Chondroitin Lyases
Enzymes which catalyze the elimination of delta-4,5-D-glucuronate residues from polysaccharides containing 1,4-beta-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages thereby bringing about depolymerization. EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. EC 4.2.2.5 acts on chondroitin sulfate A and C.
Chondroitinases and Chondroitin Lyases
Bacteroides
Chondroitin Sulfates
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Chondroitin
Chondroitin Sulfate Proteoglycans
Lyases
Chondroitin ABC Lyase
Glycosaminoglycans
Dermatan Sulfate
Bacteroides fragilis
Proteus vulgaris
Flavobacteriaceae
Tenacibaculum
Fish Diseases
Virulence
Arteriosclerosis
Lipoproteins
Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.
Atherosclerosis
Lipoproteins, LDL
A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.
Cholesterol
Oligosaccharides
Jehovah's Witnesses
Members of a religious denomination founded in the United States during the late 19th century in which active evangelism is practiced, the imminent approach of the millennium is preached, and war and organized government authority in matters of conscience are strongly opposed (from American Heritage Dictionary of the English Language, 4th ed). Jehovah's Witnesses generally refuse blood transfusions and other blood-based treatments based on religious belief.
Cardiopulmonary Bypass
Protamines
A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)
Thrombelastography
Christianity
The religion stemming from the life, teachings, and death of Jesus Christ: the religion that believes in God as the Father Almighty who works redemptively through the Holy Spirit for men's salvation and that affirms Jesus Christ as Lord and Savior who proclaimed to man the gospel of salvation. (From Webster, 3d ed)
Polysaccharide-Lyases
Fatigue
Crystallography, X-Ray
Pectins
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Databases, Protein
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Decorin
A small leucine-rich proteoglycan that interacts with FIBRILLAR COLLAGENS and modifies the EXTRACELLULAR MATRIX structure of CONNECTIVE TISSUE. Decorin has also been shown to play additional roles in the regulation of cellular responses to GROWTH FACTORS. The protein contains a single glycosaminoglycan chain and is similar in structure to BIGLYCAN.
Cyclin-Dependent Kinase Inhibitor p27
A cyclin-dependent kinase inhibitor that coordinates the activation of CYCLIN and CYCLIN-DEPENDENT KINASES during the CELL CYCLE. It interacts with active CYCLIN D complexed to CYCLIN-DEPENDENT KINASE 4 in proliferating cells, while in arrested cells it binds and inhibits CYCLIN E complexed to CYCLIN-DEPENDENT KINASE 2.
Myocytes, Smooth Muscle
Cells, Cultured
Action of chondroitinases. II. Numerical calculation of the degree of multiple attack. (1/149)
Further investigation was carried out on the action patterns of two chondroitinase-AC [EC 4.2.2.5.] preparations obtained from Arthrobacter aurescens and Flavobacterium heparinum. To infer the action patterns of the chondroitinases, we proposed a new method for the calculation of the degree of multiple attack, based on the concept established by Robyt and French ((1967) Arch. Biochem. Biophys. 122, 8-16). It was shown that the degree of multiple attack (DM) is represented by the ratio of the initial velocity of number-average degree of scission to that of viscosity-average degree of scission. By this method, DM for A-Chase was estimated to be 3.03 and for F-chase, 1.31. (+info)Substrate specificity studies of Flavobacterium chondroitinase C and heparitinases towards the glycosaminoglycan--protein linkage region. Use of a sensitive analytical method developed by chromophore-labeling of linkage glycoserines using dimethylaminoazobenzenesulfonyl chloride. (2/149)
Bacterial chondroitinases and heparitinases are potentially useful tools for structural studies of chondroitin sulfate and heparin/heparan sulfate. Substrate specificities of Flavobacterium chondroitinase C, as well as heparitinases I and II, towards the glycosaminoglycan-protein linkage region -HexA-HexNAc-GlcA-Gal-Gal-Xyl-Ser (where HexA represents glucuronic acid or iduronic acid and HexNAc represents N-acetylgalactosamine or N-acetylglucosamine) were investigated using various structurally defined oligosaccharides or oligosaccharide-serines derived from the linkage region. In the case of oligosaccharide-serines, they were labeled with a chromophore dimethylaminoazobenzenesulfonyl chloride (DABS-Cl), which stably reacted with the amino group of the serine residue and rendered high absorbance for microanalysis. Chondroitinase C cleaved the GalNAc bond of the pentasaccharides or hexasaccharides derived from the linkage region of chondroitin sulfate chains and tolerated sulfation of the C-4 or C-6 of the GalNAc residue and C-6 of the Gal residues, as well as 2-O-phosphorylation of the Xyl residue. In contrast, it did not act on the GalNAc-GlcA linkage when attached to a 4-O-sulfated Gal residue. Heparitinase I cleaved the innermost glucosaminidic bond of the linkage region oligosaccharide-serines of heparin/heparan sulfate irrespective of substitution by uronic acid, whereas heparitinase II acted only on the glucosaminidic linkages of the repeating disaccharide region, but not on the innermost glucosaminidic linkage. These defined specificities of chondroitinase C, as well as heparitinases I and II, will be useful for preparation and structural analysis of the linkage oligosaccharides. (+info)Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. (3/149)
Radioactivity was significantly incorporated from ascorbate 2-[35S]sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. The extent of incorporation was comparable with that from inorganic [35S]sulfate. The radioactive chondroitin sulfate formed from ascorbate 2-[35S]sulfate gave two radioactive disaccharides on chondroitinase-ABC [EC 4.2.2.4] digestion. The incorporation was markedly decreased by inorganic sulfate. The time course of incorporation from ascorbate 2-[35S]sulfate and inorganic [35S]sulfate into chondroitin sulfate and the constituent disaccharides suggest that the incorporation rates from the two radioactive substances are different. (+info)Sulphation heterogeneity in the trisaccharide (GalNAcSbeta1, 4GlcAbeta1,3GalNAcS) isolated from the non-reducing terminal of human aggrecan chondroitin sulphate. (4/149)
We report here the isolation and sulphation isomer analyses of trisaccharides GalNAcS(beta1,4)GlcA(beta1,3)GalNAcS (in which S indicates sulphate) derived from the non-reducing termini of aggrecan chondroitin sulphate. Rat chondrosarcoma and human aggrecans were digested for 1 h at 37 degrees C with 30 micro-units of endo-chondroitinase ABC per microgram of chondroitin sulphate, and trisaccharides were isolated from the digests by ToyoPearl HW40S gel-filtration chromatography. Four trisaccharide species were identified; their sulphation isomer compositions, as determined by digestion with chondroitinase ACII and fluorescence-based ion-exchange HPLC, were GalNAc4Sbeta1,4GlcAbeta1,3GalNAc4S, GalNAc4Sbeta1,4GlcAbeta1,3GalNAc6S, GalNAc4,6Sbeta1,4GlcAbeta1, 3GalNAc4S and GalNAc4,6Sbeta1,4GlcAbeta1,3GalNAc6S. The abundances of such sequences in chondroitin sulphate on aggrecan from normal (foetal to 72 years of age) and from osteoarthritic human knee cartilages were also established. The results showed that non-reducing terminal GalNAc4S or GalNAc4,6S can be linked to either a 4-sulphated or a 6-sulphated disaccharide, suggesting that the sulphation of the last disaccharide might not have a direct effect on the specificity of chondroitin sulphate terminal GalNAc sulphotransferases. Furthermore, for each aggrecan preparation examined, the 4S-to-6S ratio of all chain interior disaccharides was equivalent to that in the last repeating disaccharides at the non-reducing terminus, suggesting that neither chondroitin 4-sulphotransferase nor chondroitin 6-sulphotransferase shows preferential activity near the chain terminus. (+info)Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum. (5/149)
In medium supplemented with chondroitin sulfate, Flavobacterium heparinum synthesizes and exports two chondroitinases, chondroitinase AC (chondroitin AC lyase; EC 4.2.2.5) and chondroitinase B (chondroitin B lyase; no EC number), into its periplasmic space. Chondroitinase AC preferentially depolymerizes chondroitin sulfates A and C, whereas chondroitinase B degrades only dermatan sulfate (chondroitin sulfate B). The genes coding for both enzymes were isolated from F. heparinum and designated cslA (chondroitinase AC) and cslB (chondroitinase B). They were found to be separated by 5.5 kb on the chromosome of F. heparinum, transcribed in the same orientation, but not linked to any of the heparinase genes. In addition, the synthesis of both enzymes appeared to be coregulated. The cslA and cslB DNA sequences revealed open reading frames of 2,103 and 1,521 bp coding for peptides of 700 and 506 amino acid residues, respectively. Chondroitinase AC has a signal sequence of 22 residues, while chondroitinase B is composed of 25 residues. The mature forms of chondroitinases AC and B are comprised of 678 and 481 amino acid residues and have calculated molecular masses of 77,169 and 53,563 Da, respectively. Truncated cslA and cslB genes have been used to produce active, mature chondroitinases in the cytoplasm of Escherichia coli. Partially purified recombinant chondroitinases AC and B exhibit specific activities similar to those of chondroitinases AC and B from F. heparinum. (+info)A fingerprinting method for chondroitin/dermatan sulfate and hyaluronan oligosaccharides. (6/149)
A previously published method for the analysis of glycosaminoglycan disaccharides by high pH anion exchange chromatography (Midura,R.J., Salustri,A., Calabro,A., Yanagishita,M. and Hascall,V.C. (1994), Glycobiology,4, 333-342) has been modified and calibrated for chondroitin and dermatan sulfate oligosaccharides up to hexasaccharide in size and hyaluronan oligosaccharides up to hexadecasaccharide. For hyaluronan oligosaccharides chain length controls elution position; however, for chondroitin and dermatan sulfate oligosaccharides elution times primarily depend upon the level of sulfation, although chain length and hence charge density plays a role. The sulfation position of GalNAc residues within an oligosaccharide is also important in determining its elution position. Compared to 4-sulfation a reducing terminal 6-sulfate retards elution; however, when present on an internal GalNAc residue it is the 4-sulfate containing oligosaccharide which elutes later. These effects allow discrimination between oligosaccharides differing only in the position of GalNAc sulfation. Using this simple methodology, a Dionex CarboPac PA-1 column with NaOH/NaCl eluents and detection by absorbance at 232 nm, a quantitative analytical fingerprint of a chondroitin/dermatan sulfate chain may be obtained, allowing a determination of the abundance of chondroitin sulfate, dermatan sulfate, and hyaluronan along with an analysis of structural features with a linear response to approximately 0.1 nmol. The method may readily be calibrated using either commercial disaccharides or the di- and tetrasaccharide products of a limit digest of commercial chondroitin sulfate by chondroitin ABC endolyase. Commercially available and freshly prepared shark, whale, bovine, and human cartilage chondroitin sulfates have been examined by this methodology and we have confirmed that freshly isolated shark cartilage CS contains significant amounts of the biologically important GlcA2Sbeta(1-3)GalNAc6S structure. (+info)Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection. (7/149)
Respiratory syncytial virus (RSV) is an important human respiratory pathogen, particularly in infants. Glycosaminoglycans (GAGs) have been implicated in the initiation of RSV infection of cultured cells, but it is not clear what type of GAGs and GAG components are involved, whether the important GAGs are on the virus or the cell, or what the magnitude is of their contribution to infection. We constructed and rescued a recombinant green fluorescent protein (GFP)-expressing RSV (rgRSV) and used this virus to develop a sensitive system to assess and quantify infection by flow cytometry. Evaluation of a panel of mutant Chinese hamster ovary cell lines that are genetically deficient in various aspects of GAG synthesis showed that infection was reduced up to 80% depending on the type of GAG deficiency. Enzymatic removal of heparan sulfate and/or chondroitin sulfate from the surface of HEp-2 cells also reduced infection, and the removal of both reduced infection even further. Blocking experiments in which RSV was preincubated with various soluble GAGs revealed the relative blocking order of: heparin > heparan sulfate > chondroitin sulfate B. Iduronic acid is a component common to these GAGs. GAGs that do not contain iduronic acid, namely, chondroitin sulfate A and C and hyaluronic acid, did not inhibit infection. A role for iduronic acid-containing GAGs in RSV infection was confirmed by the ability of basic fibroblast growth factor to block infection, because basic fibroblast growth factor binds to GAGs containing iduronic acid. Pretreatment of cells with protamine sulfate, which binds and blocks GAGs, also reduced infection. In these examples, infection was reduced by pretreatment of the virus with soluble GAGs, pretreatment of the cells with GAG-binding molecules, pretreatment of the cells with GAG-destroying enzymes or in cells genetically deficient in GAGs. These results establish that the GAGs involved in RSV infection are present on the cell rather than on the virus particle. Thus, the presence of cell surface GAGs containing iduronic acid, like heparan sulfate and chondroitin sulfate B, is required for efficient RSV infection in cell culture. (+info)Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates. (8/149)
Sulfated glycosaminoglycans were isolated from 23 species of 13 phyla of invertebrates and characterized by their electrophoretic migration in three different buffer systems coupled with enzymatic degradation using bacterial heparinase, heparitinases and chondroitinase AC. Heparan sulfate is a ubiquitous compound present in all species analyzed whereas chondroitin sulfate was present in 20 species and heparin-like compounds in 12 species of the invertebrates. The heparin-like compounds were purified from the echinoderm Mellita quinquisperforata (sand dollar) and the crustacean Ucides cordatus (crab) with anticoagulant activities of 60 and 52 IU/mg, respectively. Degradation of these heparins with heparinase produced significant amounts of the trisulfated disaccharide typical of mammalian heparins. This was confirmed by 13C-NMR spectroscopy of the crab heparin. An updated phylogenetic tree of the distribution of sulfated glycosaminoglycans in the animal kingdom is also presented. (+info)
CAZy - PL8
Chondroitin ABC lyase - Wikipedia
HPLC Glycosaminoglycan Analysis in Patients with Graves Disease | Clinical Science | Portland Press
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List of MeSH codes (D08)
... chondroitin lyases MeSH D08.811.520.241.700.350.500.500 - chondroitin abc lyase MeSH D08.811.520.241.700.512 - heparin lyase ... polysaccharide-lyases MeSH D08.811.520.241.700.350 - chondroitinases and chondroitin lyases MeSH D08.811.520.241.700.350.500 - ... tyrosine phenol-lyase MeSH D08.811.520.232.300 - amidine-lyases MeSH D08.811.520.232.300.200 - adenylosuccinate lyase MeSH ... ammonia-lyases MeSH D08.811.520.232.400.200 - aspartate ammonia-lyase MeSH D08.811.520.232.400.350 - ethanolamine ammonia-lyase ...
Chondroitinase
... an enzyme Chondroitin ABC lyase, an enzyme This set index page lists enzyme articles associated with the same name. If an ...
CHNB
Ontario Chondroitin B lyase, an enzyme This disambiguation page lists articles associated with the title CHNB. If an internal ...
List of EC numbers (EC 4)
... pectate lyase EC 4.2.2.3: poly(b-D-mannuronate) lyase EC 4.2.2.4: chondroitin ABC lyase EC 4.2.2.5: chondroitin AC lyase EC 4.2 ... chondroitin B lyase EC 4.2.2.20: chondroitin-sulfate-ABC endolyase EC 4.2.2.21: chondroitin-sulfate-ABC exolyase EC 4.2.2.22: ... pectin lyase EC 4.2.2.11: poly(a-L-guluronate) lyase EC 4.2.2.12: xanthan lyase EC 4.2.2.13: exo-(1-4)-α-D-glucan lyase EC 4.2. ... oligogalacturonide lyase EC 4.2.2.7: heparin lyase EC 4.2.2.8: heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC ...
Chondroitin ABC lyase
... (EC 4.2.2.20 EC 4.2.2.21, chondroitinase, chondroitin ABC eliminase, chondroitinase ABC) is an enzyme ... Chondroitin+ABC+lyase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... with systematic name chondroitin ABC lyase. This enzyme catalyses the following chemical reaction Eliminative degradation of ... chondroitin 6-sulfate and dermatan sulfate. Following a spinal cord injury, this enzyme can be used to erode scar tissue that ...
Chondroitin-sulfate-ABC exolyase
... chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ... specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin- ... "Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially ... ABC exoeliminase, chondroitin sulfate ABC exolyase, and ChS ABC lyase II. Yamagata T, Saito H, Habuchi O, Suzuki S (1968). " ...
Chondroitin sulfate ABC lyase
... may refer to: Chondroitin ABC lyase, an enzyme Chondroitin-sulfate-ABC exolyase, an enzyme ... an enzyme This disambiguation page lists articles associated with the title Chondroitin sulfate ABC lyase. If an internal link ...
Chondroitin B lyase
In enzymology, a chondroitin B lyase (EC 4.2.2.19) is an enzyme that catalyzes the chemical reaction Eliminative cleavage of ... The systematic name of this enzyme class is chondroitin B lyase. Other names in common use include chondroitinase B, ChonB, and ... V, Sasisekharan R, Cygler M (2004). "The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides ... characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum". Biochem. J. 312 (Pt 2 ...
Chondroitin AC lyase
... chondroitin sulfate lyase, chondroitin AC eliminase, chondroitin AC lyase, chondroitinase AC, and ChnAC. As of late 2007, 11 ... In enzymology, a chondroitin AC lyase (EC 4.2.2.5) is an enzyme that catalyzes the chemical reaction Eliminative degradation of ... The systematic name of this enzyme class is chondroitin AC lyase. Other names in common use include chondroitinase (ambiguous ... "Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced ...
Heparin
The lyases have mainly been used in heparin/HS studies. The bacterium produces three lyases, heparinases I (EC 4.2.2.7), II (no ... The most prevalent impurity in heparin is dermatan sulfate (DS), also known as chondroitin sulfate B. The building-block of DS ... The lyases cleave heparin/HS by a beta elimination mechanism. This action generates an unsaturated double bond between C4 and ... Danaparoid, a mixture of heparan sulfate, dermatan sulfate, and chondroitin sulfate can be used as an anticoagulant in patients ...
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對於肝素誘發的血小板減少症患者,可以使用不含有肝素或肝素片段的Danaparoid(英语:Danaparoid):它是硫酸肝素、硫酸皮膚素(英语:dermatan sulfate)與硫酸軟骨素(英语:chondroitin sulfate)的混合物,因為 ... Specificity studies on the heparin lyases from Flavobacterium heparinum. Biochemistry. 1993, 32 (32): 8140-8145. PMID 8347612. ... Examination of the substrate specificity of heparin and heparan sulfate lyases. Biochemistry. 1990, 29 (10): 2611-2617
COX-2 inhibitor
Lyase (EC 4). *4.1 Dopa decarboxylase. *4.2 Carbonic anhydrase. Miscellaneous. *Steroidogenesis inhibitor ...
Superoxide dismutase
EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list). *EC7 Translocases (list) ...
Հեպարին - Վիքիպեդիա՝ ազատ հանրագիտարան
1990)։ «Examination of the substrate specificity of heparin and heparan sulfate lyases»։ Biochemistry 29 (10): 2611-2617։ PMID ... Flengsrud R (2016)։ «Disaccharide analysis of chondroitin and heparin from farmed Atlantic salmon»։ Glycoconjugate Journal 33 ( ... 1993)։ «Specificity studies on the heparin lyases from Flavobacterium heparinum»։ Biochemistry 32 (32): 8140-8145։ PMID 8347612 ...
List of EC numbers (EC 3)
The activity is most probably attributable to EC 4.4.1.21, S-ribosylhomocysteine lyase EC 3.13.1.3: 2'-hydroxybiphenyl-2- ... unsaturated chondroitin disaccharide hydrolase EC 3.2.1.181: galactan endo-beta-1,3-galactanase EC 3.2.1.182: 4-hydroxy-7- ... citrate lyase deacetylase EC 3.1.2.17: (S)-methylmalonyl-CoA hydrolase EC 3.1.2.18: ADP-dependent short-chain-acyl-CoA ...
List of EC numbers (EC 2)
... lyase) S-acetyltransferase EC 2.3.1.50: serine C-palmitoyltransferase EC 2.3.1.51: 1-acylglycerol-3-phosphate O-acyltransferase ... chondroitin 4-sulfotransferase EC 2.8.2.6: choline sulfotransferase EC 2.8.2.7: UDP-N-acetylgalactosamine-4-sulfate ... chondroitin 6-sulfotransferase EC 2.8.2.18: cortisol sulfotransferase EC 2.8.2.19: triglucosylalkylacylglycerol ...
L-DOPA
3-hydroxy-3-methylglutaryl-CoA lyase. TRYPTOPHAN→. *Indoleamine 2,3-dioxygenase/Tryptophan 2,3-dioxygenase ... Chondroitin sulfate. *Cod liver oil. *Copper gluconate. *Creatine/Creatine supplements. *Dietary fiber ...
Taurine
The cystathionine is then converted to hypotaurine by the sequential action of three enzymes: cystathionine gamma-lyase, ...
Chondroitin ABC lyase - Wikipedia
Chondroitin ABC lyase (EC 4.2.2.20 EC 4.2.2.21, chondroitinase, chondroitin ABC eliminase, chondroitinase ABC) is an enzyme ... Chondroitin+ABC+lyase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... with systematic name chondroitin ABC lyase. This enzyme catalyses the following chemical reaction Eliminative degradation of ... chondroitin 6-sulfate and dermatan sulfate. Following a spinal cord injury, this enzyme can be used to erode scar tissue that ...
Chondroitin sulfate ABC lyase - Wikipedia
RCSB PDB - 2Q1F: Crystal structure of chondroitin sulfate lyase abc from bacteroides thetaiotaomicron wal2926
... chondroitin lyases AC) and beta-helix for DS (chondroitin lyases B); their catalytic mechanisms have been established at the ... The family of bacterial chondroitin lyases ABC comprises such enzymes. They can degrade both chondroitin sulfate (CS) and ... Composite active site of chondroitin lyase ABC accepting both epimers of uronic acid.. Shaya, D., Hahn, B.S., Bjerkan, T.M., ... Crystal structure of chondroitin sulfate lyase abc from bacteroides thetaiotaomicron wal2926. *DOI: 10.2210/pdb2Q1F/pdb ...
RCSB PDB - 1RWH: Crystal structure of Arthrobacter aurescens chondroitin AC lyase in complex with chondroitin tetrasaccharide
Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate ... Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate ... Crystal structure of Arthrobacter aurescens chondroitin AC lyase in complex with chondroitin tetrasaccharide. *DOI: 10.2210/ ... Like other chondroitin AC lyases, it is capable of cleaving hyaluronan. We have determined the three-dimensional crystal ...
Chondroitin Lyase from a Marine Arthrobacter sp. MAT3885 for the Production of Chondroitin Sulfate Disaccharides | SpringerLink
Chondroitin sulfate (CS) saccharides from cartilage tissues have potential application in medicine or as dietary supplements ... Chondroitin sulfate Chondroitin sulfate lyase Marine Arthrobacter sp. This is a preview of subscription content, log in to ... Chondroitin Lyase from a Marine Arthrobacter sp. MAT3885 for the Production of Chondroitin Sulfate Disaccharides. ... As the recombinant chondroitin sulfate lyase (designated as ChoA1R) degraded chondroitin sulfate efficiently compared to a ...
HKU Scholars Hub: Factors underlying improved efficacy of chondroitin sulphate lyases in overcoming chondroitin sulphate...
Conference Paper: Factors underlying improved efficacy of chondroitin sulphate lyases in overcoming chondroitin sulphate ... Factors underlying improved efficacy of chondroitin sulphate lyases in overcoming chondroitin sulphate barriers for nerve ... Factors underlying improved efficacy of chondroitin sulphate lyases in overcoming chondroitin sulphate barriers for nerve ...
Chondroitin ABC lyase from Bacteroides thetaiotaomicron, Recombinant(EC 4.2.2.20) - Creative Enzymes
... chondroitin ABC lyase; chondroitin sulfate ABC lyase; ChS ABC lyase; chondroitin sulfate ABC endoeliminase; chondroitin sulfate ... Chondroitin ABC lyase from Bacteroides thetaiotaomicron, Recombinant. Chondroitin ABC lyase from Bacteroides thetaiotaomicron, ... Chondroitin sulfate A (CS-A), chondroitin sulfate B (CS-B), chondroitin sulfate C (CS-C), chondroitin (C), and H ... Cystathionine-β-Synthase/Lyase Industrial Enzyme Production Enzyme Engineering and Modification Creatinase/Creatininase/ ...
Sodium in the structure of Crystal Structure of Arthrobacter Aurescens Chondroitin Ac Lyase (pdb 1rwc)
Frontiers | The Complete Genome Sequence of the Fish Pathogen Tenacibaculum maritimum Provides Insights into Virulence...
... a sphingomyelinase and a chondroitin AC lyase which activities were demonstrated in vitro. ... a sphingomyelinase and a chondroitine AC lyase which activities were demonstrated in vitro. ... Chondroitin AC Lyase In Vitro Activity. The native chondroitin AC lyase activity of T. maritimum was assayed in vitro according ... Chondroitin AC Lyase and Sphingomyelinase Cloning, Expression, and Enzymatic Activity. The genes encoding the chondroitin AC ...
HKU Scholars Hub: Combinatorial use of chondroitin sulphate lyase subtypes in the treatment of nerve injury
chondroitin sulphate lyases. -. dc.title. Combinatorial use of chondroitin sulphate lyase subtypes in the treatment of nerve ... Conference Paper: Combinatorial use of chondroitin sulphate lyase subtypes in the treatment of nerve injury. *Show simple item ... Chondroitin sulphate ABC lyases (ChABC) from Proteus vulgaris have been exploited to cleave CS moieties in the injured ... Chondroitin sulphate ABC lyases (ChABC) from Proteus vulgaris have been exploited to cleave CS moieties in the injured ...
Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum | UBC Chemistry
Chondroitin AC lyase from Flavobacterium heparinum degrades chondroitin sulfate glycosaminoglycans via an elimination mechanism ... Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum. ... Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum. ... Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum ...
algL - Alginate lyase precursor - Azotobacter vinelandii - algL gene & protein
IPR022859 Alginate_lyase. IPR008397 Alginate_lyase_dom. IPR008929 Chondroitin_lyas. Pfami. View protein in Pfam. PF05426 ... Alginate lyase1 Publication. ,p>Manually curated information that is based on statements in scientific articles for which there ... Belongs to the polysaccharide lyase 5 family.UniRule annotation. Manual assertion according to rulesi ... Poly(beta-D-mannuronate) lyaseUniRule annotation. Manual assertion according to rulesi ...
A histological and histochemical study on glycosaminoglycans in epiphysial cartilage of osteochondrodysplasia rat (OCD/OCD)
Age-Related Changes in Populations of Aortic Glycosaminoglycans | Arteriosclerosis, Thrombosis, and Vascular Biology
Chondroitin AC lyase (EC 4.2.2.5) from Arthrobacter aurescens and chondroitin ABC lyase (EC 4.2.2.4) from Proteus vulgaris were ... Digestion with chondroitin AC lyase yielded proportions of isomeric chondroitin 4-sulfate and chondroitin 6-sulfate (Fig 3D⇑) ... Glycosaminoglycans (100 μg) were incubated with 10 mU chondroitin AC lyase or chondroitin ABC lyase in 100 μL of 50 mmol/L Tris ... After incubation of the dermatan sulfate+chondroitin 4/6-sulfate with chondroitin AC lyase (see "Methods"), the mixtures were ...
CATH Superfamily 1.50.10.100
Fibrillin: evidence that chondroitin sulphate proteoglycans are components of microfibrils and associate with newly synthesised...
Microfibril integrity was disrupted by chondroitinase ABC lyase and chondroitinase … ... Microfibril integrity was disrupted by chondroitinase ABC lyase and chondroitinase AC lyase, but not by keratanase or ... Fibrillin: evidence that chondroitin sulphate proteoglycans are components of microfibrils and associate with newly synthesised ... These experiments indicate that chondroitin sulphate proteoglycans associate with fibrillin and contribute to microfibril ...
Sodium in the structure of Crystal Structure of Chondroitin Abc Lyase I From Proteus Vulgaris At 1.9 Angstroms Resolution (pdb...
Crystal Structure of Chondroitin Abc Lyase I From Proteus Vulgaris At 1.9 Angstroms Resolution ... The binding sites of Sodium atom in the structure of Crystal Structure of Chondroitin Abc Lyase I From Proteus Vulgaris At 1.9 ... Sodium in the structure of Crystal Structure of Chondroitin Abc Lyase I From Proteus Vulgaris At 1.9 Angstroms Resolution (pdb ...
The structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-Dependent Catalytic...
The structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-Dependent Catalytic ... The structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-Dependent Catalytic ... The structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-Dependent Catalytic ...
MEDLINE - Resultado p gina 1
Polysaccharide-Lyases); EC 4.2.2.- (heparinase II); EC 4.2.2.7 (Heparin Lyase); EC 4.2.2.8 (heparitinsulfate lyase). ... Highly sensitive ratiometric detection of heparin and its oversulfated chondroitin sulfate contaminant by fluorescent peptidyl ... 0 (Heparin, Low-Molecular-Weight); 0 (Nadroparin); 9005-49-6 (Heparin); EC 4.2.2.7 (Heparin Lyase); T2I5UM75DN (Nitrous Acid). ... 0 (Anticoagulants); 0 (Fluorescent Dyes); 0 (Peptides); 0 (Pyrenes); 9005-49-6 (Heparin); 9007-28-7 (Chondroitin Sulfates); ...
Purification and characterization of novel chondroitin ABC and AC...: Ingenta Connect
Pedobacter heparinus (Payza and Korn) Stein et al. ATCC ® 13125&tr
Neuroglycan C is a novel midkine receptor involved in process elongation of oligodendroglial precursor-like cells.
Chondroitin ABC Lyase / metabolism. Chondroitin Sulfates / chemistry. Cytokines / metabolism. Epidermal Growth Factor / ... 9007-28-7/Chondroitin Sulfates; EC 4.2.2.20/Chondroitin ABC Lyase ... Neuroglycan C lacking chondroitin sulfate chains was eluted with 0.5 m NaCl as a major fraction from the column. We confirmed ... Among three functional domains of the extracellular part of neuroglycan C, the chondroitin sulfate attachment domain and acidic ...
Chondroitin sulfate-E mediates estrogen-induced osteoanabolism | Scientific Reports
Here, we show that estrogen-induced, osteoanabolic effects were mediated via enhanced production of chondroitin sulfate-E (CS-E ... A chondroitin sulfate and hyaluronic acid lyase with poor activity to glucuronyl 4,6-O-disulfated N-acetylgalactosamine (E-type ... Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: ... Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in co-operation with chondroitin N- ...
Polysaccharide lyase family 8-like, C-terminal (IPR011071) | InterPro | EMBL-EBI
This entry represents the C-terminal domain of hyaluronate lyase, chondroitin AC lyase, chondroitin ABC lyase and xanthan lyase ... Structures of Streptococcus pneumoniae hyaluronate lyase in complex with chondroitin and chondroitin sulfate disaccharides. ... the C-terminal domain of chondroitin ABC lyase (EC:4.2.2.4) [PMID: 12706721], and the C-terminal domain of xanthan lyase (EC: ... Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes.. J. Mol. Biol. ...
UniProt: A0A1G9CQV0 9RHOB
KEGG ENZYME: 4.2.2.19
The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic ... Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum. ... This is the only lyase that is known to be specific for dermatan sulfate as substrate. The minimum substrate length required ... Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes ...
TNO Repository search for: subject:'Receptors, Thrombin'
... chondroitin ABC lyase, 9024-13-9; thrombin, 9002-04-4; thrombomodulin, 112049-68-0; urokinase, 139639-24-0; Chondroitin Lyases ... chondroitin 4 sulfate · chondroitin abc lyase · glycosaminoglycan · mutant protein · thrombomodulin · urokinase · dissociation ... Chondroitin Lyases · Dose-Response Relationship, Drug · Glycosaminoglycans · Glycosylation · Human · Receptors, Cell Surface · ... The free glycosarninoglycans obtained by alkaline treatment of TM or chondroitin sulphate A also accelerated the inactivation ...
EC 4.2.99.6
MEDLINE - Resultado p gina 1
0 (Aggrecans); 0 (RNA, Messenger); EC 4.2.2.20 (Chondroitin ABC Lyase). [Em] M s de entrada:. 1712. ... The administration of chondroitinase ABC degrades these chondroitin sulfate proteoglycans and improves functional recovery ... CONCLUSIONS: Significant upregulation of chondroitin sulfate proteoglycans and other extracellular matrix components ... and quantitative immunohistochemical analyses were performed to determine levels of chondroitin sulfate proteoglycans and ...
GlycosaminoglycansSulfatesProteoglycansProteoglycanAlginateChondroitinaseEnzymeEnzymesGlycoside HydrolasesHyaluronate lyaseOligosaccharidesHeparan sulfate and chondroitin sulfateHeparinumCharacterizationHyaluronan and chondroitinAnnotationProteinsDisaccharideDisaccharidesChainsUlvan lyasesProteusChromatographyProteinArthrobacterExolyase
Glycosaminoglycans7
- They can degrade both chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans at the nonreducing end of either glucuronic acid (CS) or its epimer iduronic acid (DS) by a beta-elimination mechanism, which commences with the removal of the C-5 proton from the uronic acid. (rcsb.org)
- Baici A, Horler D, Moser B, Hofer HO, Fehr K, Wagenhauser FJ (1992) Analysis of glycosaminoglycans in human serum after oral-administration of chondroitin sulfate. (springer.com)
- Chondroitin AC lyase from Flavobacterium heparinum degrades chondroitin sulfate glycosaminoglycans via an elimination mechanism resulting in disaccharides or oligosaccharides with Delta4,5-unsaturated uronic acid residues at their nonreducing end. (ubc.ca)
- The complex carbohydrate macromolecules named glycosaminoglycans are represented by heparin, heparan sulfate, dermatan sulfate, chondroitin sulfates, hyaluronic acid and keratan sulfate and by their derivatives. (unipv.it)
- Degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. (expasy.org)
- Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans after proteolysis and electrophoresis. (scielo.br)
- The tethered WG can be released by addition of exogenous heparan sulfate and chondroitin sulfate glycosaminoglycans. (rupress.org)
Sulfates3
- Bali JP, Cousse H, Neuzil E (2001) Biochemical basis of the pharmacologic action of chondroitin sulfates on the osteoarticular system. (springer.com)
- Characterization of oligosaccharides from the chondroitin/dermatan sulfates. (genome.jp)
- Chondroitin sulfates (CS) 1 are complex polysaccharides present at cell surfaces and in extracellular matrices. (mcponline.org)
Proteoglycans15
- Chondroitin sulphate (CS) proteoglycans are up-regulated in the extracellular matrix of injured nerves and constitute barriers to axonal re-growth. (hku.hk)
- These experiments indicate that chondroitin sulphate proteoglycans associate with fibrillin and contribute to microfibril assembly. (nih.gov)
- Decorin is a family member of the small leucine-rich chondroitin/dermatan sulfate proteoglycans and is present in the extracellular matrix (ECM) of a variety of tissues and cell types. (ahajournals.org)
- Brevican is a nervous system-specific chondroitin sulfate proteoglycan that belongs to the aggrecan family and is one of the most abundant chondroitin sulfate proteoglycans in adult brain. (jneurosci.org)
- Lecticans are a family of chondroitin sulfate proteoglycans (CSPGs) characterized by the presence of an N-terminal hyaluronan-binding domain and a C-terminal lectin-like domain. (jneurosci.org)
- Vertebrates produce various chondroitin sulfate proteoglycans (CSPGs) that are important structural components of cartilage and other connective tissues. (mcponline.org)
- The proteoglycans appeared as extended molecules with a beaded structure, representing the chondroitin sulphate chains collapsed around the protein core. (biochemj.org)
- Among these, HSPGs are composed of glucuronic acid-N-acetylglucosamine (GlcA-GlcNAc) repeating disaccharide units, whereas chondroitin sulfate (CS) proteoglycans contain glucuronic acid-N-acetylgalactosamine (GalNAc-GlcA) repeats. (biologists.org)
- Fingerprint Dive into the research topics of 'Chromaffin granule and PC12 cell chondroitin sulfate proteoglycans and their relation to chromogranin A'. Together they form a unique fingerprint. (elsevier.com)
- Digestion of 125I-labeled proteoglycans with heparitin-sulfate lyase and chondroitin ABC lyase yielded core proteins of approximately 93 kDa, approximately 85 kDa and approximately 38 kDa. (kuleuven.be)
- Comparison with single enzyme digestions identified the 93-kDa and 85-kDa cores as components of hybrid proteoglycans that carried both heparan-sulfate and chondroitin-sulfate chains. (kuleuven.be)
- Preparations enriched in heparan sulfate or in heparan-sulfate/chondroitin-sulfate hybrid proteoglycans were obtained by N-desulfation and ion-exchange chromatography. (kuleuven.be)
- Hybrid proteoglycans accounting for the bulk of the chondroitin-sulfate and nearly half of the heparan-sulfate residues of the proteoglycans showed a similar polydispersity of heparan-sulfate chain sizes as found in proteoglycans that carried only, or predominantly, heparan-sulfate chains. (kuleuven.be)
- Analysis of 125I-labeled proteoglycans suggested that typical hybrid proteoglycans were composed of a 85-kDa core protein that carries a single chondroitin-sulfate chain and a single heparan-sulfate chain of variable length. (kuleuven.be)
- Peptido-keratan sulphate fragments were isolated from the nucleus pulposus of bovine intervertebral discs (2-year-old animals) after digestion with chondroitin ABC lyase followed by digestion with diphenylcarbamoyl chloride-treated trypsin of A1D1 proteoglycans and gel-permeation chromatography on Sepharose CL-6B. (lancs.ac.uk)
Proteoglycan9
- 5. The method, according to claim 1, further comprising administering to the nerve at least one chondroitin sulfate proteoglycan-degrading enzyme. (freepatentsonline.com)
- 6. The method according to claim 5, wherein the chondroitin sulfate proteoglycan-degrading enzyme is selected from the group consisting of chondroitinase ABC, chondroitinase A, chondroitinase C, and chondroitinase AC. (freepatentsonline.com)
- This distribution was not changed by prior chondroitin AC lyase digestion of the proteoglycan. (biochemj.org)
- 20%) of the proteoglycan monomers that lost their beaded structure and appeared with the chondroitin sulphate chains projecting from the protein core. (biochemj.org)
- Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. (expasy.org)
- Finally, treatment of plasma membranes with either proteases or chondroitinase ABC completely abrogated membrane binding of DBP, suggesting that the protein binds to a chondroitin sulfate proteoglycan. (jimmunol.org)
- Thus, the type III TGF-beta receptor has the properties of a membrane proteoglycan that carries heparan and chondroitin sulfate glycosaminoglycan chains. (umassmed.edu)
- The [ 35 S]sulphate-labelled material yielded large trypsin-resistant fragments and contained O-linked glycans but was not affected by digestion with chondroitin ABC lyase or heparan sulphate lyase, suggesting that it is a mucin rather than a proteoglycan. (lu.se)
- Previous studies have shown that the matrix chondroitin sulfate proteoglycan, versican, exhibits a similar pattern of expression in the embryonic joint rudiment of chick and mouse suggesting conserved function during joint development. (docme.ru)
Alginate19
- Origin and Diversity of Alginate Lyases of Families PL-5 and -7 in Sphingomonas sp. (asm.org)
- strain A1 has three endotype alginate lyases (A1-I, A1-II [family PL-7], and A1-III [family PL-5]), each of which is encoded by a single gene. (asm.org)
- In addition to those of these lyases, a gene (the A1-II′ gene) showing significant identity with the A1-II gene was present in the bacterial genome and coded for an alginate lyase with broad substrate specificity. (asm.org)
- Alginate lyase depolymerizes alginate through the β-elimination reaction. (asm.org)
- A large number of alginate lyases, from sources ranging from bacteria to marine animals, have been characterized ( 32 ), although little information on the diversity and evolution of alginate lyases has been accumulated. (asm.org)
- Most alginate lyases are classified into two families, PL-5 and -7. (asm.org)
- Generally, alginate lyases in families PL-5 and -7 preferably depolymerize poly(M) and poly(G), respectively, though many enzymes can cleave M-G or G-M bonds. (asm.org)
- In order to clarify the structure and function of alginate lyases, we have studied the metabolism of alginate in cells of Sphingomonas sp. (asm.org)
- The bacterium incorporates the macromolecule (alginate) through a "superchannel," consisting of a pit formed on the cell surface and a pit-dependent ABC transporter ( 13 , 22 ), and depolymerizes the polymer into its constituent monosaccharides through concerted reactions catalyzed by three intracellular endotype alginate lyases (A1-I, A1-II, and A1-III) and an exotype alginate lyase (A1-IV) ( 8 , 36 ). (asm.org)
- The three endotype alginate lyases are encoded by a single gene ( 21 ), and precursor protein A1-I is autocatalytically processed into A1-II and A1-III ( 14 ). (asm.org)
- Tomato strain DC3000 ( 6 ) have both family PL-5 and -7 alginate lyases, although their genes are separately located in the bacterial genomes. (asm.org)
- Therefore, we propose that A1-II and A1-III encoded by the A1-I gene are the original alginate lyases of families PL-7 and -5, respectively, and that the A1-II and A1-III genes that were derived from the A1-I gene independently evolved into various genes belonging to families PL-7 and -5 through duplication, modification, and translocation. (asm.org)
- As the first step to confirm this hypothesis, we report here the molecular diversity and evolution of alginate lyases in Sphingomonas sp. (asm.org)
- Occurrence of a gene homologous to the alginate lyase A1-I gene in Sphingomonas sp. (asm.org)
- The present invention relates to the identification, production and use of thermostable alginate lyase enzymes that can be used to partially degrade alginate to yield oligosaccharides or to give complete degradation of alginate to yield (unsaturated) mono-uronates. (freepatentsonline.com)
- 1. A recombinant construct comprising a DNA sequence comprising a coding region for a thermostable alginate lyase enzyme. (freepatentsonline.com)
- 6. An isolated protein comprising an enzymatically active alginate lyase sequence having a thermostable alginate lyase activity. (freepatentsonline.com)
- 12. The isolated protein of claim 6 having a thermostable alginate lyase activity on alginate oligo/polysaccharides such that it preferentially cleaves M-G bonds and less preferentially G-G or M-M or G-M bonds. (freepatentsonline.com)
- 15. The isolated protein of claim 6 having a thermostable alginate lyase activity on alginate oligo/polysaccharides such that it preferentially cleaves M-M in an Exo-fashion and yielding preferentially monosugars. (freepatentsonline.com)
Chondroitinase6
- Chondroitin ABC lyase (EC 4.2.2.20 EC 4.2.2.21, chondroitinase, chondroitin ABC eliminase, chondroitinase ABC) is an enzyme with systematic name chondroitin ABC lyase. (wikipedia.org)
- Microfibril integrity was disrupted by chondroitinase ABC lyase and chondroitinase AC lyase, but not by keratanase or hyaluronidase. (nih.gov)
- Newly synthesised fibrillin could be labelled with 35S TransLabel, [3H]glucosamine or 35SO4 but its electrophoretic mobility was not influenced by treatment with chondroitinase ABC or AC lyase. (nih.gov)
- Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum. (genome.jp)
- The chondroitinase AC and B transconjugant strains, grown in heparin-only medium, yielded 20- and 13-fold increases, respectively, in chondroitinase AC and B expression, compared to wild-type F. heparinum grown in chondroitin sulfate A-only medium. (asm.org)
- CSPGs were enriched from human urine and cerebrospinal fluid samples by strong-anion-exchange chromatography, digested with chondroitinase ABC, a specific CS-lyase used to reduce the CS chain lengths and subsequently analyzed by nLC-MS/MS with a novel glycopeptide search algorithm. (mcponline.org)
Enzyme11
- This enzyme catalyses the following chemical reaction Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups This enzyme acts on chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate. (wikipedia.org)
- Chondroitin sulfate ABC lyase may refer to: Chondroitin ABC lyase, an enzyme Chondroitin-sulfate-ABC exolyase, an enzyme Chondroitin-sulfate-ABC endolyase, an enzyme This disambiguation page lists articles associated with the title Chondroitin sulfate ABC lyase. (wikipedia.org)
- ChoA1 was defined as an AC-type enzyme as it degraded chondroitin sulfate A, C, and hyaluronic acid. (springer.com)
- As the recombinant chondroitin sulfate lyase (designated as ChoA1R) degraded chondroitin sulfate efficiently compared to a benchmark enzyme, it may be used for the production of chondroitin sulfate disaccharides for the food industry or health-promoting products. (springer.com)
- One such enzyme is hyaluronate lyase, a Streptococcal surface enzyme that degrades hyaluronan and chondroitin, thereby helping to spread the bacteria throughout host tissues [ PMID: 14523022 ]. (ebi.ac.uk)
- Hyaluronate lyase ( EC:4.2.2.1 ) is a four-domain enzyme containing an N-terminal carbohydrate-binding domain, a spacer domain, a catalytic domain, and a C-terminal domain that modulates access to the catalytic cleft of the enzyme. (ebi.ac.uk)
- Based on the analysis of a large panel of experimentally characterized polysaccharide lyases, we examined the correlation of various enzyme properties with the three levels of the classification: fold, family and subfamily. (biochemj.org)
- The enzyme topology reveals a predominantly α-helical enzyme with a distorted (α/α) 3 barrel quite unlike the parallel β-helix displayed by other pectate lyases (Pel). (pnas.org)
- It was shown that ChnA is a 75-kDa enzyme degrading both chondroitin sulfate A and C and that ChnB is a 55-kDa enzyme degrading only chondroitin sulfate B ( 11 ). (asm.org)
- Erindi 47: Cloning and expression of a chondroitin lyase gene from the marine bacterium Arthrobacter MAT3885 and characterization of the recombinant enzyme. (matis.is)
- The lyase was overexpressed in Escherichia coli, and the purified recombinant enzyme depolymerised ulvan in an endolytic manner with a Kmof 0.77 mg/ml, and displayed optimum activity at 40 °C and pH 8. (chalmers.se)
Enzymes10
- The family of bacterial chondroitin lyases ABC comprises such enzymes. (rcsb.org)
- Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. (rcsb.org)
- Proteins containing this C-terminal domain consist of a group of secreted bacterial lyase enzymes capable of acting on a variety of substrates. (ebi.ac.uk)
- Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes. (ebi.ac.uk)
- In the present paper, a classification of polysaccharide lyases (the enzymes that cleave polysaccharides using an elimination instead of a hydrolytic mechanism) is shown thoroughly for the first time. (biochemj.org)
- PLs (polysaccharide lyases) are a group of enzymes (EC 4.2.2. (biochemj.org)
- Enzymes which catalyze the elimination of glucuronate residues from chondroitin A,B, and C or which catalyze the hydrolysis of sulfate groups of the 2-acetamido-2-deoxy-D-galactose 6-sulfate units of chondroitin sulfate. (bioportfolio.com)
- In addition to their role in the carbon cycle, polygalacturonic acid lyases are important virulence factors of plant pathogens, such as Erwinia chrysanthemi ( 3 ), whereas the enzymes from Pseudomonas fluorescens and Pseudomonas viridiflava are responsible for the majority of fresh fruit and vegetable spoilage ( 7 ). (pnas.org)
- The CAZy classification ( 5 ) describes 12 families of polysaccharide lyases with polygalacturonate-active enzymes found in families PL-1, 2, 3, 9, and 10. (pnas.org)
- Here we report the 1.3-Å resolution three-dimensional structure of the competent catalytic module of the polygalacturonic acid lyase Pel10A (Pel10Acm), from Cellvibrio japonicus, together with analysis of the activity of wild-type and mutant enzymes. (pnas.org)
Glycoside Hydrolases1
- In contrast to the 87 sequence-derived families of glycoside hydrolases, polysaccharide lyases have been classified into just 12 families on the basis of amino acid sequence similarities ( 5 ), reflecting the requirement for substrate uronic acid groups in the elimination mechanism. (pnas.org)
Hyaluronate lyase2
Oligosaccharides4
- The invention is also directed to the use of the receptor for diagnostics of Helicobacter pylori, to a method of producing chondroitin oligosaccharides from chondroitin sulphates, and a method for production of amidated glucuronic acid comprising oligosaccharides and monosaccharides from glucuronic acid comprising polysaccharides. (sumobrain.com)
- Ulvan lyases depolymerise ulvan via the β-elimination mechanism with the release of oligosaccharides that have unsaturated 4-deoxy-L-threo-hex-4-enopyranosiduronic acid at the non-reducing end. (chalmers.se)
- Ulvan, which is one of the major structural polysaccharides of the cell walls of green macroalgae, is degraded by ulvan lyases via the β-elimination mechanism with the release of oligosaccharides that have unsaturated 4-deoxy-L-threo-hex-4-enopyranosiduronic acid (∆) at the non-reducing end. (chalmers.se)
- Thus, CS oligosaccharides from cartilaginous deer antler, with their oversulfated chondroitin sulfate composition, demonstrated the physiological properties and may be good candidates for osteogenetic agents in humans. (nih.gov)
Heparan sulfate and chondroitin sulfate1
- These hybrids contained heparan-sulfate and chondroitin-sulfate chains in similar molar amounts. (kuleuven.be)
Heparinum1
- Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum. (genome.jp)
Characterization2
- Purification and characterization of novel chondroitin ABC and AC. (ingentaconnect.com)
- Characterization of chondroitin sulfate from deer tip antler and osteogenic properties. (nih.gov)
Hyaluronan and chondroitin1
- Polysaccharide lyases (EC 4.2.2.x) are carbon-oxygen lyases that harness β-elimination chemistry (reviewed in ref. 1 ) to bring about degradation of C5 uronic acid containing pyranoside substrates such as polygalacturonates, alginates, hyaluronan, and chondroitin. (pnas.org)
Annotation1
- Following assembly and annotation, an orf, annotated as family 8 polysaccharide lyase genes, was identified, encoding an amino acid sequence with a similarity to CS lyases according to NCBI blastX. (springer.com)
Proteins1
- Among the predicted virulence factors, type IX secretion-mediated and cell-surface exposed proteins were identified including an atypical sialidase, a sphingomyelinase and a chondroitin AC lyase which activities were demonstrated in vitro . (frontiersin.org)
Disaccharide1
- Bakalash S, Rolls A, Lider O, Schwartz M (2007) Chondroitin sulfate-derived disaccharide protects retinal cells from elevated intraocular pressure in aged and immunocompromised rats. (springer.com)
Disaccharides1
- HS was digested into disaccharides by heparin lyases, and the compositions of 8 different disaccharides possessing sulfate residues at different combinations at the 2- O -, 6- O -, and N -positions were determined by HPLC analysis. (nature.com)
Chains6
- GL1 xanthan lyase, which acts on the side chains of xanthan. (ebi.ac.uk)
- Neuroglycan C lacking chondroitin sulfate chains was eluted with 0.5 m NaCl as a major fraction from the column. (biomedsearch.com)
- In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. (genome.jp)
- Chondroitin AC lyase and chondroitin ABC lyase digestions of these chains showed that they were composed of 75% dermatan sulphate and 25% chondroitin sulphate. (portlandpress.com)
- Purified brevican inhibits neurite outgrowth from cerebellar granule neurons in vitro , an activity that requires chondroitin sulfate chains. (jneurosci.org)
- The chondroitin sulfate chains, in contrast, showed no size heterogeneity. (kuleuven.be)
Ulvan lyases3
- Ulvan lyases belong to the PL24, PL25 or PL28 family in the CAZy database [2-5]. (chalmers.se)
- In contrast to the previously described ulvan lyases, this novel lyase is mostly composed of α-helices that form an (α/α)6 incomplete toroid domain and displays a remarkably broad-spectrum activity. (chalmers.se)
- This novel lyase is the first member of a new family, which sequence is not homologous to other ulvan lyases. (chalmers.se)
Proteus2
Chromatography2
- Plasma LDL-affinity chromatography of dermatan sulfate+chondroitin 4/6-sulfate fractions allowed the separation of LDL high- and low-affinity glycosaminoglycan species. (ahajournals.org)
- Chondroitin sulfate is the major glycosaminoglycan contained in antler connective tissue and has been isolated from cartilaginous antler by 4 M GuHCl extraction, gradient ultracentrifugation and chromatography techniques. (nih.gov)
Protein1
- Chondroitin ABC lyase digestion produced a core protein of Mr 45,000. (portlandpress.com)
Arthrobacter1
- Chondroitin Lyase from a Marine Arthrobacter sp. (springer.com)
Exolyase1
- Chondroitin-sulfate-ABC exolyase. (expasy.org)