Choline Kinase: An enzyme that is active in the first step of choline phosphoglyceride (lecithin) biosynthesis by catalyzing the phosphorylation of choline to phosphorylcholine in the presence of ATP. Ethanolamine and its methyl and ethyl derivatives can also act as acceptors. EC 2.7.1.32.Cytidine Diphosphate Choline: Donor of choline in biosynthesis of choline-containing phosphoglycerides.Phosphorylcholine: Calcium and magnesium salts used therapeutically in hepatobiliary dysfunction.Choline: A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.Hemicholinium 3: A potent inhibitor of the high affinity uptake system for CHOLINE. It has less effect on the low affinity uptake system. Since choline is one of the components of ACETYLCHOLINE, treatment with hemicholinium can deplete acetylcholine from cholinergic terminals. Hemicholinium 3 is commonly used as a research tool in animal and in vitro experiments.Phosphatidylcholines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.Choline O-Acetyltransferase: An enzyme that catalyzes the formation of acetylcholine from acetyl-CoA and choline. EC 2.3.1.6.MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Choline-Phosphate Cytidylyltransferase: An enzyme that catalyzes the transfer of cytidylate (CMP) to choline phosphate to form CDPcholine. It is the rate-limiting enzyme in the choline pathway for the biosynthesis of phosphatidylcholine. Its activity is increased by glucocorticoids. EC 2.7.7.15.Phosphotransferases: A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Choline Deficiency: A condition produced by a deficiency of CHOLINE in animals. Choline is known as a lipotropic agent because it has been shown to promote the transport of excess fat from the liver under certain conditions in laboratory animals. Combined deficiency of choline (included in the B vitamin complex) and all other methyl group donors causes liver cirrhosis in some animals. Unlike compounds normally considered as vitamins, choline does not serve as a cofactor in enzymatic reactions. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)ButanesEthanolamine: A viscous, hygroscopic amino alcohol with an ammoniacal odor. It is widely distributed in biological tissue and is a component of lecithin. It is used as a surfactant, fluorimetric reagent, and to remove CO2 and H2S from natural gas and other gases.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Protein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Calcium-Calmodulin-Dependent Protein Kinases: A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)Deanol: An antidepressive agent that has also been used in the treatment of movement disorders. The mechanism of action is not well understood.Thiocholine: A mercaptocholine used as a reagent for the determination of CHOLINESTERASES. It also serves as a highly selective nerve stain.src-Family Kinases: A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.Biochemical Phenomena: The chemical processes, enzymatic activities, and pathways of living things and related temporal, dimensional, qualitative, and quantitative concepts.Kinetics: The rate dynamics in chemical or physical systems.Protein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Cardiotoxins: Agents that have a damaging effect on the HEART. Such damage can occur from ALKYLATING AGENTS; FREE RADICALS; or metabolites from OXIDATIVE STRESS and in some cases is countered by CARDIOTONIC AGENTS. Induction of LONG QT SYNDROME or TORSADES DE POINTES has been the reason for viewing some drugs as cardiotoxins.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.p38 Mitogen-Activated Protein Kinases: A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mitogen-Activated Protein Kinase 1: A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.Phosphotransferases (Alcohol Group Acceptor): A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.Ethanolamines: AMINO ALCOHOLS containing the ETHANOLAMINE; (-NH2CH2CHOH) group and its derivatives.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.p21-Activated Kinases: A family of serine-threonine kinases that bind to and are activated by MONOMERIC GTP-BINDING PROTEINS such as RAC GTP-BINDING PROTEINS and CDC42 GTP-BINDING PROTEIN. They are intracellular signaling kinases that play a role the regulation of cytoskeletal organization.Betaine: A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)Mitogen-Activated Protein Kinase Kinases: A serine-threonine protein kinase family whose members are components in protein kinase cascades activated by diverse stimuli. These MAPK kinases phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES and are themselves phosphorylated by MAP KINASE KINASE KINASES. JNK kinases (also known as SAPK kinases) are a subfamily.JNK Mitogen-Activated Protein Kinases: A subgroup of mitogen-activated protein kinases that activate TRANSCRIPTION FACTOR AP-1 via the phosphorylation of C-JUN PROTEINS. They are components of intracellular signaling pathways that regulate CELL PROLIFERATION; APOPTOSIS; and CELL DIFFERENTIATION.Mitogen-Activated Protein Kinase 3: A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.Diacylglycerol Cholinephosphotransferase: An enzyme that catalyzes the synthesis of phosphatidylcholines from CDPcholine and 1,2-diacylglycerols. EC 2.7.8.2.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Serine: A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.Choline Dehydrogenase: An enzyme bound to the inner mitochondrial membrane that catalyzes the oxidation of CHOLINE to BETAINE.Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Creatine Kinase: A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.Methylcholanthrene: A carcinogen that is often used in experimental cancer studies.CDC2 Protein Kinase: Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.Cyclin-Dependent Kinases: Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events.MAP Kinase Kinase Kinases: Mitogen-activated protein kinase kinase kinases (MAPKKKs) are serine-threonine protein kinases that initiate protein kinase signaling cascades. They phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES; (MAPKKs) which in turn phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs).Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.eIF-2 Kinase: A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.Casein Kinase II: A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression.Casein Kinases: A group of protein-serine-threonine kinases that was originally identified as being responsible for the PHOSPHORYLATION of CASEINS. They are ubiquitous enzymes that have a preference for acidic proteins. Casein kinases play a role in SIGNAL TRANSDUCTION by phosphorylating a variety of regulatory cytoplasmic and regulatory nuclear proteins.Nucleotidyltransferases: A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Phosphorylation and regulation of choline kinase from Saccharomyces cerevisiae by protein kinase A. (1/136)

The CKI1-encoded choline kinase (ATP:choline phosphotransferase, EC 2.7.1.32) from Saccharomyces cerevisiae was phosphorylated in vivo on multiple serine residues. Activation of protein kinase A activity in vivo resulted in a transient increase in the phosphorylation of choline kinase. This phosphorylation was accompanied by a stimulation in choline kinase activity. In vitro, protein kinase A phosphorylated choline kinase on a serine residue with a stoichiometry (0.44 mol of phosphate/mol of choline kinase) consistent with one phosphorylation site/choline kinase subunit. The major phosphopeptide derived from the enzyme phosphorylated in vitro by protein kinase A was common to one of the major phosphopeptides derived from the enzyme phosphorylated in vivo. Protein kinase A activity was dose- and time-dependent and dependent on the concentrations of ATP (Km 2.1 microM) and choline kinase (Km 0.12 microM). Phosphorylation of choline kinase with protein kinase A resulted in a stimulation (1.9-fold) in choline kinase activity whereas alkaline phosphatase treatment of choline kinase resulted in a 60% decrease in choline kinase activity. The mechanism of the protein kinase A-mediated stimulation in choline kinase activity involved an increase in the apparent Vmax values with respect to ATP (2.6-fold) and choline (2.7-fold). Overall, the results reported here were consistent with the conclusion that choline kinase was regulated by protein kinase A phosphorylation.  (+info)

In vivo antitumor activity of choline kinase inhibitors: a novel target for anticancer drug discovery. (2/136)

Transformation by some oncogenes is associated with increased activity of choline kinase (ChoK), resulting in elevated constitutive levels of phosphorylcholine, a proposed second messenger required for DNA synthesis induced by growth factors. Here we describe the characterization of ChoK inhibitors with antiproliferative properties against human tumor-derived cell lines. The new molecules were tolerated in mice at doses that showed in vivo antitumor activity against human tumor xenografts derived from HT-29 and A431 cell lines implanted s.c. in nude mice. This first generation of inhibitors provides in vivo evidence that blockade of phosphorylcholine production is a valid strategy for the development of new anticancer agents, opening a new avenue for the development of antitumor drugs with a novel mechanism of action.  (+info)

Extracellular calcium stimulates DNA synthesis in synergism with zinc, insulin and insulin-like growth factor I in fibroblasts. (3/136)

In serum-starved mouse NIH 3T3 fibroblasts cultured in 1.8 mM Ca2+-containing medium, addition of 0.75-2 mM extra Ca2+ stimulated DNA synthesis in synergism with zinc (15-60 microM), insulin and insulin-like growth factor I. Extra Ca2+ stimulated phosphorylation/activation of p42/p44 mitogen-activated protein kinases by an initially (10 min) zinc-independent mechanism; however, insulin, and particularly zinc, significantly prolonged Ca2+-induced mitogen-activated protein kinase phosphorylation. In addition, extra Ca2+ activated p70 S6 kinase by a zinc-dependent mechanism and enhanced the stimulatory effect of zinc on choline kinase activity. Insulin and insulin-like growth factor I also commonly increased both p70 S6 kinase and choline kinase activities. In support of the role of the choline kinase product phosphocholine in the mediation of mitogenic Ca2+ effects, cotreatments with the choline kinase substrate choline (250 microM) and the choline kinase inhibitor hemicholinium-3 (2 mM) enhanced and inhibited, respectively, the combined stimulatory effect of extra Ca2+ (3.8 mM total) and zinc on DNA synthesis. In various human skin fibroblast lines, 1-2 mM extra Ca2+ also stimulated DNA synthesis in synergism with zinc and insulin. The results show that in various fibroblast cultures, high concentrations of extracellular Ca2+ can collaborate with zinc and certain growth factors to stimulate DNA synthesis. Considering the high concentration of extracellular Ca2+ in the dermal layer, Ca2+ may promote fibroblast growth during wound healing in concert with zinc, insulin growth factor-I insulin, and perhaps other growth factors.  (+info)

Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta. (4/136)

Choline/ethanolamine kinase (CK/EK) is the first enzyme in phosphatidylcholine/phosphatidylethanolamine biosynthesis in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products so far were all shown to have EK activity also, indicating that both activities reside on the same protein. CK/EK in most animal cells exists as several isoforms, for two of which (alpha and beta) their cDNAs have been cloned from both the rat and mouse, and they are found to be separate gene products. The physiological significance for the existence of more than one CK/EK enzyme, however, remains to be clarified. In this study, we isolated mouse genes encoding both types of CK/EK isozyme and determined their entire structure. The 5'-flanking promoter regions were found to have quite different features from each other, indicating that their expression could be under distinct control. Comparison of the nucleotide sequence between the corresponding coding exons showed the best homology (75%) residing on exon VIII. A search of the database resulted in the possible existence of 17 different origins of eukaryotic CK and/or EK, each of which presumably contained the entire amino acid sequence. Multialignment of their putative amino acid sequences led to an identification of the novel consensus sequence possibly required for the expression of either CK or EK activity, which corresponded to the sequence within exons VII and VIII of CK/EK-alpha and -beta genes from the mouse. This sequence was localized in close proximity to the C-terminal region of the general (Brenner's) phosphotransferase concensus sequence which was also completely conserved in all of the putative eukaryotic CK/EK proteins. The results demonstrated that, while both CK/EK-alpha and -beta genes were composed of 11 major exons, the size of their genes was quite different: 40 kb for CK/EK-alpha, whereas it was only 3.5 kb for CK/EK-beta.  (+info)

Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I. (5/136)

For characterization of the detailed gene structure of human muscle type carnitine palmitoyltransferase I (M-CPTI), we analyzed the 5'-upstream region of the M-CPTI transcripts. As a result, we found a cDNA clone containing a nucleotide sequence unexpected from the reported M-CPTI gene structure in the upstream region of its 5' end. Comparison of this nucleotide sequence with that of genomic DNA showed that this sequence was derived from the 3'-untranslated region of the gene encoding choline/ethanolamine kinase-beta (CK/EK-beta) located upstream of the M-CPTI gene. Southern blot analysis showed that there was no other region homologous to the CK/EK-beta gene in the whole human genome. Thus, the overlapping transcript was concluded to be produced from the functional genes of CK/EK-beta and M-CPTI. Furthermore, cDNAs containing both exons of these genes were detected by the polymerase chain reaction using the cDNA of human heart M-CPTI obtained by specific reverse transcription from its 3'-untranslated region as a template. From these results, the production and organization of these overlapping transcripts are discussed.  (+info)

Pharmacological inhibition of phosphatidylcholine biosynthesis is associated with induction of phosphatidylinositol accumulation and cytolysis of neoplastic cell lines. (6/136)

De novo production of phosphatidic acid (PA) in tumor cells is required for phospholipid biosynthesis and growth of tumor cells. In addition, PA production by phospholipase D has been cited among the effects of certain oncogenes and growth factors. In this report, it has been demonstrated that enhanced phospholipid metabolism through PA in tumor cells can be exploited pharmacologically for development of anticancer agents, such as CT-2584, a cancer chemotherapeutic drug candidate currently in Phase II clinical trials. By inhibiting CTP:choline-phosphate cytidylyltransferase (CT), CT-2584 caused de novo phospholipid biosynthesis via PA to be shunted away from phosphatidylcholine (PC) and into phosphatidylinositol (PI), the latter of which was doubled in a variety of CT-2584-treated tumor cell lines. In contrast, cytotoxic concentrations of cisplatin did not induce accumulation of PI, indicating that PI elevation by CT-2584 was not a general consequence of chemotherapy-induced cell death. Consistent with this mechanism of action, propranolol, an inhibitor of PA phosphohydrolase and phosphatidylcholine biosynthesis, was also cytotoxic to tumor cell lines, induced PI accumulation, and potentiated the activity of CT-2584 in cytotoxicity assays. As expected from biophysical properties of anionic phospholipids on cellular membranes, CT-2584 cytotoxicity was associated with disruption and swelling of endoplasmic reticulum and mitochondria. We conclude that CT-2584 effects a novel mechanism of cytotoxicity to cancer cells, involving a specific modulation of phospholipid metabolism.  (+info)

Modulation of phospholipase D by hexadecylphosphorylcholine: a putative novel mechanism for its antitumoral activity. (7/136)

Hexadecylphosphorylcholine (HePC, D-18506, INN: Mitelfosine) belongs to the family of alkylphosphocholines with anticancer activity. Previous reports have related its antitumoral activity to their ability to interfere with phospholipid metabolism. However a clear mechanism of action has not been established yet. We have investigated the effect of HePC on two enzymes recently reported to play a role in cell growth proliferation, phospholipase D (PLD) and choline kinase (ChoK). Our results demonstrate that treatment with HePC induces a rapid stimulation of PLD, that may be achieved by PKC dependent or independent mechanisms, depending on the cell line investigated. Both PLD1 and PLD2 isoenzymes are sensitive to HePC activation. By contrast, no effect was observed by HePC on ChoK, a new target for anticancer drug development. Furthermore, in all cell lines tested, a chronic exposure of the cells to HePC abrogates PLD activation by either phorbol esters or HePC itself with no effect on total cellular PLD levels. This is reflected in a strong inhibition of PLD activity. We suggest that the inhibitory effects on PLD by HePC may be related to its antitumoral action.  (+info)

Regulation of choline kinase activity by Ras proteins involves Ral-GDS and PI3K. (8/136)

Ras proteins are molecular switches that control signaling pathways critical in the onset of a variety of human cancers. The signaling pathways activated by Ras proteins are those controlled by its direct effectors such as the serine-threonine protein kinase Raf-1, the exchange factor for other GTPases Ral-GDS, and the lipid kinase PI3K. As a consequence of Ras activation, a number of additional enzymes are affected, including several members of the serine-threonine intracellular proteins kinases as well as enzymes related to phospholipid metabolism regulation such as phospholipases A2 and D, and choline kinase. The precise mechanisms by which ras oncogenes impinge into these later molecules and their relevance to the onset of the carcinogenic process is still not fully understood. Here we have investigated the mechanism of regulation of choline kinase by Ras proteins and found no direct link between PLD and choline kinase activation. We provide evidence that Ras proteins regulate the activity of choline kinase through its direct effectors Ral-GDS and PI3K, while the Raf pathways seems to be not relevant in this process. The importance of Ras-dependent activation of choline kinase is discussed.  (+info)

*Choline kinase

... (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first reaction in the choline ... which showed for the first time that choline kinase α can act as a protein chaperone (Asim et al., 2016 ;JNCI "Choline kinase ... Peisach, D; Gee, P; Kent, C; Xu, Z (2003). "The Crystal Structure of Choline Kinase Reveals a Eukaryotic Protein Kinase Fold". ... A related enzyme, ethanolamine kinase, tends to co-purify with choline kinase leading to a suggestion that the two activities ...

*Choline/ethanolamine kinase family

In molecular biology, the choline/ethanolamine kinase family includes choline kinase(EC 2.7.1.32) and ethanolamine kinase (EC ... Ethanolamine kinase has no choline kinase activity and its activity is inhibited by ADP. Inositol supplementation represses ... Kim KH, Voelker DR, Flocco MT, Carman GM (March 1998). "Expression, purification, and characterization of choline kinase, ... There are two putative choline/ethanolamine kinases (C/EKs) in the Trypanosoma brucei genome. ...

*CHKA

Spanner S, Ansell GB (1979). "Choline kinase and ethanolamine kinase activity in the cytosol of nerve endings from rat ... Choline kinase alpha is an enzyme that in humans is encoded by the CHKA gene. The major pathway for the biosynthesis of ... 2002). "Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate, and ... "Entrez Gene: CHKA choline kinase alpha". Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using ...

*CHKB (gene)

Choline kinase beta is a protein encoded by the CHKB gene. This gene is found on chromosome 22 in humans. Choline kinase (CK) ... The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function ... "Entrez Gene: CHKB choline kinase beta". Human CHKB genome location and CHKB gene details page in the UCSC Genome Browser. Human ... Ishidate K (1997). "Choline/ethanolamine kinase from mammalian tissues". Biochim. Biophys. Acta. 1348 (1-2): 70-8. doi:10.1016/ ...

*RALGDS

Ramírez de Molina A, Penalva V, Lucas L, Lacal JC (2002). "Regulation of choline kinase activity by Ras proteins involves Ral- ... a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase ... Li W, Han M, Guan KL (April 2000). "The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex ... "Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras". Cell. 89 (3): 457-67 ...

*Miltefosine

2007). "Inhibition of Plasmodium falciparum choline kinase by hexadecyltrimethylammonium bromide: a possible antimalarial ...

*Phosphocholine

... is made in a reaction, catalyzed by choline kinase, that converts ATP and choline into phosphocholine and ADP. ... Alkylphosphocholines Choline Phosphoethanolamine Lovell TM, Woods RJ, Butlin DJ, et al. (September 2007). "Identification of a ...

*CHKB

... may refer to: Choline kinase beta, a human gene Christian Historical Voters' League, a Dutch conservative protestant ...

*List of EC numbers (EC 2)

... glycerone kinase EC 2.7.1.30: glycerol kinase EC 2.7.1.31: glycerate kinase EC 2.7.1.32: choline kinase EC 2.7.1.33: ... subunit kinase EC 2.7.11.24: mitogen-activated protein kinase EC 2.7.11.25: mitogen-activated protein kinase kinase kinase EC ... EC 2.7.1.71: shikimate kinase EC 2.7.1.72: streptomycin 6-kinase EC 2.7.1.73: inosine kinase EC 2.7.1.74: deoxycytidine kinase ... NAD+ kinase EC 2.7.1.24: dephospho-CoA kinase EC 2.7.1.25: adenylyl-sulfate kinase EC 2.7.1.26: riboflavin kinase EC 2.7.1.27: ...

*List of MeSH codes (D08)

... adenosine kinase MeSH D08.811.913.696.620.155 --- choline kinase MeSH D08.811.913.696.620.175 --- deoxycytidine kinase MeSH ... map kinase kinase kinases MeSH D08.811.913.696.620.682.700.559.100 --- map kinase kinase kinase 1 MeSH D08.811.913.696.620.682. ... map kinase kinase kinase 2 MeSH D08.811.913.696.620.682.700.559.300 --- map kinase kinase kinase 3 MeSH D08.811.913.696.620.682 ... map kinase kinase kinase 4 MeSH D08.811.913.696.620.682.700.559.500 --- map kinase kinase kinase 5 MeSH D08.811.913.696.620.682 ...

*CDP-choline pathway

Mst other cells will phosphorylate choline by the enzyme choline kinase, the first committed step of CDP-choline pathway. ... Under normal circumstances, choline kinase is not the rate-limiting step of the CDP-choline pathway. However in rapidly ... Choline kinase (CK) is a cytosolic protein that catalyzes the following reaction: choline + ATP ⇌ phosphocholine + ADP In ... The first enzymatic reaction is catalyzed by choline kinase (CK) and involves the phosphorylation of choline to form ...

*Amifampridine

... particularly those with defects in choline acetyltransferase, downstream kinase 7, and those where any kind of defect causes " ...

*Ethanolamine kinase

Sung CP, Johnstone RM (1967). "Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells". Biochem. J. 105 ... In enzymology, an ethanolamine kinase (EC 2.7.1.82) is an enzyme that catalyzes the chemical reaction ATP + ethanolamine ⇌ {\ ... Other names in common use include ethanolamine kinase (phosphorylating), and ethanolamine phosphokinase. This enzyme ...

*3q29 microdeletion syndrome

These include transferrin receptor, choline-phosphate cytidylyltransferase A, RNF168, serine/threonine-protein kinase, nuclear ...

*CDKN3

It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase ... Niculescu MD; Yamamuro Y; Zeisel SH (2004). "Choline availability modulates human neuroblastoma cell proliferation and alters ... Cyclin-dependent kinase inhibitor 3 is an enzyme that in humans is encoded by the CDKN3 gene. The protein encoded by this gene ... 2000). "Overexpression of kinase-associated phosphatase (KAP) in breast and prostate cancer and inhibition of the transformed ...

*Phospholipase D

TYK and controls the signalling indicating that PLD is activated by these kinases. As choline is very abundant in the cell, PLD ... Phosphatidyl choline Phosphatidate Choline Phospholipase cleavage sites Jenkins GM, Frohman MA (October 2005). "Phospholipase D ... As choline has little second messenger activity, PLD activity is mostly transduced by the production of PA. PA is heavily ... Paruch S, El-Benna J, Djerdjouri B, Marullo S, Périanin A (January 2006). "A role of p44/42 mitogen-activated protein kinases ...

*PLD2

Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled ... catalyze the hydrolysis of PC to produce phosphatidic acid and choline. ... "Phosphorylation-dependent regulation of phospholipase D2 by protein kinase C delta in rat Pheochromocytoma PC12 cells". J. Biol ... "Phosphorylation-dependent regulation of phospholipase D2 by protein kinase C delta in rat Pheochromocytoma PC12 cells". J. Biol ...

*Phospholipase D1

A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC- ... EC 3.1.4.4 catalyze the hydrolysis of PC to produce phosphatidic acid and choline. ... Protein kinase N1 RALA, and RHOA. VU-0359595: 1,700-fold selective versus phospholipase D2, IC50 = 3.7nM. GRCh38: Ensembl ... regulation by protein kinase Calpha and caveolin-1". Biochemistry. 38 (12): 3763-9. doi:10.1021/bi982478+. PMID 10090765. Kim Y ...

*Phosphatidic acid

... to produce PA and choline. By the phosphorylation of diacylglycerol (DAG) by DAG kinase (DAGK) By the acylation of ... Because choline is very abundant in the cell, PLD activity does not significantly affect choline levels; and choline is ... October 2004). "Sphingosine kinase 1 is an intracellular effector of phosphatidic acid". J. Biol. Chem. 279 (43): 44763-74. doi ... This will show whether the phosphate group is newly derived from the kinase activity or whether it originates from the PC. ...

*X chromosome

... encoding enzyme Choline-phosphate cytidylyltransferase B PIN4: encoding enzyme Peptidyl-prolyl cis-trans isomerase NIMA- ... encoding protein Ribosomal protein S6 kinase, 90kDa, polypeptide 6 RRAGB: encoding protein Ras-related GTP-binding protein B ... encoding enzyme Nik-related protein kinase OTUD5: encoding protein OTU deubiquitinase 5 PASD1: encoding protein PAS domain- ...

*Cholinergic neuron

The problem with this therapy is that choline acetyltransferase is largely blocked by the blood-brain barrier. PTD-ChAT is a ... It should also be noted that memantine is able to inhibit the truncation of glycogen synthase kinase-3 (triggered by activated ... It is normal in aging for circadian rhythms to deteriorate as choline acetyltransferase (ChAT) fluctuations change in pattern ... Alzheimer's typically involves a decline in the activity of choline acetyltransferase and acetylcholinesterase, as well as a ...

*List of biomolecules

K252a K252b KT5720 KT5823 Keratin Kinase For substances with an l- or L- prefix such as L-alanine or DL-alanine, please see the ... Cholesterol Choline Chondroitin sulfate Cinnamaldehyde Citral Citric acid Citrinin Citronellal Citronellol Citrulline Cobalamin ... Cortisol Creatine Creatine kinase Crystallin α-Cyclodextrin Cyclodextrin glycosyltransferase Cyclopamine Cyclopiazonic acid ... superoxide T2 Toxin Tannic acid Tannin Tartaric acid Taurine Tetrodotoxin Thaumatin Topoisomerase Tyrosine kinase Testosterone ...

*Phosphatidylethanolamine N-methyltransferase

While the CDP-choline pathway, in which choline obtained either by dietary consumption or by metabolism of choline-containing ... phosphorylated AMP-activated protein kinase (AMPK), and serine-307-phosphorylated insulin receptor substrate 1 (IRS1-s307), ... PC made via the PEMT pathway can be degraded by phospholipases C/D, resulting in the de novo formation of choline. Thus, the ... This is one of several examples of the reciprocal regulation of PEMT and CT in the PEMT and CDP-choline pathways. Estrogen has ...

*PAWR

Xie J, Guo Q (Jul 2004). "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma ... Kawai T, Akira S, Reed JC (Sep 2003). "ZIP kinase triggers apoptosis from nuclear PML oncogenic domains". Molecular and ... PAWR has been shown to interact with: Apoptosis antagonizing transcription factor, DAPK3, Protein kinase Mζ, SLC5A1, THAP1, and ... interacts selectively with the atypical isoforms of protein kinase C". Cell. 86 (5): 777-86. doi:10.1016/s0092-8674(00)80152-x ...

*SLC44A1

"Impaired trafficking of choline transporter-like protein-1 at plasma membrane and inhibition of choline transport in THP-1 ... phosphatase 2calpha-Ca2+ channel complex for dephosphorylation of neuronal Ca2+ channels phosphorylated by protein kinase C". ... "Differential expression and regulation of the high-affinity choline transporter CHT1 and choline acetyltransferase in neurons ... Choline transporter-like protein 1 is a protein that in humans is encoded by the SLC44A1 gene. Cluster of differentiation ...

*Enzyme inhibitor

Protein kinases can also be inhibited by competition at the binding sites where the kinases interact with their substrate ... acetate and choline. This is somewhat unusual among neurotransmitters as most, including serotonin, dopamine, and ... As a consequence, if two protein kinase inhibitors both bind in the active site with similar affinity, but only one has to ... For example, some protein kinase inhibitors have chemical structures that are similar to adenosine triphosphate, one of the ...
Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009 ...
Ethanolamine and choline are major components of the trypanosome membrane phospholipids, in the form of GPEtn (glycerophosphoethanolamine) and GPCho (glycerophosphocholine). Ethanolamine is also found as an integral component of the GPI (glycosylphosphatidylinositol) anchor that is required for membrane attachment of cell-surface proteins, most notably the variant-surface glycoproteins. The de novo synthesis of GPEtn and GPCho starts with the generation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinases via the Kennedy pathway. Database mining revealed two putative C/EKs (choline/ethanolamine kinases) in the Trypanosoma brucei genome, which were cloned, overexpressed, purified and characterized. TbEK1 (T. brucei ethanolamine kinase 1) was shown to be catalytically active as an ethanolamine-specific kinase, i.e. it had no choline kinase activity. The Km values for ethanolamine and ATP were found to be 18.4±0.9 and 219±29 μM respectively. TbC/EK2 (T. brucei choline
CHKA - CHKA (untagged)-Human choline kinase alpha (CHKA), transcript variant 1 available for purchase from OriGene - Your Gene Company.
sp:CHKB_MOUSE] Chkb, Chetk, Chkl, Ck, Ck/Ek, Ck/Ek-beta, Ckb, Ckekb, Ek; choline kinase beta; K14156 choline/ethanolamine kinase [EC:2.7.1.32 2.7.1.82] ...
Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first reaction in the choline pathway for phosphatidylcholine (PC) biosynthesis. This reaction involves the transfer of a phosphate group from adenosine triphosphate (ATP) to choline in order to form phosphocholine. ATP + choline ⇌ {\displaystyle \rightleftharpoons } ADP + O-phosphocholine Thus, the two substrates of this enzyme are ATP and choline, whereas its two products are adenosine diphosphate (ADP) and O-phosphocholine. Choline kinase requires magnesium ions (+2) as a cofactor for this reaction. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The first detailed investigation of the enzyme was conducted by McCamen in 1962, where it was shown that the brain is the richest source of the enzyme in mammalian tissue. A related enzyme, ethanolamine kinase, tends to co-purify ...
Order monoclonal and polyclonal Choline Kinase alpha antibodies for many applications. Selected quality suppliers for anti-Choline Kinase alpha antibodies.
Between generations, descendants of lineages exposed to successive generations of recurring drought exhibited only four transgenerational drought stress-associated DMRs compared with unstressed lineages. Significantly, none of these were in common with the 40 drought-associated epi-alleles detected within a generation, reinforcing the notion that transgenerational adaptive DNA methylation is a rare occurrence, even under conditions of abiotic stress (Pecinka et al., 2009; Seymour et al., 2014; Eichten and Springer, 2015; Hagmann et al., 2015; Secco et al., 2015; Crisp et al., 2016). Three of the four mapped to repetitive and already heavily methylated regions of the genome. The fourth DMR also was in intergenic space, albeit 800 bp upstream from CEK3. CEK3 encodes a protein, most highly transcribed in the hypocotyl, that is a part of the Choline/Ethanolamine Kinase family, for which CEK4 has been implicated in phospholipid biosynthesis and embryo development; however, mutation of CEK3 did not ...
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
Kit Component:- KN210253G1, CHKB gRNA vector 1 in pCas-Guide vector- KN210253G2, CHKB gRNA vector 2 in pCas-Guide vector- KN210253D, donor vector…
We have shown previously that N-[2-bromocinnamyl(amino)-ethyl]-5-isoquinolinesulphonamide (H-89), a selective inhibitor of cyclic-AMP-dependent protein kinase (PKA), inhibits phosphatidylcholine biosynthesis in HeLa cells. In the present study, we elucidated the mechanism underlying the described inhibition. Treatment of cells with 10 microM H-89 had no effect on the phosphorylation of CTP:phosphocholine cytidylyltransferase. However, H-89 slightly affected the distribution of cytidylyltransferase between cytosol and membranes, but the cellular 1,2-diacylglycerol content was not influenced. Furthermore, pulse-chase experiments revealed that H-89 did not affect cytidylyltransferase activity. Instead, H-89 inhibited choline kinase, the enzyme catalysing the first step in the CDP-choline pathway. In the presence of 10 microM H-89, choline kinase activity was inhibited by 36 +/- 7.6% in vitro. Additionally, the phosphorylation of choline to phosphocholine was inhibited by 30 +/- 3% in cell-culture ...
Choline Kinase: An enzyme that is active in the first step of choline phosphoglyceride (lecithin) biosynthesis by catalyzing the phosphorylation of choline to phosphorylcholine in the presence of ATP. Ethanolamine and its methyl and ethyl derivatives can also act as acceptors. EC 2.7.1.32.
Complete information for CHKB gene (Protein Coding), Choline Kinase Beta, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for CHKB gene (Protein Coding), Choline Kinase Beta, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Choline is an essential nutrient that has similarities to the B vitamins. Although the human body makes some choline, choline needs to be obtained through the diet…
Learn more about Choline uses, effectiveness, possible side effects, interactions, dosage, user ratings and products that contain Choline
Choline Hydrogentartrate is a medicine available in a number of countries worldwide. A list of US medications equivalent to Choline Hydrogentartrate is available on the Drugs.com website.
Results The validity of CHKA-antibody was verified using CHKA-transfected cells and siRNA knockdown. Immunoblotting of tissues showed good resolution of CHKA protein in malignant prostate, verifying use of the antibody for IHC. There was minimal qRT-PCR detectable CHKA mRNA in normal tissue, and conversely high expression in malignant prostate tissues. IHC of normal prostate cores showed mild (intensity) CHKA expression in only 28% (7/25) of samples with no Ki67 expression. In contrast, CHKA was expressed in all malignant prostate cores along with characteristically low proliferation (median 2% Ki67-LI; range 1-17%). Stratification of survival according to CHK intensity showed a trend towards lower progression-free survival with CHK score of 3. ...
Gentaur molecular products has all kinds of products like :search , AbD \ GOAT ANTI HUMAN CHOLINE ACETYLTRANSFERASE, Product Type Polyclonal Antibody, Specificity CHOLINE ACETYLTRANSFERASE, Target Species Human, Host Goat, Format Serum, Isotypes Polyclonal IgG, Applic \ 2080-0000 for more molecular products just contact us
Glycerophospholipid metabolism. Left, glycerophospholipid synthesis; Right, glycerophospholipid degradation. The enzymes are choline kinase (ChoK), ethanolamine
Catalytic domain of the Serine/Threonine Kinase, PCTAIRE-1 kinase. Serine/Threonine Kinases (STKs), PCTAIRE-1 subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PCTAIRE-1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. PCTAIRE-1 shares sequence similarity with Cyclin-Dependent Kinases (CDKs), which belong to a large family of STKs that are regulated by their cognate cyclins. Together, CDKs and cyclins are involved in the control of cell-cycle progression, transcription, and neuronal function. PCTAIRE-1 is expressed ubiquitously and is localized in the cytoplasm. Its kinase activity is cell cycle dependent and peaks at the S and G2 phases. PCTAIRE-1 is highly expressed in the brain and may play a role in regulating neurite ...
Catalytic domain of the Serine/Threonine Kinase, Cyclin-Dependent protein Kinase 5. Serine/Threonine Kinases (STKs), Cyclin-Dependent protein Kinase 5 (CDK5) subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The CDK5 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. CDKs belong to a large family of STKs that are regulated by their cognate cyclins. Together, they are involved in the control of cell-cycle progression, transcription, and neuronal function. CDK5 is unusual in that it is regulated by non-cyclin proteins, p35 and p39. It is highly expressed in the nervous system and is critical in normal neural development and function. It plays a role in neuronal migration and differentiation, and is also important in synaptic ...
When it comes to adding choline to your diet, there is not a set way that is "right". You must figure out what is best for your needs and desires, but it is a good idea to ensure you have enough at all times. You are right to consider choline an important part of your diet. It is important that you make sure you are getting enough because it is the precursor for a specific brain chemical called acetylcholine, which helps for many aspects of brain health.. If you are just getting started with brain enhancement, you need to know that nutrition is the first key for getting a more improved cognitive ability. Having the right nutrients, such as vitamins and minerals, can be a bigger boost for your brain than some of the latest and greatest drugs. That is why you need to get more choline into your diet. One way to do that is through eggs, which is the simplest and probably most delicious way that youll be able to get all of the choline that you need. If you dont feel comfortable eating that many ...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
CHOLINE is required for the proper transmission of nerve impulses from the brain through the central nervous system. It is also needed for gall bladder regulation, liver function, lecithin formation, and cardiovascular health. It aids in hormone production and minimizes excess fat in the liver through fat and cholesterol metabolism. Without choline, brain function and…
Choline is a vital component of all cell membranes. Choline is part of the B-Complex vitamins and is critical for the optimal functioning of cells.FREE OF:
Choline methyl sulfate/ACM65151624 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
DEPRESSION IS A POSSIBLE SIDE-EFFECT OF ORAL CHOLINE. Inpharma Wkly. 60, 5 (1976). https://doi.org/10.1007/BF03288976. Download ...
Milhouse @ 163. "Keep the Sabbath day to sanctify it" (Deuteronomy 5:11). This actually doesnt mean "keep" in the sense of observing, like we say "This person keeps Shabbos". If you think about it, you have to agree.. It means be careful of the day, or arrange your activities so that it is possible. It is the type of thing that you have to prepare, before you do it. I could say also remember, but thats Zachor. This is a little more than just not losing track of the day.. http://mechon-mamre.org/p/pt/pt0326.htm. Here we see Lev 29:3 If ye walk in My statutes, and keep My commandments, and do them;. Exceot wait - it doesnt mean keep. This is translated all wrong. First, it is not statutes, but more like protocols or regulations - something assigned. A "chok" is supposed to mean, acording to one explanation, things that have no logical explanation, but it really means something (semi) arbitrary, not essential morality. Maybe it is something where you have to have something but it could be this ...
Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.. ...
After years of using mostly cloth sheet masks, Ive come to the realization that Ive been using the wrong ones all along. Well, maybe not the WRONG ones (I think its hard to go wrong with a sheet mask for me), but being a super dry skin type, the hydrogel masks feel so much better on…
HOLY FUCKS, THIS MASK. A dear friend sent a k-beauty care package my way recently and included this mask in the box. I finally decided to give it a shot last night. Id like to apologize for not having any pics of the actual mask as I was not expecting this to be anything other…
... is a vitamin supplement that is present in several forms, including phosphatidylcholine (PC). The medicinal effects of choline are presumed to be caused by phosphatidylcholine. PC is a major part of the membranes surrounding our cells. When PC is eaten, it is broken down into choline rather than being carried directly to the cell membranes. Choline acts like another commonly used supplement, SAMe (S-adenosylmethionine), to provide the methyl groups used by the body in a number of chemical reactions, including the detoxification pathways of the liver. It is also used to make acetylcholine and dopamine, nerve chemicals essential for normal brain function. Choline may also aid in brain and liver function by assisting with the absorption of carnitine from the digestive tract. Carnitine is essential for mitochondrial function, ensuring the liver and brain have an adequate supply of energy.. ...
Partial charge distribution on first and second neighbor atoms to nitrogen in choline and ethanolamine have been calculated. Coulombic and steric parameters were then utilized to evaluate the interaction of a negative test charge with the two molecules. Both the position and the magnitude of the maximum of interaction energy in the two systems were significantly different. The results suggest that ethanolamine interacts more strongly with anions than choline does. This is due principally to steric repulsion of the negative charge by the methyl groups in choline. ...
MZ-STORE Choline & Inositol 120 caps. • Dietary Supplements MZ-Store.co.uk • Choline Choline & Inositol - 39 ••• CHECK IT!
Choline containing medications, Choline indications and usages ATC and ICD codes, combinations with other active ingredients and trade names information from Drugs-about.com
Choline containing medications, Choline indications and usages ATC and ICD codes, combinations with other active ingredients and trade names information from Drugs-about.com
choline definition: an all-natural amine, C5H15NO2, frequently classed into the vitamin B complex and a constituent of many various other biologically important molecules, such acetylcholine and lecithin.;…
Reducing cholesterol is one of the primary uses for choline fenofibrate. This eMedTV segment features more details on what this fibrate drug is used for and also includes information on how it works and who can take it.
Irelands premier place to shop for Solaray Choline. eVitamins is a trusted and approved merchant to sell authentic Solaray items. See reviews, find coupons and more. Even get fast, reliable shipping to Ireland
Thermophysical properties and gas solubilities in choline chloride-urea for CO2 separation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Learn more about Choline at Grand Strand Medical Center Uses Principal Proposed Uses Alzheimers Disease ...
In prostate cancer, citrate may be a marker of responsiveness to treatment based on a pilot study where 16 high-risk prostate cancer patients were treated with chemotherapy, hormones, radical prostatectomy, or radiotherapy, and subsequently followed with prostate-specific antigen monitoring, MRI, and MRSI (53, 54). A risk score using MRSI was developed based on both tumor volume and metabolic abnormality. The MRSI score and MRI tumor/node stage was then used to determine prostate-specific antigen relapse and was predictive in 15 of 16 cases.. There is an ongoing effort by the National Cancer Institute, researchers, clinicians, and industry to expand the use of metabolomics, with particular attention to MRSI for the assessment of therapeutic response (55). Choline phospholipid metabolism intermediates have been deemed a potential biomarker for monitoring treatment efficacy in a variety of human cancers (reviewed in ref. 12). In general, a decrease in the tCho signal on 1H-NMR equates to a ...
Définitions de Hemicholinium-3, synonymes, antonymes, dérivés de Hemicholinium-3, dictionnaire analogique de Hemicholinium-3 (anglais)
Save 38% Lindberg - Choline & Inositol 500 mg 100 Capsules Choline & Inositol 500 mg Supports Brain and Nervous System Health* Choline & Inositol are B-vitamin-like substances that are essential components of all cell membranes.* Choline supports brain health and is a component of acetylcholine, a neurotransmitter in the brain.* Choline also supports fat metabolism in the liver.* Inositol is required for the proper function of the brain and nerves.* Inositol is a fundamental ingredient of cell membranes and plays a role in its structure and integrity.*
Choline Info! In addition to fatty liver, choline deficiency causes abnormal kidney function, infertility, growth impairment, bone abnormalities, reduced protection of blood cells and hypertension, among other problems. Use of choline as a dietary supplement can lead to improved cardiovascular health, liver and reproductive functions. Choline has been shown to improve performance in endurance sports such as running and swimming. Choline is known to play a role in brain development, learning and memory.. ...
Looking for choline esterase II? Find out information about choline esterase II. An enzyme found in blood and in various other tissues that catalyzes hydrolysis of choline esters, including acetylcholine. Abbreviated chE. any of a group... Explanation of choline esterase II
Looking for choline esterase I? Find out information about choline esterase I. An enzyme found in excitable membranes that inactivates acetylcholine Explanation of choline esterase I
Choline salicylate + cetalkonium chloride is used in the treatment of .get complete information about choline salicylate + cetalkonium chloride including usage, side effects, drug interaction, expert advice along with medicines associated with choline salicylate + cetalkonium chloride at 1mg.com
View images of Choline magnesium trisalicylate and identify pills by imprint code, shape and color with the Drugs.com Pill Identifier.
Background: Choline status has been associated with stunting among young children. Findings from this study showed that an egg intervention improved linear growth by a length-for-age z score of 0.63.Objective: We aimed to test the efficacy of eggs introduced early in complementary feeding on plasma concentrations of biomarkers in choline pathways, vitamins B-12 and A, and
Non-profit foundation providing reliable, scientifically accurate, personalized information for convenient and enjoyable healthy eating.
A new study finds an association between two genes involved in choline metabolism and the risk of spina bifida. The study, published in the open access journal BMC Medicine, also shows that this association is independent of dietary choline intake by the mother during pregnancy.. Choline is a nutrient, essential for cardiovascular and brain function, and for cellular membrane composition and repair. Often taken as a lecithin supplement, choline is found in beef liver, egg yolk, peanuts, sunflower seeds, cauliflower and soy. Recent studies had suggested that choline intake during pregnancy might decrease the risk of spina bifida.. James Enaw, from the Texas A&M University System Health Science Center in Houston, Texas and colleagues from the California Birth Defects Monitoring Program, analyzed the presence of two specific variants of the genes encoding the enzymes human choline esterase A (CHKA) and CTP:Phosphocholine cytidylytransferase(PCYT1A) in 103 infants suffering from spina bifida and 338 ...
One hallmark of cancer is an increase of cellular phosphocholine (PC) and total choline-containing compounds (tCho), which are closely related to malignant transformation, invasion and metastasis. Enzymes in choline metabolism present attractive targets that can be exploited for treatment. Choline kinase (Chk) is a cytosolic enzyme that catalyzes the phosphorylation of choline (Cho) to phosphocholine (PC) by using ATP in the presence of magnesium. Over-expression of Chk-α has been observed in breast, prostate and lung cancers, making it an obvious choice for targeting. Down-regulation of Chk-α results in a significant reduction of cell proliferation and increased differentiation in highly invasive MDA-MB-231 human breast cancer cells after siRNA-Chk transfection. Another potential target is phosphatidylcholine-specific phospholipase D (PC-PLD). Two mammalian isoforms of PLD, PLD1 and PLD2 have currently been identified. PLD1 is activated by G proteins such as ARF, Rho and Rac. Elevated PLD1 ...
Title:A milder phenotype of megaconial congenital muscular dystrophy due to a novel CHKB mutation.,Author:De Fuenmayor-Fernández De La Hoz Carlos Pablo,Domínguez-González Cristina,Gonzalo-Martínez Jua...
Not all sources of choline are the same. Choline itself is the "basic" form (also referred to as "basic choline") and is usually purchased as "bitartrate." Basic choline is just that-the most "basic" and inexpensive form of choline. It is far less expensive than CDP-choline and as a result, it is used much more often in various supplements. Basic choline does not effectively cross the blood-brain barrier and doesnt reliably increase neural (brain) concentrations of choline. While the basic form of choline does have some benefits to your liver and may help with methylation, its benefits are definitely lacking when it comes to helping your brain.. Another basic form of choline is "choline citrate." This form can have about 50% more choline content than choline bitartrate and may have some other health benefits, such as helping to prevent fatty deposits from accumulating in the liver. Unfortunately, it is also ineffective at crossing the blood-brain barrier or increasing choline concentrations in ...
United States Patent Office 3,475,177 Patented Oct. 28, 1969 3,475,177 CHOLINE COMPOSITION Lawrence R. Jones, Terre Haute, Ind, assignor to Cemmercial Solvents Corporation, New York, N.Y., a corporation of Maryland No Drawing. Filed Feb. 9, 1966, Ser. No. 526,067 Int. (:1. A23k 1/00 US. Cl. 992 6 Claims ABSTRACT OF THE DISCLOSURE This invention relates generally to improved choline compositions. In a particular aspect it relates to choline salt and fat compositions. Choline salts are useful components of animal feeds. They are particularly useful in poultry feeds having a high fat content, e.g. a poultry feed having a fat content of above about 10%. One of the nutritional functions of choline is concerned with the metabolism and transportation of fat. A choline salt intended for use in animal feeds is usually supplied to the feed manufacturer in the form of a choline feed supplement prepared by mixing the choline salt with one or more of the dry ingredients of the feed preparation to act as a ...
Choline is a basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism. Choline is now considered to be an essential vitamin. While animals can synthesize small amounts (by converting phosphatidylethanolamine to phosphatidylcholine), it must be consumed in the diet to maintain health. Required levels are between 425 mg/day (female) and 550 mg/day (male). Milk, eggs, liver and peanuts are especially rich in choline. Most choline is found in phospholipids, namely phosphatidylcholine or lecithin. Choline can be oxidized to form betaine, which is a methyl source for many reactions (i.e. conversion of homocysteine e to methionine). Lack of sufficient amounts of choline in the diet can lead to a fatty liver condition and general liver damage. This arises from the lack of VLDL, which is necessary to transport fats away from the liver. Choline deficiency also ...
Your memory might be sharper if your diet contains lots of choline - a micronutrient found in some fatty foods, including egg yolks. A new study from Boston University School of Medicine suggests that adults whose choline intake was highest did better on memory tests than those whose intake was lowest; they were also less likely to have brain changes linked to dementia. The investigators reviewed data from 1,400 adults ages 36 to 83 who answered questions about their diets between 1991 and 1995. Later, between 1998 and 2001, the participants underwent MRI brain scans and tests of memory and other cognitive abilities. The differences seen between those with high and low choline consumption were small, but the studys lead author said the data suggested that participants with lower choline intakes were more likely to be on a "pathway" to mental decline than those with higher intakes. Another clue: choline is a precursor for acetylcholine, a neurotransmitter that plays a key role in memory and ...
Save 41% Jarrow Formulas - Citicoline CDP Choline 60 Capsules Citicoline CDP Choline Promotes Brain Function* 250 mg Cognizin Citicoline Citicoline [stabilized CDP Choline (cytidine 5diphosphocholine)] is a naturally occurring intermediate involved in the synthesis of phosphatidylcholine, a major constituent of the grey matter of brain tissue (30%).* Citicoline consumption promotes brain metabolism by enhancing the synthesis of acetyl-choline, restoring phospholipid content in the brain, which positively affects memory and other brain activity.*
Buy HealthAid Choline And Inositol at Rs. 1405 (Flat 20% off) from Nykaa. Check out HealthAid Choline And Inositol reviews. COD and Free Shipping ✓COD ✓Free Shipping
This eMedTV Web resource discusses choline fenofibrate, a drug used to treat unhealthy cholesterol and triglyceride levels. This article gives a brief overview of choline fenofibrate, with information on how to take it, safety warnings, and more.
Page 2 of 95 - GPC (choline), Uridine, DHA - posted in Brain Health: This combination of supps has been discussed before. I dont remember anyone feeling anything remarkable on it.
I came upon this remark (apart from things in the torah, the exodus book on when also the mispath i.e. civil law was given, that are called chok, as in can not be understood by mankind, like f.i. shabbos or the parable about the heifer that seems to make what is pure tamei, and what is tamei -unpure- pure.) that Rashi did not mention honour thy father and mother because it was self-evident to do, but at the same time leaving the puzzle as to when that was stated before or even whether that was so self-evident since i take it to mean keep what is good in your tradition and remember that. As in not everything was written down ...
Choline for ACh biosynthesis can potentially be supplied through three pathways: (1) de novo synthesis, (2) uptake of free choline by low- and high-affinity transporters, and (3) turnover of membrane phospholipids. Assessing the relative contributions of these pathways from the literature is somewhat difficult. Observations on the mouse CHT knockout suggest that choline uptake through the high-affinity transporter is critical for the synthesis of synaptically releasable pools of ACh (Ferguson et al. 2004). However, as noted earlier, researchers have also reported that a small, but significant fraction (∼20%) of ACh synthesis is independent of HC-3-sensitive choline uptake (Birks and Macintosh 1957; Guyenet et al. 1973). Other researchers have demonstrated that neuroendocrine cells are capable of synthesizing and releasing ACh in the absence of detectable CHT expression (Bauerfeind et al. 1993; Ferguson et al. 2003). These observations are generally attributed to the activity of low-affinity ...
Purpose: 11C-Choline positron emission tomography (PET) has been exploited to detect the aberrant choline metabolism in tumors. Radiolabeled choline uptake within the imaging time is primarily a function of transport, phosphorylation and oxidation. Rapid choline oxidation, however, complicates interpretation of PET data. In this study we investigated the biological basis of the oxidation of deuterated choline analogues and assessed their specificity in human tumor xenografts. Experimental Design: 11C-Choline, 11C-methyl-[1,2-2H4]-choline (11C-D4-choline) and 18F-D4-choline were synthesized to permit comparison. Biodistribution, metabolism, small-animal PET studies, and kinetic analysis of tracer uptake were performed in human colon HCT116 xenograft-bearing mice. Results: Oxidation of choline analogs to betaine was highest with 11C-choline, with reduced oxidation observed with 11C-D4-choline and substantially reduced with 18F-D4-choline; suggesting that both fluorination and deuteration were ...
BioAssay record AID 396975 submitted by ChEMBL: Displacement of [3H]DPCPX from human cloned adenosine A1 receptor expressed in CHOK1 cells by scintillation counting.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The aim of the present study was to investigate the function of human CTL1 in choline transport. The function of CTL1 as a choline transporter has been demonstrated in the Torpedo electric lobe (40), mouse (57), and rat (16, 40, 47). Human CTL1 was cloned and characterized as a cell-surface antigen in monocytes and differentiating dendritic cells; however, its choline transport was not clearly established (55). This study is the first to establish a functional link between the regulation of surface expression of CTL1 protein and choline transport in monocytes and differentiated macrophages. We realized that the well-known induction process of monocytic cell differentiation to macrophages by PMA is a good model for our study because the PMA/PKC system has the following properties: 1) they contribute to the differentiation processes, 2) they are common modulators of choline uptake in multiple systems, and 3) they are well-documented regulators of surface receptors and transporters (3, 10, 24, ...
Pregnant women may have added incentive to bulk up on broccoli and eggs: More choline during pregnancy can reduce a fetus response to stress and could cut a childs chances of developing hypertension and diabetes later in life. In a 12-week study led by Marie Caudill, professor of nutritional sciences, pregnant women who consumed approximately double the recommended 450-mg daily intake of choline in their third trimester had babies with 33 percent lower concentrations of cortisol-the hormone produced in response to stress that also increases blood sugar-compared with a control group of pregnant mothers taking the recommended daily amount. Choline is a major supplier of methyl (CH3) groups, which can attach to targeted regions of the genome and affect the level of a genes expression. Caudill credits the extra choline during pregnancy with easing the babys response to stress through changes in the methylation and expression patterns of the genes that regulate cortisol, which would be expected ...
Cholines function is similar to a vitamin. It is synthesized by the action of vitamin B12 and folic acid with amino acids methionine and glycine. However there is disagreement as to whether the amount would be adequate if there were none in the diet for a long period. If future researchers find a dietary need for it, it will be reclassified as a vitamin.. Choline functions with inositol as a basic constituent of a fatty substance called lecithin. It is water soluble and considered one of the B - complex vitamins and a lipotropic (fat emulsifier). It is present in the body of all living cells and widely distributed in animal and plant tissues.. Choline is used to make up acetylcholine, an important nerve transmitter which helps balance nervous activity. Acetylcholine plays an important part in memory and some studies have shown an increase in memory, intelligence and often helps those with premature senility when large amounts of choline and vitamin B5 are supplemented.. ...
Choline chloride is a common additive in animal feeds for both farm animals and pets. Choline is an essential nutritive chemical. Choline is important for cell membrane structure, for synthesizing folic acid and vitamin B12, and for protecting the liver from accumulating fat. ...
NOW Choline/Inositol has B-vitamins that support nerve transmission & assist in fatty acid metabolism. Choline & Inositol are essential components of all cells.
VitaDigest offer Choline & Inositol 100 Capsules 500 mg , Product Review For NOW Foods Formula and Information, Exclusive Reivews for Choline & Inositol 100 Capsules 500 mg NOW Foods
CDP Choline sodium capsules are a convenient way to increase choline levels in the brain without the bitter taste. Free Shipping for orders over $35.
Solgar : Choline / Inositol 250 / 250 Mg VegiCaps - 50 Ct. - Affordable Natural Supplements. Vitamins, minerals, herbals, supplements, and natural products. Free shipping on orders over $100!
November 26, 2012 -- Taking extra choline during pregnancy does not improve babies language and memory skills, according to a new study. I think eating the...
Stable and non-hygroscopic salts consisting of L-carnitine or alkanoyl-L-carnitine choline tartrate are disclosed. The salts are suitable for preparing solid compositions useful as dietary/nutritional supplements for human use and as fodder supplement for veterinary purposes.
Sporting Clay Chokes Sporting Clay chokes are made from 1704 stainless and precision machined to produce a choke tube that patterns better than standard chok
|strong||em|Result|/em||/strong|: Dramatically betters cell communication, nerve impulses, & liver bile detox. |strong||em|Benefit|/em||/strong|: ...
Merrit Kennedy is a reporter for The Two-Way, NPR's breaking news blog. She covers a broad range of issues, from the latest developments out of the
ЛОНДОН - Мы знаем, как искоренить полиомиелит. Действующая с 1980-х годов международная программа вакцинации под руководством Всемирной организации здравоохранения привела к почти полному исчезновению вируса полиомиелита. Болезнь, которая ежегодно убивала или парализовала полмиллиона людей на земле, поражает сегодня только несколько сот человек во всем мире.. Препятствие, стоящее сегодня на пути уничтожения вируса, не связано с медицинскими или техническими ограничениями, а вызвано политическим сопротивлением вакцинации. Действительно, ...
We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok ...
The study - published in the American Journal of Clinical Nutrition ​- points towards correlation between memory and dietary choline - found in such as saltwater fish, eggs, liver, chicken, milk and certain legumes, including soy and kidney beans - after researchers found that people with high intakes of choline performed better on memory tests, and were less likely to show brain changes associated with dementia. The researchers, led by senior researcher Rhoda Au of Boston University School of Medicine, USA, said that their results do not mean that choline is the answer to staving off Alzheimers disease, but noted that the findings do add to evidence that nutrition plays a role in the aging of the brain. However, Au cautioned against looking to any one nutrient as a magic bullet against dementia. Diet-dementia link​. A number of studies have reported links between diet and nutrition and the risk of developing Alzheimers disease. The authors noted that choline is the precursor to the ...
149 Feeding Rumen-Protected Choline to Transition Dairy Cows José Eduardo P. Santos a,1 and Fábio S. Lima b a Department of Animal Sciences b College of Veterinary Medicine University of Florida Introduction Choline [(CH 3)3N+CH 2 CH 2 OH], also known as trimethyl ethanolamine, is an essential .... ...
This product contains crystalline inositol and choline bound to tartaric acid for superior absorption. Our Choline & Inositol are carefully tested and produced to certified quality standards.
Buy Source Naturals Inositol & Choline 800mg 100 Tablets at Megavitamins Supplement Australia,Discount on volume available. Learn more - where to buy and what are the pros & Cons Inositol & Choline 800mg 100 Tablets.
Acetyl Choline Receptor Autoantibodies Blood - View Normal Values, Test Results, Procedure to conduct & Prices for Acetyl Choline Receptor Autoantibodies Blood | Practo
CDP Choline, also known as cytidine-diphosphate choline or citicoline, is a potent nootropic. It is best known for its ability to ...
Phosphatidylcholine is a phospholipid with a choline molecule attached and is the main form of choline found in food. Double Woods Phosphatidylcholine contains 210 x 1200 mg softgels. Click here to learn more.
References for Abcams Choline Acetyltransferase peptide (736-748) (ab45678). Please let us know if you have used this product in your publication
Irelands premier place to shop for Bluebonnet Nutrition Choline. eVitamins is a trusted and approved merchant to sell authentic Bluebonnet Nutrition items. See reviews, find coupons and more. Even get fast, reliable shipping to Ireland
I posted a picture of Kennedy, kat (his mom), Elton and me under the photo section and Joyce had asked about Kennedys MVD. I would like to say thank you to all of those that have offered help and guidence for my friend here at Cavalier Talk but I want to say just how helpful it was that I wrote some of this down and how that has led to where he is now. Just a recap, Kennedys main problem was that he would wake up scared and not able to breath. His vet said he had grade 3 and the MVD was
Carly Simon was surprised to learn that her pal, Jackie Kennedy Onassis, was willing to open up about some of the heartaches she endured with her first husband, President John F. Kennedy.
Duncan Kennedy News. Find breaking news, commentary, and archival information about Duncan Kennedy From The tribunedigital-chicagotribune
Ani and Choline CDP. I capped up some Choline to avoid the smell and taste. First day I only felt a mild good mood and a little clearer
Every city has its celebrated malcontents, and among San Franciscos finest were the Dead Kennedys. Led by the articulately confrontational…
Kennedy family said in a statement, "Weve lost the irreplaceable center of our family and joyous light in our lives, but the inspiration of his faith, optimism, and perseverance will live on in our hearts forever. He loved this country and devoted his life to serving it ...
Thanks to AI Sports, I had a chance to review HgH Pro. What is HgH Pro? AlphaSize (Alpha-Glycerol Phosphoryl Choline) (Alpha-GPC) 125 mg ** 500 mg **
A unique approach to connecting people and ideas in the life sciences, creating a space for probing conversations and deep insight into the topics and trends shaping our future.
BACKGROUND. The choline oxidation pathway comprises the sequential metabolism of choline into betaine, dimethylglycine (DMG), and sarcosine. In addition, dietary choline and betaine can be turned into trimethylamine N-amino oxide (TMAO). Alterations in choline metabolism may relate to cardiovascular disease (CVD) and type 2 diabetes (T2D). Several investigations have focused on systemic concentrations of choline and betaine; however large-scale prospective data are scarce. There is therefore a need for more comprehensive assessments of choline metabolites in relation to incident CVD, T2D and mortality, in addition to investigating any potential benefit in risk prediction from such biomarkers. AIM. We carried out observational cohort studies of the prospective relationships between plasma DMG and incident acute myocardial infarction (AMI) and mortality, as well as the association between systemic and urinary choline metabolites with the risk of incident T2D. The biomarkers impact on model ...
The report firstly introduced Choline Chloride basic information included Choline Chloride definition classification application industry chain structure industry overview; international market analysis, China domestic market analysis, Macroeconomic environment and economic situation analysis and influence, Choline Chloride industry policy and plan, Choline Chloride product specification, manufacturing process, product cost structure etc.. Browse Full Report with TOC @ http://www.qyresearchreports.com/report/global-and-china-choline-chloride-industry-2013-market-research-report.htm. Then statistics Global and China key manufacturers Choline Chloride capacity production cost price profit production value gross margin etc details information, at the same time, statistics these manufacturers Choline Chloride products customers application capacity market position Havayontact information etc company related information, then collect all these manufacturers data and listed Global and China Choline ...
2.7.1.1 Hexokinase 2.7.1.2 Glucokinase 2.7.1.3 Ketohexokinase 2.7.1.4 Fructokinase 2.7.1.5 Rhamnulokinase 2.7.1.6 Galactokinase 2.7.1.7 Mannokinase 2.7.1.8 Glucosamine kinase 2.7.1.9 Deleted entry 2.7.1.10 Phosphoglucokinase 2.7.1.11 6-phosphofructokinase 2.7.1.12 Gluconokinase 2.7.1.13 Dehydrogluconokinase 2.7.1.14 Sedoheptulokinase 2.7.1.15 Ribokinase 2.7.1.16 Ribulokinase 2.7.1.17 Xylulokinase 2.7.1.18 Phosphoribokinase 2.7.1.19 Phosphoribulokinase 2.7.1.20 Adenosine kinase 2.7.1.21 Thymidine kinase 2.7.1.22 Ribosylnicotinamide kinase 2.7.1.23 NAD(+) kinase 2.7.1.24 Dephospho-CoA kinase 2.7.1.25 Adenylyl-sulfate kinase 2.7.1.26 Riboflavin kinase 2.7.1.27 Erythritol kinase (D-erythritol 4-phosphate-forming) 2.7.1.28 Triokinase 2.7.1.29 Glycerone kinase 2.7.1.30 Glycerol kinase 2.7.1.31 Glycerate 3-kinase 2.7.1.32 Choline kinase 2.7.1.33 Pantothenate kinase 2.7.1.34 Pantetheine kinase 2.7.1.35 Pyridoxal kinase 2.7.1.36 Mevalonate kinase 2.7.1.37 Transferred entry: 2.7.11.1, 2.7.11.8, 2.7.11.9, ...

Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline...Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline...

... brucei choline/ethanolamine kinase 2), on the other hand, was found to be able to phosphorylate both ethanolamine and choline, ... was shown to be catalytically active as an ethanolamine-specific kinase, i.e. it had no choline kinase activity. The Km values ... Database mining revealed two putative C/EKs (choline/ethanolamine kinases) in the Trypanosoma brucei genome, which were cloned ... even though choline was the preferred substrate, with a Km 80 times lower than that of ethanolamine. The Km values for choline ...
more infohttps://omictools.com/4c6a385ea88700ca8fed3302f72c43d9-protocol

Choline/ethanolamine kinaseCholine/ethanolamine kinase

The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function ... Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/ ... due to choline kinase beta gene defects has been characterized.. 25740612. A novel silent variant in the choline kinase beta ... CHKB encodes choline kinase beta, an enzyme that catalyzes the first de-novo biosynthetic step of phosphatidylcholine, the most ...
more infohttps://pharos.nih.gov/idg/targets/Q9Y259

OriGene - CHKA (NM 001277) cDNA CloneOriGene - CHKA (NM 001277) cDNA Clone

Human choline kinase alpha (CHKA), transcript variant 1 available for purchase from OriGene - Your Gene Company. ... Domains: Choline_kinase. Gene Summary: The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline ... CHKA (untagged)-Human choline kinase alpha (CHKA), transcript variant 1, NM_001277.2, 10ug. $710. In Stock. ... Chka (untagged) - Mouse choline kinase alpha (cDNA clone MGC:65599 IMAGE:6841882), (10ug). $670. 3 weeks. ...
more infohttp://www.origene.com/cdna/trueclone/accession/NM_001277/SC110854.aspx

Myc-DDK-tagged ORF clone of Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S as transfection-ready DNA -...Myc-DDK-tagged ORF clone of Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S as transfection-ready DNA -...

Myc-DDK-tagged ORF clone of Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S as transfection-ready DNA - ... Functional kinase profile screening services * Protease screening & profiling * ELISAs & enzyme activity assays * Apoptosis ... home , products , origene , myc-ddk-tagged orf clone of homo sapiens choline o-acetyltransferase (chat), transcript variant s ... Myc-DDK-tagged ORF clone of Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S as transfection-ready DNA - ...
more infohttps://www.bioscience.co.uk/product~182258

Choline kinase - WikipediaCholine kinase - Wikipedia

Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first reaction in the choline ... which showed for the first time that choline kinase α can act as a protein chaperone (Asim et al., 2016 ;JNCI "Choline kinase ... Peisach, D; Gee, P; Kent, C; Xu, Z (2003). "The Crystal Structure of Choline Kinase Reveals a Eukaryotic Protein Kinase Fold". ... A related enzyme, ethanolamine kinase, tends to co-purify with choline kinase leading to a suggestion that the two activities ...
more infohttps://en.wikipedia.org/wiki/Choline_kinase

choline kinase activity Gene Ontology Term (GO:0004103)choline kinase activity Gene Ontology Term (GO:0004103)

The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
more infohttp://www.informatics.jax.org/vocab/gene_ontology/GO:0004103

Choline Kinase
     Summary Report | CureHunterCholine Kinase Summary Report | CureHunter

An enzyme that is active in the first step of choline phosphoglyceride (lecithin) biosynthesis by catalyzing the ... phosphorylation of choline to phosphorylcholine in the presence of ATP. Ethanolamine and its methyl and ethyl derivatives can ... Choline Phosphokinase; Choline Transphosphatase, ATP; Kinase, Choline; Phosphokinase, Choline; Transphosphatase, ATP Choline; ... Choline Kinase. Subscribe to New Research on Choline Kinase An enzyme that is active in the first step of choline ...
more infohttp://www.curehunter.com/public/keywordSummaryD002797-Choline-Kinase.do

CHKA - Choline kinase alpha - Homo sapiens (Human) - CHKA gene & proteinCHKA - Choline kinase alpha - Homo sapiens (Human) - CHKA gene & protein

Phosphorylates choline and ethanolamine. Has higher activity with choline. ... Choline kinase alpha (CHKA), Ethanolamine kinase 1 (ETNK1), Ethanolamine kinase 2 (ETNK2), Choline/ethanolamine kinase (CHKB) ... choline binding Source: Ensembl. *choline kinase activity Source: UniProtKB ,p>Inferred from Direct Assay,/p> ,p>Used to ... "Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine.". Malito E., Sekulic ...
more infohttps://www.uniprot.org/uniprot/P35790

Choline kinase expression patterns in human prostate cancers | Cancer ResearchCholine kinase expression patterns in human prostate cancers | Cancer Research

Selective choline kinase inhibitors may be useful in MRI/MRS studies to distinguish choline resonance produced by tumors that ... In this study, we evaluate the expression of choline kinase (the rate-limiting enzyme for new synthesis of choline containing ... Choline kinase expression patterns in human prostate cancers. Sandra M. Gaston, Dana L. Goldner, Dang Vu, Tendai L. Chizana, ... Choline kinase expression patterns in human prostate cancers. Sandra M. Gaston, Dana L. Goldner, Dang Vu, Tendai L. Chizana, ...
more infohttp://cancerres.aacrjournals.org/content/65/9_Supplement/895.3

Product Overview anti-Choline Kinase alpha AntibodiesProduct Overview anti-Choline Kinase alpha Antibodies

Selected quality suppliers for anti-Choline Kinase alpha antibodies. ... Order monoclonal and polyclonal Choline Kinase alpha antibodies for many applications. ... choline kinase alpha , choline kinase beta , choline kinase , CHETK-alpha , ethanolamine kinase , choline/ethanolamine kinase ... Show all anti-Choline Kinase alpha (CHKA) Antibodies with Pubmed References. * Human Polyclonal Choline Kinase alpha Primary ...
more infohttp://www.antibodies-online.com/abstract/Choline+Kinase+alpha+

Choline Kinase beta antibody | acris-antibodies.comCholine Kinase beta antibody | acris-antibodies.com

Alternative names for Choline Kinase beta antibody. Choline/ethanolamine kinase, CHKB, CHETK, CHKL, CKEKB, EKB, CKB, Choline ... Choline Kinase beta (transcript variant 1). Not available. Recombinant protein of human choline kinase beta (CHKB), transcript ... Background of Choline Kinase beta antibody. Kit Component:. - KN210253G1, CHKB gRNA vector 1 in pCas-Guide vector. - KN210253G2 ... Lenti ORF particles, CHKB (Myc-DDK tagged) - Human choline kinase beta (CHKB) , 200 uL, ,10^7 TU/mL. Not available. ...
more infohttps://www.acris-antibodies.com/target/choline-kinase-beta-antibody.htm

Abstract 46: Interdependence of choline kinase and phospholipase D in human breast cancer cells | Cancer ResearchAbstract 46: Interdependence of choline kinase and phospholipase D in human breast cancer cells | Cancer Research

Choline kinase (Chk) is a cytosolic enzyme that catalyzes the phosphorylation of choline (Cho) to phosphocholine (PC) by using ... Abstract 46: Interdependence of choline kinase and phospholipase D in human breast cancer cells. Balaji Krishnamachary, Mayur ... Abstract 46: Interdependence of choline kinase and phospholipase D in human breast cancer cells ... Abstract 46: Interdependence of choline kinase and phospholipase D in human breast cancer cells ...
more infohttp://cancerres.aacrjournals.org/content/70/8_Supplement/46

CHKA / CK / Choline Kinase Antibody (APC) for WB/Western LS-C513110CHKA / CK / Choline Kinase Antibody (APC) for WB/Western LS-C513110

Choline Kinase antibody LS-C513110 is an APC-conjugated rabbit polyclonal antibody to human Choline Kinase (CHKA / CK). ... Choline Kinase antibody LS-C513110 is an APC-conjugated rabbit polyclonal antibody to human Choline Kinase (CHKA / CK). ... Choline Kinase antibody LS-C513110 is an APC-conjugated rabbit polyclonal antibody to human Choline Kinase (CHKA / CK). ... Has higher activity with choline. Uniprot: P35790 NCBI:. NM_001277 NP_001268.2 Synonyms:. CHKA Antibody, Choline kinase alpha ...
more infohttps://www.lsbio.com/antibodies/chka-antibody-ck-antibody-choline-kinase-antibody-apc-wb-western-ls-c513110/526671

Silencing of Human Choline Kinase ExpressionSilencing of Human Choline Kinase Expression

Figure 2: Knockdown of choline kinase expression. Expression of ChoK in siRNA-transfected samples and controls was normalized ... In vitro chemically synthesized RNAi was utilized to knock down expression of the gene for human choline kinase (ChoK). This ... and choline kinase cDNA (b) from samples transfected with siRNAs against ChoK (ChoK siRNA 01 and 02) or control siRNA against ...
more infohttps://lifescience.roche.com/en_hk/articles/silencing-of-human-choline-kinase-expression.html

Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from...Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from...

Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from ... I have demonstrated that the increased phosphocholine arises through the induction of choline kinase activity. No increased ... Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from ... Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from ...
more infohttps://mcb.asm.org/content/9/1/325

Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer |...Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer |...

Aims Malignant transformation results in overexpression of choline-kinase (CHK) and altered choline metabolism, which is ... Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer ... Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer ...
more infohttp://jcp.bmj.com/content/68/9/703

N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulphonamide (H-89) inhibits incorporation of choline into phosphatidylcholine...N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulphonamide (H-89) inhibits incorporation of choline into phosphatidylcholine...

Instead, H-89 inhibited choline kinase, the enzyme catalysing the first step in the CDP-choline pathway. In the presence of 10 ... choline kinase activity was inhibited by 36 +/- 7.6% in vitro. Additionally, the phosphorylation of choline to phosphocholine ... inhibits incorporation of choline into phosphatidylcholine via inhibition of choline kinase and has no effect on the ... inhibits incorporation of choline into phosphatidylcholine via inhibition of choline kinase and has no effect on the ...
more infohttp://www.biochemj.org/content/297/1/241

Silencing of Human Choline Kinase ExpressionSilencing of Human Choline Kinase Expression

Figure 2: Knockdown of choline kinase expression. Expression of ChoK in siRNA-transfected samples and controls was normalized ... In vitro chemically synthesized RNAi was utilized to knock down expression of the gene for human choline kinase (ChoK). This ... and choline kinase cDNA (b) from samples transfected with siRNAs against ChoK (ChoK siRNA 01 and 02) or control siRNA against ...
more infohttps://lifescience.roche.com/en_be/articles/silencing-of-human-choline-kinase-expression.html

Choline/ethanolamine kinase family - WikipediaCholine/ethanolamine kinase family - Wikipedia

In molecular biology, the choline/ethanolamine kinase family includes choline kinase(EC 2.7.1.32) and ethanolamine kinase (EC ... Ethanolamine kinase has no choline kinase activity and its activity is inhibited by ADP. Inositol supplementation represses ... Kim KH, Voelker DR, Flocco MT, Carman GM (March 1998). "Expression, purification, and characterization of choline kinase, ... There are two putative choline/ethanolamine kinases (C/EKs) in the Trypanosoma brucei genome. ...
more infohttps://en.wikipedia.org/wiki/Choline/ethanolamine_kinase_family

Chkb - Choline/ethanolamine kinase - Mus musculus (Mouse) - Chkb gene & proteinChkb - Choline/ethanolamine kinase - Mus musculus (Mouse) - Chkb gene & protein

Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not ... Ethanolamine kinase 2 (Etnk2), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka) ... Choline/ethanolamine kinase. Alternative name(s):. Choline kinase beta (EC:2.7.1.32*Search proteins in UniProtKB for this EC ... Choline/ethanolamine kinaseAdd BLAST. 393. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ...
more infohttps://www.uniprot.org/uniprot/O55229

Regulation of choline kinase activity and phosphatidylcholine biosynthesis by mitogenic growth factors in 3T3 fibroblasts<...Regulation of choline kinase activity and phosphatidylcholine biosynthesis by mitogenic growth factors in 3T3 fibroblasts<...

Initial rates of choline transport were the same in quiescent and serum-treated cells, whereas choline kinase activity was 2- ... Initial rates of choline transport were the same in quiescent and serum-treated cells, whereas choline kinase activity was 2- ... Initial rates of choline transport were the same in quiescent and serum-treated cells, whereas choline kinase activity was 2- ... Initial rates of choline transport were the same in quiescent and serum-treated cells, whereas choline kinase activity was 2- ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/regulation-of-choline-kinase-activity-and-phosphatidylcholine-bio

ISMRM 2014) Role of Choline Kinase and Ethanolamine Kinase Isoforms in Modulating Phosphoethanolamine Levels in Breast Cancer...ISMRM 2014) Role of Choline Kinase and Ethanolamine Kinase Isoforms in Modulating Phosphoethanolamine Levels in Breast Cancer...

Role of Choline Kinase and Ethanolamine Kinase Isoforms in Modulating Phosphoethanolamine Levels in Breast Cancer Cells. Tariq ... Increased PC, but not PE, is observed in cells in culture because culture medium contains free choline, but no ethanolamine. We ... have used siRNAs to silence specific genes involved in choline and ethanolamine metabolism to understand their roles in ... additionally aggressive almost alone although approximately breast cancer cancers cell cells chemical chloroform choline ...
more infohttp://archive.ismrm.org/2014/1086.html

ISMRM 2010)  The
  Interdependence of Choline Kinase and Phospholipase D: Adaptation Mechanisms
  in Choline Phospholipid...ISMRM 2010) The Interdependence of Choline Kinase and Phospholipase D: Adaptation Mechanisms in Choline Phospholipid...

The Interdependence of Choline Kinase and Phospholipase D: Adaptation Mechanisms in Choline Phospholipid Metabolism of Human ... Downregulation of choline kinase (Chk) with siRNA results in increased phospholipase D1 (PLD1) expression and downregulation of ... A hallmark of cancer is an increase of cellular phosphocholine (PC) and total choline-containing compounds (tCho), which are ... These data support multiple targeting of enzymes in the choline pathway using a multiple siRNA approach.. ...
more infohttp://archive.ismrm.org/2010/0652.html

CHKB Gene - GeneCards | CHKB Protein | CHKB AntibodyCHKB Gene - GeneCards | CHKB Protein | CHKB Antibody

Choline Kinase Beta, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function ... Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/ ... CHKB (Choline Kinase Beta) is a Protein Coding gene. Diseases associated with CHKB include Muscular Dystrophy, Congenital, ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=CHKB&origene_rsshrna_trans=3

CHKB Gene - GeneCards | CHKB Protein | CHKB AntibodyCHKB Gene - GeneCards | CHKB Protein | CHKB Antibody

Choline Kinase Beta, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function ... Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/ ... CHKB (Choline Kinase Beta) is a Protein Coding gene. Diseases associated with CHKB include Muscular Dystrophy, Congenital, ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=CHKB&origene_full_func=6
  • homozygous or compound heterozygous mutations in the gene encoding choline kinase beta were identified in individulas with congenital muscular dystrophy. (nih.gov)
  • Clinical characteristics of megaconial congenital muscular dystrophy due to choline kinase beta gene defects in a series of 15 patients. (nih.gov)
  • New splicing mutation in the choline kinase beta (CHKB) gene causing a muscular dystrophy detected by whole-exome sequencing. (nih.gov)
  • A novel silent variant in the choline kinase beta causing muscular dystrophy. (nih.gov)
more