Chondroitin Lyases
Enzymes which catalyze the elimination of delta-4,5-D-glucuronate residues from polysaccharides containing 1,4-beta-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages thereby bringing about depolymerization. EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. EC 4.2.2.5 acts on chondroitin sulfate A and C.
Chondroitinases and Chondroitin Lyases
Bacteroides
Chondroitin Sulfates
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Chondroitin
Chondroitin Sulfate Proteoglycans
Lyases
Chondroitin ABC Lyase
Glycosaminoglycans
Dermatan Sulfate
Action of chondroitinases. II. Numerical calculation of the degree of multiple attack. (1/149)
Further investigation was carried out on the action patterns of two chondroitinase-AC [EC 4.2.2.5.] preparations obtained from Arthrobacter aurescens and Flavobacterium heparinum. To infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by Robyt and French ((1967) Arch. Biochem. Biophys. 122, 8-16). It was shown that the degree of multiple attack (DM) is represented by the ratio of the initial velocity of number-average degree of scission to that of viscosity-average degree of scission. By this method, DM for A-Chase was estimated to be 3.03 and for F-chase, 1.31. (+info)Substrate specificity studies of Flavobacterium chondroitinase C and heparitinases towards the glycosaminoglycan--protein linkage region. Use of a sensitive analytical method developed by chromophore-labeling of linkage glycoserines using dimethylaminoazobenzenesulfonyl chloride. (2/149)
Bacterial chondroitinases and heparitinases are potentially useful tools for structural studies of chondroitin sulfate and heparin/heparan sulfate. Substrate specificities of Flavobacterium chondroitinase C, as well as heparitinases I and II, towards the glycosaminoglycan-protein linkage region -HexA-HexNAc-GlcA-Gal-Gal-Xyl-Ser (where HexA represents glucuronic acid or iduronic acid and HexNAc represents N-acetylgalactosamine or N-acetylglucosamine) were investigated using various structurally defined oligosaccharides or oligosaccharide-serines derived from the linkage region. In the case of oligosaccharide-serines, they were labeled with a chromophore dimethylaminoazobenzenesulfonyl chloride (DABS-Cl), which stably reacted with the amino group of the serine residue and rendered high absorbance for microanalysis. Chondroitinase C cleaved the GalNAc bond of the pentasaccharides or hexasaccharides derived from the linkage region of chondroitin sulfate chains and tolerated sulfation of the C-4 or C-6 of the GalNAc residue and C-6 of the Gal residues, as well as 2-O-phosphorylation of the Xyl residue. In contrast, it did not act on the GalNAc-GlcA linkage when attached to a 4-O-sulfated Gal residue. Heparitinase I cleaved the innermost glucosaminidic bond of the linkage region oligosaccharide-serines of heparin/heparan sulfate irrespective of substitution by uronic acid, whereas heparitinase II acted only on the glucosaminidic linkages of the repeating disaccharide region, but not on the innermost glucosaminidic linkage. These defined specificities of chondroitinase C, as well as heparitinases I and II, will be useful for preparation and structural analysis of the linkage oligosaccharides. (+info)Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. (3/149)
Radioactivity was significantly incorporated from ascorbate 2-[35S]sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. The extent of incorporation was comparable with that from inorganic [35S]sulfate. The radioactive chondroitin sulfate formed from ascorbate 2-[35S]sulfate gave two radioactive disaccharides on chondroitinase-ABC [EC 4.2.2.4] digestion. The incorporation was markedly decreased by inorganic sulfate. The time course of incorporation from ascorbate 2-[35S]sulfate and inorganic [35S]sulfate into chondroitin sulfate and the constituent disaccharides suggest that the incorporation rates from the two radioactive substances are different. (+info)Sulphation heterogeneity in the trisaccharide (GalNAcSbeta1, 4GlcAbeta1,3GalNAcS) isolated from the non-reducing terminal of human aggrecan chondroitin sulphate. (4/149)
We report here the isolation and sulphation isomer analyses of trisaccharides GalNAcS(beta1,4)GlcA(beta1,3)GalNAcS (in which S indicates sulphate) derived from the non-reducing termini of aggrecan chondroitin sulphate. Rat chondrosarcoma and human aggrecans were digested for 1 h at 37 degrees C with 30 micro-units of endo-chondroitinase ABC per microgram of chondroitin sulphate, and trisaccharides were isolated from the digests by ToyoPearl HW40S gel-filtration chromatography. Four trisaccharide species were identified; their sulphation isomer compositions, as determined by digestion with chondroitinase ACII and fluorescence-based ion-exchange HPLC, were GalNAc4Sbeta1,4GlcAbeta1,3GalNAc4S, GalNAc4Sbeta1,4GlcAbeta1,3GalNAc6S, GalNAc4,6Sbeta1,4GlcAbeta1, 3GalNAc4S and GalNAc4,6Sbeta1,4GlcAbeta1,3GalNAc6S. The abundances of such sequences in chondroitin sulphate on aggrecan from normal (foetal to 72 years of age) and from osteoarthritic human knee cartilages were also established. The results showed that non-reducing terminal GalNAc4S or GalNAc4,6S can be linked to either a 4-sulphated or a 6-sulphated disaccharide, suggesting that the sulphation of the last disaccharide might not have a direct effect on the specificity of chondroitin sulphate terminal GalNAc sulphotransferases. Furthermore, for each aggrecan preparation examined, the 4S-to-6S ratio of all chain interior disaccharides was equivalent to that in the last repeating disaccharides at the non-reducing terminus, suggesting that neither chondroitin 4-sulphotransferase nor chondroitin 6-sulphotransferase shows preferential activity near the chain terminus. (+info)Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum. (5/149)
In medium supplemented with chondroitin sulfate, Flavobacterium heparinum synthesizes and exports two chondroitinases, chondroitinase AC (chondroitin AC lyase; EC 4.2.2.5) and chondroitinase B (chondroitin B lyase; no EC number), into its periplasmic space. Chondroitinase AC preferentially depolymerizes chondroitin sulfates A and C, whereas chondroitinase B degrades only dermatan sulfate (chondroitin sulfate B). The genes coding for both enzymes were isolated from F. heparinum and designated cslA (chondroitinase AC) and cslB (chondroitinase B). They were found to be separated by 5.5 kb on the chromosome of F. heparinum, transcribed in the same orientation, but not linked to any of the heparinase genes. In addition, the synthesis of both enzymes appeared to be coregulated. The cslA and cslB DNA sequences revealed open reading frames of 2,103 and 1,521 bp coding for peptides of 700 and 506 amino acid residues, respectively. Chondroitinase AC has a signal sequence of 22 residues, while chondroitinase B is composed of 25 residues. The mature forms of chondroitinases AC and B are comprised of 678 and 481 amino acid residues and have calculated molecular masses of 77,169 and 53,563 Da, respectively. Truncated cslA and cslB genes have been used to produce active, mature chondroitinases in the cytoplasm of Escherichia coli. Partially purified recombinant chondroitinases AC and B exhibit specific activities similar to those of chondroitinases AC and B from F. heparinum. (+info)A fingerprinting method for chondroitin/dermatan sulfate and hyaluronan oligosaccharides. (6/149)
A previously published method for the analysis of glycosaminoglycan disaccharides by high pH anion exchange chromatography (Midura,R.J., Salustri,A., Calabro,A., Yanagishita,M. and Hascall,V.C. (1994), Glycobiology,4, 333-342) has been modified and calibrated for chondroitin and dermatan sulfate oligosaccharides up to hexasaccharide in size and hyaluronan oligosaccharides up to hexadecasaccharide. For hyaluronan oligosaccharides chain length controls elution position; however, for chondroitin and dermatan sulfate oligosaccharides elution times primarily depend upon the level of sulfation, although chain length and hence charge density plays a role. The sulfation position of GalNAc residues within an oligosaccharide is also important in determining its elution position. Compared to 4-sulfation a reducing terminal 6-sulfate retards elution; however, when present on an internal GalNAc residue it is the 4-sulfate containing oligosaccharide which elutes later. These effects allow discrimination between oligosaccharides differing only in the position of GalNAc sulfation. Using this simple methodology, a Dionex CarboPac PA-1 column with NaOH/NaCl eluents and detection by absorbance at 232 nm, a quantitative analytical fingerprint of a chondroitin/dermatan sulfate chain may be obtained, allowing a determination of the abundance of chondroitin sulfate, dermatan sulfate, and hyaluronan along with an analysis of structural features with a linear response to approximately 0.1 nmol. The method may readily be calibrated using either commercial disaccharides or the di- and tetrasaccharide products of a limit digest of commercial chondroitin sulfate by chondroitin ABC endolyase. Commercially available and freshly prepared shark, whale, bovine, and human cartilage chondroitin sulfates have been examined by this methodology and we have confirmed that freshly isolated shark cartilage CS contains significant amounts of the biologically important GlcA2Sbeta(1-3)GalNAc6S structure. (+info)Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection. (7/149)
Respiratory syncytial virus (RSV) is an important human respiratory pathogen, particularly in infants. Glycosaminoglycans (GAGs) have been implicated in the initiation of RSV infection of cultured cells, but it is not clear what type of GAGs and GAG components are involved, whether the important GAGs are on the virus or the cell, or what the magnitude is of their contribution to infection. We constructed and rescued a recombinant green fluorescent protein (GFP)-expressing RSV (rgRSV) and used this virus to develop a sensitive system to assess and quantify infection by flow cytometry. Evaluation of a panel of mutant Chinese hamster ovary cell lines that are genetically deficient in various aspects of GAG synthesis showed that infection was reduced up to 80% depending on the type of GAG deficiency. Enzymatic removal of heparan sulfate and/or chondroitin sulfate from the surface of HEp-2 cells also reduced infection, and the removal of both reduced infection even further. Blocking experiments in which RSV was preincubated with various soluble GAGs revealed the relative blocking order of: heparin > heparan sulfate > chondroitin sulfate B. Iduronic acid is a component common to these GAGs. GAGs that do not contain iduronic acid, namely, chondroitin sulfate A and C and hyaluronic acid, did not inhibit infection. A role for iduronic acid-containing GAGs in RSV infection was confirmed by the ability of basic fibroblast growth factor to block infection, because basic fibroblast growth factor binds to GAGs containing iduronic acid. Pretreatment of cells with protamine sulfate, which binds and blocks GAGs, also reduced infection. In these examples, infection was reduced by pretreatment of the virus with soluble GAGs, pretreatment of the cells with GAG-binding molecules, pretreatment of the cells with GAG-destroying enzymes or in cells genetically deficient in GAGs. These results establish that the GAGs involved in RSV infection are present on the cell rather than on the virus particle. Thus, the presence of cell surface GAGs containing iduronic acid, like heparan sulfate and chondroitin sulfate B, is required for efficient RSV infection in cell culture. (+info)Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates. (8/149)
Sulfated glycosaminoglycans were isolated from 23 species of 13 phyla of invertebrates and characterized by their electrophoretic migration in three different buffer systems coupled with enzymatic degradation using bacterial heparinase, heparitinases and chondroitinase AC. Heparan sulfate is a ubiquitous compound present in all species analyzed whereas chondroitin sulfate was present in 20 species and heparin-like compounds in 12 species of the invertebrates. The heparin-like compounds were purified from the echinoderm Mellita quinquisperforata (sand dollar) and the crustacean Ucides cordatus (crab) with anticoagulant activities of 60 and 52 IU/mg, respectively. Degradation of these heparins with heparinase produced significant amounts of the trisulfated disaccharide typical of mammalian heparins. This was confirmed by 13C-NMR spectroscopy of the crab heparin. An updated phylogenetic tree of the distribution of sulfated glycosaminoglycans in the animal kingdom is also presented. (+info)
Chondroitin ABC lyase
... (EC 4.2.2.20 EC 4.2.2.21, chondroitinase, chondroitin ABC eliminase, chondroitinase ABC) is an enzyme ... Chondroitin+ABC+lyase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 4.2.2, ... with systematic name chondroitin ABC lyase. This enzyme catalyses the following chemical reaction Eliminative degradation of ... chondroitin 6-sulfate and dermatan sulfate. Following a spinal cord injury, this enzyme can be used to erode scar tissue that ...
Chondroitin sulfate ABC lyase
... may refer to: Chondroitin ABC lyase, an enzyme Chondroitin-sulfate-ABC exolyase, an enzyme ... an enzyme This disambiguation page lists articles associated with the title Chondroitin sulfate ABC lyase. If an internal link ...
Chondroitin B lyase
The enzyme chondroitin B lyase (EC 4.2.2.19) catalyzes the following process: Eliminative cleavage of dermatan sulfate ... The systematic name of this enzyme class is chondroitin B lyase. Other names in common use include chondroitinase B, ChonB, and ... V, Sasisekharan R, Cygler M (2004). "The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides ... characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum". Biochem. J. 312 (Pt 2 ...
Chondroitin AC lyase
... chondroitin sulfate lyase, chondroitin AC eliminase, chondroitin AC lyase, chondroitinase AC, and ChnAC. As of late 2007, 11 ... The enzyme chondroitin AC lyase (EC 4.2.2.5) catalyzes the chemical reaction Eliminative degradation of polysaccharides ... The systematic name of this enzyme class is chondroitin AC lyase. Other names in common use include chondroitinase (ambiguous ... "Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced ...
Chondroitin-sulfate-ABC exolyase
... chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ... This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic ... "Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially ... ABC exoeliminase, chondroitin sulfate ABC exolyase, and ChS ABC lyase II. Yamagata T, Saito H, Habuchi O, Suzuki S (1968). " ...
Chondroitin-sulfate-ABC endolyase
... chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ... specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin- ... "Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially ... ABC endoeliminase, chondroitin sulfate ABC endolyase, and ChS ABC lyase I. Yamagata T, Saito H, Habuchi O, Suzuki S (1968). " ...
List of MeSH codes (D08)
... chondroitin lyases MeSH D08.811.520.241.700.350.500.500 - chondroitin abc lyase MeSH D08.811.520.241.700.512 - heparin lyase ... polysaccharide-lyases MeSH D08.811.520.241.700.350 - chondroitinases and chondroitin lyases MeSH D08.811.520.241.700.350.500 - ... tyrosine phenol-lyase MeSH D08.811.520.232.300 - amidine-lyases MeSH D08.811.520.232.300.200 - adenylosuccinate lyase MeSH ... ammonia-lyases MeSH D08.811.520.232.400.200 - aspartate ammonia-lyase MeSH D08.811.520.232.400.350 - ethanolamine ammonia-lyase ...
Chondroitinase
... an enzyme Chondroitin ABC lyase, an enzyme This set index page lists enzyme articles associated with the same name. If an ...
CHNB
Ontario Chondroitin B lyase, an enzyme This disambiguation page lists articles associated with the title CHNB. If an internal ...
List of EC numbers (EC 4)
... chondroitin AC lyase EC 4.2.2.6: oligogalacturonide lyase EC 4.2.2.7: heparin lyase EC 4.2.2.8: heparin-sulfate lyase EC 4.2. ... chondroitin ABC lyase) and EC 4.2.2.5 (chondroitin AC lyase) EC 4.2.99.7: Now EC 4.2.3.2, ethanolamine-phosphate phospho-lyase ... chondroitin B lyase EC 4.2.2.20: chondroitin-sulfate-ABC endolyase EC 4.2.2.21: chondroitin-sulfate-ABC exolyase EC 4.2.2.22: ... pectin lyase EC 4.2.2.11: guluronate-specific alginate lyase EC 4.2.2.12: xanthan lyase EC 4.2.2.13: exo-(1→4)-α-D-glucan lyase ...
Heparin
The lyases have mainly been used in heparin/HS studies. The bacterium produces three lyases, heparinases I (EC 4.2.2.7), II (no ... The most prevalent impurity in heparin is dermatan sulfate (DS), also known as chondroitin sulfate B. The building-block of DS ... The lyases cleave heparin/HS by a beta elimination mechanism. This action generates an unsaturated double bond between C4 and ... Danaparoid, a mixture of heparan sulfate, dermatan sulfate, and chondroitin sulfate can be used as an anticoagulant in patients ...
List of EC numbers (EC 3)
EC 3.1.27.6: Now EC 4.6.1.21, Enterobacter ribonuclease}}, since the primary reaction is that of a lyase EC 3.1.27.7: ... unsaturated chondroitin disaccharide hydrolase EC 3.2.1.181: galactan endo-β-1,3-galactanase EC 3.2.1.182: 4-hydroxy-7-methoxy- ... EC 3.1.25.1: deoxyribonuclease (pyrimidine dimer) EC 3.1.25.2: Now EC 4.2.99.18, DNA-(apurinic or apyrimidinic site) lyase EC ... citrate lyase deacetylase EC 3.1.2.17: (S)-methylmalonyl-CoA hydrolase EC 3.1.2.18: ADP-dependent short-chain-acyl-CoA ...
Cathepsin S
It also cleaves chondroitin sulfate, heparan sulfate and proteoglycans of the basal membrane. Cathepsin S plays an active role ... January 2019). "Cystathionine γ Lyase Sulfhydrates the RNA Binding Protein Human Antigen R to Preserve Endothelial Cell ... September 2013). "Binding of chondroitin 4-sulfate to cathepsin S regulates its enzymatic activity". Biochemistry. 52 (37): ...
List of EC numbers (EC 2)
... lyase) S-acetyltransferase EC 2.3.1.50: serine C-palmitoyltransferase EC 2.3.1.51: 1-acylglycerol-3-phosphate O-acyltransferase ... chondroitin 4-sulfotransferase EC 2.8.2.6: choline sulfotransferase EC 2.8.2.7: UDP-N-acetylgalactosamine-4-sulfate ... citrate lyase holo-[acyl-carrier protein] synthase EC 2.7.7.62: adenosylcobinamide-phosphate guanylyltransferase EC 2.7.7.63: ... chondroitin 6-sulfotransferase EC 2.8.2.18: cortisol sulfotransferase EC 2.8.2.19: triglucosylalkylacylglycerol ...
1rwa.1 | SWISS-MODEL Template Library
Crystal structure of Arthrobacter aurescens chondroitin AC lyase ... chondroitin AC lyase: A. SMTL:PDB. SMTL Chain Id:. PDB Chain Id ... Crystal structure of Arthrobacter aurescens chondroitin AC lyase Coordinates. PDB Format Method. X-RAY DIFFRACTION 1.30 Å. ... Lunin, V.V. et al., High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate ...
The role of glucosamine sulfate and chondroitin sulfates in the treatment of degenerative joint disease - PubMed
Chondroitin Lyase from a Marine Arthrobacter sp. MAT3885 for the Production of Chondroitin Sulfate Disaccharides. Kale V, ... The role of glucosamine sulfate and chondroitin sulfates in the treatment of degenerative joint disease G S Kelly. Altern Med ... Effects of chondroitin sulfate and glucosamine. Baccarin RY, Machado TS, Lopes-Moraes AP, Vieira FA, Michelacci YM. Baccarin RY ... Glucosamine and chondroitin sulfate as a possible treatment for osteoarthritis]. Bijlsma JW. Bijlsma JW. Ned Tijdschr Geneeskd ...
MeSH Browser
Chondroitin AC Lyase Chondroitin B Lyase Chondroitin Eliminase Chondroitin Sulfate Lyase Chondroitinase-AC II Registry Number. ... Chondroitin B Lyase Narrower Concept UI. M0004311. Registry Number. 0. Terms. Chondroitin B Lyase Preferred Term Term UI ... Chondroitin Sulfate Lyase Narrower Concept UI. M0004314. Registry Number. 0. Terms. Chondroitin Sulfate Lyase Preferred Term ... Chondroitinases and Chondroitin Lyases [D08.811.520.241.700.350] * Chondroitin Lyases [D08.811.520.241.700.350.500] * ...
Biomarkers Search
DeCS
Lyase, Chondroitin AC. Lyase, Chondroitin B. Lyase, Chondroitin Sulfate. Lyases, Chondroitin. Sulfate Lyase, Chondroitin. ... Chondroitin Sulfate Lyase Entry term(s). Chondroitin AC Lyase Lyase, Chondroitin AC Lyase, Chondroitin Sulfate Sulfate Lyase, ... Chondroitine lyases Entry term(s):. Chondroitin AC Lyase. Chondroitin B Lyase. Chondroitin Eliminase. Chondroitin Sulfate Lyase ... Chondroitin (1970-1975). Lyases (1970-1974). Polysaccharide-Lyases (1975). Public MeSH Note:. 91; was see under CHONDROITINASES ...
MeSH Browser
Chondroitin AC Lyase Chondroitin B Lyase Chondroitin Eliminase Chondroitin Sulfate Lyase Chondroitinase-AC II Registry Number. ... Chondroitin B Lyase Narrower Concept UI. M0004311. Registry Number. 0. Terms. Chondroitin B Lyase Preferred Term Term UI ... Chondroitin Sulfate Lyase Narrower Concept UI. M0004314. Registry Number. 0. Terms. Chondroitin Sulfate Lyase Preferred Term ... Chondroitinases and Chondroitin Lyases [D08.811.520.241.700.350] * Chondroitin Lyases [D08.811.520.241.700.350.500] * ...
Princeton Neuroscience Institute - Research output
- Princeton University
Dorkenwald, S., McKellar, C. E., Macrina, T., Kemnitz, N., Lee, K., Lu, R., Wu, J., Popovych, S., Mitchell, E., Nehoran, B., Jia, Z., Bae, J. A., Mu, S., Ih, D., Castro, M., Ogedengbe, O., Halageri, A., Kuehner, K., Sterling, A. R., Ashwood, Z., & 23 othersZung, J., Brittain, D., Collman, F., Schneider-Mizell, C., Jordan, C., Silversmith, W., Baker, C., Deutsch, D., Encarnacion-Rivera, L., Kumar, S., Burke, A., Bland, D., Gager, J., Hebditch, J., Koolman, S., Moore, M., Morejohn, S., Silverman, B., Willie, K., Willie, R., Yu, S. C., Murthy, M. & Seung, H. S., Jan 2022, In: Nature Methods. 19, 1, p. 119-128 10 p.. Research output: Contribution to journal › Article › peer-review ...
Bio2Vec
Glioma; Glial Cell Tumors
Búsqueda | BVS Bolivia
Chondroitin AC lyase can hydrolyze chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), and hyaluronic acid (HA), making ... As an important glycosaminoglycan hydrolase, chondroitin lyases can hydrolyze chondroitin sulfate (CS) and release ... The structures and applications of microbial chondroitin AC lyase. Fan, Xiao-Man; Zhou, Li-Jian; Huang, Jia-Ying; Zhang, Ye- ... They are further divided into chondroitin AC, ABC, and B lyases according to their spatial structure and substrate specificity ...
NDF-RT Code NDF-RT Name
Sulfatase N0000005998 Chondroitin N0000168038 Chondroitin ABC Lyase N0000168037 Chondroitin Lyases N0000169197 Chondroitin ... Sulfate Proteoglycans N0000010542 Chondroitin Sulfates N0000168036 Chondroitinases and Chondroitin Lyases N0000167602 ... as Amide-N-Donor N0000168008 Carbon-Nitrogen Lyases N0000167775 Carbon-Oxygen Ligases N0000008169 Carbon-Oxygen Lyases ... Lyase N0000168230 DNA-Activated Protein Kinase N0000169224 DNA-Binding Proteins N0000180242 DNA-Cytosine Methylases N0000168286 ...
ABORTIFACIENT AGENTS ABORTIFACIENT AGENTS
HYDROLASES CHONDROITINASES AND CHONDROITIN LYASES HYDROLASES CHONDROITINSULFATASES HYDROLASES CHYMOPAPAIN HYDROLASES CHYMOSIN ... ANTINEOPLASTIC AND IMMUNOSUPPRESSIVE AG PHENYLALANINE AMMONIA-LYASE ANTINEOPLASTIC AND IMMUNOSUPPRESSIVE AG PHLEOMYCINS ... ANTINEOPLASTIC AGENTS PHENYLALANINE AMMONIA-LYASE ANTINEOPLASTIC AGENTS PHLEOMYCINS ANTINEOPLASTIC AGENTS PHOSPHORAMIDE ...
Arylsulfatases | Profiles RNS
Lexical Tools
Lyase, Chondroitin ABC Lyase, Chondroitin AC,Lyase, Chondroitin AC Lyase, Chondroitin B,Lyase, Chondroitin B Lyase, Chondroitin ... Chondroitin ABC eliminase Chondroitin ABC lyase,Chondroitin ABC lyase Chondroitin AC Lyase,Chondroitin AC Lyase Chondroitin AC ... Chondroitin AC eliminase Chondroitin AC lyase,Chondroitin AC lyase Chondroitin B Lyase,Chondroitin B Lyase Chondroitin ... cathepsin B chondroitin ABC lyase,chondroitin ABC lyase chondroitin AC lyase,chondroitin AC lyase chondroitin sulfate, ...
TERM
Chondroitin Chondroitin ABC Lyase Chondroitin Lyases Chondroitin Sulfate Proteoglycans Chondroitin Sulfates Chondroitinases and ... Carbon-Nitrogen Lyases Carbon-Oxygen Ligases Carbon-Oxygen Lyases Carbon-Sulfur Ligases Carbon-Sulfur Lyases Carbonated ... Chondroitin Lyases Chondroitinsulfatases Chondroma Chondromalacia Patellae Chondromatosis Chondromatosis, Synovial ... Lyase DNA-Activated Protein Kinase DNA-Binding Proteins DNA-Cytosine Methylases DNA-Directed DNA Polymerase DNA-Directed RNA ...
Thick Acsending Limb Transcriptomic Database
Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes ( ... "hydroxymethylglutaryl-CoA lyase, mitochondrial precursor" Hmgcl Rattus norvegicus Involved in the catabolism of branched amino ... 112284 1.34 chondroitin sulfate proteoglycan 4 precursor Cspg4 Rattus norvegicus " Proteoglycan playing a role in cell ... Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post- ...
Selected Publications - Structure Function Group
Xu, D., Song, D., PEDERSEN, L.C. & Liu, J. Mutational study of heparan sulfate 2-O-sulfotransferase and chondroitin sulfate 2-O ... Structural insight into the DNA polymerase β deoxyribose phosphate lyase mechanism. DNA Repair, 4:1347-1357 (2005). [Abstract ... Mutational study of heparan sulfate 2-O-sulfotransferase and chondroitin sulfate 2-O-sulfotransferase. J Biol Chem, 282:8356- ... Structural insight into the DNA polymerase β deoxyribose phosphate lyase mechanism. DNA Repair, 4:1347-1357 (2005).] ...
Pharos : Target List
Lyase. 2 Select filter option. Adenine DNA glycosylase. 1 Select filter option. ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase ... Chondroitin sulfate/dermatan sulfate metabolism. 5 Select filter option. CS/DS degradation. 5 Select filter option. DNA Repair ... chondroitin sulfate catabolic process. 5 Select filter option. glycosaminoglycan catabolic process. 5 Select filter option. ...
Pharos : Target List
Lyase. 2 Select filter option. Adenine DNA glycosylase. 1 Select filter option. ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase ... Chondroitin sulfate/dermatan sulfate metabolism. 5 Select filter option. CS/DS degradation. 5 Select filter option. DNA Repair ... chondroitin sulfate catabolic process. 5 Select filter option. glycosaminoglycan catabolic process. 5 Select filter option. ...
Lexical Tools
Lyase, Chondroitin ABC Lyase, Chondroitin AC,Lyase, Chondroitin AC Lyase, Chondroitin B,Lyase, Chondroitin B Lyase, Chondroitin ... Chondroitin ABC eliminase Chondroitin ABC lyase,Chondroitin ABC lyase Chondroitin AC Lyase,Chondroitin AC Lyase Chondroitin AC ... Chondroitin AC eliminase Chondroitin AC lyase,Chondroitin AC lyase Chondroitin B Lyase,Chondroitin B Lyase Chondroitin ... cathepsin B chondroitin ABC lyase,chondroitin ABC lyase chondroitin AC lyase,chondroitin AC lyase chondroitin sulfate, ...
Anti glomerular basement membrane disease. Medical search
Polysaccharide-Lyases. A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in ... Chondroitin Sulfate Proteoglycans. Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing ... Focal SegmentalProteoglycansCell MembraneImmunoglobulin GLupus NephritisChondroitin Sulfate ProteoglycansKidney TubulesKidney ... AntinuclearGlycoproteinsComplement System ProteinsCarbohydratesPolysaccharide-LyasesRNA, Messenger ...
VITAMIN C AND CANCER: THE NAKED MOLE-RAT CONNECTION | Earth Medicine : Earth Medicine
2003) Effects of ascorbic acid and analogs on the activity of testicular hyaluronidase and hyaluronan lyase on hyaluronan, J ... this molecule from its wide use in reversing arthritis along with other glycosaminoglycans such as glucosamine and chondroitin ... Novel 6-O-acylated vitamin C derivatives as hyaluronidase inhibitors with selectivity for bacterial lyases, Bioorg Med Chem ...
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How do glucosamine, chondroitin sulfate, and MSM work to reduce inflammation and rebuild cartilage tissue? Glucosamine, ... This supplement has been shown to block the enzyme Citrate Lyase, which is responsible for converting unused carbohydrates into ... Chondroitin sulfate is a complex carbohydrate that is also found naturally in the body. It helps to keep cartilage healthy by ... Another promising supplement is chondroitin and msm, which helps to reduce inflammation and ease the symptoms of joint pain. ...
Human GSTa4(Glutathione S Transferase Alpha 4) ELISA Kit - Galician environmental technology platform (invitation)
Human MCSP(Melanoma Associated Chondroitin Sulfate Proteoglycan) ELISA Kit. *Human MFGE8(Milk Fat Globule EGF Factor 8) ELISA ... Human CSE(Cystathionine Gamma Lyase) ELISA Kit. *Human CTSC(Cathepsin C) ELISA Kit ... Human MCSP(Melanoma Associated Chondroitin Sulfate Proteoglycan) ELISA Kit. *Human MFGE8(Milk Fat Globule EGF Factor 8) ELISA ...
Dermatan sulfate7
- 28. Chondroitin sulfate/dermatan sulfate sulfatases from mammals and bacteria. (nih.gov)
- 33. Interaction of chondroitin sulfate and dermatan sulfate from various biological sources with heparin-binding growth factors and cytokines. (nih.gov)
- 35. Combining size-exclusion chromatography and fully automated chip-based nanoelectrospray quadrupole time-of-flight tandem mass spectrometry for structural analysis of chondroitin/dermatan sulfate in human decorin. (nih.gov)
- 38. Degradation of chondroitin sulfate and dermatan sulfate with chondroitin lyases. (nih.gov)
- EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. (nih.gov)
- Chondroitin sulfate (CS)and dermatan sulfate (DS) are negatively charged polysaccharides found abundantly in animal tissue and have been extensively described to play key roles in health and disease. (bvsalud.org)
- In this study, we developed an on-line reverse-phase high-performance liquid chromatography-electrospray ionization-mass spectrometry (RP-HPLC-ESI-MS) separation and structural characterization of hyaluronan (HA)/chondroitin sulfate (CS)/dermatan sulfate (DS) disaccharides released by enzymatic treatment and derivatized with 2-aminoacridone (AMAC), providing a high-resolution system also applicable by using a further fluorimetric detector (Fp) before ESI-MS spectral acquisition. (unboundmedicine.com)
Glucosamine and chondroitin2
- A review of evidence-based medicine for glucosamine and chondroitin sulfate use in knee osteoarthritis. (nih.gov)
- Supplement users may recognise this molecule from its wide use in reversing arthritis along with other glycosaminoglycans such as glucosamine and chondroitin. (earthmedicine.com)
Sulfates4
- Chondroitin sulfates, whether they are absorbed intact or broken into their constituent components, similarly provide additional substrates for the formation of a healthy joint matrix. (nih.gov)
- Evidence also supports the oral administration of chondroitin sulfates for joint disease, both as an agent to slowly reduce symptoms and to reduce the need for non-steroidal anti-inflammatory drugs. (nih.gov)
- The combined use of glucosamine sulfate and chondroitin sulfates in the treatment of degenerative joint disease has become an extremely popular supplementation protocol in arthritic conditions of the joints. (nih.gov)
- Although glucosamine sulfate and chondroitin sulfates are often administered together, there is no information available to demonstrate the combination produces better results than glucosamine sulfate alone. (nih.gov)
Hyaluronate2
- The sequence of chondroitinase AC shows low level of homology to several hyaluronate lyases, which likely share its fold. (rcsb.org)
- The shape of the molecule, distribution of electrostatic potential, the pattern of conservation of the amino acids and the results of mutagenesis of hyaluronate lyases, indicate that the enzymatic activity resides primarily within the N-terminal domain. (rcsb.org)
Proteoglycans1
- Chondroitin sulfate proteoglycans (CSPGs) present a formidable barrier to regrowing axons following spinal cord injury. (bvsalud.org)
Bacteria1
- Bacteria express a large number of GAG-degrading lyases that break the hexosamine-uronic acid bond to create an unsaturated sugar ring. (rcsb.org)
Crystal structure1
- High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism. (expasy.org)
Human3
- Ex vivo desequestration of Plasmodium falciparum-infected erythrocytes from human placenta by chondroitin sulfate A. (nih.gov)
- Chondroitin sulfate proteoglycan expression and binding of Plasmodium falciparum-infected erythrocytes in the human placenta during pregnancy. (nih.gov)
- For this purpose, freshly-prepared human neutrophils and monocytes were treated with heparinase III or chondroitinase ABC to digest heparan sulfate -chains or chondroitin sulfate-chains, respectively, from the leukocyte surfaces. (bvsalud.org)
Active1
- Chondroitin AC lyase (chondroitinase AC, 75 kDa) is highly active toward chondroitin 4-sulfate and chondroitin-6 sulfate. (rcsb.org)
Sulfate10
- These IRBCs bound to purified chondroitin sulfate A (CSA) but not to other extracellular matrix proteins or to other known IRBC receptors. (nih.gov)
- Ex vivo desequestration of Plasmodium falciparum-infected erythrocytes from human placenta by chondroitin sulfate A. (nih.gov)
- Binding affinity of Plasmodium falciparum-infected erythrocytes from infected placentas and laboratory selected strains to chondroitin 4-sulfate. (nih.gov)
- The combined use of glucosamine sulfate and chondroitin sulfates in the treatment of degenerative joint disease has become an extremely popular supplementation protocol in arthritic conditions of the joints. (nih.gov)
- Although glucosamine sulfate and chondroitin sulfates are often administered together, there is no information available to demonstrate the combination produces better results than glucosamine sulfate alone. (nih.gov)
- A review of evidence-based medicine for glucosamine and chondroitin sulfate use in knee osteoarthritis. (nih.gov)
- EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. (bvsalud.org)
- EC 4.2.2.5 acts on chondroitin sulfate A and C. (bvsalud.org)
- As an important glycosaminoglycan hydrolase, chondroitin lyases can hydrolyze chondroitin sulfate (CS) and release disaccharides and oligosaccharides. (bvsalud.org)
- Chondroitin AC lyase can hydrolyze chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), and hyaluronic acid (HA), making it an essential biocatalyst for the preparation of low molecular weight chondroitin sulfate, analysis of the structure of the chondroitin sulfate, treatment of spinal cord injury, and purification of heparin. (bvsalud.org)
Administration1
- Evidence also supports the oral administration of chondroitin sulfates for joint disease, both as an agent to slowly reduce symptoms and to reduce the need for non-steroidal anti-inflammatory drugs. (nih.gov)