Mercuriphenols substituted with one or more chlorine atoms and one or more nitro groups. Some of these are sulfhydryl reagents which act as chromophoric probes in enzymes and other proteins.

The homodimeric hemoglobin from Scapharca can be locked into new cooperative structures upon reaction of Cys92, located at the subunit interface, with organomercurials. (1/6)

In the cooperative, homodimeric hemoglobin from Scapharca inaequivalvis, HbI, the subunit interface is formed by the heme-carrying E and F helices and contains the only cysteine residue of the globin chain (Cys92, F2) in an area which changes from hydrophilic to hydrophobic upon oxygenation. Binding of organomercurials to HbI is cooperative and entails major quaternary rearrangements. The reaction of Cys92 with p-chloromercuri-benzoate (PMB) and p-nitro-o-chloromercuriphenol (PN), a sensitive reporter of the cysteine microenvironment at neutral pH values, has been followed in stopped flow experiments. Kinetic evidence for the cooperativity of mercurial binding has been obtained and the rate of the corresponding conformational transition has been estimated. As expected PN, but not PMB, is able to monitor the oxygen-linked change of the cysteine microenvironment. The modification of Cys92 with PN has unique functional effects. In PN-reacted HbI cooperativity is maintained, albeit to a different extent, depending on the ligation state of the protein during mercaptide formation. It may be envisaged that PN locks the protein into new, cooperative, quaternary structures stabilized by hydrogen bonding interactions between the ionized nitrophenol moiety and the contralateral subunit.  (+info)

Kinetic differentiation between enzyme inactivation involving complex-formation with the inactivator and that involving a conformation-change step. (2/6)

It has been suggested that the complexing type of inactivation in which the inactivator binds reversibly with the enzyme before inactivation cannot be differentiated kinetically from that a slow enzyme conformation change is involved as a first step [Rakitzis (1986) J. Theor. Biol. 122, 247-249]. The kinetics of the substrate reaction during modification of enzyme activity previously described [Tsou (1988) Adv. Enzymol. Relat. Areas Mol. Biol. 61, 381-436] have now been applied to this problem and equations derived to show that the slow-conformational-change type can be differentiated from the complexing type by plotting the final concentration of product formed, [P]infinity, against the reciprocal of inactivator concentration. The reaction of hexokinase with 2-chloromercuri-4-nitrophenol has been shown to involve a conformational change of the enzyme before inactivation.  (+info)

Use of isoelectric focusing and a chromophoric organomercurial to monitor urea-induced conformational changes of yeast phosphoglycerate kinase. (3/6)

The effects of urea in concentrations from 0 to 6M on the following properties of yeast phosphoglycerate kinase were studied: the kinetics of inactivation of the enzyme, the spectrum of 2-chloromercuri-4-nitrophenol bound to the single thiol group of the enzyme, the rate of reaction between the mercurial and enzyme, and the isoelectric point. The enzyme was inactivated by as much as 30% in 1M-urea, and the other data were interpreted as a possible 'tightening' of enzyme structure. The catalytic behaviour of the enzyme in 2M-urea was time-dependent, the initial effects being similar to those in 1M-urea. Polyacrylamide-gel isoelectric focusing of the enzyme in the presence of 2M-urea showed a single species of enzyme with an isoelectric point intermediate between those in 1M- and 3M-urea; a species with an identical isoelectric point was obtained after an 11-day exposure at 4 degrees C to the denaturant at 2M. The enzyme was rapidly inactivated in 3M-urea, with the thiol group fully exposed and the isoelectric point 0.9pH unit higher than in the absence of urea. No further conformational changes could be demonstrated with urea concentrations of 4M or greater. It is suggested that the equilibrium species that exists in 2M-urea has one of two buried lysine residues exposed. The second lysine residue is exposed in 3M or greater concentrations of the denaturant.  (+info)

A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group. (4/6)

2-(2'-Pyridylmercapto)mercuri-4-nitrophenol was synthesized and evaluated as a thiol-labelling reagent containing a chromophoric leaving group and as a reactivity probe by studies on its reactions with 2-mercaptoethanol and with papain (EC 3.4.22.2).  (+info)

Human 'creatine kinase conversion factor' identified as a carboxypeptidase. (5/6)

The effect of partially purified 'creatine kinase conversion factor' on rabbit muscle creatine kinase is shown to be that of a carboxypeptidase, removing the C-terminal lysine residue from both subunits. These changes fully explain the three-banded electrophoretic patterns of the partially and the fully modified rabbit and human enzymes. The factor also produces a similar electrophoretic pattern with haemoglobin A; comparison with the effects of carboxypeptidases A and B permits the inference that the C-terminal residues of both alpha- and beta-subunits are removed. Small synthetic peptides are poor or non-substrates. A low activity with hippuryl-L-lysine may be due to contamination of the preparation with carboxypeptidase N. The possibility has been excluded that the action of conversion factor on creatine kinase involves modification of the protein thiol groups. Mr, substrate-specificity, pH-activity profile and the effects of metal ions distinguish creatine kinase conversion factor from carboxypeptidases A, B and N. On the basis of this evidence it is proposed to give the conversion factor the provisional name of carboxypeptidase K.  (+info)

The essential sulfhydryl group of ornithine transcarbamylases. pH dependence of the spectra of its 2-mercuri-4-nitrophenol derivative. (6/6)

The essential sulfhydryl group of the ornithine transcarbamylases (ornithine carbamoyltransferase, 2.1.3.3) from bovine liver and Streptococcus faecalis reacts preferentially with 2-chloromercuri-4-nitrophenol. The spectra of this derivative between pH 4.4 AND 8.8 HAVE BEEN RESOLVED INto the spectrum of the nitrophenolate ion (III) and two species of phenol (I and II). The lambda max of I and II (both enzymes) and III (bovine) are red shifted from those of the comparable species in the same derivative of 2-mercaptoethanol. Deprotonation of a residue on the enzyme must be responsible for the transition from I to II. The pK values of the phenolic group are 7.1 (mercaptoethanol), 7.7 (bovine), and 8.8 (S. faecalis). The red shift in the lambda max of III and the modest increase in the pK of the phenolic group are consistent with a relatively hydrophobic environment for the nitrophenolate ion in the bovine enzyme. Since deprotonation of the residue in the bovine enzyme perturbs the pK of the phenolic group only slightly, its effect may be indirect. Interaction with a neighboring carboxyl group (pK 5.3) would account for the large increase in the pK of the phenolic group in the S. faecalis enzyme, which is not accompanied by an appreciable shift in the lambda max. Carbamyl-P increases the pK of the phenolic group in both enzymes, a result consistent with its binding site being close to the essential sulfhydryl group.  (+info)

Simon Cowells son Eric is looks set to make his first appearance on The X Factor later this year. MORE X FACTOR: Louis Walsh To Return To X Factor... But Not As A Judge Who Could Replace The ...
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ADDRESSING THE AGE FACTOR: SOME IMPLICATIONS FOR LANGUAGES POLICY Guide for the development of Language Education Policies in Europe From Linguistic Diversity to Plurilingual Education Reference Study
LeAnn Rimes & Carly Rose Sonenclar: X Factor Finale Performance!: Photo #2778491. LeAnn Rimes and Carly Rose Sonenclar sing their hearts out during the X Factor Season 2 Finale Performance Show on Wednesday (December 19) at CBS Television City…
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Exclusive: Paige Thomas talks about her time on The X Factor (Part 1). Paige Thomas talks about her The X Factor experience -- including whether she was surprised to get voted out at this point in the competition or saw it coming.
InTENsity: We felt we could beat Stereo Hogzz and survive on X Factor. InTENsity was eliminated from The X Factor, determining the new Fox reality competitions Top 11 acts, during the shows first live results show Thursday night.
LONDON -- One Direction, Katy Perry, The Killers and Elton John will appear on the finale of The X Factor in the U.K. One Direction will play the title track from their new album Midnight Memories, while Perry will perform Unconditionally.
Fear Factor lives again. MTV has revived the fan-favorite NBC reality show with a 12-episode reboot hosted and executive produced by Ludacris. But this time dont expect as many insect challenges.
MK1 have become the third act to be sent home from the X Factor after they failed to survive a deadlock against Gary Barlows act Kye Sones. Louis Walshs urban act MK1 performed The Scripts The M...
New York Mayor Bill de Blasio says the Trump factor had an effect on Tuesday elections that resulted in big wins for Democrats. Rough Cut (no reporter narration).
If the new trailer for Fear Factor is any indication, the show is bigger, badder and more extreme than ever!NBC just released the first video from reboot of the show, which went off the air after six seasons back in 2006, and its absolutely…
Simon Cowell didnt waste any time making his mark on The X Factor auditions at Nassau Coliseum Wednesday. Though the singing competitions lead judge was late for the afternoon taping, he immediat
Which finalist scored high marks for attitude? What was an American Idol alum doing on the X Factor stage? Is Simon Cowell friends with all his exes? THR has eyes and ears on the ground, backstage and beyond.
The X Factor bosses in the UK have a star in mind to fill the void left by Cheryl Cole as the Girls Aloud star appears to be taking her talents to the U.S. News of the World reports that Alesha Dixon of Mis-Teeq is being eyed for the role. The obvious advantage of Alesha is that shes young, sexy and confident and already has experience on a judging panel, a source said, referring to Dixons stint on Strictly Come Dancing.. The entire story at newsoftheworld.co.uk has since been removed.. ...
Jillian Jensen, a 19-year-old singer hailing from Massachusetts, performed an emotionally-charged rendition of Jessie Js Who You Are. Like Demi, Jillian had been a victim of bullying. Before her audition, she approached Demi saying, Im kind of a big fan of yours. I was also bullied a lot. For a long time I didnt know how to deal with it. I would cry myself to sleep every night. She then proceeded with an emotional audition, causing all four judges to get teary-eyed - even Simon Cowell!. ...
One fan said she was among those in the front queue but she did not get a good seat behind the judges because she was not attractive.
October extinguished itself in a rush of howling winds and driving rain and November arrived, cold as frozen iron, with hard frosts every morning and icy drafts that bit at exposed hands and faces. -J.K. Rowling
The singer is said to have agreed to make a guest appearance during the judges houses stage alongside her former boss Simon Cowell
In-depth critical reviews of Star Trek and some other sci-fi series. Includes all episodes of The Original Series, The Next Generation, Deep Space Nine, Voyager, Enterprise, Discovery, the new Battlestar Galactica, The Orville, and the Star Wars movies.
Were only three weeks in and already The X Factor is being whittled down to the Top 40 acts, 10 in each of the four categories. Im unaccustomed to reality competition shows moving this quickly, but given the disastrous ratings, who can blame them? ...
When I attended a taping of The X Factor in Seattle, I found myself cheering and clapping during several strong performances. But I was far more emotionally invested in watching those same performances on the small screen than I was able to muster in person.
Sharing articles I find, discussing options you might consider and suggesting what might help. Each person is different, what works for one might really mess up another. So what you do with this info is entirely up to you
Saara Aalto says the X Factor was the best but most exhausting experience. The Finnish singer, who got to the final of last years competition, thoroughly enjoyed taking part in the show because she was able to move to London but has admitted it was…
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A song called Here Come The Judges describes Jodys last album as a flop, and suggests Jordy is only on the show to revive her ailing career - just as Cheryl is rumoured to be returning to The X Factor. The musical has been approved by Simon, having been penned by his pal Harry Hill, and it is set to open in Londons West End next year. Cheryl was last humiliated by Simon after she was axed from the US version of The X Factor in 2011, but they finally settled their differences for good earlier this year, with the music mogul tweeting: We are close friends again and I adore her. ...
Read more about Britney Spears to bow out of X Factor? on Business Standard. Pop star Britney Spears might lose her 10 million pound per season deal with the X Factor USA, as her stint on the show has failed to increase the TRPs.
Papain (EC 3.4.22.2) is a proteolytic enzyme, the three-dimensional structure of which has been determined by x-ray diffraction at 2.8 A resolution (Drenth, J., Jansonius, J.N., Koekoek, R., Swen, H. M., and Wothers, B.G. (1968), Nature (London) 218, 929-932). The active site is a groove on the molecular surface in which the essential sulfhydryl group of cysteine-25 is situated next to the imidazole ring of histidine-159. The main object of this study was to determine by the difference-Fourier technique the binding mode for the substrate in the groove in order to explain the substrate specificity of the enzyme (P2 should have a hydrophobic side chain (Berger and Schechter, 1970) and to contribute to an elucidation of the catalytic mechanism. To this end, three chloromethyl ketone substrate analogues were reacted with the enzyme by covalent attachment to the sulfur atom of cysteine-25. The products crystallized isomorphously with the parent structure that is not the native, active enzyme but a ...
The top 16 on this season of The X Factor have been given the glam treatment just in time for the live shows to begin! Take a look at the before and after photos of the contestants.
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Demi Lovatos Metallic Dress on The X Factor - At this point, were starting to think Demi Lovatos closet is basically identical to Topshops store. The judge once again relied on the High Street retailer for an unforgettable TV look. For a futuristic style of your own, check out the brands Limited Edition Two-Tone Cut-Out Shift.See more looks from The X FactorOfficial site: The X Factor
If X Factor boss Simon Cowell were a schoolboy, hed have the class riveted with tales of his summer vacation. Theres the whole expecting a baby with his good friends estranged socialite wife thing. Theres this scary new poster for Foxs oft-stumbling little
Britney Spears has really been looking great for the X Factor auditions around the US -- and yesterday, she stepped out in a sexy little black dress ... and did it without hair extensions!The 30-year-old returned from her Hawaiian vacation with…
2-Amino-4-chloro-6-nitrophenol chemical properties, What are the chemical properties of 2-Amino-4-chloro-6-nitrophenol 6358-08-3, What are the physical properties of 2-Amino-4-chloro-6-nitrophenol ect.
Alfa Aesar™ 4-Methyl-2-nitrophenol, 97% 100g Alfa Aesar™ 4-Methyl-2-nitrophenol, 97% Mercaptoe to Methoxya -Organics
Balaji Telefilms creative director Ekta Kapoor speaks to Businessofcinema.com about LSD, the film which has sparked dialogue within the industry and more.
This tonic perfectly cleanses the skin, helping remove excessive sebum, impurities and make-up to maximize the effect of anti-aging skincare. Thanks to its more robust formulation, it leaves the skin more moisturized, toned and younger looking. The product is part of the Snake Factor series designed for the intensive correction of age-related skin changes. Products…
Simon Cowell has announced that Khloe Kardashian is in the running to host The X Factor USA. The reality star was first rumoured to join the judging panel
Aspen Skiing Co. Chief Operating Officer Mike Kaplan has one specific nightmare that plays out on a split screen in his minds eye.On one side of the screen is
Rajasthan Royals captain Rahul Dravid has brushed aside talks that temperamental pacer S Sreesanth was rested for the match against Mumbai Indians to avoid a possible confrontation with Harbhajan Singh, saying it was tactical move aimed at managing the workload of players.Ganesh AC has added a note - Dravid states the fact
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My adorable little leftist ass is far from a Romney supporter. But only two items on that list is something that makes me say I would not be comfortable voting for this man.. They are, of course, bullying the weak and not taking a firm stand on issues. But even then, its not like those are bad things because iThe lack of a firm stand makes it somewhat difficult to know what hed do in office, which is troubling. And bullying people who are weaker than you is fucking bullying people who are weaker than you, I am pretty sure every ethical system back to the days of primates is against that shit. That would be wrong even if it was the Manliest Man Thing In All Of Manlidom.. Incidentally, what is with the wealth paragraphs? Are our standards so low that, as Americans, were okay with having a hereditary aristocracy of wealth as long as they dont remind us of it too much?. I am deeply uncomfortable with the idea that people have to pass some kind of Manliness Test before they become president. ...
Mario Lopez has confessed that he is thrilled about landing a hosting job on The X FactorUSA. Earlier this week, the actor was named as one of the shows
is a potential judge, but remained vague. I cant confirm or deny anything right now, he said. The truth is, we are talking to a number of people. It changes on a daily basis to be honest with you ...
So, are you sick and tired of all the Oscars coverage yet? Well, heres something equally mind-numbing to perhaps distract you for a solid couple...
The X-Factor (FOX) - Season finale. Its Melanie Amaro v. Chris Rene v. Josh Krajcik (all terrible pop star names) to become Americas idol. Im voting for Josh because of his initials, and also because he didnt write a rap called
Simon Cowell and his sharp tongue are back! On The Tonight Show With Jay Leno, Cowell offered unfailingly honest opinions of his fellow judges on
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PolygamousRanchKid writes with this quote from an article in the Economist: The directors of Yahoo! were so spooked by being cast as the worst board in the country that they fired Carol Bartz as chief executive to show that theyre not the doofuses that they are. That was Ms Bartzs typically ...
PROJCS[unnamed, GEOGCS[unnamed ellipse, DATUM[unknown, SPHEROID[unnamed,6371000,0]], PRIMEM[Greenwich,0], UNIT[degree,0.0174532925199433]], PROJECTION[Mercator_1SP], PARAMETER[latitude_of_origin,0], PARAMETER[central_meridian,0], PARAMETER[scale_factor,1], PARAMETER[false_easting,1000], PARAMETER[false_northing,1000], UNIT[km,1]] ...
Jennifer Lopez has advised Britney Spears and Demi Lovato to be true to themselves when they begin their judging roles on The X Factor USA.The 42-year-old American Idol judge said that she felt scared for 30-year-old Britney and 19-year-old Demi...
s. 7 ep. 21 The Human Factor s. 7 ep. 22 Human Trials s. 5 ep. 11 Ripper s. 5 ep. 22 Better Luck Next Time s. 5 ep. 12 Tribunal s. 6 ep. ...
PROJCS[Lao_1997_UTM_48N, GEOGCS[GCS_Lao_1997, DATUM[Lao_National_Datum_1997, SPHEROID[Krasovsky_1940,6378245.0,298.3]], PRIMEM[Greenwich,0.0], UNIT[Degree,0.0174532925199433]], PROJECTION[Transverse_Mercator], PARAMETER[False_Easting,500000.0], PARAMETER[False_Northing,0.0], PARAMETER[Central_Meridian,105.0], PARAMETER[Scale_Factor,0.9996], PARAMETER[Latitude_Of_Origin,0.0], UNIT[Meter,1.0]] ...
Cheryl Cole has been confirmed as a judge on the US version of The X Factor.Simon Cowell has revealed the Promise This singer will join him and record executive L.A. Reid - as well as former American Idol judge Paula...
The Chosun Ilbo examines the Wie Factor: Her Korean-ness also plays a part. Despite her American nationality, when Wie answers questions asked in English in her not-so-fluent Korean, and when she introduces herself by her Korean name Sung-mi instead of Michelle, hearts here melt. Wie stressed that heritage during last weeks visit. In contrast to…

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