Cho cells. Medical search

CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Established cell cultures that have the potential to propagate indefinitely.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The rate dynamics in chemical or physical systems.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).

Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

An enzyme catalyzing the formation of AMP from adenine and phosphoribosylpyrophosphate. It can act as a salvage enzyme for recycling of adenine into nucleic acids. EC 2.4.2.7.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.

Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.

A narcotic antagonist similar in action to NALOXONE. It is used to remobilize animals after ETORPHINE neuroleptanalgesia and is considered a specific antagonist to etorphine.

Adherence of cells to surfaces or to other cells.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.

An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3.

An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

Glycoproteins found on the membrane or surface of cells.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

In this study the direct involvement of cadherins in adhesion-mediated growth inhibition was investigated. It is shown here that overexpression of N-cadherin in CHO cells significantly suppresses their growth rate. Interaction of these cells and two additional fibroblastic lines with synthetic beads coated with N-cadherin ligands (recombinant N-cadherin ectodomain or specific antibodies) leads to growth arrest at the G1 phase of the cell cycle. The cadherin-reactive beads inhibit the entry into S phase and the reduction in the levels of cyclin-dependent kinase (cdk) inhibitors p21 and p27, following serum-stimulation of starved cells. In exponentially growing cells these beads induce G1 arrest accompanied by elevation in p27 only. We propose that cadherin-mediated signaling is involved in contact inhibition of growth by inducing cell cycle arrest at the G1 phase and elevation of p27 levels. (+info)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

The DNA repair protein apurinic endonuclease (APE/Ref-1) exerts several physiological functions such as cleavage of apurinic/apyrimidinic sites and redox regulation of the transcription factor AP-1, whose activation is part of the cellular response to DNA damaging treatments. Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towards AP-1, thus promoting DNA binding activity of AP-1. Inhibition of CKII mediated phosphorylation of APE/Ref-1 blocked mutagen-stimulated increase in AP-1 binding. It also abrogated the induction of c-Jun protein and rendered cells more sensitive to induced DNA damage. Thus, phosphorylation of APE/Ref-1 appears to be involved in regulating the different physiological activities of the enzyme. CKII mediated phosphorylation of APE/Ref-1 and concomitant increase in AP-1 binding activity appears to be a novel mechanism of cellular stress response, forcing transcription of AP-1 target gene(s) the product(s) of which may exert protective function. (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located. (+info)

Proteoglycan involvement in polyamine uptake. (4/15188)

We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake considerably, whereas chondroitin sulphate lyase had a limited effect. Inhibition of polyamine biosynthesis with alpha-difluoromethylornithine resulted in an increase of cell-associated heparan sulphate proteoglycans exhibiting higher affinity for spermine. The data indicate a specific role for heparan sulphate proteoglycans in the uptake of spermine by fibroblasts. Spermine uptake by pgsD-677, a mutant Chinese hamster ovary cell defective in heparan sulphate biosynthesis, was only moderately reduced (20%) compared with wild-type cells. Treatment of mutant cells with the above-mentioned xyloside resulted in a greater reduction of endogenous proteoglycan production as well as a higher inhibition of spermine uptake than in wild-type cells. Moreover, treatment with chondroitin sulphate lyase resulted in a selective inhibition of uptake in mutant cells, indicating a role for chondroitin/dermatan sulphate proteoglycans in the uptake of spermine by these cells. Fibroblasts, made growth-dependent on exogenous spermine by alpha-difluoromethylornithine treatment, were growth-inhibited by heparan sulphate or beta-D-xyloside, which might have future therapeutical implications. (+info)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Serum-free culture filtrates of six Candida species and Saccharomyces cerevisiae were found to contain chemoattractants for human polymorphonuclear leukocytes (PMNs) and a mouse macrophage-like cell line, J774. The chemotactic factors differed for the PMN and J774 cells, however, in terms of heat stability, kinetics of liberation by the yeast cells, and divalent cation requirements for production. The chemoattractant in Candida albicans culture filtrates appeared to act through the formyl peptide receptor (FPR) of PMNs, since it was found to induce chemotaxis of Chinese hamster ovary (CHO) cells that were expressing the human FPR but did not induce chemotaxis of wild-type CHO cells. The C. albicans culture filtrates also induced migration of PMNs across confluent monolayers of a human gastrointestinal epithelial cell line, T84; migration occurred in the basolateral-to-apical direction but not the reverse direction, unless the epithelial tight junctions were disrupted. J774 cells did not migrate toward the formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albicans culture filtrate was not inhibited by an FPR antagonist (t-butoxycarbonyl-Met-Leu-Phe), suggesting that a different receptor mediated J774 cell chemotaxis. In conclusion, we have identified a receptor by which a non-serum-dependent chemotactic factor (NSCF) produced by C. albicans induced chemotaxis of PMNs. Additionally, we have shown that NSCF was active across epithelial monolayers. These findings suggest that NSCFs produced by C. albicans and other yeast species may influence host-pathogen interactions at the gastrointestinal tract mucosal surface by inducing phagocytic-cell infiltration. (+info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology. (+info)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

A genetic locus for a cytolethal distending toxin (CDT) was identified in a polymorphic region of the chromosome of Actinobacillus actinomycetemcomitans, a predominant oral pathogen. The locus was comprised of three open reading frames (ORFs) that had significant amino acid sequence similarity and more than 90% sequence identity to the cdtABC genes of some pathogenic Escherichia coli strains and Haemophilus ducreyi, respectively. Sonic extracts from recombinant E. coli, containing the A. actinomycetemcomitans ORFs, caused the distension and killing of Chinese hamster ovary cells characteristic of a CDT. Monoclonal antibodies made reactive with the CdtA, CdtB, and CdtC proteins of H. ducreyi recognized the corresponding gene products from the recombinant strain. CDT-like activities were no longer expressed by the recombinant strain when an OmegaKan-2 interposon was inserted into the cdtA and cdtB genes. Expression of the CDT-like activities in A. actinomycetemcomitans was strain specific. Naturally occurring expression-negative strains had large deletions within the region of the cdt locus. The cdtABC genes were flanked by an ORF (virulence plasmid protein), a partial ORF (integrase), and DNA sequences (bacteriophage integration site) characteristic of virulence-associated regions. These results provide evidence for a functional CDT in a human oral pathogen. (+info)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein. (+info)

The iCapTag™-target protein complex can be expressed in a wide range of expression hosts (e.g. CHO and E.coli cells). It is not ... Zhang, Jin; Campbell, Robert; Ting, Alice; Tsien, Roger (2002). "Creating new fluorescent probes for cell biology". Nat Rev Mol ... Zhang, Jin; Campbell, Robert; Ting, Alice; Tsien, Roger (2002). "Creating new fluorescent probes for cell biology". Nat Rev Mol ... Cell Chemical Biology. 29 (2): 339-350.e10. doi:10.1016/j.chembiol.2021.07.005. ISSN 2451-9456. PMC 8878318. PMID 34324879. Tan ...

https://en.wikipedia.org/wiki/Protein_tag

It is produced in CHO cells. In September 2020, phase I clinical trials of UB-612 started in Taiwan. and in January 2021, phase ... May 2022). "A multitope SARS-CoV-2 vaccine provides long-lasting B cell and T cell immunity against Delta and Omicron variants ... UB-612 provides B-cell and T-cell memory immunity and offers a potential as a universal vaccine to fend off the Omicron variant ... It is composed of SARS-CoV-2 S1-RBD protein and synthetic peptides representing T cell (Th and CTL) epitopes on the ...

https://en.wikipedia.org/wiki/UB-612

Efti is manufactured in CHO cells. Immutep worked with Henogen as the contracted manufacturing organization to provide efti for ... Activated T cells. The eight patients experienced sustained CD8+ T-cell activation (as measured by percentage of CD8+ T cells ... Increase in relevant cell numbers. There was a sustained increase in the number of monocytes, NK cells, and activated CD8+ T ... Cell Genesys' lead product, GVAX, consisted of whole tumor cells genetically modified to secrete GM-CSF. When mice that had ...

https://en.wikipedia.org/wiki/Eftilagimod_alpha

... it is manufactured in CHO cells. As of 2016, it was in phase III studies for induction and maintenance therapy in people with ... Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ... "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ...

https://en.wikipedia.org/wiki/Etrolizumab

It is manufactured recombinantly in CHO cells. The monoclonal antibody 2C4 appears to have first been published in 1990 by ... sets off signal transduction through several pathways that stimulate cell proliferation and cell growth; if overexpressed it ... Badache A, Hynes NE (April 2004). "A new therapeutic antibody masks ErbB2 to its partners" (PDF). Cancer Cell. 5 (4): 299-301. ... In both uses, more than 10% of people additionally experienced: loss of red blood cells, hypersensitivity or allergic reaction ...

https://en.wikipedia.org/wiki/Pertuzumab

It is recombinantly manufactured in Chinese hamster ovary (CHO) cells. Pembrolizumab was invented by scientists at Organon ... Normally, the PD-1 receptor on activated T-cells binds to the PD-L1 or PD-L2 ligands present on normal cells in the body, ... It works by targeting the cellular pathway of proteins found on the body's immune cells and some cancer cells, known as PD-1/PD ... deactivating any potential cell-mediated immune response against these cells. Many cancers make proteins such as PD-L1 that ...

https://en.wikipedia.org/wiki/Pembrolizumab

"Phase I Trial of a Recombinant COVID-19 Vaccine (CHO Cell)". ClinicalTrials.gov. United States National Library of Medicine. 19 ... "Efficacy, safety and immunogenicity of a recombinant protein subunit vaccine (CHO cells) against COVID-19 in adults: an ... "Phase II Clinical Trial of Recombinant SARS-CoV-2 Spike Protein Vaccine (CHO Cell)". ClinicalTrials.gov. 4 August 2021. ... "Phase I Clinical Trial of Recombinant SARS-CoV-2 Spike Protein Vaccine (CHO Cell)". ClinicalTrials.gov. 4 July 2021. ...

https://en.wikipedia.org/wiki/COVID-19_vaccine_clinical_research

The FDA has approved ERTs that are derived from other human cells, animal cells (i.e. Chinese hamster ovary cells, or CHO cells ... These cells have the ability to mature into the many cell types that comprise blood, including red blood cells, platelets, and ... Hematopoietic stem cell (HSC) transplantation is another treatment for lysosomal storage diseases. HSCs are derived from bone- ... Once inside the body the vector introduces the therapeutic gene into host cells, and the protein encoded by the newly inserted ...

https://en.wikipedia.org/wiki/Enzyme_replacement_therapy

"Phleomycin resistance as a dominant selectable marker in CHO cells". Somatic Cell and Molecular Genetics. 14 (3): 243-52. doi: ... and animal cells. It causes cell death by intercalating into DNA and inducing double stranded breaks of the DNA. Zeocin is blue ... When Zeocin enters a cell, the Cu2+ is reduced to Cu+ and then removed. Subsequently, Zeocin becomes activated and can then ... especially for the selection of eukaryotic cell lines when used in conjunction with vectors containing a selectable marker for ...

https://en.wikipedia.org/wiki/Zeocin

Thielemans L, Depoortere I, Vanden Broeck J, Peeters TL (2002). "The motilin pharmacophore in CHO cells expressing the human ... 2001). "Demonstration of a functional motilin receptor in TE671 cells from human cerebellum". Brain Res. 895 (1-2): 119-28. doi ...

https://en.wikipedia.org/wiki/Motilin_receptor

Yokomizo T, Noiri E, Izumi T, Shimizu T (2003). "In vivo chemotaxis using CHO cells expressing human leukotriene B4 receptor". ... Kato K, Yokomizo T, Izumi T, Shimizu T (2000). "Cell-specific transcriptional regulation of human leukotriene B(4) receptor ... "Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells". J Biol Chem. 271 (31): 18363-7. doi:10.1074/jbc ... "Leukotriene B4 receptor antagonist LY293111 inhibits proliferation and induces apoptosis in human pancreatic cancer cells". ...

https://en.wikipedia.org/wiki/Leukotriene_B4_receptor_1

Gieseler, A (2013). "Cell Membrane Toponomics". "Cell Membrane Toponomics" in Dubitzky, Wolkenhauer, Cho, Yokota. Encyclopedia ... "3D all-organelle real time visualization of a single cell" "Visualizing the protein-DNA network code inside the cell nucleus" ... Detection of 2000 Cell Surface Protein Clusters in a Single Tissue Section and Cell Type Specific Annotation by Using a Three ... detection of CD8+ CD18+ cells and CD8+ CD103+ cells by multi-epitope imaging". Clinical and Experimental Immunology. 112 (1): ...

https://en.wikipedia.org/wiki/Imaging_cycler_microscopy

The DNA was damaged in CHO cells as well as in mice. In mice (given 20 mg nivalenol /kg bw orally or 3.7 mg /kg bw ip) the DNA ... CHO) cells by slightly increased frequencies of chromosomal aberrations and sister chromatid exchange. ... NF-κB is a transcription factor that can be found in almost all human cells, and regulates the expression of its target genes ... Again, this effect is reduced by NF-κB inhibition which shows, that nivalenol and NF-κB interact to influence the cell. It was ...

https://en.wikipedia.org/wiki/Nivalenol

The clastogen-suppressing effects of Tochu tea in CHO cells and mice". Mutation Research. 388 (1): 7-20. doi:10.1016/s1383-5718 ... inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line". Cancer Research. 61 (15): 5749-56. ...

https://en.wikipedia.org/wiki/Deacetylasperulosidic_acid

Three primary expression systems have been established: EBNA-1-engineered CHO cell line CHO EBNA LT cell line, which is carried ... Recently, however, attempts to engineer CHO cells for transient protein production have garnered recognition. CHO cells were ... Codamo J, Munro TP, Hughes BS, Song M, Gray PP (June 2011). "Enhanced CHO cell-based transient gene expression with the epi-CHO ... Traditionally, Chinese hamster ovary (CHO) cells are associated with the establishment of stable cell lines for biologics. ...

https://en.wikipedia.org/wiki/Transient_expression

Skalski M, Coppolino MG (Oct 2005). "SNARE-mediated trafficking of alpha5beta1 integrin is required for spreading in CHO cells ... Bves demonstrates dynamic localization, dependent upon cell-cell junction formation. Prior to cell-cell contact, Bves is ... Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho ... Grossly, cell motility and cell adhesion are impaired. Only recently have the molecular mechanisms underlying the function of ...

https://en.wikipedia.org/wiki/Blood_vessel_epicardial_substance

"Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells". Mol. Pharmacol. 35 (4): 469-76. ...

https://en.wikipedia.org/wiki/Otenzepad

"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ... "Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells". Mol. Pharmacol. 35 (4): 469-76. ... Acetylcholine (often abbreviated ACh) is a neurotransmitter whose receptors are proteins found in synapses and other cell ... "Decreased binding affinity of olanzapine and clozapine for human muscarinic receptors in intact clonal cells in physiological ...

https://en.wikipedia.org/wiki/Muscarinic_antagonist

2015-03-04). "Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells". mAbs. 7 (2): 403-12. ... Therefore, up to 50% of F2A-linked proteins can remain in the cell as a fusion protein, which might cause some unpredictable ... 2011-04-29). Thiel V (ed.). "High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, ... April 2019). "Fusion of Reprogramming Factors Alters the Trajectory of Somatic Lineage Conversion". Cell Reports. 27 (1): 30-39 ...

https://en.wikipedia.org/wiki/2A_self-cleaving_peptides

This was followed by production of clinical grade alpha-glucosidase in Chinese hamster ovary (CHO) cells and in the milk of ... Genzyme also had its own product (Myozyme) grown in CHO cells under development. In November 2001, Genzyme chief executive ... muscle cells) or in white blood cells. The choice of sample depends on the facilities available at the diagnostic laboratory.[ ... Administration of the enzyme lead to its uptake by the liver and not the muscle cells where it is needed. In the early 1990s ...

https://en.wikipedia.org/wiki/Glycogen_storage_disease_type_II

"Metastin suppresses the motility and growth of CHO cells transfected with its receptor". Biochemical and Biophysical Research ... "Transcriptional expression of genes involved in cell invasion and migration by normal and tumoral trophoblast cells". The ... Journal of Cell Science. 117 (Pt 8): 1319-28. doi:10.1242/jcs.00971. PMID 15020672. "KiSS1-Derived Peptide Receptors". IUPHAR ... "Metastin receptor is overexpressed in papillary thyroid cancer and activates MAP kinase in thyroid cancer cells". The Journal ...

https://en.wikipedia.org/wiki/KiSS1-derived_peptide_receptor

2004). "Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells". Biochemistry. 43 ( ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3 PDB: 3NK4 Jovine L, Qi H, ... Cell. 143 (3): 404-15. doi:10.1016/j.cell.2010.09.041. PMID 20970175. S2CID 18583237. PDB: 3NK3, 3NK4 Bansal P, Chakrabarti K ... Cell Biol. 4 (6): 457-61. doi:10.1038/ncb802. PMID 12021773. S2CID 11575790. Jovine L, Qi H, Williams Z, Litscher ES, Wassarman ...

https://en.wikipedia.org/wiki/ZP3

"Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein". Cell. 87 (3): 415-26. doi: ... a possible trigger for degradation of HMG CoA reductase and crystalloid endoplasmic reticulum in UT-1 cells". Cell. 36 (4): 835 ... Sakai J, Duncan EA, Rawson RB, Hua X, Brown MS, Goldstein JL (Jun 1996). "Sterol-regulated release of SREBP-2 from cell ... Nov 1984). "The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA". Cell. 39 (1): 27-38. doi: ...

https://en.wikipedia.org/wiki/Michael_Stuart_Brown

"Benzodiazepines differently modulate EAAT1/GLAST and EAAT2/GLT1 glutamate transporters expressed in CHO cells". Neurochemistry ... Szymocha R, Akaoka H, Dutuit M, Malcus C, Didier-Bazes M, Belin MF, Giraudon P (July 2000). "Human T-cell lymphotropic virus ... In the retina, EAAT1 is expressed in Muller cells. EAAT1 is also expressed in a number of other tissues including cardiac ... The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1". DNA ...

https://en.wikipedia.org/wiki/Excitatory_amino_acid_transporter_1

"Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein". Cell. 87 (3): 415-26. doi: ... In cholesterol-depleted cells, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their ... V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1". DNA ... Brown MS, Goldstein JL (1999). "A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood". ...

https://en.wikipedia.org/wiki/SREBP_cleavage-activating_protein

"Benzodiazepines differently modulate EAAT1/GLAST and EAAT2/GLT1 glutamate transporters expressed in CHO cells". Neurochem. Int ... Cell. Neurosci. 19 (2): 152-64. doi:10.1006/mcne.2001.1066. PMID 11860269. S2CID 45768895. Reye P, Sullivan R, Fletcher EL, Pow ... J. Physiol., Cell Physiol. 281 (6): C1964-70. doi:10.1152/ajpcell.2001.281.6.C1964. PMID 11698255. S2CID 3173417. Tozaki H, ... Cell. Neurosci. 19 (2): 152-64. doi:10.1006/mcne.2001.1066. PMID 11860269. S2CID 45768895. Fontana AC (June 20, 2015). "Current ...

https://en.wikipedia.org/wiki/Excitatory_amino_acid_transporter_2

Giraudo CG, Rosales Fritz VM, Maccioni HJ (1999). "GA2/GM2/GD2 synthase localizes to the trans-golgi network of CHO-K1 cells". ... Cell. Biochem. 298 (1-2): 59-68. doi:10.1007/s11010-006-9350-0. hdl:11383/1672116. PMID 17119850. S2CID 29889795. v t e ( ...

https://en.wikipedia.org/wiki/B4GALNT1

"Agonist-induced internalization and desensitization of the human nociceptin receptor expressed in CHO cells". Cellular and ... "The effect of nociceptin on Ca2+ channel current and intracellular Ca2+ in the SH-SY5Y human neuroblastoma cell line". British ... "Activation of phospholipase A2 by the nociceptin receptor expressed in Chinese hamster ovary cells". Journal of Neurochemistry ... receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines". Brain Research. Molecular Brain ...

https://en.wikipedia.org/wiki/Nociceptin_receptor

2000). "Functional characterization of the NCC27 nuclear protein in stable transfected CHO-K1 cells". FASEB J. 14 (9): 1171-8. ... Cell. 11 (5): 1509-21. doi:10.1091/mbc.11.5.1509. PMC 14863. PMID 10793131. Tonini R, Ferroni A, Valenzuela SM, et al. ( ... Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and ... 2001). "The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle". J. Physiol. 529 (3): 541-52. doi: ...

https://en.wikipedia.org/wiki/CLIC1

Commonly used cell lines are Chinese hamster ovary (CHO) cells or plant cell cultures. The production volumes are very small. ... Cell Cultures Animal or plant cells, removed from tissues, will continue to grow if cultivated under the appropriate nutrients ... The inherent risks of the mammalian cell technology led several companies to opt out of mammalian cell technology or to ... When carried out outside the natural habitat, the process is called cell culture. Mammalian cell culture fermentation, also ...

https://en.wikipedia.org/wiki/Fine_chemical

Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, Walsh TR (December 2009). "Characterization of a new metallo-beta- ... a class of beta-lactam antibiotics that are capable of killing most bacteria by inhibiting the synthesis of one of their cell ...

https://en.wikipedia.org/wiki/New_Delhi_metallo-beta-lactamase_1

Cho C, Kim DH, Jung YK (2004). "Calcium binding of ARC mediates regulation of caspase 8 and cell death". Mol. Cell. Biol. 24 ( ... Cell. 15 (6): 901-12. doi:10.1016/j.molcel.2004.08.020. PMID 15383280. Jo DG, Jun JI, Chang JW, Hong YM, Song S, Cho DH, Shim ... and causes cell death in cultured cells". J. Biol. Chem. 275 (4): 2647-53. doi:10.1074/jbc.275.4.2647. PMID 10644725. Li PF, Li ... J. Physiol., Cell Physiol. 284 (6): C1405-10. doi:10.1152/ajpcell.00279.2002. PMID 12734105. Nam YJ, Mani K, Ashton AW, Peng CF ...

https://en.wikipedia.org/wiki/NOL3

The S-layer is made of a cell-surface glycoprotein that accounts for approximately 50% of the cell surface proteins. These ... Park, J. S.; Vreeland, R. H.; Cho, B. C.; Lowenstein, T. K.; Timofeeff, M. N. & Rosenweig, W. D. (December 2009). "Haloarchaeal ... The primary role of bacterioruberin in the cell is to protect against DNA damage incurred by UV light. This protection is not, ... It is able to protect the cell from reactive oxygen species produced from exposure to UV by acting as a target. The ...

https://en.wikipedia.org/wiki/Halobacterium_salinarum

Lee WS, Chun HK, Lee WY, Yun SH, Cho YB, Yun HR, et al. (September 2007). "Treatment outcomes in patients with signet ring cell ... Some cases are inherited, and these cases are often caused by mutations in the CDH1 gene, which encodes the important cell-cell ... As a result, the ErbB2/ErbB3 signaling pathway becomes constitutively activated, cell-cell interactions are lost and signet ... July 2013). "Signet ring cell colorectal carcinoma: a distinct subset of mucin-poor microsatellite-stable signet ring cell ...

https://en.wikipedia.org/wiki/Signet_ring_cell_carcinoma

Molecular Cell Biology. 20 (11): 665-680. doi:10.1038/s41580-019-0133-3. PMID 31253954. S2CID 195739183. Kishor A, White EJ, ... Liu J, Xia J, Cho KH, Clapham DE, Ren D (June 2007). "CatSperbeta, a novel transmembrane protein in the CatSper channel complex ... Liu J, Xia J, Cho KH, Clapham DE, Ren D (June 2007). "CatSperbeta, a novel transmembrane protein in the CatSper channel complex ... Hsp70-2 specifically is developmentally expressed in male germ line cells during meiosis, where it is necessary for the ...

https://en.wikipedia.org/wiki/HSPA1B

... cell to allow cell-to-cell transmission. As viral synapses allow the virus to spread directly from cell to cell, they also ... Cho MW, Dustin ML, Hioe CE (2008). "Human immunodeficiency virus type 1 envelope gp120 induces a stop signal and virological ... "Cell-to-Cell Transmission of HIV-1 Is Required to Trigger Pyroptotic Death of Lymphoid-Tissue-Derived CD4 T Cells". Cell Rep. ... of CD4 T cells leading to CD4 T-cell depletion and progression to AIDS are infected cells (not cell-free viral particles) ...

https://en.wikipedia.org/wiki/Viral_synapse

"The dynamic organization of the perinucleolar compartment in the cell nucleus". The Journal of Cell Biology. 137 (5): 965-974. ... Hahm B, Cho OH, Kim JE, Kim YK, Kim JH, Oh YL, Jang SK (April 1998). "Polypyrimidine tract-binding protein interacts with HnRNP ... Hahm B, Cho OH, Kim JE, Kim YK, Kim JH, Oh YL, Jang SK (April 1998). "Polypyrimidine tract-binding protein interacts with HnRNP ... Molecular Cell. 27 (3): 435-448. doi:10.1016/j.molcel.2007.07.015. PMC 3139456. PMID 17679093. Rasmussen HH, van Damme J, Puype ...

https://en.wikipedia.org/wiki/PTBP1

After Volta's discovery of the electrochemical cell in 1800, the term was then applied to a group of electrochemical cells " ... ISBN 0-8247-8498-7. Yasuo Cho (2005). Scanning Nonlinear Dielectric Microscope (PDF) (in Polar Oxides; R. Waser, U. Böttger & S ... In dynamic random-access memory (DRAM), each memory cell typically consists of a MOSFET and MOS capacitor. A capacitor consists ... subsequently applied to clusters of electrochemical cells. Leyden jars were later made by coating the inside and outside of ...

https://en.wikipedia.org/wiki/Capacitor

Chung JY, Cho JY, Lim HS, Kim JR, Yu KS, Lim KS, Shin SG, Jang IJ (January 2011). "Effects of pregnane X receptor (NR1I2) and ... in CYP2B6 and CYP2C19 and survival of patients receiving cyclophosphamide prior to myeloablative hematopoietic stem cell ...

https://en.wikipedia.org/wiki/CYP2B6

Kafi, R.; Kwak, H. S.; Schumacher, W. E.; Cho, S.; Hanft, V. N.; Hamilton, T. A.; King, A. L.; Neal, J. D.; Varani, J.; Fisher ... These help to dissolve the intracellular "glue" that holds dead cells together on the skin. The use of this type of product on ... Coenzyme Q10 Anti-oxidants are substances that may protect cells from the damage caused by unstable molecules known as free ... This exposes newer skin cells and can help improve appearance. AHAs may irritate some skin, causing redness and flaking.[ ...

https://en.wikipedia.org/wiki/Anti-aging_product

... the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell ... Cell. 136 (2): 272-83. doi:10.1016/j.cell.2008.11.047. PMC 2859625. PMID 19167329. Lizak C, Gerber S, Numao S, Aebi M, Locher ... Cell Biol. 161 (4): 715-25. doi:10.1083/jcb.200301043. PMC 2199356. PMID 12756234. Karaoglu D, Kelleher DJ, Gilmore R (October ...

https://en.wikipedia.org/wiki/Oligosaccharyltransferase

The Helicarrier also functioned as a headquarters and prison equipped with specially designed cells for supervillains. In the ... in an attempt to neutralize an attack by Amadeus Cho in Incredible Hercules #115. Black Hawk - Destroyed in action against a ...

https://en.wikipedia.org/wiki/Helicarrier

Gianneschi, Nathan C.; Cho, So-Hye; Nguyen, SonBinh T.; Mirkin, Chad A. (2004). "Reversibly Addressing an Allosteric Catalyst ... programmed interactions with biomolecules and cells, and basic research into nanoscale materials design, synthesis and ...

https://en.wikipedia.org/wiki/Nathan_C._Gianneschi

The poll asked "Where did human beings come from - did we start as singular cells millions of year ago and evolve into our ... Park, Hyung Wook; Cho, Kyuhoon (2018). "Science, state, and spirituality: Stories of four creationists in South Korea". History ... Klotzko, Arlene Judith (May 28, 2001). "Cynical Science and Stem Cells". The Scientist. 15 (11): 35. ISSN 0890-3670. Retrieved ...

https://en.wikipedia.org/wiki/Creationism

In 1974, he joined the teaching staff at Saigon University, and the following year became the director of Cho-Ray Hospital. He ... Que demanded improvement in the treatment of political prisoners, he was incarcerated in a five-by-six foot cell without ...

https://en.wikipedia.org/wiki/Nguyễn_Đan_Quế

February 12 - President Bush announces the nomination of Catherine Yi-yu Cho Woo for membership on the National Council on the ... April 13 - President Bush delivers an address in the fuel cell hangar at Maxwell Air Force Base War College in Montgomery, ... Nomination of Catherine Yi-yu Cho Woo To Be a Member of the National Council on the Arts (February 12, 1991) Nomination of John ...

https://en.wikipedia.org/wiki/Timeline_of_the_George_H._W._Bush_presidency_(1991)

Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Pollak ... Lee SH, Seo HY, Kim JC, Heo WD, Chung WS, Lee KJ, Kim MC, Cheong YH, Choi JY, Lim CO, Cho MJ (Apr 1997). "Differential ... there are also cases where NADK activity appears to potentiate cell death. Genetic studies done in human haploid cell lines ... "Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death". ACS Chemical Biology. 10 (7 ...

https://en.wikipedia.org/wiki/NAD _kinase

That night, to get Jason away from the Dragonfly Inn, Sookie and Michel call his cell pretending to be somebody else and tell ... Henry Cho (Eddie Shin) (2001-2002), a Chilton student who dated Lane. Bradley "Brad" Langford (Adam Wylie) (2001-2003), a ...

https://en.wikipedia.org/wiki/List_of_Gilmore_Girls_characters

Mol Cell. 2017;65(3):416-431.e6. Alessi DR, James SR, Downes CP, Holmes AB, Gaffney P, Reese CB, et al. Characterization of a 3 ... Dimitrios Karathanassis; Robert V. Stahelin; Jerónimo Bravo; Olga Perisic; Christine M Pacold; Wonhwa Cho; Roger L Williams ( ... Mol Cell. 2017;65(3):416-431.e6. Ebner M, Lučić I, Leonard TA, Yudushkin I. PI(3,4,5)P3 Engagement Restricts Akt Activity to ... Mol Cell. 2017;65(3):416-431.e6. Ebner M, Lučić I, Leonard TA, Yudushkin I. PI(3,4,5)P3 Engagement Restricts Akt Activity to ...

https://en.wikipedia.org/wiki/Phosphatidylinositol_3,4-bisphosphate

Plant Cell, Tissue and Organ Culture 68 (3): 241-246. Kimura, M. 2008. Effects of blanching treatment on the essential oil and ... Kim, C. J., Park, T. W., Cho, B. J., Lee, J. Y., Kim, T. D. 2010. The analgesic and anti-inflammatory effects of 7- ... Kim, C. J., Park, T. W., Cho, B. J., Lee, J. Y., Kim, T. D. 2010. Learn2grow. 2006-2013. Walters Gardens Inc. (2013). Weimer, J ...

https://en.wikipedia.org/wiki/Aralia_cordata

"Antagonism of the five cloned human muscarinic cholinergic receptors expressed in CHO-K1 cells by antidepressants and ...

https://en.wikipedia.org/wiki/Doxepin

MicroRNA Lee K, Kim JH, Kwon OB, An K, Ryu J, Cho K, Suh YH, Kim HS (April 2012). "An activity-regulated microRNA, miR-188, ... Mishra PK, Tyagi N, Kundu S, Tyagi SC (2009). "MicroRNAs are involved in homocysteine-induced cardiac remodeling". Cell ... "MicroRNA expression in human airway smooth muscle cells: role of miR-25 in regulation of airway smooth muscle phenotype". ... American Journal of Respiratory Cell and Molecular Biology. 42 (4): 506-13. doi:10.1165/rcmb.2009-0123OC. PMC 2848741. PMID ...

https://en.wikipedia.org/wiki/Mir-188_microRNA_precursor_family

Eun Ju Chang, Won Jung Lee, Sung Hee Cho and Sang Won Choi, Archives of Pharmacal Research, August 2003, Volume 26, Issue 8, ... Proliferative effects of flavan-3-ols and propelargonidins from rhizomes of Drynaria fortunei on MCF-7 and osteoblastic cells. ...

https://en.wikipedia.org/wiki/Propelargonidin

Ha BG, Hong JM, Park JY, Ha MH, Kim TH, Cho JY, Ryoo HM, Choi JY, Shin HI, Chun SY, Kim SY, Park EK (July 2008). "Proteomic ... transduce environmental signals into cell responses, or function in drug efflux. GRCh38: Ensembl release 89: ENSG00000164038 - ...

https://en.wikipedia.org/wiki/SLC9B2

... in ATM-mutant cells, an inhibitor of DNA-PKcs causes high levels of apoptotic cell death. In ATM mutant cells, additional loss ... Lee SW, Cho KJ, Park JH, Kim SY, Nam SY, Lee BJ, Kim SB, Choi SH, Kim JH, Ahn SD, Shin SS, Choi EK, Yu E (2005). "Expressions ... When cancer cells are deficient in ATM the cells are "addicted" to DNA-PKcs, important in the alternative DNA repair pathway ... After ionizing radiation DNA-PKcs was increased in the surviving cells of oral squamous cell carcinoma tissues. The ATM protein ...

https://en.wikipedia.org/wiki/DNA-PKcs

M cells) that allow for the uptake of antigens produced by pathogens. These M cells then alert the B cells and T cells in the ... Wang, JH; Chung, YS; Cho, YW; Kim, DY; Yi, JS; Bae, JS; Shim, MJ (April 2010). "Palatine tonsil size in obese, overweight, and ... The tonsils have on their surface specialized antigen capture cells called Microfold cell ( ... B cells are activated and proliferate in areas called germinal centers in the tonsil. These germinal centers are places where B ...

https://en.wikipedia.org/wiki/Tonsil

... is a protein that slows cell division by slowing the progression of the cell cycle from the G1 phase to the S phase, thereby ... Liu Y, Sanoff HK, Cho H, Burd CE, Torrice C, Ibrahim JG, Thomas NE, Sharpless NE (August 2009). "Expression of p16(INK4a) in ... also stem and progenitor cells of the aged dentate gyrus. In fact, after deletion of p16INK4a, stem cells of the dentate gyrus ... Cancer cells show a significant increase in the accumulation of methylation in CpG islands in the promoter region of p16. This ...

https://en.wikipedia.org/wiki/P16

Cho WC, Nam OH, Kim MS, Lee HS, Choi SC (May 2018). "A retrospective study of traumatic dental injuries in primary dentition: ... as this will damage the delicate cells that make up the tooth's interior. Seek emergency dental treatment immediately. When the ...

https://en.wikipedia.org/wiki/Dental_trauma

... prostate cancer cell population harbors self-renewing long-term tumor-propagating cells that resist castration". Cell Stem Cell ... ISBN 978-0-323-47674-4. Di Blasio CJ, Rhee AC, Cho D, Scardino PT, Kattan MW (October 2003). "Predicting clinical end points: ... LNCaP cells express AR, but PC-3 and DU-145 cells express very little or no AR. The proliferation of LNCaP cells is androgen- ... Prostate cancer cells are generally devoid of zinc. Prostate cancer cells save energy by not making citrate, and use the ...

https://en.wikipedia.org/wiki/Prostate_cancer

Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional ... Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells Cloning hybridomas and CHO cells can be done faster and more ... Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium Publish Date: July 17, 2013 ... Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning. During semi-solid cloning, ...

https://www.stemcell.com/technical-resources/educational-materials/videos-and-webinars/semi-solid-cloning-with-clonacell.html

Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues ... Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues ... Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues ... Sound affects the neuronal maturation of neuroblastoma cells and the repair of damaged tissues. Hyunjin Cho, Hee-Jung Park, Ju- ...

http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2258

Given that many production facilities were built to handle viable cell concentrations in the order of 106 cells/mL, cell ... While the addition of FeCl3, MnCl2 and CaCl2 did result in improved rates of sedimentation of CHO cells compared to untreated ... The objective of this thesis was to engineer a flocculating CHO cell line. Three distinct approaches are described. The first ... This thesis is an describes the first steps towards engineering a flocculating CHO cell line suitable for use with current ...

https://doras.dcu.ie/28007/

Transfection and selection data of CHO-K1 cells by various antibiotics in differing media. ... Cell-Lines , Plasmids , Privacy Policy , Terms of Use , About Us , Contact Us. Cell-Culture Database Version 1.1 ... Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like ...

https://cell-lines.toku-e.com/Cell-Lines_Detail_608.html

Improve Research Reproducibility A Bio-protocol resource ...

https://www.bio-protocol.org/exchange/preprintdetail?type=3&id=1429

A Chinese hamster ovary cell line that expresses a secreted form of the gp160 gene of an HIV isolated designated MW959. The ... The cell line secretes the protein in a constitutive manner into the culture supernatant.. Details for this material may be ... found in the NIH AIDS Reagent Program Catalog (Catalog# 4698): https://www.aidsreagent.org/reagentdetail.cfm?t=cell_lines&id=97 ...

http://www.techtransfer.nih.gov/tech/tab-3285

Mammalian cell culture-based manufacturing for biopharmaceuticals is perceived as an established technology. Many open ... Chromosomal and genomic heterogeneity of CHO cells, other cell lines, and cancer cells. Analyzing CHO cell lines in the 1960s ... A short review on the early history of CHO Cells. CHO cells have a culture history of more than 50 years, since they were ... Identical genetics among the cells of a cell population derived from a single cell (a CHO clone) is not supported by any data. ...

https://excellgene.com/2019/02/cloning-of-cho-cells-productivity-genetic-stability/

Recent advances in mammalian cell line development and discussion of the criteria a biotech company should consider when ... this platform remains based on a CHO cell line derived from CHO-K1 line, created by knocking out the glutamine synthetase (GS) ... If you currently are in the process development stage of your biotherapeutic candidate, looking for a CHO cell line development ... Nowadays, the expected standard for any new antibody development project is to obtain a CHO cell line with a productivity above ...

https://www.gtp-bioways.com/protein-engineering/which-platform-partner-for-cho-cell-line-development/

... as the protease responsible for the proteolysis of gp120 in CHO cells. CRISPR/Cas9 knockout of the C1s protease in a CHO cell ... C1s-deficient CHO cell line to express other recombinant proteins that are susceptible to serine protease activity in CHO cells ... cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV ... Similarly, the workflow described here to identify and knockout C1s in a CHO cell line can be applied to remedy the proteolysis ...

https://pesquisa.bvsalud.org/perinatal/resource/es/mdl-31087560

Simplified Small-Scale Harvest of CHO Cells for mAb Analytics. /* custom css theme panel */ #wp-admin-bar-hi { display: none ...

https://www.genengnews.com/sponsored/how-to-win-in-emerging-markets/

C3H/10T1/2 mouse cells. Abernethy et al. [1982]. * SCE: Sister chromatid exchange. [ return to table]. **CHO: Chinese hamster ... DNA-damaging activity of biotic and xenobiotic aldehydes in chinese hamster ovary cells. Cell Biochem Function 2:243-248. ... Human bronchial epithelial cells. Saladino et al. [1985]. Inhibited DNA synthesis. Reuber rat hepatoma (H35)and (Hep 10C). ... CHO = Chinese hamster ovary. [return to table]. †††HGPRT = Hypoxanthine guanine phosphoribosyl transferase. [return to table]. ...

https://www.cdc.gov/niosh/docs/91-112/default.html

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup ... The proposed method is applied to cultured human embryonic kidney cells, Chinese hamster ovary cells, human red blood cells, ... In addition to the absolute cell volume, the method can be applied to derive important biophysical parameters of living cells ... CHO Cells * Cell Membrane Permeability * Cell Size * Cells, Cultured * Cricetinae * Cricetulus * Glutamic Acid / ...

https://pubmed.ncbi.nlm.nih.gov/23487181/?dopt=Abstract

CARBACHOL STIMULATION CAUSES INHIBITION OF MITOGENESIS AND CELL ELONGATION IN CHO CELLS TRANSFECTED WITH MUSCARINIC RECEPTOR ... CARBACHOL STIMULATION CAUSES INHIBITION OF MITOGENESIS AND CELL ELONGATION IN CHO CELLS TRANSFECTED WITH MUSCARINIC RECEPTOR ...

https://www.neuroscience.ox.ac.uk/publications/906210

High-Throughput Lipidomic and Transcriptomic Analysis to Compare SP2/0, CHO, and HEK-293 Mammalian Cell Lines. ... Dive into the research topics of High-Throughput Lipidomic and Transcriptomic Analysis to Compare SP2/0, CHO, and HEK-293 ...

https://scholars.uthscsa.edu/en/publications/high-throughput-lipidomic-and-transcriptomic-analysis-to-compare-/fingerprints/

... from nontransfected CHO cells (NT) or from CHO cells transfected with Kv7.3, Kv7.3 A315T, or Kv7.3 R230C plasmids. In each ... from untransfected CHO cells (NT) or from CHO cells expressing Kv7.2 (WT), Kv7.2 D172R/R201D (DR/RD), or D172C/R201C (DR/CC) ... CHO) cells from female animals by transient transfection. CHO cells were grown in 100 mm plastic Petri dishes in DMEM ... Whole-cell electrophysiology.. Currents from CHO cells were recorded at room temperature (20°C-22°C) 1-2 d after transfection, ...

https://www.jneurosci.org/content/35/9/3782

CHO cells (2) E. coli (97) Goat (55) Hamster (Armenian) (11) Hamster (syrian) (1) Horse (5) Human (8) Human 293 Cells (19) ... Cell Extract Kit (22) Cell Extracts (17) Cellular Dyes (30) Chemical Modulators (221) ChIP Kit (12) CUT&RUN Kit (3) Detection ... In-cell western (4) IP (3457) Neutralizing Ab (2) Peptide ELISA (38) RNA Dot Blot (2) Simple Western™ (106) Transfection (144) ...

https://www.cellsignal.com/browse/

CHO Cells. CIDA use Canadian International Development Agency. CIDP use Polyradiculoneuropathy, Chronic Inflammatory ...

https://decs.bvs.br/I/DeCS2008_Alfab-C_ing.htm

Additional strain / cell type characteristics:. other: subclone CHO-KrBH4 of the CHO-K-, cell line, referred to as CHO cells. ... other: Chinese Hamster Ovary (CHO) cells Target gene:. hprt locus. Species / strain / cell type:. Chinese hamster Ovary (CHO). ... CHO cells were treated with DMA in the presence of metabolic activation (Aroclor-1254 induced rat liver microsomes) and ... Chinese Hamster Ovary (CHO) cells were used with and without metabolic activation system. The test concentration of ...

https://www.echa.europa.eu/web/guest/registration-dossier/-/registered-dossier/14794/7/7/1

Species / strain / cell type:. other: Chinese Hamster ovary (CHO) cells. Details on mammalian cell type (if applicable):. - ... mammalian cell gene mutation assay. Species / strain / cell type:. mouse lymphoma L5178Y cells. Metabolic activation:. with and ... Tissues and cell types examined:. bone marrow, alveolar macrophages, regenerating liver cells. 20 metaphases from 3 dishes were ... p-Cresol did not induce significant increases in SCE frequencies in any of the cell types examined: bone marrow cells, alveolar ...

https://echa.europa.eu/lt/registration-dossier/-/registered-dossier/15980/7/7/1

using SCRHs, mock-transfected CHO cells, or HEK293 cells. values estimated from these cell lines were higher than sinusoidal ... CHO cells. Chinese hamster ovary cells. CI. confidence interval. CL. clearance. biliary clearance. de-lactonization clearance. ... and CHO-mock cells (passive), the recreated in silico values from Oatp-expressing cells (active) and HEK293-mock cells (passive ... CHO-mock cells, and HEK293-mock cells. Data shown are the mean ± S.D. of five lots (SCRHs), three independent experiments (CHO- ...

https://dmd.aspetjournals.org/content/46/1/66?ijkey=aa9eadc1c063e21e63b5e3c3bf7b98a4c0537433&keytype2=tf_ipsecsha

Evaluated by Western Blot in Insulin treated CHO IR/IRS-2 cell lysate.. Western Blot Analysis: 0.5 µg/mL of this antibody ... detected IRS-2 on 10 µg of Insulin treated CHO IR/IRS-2 cell lysate. ... have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell ...

https://www.sigmaaldrich.com/US/en/product/mm/mabs15

RECOTHROM thrombin is produced in a genetically modified Chinese Hamster Ovary (CHO) cell line and may contain hamster or snake ... The cell line used to manufacture RECOTHROM has been tested and shown to be free of known infectious agents. The cell culture ... recombinant DNA technology from a genetically modified CHO cell line. RECOTHROM is identical in amino acid sequence and ... one patient also developed anti-CHO host cell protein antibodies); 43 of 200 patients in the bovine thrombin group (22%; 95% CI ...

https://dailymed.nlm.nih.gov/dailymed/lookup.cfm?setid=54885644-e51e-4263-aadb-366abaeb56a3&version=11

CHO (mammalian cells) (2) E. coli (1) NSO (mammalian cells) (3) Sf 21 (baculovirus)-derived (1) ...

https://www.anaspec.com/en/catalog/c87~assay-kits?catalog_product.ProductAttribute.222=21014

CHO Cells. Purified From HEK 293 Cell culture Supernatant.. Goat. chicken. Sheep. Donkey. Guinea Pig. Horse. Hamster. Mouse- ... T-cell expansion. Antigen specific T-cell stimulation. Immune monitoring. T-cell assays. IHC-Leica. IHC-P-Leica. FC-Leica. IF- ... These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion. ... Abceptas portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our ...

https://abgent.com/products/category/Secondary-Antibodies

SOURCE SPECIES: CHO cells. *IMMUNOGEN: Humanized / MET/RCCP2 [Homo sapiens]. *CONJUGATE: Unconjugated ...

https://www.prosci-inc.com/product/onartuzumab-met-rccp2-research-grade-biosimilar-10-454/

... followed by transportation of antigen to antigen-presenting cells (APCs) and stimulation of B and T cells [48]. Nevertheless, ... Islam, M.A.; Firdous, J.; Badruddoza AZ, M.; Reesor, E.; Azad, M.; Hasan, A.; Lim, M.; Cao, W.; Guillemette, S.; Cho, C.S. ... Preparation of the Inactivated Cells. The S. iniae was streaked onto TSA and incubated at 30 °C for 24-48 h and resulting S. ... The inactivated cells were resuspended in sterile PBS and using the McFarland standard. Approximately 1 mL of the vaccine was ...

https://www.mdpi.com/2076-393X/9/1/51

Intracellular Flow Cytometry analysis of CHO-PD-L1 (red) and CHO-S (blue) cells labelling PD-L1 with ab213524 at 1/500. Cells ... Lane 3 : U-87 MG cell lysate. Lane 4 : MCF7 cell lysate. Lane 5 : Wild-type A549 untreated cell lysate. Lane 6 : CD274 knockout ... Lane 3 : U-87 MG cell lysate. Lane 4 : MCF7 cell lysate. Lane 5 : Wild-type A549 untreated cell lysate. Lysates/proteins at 20 ... ICC/IF: CHO-PDL1 and NCI-H1975 cells. IP: NCI-H1975 whole cell lysate. ...

https://www.abcam.com/products/primary-antibodies/pd-l1-antibody-epr19759-bsa-and-azide-free-ab221612.html

While many perceive mammalian cell culture-based manufacturing for biopharmaceuticals an established technology, numerous open questions remain to be solved. (excellgene.com)

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. (abgent.com)
Presently, we have investigated the expression profile of TEF1A2 using a panel of human cancer cell lines and tumor tissues. (cdc.gov)
Up to a 2000-fold overexpression of TEF1A2 was detected in 90% (nine out of 10) of the cancer cell lines derived from various human tissues compared to their corresponding controls. (cdc.gov)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Genetic diversity and mutation rates in CHO cells have been underestimated since progeny of a clonal CHO cell become genetically diverse with each cell division. (excellgene.com)
Recombinant FSH production occurs in genetically modified Chinese Hamster Ovary (CHO) cells cultured in bioreactors. (rxlist.com)
CHO cells were cotransfected with the genetically encoded ﬂuorescent redox sensor Glutaredoxin-(Grx-) roGFP3 and COX1 using FUGENE-HD (Promega). (bmglabtech.com)

Browse our catalog to find the right whole cell lysate for your experiment. (abgent.com)
NCI-H1975 whole cell lysate. (abcam.com)
IP: NCI-H1975 whole cell lysate. (abcam.com)

Studies showing that murine fibroblast cell lines are susceptible to infection with mouse-adapted scrapie agent ( 11 , 12 ) increased concern that nonneuronal cell substrates used to propagate viruses for vaccine production might become infected with a TSE agent contaminating some component of culture medium, especially bovine serum ( 13 ). (cdc.gov)
Two diphtheria and tetanus toxoids and acellular pertussis (DTaP) vaccines -- ACEL-IMUNE{Registered} * and Tripedia{Registered} ** -- have been licensed for several years, but (until recently) only for administration of the fourth and fifth doses in the series to children aged 15 months-6 years who previously had received three or more doses of diphtheria and tetanus toxoids and whole-cell pertussis (DTP) vaccine. (cdc.gov)
Based on controlled efficacy trials conducted in the 1940s and on subsequent observational efficacy studies, a primary series comprising four doses of whole-cell DTP vaccine is considered 70%-90% effective in preventing serious pertussis disease (1-4). (cdc.gov)
More severe systemic events (e.g., convulsions {with or without fever} and hypotonic hyporesponsive episodes) occur less frequently (ratio of one case to 1,750 doses administered) among children who receive whole-cell DTP vaccine (5). (cdc.gov)
Experts disagree on whether whole-cell pertussis vaccine causes lasting brain damage, but agree that if the vaccine causes such damage it does so only rarely (7). (cdc.gov)

Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines . (bvsalud.org)
The proposed method is applied to cultured human embryonic kidney cells, Chinese hamster ovary cells, human red blood cells, mouse cortical astrocytes, and neurons. (nih.gov)
The objective of this in vitro assay was to evaluate the ability of p-cresol to induce chromosomal aberrations in chnese hamster ovary (CHO) cells with and without metabolic activation. (europa.eu)
p-Cresol induced chromosome aberrations in a test according to OECD TG 473 with Chinese Hamster Ovary cells in the presence and in the absence of a metabolic activation system and tested up to cytotoxicity. (europa.eu)
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). (bvsalud.org)

Using a realistic model of a feedforward inhibitory microcircuit in the hippocampal CA1 region, an increased excitability of pyramidal neurons was found upon incorporation of the experimentally defined parameters for mutant M-current, suggesting that changes in network interactions rather than in intrinsic cell properties may be responsible for the neuronal hyperexcitability by these gain-of-function mutations. (jneurosci.org)

Resulting from dialogs between the industry and regulatory agencies steps and procedures had been established, since the start of using CHO cells for pharmaceutical production, to reduce any perceived or real risks related to emergence of mutations or other genetic changes in protein generating cell populations. (excellgene.com)
When expressed in CHO cells , the recombinant HIV envelope protein , gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by neutralizing antibodies . (bvsalud.org)
We determined whether in vivo transporter-mediated hepatobiliary clearance (CL) and hepatic concentrations of rosuvastatin (RSV) in the rat could be predicted by transport activity in sandwich-cultured rat hepatocytes (SCRHs) and/or transporter-expressing cell lines scaled by differences in transporter protein expression between SCRHs, cell lines, and rat liver. (aspetjournals.org)
Protein expression of Oatps in SCRHs and Oatp-expressing cells was quantified by liquid chromatography tandem mass spectrometry. (aspetjournals.org)
into Oatp-expressing cells (active transport) plus passive diffusion CL in SCRHs, scaled by the difference in protein expression in Oatp cells versus SCRH versus rat liver, was within 2-fold of that observed in SCRHs or in vivo. (aspetjournals.org)
During the discovery phase of a process, one of the first considerations for biotherapeutic protein production is the choice of host cell. (bioprocessintl.com)

Microtubules (MTs) play a fundamental role in many vital processes such as cell division and neuronal activity. (frontiersin.org)
Many investigators once believed that TSE agents infected mainly, if not exclusively, cells of neuronal and lymphoid lineages. (cdc.gov)

Given that many production facilities were built to handle viable cell concentrations in the order of 106 cells/mL, cell concentrations of this magnitude have the potential to cause problems during primary recovery activities as existing processes/equipment may not be able to efficiently remove cells from these high cell concentration processes. (dcu.ie)

Histologically, NSCLC is divided into adenocarcinoma, squamous cell carcinoma (SCC) (see the image below), and large cell carcinoma. (medscape.com)
A cavitating right lower lobe squamous cell carcinoma. (medscape.com)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)

The first approach was the cloning, in CHO cells of four eukaryotic proteins known to promote flocculation. (dcu.ie)
Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. (bvsalud.org)

Most likely this is understood in a sense that the singularity of a cell and the derivation of dividing daughter cells from such a single cell would provide a (more) robust genetic foundation for reliable manufacture of recombinant proteins using cells. (excellgene.com)
Identification and CRISPR/Cas9 Inactivation of the C1s Protease Responsible for Proteolysis of Recombinant Proteins Produced in CHO Cells. (bvsalud.org)
Furthermore, the proteolysis issues caused by the C1s protease suggests a broader need for a C1s-deficient CHO cell line to express other recombinant proteins that are susceptible to serine protease activity in CHO cells . (bvsalud.org)

Therefore, we developed and validated a cell-based mode-of-action (MOA) assay, by which the total NSAID activity in a wastewater sample enriched by solid phase extraction (SPE) can be measured as equivalents of the lead substance diclofenac (DicEQ). (bmglabtech.com)
The Grx-roGFP3-COX1 sensor cell line was used to assay for NSAID activities in wastewater samples. (bmglabtech.com)
The MOA-based NSAID assay in living cells allows for measuring total NSAID activities in complex mixtures such as WWTP efﬂuents as equivalents of the lead substance diclofenac (DicEQ). (bmglabtech.com)
Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. (bvsalud.org)

Our focus is on targets covering the major research areas impacting health and disease, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. (abgent.com)

This is an important issue since products are made in bioreactors containing up to 10 14 cells that have divided for weeks. (excellgene.com)

CRISPR /Cas9 knockout of the C1s protease in a CHO cell line was shown to eliminate the proteolytic activity against the recombinantly expressed gp120. (bvsalud.org)
Similarly, the workflow described here to identify and knockout C1s in a CHO cell line can be applied to remedy the proteolysis of biologics by other CHO proteases . (bvsalud.org)

Over the last two decades, viable cell concentrations in industrial processes have reached multiples of 107 cells/mL at harvest. (dcu.ie)
This thesis is an describes the first steps towards engineering a flocculating CHO cell line suitable for use with current commercial processes. (dcu.ie)
We revisit decades of research on scope and rate of genetic changes in CHO cells and suggest approaches to minimize trends for genomic instability when establishing reliable manufacturing processes. (excellgene.com)
While the main focus of this article will be a summary and discussion of facts and mechanisms behind rapid evolution and mutational change of CHO populations, some of the critical steps for the establishment of reliable and stable CHO processes will be discussed in the latter part of these lines. (excellgene.com)

In previous studies, it was confirmed that sound stimulation promotes the neurodifferentiation process of mesenchymal stem cells. (ejbiotechnology.info)
In the in vivo study, mesenchymal stem cells were injected into the injured area, and then rats were exposed to sound for 4 weeks. (ejbiotechnology.info)
The hDPSCs expressed mesenchymal stem cell markers and served as an abundant source of stem cells with a high proliferation rate. (nature.com)

The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. (cdc.gov)

The nature of the proteins investigated in this thesis lead to challenges in achieving ectopic expression in CHO cells. (dcu.ie)
While the visual inspection seemed to imply that cells of an attached colony were in fact derived from a single cell, this did not constitute a proof of clonality and other more relevant tests were executed later in the process of cell line and manufacturing process development to assure the quality and consistency of proteins derived from such recombinant cells. (excellgene.com)
The association of cells with laminin 332 occurs through α3β1 integrin in focal adhesions and α6β4 in stable anchoring contacts, which contain an assembly of hemidesmosome proteins. (reprocell.com)
Translation factors are essential for cells to synthesize proteins, and their aberrant expression may result in pathological conditions including cancer. (cdc.gov)

Concerns about the safety of whole-cell pertussis vaccines prompted development of acellular vaccines that are less likely to provoke adverse events because they contain purified antigenic components of Bordetella pertussis. (cdc.gov)
Published reports indicate that, when administered to infants aged 2, 4, and 6 months, acellular pertussis vaccines are effective in preventing pertussis disease and associated with fewer local, systemic, and certain more serious adverse events than whole-cell pertussis vaccines. (cdc.gov)
Tripedia{Registered}, ACEL-IMUNE{Registered}, and Infanrix TM are now recommended for routine vaccination of infants and young children, although whole-cell pertussis vaccines remain acceptable alternatives. (cdc.gov)
Tripedia{Registered}, ACEL-IMUNE{Registered}, and Infanrix TM are recommended for all remaining doses in the schedule for children who have started the vaccination series with one, two, three, or four doses of whole-cell pertussis vaccines. (cdc.gov)
Four diphtheria and tetanus toxoids combined with whole-cell pertussis (DTP) vaccines are presently licensed for use in the United States. (cdc.gov)
Vaccines of this type, prepared from suspensions of inactivated Bordetella pertussis bacterial cells, have been licensed for routine vaccination of infants since the mid-1940s. (cdc.gov)

Transgenic CHO cells permanently overexpressing TEF1A2 were developed and used to investigate the role of TEF1A2 in apoptosis induced by cadmium - a well-known human chemical carcinogen. (cdc.gov)
Exposure of CHO cells to 2.5 and 10 um cadmium chloride for 24-hours resulted in significant apoptosis in all cell lines at 24- and 48-hours following the exposure. (cdc.gov)
However, the cadmium -induced apoptosis was significantly lower in the transgenic CHO cells permanently overexpressing TEF1A2 compared with the vector-alone transfected control cells. (cdc.gov)

The objective of this thesis was to engineer a flocculating CHO cell line. (dcu.ie)
Cell line and process stability are important for the manufacturing of pharmaceutical biologics. (excellgene.com)
The well-intended but incorrect regulatory suggestion is that cell line and process stability are linked to clonality. (excellgene.com)
At the time, cloning was done in the first authors' laboratory with cloning rings and cotton swabs from adherent cell cultures and many cells from a colony of cells were transferred into the next larger vessel for cell line establishment and testing. (excellgene.com)
In addition, the C1s-/- MGAT1- CHO cell line, with the C1s protease and the MGAT1 glycosyltransferase knocked out, enabled the production of unclipped gp120 from a clade B isolate (BaL-rgp120) and enriched for mannose -5 glycans on gp120 that are required for the binding of multiple broadly neutralizing monoclonal antibodies (bN-mAbs). (bvsalud.org)
No PrP TSE or infectivity has been detected in any exposed cell line under study so far. (cdc.gov)
They act high mortality rate associated with simple as first-line-of-defense cells in plasma and immune challenges in acquired immune de- perform phagocytosis either alone or in co- ficiency syndrome. (who.int)
The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells. (bvsalud.org)

may also play a role in the prevention of In recent years, the molecular biology of cancer by being able to detect abnormal cells. (who.int)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

The sheer diversity of genomic and phenotypic variation of CHO cell populations (and of other immortalized cell lines) is poorly understood and possibly underestimated. (excellgene.com)

iMatrix-332 is suitable for use as a substrate for culture of various cell types. (reprocell.com)
Success of the procedure is determined by use of a suitable scaffold, a structural support for osteoprogenitor cells and osteoinductive factors necessary to regenerate neo-bone at the site of bone defect 2 . (nature.com)

We are looking for an SR MES Automation Engineer I who wants to participate in our journey to establish the largest end-to-end cell culture CDMO facility in North America. (biospace.com)
This will be the largest end-to-end cell culture CDMO provider in North America. (biospace.com)

REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market. (reprocell.com)

Furthermore, selective heightened ac- work of Eric Newsholmes laboratory in the tivity of the immune system may explain a 1980s that it was established that immune number of pathological conditions (e.g., dia- cells such as lymphocytes and macrophages betes mellitus type I, multiple sclerosis, os- could utilize glutamine at high rates in addi- teoarthritis, rheumatoid arthritis, and ulcer- tion to glucose (3,4). (who.int)

While the addition of FeCl3, MnCl2 and CaCl2 did result in improved rates of sedimentation of CHO cells compared to untreated controls without any negative impact on a human antibodies structural integrity, the antibody yield at 62% for MnCl2,72% FeCl3 and 76% for CaCl2 was not commercially viable. (dcu.ie)
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.com)

The second approach used inorganic cations as flocculating agents and the third approach utilised proteomics to investigate cellular adhesion in CHO cells. (dcu.ie)
Additionally, the thesis proposes a potential mechanism which CHO cells developed to combat increased cellular stress during this adaptation process. (dcu.ie)
Elevation of intracellular cyclic AMP levels leads to diverse cellular responses dependent on the cell type. (nottingham.ac.uk)
Collectively, these results indicated that: 1) TEFA12 is significantly overexpressed in human tumor cell lines and tumor tissue samples, 2) the cellular expression level of TEF1A2 may be used as a tumor marker, and 3) the anti-apoptotic role of TEF1A2 may potentially be responsible for its oncogenic function. (cdc.gov)
The significance of the entiated end-cells whose ability to prolifer- cellular immune system in overcoming in- ate is gradually lost. (who.int)

In this work, we firstly used a computer-designed, solvent-free scaffold and human dental pulp stem cells (hDPSCs) to regenerate neo-bone within cranial bone defects. (nature.com)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
However, little is known about the exact anatomic location of these cells, and the relationship between stem cells and surrounding cells in dental pulp. (bvsalud.org)

These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion. (abgent.com)

Our study demonstrates that sound, with the advantage of being non-invasive and easy to use, is an effective stimulus that induces neural differentiation and maturation on animal cells. (ejbiotechnology.info)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

Despite the undoubted importance of the tine and liver, and tumor cells (5). (who.int)
The TNM (tumor-node-metastasis) staging system from the American Joint Committee for Cancer Staging and End Results Reporting is used for all lung carcinomas except small-cell lung cancer. (medscape.com)

Electrophysiological studies in mammalian cells transfected with human K v 7.2 and/or K v 7.3 cDNAs revealed that each of these four mutations stabilized the activated state of the channel, thereby producing gain-of-function effects, which are opposite to the loss-of-function effects produced by previously found mutations. (jneurosci.org)
iMatrix-332 is a highly purified and refined product of human recombinant laminin-332 (E8 fragment) expressed by CHO-S cells. (reprocell.com)
Laminin-332 is an important component of the "skin basement membrane" that exists between the epithelium and dermis of the skin, and is also expressed in human retinal pigment epithelium (RPE) and hair follicle cells. (reprocell.com)

Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Stem cell niche. (bvsalud.org)
that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)

That occurs within either the endoplasmic reticulum (ER) or the Golgi apparatus of a cell (GA) ( 3 - 5 ). (bioprocessintl.com)

A third area of research is the role of mitochondria in cell survival and/or toxicity following drug exposure or under substrate-limited conditions. (nottingham.ac.uk)

Sound is a kind of mechanical stimulus and has various effects on the growth and metabolism of plants and animal cells. (ejbiotechnology.info)
A recent cells of the immune system, it is surprising study from our laboratory has shown that that, until recently, relatively little was known neutrophils also utilize glutamine at high about their metabolism, the fuels they re- rates (6). (who.int)

Increases in the chromosomally aberrant cells ranging between 18 % and 40.5 % cells with aberrations(902 μg/ml was toxic, versus 1.5% of solvent control) and between 17 % and 43 % cells with aberrations (902 μg/ml was toxic, versus 3.0% of solvent control, respectively. (europa.eu)

In the in vitro study, SH-SY5Y cells were exposed to the sound for 3 days and then performed rt-PCR, FACS, and western blot for analysis. (ejbiotechnology.info)

Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)

Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancers. (medscape.com)
Non-small cell lung cancer. (medscape.com)
Go to Small Cell Lung Cancer for complete information on this topic. (medscape.com)
L'étude menée en Iraq a utilisé la méthode d'hybridation in situ pour déterminer la fréquence du papillomavirus humain et pour son génotypage dans les échantillons de tissus prélevés auprès de 129 patientes ayant reçu un diagnostic de cancer du sein malin, de 24 patientes porteuses d'une tumeur du sein bénigne et de 20 femmes témoins en bonne santé. (who.int)
99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)

Shimizu T. Tricultured cell sheets develop into functional pancreatic islet tissue with a vascular network. (reprocell.com)
It has become clear, however, that the susceptibility of cells to infection with TSE agents cannot be reliably predicted either from their tissue of origin or level of expression of PrP C ( 11 , 12 ). (cdc.gov)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

E6 and Metabolic syndrome and risks of colon and rectal renal cell carcinoma. (who.int)

All our cell lines and lysates are validated in key applications. (abgent.com)
Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). (cdc.gov)
All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent. (cdc.gov)

Giant cell arteritis is a systemic obliterative vasculitis mainly involving the arteries that originate from the arch of the aorta. (medscape.com)

In addition to the absolute cell volume, the method can be applied to derive important biophysical parameters of living cells including osmotic membrane water permeability coefficient and the integral intracellular refractive index (RI). (nih.gov)

Giant cell arteritis is a disease of elderly persons, the incidence of which increases with increasing age. (medscape.com)

Also, mechanisms resulting in mutational evolution of populations in cell culture in general have not been appreciated. (excellgene.com)

Anatomy4

Organisms2

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes13

Information Science3

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

Proteoglycan involvement in polyamine uptake. (4/15188)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

CHO Cells

Cricetinae

Cricetulus

Transfection

Ovary

Molecular Sequence Data

Cell Line

Amino Acid Sequence

Glycosylation

Cell Membrane

Cloning, Molecular

Base Sequence

Mutation

Recombinant Fusion Proteins

Kinetics

DNA, Complementary

Radioligand Assay

Endocytosis

Adenine Phosphoribosyltransferase

Cyclic AMP

Binding, Competitive

Receptors, Virus

Gene Expression

Protein Binding

Biological Transport

Choline

Diprenorphine

Cell Adhesion

DNA

Guanosine 5'-O-(3-Thiotriphosphate)

Tetrahydrofolate Dehydrogenase

Creatine

Protein Processing, Post-Translational

Polysaccharides

Membrane Glycoproteins

Membrane Proteins

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (1/15188)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (2/15188)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (3/15188)

Proteoglycan involvement in polyamine uptake. (4/15188)

Non-serum-dependent chemotactic factors produced by Candida albicans stimulate chemotaxis by binding to the formyl peptide receptor on neutrophils and to an unknown receptor on macrophages. (5/15188)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (6/15188)

Identification of a cytolethal distending toxin gene locus and features of a virulence-associated region in Actinobacillus actinomycetemcomitans. (7/15188)

Pseudomonas aeruginosa exoenzyme S is a biglutamic acid ADP-ribosyltransferase. (8/15188)

Protein tag

UB-612

Eftilagimod alpha

Etrolizumab

Pertuzumab

Pembrolizumab

COVID-19 vaccine clinical research

Enzyme replacement therapy

Zeocin

Motilin receptor

Leukotriene B4 receptor 1

Imaging cycler microscopy

Nivalenol

Deacetylasperulosidic acid

Transient expression

Blood vessel epicardial substance

Otenzepad

Muscarinic antagonist

2A self-cleaving peptides

Glycogen storage disease type II

KiSS1-derived peptide receptor

ZP3

Michael Stuart Brown

Excitatory amino acid transporter 1

SREBP cleavage-activating protein

Excitatory amino acid transporter 2

B4GALNT1

Nociceptin receptor

CLIC1

Fine chemical

New Delhi metallo-beta-lactamase 1

NOL3

Halobacterium salinarum

Signet ring cell carcinoma

HSPA1B

Viral synapse

$Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and...$ Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and...