Cho cells. Medical search

CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Established cell cultures that have the potential to propagate indefinitely.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The rate dynamics in chemical or physical systems.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).

Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.

Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.

A narcotic antagonist similar in action to NALOXONE. It is used to remobilize animals after ETORPHINE neuroleptanalgesia and is considered a specific antagonist to etorphine.

Adherence of cells to surfaces or to other cells.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.

An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3.

An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

Glycoproteins found on the membrane or surface of cells.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

A cell line derived from cultured tumor cells.

Aquatic vertebrate sensory system in fish and amphibians. It is composed of sense organs (canal organs and pit organs) containing neuromasts (MECHANORECEPTORS) that detect water displacement caused by moving objects.

Studies in which variables relating to an individual or group of individuals are assessed over a period of time.

A peptide of about 22-amino acids isolated from the DUODENUM. At low pH it inhibits gastric motor activity, whereas at high pH it has a stimulating effect.

Cell surface proteins that bind gastrointestinal hormones with high affinity and trigger intracellular changes influencing the behavior of cells. Most gastrointestinal hormones also act as neurotransmitters so these receptors are also present in the central and peripheral nervous systems.

Cell surface receptors that bind specific neuropeptides with high affinity and trigger intracellular changes influencing the behavior of cells. Many neuropeptides are also hormones outside of the nervous system.

A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.

Drugs used for their effects on the gastrointestinal system, as to control gastric acidity, regulate gastrointestinal motility and water flow, and improve digestion.

A substituted benzamide used for its prokinetic properties. It is used in the management of gastroesophageal reflux disease, functional dyspepsia, and other disorders associated with impaired gastrointestinal motility. (Martindale The Extra Pharmacopoeia, 31st ed)

The region between the sharp indentation at the lower third of the STOMACH (incisura angularis) and the junction of the PYLORUS with the DUODENUM. Pyloric antral glands contain mucus-secreting cells and gastrin-secreting endocrine cells (G CELLS).

A plant genus of the family ASCLEPIADACEAE. Members contain steroidal glycosides and cytotoxic phenanthroindolizidine N-oxide alkaloids.

Unsaturated derivatives of PREGNANES.

The study of the physical and chemical properties of a drug and its dosage form as related to the onset, duration, and intensity of its action.

Proteins prepared by recombinant DNA technology.

Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

In this study the direct involvement of cadherins in adhesion-mediated growth inhibition was investigated. It is shown here that overexpression of N-cadherin in CHO cells significantly suppresses their growth rate. Interaction of these cells and two additional fibroblastic lines with synthetic beads coated with N-cadherin ligands (recombinant N-cadherin ectodomain or specific antibodies) leads to growth arrest at the G1 phase of the cell cycle. The cadherin-reactive beads inhibit the entry into S phase and the reduction in the levels of cyclin-dependent kinase (cdk) inhibitors p21 and p27, following serum-stimulation of starved cells. In exponentially growing cells these beads induce G1 arrest accompanied by elevation in p27 only. We propose that cadherin-mediated signaling is involved in contact inhibition of growth by inducing cell cycle arrest at the G1 phase and elevation of p27 levels. (+info)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

The DNA repair protein apurinic endonuclease (APE/Ref-1) exerts several physiological functions such as cleavage of apurinic/apyrimidinic sites and redox regulation of the transcription factor AP-1, whose activation is part of the cellular response to DNA damaging treatments. Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towards AP-1, thus promoting DNA binding activity of AP-1. Inhibition of CKII mediated phosphorylation of APE/Ref-1 blocked mutagen-stimulated increase in AP-1 binding. It also abrogated the induction of c-Jun protein and rendered cells more sensitive to induced DNA damage. Thus, phosphorylation of APE/Ref-1 appears to be involved in regulating the different physiological activities of the enzyme. CKII mediated phosphorylation of APE/Ref-1 and concomitant increase in AP-1 binding activity appears to be a novel mechanism of cellular stress response, forcing transcription of AP-1 target gene(s) the product(s) of which may exert protective function. (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located. (+info)

Proteoglycan involvement in polyamine uptake. (4/15188)

We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake considerably, whereas chondroitin sulphate lyase had a limited effect. Inhibition of polyamine biosynthesis with alpha-difluoromethylornithine resulted in an increase of cell-associated heparan sulphate proteoglycans exhibiting higher affinity for spermine. The data indicate a specific role for heparan sulphate proteoglycans in the uptake of spermine by fibroblasts. Spermine uptake by pgsD-677, a mutant Chinese hamster ovary cell defective in heparan sulphate biosynthesis, was only moderately reduced (20%) compared with wild-type cells. Treatment of mutant cells with the above-mentioned xyloside resulted in a greater reduction of endogenous proteoglycan production as well as a higher inhibition of spermine uptake than in wild-type cells. Moreover, treatment with chondroitin sulphate lyase resulted in a selective inhibition of uptake in mutant cells, indicating a role for chondroitin/dermatan sulphate proteoglycans in the uptake of spermine by these cells. Fibroblasts, made growth-dependent on exogenous spermine by alpha-difluoromethylornithine treatment, were growth-inhibited by heparan sulphate or beta-D-xyloside, which might have future therapeutical implications. (+info)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Serum-free culture filtrates of six Candida species and Saccharomyces cerevisiae were found to contain chemoattractants for human polymorphonuclear leukocytes (PMNs) and a mouse macrophage-like cell line, J774. The chemotactic factors differed for the PMN and J774 cells, however, in terms of heat stability, kinetics of liberation by the yeast cells, and divalent cation requirements for production. The chemoattractant in Candida albicans culture filtrates appeared to act through the formyl peptide receptor (FPR) of PMNs, since it was found to induce chemotaxis of Chinese hamster ovary (CHO) cells that were expressing the human FPR but did not induce chemotaxis of wild-type CHO cells. The C. albicans culture filtrates also induced migration of PMNs across confluent monolayers of a human gastrointestinal epithelial cell line, T84; migration occurred in the basolateral-to-apical direction but not the reverse direction, unless the epithelial tight junctions were disrupted. J774 cells did not migrate toward the formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albicans culture filtrate was not inhibited by an FPR antagonist (t-butoxycarbonyl-Met-Leu-Phe), suggesting that a different receptor mediated J774 cell chemotaxis. In conclusion, we have identified a receptor by which a non-serum-dependent chemotactic factor (NSCF) produced by C. albicans induced chemotaxis of PMNs. Additionally, we have shown that NSCF was active across epithelial monolayers. These findings suggest that NSCFs produced by C. albicans and other yeast species may influence host-pathogen interactions at the gastrointestinal tract mucosal surface by inducing phagocytic-cell infiltration. (+info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology. (+info)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen. (+info)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein. (+info)

CHO cells[edit]. DHFR lacking CHO cells are the most commonly used cell line for the production of recombinant proteins. These ... which are important for cell proliferation and cell growth.[14] DHFR plays a central role in the synthesis of nucleic acid ... regulation of transcription involved in G1/S transition of mitotic cell cycle. • tetrahydrofolate metabolic process. • ... DHFR is responsible for the levels of tetrahydrofolate in a cell, and the inhibition of DHFR can limit the growth and ...

https://en.wikipedia.org/wiki/Dihydrofolate_reductase

Efti is manufactured in CHO cells. Immutep worked with Henogen as the contracted manufacturing organization to provide efti for ... Activated T cells. The eight patients experienced sustained CD8+ T-cell activation (as measured by percentage of CD8+ T cells ... Increase in relevant cell numbers. There was a sustained increase in the number of monocytes, NK cells, and activated CD8+ T ... Cell Genesys' lead product, GVAX, consisted of whole tumor cells genetically modified to secrete GM-CSF. When mice that had ...

https://en.wikipedia.org/wiki/Eftilagimod_alpha

... it is manufactured in CHO cells. As of 2016 it was in phase III studies for induction and maintenance therapy in people with ... Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ... "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ...

https://en.wikipedia.org/wiki/Etrolizumab

Gieseler, A (2013). "Cell Membrane Toponomics". "Cell Membrane Toponomics" in Dubitzky, Wolkenhauer, Cho, Yokota. Encyclopedia ... "3D all-organelle real time visualization of a single cell" "Visualizing the protein-DNA network code inside the cell nucleus" ... Detection of 2000 Cell Surface Protein Clusters in a Single Tissue Section and Cell Type Specific Annotation by Using a Three ... detection of CD8+ CD18+ cells and CD8+ CD103+ cells by multi-epitope imaging". Clinical and Experimental Immunology. 112 (1): ...

https://en.wikipedia.org/wiki/Imaging_cycler_microscopy

It is recombinantly manufactured in Chinese hamster ovary (CHO) cells. Pembrolizumab was invented by scientists Gregory Carven ... the PD-1 receptor on activated T-cells binds to ligands PD-L1 or PD-L2 on other cells, deactivating a potential T-cell-mediated ... It works by targeting the cellular pathway of proteins found on the body's immune cells and some cancer cells, known as PD-1/PD ... Results of a Phase II clinical trial in Merkel-cell carcinoma were reported in The New England Journal of Medicine in June 2016 ...

https://en.wikipedia.org/wiki/Pembrolizumab

"Phase I Trial of a Recombinant COVID-19 Vaccine (CHO Cell)". clinicaltrials.gov. United States National Library of Medicine. ... "Immunogenicity and Safety of Recombinant COVID-19 Vaccine (CHO Cells)". clinicaltrials.gov. United States National Library of ...

https://en.wikipedia.org/wiki/Zhongyianke_Biotech–Liaoning_Maokangyuan_Biotech_COVID-19_vaccine

... animal cells (i.e. Chinese hamster ovary cells, or CHO cells), and plant cells. Lysosomal storage diseases are fatal group of ... These cells have the ability to mature into the many cell types that comprise blood, including red blood cells, platelets, and ... Hematopoietic stem cell (HSC) transplantation is another treatment for lysosomal storage diseases. HSCs are derived from bone- ... Once inside the body the vector introduces the therapeutic gene into host cells, and the protein encoded by the newly inserted ...

https://en.wikipedia.org/wiki/Enzyme_replacement_therapy

"Phleomycin resistance as a dominant selectable marker in CHO cells". Somatic Cell and Molecular Genetics. 14 (3): 243-52. doi: ... It causes cell death by intercalating into DNA and inducing double stranded breaks of the DNA. Zeocin is blue in colour due to ... When zeocin enters a cell, the Cu2+ is reduced to Cu+ and then removed, and zeocin becomes activated and can then bind DNA. ... It is a broad-spectrum antibiotic that is effective against most bacteria, filamentous fungi, yeast, plant, and animal cells. ...

https://en.wikipedia.org/wiki/Zeocin

Thielemans L, Depoortere I, Vanden Broeck J, Peeters TL (2002). "The motilin pharmacophore in CHO cells expressing the human ... 2001). "Demonstration of a functional motilin receptor in TE671 cells from human cerebellum". Brain Res. 895 (1-2): 119-28. doi ...

https://en.wikipedia.org/wiki/Motilin_receptor

CHO) cells were isolated, and this gene restored UV resistance to cells of complementation group 1. The human ERCC1 gene ... Wood RD, Burki HJ, Hughes M, Poley A (February 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell ... Hayashi T, Takao M, Tanaka K, Yasui A (June 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... Mammalian cells with mutant ERCC1-XPF are moderately more sensitive than normal cells to agents (such as ionizing radiation) ...

https://en.wikipedia.org/wiki/ERCC1

Hayashi T, Takao M, Tanaka K, Yasui A (Jun 1998). "ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines". ... DNA repair protein complementing XP-A cells is a protein that in humans is encoded by the XPA gene. Nucleotide excision repair ... "Analysis of 133 meioses places the genes for nevoid basal cell carcinoma (Gorlin) syndrome and Fanconi anemia group C in a 2.6- ...

https://en.wikipedia.org/wiki/XPA

Yokomizo T, Noiri E, Izumi T, Shimizu T (2003). "In vivo chemotaxis using CHO cells expressing human leukotriene B4 receptor". ... Kato K, Yokomizo T, Izumi T, Shimizu T (2000). "Cell-specific transcriptional regulation of human leukotriene B(4) receptor ... "Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells". J Biol Chem. 271 (31): 18363-7. doi:10.1074/jbc ... "Leukotriene B4 receptor antagonist LY293111 inhibits proliferation and induces apoptosis in human pancreatic cancer cells". ...

https://en.wikipedia.org/wiki/Leukotriene_B4_receptor_1

The clastogen-suppressing effects of Tochu tea in CHO cells and mice. „Mutation research". 388 (1), s. 7-20, 1997. DOI: 10.1016 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line.. „Cancer Research". 61 (15), s. 5749 ... Scopoletin suppresses pro-inflammatory cytokines and PGE2 from LPS-stimulated cell line, RAW 264.7 cells.. „Fitoterapia". 75 (3 ... Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor kappaB and caspase-3 ...

https://pl.wikipedia.org/wiki/Morwa_indyjska

"The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells". Proc Natl Acad Sci U S A. ... "Role of Fanconi DNA repair pathway in neural stem cell homeostasis". Cell Cycle. 7 (13): 1911-5. doi:10.4161/cc.7.13.6235. PMID ... Cell. 7 (2): 249-62. doi:10.1016/s1097-2765(01)00173-3. PMID 11239454. Yang Y, Kuang Y, Montes De Oca R, Hays T, Moreau L, Lu N ... Cell. Biol. 19 (7): 4866-73. doi:10.1128/mcb.19.7.4866. PMC 84285 . PMID 10373536. Park SJ, Ciccone SL, Beck BD, Hwang B, Freie ...

https://en.wikipedia.org/wiki/FANCG

Wood RD, Burki HJ, Hughes M, Poley A (Feb 1983). "Radiation-induced lethality and mutation in a repair-deficient CHO cell line ... If the stem cells at the base of the crypt express ERCC4 (XPF), generally all several thousand cells of the crypt will also ... Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV ... Nuclei of cells in the lamina propria, cells which are below and surround the epithelial crypts, largely show hematoxylin blue- ...

https://en.wikipedia.org/wiki/ERCC4

Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... "Unconventional mechanisms of protein transport to the cell surface of eukaryotic cells". Annual Review of Cell and ... I. Detection in the microsomal membrane of a receptor for the signal recognition particle". The Journal of Cell Biology. 95 (2 ... Signal peptides function to prompt a cell to translocate the protein, usually to the cellular membrane. In prokaryotes, signal ...

https://en.wikipedia.org/wiki/Signal_peptide

The DNA was damaged in CHO cells as well as in mice. In mice (given 20 mg nivalenol /kg bw orally or 3.7 mg /kg bw ip) the DNA ... CHO) cells by slightly increased frequencies of chromosomal aberrations and sister chromatid exchange. ... NF-κB is a transcription factor that can be found in almost all human cells, and regulates the expression of its target genes ... Again, this effect is reduced by NF-κB inhibition which shows, that nivalenol and NF-κB interact to influence the cell. It was ...

https://en.wikipedia.org/wiki/Nivalenol

The clastogen-suppressing effects of Tochu tea in CHO cells and mice". Mutation Research. 388 (1): 7-20. doi:10.1016/s1383-5718 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line". Cancer Research. 61 (15): 5749-56. ...

https://en.wikipedia.org/wiki/Deacetylasperulosidic_acid

Kober L, Zehe C, Bode J (April 2013). "Optimized signal peptides for the development of high expressing CHO cell lines". ... this gives cells the flexibility to adapt to a variable environment, external signals, damage to the cell, and other stimuli. ... Cell. 172 (4): 650-665. doi:10.1016/j.cell.2018.01.029. PMID 29425488. Grossman SR, Engreitz J, Ray JP, Nguyen TH, Hacohen N, ... Cell. 171 (7): 1573-1588.e28. doi:10.1016/j.cell.2017.11.008. PMC 5785279. PMID 29224777. Lambert SA, Jolma A, Campitelli LF, ...

https://en.wikipedia.org/wiki/Gene_expression

"Evidence obtained by segregation analysis for functional hemizygosity at the Emtr locus in CHO cells". Cell. 14: 1007-1013. doi ... In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ... A chromosome in a diploid organism is hemizygous when only one copy is present.[2] The cell or organism is called a hemizygote ...

https://en.wikipedia.org/wiki/Heterozygote

Barron N, Kumar N, Sanchez N, Doolan P, Clarke C, Meleady P, O'Sullivan F, Clynes M (January 2011). "Engineering CHO cell ... "Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin". ... a methylation-sensitive microRNA is involved in cell proliferation and invasion in human colorectal cancer". Carcinogenesis. 32 ... cellular motility and oxidative phosphorylation in neural precursors derived from human umbilical cord mesenchymal stem cells ...

https://en.wikipedia.org/wiki/Mir-345_microRNA_precursor_family

Skalski M, Coppolino MG (Oct 2005). "SNARE-mediated trafficking of alpha5beta1 integrin is required for spreading in CHO cells ... Bves demonstrates dynamic localization, dependent upon cell-cell junction formation. Prior to cell-cell contact, Bves is ... Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho ... Grossly, cell motility and cell adhesion are impaired. Only recently have the molecular mechanisms underlying the function of ...

https://en.wikipedia.org/wiki/Blood_vessel_epicardial_substance

"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ... "Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells". Mol. Pharmacol. 35 (4): 469-76. ... Acetylcholine (often abbreviated ACh) is a neurotransmitter whose receptors are proteins found in synapses and other cell ... "Decreased binding affinity of olanzapine and clozapine for human muscarinic receptors in intact clonal cells in physiological ...

https://en.wikipedia.org/wiki/Muscarinic_antagonist

Barron N, Kumar N, Sanchez N, Doolan P, Clarke C, Meleady P, O'Sullivan F, Clynes M (January 2011). "Engineering CHO cell ... "Differences in osteoblast miRNA induced by cell binding domain of collagen and silicate-based synthetic bone". Journal of ... "miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC)". British Journal of ... "Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells". PLOS ONE. 4 (5): ...

https://en.wikipedia.org/wiki/Mir-489_microRNA_precursor_family

2015-03-04). "Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells". mAbs. 7 (2): 403-12. ... Therefore, up to 50% of F2A-linked proteins can remain in the cell as a fusion protein, which might cause some unpredictable ... 2011-04-29). Thiel V (ed.). "High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, ... April 2019). "Fusion of Reprogramming Factors Alters the Trajectory of Somatic Lineage Conversion". Cell Reports. 27 (1): 30-39 ...

https://en.wikipedia.org/wiki/2A_self-cleaving_peptides

This was followed by production of clinical grade alpha-glucosidase in Chinese hamster ovary (CHO) cells and in the milk of ... Genzyme also had its own product (Myozyme) grown in CHO cells under development. In November 2001, Genzyme chief executive ... muscle cells) or in white blood cells. The choice of sample depends on the facilities available at the diagnostic laboratory. ... Administration of the enzyme lead to its uptake by the liver and not the muscle cells where it is needed. In the early 1990s ...

https://en.wikipedia.org/wiki/Glycogen_storage_disease_type_II

"Metastin suppresses the motility and growth of CHO cells transfected with its receptor". Biochemical and Biophysical Research ... "Transcriptional expression of genes involved in cell invasion and migration by normal and tumoral trophoblast cells". The ... Journal of Cell Science. 117 (Pt 8): 1319-28. doi:10.1242/jcs.00971. PMID 15020672. "KiSS1-Derived Peptide Receptors". IUPHAR ... "Metastin receptor is overexpressed in papillary thyroid cancer and activates MAP kinase in thyroid cancer cells". The Journal ...

https://en.wikipedia.org/wiki/KiSS1-derived_peptide_receptor

2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry. 43 ( ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3 PDB: 3NK4 Jovine L, Qi H, ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3, 3NK4 Bansal P, Chakrabarti K ... Cell Biol. 4 (6): 457-61. doi:10.1038/ncb802. PMID 12021773. S2CID 11575790. Jovine L, Qi H, Williams Z, Litscher ES, Wassarman ...

https://en.wikipedia.org/wiki/ZP3

"Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein". Cell. 87 (3): 415-26. doi: ... a possible trigger for degradation of HMG CoA reductase and crystalloid endoplasmic reticulum in UT-1 cells". Cell. 36 (4): 835 ... Sakai J, Duncan EA, Rawson RB, Hua X, Brown MS, Goldstein JL (Jun 1996). "Sterol-regulated release of SREBP-2 from cell ... Nov 1984). "The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA". Cell. 39 (1): 27-38. doi: ...

https://en.wikipedia.org/wiki/Michael_Stuart_Brown

He developed the CHO cell line from Chinese hamster ovarian cells for this work. These cells are still widely utilized in the ... Puck was an early pioneer of "somatic cell genetics" and single-cell plating ( i.e. "cloning" .) This work allowed the genetics ... Chromosomal constitution of cells in tissue culture". Journal of Experimental Medicine. 108 (2): 259-268. doi:10.1084/jem.108.2 ... Puck with the assistance of Philip I. Marcus, successfully cloned a HeLa cell in 1955. Puck made many basic discoveries in ...

https://en.wikipedia.org/wiki/Theodore_Puck

... a type 2 diabetic will have lost about half of their beta cells.[52] Fatty acids in the beta cells activate FOXO1, resulting in ... Cho Y, Nadeau-Fredette AC, Burke M, Faruque L, Lloyd A, Ahmad N, Liu Y, Tiv S, Wiebe N, Strippoli GF (July 2016). "Comparison ... Type 2 diabetes is due to insufficient insulin production from beta cells in the setting of insulin resistance.[13] Insulin ... In the early stages of insulin resistance, the mass of beta cells expands, increasing the output of insulin to compensate for ...

https://en.wikipedia.org/wiki/Type_2_diabetes_mellitus

CHO, Z. H., ERIKSSON L., and CHAN J.K., ``A circular ring transverse axial positron camera in Reconstruction Tomography in ... This tracer is a glucose analog that is taken up by glucose-using cells and phosphorylated by hexokinase (whose mitochondrial ... Although many investigators took this approach, James Robertson[66] and Zang-Hee Cho[67] were the first to propose a ring ... This means that FDG is trapped in any cell that takes it up until it decays, since phosphorylated sugars, due to their ionic ...

https://en.m.wikipedia.org/wiki/PET_scan

... the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell ... "Cell. 136 (2): 272-83. doi:10.1016/j.cell.2008.11.047. PMC 2859625. PMID 19167329.. ... "J. Cell Biol. 161 (4): 715-25. doi:10.1083/jcb.200301043. PMC 2199356. PMID 12756234.. ...

https://en.wikipedia.org/wiki/Oligosaccharyltransferase

2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry. 43 ( ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237.. PDB: 3NK3 PDB: 3NK4 ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237.. PDB: 3NK3, 3NK4 ... Cell. Biol. 22 (9): 3111-20. doi:10.1128/MCB.22.9.3111-3120.2002. PMC 133755. PMID 11940668.. ...

https://en.m.wikipedia.org/wiki/ZP3

Zhou T, Zhang J, Carter R, Kimberly R (2003). "BLyS and B cell autoimmunity.". Curr. Dir. Autoimmun. 6: 21-37. PMID 12408045. ... Wu Y, Bressette D, Carrell JA, Kaufman T, Feng P, Taylor K, Gan Y, Cho YH, Garcia AD, Gollatz E, Dimke D, LaFleur D, Migone TS ... Brink R (2007). "Regulation of B cell self-tolerance by BAFF.". Semin. Immunol. 18 (5): 276-83. PMID 16916609. doi:10.1016/j. ... Mackay F, Leung H (2007). "The role of the BAFF/APRIL system on T cell function.". Semin. Immunol. 18 (5): 284-9. PMID 16931039 ...

https://sh.wikipedia.org/wiki/Faktor_aktivacije_B-ćelija

These M cells then alert the underlying B cells and T cells in the tonsil that a pathogen is present and an immune response is ... Wang, JH; Chung, YS; Cho, YW; Kim, DY; Yi, JS; Bae, JS; Shim, MJ (April 2010). "Palatine tonsil size in obese, overweight, and ... The tonsils have on their surface specialized antigen capture cells called M cells that allow for the uptake of antigens ... "Tonsils Make T-Cells, Too, Ohio State Study Shows". Ohio State University. Ohio State University, Comprehensive Cancer Center. ...

https://en.wikipedia.org/wiki/Tonsil

Cho WC, Nam OH, Kim MS, Lee HS, Choi SC (May 2018). "A retrospective study of traumatic dental injuries in primary dentition: ... as this will damage the delicate cells that make up the tooth's interior. ...

https://en.wikipedia.org/wiki/Dental_trauma

"cho" በሦስት መክተብ ኣያፈጥንም። ተጨማሪ ምሳሌ ከኣስፈለገ ኣንድ የኢትዮጵያ ኦርቶዶክስ ቤተ ክርስቲያን ዓማርኛ በላቲን ፊደል እንዲጻፍና በኣጻጻፉም በግዕዝ ፊደል ምትክ በላቲን ፊደል ጽፎ ያቀረበው ... 1,306] Cell Phone Typing in Amharic የእጅ ስልክ. *[1,307] የእጅ ስልክ. *[1,308] Ethiopian eResources ...

https://am.wikipedia.org/wiki/ኣበራ_ሞላ

Cho DY, Frey RA, Guffy MM, Leipold HW (November 1975). "Hypervitaminosis A in the dog". American Journal of Veterinary Research ... Wu J, Zern MA (2000). "Hepatic stellate cells: a target for the treatment of liver fibrosis". Journal of Gastroenterology. 35 ( ... Vitamin K prevents hypoprothrombinemia in rats and can sometimes control the increase in plasma/cell ratios of vitamin A.[49] ... Levine PH, Delgado Y, Theise ND, West AB (February 2003). "Stellate-cell lipidosis in liver biopsy specimens. Recognition and ...

https://en.m.wikipedia.org/wiki/Hypervitaminosis_A

cell nucleus. • kinetochore. • centrosome. • rough endoplasmic reticulum. • dendritic shaft. • aggresome. • cell surface. • ... Cho S, Lu M, He X, Ee PL, Bhat U, Schneider E, Miele L, Beck WT (December 2011). "Notch1 regulates the expression of the ... cell cortex. • integral component of membrane. • azurophil granule membrane. • Z disc. • neuronal cell body. • perinuclear ... cell-cell adhesion. • cellular response to amyloid-beta. • negative regulation of core promoter binding. • negative regulation ...

https://en.wikipedia.org/wiki/PSEN1

Kim JE, Cho BK, Cho DH, Park HJ (July 2013). "Expression of hypothalamic-pituitary-adrenal axis in common skin diseases: ... There, CRH and vasopressin act synergistically to stimulate the secretion of stored ACTH from corticotrope cells. ACTH is ... in immune cells, such as monocytes and neutrophils [8][9][11][12] ...

https://en.wikipedia.org/wiki/Hypothalamic-pituitary-adrenal_axis

Kim, Jin-Ho; Kim, Hyo-Sop; Lee, Jae-Hyeok; Choi, Sung-Wook; Cho, Yong-Jin; Kim, Jae-Ho (2009-12-01). "Hexagonally Close Packed ... to improve cell adhesion or study the properties of biofilms. An example of Langmuir-Blodgett troughs' utility in ...

https://en.wikipedia.org/wiki/Langmuir-Blodgett_trough

Am J Respir Cell Mol Biol. 45:88-94. Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... Am J Respir Cell Mol Biol. 41:661-70 Kang HR, Cho SJ, Lee CG, Homer RJ, Elias JA. (2007) Transforming growth factor (TGF)-beta1 ... 57:51-9. Tang PS, Mura M, Seth R, Liu M. (2008) Acute lung injury and cell death: how many ways can cells die? Am J Physiol 294 ... There are two types of alveolar epithelial cells - Type 1 pneumocytes represent 90% of the cell surface area, and are easily ...

https://en.wikipedia.org/wiki/Acute_inhalation_injury

Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... Yi, Eun Hee; Lee, Chang Seok; Lee, Jin-Ku; Lee, Young Ju; Shin, Min Kyung; Cho, Chung-Hyun; Kang, Keon Wook; Lee, Jung Weon; ... These T cells can then go on to perform effector functions such as macrophage activation, B cell activation, and cell-mediated ... When interleukin-1 is produced in response to external stimuli, it can bind to cell-surface receptors on the same cell that ...

https://en.wikipedia.org/wiki/Autocrine_signalling

Cho-Vega JH, Tsavachidis S, Do KA, Nakagawa J, Medeiros LJ, McDonnell TJ (2007). "Dicarbonyl/L-xylulose reductase: a potential ... Cell. Mol. Life Sci. 66 (9): 1570-9. doi:10.1007/s00018-009-9065-y. PMID 19337691.. ...

https://en.wikipedia.org/wiki/L-xylulose_reductase

Agonist-induced internalization and desensitization of the human nociceptin receptor expressed in CHO cells.". Cell. Mol. Life ... receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines.". Brain Res. Mol. Brain Res. 32 (2): ...

https://sr.wikipedia.org/sr-el/Nociceptinski_receptor

Place cells are also found in the hippocampus. The parietal cortex encodes spatial information using an egocentric frame of ... Cho, Y. H.; Kesner, R. P. (1996). "Involvement of entorhinal cortex or parietal cortex in long-term spatial discrimination ... Brun, V. H.; Otnaess, M. K.; Molden, S.; Steffenach, H.; Witter, M. P.; Moser, M.; Moser, E. I. (2002). "Place cells and place ... Participants are presented with a series of matrix patterns that have half their cells coloured and the other half blank. The ...

https://en.wikipedia.org/wiki/Spatial_memory

Cho ML, Kang JW, Moon YM, Nam HJ, Jhun JY, Heo SB, Jin HT, Min SY, Ju JH, Park KS, Cho YG, Yoon CH, Park SH, Sung YC, Kim HY ( ... The orphan nuclear receptor ROR-γ directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126:1121- ... Aggarwal S, Ghilardi N, Xie MH, de Sauvage FJ, Gurney AL (2003). "Interleukin-23 promotes a distinct CD4 T cell activation ... "Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells". Proc. Natl. Acad. Sci. U.S.A. 103 (21): ...

https://sh.wikipedia.org/wiki/Interleukin_17

PDF)You've got the power: the evolution of batteries and the future of fuel cells. Toshiba. Hentet 17. maj 2009.. ... Karki, Khim; Epstein, Eric; Cho, Jeong-Hyun; Jia, Zheng; Li, Teng; Picraux, S. Tom; Wang, Chunsheng; Cumings, John (2012-03-14 ... Battery Anodes , Batteries & Fuel Cells , Research , The Energy Materials Center at Cornell. Emc2.cornell.edu. Hentet 10. ...

https://da.wikipedia.org/wiki/Litium-6

Human embryonic stem cells also more readily differentiate into cortical stem cells in the presence of retinoids[8] ... Kafi, R; Kwak, HS; Schumacher, WE; Cho, S; Hanft, VN; Hamilton, TA; King, AL; Neal, JD; Varani, J; Fisher, GJ; Voorhees, JJ; ... Retinoids have many important functions throughout the body including roles in vision,[1] regulation of cell proliferation and ... Retinol enters the absorptive cells of the small intestine, preferentially in the all-trans-retinol form. ...

https://en.m.wikipedia.org/wiki/Retinoid

Meeyoung, Cho (4 August 2009). "South Korea unveils CO2 target plan". Reuters. Seoul. Retrieved 8 April 2010.. ... "UNEP and Daimler Call for Infrastructure for Electric and Fuel-cell Vehicles". Climate-L.org. 4 July 2008. Archived from the ...

https://en.wikipedia.org/wiki/United_Nations_Climate_Change_Conference_2009

CHO. {\displaystyle {{\ce {-CHO}}}}. ) ordezkatzen du eta kate bateko 2 lisinen eta beste kate paralelo bateko 2 lisinen ... a b c d e f g E., Sadava, David (1993) Cell biology : organelle structure and function Jones and Bartlett Publishers ISBN ... a b c d e f g (Ingelesez) «1416023887 - Cell Biology, Updated Edition: with Student Consult Online Access by Thomas D Pollard ... The Plant Cell Wall» Molecular Biology of the Cell. 4th edition . Noiz kontsultatua: 2018-12-08 . ...

https://eu.wikipedia.org/wiki/Zelula_kanpoko_matrize

cell cycle arrest. • learning or memory. • cellular copper ion homeostasis. • cellular response to copper ion. • cell cycle. • ... Hwang D, Lee IY, Yoo H, Gehlenborg N, Cho JH, Petritis B, Baxter D, Pitstick R, Young R, Spicer D, Price ND, Hohmann JG, ... cell surface. • endoplasmic reticulum. • membrane raft. • anchored component of membrane. • extracellular exosome. • cell ... PrP immune cells include hematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it ...

https://en.m.wikipedia.org/wiki/PRNP

Cell. Endocrinol. 370 (1-2): 138-48. doi:10.1016/j.mce.2013.02.016. PMC 3767392. PMID 23499866.. ... Mark Dennis; William Talbot Bowen; Lucy Cho (31 August 2016). Mechanisms of Clinical Signs - EPub3. Elsevier Health Sciences. ... the testes show Leydig cell hyperplasia, which is due to the increased levels of LH and intratesticular testosterone.[14] ... namely compromised follicular maturation via loss of estradiol signaling in ovarian granulosa cells.[14] ...

https://en.wikipedia.org/wiki/Estrogen_insensitivity_syndrome

The final structure of the abscess is an abscess wall, or capsule, that is formed by the adjacent healthy cells in an attempt ... Barbic, D; Chenkin, J; Cho, DD; Jelic, T; Scheuermeyer, FX (10 January 2017). "In patients presenting to the emergency ... The cytokines trigger an inflammatory response, which draws large numbers of white blood cells to the area and increases the ... However, such encapsulation tends to prevent immune cells from attacking bacteria in the pus, or from reaching the causative ...

https://en.wikipedia.org/wiki/Abscess

mast cell granule. • Schwann cell microvillus. • Schmidt-Lanterman incisure. • nucleoplasm. • cell projection cytoplasm. • ... Park J, Kim H, Park SY, Lim SW, Kim YS, Lee DH, Roh GS, Kim HJ, Kang SS, Cho GJ, Jeong BY, Kwon HM, Choi WS (May 2014). " ... The involvement in oxidative stress diseases, cell signal transduction and cell proliferation process endows AKR1B1 the ... cell signal transduction and cell proliferation process including cardiovascular disorders, sepsis, and cancer.[13] ...

https://en.wikipedia.org/wiki/AKR1B1

Islam MA, Yun CH, Choi YJ, Cho CS (2010). "Microencapsulation of live probiotic bacteria" (PDF). Journal of Microbiology and ... as well as increasing the proportion of T lymphocytes and natural killer cells.[96][97] LAB products might aid in the treatment ... or frozen yogurt products that contain 10 million cells per gram at the time of manufacture.[47] In 2002, the FDA and WHO ... but most companies that give a number report the viable cell count at the date of manufacture, a number that could be much ...

https://en.wikipedia.org/wiki/Probiotic?wprov=sfti1https://en.wikipedia.org/wiki/Probiotic?wprov=sfti1

Acharya, P. V., Goldman, D. S. Chemical composition of the cell wall of the H37Ra strain of Mycobacterium tuberculosis. Journal ... Ha, S., Jeon, B., Youn, J., Kim, S., Cho, S., Sung, Y. Protective effect of DNA vaccine during chemotherapy on reactivation and ... Herrmann, J., Lagrange, P. Dendritic cells and Mycobacterium tuberculosis: which is the Trojan horse?. Pathologie Biologie. ... Pai, M., Zwerling, A., Menzies, D. Systematic Review: T-Cell-Based Assays for the Diagnosis of Latent Tuberculosis Infection: ...

https://cs.wikipedia.org/wiki/Tuberkulóza

"In Wang, Yu-li; Discher, Dennis E. (eds.). Cell Mechanics. Methods in Cell Biology. 83. Elsevier Inc. pp. 473-493. ISBN 978-0- ... Min, Duyoung; Kim, Kipom; Hyeon, Changbong; Cho, Yong Hoon; Shin, Yeon-Kyun; Yoon, Tae-Young (2013-04-16). "Mechanical ... "The Journal of Cell Biology. 101 (1): 130-140. doi:10.1083/jcb.101.1.130. ISSN 0021-9525. PMC 2113644. PMID 4040136.. ... in whole cells. The phagocytosis method previously described is useful for capturing a magnetic bead inside a cell. Measuring ...

https://en.wikipedia.org/wiki/Magnetic_tweezers

From analyzing CHO cell lines, researchers found that the transcriptome of each subclone also had a significant number of ... individual changes and that such changes indicate that epigenetic regulation is a hidden but important player in cell line ... Phenotypic variations in the cells arise from changes in their DNA, including differences in methylation patterns. ... cells for early studies. To produce a CHO cell line that can be used in production, scientists use single-cell cloning. " ...

https://www.genengnews.com/topics/bioprocessing/cho-cell-lines-need-checking/

N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin ... The motilin pharmacophore in CHO cells expressing the human motilin receptor Biochem Biophys Res Commun. 2002 May 17;293(4): ... N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin ...

https://pubmed.ncbi.nlm.nih.gov/12054506/

The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ... Recombinant Protein Therapeutics from CHO Cells -- 20 Years and Counting. The CHO cell is at its height of technological ... This is a major driver in further understanding Chinese Hamster Ovary (CHO) cell biology, which is the cell line of choice for ... The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and ...

https://www.aiche.org/resources/publications/cep/2007/october/recombinant-protein-therapeutics-cho-cells-20-years-and-counting

PrecisION Ion Channel Cell Lines ,High Quality, Functionally-Validated, Ion Channel Cell Lines ,,Ion channels are well known ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate, ... hERG-CHO K1 Recombinant Cell Line from Upstate. 7. hERG-CHO K1 ... hHCN4-CHO K1 Recombinant Cell Line from Upstate. 9. hKv1.5-CHO K1 Recombinant Cell Line from Upstate. 10. hNav1.6-HEK293 ... hKv4.2/hKChIP2-CHO K1 Recombinant Cell Line from Upstate. 6. ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate. ...

http://www.bio-medicine.org/biology-products/hKvLQT1-hminK-CHO-K1-Recombinant-Cell-Line-from-Upstate-2314-1/

reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. ... The host cell line (CHOZN® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. Mild ... reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1 ... Overexpressing Mgat1 in wildtype CHO cells did not lead to increased sialylation ...

https://www.sigmaaldrich.com/technical-documents/articles/biology/mgat1-disrupted-chinese-hamster-ovary-cells.html

EXCELL Advanced CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ... CHO Feed 1 for superior platform performance in fed-batch cultures on all industrial CHO cell lineages (CHO-S, DuxB11, DG44, ... Determine the correct volume of cell culture to inoculate a new flask at a starting cell density of 2-3 x105 cells/ml in a ... EX-CELL® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed ...

https://www.sigmaaldrich.com/technical-documents/articles/biology/ex-cell-advanced-cho-fed-batch-medium.html

PrecisION Ion Channel Cell Lines ,High Quality, Functionally-Validated, Ion Channel Cell Lines ,,Ion channels are well known ... hKv4.2/hKChIP2-CHO K1 Recombinant Cell Line from Upstate, ... hERG-CHO K1 Recombinant Cell Line from Upstate. 6. hERG-CHO K1 ... hHCN4-CHO K1 Recombinant Cell Line from Upstate. 8. hKv1.5-CHO K1 Recombinant Cell Line from Upstate. 9. hNav1.6-HEK293 ... hKvLQT1/hminK-CHO K1 Recombinant Cell Line from Upstate. 11. Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem. ...

http://www.bio-medicine.org/biology-products/hKv4-2-hKChIP2-CHO-K1-Recombinant-Cell-Line-from-Upstate-2075-1/

A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros ... Chinese Hamster Ovary Cells (CHO-K1 Line). A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of ...

https://www.microscopyu.com/gallery-images/chinese-hamster-ovary-cells-cho-k1-line

Importantly, in cell-cell adhesion assays between CD2+ Jurkat T cells and CD48- or CD59-transfected CHO cells, there was no ... Gene context of CHO Cells. *Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3(-/-) chicken DT40 cells, indicating ... cells [15].. *Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the ... The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell ...

https://www.wikigenes.org/e/mesh/e/10946.html

... we have transfected each of these genes into Chinese hamster ovary cells (CHO-K1) and have established stable cell lines ... Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells.. Buckley NJ1, Bonner TI, Buckley ... National Institute of Mental Health, Laboratory of Cell Biology, Bethesda, Maryland 20892.. ...

https://www.ncbi.nlm.nih.gov/pubmed/2704370

Read the StainFree analysis of CHO cells using SoftMax Pro Software. ... CHO cells are an epithelial-like cell line commonly used in biological and medical research. ... CHO cells in these images were plated at 4000 cells per well in a 384-well microplate. Left: To create a new StainFree analysis ... To count CHO cells without staining, I use the predefined setting Cells A in SoftMax Pro Software. This works really well on ...

https://www.moleculardevices.com/applications/cell-counting/cell-counter-cho-cells

Dowd, J. E., Kwok, K. E. and Piret, J. M. (2001), Glucose-based optimization of CHO-cell perfusion cultures. Biotechnol. Bioeng ...

http://onlinelibrary.wiley.com/doi/10.1002/bit.10013/references?globalMessage=0

CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. Transport of ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... defective compared to vesicles from wild-type cells, whereas transport of other nucleotide sugars was normal. These cells do ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ...

https://www.thermofisher.com/au/en/home/technical-resources/cell-lines/c/cell-lines-detail-191.html

CHO glycosylation mutants. CHO-Lec1 cells completely lack complex and hybrid-type N-glycans on glycoproteins.. ... Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ...

https://www.thermofisher.com/jp/en/home/technical-resources/cell-lines/c/cell-lines-detail-202.html

Bjørn Voldborg, MSc, Director, CHO Cell Line Development, The Novo Nordisk Foundation Center for Biosustainability ... and since 2012 Bjørn has been heading the CHO Cell Line Engineering project at the NNF Center for Biosustainability at the ... Bjørn Voldborg of the Technical University of Denmark joins us to discuss the impact of big data on cell line engineering, his ... Production of Hard-to-Produce Proteins with CHO. ... to the engineering of improved protein production cell ...

https://www.healthtech.com/thechain/International-Collaboration-Yields-Therapeutic-Protein-With-Genome-Engineered-CHO-Cells/

Catalog Products » Other Products » Stable Cell Lines » Human Recombinant NK2 Tachykinin Receptor Stable Cell Line ... Human Recombinant NK2 Tachykinin Receptor Stable Cell Line Description. Tachykinins are peptides sharing a common C-terminal ...

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GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. ... GenScripts BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays.. ... Catalog Products » Other Products » Stable Cell Lines » Human Recombinant BB2 Bombesin Receptor Stable Cell Line ... Human Recombinant BB2 Bombesin Receptor Stable Cell Line Description. The bombesin receptor family is a member of the G protein ...

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... cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration ... Hiller GW, Ovalle AM, Gagnon MP, Curran ML, Wang WG (2017) Cell-controlled hybrid perfusion fed-batch CHO cell process provides ... CHO Site-specific integration CRISPR/Cas9 Cell line development C12orf35 Electronic supplementary material. The online version ... Li S, Gao X, Peng R, Zhang S, Fu W, Zou F (2016) FISH-based analysis of clonally derived CHO cell populations reveals high ...

https://link.springer.com/article/10.1007%2Fs00253-018-9021-6

CHO Stable Cell Line Development in Science/R&D with NGM Biopharmaceuticals, Inc.. Apply Today. ... Broad experience in cell biology, including mammalian cell culture, cell transfection, and cell-based functional assays ... Develop stable cell lines including clone screening, single cell cloning, and fed-batch production ... Evaluate the CHO CLD and production process with varies media. *Design studies and interpret results of development, ...

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... cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day- ... posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) ... Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i dont ... Have you done a viability assay to ensure that the floating cells are, in fact, dead?. CHO cells are damned hardy and will ...

http://www.protocol-online.org/forums/topic/15050-adhering-cho-cell-culture/

Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine ... Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine ... This also provide an explanation for the observed thermo-sensitive phenotype of CHO-MT58 cell line. ... The CHO-MT58 cell line expresses a mutant variant of CCT, and displays a thermo-sensitive phenotype. At non-permissive ...

https://doaj.org/article/cfe84253e72e440abf15cfd9a279ed69

... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells in DOAJ. DOAJ is an online ... cMyc increases cell number through uncoupling of cell division from cell size in CHO cells. BMC Biotechnology. 2009;9(1):76 DOI ... It is shown that the manipulation of cell cycle kinetics and indirectly cell metabolism gives higher cell densities in CHO ... gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the ...

https://doaj.org/article/ed5b13ff320c46559dc38a38b4e03ae3?frbrVersion=5

In AT1A receptor transfected CHO-K1 cells, angiotensin II (10−9 M) stimulated a rapid increase in cytosolic free calcium that ... Stable expression of a functional rat angiotensin II (AT1A) receptor in CHO-K1 cells: Rapid desensitization by angiotensin II. ... To address these problems, we expressed the recombinant AT1A receptor in CHO-K1 cells. The stably transfected receptor was ... high-level transfectant of the AT1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following ...

https://link.springer.com/article/10.1007%2FBF00926885

... but the only vendor I see refer to their cells as DG44 is at Invitrogen. All other cell line vendors just refer to them as CHO ... posted in Cell Biology: I am looking to purchase DG44 cells because these are the cells that I see appear in the literature ... I know that DG44 cells are dhfr- mutants, ... The Differences Between CHO DG44 and CHO dhfr- - ... All other cell line vendors just refer to them as CHO dhfr-. Is there an actual difference do you think between cells ...

http://www.protocol-online.org/forums/topic/25812-the-differences-between-cho-dg44-and-cho-dhfr/

Keywords: Animals ; Bioreactors ; CHO Cells/*physiology ; Cell Culture Techniques/methods ; Cell Proliferation ; Cell Survival ... CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear ... Serum-free large-scale transient transfection of CHO cells. Derouazi, M.; Girard, P.; Van Tilborgh, F.; Iglesias, K.; Muller, N ... The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected ...

https://infoscience.epfl.ch/record/105019

... simple luminescent calcium flux assays using irradiated AequoScreen cells tranfected with human GPR99 receptor - no cell ... Human Recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells ... The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we ... Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you ...

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Outward Current in wild type CHO cells I am getting about a 0.5nA outward current in untransfected i.e. wild type CHO cells at ... CCL-61 CHO-K1 cells if you want to try those instead of the ones you are using. They just dont express hERG as well as the ... Some literature suggests there are no functional potassium channels in wild type CHO and HEK cells although Frasermoss recently ... Home/ Forums/ Anatomy and Physiology: Electrophysiology/ Outward Current in wild type CHO cells. ...

https://scientistsolutions.com/forum/anatomy-and-physiology-electrophysiology/outward-current-wild-type-cho-cells

div class=section, ,h2,Reduce the Time Needed to Isolate Producing, Monoclonal Cell Lines,/h2, ,h3,Background,/h3, ,p,The ... ClonaCell™-CHO methylcellulose-based semi-solid media are specifically designed for cloning of CHO cell lines. ClonaCell™-CHO ... Perform cell transfection.. *Incubate the cells in liquid recovery medium (such as ClonaCell™-CHO CD Liquid, Catalog #03817) ... Using the standard protocol for mammalian cell cloning in ClonaCell™-CHO media, freshly transfected cells are suspended in ...

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CHO Cell) Contact info: e-mail:[email protected] skype:live:sunnylidi whatsapp:+8613735524230 http://www. ...

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... Pan, Xiao; Streefland, Mathieu; Dalm, Ciska; ... Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell ...

https://brage.bibsys.no/xmlui/handle/11250/2433531

Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production. (springer.com)
Bachu R, Bergareche I, Chasin LA (2015) CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining. (springer.com)
Over the past decades, the increase in maximal cell numbers for the production of mammalian derived biologics has been in a large part due to the development of optimal feeding strategies. (doaj.org)
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). (epfl.ch)
Conventional methods to select and clone mammalian cell lines involve multiple dilution steps in liquid medium. (stemcell.com)
Using the standard protocol for mammalian cell cloning in ClonaCell™-CHO media, freshly transfected cells are suspended in selective semi-solid medium and incubated in 10 cm plates. (stemcell.com)
Transient transfection of mammalian cells in suspension culture has recently emerged as a very useful method for production of research-scale quantities of recombinant proteins. (nih.gov)
Mammalian expression systems, particularly Chinese hamster ovary (CHO) cells, are used industrially for the production of biopharmaceuticals. (bl.uk)
Despite improvements over the last few decades with regard to the maximum cell concentrations obtainable and the amount of recombinant protein produced from such expression systems, there is still the potential to engineer and manipulate mammalian cells to generate more robust host cells that grow faster, for longer and produce higher product yields with less heterogeneity. (bl.uk)
A number of recent studies have reported that mRNA translation is a key limitation in terms of defining the product yield from in vitro cultured mammalian cells. (bl.uk)
This is one of the first such studies to investigate the effect of manipulating multiple miRNA on recombinant protein synthesis from mammalian cells. (bl.uk)
Chemically defined media free of animal-derived components are required for bioprocess operations based on mammalian cells. (bioprocessintl.com)
The purpose of this project was to investigate techniques that may be successfully applied to improve the characteristics of a bioprocess relevant mammalian cell line such as CHO (Chinese Hamster Ovary). (dcu.ie)
Eurofins Lancaster Laboratories offers capabilities to prepare and characterize a wide variety of mammalian, insect and avian cell banks, including master, working and research banks. (eurofinsus.com)
Production of recombinant protein therapeutics in cultivated mammalian cells. (springer.com)
Among the variables that are appropriate for direct feedback control of the perfusion rate in mammalian cell cultures, high priority should be given to the glucose concentration. (bioprocessintl.com)
In summary, the proposed glucose monitoring and control technique proved to be a reliable tool which can be applied with confidence at an industrial scale for either microbial or mammalian cell cultures. (bioprocessintl.com)
Temperature-sensitive miR-483 is a conserved regulator of recombinant protein and viral vector production in mammalian cells. (semanticscholar.org)
Because this cell line has been immortalized, its genome has accumulated many mutations, thus impairing certain biochemical pathways in CHO mammalian cells. (selexis.com)
By using specifically designed bioinformatics to analyze data from trancriptome and genome sequencing , Selexis has been able to generate tools to engineer CHO mammalian cells and repair defined biochemical pathways that enable the expression of difficult-to-express proteins. (selexis.com)
These libraries significantly de risk expression and production issues of difficult-to-express recombinant proteins in CHO mammalian cells, thus helping reduce attrition rates in biologic drug discovery and development. (selexis.com)
Selexis' innovation and expertise have enabled the development and ongoing optimization of the most robust and flexible mammalian cell line-based protein expression platform in the industry. (selexis.com)
The SURE technology Platform improves the way that mammalian cells are used in the discovery, development, and manufacturing of any recombinant protein drug. (selexis.com)
Gudi, R. and E.H. Schadly (1997) In vitro mammalian cytogenetic test using Chinese hamster ovary (CHO) cells with Granola 97 (SCJ NB # 14735R108). (epa.gov)
In a mammalian cell chromosomal aberration cytogenetics assay (MRID 44438708), Chinese hamster ovary CHO-K1 cell cultures were exposed to Granola 97 (98.3% a.i., batch No. 703001) in DMSO in two independent assays. (epa.gov)

They are highly amenable to transfection and have become the most popular cell line for manufacturing recombinant proteins, including therapeutics. (moleculardevices.com)
Percent confluence of cells just prior to transfection was determined using StainFree technology. (moleculardevices.com)
To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. (nexcelom.com)
I am looking to purchase DG44 cells because these are the cells that I see appear in the literature most often for transient transfection and recombinant protein production for commercial uses. (protocol-online.org)
CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear 25 kDa PEI as a transfection vehicle. (epfl.ch)
Parameters that were optimized included the DNA amount, the DNA-to-PEI ratio, the timing and solution conditions for complex formation, the transfection medium, and the cell density at the time of transfection. (epfl.ch)
On the day of transfection, place ClonaCell™-CHO methylcellulose-based semi-solid medium (Catalog #03815, 03816) at 2 - 8°C to thaw overnight. (stemcell.com)
Perform cell transfection. (stemcell.com)
On the day after transfection, warm thawed ClonaCell™-CHO semi-solid medium to 37°C. (stemcell.com)
Plating at various cell densities is recommended as transfection efficiency may vary from experiment to experiment. (stemcell.com)
We report here that the plasma exposure in mice of an IL-23R extracellular domain Fc fusion protein (IL23R-Fc) differed dramatically depending on whether the protein was prepared by transient transfection of HEK or CHO cells. (nih.gov)
The GS-KO selection system allows for identification of multiple-gram/L clones in a timeframe of two to three months from initial transfection, with such performance demonstrated consistently across the different providers of GS-KO CHO cells. (bioprocessintl.com)
The results demonstrate the importance of the osmotic shock on the efficient delivery of plasmid DNA to the nucleus of CHO cells following calcium phosphate-mediated transfection. (epfl.ch)
For HEK293 cells producing Gag-GFP virus-like particles (VLPs), we used polyethylenimine transient transfection. (bioprocessintl.com)
The best options for cell growth are not necessarily the best for transient transfection. (bioprocessintl.com)
The optimal media for cell growth are not best for transient transfection, and vice versa. (bioprocessintl.com)
Three of the four we tested had a more positive effect on cell growth and transient transfection, and we retained those. (bioprocessintl.com)
Production of mAb by recombinant expression using transient transfections of cells can lead to a batch to batch variability and the mAb yield is influenced by the passage number and quality of the cultured cells, transfection efficiency and the toxicity of transfection reagents. (pubmedcentralcanada.ca)
Additionally, many (commonly available) cell lines used for transient transfection are serum-dependent, resulting in contamination of produced mAbs by bovine IgGs. (pubmedcentralcanada.ca)
In recombinant cell lines, exogenous dhfr (together with the gene of interest, (GOI)) is provided by a vector used for transfection, making additional supplementation by HT unnecessary. (pubmedcentralcanada.ca)
High Throughput Transfection of Stem Cells, Primary Cells and Difficult-to-Transfect Cell Lines: Jurkat, CHO, Human Skeletal Muscle Cells & Primary Neuronal Cell Transfection using a Scalable, Electroporation-Based Technology. (selectscience.net)
Data in this poster is presented for the transient transfection of a variety of difficult-to-transfect cells and their use in downstream assays. (selectscience.net)
Specifically, the transfection of Jurkat,CHO, human skeletal muscle cells and primary neuronal cells using MaxCyte's scalable electroporation technology and their performance in a variety of cell-based assays is described. (selectscience.net)
This demonstrates how large-scale electroporation can be used to eliminate the reliance on stable cell lines and costly transfection reagents, by producing large numbers of quality transfected cells for use in high throughput cellular screening and profiling. (selectscience.net)
After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO(BRI/rcTA) pools producing high levels of recombinant proteins. (physiciansweekly.com)

This medium is designed to be used in conjunction with Advanced™ CHO Feed 1 for superior platform performance in fed-batch cultures on all industrial CHO cell lineages (CHO-S, DuxB11, DG44, CHO-M, and CHOZN GS). (sigmaaldrich.com)
I know that DG44 cells are dhfr- mutants, but the only vendor I see refer to their cells as DG44 is at Invitrogen. (protocol-online.org)
Is there an actual difference do you think between cells designated DG44 and those designated CHO dhfr- ? (protocol-online.org)
CHO DG44 (mAb producer) cell growth and productivity in 50 L bioreactor using HyClone ActiPro medium. (labonline.com.au)
The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. (epfl.ch)
Here, we report the development of a serum-free CHO DG44 cell line, stably producing a CR9114-like antibody with a potential to become a useful influenza virus research tool. (pubmedcentralcanada.ca)
Here, we decided to take advantage of a biotechnology-relevant production cell line, Chinese hamster ovary (CHO) DG44, to establish a serum-free, stable, CR9114-like (CR9114L, a generic version of CR9114) antibody-producing cell line for a steady supply of this mAb with low batch to batch variation. (pubmedcentralcanada.ca)
The CHO DG44 cell line, in which both alleles are dihydrofolate reductase (DHFR) negative, was created in 1980ies by ionizing radiation [ 7 ]. (pubmedcentralcanada.ca)
Therefore, CHO DG44 host cells medium requires supplementation by hypoxanthine and thymidine (HT). (pubmedcentralcanada.ca)
Next, a label-free quantitative proteomics analysis of CHO-S and CHO DG44 cell lines and liver and ovary tissue identified 11801 proteins, including 9359 proteins specifically in the cell lines, representing a 56% increase over previous work. (jhu.edu)

I have read that non essential amino acids can favor suspension CHO cell cultures but I'm not so sure of it. (protocol-online.org)
It is shown that the manipulation of cell cycle kinetics and indirectly cell metabolism gives higher cell densities in CHO batch cultures. (doaj.org)
The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected with 2.5 microg/ml DNA in RPMI 1640 medium containing 25 mM HEPES at pH 7.1. (epfl.ch)
Results from shake flask cultures show high viable cell densities and protein production. (labonline.com.au)
The objective of this study was to evaluate the effect of UV irradiation of cell culture media in terms of chemical composition and the ability to grow cell cultures in the treated media. (uwaterloo.ca)
During many such biphasic cultures, the initial phase of rapid cell growth at 37 degrees C is followed by a growth arrest phase induced through reduction of the culture temperature. (semanticscholar.org)
Temperature reduction in cultures of hGM-CSF-expressing CHO cells: effect on productivity and product quality. (semanticscholar.org)
Chinese Hamsters are considered by FWS to be regulated wildlife so shipments of actual CHO cells, cultures or cell lines require importers to obtain a FWS license and declare their import shipments for clearance. (marken.com)
Cell cultures from silkworm and spider silk producing cells were also pursued. (usu.edu)

ClonaCell™-CHO ACF is animal component-free and contains recombinant proteins. (stemcell.com)
In contrast to their differing plasma exposures, the HEK- and CHO-expressed proteins had equivalent in vitro biological activity. (nih.gov)
Characterization of the CHO- and HEK-expressed IL23R-Fc proteins indicated that the differences in in vivo plasma exposure between them are due to differential glycosylation. (nih.gov)
Most widely used cell lines for biopharmaceutical production of therapeutic proteins originate from CHO cells. (labonline.com.au)
Chinese hamster ovary (CHO) cells have been used in biomanufacturing for decades because of their robust capacity to express a range of proteins, such as therapeutic enzymes and monoclonal antibodies (MAbs) at titers measured in multiple grams per liter of culture. (bioprocessintl.com)
We began with existing commercial media and supplemented them with specific proteins to improve cell growth. (bioprocessintl.com)
Chinese Hamster Ovary (CHO) cells are the biopharmaceutical industry's "mini biofactories" for the production of complex, post-translationally modified therapeutic proteins. (dcu.ie)
CAMBRIDGE, Mass., Nov. 8, 2010 (GLOBE NEWSWIRE) -- Momenta Pharmaceuticals, Inc. (Nasdaq:MNTA), a biotechnology company specializing in the characterization and engineering of complex mixture drugs, today announced that the scientific journal Nature Biotechnology has published a correspondence entitled "Chinese Hamster Ovary Cells Can Produce Galactose-alpha-1,3-Galactose Antigens on Proteins. (nbcnews.com)
CHO cells are widely used for the manufacture of biotherapeutics, in part because of their ability to produce proteins with desirable properties, including "human like" glycosylation profiles. (nbcnews.com)
CHOMACS medium supports cell growth and production of recombinant proteins and antibodies in suspension culture. (miltenyibiotec.com)
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). (physiciansweekly.com)
We have developed a cell line (CHO(BRI/rcTA)) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. (physiciansweekly.com)
These results suggest that the cumate-inducible CHO(BRI/rcTA) stable pool platform is a powerful and robust system for the rapid production of gram amounts of recombinant proteins. (physiciansweekly.com)
Chinese hamster ovary (CHO) cells are predominant in the production of therapeutic proteins to treat various diseases. (springer.com)
BACKGROUND To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary phase where cellular energies are directed towards production of rP. (semanticscholar.org)
We continuously innovate to provide better solutions for our customers, and we are excited about the exceptional yield gains seen in the manufacture of recombinant proteins from CHO cells grown in the HyCell CHO medium," said Tariq Haq, senior global product manager for media and buffers, Thermo Scientific Cell Culture and Bioprocessing products. (technologynetworks.com)
However, shipments of specimens or other CHO derived products that have been processed to the extent that animal material no longer remains in the specimen (e.g., antibodies or recombinant proteins extracted from CHO cells and purified to remove all cell material) do not require FWS import license or agency clearance. (marken.com)
CHO (Chinese hamster ovary) cells are the most dependable host cells for large-scale production of therapeutic proteins and have been used in the pharmaceutical industry for over 60 years. (selexis.com)
Because optimal expression of certain proteins can not be achieved by elevated transcription alone, this platform offers an unprecedented solution for generating manufacturing cell lines that are specifically tailored to the particular expression and secretory needs of many of difficult-to-express recombinant proteins. (selexis.com)
Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs)-Alpha Diagnostic International Inc. (4adi.com)
Cell lines from spider and silkworm silk producing glands appeared to have the capacity to secrete full-length native proteins ranging in size from 200 to 500 kDa, and possibly larger. (usu.edu)
Antibodies-Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure-Alpha Diagnostic International Inc. (4adi.com)
Kit-Chinese Hamster Ovary Cell (CHO) host cell Proteins (HCPs) ELISA kit, 96 tests-Alpha Diagnostic International Inc. (4adi.com)

From analyzing CHO cell lines, Weinguny and his colleagues found that "the transcriptome of each subclone also had a significant number of individual changes," and the scientists pointed out that such changes "indicate that epigenetic regulation is a hidden, but important player in cell line development with a major role in the establishment of high performing clones with improved characteristics for bioprocessing. (genengnews.com)

To produce a CHO cell line that can be used in production, scientists use single-cell cloning. (genengnews.com)
N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin receptor and apoaequorin. (nih.gov)
A functional hamster MGAT1 was overexpressed in an Mgat1-disrupted IgG producing cell line (Mgat1 KO37). (sigmaaldrich.com)
The host cell line (CHOZN ® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. (sigmaaldrich.com)
Mgat1KO37OE4 expresses Mgat4b significantly higher, while OE12's Mgat4b is significantly lower, than the non-ZFN transfected cell line. (sigmaaldrich.com)
Mgat1KO37 has significantly decreased Mgat4b than non-ZFN transfected cell line where it was derived from. (sigmaaldrich.com)
The host cell line that does not express IgG has significantly higher Mgat4b than the IgG-expressing non-ZFN transfected line. (sigmaaldrich.com)
Chinese Hamster Ovary (CHO) cells are an epithelial-like cell line commonly used in biological and medical research. (moleculardevices.com)
Bjørn Voldborg of the Technical University of Denmark joins us to discuss the impact of big data on cell line engineering, his team's success in producing Alpha-1 antitrypsin, the appeal of applied science, and more. (healthtech.com)
From this he went to the NNF Center for Protein Research at the University of Copenhagen as Head of the Protein Production Unit, and since 2012 Bjørn has been heading the CHO Cell Line Engineering project at the NNF Center for Biosustainability at the Technical University of Denmark, a project dedicated to the engineering of improved protein production cell factories. (healthtech.com)
GenScript's BB2-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. (genscript.com)
However, by conventional random integration strategy, development of a high-expressing and stable recombinant CHO cell line has always been a difficult task due to the heterogenic insertion and its caused requirement of multiple rounds of selection. (springer.com)
In comparison with a random integration control, all the targeted integration cell lines showed higher productivity, among which C12orf35 locus was the most advantageous in both productivity and cell line stability. (springer.com)
Damavandi N, Raigani M, Joudaki A, Davami F, Zeinali S (2017) Rapid characterization of the CHO platform cell line and identification of pseudo attP sites for PhiC31 integrase. (springer.com)
CHO Stable Cell Line Development job with NGM Biopharmaceuticals, Inc. (biospace.com)
They grow quite well according to their passage number, even the cell line with 86 passages grows although slower than the one on passage 5. (protocol-online.org)
By the way, it also happens to the parental CHO-K1 line, so that's even trickier. (protocol-online.org)
Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine Cytidylyltransferase. (doaj.org)
The CHO-MT58 cell line expresses a mutant variant of CCT, and displays a thermo-sensitive phenotype. (doaj.org)
This also provide an explanation for the observed thermo-sensitive phenotype of CHO-MT58 cell line. (doaj.org)
Engineering of the cell line is one of probable approaches for increasing cell numbers in bioreactor. (doaj.org)
cell line was found to be within 0.7% of the control. (doaj.org)
over-expressing cell line did not increase with the increase in cell number. (doaj.org)
Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, date and your initials. (openwetware.org)
It seems that they are probably the same cell line - Urlaub and Chasin seem to be the names mentioned with them, and they were at least collaborators on the paper describing a DHFR- CHO cell line. (protocol-online.org)
Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields of ~1.8 g/l. (frontiersin.org)
POTELLIGENT([R]) Technology involves the reduction of the amount of fucose in the carbohydrate structure of an antibody using a proprietary fucosyl transferase-knockout CHO cell line as a production cell. (thefreedictionary.com)
The first EX-CELL Advanced product is the batch media system developed for a range of widely used industrial CHO cell lines, including SAFC's CHOZN cell line. (thefreedictionary.com)
With so many variants of the CHO cell line and so many generic media and associated supplements, it seems difficult to just pick one media and associate it with any CHO-derived cell line. (thefreedictionary.com)
We offer an extensive breadth of process development capabilities from cell line and strain development, using our proprietary pAVEway[TM] and CHO cell line systems, to process development, analytical development, clinical and commercial manufacturing. (thefreedictionary.com)
Cell Biologist with experience in CHO cell line development needed for growing biotechnology company. (thefreedictionary.com)
Horizon's GS-KO CHO SOURCE cell line is available with an unlimited use license without those prohibitive restrictions and reach-through costs. (bioprocessintl.com)
Additionally, the company allows for and actively encourages cell-line modifications to improve biomanufacturing performance. (bioprocessintl.com)
CHO-HuCLCN1 cell line is clonally-derived from a CHO cell line, which has been transfected with a human chloride channel, voltage-sensitive 1 (CLCN1) to allow stably express of the human CLCN1. (creativebiomart.net)
It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. (creativebiomart.net)
Protein expression: CLCN1 expression in this cell line has been validated by WB.3. (creativebiomart.net)
CHO-HuTRPM4 cell line is clonally-derived from a CHO cell line, which has been transfected with a human transient receptor potential cation channel, subfamily M, member 4 (TRPM4) to allow stably express of the human TRPM4. (creativebiomart.net)
Goh JB, Ng SK (2017) Impact of host cell line choice on glycan profile. (springer.com)
Grainger RK, James DC (2013) CHO cell line specific prediction and control of recombinant monoclonal antibody N-glycosylation. (springer.com)
In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. (scielo.br)
Using this technique we successfully isolated a number of mutant clones with average maximum viable cell densities 53% greater than a control cell line when placed through the same stress selective procedure. (dcu.ie)
Finally we designed single guide RNAs to target Cas9 to the miR-7a-5p genomic locus to disrupt miR-7 in order to enhance growth of a CHO-K1 cell line producing an IgG-1. (dcu.ie)
Horizon Discovery Group plc (LSE: HZD) ("Horizon"), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase ("GS") knockout Chinese Hamster Ovary (CHO) K1 cell line. (horizondiscovery.com)
Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Company's biomanufacturing processes. (horizondiscovery.com)
After extensive evaluation, Horizon's GS knockout CHO K1 cell line demonstrated consistently impressive performance. (horizondiscovery.com)
We are extremely encouraged by the adoption of our GS knockout CHO K1 cell line by Glenmark. (horizondiscovery.com)
These licenses have been taken as a result of an unmatched combination of high performance, transparent cell line history, supporting documentation, and attractive licensing terms. (horizondiscovery.com)
The system includes the GS knockout CHO K1 cell line, a comprehensive package of supporting documentation, and an expression vector supplied under license from DNATwoPointO, Inc.. This biomanufacturing platform allows these companies to move from the DNA sequence of their potential biotherapeutic to clinical manufacturing as simply and rapidly as possible. (horizondiscovery.com)
The interaction of protective antigen (PA), a component of the anthrax toxin, with receptors on the Chinese hamster ovary cell line CHO-K1 was characterized. (asm.org)
CHO-HuGABRA5/GABRB2/GABRG2 cell line is clonally-derived from a CHO cell line, which has been transfected with a human gamma-aminobutyric acid (GABA) A receptor, alpha 5 (GABRA5), a human gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2) and a human gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2) to allow stably express of the human GABRA5, GABRB2 and GABRG2. (creativebiomart.net)
Protein expression: GABRA5/GABR2/GABRG2 expression in this cell line has been validated by WB.3. (creativebiomart.net)
The GeneBLAzer® MC2R-CRE-bla-CHO-K1 cells contain the human melanocortin-2 receptor (MC2R), (Accession # NM_000529.1) and the Melanocortin-2 receptor accessory protein (MRAP), (Accession #NM_178817.3) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. (caigou.com.cn)
Characterization and investigation of CHO cell metabolism in a quick and simple way could boost process and cell line development. (springer.com)
A comparison between Alternating Tangential Flow (ATF) and Tangential Flow Filtration (TFF) was performed using a recombinant CHO cell line producing a monoclonal antibody (mAb) as a model system. (diva-portal.org)
Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. (diva-portal.org)
Selecting the right medium to optimally match your cell line and process can be challenging. (merckmillipore.com)
Our Cellvento® CHO media portfolio provides you with process and cell line specific cell culture media and feeds - so that you can find the most suitable product for your application. (merckmillipore.com)
As a result, there are several limitations in the range of products that can be expressed and secreted by this cell line. (selexis.com)
Selexis SA, a JSR Life Sciences Company, is the global leader in cell line development with best-in-class modular technology and highly specialized solutions that enable the life sciences industry to rapidly discover, develop and commercialize innovative medicines and vaccines. (selexis.com)
Our global partners are utilizing Selexis cell line development technologies to advance more than 115 drug products in clinical development and the manufacture of five commercial products. (selexis.com)
We believe that our cell line development technologies offers solutions that no one else is able to provide. (selexis.com)
In addition, expressing PKCbeta in a parietal endoderm cell line caused these cells to retrodifferentiate into stem cells. (aacrjournals.org)
The CHO 1-15 cell line protocols were optimized by establishing conditions for reproducibility in shaker flasks and bioreactors (2 to 250 L capacity). (usu.edu)
CHO 1-15 is the cell line of choice for protein production in the bio-manufacturing industry. (usu.edu)
The effects of the antioxidant nature of these compounds were tested on the CHO 1-15 cell line. (usu.edu)
Methods to produce a primary cell line from spider silk gland cells were developed. (usu.edu)
Centinel™ technology provides manufacturers with an additional path for mitigating the risk of MVM contamination, while maintaining an equivalent level of protein quality and cell line productivity. (merckmillipore.com)
The company's BioReliance® testing services can validate MVM resistance and demonstrate the virus is not propagated in the cell line. (merckmillipore.com)
First, wild-type CHO was compared with tunicamycin-treated CHO and Lec9.4a cells, a mutant CHO cell line which shows 50% of wild-type glycosylation levels. (jhu.edu)
Styrylpyrone Derivative (SPD) induces apoptosis in a caspase-7-dependent manner in the human breast cancer cell line MCF-7. (nih.gov)
Here, we used the caspase-3-deficient MCF-7 cell line. (nih.gov)

This study set out to investigate the role of specific miRNAs on determining CHO cell growth and recombinant protein productivity. (bl.uk)

These cells should be robust such that a relatively generic platform process, such as one for monoclonal antibodies, could be applied with very little or no process development to rapidly create bulk drug substance for Phase I clinical trials," Beske says. (genengnews.com)

Some of these components can induce in treated cells a type of cell death known as apoptosis (5, 23, 26, 40). (scielo.br)
Apoptosis is a programmed cell death with known morphological changes in the nucleus (24), actin filaments (16, 27) and endoplasmic reticulum (17, 32). (scielo.br)
TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. (biovendor.com)
Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues. (springer.com)
2 When administered in animal models, GSIs have a range of biological activities, including an ability to reduce the accumulation of amyloid peptides associated with Alzheimer's disease, and can induce apoptosis in a variety of tumors, included breast cancer cells. (pubmedcentralcanada.ca)
Apoptosis inhibited by DEVD-CHO. (nih.gov)
RESULTS: We found that the intrinsic apoptotic pathway was invoked, with the accumulation of cytosolic cytochrome c and processing of the initiator caspase-9.To confirm that apoptosis was induced following caspase-7 activation, the caspase inhibitor Ac-DEVD-CHO was used.CONCLUSIONS: Taken together, these results suggest SPD as a potent antiproliferative agent on MCF-7 cells by inducing apoptosis in a caspase-7-dependent manner. (nih.gov)
To confirm that apoptosis was induced following caspase-7 activation, the caspase inhibitor Ac-DEVD-CHO was used. (nih.gov)
Pre-incubation of cells with this inhibitor reversed apoptosis levels and caspase-7 activity in SPD-treated cells to untreated levels. (nih.gov)
CONCLUSIONS: Taken together, these results suggest SPD as a potent antiproliferative agent on MCF-7 cells by inducing apoptosis in a caspase-7-dependent manner. (nih.gov)
When MCF-7 cells were incubated with the caspase-7 inhibitor, DEVD-CHO at (A) 50 μM and (B) 100 μM, prior to SPD treatment, apoptosis levels decreased to control untreated levels as detected by nuclear staining, suggesting the important role played by this executioner caspase in SPD-induced apoptosis. (nih.gov)
Also, preincubation of MCF-7 cells with this inhibitor at 50 μM to 100 μM inhibited apoptosis and brought apoptotic levels down to the level similar to controls (Figure 5), thus purporting an apoptotic pathway dependent on caspase-7. (nih.gov)

Our results demonstrated the feasibility of C12orf35 as the target site for exogenous gene integration, and strongly suggested that C12orf35 targeted integration mediated by CRISPR/Cas9 is a reliable strategy for the rapid development of recombinant CHO cell lines. (springer.com)
Ding K, Han L, Zong H, Chen J, Zhang B, Zhu J (2017) Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein. (springer.com)
gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the control. (doaj.org)
As ActiPro medium and Cell Boost 7a and 7b supplements do not contain hypoxanthine or thymidine (HT), their formulation supports the dihydrofolate reductase (DHFR) gene amplification and selection system. (labonline.com.au)
We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. (portlandpress.com)
Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. (portlandpress.com)
Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. (portlandpress.com)
A considerable amount of optimisation work was performed to establish the CRISPR system for use in the lab, initially using eGFP as model target gene in CHO cells. (dcu.ie)
CellSensor® CRE-bla CHO-K1 cells (Cat # K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP Response Element (CRE). (caigou.com.cn)
Point mutation in the Virbrio cholerae O139 cef (CHO cell elongating factor) gene alters the. (deepdyve.com)
Shalu, O. 2012-02-20 00:00:00 Sequencing of the cef (CHO cell elongating factor) gene of Vibrio cholerae serogroup O139 revealed one nucleotide substitution (T to C at nucleotide 2015) as compared to cef of classical V. cholerae O1 and two substitutions (GT to AC at nucleotides 2014-2015) as compared to cef of V. cholerae O1 El Tor. (deepdyve.com)
Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch. (physiciansweekly.com)
Based on these results, we conclude that PKCbeta and PKCalpha are key targets for RA-regulated gene expression, that PKCalpha plays an important, active role in inducing and maintaining the parietal endoderm phenotype, and that PKCbeta activity is incompatible with maintaining the differentiated state of these cells. (aacrjournals.org)
Darmstadt, Germany, September 8, 2016 - Merck , a leading science and technology company, today launched a first-of-its-kind gene editing technology to modify CHO cell lines to be resistant to minute virus of mice (MVM), a common contamination threat that remains despite the shift to chemically defined, animal component-free manufacturing processes. (merckmillipore.com)
Overall, both gene ontology and KEGG pathway analysis revealed enrichment of cell cycle activity in cells. (jhu.edu)

Other experts agree on the value of reliable CHO cell lines. (genengnews.com)
Given the wide use of CHO cells, reliable and vigorous lines are exactly what researchers and drug developers need. (genengnews.com)
Chinese Hamster Ovary (CHO) cell lines are preferred host cells for therapeutic protein production. (nih.gov)
Upstate has built a portfolio of ion channel cell lines to meet these new and evolving needs in the drug discovery industry. (bio-medicine.org)
Our ion channel cell lines are available off of the shelf. (bio-medicine.org)
These cell lines are fully validated using electrophysiology, stable (>25 passage) and licensed for drug discovery research. (bio-medicine.org)
The objective is to provide the pharmaceutical industry with a range of ion channel cell lines to support drug discovery, lead optimisation and safety pharmacology functions. (bio-medicine.org)
Zinc-finger nuclease (ZFN) technology was used to create our Mgat1-disrupted CHO lines with high-mannose dominant simple N-glycoforms. (sigmaaldrich.com)
The results support the approach of Mgat1 and Mgat4 co-overexpression to create host cell lines for biopharmaceutical production with increased sialylation. (sigmaaldrich.com)
In order to investigate the pharmacological properties of the individual muscarinic receptors, we have transfected each of these genes into Chinese hamster ovary cells (CHO-K1) and have established stable cell lines expressing each receptor. (nih.gov)
Site-specific integration of transgenes into CHO hot spots is an ideal strategy to overcome these challenges since it can generate isogenic cell lines with consistent productivity and stability. (springer.com)
In this study, we investigated three sites with potential high transcriptional activities: C12orf35, HPRT, and GRIK1, to determine the possible transcriptional hot spots in CHO cells, and further construct a reliable site-specific integration strategy to develop recombinant cell lines efficiently. (springer.com)
Stable cell lines were generated successfully after a single round of selection. (springer.com)
Deep sequencing demonstrated that there was low level of off-target mutations caused by CRISPR/Cas9, but none of them contributed to the development process of transgene cell lines. (springer.com)
Our platform has been validated against experimental data from two IgG-producing CHO cell lines representing all aforementioned scales. (aiche.org)
I have to start a lot of infection experiments on these cell lines and i need to be sure they are worth the effort! (protocol-online.org)
ClonaCell™-CHO methylcellulose-based semi-solid media are specifically designed for cloning of CHO cell lines. (stemcell.com)
The most commonly used cell lines for this purpose are suspension-adapted HEK and CHO cells. (nih.gov)
Read the detailed application note to learn more about the methods and results with other CHO cell lines here . (labonline.com.au)
Within the available suite of CHO cell lines, the glutamine synthetase knockout (GS-KO) selection system provides industry-leading speed to the identification of high-producing clones for use in biomanufacturing. (bioprocessintl.com)
Horizon Discovery believes that industry should have easier access to the best manufacturing cell lines. (bioprocessintl.com)
1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. (creativebiomart.net)
In this study we determined the profile of four target miRNA (miR-15b, miR- 16, miR-21 and miR-34c) throughout batch culture in model monoclonal antibody producing CHO cell lines and the effect of transiently over-expressing the corresponding pre-miRNA individually or together. (bl.uk)
There was no relationship between pri-microRNA amounts and the mature microRNA amounts observed in the model cell lines investigated. (bl.uk)
Supplementation of commercial media with specific compounds was studied using a design of experiments (DoE) approach to screen the most efficient compounds for two cell lines and then determine their optimal conditions. (bioprocessintl.com)
We used these approaches systematically with two different cell lines: HEK293 and Chinese hamster ovary (CHO) cells. (bioprocessintl.com)
Cultivation of different CHO and other animal cell lines. (miltenyibiotec.com)
Incorporating this technology into our biomanufacturing processes enhances our ability to efficiently generate high quality cell lines. (horizondiscovery.com)
All of these services are provided with strict adherence to cGMP requirements and are designed to fully support the testing of your biopharmaceutical product, including the testing of raw materials, cell lines, unprocessed bulk , purified bulk and final product. (eurofinsus.com)
As GSI-I (Z-LLNle-CHO) is also a derivative of a widely used proteosome inhibitor MG-132, we hypothesized that this compound might be active in precursor-B acute lymphoblastic leukemia (ALL) cell lines and patient samples. (pubmedcentralcanada.ca)
Recent studies in multiple myeloma and in lymphoma-derived B-cell lines suggest that GSI sensitivity may be a general property of mature B-cell neoplasms. (pubmedcentralcanada.ca)
Cellvento® CHO media have been developed for use with CHO suspension cell types such as CHO-S, CHO-DHFR- and CHO-K1 cells, but may also be suitable for other cell lines. (merckmillipore.com)
WGS provides Selexis partners with an in-depth analysis of their recombinant cell lines and allows for these cell lines to be barcoded to facilitate tracking of clonal drift or the identification of adventitious agents that have been introduced (from filing an IND to market launch and beyond). (selexis.com)
To determine whether changes in PKCalpha and/or PKCbeta expression control retinoic acid (RA)- and dibutyryl cyclic AMP-induced F9 cell differentiation, we established cell lines stably expressing PKCalpha, PKCbeta, antisense PKCalpha, or antisense PKCbeta RNAs. (aacrjournals.org)
Under the Centinel™ program, Merck can modify customers' CHO cell lines to provide viral resistance to MVM. (merckmillipore.com)
Alternatively, customers can purchase the zinc finger nuclease pairs to engineer cell lines directly. (merckmillipore.com)
While previous efforts compared CHO cell lines by proteomics, research into the original Chinese hamster (Cricetulus griseus) host has not been conducted. (jhu.edu)
As glycosylation is critical for recombinant protein quality, we additionally performed a glycoproteomics and sialoproteomics analysis of wild-type and mutant CHO cell lines that differ in glycosylation capacity. (jhu.edu)
In conclusion, this large-scale proteomics analysis delineates specific changes across cell lines and tissues, which can help explain tissue function and the adaptations cells incur as biotherapeutics production hosts. (jhu.edu)

EX-CELL ® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. (sigmaaldrich.com)
EX-CELL ® Advanced™ CHO Fed-Batch Medium is formulated without L-glutamine and without hypoxanthine/thymidine. (sigmaaldrich.com)
Transfer the entire contents aseptically into a 125-mL shake flask containing 30 mL prewarmed complete EX-CELL ® Advanced TM CHO Fed-Batch Medium. (sigmaaldrich.com)
Pre-warm complete EX-CELL ® Advanced™ CHO Fed-Batch Medium to room temperature. (sigmaaldrich.com)
Determine the correct volume of cell culture to inoculate a new flask at a starting cell density of 2-3 x10 5 cells/ml in a total volume of 30 mL fresh EX-CELL ® Advanced™ CHO Fed-Batch Medium per 125-mL shake flask. (sigmaaldrich.com)
Prepare a freezing medium consisting of 46.5% cold EX-CELL ® Advanced™ CHO Fed-Batch Medium, 46.5% conditioned medium and 7% dimethyl sulfoxide (DMSO). (sigmaaldrich.com)
GE HyClone ActiPro medium and Cell Boost 7a and 7b supplements are designed and optimised for large-scale protein production in fed-batch suspension culture using recombinant CHO cells. (labonline.com.au)
ActiPro medium and Cell Boost 7a and 7b supplements can be used together in a fed-batch process or the medium and supplements can be used in combination with other products to increase productivity of an existing process. (labonline.com.au)
Clincke M-F, Guedon E, Yen FT, Ogier V, Goergen J-L (2011) Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced by CHO cells during batch processes. (springer.com)
The cumulative effect of these changes, however, did not negatively influence the ability to culture Chinese Hamster Ovary cells, as evaluated by cell viability, growth rate, and protein titer measurements in simple batch growth compared with the same cells cultured in control media exposed to visible light. (uwaterloo.ca)
Viable cell density (VCD), cell viability, and relative product concentration assayed during the fed-batch culture of CHO rDG44 cells. (miltenyibiotec.com)
Ma N, Ellet J, Okediadi C, Hermes P, McCormick E, Casnocha S. A single nutrient feed supports both chemically defined NS0 and CHO fed-batch processes: improved productivity and lactate metabolism. (springer.com)
Peak antibody production is associated with increased oxidative metabolism in an industrially relevant fed-batch CHO cell culture. (springer.com)
Available in powder and compacted forms, Cellvento® CHO formulations are chemically defined, of non-animal origin and lead to superior cell growth and productivity in batch, fed-batch mode or perfusion applications. (merckmillipore.com)
Cellvento® CHO media and companion feeds support consistent growth and performance in fed-batch culture. (merckmillipore.com)
It is a high concentrated feed that comes in compacted format and is compatible with all Cellvento® CHO fed-batch media. (merckmillipore.com)
Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. (semanticscholar.org)

Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i don't know how to optimize the conditions in this aspect. (protocol-online.org)
It is critical that the cumulative volume of the cell suspension added in this step and reagents added in step 5 does not exceed 10 mL. (stemcell.com)
Add the cell suspension in liquid growth medium to the semi-solid medium. (stemcell.com)
In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. (selectscience.net)

Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. (diva-portal.org)
In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. (diva-portal.org)
Here we describe the application of a closed-loop control scheme for the long-term cultivation of CHO cells in a high cell density (35 - 40 million cells/ml) perfusion process. (bioprocessintl.com)

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. (nexcelom.com)
Individual cells grow to form discrete, monoclonal colonies that are picked from the semi-solid medium after 10 - 14 days of incubation. (stemcell.com)
In our last situation, a major biopharmaceutical manufacturer incorporates CHO cell expression for monoclonal antibody production and purification, with filtration playing a major role in the production process. (thefreedictionary.com)
Dionne B, Mishra N, Butler M (2017) A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture. (springer.com)
In some cases this over-expression resulted in increased recombinant protein expression and enhanced cell specific productivity when transiently expressed in model cB72.3 monoclonal antibody producing cells. (bl.uk)

Also, cultured cells show diminished angiotensin II receptor binding with respect to time in culture and passage number. (springer.com)
To address these problems, we expressed the recombinant AT 1A receptor in CHO-K1 cells. (springer.com)
In AT 1A receptor transfected CHO-K1 cells, angiotensin II (10 −9 M) stimulated a rapid increase in cytosolic free calcium that was completely desensitized within 50 sec following receptor occupancy. (springer.com)
Receptor desensitization was also unaffected by inhibition or activation of protein kinase C. Thus, we have established a permanent, high-level transfectant of the AT 1A receptor in CHO-K1 cells and have shown that these receptors rapidly desensitize following exposure to physiological concentrations of agonist. (springer.com)
Targeted disruption of CD22 in mice results in a reduced level of surface IgM on peripheral B cells, suggesting a role for CD22 in limiting antigen receptor signaling. (biovendor.com)
Anthrax protective antigen interacts with a specific receptor on the surface of CHO-K1 cells. (asm.org)
Pretreatment of cells with a number of different proteases strongly inhibits PA binding, suggesting that the receptor may be at least partially proteinaceous. (asm.org)
Our results suggest that a cell surface protein(s) of 85 to 90 kDa is, or constitutes a portion of, a specific receptor for the PA. (asm.org)
On T cells, membrane-anchored LAG-3 is an inhibitory receptor downregulating T-cell receptor (TCR) signaling. (wikipedia.org)

In developing biological drugs, scientists often rely on Chinese hamster ovary (CHO) cells for early studies. (genengnews.com)
In this work, we describe a model-based platform for understanding the culture factors that affect recombinant protein glycosylation in Chinese hamster ovary (CHO) cells, the workhorse of the biopharmaceutical industry. (aiche.org)
Since serum is a major cost factor and an obstacle to protein purification, our goal was to develop a large-scale TGE process for Chinese hamster ovary (CHO) cells in the absence of serum. (epfl.ch)
In this study, we investigated the effect of nickel and cobalt on glycosylation of recombinant IgG expressed in Chinese hamster ovary cells. (springer.com)
Campbell C, Stanley P (1984) A dominant mutation to ricin resistance in Chinese hamster ovary cells induces UDP-GlcNAc: glycopeptide beta-4- N -acetylglucosaminyltransferase III activity. (springer.com)
The phenomenon of premature chromosome condensation, which involves the fusion of mitotic with interphase cells, was applied to the study of bleomycin-induced chromosome damage in Chinese hamster ovary cells. (aacrjournals.org)
Hence, Chinese hamster ovary cell (CHO) hepatitis B vaccine is considered to be the safe and effective method to prevent the hepatitis B infection. (pharmiweb.com)
However, inherent disadvantages of using recombinant Chinese hamster ovary cell (CHO) to design hepatitis B vaccines restrains the growth of the market. (pharmiweb.com)
The studies, led by scientists at Momenta Pharmaceuticals, demonstrate that Chinese Hamster Ovary (CHO) cells contain the active biosynthetic machinery to produce alpha-Gal antigens and that a therapeutic protein manufactured in CHO contains alpha-Gal. (nbcnews.com)
Differential Phosphoproteomic Analysis of Recombinant Chinese Hamster Ovary Cells Following Temperature Shift. (semanticscholar.org)
Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. (semanticscholar.org)
The aims of this study are to characterize the binding of E-1/E-2 and the subsequent effects on cyclic AMP formation and [Ca2+]i levels in SH-SY5Y and Chinese hamster ovary (CHO) cells expressing endogenous and recombinant mu-opioid receptors. (le.ac.uk)
Chinese hamster ovary (CHO) cells were isolated in the late 1950's and have been the workhorse of biotherapeutics production for decades. (jhu.edu)

Technologies and methods that result in the development and identification of robust and stable CHO pools and clones that can be paired with platform media, feed, and bioprocesses will be imperative for reducing timelines," says Oren Beske, amalgamator of business and biology, ATUM. (genengnews.com)
Overall, he points out that biopharma needs "CHO pools and clones that can rapidly be scaled up to the appropriate scale without process development and with a low-risk profile. (genengnews.com)
This application note compares cell growth and recombinant protein production of multiple CHO cell clones when cultured in GE HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements. (labonline.com.au)
Despite let-7 having a recognised role in deregulated cell growth no improvement was observed in stable, sponge-transfected clones. (dcu.ie)
The viable cell density (VCD) attained by different clones of CHO cells cultured in CHOMACS CD Medium in bioreactors. (miltenyibiotec.com)

The unaltered apoptotic rate supported the proposition that the increase in cell number was a result of enhance cell cycle kinetics and cellular metabolism rather than increasing viability. (doaj.org)
Studies have demonstrated that silencing certain target genes involved in certain CHO cell apoptotic and metabolic pathways resulted in 40 to 60% improved cell viability as compared with untreated cells. (thefreedictionary.com)
Previously, we have reported that SPD inhibited the proliferation of MCF-7 human breast cancer cells by inducing apoptotic cell death, while having minimal effects on non-malignant cells. (nih.gov)
To confirm that the SPD-induced apoptotic cell death was due to the involvement of caspase-7, cells were also treated with SPD in the presence of the specific inhibitor of caspase-7, Ac-DEVD-CHO [34]. (nih.gov)

Elucidating the role of copper in CHO cell energy metabolism using (13) C metabolic flux analysis. (springer.com)

Very high cell densities were achieved and could be stably maintained at high viability indicating of a healthy process suitable for instance for efficient cell banking. (diva-portal.org)

Note: If cell viability is below 90%, troubleshoot conditions prior to continuing. (sigmaaldrich.com)
Have you done a viability assay to ensure that the floating cells are, in fact, dead? (protocol-online.org)
Addition of a mixture of amino acids was found to improve cell viability, which got altered by high glucose in the CHO-K1 cells. (biomedsearch.com)
Cellvento® 4CHO-X has been designed to support high growth and viability, thus increasing cell biomass for cryopreservation or inoculation of another bioreactor. (merckmillipore.com)

CHO glycosylation mutants. (thermofisher.com)
Eight NSs and outlets for host cell protein N-linked and O-linked glycans as well as mAb glycosylation have been considered. (aiche.org)
Crowell CK, Grampp GE, Rogers GN, Miller J, Scheinman RI (2007) Amino acid and manganese supplementation modulates the glycosylation state of erythropoietin in a CHO culture system. (springer.com)

For example, a very different set of expertise would be required to operate a microbial fermenter than to operate a CHO cell bioreactor. (thefreedictionary.com)

CHO-Lec2 cells are mutants that have a 70-90% de- ficiency of sialic acid in their glycoproteins and gangliosides. (thermofisher.com)

Life Technologies Pledges $20M to Expand Cell Culture Capacity at Scottish. (genengnews.com)
The CHO cell is at its height of technological prominence thanks to its adaptability to various culture conditions and plasticity in the context of genetic alterations. (aiche.org)
Aseptically remove a small volume of cell culture sample from the flask and count by trypan blue exclusion using a hemocytometer or an automated cell counter. (sigmaaldrich.com)
Passage cells by repeating the above steps at least twice weekly, and expand culture volume as necessary. (sigmaaldrich.com)
A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros, Oregon Green 488, and Hoechst 33258. (microscopyu.com)
It is also known that the availability of the NSs is affected by process conditions including cell culture mode, temperature, dissolved oxygen tension, nutrient availability and addition of precursor molecules [1,2]. (aiche.org)
Specifically, the higher scale model is a cell culture dynamics (CCDyn) model, which describes cell growth and the variation of the nutrient and product concentrations over time in the extracellular medium. (aiche.org)
No cell culture is required prior to testing, for these frozen cells. (perkinelmer.com)
The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. (perkinelmer.com)
That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. (perkinelmer.com)
These cells are robust in culture and can produce a variety of recombinant glycoproteins at high levels in large scales. (labonline.com.au)
In the drug development timeline, cell culture medium and any associated supplements should be introduced and tested right from the R&D phase. (labonline.com.au)
Difficulties include restrictions on further modifying the cells, obligations regarding culture media use, constraints to which contract manufacturing organizations (CMO) can be used, and significant financial barriers associated with both upfront and downstream costs. (bioprocessintl.com)
Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. (creativebiomart.net)
The cells will re-attach to the surface over a period of several days in culture at 37°C. (creativebiomart.net)
1. Detach cells from culture dish according to the Sub-Culture Procedure.2. (creativebiomart.net)
Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. (creativebiomart.net)
Crotalus durissus terrificus venom and its components can affect a variety of cell types, including macrophages (9), neutrophils (34), mast cells (8), platelets (7) and cells in culture (14), as well as the function of organs such as the heart (19) and the kidney (22). (scielo.br)
Venom stock solutions (2.5mg/ml) were prepared in phosphate buffered saline (0.1M sodium phosphate, 0.15M NaCl), pH 7.2, sterilized using a 0.22µm filter (Millipore) and kept at 4°C. Crude venom solutions were diluted in cell culture medium before use. (scielo.br)
Our work in optimization of animal-cell culture is a hybrid approach between using basal media and very complete, chemically defined, and serum-free media. (bioprocessintl.com)
We followed the same logic with our CHO cell culture, with an objective function of cell growth. (bioprocessintl.com)
Sterility of cell culture media is an important concern in biotherapeutic processing. (uwaterloo.ca)
In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. (uwaterloo.ca)
This makes UV irradiation attractive for use in sterilization of cell culture media. (uwaterloo.ca)
The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. (uwaterloo.ca)
Here, we describe genetic approaches to improve CHO cell culture longevity, with a view to increasing overall process yield via the manipulation of two miRNAs: Let-7a and miR-7. (dcu.ie)
In the first approach, we used a Let-7 sponge decoy vector to deplete endogenous Let-7 levels with a view to increasing culture longevity and productivity of CHO-K1 SEAP expressing cells. (dcu.ie)
In addition, the effects of cell culture conditions on protein production in transiently transfected cells are illustrated. (selectscience.net)
In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. (physiciansweekly.com)
The method was successfully applied to analyze the extracts from CHO cells under two different culture conditions. (springer.com)
Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. (semanticscholar.org)
For more information about Thermo Scientific HyCell CHO cell-culture medium, to request a sample or to learn more about Thermo Scientific Cell Culture and Bioprocessing technologies, please visit www.thermoscientific.com/bioprocessing. (technologynetworks.com)
Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. (umassmed.edu)

As Weinguny notes, the cells "display a wide array of observed phenotypes. (genengnews.com)
Therefore, this stimulated the interest of our research group to manipulate these miRNAs in CHO cells with a view to positively impact bioprocess-relevant CHO cell phenotypes. (dcu.ie)
Application of C-13 flux analysis to identify high-productivity CHO metabolic phenotypes. (springer.com)

phase and to the uncoupling of cell size from cell proliferation. (doaj.org)
Determined by its ability to inhibit the proliferation of Raji cells. (biovendor.com)
The purpose of this study was to determine if PbTxs could induce chromosomal aberrations and inhibit cellular proliferation in CHO-K1-BH4 cells and if so could the damage be negated or reduced by the PbTx antagonist brevenal. (uncg.edu)
Results from the inhibition of cellular proliferation assays demonstrated that PbTxs inhibit the ability of CHOK1- BH4 cells to proliferate an effect which can be reduced with brevenal. (uncg.edu)

Cell density and protein production were shown to be comparable between scales. (labonline.com.au)

Human Recombinant, in CHO-K1 host cell. (perkinelmer.com)

In previous studies, we used two complementary two-dimensional gel electrophoretic methods to examine replication intermediates in the 240-kb amplified dihydrofolate reductase (DHFR) domain of methotrexate-resistant CHOC 400 cells (J. P. Vaughn, P. A. Dijkwel, and J. L. Hamlin, Cell 61:1075-1087, 1990). (asm.org)

Baser B, Spehr J, Bussow K, van den Heuvel J (2016) A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells. (springer.com)
Taken together, these data indicate that both adjuvant and cell-type expression can affect the elicitation of tier 2 and tier 1 neutralizing responses in vivo . (frontiersin.org)
CD22 is a B-lineage restricted 135 kDa glycoprotein whose cell surface expression is limited to resting and activated B lymphocytes. (biovendor.com)
Horizon licenses its CHO expression system to pharmaceutical, biotechnology, and biosimilar companies, as well as contract manufacturing organizations. (horizondiscovery.com)
Optimised protein expression protocols provide the ability to load cells with greater quantities of DNA relative to the standard CHO protocol. (selectscience.net)
As a result, average protein expression per cell is increased. (selectscience.net)
Finally, titers of soluble TNFαR are shown to increase for at least one week and are sustained for up to two weeks following electroporation of CHO cells with a sTNFαR expression plasmid. (selectscience.net)
As F9 embryonal carcinoma cells differentiate into parietal endoderm-like cells, expression of conventional protein kinase C (PKC) changes. (aacrjournals.org)
Constitutive expression of PKCalpha or inhibition of PKCbeta expression in F9 stem cells enhanced RA induced differentiation, both by increasing total expression and accelerating RA-induced expression of laminins A, B1, B2, and type IV collagen. (aacrjournals.org)
Expression of beta 1B integrin isoform in CHO cells results in a dominant negative effect on cell adhesion and motility. (rupress.org)
Expression of beta 1B also results in severe reduction of cell motility in the Boyden chamber assay. (rupress.org)

Gao Y et al (2016) Combined metabolomics and proteomics reveals hypoxia as a cause of lower productivity on scale-up to a 5000-liter CHO bioprocess. (springer.com)
In the second approach, we targeted a previously verified miRNA for improved CHO cell growth and productivity i.e. miR-7, using a bacterial genome-editing tool, CRISPR-Cas9. (dcu.ie)
Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. (semanticscholar.org)
HyCell™ CHO establishes a new benchmark for cell growth and productivity. (technologynetworks.com)
Its versatility allows quick adaptation and supports exceptional growth, high cell density and high productivity. (technologynetworks.com)

The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. (rupress.org)
Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25. (rupress.org)

Some literature suggests there are no functional potassium channels in wild type CHO and HEK cells although Frasermoss recently indicated some HEK cells have endogenous channels. (scientistsolutions.com)
The CHO-K1s I have used are great for hERG K recordings because they have no endogenous K currents. (scientistsolutions.com)
Our Membrane Target System membranes are prepared from cells that express recombinant or endogenous receptors. (neobits.com)
Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. (neobits.com)

In addition, BG505 NFL and BG505 SOSIP trimers expressed from 293F cells, when formulated in Adjuplex adjuvant, elicited equivalent BG505 tier 2 autologous neutralizing titers. (frontiersin.org)
These titers were lower in potency when compared to the titers elicited by CHO-M cell derived trimers. (frontiersin.org)

70% increase in maximal cell density without additional supply of nutrients the cells underwent an overall reduction of 14% in size as well as a significant decrease in glucose and amino acid consumption rate. (doaj.org)
Cell density could be significantly further increased showing the capacity of the system set-up. (diva-portal.org)

Resuspend the cells in liquid growth medium (such as ClonaCell™-CHO CD Liquid, Catalog #03817). (stemcell.com)
These data highlight some of our results in applying RNAi to augment CHO cell growth by targeting both cell death and metabolic pathways that are detrimental to the optimal performance of cells grown in bioreactors. (thefreedictionary.com)
Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. (creativebiomart.net)
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. (creativebiomart.net)
What is the market size in 2018 and the growth rate of the Global Recombinant Hamster Ovary Cell (CHO) Hepatitis B Vaccine Market? (pharmiweb.com)
Here, the proof-of-concept was quite positive and showed an increase in cell growth for the FreeStyle CHO medium. (bioprocessintl.com)
CHOMACS CD medium is a complete chemically-defined, animal component-free, protein-free, and ready-to-use media developed for long term, high performance growth and protein yield of CHO cells. (miltenyibiotec.com)
The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. (innoget.com)
In the initial assay without S9-mix, cell growth was essentially uneffected at Granola 97 concentrations up to and including 500 g/mL, growth was reduced to 72% compared to the solvent control at 1500 g/mL and reduced to 0% at 5000 g/mL. (epa.gov)
In the presence of S9-mix, cell growth was 55% of the solvent control at 1500 g/mL and 0% at 5000 g/mL. (epa.gov)
In the repeat assay after 20 hours exposure to Granola 97 without S9-mix, cell growth remained above 50% of the solvent control value at all concentrations except 3000 g/mL where growth was 4% of the solvent control. (epa.gov)
After 44 hours exposure, cell growth remained above 50% of the solvent control value at concentrations up to and including 500 g/mL and was reduced to 42%, 18% and 0% at 1000, 1500 and 3000 g/mL, respectively. (epa.gov)
In the presence of S9-mix (6 hour exposure), cell growth remained above 50% of the solvent control value at all concentrations except 3000 g/mL where growth was 3% of the solvent control. (epa.gov)

We demonstrate that CRISPR-Cas9 can be successfully used to target miRNA loci in the CHO genome but that functional knockout may be more difficult compared to protein coding genes. (dcu.ie)

In addition, MC2R-CRE-bla-CHO-K1 cells have been tested for assay performance under variable conditions. (caigou.com.cn)
Results from the chromosomal aberrations assay demonstrated that PbTxs are potent inducers of CHO-K1-BH4 chromosome damage. (uncg.edu)
In the initial assay without S9-mix, no statistically significant increase (p# 0.05) over the solvent control was seen in the percentage of cells with structural or numerical aberrations at any tested concentrations of Granola 97. (epa.gov)

The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. (scielo.br)
The aim of the present work was, therefore, to determine the effect of crotalid venom on CHO-K1 cells, particularly its action on actin filaments, endoplasmic reticulum and nucleus. (scielo.br)

55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. (epfl.ch)
However, microarray studies also implicated additional transcriptional targets in GSI-I-dependent cell death, including genes in the unfolded protein response, nuclear factor-κB and p53 pathways. (pubmedcentralcanada.ca)

Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. (perkinelmer.com)
FroZen Cells validated for calcium testing: GPR99. (perkinelmer.com)
Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. (perkinelmer.com)
E-1/E-2 failed to increase intracellular calcium in CHOdelta, CHOkappa and SH-SY5Y cells. (le.ac.uk)

Providing our customers with innovative bioanalytical solutions for protein and cell biology for over 30 years. (moleculardevices.com)
To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)

Anatomy7

Organisms3

Chemicals and Drugs23

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes13

Disciplines and Occupations1

Information Science3

Health Care1

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

Proteoglycan involvement in polyamine uptake. (4/15188)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

CHO Cells

Cricetinae

Cricetulus

Transfection

Ovary

Molecular Sequence Data

Cell Line

Amino Acid Sequence

Glycosylation

Cell Membrane

Cloning, Molecular

Base Sequence

Mutation

Recombinant Fusion Proteins

Kinetics

DNA, Complementary

Radioligand Assay

Endocytosis

Adenine Phosphoribosyltransferase

Cyclic AMP

Binding, Competitive

Receptors, Virus

Gene Expression

Protein Binding

Biological Transport

Choline

Diprenorphine

Cell Adhesion

DNA

Guanosine 5'-O-(3-Thiotriphosphate)

Tetrahydrofolate Dehydrogenase

Creatine

Protein Processing, Post-Translational

Polysaccharides

Membrane Glycoproteins

Membrane Proteins

Cell Line, Tumor

Lateral Line System

Longitudinal Studies

Motilin

Receptors, Gastrointestinal Hormone

Receptors, Neuropeptide

Erythromycin

Gastrointestinal Agents

Cisapride

Pyloric Antrum

Cynanchum

Pregnenes

Biopharmaceutics

Recombinant Proteins

Protein Engineering

Culture Media

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

Proteoglycan involvement in polyamine uptake. (4/15188)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

AID 41661 - Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with...

中国科学院大连化学物理研究所机构知识库(DICP OpenIR): Tumor anti-angiogenic gene therapy with microencapsulated recombinant CHO cells

Neutralization Of Agglutination On Chinese Hamster Ovary Cells By Pertussigen

AID 34248 - Displacement of [3H]NECA from human adenosine A2A receptor in stably transfected CHO cells - PubChem

Adhering CHO cell culture - Tissue and Cell Culture - BioForum

serumm free culture of adherent cho cells - Tissue and Cell Culture - BioForum

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells - Infoscience

Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and...

Horizon Discovery signs license agreement with Glenmark Pharmaceuticals for GS knockout CHO cell line for manufacture of...

CHO Cell Lines Need Checking

A protective role for HIF-1 in response to redox manipulation and glucose deprivation: implications for tumorigenesis. -...

A protective role for HIF-1 in response to redox manipulation and glucose deprivation: implications for tumorigenesis.

Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells | Sigma-Aldrich

YUHSpace: Cho, Eun Suk

Publications - EQUIPMENT BOKU VIENNA INSTITUTE OF BIOTECHNOLOGY GMBH

Proteolytic processing of PA triggers partitioning of t | Open-i

Clearing the Fog Around Aβ Oligomers | ALZFORUM

The conditioned medium from a stable human GDF3-expressing CHO cell line, induces the differentiation of PC12 cells. | Sigma...

CHO Cell Line Engineering and Design - Activities - DTU Research Database

MEMORY BASED UP CHO QUESTION PAPER||UP CHO ANSWER KEY 2021 SHIFT FIRST||UP CHO 10/9/21 MCQ| SHIFT 1