Chorioallantoic Membrane: A highly vascularized extra-embryonic membrane, formed by the fusion of the CHORION and the ALLANTOIS. It is mostly found in BIRDS and REPTILES. It serves as a model for studying tumor or cell biology, such as angiogenesis and TISSUE TRANSPLANTATION.Allantois: An extra-embryonic membranous sac derived from the YOLK SAC of REPTILES; BIRDS; and MAMMALS. It lies between two other extra-embryonic membranes, the AMNION and the CHORION. The allantois serves to store urinary wastes and mediate exchange of gas and nutrients for the developing embryo.Chorion: The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.Chick Embryo: The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.Extraembryonic Membranes: The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.Egg Shell: A hard or leathery calciferous exterior covering of an egg.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Allantoin: A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Angiogenesis Inhibitors: Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.Angiogenesis Inducing Agents: Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.Membranes: Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Membrane Lipids: Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Intracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.Oviparity: The capability of producing eggs (OVA) from which young are hatched outside the body. While mostly referring to nonmammalian species, this does include MAMMALS of the order MONOTREMATA.Membrane Potentials: The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Membranes, Artificial: Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.Blood Vessels: Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).Variola virus: A species of ORTHOPOXVIRUS causing infections in humans. No infections have been reported since 1977 and the virus is now believed to be virtually extinct.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Quail: Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.Umbilical Veins: Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.Human Umbilical Vein Endothelial Cells: Endothelial cells that line venous vessels of the UMBILICAL CORD.Mastocytoma: A solid tumor consisting of a dense infiltration of MAST CELLS. It is generally benign.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Poxviridae: A family of double-stranded DNA viruses infecting mammals (including humans), birds and insects. There are two subfamilies: CHORDOPOXVIRINAE, poxviruses of vertebrates, and ENTOMOPOXVIRINAE, poxviruses of insects.Capillaries: The minute vessels that connect the arterioles and venules.Coturnix: A genus of BIRDS in the family Phasianidae, order GALLIFORMES, containing the common European and other Old World QUAIL.Placenta: A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Poxviridae Infections: Virus diseases caused by the POXVIRIDAE.Orthomyxoviridae: A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.Erythrocyte Membrane: The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.Membrane Fluidity: The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Endothelial Growth Factors: These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.Vascular Endothelial Growth Factor Receptor-2: A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.Vascular Endothelial Growth Factors: A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.Neoplasms, Experimental: Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.Cell Line, Tumor: A cell line derived from cultured tumor cells.Lymphokines: Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Placentation: The development of the PLACENTA, a highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products between mother and FETUS. The process begins at FERTILIZATION, through the development of CYTOTROPHOBLASTS and SYNCYTIOTROPHOBLASTS, the formation of CHORIONIC VILLI, to the progressive increase in BLOOD VESSELS to support the growing fetus.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Viviparity, Nonmammalian: The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.Transplantation, Heterologous: Transplantation between animals of different species.Basement Membrane: A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Ribonuclease, Pancreatic: An enzyme that catalyzes the endonucleolytic cleavage of pancreatic ribonucleic acids to 3'-phosphomono- and oligonucleotides ending in cytidylic or uridylic acids with 2',3'-cyclic phosphate intermediates. EC 3.1.27.5.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Collagen Type XVIII: A non-fibrillar collagen found in BASEMENT MEMBRANE. The C-terminal end of the alpha1 chain of collagen type XVIII contains the ENDOSTATIN peptide, which can be released by proteolytic cleavage.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Laminin: Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.Melanoma, Experimental: Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.Carcinoma, Lewis Lung: A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.Xenograft Model Antitumor Assays: In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Receptors, Vitronectin: Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Endostatins: Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII.Neoplasm Transplantation: Experimental transplantation of neoplasms in laboratory animals for research purposes.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Cell Adhesion: Adherence of cells to surfaces or to other cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Ovum: A mature haploid female germ cell extruded from the OVARY at OVULATION.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Morphogenesis: The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.Integrin alphaVbeta3: An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN).Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Ectoderm: The outer of the three germ layers of an embryo.Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Aorta: The main trunk of the systemic arteries.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Mice, Inbred C57BLDrug Combinations: Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture.Microcirculation: The circulation of the BLOOD through the MICROVASCULAR NETWORK.Matrix Metalloproteinase Inhibitors: Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.Neoplasm Metastasis: The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Embryo, Nonmammalian: The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Lipid Bilayers: Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Urokinase-Type Plasminogen Activator: A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.Cornea: The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.Vascular Endothelial Growth Factor Receptor-1: A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Yolk Sac: The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Endothelium: A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.Mitochondrial Membranes: The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Chemotaxis: The movement of cells or organisms toward or away from a substance in response to its concentration gradient.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Kinetics: The rate dynamics in chemical or physical systems.Synaptic Membranes: Cell membranes associated with synapses. Both presynaptic and postsynaptic membranes are included along with their integral or tightly associated specializations for the release or reception of transmitters.Mice, Inbred BALB COligopeptides: Peptides composed of between two and twelve amino acids.Neoplasm Invasiveness: Ability of neoplasms to infiltrate and actively destroy surrounding tissue.

Inhibitory effects of 9-(4-thio-beta-D-ribo-pentofuranosyl)guanine on tumor growth and angiogenesis. (1/287)

BACKGROUND: To find a nucleoside with anti-angiogenic activity, we tried to screen an active compound from our nucleoside library. MATERIALS AND METHODS: The compound inhibiting the growth of human umbilical vein endothelial cell (HUVEC) induced by the conditioned medium of lung carcinoma cell line PC-9 was screened. The antitumor activity of the compound was evaluated against murine sarcoma S-180 implanted onto chick embryo chorioallantoic membrane (CAM). RESULTS: 9-(4-Thio-beta-D-ribo-pentofuranosyl)guanine (4'-thioguanosine) was found to be a potent inhibitor of the growth of HUVEC. The growth of S-180 implanted onto CAM was also inhibited by 4'-thioguanosine whereas the in vitro growth of S-180 was not inhibited. The administration of 4'-thioguanosine in mice caused unexpected side effect which suggested neurotoxicity. CONCLUSIONS: Antitumor effect of 4'-thioguanosine on S-180 was suggested to be due to inhibition of tumor angiogenesis. Because of toxicity of 4'-thioguanosine in mice, further development of the derivatives which have lower toxicity is required.  (+info)

Proangiogenic action of thyroid hormone is fibroblast growth factor-dependent and is initiated at the cell surface. (2/287)

The effects of thyroid hormone analogues on modulation of angiogenesis have been studied in the chick chorioallantoic membrane model. Generation of new blood vessels from existing vessels was increased 3-fold by either l-thyroxine (T4; 10(-7) mol/L) or 3,5,3'-triiodo-l-thyronine (10(-9) mol/L). T4-agarose reproduced the effects of T4, and tetraiodothyroacetic acid (tetrac) inhibited the effects of both T4 and T4-agarose. Tetrac itself was inactive and is known to block actions of T4 on signal transduction that are initiated at the plasma membrane. T4 and basic fibroblast growth factor (FGF2) were comparably effective as inducers of angiogenesis. Low concentrations of FGF2 combined with submaximal concentrations of T4 produced an additive angiogenic response. Anti-FGF2 inhibited the angiogenic effect of T4. The proangiogenic effects of T4 and FGF2 were blocked by PD 98059, a mitogen-activated protein kinase (MAPK) pathway inhibitor. Endothelial cells (ECV304) treated with T4 or FGF2 for 15 minutes demonstrated activation of MAPK, an effect inhibited by PD 98059 and the protein kinase C inhibitor CGP41251. Reverse transcription-polymerase chain reaction of RNA extracted from endothelial cells treated with T4 revealed increased abundance of FGF2 transcript at 6 to 48 hours, and after 72 hours, the medium of treated cells showed increased FGF2 content, an effect inhibited by PD 98059. Thus, thyroid hormone is shown to be a proangiogenic factor. This action, initiated at the plasma membrane, is MAPK dependent and mediated by FGF2.  (+info)

Effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenoviruses on antiangiogenesis. (3/287)

AIM: To investigate the inhibitory effects of endostatin-vascular endothelial growth inhibitor (VEGI151) recombinant adenoviruses on neovascularization. METHODS: We used recombinant adenoviruses to treat human vascular endothelial cell line ECV304, human hepatocellular carcinoma cell line HepG2, and murine fibroblast cell line L929, in order to study the chimeric gene expression in these cell lines. Chick choriallantic membrane (CAM) model, rabbit inflammatory corneal neovascularization (CNV) model, and liver cancer-bearing nude mice model were employed to investigate the negative biological effect of fusion molecules on neovascularization in vivo. RESULTS: Western blot showed that the molecular weight of fusion protein was about 41 kD after infection of ECV304, HepG2 and L929 cells with supernatant of AdhENDO-VEGI151. The fusion protein showed a specific inhibitory effect on the proliferation of ECV304 cells, but no inhibitory effect on the growth of HepG2 and L929 cells (F=13112.13, P=0.0001). In the chick choriallantic membrane (CAM) assay, the expressed fusion protein significantly inhibited neovascularization. Rabbit inflammatory corneal neovascularization (CNV) induced by intrastromal sutures resulted in a uniform neovascular response. In this model, direct subconjunctival injection of AdhENDO-VEGI151 expressed the fusion protein in vivo and suppressed the development of CNV. Topical application of AdhENDO-VEGI151 led to a significant suppression of CNV (F=1413.11, P=0.0001), as compared with the control group of AdLacZ. Immunohistochemical staining showed the fusion protein dominantly expressed in corneal epithelium. Compared with the control group of AdLacZ (4075.9+/-1849.9 mm(3)), the average tumor size of group AdhENDO-VEGI151 reduced in size (487.7+/-241.2 mm(3)) (F=14.80, P=0.0085), with an inhibition rate of 88.03%. Immunohistochemical staining showed the adenoviruses carried the fusion gene expressed on liver cancer cell membrane. MVD decreased more significantly in treated mice (30.75+/-3.31%) than in AdLacZ control (50.25+/-8.65%) (F=17.72, P=0.0056) with an inhibition rate of 39%. CONCLUSION: Fusion protein expressed by recombinant adenoviruses has a significant inhibitory effect on neovascularization.  (+info)

Regulation of neovascularization by human neutrophil peptides (alpha-defensins): a link between inflammation and angiogenesis. (4/287)

Angiogenesis, the growth of new blood vessels, is a complex biological process that is orchestrated by several growth factors and components of the extracellular matrix, including fibronectin (FN) and its receptor the integrin alpha5beta1. Angiogenesis is a critical part of inflammation and wound repair, but the mechanism by which vascular proliferation and migration is regulated by inflammatory cells is not completely understood. We have previously shown that human neutrophil peptides (HNPs), also known as alpha-defensins, which are secreted in high concentrations when neutrophils are activated, bind specifically to FN in the extracellular matrix and inhibit plasminogen activation. Therefore, we asked whether HNPs act as a link between inflammation and angiogenesis. Alpha5beta1-mediated endothelial cell adhesion and migration to FN, both under control conditions and under stimulation by vascular endothelial growth factor (VEGF), were inhibited specifically and in a dose-dependent manner by HNPs, whereas endothelial cell adhesion and migration to other components of the extracellular matrix, such as vitronectin, collagen, or fibrinogen/fibrin were not. Consistent with this finding, HNPs bound to and promoted the binding of fibronectin to alpha5beta1 integrin in arginine-glycine-aspartic acid (RGD)-independent manner. HNPs also completely inhibited VEGF-induced proliferation and induced apoptosis of endothelial cells in a dose-dependent manner. Moreover, HNPs inhibited capillary tube formation in three-dimensional fibrin-matrices as well as neovascularization in vivo in the chicken chorioallantoic membrane assay. Taken together, these data indicate that HNPs can regulate angiogenesis by affecting endothelial cell adhesion and migration in an FN-dependent manner as well as endothelial cell proliferation. These findings provide new insight into the role of inflammatory cells in angiogenesis and might provide a platform for developing a novel class of anti-angiogenesis drugs.  (+info)

Inhibiting MMP activity prevents the development of endometriosis in the chicken chorioallantoic membrane model. (5/287)

BACKGROUND: Matrix metalloproteinases (MMPs) are essential for extracellular matrix remodelling and may contribute to the development of endometriosis. Transplantation of endometrium onto the chicken chorioallantoic membrane (CAM) results in endometriosis-like lesion formation, a process that requires extensive tissue remodelling. We investigated the expression of a wide range of MMPs in menstrual endometrium, endometriosis-like lesions in CAMs, in peritoneal endometriosis and in endometriosis in the rectovaginal space, as well as the function of MMPs in early lesion formation in the CAM model. METHODS: Expression of MMPs was evaluated by immunohistochemistry and MMP function was studied in the CAM by inhibiting MMP activity during lesion formation. RESULTS: Nearly all MMPs were present in all tissues studied. No significant differences in the expression of a majority of MMPs were found in endometriosis-like lesions in CAMs when compared with human endometriosis. Inhibition of MMP-1, -2, -3, -7 and -13 activities significantly impaired endometriosis-like lesion formation in CAMs. CONCLUSIONS: The MMP expression profiles of experimentally induced endometriosis in CAMs and human endometriosis are similar. The prevention of endometriosis-like lesion formation in the CAM by inhibiting MMP activity strongly suggests that MMPs have a function in the early development of endometriotic lesions.  (+info)

Antiangiogenic activity of semisynthetic biotechnological heparins: low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists. (6/287)

OBJECTIVE: Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives. METHODS AND RESULTS: Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and alpha(v)beta3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/alpha(v)beta3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane. CONCLUSIONS: LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/alpha(v)beta3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.  (+info)

X-rays affect the expression of genes involved in angiogenesis. (7/287)

BACKGROUND: We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that are important regulators of angiogenesis: vascular endothelial growth factor (VEGF), heparin affin regulatory peptide (HARP) and inducible nitric oxide synthase (iNOS). MATERIALS AND METHODS: An area of 1 cm2 of the CAM, restricted by a plastic ring was irradiated at room temperature. The expression of the genes was studied using RT-PCR and the amounts of the mRNAs were quantified using image analysis of the corresponding agarose gels of the RT-PCR products. RESULTS: VEGF mRNA was decreased 6 h after irradiation. However, at later time points, VEGF expression was significantly increased compared with the nonirradiated tissue. Similarly, X-rays down-regulated both HARP and iNOS expression 6 h after irradiation and the effect was reversed at later time points, similarly to the effect of X-rays on VEGF. CONCLUSION: These data support the notion that X-rays increase the expression of genes that favor angiogenesis.  (+info)

Angiogenic response induced by acellular aortic matrix in vivo. (8/287)

In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a well-known angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-beta1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-beta1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-beta1 in the acellular matrices.  (+info)

*Chorioallantoic membrane

The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of ... with air found in pores of the shell membrane of the egg. As a result, the chorioallantoic membrane allows exchange of gases, ... Chorioallantoic membranes from developing chicken eggs are routinely used in biological and biomedical research to investigate ... Ribatti D (2008). "Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis". Int Rev Cell Mol Biol. 270: ...

*Withanolide

Withaferin A also inhibits angiogenesis on chick chorioallantoic membrane. Withanolides inhibit COX-2 and exhibit anti- ...

*Ex vivo

One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted ... on the CAM membrane of a chicken embryo outside the organism (chicken). Animal testing In situ In utero In vitro In vivo In ...

*Placentation

... the intimate interaction of the uterus and a series of embryonic membranes including the chorioallantoic and yolk sac membranes ... "Comparative genomics of hormonal signaling in the chorioallantoic membrane of oviparous and viviparous amniotes". Gen Comp ... The placenta can be defined as an organ formed by the sustained apposition or fusion of fetal membranes and parental tissue for ... communication occurs via the production of a range of signalling molecules and their receptors in the chorioallantoic membrane ...

*Virus

The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos. Science. 1931;74(1919):371-372. ... either the outer membrane surrounding an infected host cell or internal membranes such as nuclear membrane or endoplasmic ... This membrane is studded with proteins coded for by the viral genome and host genome; the lipid membrane itself and any ... Viral enzymes aid in the breakdown of the cell membrane, and, in the case of the T4 phage, in just over twenty minutes after ...

*History of virology

"The growth in vitro of vaccinia virus in chick embryo chorio-allantoic membrane, minced embryo and cell suspensions". The ... "The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos". Science. 74 (1919): 371-372. ...

*Introduction to viruses

"The cultivation of vaccine and other viruses in the chorioallantoic membrane of chick embryos". Science. 74 (1919): 371-372. ... The causes of death include cell lysis (bursting), alterations to the cell's surface membrane and apoptosis (cell "suicide"). ...

*SULF1

In contrast to Sulf1, Sulf2 actually induced angiogenesis in a chick chorioallantoic membrane assay. Sulf2 was measured for its ... Vascular basement membrane (BM) HSPGs are modified to bind L-selectin and monocyte chemoattractant protein-1 (MCP-1) during ... In addition to cell membrane association, Sulfs also secreted freely into the media, which contrasts the findings with QSulf1 ... QSulf1 localizes exclusively to the cell surface by interacting hydrophilically with a non-heparan sulfate outer membrane ...

*In ovo

During the incubation period, the virus replicates in the cells that make up the chorioallantoic membrane. In human vaccine ...

*Vascular endothelial growth factor C

... specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane". Developmental ...

*Chorion

... the chorion and the allantois fuse together to form the chorioallantoic membrane. Together these form a double membrane, which ... In reptiles, birds, and monotremes, the chorion is one of the four extraembryonic membranes that make up the amniotic egg that ... It is formed by extraembryonic mesoderm and the two layers of trophoblast that surround the embryo and other membranes. The ... For the entertainment company see Chorion (company). The chorion is the outermost fetal membrane around the embryo in mammals, ...

*Michael Jeltsch

... specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane". Developmental ...

*Proteases in angiogenesis

... bFGF induced vascularization in the corneal pocket assay and inhibit VEGF induced angiogenesis in the chorioallantoic membrane ... Shedding of a membrane anchored cytokine or growth factor by ADAM proteinases may be relevant for various signal transduction ... Semaphorin 4D is cleaved from its membrane bound form by MT1-MMP (MMP-14) in tumor cells; it then interacts with plexin B1 on ... The vascular basement membrane is composed of type IV collagen, type XV collagen, type XVIII collagen, laminins, entactin, ...

*Raccoonpox virus

When inoculated on the chorioallantoic membrane (CAM) of 12-day embryonated hens' eggs, the RCN produced many small discrete ... Genome replication begins with the uncoating of the virus core as it comes into contact with the plasma membrane. Once uncoated ...

*Influenza vaccine

... past the chorioallantoic membrane. The two holes are then sealed with melted paraffin and the inoculated eggs are incubated for ...

*Cucurbitacin E

... property of cucurbitacin E was demonstrated in vitro but also in vivo in a chick embryo chorioallantoic membrane and in a mouse ...

*Chicken as biological research model

The chorioallantoic membrane (CAM), a well-vascularized extra-embryonic tissue located underneath the eggshell, has a ... Woodruff, A. M.; Goodpasture, E. W. (1931). "The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection ...

*Mary Logan Reddick

... when transplanted to the chorioallantoic membrane of a chick in a later stage of development, in "cut-and-paste" experiments. ... Reddick ML (1937). The differentiation of portions of the chick blastoderm in chorio-allantoic grafts. Thesis for Degree of the ... "The differentiation of portions of the chick blastoderm in chorio-allantoic grafts". Robert W. Woodruff Library. University of ... Reddick, Mary Logan (June 1945). "The differentiation of embryonic chick medulla in chorioallantoic grafts". The Journal of ...

*Interferon

... with a system that involved the inhibition of the growth of live influenza virus in chicken embryo chorioallantoic membranes by ... such as membrane bound Toll like receptors or the cytoplasmic receptors RIG-I or MDA5, can trigger release of IFNs. Toll Like ... that this interference was mediated by a protein released by cells in the heat-inactivated influenza virus-treated membranes. ...

*Alice Miles Woodruff

Woodruff, Alice Miles; Goodpasture, Ernest W. (May 1931). "The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos ...

*List of MeSH codes (A16)

... chorioallantoic membrane MeSH A16.254.300.600 --- cleavage stage, ovum MeSH A16.254.300.600.274 --- blastula MeSH A16.254. ... vitelline membrane MeSH A16.631.900 --- zona pellucida MeSH A16.759.189 --- chorionic villi MeSH A16.759.289 --- decidua MeSH ... 300.600.550 --- morula MeSH A16.254.300.800 --- yolk sac MeSH A16.254.403 --- extraembryonic membranes MeSH A16.254.403.147 ...

*Draize test

... egg chorioallantoic membrane (HET-CAM) assay and an epithelial model cultivated from human corneal cells, in comparison with ... "Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes". J. ...

*Index of oncology articles

... chorioallantoic membrane - choriocarcinoma - choroid plexus tumor - CHPP - chronic granulocytic leukemia - chronic idiopathic ... synovial membrane - synovial sarcoma - synthetic protegrin analog - synthetic retinoid - systemic chemotherapy - systemic ... mesenteric membrane - mesna - mesonephroma - mesothelioma - metaplasia - metaplastic carcinoma - metastasectomy - metastasis - ...

*Cam (disambiguation)

... proteins on cell surfaces Chorioallantoic membrane, in developing eggs Crassulacean acid metabolism, a plant carbon-fixing ...

*List of MeSH codes (A13)

File "2006 MeSH Trees".) MeSH A13.350.150 --- chick embryo MeSH A13.350.575 --- chorioallantoic membrane MeSH A13.395.248 --- ...

*Membrane (disambiguation)

... a membrane that separates the interior of all cells from the outside environment Chorioallantoic membrane, a vascular membrane ... Membrane may also refer to: Biology: Biological membrane, including: Amnion, a membrane in the amniotic sac Basement membrane, ... the biological membrane of an organelle or Gram-negative bacteria that is within an outer membrane Mucous membrane, linings of ... a smooth membrane consisting of a thin layer of cells, which secrete serous fluid Tunic membrane, protective membrane covering ...
TY - JOUR. T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. AU - Mangieri, Domenica. AU - Nico, Beatrice. AU - Benagiano, Vincenzo. AU - De Giorgis, Michela. AU - Vacca, Angelo. AU - Ribatti, Domenico. PY - 2008/6. Y1 - 2008/6. N2 - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve ...
The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes, such as birds and reptiles. It is formed by the fusion of the mesodermal layers of two developmental structures: the allantois and the chorion.[1] In mammals, this structure forms the placenta. Three different layers compose the chorioallantoic membrane; these are called the chorionic epithelium, the mesenchyme and the allantoic epithelium. Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen, to developing embryos.[1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell.[1] Chorioallantoic membranes from developing ...
The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay
Angiogenic potential of the cerebrospinal fluid (CSF) of patients with high-grade gliomas measured with the chick embryo chorioallantoic membrane assay (CAM) ...
537 The expression of matrix metalloproteinases (MMPs) plays crucial roles in cancer progression and metastasis. This study is to clarify whether MMP associates with radiation-accelerated pulmonary metastasis of Lewis lung carcinoma (LLC-LM) in C57BL/6 mouse model. By using in vitro matrigel-coated Boyden chamber invasion assay, non-cytotoxic dose of radiation (7.5 Gy) significantly enhanced LLC-LM cell invasiveness. Irradiated LLC-LM cells showed the increased intravasation capability in the chicken chorioallantoic membrane assay. To test MMP family protein gene expression in irradiated LLC-LM cells, MMP-9 gene expression was significantly enhanced in both transcriptional and translational levels, so was the enzyme activity in the culture supernatant. The use of anti-sense MMP-9 oligonucleotides significantly inhibited the in vitro radiation-enhanced invasiveness. With 1 x 106 MMP-9 RNAi stably transfected LLC-LM cells injected subcutaneously in the right thighs of C57BL/6 mice and irradiated ...
The overall objectives of this thesis were to develop a short-term culture system and to examine the effects of vitrification and short-term culture on the viability of fresh and vitrified bovine ovarian tissue and the follicles within. The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. Bovine ovaries were retrieved from a local abattoir and cortical pieces (1-2mm3) were randomly assigned to one of the following groups; control (fixed immediately), CAM or in in vitro culture. Ovarian tissue fragments from both groups were removed on D1, D3 and D5 of culture, fixed, sectioned (5μm) and stained with H&E. The numbers of healthy and ...
Shark (Sinica cetorhinus maximum) cartilage was extracted in 1 mol/L Gu-HCl guanidine. Two purified active proteins with apparent molecular weights of 15.2x103 Da and 8.0×103 Da (designated as Sp15 and Sp8, respectively) were obtained through ultrafiltration and Superdex 75 chromatography. The activities of the samples were studied in terms of their potential inhibition of vascular endothelial cell growth in vitro, of angiogenesis both in rabbit cornea and chick embryo chorioallantoic membrane (CAM) assay models in vivo, and of growth of transplanted S180 sarcoma in mice in vivo. The results showed that Sp15 expressed a typical lysozymatic activity up to 223,000 U/mg and its N-terminus was highly homologous to lysozymes of various mammalian origins. Sp15 exhibited a strong anti-angiogenic activity only in vitro, whereas Sp8 shared this effect both in vitro and in vivo. Both Sp15 and Sp8 provided an effective anti-tumor activity in mice bearing transplanted S180 sarcoma. These results suggest that Sp15
The aims of this work are to study the antitumor effect of the adeno-associated virus on the xenografted tumors of chick embryo chorioallantoic membrane and predict potential genes and biological pathways which are associated with renal cell carcinoma. The adeno-associated virus NT4-TAT-6 × His-VHLbeta was constructed and identified. Then, chick embryos with xenografted tumor were divided into three groups and respectively inoculated with rAAV/NT4-TAT-6 × His-VHLbeta (group A), empty virus (group B), and phosphate-buffered saline (group C, the control subject). Antitumor effect in each group was investigated by means of immunofluorescence observation. Genes interacted with von Hippel-Lindau were screened by Search Tool for the Retrieval of Interacting Genes/Proteins database, while pathway analysis were performed based on Kyoto Encyclopedia of Genes and Genomes. The growth of xenografted tumors inoculated with recombinant adeno-associated virus was slower than the control subjects. The tumor ...
Introduction: The membrane-bound metalloproteinase aminopeptidase N (APN) is involved in several different cellular processes associated with the malignant phenotype. Furthermore APN has also been reported to have a functional role in tumor angiogenesis and there appear to be a strong correlation between the expression of APN and the invasive capacity of a numerous tumor cell types. The melphalan-derived prodrug melphalan-flufenamide (previously designated J1) can be activated by APN suggesting possible anti-angiogenic properties.. Experimental: A series of experiments were performed to evaluate the effects of melphalan-flufenamide on angiogenesis; cytotoxicity on bovine endothelial cells, the TCS Cellworks AngioKit model with human endothelial cells co-cultured with fibroblasts, the chicken embryo chorioallantoic membrane assay, and finally in vivo in mice using the Cultrex DIVAA angio-reactor assay.. Results: Melphalan-flufenamide displayed high cytotoxic activity against endothelial cells, ...
When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. The number of foci is positively correlated with the number of cells in the inoculum. The phenomenon is known to be an expression of an immunological reaction of the grafted cells against the host. F. M. Burnet and his colleagues have suggested that each focus results from the immunological activity of one and only one cell. If this presumption were confirmed the CAM system would gain in usefulness, in particular for the isolation of clones of immunologically competent cells. In the present work this single-hit hypothesis has been subjected to a specific test of its biometrical consequences. On the single-hit model the response should be proportional to the dose. Equivalently, on the logarithmic scale of measurement adopted in these experiments, the fitted dose-response line should show a ...
The authors studied the specific tumor antigen in Crockers sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing...
One of the major problems in assessing neovascularization in mammalian experimental animal models is the immunologic response of the host to stimuli from nonautologous species. Hence, crude bovine vitreous and retinal extracts may produce a complex immune reaction when tested in the rabbit. To circumvent this problem, the chicken chorioallantoic membrane (CAM) assay is most appropriate. In this study the CAM assay for angiogenesis has been modified to study antiangiogenic substances. The modified assay is described in detail and used to demonstrate for the first time the inhibition by adult bovine vitreous of neovascularization induced by extracts of adult bovine retina. In addition to vitreous, three common glycosaminoglycans (keratan sulfate, chondroitin sulfate C, and hyaluronic acid) were assayed for antiangiogenic activity. The results indicate that vitreous inhibition of retinal extract-induced neovascularization is dose dependent, while the sulfated glycosaminoglycans tested had no ...
The long‐term survival and successful integration of implants for tissue replacement and regeneration highly depends upon the fast ingrowth of blood vessels from the surrounding tissues
MT Pinto joined Ipatimup as an Investigator Ciência 2008 (2009) of the Cancer Genetics Group and was mainly dedicated to the study of cancer related features using the CAM model. After setting up the lab and implementing several CAM assays, she established the "In vivo CAM assays service" at Ipatimup (2012) and founded the spin-off "Expertus, CAM assays" (2013-2015). MT Pinto graduated in Biochemistry (FCUP; 1999) and both MSc (FMUL; 2001) and PhD (FMUP; 2008) theses concerned the study of the endogenous pain control system in acute and chronic pain states and involved extensive use of animal models. MT Pinto has published 26 papers in international peer reviewed journals (Q1) with an H index of 12 (ResearcherID: J-2519-2013). She is co-author of two patents. ...
Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis. Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent. Results showed that TF-SB
Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Figure 6: |i|Viola tricolor|/i| Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane
TY - JOUR. T1 - Hydrogen sulphide induces angiogenesis. AU - Pyriochou, Anastasia. AU - Marazioti, Antonia. AU - Szabo, Csaba. AU - Papapetropoulos, Andreas. PY - 2009. Y1 - 2009. N2 - Hydrogen sulphide (H2S) is emerging as an important signalling molecule in the cardiovascular and neuronal systems. Although H2S is known to relax vascular smooth muscle leading to a reduction in blood pressure, the role of this gaseous mediator in other aspects of the biology of vascular cells remains largely unexplored. In order to study the effects of H2S on neovascularisation, we have utilised an aqueous formulation of H2S. Incubation of endothelial cells (EC) with H2S enhanced their angiogenic potential as it promoted cell growth, migration and capillary morphogenesis on matrigel. In line with the in vitro observations, treatment of chicken chorioallantoic membranes (CAM) with H2S increased vascular length. Morever, exposure of EC to H2S resulted in increased phosphorylation of kinases linked to angiogenic ...
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number of human diseases. In vivo neovascularization assays that reflect physiological and pathological formation of neovessels are important in this effort. In this report we present an assay where the effects of activators and inhibitors of angiogenesis can be quantitatively and qualitatively measured. A provisional matrix composed of collagen I and fibrin was formed in a plastic cylinder and implanted onto the chick chorioallantoic membrane. A nylon mesh separated the implanted matrix from the underlying tissue to distinguish new from pre-existing vessels. Vascularization of the matrix in response to fibroblast growth factor-2 or platelet-derived growth factor-BB was scored in a double-blinded manner, or vessel density was measured using a semi-automated image analysis procedure. Thalidomide, fumagillin, U0126 and TGFβ inhibited neovessel growth while hydrocortisone exerted a negative and ...
ABSTRACT: 15 K is 1,2, 3-triazolyl ester of ketorolac, an old pain-killer, that blocks PAK1 by its R-form and inhibits COX-2 by its S-form. Mainly due to a robust increase in cell-permeability, 15K is over 500 times more potent than ketorolac in both anti-cancer and anti-PAK1 activities in cell culture with IC50 around 24 nM. However, 15K has no anti-AKT activity. Angiogenesis requires at least the kinase PAK1, and perhaps the kinase AKT as well, and is essential for a robust growth of solid tumors. Thus, in this study, we examined the potential antiangiogenic activity of 15K both in ovo and cell culture, prior to its in vivo (xenograft) anti-cancer activity test. The IC50 of 15K against the embryonic angiogenesis in ovo in CAM (chorioallantoic membrane) assay is around 1 nmol/egg. Surprizingly, however, 15K failed to inhibit the tube formation of HUVECs (human umbilical vein endothelial cells) in cell culture even at high as 150 μM. In an attempt to solve this mystery, we tested both in ovo as ...
Glaucium flavum (G. flavum) is a plant from the Papaveraceae family native to Algeria where it is used in local traditional medicine to treat warts. G. flavum root crude alkaloid extract inhibited breast cancer cell proliferation and induced G2/M phase cycle arrest and apoptosis without affecting normal cells, which is a highly awaited feature of potential anti-cancer agents. G. flavum significantly reduced growth and vascularization of human glioma tumors on chicken chorioallantoic membrane (CAM) in vivo. The chromatographic profile of the dichloromethane extract of G. flavum root showed the presence of different constituents including the isoquinoline alkaloid protopine, as the major compound. We report for the first time that G. flavum extract may represent a new promising agent for cancer chemotherapy.
Anticancer Effect of COX-2 Inhibitor DuP-697 Alone and in Combination with Tyrosine Kinase Inhibitor (E7080) on Colon Cancer Cell Lines Colon cancer;DuP-697;COX-2 inhibition;tyrosine kinase inhibitor; Colorectal cancer remains one of the most common types of cancer and a leading cause of cancer death worldwide. In this study, we aimed to investigate effects of DuP-697, an irreversible selective inhibitor of COX-2 on colorectal cancer cells alone and in combination with a promising new multi-targeted kinase inhibitor E7080. The HT29 colorectal cancer cell line was used. Real time cell analysis (xCELLigence system) was conducted to determine effects on colorectal cell proliferation, angiogenesis was assessed with a chorioallantoic membrane model and apoptosis was determined with annexin V staining. We found that DuP-697 alone exerted antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. For the antiproliferative effect the half maximum inhibition concentration ($IC_{50
TY - JOUR. T1 - Wogonin inhibits IL-6-induced angiogenesis via down-regulation of VEGF and VEGFR-1, not VEGFR-2. AU - Lin, Chiu Mei. AU - Chang, Hang. AU - Chen, Yeh Hsu. AU - Wu, I. Hsing. AU - Chiu, Jen Hwey. PY - 2006/11. Y1 - 2006/11. N2 - So far, no antiangiogenic activity of wogonin, a flavonoid, on human umbilical vein endothelial cells (HUVECs) has been demonstrated. The aim of this study was to investigate the effects of wogonin on IL-6-induced angiogenesis in HUVEC cultures and chorioallantoic membrane (CAM) neovascularization. The in vivo CAM model was applied to evaluate the percentage of new vessel formations, followed by measurement of endothelial migration and tube formation in HUVEC cultures. The results revealed that wogonin (10-8-10-5M) concentration-dependently inhibited IL-6-induced angiogenesis. The signaling pathway was through down-regulation of the autocrine loop of VEGF and VEGFR-1, but not of VEGFR-2. Furthermore, the regulating loop of the IL-6 receptor complex was ...
Authors: Riordan NH, Meng X, Riordan HD.. ABSTRACT: Recruitment of new blood vessels plays a crucial role in tumor survival and growth. Several agents that act as angiogenesis inhibitors are currently being investigated as anti-tumor agents. Proteoglycan extract (PGM) was tested for anti-angiogenic, immunostimulatory, and anti-neoplastic activity. PGM is a non-toxic extract of the ubiquitous plant, Convolvulus arvensis. In the chicken egg chorioallantoic membrane assay PGM inhibited new blood vessel growth in a dose-dependent manner. Results were 18, 55, and 73% inhibition at concentrations of 50, 100, and 200 mcg, respectively. PGM significantly inhibited tumor growth in the mouse fibrosarcoma (S-180 Kun Ming 3-4 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days), and mouse Lewis lung carcinoma (C57, 6 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days) models. Inhibition (54-77% inhibition by weight compared to controls, up ...
A swept source based Fourier domain optical Doppler tomography (FDODT) system was developed. The technique is based on a phase-resolved method where phase information was retrieved from the reconstructed complex fringe signals. The aliasing effects and artifacts caused by lateral scanning and sample movement were removed with a signal processing technique. The standard deviation of the phase shift of the system was reduced from 49 to 1.8 degrees with the signal processing method employed. Structural, Doppler and Doppler variance images of fluid flow through glass channels were quantified, and blood flow through vessels of chick chorioallantoic membrane (CAM) was demonstrated in vivo.. ©2005 Optical Society of America. Full Article , PDF Article ...
J:31267 Spindle A, Sturm KS, Flannery M, Meneses JJ, Wu K, Pedersen RA, Defective chorioallantoic fusion in mid-gestation lethality of parthenogenone
Rigorous and systematic pre-clinical studies are necessary and essential to establish the efficacy and safety of Oriental herbs and formulas in order to transform traditional herbal practices into evidence-based medicine. Here we evaluated the anti-cancer activities of the ethanol extract of Ka-mi-kae-kyuk-tang (KMKKT), a formula of ten Oriental herbs, with a battery of in vitro and in vivo mechanism-based biomarkers involving angiogenesis, apoptosis and metastasis. The results show that KMKKT suppressed the vascular endothelial responses by inhibiting basic fibroblast growth factor (bFGF)-induced ERK1/2 phosphorylation, cell migration as well as tube formation in the human umbilical vein endothelial cell model, and decreased the hypoxia-induced HIF1α and vascular epithelial growth factor (VEGF) expression in the mouse Lewis lung carcinoma (LLC) cells in vitro, and inhibited the bFGF-induced angiogenesis in chick chorioallantoic membrane model, and in the Matrigel plugs in mice. Intraperitoneal ...
Definition of chorioallantoic placenta. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Shikonin has anticancer activity, but it has not yet been applied into clinical use. In the present study, shikonin was prepared using liposomes. We aimed to examine several aspects of sh-L (shikonin-containing liposomes): preparation, angiogenic suppression and cellular uptake through self-fluorescence. Sh-L were prepared using soybean phospholipid and cholesterol to form the membrane and shikonin was encapsulated into the phospholipid membrane. Three liposomes were prepared with shikonin. They had red fluorescence and were analysed using a flow cytometer. Angiogenic suppression of sh-L was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide], Transwell tests, chick CAM (chorioallantoic membrane) and Matrigel™ plug assay. MTT assay showed the median IC50 (inhibitory concentrations) as follows: shikonin, sh-L1 and sh-L2 were 4.99±0.23, 5.81±0.57 and 7.17±0.69 μM, respectively. The inhibition rates of migration were 53.58±7.05, 46.56±4.36 and ...
Background. Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive solid cancers with a 5-year survival rate approaching a dismal 5%. New therapeutic targets need to be identified in order to increase survival rates.. HOX genes are a family of 39 transcription factors involved in embryonic development and which have been found to be dys-regulated in PDAC. HOX gene function can be antagonised by HXR9, a peptide that competitively inhibits binding of HOX proteins to their co-factor PBX. We established that the CAM model recapitulates human PDAC, including desmoplasia, and is highly suitable for the evaluation of HXR9 in vivo.. Experimental procedure. Four PDAC-derived cell lines (AsPc-1, BxPc-3, PaCa-3 and PancTu1) were treated in vitro with HXR9. Ic50s were determined using both MTS and LDH assays. FACS analysis was used to determine mechanisms of cell death. Cell lines were subsequently implanted in the CAM using Marigel™ on embryonic day (EDD) 10. Tumour growth curves were ...
If you dont agree with the NSA surveillance, but youre not concerned because it does not personally impact you, thats a point worth exploring.
In chick chorioallantoic membrane (CAM) assays, injection of cilengitide into CAM vasculature prevented the growth of implanted U87MG glioma and DAOY medulloblastoma tumor fragments compared to the inactive peptide EMD135981. The inhibition of tumor growth was attributed to the anti-angiogenic activity of cilengitide as membrane vascularity surrounding the tumor graft was greatly decreased by cilengitide treatment as compared to the inactive control peptide [130]. In animal models, cilengitide, as a single agent effectively inhibited the growth and proliferation of orthotopically implanted malignant glioma and medulloblastoma cell lines in nude mice. Daily treatment of established xenografts with 100-200 µg cilengitide significantly delayed tumor growth and prolonged survival compared to controls. The delayed tumor growth was accompanied by increases in CD31 and tumor doubling times, elevated apoptotic index and decreases in proliferative index and tumor vessel number and density [130]. ...
Abstract: Often the fields of tissue engineering and cancer research are studied in isolation, inhibiting cross-talk and collaboration between cancer researchers and tissue engineers. This work has aimed at bridging these fields in a way that provides insight into the clinical problems of breast cancer and bone metastasis. The research discussed herein focuses on how host cells contribute to the g... read morerowth and progression of breast cancer during bone metastasis. Preliminary studies used the chick CAM, chorioallantoic membrane, to investigate angiogenesis and bone metastasis (Chapter 2). Then mouse models and tissue-engineered bone were utilized to study mesenchymal stem cell (MSC) tumor homing and the effects of MSCs on cancer progression (Chapter 3). Results suggested that tumor-derived TGF-beta1 (transforming growth factor-beta1) is likely to play a large role in attracting MSCs to breast tumors in patients. MSC-derived IL-17B (interleukin 17B) was found to stimulate breast cancer ...
The NSA has done one hell-ave job in China and the rest of Asia. This is not talked about much, the exact number is not known, but it has been stated that up to 100,000 business in China are owned by Tawian/NSA.. Almost all major U.S. companies have NSA members on the Boards of Directors. Most committees that report to congress also have members. Most presidents have also been members of the NSA. We know this because most presidents have been on the Media committee that controls the Media data that can be harmful to the public. I am not saying this is bad at all. Many of the NSA members are specialist in their field of education. The idea seems to be that the NSA uses top professors and people to help make decisions that effect the U.S. and the world. They work for our major colleges and universities. Working for the NSA is task driven. Finish the operation and wait for the next call. It is not like a full time job. Some companies that are NSA owned and operated are managed so well that the ...
When online you basically keep broadcasting, here is where I am, send me this data, information that is meant to be available to the server/servers you are connected to. They generally just give out where your from, what your network connection is like, what browser you use, port number, internet protocol etc. This information is meant to be used for running FTP and HTTP operations making ISP doorways where they who goes in and who goes out. Now with the NARUS semantic network scanning that the NSA is supposed to be using they not only know who goes out and who goes in they also know where their going, what theyre talking about and whom theyre talking too! The NSA could do this before too mind you, but this is the first time we know that they are doing this en-masse. Plus this is data directly sourced form the ISP meaning that even those connections routed through the AT&T server would go the NSA "spybox ...
As new details emerge on NSA leaker Edward Snowden and his girlfriend, MSNBCs Lawrence ODonnell debates the NSA program with MSNBCs Chris Hayes and Alex Wagner.
Jewel v. NSA, EFFs landmark case challenging NSAs mass spying moved forward in 2019, setting up a crucial decision for the Ninth Circuit in 2020. Weve pursued this case for over a decade because we believe that mass surveillance, like all general search and seizure schemes, is both illegal and...
The planned NSA data center facility in Camp Williams in Utah will span 1 million square feet, with about 100,000 square feet dedicated to raised floor data center space.
The feasibility of photoacoustic microscopy (PAM) for evaluation of angiogenesis inhibitor was investigated on a chick embryo model in vivo. Different concentrations of the angiogenesis inhibitor, Sunitinib, were applied to the chorioallantoic membrane (CAM) of the chick embryos. Imaging of microvasculature in embryo CAMs was acquired using a laser-scanning PAM system; while the optical microscopy (OM) capturing the microvascular images of the same set of CAMs for comparison served as a gold standard for validating the results from PAM. The microvascular density as a function of applied Sunitinib concentration has been quantified in both PAM and OM images. The results from these two modalities have a good agreement, suggesting that PAM could provide an unbiased quantification of microvascular density for objective evaluation of anti-angiogenesis medication. In comparison with conventional OM which enables only two-dimensional enface imaging, PAM is capable of three-dimensional analysis of ...
Unable to get any more film offers, she is forced to work as a nude model for cheap girlie magazines. Watch this video free on GotPorn. Check out the hottest Asian Cam Models in the web: hotties fuck and. Check this site for best pics of korean nude models Asian beauties, japan teen models, Korean pussy pictures, free Chinese porn, Asian school girls, Japanese amateurs! Jul 20, 2017, Asian Nude Pageant Model from San Francisco. Jiho Lee is a South Korean model and fitness instructor, who's best known for his. If you find Korean models very attractive, then you must know the 5 best Korean nude models. Korean girl group members look like models, and have. Nude Girls Beach Voyeur Hidden Cam. And not forgetting those nude high cut boots, an adventurous option. If you like photos of beautiful Korean ladies showing their naked bodies, this is the right place for you. Korean-American Nude Model Kyla Gray Leaked Nude Photos. But most of the paintings of female nudes were portraits of models ...
Premiered at Gamescom last year, Ubisofts Oculus Rift send-up, the fart-simulating Nosulus Rift, gives gamers the ability to smell the farts of characters from the second South Park game, appropriately named The Fractured but Whole (Dont get it? Try reading it aloud).. "The Nosulus Rift is a fully functional mask using sensors activated through inaudible sound waves in the in-game fart sound, every time the player makes use of his nefarious [fart] powers," an Ubisoft spokesperson told us. "Each time the sensors are activated, they trigger the odours puff. Meticulously and without mercy.". Virtual flatulence not your bag? How about virtual body odour, breath, or even private parts? Earlier this year, adult entertainment webcam platform CamSoda announced a device called the OhRoma; a gasmask-style wearable with interchangeable odour canisters that releases smells matching any of the thirty broadcast by a cam model via Bluetooth. The company is taking pre-orders now.. Both of these nasal ...
Jewett waves brachial autonomic plexus expiration lateral aspect bacterial encephalitis chorioallantoic Cheyne-Stokes psychosis dofn extrinsic color inferior articular process lamina suprachoroidea choroidea kinetochore fibers CT scan Aerococcus lateral nasal process ...
TMB Blotting PLUS™ for colorimetric detection in membrane assays based on Horseradish Peroxidase conjugates. Reactions develop a stable, dark blue color.
Practical applications: Unsaturated lactones are structural elements often found in natural products, which have medicinal value. Benzolactones derived from anacardic acid reported in this work have some structural similarity with lactones such as massoia lactone having medicinal value. Therefore with this idea in mind, the unsaturated benzolactones reported in this work will be tested for their anti pathogenic activity. 3-Propylphenol is used in combination with racemic 1-octen-3-ol and p-cresol to prepare a kairomone for tsetse fly traps. Results from this work describe the suitability of anacardic acid for synthesizing 3-propylphenol. The fact that 3-propylphenol can be synthesized from anacardic acid, a component of cashew nut shell liquid is of particular interest since most of the areas affected with tsetse flies are suitable for growing cashew plants. This means the raw materials (CNSL) for synthesis of 3-propylphenol will be obtained from within the same region where the kairomone is to ...
Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo Marta Grodzik1, Ewa Sawosz1, Mateusz Wierzbicki1, Piotr Orlowski1, Anna Hotowy2, Tomasz Niemiec1, Maciej Szmidt3, Katarzyna Mitura4, André Chwalibog21Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Basic Animal and Veterinary Science, University of Copenhagen, Copenhagen, Denmark; 3Division of Histology and Embryology, Warsaw University of Life Sciences, Warsaw, Poland; 4Department of Biomedical Engineering, Koszalin University of Technology, Koszalin, PolandAbstract: The objective of the study was to determine the effect of carbon nanoparticles produced by different methods on the growth of brain tumor and the development of blood vessels. Glioblastoma multiforme cells were cultured on the chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor.
Presented in Symposium I (Joseph Holson, L.B. Pearce, co-chairs) at the Forty-Third Annual Meeting of the Teratology Society, Philadelphia, June 26, 2003.
Myeloid-derived suppressor cells (MDSC) certainly are a main element of the immune system suppressive network defined in cancer and several additional pathological conditions. tumor-free mice. Manifestation of NOX2 subunits in MDSC was managed by the STAT3 transcription element. In the lack of NOX2 activity MDSC dropped the capability to suppress T-cell reactions and quickly differentiated Read More. ...
That is supposition on your behalf. The only person who knows what Ed Snowden liberated from the NSA archive systems is in all probability Ed and that only in a general sense. Likewise what was actualy distributed is known only to Ed and those he gave them to either for use or safe keeping. Those he gave to Glen Greenwald we indirectly know --from others Glen showed them to initially-- exceed those that have so far been published. Realistically we also know that of those they wont all get published in newspapers as they are lossing their news worthyness as time goes by. After a decade most will have lost any interest to the public at large, and be only of interest to the techno curious and historians, unless of course they have real blackmail potential for seniors in the US IC.... However if we are to believe what the NSA, UK IC and others variously claimed its between 50,000 and 1.7million individual documents, which suggests both numbers are complete guesses[0].. The high figure fails the ...
My best presentation blog posts, reorganized, fixed, prettier. Buy the Kindle ebook on Amazon, or get the free PDF on my home page. ...
An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the peptide cotransmitter, somatostatin. The present study tests the hypothesis that differences in the level of expression of activin A and its endogenous inhibitor follistatin in CG neuron target tissues are responsible for selective expression of somatostatin in choroid neurons. Intraocular injection of activin A or follistatin (300 ng injected at E10/E11) in cultured embryos resulted in a 39% increase or a 23% decrease, respectively, in somatostatin-positive neurons relative to controls. Chorioallantoic membrane application of follistatin (1 microgram daily from E7 to E13) reduced somatostatin positive neurons by 54%. Neuron number, size, and target tissue morphology were unaffected ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries. ...
Comparing volumetric and biochemical assessment of intravasation caused by hysteroscopic surgery Oscar HaudeLucilla E. OverdijkPaul J.M. van KesterenBart F.
InformationWeek.com: News, analysis and research for business technology professionals, plus peer-to-peer knowledge sharing. Engage with our community.
Because I seem to know what the leaders of Iraq dont--the identity and whereabouts, at least for part of last year, of the supposedly most-wanted terror...
Infection of humans with the zoonotic bacterium Bartonella henselae (Bh) can result in a range of clinical symptoms and disease including lymphadenopathy associ...
Poster (2011, January 31). DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA ... [more ▼]. DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA methyltransferases (DNMT) inhibitors are potent anti-angiogenic compounds. Though combination of HDAC and DNMT inhibitors are now being examined in clinical trials of hematological malignancies, little work has been done to understand the effect of this combination on physiological and tumoral angiogenesis. We have designed and tested a family of twin drugs with intrinsic HDAC and DNMT inhibitory activities in relevant models of angiogenesis in vitro (Human Umbilical Vein Endothelial Cells - HUVEC and aortic ring) and in vivo (chick chorioallantoic ...
Various modifications to a porous substrate, such as employed in lateral flow assay devices, to regulate or modify the flow rate and/or flow path pattern of a fluid through the porous substrate is described. The lateral flow assay device has a porous substrate matrix in fluid communication with a flow-rate control zone having a number of flow-rate control devices arranged as features in or on a substrate surface or laminates thereof in a body. The flow-rate control devices may include: a density gradient, porosity gradient, ion affinity gradient, micro-channels, and combinations thereof.
For a member of the skink family, blue-tongued skinks are among the best suited for lives in captivity for several reasons. Being relatively slow-moving, theyre not as difficult to handle compared to most of the slender skink species. Blue-tongued skinks short, thick physique makes them easy to hold, even by children. Although a biter is occasionally encountered, most adult blue-tongued skinks are loathe to even open their mouths, much less show off their startlingly fat, blue tongues that earned them their common name.. A 20-gallon long aquarium should be considered minimal housing for one specimen. Nearly any substrate from sand to pebbles to aspen provides blue-tongued skinks an adequate material to wander about in. A simple shelter of stone or a concave piece of wood will serve as a hide box. Blue-tongued skinks should have a small bowl of water available at all times. They may also receive needed moisture from pieces of fruit or other vegetation, which makes up the bulk of their diet. ...
The ABA understands the critical role that NSA plays in gathering intelligence information and protecting our national security, and we acknowledge that during the course of these activities, it is inevitable that certain communications between U.S. law firms and their clients may be collected or otherwise obtained by the agency. However, irrespective of the accuracy of the recent press reports, we would like to work with NSA on this issue and urge the agency not to actively seek confidential communications between U.S. law firms and their clients. In addition, if NSA obtains such confidential information inadvertently-or such information is obtained by foreign intelligence services or others and then shared with NSA-we would expect NSA to respect the privilege and take all appropriate steps to ensure that any such privileged information is not further disseminated to other agencies or any other third parties. ...
Cell-permeable. A potent, non-competitive inhibitor of p300 and PCAF (p300/CBP-associated factor) histone acetyltransferase (HAT) activities (IC50~8.5 µM and ~5 µM, res
Source Naturals Coenzyme Q10 100mg Vegetarian Softgels 60 softgel vegi oenzyme Q10 is a crucial component in the cellular energy production cycle. Research indicates that ...
Since last weeks revelations about the NSA, skeptics have questioned whether expansive intelligence powers might really lead to civil liberties abuses ...
According to newly-declassified court orders from the Foreign Intelligence Surveillance Court (FISC), the National Security Agency (NSA) was (and may still be) tipping off the FBI at least two to three times per day going back at least to 2006 ...
Time to let the NSA know for sure I am a balding, middle aged, white male, heavy set, bad attitude, grumpy, middle class and a home grown American Terroris...
with assistance from Rainey Reitman. A year ago, President Obama made tepid promises to reform the NSA (check out our analysis of those commitments). Today, he followed up with more specifics. What do you need to know? His reform plan: Fails to fix the problem of unconstitutional...

Chorioallantoic membrane - WikipediaChorioallantoic membrane - Wikipedia

The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of ... with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases ... Ribatti D (2008). "Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis". Int Rev Cell Mol Biol. 270: ... Chorioallantoic membranes from developing chicken eggs are routinely used in biological and biomedical research to investigate ...
more infohttps://en.wikipedia.org/wiki/Chorioallantoic_membrane

IJMS  | Free Full-Text | Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified...IJMS | Free Full-Text | Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified...

Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM ... Figure 2. (a) Day 3 chick embryo; (b) Ovarian cancer cells and matrigel graft on the chick chorioallantoic membrane (CAM) on ... Figure 2. (a) Day 3 chick embryo; (b) Ovarian cancer cells and matrigel graft on the chick chorioallantoic membrane (CAM) on ... Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian ...
more infohttp://www.mdpi.com/1422-0067/13/8/9959/htm

Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is...Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is...

keywords = "Angiogenesis, Chorioallantoic membrane, Endostatin, Multiple myeloma",. author = "Domenica Mangieri and Beatrice ... T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is ... Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is ... Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is ...
more infohttps://moh-it.pure.elsevier.com/en/publications/angiogenic-activity-of-multiple-myeloma-endothelial-cells-in-vivo

Smallpox (variola, variola major, variola minor, variola vera, alastrim, the pox, variole, viruela, pocken, blatternSmallpox (variola, variola major, variola minor, variola vera, alastrim, the pox, variole, viruela, pocken, blattern

Smallpox virus grown on chick chorioallantoic membrane shows characteristic pocks (DC/Dr David Kirsh). ... Variola inoculated onto chick chorioallantoic membrane produces characteristic "hemorrhagic" pocks (. Figure 9. ). ... There is extensive bleeding into the skin, nasal and oral mucous membranes, and gastrointestinal tract. This form usually ... Early: the prodrome is characterized by petechial hemorrhages in the skin or mucous membranes. Subconjunctival bleeding may ...
more infohttp://www.psychiatryadvisor.com/dermatology/smallpox-variola-variola-major-variola-minor-variola-vera-alastrim-the-pox-variole-viruela-pocken-blattern/article/591502/

Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli...Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli...

... the pro-angiogenic proprieties of recombinant VEGF165 we performed ex vivo angiogenesis using Chick Chorioallantoic Membrane ( ... Overproduction of membrane-integral undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus * RKIP and YY1: The good ...
more infohttp://atlasofscience.org/expression-purification-and-functionality-of-bioactive-recombinant-human-vascular-endothelial-growth-factor-vegf165-in-e-coli/

Angiogenic activity in the chorioallantoic membrane (CA | Open-iAngiogenic activity in the chorioallantoic membrane (CA | Open-i

Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C ... fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI- ... fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI- ... Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4401142_jcmm0012-1023-f1&req=4

Specific antigens from tumors after prolonged cultivation on the chorioallantoic membrane of the chick embryo | SpringerLinkSpecific antigens from tumors after prolonged cultivation on the chorioallantoic membrane of the chick embryo | SpringerLink

... sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the ... Specific antigens from tumors after prolonged cultivation on the chorioallantoic membrane of the chick embryo. ... sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the ...
more infohttps://link.springer.com/article/10.1007/BF00781953

Figure 6 | Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic...Figure 6 | Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic...

Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane ... Effect of ethyl acetate (EtOAc) fraction of V. tricolor on the number and diameter of vessels in chorioallantoic membrane (CAM ... Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane. ... of the ethyl acetate fraction was injected into the chorioallantoic sac. 1, 2, and 3 are representative of heart, artery, and ...
more infohttps://www.hindawi.com/journals/bmri/2014/625792/fig6/

The Chick Embryo Chorioallantoic Membrane In Study Of Angiogenesis And MetastasisThe Chick Embryo Chorioallantoic Membrane In Study Of Angiogenesis And Metastasis

Our site doesnt host any file or offering download for any book. All of the download are provided by our adversiser. For copyright & DMCA report, please send email to ΡRΟJΕCΤ.DΟΜΙΝΟ@HΟΜΑΙL.CΟΜ ...
more infohttp://www.turismoresponsable.net/the/the_chick_embryo_chorioallantoic_membrane_in_study_of_angiogenesis_and_metastasis.pdf

A novel angiogenesis model for screening anti-angiogenic compounds: the chorioallantoic membrane/feather bud assay. | Sigma...A novel angiogenesis model for screening anti-angiogenic compounds: the chorioallantoic membrane/feather bud assay. | Sigma...

A novel angiogenesis model for screening anti-angiogenic compounds: the chorioallantoic membrane/feather bud assay.. [Haiming ... novel and rapid method to evaluate the anti-angiogenic activity of new agents consisting of a combined chorioallantoic membrane ...
more infohttps://www.sigmaaldrich.com/catalog/papers/20514398

Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay |...Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay |...

... isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay - Volume 93 Issue 3 - Serafim Kiriakidis ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay. * ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay ... Novel tempeh (fermented soyabean) isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay ...
more infohttps://www.cambridge.org/core/journals/british-journal-of-nutrition/article/novel-tempeh-fermented-soyabean-isoflavones-inhibit-in-vivo-angiogenesis-in-the-chicken-chorioallantoic-membrane-assay/7EE28C69A2235E7B4EA6FE79D4E2A1EA

Ex Vivo Culture of Lung Buds on the Chorioallantoic Membrane of the Avian Embryo  - CaltechAUTHORSEx Vivo Culture of Lung Buds on the Chorioallantoic Membrane of the Avian Embryo - CaltechAUTHORS

Conclusions: The chorioallantoic membrane culture system can be used to sustain the growth and development of isolated chick ... Methods: We have explanted the lung buds of developing day 4 chick or quail embryos onto the chorioallantoic membranes of host ... Results: We have demonstrated that developing lung buds explanted to chorioallantoic membranes continue to develop further, in ... Ex Vivo Culture of Lung Buds on the Chorioallantoic Membrane of the Avian Embryo ...
more infohttps://authors.library.caltech.edu/73749/

Angiogenesis and Diabetes: Different Responses to Pro-Angiogenic Factors in the Chorioallantoic Membrane Assay - Molecular...Angiogenesis and Diabetes: Different Responses to Pro-Angiogenic Factors in the Chorioallantoic Membrane Assay - Molecular...

Angiogenesis and Diabetes: Different Responses to Pro-Angiogenic Factors in the Chorioallantoic Membrane Assay ...
more infohttp://molmed.org/journal/articles/3/1230

Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal...Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal...

Epub ahead of print] Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein ... Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal ... Fifteen Influenza A virus reference subtypes, H1 to H15, infected chicken embryonated chorioallantoic membranes (CAM) fixed by ... Novel application of Influenza A virus inoculated chorioallantoic membrane to characterize a nucleoprotein specific monoclonal ...
more infohttps://flutrackers.com/forum/forum/welcome-to-the-scientific-library/diagnosis-testing/803047-novel-application-of-influenza-a-virus-inoculated-chorioallantoic-membrane-to-characterize-a-nucleoprotein-specific-monoclonal-antibody-for-immunohistochemistry-assay

A quantitative study of the chorioallantoic membrane reaction in the chick embryo | Proceedings of the Royal Society of London...A quantitative study of the chorioallantoic membrane reaction in the chick embryo | Proceedings of the Royal Society of London...

A quantitative study of the chorioallantoic membrane reaction in the chick embryo. L. W. Coppleson, D. Michie, Sir Peter ... When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing ... A quantitative study of the chorioallantoic membrane reaction in the chick embryo ... A quantitative study of the chorioallantoic membrane reaction in the chick embryo. ...
more infohttp://rspb.royalsocietypublishing.org/content/163/993/555

Angiogenesis within Stem Cell-Seeded Silk Scaffolds Cultured on the Chorioallantoic Membrane and Visualized by 3D Imaging -...Angiogenesis within Stem Cell-Seeded Silk Scaffolds Cultured on the Chorioallantoic Membrane and Visualized by 3D Imaging -...

Angiogenesis within Stem Cell-Seeded Silk Scaffolds Cultured on the Chorioallantoic Membrane and Visualized by 3D Imaging. ... It includes seeding of stem cells into silk fibroin scaffolds, angiogenesis assay on the chorioallantoic membrane (CAM) of ...
more infohttp://www.currentprotocols.com/WileyCDA/CPUnit/refId-sc01f19.html

Capillaries in the Chicken Chorioallantoic Membrane - Endothelial CellsCapillaries in the Chicken Chorioallantoic Membrane - Endothelial Cells

The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane. It serves as a gas exchange surface through the ... Capillaries in the Chicken Chorioallantoic Membrane. Last Updated on Thu, 09 Oct 2014 , Endothelial Cells ... At day 14, the capillary plexus is located at the surface of the ectoderm adjacent to the shell membrane. The blood circulation ... Between days 9 and 13 the arteriolar endothelium displays more extensive junctional complexes with multiple membrane contact ...
more infohttps://www.alpfmedical.info/endothelial-cells/capillaries-in-the-chicken-chorioallantoic-membrane.html

3D chick embryo chorioallantoic membrane model as an in vivo model to study morphological and histopathological features of...3D chick embryo chorioallantoic membrane model as an in vivo model to study morphological and histopathological features of...

The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform ... The chick embryo chorioallantoic membrane (CAM) model is well-known in human medicine, first described by Rous and Murphy [8]. ... The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform ... Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian ...
more infohttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1114-4

Chick embryo chorioallantoic membrane model systems to study a...Chick embryo chorioallantoic membrane model systems to study a...

Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis.: Since their ... Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis.. Authors * Deryugina, ... The ability of the chick embryos chorioallantoic membrane (CAM) to efficiently support the growth of inoculated xenogenic ... Since their introduction almost a century ago, chick embryo model systems involving the technique of chorioallantoic grafting ...
more infohttps://www.mysciencework.com/publication/show/chick-embryo-chorioallantoic-membrane-model-systems-study-visualize-human-tumor-cell-metastasis-98db0e54

VITRIFICATION AND CHORIOALLANTOIC MEMBRANE (CAM) CULTURE OF BOVINE OVARIAN TISSUEVITRIFICATION AND CHORIOALLANTOIC MEMBRANE (CAM) CULTURE OF BOVINE OVARIAN TISSUE

We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than ... or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. Follicles from control and vitrified tissue were ... subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. ...
more infohttps://ecommons.usask.ca/handle/10388/ETD-2015-05-2051

Histoplasma Capsulatum: Its Cultivation on the Chorio-Allantoic Membrane of the Developing Chick and Resultant Lesions1 | The...Histoplasma Capsulatum: Its Cultivation on the Chorio-Allantoic Membrane of the Developing Chick and Resultant Lesions1 | The...

The chorio-allantoic membrane of the developing chick was successfully inoculated with a seven year old culture of Histoplasma ... Histoplasma Capsulatum: Its Cultivation on the Chorio-Allantoic Membrane of the Developing Chick and Resultant Lesions 1 * ... 1. The chorio-allantoic membrane of the developing chick was successfully inoculated with a seven year old culture of ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1941.s1-21.627

Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane<...Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane<...

Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane. Clinical and Experimental ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ... Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic ...
more infohttps://moh-it.pure.elsevier.com/en/publications/mast-cells-and-their-secretory-granules-are-angiogenic-in-the-chi

Blood flow shapes intravascular pillar geometry in the chick chorioallantoic membrane | Vascular Cell | Full TextBlood flow shapes intravascular pillar geometry in the chick chorioallantoic membrane | Vascular Cell | Full Text

... in the chick chorioallantoic membrane. Pillars comprised 0.02 to 0.5% of the vascular system in 2-dimensional projection and ... by blood flow have both a limiting and permissive influence on pillar development in the chick chorioallantoic membrane. ... In the chick chorioallantoic membrane, a common model of microvascular network development, the extra-embryonic area undergoes ... Tufan AC, Satiroglu-Tufan NL: The chick embryo chorioallantoic membrane as a model system for the study of tumor angiogenesis, ...
more infohttps://vascularcell.biomedcentral.com/articles/10.1186/2040-2384-2-11

High-resolution Magnetic Resonance Imaging analysis of breast cancer xenograft on the chick chorioallantoic membraneHigh-resolution Magnetic Resonance Imaging analysis of breast cancer xenograft on the chick chorioallantoic membrane

... Autor. ... High-resolution Magnetic Resonance Imaging analysis of breast cancer xenograft on the chick chorioallantoic membrane. dc.title ... of the chicken embryo and also tumor xenograft on the chorioallantoic membrane (CAM). Tumor growth monitoring was firstly ...
more infohttps://oparu.uni-ulm.de/xmlui/handle/123456789/3764?show=full

Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial...Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial...

Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial ... Also, all donepezil, rivastigmine and galantamine significantly increased the number of vessels in the chorioallantoic membrane ... Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial ... Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial ...
more infohttp://libros.duhnnae.com/2017/jun9/14986816488-Acetylcholinesterase-Inhibitors-Promote-Angiogenesis-in-Chick-Chorioallantoic-Membrane-and-Inhibit-Apoptosis-of-Endothelial-Cells.php
  • The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes , such as birds and reptiles . (wikipedia.org)
  • The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. (usask.ca)
  • Results: Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the chorioallantoic membrane. (elsevier.com)
  • Also, all donepezil, rivastigmine and galantamine significantly increased the number of vessels in the chorioallantoic membrane when injected into fertilized eggs. (duhnnae.com)
  • As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen , to developing embryos . (wikipedia.org)
  • In contrast, MMP-2 lacks a serine protease recognition motif ( 10 ) and is activated at the cell surface of invasive cells by a multimeric receptor/activation complex consisting of the tissue inhibitor of metalloproteinase 2 (TIMP-2), the membrane type 1 MMP (MT1-MMP), and integrin αvβ3 ( 11 ). (pnas.org)
  • Role of FGF-2 in chorioallantoic membrane vascularization 4. (moluna.de)