Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
New azasteroidal antifungal antibiotics from Geotrichum flavo-brunneum. III. Biological activity. (1/6)The A25822 antibiotic complex consists of seven biologically active factors. A comparative study of these factors determined that factor B possessed the greatest antifungal activity. The minimal inhibitory concentration of A25822B against isolates of Candida albicans was less than 0.3 similar to 5.0 mug/ml, Trichophyton mentagrophytes was inhibited at less than 0.0312 mug/ml. Other pathogenic fungi such as Cryptococcus neoformans, Histoplasma capsulatum, Blastomyces dermatitidis, Sporotrichum schenckii, and Microsporium gypseum were very susceptible to A25822B. Only limited antibacterial activity of A25822B was found. Parenteral or oral administration of 50 mg/kg of A25822B significantly extended the average survival time of mice infected with C. albicans. Doses of 20 mg/kg of A25822B caused a greater than ten-fold reduction in the number of Candida cells recovered from kidneys of infected mice. A solution of 0.5% or 0.25% A25822B applied topically was effective against an experimental dermatophyte infection on guinea pigs. A peak blood level of 3 mug/ml was achieved in mice following a 100 mg/kg dose of A25822B. Combination of A25822B with a polyene antibiotic in vitro showed antagonism. (+info)
New azasteroidal antifungal antibotics from Geotrichum flavo-brunneum. I. Discovery and fermentation studies. (2/6)Although Geotrichum species occur ubiquitously, antibiotic production by members of this genus has not previously been reported. The antibiotic complex designated A25822, consisting of one major and six minor structurally-related components active primarily against Candida and Trichophyton, represents a new family of naturally-occurring compounds. Approximately 90% of the antibiotic activity synthesized remained associated with the fungal cell mass, from which it was recovered by multiple methanolic extractions for quantitation. Antibiotic production was enhanced by tryptophan, iron, zinc, and high levels of dextrin. (+info)
New azasteroidal antifungal antibiotics from Geotrichum flavo-brunneum. II. Isolation and characterization. (3/6)A novel group of antibiotics, comprising microbiologically-active structurally-related factors A25822A, B, D, H, L, M and N, produced by culturing Geotrichum flavo-brunneum NRRL 3862 under submerged aerobic fermentation conditions was isolated by extraction. The individual factors were separated and purified by chromatography and crystallization. The major factor, A25822B, a 15-aza-24-methylene-D-homocholestadiene is a white crystalline compound, C25H45NO. The antibiotics are highly active against fungi and marginally active against bacteria. (+info)
Characterization of two steroidal ketones and two isoprenoid alcohols in dairy products. (4/6)Two steroidal ketones, delta-4-cholesten-3-one and delta-3,-5-cholestadiene-7-one, were isolated and identified for the first time in anhydrous milk fat and in nonfat dry milk. Together with these, two isoprenoid alcohols, phytol and dihydrophytol, were identified in anhydrous milk fat. Their identities were established on the basis of chromatographic and mass spectral data and confirmed by comparison with authentic materials. (+info)
Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice. (5/6)(+info)
Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2. (6/6)We evaluated the use of a modified phosphotungstic acid/MgCl2 precipitation procedure for the precipitation of apolipoprotein B-containing lipoproteins. Precipitation of these lipoproteins [very-low- and low-density lipoproteins, and lipoprotein (a)] is complete, with negligible coprecipitation of high-density lipoprotein subfractions (HDL1, HDL2, HDL3), even in hypertriglyceridemic sera. In comparison with ultracentrifugation, the precipitation method yields, on the average, values that are 0.17 mmol/L lower for cholesterol values but almost identical for apolipoprotein A-I and phosphatidylcholine. Looking for delta 3,5-cholestadiene formed from cholesterol in the precipitation residue, we used "high-performance" liquid chromatography and "high-performance" thin-layer chromatography and found none. (+info)
Phosphotungstic Acid in Vadodara,Phosphotungstic Acid Manufacturers and Suppliers from india
Business Directory for Phosphotungstic Acid Suppliers in Vadodara - Get contact details of Phosphotungstic Acid Manufacturers, Wholesale Phosphotungstic Acid Exporters, Best Phosphotungstic Acid Traders & Distributors Across the Vadodara.
12-phosphotungstic acid supported on SiO2
Page contains details about 12-phosphotungstic acid supported on SiO2 . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Anhydrous and water-assisted proton mobility in phosphotungstic acid - Fingerprint - [email protected]
Fingerprint Dive into the research topics of Anhydrous and water-assisted proton mobility in phosphotungstic acid. Together they form a unique fingerprint. ...
A computerized method of determining the number of synaptic contacts in a volume of cerebral cortex<...
TY - JOUR. T1 - A computerized method of determining the number of synaptic contacts in a volume of cerebral cortex. AU - West, Mark J.. AU - Coleman, Paul. AU - Wyss, Urs R.. PY - 1972/1/1. Y1 - 1972/1/1. N2 - A computerized flying‐spot scanning device has been used to count the number of synaptic contacts in neural tissue stained with ethanolic phosphotungstic acid (E‐PTA). The ability of the computer to idealize the irregular synaptic contacts as solid disks by modifying the information gleaned from scanning the preparation allows the stereological principle of Weibel & Gomez to be used to calculate the number of E‐PTA stained synaptic contacts per unit volume of tissue. This technique represents a significant improvement over the time necessary for making similar quantitative determinations manually. 1972 Blackwell Science Ltd. AB - A computerized flying‐spot scanning device has been used to count the number of synaptic contacts in neural tissue stained with ethanolic phosphotungstic ...
LOCALISATION MITOCHONDRIALE DENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE LESCARGOT | JCB
The mitochondrial derivative of the sperm cell of the Gastropod Helix is endowed with a compartment loaded with glycogen (Personne et André, 1964). The aim of this work is to establish whether this mitochondrial glycogen is synthesized in the mitochondrial derivative itself or elsewhere in the cell. For this purpose, living sperm were first incubated in a medium containing glucose 1-phosphate, then fixed, sectioned, and stained specifically for polysaccharides by the phosphotungstic acid technique or the periodic acid-thiosemicarbazide-silver proteinate technique. Comparison with controls shows that a synthesis of glycogen occurred during the time of incubation. It can be inferred from this result that an amylophosphorylase system controlling the metabolism of polysaccharides is present in the mitochondrial derivative itself. Results obtained with the iodine technique indicate that the original glycogen molecules are elongated during the experiment. It seems probable that the amylophosphorylase ...
Double Staining of Tobacco-mosaic-virus-infected Material Using Phosphotungstic Acid and Uranyl Acetate During Dehydration |...
The most widely used staining procedure in recent electron microscope studies of virus-infected plants has involved the use of uranyl acetate, either during dehydration or as a post-stain, together with lead citrate as a post-stain (Arnott & Smith, 1968; Milne & de Zoeten, 1967). Following the recent demonstration that the appearance of tobacco mosaic virus in thin section could be altered by prolonging the duration of dehydration staining with uranyl acetate (Cocking & Pojnar, 1968a), the use of other heavy-metal stains for dehydration staining, both alone and in combination with uranyl acetate, has been investigated. A double-staining procedure using phosphotungstic acid and uranyl acetate has been developed and the potential of this procedure in electron-microscope studies of virus infection is discussed. Phosphotungstic acid has been known for many years to give high contrast when used as a tissue stain during dehydration (Huxley, 1958).
Adhesion, proliferation and migration of NIH/3T3 cells on modified polyaniline surfaces
Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, ...
Most recent papers with the keyword immunohistochemistry heart valve | Read by QxMD
The small size of the adult and developing mouse heart poses a great challenge for imaging in preclinical research. The aim of the study was to establish a phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances the x-ray attenuation of soft-tissue to allow high resolution 3D visualization of mouse hearts by synchrotron radiation based μCT (SRμCT) and classical μCT. We demonstrate that SRμCT of PTA stained mouse hearts ex-vivo allows imaging of the cardiac atrium, ventricles, myocardium especially its fibre structure and vessel walls in great detail and furthermore enables the depiction of growth and anatomical changes during distinct developmental stages of hearts in mouse embryos ...
Surface structure and reactivity study of phosphotungstic acid-nitrogenated ormosils | SpringerLink
Supramolecular interactions between nitrogenated groups in hybrid ormosils bearing phosphotungstate nanocatalyst were used to tune the photocatalytical activity of these class-II hybrid materials obta
EXPERIMENTAL LIVER NECROSIS; I. THE HEXON BASES | JEM
1. The liver of the dog in which necrosis has been produced by injection of hæmatoxic immune sera is characterized in the less marked forms by a storing up of nitrogen in the persisting living cells, while in the diffuse forms the total nitrogen content is but slightly above the normal. This last is to be explained by the great diminution in persisting liver substance which limits the power of nitrogen accumulation.. 2. In all forms of necrosis there occurs an absolute increase of nitrogen precipitable by phosphotungstic acid (hexon bases) but the percentage increase, in relation to total nitrogen, diminishes in those forms (focal) in which the products of autolysis may be readily carried off by the blood stream and greatly increases in the diffuse form with large areas in which the circulation is seriously impaired.. 3. Although the absolute amount of nitrogen representing arginin and histidin varies, a relative increase is evident when this fraction is compared with the total ...
中国科学院上海有机化学研究所机构知识库(SIOC OpenIR): Reductive biotransformation of carbonyl compounds: Application of fungus, Geotrichum sp. G38...
Gu JX,Li ZY,Lin GQ. Reductive biotransformation of carbonyl compounds: Application of fungus, Geotrichum sp. G38 in organic synthesis[J]. TETRAHEDRON,1993,49(26):5805-5816 ...
INFLUÊNCIA DO pH NA PRODUÇÃO DE LIPASE EM MELAÇO DE SOJA POR Geotrichum candidum. - Blucher Proceedings
Atualmente, as lipases constituem o grupo mais importante de enzimas com enorme potencial de aplicação na área biotecnológica, principalmente no processo de...
Neurobiology of the aging brain: morphological alterations at synaptic regions<...
TY - JOUR. T1 - Neurobiology of the aging brain. T2 - morphological alterations at synaptic regions. AU - Bertoni-Freddari, C.. AU - Fattoretti, P.. AU - Casoli, T.. AU - Pieroni, M.. AU - Meier-Ruge, W.. AU - Ulrich, J.. PY - 1991. Y1 - 1991. N2 - Computer-assisted morphometric studies have been carried out on nerve cell terminal regions in rats of different ages. The numerical density (Nv), the average area (S) and the surface density (Sv) of ethanol phosphotungstic acid stained (E-PTA) synaptic junctions were evaluated in cerebellar glomeruli and dentate gyrus supragranular layers. The volume density (Vv), the average volume (V) and the numerical density (Nvm) of synaptic mitochondria were measured in the cerebellar glomeruli of young (3 months), adult (11 months) and old (28 months) animals. We found that during aging Nv and Sv undergo a significant decrease, whereas S is significantly increased. The mitochondrial Vv is unchanged in all the age groups analysed, whereas in old rats V is ...
Detection Of 16s RNA From Legionella Pneumophila Using Impedance Spectroscopy On Microfluidic Devices
Legionellosis is a life threatening disease caused by pathogenic Legionella pneumophila. L. pneumophila is found in natural and engineered water systems where ...
List of MeSH codes (D04)
... cholestadienes MeSH D04.808.247.222.222.347 - cholestadienols MeSH D04.808.247.222.222.347.200 - dehydrocholesterols MeSH ...
RCSB PDB - FUA Ligand Summary Page
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
glaucasterol Summary Report | CureHunter
List of MeSH codes (D04) - Wikipedia
Dihydrotachysterol | Harvard Catalyst Profiles | Harvard Catalyst
Primary Biliary Cirrhosis | The Korean Journal of Hepatology;: 364-372, 2006. | WPRIM
Autoimmune Diseases/diagnosis , Cholagogues and Choleretics/therapeutic use , Cholestadienes/administration & dosage , Cholic ... Cholestadienes / Cholic Acids / Prevalence / Liver Cirrhosis, Biliary Type of study: Prevalence study / Screening study ... Cholestadienes / Cholic Acids / Prevalence / Liver Cirrhosis, Biliary Type of study: Prevalence study / Screening study ...
Ergocalciferols | Profiles RNS
Moore RG, Lange TS, Robinson K, Kim KK, Uzun A, Horan TC, Kawar N, Yano N, Chu SR, Mao Q, Brard L, DePaepe ME, Padbury JF, Arnold LA, Brodsky A, Shen TL, Singh RK. Efficacy of a non-hypercalcemic vitamin-D2 derived anti-cancer agent (MT19c) and inhibition of fatty acid synthesis in an ovarian cancer xenograft model. PLoS One. 2012; 7(4):e34443 ...
MH DELETED MN ADDED MN
Search | Global Index Medicus
Comparative study of mupirocin versus topical fusidic acid in eradication of nasal carriage of Staphylococcus aureus in...
Lanosterol | Profiles RNS
Ergocalciferols | Profiles RNS
Zhang Y, Deb DK, Kong J, Ning G, Wang Y, Li G, Chen Y, Zhang Z, Strugnell S, Sabbagh Y, Arbeeny C, Li YC. Long-term therapeutic effect of vitamin D analog doxercalciferol on diabetic nephropathy: strong synergism with AT1 receptor antagonist. Am J Physiol Renal Physiol. 2009 Sep; 297(3):F791-801 ...