CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Cricetulus: A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Ovary: The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Kinetics: The rate dynamics in chemical or physical systems.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Radioligand Assay: Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Adenine Phosphoribosyltransferase: An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Choline: A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.Diprenorphine: A narcotic antagonist similar in action to NALOXONE. It is used to remobilize animals after ETORPHINE neuroleptanalgesia and is considered a specific antagonist to etorphine.Cell Adhesion: Adherence of cells to surfaces or to other cells.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Tetrahydrofolate Dehydrogenase: An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3.Creatine: An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.PolysaccharidesMembrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Cynanchum: A plant genus of the family ASCLEPIADACEAE. Members contain steroidal glycosides and cytotoxic phenanthroindolizidine N-oxide alkaloids.Pregnenes: Unsaturated derivatives of PREGNANES.Biopharmaceutics: The study of the physical and chemical properties of a drug and its dosage form as related to the onset, duration, and intensity of its action.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Protein Engineering: Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Child Nutrition Sciences: The study of NUTRITION PROCESSES as well as the components of food, their actions, interaction, and balance in relation to health and disease of children, infants or adolescents.Child Day Care Centers: Facilities which provide care for pre-school and school-age children.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Thermodilution: Measurement of blood flow based on induction at one point of the circulation of a known change in the intravascular heat content of flowing blood and detection of the resultant change in temperature at a point downstream.Genetic Research: Research into the cause, transmission, amelioration, elimination, or enhancement of inherited disorders and traits.Cell Biology: The study of the structure, behavior, growth, reproduction, and pathology of cells; and the function and chemistry of cellular components.Research Subjects: Persons who are enrolled in research studies or who are otherwise the subjects of research.Health Status: The level of health of the individual, group, or population as subjectively assessed by the individual or by more objective measures.Polyethyleneimine: Strongly cationic polymer that binds to certain proteins; used as a marker in immunology, to precipitate and purify enzymes and lipids. Synonyms: aziridine polymer; Epamine; Epomine; ethylenimine polymer; Montrek; PEI; Polymin(e).Calcium Phosphates: Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

In this study the direct involvement of cadherins in adhesion-mediated growth inhibition was investigated. It is shown here that overexpression of N-cadherin in CHO cells significantly suppresses their growth rate. Interaction of these cells and two additional fibroblastic lines with synthetic beads coated with N-cadherin ligands (recombinant N-cadherin ectodomain or specific antibodies) leads to growth arrest at the G1 phase of the cell cycle. The cadherin-reactive beads inhibit the entry into S phase and the reduction in the levels of cyclin-dependent kinase (cdk) inhibitors p21 and p27, following serum-stimulation of starved cells. In exponentially growing cells these beads induce G1 arrest accompanied by elevation in p27 only. We propose that cadherin-mediated signaling is involved in contact inhibition of growth by inducing cell cycle arrest at the G1 phase and elevation of p27 levels.  (+info)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

The DNA repair protein apurinic endonuclease (APE/Ref-1) exerts several physiological functions such as cleavage of apurinic/apyrimidinic sites and redox regulation of the transcription factor AP-1, whose activation is part of the cellular response to DNA damaging treatments. Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towards AP-1, thus promoting DNA binding activity of AP-1. Inhibition of CKII mediated phosphorylation of APE/Ref-1 blocked mutagen-stimulated increase in AP-1 binding. It also abrogated the induction of c-Jun protein and rendered cells more sensitive to induced DNA damage. Thus, phosphorylation of APE/Ref-1 appears to be involved in regulating the different physiological activities of the enzyme. CKII mediated phosphorylation of APE/Ref-1 and concomitant increase in AP-1 binding activity appears to be a novel mechanism of cellular stress response, forcing transcription of AP-1 target gene(s) the product(s) of which may exert protective function.  (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located.  (+info)

Proteoglycan involvement in polyamine uptake. (4/15188)

We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake considerably, whereas chondroitin sulphate lyase had a limited effect. Inhibition of polyamine biosynthesis with alpha-difluoromethylornithine resulted in an increase of cell-associated heparan sulphate proteoglycans exhibiting higher affinity for spermine. The data indicate a specific role for heparan sulphate proteoglycans in the uptake of spermine by fibroblasts. Spermine uptake by pgsD-677, a mutant Chinese hamster ovary cell defective in heparan sulphate biosynthesis, was only moderately reduced (20%) compared with wild-type cells. Treatment of mutant cells with the above-mentioned xyloside resulted in a greater reduction of endogenous proteoglycan production as well as a higher inhibition of spermine uptake than in wild-type cells. Moreover, treatment with chondroitin sulphate lyase resulted in a selective inhibition of uptake in mutant cells, indicating a role for chondroitin/dermatan sulphate proteoglycans in the uptake of spermine by these cells. Fibroblasts, made growth-dependent on exogenous spermine by alpha-difluoromethylornithine treatment, were growth-inhibited by heparan sulphate or beta-D-xyloside, which might have future therapeutical implications.  (+info)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Serum-free culture filtrates of six Candida species and Saccharomyces cerevisiae were found to contain chemoattractants for human polymorphonuclear leukocytes (PMNs) and a mouse macrophage-like cell line, J774. The chemotactic factors differed for the PMN and J774 cells, however, in terms of heat stability, kinetics of liberation by the yeast cells, and divalent cation requirements for production. The chemoattractant in Candida albicans culture filtrates appeared to act through the formyl peptide receptor (FPR) of PMNs, since it was found to induce chemotaxis of Chinese hamster ovary (CHO) cells that were expressing the human FPR but did not induce chemotaxis of wild-type CHO cells. The C. albicans culture filtrates also induced migration of PMNs across confluent monolayers of a human gastrointestinal epithelial cell line, T84; migration occurred in the basolateral-to-apical direction but not the reverse direction, unless the epithelial tight junctions were disrupted. J774 cells did not migrate toward the formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albicans culture filtrate was not inhibited by an FPR antagonist (t-butoxycarbonyl-Met-Leu-Phe), suggesting that a different receptor mediated J774 cell chemotaxis. In conclusion, we have identified a receptor by which a non-serum-dependent chemotactic factor (NSCF) produced by C. albicans induced chemotaxis of PMNs. Additionally, we have shown that NSCF was active across epithelial monolayers. These findings suggest that NSCFs produced by C. albicans and other yeast species may influence host-pathogen interactions at the gastrointestinal tract mucosal surface by inducing phagocytic-cell infiltration.  (+info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology.  (+info)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen.  (+info)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein.  (+info)

*Dihydrofolate reductase

CHO cells[edit]. DHFR lacking CHO cells are the most commonly used cell line for the production of recombinant proteins. These ... which are important for cell proliferation and cell growth.[14] DHFR plays a central role in the synthesis of nucleic acid ... regulation of transcription involved in G1/S transition of mitotic cell cycle. • tetrahydrofolate metabolic process. • ... DHFR is responsible for the levels of tetrahydrofolate in a cell, and the inhibition of DHFR can limit the growth and ...

*Morwa indyjska, wolna encyklopedia

The clastogen-suppressing effects of Tochu tea in CHO cells and mice. „Mutation research". 388 (1), s. 7-20, 1997. DOI: 10.1016 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line.. „Cancer Research". 61 (15), s. 5749 ... Scopoletin suppresses pro-inflammatory cytokines and PGE2 from LPS-stimulated cell line, RAW 264.7 cells.. „Fitoterapia". 75 (3 ... Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor kappaB and caspase-3 ...

*FANCG

"The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells". Proc Natl Acad Sci U S A. ... "Role of Fanconi DNA repair pathway in neural stem cell homeostasis". Cell Cycle. 7 (13): 1911-5. doi:10.4161/cc.7.13.6235. PMID ... Cell. 7 (2): 249-62. doi:10.1016/s1097-2765(01)00173-3. PMID 11239454. Yang Y, Kuang Y, Montes De Oca R, Hays T, Moreau L, Lu N ... Cell. Biol. 19 (7): 4866-73. doi:10.1128/mcb.19.7.4866. PMC 84285 . PMID 10373536. Park SJ, Ciccone SL, Beck BD, Hwang B, Freie ...

*Zygosity

"Evidence obtained by segregation analysis for functional hemizygosity at the Emtr locus in CHO cells". Cell. 14: 1007-1013. doi ... In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ... A chromosome in a diploid organism is hemizygous when only one copy is present.[2] The cell or organism is called a hemizygote ...

*Etrolizumab

... it is manufactured in CHO cells. As of 2016 it was in phase III studies for induction and maintenance therapy in people with ... Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ...

*Pertuzumab

It is manufactured recombinantly in CHO cells. The monoclonal antibody 2C4 appears to have first been published in 1990 by ... sets off signal transduction through several pathways that stimulate cell proliferation and cell growth; if overexpressed it ... Pertuzumab had also been studied in Non-small cell lung cancer but as of 2016 that indication had been discontinued. As of 2016 ... Badache, A; Hynes, NE (April 2004). "A new therapeutic antibody masks ErbB2 to its partners" (PDF). Cancer Cell. 5 (4): 299-301 ...

*Etoperidone

"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ...

*Imaging cycler microscopy

"3D all-organelle real time visualization of a single cell." "Visualizing the protein-DNA network code inside the cell nucleus ... Krusche, A (2013). "TIS robot" in Dubitzky, Wolkenhauer, Cho, Yokota. Encyclopedia of Systems Biology. Springer New York. pp. ... Detection of 2000 Cell Surface Protein Clusters in a Single Tissue Section and Cell Type Specific Annotation by Using a Three ... detection of CD8+ CD18+ cells and CD8+ CD103+ cells by multi-epitope imaging". Clinical and Experimental Immunology. 112 (1): ...

*Ro60-0175

5-HT2B and 5-HT2C receptors in CHO-K1 cells". British Journal of Pharmacology. 128 (1): 13-20. doi:10.1038/sj.bjp.0702751. PMC ...

*Pembrolizumab

It is recombinantly manufactured in Chinese hamster ovary (CHO) cells. Pembrolizumab was invented by scientists Gregory Carven ... It blocks a protective mechanism of cancer cells, and allows the immune system to destroy those cancer cells. It targets the ... metastatic non-small cell lung cancer (NSCLC) in certain situations, as a second-line treatment for head and neck squamous cell ... Results of a Phase II clinical trial in Merkel-cell carcinoma were reported in the New England Journal of Medicine in June 2016 ...

*Enzyme replacement therapy

... animal cells (i.e. Chinese hamster ovary cells, or CHO cells), and plant cells. Lysosomal storage diseases are fatal group of ... These cells have the ability to mature into the many cell types that comprise blood, including red blood cells, platelets, and ... Hematopoietic stem cell (HSC) transplantation is another treatment for lysosomal storage diseases. HSCs are derived from bone- ... Once inside the body the vector introduces the therapeutic gene into host cells, and the protein encoded by the newly inserted ...

*Zeocin

"Phleomycin resistance as a dominant selectable marker in CHO cells". Somatic Cell and Molecular Genetics. 14 (3): 243-52. doi: ... It causes cell death by intercalating into DNA and induces double strand breaks of the DNA. Zeocin is blue in colour due to the ... When zeocin enters a cell, the Cu2+ is reduced to Cu+ and then removed, and zeocin becomes activated and can then bind DNA. ... It is a broad-spectrum antibiotic that is effective against most bacteria, filamentous fungi, yeast, plant, and animal cells. ...

*Motilin receptor

Thielemans L, Depoortere I, Vanden Broeck J, Peeters TL (2002). "The motilin pharmacophore in CHO cells expressing the human ... 2001). "Demonstration of a functional motilin receptor in TE671 cells from human cerebellum". Brain Res. 895 (1-2): 119-28. doi ...

*ERCC1

CHO) cells were isolated, and this gene restored UV resistance to cells of complementation group 1. The human ERCC1 gene ... Wood RD, Burki HJ, Hughes M, Poley A (Feb 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell line ... Hayashi T, Takao M, Tanaka K, Yasui A (Jun 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... Mammalian cells with mutant ERCC1-XPF are moderately more sensitive than normal cells to agents (such as ionizing radiation) ...

*XPA

Hayashi T, Takao M, Tanaka K, Yasui A (Jun 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... DNA repair protein complementing XP-A cells is a protein that in humans is encoded by the XPA gene. Nucleotide excision repair ... "Analysis of 133 meioses places the genes for nevoid basal cell carcinoma (Gorlin) syndrome and Fanconi anemia group C in a 2.6- ...

*Leukotriene B4 receptor 1

Yokomizo T, Noiri E, Izumi T, Shimizu T (2003). "In vivo chemotaxis using CHO cells expressing human leukotriene B4 receptor". ... Kato K, Yokomizo T, Izumi T, Shimizu T (2000). "Cell-specific transcriptional regulation of human leukotriene B(4) receptor ... "Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells". J Biol Chem. 271 (31): 18363-7. doi:10.1074/jbc ... "Leukotriene B4 receptor antagonist LY293111 inhibits proliferation and induces apoptosis in human pancreatic cancer cells". ...

*Alpha-1D adrenergic receptor

2003). "alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP- ... positive regulation of cell proliferation. • cell proliferation. • positive regulation of smooth muscle contraction. • signal ... cell-cell signaling. • DNA metabolic process. • multicellular organism development. • adenylate cyclase-activating adrenergic ... Cell Genet. 66 (3): 170-1. doi:10.1159/000133693. PMID 8125015.. *. Forray C, Bard JA, Wetzel JM, et al. (1994). "The alpha 1- ...

*Lithium-sulfur battery

Nominal cell voltage. cell voltage varies nonlinearly in the range 2.5-1.7 V during discharge; batteries often packaged for 3 V ... Jeong, S. S.; Lim, Y.; Choi, Y. T.; Kim, K. W.; Ahn, H. J.; Cho, K. K. (2006). "Electrochemical properties of lithium sulfur ... Polysulfides are reduced on the cathode surface in sequence while the cell is discharging: S. 8 → Li. 2S. 8 → Li. 2S. 6 → Li. 2 ... Across a porous diffusion separator, sulfur polymers form at the cathode as the cell charges: Li. 2S → Li. 2S. 2 → Li. 2S. 3 → ...

*Oligosaccharyltransferase

... the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell ... "Cell. 136 (2): 272-83. doi:10.1016/j.cell.2008.11.047. PMC 2859625. PMID 19167329.. ... "J. Cell Biol. 161 (4): 715-25. doi:10.1083/jcb.200301043. PMC 2199356. PMID 12756234.. ...

*ERCC4

Wood RD, Burki HJ, Hughes M, Poley A (Feb 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell line ... If the stem cells at the base of the crypt express ERCC4 (XPF), generally all several thousand cells of the crypt will also ... Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV ... Nuclei of cells in the lamina propria, cells which are below and surround the epithelial crypts, largely show hematoxylin blue- ...

*Signal peptide

Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... "Unconventional mechanisms of protein transport to the cell surface of eukaryotic cells". Annual Review of Cell and ... Signal peptides function to prompt a cell to translocate the protein, usually to the cellular membrane. In prokaryotes, signal ... The process by which such secretory proteins gain access to the cell exterior is termed unconventional protein secretion (UPS ...

*Deacetylasperulosidic acid

The clastogen-suppressing effects of Tochu tea in CHO cells and mice". Mutation research. 388 (1): 7-20. doi:10.1016/s1383-5718 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line". Cancer Research. 61 (15): 5749-56. ...

*Gene expression

Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... this gives cells the flexibility to adapt to a variable environment, external signals, damage to the cell, and other stimuli. ... adhesion in tongue squamous cell carcinoma cells". Indian J. Med. Res. 129 (2): 154-63. PMID 19293442. Hanriot L, Keime C, Gay ... In a typical cell, an RNA molecule is only stable if specifically protected from degradation. RNA degradation has particular ...

*Nociceptinski receptor - Vikipedija, slobodna enciklopedija

Agonist-induced internalization and desensitization of the human nociceptin receptor expressed in CHO cells.". Cell. Mol. Life ... receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines.". Brain Res. Mol. Brain Res. 32 (2): ...

*Zygosity

"Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis". Cell. Elsevier BV. 14 (4): ... In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... "Molecular Cell Biology (4th ed.).. *^ Gupta, Radhey S.; Chan, David Y.H.; Siminovitch, Louis (1978). " ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ...

*Acute inhalation injury

Am J Respir Cell Mol Biol. 45:88-94. Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... Am J Respir Cell Mol Biol. 41:661-70 Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... 57:51-9. Tang PS, Mura M, Seth R, Liu M. (2008) Acute lung injury and cell death: how many ways can cells die? Am J Physiol 294 ... There are two types of alveolar epithelial cells - Type 1 pneumocytes represent 90% of the cell surface area, and are easily ...

*Positron emission tomography

CHO, Z. H., ERIKSSON L., and CHAN J.K., ``A circular ring transverse axial positron camera in Reconstruction Tomography in ... This tracer is a glucose analog that is taken up by glucose-using cells and phosphorylated by hexokinase (whose mitochondrial ... Although many investigators took this approach, James Robertson[66] and Zang-Hee Cho[67] were the first to propose a ring ... This means that FDG is trapped in any cell that takes it up until it decays, since phosphorylated sugars, due to their ionic ...

*Mir-345 microRNA precursor family

"Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7". Journal of Biotechnology. 151 (2 ... "Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin". ... a methylation-sensitive microRNA is involved in cell proliferation and invasion in human colorectal cancer". Carcinogenesis. 32 ... cellular motility and oxidative phosphorylation in neural precursors derived from human umbilical cord mesenchymal stem cells ...
BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
Microencapsulation of recombinant cells is a novel promising approach to tumor therapy in which therapeutic protein is sustainable and long-term delivered by microencapsulated cells. The semi-permeable membrane of microcapsule can protect cell from hosts immune rejection, increase the chemical stability of therapeutic protein and circumvent the problems of toxicity, limited half-lives and variation in circulating levels. Endostatin, a potent and specific angiogenesis inhibitor, could suppress the growth of primary and metastatic lesions in multiple murine tumor models. In this paper, APA microcapsules with high strength kept intact over 35 days and recombinant CHO cells kept the rapid proliferation viability and the continuous endostatin-expression function. The study of tumor treatment showed that the implantation of microencapsulated recombinant CHO cells decreased the neovascularization of tumor tissue by 59.4% and inhibited the B16 melanoma growth by 77.4%. Twenty days after tumor cell ...
BioAssay record AID 34248 submitted by ChEMBL: Displacement of [3H]NECA from human adenosine A2A receptor in stably transfected CHO cells.
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
serumm free culture of adherent cho cells - posted in Tissue and Cell Culture: Hi all, i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media for 72-96h. i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions which medium i can use to sustain 72-96h time? thnx
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
Glenmark signs full commercial-use license for Horizons gene-edited CHO cells Cambridge, UK, 30 September 2019: Horizon Discovery Group plc (LSE: HZD) ("Horizon"), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase ("GS") knockout Chinese Hamster Ovary (CHO) K1 cell line. Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Companys biomanufacturing processes. Martin Bertschinger, Deputy Director of Cell Sciences, Glenmark, explained: "After extensive evaluation, Horizons GS knockout CHO K1 cell line demonstrated consistently impressive performance. We generated clones with high levels of productivity and a favorable stability profile relative to our previous system. Incorporating this technology into our ...
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
Feichtinger J., Hernandez I., Fischer C., Hanscho M., Auer N., Hackl M., Jadhav V., Baumann M., Krempl P.M., Schmidl C., Farlik M., Schuster M., Merkel A., Sommer A., Heath S., Rico D., Bock C., Thallinger G.G., Borth N. (2016) Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time. Biotechn. Bioeng. 113:10:2241-2253. DOI: 10.1002/bit.25990. Gludovacz E., Maresch D., Bonta M., Szöllösi H., Furtmüller P.G., Weik R., Altmann F., Limbeck A., Borth N., Jilma B., Boehm T. (2016) Characterization of recombinant human diamine oxidase (rhDAO) produced in Chinese Hamster Ovary cells. J Biotechnol. 227, 120-130. Klanert G., Jadhav V., Shanmukam V., Diendorfer A.m Karbiener M., Scheideler M., Bort JH., Grillari J., Hackl M., Borth N. (2016) A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines. J Biotechnol. DOI 10.1016/j.jiotec.2016.03.022. Priola J.J., Calzadilla N., Baumann M., Borth N., Tate ...
Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
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CHO-Anti-Human TSHR F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TSHR F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
CHO-Anti-Human TGF beta 2 scFv stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TGF beta 2 scFv gene to allow expression of the scFv. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Read the latest Cho Oyu climbing news from RMI guides leading Cho Oyu climbing trips. Learn about Cho Oyu climbing conditions, routes, and more.
Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO (dimetylsulfoxide, sterile ...
There is no consensus as to whether NA activates the influx of extracellular Ca2+ influx in CHO-α1A, CHO-α1B, and CHO-α1D (Perez et al., 1993; Horie et al., 1995). Based on the results of the present study, we conclude that NA induces Ca2+ influx in CHO-α1A, CHO-α1B, and CHO-α1D (Figs. 1 and 4). Moreover, the magnitude of the transient increase and that of the sustained increase in [Ca2+]i were similar in all three cell types (Figs. 1 and 2). These results differ from the previous observation that the level of the NA-induced sustained increase in [Ca2+]i in CHO-α1D was smaller than that in CHO-α1A or CHO-α1B(Horie et al., 1995). However, this report showed that NA induced sustained increase in [Ca2+]i even in the absence of extracellular Ca2+ in CHO-α1A or CHO-α1B(Horie et al., 1995). Therefore, we have doubts about their data on monitoring of NA-induced increase in [Ca2+]i.. Because previous reports did not describe what types of Ca2+ channels are activated by NA in CHO-α1A, ...
One Liter of Irvine Scientific BalanCD™ CHO Growth A Medium and one CELLine 350 Bioreactor flask for a multiple harvest production run |p| This bundled product includes one liter of Irvine Scientific BalanCD CHO Growth A medium and a CELLine 350 Bi
|p| Three Liters of Irvine Scientific BalanCD™ CHO Growth A Medium and one CELLine 1000 Bioreactor flask for a multiple harvest production run |/p| |p| This bundled product includes three liters of Irvine Scientific BalanCD CHO Growth A medium and
هدف: ایجاد رده‏های سلولی دارای بیان بالا یک مرحله محدود کننده اصلی در روند تولید پروتئین‏های نوترکیب دارویی است. در این مطالعه اثر به‏کارگیری ناحیه متصل شونده به ماتریکس ژن اینترفرون بتای انسانی به‏همراه روش فعال‏سازی راه‏انداز از طریق پروتئین E1A 13S بر بیان فاکتور فعال کننده پلاسمینوژن بافتی (t-PA) در سلول‏های تخمدان هامستر چینی بررسی شده است. مواد و روش‏ها: ناحیه متصل شونده به ماتریکس در سمت ΄5 و΄3 واحد بیان کننده t-PA در ناقل pTPA کلون شد تا ناقل pMTPA به‏دست آید. پس از ترانسفکشن سلول‏ها با ناقل‏های pTPA و pMTPA، رده‏های سلولی پایدار ایجاد شده و میزان بیان t-PA برای
产品:invitrogen货号:K1483规格:2×10^6cells名称:GeneBLAzer®MC2R-CRE-bla-CHO-K1CellsTheGeneBLAzer®MC2R-CRE-bla-CHO-K1cellscontainthehumanmelanocortin-2receptor(MC2R),(Accession#NM_000529.1)andtheMe
Directed by Jong-bin Yoon. With Jung-woo Ha, Dong-won Gang, Sung-min Lee, Jin-woong Cho. A period action film centered on a militia group who turn against an unjust nobility.
Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more complex set-up needed.. In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. A comparison between Alternating Tangential Flow (ATF) and Tangential Flow Filtration (TFF) was performed using a recombinant CHO cell line producing a monoclonal antibody (mAb) as a model system. ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHO(BRI/rcTA)) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO(BRI/rcTA) pools producing high levels of recombinant proteins. Volumetric productivities of up to 900mg/L were reproducibly achieved for a Fc fusion protein and up to 350mg/L for an antibody after 14days post-induction in non-optimized fed-batch cultures. In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. ...
We have investigated whether the presence of a DNA repair enzyme, 06-methylguanine-DNA-methyltransferase (MGMT), affects the nature of spontaneous mutations in a mammalian cell line. We compared spontaneous mutations in the adenine phosphoribosyl transferase gene of a Chinese hamster ovary (CHO) cell line that expressed 14,000 MGMT molecules/cell with those in the parental CHO cells lacking this DNA repair activity. The mutation rate/cell/generation of the two CHO cell lines did not differ significantly. However, DNA sequence analysis of spontaneous mutations in the MGMT-proficient CHO cell line revealed a complex picture. No significant difference from the parental CHO cells was found in the number or type of deletions, frame-shifts, multiple substitutions, or insertions. The frequency of G:C to T:A transversions was elevated in MGMT-proficient CHO cells. Expression of the enzyme considerably reduced G:C to A:T transitions (25% versus 8.3%). This latter result is the first evidence that this ...
In a previous blog "Strategies for Improving Antibody Production in CHO Cells" three areas were identified where antibody production can be improved. In part one of the series titled "Utilizing Gene Synthesis to Improve Antibody Production in CHO Cells," we looked at gene synthesis as an alternative to classic cloning that offers a precise way to create a gene. In part two of the series titled "Strategies for Enhancing Media to Improve Antibody Production in CHO Cells," we examined the use of new media supplements to improve cell growth and productivity. In part three we will look at ways perfusion bioreactors can improve antibody production in CHO cell manufacturing.. In the biopharmaceutical industry there is an ongoing discussion about manufacturing capacity vs. demand for biologic drugs. Recently there has been increased concern because several biologic drugs have entered the final phases of the clinical pipeline. If many of these drugs receive approval, capacity could quickly become in ...
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. The Chinese hamster had been used in research since 1919 where they were used in place of mice for typing pneumococci. They were subsequently found to be excellent vectors for transmission of kala-azar (a.k.a. visceral leishmaniasis), facilitating leishmania research. In 1948, the Chinese hamster was first used in the United States for breeding in research laboratories. In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganians laboratory at the Boston Cancer Research Foundation and used it to ...
Kukkonen JP; G-protein-dependency of orexin/hypocretin receptor signalling in recombinant Chinese hamster ovary cells.; Biochem Biophys Res Commun, 2016 PubMed Europe PMC ...
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., "Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells." (1981). Subject Strain Bibliography 1981. 19 ...
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In interphase Chinese hamster ovary (CHO) cells, the centrosome is attached to the nucleus very firmly. This nuclear-centrosome complex is isolated as a coherent structure by lysis and extraction of cells with Triton X-100 in a low ionic strength medium. Under these conditions, the ultrastructure of the centrioles attached to the nucleus can be discerned by electron microscopy of whole-mount preparations. The structural changes of the centrioles as a function of the cell cycle were monitored by this technique. Specifically, centriolar profiles were placed into six categories according to their orientation and the length ratio of daughter and parent centrioles. The proportion of centrioles in each category was plotted as a frequency histogram. The morphological changes in the centriole cycle were characterized by three distinguishable events: nucleation, elongation, and disorientation. The progress of centrioles through these stages was determined in synchronous populations of cells starting from ...
BGI, the giant genomics institute located in Shenzhen, and GT Life Sciences of San Diego have published their collaborative study on the genomic sequence of the Chinese hamster ovary (CHO) K1 cell line in Nature Biotechnology. Over 70% of the recombinant therapeutic proteins sold today are manufactured using mammalian cells, primarily CHO cell lines. GT Life Sciences uses a metabolic modeling platform to design new products and processes for the life sciences field. It says a better understanding of the genome will speed development of new recombinant protein therapies. More details.... Share this with colleagues:   var switchTo5x=true;stLight.options({publisher:d7871f5b-67bc-4d30-b66f-1465d0b97213});
Previous findings have established G-6-P as an important mediator promoting HKII dissociation from mitochondria to cytosol (White and Wilson, 1990; Aleshin et al., 1998; Sebastian et al., 1999). In CHO cells overexpressing HKII, but not HKI, we found that glucose removal was associated with a delay in the subsequent rate of glucose phosphorylation (when Cyto B was present to block glucose efflux; John et al., 2011). Based on our prior detailed analysis in CHO cells (John et al., 2011), we attribute this delay to the activation of glycogenolysis when glucose was removed, which elevated G-6-P levels to both inhibit HKII and promote its dissociation from mitochondria. This interpretation was supported by our findings that IAA, which elevates G-6-P by inhibiting glycolysis distally (Fig. S2), caused HKII to dissociate from mitochondria in intact CHO cells with glucose present, and that exogenous G-6-P accelerated dissociation of HKs from mitochondria in permeabilized CHO cells (John et al., ...
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
We have isolated cis-diamminedichloroplatinum(II) (CDDP)-resistant variants, C/CDP-1 and C/CDP-2, from a Chinese hamster ovary (CHO) cell line after a stepwise exposure to increasing concentrations of CDDP, and a CDDP-sensitive revertant, R-1, from C/CDP-2 after continuous incubation for 5 months in the absence of CDDP. C/CDP-1 and C/CDP-2 showed 7- and 10-fold higher resistance to CDDP, respectively, compared to CHO cells. C/CDP-2 was cross-resistant to carboplatin, l-phenylalanine mustard (melphalan), and CdSO4, but not to other anticancer agents. Alkaline elution of DNA showed an increased amount of DNA interstrand cross-linking formation in CHO cells, but not in C/CDP-2 cells, when CHO and C/CDP-2 cells were cultured with CDDP. By contrast, alkaline elution of DNA showed increased formation of DNA cross-links when nuclei of C/CDP-2 cells were treated with CDDP. The activity of glutathione S-transferase (GST) of C/CDP-1 and C/CDP-2 was 4- and 6-fold higher than that of CHO cells, ...
Các gói cho thuê âm thanh của công ty cho thuê âm thanh ánh sáng: 1. Gói cho thuê âm thanh hội thảo - hội nghị (GÓI SỐ 1) của công ty cho thuê âm thanh ánh sáng: Gói cho thuê âm thanh hội thảo này được dùng cho các sự kiện âm thanh hội thảo hội nghị nhỏ trong phòng. https://images-blogger-opensocial.googleusercontent.com/gadgets/proxy?url=http%3A%2F%2Fsukienmattroi.com%2Fwp-content%2Fuploads%2F2017%2F03%2Fgoi-so-11.jpg&container=blogger&gadget=a&rewriteMime=image%2F* Giá gói cho
Activin A, Human, Recombinant, CHO Cells Activin A, Human, Recombinant, is a member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. A disulfide-linked homodimer of two 116-a.a. βA subunits. - Find MSDS or SDS, a COA, data sheets and more information.
Over the past decades, the increase in maximal cell numbers for the production of mammalian derived biologics has been in a large part due to the development of optimal feeding strategies. Engineering of the cell line is one of probable approaches for increasing cell numbers in bioreactor. We have demonstrated that the over-expression of the c-myc gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the control. The changes were attributed to a rapid transition into S-phase from a shortened duration of G1 phase and to the uncoupling of cell size from cell proliferation. To achieve the |70% increase in maximal cell density without additional supply of nutrients the cells underwent an overall reduction of 14% in size as well as a significant decrease in glucose and amino acid consumption rate. Consequently, the total biomass accumulation did not show a significant change from the control. The amount of hSEAP-hFc activity of the over
Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells Academic Article ...
Wong, D.C.F., Wong, K.T.K., Nissom, P.M., Yap, M.G.S., Heng, C.K. (2006). Targeting early apoptotic genes in batch and fed-batch CHO cell cultures. Biotechnology and Bioengineering 95 (3) : 350-361. [email protected] Repository. https://doi.org/10.1002/bit. ...
LinkedIn: Chinese Hamster Ovary cells, known as CHO, play an important role in modern medicine. NISTs new CHO peptide library will enable better production of treatments for psoriasis, cancer, hemophilia and leukemia.
In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. Optimised protein expression protocols provide the ability to load cells with greater quantities of DNA relative to the standard CHO protocol. As a result, average protein expression per cell is increased.
There are now several examples of single G protein-coupled receptors to which binding of specific agonists causes differential effects on the associated signaling pathways. The dopamine D2 receptor is of special importance because the selective activation of functional pathways has been shown both in vitro and in situ.
BalanCD® CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...
BalanCD® CHO Feed 1 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...
Eukaryotic expression vectors have been used successfully in viral LT-expressing cell lines (ie. COS) to clone cDNAs encoding proteins that can be detected through their bio-activity or reactivity with specific antibodies. Since Chinese hamster ovary cells (CHO) have been used extensively for the is... DRIVER (Chinese) ...
Mammalian cell culture-based manufacturing for biopharmaceuticals is perceived as an established technology. Many open questions remain.
Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.. ...
Learn about the latest developments of Sartorius Stedim Cellcas CHO Cell Line Generation platform. Contact our scientists today!
Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. The properties and performances obtained in this bioreactor with both filtration systems were studied.. ...
Dear Netters, I wish to express a clone of interest in mammalian cell lines. However, I want to be able to select for the transfected cells so I can maintain the plasmid in the cell line indefinitely. Which plasmids are good for this purpose? I have HeLa, COS, and CHO cells. Any suggestions are welcome. -fm ...
uri magnae xD Biodata Name asli : 조규현 Cho Kyuhyun Nama panggung : 규현 Kyuhyun Nama chinese : 趙奎賢, Zhao Kui Xian Dalam tulisan jepang : チョ・ギュヒョン Tanggal lahir : 3 Februari 1988 Tinggi : 180 cm Berat : 68 kg Golongan darah : A Pendidikan : Sekolah Menengah Shinchung, Sekolah Perguruan Tinggi Yumkwang, Universitas Kyunghee Casting : Chin Chin Singing Competition, 3rd place Tampilan pertama :
Bomsoo Cho is on CAP Network. CAP Network is a virtual workspace, to support collaboration among faculty, graduate students, postdocs and staff.
Yoon-Ok Kim (Kim YO), Myoung-Bum Choi (Choi MB), Youn-Kyeong Cho (Cho YK), Sun-Kyeong Sin (Sin SK), Song-Ja Kim (Kim SJ), Hyang-Ok Woo (Woo HO), Seoung-Hwan Kim (Kim SH), Hee-Shang Youn (Youn HS), Seon-Ju Kim (Kim SJ), Kook-Young Maeng (Maeng KY), Gyung-Hyuck Ko (Ko GH), Seung-Chul Baik (Baik SC), Woo-Kon Lee (Lee WK), Myung-Je Cho (Cho MJ), Kwang-Ho Lee (Lee KH ...
Find and order beads and products like this MagSi-S CHO 1.0 beads on www.antikoerper-online.de. Jetzt Produkt ABIN1721095 bestellen.
Eun-Young Cho (Cho EY), Young-Shil Park (Park YS), Dae-Hyung Lee (Lee DH), Ji Kyoung Park (Park JK), Sangrhim Choi (Choi SR), Sun Young Kim (Kim SY), Pil-Sang Jang (Jang PS), Dong-Gun Lee (Lee DG), Nak-Gyun Chung (Chung NG), Jong Hyun Kim (Kim JH), Dae-Chul Jeong (Jeong DC), Bin Cho (Cho B), Jae Gyun Hur (Hur JG), Jin Han Kang (Kang JH), Hack-Ki Kim (Kim HK ...
The purpose of this study is to assess the safety, pharmacokinetic and activity profiles of the ch14.18 antibody produced in cells of hamster origin (ch
Yong-Hoon Cho , Sun-Mo Kim , Anisha Gokarna , Ho-Sang Kwack , H. J. Kim , R. Ostroumov , K. L. Wang , Y. H. Kwon , T. W. Kang ...
About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
N,N,N,N-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.
We have isolated three independent Chinese hamster ovary cell mutants (B3853, I223, and M311) with temperature-sensitive, pleiotropic defects in receptor-mediated endocytosis. Activities affected at 41 degrees C include uptake via the D-mannose 6-phosphate receptor, accumulation of Fe from diferric transferrin, uptake of alpha 2-macroglobulin, compartmentalization of newly synthesized acid hydrolases, resistance to ricin, and sensitivity to diphtheria and Pseudomonas toxins and modeccin. The three mutants also displayed decreased sialylation of some secreted glycoproteins at 41 degrees C, reminiscent of the nonconditional mutant DTG1-5-4 that showed both endocytic and Golgi-associated defects (Robbins, A.R., C. Oliver, J.L. Bateman, S.S. Krag, C.J. Galloway, and I. Mellman, 1984, J. Cell Biol., 99:1296-1308). Phenotypic changes were detectable within 30 min after transfer of the mutants to 41 degrees C; maximal alteration of most susceptible functions was obtained 4 h after temperature shift. At ...
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks. ...
TY - JOUR. T1 - Erythropoietin fails to reverse the anemia in mice continuously exposed to tumor necrosis factor-alpha in vivo. AU - Clibon, U.. AU - Bonewald, Lynda. AU - Caro, J.. AU - Roodman, G. David. PY - 1990. Y1 - 1990. N2 - Tumor necrosis factor-α (TNF) is a monokine produced by activated macrophages that has cytotoxic and cytostatic effects on erythroid progenitor cells. We have recently shown that Chinese hamster ovary cells transfected with the human TNF gene and which constitutively express TNF induced a hypoproliferative anemia, mild thrombocytopenia, and mild leukocytosis when injected into nude mice. We have used this murine model to determine if treatment with recombinant human erythropoietin can prevent or ameliorate the anemia seen with long-term continuous exposure to high concentrations of TNF. Mice bearing TNF-producing tumors became anemic with hematocrits ranging from 30 to 32%. Treatment with recombinant human erythropoietin (100-1000 U/kg body weight three times per ...
The production of biopharmaceuticals for human use began in 1982 with recombinant insulin, and the development of new biopharmaceuticals has grew almost exponentially ever since. Over the past two decades, Chinese hamster ovary (CHO) cells have become the standard mammalian host cell line, with the expression and production of nearly 70% of all biopharmaceuticals [1, 2]. CHO cells provide efficient post-translational modifications, which allow the production of recombinant proteins with glycoforms that are both compatible with, and bioactive in humans [1]. CHO cells can also be easily manipulated genetically, which has become of great importance more recently [3]. These two characteristics are especially important in the production of biosimilars, where achieving the correct extent of similarity to the reference molecule is a great challenge. The nucleotide sequence of the gene that encodes amino-acid sequence of the desired protein is the same as for the reference molecule. In contrast, ...
Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy.
Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer rate. In this study, a Chinese hamster ovary cell line producing a recombinant antibody was cultivated in a 5L OSR and a 3L STR, both operated with or without pH control. Effects of bioreactor type and pH control on cell growth and metabolism and on recombinant protein production and glycosylation were determined. In pH-controlled bioreactors, the glucose consumption and lactate production rates were higher relative to cultures grown in bioreactors without pH control. The cell density and viability were higher in the OSRs than in the STRs, either with or without pH control. Volumetric recombinant antibody yields were not affected by the process conditions, and a glycan analysis of the antibody by mass spectrometry did not reveal major ...
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and plasticity in the context of genetic alterations.
Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell fed-batch culture processes. Cytotechnology, 69(1), 39-56. doi: 10.1007/s10616-016-0036-5 ...
Incubation with 2N-methyl-9-hydroxyellipticinium (NMHE) increases the killing of gamma-irradiated CHO cells. The main effect is observed in the case of exponentially growing cells which are also more sensitive to the drug alone than plateau phase cel
Cell adhesion is vastly important in cancer metastasis since the cells can only migrate once they have adhered and at some point become unbound from the extracellular matrix (ECM). The purpose of this experiment was to assess the amount of adhesion that occurs between cells and an ECM using various concentrations of the ECM. Several assays were performed with Chinese Hamster Ovary (CHO) cells to obtain a control. CHO cells have the integrin 51 receptors that allow the cells to adhere to the ligand Fibronectin in the ECM. B7 cells are CHO cells that have been stably transfected with the integrin IIb3. Since B7 cells contain the integrin IIb3 in addition to integrin 51, Fibrinogen was used as the ECM. Serum-starved cells were labeled with Calcein AM and readings were taken at an absorbance of 485 nm directly correlating to the amount of cells adhered to the ECM. To test the role of CIB1 in cell adhesion the B7 cells were transfected with Mock-pcDNA and CIB1 DNA. The ...
Two Chinese hamster ovary (CHO) cell variants differ substantially in their sensitivity to N-methyl-N -nitro-N-nitrosoguanidine (MNNG). The resistant clone (Cl 3) was isolated from the sensitive...
Rolling of GP Ibα-expressing cells on immobilized P-selectin. (A) CHO cells expressing either the GP Ib-IX complex or only GP Ibβ and GP IX were allowed to
I am looking for references to papers containing the time intervals spent in different phases of the cell cycle (ej., G0, G1, S, G2, M for eukaryotes) for different cells. In particular, I am interested in E. coli and CHO (Chinese Hamster Ovary cells), but any reference to studies of this kind for any typical cell will be useful.. Ill accept an answer containing a representative sample of references to the literature on this subject. Preferably recent papers (since 2010).. If you can provide the times spent in each phase but dont have references at hand, that will also be useful.. ...
A research team at the Northeast Agricultural University in Harbin managed to breed three transgenic pigs by injecting fluorescent green protein and a "bunch of other junk" into embryonic pigs, said Professor Liu Zhonghua. Liu wore a fancy white lab coat and had multiple degrees adorning his walls, so we assume he must be pretty smart ...
In the first experiment, cells growing in medium supplemented with the two combinations containing a trace element in common died at 2.5 % serum, while for the remaining combinations cell death occurred at a later stage, 0.625 % serum. Cell death was attributed to problems with the procedure of adaptation used in the first experiment, which were identified and corrected in the second experiment. The problems found and the procedure modifications implemented included the use of higher initial cell concentrations to allow the survival of an increased number of cells during the process; avoiding procedures that can be harsh to the cells, such as centrifugation and the use of enzymes (i.e. trypsin) due to a higher sensitivity of cells during adaptation; and allowing enough time in each step of the process for a complete cell adaptation.. After these modifications, in the second experiment, it was possible to observe that cells required a long time to adapt to each level of serum concentration, ...
We offer a plethora of recombinant proteins expressed in systems other than E.coli, human, and CHO cells that fit your research needs. Buy your recombinant proteins online from ProSci today.
Sundaram, H., Strange, Philip G. (1994) Characterisation of the human brain serotonin 5-HT1A receptor expressed in Chinese Hamster Ovary cells. Biochemical Society Transactions, 22 (1). S75-S75. ISSN 0300-5127. (doi:10.1042/bst022075s) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
And reactivity. inherited or sporadic. 20,150,151, Schulten et al. Determine the nitrogen in the residue by the viazyte of sulphuric vazyte viazyte (2.
Minkyoung Lee, Keunyoung Cheon, Boah Chae, Hyesung Hwang, Hyun-Kyung Kim, Youn-Jee Chung, Jae-Yen Song, Hyun-Hee Cho, Jang-Heub Kim, Mee-Ran Kim ...
Posted on small animals bit, but thought Id ask here. Im 14 weeks and as DP works away, Ive been cleaning out my DDa hamster cage all of this pre
In the last years, particular interest has been given to investigations concerning natural, effective and nontoxic compounds with radioprotective capacity in concert with increasing utilization of different types of ionizing radiation for various applications. Among them, propolis, a resinous mixture of substances collected by honey bees (Apis mellifera) has been considered promising since it presents several advantageous characteristics, i.e., anti-inflamatory, anticarcinogenic, antimicrobial and free-radical scavenging action. It is, therefore, a direct antioxidant that protects cells and organisms from the adverse effects of ionizing radiation. These relevant biological activities are mainly mediated by the flavonoids, present at relatively high concentrations in the propolis. Considering that the chemical composition and, consequently, the biological activity of propolis is variable according to the environmental plant ecology, the present study was conducted in order to evaluate the ...
Maurocalcine (MCa), initially identified from a Tunisian scorpion venom, defines a new member of the family of cell penetrating peptides by its ability to efficiently cross the plasma membrane. The initiating mechanistic step required for the cell translocation of a cell penetrating peptide implicates its binding onto cell surface components such as membrane lipids and/or heparan sulfate proteoglycans. Here we characterized the interaction of wild-type MCa and MCa K20A, a mutant analogue with reduced cell-penetration efficiency, with heparin (HP) and heparan sulfates (HS) through surface plasma resonance. HP and HS bind both to MCa, indicating that heparan sulfate proteoglycans may represent an important entry route of the peptide. This is confirmed by the fact that (i) both compounds bind with reduced affinity to MCa K20A and (ii) the cell penetration of wild-type or mutant MCa coupled to fluorescent streptavidin is reduced by about 50% in mutant Chinese hamster ovary cell lines lacking either all
Trypanosoma cruzi, the protozoan that causes Chagas heart disease, invades endothelial cells in vitro by activating the B-2 kinin receptor (B2R). Here, we demonstrate that mice infected with trypomastigotes develop potent edema after treatment with the angiotensin-converting enzyme (ACE) (or kininase II) inhibitor captopril. Experiments performed with specific kinin receptor (B2R/B1R) antagonists and knockout mice revealed that the early-phase (3-h) edema is mediated by the constitutive B2R, whereas the late-phase (24-h) response depends on stimulation of the up-regulated B1R. Given previous evidence that parasite invasion of cells expressing B2R is potentiated by captopril, we investigated the prerequisites for in vitro infection of Chinese hamster ovary cells overexpressing either B1R or B2R, human umbilical vein endothelial cells activated by lipopolysaccharide, and neonatal rat cardiomyocytes. Our results indicate that captopril potentiates parasite invasion regardless of the kinin ...
Acute alcohol use is associated with impaired immune responses and decreased proinflammatory cytokine production. Our earlier studies have shown that acute alcohol intake inhibits NF-kappaB DNA binding in an IkappaBalpha-independent manner. We report using human peripheral blood monocytes and Chinese hamster ovary cells transfected with CD14 cells that acute alcohol treatment in vitro exerts NF-kappaB inhibition by disrupting phosphorylation of p65. Immunoprecipitation of p65 and IkappaBalpha revealed that acute alcohol exposure for 1 h decreased NF-kappaB-IkappaBalpha complexes in the cytoplasm. Phosphorylation of p65 at Ser(536) is mediated by IkappaB kinase (IKK)beta and is required for NF-kappaB-dependent cellular responses. We show that acute alcohol treatment decreased LPS-induced IKKalpha and IKKbeta activity resulting in decreased phosphorylation of p65 at Ser(536). Furthermore, nuclear expression of IKKalpha increased after alcohol treatment, which may contribute to inhibition of NF-kappaB.
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
Somatic Cell and Molecular Genetic Analysis of Various Chinese Hamster Ovary (CHO) Mutant Cells Defective in Sterol-dependent Regulation of Cholesterol Biosynthesis and LDL Receptor Expression A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy by Mazahir Tahir Hasan Dartmouth College and Dartmouth Medical school Hanover, New Hampshire April 1993 ...
Somatic Cell and Molecular Genetic Analysis of Various Chinese Hamster Ovary (CHO) Mutant Cells Defective in Sterol-dependent Regulation of Cholesterol Biosynthesis and LDL Receptor Expression A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy by Mazahir Tahir Hasan Dartmouth College and Dartmouth Medical school Hanover, New Hampshire April 1993 ...
Two disintegrins with a high degree of amino acid sequence similarity, echistatin and eristostatin, showed a low level of interaction with Chinese hamster ovary (CHO) cells, but they bound to CHO cells transfected with αIIbβ3 genes (A5 cells) and to CHO cells transfected with αvβ3 genes (VNRC3 cells) in a reversible and saturable manner. Scatchard analysis revealed that eristostatin bound to 816000 sites per A5 cell (Kd 28 nM) and to 200000 sites (Kd 14 nM) per VNRC3 cell respectively. However, VNRC3 cells did not bind to immobilized eristostatin. Echistatin bound to 495000 sites (Kd 53 nM) per A5 cell and to 443000 sites (Kd 20 nM) per VNRC3 cell. As determined by flow cytometry, radiobinding assay and adhesion studies, binding of both disintegrins to A5 cells and resting platelets and binding of echistatin to VNRC3 cells resulted in the expression of ligand-induced binding sites (LIBS) on the β3 subunit. Eristostatin inhibited, more strongly than echistatin, the binding of three ...
Using sorting protocol based on a simple staining method for mitochondrial membrane potential we were able to isolate subclones from an established monoclonal antibody production cell line with significantly altered physiological properties. The subclone sorted for lower mitochondrial membrane potential had a faster growth rate, attained higher final cell concentrations in batch cultures, had lower glucose and glutamine uptake and lactate production rates as well as a higher specific production rate. The subclone sorted for high mitochondrial membrane potential on the other hand had a lower growth rate and final cell count, increased glucose and glutamine consumption and lactate production rates. These subclones can now be used for genomic or proteomic analysis of properties that characterise a cell line with efficient or inefficient metabolism. In addition, the method described is a valuable tool for cell line development and optimisation, offering the possibility to isolate subclones with both ...
Download Caffeine-induced alterations in non-histone chromosomal proteins of Chinese hamster ovary cells ebook by Susan Claire HarrisonType: pdf, ePub, zip, txt
The development of receptor-defective or -deficient mutants can be applied to the investigation of cell-matrix interactions including cell adherence and spreading. In the present study we developed a series of ethyl methyl sulfonate (EMS)-induced Chinese hamster ovary (CHO) cell mutants, which adhere to fibronectin but have impaired spreading characteristics. Using morphometric analysis, a significant suppression in the degree of cell spreading between the wild-type and the mutant cells (P less than 0.001) was seen. This inability of the mutant cells to spread adequately on fibronectin also resulted in a decreased number and diameter of stress fibers as compared to wild-type cells. The decreased cell spreading of the mutant cells was not due to inherent differences in cell size or volume, as determined by fluorescence-activated cell sorter (FACS) analysis. Since integrins, specifically the fibronectin receptor (alpha FN/beta 1), are important in cell adhesion and cell spreading, we carried out a ...
This study shows that the increase in blood pressure triggered by Ang II infusion can be completely prevented by the administration of soluble human rACE2. We used a highly purified soluble human rACE2 produced in the Chinese hamster ovary cell line, which has a calculated half-life in vivo of 8.5 hours (please see the supplementary Methods section). Our protocols involved acute studies in anesthetized animals and studies where rACE2 was given by osmotic minipumps for 3 days to conscious animals. Studies of long duration with human rACE2 administration were precluded because we found that mouse antihuman rACE2 antibodies developed over time, and this resulted in a decrease in serum ACE2 activity despite continued rACE2 infusion (please see the supplementary Results section).. As expected from the known effect of ACE2 on Ang II,2,3 human rACE2 was shown in vitro to cleave a single amino acid phenylalanine from Ang II, which led to the formation of Ang-(1-7). Recombinant ACE2 also acted on Ang I ...
Sou SN, Lee K, Nayyar K, Polizzi KM, Sellick C, Kontoravdi Cet al., 2017, Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation., Biotechnol Bioeng Transient gene expression (TGE) is a methodology employed in bioprocessing for the fast provision of recombinant protein material. Mild hypothermia is often introduced to overcome the low yield typically achieved with TGE and improve specific protein productivity. It is therefore of interest to examine the impact of mild hypothermic temperatures on both the yield and quality of transiently expressed proteins and the relationship to changes in cellular processes and metabolism. In this study, we focus on the ability of a Chinese hamster ovary cell line to galactosylate a recombinant monoclonal antibody (mAb) product. Through experimentation and flux balance analysis, our results show that TGE in mild hypothermic conditions led to a 76% increase in qP compared to TGE at 36.5°C in our ...
Ion channel conductance can be influenced by electrostatic effects originating from fixed surface charges that are remote from the selectivity filter. To explore whether surface charges contribute to the conductance properties of Kir2.1 channels, unitary conductance was measured in cell-attached recordings of Chinese hamster ovary (CHO) cells transfected with Kir2.1 channels over a range of K + activities (4.6-293.5 mM) using single-channel measurements as well as nonstationary fluctuation analysis for low K + activities. K + ion concentrations were shown to equilibrate across the cell membrane in our studies using the voltage-sensitive dye DiBAC 4 (5). The dependence of γ on the K + activity (a K ) was fit well by a modified Langmuir binding isotherm, with a nonzero intercept as a K approaches 0 mM, suggesting electrostatic surface charge effects. Following the addition of 100 mM N-methyl-D-glucamine (NMG + ), a nonpermeant, nonblocking cation or following pretreatment with 50 mM ...
Define CH3CHO. CH3CHO synonyms, CH3CHO pronunciation, CH3CHO translation, English dictionary definition of CH3CHO. n. A colorless, flammable liquid, C2H4O, used to manufacture acetic acid, perfumes, and drugs. n a colourless volatile pungent liquid, miscible with water,...
CDT was originally identified by Johnson and Lior (26, 28,29), in studies of enteric pathogens, as the soluble factor in bacterial culture supernatant fluids which caused the appearance of giant elongated Chinese hamster ovary (CHO) cells after prolonged incubation. Subsequent studies from their and other laboratories revealed that CDT is a novel toxic activity released by some E. coli strains (26, 51, 62) and Shigellaisolates (27, 42) and by many Campylobacterspecies (28, 40, 52). It has now been shown that CDT causes elongation followed by progressive cellular distention and cytotoxicity with certain mammalian cell lines (i.e., CHO, HeLa, HEp-2, and Vero) in vitro (29). Moreover, recent studies using HeLa cells have shown that E. coli CDT blocks the cell cycle at the G2/M transition, apparently by preventing Cdc2 protein kinase dephosphorylation and activation (12, 50, 75). In addition, E. coli CDT affects F-actin assembly by CHO cells in culture (4).. To date, the cdtABC gene clusters from a ...
CHO cells have become the most important cell system for the biotechnological production of pharmaceuticals. Reasons for that are described in the article The increasing potential of chinese hamster ovary cells. A critical feature of a cell line used for production of therapeutics is to be clonal, which means origination from a single cell, to ensure a homogenous therapeutic product. The demonstration of clonal derivation can be a big effort for the pharmaceutical industry, but does it really ensure a homogenous product quality?. ...
Substrate effects on the activation kinetics of Chinese hamster dihydrofolate reductase by p-chloromercuribenzoate (pCMB) have been studied. On the basis of the kinetic equation of substrate reaction in the presence of pCMB, all modification kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present study indicate that the modification of Chinese hamster dihydrofolate reductase by pCMB shows single-phase kinetics, and that changes in the enzyme activity and tertiary structure proceed simultaneously during the modification process. Both substrates, NADPH and 7,8-dihydrofolate, protect dihydrofolate reductase against modification by pCMB. In the presence of a saturating concentration of NADPH, the value of kcat for 7,8-dihydrofolate in the enzyme-catalysed reaction increased four-fold on modification of Cys-6, accompanied by a two-fold increase in Km for the modified enzyme. The utilization of the binding energy of a ...
Translocation of a nascent protein from the cytosol into the ER mediated by its signal peptide is a critical step in protein secretion. The aim of this work was to develop a platform technology to optimize the signal peptides for high level production of therapeutic antibodies in CHO cells. A database of signal peptides from a large number of human immunoglobulin (Ig) heavy chain (HC) and kappa light chain (LC) was generated. Most of the HC signal peptides contain 19 amino acids which can be divided into three domains and the LC signal peptides contain 22 amino acids. The signal peptides were then clustered according to sequence similarity. Based on the clustering, 8 HC and 2 LC signal peptides were analyzed for their impacts on the production of 5-top selling antibody therapeutics, namely, Herceptin, Avastin, Remicade, Rituxan, and Humira. The best HC and LC signal peptides for producing these 5 antibodies were identified. The optimized signal peptides for Rituxan is 2-fold better compared to its
Ameco Research has announced the addition of the ldquo;Global Recombinant Hamster Ovary Cell (CHO) Hepatitis B Vaccine Market: Global Industry Size, Share, Trends and Forecast, 2019-2025 report to their offering.ldquo;Global Recombinant Hamster Ovary Cell (CHO) Hepatitis B Vaccine Market 2019-2025rdquo; provides, w...
The results of in vitro mutagenicity tests with bromodichloromethane were mixed. Bromodichloromethane did not induce mutations in any of several tester strains of Salmonella typhimurium, with or without exogenous metabolic activation (S9 liver enzymes). In contrast to the negative results in Salmonella, tests for mutation induction in mouse lymphoma L5178Y/tk+/-cells were positive in the presence of induced rat liver S9; no mutagenic activity occurred in tests conducted without S9. In cytogenetic tests with cultured Chinese hamster ovary cells, bromodichloromethane induced a small increase in sister chromatid exchanges (SCEs) in one of four trials conducted in the presence of induced rat liver S9 enzymes; no significant increase in SCEs occurred without S9, and no induction of chromosomal aberrations occurred in bromodichloromethane-treated Chinese hamster ovary cells with or without S9.. Results of in vivo tests for chromosomal damage were negative. No increases in the frequency of ...
TY - JOUR. T1 - Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells. AU - Yoshimori, Tamotsu. AU - Keller, Patrick. AU - Roth, Michael G.. AU - Simons, Kai. PY - 1996/4. Y1 - 1996/4. N2 - The question of how membrane proteins are delivered from the TGN to the cell surface in fibroblasts has received little attention. In this paper we have studied how their post-Golgi delivery routes compare with those in epithelial cells. We have analyzed the transport of the vesicular stomatitis virus G protein, the Semliki Forest virus spike-glycoprotein, both basolateral in MDCK cells, and the influenza virus hemagglutinin, apical in MDCK cells. In addition, we also have studied the transport of a hemagglutinin mutant (Cys543Tyr) which is basolateral in MDCK cells. Aluminum fluoride, a general activator of heterotrimeric G proteins, inhibited the transport of the basolateral cognate proteins, as well as of the hemagglutinin mutant, from the TGN to the cell surface in BHK and ...
Production of soluble amyloid peptide precursor (APP) and amyloid peptide (A beta) was measured in CHO cells transfected by the wild-type APP 695 cDNA sequence or by the same sequence carrying missense mutations associated with familial Alzheimers disease in Sweden. Deletion of the C-terminal domain of the protein corresponding to residues 654 to 695 of APP 695 not only inhibited very significantly the internalization of APP at 37 degrees C, but also led to the secretion of an uncleaved APP in the culture medium of CHO cells. This deletion did not affect A beta production from the Swedish APP but was able to inhibit the production of the wild-type APP. These results demonstrate that, in CHO cells, the internalization of the wild-type APP is needed for A beta production, while the production of the amyloid peptide from Swedish APP is independent of the internalization process. ...

Recombinant Protein Therapeutics from CHO Cells -- 20 Years and Counting | AIChERecombinant Protein Therapeutics from CHO Cells -- 20 Years and Counting | AIChE

The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ... Recombinant Protein Therapeutics from CHO Cells -- 20 Years and Counting. The CHO cell is at its height of technological ... This is a major driver in further understanding Chinese Hamster Ovary (CHO) cell biology, which is the cell line of choice for ... The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ...
more infohttps://www.aiche.org/resources/publications/cep/2007/october/recombinant-protein-therapeutics-cho-cells-20-years-and-counting

Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells | Sigma-AldrichMgat1-Disrupted Chinese Hamster Ovary (CHO) Cells | Sigma-Aldrich

reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. ... The host cell line (CHOZN® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. Mild ... reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1 ... Overexpressing Mgat1 in wildtype CHO cells did not lead to increased sialylation ...
more infohttps://www.sigmaaldrich.com/technical-documents/articles/biology/mgat1-disrupted-chinese-hamster-ovary-cells.html

Cricetulus griseus strain:CHO K1 cell line (ID 69991)  - BioProject - NCBICricetulus griseus strain:CHO K1 cell line (ID 69991) - BioProject - NCBI

CHO K1 cell line Cricetulus griseus strain:CHO K1 cell line. The Genomic Sequence of the Chinese Hamster Ovary 1 (CHO) K1 cell ... Cricetulus griseus strain:CHO K1 cell line (Chinese hamster). The Genomic Sequence of the Chinese Hamster Ovary 1 (CHO) K1 cell ... cell lines are preferred host cells for therapeutic protein production. More.... Chinese Hamster Ovary (CHO) cell lines are ... The availability of the CHO genome ushers in an era of genome-scale science and metabolic engineering for CHO and CHO-produced ...
more infohttps://www.ncbi.nlm.nih.gov/bioproject/69991

Chinese Hamster Ovary Cells (CHO-K1 Line) | MicroscopyUChinese Hamster Ovary Cells (CHO-K1 Line) | MicroscopyU

A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros ... Chinese Hamster Ovary Cells (CHO-K1 Line). A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of ...
more infohttps://www.microscopyu.com/gallery-images/chinese-hamster-ovary-cells-cho-k1-line

WikiGenes - CHO CellsWikiGenes - CHO Cells

Importantly, in cell-cell adhesion assays between CD2+ Jurkat T cells and CD48- or CD59-transfected CHO cells, there was no ... Gene context of CHO Cells. *Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3(-/-) chicken DT40 cells, indicating ... cells [15].. *Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the ... The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell ...
more infohttps://www.wikigenes.org/e/mesh/e/10946.html

Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells.  - PubMed - NCBIAntagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells. - PubMed - NCBI

... we have transfected each of these genes into Chinese hamster ovary cells (CHO-K1) and have established stable cell lines ... Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells.. Buckley NJ1, Bonner TI, Buckley ... National Institute of Mental Health, Laboratory of Cell Biology, Bethesda, Maryland 20892.. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/2704370

cho-lec2 Cells | Thermo Fisher Scientific - AUcho-lec2 Cells | Thermo Fisher Scientific - AU

CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. Transport of ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... defective compared to vesicles from wild-type cells, whereas transport of other nucleotide sugars was normal. These cells do ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ...
more infohttps://www.thermofisher.com/au/en/home/technical-resources/cell-lines/c/cell-lines-detail-191.html

cho-lec1 Cells | Thermo Fisher Scientific - JPcho-lec1 Cells | Thermo Fisher Scientific - JP

CHO glycosylation mutants. CHO-Lec1 cells completely lack complex and hybrid-type N-glycans on glycoproteins.. ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ...
more infohttps://www.thermofisher.com/jp/en/home/technical-resources/cell-lines/c/cell-lines-detail-202.html

EX-CELL® Advanced™ CHO Fed-batch Medium | Sigma-AldrichEX-CELL® Advanced™ CHO Fed-batch Medium | Sigma-Aldrich

EXCELL Advanced CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ... CHO Feed 1 for superior platform performance in fed-batch cultures on all industrial CHO cell lineages (CHO-S, DuxB11, DG44, ... Determine the correct volume of cell culture to inoculate a new flask at a starting cell density of 2-3 x105 cells/ml in a ... EX-CELL® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ...
more infohttps://www.sigmaaldrich.com/technical-documents/articles/biology/ex-cell-advanced-cho-fed-batch-medium.html

727e) A Model-Based Strategy for Understanding and Improving Recombinant Protein Glycosylation in CHO Cells | AIChE727e) A Model-Based Strategy for Understanding and Improving Recombinant Protein Glycosylation in CHO Cells | AIChE

727e) A Model-Based Strategy for Understanding and Improving Recombinant Protein Glycosylation in CHO Cells. ... Our platform has been validated against experimental data from two IgG-producing CHO cell lines representing all aforementioned ... Specifically, the higher scale model is a cell culture dynamics (CCDyn) model, which describes cell growth and the variation of ... CHO) cells, the workhorse of the biopharmaceutical industry. Our platform constitutes a multi-scale model describing the ...
more infohttps://www.aiche.org/conferences/aiche-annual-meeting/2015/proceeding/paper/727e-model-based-strategy-understanding-and-improving-recombinant-protein-glycosylation-cho-cells

cMyc increases cell number through uncoupling of cell division from cell size in CHO cells | Directory of Open Access JournalscMyc increases cell number through uncoupling of cell division from cell size in CHO cells | Directory of Open Access Journals

... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells in DOAJ. DOAJ is an online ... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells. BMC Biotechnology. 2009;9(1):76 DOI ... It is shown that the manipulation of cell cycle kinetics and indirectly cell metabolism gives higher cell densities in CHO ... gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the ...
more infohttps://doaj.org/article/ed5b13ff320c46559dc38a38b4e03ae3?frbrVersion=5

Stable expression of a functional rat angiotensin II (AT1A) receptor in CHO-K1 cells: Rapid desensitization by angiotensin II |...Stable expression of a functional rat angiotensin II (AT1A) receptor in CHO-K1 cells: Rapid desensitization by angiotensin II |...

In AT1A receptor transfected CHO-K1 cells, angiotensin II (10−9 M) stimulated a rapid increase in cytosolic free calcium that ... Stable expression of a functional rat angiotensin II (AT1A) receptor in CHO-K1 cells: Rapid desensitization by angiotensin II. ... To address these problems, we expressed the recombinant AT1A receptor in CHO-K1 cells. The stably transfected receptor was ... high-level transfectant of the AT1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following ...
more infohttps://link.springer.com/article/10.1007%2FBF00926885

Sack: Freezing CHO cells - OpenWetWareSack: Freezing CHO cells - OpenWetWare

Gently resuspend cell pellet in 2 ml freezing medium*.. * Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, ... Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. ... Freezing CHO Cells. Christina McGee 4-5-2012. * Grow 10 cm dish ... Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO ( ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Sack:_Freezing_CHO_cells&oldid=667947" ...
more infohttps://openwetware.org/wiki/Sack:_Freezing_CHO_cells

Serum-free large-scale transient transfection of CHO cellsSerum-free large-scale transient transfection of CHO cells

Keywords: Animals ; Bioreactors ; CHO Cells/*physiology ; Cell Culture Techniques/methods ; Cell Proliferation ; Cell Survival ... CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear ... Serum-free large-scale transient transfection of CHO cells. Derouazi, M.; Girard, P.; Van Tilborgh, F.; Iglesias, K.; Muller, N ... The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected ...
more infohttps://infoscience.epfl.ch/record/105019

AequoZen FroZen cells, Secretin, Human Recombinant, CHO-K1 | PerkinElmerAequoZen FroZen cells, Secretin, Human Recombinant, CHO-K1 | PerkinElmer

... simple luminescent calcium flux assays using irradiated AequoScreen cells tranfected with human secretin receptor - no cell ... in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted ... The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we ... Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you ...
more infohttps://www.perkinelmer.com/product/glucagon-secretin-aequozen-es-712-af

CHO-K1/NK2 Stable Cell LineCHO-K1/NK2 Stable Cell Line

Catalog Products » Other Products » Stable Cell Lines » Human Recombinant NK2 Tachykinin Receptor Stable Cell Line ... Human Recombinant NK2 Tachykinin Receptor Stable Cell Line Description. Tachykinins are peptides sharing a common C-terminal ...
more infohttps://www.genscript.com/molecule/M00200-NK2.html

CHO-K1/BB2 Stable Cell LineCHO-K1/BB2 Stable Cell Line

GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. ... GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays.. ... Catalog Products » Other Products » Stable Cell Lines » Human Recombinant BB2 Bombesin Receptor Stable Cell Line ... Human Recombinant BB2 Bombesin Receptor Stable Cell Line Description. The bombesin receptor family is a member of the G protein ...
more infohttps://www.genscript.com/molecule/M00182-BB2.html

Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35 |...Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35 |...

... cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration ... Hiller GW, Ovalle AM, Gagnon MP, Curran ML, Wang WG (2017) Cell-controlled hybrid perfusion fed-batch CHO cell process provides ... CHO Site-specific integration CRISPR/Cas9 Cell line development C12orf35 Electronic supplementary material. The online version ... Li S, Gao X, Peng R, Zhang S, Fu W, Zou F (2016) FISH-based analysis of clonally derived CHO cell populations reveals high ...
more infohttps://link.springer.com/article/10.1007%2Fs00253-018-9021-6

Research Associate/Senior Research Associate - CHO Stable Cell Line Development job with NGM Biopharmaceuticals, Inc. | 1877869Research Associate/Senior Research Associate - CHO Stable Cell Line Development job with NGM Biopharmaceuticals, Inc. | 1877869

CHO Stable Cell Line Development in Science/R&D with NGM Biopharmaceuticals, Inc.. Apply Today. ... Broad experience in cell biology, including mammalian cell culture, cell transfection, and cell-based functional assays ... Develop stable cell lines including clone screening, single cell cloning, and fed-batch production ... Evaluate the CHO CLD and production process with varies media. *Design studies and interpret results of development, ...
more infohttps://www.biospace.com/job/1877869/research-associate-senior-research-associate-cho-stable-cell-line-development/

serumm free culture of adherent cho cells - Tissue and Cell Culture - BioForumserumm free culture of adherent cho cells - Tissue and Cell Culture - BioForum

i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ... posted in Tissue and Cell Culture: Hi all, ... cho cells as that may change some properties of the cho cells. ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ...
more infohttp://www.protocol-online.org/forums/topic/26144-serumm-free-culture-of-adherent-cho-cells/

Adhering CHO cell culture - Tissue and Cell Culture - BioForumAdhering CHO cell culture - Tissue and Cell Culture - BioForum

... cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day- ... posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) ... Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i dont ... Have you done a viability assay to ensure that the floating cells are, in fact, dead?. CHO cells are damned hardy and will ...
more infohttp://www.protocol-online.org/forums/topic/15050-adhering-cho-cell-culture/

Comparative pharmacology of human adenosine receptor subtypes - characterization of stably transfected receptors in CHO cells.Comparative pharmacology of human adenosine receptor subtypes - characterization of stably transfected receptors in CHO cells.

In this study all human subtypes were stably transfected into Chinese hamster ovary (CHO) cells in order to be able to ... CHO Cells / metabolism*. Cricetinae. Guanylate Cyclase / metabolism. Humans. Phenethylamines / pharmacology. ... In this study all human subtypes were stably transfected into Chinese hamster ovary (CHO) cells in order to be able to study ... The CHO cells with stably transfected adenosine receptors provide an identical cellular background for such a pharmacological ...
more infohttp://www.biomedsearch.com/nih/Comparative-pharmacology-human-adenosine-receptor/9459566.html

cAMPZen Frozen cells, Somatostatin sst2a, Human Recombinant, CHO-K1 | PerkinElmercAMPZen Frozen cells, Somatostatin sst2a, Human Recombinant, CHO-K1 | PerkinElmer

... simple binding and functional assays using irradiated cells transfected with human somatostatin (sst2a) receptor - no cell ... in CHO-K1 host cell. We provide one vial of frozen cells (2.5 million cells/vial). Some of our Frozen cells may be restricted ... Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required ... Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you ...
more infohttps://www.perkinelmer.com/product/somatostatin-sst2a-campzen-es-521-cf
  • A functional hamster MGAT1 was overexpressed in an Mgat1-disrupted IgG producing cell line (Mgat1 KO37). (sigmaaldrich.com)
  • The host cell line (CHOZN ® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. (sigmaaldrich.com)
  • Mgat1KO37OE4 expresses Mgat4b significantly higher, while OE12's Mgat4b is significantly lower, than the non-ZFN transfected cell line. (sigmaaldrich.com)
  • Mgat1KO37 has significantly decreased Mgat4b than non-ZFN transfected cell line where it was derived from. (sigmaaldrich.com)
  • The host cell line that does not express IgG has significantly higher Mgat4b than the IgG-expressing non-ZFN transfected line. (sigmaaldrich.com)
  • GenScript's BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. (genscript.com)
  • However, by conventional random integration strategy, development of a high-expressing and stable recombinant CHO cell line has always been a difficult task due to the heterogenic insertion and its caused requirement of multiple rounds of selection. (springer.com)
  • In comparison with a random integration control, all the targeted integration cell lines showed higher productivity, among which C12orf35 locus was the most advantageous in both productivity and cell line stability. (springer.com)
  • Damavandi N, Raigani M, Joudaki A, Davami F, Zeinali S (2017) Rapid characterization of the CHO platform cell line and identification of pseudo attP sites for PhiC31 integrase. (springer.com)
  • CHO Stable Cell Line Development job with NGM Biopharmaceuticals, Inc. (biospace.com)
  • They grow quite well according to their passage number, even the cell line with 86 passages grows although slower than the one on passage 5. (protocol-online.org)
  • By the way, it also happens to the parental CHO-K1 line, so that's even trickier. (protocol-online.org)
  • Engineering of the cell line is one of probable approaches for increasing cell numbers in bioreactor. (doaj.org)
  • cell line was found to be within 0.7% of the control. (doaj.org)
  • over-expressing cell line did not increase with the increase in cell number. (doaj.org)
  • Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, date and your initials. (openwetware.org)
  • Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine Cytidylyltransferase. (doaj.org)
  • The CHO-MT58 cell line expresses a mutant variant of CCT, and displays a thermo-sensitive phenotype. (doaj.org)
  • This also provide an explanation for the observed thermo-sensitive phenotype of CHO-MT58 cell line. (doaj.org)
  • Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l. (frontiersin.org)
  • Goh JB, Ng SK (2017) Impact of host cell line choice on glycan profile. (springer.com)
  • A Chinese hamster ovary cell line (ACC098) expressing the human 5-HT 1B receptor was used as the receptor source. (bio-medicine.org)
  • POTELLIGENT([R]) Technology involves the reduction of the amount of fucose in the carbohydrate structure of an antibody using a proprietary fucosyl transferase-knockout CHO cell line as a production cell. (thefreedictionary.com)
  • The first EX-CELL Advanced product is the batch media system developed for a range of widely used industrial CHO cell lines, including SAFC's CHOZN cell line. (thefreedictionary.com)
  • With so many variants of the CHO cell line and so many generic media and associated supplements, it seems difficult to just pick one media and associate it with any CHO-derived cell line. (thefreedictionary.com)
  • We offer an extensive breadth of process development capabilities from cell line and strain development, using our proprietary pAVEway[TM] and CHO cell line systems, to process development, analytical development, clinical and commercial manufacturing. (thefreedictionary.com)
  • Cell Biologist with experience in CHO cell line development needed for growing biotechnology company. (thefreedictionary.com)
  • CHO-HuCLCN1 cell line is clonally-derived from a CHO cell line, which has been transfected with a human chloride channel, voltage-sensitive 1 (CLCN1) to allow stably express of the human CLCN1. (creativebiomart.net)
  • It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. (creativebiomart.net)
  • Protein expression: CLCN1 expression in this cell line has been validated by WB.3. (creativebiomart.net)
  • CHO-HuTRPM4 cell line is clonally-derived from a CHO cell line, which has been transfected with a human transient receptor potential cation channel, subfamily M, member 4 (TRPM4) to allow stably express of the human TRPM4. (creativebiomart.net)
  • The purpose of this project was to investigate techniques that may be successfully applied to improve the characteristics of a bioprocess relevant mammalian cell line such as CHO (Chinese Hamster Ovary). (dcu.ie)
  • Using this technique we successfully isolated a number of mutant clones with average maximum viable cell densities 53% greater than a control cell line when placed through the same stress selective procedure. (dcu.ie)
  • In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. (scielo.br)
  • Finally we designed single guide RNAs to target Cas9 to the miR-7a-5p genomic locus to disrupt miR-7 in order to enhance growth of a CHO-K1 cell line producing an IgG-1. (dcu.ie)
  • The interaction of protective antigen (PA), a component of the anthrax toxin, with receptors on the Chinese hamster ovary cell line CHO-K1 was characterized. (asm.org)
  • Having established that the fluid dynamics satisfied critical requirements for mammalian cell culture, we carried out further studies to evaluate its cell culture performance using a Chinese hamster ovary (CHO)-S cell line and its comparative performance and scalability for cell culture studies against traditional stirred-tank bioreactors. (bioprocessintl.com)
  • The GeneBLAzer® MC2R-CRE-bla-CHO-K1 cells contain the human melanocortin-2 receptor (MC2R), (Accession # NM_000529.1) and the Melanocortin-2 receptor accessory protein (MRAP), (Accession #NM_178817.3) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. (caigou.com.cn)
  • Horizon Discovery Group plc (LSE: HZD) ("Horizon"), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase ("GS") knockout Chinese Hamster Ovary (CHO) K1 cell line. (horizondiscovery.com)
  • Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Company's biomanufacturing processes. (horizondiscovery.com)
  • After extensive evaluation, Horizon's GS knockout CHO K1 cell line demonstrated consistently impressive performance. (horizondiscovery.com)
  • We are extremely encouraged by the adoption of our GS knockout CHO K1 cell line by Glenmark. (horizondiscovery.com)
  • These licenses have been taken as a result of an unmatched combination of high performance, transparent cell line history, supporting documentation, and attractive licensing terms. (horizondiscovery.com)
  • The system includes the GS knockout CHO K1 cell line, a comprehensive package of supporting documentation, and an expression vector supplied under license from DNATwoPointO, Inc.. This biomanufacturing platform allows these companies to move from the DNA sequence of their potential biotherapeutic to clinical manufacturing as simply and rapidly as possible. (horizondiscovery.com)
  • Characterization and investigation of CHO cell metabolism in a quick and simple way could boost process and cell line development. (springer.com)
  • CHO-HuGABRA5/GABRB2/GABRG2 cell line is clonally-derived from a CHO cell line, which has been transfected with a human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), a human gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2) and a human gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2) to allow stably express of the human GABRA5, GABRB2 and GABRG2. (creativebiomart.net)
  • Protein expression: GABRA5/GABR2/GABRG2 expression in this cell line has been validated by WB.3. (creativebiomart.net)
  • Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool. (pubmedcentralcanada.ca)
  • Here, we decided to take advantage of a biotechnology-relevant production cell line, Chinese hamster ovary (CHO) DG44, to establish a serum-free, stable, CR9114-like (CR9114L, a generic version of CR9114) antibody-producing cell line for a steady supply of this mAb with low batch to batch variation. (pubmedcentralcanada.ca)
  • The CHO DG44 cell line, in which both alleles are dihydrofolate reductase (DHFR) negative, was created in 1980ies by ionizing radiation [ 7 ]. (pubmedcentralcanada.ca)
  • Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. (diva-portal.org)
  • As a result, there are several limitations in the range of products that can be expressed and secreted by this cell line. (selexis.com)
  • Selexis SA, a JSR Life Sciences Company, is the global leader in cell line development with best-in-class modular technology and highly specialized solutions that enable the life sciences industry to rapidly discover, develop and commercialize innovative medicines and vaccines. (selexis.com)
  • Our global partners are utilizing Selexis cell line development technologies to advance more than 115 drug products in clinical development and the manufacture of five commercial products. (selexis.com)
  • In addition, expressing PKCbeta in a parietal endoderm cell line caused these cells to retrodifferentiate into stem cells. (aacrjournals.org)
  • We believe that our cell line development technologies offers solutions that no one else is able to provide. (selexis.com)
  • Selexis' innovation and expertise have enabled the development and ongoing optimization of the most robust and flexible mammalian cell line-based protein expression platform in the industry. (selexis.com)
  • Styrylpyrone Derivative (SPD) induces apoptosis in a caspase-7-dependent manner in the human breast cancer cell line MCF-7. (nih.gov)
  • Here, we used the caspase-3-deficient MCF-7 cell line. (nih.gov)
  • The Cellvento™ CHO platform includes cell line and process-specific media and feed compositions, allowing selection of the optimal media formulation for a given application. (merckmillipore.com)
  • The range of products also offers customers the flexibility to choose the most suitable product to achieve the best possible performance results for their specific cell line. (merckmillipore.com)
  • First, wild-type CHO was compared with tunicamycin-treated CHO and Lec9.4a cells, a mutant CHO cell line which shows 50% of wild-type glycosylation levels. (jhu.edu)
  • The Freedom™ CHO-S™ Kit includes all major components to complete the cell line development work flow: cGMP banked CHO-S™ cells, a cloning vector for two genes, transfection reagent, competent cells, serum-free cell culture media and reagents. (probiogen.de)
  • ProBioGen is a renowned Contract Development and Manufacturing Organization (CDMO) and Technology Provider, focusing on cell line engineering, process development (upstream- and downstream) and GMP manufacturing of biopharmaceuticals. (probiogen.de)
  • Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. (diva-portal.org)
  • In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. (diva-portal.org)
  • Here we describe the application of a closed-loop control scheme for the long-term cultivation of CHO cells in a high cell density (35 - 40 million cells/ml) perfusion process. (bioprocessintl.com)
  • June 1, 2015, Darmstadt, Germany - Merck Millipore, the Life Science division of Merck , today announced the launch of its Cellvento™ CHO platform of cell culture media and companion feed formulations for batch, fed-batch and perfusion applications. (merckmillipore.com)
  • Our results demonstrated the feasibility of C12orf35 as the target site for exogenous gene integration, and strongly suggested that C12orf35 targeted integration mediated by CRISPR/Cas9 is a reliable strategy for the rapid development of recombinant CHO cell lines. (springer.com)
  • Ding K, Han L, Zong H, Chen J, Zhang B, Zhu J (2017) Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein. (springer.com)
  • gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the control. (doaj.org)
  • As ActiPro medium and Cell Boost 7a and 7b supplements do not contain hypoxanthine or thymidine (HT), their formulation supports the dihydrofolate reductase (DHFR) gene amplification and selection system. (labonline.com.au)
  • We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. (portlandpress.com)
  • Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. (portlandpress.com)
  • Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. (portlandpress.com)
  • A considerable amount of optimisation work was performed to establish the CRISPR system for use in the lab, initially using eGFP as model target gene in CHO cells. (dcu.ie)
  • CellSensor® CRE-bla CHO-K1 cells (Cat # K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP Response Element (CRE). (caigou.com.cn)
  • Built upon more than a decade of experience in the engineering of cell lines, Horizon offers an unmatched portfolio of tools and services to help scientists gain a greater understanding of gene function, identify genetic drivers behind human disease, deliver biotherapeutics, cellular and gene therapies for precision medicine as well as develop and validate diagnostic workflows. (horizondiscovery.com)
  • Horizon's solutions enable almost any gene to be altered, or its function modulated, in human and other mammalian cell lines. (horizondiscovery.com)
  • Point mutation in the Virbrio cholerae O139 cef (CHO cell elongating factor) gene alters the. (deepdyve.com)
  • Shalu, O. 2012-02-20 00:00:00 Sequencing of the cef (CHO cell elongating factor) gene of Vibrio cholerae serogroup O139 revealed one nucleotide substitution (T to C at nucleotide 2015) as compared to cef of classical V. cholerae O1 and two substitutions (GT to AC at nucleotides 2014-2015) as compared to cef of V. cholerae O1 El Tor. (deepdyve.com)
  • Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch. (physiciansweekly.com)
  • Based on these results, we conclude that PKCbeta and PKCalpha are key targets for RA-regulated gene expression, that PKCalpha plays an important, active role in inducing and maintaining the parietal endoderm phenotype, and that PKCbeta activity is incompatible with maintaining the differentiated state of these cells. (aacrjournals.org)
  • Overall, both gene ontology and KEGG pathway analysis revealed enrichment of cell cycle activity in cells. (jhu.edu)
  • Here we report about the successful identification of an endogenous highly active gene promoter obtained from CHO cells which shows conditionally inducible gene expression at reduced temperature. (biomedcentral.com)
  • This novel CHO promoter permits conditionally high-level gene expression. (biomedcentral.com)
  • A successful example of a cell specific constitutive promoter represents that of the CHO-derived elongation factor-1 (CHEF-1) gene [ 8 ], where high expression levels were observed for several genes and different cell lines. (biomedcentral.com)
  • The unaltered apoptotic rate supported the proposition that the increase in cell number was a result of enhance cell cycle kinetics and cellular metabolism rather than increasing viability. (doaj.org)
  • Studies have demonstrated that silencing certain target genes involved in certain CHO cell apoptotic and metabolic pathways resulted in 40 to 60% improved cell viability as compared with untreated cells. (thefreedictionary.com)
  • Previously, we have reported that SPD inhibited the proliferation of MCF-7 human breast cancer cells by inducing apoptotic cell death, while having minimal effects on non-malignant cells. (nih.gov)
  • To confirm that the SPD-induced apoptotic cell death was due to the involvement of caspase-7, cells were also treated with SPD in the presence of the specific inhibitor of caspase-7, Ac-DEVD-CHO . (nih.gov)
  • Note: If cell viability is below 90%, troubleshoot conditions prior to continuing. (sigmaaldrich.com)
  • Addition of a mixture of amino acids was found to improve cell viability, which got altered by high glucose in the CHO-K1 cells. (biomedsearch.com)
  • Very high cell densities were achieved and could be stably maintained at high viability indicating of a healthy process suitable for instance for efficient cell banking. (diva-portal.org)
  • Also, cultured cells show diminished angiotensin II receptor binding with respect to time in culture and passage number. (springer.com)
  • To address these problems, we expressed the recombinant AT 1A receptor in CHO-K1 cells. (springer.com)
  • In AT 1A receptor transfected CHO-K1 cells, angiotensin II (10 −9 M) stimulated a rapid increase in cytosolic free calcium that was completely desensitized within 50 sec following receptor occupancy. (springer.com)
  • Receptor desensitization was also unaffected by inhibition or activation of protein kinase C. Thus, we have established a permanent, high-level transfectant of the AT 1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following exposure to physiological concentrations of agonist. (springer.com)
  • ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. (biovendor.com)
  • Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor ( J. Watson and J.V. (bio-medicine.org)
  • CHO cells expressing the h5-HT 1B receptor were diluted to a desired membrane protein concentration in 25 mM HEPES buffer containing 2.5 mM CaCl2 and 1 mM MgCl2, at pH 7.4. (bio-medicine.org)
  • Anthrax protective antigen interacts with a specific receptor on the surface of CHO-K1 cells. (asm.org)
  • Pretreatment of cells with a number of different proteases strongly inhibits PA binding, suggesting that the receptor may be at least partially proteinaceous. (asm.org)
  • Our results suggest that a cell surface protein(s) of 85 to 90 kDa is, or constitutes a portion of, a specific receptor for the PA. (asm.org)
  • For example, a very different set of expertise would be required to operate a microbial fermenter than to operate a CHO cell bioreactor. (thefreedictionary.com)
  • The increasing role and importance of cell culture in biophamaceutical manufacturing has led to considerable research and development (R&D) into bioreactor design and performance in recent years. (bioprocessintl.com)
  • As a result, a greater understanding of bioreactor fluid dynamics and critical physical parameters is now essential to maximize cell growth and productivity. (bioprocessintl.com)
  • Horizon licenses its CHO expression system to pharmaceutical, biotechnology, and biosimilar companies, as well as contract manufacturing organizations. (horizondiscovery.com)
  • Baser B, Spehr J, Bussow K, van den Heuvel J (2016) A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells. (springer.com)
  • Infecting cells with this virus at an MOI of over 2 x 105 particles per cell, we have demonstrated that this unique genomic locus can be successfully targeted with foreign DNA through homologous recombination in CHO cells, with a targeting frequency of 3.1% calculated in this instance. (dcu.ie)
  • The S100a6 (calcyclin) and its flanking regions were identified from a genomic CHO-K1 lambda-phage library. (biomedcentral.com)
  • Specifically, the higher scale model is a cell culture dynamics (CCDyn) model, which describes cell growth and the variation of the nutrient and product concentrations over time in the extracellular medium. (aiche.org)
  • These data highlight some of our results in applying RNAi to augment CHO cell growth by targeting both cell death and metabolic pathways that are detrimental to the optimal performance of cells grown in bioreactors. (thefreedictionary.com)
  • This application note compares cell growth and recombinant protein production of multiple CHO cell clones when cultured in GE HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements. (labonline.com.au)
  • Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. (creativebiomart.net)
  • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. (creativebiomart.net)
  • What is the market size in 2018 and the growth rate of the Global Recombinant Hamster Ovary Cell (CHO) Hepatitis B Vaccine Market? (pharmiweb.com)
  • We followed the same logic with our CHO cell culture, with an objective function of cell growth. (bioprocessintl.com)
  • Despite let-7 having a recognised role in deregulated cell growth no improvement was observed in stable, sponge-transfected clones. (dcu.ie)
  • In the second approach, we targeted a previously verified miRNA for improved CHO cell growth and productivity i.e. miR-7, using a bacterial genome-editing tool, CRISPR-Cas9. (dcu.ie)
  • CHOMACS CD medium is a complete chemically-defined, animal component-free, protein-free, and ready-to-use media developed for long term, high performance growth and protein yield of CHO cells. (miltenyibiotec.com)
  • The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. (innoget.com)
  • In the presence of S9-mix, cell growth was 55% of the solvent control at 1500 g/mL and 0% at 5000 g/mL. (epa.gov)
  • After 44 hours exposure, cell growth remained above 50% of the solvent control value at concentrations up to and including 500 g/mL and was reduced to 42%, 18% and 0% at 1000, 1500 and 3000 g/mL, respectively. (epa.gov)
  • In the presence of S9-mix (6 hour exposure), cell growth remained above 50% of the solvent control value at all concentrations except 3000 g/mL where growth was 3% of the solvent control. (epa.gov)
  • HyCell™ CHO establishes a new benchmark for cell growth and productivity. (technologynetworks.com)
  • Its versatility allows quick adaptation and supports exceptional growth, high cell density and high productivity. (technologynetworks.com)
  • This property is of particular advantage for a process with reduced expression during initial cell growth followed by the production phase at low temperature with a boost in expression. (biomedcentral.com)
  • It is the first-in-class of a kind of drug called a "HER dimerization inhibitor" - it inhibits the dimerization of HER2 with other HER receptors, which prevents them from signalling in ways that promote cell growth and proliferation. (wikipedia.org)
  • The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and plasticity in the context of genetic alterations. (aiche.org)
  • A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros, Oregon Green 488, and Hoechst 33258. (microscopyu.com)
  • Aseptically remove a small volume of cell culture sample from the flask and count by trypan blue exclusion using a hemocytometer or an automated cell counter. (sigmaaldrich.com)
  • Passage cells by repeating the above steps at least twice weekly, and expand culture volume as necessary. (sigmaaldrich.com)
  • It is also known that the availability of the NSs is affected by process conditions including cell culture mode, temperature, dissolved oxygen tension, nutrient availability and addition of precursor molecules [1, (aiche.org)
  • No cell culture is required prior to testing, for these frozen cells. (perkinelmer.com)
  • The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. (perkinelmer.com)
  • That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. (perkinelmer.com)
  • Hi all, i usually culture my cho cells in f12 medium with 10% fcs. (protocol-online.org)
  • but now i would like to culture the cho cells in serum free media for 72-96h. (protocol-online.org)
  • These cells are robust in culture and can produce a variety of recombinant glycoproteins at high levels in large scales. (labonline.com.au)
  • In the drug development timeline, cell culture medium and any associated supplements should be introduced and tested right from the R&D phase. (labonline.com.au)
  • Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. (creativebiomart.net)
  • The cells will re-attach to the surface over a period of several days in culture at 37°C. (creativebiomart.net)
  • 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. (creativebiomart.net)
  • Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. (creativebiomart.net)
  • Crotalus durissus terrificus venom and its components can affect a variety of cell types, including macrophages (9), neutrophils (34), mast cells (8), platelets (7) and cells in culture (14), as well as the function of organs such as the heart (19) and the kidney (22). (scielo.br)
  • Venom stock solutions (2.5mg/ml) were prepared in phosphate buffered saline (0.1M sodium phosphate, 0.15M NaCl), pH 7.2, sterilized using a 0.22µm filter (Millipore) and kept at 4°C. Crude venom solutions were diluted in cell culture medium before use. (scielo.br)
  • Our work in optimization of animal-cell culture is a hybrid approach between using basal media and very complete, chemically defined, and serum-free media. (bioprocessintl.com)
  • Sterility of cell culture media is an important concern in biotherapeutic processing. (uwaterloo.ca)
  • In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. (uwaterloo.ca)
  • This makes UV irradiation attractive for use in sterilization of cell culture media. (uwaterloo.ca)
  • The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. (uwaterloo.ca)
  • Here, we describe genetic approaches to improve CHO cell culture longevity, with a view to increasing overall process yield via the manipulation of two miRNAs: Let-7a and miR-7. (dcu.ie)
  • In the first approach, we used a Let-7 sponge decoy vector to deplete endogenous Let-7 levels with a view to increasing culture longevity and productivity of CHO-K1 SEAP expressing cells. (dcu.ie)
  • The design and manufacture of single-use bioreactors is another major area of R&D. The objectives have been to achieve and (when possible) improve fluid dynamics and cell culture performance of stainless steel systems but in a single-use, presterilized format. (bioprocessintl.com)
  • In addition, the effects of cell culture conditions on protein production in transiently transfected cells are illustrated. (selectscience.net)
  • The method was successfully applied to analyze the extracts from CHO cells under two different culture conditions. (springer.com)
  • Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. (semanticscholar.org)
  • In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. (physiciansweekly.com)
  • For more information about Thermo Scientific HyCell CHO cell-culture medium, to request a sample or to learn more about Thermo Scientific Cell Culture and Bioprocessing technologies, please visit www.thermoscientific.com/bioprocessing. (technologynetworks.com)
  • ProBioGen's royalty-free, fee-for service-based CHO platform is based on almost 20 years of experience in mammalian cell culture. (probiogen.de)
  • Site-specific integration of transgenes into CHO hot spots is an ideal strategy to overcome these challenges since it can generate isogenic cell lines with consistent productivity and stability. (springer.com)
  • Gao Y et al (2016) Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess. (springer.com)
  • Application of C-13 flux analysis to identify high-productivity CHO metabolic phenotypes. (springer.com)
  • Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. (semanticscholar.org)
  • phase and to the uncoupling of cell size from cell proliferation. (doaj.org)
  • The purpose of this study was to determine if PbTxs could induce chromosomal aberrations and inhibit cellular proliferation in CHO-K1-BH4 cells and if so could the damage be negated or reduced by the PbTx antagonist brevenal. (uncg.edu)
  • Results from the inhibition of cellular proliferation assays demonstrated that PbTxs inhibit the ability of CHOK1- BH4 cells to proliferate an effect which can be reduced with brevenal. (uncg.edu)
  • We demonstrate that CRISPR-Cas9 can be successfully used to target miRNA loci in the CHO genome but that functional knockout may be more difficult compared to protein coding genes. (dcu.ie)
  • However, microarray studies also implicated additional transcriptional targets in GSI-I-dependent cell death, including genes in the unfolded protein response, nuclear factor-κB and p53 pathways. (pubmedcentralcanada.ca)
  • Based on CHO microarray expression data abundantly transcribed genes were selected as potential promoter candidates. (biomedcentral.com)
  • CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. (thermofisher.com)
  • Therefore, this stimulated the interest of our research group to manipulate these miRNAs in CHO cells with a view to positively impact bioprocess-relevant CHO cell phenotypes. (dcu.ie)
  • The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. (rupress.org)
  • Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25. (rupress.org)
  • Incorporating this technology into our biomanufacturing processes enhances our ability to efficiently generate high quality cell lines. (horizondiscovery.com)
  • These results suggest the presence of an inducible DNA pathway in CHO cells that results from an enhancement of TCR or a mechanism that involves the TCR pathway. (portlandpress.com)
  • Finally, titers of soluble TNFαR are shown to increase for at least one week and are sustained for up to two weeks following electroporation of CHO cells with a sTNFαR expression plasmid. (selectscience.net)
  • I have to start a lot of infection experiments on these cell lines and i need to be sure they are worth the effort! (protocol-online.org)
  • The cells in F-12K1 serum-free medium were exposed to normal (7 mM) and high glucose (12, 17 and 27 mM) in the presence and absence of amino acids mixture (AAM) in varying concentration (2.5, 5 and 10 mM). (biomedsearch.com)
  • Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. (creativebiomart.net)
  • PerkinElmer's validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. (perkinelmer.com)
  • Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. (perkinelmer.com)
  • Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. (perkinelmer.com)
  • FroZen, -irradiated cells, are a well established product, that can be ordered as a consumable, and readily used to perform Aequorin functional assays (AequoZen) or cAMP assays (cAMPZen). (perkinelmer.com)
  • Some of our cAMPZen™ FroZen Cells may be restricted for sale in specified countries. (perkinelmer.co.uk)
  • The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. (scielo.br)
  • The aim of the present work was, therefore, to determine the effect of crotalid venom on CHO-K1 cells, particularly its action on actin filaments, endoplasmic reticulum and nucleus. (scielo.br)
  • These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance. (perkinelmer.com)
  • Mouse CD8A & CD8B Heterodimer HEK293 Cell Lysate (WB positive control) [ Ӧ? (bioon.com.cn)
  • Human IL-23 (IL12B & IL23A Heterodimer) HEK293 Cell Lysate (WB positive control) [ Ӧ? (bioon.com.cn)
  • Stable cell lines were generated successfully after a single round of selection. (springer.com)
  • The Freedom™ CHO-S™ Kit platform enables end-users the cost- and time-efficient creation of stable cell lines for research and commercial purposes. (probiogen.de)