Chloride and mercury-containing derivatives of benzoic acid.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Research that involves the application of the natural sciences, especially biology and physiology, to medicine.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Organizations representing specialized fields which are accepted as authoritative; may be non-governmental, university or an independent research organization, e.g., National Academy of Sciences, Brookings Institution, etc.
An order of ascomycetous FUNGI which includes many economically important plant parasites as well as saprophytes.
A plant genus of the family MYOPORACEAE. Members have been used in FOLK MEDICINE.
An enzyme that catalyzes the endohydrolysis of 1,6-alpha-glucosidic linkages in isomaltose and dextrins produced from starch and glycogen by ALPHA-AMYLASES. EC 3.2.1.10.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
The ability to speak, read, or write several languages or many languages with some facility. Bilingualism is the most common form. (From Random House Unabridged Dictionary, 2d ed)
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
A verbal or nonverbal means of communicating ideas or feelings.
The sum or the stock of words used by a language, a group, or an individual. (From Webster, 3d ed)

A novel endonuclease of human cells specific for single-stranded DNA. (1/340)

We have fractionated from human aneuploid cell cultures three different enzyme fractions degrading single-stranded DNA. We have purified and characterized one of these DNases; this is an endonuclease working at alkaline pH (around 9.5) and requiring Mg2+ for its activity. The enzyme degrades denatured DNA over 100 times more efficiently than native DNA in optimal conditions. The termini produced by the enzyme have 5'P and 3'OH ends. The enzyme can attack, though at reduced rate, the supertwisted circular molecule of Simian virus 40 DNA, whereas it is inactive on the nicked circular molecule. The ultraviolet irradiation of DNA, whether native or denatured, does not affect its efficiency as substrate of the DNase. The properties of this endonuclease distinguish it from those of the other DNases described previously in mammalian cells; the denomination DNase VI is therefore proposed. Its properties are similar to those of DNases described in Ustilago maydis and Bacillus subtilis, for which an essential role in recombination seems likely.  (+info)

The aconitase of yeast. IV. Studies on iron and sulfur in yeast aconitase. (2/340)

Chemical analyses were carried out to determine the active components of the crystalline aconitase [EC 4.2.1.3] of Candida lipolytica. The enzyme contained 2 atoms of non-heme iron, 1 atom of labile sulfur, and 6 sulfhydryl groups per molecule. One atom of the non-heme iron was released by the addition of metal-chelating agents such as sodium citrate, sodium nitrilotriacetate (NTA) or sodium ethylenediaminetetraacetate (EDTA) without loss of the enzyme activity. The non-heme iron and labile sulfur were released by the addition of sulfhydryl reagents such as rho-chloromercuribenzoate (PCMB), sodium mersalyl or urea with loss of the enzyme activity. o-Phenanthroline reacted with the iron atoms in the enzyme at pH 6.0 with loss of the activity. These results show that yeast aconitase is an iron-sulfur protein and that only one of the two non-heme iron atoms is essential for enzyme activity.  (+info)

In vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in Streptococcus lactis. (3/340)

Two novel procedures have been used to regulate, in vivo, the formation of phosphoenolpyruvate (PEP) from glycolysis in Streptococcus lactis ML3. In the first procedure, glucose metabolism was specifically inhibited by p-chloromercuribenzoate. Autoradiographic and enzymatic analyses showed that the cells contained glucose 6-phosphate, fructose 6-phosphate, fructose-1,6-diphosphate, and triose phosphates. Dithiothreitol reversed the p-chloromercuribenzoate inhibition, and these intermediates were rapidly and quantitatively transformed into 3- and 2-phosphoglycerates plus PEP. The three intermediates were not further metabolized and constituted the intracellular PEP potential. The second procedure simply involved starvation of the organisms. The starved cells were devoid of glucose 6-phosphate, fructose 6-phosphate, fructose- 1,6-diphosphate, and triose phosphates but contained high levels of 3- and 2-phosphoglycerates and PEP (ca. 40 mM in total). The capacity to regulate PEP formation in vivo permitted the characterization of glucose and lactose phosphotransferase systems in physiologically intact cells. Evidence has been obtained for "feed forward" activation of pyruvate kinase in vivo by phosphorylated intermediates formed before the glyceraldehyde-3-phosphate dehydrogenase reaction in the glycolytic sequence. The data suggest that pyruvate kinase (an allosteric enzyme) plays a key role in the regulation of glycolysis and phosphotransferase system functions in S. lactis ML3.  (+info)

Membrane-bound DD-carboxypeptidase and transpeptidase activities from Bacillus megaterium KM at pH 7. General properties, substrate specificity and inhibition by beta-lactam antibiotics. (4/340)

1. The membranes from Bacillus megaterium KM contained a DD-carboxypeptidase with optimum activity under the following conditions: pH 7; ionic strength, 1.3 M; temperature, 40 degrees C and below 20 degrees C. It did not require any divalent cation, but was inactivated by Cu2+ and Hg2+. It was stimulated by 2-mercaptoethanol and low concentrations of p-chloromercuribenzoate. 2. The membrane preparation also catalyzed a simple transpeptidation reaction using as carboxyl acceptors D-alanine or glycine. 3. The conditions for optimum activity, temperature-inactivation, temperature-dependence of the activity, carboxyl donor specificity, sensitivity to beta-lactam antibiotics, and insensitivity to potential peptide inhibitors of both enzyme activities, was identical. The DD-carboxypeptidase showed inhibition by D-alanine and Ac2-L-Lys-D-Ala. 4. The inhibition by beta-lactam antibiotic was reversible for both enzymic activities and the time-dependence for their recovery was identical. 5. The DD-carboxypeptidase was very sensitive to changes in the configuration and size of the side-chains of the C-terminal dipeptide of the substrate. Amino acid residues at the C-terminus that precluded the peptide from being a DD-carboxypeptidase substrate were not acceptors in the transpeptidation reaction. Dipeptides were not acceptors for the 'model transpeptidase'. 6. It is suggested that both activities are catalysed by the same enzyme molecule, whose physiological role is not the formation of peptide crosslinks during peptidoglycan biosynthesis.  (+info)

Cardiac myosin from pig heart ventricle. Purification and enzymatic properties. (5/340)

A method is described for the preparation of high purity myosin from the left ventricle of pig heart. The purified myosin was free from nucleic acid, actin, tropomyosin, troponin, the 150,000 molecular weight protein and other contaminants. Analyses of subunits in the purified myosin were carried out on 3.5% acrylamide gel with 0.1% SDS. Of the total protein present in myosin, 11.3% was in the light chains; light chain 1 (LC1), 5.9% and light chain 2 (LC2), 5.4%. Urea gel electrophoresis of the purified myosin showed three closely spaced bands corresponding to the 20,000 dalton, the charge-modified 20,000 dalton and the phosphorylated 20,000 dalton components. The properties of the Ca2+-activated and K+-activated ATPases [EC 3.6.1.3] of the purified myosin were also studied. The Km values were 27 and 55 muM and the Vmax values were 0.263 and 0.317 mumole P1/mg/min for the Ca2+-activated and K+-activated ATPases, respectively. The pH-activity profiles and the effects of SH modification were of the skeletal myosin type except that the activities were lower.  (+info)

Pediococcus cerevisiae mutant with altered transport of folates. (6/340)

A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent process, and a concentrative uptake was also found in gluocse-starved cells and in cells incubated at OC. About 75% of the accumulated 5-CH3-H4PteGlu exchanged with amethopterin. In contrast to the wild type, the mutant accumulated both diastereoisomers of 5-CH3-H4PteGlue by glucose-dependent and glucose-independent processes. Amethopterin and PteGlue competitively inhibited the uptake in both processes, with an apparent lower affinity of the carrier for PteGlu than for the analogue. p-Chloromercuribenzoate strongly inhibited the uptake (75%). The p-chloromercuribenzoate-nonsusceptible and temperature-independent uptake was also competed by amethopterin. Metabolic poisons like sodium azide, potassium fluoride, iodoacetate, and 2,4-dimitrophenol inhibited the glucose-dependent process. Uptake, in the absence of glucose, was enhanced by sodium azide and potassium fluoride.  (+info)

The involvement of sulphydryl groups in the peptidyl transferase centre of eukaryotic ribosomes. (7/340)

Treatment of mammalian ribosomes with N-ethylmaleimide enhances up to 100% the ribosome efficiency in the "fragment reaction assay" for peptide bond formation by increasing the affinity of the substrate C-A-C-C-A-Leu-Ac for the donor site. This stimulation in peptidyl transferase activity was not observed when yeast ribosomes were treated in a similar manner. Stimulation of the peptidyl transferase activity of mammalian ribosomes was also observed by treatment with either p-chloromercuribenzoic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) or 5,5'-dithiobis-(2-nitropyridine) or the maleimide-derived antibiotic showdomycin. N-Ethylmaleimide treatment also enhances C-A-C-C-A-Leu binding to the acceptor site of the peptidyl transferase centre. However, neither binding of N-Ac-Phe-tRNA in the presence of ethanol, nor binding of Phe-tRNA to the ribosomes is stimulated by N-ethylmaleimide. The antibiotic tenuazionic acid (a selective inhibitor of peptide bond formation by mammalian ribosomes) appears to require for its inhibitory effect the ribosome sulphydryl residues, since its inhibitory action on the fragment reaction is greatly decreased in ribosomes treated with N-ethylmaleimide.  (+info)

Purification and properties of prostaglandin D synthetase from rat brain. (8/340)

The prostaglandin D synthetase system was isolated from rat brain. Prostaglandin endoperoxide synthetase solubilized from a microsomal fraction catalyzed the conversion of arachidonic acid to prostaglandin H2 in the presence of heme and tryptophan. Prostaglandin D synthetase (prostaglandin endoperoxidase-D isomerase) catalyzing the isomerization of prostaglandin H2 to prostaglandin D2 was found predominantly in a cytosol fraction and was purified to apparent homogeneity with a specific activity of 1.7 mumol/min/mg of protein at 24 degrees C. The enzyme also acted upon prostaglandin G2 and produced a compound presumed to be 15-hydroperoxy-prostaglandin D2. Glutathione was not required for the enzyme reaction, but the enzyme was stabilized by thiol compounds including glutathione. The enzyme was inhibited by p-chloromercuribenzoic acid in a reversible manner. The purified enzyme was essentially free of the glutathione S-transferase activity which was found in the cytosol of brain.  (+info)

Incubation of cultured bovine adrenal medullary cells with p-chloromercuribenzoate (50-500 microM), a sulfhydryl-reacting agent, caused an increase in the secretion of catecholamines, p-Chloromercuriphenyl sulfonate, a p-chloromercuribenzoate analogue that poorly penetrates the cell membrane, caused a similar increase in catecholamine secretion. In both cases, catecholamine secretion was dependent on extracellular Ca2+. Furthermore, p-chloromercuribenzoate caused both 45Ca2+ influx into the cells and an increase in the intracellular free Ca2+ concentration. The increases in catecholamine secretion and 45Ca2+ influx behaved similarly in relation to p-chloromercuribenzoate concentration. The time courses of the increased secretion, 45Ca2+ influx, and intracellular free Ca2+ concentration by p-chloromercuribenzoate were also quite similar. The stimulation of catecholamine secretion by p-chloromercuribenzoate was reversed by washing the cells with dithiothreitol-containing medium, but not by dithiothreitol
1GGV: Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF).
Yves, You dont say whether or not you are briefly fixing the sections. The binding of the b-galactosidase to lysosomes is relatively loose, and that may contribute to what you are seeing. Brief fixation in paraformaldehyde vapor may suffice. Also, something worth considering, have you tried inhibiting the enzyme with e.g. D-galactonolactone, lactose and/or p-chloromercuribenzoate? This is only speculation as I have never come across this phenomenon personally, but, as with Ray, have never performed the technique on pancreas.=0D One way to get by the problem of diffusion artefact, if that is what this is, would be to employ a semi-permeable membrane enzyme =0Ahistochemical technique. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Childrens Hospital =0A700 Childrens Drive Columbus, OH 43205 (614) 722 5465 [email protected] Columbus Childrens Hospital is now Nationwide Childrens Hospital www.NationwideChildrens.org=0D -----Original Message----- ...
Substrate effects on the activation kinetics of Chinese hamster dihydrofolate reductase by p-chloromercuribenzoate (pCMB) have been studied. On the basis of the kinetic equation of substrate reaction in the presence of pCMB, all modification kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present study indicate that the modification of Chinese hamster dihydrofolate reductase by pCMB shows single-phase kinetics, and that changes in the enzyme activity and tertiary structure proceed simultaneously during the modification process. Both substrates, NADPH and 7,8-dihydrofolate, protect dihydrofolate reductase against modification by pCMB. In the presence of a saturating concentration of NADPH, the value of kcat for 7,8-dihydrofolate in the enzyme-catalysed reaction increased four-fold on modification of Cys-6, accompanied by a two-fold increase in Km for the modified enzyme. The utilization of the binding energy of a ...
1. The ring-fission of cis- and trans-acenaphthene-1,2-diol by rat liver microsomes was studied. 2. 1,8-Naphthalic acid was detected and isolated after microsomal incubations of the diols. 3. The accompanying reduction of NAD+ was followed spectrophotometrically. 4. The optimum pH for the microsomal reaction was 9·4 for the oxidation of the cis-diol and 9·8 for that of the trans-diol. 5. p-Chloromercuribenzoate and 2,4-dichlorophenol inhibited the reaction. 6. Possible mechanisms for the microsomal ring-fission, involving 1,8-naphthalic aldehyde, are discussed.. ...
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TY - JOUR. T1 - Cobalt porphyrin catalyzed reduction of CO2. Radiation chemical, photochemical, and electrochemical studies. AU - Behar, D.. AU - Dhanasekaran, T.. AU - Neta, P.. AU - Hosten, C. M.. AU - Ejeh, D.. AU - Hambright, P.. AU - Fujita, Etsuko. PY - 1998/4/23. Y1 - 1998/4/23. N2 - Several cobalt porphyrins (CoP) have been reduced by radiation chemical, photochemical, and electrochemical methods, in aqueous and organic solvents. In aqueous solutions, the CoIP state is stable at high pH but is shorter lived in neutral and acidic solutions. Stable CoIP is also observed in organic solvents and is unreactive toward CO2. One-electron reduction of CoIP leads to formation of a species that is observed as a transient intermediate by pulse radiolysis in aqueous solutions and as a stable product following reduction by Na in tetrahydrofuran solutions. The spectrum of this species is not the characteristic spectrum of a metalloporphyrin π-radical anion and is ascribed to Co0P. This species binds ...
Scanaflo can find out if you are diabetic, pregnant or have been smoking weed, just with a single pee on the stick. It measures up to 12 reagents on the strip.
View Notes - General Properties of Viruses-review from MCB 2000 at University of Florida. General Properties of Viruses Structure 1. Nucleic acid - Single or double stranded. Segmented or
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
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USA - CP Soccer, an online store that has already won the trust of soccer enthusiasts of all ages, recently rolled out the much talked-about soccer boot - the Nike Mercurial Superfly V CR7. Along with the Nike Mercurial Superfly, the company has also launched Nike Hypervenom Blackout and Nike Mercurial Superfly Blue. The owners of the store said that they are now offering factory-direct prices on all of their products. Also, they indicated that returning customers can now redeem coupons and promo codes while buying the Mercurial Superfly and other recently rolled out products.. According to one of the sales executives of CP Soccer, the Mercurial Superfly V CR7 Chapter 5 cut-to-brilliance come in three color variants - black, white and blue tint.. Its an elegant shoe that every Christiano Ronaldo die-hard fan is possibly familiar with. The Mercurial design is the biggest selling proposition of this particular soccer cleat. Its regularly used by worlds most dangerous strikers, and the likes of ...
An intracellular β-glucosidase (EC 3.2.1.21) from Debaryomyces hansenii Y-44 was purified and characterized. The enzyme was purified to homogeneity from the cell-free extracts by a combination of column chromatography with DEAE-Toyopearl 650M and Butyl-Toyopearl 650S. The size of the enzyme was 95 kDa by native-PAGE, and the pl was 4.9. The enzyme was the most active at pH 7.0 and at around 25°C. The activity was highly tolerant to glucose, and only 20% inhibited in 500 mM glucose. The enzyme was tolerant to ethanol; its activity was only slightly reduced by 6% in the presence of 15% (v/v) ethanol. As the enzyme was inhibited with p-chloromercuribenzoate, it belongs to the class of SH-enzymes. The enzyme efficiently released monoterpenols from the glycosides extracted from Muscat grape must. The fermentation of Muscat juice coupled with the enzyme addition produced a considerable increase in the concentration of monoterpenols. Especially the linalool and nerol contents increased by 90% and ...
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Minken, S. L., DeWeese, J. A., Southgate, W. A., Mahoney, E. B. & Rob, C. G., May 1 1968, In : Surgery Gynecology and Obstetrics. 126, 5, p. 1056-1060 5 p.. Research output: Contribution to journal › Article ...
Chloromercuribenzoates/pharmacology. MESH. Dogs. MESH. Esophagus/metabolism. MESH. Guanidines/pharmacology. MESH. Guinea Pigs. ...
TY - JOUR. T1 - Proteolytic activity of a rumen anaerobic fungus. AU - Wallace, R. J.. AU - Joblin, K. N.. N1 - Spare prints in BOX no II.. PY - 1985/8. Y1 - 1985/8. N2 - A strain of the anaerobic phycomycetous fungus Neocallimastix frontalis isolated from the rumen of a sheep had a high proteolytic activity which became predominantly extracellular during growth. Proteolytic activity appeared to be due to a metalloprotease, as it was inhibited by 1,10-phenanthroline, EDTA and other chelators but not by phenylmethylsulphonyl fluoride (PMSF). Inhibition by EDTA was fully reversed by the addition of Zn2+, Ca2+ or Co2+, whereas addition of metal ions in the presence of 1,10-phenanthroline restored only a little activity. p-Chloromercuribenzoate (PCMB) was also inhibitory in dialysed supernatant fluid. N-α-p-Tosyl-l-lysine chloromethylketone (TLCK) inhibited proteolysis, suggesting that the protease(s) has a trypsin-like specificity, but benzoylarginine p-nitroanilide was not hydrolysed. Protease ...
chloromercuribenzoates plural of chloromercuribenzoate. → Definition and anagrams of chloromercuribenzoates. → Other senses and ...
Chloromercuribenzoates/pharmacology. *Hemoglobins/analysis*. *Humans. *Hydrogen-Ion Concentration. *Magnetic Resonance ...
Fingerprint Dive into the research topics of Overexpression of the ubiquitin-conjugating enzyme Cdc34 confers resistance to methylmercury in Saccharomyces cerevisiae. Together they form a unique fingerprint. ...
Chloromercuribenzoates Medicine & Life Sciences * Mercuric Chloride Medicine & Life Sciences 完全なフィンガープリントを表示 ...
Chloromercuribenzoates Medicine & Life Sciences * Cellobiose Medicine & Life Sciences * Ascomycota Medicine & Life Sciences ...
Biological Transport, Cell Membrane Permeability, Chlormerodrin, Chloromercuribenzoates, Erythrocytes, Glucose, metabolism, ...
Acetaldehyde, pharmacology, Alcohol Oxidoreductases, metabolism, Amides, Catalysis, Chloromercuribenzoates, Enzymes, Hydrogen- ...
D1.632.750.100.229 Chloromercuribenzoates D1.632.750.740.644.261 Chloromercurinitrophenols D1.632.750.740.225 Chloroplast ...
D2.241.223.200.185 Chloromercuribenzoates D2.241.223.100.140.100.500.261 D2.241.223.100.200.311 D2.241.223.100.500.261 D2.455. ...
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Chloromercuribenzoates * Dicyclohexylcarbodiimide * In Vitro Techniques * Mitochondria, Heart * Oligomycins * Oxidative ...
Animals , Chloromercuribenzoates/pharmacology , Ethylmaleimide/pharmacology , Fabaceae/enzymology , Glyceraldehyde-3-Phosphate ...
Chloromercuribenzoates - pharmacology , NAD - metabolism , Hydrogen-Ion Concentration , Index Medicus ...
Animals , Chloromercuribenzoates/pharmacology , Ethylmaleimide/pharmacology , Fabaceae/enzymology , Glyceraldehyde-3-Phosphate ... Chloromercuribenzoates/pharmacology , Dithionitrobenzoic Acid/pharmacology , Escherichia coli/ultrastructure , Ethylmaleimide/ ...
Aniline Compounds Borohydrides Chemical Phenomena Chemistry Chloromercuribenzoates Chromatography, Gel Chromatography, Ion ...