equal quantities of protein were separated by SDS/PAGE under reducing conditions and electroblotted to an Immobilon-P membrane ... For example, MMP2 and its activator membrane type 1 (MT1)-MMP are coordinately expressed by mesenchymal cells almost ...
... the chorioallantoic membrane and the zebrafish embryo neovascularization assays, and by the ex vivo mouse aortic ring assay. ... In vivo chorioallantoic membrane (CAM) and live fluorescent zebrafish embryo assays were carried out with different doses of ... The chicken chorioallantoic membrane (CAM) assay, a widely used and accessible system to study angiogenesis, was used to ... Chorioallantoic Membrane (CAM) Assay. Fertilized chick eggs were incubated horizontally at 38 °C in a humidified incubator, ...
Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane. Clinical and Experimental ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ...
  • [1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell. (wikipedia.org)
  • The authors studied the specific tumor antigen in Crocker's sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing chick embryo. (springer.com)
  • We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. (usask.ca)
  • After warming, the vitrified tissues were either immediately placed in 10% formalin (control) or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. (usask.ca)
  • This core facility provides scientific expertise and services using the chick embryo model specifically, its chorioallantoic membrane (CAM). (ipatimup.pt)
  • [2] As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen , to developing embryos . (wikipedia.org)
  • The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. (usask.ca)
  • The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes , such as birds and reptiles . (wikipedia.org)
  • Pathogenicity of Nocardia for chorio-allantoic membrane of the chick embryo. (bvsalud.org)
  • Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. (biomedcentral.com)
  • The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform preclinical oncological model for observing pro- and antiangiogenic response, tumor biology and metastasis. (biomedcentral.com)
  • Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis. (mysciencework.com)
  • The ability of the chick embryo's chorioallantoic membrane (CAM) to efficiently support the growth of inoculated xenogenic tumor cells greatly facilitates analysis of human tumor cell metastasis. (mysciencework.com)
  • The download The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The Web and the crisis tools differed an mistake that would navigate monopoly in the p., a method that refers up the interaction and the day. (itsys-rw.de)
  • The applications produced Invented for download The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM or Comment operations at days, production fields and countries. (itsys-rw.de)
  • The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. (usask.ca)
  • Upon initiation of angiogenesis with basic fibroblast growth factor (bFGF) on the chick chorioallantoic membrane (CAM), endothelial cell mitogen-activated protein (MAP) kinase (ERK) activity was detected as early as 5 min yet was sustained for at least 20 h. (rupress.org)
  • Optimising the chick chorioallantoic membrane xenograft model of neuroblastoma for drug delivery. (pubfacts.com)
  • The chicken embryo and the blood-vessel rich chorioallantoic membrane (CAM) is a valuable in vivo model to investigate biomedical processes, new ultrasound pulsing schemes, or novel transducers for contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery. (jove.com)
  • Chick embryo chorioallantoic membrane model systems to study a. (mysciencework.com)
  • Since their introduction almost a century ago, chick embryo model systems involving the technique of chorioallantoic grafting have proved invaluable in the in vivo studies of tumor development and angiogenesis and tumor cell dissemination. (mysciencework.com)
  • In this thesis the chicken chorioallantoic membrane (CAM) model was used to study the enhanced delivery of intravenously injected \SI{2}{\mega\dalton} FITC-dextrans using ultrasound in combination with microbubbles. (ntnu.no)
  • Chicken chorioallantoic membrane (CAM) models have been applied as an affordable and efficient angiogenesis model for many years in relevant areas such as cancer angiogenesis and cardiovascular sciences. (vascularcell.com)
  • In the present work, the effects of three AChE inhibitors donepezil, rivastigmine, and galantamine were tested on H2O2-induced apoptosis in human umbilical vein endothelial cells HUVECs and on angiogenesis in chicken chorioallantoic membrane model. (duhnnae.com)
  • As such, the purpose of this study was to investigate the antiangiogenic effects of Amiodarone HCl in the chorioallantoic membrane model. (cumhuriyet.edu.tr)
  • JNJ-26076713 blocks cell migration induced by vascular endothelial growth factor, fibroblast growth factor (FGF), and serum, and angiogenesis induced by FGF in the chick chorioallantoic membrane model. (aspetjournals.org)
  • Features of the model chorioallantoic membrane. (ebscohost.com)
  • Angiogenic potential of Aleurites moluccana (L.) Wild (Lumbang) crude bark and seed extracts on the chorioallantoic membrane (CAM) of a 10-day old chic embryo. (edu.ph)
  • Objective: The aim was to compare the irritation potency of frequently used wound antiseptics by using the hen's egg test on the chorioallantoic membrane (HET-CAM). (uni-greifswald.de)
  • Effect of ethyl acetate (EtOAc) fraction of V. tricolor on the number and diameter of vessels in chorioallantoic membrane (CAM). (hindawi.com)
  • For the purpose of determining the antiangiogenic effects of the drugs, blood vessels of the chorioallantoic membranes were evaluated using a stereoscopic microscope. (cumhuriyet.edu.tr)
  • Specifically, we investigated the ability of integrin αvβ3 and bFGF to influence MAP kinase activity in angiogenic blood vessels within the chick chorioallantoic membrane (CAM). (rupress.org)
  • Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg. (wikipedia.org)
  • At day 14, the capillary plexus is located at the surface of the ectoderm adjacent to the shell membrane. (alpfmedical.info)
  • The beak is against the inner shell membrane, ready to pierce it. (thepoultrysite.com)
  • The chick pierces the inner shell membrane and breathes in the air cell. (thepoultrysite.com)
  • Role of FGF-2 in chorioallantoic membrane vascularization 4. (moluna.de)
  • When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. (royalsocietypublishing.org)
  • To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on angiogenesis and growth of endometriosis-like lesions in chick en embryo chorioallantoic membrane. (bvsalud.org)
  • By days 12-15, the first visible lesions - small reddish spots called enanthem - appeared on mucous membranes of the mouth, tongue, palate , and throat, and temperature fell to near-normal. (wikipedia.org)
  • Fifteen Influenza A virus reference subtypes, H1 to H15, infected chicken embryonated chorioallantoic membranes (CAM) fixed by formalin, were all detectable in WF-4 based IHC. (flutrackers.com)
  • After warming, the vitrified tissues were either immediately placed in 10% formalin (control) or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. (usask.ca)
  • A look at his publications reveals his ability to use novel approaches in diverse areas of reproductive biology (e.g., embryology, placentation, and fetal membranes), ecological physiology, hibernation, and the integumentary, urinary, and digestive systems. (wikipedia.org)
  • The authors studied the specific tumor antigen in Crocker's sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing chick embryo. (springer.com)
  • Here, we studied the use of the chick chorioallantoic membrane (CAM) for the generation of human BL xenograft tumors, which we compared with known characteristics of the human disease. (beds.ac.uk)
  • For example, MMP2 and its activator membrane type 1 (MT1)-MMP are coordinately expressed by mesenchymal cells almost exclusively during embryonic development, indicating specific matrix remodeling constraints in these tissues ( 1 ). (pnas.org)
  • This photograph reveals smallpox virus pocks on the chorioallantoic membrane of a developing embryonic chick. (tolweb.org)
  • Poxviruses are very easy to isolate, and will grow in a variety of cell cultures, producing characteristic hemorrhagic pocks on the embryonic chicken chorioallantoic membrane (CAM). (tolweb.org)
  • [1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell. (wikipedia.org)
  • 1985) Electron download The Chick Embryo Chorioallantoic etc. of return subscription scores in the Jet Rock Formation( Lower Toarcian) of the Whitby accretion, UK. (itsys-rw.de)
  • Instead, the membrane-anchored MMP, MT1-MMP, confers endothelial cells with the ability to express invasive and tubulogenic activity in a collagen-rich milieu, in vitro or in vivo, where it plays an indispensable role in driving neovessel formation. (rupress.org)
  • Sprouting is a multistep process involving the dissolution of the basement membrane, the migration and proliferation of endothelial cells, the formation of a lumen and maturation of the new vessel with investment of the vessel with pericytes [ 3 ]. (mdpi.com)
  • Invasion of the membrane led to death of the embryo due to damage of the CAM, which could be examined histologically to show cell distribution and morphology, and by RT-PCR for assessment of patterns of fungal gene expression in vivo. (elsevier.com)
  • represents an experimentally tractable and inexpensive alternative to rodent or tissue culture-based invasion models, and can be used to investigate fungal pathogenesis and the genetic regulation of infection and membrane penetration of C albicans. (elsevier.com)
  • The protocol also describes how to inject cavitation nuclei, such as microbubbles, into the CAM vascular system, how to separate the membrane containing the embryo and CAM from the rest of the egg content for optically transparent studies, and how to use the chicken embryo and CAM in a variety of short-term ultrasound experiments. (jove.com)
  • Extracellular matrix-degradative enzymes, including the matrix metalloproteinases (MMPs) MMP-2 and MMP-9, are thought to play key roles in angiogenesis by binding to docking sites on the cell surface after activation by plasmin- and/or membrane-type (MT) 1-MMP-dependent processes. (rupress.org)
  • Furthermore, possible side effects such as membrane irritation, toxic, and anticoagulant properties of the investigated material in question can be detected. (uni-regensburg.de)
  • Conclusions: The chorioallantoic membrane culture system can be used to sustain the growth and development of isolated chick and quail lung buds, thus permitting their experimental manipulation to determine the role of different cell populations in lung development. (caltech.edu)
  • Lentiviral delivery of PEX blocked basic fibroblast growth factor-induced matrix metalloproteinase 2 activation and angiogenesis on chicken chorioallantoic membranes. (pnas.org)
  • The chorioallantoic membrane vascular growth 3. (moluna.de)
  • Fluorescently labeled murine mammary carcinoma cells, isolated from either MMTV-PyVmT transforming growth factor-beta receptor II knockout (TβRII KO) or TβRII fl/fl control mice, were combined with mammary fibroblasts and xenografted onto the chicken embryo chorioallantoic membrane. (biomedcentral.com)
  • We conclude that mechanical forces produced by blood flow have both a limiting and permissive influence on pillar development in the chick chorioallantoic membrane. (biomedcentral.com)