CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The rate dynamics in chemical or physical systems.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.
A narcotic antagonist similar in action to NALOXONE. It is used to remobilize animals after ETORPHINE neuroleptanalgesia and is considered a specific antagonist to etorphine.
Adherence of cells to surfaces or to other cells.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3.
An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Glycoproteins found on the membrane or surface of cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A cell line derived from cultured tumor cells.
Aquatic vertebrate sensory system in fish and amphibians. It is composed of sense organs (canal organs and pit organs) containing neuromasts (MECHANORECEPTORS) that detect water displacement caused by moving objects.
Studies in which variables relating to an individual or group of individuals are assessed over a period of time.
A peptide of about 22-amino acids isolated from the DUODENUM. At low pH it inhibits gastric motor activity, whereas at high pH it has a stimulating effect.
Cell surface proteins that bind gastrointestinal hormones with high affinity and trigger intracellular changes influencing the behavior of cells. Most gastrointestinal hormones also act as neurotransmitters so these receptors are also present in the central and peripheral nervous systems.
Cell surface receptors that bind specific neuropeptides with high affinity and trigger intracellular changes influencing the behavior of cells. Many neuropeptides are also hormones outside of the nervous system.
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
Drugs used for their effects on the gastrointestinal system, as to control gastric acidity, regulate gastrointestinal motility and water flow, and improve digestion.
A substituted benzamide used for its prokinetic properties. It is used in the management of gastroesophageal reflux disease, functional dyspepsia, and other disorders associated with impaired gastrointestinal motility. (Martindale The Extra Pharmacopoeia, 31st ed)
The region between the sharp indentation at the lower third of the STOMACH (incisura angularis) and the junction of the PYLORUS with the DUODENUM. Pyloric antral glands contain mucus-secreting cells and gastrin-secreting endocrine cells (G CELLS).
A plant genus of the family ASCLEPIADACEAE. Members contain steroidal glycosides and cytotoxic phenanthroindolizidine N-oxide alkaloids.
Unsaturated derivatives of PREGNANES.
The study of the physical and chemical properties of a drug and its dosage form as related to the onset, duration, and intensity of its action.
Proteins prepared by recombinant DNA technology.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

In this study the direct involvement of cadherins in adhesion-mediated growth inhibition was investigated. It is shown here that overexpression of N-cadherin in CHO cells significantly suppresses their growth rate. Interaction of these cells and two additional fibroblastic lines with synthetic beads coated with N-cadherin ligands (recombinant N-cadherin ectodomain or specific antibodies) leads to growth arrest at the G1 phase of the cell cycle. The cadherin-reactive beads inhibit the entry into S phase and the reduction in the levels of cyclin-dependent kinase (cdk) inhibitors p21 and p27, following serum-stimulation of starved cells. In exponentially growing cells these beads induce G1 arrest accompanied by elevation in p27 only. We propose that cadherin-mediated signaling is involved in contact inhibition of growth by inducing cell cycle arrest at the G1 phase and elevation of p27 levels.  (+info)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

The DNA repair protein apurinic endonuclease (APE/Ref-1) exerts several physiological functions such as cleavage of apurinic/apyrimidinic sites and redox regulation of the transcription factor AP-1, whose activation is part of the cellular response to DNA damaging treatments. Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towards AP-1, thus promoting DNA binding activity of AP-1. Inhibition of CKII mediated phosphorylation of APE/Ref-1 blocked mutagen-stimulated increase in AP-1 binding. It also abrogated the induction of c-Jun protein and rendered cells more sensitive to induced DNA damage. Thus, phosphorylation of APE/Ref-1 appears to be involved in regulating the different physiological activities of the enzyme. CKII mediated phosphorylation of APE/Ref-1 and concomitant increase in AP-1 binding activity appears to be a novel mechanism of cellular stress response, forcing transcription of AP-1 target gene(s) the product(s) of which may exert protective function.  (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located.  (+info)

Proteoglycan involvement in polyamine uptake. (4/15188)

We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake considerably, whereas chondroitin sulphate lyase had a limited effect. Inhibition of polyamine biosynthesis with alpha-difluoromethylornithine resulted in an increase of cell-associated heparan sulphate proteoglycans exhibiting higher affinity for spermine. The data indicate a specific role for heparan sulphate proteoglycans in the uptake of spermine by fibroblasts. Spermine uptake by pgsD-677, a mutant Chinese hamster ovary cell defective in heparan sulphate biosynthesis, was only moderately reduced (20%) compared with wild-type cells. Treatment of mutant cells with the above-mentioned xyloside resulted in a greater reduction of endogenous proteoglycan production as well as a higher inhibition of spermine uptake than in wild-type cells. Moreover, treatment with chondroitin sulphate lyase resulted in a selective inhibition of uptake in mutant cells, indicating a role for chondroitin/dermatan sulphate proteoglycans in the uptake of spermine by these cells. Fibroblasts, made growth-dependent on exogenous spermine by alpha-difluoromethylornithine treatment, were growth-inhibited by heparan sulphate or beta-D-xyloside, which might have future therapeutical implications.  (+info)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Serum-free culture filtrates of six Candida species and Saccharomyces cerevisiae were found to contain chemoattractants for human polymorphonuclear leukocytes (PMNs) and a mouse macrophage-like cell line, J774. The chemotactic factors differed for the PMN and J774 cells, however, in terms of heat stability, kinetics of liberation by the yeast cells, and divalent cation requirements for production. The chemoattractant in Candida albicans culture filtrates appeared to act through the formyl peptide receptor (FPR) of PMNs, since it was found to induce chemotaxis of Chinese hamster ovary (CHO) cells that were expressing the human FPR but did not induce chemotaxis of wild-type CHO cells. The C. albicans culture filtrates also induced migration of PMNs across confluent monolayers of a human gastrointestinal epithelial cell line, T84; migration occurred in the basolateral-to-apical direction but not the reverse direction, unless the epithelial tight junctions were disrupted. J774 cells did not migrate toward the formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albicans culture filtrate was not inhibited by an FPR antagonist (t-butoxycarbonyl-Met-Leu-Phe), suggesting that a different receptor mediated J774 cell chemotaxis. In conclusion, we have identified a receptor by which a non-serum-dependent chemotactic factor (NSCF) produced by C. albicans induced chemotaxis of PMNs. Additionally, we have shown that NSCF was active across epithelial monolayers. These findings suggest that NSCFs produced by C. albicans and other yeast species may influence host-pathogen interactions at the gastrointestinal tract mucosal surface by inducing phagocytic-cell infiltration.  (+info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology.  (+info)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen.  (+info)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein.  (+info)

BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
Microencapsulation of recombinant cells is a novel promising approach to tumor therapy in which therapeutic protein is sustainable and long-term delivered by microencapsulated cells. The semi-permeable membrane of microcapsule can protect cell from hosts immune rejection, increase the chemical stability of therapeutic protein and circumvent the problems of toxicity, limited half-lives and variation in circulating levels. Endostatin, a potent and specific angiogenesis inhibitor, could suppress the growth of primary and metastatic lesions in multiple murine tumor models. In this paper, APA microcapsules with high strength kept intact over 35 days and recombinant CHO cells kept the rapid proliferation viability and the continuous endostatin-expression function. The study of tumor treatment showed that the implantation of microencapsulated recombinant CHO cells decreased the neovascularization of tumor tissue by 59.4% and inhibited the B16 melanoma growth by 77.4%. Twenty days after tumor cell ...
BioAssay record AID 34248 submitted by ChEMBL: Displacement of [3H]NECA from human adenosine A2A receptor in stably transfected CHO cells.
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
serumm free culture of adherent cho cells - posted in Tissue and Cell Culture: Hi all, i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media for 72-96h. i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions which medium i can use to sustain 72-96h time? thnx
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. Biotechnol Bioeng. 2019 Aug 12 ...
Glenmark signs full commercial-use license for Horizons gene-edited CHO cells Cambridge, UK, 30 September 2019: Horizon Discovery Group plc (LSE: HZD) (Horizon), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase (GS) knockout Chinese Hamster Ovary (CHO) K1 cell line. Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Companys biomanufacturing processes. Martin Bertschinger, Deputy Director of Cell Sciences, Glenmark, explained: After extensive evaluation, Horizons GS knockout CHO K1 cell line demonstrated consistently impressive performance. We generated clones with high levels of productivity and a favorable stability profile relative to our previous system. Incorporating this technology into our ...
Phenotypic variations in the cells arise from changes in their DNA, including differences in methylation patterns. From analyzing CHO cell lines, researchers found that the transcriptome of each subclone also had a significant number of individual changes and that such changes indicate that epigenetic regulation is a hidden but important player in cell line development.
We have investigated the role of HIF-1 in the cellular response to redox modulation via the inhibition of oxidative phosphorylation. We demonstrate that manipulation of redox in air, achieved by inhibiting cytochrome oxidase with cyanide, induces HIF-1 mediated transcription in wild-type CHO and HT1080 human tumour cells but not in CHO cells deficient in the oxygen responsive, HIF-1alpha sub-unit of HIF-1. Hypoglycaemia attenuates cyanide-mediated transcription in non-transformed HIF-1 wild-type CHO cells but not the human tumour derived cell line. Cells lacking either HIF-1alpha, or the second composite sub-unit of HIF-1, HIF-1beta, were markedly more sensitive to the combined stress of perturbed redox and hypoglycaemia than wild-type cells. As such conditions together with hypoxia are prevalent in tumours, these data suggest that HIF-1 may have a protective role in adaptation to the tumour micro-environment. In support of this we demonstrate that HIF-1alpha deficient cells are less tumorigenic ...
We have investigated the role of HIF-1 in the cellular response to redox modulation via the inhibition of oxidative phosphorylation. We demonstrate that manipulation of redox in air, achieved by inhibiting cytochrome oxidase with cyanide, induces HIF-1 mediated transcription in wild-type CHO and HT1080 human tumour cells but not in CHO cells deficient in the oxygen responsive, HIF-1alpha sub-unit of HIF-1. Hypoglycaemia attenuates cyanide-mediated transcription in non-transformed HIF-1 wild-type CHO cells but not the human tumour derived cell line. Cells lacking either HIF-1alpha, or the second composite sub-unit of HIF-1, HIF-1beta, were markedly more sensitive to the combined stress of perturbed redox and hypoglycaemia than wild-type cells. As such conditions together with hypoxia are prevalent in tumours, these data suggest that HIF-1 may have a protective role in adaptation to the tumour micro-environment. In support of this we demonstrate that HIF-1alpha deficient cells are less tumorigenic than
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
name:조은석; Cho, Eun Suk; cho, eun suk; cho eun suk; eun suk cho; eun suk, cho; Cho, Eun Suk; Cho Eun Suk; Eun Suk Cho; Eun Suk, Cho
Feichtinger J., Hernandez I., Fischer C., Hanscho M., Auer N., Hackl M., Jadhav V., Baumann M., Krempl P.M., Schmidl C., Farlik M., Schuster M., Merkel A., Sommer A., Heath S., Rico D., Bock C., Thallinger G.G., Borth N. (2016) Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time. Biotechn. Bioeng. 113:10:2241-2253. DOI: 10.1002/bit.25990. Gludovacz E., Maresch D., Bonta M., Szöllösi H., Furtmüller P.G., Weik R., Altmann F., Limbeck A., Borth N., Jilma B., Boehm T. (2016) Characterization of recombinant human diamine oxidase (rhDAO) produced in Chinese Hamster Ovary cells. J Biotechnol. 227, 120-130. Klanert G., Jadhav V., Shanmukam V., Diendorfer A.m Karbiener M., Scheideler M., Bort JH., Grillari J., Hackl M., Borth N. (2016) A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines. J Biotechnol. DOI 10.1016/j.jiotec.2016.03.022. Priola J.J., Calzadilla N., Baumann M., Borth N., Tate ...
Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V. We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...
CHO-Anti-Human TSHR F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TSHR F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
CHO-Anti-Human TGF beta 2 scFv stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TGF beta 2 scFv gene to allow expression of the scFv. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more complex set-up needed.. In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. A comparison between Alternating Tangential Flow (ATF) and Tangential Flow Filtration (TFF) was performed using a recombinant CHO cell line producing a monoclonal antibody (mAb) as a model system. ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHO(BRI/rcTA)) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO(BRI/rcTA) pools producing high levels of recombinant proteins. Volumetric productivities of up to 900mg/L were reproducibly achieved for a Fc fusion protein and up to 350mg/L for an antibody after 14days post-induction in non-optimized fed-batch cultures. In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. ...
We have investigated whether the presence of a DNA repair enzyme, 06-methylguanine-DNA-methyltransferase (MGMT), affects the nature of spontaneous mutations in a mammalian cell line. We compared spontaneous mutations in the adenine phosphoribosyl transferase gene of a Chinese hamster ovary (CHO) cell line that expressed 14,000 MGMT molecules/cell with those in the parental CHO cells lacking this DNA repair activity. The mutation rate/cell/generation of the two CHO cell lines did not differ significantly. However, DNA sequence analysis of spontaneous mutations in the MGMT-proficient CHO cell line revealed a complex picture. No significant difference from the parental CHO cells was found in the number or type of deletions, frame-shifts, multiple substitutions, or insertions. The frequency of G:C to T:A transversions was elevated in MGMT-proficient CHO cells. Expression of the enzyme considerably reduced G:C to A:T transitions (25% versus 8.3%). This latter result is the first evidence that this ...
In a previous blog Strategies for Improving Antibody Production in CHO Cells three areas were identified where antibody production can be improved. In part one of the series titled Utilizing Gene Synthesis to Improve Antibody Production in CHO Cells, we looked at gene synthesis as an alternative to classic cloning that offers a precise way to create a gene. In part two of the series titled Strategies for Enhancing Media to Improve Antibody Production in CHO Cells, we examined the use of new media supplements to improve cell growth and productivity. In part three we will look at ways perfusion bioreactors can improve antibody production in CHO cell manufacturing.. In the biopharmaceutical industry there is an ongoing discussion about manufacturing capacity vs. demand for biologic drugs. Recently there has been increased concern because several biologic drugs have entered the final phases of the clinical pipeline. If many of these drugs receive approval, capacity could quickly become in ...
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. The Chinese hamster had been used in research since 1919 where they were used in place of mice for typing pneumococci. They were subsequently found to be excellent vectors for transmission of kala-azar (a.k.a. visceral leishmaniasis), facilitating leishmania research. In 1948, the Chinese hamster was first used in the United States for breeding in research laboratories. In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganians laboratory at the Boston Cancer Research Foundation and used it to ...
Kukkonen JP; G-protein-dependency of orexin/hypocretin receptor signalling in recombinant Chinese hamster ovary cells.; Biochem Biophys Res Commun, 2016 PubMed Europe PMC ...
Kukkonen JP; G-protein-dependency of orexin/hypocretin receptor signalling in recombinant Chinese hamster ovary cells.; Biochem Biophys Res Commun, 2016 PubMed Europe PMC Scholia ...
Glycosylation modulates growth, maintenance, and stress signaling processes. Consequently, altered N-glycosylation is associated with reduced fitness and disease. Therefore, expanding our understanding of N-glycans in altering biological processes is of utmost interest. Herein, clustered regularly interspaced short palindromic repeats/caspase9 (CRISPR/Cas9) technology was employed to engineer a glycosylation mutant Chinese Hamster Ovary (CHO) cell line, K16, which expresses predominantly hybrid type N-glycans. This newly engineered cell line enabled us to compare N-glycan effects on cellular properties of hybrid type N-glycans, to the well-established Pro´5 and Lec1 cell lines, which express complex and oligomannose types of N-glycans, respectively. Lectin binding studies revealed the predominant N-glycan expressed in K16 is hybrid type. Cell dissociation and migration assays demonstrated the greatest strength of cell-cell adhesion and fastest migratory rates for oligomannose N-glycans, and ...
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells. (1981). Subject Strain Bibliography 1981. 19 ...
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In interphase Chinese hamster ovary (CHO) cells, the centrosome is attached to the nucleus very firmly. This nuclear-centrosome complex is isolated as a coherent structure by lysis and extraction of cells with Triton X-100 in a low ionic strength medium. Under these conditions, the ultrastructure of the centrioles attached to the nucleus can be discerned by electron microscopy of whole-mount preparations. The structural changes of the centrioles as a function of the cell cycle were monitored by this technique. Specifically, centriolar profiles were placed into six categories according to their orientation and the length ratio of daughter and parent centrioles. The proportion of centrioles in each category was plotted as a frequency histogram. The morphological changes in the centriole cycle were characterized by three distinguishable events: nucleation, elongation, and disorientation. The progress of centrioles through these stages was determined in synchronous populations of cells starting from ...
BGI, the giant genomics institute located in Shenzhen, and GT Life Sciences of San Diego have published their collaborative study on the genomic sequence of the Chinese hamster ovary (CHO) K1 cell line in Nature Biotechnology. Over 70% of the recombinant therapeutic proteins sold today are manufactured using mammalian cells, primarily CHO cell lines. GT Life Sciences uses a metabolic modeling platform to design new products and processes for the life sciences field. It says a better understanding of the genome will speed development of new recombinant protein therapies. More details.... Share this with colleagues:   var switchTo5x=true;stLight.options({publisher:d7871f5b-67bc-4d30-b66f-1465d0b97213});
Previous findings have established G-6-P as an important mediator promoting HKII dissociation from mitochondria to cytosol (White and Wilson, 1990; Aleshin et al., 1998; Sebastian et al., 1999). In CHO cells overexpressing HKII, but not HKI, we found that glucose removal was associated with a delay in the subsequent rate of glucose phosphorylation (when Cyto B was present to block glucose efflux; John et al., 2011). Based on our prior detailed analysis in CHO cells (John et al., 2011), we attribute this delay to the activation of glycogenolysis when glucose was removed, which elevated G-6-P levels to both inhibit HKII and promote its dissociation from mitochondria. This interpretation was supported by our findings that IAA, which elevates G-6-P by inhibiting glycolysis distally (Fig. S2), caused HKII to dissociate from mitochondria in intact CHO cells with glucose present, and that exogenous G-6-P accelerated dissociation of HKs from mitochondria in permeabilized CHO cells (John et al., ...
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
We have isolated cis-diamminedichloroplatinum(II) (CDDP)-resistant variants, C/CDP-1 and C/CDP-2, from a Chinese hamster ovary (CHO) cell line after a stepwise exposure to increasing concentrations of CDDP, and a CDDP-sensitive revertant, R-1, from C/CDP-2 after continuous incubation for 5 months in the absence of CDDP. C/CDP-1 and C/CDP-2 showed 7- and 10-fold higher resistance to CDDP, respectively, compared to CHO cells. C/CDP-2 was cross-resistant to carboplatin, l-phenylalanine mustard (melphalan), and CdSO4, but not to other anticancer agents. Alkaline elution of DNA showed an increased amount of DNA interstrand cross-linking formation in CHO cells, but not in C/CDP-2 cells, when CHO and C/CDP-2 cells were cultured with CDDP. By contrast, alkaline elution of DNA showed increased formation of DNA cross-links when nuclei of C/CDP-2 cells were treated with CDDP. The activity of glutathione S-transferase (GST) of C/CDP-1 and C/CDP-2 was 4- and 6-fold higher than that of CHO cells, ...
The technology is set to heighten the companys GPEx expression platform through the utilization of a glutamine synthase knock-out Chinese hamster ovary cell line.
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Activin A, Human, Recombinant, CHO Cells Activin A, Human, Recombinant, is a member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. A disulfide-linked homodimer of two 116-a.a. βA subunits. - Find MSDS or SDS, a COA, data sheets and more information.
Over the past decades, the increase in maximal cell numbers for the production of mammalian derived biologics has been in a large part due to the development of optimal feeding strategies. Engineering of the cell line is one of probable approaches for increasing cell numbers in bioreactor. We have demonstrated that the over-expression of the c-myc gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the control. The changes were attributed to a rapid transition into S-phase from a shortened duration of G1 phase and to the uncoupling of cell size from cell proliferation. To achieve the |70% increase in maximal cell density without additional supply of nutrients the cells underwent an overall reduction of 14% in size as well as a significant decrease in glucose and amino acid consumption rate. Consequently, the total biomass accumulation did not show a significant change from the control. The amount of hSEAP-hFc activity of the over
Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells Academic Article ...
Wong, D.C.F., Wong, K.T.K., Nissom, P.M., Yap, M.G.S., Heng, C.K. (2006). Targeting early apoptotic genes in batch and fed-batch CHO cell cultures. Biotechnology and Bioengineering 95 (3) : 350-361. [email protected] Repository. https://doi.org/10.1002/bit. ...
LinkedIn: Chinese Hamster Ovary cells, known as CHO, play an important role in modern medicine. NISTs new CHO peptide library will enable better production of treatments for psoriasis, cancer, hemophilia and leukemia.
In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. Optimised protein expression protocols provide the ability to load cells with greater quantities of DNA relative to the standard CHO protocol. As a result, average protein expression per cell is increased.
One Liter of Irvine Scientific BalanCD™ CHO Growth A Medium for use with the CELLine bioreactor flask |p| BalanCD CHO™ Growth A. It is a chemically-defined, no animal- derived media specifically designed for used in CHO cell applications. It
There are now several examples of single G protein-coupled receptors to which binding of specific agonists causes differential effects on the associated signaling pathways. The dopamine D2 receptor is of special importance because the selective activation of functional pathways has been shown both in vitro and in situ.
BalanCD® CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...
BalanCD® CHO Feed 1 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...
About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
N,N,N,N-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.
We have isolated three independent Chinese hamster ovary cell mutants (B3853, I223, and M311) with temperature-sensitive, pleiotropic defects in receptor-mediated endocytosis. Activities affected at 41 degrees C include uptake via the D-mannose 6-phosphate receptor, accumulation of Fe from diferric transferrin, uptake of alpha 2-macroglobulin, compartmentalization of newly synthesized acid hydrolases, resistance to ricin, and sensitivity to diphtheria and Pseudomonas toxins and modeccin. The three mutants also displayed decreased sialylation of some secreted glycoproteins at 41 degrees C, reminiscent of the nonconditional mutant DTG1-5-4 that showed both endocytic and Golgi-associated defects (Robbins, A.R., C. Oliver, J.L. Bateman, S.S. Krag, C.J. Galloway, and I. Mellman, 1984, J. Cell Biol., 99:1296-1308). Phenotypic changes were detectable within 30 min after transfer of the mutants to 41 degrees C; maximal alteration of most susceptible functions was obtained 4 h after temperature shift. At ...
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks. ...
TY - JOUR. T1 - Erythropoietin fails to reverse the anemia in mice continuously exposed to tumor necrosis factor-alpha in vivo. AU - Clibon, U.. AU - Bonewald, Lynda. AU - Caro, J.. AU - Roodman, G. David. PY - 1990. Y1 - 1990. N2 - Tumor necrosis factor-α (TNF) is a monokine produced by activated macrophages that has cytotoxic and cytostatic effects on erythroid progenitor cells. We have recently shown that Chinese hamster ovary cells transfected with the human TNF gene and which constitutively express TNF induced a hypoproliferative anemia, mild thrombocytopenia, and mild leukocytosis when injected into nude mice. We have used this murine model to determine if treatment with recombinant human erythropoietin can prevent or ameliorate the anemia seen with long-term continuous exposure to high concentrations of TNF. Mice bearing TNF-producing tumors became anemic with hematocrits ranging from 30 to 32%. Treatment with recombinant human erythropoietin (100-1000 U/kg body weight three times per ...
The production of biopharmaceuticals for human use began in 1982 with recombinant insulin, and the development of new biopharmaceuticals has grew almost exponentially ever since. Over the past two decades, Chinese hamster ovary (CHO) cells have become the standard mammalian host cell line, with the expression and production of nearly 70% of all biopharmaceuticals [1, 2]. CHO cells provide efficient post-translational modifications, which allow the production of recombinant proteins with glycoforms that are both compatible with, and bioactive in humans [1]. CHO cells can also be easily manipulated genetically, which has become of great importance more recently [3]. These two characteristics are especially important in the production of biosimilars, where achieving the correct extent of similarity to the reference molecule is a great challenge. The nucleotide sequence of the gene that encodes amino-acid sequence of the desired protein is the same as for the reference molecule. In contrast, ...
TY - JOUR. T1 - One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells. AU - Yang, Ganglong. AU - Höti, Naseruddin. AU - Chen, Shao Yung. AU - Zhou, Yangying. AU - Wang, Qiong. AU - Betenbaugh, Michael. AU - Zhang, Hui. PY - 2020/4/17. Y1 - 2020/4/17. N2 - Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. Moreover, an increased ...
TY - CONF. T1 - Engineering amino acid supply pathways in Chinese hamster ovary cells. AU - Ley, Daniel. AU - Lund, Anne Mathilde. AU - Rank, Julie. AU - Kildegaard, Helene Faustrup. AU - Andersen, Mikael Rørdam. N1 - Conference code: 9. PY - 2014. Y1 - 2014. M3 - Poster. T2 - 9th Danish Conference on Biotechnology and Molecular Biology. Y2 - 22 May 2014 through 23 May 2014. ER - ...
Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy.
Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer rate. In this study, a Chinese hamster ovary cell line producing a recombinant antibody was cultivated in a 5L OSR and a 3L STR, both operated with or without pH control. Effects of bioreactor type and pH control on cell growth and metabolism and on recombinant protein production and glycosylation were determined. In pH-controlled bioreactors, the glucose consumption and lactate production rates were higher relative to cultures grown in bioreactors without pH control. The cell density and viability were higher in the OSRs than in the STRs, either with or without pH control. Volumetric recombinant antibody yields were not affected by the process conditions, and a glycan analysis of the antibody by mass spectrometry did not reveal major ...
To address the needs of the growing antibody market, it is crucial to develop robust and efficient fed-batch cell culture processes that can ensure high product yields and quality. In this work, we have investigated the combined effect of both process and metabolic engineering strategies on the N-glycosylation state of an antibody. To this end, we have used two CHO cell clones exhibiting distinct phenotypes and performed fed-batch cultures employing two concentrated feed formulations, allowing us to infer the relative impact of these changes on antibody productivity and product quality. The comparative study was done with a parental CHO cell line and one clonal derivative stably expressing the PYC2 gene and characterized by a significantly altered lactate metabolism. We have also assessed these strategies in combination with or without manganese and galactose addition, two culture supplements commonly employed to modulate the glycosylation profile of antibodies. A comparative study of the resulting
Glycosylation enormously influences the security and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to outline optimum cell tradition feeding methods that management rTP glycosylation, its essential to know the way nucleotide sugars (NSs) are consumed in direction of host cell and rTP glycosylation.. Here, we current a theoretical framework that integrates the reported glycoproteome of CHO cells, the quantity of N-linked and O-GalNAc glycosylation websites on particular person host cell proteins (HCPs), and the carbohydrate content material of CHO glycosphingolipids to estimate the demand of NSs in direction of CHO cell glycosylation. We have recognized the most plentiful N-linked and O-GalNAc CHO glycoproteins, obtained the weighted frequency of N-linked and O-GalNAc glycosites throughout the CHO cell proteome, and have derived stoichiometric coefficients for NS consumption in direction of CHO cell glycosylation.. ...
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and plasticity in the context of genetic alterations.
LI- and Ksp-cadherin, the members of the 7D-cadherin-family, exhibit distinct structural features which make them unique within the cadherin superfamily. In this thesis, the adhesive properties of 7D-cadherins were analyzed on the cellular and single molecule level. Ksp-cadherin mediates a specific and Ca2+-dependent cell-cell adhesion in transfected CHO cells comparable to that of LI- and E-cadherin. In contrast to E-cadherin, the 7D-cadherins could not be co-immunoprecipitated with beta-catenin. This indicates that Ksp-cadherin like LI-cadherin might not be linked to the cytoskeleton via beta-catenin. Analyzing a fusion protein consisting of the entire extracellular part of LI- cadherin fused to the Fc-part of the human IgG1 (LI-Fc) with affinity chromatography and atomic force microscopy revealed a low affinity homotypic interaction (KD of 27 µM), a short lifetime (1.4 s) and a low unbinding force (27-51 pN at retrace velocities of 150-3000 nm/s). Binding is regulated by low-affinity Ca2+ ...
Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell fed-batch culture processes. Cytotechnology, 69(1), 39-56. doi: 10.1007/s10616-016-0036-5 ...
Incubation with 2N-methyl-9-hydroxyellipticinium (NMHE) increases the killing of gamma-irradiated CHO cells. The main effect is observed in the case of exponentially growing cells which are also more sensitive to the drug alone than plateau phase cel
Cell adhesion is vastly important in cancer metastasis since the cells can only migrate once they have adhered and at some point become unbound from the extracellular matrix (ECM). The purpose of this experiment was to assess the amount of adhesion that occurs between cells and an ECM using various concentrations of the ECM. Several assays were performed with Chinese Hamster Ovary (CHO) cells to obtain a control. CHO cells have the integrin 51 receptors that allow the cells to adhere to the ligand Fibronectin in the ECM. B7 cells are CHO cells that have been stably transfected with the integrin IIb3. Since B7 cells contain the integrin IIb3 in addition to integrin 51, Fibrinogen was used as the ECM. Serum-starved cells were labeled with Calcein AM and readings were taken at an absorbance of 485 nm directly correlating to the amount of cells adhered to the ECM. To test the role of CIB1 in cell adhesion the B7 cells were transfected with Mock-pcDNA and CIB1 DNA. The ...
The test substace was evaluated for potential genotoxic activity using the Sister Chromatid Exchange (SCE) test. Preliminary experiments were performed with CHO cells to determine an appropriate range of test concentrations in which the highest concentration would kill no more than (approximately) 90% of the treated cells. Test concentrations for the SCE test were chosen in a range of 40 to 60% growth inhibition so that sufficient numbers of cells in the second division (M2) would be available for determination of SCEs. Test results with DEGHE indicated that concentrations of 3 mg/ml or higher were lethal to CHO cells. The highest suitable concentrations tested were 2.0 mg/ml without S9 and 2.5 rng/ml with S9 activation for the CHO test. For the SCE test maximum concentrations tested were 2.0 mg/ml both with S9 and without S9 activation. For determination of direct genotoxic action, CHO cells were exposed to DEGHE and appropriate controls for 5 hours without S9 activation. Indirect activity, ...
Two Chinese hamster ovary (CHO) cell variants differ substantially in their sensitivity to N-methyl-N -nitro-N-nitrosoguanidine (MNNG). The resistant clone (Cl 3) was isolated from the sensitive...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Rolling of GP Ibα-expressing cells on immobilized P-selectin. (A) CHO cells expressing either the GP Ib-IX complex or only GP Ibβ and GP IX were allowed to
I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am interested in E. coli and CHO (Chinese Hamster Ovary cells), but any reference to studies of this kind for any typical cell will be useful.. Ill accept an answer containing a representative sample of references to the literature on this subject. Preferably recent papers (since 2010).. If you can provide the times spent in each phase but dont have references at hand, that will also be useful.. ...
A research team at the Northeast Agricultural University in Harbin managed to breed three transgenic pigs by injecting fluorescent green protein and a bunch of other junk into embryonic pigs, said Professor Liu Zhonghua. Liu wore a fancy white lab coat and had multiple degrees adorning his walls, so we assume he must be pretty smart ...
CHO cells[edit]. DHFR lacking CHO cells are the most commonly used cell line for the production of recombinant proteins. These ... which are important for cell proliferation and cell growth.[14] DHFR plays a central role in the synthesis of nucleic acid ... regulation of transcription involved in G1/S transition of mitotic cell cycle. • tetrahydrofolate metabolic process. • ... DHFR is responsible for the levels of tetrahydrofolate in a cell, and the inhibition of DHFR can limit the growth and ...
Non-human mammalian expression systems such as CHO or NS0 cells have the machinery required to add complex, human-type glycans ... Within the immune system the N-linked glycans on an immune cell's surface will help dictate that migration pattern of the cell ... whereas human cells only produce glycoproteins containing N-acetylneuraminic acid. Furthermore, animal cells can also produce ... The nature of N-linked glycans attached to a glycoprotein is determined by the protein and the cell in which it is expressed. ...
The clastogen-suppressing effects of Tochu tea in CHO cells and mice. „Mutation research". 388 (1), s. 7-20, 1997. DOI: 10.1016 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line.. „Cancer Research". 61 (15), s. 5749 ... Scopoletin suppresses pro-inflammatory cytokines and PGE2 from LPS-stimulated cell line, RAW 264.7 cells.. „Fitoterapia". 75 (3 ... Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor kappaB and caspase-3 ...
"The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells". Proc Natl Acad Sci U S A. ... "Role of Fanconi DNA repair pathway in neural stem cell homeostasis". Cell Cycle. 7 (13): 1911-5. doi:10.4161/cc.7.13.6235. PMID ... Cell. 7 (2): 249-62. doi:10.1016/s1097-2765(01)00173-3. PMID 11239454. Yang Y, Kuang Y, Montes De Oca R, Hays T, Moreau L, Lu N ... Cell. Biol. 19 (7): 4866-73. doi:10.1128/mcb.19.7.4866. PMC 84285 . PMID 10373536. Park SJ, Ciccone SL, Beck BD, Hwang B, Freie ...
"Evidence obtained by segregation analysis for functional hemizygosity at the Emtr locus in CHO cells". Cell. 14: 1007-1013. doi ... In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ... A chromosome in a diploid organism is hemizygous when only one copy is present.[2] The cell or organism is called a hemizygote ...
Nominal cell voltage. cell voltage varies nonlinearly in the range 2.5-1.7 V during discharge; batteries often packaged for 3 V ... Jeong, S. S.; Lim, Y.; Choi, Y. T.; Kim, K. W.; Ahn, H. J.; Cho, K. K. (2006). "Electrochemical properties of lithium sulfur ... Polysulfides are reduced on the cathode surface in sequence while the cell is discharging: S. 8 → Li. 2S. 8 → Li. 2S. 6 → Li. 2 ... Across a porous diffusion separator, sulfur polymers form at the cathode as the cell charges: Li. 2S → Li. 2S. 2 → Li. 2S. 3 → ...
"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ...
Am J Respir Cell Mol Biol. 45:88-94. Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... Am J Respir Cell Mol Biol. 41:661-70 Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... 57:51-9. Tang PS, Mura M, Seth R, Liu M. (2008) Acute lung injury and cell death: how many ways can cells die? Am J Physiol 294 ... There are two types of alveolar epithelial cells - Type 1 pneumocytes represent 90% of the cell surface area, and are easily ...
5-HT2B and 5-HT2C receptors in CHO-K1 cells". British Journal of Pharmacology. 128 (1): 13-20. doi:10.1038/sj.bjp.0702751. PMC ...
... the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell ... "Cell. 136 (2): 272-83. doi:10.1016/j.cell.2008.11.047. PMC 2859625. PMID 19167329.. ... "J. Cell Biol. 161 (4): 715-25. doi:10.1083/jcb.200301043. PMC 2199356. PMID 12756234.. ...
The main focus of his experimental work was the epigenetic control of gene expression by DNA methylation in CHO cells. These ... The double life of Holliday junctions Cell Research (2010) 20:611-613. doi: 10.1038/cr.2010.73; published online 25 May 2010 ... and this has now become documented as a basic epigenetic mechanism in normal and also cancer cells. In 1988 he moved to a ... experiments provide direct evidence that DNA methylation is a primary cause of gene silencing in mammalian cells. ...
Expression of single isoforms in heterologous systems such as human embryonic kidney (HEK) cells, Chinese hamster ovary (CHO) ... HCN channels are thought to consist of four either identical or non-identical subunits that are integrally embedded in the cell ... Wahl-Schott, C; Biel, M (Feb 2009). "HCN channels: structure, cellular regulation and physiological function". Cell Mol Life ... cells and Xenopus oocytes yield homotetrameric channels able to generate ion currents with properties similar to those of the ...
Insertion of a target cassette in a mammalian host cell line (CHO DG44 in suspension culture) and exchang with an ER stress ... but it also finds increasing use for the systematic generation of stem cells. Stem cells can be used to replace damaged tissue ... Cell. Biol. 26 (2): 605-616. doi:10.1128/MCB.26.2.605-616.2006. PMC 1346909. PMID 16382151. Branda, C.S.; Dymecki, S.M. (2004 ... Develop". Developmental Cell. 6 (1): 7-28. doi:10.1016/S1534-5807(03)00399-X. PMID 14723844. Oumard, A.; Qiao, J.; Jostock, T ...
2003). "alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP- ... positive regulation of cell proliferation. • cell proliferation. • positive regulation of smooth muscle contraction. • signal ... cell-cell signaling. • DNA metabolic process. • multicellular organism development. • adenylate cyclase-activating adrenergic ... Cell Genet. 66 (3): 170-1. doi:10.1159/000133693. PMID 8125015.. *. Forray C, Bard JA, Wetzel JM, et al. (1994). "The alpha 1- ...
Thielemans L, Depoortere I, Vanden Broeck J, Peeters TL (2002). "The motilin pharmacophore in CHO cells expressing the human ... Thielemans L, Depoortere I, Van Assche G, et al. (2001). "Demonstration of a functional motilin receptor in TE671 cells from ...
Agonist-induced internalization and desensitization of the human nociceptin receptor expressed in CHO cells.". Cell. Mol. Life ... receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines.". Brain Res. Mol. Brain Res. 32 (2): ...
"Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis". Cell. Elsevier BV. 14 (4): ... In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... "Molecular Cell Biology (4th ed.).. *^ Gupta, Radhey S.; Chan, David Y.H.; Siminovitch, Louis (1978). " ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ...
Cell. 1996 Jun 28; 85 (7) :1037-46 [49] Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating ... Cell. 1994 Apr 8; 77 (1) :53-62 [48] Sterol-regulated release of SREBP-2 from cell membranes requires two sequential cleavages ... Cell. 1975 Nov; 6 (3) :307-16. [3] Release of low density lipoprotein from its cell surface receptor by sulfated ... a possible trigger for degradation of HMG CoA reductase and crystalloid endoplasmic reticulum in UT-1 cells. Cell. 1984 Apr; 36 ...
Cho SW, Kim S, Kim JM, Kim JS (March 2013). "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease ...
Another form of rhG-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). As this is a mammalian ... White blood cells. The G-CSF-receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G- ... Stem cell transplantsEdit. G-CSF may also be given to the receiver in hematopoietic stem cell transplantation, to compensate ... positive regulation of cell proliferation. • neutrophil differentiation. • macrophage differentiation. • myeloid cell ...
Thereafter 1-6 µg of plasmidial DNA is transfected by electroporation into QMCF cells (CHO or HEK293 based cells) for ... QMCF cell lines express Large-T antigen and EBNA-1 proteins which bind the viral sequences on the QMCF plasmid and hence ... Compared to usage of stable cell line, QMCF technology is a rapid method leaving out time-consuming clone selection step during ... Since QMCF plasmids contain antibiotic resistance gene and are able to stably replicate and remain inside dividing cells, a ...
... endogenous ligands were isolated from cells (HEK293, B16-BL6, and CHO-K1 cells) that had these receptors inserted into them.[7] ... neuronal cell body. • neuron projection. • Q14325730. Biological process. • negative regulation of cell proliferation. • ... The density of GPR54 is not discernable in pyramidal cells, but has high levels of expression in the granule cell layer. It is ... In a study where malignant tumor cells were injected into a model system, the system was then tested for genes involved in the ...
... and its glycoforms on the function of human thyrocytes and CHO cells transfected with the human TSH receptor". Molecular and ...
Cho RW, Clarke MF (February 2008). "Recent advances in cancer stem cells". Curr. Opin. Genet. Dev. 18 (1): 48-53. doi:10.1016/j ... இவை அந்தந்த உறுப்புகளில் நிலவும் சாதரண குருத்தணுக்களில் (normal stem cells, ex. neuronal stem cells) உள்ள கல குறிகைகளில் (cell ... மேலும் புற்று உயிரணுக்களில் மிக குறைந்த அளவுகளில் புற்று குருத்தணுக்களை (cancer stem cells, Glioblastoma stem cell) அண்மையில் ... doi:10.1016/j.cell.2006.05.051. பப்மெட் 16901782. *↑ Croce CM (January 2008). "Oncogenes and cancer". The New England journal ...
"A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells". Nucleic Acids ... Daughter cells that retain a copy of the plasmid survive, while a daughter cell that fails to inherit the plasmid dies or ... The cells after transformation are exposed to the selective media, and only cells containing the plasmid may survive. In this ... Many of the genes carried by a plasmid are beneficial for the host cells, for example: enabling the host cell to survive in an ...
5-HT2B and 5-HT2C receptors in CHO-K1 cells". British Journal of Pharmacology. 128 (1): 13-20. doi:10.1038/sj.bjp.0702751. PMC ... neural crest cell migration. • positive regulation of cell proliferation. • positive regulation of ERK1 and ERK2 cascade. • ... neural crest cell differentiation. • negative regulation of apoptotic process. • negative regulation of cell death. • ... cell junction. • neuron projection. • integral component of plasma membrane. • nucleoplasm. • dendrite. Biological process. • ...
Cho SH, Chen CH, Tsai FS, Godin JM, Lo YH (June 2010). "Human mammalian cell sorting using a highly integrated micro-fabricated ... Cell sorting is the process of taking cells from an organism and separating them according to their type. The cells are ... There are three major methods used for cell sorting: single cell sorting, fluorescent activated cell sorting, and magnetic ... "Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)". BMC Cell Biology. 18 (1): 30. doi:10.1186/ ...
Lee, Kwang Jae; Kim, Yeong Bae; Kim, Jang Hee; Kwon, Hoek Chun; Kim, Dong Kyu; Cho, Sung Won (2008). "The alteration of ... Enterochromaffin (EC) cells (also known as Kulchitsky cells) are a type of enteroendocrine cell, and neuroendocrine cell. They ... ECL cells histologically appear similar to EC cells and are hence named as such. They are however a different cell type and do ... EC cells are small polygonal cells located in the crypts between intestinal villi. They are discriminated from other cells of ...
"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ... "Stable expression of human H1-histamine-receptor cDNA in Chinese hamster ovary cells. Pharmacological characterisation of the ... "Identification of serotonin 5-HT3 recognition sites in membranes of N1E-115 neuroblastoma cells by radioligand binding" ...
"A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells". Nucleic Acids Res ... Lederberg J (1952). "Cell genetics and hereditary symbiosis". Physiol. Rev. 32 (4): 403-430. PMID 13003535. ... "Strategies for the episomal modification of cells". Current Opinion in Molecular Therapeutics 11 (4): 433-441. PMID 19649988 ... "pEPito: a significantly improved non-viral episomal expression vector for mammalian cells" (PDF). BMC Biotechnol 10: 433-441. ...
... a type 2 diabetic will have lost about half of their beta cells.[52] Fatty acids in the beta cells activate FOXO1, resulting in ... Cho Y, Nadeau-Fredette AC, Burke M, Faruque L, Lloyd A, Ahmad N, Liu Y, Tiv S, Wiebe N, Strippoli GF (July 2016). "Comparison ... Type 2 diabetes is due to insufficient insulin production from beta cells in the setting of insulin resistance.[13] Insulin ... In the early stages of insulin resistance, the mass of beta cells expands, increasing the output of insulin to compensate for ...
CHO, Z. H., ERIKSSON L., and CHAN J.K., ``A circular ring transverse axial positron camera in Reconstruction Tomography in ... This tracer is a glucose analog that is taken up by glucose-using cells and phosphorylated by hexokinase (whose mitochondrial ... Although many investigators took this approach, James Robertson[66] and Zang-Hee Cho[67] were the first to propose a ring ... This means that FDG is trapped in any cell that takes it up until it decays, since phosphorylated sugars, due to their ionic ...
Zhou T, Zhang J, Carter R, Kimberly R (2003). "BLyS and B cell autoimmunity.". Curr. Dir. Autoimmun. 6: 21-37. PMID 12408045. ... Wu Y, Bressette D, Carrell JA, Kaufman T, Feng P, Taylor K, Gan Y, Cho YH, Garcia AD, Gollatz E, Dimke D, LaFleur D, Migone TS ... Brink R (2007). "Regulation of B cell self-tolerance by BAFF.". Semin. Immunol. 18 (5): 276-83. PMID 16916609. doi:10.1016/j. ... Mackay F, Leung H (2007). "The role of the BAFF/APRIL system on T cell function.". Semin. Immunol. 18 (5): 284-9. PMID 16931039 ...
These M cells then alert the underlying B cells and T cells in the tonsil that a pathogen is present and an immune response is ... Wang, JH; Chung, YS; Cho, YW; Kim, DY; Yi, JS; Bae, JS; Shim, MJ (April 2010). "Palatine tonsil size in obese, overweight, and ... The tonsils have on their surface specialized antigen capture cells called M cells that allow for the uptake of antigens ... "Tonsils Make T-Cells, Too, Ohio State Study Shows". Ohio State University. Ohio State University, Comprehensive Cancer Center. ...
Cho WC, Nam OH, Kim MS, Lee HS, Choi SC (May 2018). "A retrospective study of traumatic dental injuries in primary dentition: ... as this will damage the delicate cells that make up the tooth's interior. ...
"cho" በሦስት መክተብ ኣያፈጥንም። ተጨማሪ ምሳሌ ከኣስፈለገ ኣንድ የኢትዮጵያ ኦርቶዶክስ ቤተ ክርስቲያን ዓማርኛ በላቲን ፊደል እንዲጻፍና በኣጻጻፉም በግዕዝ ፊደል ምትክ በላቲን ፊደል ጽፎ ያቀረበው ... 1,306] Cell Phone Typing in Amharic የእጅ ስልክ. *[1,307] የእጅ ስልክ. *[1,308] Ethiopian eResources ...
Cho DY, Frey RA, Guffy MM, Leipold HW (November 1975). "Hypervitaminosis A in the dog". American Journal of Veterinary Research ... Wu J, Zern MA (2000). "Hepatic stellate cells: a target for the treatment of liver fibrosis". Journal of Gastroenterology. 35 ( ... Vitamin K prevents hypoprothrombinemia in rats and can sometimes control the increase in plasma/cell ratios of vitamin A.[49] ... Levine PH, Delgado Y, Theise ND, West AB (February 2003). "Stellate-cell lipidosis in liver biopsy specimens. Recognition and ...
cell nucleus. • kinetochore. • centrosome. • rough endoplasmic reticulum. • dendritic shaft. • aggresome. • cell surface. • ... Cho S, Lu M, He X, Ee PL, Bhat U, Schneider E, Miele L, Beck WT (December 2011). "Notch1 regulates the expression of the ... cell cortex. • integral component of membrane. • azurophil granule membrane. • Z disc. • neuronal cell body. • perinuclear ... cell-cell adhesion. • cellular response to amyloid-beta. • negative regulation of core promoter binding. • negative regulation ...
Kim JE, Cho BK, Cho DH, Park HJ (July 2013). "Expression of hypothalamic-pituitary-adrenal axis in common skin diseases: ... There, CRH and vasopressin act synergistically to stimulate the secretion of stored ACTH from corticotrope cells. ACTH is ... in immune cells, such as monocytes and neutrophils [8][9][11][12] ...
Kim, Jin-Ho; Kim, Hyo-Sop; Lee, Jae-Hyeok; Choi, Sung-Wook; Cho, Yong-Jin; Kim, Jae-Ho (2009-12-01). "Hexagonally Close Packed ... to improve cell adhesion or study the properties of biofilms. An example of Langmuir-Blodgett troughs' utility in ...
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... Yi, Eun Hee; Lee, Chang Seok; Lee, Jin-Ku; Lee, Young Ju; Shin, Min Kyung; Cho, Chung-Hyun; Kang, Keon Wook; Lee, Jung Weon; ... These T cells can then go on to perform effector functions such as macrophage activation, B cell activation, and cell-mediated ... When interleukin-1 is produced in response to external stimuli, it can bind to cell-surface receptors on the same cell that ...
Cho-Vega JH, Tsavachidis S, Do KA, Nakagawa J, Medeiros LJ, McDonnell TJ (2007). "Dicarbonyl/L-xylulose reductase: a potential ... Cell. Mol. Life Sci. 66 (9): 1570-9. doi:10.1007/s00018-009-9065-y. PMID 19337691.. ...
Place cells are also found in the hippocampus. The parietal cortex encodes spatial information using an egocentric frame of ... Cho, Y. H.; Kesner, R. P. (1996). "Involvement of entorhinal cortex or parietal cortex in long-term spatial discrimination ... Brun, V. H.; Otnaess, M. K.; Molden, S.; Steffenach, H.; Witter, M. P.; Moser, M.; Moser, E. I. (2002). "Place cells and place ... Participants are presented with a series of matrix patterns that have half their cells coloured and the other half blank. The ...
Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY ( ... The orphan nuclear receptor ROR-γ directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126:1121- ... Aggarwal S, Ghilardi N, Xie MH, de Sauvage FJ, Gurney AL (2003). "Interleukin-23 promotes a distinct CD4 T cell activation ... "Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells". Proc. Natl. Acad. Sci. U.S.A. 103 (21): ...
PDF)You've got the power: the evolution of batteries and the future of fuel cells. Toshiba. Hentet 17. maj 2009.. ... Karki, Khim; Epstein, Eric; Cho, Jeong-Hyun; Jia, Zheng; Li, Teng; Picraux, S. Tom; Wang, Chunsheng; Cumings, John (2012-03-14 ... Battery Anodes , Batteries & Fuel Cells , Research , The Energy Materials Center at Cornell. Emc2.cornell.edu. Hentet 10. ...
Human embryonic stem cells also more readily differentiate into cortical stem cells in the presence of retinoids[8] ... Kafi, R; Kwak, HS; Schumacher, WE; Cho, S; Hanft, VN; Hamilton, TA; King, AL; Neal, JD; Varani, J; Fisher, GJ; Voorhees, JJ; ... Retinoids have many important functions throughout the body including roles in vision,[1] regulation of cell proliferation and ... Retinol enters the absorptive cells of the small intestine, preferentially in the all-trans-retinol form. ...
Meeyoung, Cho (4 August 2009). "South Korea unveils CO2 target plan". Reuters. Seoul. Retrieved 8 April 2010.. ... "UNEP and Daimler Call for Infrastructure for Electric and Fuel-cell Vehicles". Climate-L.org. 4 July 2008. Archived from the ...
CHO. {\displaystyle {{\ce {-CHO}}}}. ) ordezkatzen du eta kate bateko 2 lisinen eta beste kate paralelo bateko 2 lisinen ... a b c d e f g E., Sadava, David (1993) Cell biology : organelle structure and function Jones and Bartlett Publishers ISBN ... a b c d e f g (Ingelesez) «1416023887 - Cell Biology, Updated Edition: with Student Consult Online Access by Thomas D Pollard ... The Plant Cell Wall» Molecular Biology of the Cell. 4th edition . Noiz kontsultatua: 2018-12-08 . ...
cell cycle arrest. • learning or memory. • cellular copper ion homeostasis. • cellular response to copper ion. • cell cycle. • ... Hwang D, Lee IY, Yoo H, Gehlenborg N, Cho JH, Petritis B, Baxter D, Pitstick R, Young R, Spicer D, Price ND, Hohmann JG, ... cell surface. • endoplasmic reticulum. • membrane raft. • anchored component of membrane. • extracellular exosome. • cell ... PrP immune cells include hematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it ...
The final structure of the abscess is an abscess wall, or capsule, that is formed by the adjacent healthy cells in an attempt ... Barbic, D; Chenkin, J; Cho, DD; Jelic, T; Scheuermeyer, FX (10 January 2017). "In patients presenting to the emergency ... The cytokines trigger an inflammatory response, which draws large numbers of white blood cells to the area and increases the ... However, such encapsulation tends to prevent immune cells from attacking bacteria in the pus, or from reaching the causative ...
mast cell granule. • Schwann cell microvillus. • Schmidt-Lanterman incisure. • nucleoplasm. • cell projection cytoplasm. • ... Park J, Kim H, Park SY, Lim SW, Kim YS, Lee DH, Roh GS, Kim HJ, Kang SS, Cho GJ, Jeong BY, Kwon HM, Choi WS (May 2014). " ... The involvement in oxidative stress diseases, cell signal transduction and cell proliferation process endows AKR1B1 the ... cell signal transduction and cell proliferation process including cardiovascular disorders, sepsis, and cancer.[13] ...
Islam MA, Yun CH, Choi YJ, Cho CS (2010). "Microencapsulation of live probiotic bacteria" (PDF). Journal of Microbiology and ... as well as increasing the proportion of T lymphocytes and natural killer cells.[96][97] LAB products might aid in the treatment ... or frozen yogurt products that contain 10 million cells per gram at the time of manufacture.[47] In 2002, the FDA and WHO ... but most companies that give a number report the viable cell count at the date of manufacture, a number that could be much ...
Acharya, P. V., Goldman, D. S. Chemical composition of the cell wall of the H37Ra strain of Mycobacterium tuberculosis. Journal ... Ha, S., Jeon, B., Youn, J., Kim, S., Cho, S., Sung, Y. Protective effect of DNA vaccine during chemotherapy on reactivation and ... Herrmann, J., Lagrange, P. Dendritic cells and Mycobacterium tuberculosis: which is the Trojan horse?. Pathologie Biologie. ... Pai, M., Zwerling, A., Menzies, D. Systematic Review: T-Cell-Based Assays for the Diagnosis of Latent Tuberculosis Infection: ...
"In Wang, Yu-li; Discher, Dennis E. (eds.). Cell Mechanics. Methods in Cell Biology. 83. Elsevier Inc. pp. 473-493. ISBN 978-0- ... Min, Duyoung; Kim, Kipom; Hyeon, Changbong; Cho, Yong Hoon; Shin, Yeon-Kyun; Yoon, Tae-Young (2013-04-16). "Mechanical ... "The Journal of Cell Biology. 101 (1): 130-140. doi:10.1083/jcb.101.1.130. ISSN 0021-9525. PMC 2113644. PMID 4040136.. ... in whole cells. The phagocytosis method previously described is useful for capturing a magnetic bead inside a cell. Measuring ...
Kang JS, Cho D, Kim YI, Hahm E, Kim YS, Jin SN, Kim HN, Kim D, Hur D, Park H, Hwang YI, Lee WJ. . (July 2005). "Sodium ... Effect of sodium ascorbate on the chemiluminescent response of murine peritoneal exudate cells". Life Sciences 50 (11): 753-759 ... "Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to ... ascorbate (vitamin C) induces apoptosis in melanoma cells via the down-regulation of transferrin receptor dependent iron uptake ...
Lee SW, Cho BH, Park SG, Kim S (August 2004). "Aminoacyl-tRNA synthetase complexes: beyond translation". Journal of Cell ... Molecular Cell Biology. 11 (9): 668-74. doi:10.1038/nrm2956. PMC 3042954. PMID 20700144.. ... and when released exert homeostatic and developmental control in specific human cell types, tissues and organs during adult or ... these proteins control gene expression within the cell of origin, ...
From analyzing CHO cell lines, researchers found that the transcriptome of each subclone also had a significant number of ... individual changes and that such changes indicate that epigenetic regulation is a hidden but important player in cell line ... Phenotypic variations in the cells arise from changes in their DNA, including differences in methylation patterns. ... cells for early studies. To produce a CHO cell line that can be used in production, scientists use single-cell cloning. " ...
reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. ... The host cell line (CHOZN® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. Mild ... reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1 ... Overexpressing Mgat1 in wildtype CHO cells did not lead to increased sialylation ...
... cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day- ... posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) ... Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i dont ... Have you done a viability assay to ensure that the floating cells are, in fact, dead?. CHO cells are damned hardy and will ...
Gently resuspend cell pellet in 2 ml freezing medium*.. * Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, ... Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. ... Freezing CHO Cells. Christina McGee 4-5-2012. * Grow 10 cm dish ... Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO ( ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Sack:_Freezing_CHO_cells&oldid=667947" ...
i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ... posted in Tissue and Cell Culture: Hi all, ... cho cells as that may change some properties of the cho cells. ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ...
MC2R-CRE-bla-CHO-K1CellsTheGeneBLAzer®MC2R-CRE-bla-CHO-K1cellscontainthehumanmelanocortin-2receptor(MC2R),(Accession#NM_ ... 规格: 2 × 10^6 cells. 名称: GeneBLAzer® MC2R-CRE-bla-CHO-K1 Cells. The GeneBLAzer® MC2R-CRE-bla-CHO-K1 cells contain the human ... CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat # K1129) contain a beta-lactamase (bla) reporter gene under ... o GeneBLAzer ® MC2R-CRE-bla-CHO-K1 cells. o GeneBLAzer ® MC2R-CRE -bla CHO-K1 Assay Protocol. o Certificate of Analysis. The ...
CHO) K1 cell line. Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its ... "We are extremely encouraged by the adoption of our GS knockout CHO K1 cell line by Glenmark. This proprietary solution has now ... The system includes the GS knockout CHO K1 cell line, a comprehensive package of supporting documentation, and an expression ... Horizon Discovery signs license agreement with Glenmark Pharmaceuticals for GS knockout CHO cell line for manufacture of ...
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a ... Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and ...
This bundled product includes one liter of Irvine Scientific BalanCD CHO Growth A medium and a CELLine 350 Bi ... CHO Growth A Medium and one CELLine 350 Bioreactor flask for a multiple harvest production run ,p, ... CELLine 350 / 1L Media CHO A. Write Your Own Review. How do you rate this product? *. 1 star. 2 stars. 3 stars. 4 stars. 5 ... This will allow for multiple harvests from the 5ml cell compartment. The 5ml cell compartment can sustain over 100 million ...
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin ... The motilin pharmacophore in CHO cells expressing the human motilin receptor Biochem Biophys Res Commun. 2002 May 17;293(4): ... N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin ...
The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ... Recombinant Protein Therapeutics from CHO Cells -- 20 Years and Counting. The CHO cell is at its height of technological ... This is a major driver in further understanding Chinese Hamster Ovary (CHO) cell biology, which is the cell line of choice for ... The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ...
PrecisION Ion Channel Cell Lines ,High Quality, Functionally-Validated, Ion Channel Cell Lines ,,Ion channels are well known ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate, ... hERG-CHO K1 Recombinant Cell Line from Upstate. 7. hERG-CHO K1 ... hHCN4-CHO K1 Recombinant Cell Line from Upstate. 9. hKv1.5-CHO K1 Recombinant Cell Line from Upstate. 10. hNav1.6-HEK293 ... hKv4.2/hKChIP2-CHO K1 Recombinant Cell Line from Upstate. 6. ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate. ...
EXCELL Advanced CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ... CHO Feed 1 for superior platform performance in fed-batch cultures on all industrial CHO cell lineages (CHO-S, DuxB11, DG44, ... Determine the correct volume of cell culture to inoculate a new flask at a starting cell density of 2-3 x105 cells/ml in a ... EX-CELL® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ...
PrecisION Ion Channel Cell Lines ,High Quality, Functionally-Validated, Ion Channel Cell Lines ,,Ion channels are well known ... hKv4.2/hKChIP2-CHO K1 Recombinant Cell Line from Upstate, ... hERG-CHO K1 Recombinant Cell Line from Upstate. 6. hERG-CHO K1 ... hHCN4-CHO K1 Recombinant Cell Line from Upstate. 8. hKv1.5-CHO K1 Recombinant Cell Line from Upstate. 9. hNav1.6-HEK293 ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate. 11. Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem. ...
A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros ... Chinese Hamster Ovary Cells (CHO-K1 Line). A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of ...
Importantly, in cell-cell adhesion assays between CD2+ Jurkat T cells and CD48- or CD59-transfected CHO cells, there was no ... Gene context of CHO Cells. *Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3(-/-) chicken DT40 cells, indicating ... cells [15].. *Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the ... The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell ...
... we have transfected each of these genes into Chinese hamster ovary cells (CHO-K1) and have established stable cell lines ... Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells.. Buckley NJ1, Bonner TI, Buckley ... National Institute of Mental Health, Laboratory of Cell Biology, Bethesda, Maryland 20892.. ...
Read the StainFree analysis of CHO cells using SoftMax Pro Software. ... CHO cells are an epithelial-like cell line commonly used in biological and medical research. ... CHO cells in these images were plated at 4000 cells per well in a 384-well microplate. Left: To create a new StainFree analysis ... To count CHO cells without staining, I use the predefined setting Cells A in SoftMax Pro Software. This works really well on ...
Cell. 1996 Nov 1;87(3):415-26. Comparative Study; Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S. ... Cell. 1996 Nov 1;87(3):415-26.. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein.. ... CHO 25-RA (CVCL_U429) - Cellosaurus - a cell line knowledge resource. Miscellaneous. *CHOLESTEROL - Hazardous Substances Data ... The cDNA was isolated from Chinese hamster ovary cells with a dominant mutation that renders them resistant to sterol-mediated ...
CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. Transport of ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... defective compared to vesicles from wild-type cells, whereas transport of other nucleotide sugars was normal. These cells do ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ...
CHO glycosylation mutants. CHO-Lec1 cells completely lack complex and hybrid-type N-glycans on glycoproteins.. ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ...
Bjørn Voldborg, MSc, Director, CHO Cell Line Development, The Novo Nordisk Foundation Center for Biosustainability ... and since 2012 Bjørn has been heading the CHO Cell Line Engineering project at the NNF Center for Biosustainability at the ... Bjørn Voldborg of the Technical University of Denmark joins us to discuss the impact of big data on cell line engineering, his ... Production of Hard-to-Produce Proteins with CHO. ... to the engineering of improved protein production cell ...
Dowd, J. E., Kwok, K. E. and Piret, J. M. (2001), Glucose-based optimization of CHO-cell perfusion cultures. Biotechnol. Bioeng ...
727e) A Model-Based Strategy for Understanding and Improving Recombinant Protein Glycosylation in CHO Cells. ... Our platform has been validated against experimental data from two IgG-producing CHO cell lines representing all aforementioned ... Specifically, the higher scale model is a cell culture dynamics (CCDyn) model, which describes cell growth and the variation of ... CHO) cells, the workhorse of the biopharmaceutical industry. Our platform constitutes a multi-scale model describing the ...
Catalog Products » Other Products » Stable Cell Lines » Human Recombinant NK2 Tachykinin Receptor Stable Cell Line ... Human Recombinant NK2 Tachykinin Receptor Stable Cell Line Description. Tachykinins are peptides sharing a common C-terminal ...
GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. ... GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays.. ... Catalog Products » Other Products » Stable Cell Lines » Human Recombinant BB2 Bombesin Receptor Stable Cell Line ... Human Recombinant BB2 Bombesin Receptor Stable Cell Line Description. The bombesin receptor family is a member of the G protein ...
... cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration ... Hiller GW, Ovalle AM, Gagnon MP, Curran ML, Wang WG (2017) Cell-controlled hybrid perfusion fed-batch CHO cell process provides ... CHO Site-specific integration CRISPR/Cas9 Cell line development C12orf35 Electronic supplementary material. The online version ... Li S, Gao X, Peng R, Zhang S, Fu W, Zou F (2016) FISH-based analysis of clonally derived CHO cell populations reveals high ...
CHO Stable Cell Line Development in Science/R&D with NGM Biopharmaceuticals, Inc.. Apply Today. ... Broad experience in cell biology, including mammalian cell culture, cell transfection, and cell-based functional assays ... Develop stable cell lines including clone screening, single cell cloning, and fed-batch production ... Evaluate the CHO CLD and production process with varies media. *Design studies and interpret results of development, ...
... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells in DOAJ. DOAJ is an online ... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells. BMC Biotechnology. 2009;9(1):76 DOI ... It is shown that the manipulation of cell cycle kinetics and indirectly cell metabolism gives higher cell densities in CHO ... gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the ...
In AT1A receptor transfected CHO-K1 cells, angiotensin II (10−9 M) stimulated a rapid increase in cytosolic free calcium that ... Stable expression of a functional rat angiotensin II (AT1A) receptor in CHO-K1 cells: Rapid desensitization by angiotensin II. ... To address these problems, we expressed the recombinant AT1A receptor in CHO-K1 cells. The stably transfected receptor was ... high-level transfectant of the AT1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following ...
Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine ... Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine ... This also provide an explanation for the observed thermo-sensitive phenotype of CHO-MT58 cell line. ... The CHO-MT58 cell line expresses a mutant variant of CCT, and displays a thermo-sensitive phenotype. At non-permissive ...
... but the only vendor I see refer to their cells as DG44 is at Invitrogen. All other cell line vendors just refer to them as CHO ... posted in Cell Biology: I am looking to purchase DG44 cells because these are the cells that I see appear in the literature ... I know that DG44 cells are dhfr- mutants, ... The Differences Between CHO DG44 and CHO dhfr- - ... All other cell line vendors just refer to them as CHO dhfr-. Is there an actual difference do you think between cells ...
Keywords: Animals ; Bioreactors ; CHO Cells/*physiology ; Cell Culture Techniques/methods ; Cell Proliferation ; Cell Survival ... CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear ... Serum-free large-scale transient transfection of CHO cells. Derouazi, M.; Girard, P.; Van Tilborgh, F.; Iglesias, K.; Muller, N ... The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected ...
... simple luminescent calcium flux assays using irradiated AequoScreen cells tranfected with human GPR99 receptor - no cell ... Human Recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells ... The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we ... Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you ...
div class=section, ,h2,Reduce the Time Needed to Isolate Producing, Monoclonal Cell Lines,/h2, ,h3,Background,/h3, ,p,The ... ClonaCell™-CHO methylcellulose-based semi-solid media are specifically designed for cloning of CHO cell lines. ClonaCell™-CHO ... Perform cell transfection.. *Incubate the cells in liquid recovery medium (such as ClonaCell™-CHO CD Liquid, Catalog #03817) ... Using the standard protocol for mammalian cell cloning in ClonaCell™-CHO media, freshly transfected cells are suspended in ...
  • Horizon Discovery Group plc (LSE: HZD) ("Horizon"), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase ("GS") knockout Chinese Hamster Ovary (CHO) K1 cell line. (horizondiscovery.com)
  • We are extremely encouraged by the adoption of our GS knockout CHO K1 cell line by Glenmark. (horizondiscovery.com)
  • The system includes the GS knockout CHO K1 cell line, a comprehensive package of supporting documentation, and an expression vector supplied under license from DNATwoPointO, Inc.. This biomanufacturing platform allows these companies to move from the DNA sequence of their potential biotherapeutic to clinical manufacturing as simply and rapidly as possible. (horizondiscovery.com)
  • To produce a CHO cell line that can be used in production, scientists use single-cell cloning. (genengnews.com)
  • From analyzing CHO cell lines, Weinguny and his colleagues found that "the transcriptome of each subclone also had a significant number of individual changes," and the scientists pointed out that such changes "indicate that epigenetic regulation is a hidden, but important player in cell line development with a major role in the establishment of high performing clones with improved characteristics for bioprocessing. (genengnews.com)
  • A functional hamster MGAT1 was overexpressed in an Mgat1-disrupted IgG producing cell line (Mgat1 KO37). (sigmaaldrich.com)
  • The host cell line (CHOZN ® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. (sigmaaldrich.com)
  • Mgat1KO37OE4 expresses Mgat4b significantly higher, while OE12's Mgat4b is significantly lower, than the non-ZFN transfected cell line. (sigmaaldrich.com)
  • Mgat1KO37 has significantly decreased Mgat4b than non-ZFN transfected cell line where it was derived from. (sigmaaldrich.com)
  • The host cell line that does not express IgG has significantly higher Mgat4b than the IgG-expressing non-ZFN transfected line. (sigmaaldrich.com)
  • Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, date and your initials. (openwetware.org)
  • Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Company's biomanufacturing processes. (horizondiscovery.com)
  • These licenses have been taken as a result of an unmatched combination of high performance, transparent cell line history, supporting documentation, and attractive licensing terms. (horizondiscovery.com)
  • Hypoglycaemia attenuates cyanide-mediated transcription in non-transformed HIF-1 wild-type CHO cells but not the human tumour derived cell line. (ox.ac.uk)
  • We demonstrate that manipulation of redox in air, achieved by inhibiting cytochrome oxidase with cyanide, induces HIF-1 mediated transcription in wild-type CHO and HT1080 human tumour cells but not in CHO cells deficient in the oxygen responsive, HIF-1alpha sub-unit of HIF-1. (ox.ac.uk)
  • Horizon licenses its CHO expression system to pharmaceutical, biotechnology, and biosimilar companies, as well as contract manufacturing organizations. (horizondiscovery.com)
  • Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO (dimetylsulfoxide, sterile). (openwetware.org)
  • reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. (sigmaaldrich.com)
  • In support of this we demonstrate that HIF-1alpha deficient cells are less tumorigenic than wild-type cells. (ox.ac.uk)
  • Built upon more than a decade of experience in the engineering of cell lines, Horizon offers an unmatched portfolio of tools and services to help scientists gain a greater understanding of gene function, identify genetic drivers behind human disease, deliver biotherapeutics, cellular and gene therapies for precision medicine as well as develop and validate diagnostic workflows. (horizondiscovery.com)
  • Horizon's solutions enable almost any gene to be altered, or its function modulated, in human and other mammalian cell lines. (horizondiscovery.com)
  • Technologies and methods that result in the development and identification of robust and stable CHO pools and clones that can be paired with platform media, feed, and bioprocesses will be imperative for reducing timelines," says Oren Beske, amalgamator of business and biology, ATUM. (genengnews.com)
  • Overall, he points out that biopharma needs "CHO pools and clones that can rapidly be scaled up to the appropriate scale without process development and with a low-risk profile. (genengnews.com)
  • Other experts agree on the value of reliable CHO cell lines. (genengnews.com)
  • Given the wide use of CHO cells, reliable and vigorous lines are exactly what researchers and drug developers need. (genengnews.com)
  • Zinc-finger nuclease (ZFN) technology was used to create our Mgat1-disrupted CHO lines with high-mannose dominant simple N-glycoforms. (sigmaaldrich.com)
  • The results support the approach of Mgat1 and Mgat4 co-overexpression to create host cell lines for biopharmaceutical production with increased sialylation. (sigmaaldrich.com)
  • Incorporating this technology into our biomanufacturing processes enhances our ability to efficiently generate high quality cell lines. (horizondiscovery.com)
  • In developing biological drugs, scientists often rely on Chinese hamster ovary (CHO) cells for early studies. (genengnews.com)
  • Life Technologies Pledges $20M to Expand Cell Culture Capacity at Scottish. (genengnews.com)
  • Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. (umassmed.edu)
  • Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. (openwetware.org)
  • These cells should be robust such that a relatively generic platform process, such as one for monoclonal antibodies, could be applied with very little or no process development to rapidly create bulk drug substance for Phase I clinical trials," Beske says. (genengnews.com)
  • Cells were harvested for RNA isolation from Day 4, and for total cellular protein extraction from Day 7. (sigmaaldrich.com)
  • As Weinguny notes, the cells "display a wide array of observed phenotypes. (genengnews.com)
  • Human Recombinant, in CHO-K1 host cell. (perkinelmer.com)
  • Clincke M-F, Guedon E, Yen FT, Ogier V, Goergen J-L (2011) Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes. (springer.com)
  • Frozen Cells validated for cAMP testing: Serotonin 5-HT1A receptor, human recombinant, in CHO-K1 host cell. (perkinelmer.co.uk)
  • These cells should be robust such that a relatively generic platform process, such as one for monoclonal antibodies, could be applied with very little or no process development to rapidly create bulk drug substance for Phase I clinical trials," Beske says. (genengnews.com)
  • Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i don't know how to optimize the conditions in this aspect. (protocol-online.org)
  • i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. (protocol-online.org)
  • It is critical that the cumulative volume of the cell suspension added in this step and reagents added in step 5 does not exceed 10 mL. (stemcell.com)
  • Add the cell suspension in liquid growth medium to the semi-solid medium. (stemcell.com)
  • GE HyClone ActiPro medium and Cell Boost 7a and 7b supplements are designed and optimised for large-scale protein production in fed-batch suspension culture using recombinant CHO cells. (labonline.com.au)
  • In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. (selectscience.net)
  • Cellvento® CHO media have been developed for use with CHO suspension cell types such as CHO-S, CHO-DHFR- and CHO-K1 cells, but may also be suitable for other cell lines. (merckmillipore.com)
  • Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. (diva-portal.org)
  • In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. (diva-portal.org)
  • Here we describe the application of a closed-loop control scheme for the long-term cultivation of CHO cells in a high cell density (35 - 40 million cells/ml) perfusion process. (bioprocessintl.com)
  • Available in powder and compacted forms, Cellvento® CHO formulations are chemically defined, of non-animal origin and lead to superior cell growth and productivity in batch, fed-batch mode or perfusion applications. (merckmillipore.com)
  • EX-CELL ® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. (sigmaaldrich.com)
  • EX-CELL ® Advanced™ CHO Fed-Batch Medium is formulated without L-glutamine and without hypoxanthine/thymidine. (sigmaaldrich.com)
  • Transfer the entire contents aseptically into a 125-mL shake flask containing 30 mL prewarmed complete EX-CELL ® Advanced TM CHO Fed-Batch Medium. (sigmaaldrich.com)
  • Pre-warm complete EX-CELL ® Advanced™ CHO Fed-Batch Medium to room temperature. (sigmaaldrich.com)
  • Determine the correct volume of cell culture to inoculate a new flask at a starting cell density of 2-3 x10 5 cells/ml in a total volume of 30 mL fresh EX-CELL ® Advanced™ CHO Fed-Batch Medium per 125-mL shake flask. (sigmaaldrich.com)
  • Prepare a freezing medium consisting of 46.5% cold EX-CELL ® Advanced™ CHO Fed-Batch Medium, 46.5% conditioned medium and 7% dimethyl sulfoxide (DMSO). (sigmaaldrich.com)
  • ActiPro medium and Cell Boost 7a and 7b supplements can be used together in a fed-batch process or the medium and supplements can be used in combination with other products to increase productivity of an existing process. (labonline.com.au)
  • Ma N, Ellet J, Okediadi C, Hermes P, McCormick E, Casnocha S. A single nutrient feed supports both chemically defined NS0 and CHO fed-batch processes: improved productivity and lactate metabolism. (springer.com)
  • Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture. (springer.com)
  • Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. (semanticscholar.org)
  • Cellvento® CHO media and companion feeds support consistent growth and performance in fed-batch culture. (merckmillipore.com)
  • It is a high concentrated feed that comes in compacted format and is compatible with all Cellvento® CHO fed-batch media. (merckmillipore.com)
  • The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. (nexcelom.com)
  • Individual cells grow to form discrete, monoclonal colonies that are picked from the semi-solid medium after 10 - 14 days of incubation. (stemcell.com)
  • In our last situation, a major biopharmaceutical manufacturer incorporates CHO cell expression for monoclonal antibody production and purification, with filtration playing a major role in the production process. (thefreedictionary.com)
  • Dionne B, Mishra N, Butler M (2017) A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture. (springer.com)
  • Also, cultured cells show diminished angiotensin II receptor binding with respect to time in culture and passage number. (springer.com)
  • To address these problems, we expressed the recombinant AT 1A receptor in CHO-K1 cells. (springer.com)
  • In AT 1A receptor transfected CHO-K1 cells, angiotensin II (10 −9 M) stimulated a rapid increase in cytosolic free calcium that was completely desensitized within 50 sec following receptor occupancy. (springer.com)
  • Receptor desensitization was also unaffected by inhibition or activation of protein kinase C. Thus, we have established a permanent, high-level transfectant of the AT 1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following exposure to physiological concentrations of agonist. (springer.com)
  • Targeted disruption of CD22 in mice results in a reduced level of surface IgM on peripheral B cells, suggesting a role for CD22 in limiting antigen receptor signaling. (biovendor.com)
  • Anthrax protective antigen interacts with a specific receptor on the surface of CHO-K1 cells. (asm.org)
  • Pretreatment of cells with a number of different proteases strongly inhibits PA binding, suggesting that the receptor may be at least partially proteinaceous. (asm.org)
  • Our results suggest that a cell surface protein(s) of 85 to 90 kDa is, or constitutes a portion of, a specific receptor for the PA. (asm.org)
  • In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme. (perkinelmer.co.uk)
  • To confirm that the SPD-induced apoptotic cell death was due to the involvement of caspase-7, cells were also treated with SPD in the presence of the specific inhibitor of caspase-7, Ac-DEVD-CHO [34]. (nih.gov)
  • I use Freestyle CHO media from Gibco, shake flasks at ~120rpm. (protocol-online.org)
  • Here, the proof-of-concept was quite positive and showed an increase in cell growth for the FreeStyle CHO medium. (bioprocessintl.com)
  • Elucidating the role of copper in CHO cell energy metabolism using (13) C metabolic flux analysis. (springer.com)
  • Cell density and protein production were shown to be comparable between scales. (labonline.com.au)
  • CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. (thermofisher.com)
  • Therefore, this stimulated the interest of our research group to manipulate these miRNAs in CHO cells with a view to positively impact bioprocess-relevant CHO cell phenotypes. (dcu.ie)
  • The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. (rupress.org)
  • Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25. (rupress.org)
  • Gao Y et al (2016) Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess. (springer.com)
  • In the second approach, we targeted a previously verified miRNA for improved CHO cell growth and productivity i.e. miR-7, using a bacterial genome-editing tool, CRISPR-Cas9. (dcu.ie)
  • Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. (semanticscholar.org)
  • HyCell™ CHO establishes a new benchmark for cell growth and productivity. (technologynetworks.com)
  • Its versatility allows quick adaptation and supports exceptional growth, high cell density and high productivity. (technologynetworks.com)
  • Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. (perkinelmer.com)
  • FroZen Cells validated for calcium testing: GPR99. (perkinelmer.com)
  • Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. (perkinelmer.com)
  • E-1/E-2 failed to increase intracellular calcium in CHOdelta, CHOkappa and SH-SY5Y cells. (le.ac.uk)
  • Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. (perkinelmer.co.uk)
  • Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. (perkinelmer.co.uk)
  • PerkinElmer - ES-542-M400UA - Membrane Target Systems: Opioid Mu (OP3) (human) membrane preparation, in CHO-K1 cells. (neobits.com)
  • In single adherent cells E-1/E-2 (1 microM) increased [Ca2+]i with a mean 340/380 ratio change of 0.81+/-0.09 and 0.40+/-0.08 ratio units respectively. (le.ac.uk)
  • In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers. (frontiersin.org)
  • These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers. (frontiersin.org)
  • 70% increase in maximal cell density without additional supply of nutrients the cells underwent an overall reduction of 14% in size as well as a significant decrease in glucose and amino acid consumption rate. (doaj.org)
  • Cell density could be significantly further increased showing the capacity of the system set-up. (diva-portal.org)
  • These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance. (perkinelmer.com)
  • PerkinElmer's validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. (perkinelmer.com)
  • Here we analyze the functional properties of human beta 1B by expressing it in hamster CHO cells. (rupress.org)
  • The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. (scielo.br)
  • The aim of the present work was, therefore, to determine the effect of crotalid venom on CHO-K1 cells, particularly its action on actin filaments, endoplasmic reticulum and nucleus. (scielo.br)
  • Resuspend the cells in liquid growth medium (such as ClonaCell™-CHO CD Liquid, Catalog #03817). (stemcell.com)
  • These data highlight some of our results in applying RNAi to augment CHO cell growth by targeting both cell death and metabolic pathways that are detrimental to the optimal performance of cells grown in bioreactors. (thefreedictionary.com)
  • Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. (creativebiomart.net)
  • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. (creativebiomart.net)
  • What is the market size in 2018 and the growth rate of the Global Recombinant Hamster Ovary Cell (CHO) Hepatitis B Vaccine Market? (pharmiweb.com)
  • CHOMACS CD medium is a complete chemically-defined, animal component-free, protein-free, and ready-to-use media developed for long term, high performance growth and protein yield of CHO cells. (miltenyibiotec.com)
  • The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. (innoget.com)
  • In the initial assay without S9-mix, cell growth was essentially uneffected at Granola 97 concentrations up to and including 500 g/mL, growth was reduced to 72% compared to the solvent control at 1500 g/mL and reduced to 0% at 5000 g/mL. (epa.gov)
  • In the presence of S9-mix, cell growth was 55% of the solvent control at 1500 g/mL and 0% at 5000 g/mL. (epa.gov)
  • In the repeat assay after 20 hours exposure to Granola 97 without S9-mix, cell growth remained above 50% of the solvent control value at all concentrations except 3000 g/mL where growth was 4% of the solvent control. (epa.gov)
  • After 44 hours exposure, cell growth remained above 50% of the solvent control value at concentrations up to and including 500 g/mL and was reduced to 42%, 18% and 0% at 1000, 1500 and 3000 g/mL, respectively. (epa.gov)
  • In the presence of S9-mix (6 hour exposure), cell growth remained above 50% of the solvent control value at all concentrations except 3000 g/mL where growth was 3% of the solvent control. (epa.gov)
  • Our Membrane Target System membranes are prepared from cells that express recombinant or endogenous receptors. (neobits.com)
  • Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. (neobits.com)
  • 2 E-1 displaced [3H]-diprenorphine ([3H]-DPN) binding in CHO micro and SH-SY5Y membranes with pKi values of 8.02+/-0.09 and 8.54+/-0.13 respectively. (le.ac.uk)