A highly vascularized extra-embryonic membrane, formed by the fusion of the CHORION and the ALLANTOIS. It is mostly found in BIRDS and REPTILES. It serves as a model for studying tumor or cell biology, such as angiogenesis and TISSUE TRANSPLANTATION.
An extra-embryonic membranous sac derived from the YOLK SAC of REPTILES; BIRDS; and MAMMALS. It lies between two other extra-embryonic membranes, the AMNION and the CHORION. The allantois serves to store urinary wastes and mediate exchange of gas and nutrients for the developing embryo.
The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.
A hard or leathery calciferous exterior covering of an egg.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The capability of producing eggs (OVA) from which young are hatched outside the body. While mostly referring to nonmammalian species, this does include MAMMALS of the order MONOTREMATA.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
A species of ORTHOPOXVIRUS causing infections in humans. No infections have been reported since 1977 and the virus is now believed to be virtually extinct.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Endothelial cells that line venous vessels of the UMBILICAL CORD.
A solid tumor consisting of a dense infiltration of MAST CELLS. It is generally benign.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A family of double-stranded DNA viruses infecting mammals (including humans), birds and insects. There are two subfamilies: CHORDOPOXVIRINAE, poxviruses of vertebrates, and ENTOMOPOXVIRINAE, poxviruses of insects.
The minute vessels that connect the arterioles and venules.
A genus of BIRDS in the family Phasianidae, order GALLIFORMES, containing the common European and other Old World QUAIL.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Virus diseases caused by the POXVIRIDAE.
A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
A cell line derived from cultured tumor cells.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Established cell cultures that have the potential to propagate indefinitely.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The development of the PLACENTA, a highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products between mother and FETUS. The process begins at FERTILIZATION, through the development of CYTOTROPHOBLASTS and SYNCYTIOTROPHOBLASTS, the formation of CHORIONIC VILLI, to the progressive increase in BLOOD VESSELS to support the growing fetus.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.
Transplantation between animals of different species.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Proteins prepared by recombinant DNA technology.
An enzyme that catalyzes the endonucleolytic cleavage of pancreatic ribonucleic acids to 3'-phosphomono- and oligonucleotides ending in cytidylic or uridylic acids with 2',3'-cyclic phosphate intermediates. EC
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A non-fibrillar collagen found in BASEMENT MEMBRANE. The C-terminal end of the alpha1 chain of collagen type XVIII contains the ENDOSTATIN peptide, which can be released by proteolytic cleavage.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Elements of limited time intervals, contributing to particular results or situations.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Adherence of cells to surfaces or to other cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A mature haploid female germ cell extruded from the OVARY at OVULATION.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN).
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The outer of the three germ layers of an embryo.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
The main trunk of the systemic arteries.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
Glycoproteins found on the membrane or surface of cells.
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
Signal molecules that are involved in the control of cell growth and differentiation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
Glycoproteins which have a very high polysaccharide content.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Transport proteins that carry specific substances in the blood or across cell membranes.
The rate dynamics in chemical or physical systems.
Cell membranes associated with synapses. Both presynaptic and postsynaptic membranes are included along with their integral or tightly associated specializations for the release or reception of transmitters.
Peptides composed of between two and twelve amino acids.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.

Inhibitory effects of 9-(4-thio-beta-D-ribo-pentofuranosyl)guanine on tumor growth and angiogenesis. (1/287)

BACKGROUND: To find a nucleoside with anti-angiogenic activity, we tried to screen an active compound from our nucleoside library. MATERIALS AND METHODS: The compound inhibiting the growth of human umbilical vein endothelial cell (HUVEC) induced by the conditioned medium of lung carcinoma cell line PC-9 was screened. The antitumor activity of the compound was evaluated against murine sarcoma S-180 implanted onto chick embryo chorioallantoic membrane (CAM). RESULTS: 9-(4-Thio-beta-D-ribo-pentofuranosyl)guanine (4'-thioguanosine) was found to be a potent inhibitor of the growth of HUVEC. The growth of S-180 implanted onto CAM was also inhibited by 4'-thioguanosine whereas the in vitro growth of S-180 was not inhibited. The administration of 4'-thioguanosine in mice caused unexpected side effect which suggested neurotoxicity. CONCLUSIONS: Antitumor effect of 4'-thioguanosine on S-180 was suggested to be due to inhibition of tumor angiogenesis. Because of toxicity of 4'-thioguanosine in mice, further development of the derivatives which have lower toxicity is required.  (+info)

Proangiogenic action of thyroid hormone is fibroblast growth factor-dependent and is initiated at the cell surface. (2/287)

The effects of thyroid hormone analogues on modulation of angiogenesis have been studied in the chick chorioallantoic membrane model. Generation of new blood vessels from existing vessels was increased 3-fold by either l-thyroxine (T4; 10(-7) mol/L) or 3,5,3'-triiodo-l-thyronine (10(-9) mol/L). T4-agarose reproduced the effects of T4, and tetraiodothyroacetic acid (tetrac) inhibited the effects of both T4 and T4-agarose. Tetrac itself was inactive and is known to block actions of T4 on signal transduction that are initiated at the plasma membrane. T4 and basic fibroblast growth factor (FGF2) were comparably effective as inducers of angiogenesis. Low concentrations of FGF2 combined with submaximal concentrations of T4 produced an additive angiogenic response. Anti-FGF2 inhibited the angiogenic effect of T4. The proangiogenic effects of T4 and FGF2 were blocked by PD 98059, a mitogen-activated protein kinase (MAPK) pathway inhibitor. Endothelial cells (ECV304) treated with T4 or FGF2 for 15 minutes demonstrated activation of MAPK, an effect inhibited by PD 98059 and the protein kinase C inhibitor CGP41251. Reverse transcription-polymerase chain reaction of RNA extracted from endothelial cells treated with T4 revealed increased abundance of FGF2 transcript at 6 to 48 hours, and after 72 hours, the medium of treated cells showed increased FGF2 content, an effect inhibited by PD 98059. Thus, thyroid hormone is shown to be a proangiogenic factor. This action, initiated at the plasma membrane, is MAPK dependent and mediated by FGF2.  (+info)

Effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenoviruses on antiangiogenesis. (3/287)

AIM: To investigate the inhibitory effects of endostatin-vascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization. METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane (CAM) model, rabbit inflammatory corneal neovascularization (CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo. RESULTS: Western blot showed that the molecular weight of fusion protein was about 41 kD after infection of ECV304, HepG2 and L929 cells with supernatant of AdhENDO-VEGI151. The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001), as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9+/-1849.9 mm(3)), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7+/-241.2 mm(3)) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75+/-3.31%) than in AdLacZ control (50.25+/-8.65%) (F=17.72, P=0.0056) with an inhibition rate of 39%. CONCLUSION: Fusion protein expressed by recombinant adenoviruses has a significant inhibitory effect on neovascularization.  (+info)

Regulation of neovascularization by human neutrophil peptides (alpha-defensins): a link between inflammation and angiogenesis. (4/287)

Angiogenesis, the growth of new blood vessels, is a complex biological process that is orchestrated by several growth factors and components of the extracellular matrix, including fibronectin (FN) and its receptor the integrin alpha5beta1. Angiogenesis is a critical part of inflammation and wound repair, but the mechanism by which vascular proliferation and migration is regulated by inflammatory cells is not completely understood. We have previously shown that human neutrophil peptides (HNPs), also known as alpha-defensins, which are secreted in high concentrations when neutrophils are activated, bind specifically to FN in the extracellular matrix and inhibit plasminogen activation. Therefore, we asked whether HNPs act as a link between inflammation and angiogenesis. Alpha5beta1-mediated endothelial cell adhesion and migration to FN, both under control conditions and under stimulation by vascular endothelial growth factor (VEGF), were inhibited specifically and in a dose-dependent manner by HNPs, whereas endothelial cell adhesion and migration to other components of the extracellular matrix, such as vitronectin, collagen, or fibrinogen/fibrin were not. Consistent with this finding, HNPs bound to and promoted the binding of fibronectin to alpha5beta1 integrin in arginine-glycine-aspartic acid (RGD)-independent manner. HNPs also completely inhibited VEGF-induced proliferation and induced apoptosis of endothelial cells in a dose-dependent manner. Moreover, HNPs inhibited capillary tube formation in three-dimensional fibrin-matrices as well as neovascularization in vivo in the chicken chorioallantoic membrane assay. Taken together, these data indicate that HNPs can regulate angiogenesis by affecting endothelial cell adhesion and migration in an FN-dependent manner as well as endothelial cell proliferation. These findings provide new insight into the role of inflammatory cells in angiogenesis and might provide a platform for developing a novel class of anti-angiogenesis drugs.  (+info)

Inhibiting MMP activity prevents the development of endometriosis in the chicken chorioallantoic membrane model. (5/287)

BACKGROUND: Matrix metalloproteinases (MMPs) are essential for extracellular matrix remodelling and may contribute to the development of endometriosis. Transplantation of endometrium onto the chicken chorioallantoic membrane (CAM) results in endometriosis-like lesion formation, a process that requires extensive tissue remodelling. We investigated the expression of a wide range of MMPs in menstrual endometrium, endometriosis-like lesions in CAMs, in peritoneal endometriosis and in endometriosis in the rectovaginal space, as well as the function of MMPs in early lesion formation in the CAM model. METHODS: Expression of MMPs was evaluated by immunohistochemistry and MMP function was studied in the CAM by inhibiting MMP activity during lesion formation. RESULTS: Nearly all MMPs were present in all tissues studied. No significant differences in the expression of a majority of MMPs were found in endometriosis-like lesions in CAMs when compared with human endometriosis. Inhibition of MMP-1, -2, -3, -7 and -13 activities significantly impaired endometriosis-like lesion formation in CAMs. CONCLUSIONS: The MMP expression profiles of experimentally induced endometriosis in CAMs and human endometriosis are similar. The prevention of endometriosis-like lesion formation in the CAM by inhibiting MMP activity strongly suggests that MMPs have a function in the early development of endometriotic lesions.  (+info)

Antiangiogenic activity of semisynthetic biotechnological heparins: low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists. (6/287)

OBJECTIVE: Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives. METHODS AND RESULTS: Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and alpha(v)beta3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/alpha(v)beta3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane. CONCLUSIONS: LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/alpha(v)beta3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.  (+info)

X-rays affect the expression of genes involved in angiogenesis. (7/287)

BACKGROUND: We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that are important regulators of angiogenesis: vascular endothelial growth factor (VEGF), heparin affin regulatory peptide (HARP) and inducible nitric oxide synthase (iNOS). MATERIALS AND METHODS: An area of 1 cm2 of the CAM, restricted by a plastic ring was irradiated at room temperature. The expression of the genes was studied using RT-PCR and the amounts of the mRNAs were quantified using image analysis of the corresponding agarose gels of the RT-PCR products. RESULTS: VEGF mRNA was decreased 6 h after irradiation. However, at later time points, VEGF expression was significantly increased compared with the nonirradiated tissue. Similarly, X-rays down-regulated both HARP and iNOS expression 6 h after irradiation and the effect was reversed at later time points, similarly to the effect of X-rays on VEGF. CONCLUSION: These data support the notion that X-rays increase the expression of genes that favor angiogenesis.  (+info)

Angiogenic response induced by acellular aortic matrix in vivo. (8/287)

In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a well-known angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-beta1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-beta1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-beta1 in the acellular matrices.  (+info)

Pathologic neovascularization can be seen in a variety of conditions, including cancer, diabetic retinopathy, and age-related macular degeneration. In cancer, for example, the formation of new blood vessels can help the tumor grow and spread to other parts of the body. In diabetic retinopathy, the growth of new blood vessels in the retina can cause vision loss and other complications.

There are several different types of pathologic neovascularization, including:

* Angiosarcoma: a type of cancer that arises from the cells lining blood vessels
* Hemangiomas: benign tumors that are composed of blood vessels
* Cavernous malformations: abnormal collections of blood vessels in the brain or other parts of the body
* Pyogenic granulomas: inflammatory lesions that can form in response to trauma or infection.

The diagnosis of pathologic neovascularization is typically made through a combination of physical examination, imaging studies (such as ultrasound, CT scans, or MRI), and biopsy. Treatment options vary depending on the underlying cause of the condition, but may include medications, surgery, or radiation therapy.

In summary, pathologic neovascularization is a process that occurs in response to injury or disease, and it can lead to serious complications. It is important for healthcare professionals to be aware of this condition and its various forms in order to provide appropriate diagnosis and treatment.

There are two main types of mastocytoma: cutaneous mastocytoma, which affects the skin, and systemic mastocytosis, which can affect multiple organs throughout the body. Cutaneous mastocytoma typically appears as a firm, raised bump or nodule on the skin, and may be accompanied by itching or other symptoms. Systemic mastocytosis is more serious and can cause a wide range of symptoms, including abdominal pain, diarrhea, fatigue, and anemia.

The exact cause of mastocytoma is not known, but it is believed to be linked to genetic mutations and environmental factors such as exposure to toxins or allergens. Diagnosis is typically made through a combination of physical examination, biopsy, and laboratory tests. Treatment options for mastocytoma include surgery, chemotherapy, and medications to reduce histamine levels.

Prognosis for mastocytoma varies depending on the type and severity of the disease, but in general, the prognosis is good for most patients with this condition. With proper treatment, many patients can achieve long-term remission or even be cured. However, in some cases, mastocytoma can progress to more aggressive forms of cancer, such as mast cell leukemia, which can be difficult to treat and has a poorer prognosis.

There is no specific treatment for poxviridae infections, but antiviral medications may be used in some cases. Vaccination against smallpox, which is caused by the variola virus, a member of the poxviridae family, has been widely used to prevent this disease and has been declared eradicated by the World Health Organization (WHO). However, other poxviridae infections remain a significant public health concern, particularly in areas where there is limited access to medical care.

Prevention of poxviridae infections involves avoiding contact with infected animals or people, wearing protective clothing and equipment, and practicing good hygiene. In addition, vaccination against specific poxviridae infections, such as monkeypox and cowpox, may be recommended for certain individuals who are at high risk of infection.

Overall, while the eradication of smallpox is a significant achievement, there remains much work to be done to prevent and control poxviridae infections, particularly in areas with limited access to medical care.

Types of experimental neoplasms include:

* Xenografts: tumors that are transplanted into animals from another species, often humans.
* Transgenic tumors: tumors that are created by introducing cancer-causing genes into an animal's genome.
* Chemically-induced tumors: tumors that are caused by exposure to certain chemicals or drugs.

The use of experimental neoplasms in research has led to significant advances in our understanding of cancer biology and the development of new treatments for the disease. However, the use of animals in cancer research is a controversial topic and alternatives to animal models are being developed and implemented.

2. Our research focuses on identifying the genetic mutations that contribute to experimental melanoma and developing targeted therapies.
3. The patient's experimental melanoma had spread to her lungs and liver, so we recommended chemotherapy and immunotherapy treatments.

The tumor cells are typically small, uniform, and well-differentiated, with a distinct cell border and a central nucleus. The tumor cells are often arranged in a glandular or tubular pattern, which is characteristic of this type of cancer.

Carcinoma, Lewis lung usually affects older adults, with the median age at diagnosis being around 60 years. Men are slightly more likely to be affected than women. The main risk factor for developing this type of cancer is smoking, although it can also occur in people who have never smoked.

The symptoms of Carcinoma, Lewis lung can vary depending on the location and size of the tumor, but they may include:

* Chest pain or discomfort
* Coughing up blood
* Shortness of breath
* Fatigue
* Weight loss

If you suspect you may have Carcinoma, Lewis lung or are experiencing any of these symptoms, it is important to consult a healthcare professional for an accurate diagnosis and appropriate treatment.

The post Definition of 'Carcinoma, Lewis Lung' in the medical field appeared first on Healthy Life Tips.

Neoplastic metastasis can occur in any type of cancer but are more common in solid tumors such as carcinomas (breast, lung, colon). It is important for cancer diagnosis and prognosis because metastasis indicates that the cancer has spread beyond its original site and may be more difficult to treat.

Metastases can appear at any distant location but commonly found sites include the liver, lungs, bones, brain, and lymph nodes. The presence of metastases indicates a higher stage of cancer which is associated with lower survival rates compared to localized cancer.

1) They share similarities with humans: Many animal species share similar biological and physiological characteristics with humans, making them useful for studying human diseases. For example, mice and rats are often used to study diseases such as diabetes, heart disease, and cancer because they have similar metabolic and cardiovascular systems to humans.

2) They can be genetically manipulated: Animal disease models can be genetically engineered to develop specific diseases or to model human genetic disorders. This allows researchers to study the progression of the disease and test potential treatments in a controlled environment.

3) They can be used to test drugs and therapies: Before new drugs or therapies are tested in humans, they are often first tested in animal models of disease. This allows researchers to assess the safety and efficacy of the treatment before moving on to human clinical trials.

4) They can provide insights into disease mechanisms: Studying disease models in animals can provide valuable insights into the underlying mechanisms of a particular disease. This information can then be used to develop new treatments or improve existing ones.

5) Reduces the need for human testing: Using animal disease models reduces the need for human testing, which can be time-consuming, expensive, and ethically challenging. However, it is important to note that animal models are not perfect substitutes for human subjects, and results obtained from animal studies may not always translate to humans.

6) They can be used to study infectious diseases: Animal disease models can be used to study infectious diseases such as HIV, TB, and malaria. These models allow researchers to understand how the disease is transmitted, how it progresses, and how it responds to treatment.

7) They can be used to study complex diseases: Animal disease models can be used to study complex diseases such as cancer, diabetes, and heart disease. These models allow researchers to understand the underlying mechanisms of the disease and test potential treatments.

8) They are cost-effective: Animal disease models are often less expensive than human clinical trials, making them a cost-effective way to conduct research.

9) They can be used to study drug delivery: Animal disease models can be used to study drug delivery and pharmacokinetics, which is important for developing new drugs and drug delivery systems.

10) They can be used to study aging: Animal disease models can be used to study the aging process and age-related diseases such as Alzheimer's and Parkinson's. This allows researchers to understand how aging contributes to disease and develop potential treatments.

Neoplasm refers to an abnormal growth of cells that can be benign (non-cancerous) or malignant (cancerous). Neoplasms can occur in any part of the body and can affect various organs and tissues. The term "neoplasm" is often used interchangeably with "tumor," but while all tumors are neoplasms, not all neoplasms are tumors.

Types of Neoplasms

There are many different types of neoplasms, including:

1. Carcinomas: These are malignant tumors that arise in the epithelial cells lining organs and glands. Examples include breast cancer, lung cancer, and colon cancer.
2. Sarcomas: These are malignant tumors that arise in connective tissue, such as bone, cartilage, and fat. Examples include osteosarcoma (bone cancer) and soft tissue sarcoma.
3. Lymphomas: These are cancers of the immune system, specifically affecting the lymph nodes and other lymphoid tissues. Examples include Hodgkin lymphoma and non-Hodgkin lymphoma.
4. Leukemias: These are cancers of the blood and bone marrow that affect the white blood cells. Examples include acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL).
5. Melanomas: These are malignant tumors that arise in the pigment-producing cells called melanocytes. Examples include skin melanoma and eye melanoma.

Causes and Risk Factors of Neoplasms

The exact causes of neoplasms are not fully understood, but there are several known risk factors that can increase the likelihood of developing a neoplasm. These include:

1. Genetic predisposition: Some people may be born with genetic mutations that increase their risk of developing certain types of neoplasms.
2. Environmental factors: Exposure to certain environmental toxins, such as radiation and certain chemicals, can increase the risk of developing a neoplasm.
3. Infection: Some neoplasms are caused by viruses or bacteria. For example, human papillomavirus (HPV) is a common cause of cervical cancer.
4. Lifestyle factors: Factors such as smoking, excessive alcohol consumption, and a poor diet can increase the risk of developing certain types of neoplasms.
5. Family history: A person's risk of developing a neoplasm may be higher if they have a family history of the condition.

Signs and Symptoms of Neoplasms

The signs and symptoms of neoplasms can vary depending on the type of cancer and where it is located in the body. Some common signs and symptoms include:

1. Unusual lumps or swelling
2. Pain
3. Fatigue
4. Weight loss
5. Change in bowel or bladder habits
6. Unexplained bleeding
7. Coughing up blood
8. Hoarseness or a persistent cough
9. Changes in appetite or digestion
10. Skin changes, such as a new mole or a change in the size or color of an existing mole.

Diagnosis and Treatment of Neoplasms

The diagnosis of a neoplasm usually involves a combination of physical examination, imaging tests (such as X-rays, CT scans, or MRI scans), and biopsy. A biopsy involves removing a small sample of tissue from the suspected tumor and examining it under a microscope for cancer cells.

The treatment of neoplasms depends on the type, size, location, and stage of the cancer, as well as the patient's overall health. Some common treatments include:

1. Surgery: Removing the tumor and surrounding tissue can be an effective way to treat many types of cancer.
2. Chemotherapy: Using drugs to kill cancer cells can be effective for some types of cancer, especially if the cancer has spread to other parts of the body.
3. Radiation therapy: Using high-energy radiation to kill cancer cells can be effective for some types of cancer, especially if the cancer is located in a specific area of the body.
4. Immunotherapy: Boosting the body's immune system to fight cancer can be an effective treatment for some types of cancer.
5. Targeted therapy: Using drugs or other substances to target specific molecules on cancer cells can be an effective treatment for some types of cancer.

Prevention of Neoplasms

While it is not always possible to prevent neoplasms, there are several steps that can reduce the risk of developing cancer. These include:

1. Avoiding exposure to known carcinogens (such as tobacco smoke and radiation)
2. Maintaining a healthy diet and lifestyle
3. Getting regular exercise
4. Not smoking or using tobacco products
5. Limiting alcohol consumption
6. Getting vaccinated against certain viruses that are associated with cancer (such as human papillomavirus, or HPV)
7. Participating in screening programs for early detection of cancer (such as mammograms for breast cancer and colonoscopies for colon cancer)
8. Avoiding excessive exposure to sunlight and using protective measures such as sunscreen and hats to prevent skin cancer.

It's important to note that not all cancers can be prevented, and some may be caused by factors that are not yet understood or cannot be controlled. However, by taking these steps, individuals can reduce their risk of developing cancer and improve their overall health and well-being.

1. Tumor size and location: Larger tumors that have spread to nearby tissues or organs are generally considered more invasive than smaller tumors that are confined to the original site.
2. Cellular growth patterns: The way in which cancer cells grow and divide can also contribute to the overall invasiveness of a neoplasm. For example, cells that grow in a disorganized or chaotic manner may be more likely to invade surrounding tissues.
3. Mitotic index: The mitotic index is a measure of how quickly the cancer cells are dividing. A higher mitotic index is generally associated with more aggressive and invasive cancers.
4. Necrosis: Necrosis, or the death of cells, can be an indication of the level of invasiveness of a neoplasm. The presence of significant necrosis in a tumor is often a sign that the cancer has invaded surrounding tissues and organs.
5. Lymphovascular invasion: Cancer cells that have invaded lymphatic vessels or blood vessels are considered more invasive than those that have not.
6. Perineural invasion: Cancer cells that have invaded nerve fibers are also considered more invasive.
7. Histological grade: The histological grade of a neoplasm is a measure of how abnormal the cancer cells look under a microscope. Higher-grade cancers are generally considered more aggressive and invasive than lower-grade cancers.
8. Immunohistochemical markers: Certain immunohistochemical markers, such as Ki-67, can be used to evaluate the proliferative activity of cancer cells. Higher levels of these markers are generally associated with more aggressive and invasive cancers.

Overall, the degree of neoplasm invasiveness is an important factor in determining the likelihood of the cancer spreading to other parts of the body (metastasizing) and in determining the appropriate treatment strategy for the patient.

It is the outermost extra-embryonic membrane which lines the non-vascular egg shell membrane. The chorioallantoic membrane is ... The Chorioallantoic Membrane (CAM), also known as the chorioallantois, is a highly vascularized membrane found in the eggs of ... Both the epithelial layers are separated from the mesodermal layer by basement membranes. The Chorioallantoic membrane performs ... Chorioallantoic membranes can be cultivated either outside (ex-ovo) or inside of the shell (in-ovo). Here, the embryo is grown ...
Promotes Angiogenesis in the Quail Chorioallantoic Membrane". Endothelium. 13 (1): 51-59. doi:10.1080/10623320600669053. PMID ...
... virus lesions on the chorioallantoic membrane of a developing chick. In contrast to the rash in smallpox, the rash in ... Definitive laboratory identification of variola virus involved growing the virus on chorioallantoic membrane (part of a chicken ... A rash developed on the skin 24 to 48 hours after lesions on the mucous membranes appeared. Typically the macules first ... Hemorrhages in the mucous membranes appear to occur less often than in the early hemorrhagic form. Sometimes the rash forms ...
The Ankara strain was taken to West Germany in 1953, where Herrlich and Mayr grew it on chorioallantoic membrane at the ... Second-generation vaccines were grown in chorioallantoic membrane or cell cultures for greater purity, and they were used in ... Goodpasture, E. W.; Woodruff, Alice M.; Buddingh, G. J. (May 1932). "Vaccinal Infection of the Chorio-Allantoic Membrane of the ... Ernest William Goodpasture, Alice Miles Woodruff, and G. John Buddingh grew vaccinia virus on the chorioallantoic membrane of ...
Rangan, S. R. S.; Sirsat, Satyavati M. (1962-03-01). "The Fine Structure of the Normal Chorio-Allantoic Membrane of the Chick- ...
... the intimate interaction of the uterus and a series of embryonic membranes including the chorioallantoic and yolk sac membranes ... "Comparative genomics of hormonal signaling in the chorioallantoic membrane of oviparous and viviparous amniotes". Gen Comp ... The placenta can be defined as an organ formed by the sustained apposition or fusion of fetal membranes and parental tissue for ... communication occurs via the production of a range of signalling molecules and their receptors in the chorioallantoic membrane ...
Woodruff, Alice Miles; Goodpasture, Ernest W. (May 1931). "The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos ... "The Cultivation of Vaccine and Other Viruses in the Chorioallantoic Membrane of Chick Embryos". Science. 74 (1919): 371-372. ...
"The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos". Science. 74 (1919): 371-72. ... In the past, fertile hens' eggs were used and the viruses were grown on the membranes surrounding the embryo. This method is ... The FFA is particularly useful for quantifying classes of viruses that do not lyse the cell membranes, as these viruses would ...
Maitland HB, Magrath DI (September 1957). "The growth in vitro of vaccinia virus in chick embryo chorio-allantoic membrane, ... "The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos". Science. 74 (1919): 371-2. ...
"The Cultivation Of Vaccine and other Viruses In The Chorioallantoic Membrane of Chick Embryos". Science. 74 (1919): 371-372. ... The causes of death include cell lysis (bursting), alterations to the cell's surface membrane and apoptosis (cell "suicide"). ...
In contrast to Sulf1, Sulf2 actually induced angiogenesis in a chick chorioallantoic membrane assay. Sulf2 was measured for its ... Vascular basement membrane (BM) HSPGs are modified to bind L-selectin and monocyte chemoattractant protein-1 (MCP-1) during ... In addition to cell membrane association, Sulfs also secreted freely into the media, which contrasts the findings with QSulf1 ... QSulf1 localizes exclusively to the cell surface by interacting hydrophilically with a non-heparan sulfate outer membrane ...
Domermuth, C. H.; Edwards, O. F. (1 January 1957). "An electron microscope study of chorioallantoic membrane infected with the ... It was also found that the pathogens could not be bacteria or protozoans as they passed through membranes (Berkefield filter) ...
During the incubation period, the virus replicates in the cells that make up the chorioallantoic membrane. In human vaccine ...
... specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane". Developmental ...
Wimsatt, W. A. Enders A. C.; Mossman, H. W. (Oct 1973). "A reexamination of the chorioallantoic placental membrane of a shrew, ... Wimsatt, W. A. (Aug 1974). "Morphogenesis of the fetal membranes and placenta of the black bear, Ursus americanus (Pallas)". Am ... Wimsatt, W. A. (1954). "The fetal membranes and placentation of the tropical American vampire bat Desmodus rotundus murinus, ... Wimsatt, W. A. (Jan 1949). "Cytochemical observations on the fetal membranes and placenta of the bat, Myotis lucifugus ...
... the chorion and the allantois fuse together to form the chorioallantoic membrane. Together these form a double membrane, which ... In reptiles, birds, and monotremes, the chorion is one of the four extraembryonic membranes that make up the amniotic egg that ... The chorion is the outermost fetal membrane around the embryo in mammals, birds and reptiles (amniotes). It develops from an ... In humans and other mammals (excluding monotremes), the chorion is one of the fetal membranes that exist during pregnancy ...
... specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane". Developmental ...
The chorioallantoic membrane (CAM), a well-vascularized extra-embryonic tissue located underneath the eggshell, has a ... Woodruff, A. M.; Goodpasture, E. W. (1931). "The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection ...
Woodruff, Alice Miles; Goodpasture, Ernest W. (May 1931). "The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos ...
He isolated the virus from monkey kidney tissue cell culture and from the chorioallantoic membrane of chick embryos. The ...
... bFGF induced vascularization in the corneal pocket assay and inhibit VEGF induced angiogenesis in the chorioallantoic membrane ... Shedding of a membrane-anchored cytokine or growth factor by ADAM proteinases may be relevant for various signal transduction ... Semaphorin 4D is cleaved from its membrane-bound form by MT1-MMP (MMP-14) in tumor cells; it then interacts with plexin B1 on ... The vascular basement membrane is composed of type IV collagen, type XV collagen, type XVIII collagen, laminins, entactin, ...
When inoculated on the chorioallantoic membrane (CAM) of 12-day embryonated hens' eggs, the RCN produced many small discrete ... Genome replication begins with the uncoating of the virus core as it comes into contact with the plasma membrane. Once uncoated ...
In South Africa, the Neethling strain of the virus was first attenuated by 20 passages on the chorio-allantoic membranes of ... Additionally, the nodules on the mucous membranes of the eyes, nose, mouth, rectum, udder and genitalia quickly ulcerate, ... on the skin and mucous membranes (including those of the respiratory and gastrointestinal tracts). Infected cattle also may ...
... past the chorioallantoic membrane. The two holes are then sealed with melted paraffin and the inoculated eggs are incubated for ...
Whereas the chorioallantoic membrane (CAM) assay shows the type I repeats of TSP1 to be antiangiogenic, it also shows that the ... Forslöw A, Liu Z, Sundqvist KG (Jan 2007). "Receptor communication within the lymphocyte plasma membrane: a role for the ...
... property of cucurbitacin E was demonstrated in vitro but also in vivo in a chick embryo chorioallantoic membrane and in a mouse ...
... a membrane in the amniotic sac Basement membrane, a thin sheet of fibers that underlies the epithelium Chorioallantoic membrane ... a triangular membrane occurring in eyes Cell membranes: Plasma membrane, a membrane that separates the interior of all cells ... a smooth membrane consisting of a thin layer of cells, which secrete serous fluid Tunic membrane, protective membrane covering ... like biological membranes. Membrane may also refer to: Look up membrane in Wiktionary, the free dictionary. Biology: Isolating ...
... describing the cultivation of the smallpox virus on the chorioallantoic membrane of hen's eggs. Dumbell was head of the ...
... chorioallantoic membrane MeSH A16.254.300.600 - cleavage stage, ovum MeSH A16.254.300.600.274 - blastula MeSH A16.254.300.600. ... vitelline membrane MeSH A16.631.900 - zona pellucida MeSH A16.759.189 - chorionic villi MeSH A16.759.289 - decidua MeSH A16.759 ... 550 - morula MeSH A16.254.300.800 - yolk sac MeSH A16.254.403 - extraembryonic membranes MeSH A16.254.403.147 - allantois MeSH ...
... when transplanted to the chorioallantoic membrane of a chick in a later stage of development, in "cut-and-paste" experiments. ... Reddick ML (1937). The differentiation of portions of the chick blastoderm in chorio-allantoic grafts. Thesis for Degree of the ... "The differentiation of portions of the chick blastoderm in chorio-allantoic grafts". Robert W. Woodruff Library. University of ... Her doctoral dissertation was titled The differentiation of embryonic chick medulla in chorioallantoic grafts. She was elected ...
... with a system that involved the inhibition of the growth of live influenza virus in chicken embryo chorioallantoic membranes by ... such as membrane bound toll like receptors or the cytoplasmic receptors RIG-I or MDA5, can trigger release of IFNs. Toll Like ... that this interference was mediated by a protein released by cells in the heat-inactivated influenza virus-treated membranes. ...
... with attached fetal membranes, ruptured at the margin at the left in the image Micrograph of a placental infection ( ... Placental mammals, including humans, have a chorioallantoic placenta that forms from the chorion and allantois. In humans, the ...
... egg chorioallantoic membrane (HET-CAM) assay and an epithelial model cultivated from human corneal cells, in comparison with ... "Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes". J. ...
... chorioallantoic membrane MeSH A10.615.284.473 - chorion MeSH A10.615.284.473.200 - chorionic villi MeSH A10.615.284.981 - yolk ... basement membrane MeSH A10.615.179.124 - basilar membrane MeSH A10.615.179.250 - bruch membrane MeSH A10.615.179.437 - descemet ... membrane MeSH A10.615.179.625 - glomerular basement membrane MeSH A10.615.284 - extraembryonic membranes MeSH A10.615.284.147 ... basement membrane MeSH A10.272.220.250 - bruch membrane MeSH A10.272.491 - endothelium MeSH A10.272.491.318 - endothelium, ...
... chorioallantoic membrane - choriocarcinoma - choroid plexus tumor - CHPP - chronic granulocytic leukemia - chronic idiopathic ... synovial membrane - synovial sarcoma - synthetic protegrin analog - synthetic retinoid - systemic chemotherapy - systemic ... mesenteric membrane - mesna - mesonephroma - mesothelioma - metaplasia - metaplastic carcinoma - metastasectomy - metastasis - ...
... proteins on cell surfaces Chorioallantoic membrane, in developing eggs Crassulacean acid metabolism, a plant carbon-fixing ...
... GNUTTI, ALESSANDRO. ... or anti-angiogenic responses induced by different molecules when implanted in vivo on the chick embryo chorioallantoic membrane ... or anti-angiogenic responses induced by different molecules when implanted in vivo on the chick embryo chorioallantoic membrane ...
Chorioallantoic Membrane Assay. The aqueous extract of MO leaves was tested on the chorioallantoic membrane in order to ... 4.3.4. Chorioallantoic Membrane Assay. The antiangiogenic potential of the aqueous extract of MO leaves was evaluated by using ... Ribatti, D. The chick embryo chorioallantoic membrane in the study of tumor angiogenesis. Rom. J. Morphol Embryol. 2008, 49, ... Figure 4. Stereomicroscopic images regarding the effects of the aqueous extracts of MO leaves on the chorioallantoic membrane ...
The chorioallantoic membrane (CAM) assay is a well-established technique to evaluate tumor invasion and angiogenesis and may ... Chorioallantoic membrane assay revealed the role of TIPARP (2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible poly (ADP-ribose) ... Chorioallantoic membrane assay revealed the role of TIPARP (2,3,7,8-tetrachlorodibenzo-p-d ... 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible poly (ADP-ribose) polymerase (TIPARP); Angiogenesis; Chorioallantoic membrane; ...
... 27 Sep, 2022 , Publications ... Among in vivo angiogenesis assays, chick chorioallantoic membrane (CAM) assay has allowed meaningful progress in elucidating ...
SMB; chorioallantoic membrane; serum Erythrocytes (species used). Several* pH Range. 6.5-8.5 pH Optimum. ...
On the 12th day, the images of chorioallantoic membrane samples were prepared using photostreomicroscope and the number and ... On the 12th day, the images of chorioallantoic membrane samples were prepared using photostreomicroscope and the number and ... synergic effect of glycyrrhizic acid and low frequency electromagnetic field on angiogenesis in chick chorioallantoic membrane ... The inhibitory effect of low frequency electromagnetic field (50Hz) on angiogenesis in chorioalantoic membrane of chick. J ...
The chorioallantoic membrane (CAM) of the chick egg has been extensively used for the growth of normal and transformed ... The chorioallantoic membrane (CAM) of the chick egg has been extensively used for the growth of normal and transformed ... The chorioallantoic membrane (CAM) of the chick egg has been extensively used for the growth of normal and transformed ... The chorioallantoic membrane (CAM) of the chick egg has been extensively used for the growth of normal and transformed ...
The first evidence of the tumor-induced angiogenesis in vivo by using the chorioallantoic membrane assay dated 1913 *Domenico ...
Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg ... Dive into the research topics of Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a ...
... on the chorioallantoic membrane (CAM). The effects on the vessels of a mucous membrane were directly assessed by ... on the chorioallantoic membrane (CAM). The effects on the vessels of a mucous membrane were directly assessed by ... Kishore AS, Surekha PA, Sekhar PV, et al.: Hen egg chorioallantoic membrane bioassay: an in vitro alternative to draize eye ... Luepke NP: Hens egg chorioallantoic membrane test for irritation potential. Food Chem Toxicol 1985, 23: 287-291. 10.1016/0278- ...
Chicken chorioallantoic membrane (CAM) assay. CAM assay was performed as described previously [52]. At 9 day of development, ... at the plasma membrane (Figure 2C). No alterations on the expression of other metabolic markers, such as hexokinase II (HKII) ... at the plasma membrane was observed in both cell lines (Figure 3B and 3C). Neither the transporter MCT4 (monocarboxylate ... mainly the preferential location of MCT1 at the plasma membrane. Few studies reported modulation of MCT isoforms expression ...
7. The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis.. Herrmann A; Moss D; Sée V. ... The chick embryo chorioallantoic membrane as an in vivo experimental model to study human neuroblastoma.. Ribatti D; Tamma R. J ... The chick chorioallantoic membrane as an in vivo angiogenesis model.. Ponce ML; Kleinmann HK. Curr Protoc Cell Biol; 2003 May; ... The chick embryo chorioallantoic membrane as a model system for the study of tumor angiogenesis, invasion and development of ...
Viruses were isolated in Vero cell culture and on chorioallantoic membrane (CAM) of embryonated chicken eggs. Among 21 samples ...
The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma ... The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma. Michael Li1, ...
Chorioallantoic membrane of the chicken embryo (CAM). a Bright-field image of the CAM vasculature presenting the functional ... Chorioallantoic membrane of the chicken embryo (CAM). a Bright-field image of the CAM… ... Keywords: Angiogenesis; Aortic ring; Chorioallantoic membrane (CAM); Corneal angiogenesis; Endothelial cell migration; Hindlimb ... Arrows indicate the capillary basement membrane. L indicates lumen while Nuc indicates nuclear labeling. Bar equals 25 μm ...
Chorioallantoic Membrane A16.254.300.400 A16.331.400. Chorion A16.254.403.473 A16.254.750.473. Chorionic Villi A16.254.403.473. ... Extraembryonic Membranes A16.254.403 A16.254.750. Fasciitis, Necrotizing C1.252.410.890.350 C1.252.825.340. Federal Government ... Vitelline Membrane A16.631.886 A16.690.886. Volatilization H1.181.529.952 H1.181.529.699.933. Waldenstrom Macroglobulinemia ... Tectorial Membrane A9.246.631.246.292.906 A9.246.631.246.292.938. Tegmentum Mesencephali A8.186.211.653.822. Telangiectasia, ...
In addition, an in vivo chicken chorioallantoic membrane (CAM) test was also performed. Aloe emodin performed in vivo ... measuring the membrane potential of mitochondria, which has a regulatory role in initiating apoptosis, is also important for ... After the administration of aloe emodin, it was observed that the mitochondrial membrane potential decreased in human tongue ... membrane bubbles, and nuclear fragmentation [25]. Pecere et al., in a later study, used neuroblastoma cell lines (SK-N-BE(2c) ...
Variola and vaccinia can be cultured in vitro on chorioallantoic membranes of eggs and in tissue culture. In suspected cases of ... and intraepithelial multiloculated vesicles develop by rupture of cellular membranes in the stratum spinosum. Cells develop ...
4. The chick embryo chorioallantoic membrane as a model for in vivo research on angiogenesis.. Ribatti D; Vacca A; Roncali L; ... A novel early chorioallantoic membrane assay demonstrates quantitative and qualitative changes caused by antiangiogenic ... Poly-L-lysine/heparin stimulates angiogenesis in chick embryo chorioallantoic membrane.. Pacini S; Gulisano M; Vannucchi S; ... VEGF-A promotes intussusceptive angiogenesis in the developing chicken chorioallantoic membrane.. Baum O; Suter F; Gerber B; ...
... induce VEGF expression and angiogenesis in both models of cell culture and chick chorioallantoic membrane. These findings ...
... stage tumor spheroid in chorioallantoic membrane model with upconversion nanoparticles. Nanoscale, 7(5), 1596-1600. https://doi ...
... in both models of cell culture and chick chorioallantoic membrane. These findings suggest that VEGF may be a mechanism whereby ...
WC-Co nanoparticles also induced angiogenesis as determined by the chicken chorioallantoic membrane (CAM) assay. Mechanistic ...
Determination of PCBs in eggs and chorioallantoic membranes of American alligators (Alligator mississippiensis). Environ ...
Antiangiogenic Study of Two Nonsteriodal Antiinflammatory Compounds Using Chick Chorioallantoic Membrane Assay. M.A. Mojid ...
  • Chorioallantoic Membrane (HET-CAM) assay. (nih.gov)
  • WC-Co nanoparticles also induced angiogenesis as determined by the chicken chorioallantoic membrane (CAM) assay. (cdc.gov)
  • Chick chorioallantoic membrane assay was carried out to examine the angiogenic potential of the chitosan binder combined with/without hMSCs. (qmul.ac.uk)
  • The oxygen sensitivity and phototoxicity of [Ru(Phen)] in chicken embryo chorioallantoic membrane was investigated. (sigmaaldrich.com)
  • The vascular irritation was evaluated by the hen's egg test (HET) on the chorioallantoic membrane (CAM). (biomedcentral.com)
  • The effects on the vessels of a mucous membrane were directly assessed by stereomicroscopic observation in vivo. (biomedcentral.com)
  • In addition, comparative in vivo studies will be performed on intestinal epithelial cells and tissues in an animal model of the chorioallantoic membrane (CAM) of chicken eggs. (nmi.de)
  • Collin-Osdoby, P., Rothe, L., Becker, S., Anderson, F., Osdoby, P. (2000) Decreased nitric oxide levels promote osteoclast formation and bone resorption in chick bone marrow and isolated osteoclast cultures in vitro as well as in vivo on the chick chorioallantoic membrane in conjunction with neoangiogenesis. (wustl.edu)
  • His work deals with themes such as Molecular biology, Chorioallantoic membrane, In vivo and Ribonuclease, which intersect with Angiogenin. (research.com)
  • We have also observed a marked anti-angiogenic effect of CAI in vivo, in chicken chorioallantoic membrane (CAM) assays. (nih.gov)
  • Moreover, RANTES promotes the expression of matrix metalloproteinase (MMP) 9 by THP-1 monocytic cells and elevates vascularity in chick chorioallantoic membrane assays. (nih.gov)
  • The inhibitory effect of low frequency electromagnetic field (50Hz) on angiogenesis in chorioalantoic membrane of chick. (ac.ir)
  • The chorioallantoic membrane (CAM) of the chick egg has been extensively used for the growth of normal and transformed mammalian tissues. (biu.ac.il)
  • This photograph reveals smallpox virus pocks on the chorioallantoic membrane (CAM) of a developing embryonic chick. (cdc.gov)
  • A highly vascularized extra-embryonic membrane, formed by the fusion of the CHORION and the ALLANTOIS . (nih.gov)
  • It lies between two other extra-embryonic membranes, the AMNION and the CHORION. (bvsalud.org)
  • Variola and vaccinia can be cultured in vitro on chorioallantoic membranes of eggs and in tissue culture. (medscape.com)
  • Epidermal cells develop ballooning degeneration, and intraepithelial multiloculated vesicles develop by rupture of cellular membranes in the stratum spinosum. (medscape.com)
  • Hen's egg chorioallantoic membrane (HET-CAM) test was used for the evaluation of ocular tolerance, which showed the non-irritant efficacy of the developed formulation. (ijpsr.com)
  • On the 12th day, the images of chorioallantoic membrane samples were prepared using photostreomicroscope and the number and length of vessels were measured. (ac.ir)
  • Endometrial and chorioallantoic membrane samples were collected from healthy and arresting conceptus attachment sites at gestation day (gd) 20 (n = 8) and gd 50 (n = 8). (biomedcentral.com)
  • Xenograft tumors can be grown rapidly on a highly vascularized structure known as the chorioallantoic membrane (CAM). (bmrat.org)
  • Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. (nih.gov)
  • Tube formation assay and chorioallantoic membranes assay were used to assess tumor angiogenesis. (nih.gov)
  • Chorioallantoic Membrane (HET-CAM) assay. (nih.gov)
  • 1. Establishment of an in ovo chick embryo yolk sac membrane (YSM) assay for pilot screening of potential angiogenic and anti-angiogenic agents. (nih.gov)
  • 3. A novel angiogenesis model for screening anti-angiogenic compounds: the chorioallantoic membrane/feather bud assay. (nih.gov)
  • 4. The chick embryo chorioallantoic membrane (CAM) assay. (nih.gov)
  • 9. Depletion of Embryonic Macrophages Leads to a Reduction in Angiogenesis in the Ex Ovo Chick Chorioallantoic Membrane Assay. (nih.gov)
  • 15. Chorioallantoic Membrane Assay as Model for Angiogenesis in Tissue Engineering: Focus on Stem Cells. (nih.gov)
  • WC-Co nanoparticles also induced angiogenesis as determined by the chicken chorioallantoic membrane (CAM) assay. (cdc.gov)
  • 20. In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane. (nih.gov)
  • Pocks morphology in chorioallantoic membranes. (cdc.gov)
  • The whitish pockmarks produced on chorioallantoic membranes resembled VACV pocks, differing from the red hemorrhagic ones produced by cowpox virus (CPXV) ( Figure A1 ). (cdc.gov)