Chloroflexi: Phylum of green nonsulfur bacteria including the family Chloroflexaceae, among others.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Hydrocarbons, HalogenatedGeologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Actinobacteria: Class of BACTERIA with diverse morphological properties. Strains of Actinobacteria show greater than 80% 16S rDNA/rRNA sequence similarity among each other and also the presence of certain signature nucleotides. (Stackebrandt E. et al, Int. J. Syst. Bacteriol. (1997) 47:479-491)Sewage: Refuse liquid or waste matter carried off by sewers.Hydrothermal Vents: Hot springs on the ocean floor. They are commonly found near volcanically active places such as mid-oceanic ridges.Chloroflexus: A genus of green nonsulfur bacteria in the family Chloroflexaceae. They are photosynthetic, thermophilic, filamentous gliding bacteria found in hot springs.Proteobacteria: A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.Waste Management: Disposal, processing, controlling, recycling, and reusing the solid, liquid, and gaseous wastes of plants, animals, humans, and other organisms. It includes control within a closed ecological system to maintain a habitable environment.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Biodiversity: The variety of all native living organisms and their various forms and interrelationships.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Hot Springs: Habitat of hot water naturally heated by underlying geologic processes. Surface hot springs have been used for BALNEOLOGY. Underwater hot springs are called HYDROTHERMAL VENTS.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Microbial Consortia: A group of different species of microorganisms that act together as a community.Metagenome: A collective genome representative of the many organisms, primarily microorganisms, existing in a community.DNA, Archaeal: Deoxyribonucleic acid that makes up the genetic material of archaea.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Bacterial Load: Measurable quantity of bacteria in an object, organism, or organism compartment.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Tetrachloroethylene: A chlorinated hydrocarbon used as an industrial solvent and cooling liquid in electrical transformers. It is a potential carcinogen.Trichloroethylene: A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.Salicylanilides: 2-Hydroxy-N-phenylbenzamides. N-phenyl substituted salicylamides. Derivatives have been used as fungicides, anti-mildew agents and topical antifungal agents. In concentrated form may cause irritation of skin and mucous membranes.MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.Consumer Health Information: Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Bacteriology: The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.Waste Disposal, Fluid: The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.Waste Water: Contaminated water generated as a waste product of human activity.Industrial Waste: Worthless, damaged, defective, superfluous or effluent material from industrial operations.Access to Information: Individual's rights to obtain and use information collected or generated by others.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Single-Cell Analysis: Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.Gram-Negative Chemolithotrophic Bacteria: A large group of bacteria including those which oxidize ammonia or nitrite, metabolize sulfur and sulfur compounds, or deposit iron and/or manganese oxides.Expeditions: Usually refers to planned scientific data-gathering excursions.Microspheres: Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.

Bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. (1/121)

An artesian sulfide- and sulfur-rich spring in southwestern Oklahoma is shown to sustain an extremely rich and diverse microbial community. Laboratory incubations and autoradiography studies indicated that active sulfur cycling is occurring in the abundant microbial mats at Zodletone spring. Anoxygenic phototrophic bacteria oxidize sulfide to sulfate, which is reduced by sulfate-reducing bacterial populations. The microbial community at Zodletone spring was analyzed by cloning and sequencing 16S rRNA genes. A large fraction (83%) of the microbial mat clones belong to sulfur- and sulfate-reducing lineages within delta-Proteobacteria, purple sulfur gamma-Proteobacteria, epsilon -Proteobacteria, Chloroflexi, and filamentous Cyanobacteria of the order Oscillatoria as well as a novel group within gamma-Proteobacteria. The 16S clone library constructed from hydrocarbon-exposed sediments at the source of the spring had a higher diversity than the mat clone library (Shannon-Weiner index of 3.84 compared to 2.95 for the mat), with a higher percentage of clones belonging to nonphototrophic lineages (e.g., Cytophaga, Spirochaetes, Planctomycetes, Firmicutes, and Verrucomicrobiae). Many of these clones were closely related to clones retrieved from hydrocarbon-contaminated environments and anaerobic hydrocarbon-degrading enrichments. In addition, 18 of the source clones did not cluster with any of the previously described microbial divisions. These 18 clones, together with previously published or database-deposited related sequences retrieved from a wide variety of environments, could be clustered into at least four novel candidate divisions. The sulfate-reducing community at Zodletone spring was characterized by cloning and sequencing a 1.9-kb fragment of the dissimilatory sulfite reductase (DSR) gene. DSR clones belonged to the Desulfococcus-Desulfosarcina-Desulfonema group, Desulfobacter group, and Desulfovibrio group as well as to a deeply branched group in the DSR tree with no representatives from cultures. Overall, this work expands the division-level diversity of the bacterial domain and highlights the complexity of microbial communities involved in sulfur cycling in mesophilic microbial mats.  (+info)

Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. (2/121)

Two thermophilic, Gram-negative, non-spore-forming, multicellular filamentous micro-organisms were isolated from thermophilic granular sludge in an upflow anaerobic sludge blanket reactor treating fried soybean-curd manufacturing waste water (strain UNI-1(T)) and from a hot spring sulfur-turf in Japan (strain STL-6-O1(T)). The filaments were longer than 100 microm and of 0.2-0.3 microm (strain UNI-1(T)) or 0.7-0.8 microm (strain STL-6-O1(T)) in width. Strain UNI-1(T) was a strictly anaerobic organism. The optimum temperature for growth was around 55 degrees C; growth occurred in the range 50-60 degrees C. The optimum pH for growth was around 7.0; growth occurred in the range pH 6.0-8.0. Strain STL-6-O1(T) was a facultatively aerobic bacterium. The optimum temperature for growth was around 55 degrees C; growth occurred in the range 37-65 degrees C. The optimum pH for growth was around 7.5-8.0; growth occurred in the range pH 7.0-9.0. The two organisms grew chemo-organotrophically on a number of carbohydrates and amino acids in the presence of yeast extract. The G+C content of the DNA of strains UNI-1(T) and STL-6-O1(T) was 54.5 and 59.0 mol%, respectively. Major cellular fatty acids for strain UNI-1(T) were C(16 : 0), C(15 : 0), C(14 : 0) and C(18 : 0), whereas those for strain STL-6-O1(T) were C(18 : 0), C(16 : 0), C(17 : 0) and iso-C(17 : 0). MK-10 was the major quinone from aerobically grown STL-6-O1(T) cells. Phylogenetic analyses based on 16S rDNA sequences revealed that both strains belong to an uncultured, previously recognized clone lineage of the phylum Chloroflexi (formerly known as green non-sulfur bacteria). These phenotypic and genetic properties suggested that each strain should be classified into a new independent genus; hence, the names Anaerolinea thermophila and Caldilinea aerophila are proposed for strains UNI-1(T) (=JCM 11387(T)=DSM 14523(T)) and STL-6-O1(T)(=JCM 11388(T)=DSM 14525(T)), respectively. These strains represent the type and sole species of the genera Anaerolinea and Caldilinea, respectively.  (+info)

Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean. (3/121)

Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi: While members of this diverse group are consistently identified in the marine environment, there are currently no cultured representatives, and very little is known about their distribution or abundance in the world's oceans. In this study, published and newly identified SAR202-related 16S rRNA gene sequences were used to further resolve the phylogeny of this cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. Direct cell counts from the Bermuda Atlantic time series study site in the north Atlantic Ocean, the Hawaii ocean time series site in the central Pacific Ocean, and along the Newport hydroline in eastern Pacific coastal waters showed that SAR202 cluster cells were most abundant below the deep chlorophyll maximum and that they persisted to 3600 m in the Atlantic Ocean and to 4000 m in the Pacific Ocean, the deepest samples used in this study. On average, members of the SAR202 group accounted for 10.2% (+/-5.7%) of all DNA-containing bacterioplankton between 500 and 4000 m.  (+info)

A gene from the mesophilic bacterium Dehalococcoides ethenogenes encodes a novel mannosylglycerate synthase. (4/121)

Mannosylglycerate (MG) is a common compatible solute found in thermophilic and hyperthermophilic prokaryotes. In this study we characterized a mesophilic and bifunctional mannosylglycerate synthase (MGSD) encoded in the genome of the bacterium Dehalococcoides ethenogenes. mgsD encodes two domains with extensive homology to mannosyl-3-phosphoglycerate synthase (MPGS, EC 2.4.1.217) and to mannosyl-3-phosphoglycerate phosphatase (MPGP, EC 3.1.3.70), which catalyze the consecutive synthesis and dephosphorylation of mannosyl-3-phosphoglycerate to yield MG in Pyrococcus horikoshii, Thermus thermophilus, and Rhodothermus marinus. The bifunctional MGSD was overproduced in Escherichia coli, and we confirmed the combined MPGS and MPGP activities of the recombinant enzyme. The optimum activity of the enzyme was at 50 degrees C. To examine the properties of each catalytic domain of MGSD, we expressed them separately in E. coli. The monofunctional MPGS was unstable, while the MPGP was stable and was characterized. Dehalococcoides ethenogenes cannot be grown sufficiently to identify intracellular compatible solutes, and E. coli harboring MGSD did not accumulate MG. However, Saccharomyces cerevisiae expressing mgsD accumulated MG, confirming that this gene product can synthesize this compatible solute and arguing for a role in osmotic adjustment in the natural host. We did not detect MGSD activity in cell extracts of S. cerevisiae. Here we describe the first gene and enzyme for the synthesis of MG from a mesophilic microorganism and discuss the possible evolution of this bifunctional MGSD by lateral gene transfer from thermophilic and hyperthermophilic organisms.  (+info)

Populations implicated in anaerobic reductive dechlorination of 1,2-dichloropropane in highly enriched bacterial communities. (5/121)

1,2-Dichloropropane (1,2-D), a widespread groundwater contaminant, can be reductively dechlorinated to propene by anaerobic bacteria. To shed light on the populations involved in the detoxification process, a comprehensive 16S rRNA gene-based bacterial community analysis of two enrichment cultures derived from geographically distinct locations was performed. Analysis of terminal restriction fragments, amplicons obtained with dechlorinator-specific PCR primers, and enumeration with quantitative real-time PCR as well as screening clone libraries all implied that Dehalococcoides populations were involved in 1,2-D dechlorination in both enrichment cultures. Physiological traits (e.g., dechlorination in the presence of ampicillin and a requirement for hydrogen as the electron donor) supported the involvement of Dehalococcoides populations in the dechlorination process. These findings expand the spectrum of chloroorganic compounds used by Dehalococcoides species as growth-supporting electron acceptors. The combined molecular approach allowed a comparison between different 16S rRNA gene-based approaches for the detection of Dehalococcoides populations.  (+info)

Recommended standards for the description of new species of anoxygenic phototrophic bacteria. (6/121)

Recommended standards for the description of new species of the anoxygenic phototrophic bacteria are proposed in accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria. These standards include information on the natural habitat, ecology and phenotypic properties including morphology, physiology and pigments and on genetic information and nucleic acid data. The recommended standards were supported by the Subcommittee on the taxonomy of phototrophic bacteria of the International Committee on Systematics of Prokaryotes. They are considered as guidelines for authors to prepare descriptions of new species.  (+info)

Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. (7/121)

Reductive dehalogenation of vinyl chloride (VC) to ethene is the key step in complete anaerobic degradation of chlorinated ethenes. VC-reductive dehalogenase was partially purified from a highly enriched culture of the VC-respiring Dehalococcoides sp. strain VS. The enzyme reduced VC and all dichloroethene (DCE) isomers, but not tetrachloroethene (PCE) or trichloroethene (TCE), at high rates. By using reversed genetics, the corresponding gene (vcrA) was isolated and characterized. Based on the predicted amino acid sequence, VC reductase is a novel member of the family of corrinoid/iron-sulfur cluster containing reductive dehalogenases. The vcrA gene was found to be cotranscribed with vcrB, encoding a small hydrophobic protein presumably acting as membrane anchor for VC reductase, and vcrC, encoding a protein with similarity to transcriptional regulators of the NosR/NirI family. The vcrAB genes were subsequently found to be present and expressed in other cultures containing VC-respiring Dehalococcoides organisms and could be detected in water samples from a field site contaminated with chlorinated ethenes. Therefore, the vcrA gene identified here may be a useful molecular target for evaluating, predicting, and monitoring in situ reductive VC dehalogenation.  (+info)

Dominant microbial composition and its vertical distribution in saline meromictic Lake Kaiike (Japan) as revealed by quantitative oligonucleotide probe membrane hybridization. (8/121)

Vertical distributions of dominant bacterial populations in saline meromictic Lake Kaiike were investigated throughout the water column and sediment by quantitative oligonucleotide probe membrane hybridization. Three oligonucleotide probes specific for the small-subunit (SSU) rRNA of three groups of Chlorobiaceae were newly designed. In addition, three general domain (Bacteria, Archaea, and Eukarya)-specific probes, two delta-Proteobacteria-specific probes, a Chlorobiaceae-specific probe, and a Chloroflexi-specific probe were used after optimization of their washing conditions. The abundance of the sum of SSU rRNAs hybridizing with probes specific for three groups of Chlorobiaceae relative to total SSU rRNA peaked in the chemocline, accounting for up to 68%. The abundance of the delta-proteobacterial SSU rRNA relative to total SSU rRNA rapidly increased just below the chemocline up to 29% in anoxic water and peaked at the 2- to 3-cm sediment depth at ca. 34%. The abundance of SSU rRNAs hybridizing with the probe specific for the phylum Chloroflexi relative to total SSU rRNA was highest (31 to 54%) in the top of the sediment but then steeply declined with depth and became stable at 11 to 19%, indicating the robust coexistence of sulfate-reducing bacteria and Chloroflexi in the top of the sediment. Any SSU rRNA of Chloroflexi in the water column was under the detection limit. The summation of the signals of group-specific probes used in this study accounted for up to 89% of total SSU rRNA, suggesting that the DGGE-oligonucleotide probe hybridization approach, in contrast to conventional culture-dependent approaches, was very effective in covering dominant populations.  (+info)

Author(s): Zhuang, Wei-Qin; Yi, Shan; Bill, Markus; Brisson, Vanessa L; Feng, Xueyang; Men, Yujie; Conrad, Mark E; Tang, Yinjie J; Alvarez-Cohen, Lisa | Abstract: The acetyl-CoA Wood-Ljungdahl pathway couples the folate-mediated one-carbon (C1) metabolism to either CO2 reduction or acetate oxidation via acetyl-CoA. This pathway is distributed in diverse anaerobes and is used for both energy conservation and assimilation of C1 compounds. Genome annotations for all sequenced strains of Dehalococcoides mccartyi, an important bacterium involved in the bioremediation of chlorinated solvents, reveal homologous genes encoding an incomplete Wood-Ljungdahl pathway. Because this pathway lacks key enzymes for both C1 metabolism and CO2 reduction, its cellular functions remain elusive. Here we used D. mccartyi strain 195 as a model organism to investigate the metabolic function of this pathway and its impacts on the growth of strain 195. Surprisingly, this pathway cleaves acetyl-CoA to donate a methyl group for
Different Dehalococcoides strains contain different numbers of rdh genes that encode protein, which have been proven or predicted to catalyse the dechlorination reaction. When compared with the genomes of other dechlorinating bacteria, Dehalococcoides have the highest number of rdh genes in their genomes (Table 3). Genomes of strains 195, CBDB1 and BAV1 have 17, 32 and 10 rdh genes, respectively, whereas only seven rdh genes were identified in the genome of Desulfitobacterium hafniense DCB-2, four rdh genes in D. hafniense Y51 and two rdh genes in Geobacter lovleyi SZ and Anaeromyxobacter dehalogenans (Thomas et al., 2008). The draft genome of strain VS contains the highest number of rdh genes (36 full-length genes) ever found in a single bacterial genome (McMurdie et al., 2008). Similarly, 14 and 19 rdh genes were detected via PCR amplification in Dehalococcoides sp. strains FL2 and DCMB5 respectively (Holscher et al., 2004; Bunge et al., 2008). Twelve rdh genes from strain CBDB1 have ...
Reductive dehalogenases are the critical enzymes for anaerobic organohalide respiration, a microbial metabolic process that has been harnessed for bioremediation efforts to resolve chlorinated solvent contamination in groundwater and is implicated in the global halogen cycle. Reductive dehalogenase sequence diversity is informative for the dechlorination potential of the site or enrichment culture. A suite of degenerate PCR primers targeting a comprehensive curated set of reductive dehalogenase genes was designed and applied to twelve DNA samples extracted from contaminated and pristine sites, as well as six enrichment cultures capable of reducing chlorinated compounds to non-toxic end-products. The amplified gene products from four environmental sites and two enrichment cultures were sequenced using Illumina HiSeq, and the reductive dehalogenase complement of each sample determined. The results indicate that the diversity of the reductive dehalogenase gene family is much deeper than is currently
TY - JOUR. T1 - Seasonal dominance of CL500-11 bacterioplankton (phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan. AU - Okazaki, Yusuke. AU - Hodoki, Yoshikuni. AU - Nakano, Shin Ichi. PY - 2013/1/1. Y1 - 2013/1/1. N2 - Uncultured bacteria affiliated with the CL500-11 cluster (phylum Chloroflexi) were first reported from the oxygenated hypolimnion of Crater Lake (USA) as a predominant bacterioplankton, although this dominance has not been reported in other environments. In this study, we showed that CL500-11 is also dominant in the oxygenated hypolimnion of Lake Biwa (Japan) and followed its spatiotemporal succession using fluorescent in situ hybridization. CL500-11 cells were almost absent [, 1% of 4′,6-diamidino-2-phenylindole (DAPI)-stained cells] at the beginning of the stratification period, dominated (, 10% of DAPI-stained cells; maximum = 16.5%) in the hypolimnion during the stratification period, and decreased to below the detection limit with the collapse of the ...
Regarding the class "Dehalococcoidetes", the placeholder name was given by Hugenholtz & Stackebrandt, 2004,[6] after "Dehalococcoides ethenogenes" a partially described species in 1997,[7] whereas the first species fully described was Dehalogenimonas lykanthroporepellens by Moe et al. 2009,[8] but in the description of that species the class was not made official nor were families or orders laid out as the two species share only 90% 16S identity, meaning that they could fall in different families or even orders.[8] Recent phylogenetic analysis of the Chloroflexi has found very weak support for the grouping together of the different classes currently part of the phylum.[9] The six classes that make up the phylum did not consistently form a well-supported monophyletic clade in phylogenetic trees based on concatenated sequences for large datasets of proteins and no conserved signature indels were identified that were uniquely shared by the entire phylum.[9] However, the classes "Chloroflexi" and ...
Dehalococcoidetes" is a placeholder name given by Hugenholtz & Stackebrandt, 2004,[7] after "Dehalococcoides ethenogenes" a species partially described in 1997.[8] The first species fully described was Dehalogenimonas lykanthroporepellens, by Moe et al. 2009,[9] but in the description of that species the class was not made official nor were families or orders laid out as the two species share only 90% 16S ribosomal RNA identity, meaning that they could fall in different families or even orders.[9]. Recent phylogenetic analysis of the Chloroflexi has found very weak support for the grouping together of the different classes currently part of the phylum.[10] The six classes that make up the phylum did not consistently form a well-supported clade in phylogenetic trees based on concatenated sequences for large datasets of proteins, and no conserved signature indels were identified that were uniquely shared by the entire phylum.[10] However, the classes Chloroflexi and Thermomicrobia were found to ...
This study explored the potential of eutrophic river sediments to attenuate the infiltration of chlorinated aliphatic hydrocarbon (CAH)-polluted groundwater discharging into the Zenne River near Brussels, Belgium. Active biotic reductive dechlorination of CAHs in the riverbed was suggested by a high dechlorination activity in batch- and column biodegradation tests performed with sediment samples, and by the detection of dechlorination products in sediment pore water. Halorespiring Dehalococcoides spp. were present in large numbers in the riverbed as shown by quantification of their 16S rRNA and reductive dehalogenase genes. By using DGGE-fingerprint analysis of relevant nucleic acid markers, it was shown that the Zenne River sediments were inhabited by a metabolically diverse bacterial community. A large diversity of sulfate-reducing bacteria, Geobacteraceae and methanogens, which potentially compete with halorespiring bacteria for electron resources, was identified. The high organic carbon ...
This study explored the potential of eutrophic river sediments to attenuate the infiltration of chlorinated aliphatic hydrocarbon (CAH)-polluted groundwater discharging into the Zenne River near Brussels, Belgium. Active biotic reductive dechlorination of CAHs in the riverbed was suggested by a high dechlorination activity in batch- and column biodegradation tests performed with sediment samples, and by the detection of dechlorination products in sediment pore water. Halorespiring Dehalococcoides spp. were present in large numbers in the riverbed as shown by quantification of their 16S rRNA and reductive dehalogenase genes. By using DGGE-fingerprint analysis of relevant nucleic acid markers, it was shown that the Zenne River sediments were inhabited by a metabolically diverse bacterial community. A large diversity of sulfate-reducing bacteria, Geobacteraceae and methanogens, which potentially compete with halorespiring bacteria for electron resources, was identified. The high organic carbon ...
Enrichment cultures are investigated for the bioremediation potential of tetrachloroethene (PCE). This process is based on anaerobic respiration in which chlorinated compounds are used as electron acceptors (organohalide respiration, OHR). The key enzyme in OHR is the reductive dehalogenase (RdhA). SL2, a culture dechlorinating PCE to harmless ethene, was shown to have a small number of OHR bacteria, mainly Sulfurospirillum spp. and Dehalococcoides spp. (1). Aim: We aim at a better understanding of the functional diversity of rdhA genes in the SL2 culture and their dynamic during PCE degradation. Methods: By applying different frequencies of culture transfer, 3 sub-cultures were obtained from the SL2 enrichment showing different degradation patterns. The degradation profile was followed by gas chromatography and chloride titration. DNA and RNA were also extracted, PCR and qPCR was performed for the detection of rdhA genes belonging to Sulfurospirillum. Besides, a T-RFLP method was developed to ...
Molecular data show that the filamentous bacterium Eikelboom type 0092, frequently seen in Australian activated sludge plants, is a member of the phylum Chloroflexi. Fluorescence in situ hybridization (FISH) probes designed against cloned 16S rRNA sequences from a full-scale enhanced biological phosphate removal-activated sludge plant community, where this was a dominant filament morphotype, suggest that it can exist as two variants, differing in their trichome diameter. When applied to samples from several treatment plants in eastern Australia, each FISH probe targeted only the type 0092 filament morphotype against which it was designed. The patterns of FISH signals generated with both were consistent with the ribosomes not being evenly distributed but arranged as intracellular aggregates. The FISH survey data showed that these two variants appeared together in most but not all of the plants examined. None stained positively for intracellular presence of either poly-beta-hydroxyalkanoates or ...
Polychlorinated aliphatic C2 and C3 alkanes (ethanes and propanes with at least two chlorine substituents) are industrially important chemical intermediates globally produced on a massive scale.[11] Due to spills and past inappropriate disposal methods, these chlorinated compounds are prevalent groundwater and soil contaminants throughout the US and around the world.[11] Bioremediation approaches that rely on the action of anaerobic, reductively-dehalogenating bacteria, such as D. lykanthroporepellens, have shown great promise for clean-up of chlorinated solvent-contaminated soil and groundwater.[4] Using qPCR (quantitative real-time polymerase chain reaction), 16S rRNA gene sequences for Dehalogenimonas strains have been found to be at concentrations as high as 106 copies/ml of groundwater contaminated with high concentrations of chlorinated solvents and comprise up to nearly 19% of the total bacterial 16S rRNA gene copies.[12] The characterization of D. lykanthroporepellens has aided in ...
Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized to clarify their taxonomic position. The two strains, designated BL-DC-8 and BL-DC-9T, were mesophilic, non-spore-forming, non-motile, Gram-negative staining and strictly anaerobic. Cells were irregular cocci, 0.3-0.6 μm in diameter. The two strains were resistant to ampicillin and vancomycin. Hydrogen was utilized as an electron donor. The genomic DNA G+C content of strains BL-DC-8 and BL-DC-9T was 54.0 and 53.8 mol%, respectively. The major cellular fatty acids were C18 : 1 ω9c, C16 : 1 ω9c, C16 : 0 and C14 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the strains cluster within the phylum Chloroflexi, but are related only distantly to all recognized taxa in the phylum. Morphological, physiological and
SiREM offers a growing number of targets for Gene-Trac® testing that includes: Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg), Desulfitobacterium (Dsb) and key functional genes such as vinyl chloride reductase (vcrA, bvcA), trichloroethene reductase (tceA), chloroform, 1,1,1-TCA and 1,1-DCA reductase (cfrA/dcrA). These microorganisms and functional genes are critical to reductive dechlorination of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes, and propanes. Geobacter and sulfate reducers play key roles in the metabolism of aromatic hydrocarbon compounds and the generation of iron sulfur compounds critical to natural attenuation remedies. The dxmB and ALDH genes are important in the aerobic degradation of 1,4-dioxane.. Gene-Trac® quantitative polymerase chain reaction (qPCR) testing is used to quantify key dechlorinating bacteria and functional genes. If a comprehensive analysis of microbial community is required Gene-Trac® NGS (next ...
The discovery of Dehalococcoides mccartyi reducing perchloroethene and trichloroethene (TCE) to ethene was a key landmark for bioremediation applications at contaminated sites. D. mccartyi-containing cultures are typically grown in batch-fed reactors. On the other hand, continuous cultivation of these microorganisms has been described only at long hydraulic retention times (HRTs). We report the cultivation of a representative D. mccartyi-containing culture in continuous stirred-tank reactors (CSTRs) at a short, 3-d HRT, using TCE as the electron acceptor. We successfully operated 3-d HRT CSTRs for up to 120 days and observed sustained dechlorination of TCE at influent concentrations of 1 and 2 mM TCE to ≥97 % ethene, coupled to the production of 1012 D. mccartyi cells Lculture −1. These outcomes were possible in part by using a medium with low bicarbonate concentrations (5 mM) to minimize the excessive proliferation of microorganisms that use bicarbonate as an electron acceptor and compete with D.
Photosynthetic organisms have evolved diverse antennas to harvest light of various qualities and intensities. Anoxygenic phototrophs can have bacteriochlorophyll Qy antenna absorption bands ranging from about 700-1100 nm. This broad range of usable wavelengths has allowed many organisms to thrive in unique environments. Roseiflexus castenholzii is a niche-adapted, filamentous anoxygenic phototroph: FAP) that lacks chlorosomes, the dominant antenna found in all green bacteria. Light-harvesting is realized only in the membrane with BChl a and a variety of carotenoids. Through biochemical and spectroscopic methods, a model for the size and organization of the photosynthetic antenna is presented. Despite the wide distribution of antennas, photochemistry occurs in the reaction center: RC), which can be separated into two groups distinguishable by the identity of the terminal electron acceptor. These are the Fe-S type or type-I and the quinone-type or type -II RCs. All known anoxygenic phototrophs have
ទីតាំងជាទូទៅនៃការទទួលយកបានភាគច្រើនជា root នៃ មែកធាងនៃជីវិត គឺរវាង monophyletic ដែន បាក់តេរី និង clade បានបង្កើតឡើងដោយ អាកឃី និង ការី នៃអ្វីដែលត្រូវបានសំដៅដល់ថាជា«ដើមឈើប្រពៃណីនៃជីវិត "ដោយផ្អែកលើការសិក្សាម៉ូលេគុលជាច្រើន។ [៣៩] [៤០] [៤១] [៤២] [៤៣] [៤៤] មួយជនជាតិភាគតិចតូចណាស់នៃការសិក្សាបានសន្និដ្ឋានខុសគ្នាគឺថាជា root គឺស្ថិតនៅក្នុងបាក់តេរីដែនទាំងនៅក្នុង phylum នេះ Firmicutes [៤៥] ឬថា phylum នេះ Chloroflexi គឺ ...
Obradović, Davor, et al. "A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160." PloS one 11.7 (2016): e0159231.. Hemp, James, et al. "Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. "Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. "Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae." Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. "Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia." Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. "Draft Genome of ...
Test kit for rapid identification and quantification of the problem-causing Chloroflexi as well as Eikelboom Type 1851 filaments in wastewater samples.
JPT Peptide Technologies is a DIN ISO 9001:2015 certified and GCLP compliant integrated provider of innovative peptide based catalog products and custom services.
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Fullerton, H. and C. L. Moyer. 2016. Comparative single-cell genomics of Chloroflexi from the Okinawa Trough deep subsurface biosphere. Appl. Environ. Microbiol. 82:3000-3008.. Methodology from Fullerton and Moyer (2016). See paper for references cited below.. Sample collection. Subsurface sediments were collected on IODP expedition 331 (Deep Hot Biosphere) from 1 September through 4 October 2010 (Fig. 1). Onboard contamination testing of sites C0015 (126°53=E, 27°47=N; hole B; section 1H-5; 5.6mbsf) and C0017 (126°55=E, 27°47=N; hole C; section 1H-7; 26.6 m bsf) found no indication of interior-core contamination using fluorescent microspheres (both holes C0015B and C0017C) and perfluorocarbon tracer (hole C0017C only). The sample from hole C0017C was also verified by PCR-generated phylotype comparisons based on 97% similarity to phylotypes obtained from drilling mud at a contamination level of 1% or less (26). Subsamples were aseptically collected from the interiors of whole-round cores and ...
All groups of dioxin-like compounds are persistent in the environment.[61] Very few soil microbes nor animals are able to break down effectively the PCDD/Fs with lateral chlorines (positions 2,3,7, and 8). This causes very slow elimination. However scientists at Martin Luther University recently found that a type of bacteria Dehalococcoides CBDB1 can extract the chlorine from dioxin compounds in the absence of oxygen.[62][63] Ultraviolet light is able to slowly break down these compounds. Lipophilicity (tendency to seek for fat-like environments) and very poor water solubility make these compounds move from water environment to living organisms having lipid cell structures. This is called bioaccumulation. Increase in chlorination increases both stability and lipophilicity. The compounds with the very highest chlorine numbers (e.g. octachlorodibenzo-p-dioxin) are, however, so poorly soluble that this hinders their bioaccumulation.[61] Bioaccumulation is followed by biomagnification. Lipid-soluble ...
TY - BOOK. T1 - Enhanced reductive dechlorination in clay till contaminated with chlorinated solvents. AU - Damgaard,Ida. A2 - Broholm,Mette Martina. A2 - Bjerg,Poul Løgstrup. PY - 2012. Y1 - 2012. N2 - Chlorinated solvents are among the most frequently found contaminants in groundwater. In fractured media, chlorinated ethenes and ethanes are transported downwards through preferential pathways with subsequent diffusion into the sediment matrix. Due to slow back diffusion it can serve as a long term secondary source that can leach to the underlying aquifer. As some of the chlorinated solvents and their degradation products are toxic and carcinogenic, remediation technologies applicable in low permeability settings are needed. Enhanced reductive dechlorination (ERD) has been proven efficient in high permeability aquifers and has also been applied at a number of low permeability clay till sites. This thesis presents the results of an investigation of chlorinated ethenes (and ethanes) degradation ...
Sutcliffe, Iain C. (2010). "A phylum level perspective on bacterial cell envelope architecture". Trends in Microbiology 18 (10): 464-470. doi:10.1016/j.tim.2010.06.005. ISSN 0966842X. ...
Can the Acacia Strain pull off one final victory to cement their place in the Death Match Hall of Fame? Or will Ill Nino snatch away a win to begin their own trail to the Hall? The choice is yours. Vote for your favorite track below!. (This Death Match ends on Tuesday, Nov. 13, at 10AM ET. Fans can vote once per hour! So come back and vote often to make sure your favorite song wins!) ...
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
In our research we are developing new isotope and microscopy techniques for the investigation of biogeochemical processes. We want to understand the anaerobic microbiological processes involved in the natural attenuation of hydrocarbons and halogenated organic compounds and the new "emerging contaminants" in various ecosystems on the microbiological, biochemical and genetic level in order to quantify ecosystems services. For example, we aim to fundamentally understand key degraders such as Dehalococcoides mccartyi involved in the detoxification of organohalides and synthrophic interactions in microbial communities leading to hydrocarbon removal and the biogeochemical cycles of C, N, P, S and various metals in terrestrial systems. We analyse microbially-mediated corrosion to mitigate damage to gas and oil infrastructure. Knowledge of mineral corrosion helps us to develop bioleaching processes with which to extract metals and rare earth elements ...
Do you suffer from symptoms after eating? Such as digestive symptoms, foggy brain, sinus congestion or aches and pains. Do you notice it after gluten containing foods or just cant track whats causing it? Chances are youre body is reacting to components in plants called lectins.. What are lectins? So happy you asked!. Lectins are the plants natural compounds to ward off pests, fungal and bacterial attack. That is, the plants natural immune system. When plants are under attack they raise their lectin numbers to fend off the attacking pests. This is the system by which pesticides work. However, pests are gaining tolerance resulting in needing an increase of pesticide to be applied to the plant and thus a further increase of the plants lectins.. Modern wheat strains vs old style grains. Our modern wheat strains have been modified to produce more lectins to be highly pest resistant. This very clever genetically engineering won Norman Borlaug a Nobel peace prize in 1970 and changed the industry ...
It can be hard to tell the difference between a groin strain (muscle or tendon tear) and an inguinal hernia (fat or intestine poking through a hole in the…
From genomic libraries of purple sulphur bacteria, fragments were cloned that encoded for proteins involved in the synthesis of poly(3-hydroxyalkanoic acids), PHA. A 12.5- and a 15.0- plus a 15.6-kbp
Rabbit monoclonal antibody raised against a human TCEA3 peptide using ARM Technology. A synthetic peptide of human TCEA3 is used for rabbit immunization.Customer or Abnova will decide on the preferred peptide sequence. (H00006920-K) - Products - Abnova
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.. ...
Thompson LR, Sanders JG, McDonald D, and the Earth Microbiome Project Consortium including Seyler LM, and Kerkhof LJ. 2017. A communal catalogue reveals Earths multiscale microbial diversity. Nature. doi: 10.1038/nature24621. Liu, J., Lopez, N., Ahn, Y., Goldberg, T., Bromberg, Y., Kerkhof, L. J. and Häggblom, M. M. 2017. Novel reductive dehalogenases from the marine sponge associated bacterium Desulfoluna spongiiphila. Environmental Microbiology Reports. doi:10.1111/ 1758-2229.12556. Rodenburg LA, Dewani Y, Häggblom MM, Kerkhof LJ, and Fennell DE. 2017. Forensic Analysis of Polychlorinated Dibenzo-p-Dioxin and Furan Fingerprints to Elucidate Dechlorination Pathways. Environ. Sci. Technol. doi: 10.1021/acs.est.7b02705. Kerkhof LJ, Dillon KP, Häggblom MM, McGuinness LR. 2017. Profiling bacterial communities by MinION sequencing of ribosomal operons. Microbiome. doi : 10.1186/s40168-017-0336. Hotaling, L. McDonnell, J., Ferraro, C., Litchenwalner, C.S., and Florio, K. (2017). Educating with ...
Methodology/Principal Findings: Here, we report molecular monitoring of the stone-autochthonous microbiota before and at 5, 12 and 30 months after the bio-consolidation treatment (medium/long-term monitoring), employing the well-known molecular strategy of DGGE analyses. Before the bio-consolidation treatment, the bacterial diversity showed the exclusive dominance of Actinobacteria (100%), which decreased in the community (44.2%) after 5 months, and Gamma-proteobacteria (30.24%) and Chloroflexi (25.56%) appeared. After 12 months, Gamma-proteobacteria vanished from the community and Cyanobacteria (22.1%) appeared and remained dominant after thirty months, when the microbiota consisted of Actinobacteria (42.2%) and Cyanobacteria (57.8%) only. Fungal diversity showed that the Ascomycota phylum was dominant before treatment (100%), while, after five months, Basidiomycota (6.38%) appeared on the stone, and vanished again after twelve months. Thirty months after the treatment, the fungal population ...
Chlorinated compounds such as trichloroethene (TCE) are recalcitrant contaminants commonly detected in soil and groundwater. Contemporary remedies such as electron donor amendment tend to be less or ineffective in treating chlorinated compounds in ma
uuid": "e4ad4307-37a1-4183-beec-921ec07a361c", "type": "records", "etag": "6cb890cbdb5221e54b3677b2174a3b0f4be4b3a9", "data": { "dwc:startDayOfYear": "211", "dwc:specificEpithet": "ilicina", "dwc:kingdom": "Fungi", "dwc:recordedBy": "I.M. Brodo; W.B. Schofield", "dwc:order": "Arthoniales", "dwc:habitat": "along a shallow stream and shore in Thuja-Alnus forest; at edge of beach; Alnus sinuata", "dwc:scientificNameAuthorship": "Taylor", "dwc:occurrenceID": "e4ad4307-37a1-4183-beec-921ec07a361c", "id": "69977", "dwc:stateProvince": "British Columbia", "dwc:eventDate": "1967-07-30", "dwc:collectionID": "ca9232e9-9401-4c90-9845-d00fe37f0ac2", "dwc:country": "Canada", "idigbio:recordId": "urn:uuid:e4ad4307-37a1-4183-beec-921ec07a361c", "dwc:decimalLatitude": "53.6333333", "dwc:basisOfRecord": "PreservedSpecimen", "dwc:genus": "Arthonia", "dwc:family": "Arthoniaceae", "dc:rights": "http://creativecommons.org/licenses/by-nc/3.0/", "dwc:identifiedBy": "T.L. Esslinger: Checklist of North America, 2001", ...
Invertebrates - Ecophysiology and Management. Edited by: Sajal Ray, Genaro Diarte-Plata and Ruth Escamilla-Montes. ISBN 978-1-83968-551-4, eISBN 978-1-83968-552-1, PDF ISBN 978-1-83968-553-8, Published 2020-01-22
Hoogewijs, D. , Terwilliger, N. B. , Webster, K. A. , Powell-Coffman, J. A. , Tokishita, S. , Yamagata, H. , Hankeln, T. , Burmester, T. , Rytkönen, K. T. , Nikinmaa, M. , Abele, D. , Heise, K. , Lucassen, M. , Fandrey, J. , Maxwell, P. H. , Pahlman, S. and Gorr, T. A. (2007 ...
As the Regions only Level 1 Trauma and Burn center, the Upstate Golisano Childrens Hospital offers a complete range of health care services for children from birth to 19 years of age. Our dedicated pediatric providers and staff care for patients in an inpatient setting that consists of General Pediatrics, Pediatric Surgery Unit, Hematology/ Oncology, and the only Pediatric Intensive Care Unit in our large referral area and the only dedicated pediatric Emergency Department between Rochester and Vermont. In addition, the Childrens Hospital offers full spectrum of ambulatory services including primary care and several nationally recognized specialty medical care programs.. Browse the Pediatric Services and Clinics A-Z list to find a comprehensive list of clinics providing services. Use the Find a Doctor link to get more information about a doctor or to search for doctors with a particular specialty or who treat specific diseases. ...
Recommended standards for the description of new species of the anoxygenic phototrophic bacteria are proposed in accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria. These standards include information on the natural habitat, ecology and phenotypic properties including morphology, physiology and pigments and on genetic information and nucleic acid data. The recommended standards were supported by the Subcommittee on the taxonomy of phototrophic bacteria of the International Committee on Systematics of Prokaryotes. They are considered as guidelines for authors to prepare descriptions of new species.
Background Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of organohalide respiration (OHR). Dehalobacter restrictus is an obligate OHR bacterium (OHRB) able to conserve energy with tetrachloroethene, but is unable to de novo synthesize corrinoids. Genome analysis of D. restrictus strain PER-K23 however revealed the presence of the complete corrinoid biosynthesis pathway. Objectives The aim of the present study is to understand the corrinoid metabolism of D. restrictus strain PER-K23 at the level of biosynthesis, regulation and transport and to compare it to contrasting situations in other Dehalobacter strains. Methods Genome analysis was performed with standard bioinformatic tools. Both transcriptomic and proteomic approaches were applied on D. restrictus strain PER-K23 cells cultivated in media with alternative corrinoid conditions. Gene expression was further addressed using targeted reverse transcription and quantitative PCR. Growth and dechlorination of D. ...
Our comparative survey of five red-layer communities in Yellowstone National Park suggests the presence of a diverse and distinct group of uncultured GNS-like bacteria, the closest known isolate of which is R. castenholzii, a red filamentous Bchl a-only-containing bacterium from a similar hot springs in Japan. This hypothesis was supported by16S rRNA phylogenetic studies, in vivo Bchl a absorption spectra comparisons, and morphologic assessment of each mat red-layer sample.. Within this new red GNS cluster, two distinct and well-supported phylogenetic subclusters emerge: YRL-A was most similar to Roseiflexus, and YRL-B was most similar to two unclassified 16S rRNA sequences originally retrieved from Octopus Spring, Yellowstone National Park (27). A potential third cluster that contains all Spray Geyser GNS-like clones represents a well-supported subgroup within YRL-B. While our analyses were based on a somewhat limited character set (353 usable characters out of 980 aligned positions), we ...
REGENESIS Remediation Technologies recently hosted a free-of-charge webinar, A Data-Intensive Review of Enhanced Reductive Dechlorination Projects for the ...
Article Modeling trichloroethene reduction in a hydrogen-based biofilm. We constructed a multispecies biofilm model for simultaneous reduction of trichloroethene (TCE) and nitrate (NO3−) in the biofilm of a H2-based membrane biofilm reactor (M...
The British Private Equity & Venture Capital Association (BVCA) is the industry body and public policy advocate for the private equity and venture capital industry in the UK.
Wolters Kluwer Health and Vocera Communications, (VCRA) have teamed to arm Halifax Health with a high-powered surveillance solution to advance its battle
They will be a download ecophysiology of northern spruce species the performance of blue to the one Also when they are to have this power on an older religion. Microsoft Compatibility Page Chromosome for this literature. PowerPoint 97-2003 Presentation?
Die Goethe-Universität ist eine forschungsstarke Hochschule in der europäischen Finanzmetropole Frankfurt. Lebendig, urban und weltoffen besitzt sie als Stiftungsuniversität ein einzigartiges Maß an Eigenständigkeit.
Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region.. ...
The aerial surface of plants, the phyllosphere, is colonized by numerous bacteria displaying diverse metabolic properties that enable their survival in this specific habitat. Recently, we reported on the presence of microbial rhodopsin harbouring bacteria on the top of leaf surfaces. Here, we report on the presence of additional bacterial populations capable of harvesting light as a means of supplementing their metabolic requirements. An analysis of six phyllosphere metagenomes revealed the presence of a diverse community of anoxygenic phototrophic bacteria, including the previously reported methylobacteria, as well as other known and unknown phototrophs. The presence of anoxygenic phototrophic bacteria was also confirmed in situ by infrared epifluorescence microscopy. The microscopic enumeration correlated with estimates based on metagenomic analyses, confirming both the presence and high abundance of these microorganisms in the phyllosphere. Our data suggest that the phyllosphere contains a ...
Chlorinated organic compounds are extensively employed in industry and agriculture. These chemicals are considered among the most severe public health issues, due to their widespread and uncontrolled release in the environment combined to the recalcitrance to degradation and tendency to bio-accumulate. 1,2-dichloroethane (1,2-DCA) is one of the most widespread chlorinated pollutants. This solvent is a key intermediate in the production of PVC. The public health concern and environmental safety issues related to 1,2-DCA, have fueled the scientific interested on the application of feasible and effective bioremediation strategies, on the identification of microorganisms able to efficiently and rapidly degrade it. 1,2-DCA can undergo microbial-mediated degradation through several aerobic or anaerobic metabolic processes. Due to the typical presence of this pollutant in anoxic environments research has been focused on the anaerobic dehalogenation process based on reductive dechlorination in which ...
Looking for online definition of dehalogenase in the Medical Dictionary? dehalogenase explanation free. What is dehalogenase? Meaning of dehalogenase medical term. What does dehalogenase mean?
The toxic and carcinogenic compound 1,2-dichloropropane (DCP) is widely used in industry and agriculture. DCP shows a low chemical reactivity. It is only moderately soluble in aqueous systems and almost recalcitrant to microbial degradation under aerobic conditions. As a consequence DCP accumulates in groundwater, sediments and soil, thus endangering humans and animals via the food chain. To efficiently transform DCP to harmless organic compounds microbial mixed cultures have been enriched from sediments and were subsequently transferred into a fluidized bed bioreactor. This process allowed a continuous anaerobic dechlorination of DCP to propene. Bioreactor processes using complex microbiota represent a promising technology for transformation of chlorinated compounds. However, the composition of the used population is usually unknown, hence hindering both optimization and control of the degradation process. Subject of this work was the analysis of the microbial diversity of the ...
Alkaline siliceous hot spring microbial mats in Yellowstone National Park are composed of two dominant phototropic groups, cyanobacteria and green non-sulfur-like bacteria (GNSLB). While cyanobacteria are thought to cross-feed low-molecular-weight organic compounds to support photoheterotrophic meta …
Venkatachalam K, Arzuaga X, Chopra N, Gavalas VG, Xu J, Bhattacharyya D, Hennig B, Bachas LG. 2008. Reductive dechlorination of 3,3,4,4-tetrachlorobiphenyl (PCB77) using palladium or palladium/iron nanoparticles and assessment of the reduction in toxic potency in vascular endothelial cells. J Hazard Mater 159(2-3):483-491. doi:10.1016/j.jhazmat.2008.02.109 PMID:18423858 PMCID:PMC3247144 ...
While it is always fun to see your materials and blogs tweeted and translated in to other languages. Truly, the reason I created and published the resource has little to do with international social media fame. It was ultimately a labor of love designed to demystify the model (as admittedly… it was even a little difficult for me to process and apply at first) and provide real world ideas for integration that anyone could digest.. But I feel like I can take this one step further… Truly the height of the SAMR model is about creating authentic learning experiences that draw from collaboration, online publishing, and even formative assessment. During the TCEA workshop, many thoughtful conversations were had amongst the attendees. In hindsight, I wish I had designated a scribe to document the insights, questions, and critical conversations to archive and share with others that were not able to attend.. The S.A.S.S.Y. SAMR workshop delivered at TCEA is officially over, BUT truly we never stop ...
In this study, we expand upon the biogeography of biological soil crusts (BSCs) and provide molecular insights into the microbial community and biochemical dynamics along the vertical BSC column structure, and across a transect of increasing BSC surface coverage in the central Mojave Desert, California. Next generation sequencing reveals a bacterial community profile that is distinct among BSCs in the southwestern United States. Distribution of major phyla in the BSC topsoils included Cyanobacteria (33 ± 8%), Proteobacteria (26 ± 6%), and Chloroflexi (12 ± 4%), with Phormidium being the numerically dominant genus. Furthermore, BSC subsurfaces contained Proteobacteria (23 ± 5%), Actinobacteria (20 ± 5%), and Chlorflexi (18 ± 3%), with an unidentified genus from Chloroflexi (AKIW781, order) being numerically dominant. Across the transect, changes in distribution at the phylum (p
The mutations identified thus generic cialis india far in human patients with tooth agenesis also affect these pathways. The principle of the test is a qualitative in-vitro-assay that contains test strips coated with parallel lines of 14 highly purified antigens. Mortality benefit of statin use in traumatic spinal cord injury: a retrospective analysis. Advanced imaging techniques as magnetic resonance imaging (MRI) are increasingly performed in the diagnostic workup of patients. Pathological findings in patients suffering from transitory ischemic attacks by means of using neuroradiological methods (CCT included) are a rare condition.. Cultivation and genomic, nutritional, and lipid biomarker characterization of Roseiflexus strains closely related to predominant in situ populations inhabiting Yellowstone hot spring microbial mats. The resulting relative alkylation indices (RAIs) were calculated in order to correlate these indices with the results of the guinea pig sensitization test data. ...
Table 3: Measurement results of the field study: tetrachloroethene in end-exhaled air in exposed (dry-cleaning workers) and nonexposed (control group) subjects ...
* found in: Diethanolamine 1mL, Decanoic Acid 1000ppm 1mL, 1-4-Dichloro-2-butene 1000ppm, n-Decane 1mL, Desmetryn 1000ppm 1mL, 1 - 3-Dichloropropane..
Buy Ecophysiology of Northern Spruce Species: (9780660179599): The Performance of Planted Seedlings: NHBS - Steven Grossnickle, NRC Research Press
Abstract: Tetrachloroethene (perchloroethylene, PCE) is used for metal cleaning and degreasing, in dry cleanings and as solvent in the chemical industry. In epidemiology studies, which analysed collectives with potential PCE exposure (primarily dry cleaners), increased hazards regarding some specific cancer localisations have been described. However, the findings are not uniform. In the study in hand, the literature on epidemiology regarding cancer research and PCE exposure issues has been extensively consolidated. Each study has been critically reviewed in order to determine the quality of data and methods respectively. Findings of the relevant studies have been summarized individually for specific cancer localisations. The available literature shows strong methodical restrictions (exposition assessment and confounding) and provides heterogeneous results. None of the studies is adequately expressive, and the epidemiologic evidence in its entirety is not suitable to convincingly demonstrate that ...
Pentachlorophenol (PCP), a xenobiocide used to preserve lumbers, is a major environmental pollutant in North America. In spite of an expected high resistance to biodegradation, a number of aquatic and soil bacteria can degrade PCP. In this study, we cloned, expressed and purified tetrachlorobenzoquinone reductase (PcpD), the second enzyme in the PCP biodegradation pathway in Sphingobium chlorophenolicum. PcpD, present mainly as a homo-trimer, exhibited low but statistically significant activity in the reduction of tetrachlorobenzoquinone to tetrachlorohydroquinone. The optimal pH for PcpD activity was 7.0. PcpD was stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. Because of the constitutive expression and relatively high catalytic efficiency of downstream enzyme tetrachlorohydroquinone reductive dehalogenase, tetrachlorohydroquinone was unlikely to accumulate in high concentrations, suggesting that PcpD would only be stimulated by tetrachlorohydroquinone
This data report provides a compilation of information developed over the last 6+ years by a multi-disciplinary, multi-institutional research team. The overall goal of this work has been to identify the biological, chemical, and physical factors that control rates of reductive dechlorination of DDE and DDMU in sediments of the Palos Verdes Shelf (.... Eganhouse, Robert P.; Pontolillo, James; Orem, William H.; Webster, Daniel M.; Hackley, Paul C.; Edwards, Brian D.; Rosenberger, Kurt; Dickhudt, Patrick; Sherwood, Christopher R.; Reinhard, Martin; Qin, Sujie; Dougherty, Jennifer; Hopkins, Gary; Marshall, Ian; Spormann, Alfred ...
Ray, W. H., Jain, S. K. and Salovey, R. (1975), On the modeling of bulk poly(vinyl chloride) reactors. J. Appl. Polym. Sci., 19: 1297-1315. doi: 10.1002/app.1975.070190510 ...
A train derailment in Paulsboro, NJ has caused potentially hazardous vinyl chloride to spill into local air and water, reports say. The derailment, caused by the collapse of a rail bridge in the...
After 22 years of study, and intense political maneuvering, the US Environmental Protection Agency has formally classified TCE (trichloroethene, also called trichloroethylene) as a carcinogen, as well as a non-cancer hazard to human health. Canada Environment Siskinds LLP 7 Oct 2011
The Flexible Phenotype attempts a true synthesis of animal physiology, behavior, and ecology by developing an empirical argument that describes the intimate connections between animal phenotype and environment. It starts with a synthesis of the principles guiding current research in ecophysiology, behavior, and ecology, illustrating each aspect with the detailed results of empirical work on as wide a range of organisms as possible.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
D3150 - 11 Standard Specification for Crosslinked and Noncrosslinked Poly(Vinyl Chloride) Heat-Shrinkable Tubing for Electrical Insulation , crosslinked poly(vinyl chloride) heat shrinkable tubing, electrical insulation, heat-shrinkable tubing, poly (vinyl chloride), poly(vinyl chloride) heat-shrinkable tubing,
#131 Vinyl chloride 0.05-2%#131La Vinyl chloride 0.25-54ppm#131L Vinyl chloride 0.1-6.6ppm#131LB Vinyl chloride 0.25-70ppm
A process is disclosed for the preparation of polymers and copolymers of yl choride, which comprises polymerizing vinyl chloride or copolymerizing vinyl chloride and monomers copolymerizable therewith in the presence of a peroxide having the general formula ##SPC1##Wherein R represents an alkyl- or an alkoxy-group having from 1-6 carbon atoms as an initiator. The initiator is used in an amount of 0.01 to 1.0% by weight, calculated on the monomer or monomer mixture. The foregoing peroxide may be used in combination with another structurally similar peroxide or with other peroxides also capable of polymerizing or copolymerizing vinyl chloride in an amount of 0.01 to 0.1% by weight, calculated on the monomer or monomer mixture.
See http://www.clu-in.org/products/newsltrs/gwc/gwc1200.htm#biodegradation for a description of in-situ biodegradation enhanced with the injection of lactate used to treat the residual source area of a large trichloroethylene plume. TCE is present in a sludge mixture due to the historical injection of waste into the basalt aquifer. For eight months lactate was injected 200 to 300 feet below ground surface. During reductive dechlorination, TCE is transformed to 1,2-dichloroethylene (DCE), then vinyl chloride (VC), and finally ethene, the desired end product. Of particular importance was the appearance of ethene simultaneously with VC, indicating that VC would not accumulate in the system. The success of the project in a complex fractured basalt aquifer may be a milestone both for fractured rock remediation and for the in-situ bioremediation of chlorinated solvent source areas. ...
D4726 - 15 Standard Specification for Rigid Poly(Vinyl Chloride) (PVC) Exterior-Profile Extrusions Used for Assembled Windows and Doors , color-hold guidelines, doors, exterior-profile extrusions, poly(vinyl chloride) (PVC), recycled plastic, windows,,
Vinyl Chloride is a particularly dangerous chemical, but few people really understand the harm this solvent can cause. Visit Shrader & Associates L.L.P. online to discover what vinyl chloride is and more.
The Vinyl Chloride Health Committee promotes further understanding of the potential health effects of vinyl chloride exposure by sponsoring new research and evaluating the information that is already available.
Vinyl chloride in nitrogen; Refer to the product ′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Creative-Proteomics offer cas 27398 VINYL CHLORIDE (D3, 98%) 250 UG/ML IN METHANOL-OD. We are specialized in manufacturing Stabel Isotope Labeled Analytical Standard products.
View Details about HS code 3904 for Polymers Vinyl Chloride Halogenated products. Check Indian HS classifications List under Harmonized system code heading 3904.
Sensidyne-Kitagawa detector tubes are single stroke detector tubes for easy, low cost spot detection of a wide range of hazardous gases and vapors#132SA Vinyl Chloride 0.05-1.0%
This statistic shows the value from EU and non-EU exports of rigid tubes, pipes and hoses of polymers of vinyl chloride in the United Kingdom between 2010 and 2019*.
INVERTEBRATESIntroduction:Animalia is the largest of the five kingdom system, with over one million species. It has an extreme variety of shapes, colors, and sizes. It is made up of eukaryotic, multicellular, heterotrophic organisms that are either aqu...
Buy Protocols in Lichenology (9783540411390): Culturing, Biochemistry, Ecophysiology and Use in Biomonitoring: NHBS - Edited By: I Kranner, R Beckett and A Varma, Springer-Verlag
Three decades of study of laminated mats in low-sulfate thermal springs have provided a better understanding of the microbial diversity of these communities and highlighted the importance of a number of metabolic processes, including oxygenic photosynthesis by the unicellular cyanobacterium Synechococcus lividus (31), cross-feeding between cyanobacteria and photoheterotrophic Chloroflexus-type filaments (2), nitrogen fixation (34), methanogenesis (40, 47), and acetogenesis, and the importance of anaerobic processes in decomposition and mineralization (1, 12, 34). Although the contribution of sulfate-reducing bacteria to processes in photosynthetic mats in high-sulfate hypersaline environments is well documented (11, 24, 25, 29, 30, 32), their contributions to processes in mats that develop in low-sulfate environments are less well studied. However, the study of sulfate respiration in microbial mats in low-sulfate environments is especially relevant to understanding the evolution of these ...
It is well known that electrostrictive strains are proportional to the square of the applied electric field. It therefore appeared reasonable to assume that for some polymeric materials, a large acoustic thickness response might be obtained by application of high dc bias fields, approx. 20 MV/rn, to a film while driving the film with an ac signal to access the high slope region of the electrostrictive strain vs. applied field curve. Previous studies of crystallizing poly(vinylidene fluoride) (PVF2) from solution under high electric fields have demonstrated that gel-like samples of PVF2 with high content of the plasticizer tricresyl phosphate (TCP) could he subjected to electric fields as high as approx. 100 MV/m. Using this type of heavily plasticized PVF2, d sub T values approx. 4 Angstrom/V were obtained. Values of 9 Angstrom/V were obtained for a certain class of thermoplastic elastomer (i.e., a polyurethane). These d sub T values are considerably greater than those obtained from conventional
Do you suffer from symptoms after eating? Such as digestive symptoms, foggy brain, sinus congestion or aches and pains. Do you notice it after gluten containing foods or just cant track whats causing it? Chances are youre body is reacting to components in plants called lectins.. What are lectins? So happy you asked!. Lectins are the plants natural compounds to ward off pests, fungal and bacterial attack. That is, the plants natural immune system. When plants are under attack they raise their lectin numbers to fend off the attacking pests. This is the system by which pesticides work. However, pests are gaining tolerance resulting in needing an increase of pesticide to be applied to the plant and thus a further increase of the plants lectins.. Modern wheat strains vs old style grains. Our modern wheat strains have been modified to produce more lectins to be highly pest resistant. This very clever genetically engineering won Norman Borlaug a Nobel peace prize in 1970 and changed the industry ...
145 Great Summary over here, 1. 146 Great Summary , 1. 147 See Sargons Tang-i Var DOWNLOAD CAMBRIDGE IGCSE CHEMISTRY WORKBOOK outBusiness; Frame 1999. cultural download science and civilisation in china. vol. 5, chemistry and chemical technology : pt. 11, ferrous metallurgy, Are Kahn 2001. 148 For the Ashdod download handbook of infrared astronomy, are Tadmor 1971. 149 Tadmor 1971: 192-195; Kapera 1976: 91-92. download ecophysiology of amphibians inhabiting xeric: anniversary provided Caroline Mulroney request Korean download? download ecophysiology of amphibians inhabiting convertible- together independently of his site? Ottawa: download laws Throne Speech asteroid to actors Find any of 440 journals from 1996s Royal Commission on Judaean Peoples which documents are 100 campaign clapped? Or exclusively seers from Harpers Truth and Reconciliation Commission? download ecophysiology of amphibians inhabiting xeric environments Catalina: access out the postcards & continue its combined database ...
Figure 9: Trace Analysis in End-Exhaled Air Using Direct Solvent Extraction in Gas Sampling Tubes: Tetrachloroethene in Workers as an Example
... The relationships between particle size of river sediments and the variation in stream discharge are studied. The results of a study carried out to determine the variation of both bed material and suspended sediment particle size for differing flow conditions is presented. A significant finding is presented which shows that the particle size of suspended and bed sediments remains relatively constant with variation in stream discharge. This condition was found from the analysis of results at four river sections on three rivers in Scotland and was also corroborated by results from research in the United States. Conclusions are presented which attempt to explain these findings in terms of the availability of material for transport by streams rather than on purely hydraulic considerations.
India Poly Vinyl Chloride (PVC) Market: Plant Capacity, Production, Operating Efficiency, Demand & Supply, End Use, Type, Grade, Distribution Channel, Region, Competition, ...
Vinyl chloride spilled from one of the train cars. NBC10s Tracy Davidson explains what the chemical is and why it can post a health hazard.
Buy Microbial Mats for $424.99 at Mighty Ape Australia. This book provides information about microbial mats, from early fossils to modern mats located in marine and terrestrial environments. Microbial mats ...
Trichloroethene (TCE) has become a chemical of interest after environmental activist Erin Brockovich suggested that the derailment of a train carrying chemicals 41 years ago could be involved in the mysterious illness striking 16 people, mostly high school students in New York.
Raw Material India (RMI) is a new startup - an online platform Only For Industrial Raw Materials of all types. This is Indias First-Of-Its-Kind Exclusive B2B Raw Materials website.. ...
Low-temperature anaerobic digestion (LTAD) presents a sustainable, cost-efficient technology for the treatment of a vast array of wastewater streams. However, this microbially-mediated process requires further understanding and experimental characterisation if large-scale application of LTAD is to be realised. The objective of this thesis was to employ a functional-based approach to characterise the microbial communities underpinning low-temperature anaerobic digestion. Firstly, the key microbial functional groups present in end-point samples taken from three, laboratory-scale, expanded granular sludge bed (EGSB) bioreactors; R1 (37°C), R2 (15°C) and R3 (7°C) were characterised. Metaproteomics, in conjunction with 16S rRNA gene phylogenetic approaches (clone libraries, qPCR), was applied to record microbial community composition and metaproteomic profiles as a function of bioreactor operating temperature. Clone libraries indicated a predominance of the Chloroflexi (21%) and ¿-Proteobacteria ...
The synthesis and degradation of polyhydroxyalkanoates (PHAs), the storage polymer of many bacteria, is linked to the operation of central carbon metabolism. To rationalize the impact of PHA accumulation on central carbon metabolism of the prototype bacterium Pseudomonas putida, we have revisited PHA production in quantitative physiology experiments in the wild-type strain vs. a PHA negative mutant growing under low nitrogen conditions. When octanoic acid was used as PHA precursor and as carbon and energy source, we have detected higher intracellular flux via acetyl-CoA in the mutant strain than in the wild type, which correlates with the stimulation of the TCA cycle and glyoxylate shunt observed on the transcriptional level. The mutant defective in carbon and energy storage spills the additional resources, releasing CO2 instead of generating biomass. Hence, P. putida operates the metabolic network to optimally exploit available resources and channels excess carbon and energy to storage via PHA, ...
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3-terminus.
Chloroflexi[edit]. Main article: Chloroflexi (phylum). Chloroflexi, a diverse phylum including thermophiles and halorespirers, ... Green non-sulfur bacteria and relatives (later renamed Chloroflexi[24]) *Chloroflexus group (Chloroflexus, Herpetosiphon) ... However, the candidate phylum TM7 may also be Gram positive.[16] Chloroflexi however possess a single bilayer, but stain ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ...
There are six classes within the phylum Chloroflexi: Chloroflexi, Anaerolinea, Caldilinea, Dehalococcoidia (previously known ... nov., within the phylum Chloroflexi" (PDF). Int. J. Syst. Evol. Microbiol. 63 (Pt 2): 625-635. doi:10.1099/ijs.0.034926-0. PMID ... Many of the species in Chloroflexi are thermophilic however Dehalogenimonas lykanthroporepellens is a mesophile.[7] The ... lykanthroporepellens also differ from other species in the phylum Chloroflexi in that they are not filamentous.[7] ...
Chloroflexi). The presence of this CSI in all sequenced species of conventional lipopolysaccharide-containing gram-negative ...
Nov., within the phylum Chloroflexi". International Journal of Systematic and Evolutionary Microbiology. 63 (Pt 2): 625-35. doi ...
The Chloroflexi have a single layer, yet (with some exceptions) stain negative. Two related phyla to the Chloroflexi, the TM7 ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ... Chloroflexi, etc.). The presence of this CSI in all sequenced species of conventional LPS (lipopolysaccharide)-containing Gram- ...
nov., within the phylum Chloroflexi". International Journal of Systematic and Evolutionary Microbiology. 63 (Pt 2): 625-635. ...
Chloroflexi), purple bacteria, Acidobacteria, and heliobacteria. The pigments used to carry out anaerobic photosynthesis are ...
Chloroflexi), the species stains Gram negative, yet has a single lipid layer (monoderm), but with thin peptidoglycan, which is ... Chloroflexi endosymbiotic theory Parte, A.C. "Chloroflexus". www.bacterio.net. Sutcliffe, I. C. (2010). "A phylum level ...
This trait is found in some proteobacteria, chloroflexi (green nonsulfur bacteria), low G+C gram positive Clostridia. and ... Reductive dechlorination Chloroflexi (phylum) Dehalococcoides Dehalobacter Holliger, C.; Wohlfarth, G.; Diekert, G. (1998). " ...
Chlorobiaceae and Chloroflexi) and Cyanobacteria. Purple and green bacteria oxidize sulfide, sulfur, sulfite, iron or hydrogen ...
Both families possess members from Firmicutes, Actinobacteria and Chloroflexi. Proteins of this superfamily all appear to have ...
... is a genus in the phylum Chloroflexi (Bacteria). The name Dehalogenimonas derives from: Latin prep. de, away, ...
One of the features of the members of the phylum Chloroflexi is the unusual cell wall structure, which is monoderm but with ... Dehalococcoides is a class of Chloroflexi, a phylum of Bacteria. It is also known as the DHC group. The name Dehalococcoidetes ... in the phylum Chloroflexi (emended description)". International Journal of Systematic and Evolutionary Microbiology. 54 (6): ...
... the male plurals Chloroflexi, Bacilli and Deinococci and the greek plurals Spirochaetes, Gemmatimonadetes and Chrysiogenetes).[ ...
The main types of microbe found there are Chloroflexi and Acidobacteria. They may be of astrobiological significance for Mars. ...
The main types of microbe found there are Chloroflexi and Acidobacteria. Mount Erebus was discovered on January 27, 1841 (and ...
... is one of two orders of bacteria in the class Chloroflexi. List of bacterial orders Garrity, George M. (2001). ...
2010 This section lists the orders of Bacteria within the phylum Chloroflexi. Anaerolineales Yamada et al. 2006 ...
Chloroflexi however possess a single bilayer, but stain negative (with some exceptions). Pasteuria is now assigned to phylum ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ... later renamed Chloroflexi) Chloroflexus group (Chloroflexus, Herpetosiphon) Thermomicrobium group (Thermomicrobium roseum) ...
... the Actinobacteria and Chloroflexi are monoderms. Candidate phylum TM7 is in fact a close relative of the Chloroflexi. Using a ...
They are also found in isolated species of Bacteroidetes, Chloroflexi and deltaproteobacteria. Several lines of evidence led to ...
Phototrophic bacteria are found in the phyla Cyanobacteria, Chlorobi, Proteobacteria, Chloroflexi, and Firmicutes. Along with ...
"Arsenite oxidase gene diversity among Chloroflexi and Proteobacteria from El Tatio Geyser Field, Chile". FEMS Microbiology ...
Filamentous anoxygenic phototrophs of the phylum Chloroflexi lack the FMO complex, but instead use a protein complex called ... Other Csm proteins with different letter suffixes can be found in Chloroflexi and Ca. Chloracidobacterium. Within the ...
... and Chloroflexi. All of these genera are found in petroleum reservoirs, and Sulfurimonas are present in high abundances. ...
Chloroflexi), the species stains Gram negative, yet has a single lipid layer (monoderm), but with thin peptidoglycan, which is ... Chloroflexi endosymbiotic theory Parte, A.C. "Chloroflexus". www.bacterio.net. Sutcliffe, I. C. (2010). "A phylum level ...
... green nonsulfur bacteria. Version 10 March 2006 (temporary). http://tolweb.org/Chloroflexi/2289/2006.03.10 in The ... in the phylum Chloroflexi (emended description). International Journal of Systematic and Evolutionary Microbiology 54(6):2049- ...
It has been suggested that the phylum Chloroflexi "sensu stricto" should comprise only the classes Chloroflexi and ... therefore currently the phylum Chloroflexi is divided into: Chloroflexi Gupta et al. 2012 Thermomicrobia Hugenholtz & ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes including members ... However, the classes "Chloroflexi" and Thermomicrobia were found to group together consistently by both the usual phylogenetic ...
PubMed references for Chloroflexi PubMed Central references for Chloroflexi Google Scholar references for Chloroflexi. ... The name "Chloroflexi" is a Neolatin plural of "Chloroflexus", which is the name of the first genus described. The noun is a ... Chloroflexi phy. nov". In D.R. Boone and R.W. Castenholz, eds. Bergeys Manual of Systematic Bacteriology Volume 1: The Archaea ... Classification of Chloroflexi entry in LPSN [Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a ...
The Chloroflexi-1 RNA motif is a conserved RNA structure detected by bioinformatics within the species Chloroflexus aggregans. ... C. aggregans has three predicted Chloroflexi-1 RNAs, which are located nearby to one another. This arrangement might suggest a ... C. aggregans is classified as belonging to the bacterial phylum Chloroflexi. Acido-Lenti-1 RNA motif Bacteroidales-1 RNA motif ...
It has been suggested that the phylum Chloroflexi sensu stricto should comprise only the classes Chloroflexi and Thermomicrobia ... The name Chloroflexi is a Neolatin nominative case masculine plural of Chloroflexus, which is the name of the first genus ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes, including ... Chloroflexi being a deep branching phylum (see Bacterial phyla), it was considered in Volume One of Bergeys Manual of ...
The phototrophic Chloroflexi carry out anoxygenic photosynthesis; they have a single type of photosystem and carry out cyclic ... General characteristics of the Chloroflexi. Diversity. Just over a dozen genera, with only one or sometimes two species in each ... Chloroflexus contains chlorosomes and bacteriochlorophyl c, whereas other Chloroflexi do not. These are traits otherwise found ... These chlorosomes resemble those of the Chlorobi (see below), but phototrophy in Chloroflexi otherwise resembles that of ...
Incubation with 13C-acetate and nanoSIMS (secondary ion mass-spectrometry) indicated higher uptake in both Chloroflexi and SRBs ... of Chloroflexi in GN-S mats (Ley et al., 2006) as well as a similar level of Chloroflexi pyrotags seen in GN-I mats. Moreover, ... Chloroflexi (Figure 1C), was divided between the phototrophic (class Chloroflexi) and the dark filamentous clades of the ... Chloroflexi (CFX1223 and GNSB-941) (A-D) and Desulfosarcina/Desulfobacteraceae (DSS658) hybridized filaments (E-H) shown in ...
Ang Chloroflexi ay isang uri ng phylum sa bakterya kahariang Protista. Hindi malaman kung saan ito maikakategorya, sa Gram- ... Kinuha mula sa "https://tl.wikipedia.org/w/index.php?title=Chloroflexi_(phylum)&oldid=1477440" ...
... ISME J ... we report on the discovery of a chemolithoautotrophic nitrite oxidizer that belongs to the widespread phylum Chloroflexi not ...
Cultivation of uncultured Chloroflexi subphyla: significance and ecophysiology of formerly uncultured Chloroflexi subphylum I ... It is likely that similar subsurface Chloroflexi have the potential for growth, since Chloroflexi sequences were recovered from ... Our nine Chloroflexi SAGs (seven of which are from the order Anaerolineales) indicate that, in addition to genes for the Wood- ... Nine Chloroflexi SSU rRNA gene sequences were recovered, four from hole C0015B and five from hole C0017C. Of the nine ...
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean. R. M. ... 1). Within the Chloroflexi phylum, the closest relatives to the SAR202 cluster could not be identified with the 16S rRNA gene ... Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean ... Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi. While members of this diverse group are ...
nov., within the phylum Chloroflexi. Int J Syst Evol Microbiol 63:625-635. doi:10.1099/ijs.0.034926-0. ... Cell envelope architecture in the Chloroflexi: a shifting frontline in a phylogenetic turf war. Environ Microbiol 13:279-282. ... Genome sequencing of a single cell of the widely distributed marine subsurface Dehalococcoidia, phylum Chloroflexi. ISME J 8: ... Detection of dsr genes in DEH bacteria indicates a potential for sulfite reduction in members of the phylum Chloroflexi and ...
Dechlorination of meta-Chlorinated Polychlorinated Biphenyl Congeners in Sediment Microcosms by Two Different Chloroflexi ... Dechlorination of meta-Chlorinated Polychlorinated Biphenyl Congeners in Sediment Microcosms by Two Different Chloroflexi ... Dechlorination of meta-Chlorinated Polychlorinated Biphenyl Congeners in Sediment Microcosms by Two Different Chloroflexi ... Dechlorination of meta-Chlorinated Polychlorinated Biphenyl Congeners in Sediment Microcosms by Two Different Chloroflexi ...
Chloroflexi abundance in HMA sponges.Recently, members of the phylum Chloroflexi were shown to be present in much higher ... Red clusters represent sponge-specific Chloroflexi cluster, whereas light gray clusters are built by nonsymbiotic Chloroflexi ... Central metabolism of sponge-associated Chloroflexi.Metabolic reconstruction suggests that Chloroflexi are aerobic and ... Cultivation of uncultured Chloroflexi subphyla: significance and ecophysiology of formerly uncultured Chloroflexi "subphylum I ...
f Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide ... Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide ...
strain MS-G (Chloroflexi). Authors: Schuster, Stephan Christoph. Bryant, Donald A.. Gay, Scott E.. Thiel, Vera. Hamilton, ... strain MS-G (Chloroflexi), isolated from Mushroom Spring (Yellowstone National Park, WY, USA) was sequenced and comprises ... strain MS-G (Chloroflexi). Genome Announcements, 2(5), e00872-14-. doi:10.1128/genomeA.00872-14. ...
Chloroflexi[edit]. Main article: Chloroflexi (phylum). Chloroflexi, a diverse phylum including thermophiles and halorespirers, ... Green non-sulfur bacteria and relatives (later renamed Chloroflexi[24]) *Chloroflexus group (Chloroflexus, Herpetosiphon) ... However, the candidate phylum TM7 may also be Gram positive.[16] Chloroflexi however possess a single bilayer, but stain ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ...
Chloroflexi.. The most dominant phylotype (Ced_B01) in the GPS1 belongs to the clade of candidate order MSBL5 in the phylum ... S2 and Table S1) (7). The lowest species richness was seen in GPS1, where the community was dominated by phyla Chloroflexi and ... 1 and Table S1). NS1 and BS5 also contained phyla of Chloroflexi and Firmicutes, but were dominated by a class ... In the spring fed solely by deep groundwater, phylum Chloroflexi, class Clostridia, and candidate division OD1 were the major ...
Draft Genome Sequence of a Divergent Anaerobic Member of the Chloroflexi Class Ardenticatenia from a Sulfidic Hot Spring L. M. ...
Test kit for rapid identification and quantification of the problem-causing Chloroflexi as well as Eikelboom Type 1851 ... VIT® Chloroflexi enables the fast and specific identification of the group of Chloroflexi filaments and of Eikelboom Type 1851 ... VIT® Chloroflexi. Test kit for rapid identification and quantification of filamentous bacteria. Product Number: 01110022 ... The Eikelboom Type 1851 for example is part of the Chloroflexi group and known as being problematic for sludge settling. ...
Chloroflexi[edit]. Bacteria in this phyla have a variety of extremophile traits. Some are moderate thermophiles, aerobic, ... These bacteria are extremophiles that consist of the phyla: Aquificae, Thermotogae, Thermus-Deinococcus, and Chloroflexi. These ...
It has been suggested that the phylum Chloroflexi "sensu stricto" should comprise only the classes Chloroflexi and ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes including members ... "Chloroflexi". List of Prokaryotic names with Standing in Nomenclature (LPSN) [1]. Retrieved 2014-03-20. ... "Chloroflexi". National Center for Biotechnology Information (NCBI) taxonomy database [2]. Retrieved 2014-03-20. ...
Chloroflexi. (redirected from Chlorobacteria) Chloroflexi. A prokaryote genus name with no current standing in prokaryotic ...
Chloroflexi have been implicated in BNR systems by phylogenetic identification of filamentous bacteria isolated by ... Specific 16S rRNA-targeted oligonucleotide probes were designed for the phylum Chloroflexi (green non-sulfur bacteria) and ... Filamentous bacteria responding to a subdivision 1 Chloroflexi probe were rare in the samples, whereas subdivision 3 ... It is suggested that filamentous bacteria belonging to the Chloroflexi phylum account for a large fraction of phylogenetically ...
  • Chloroflexi), the species stains Gram negative, yet has a single lipid layer (monoderm), but with thin peptidoglycan, which is compensated for by S-layer protein. (wikipedia.org)
  • In 1987, Carl Woese, regarded as the forerunner of the molecular phylogeny revolution, divided Eubacteria into 11 divisions based on 16S ribosomal RNA (SSU) sequences and grouped the genera Chloroflexus, Herpetosiphon and Thermomicrobium into the "Green non-sulfur bacteria and relatives", which was temporarily renamed as "Chloroflexi" in Volume One of Bergey's Manual of Systematic Bacteriology. (wikipedia.org)
  • The name "Chloroflexi" is a Neolatin plural of "Chloroflexus", which is the name of the first genus described. (wikipedia.org)
  • The Chloroflexi-1 RNA motif is a conserved RNA structure detected by bioinformatics within the species Chloroflexus aggregans. (wikipedia.org)
  • Chloroflexus contains chlorosomes and bacteriochlorophyl c , whereas other Chloroflexi do not. (ncsu.edu)
  • In these communities, it is the cyanobacteria that are the primary producers, and the Chloroflexi grow photoheterotrophically from organic carbon produced by the cyanobacteria. (ncsu.edu)
  • These manipulations and diel incubations confirm that Cyanobacteria were the main fermenters in Guerrero Negro mats and that the net flux of nighttime fermentation byproducts (not only hydrogen) was largely regulated by the interplay between Cyanobacteria , SRBs, and Chloroflexi . (frontiersin.org)
  • In comparisons among Chloroflexi -containing sites that were rich in methane hydrates and organic carbon off the Peru and Cascade Margins, Chloroflexi SSU rRNA gene sequences were more numerous at the organic-rich sites ( 8 ). (asm.org)
  • Based on their SSU rRNA gene identities, nine Chloroflexi SAGs (of the total 29 unique MDA reactions identified after cell sorting) were chosen for whole-genome sequencing. (bco-dmo.org)
  • Using a single-cell genomics approach, we obtained the first draft genomic reconstruction for these organisms and compared their inferred metabolic potential with free-living chloroflexi. (uni-bielefeld.de)
  • Diversity and genomic insights into the uncultured Chloroflexi from the human microbiota", Environmental Microbiology , vol. 16, 2014, pp. 2635-2643. (uni-bielefeld.de)
  • It is likely that similar subsurface Chloroflexi have the potential for growth, since Chloroflexi sequences were recovered from an in situ colonization experiment within a borehole, indicating recent growth on previously uninhabited surfaces ( 13 ). (asm.org)
  • In the Sponge Microbiome Project, Chloroflexi sequences amounted to 20 to 30% of the total microbiome of certain HMA sponge genera with the classes/clades SAR202, Caldilineae , and Anaerolineae being the most prominent. (asm.org)
  • Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removalccThe EMBL accession numbers for the sequences reported in this paper are X84472 (strain SBR1029 16S rDNA), X84474 (strain SBR1031 16S rDNA), X84498 (strain SBR1064 16S rDNA), X84565 (strain SBR2022 16S rDNA), X84576 (strain SBR2037 16S rDNA) and X84607 (strain SBR2076 16S rDNA). (microbiologyresearch.org)
  • A distinct group of uncultured chloroflexi related to free-living anaerobic Anaerolineae inhabits the mammalian gastrointestinal tract and includes low-abundance human oral bacteria that appear to proliferate in periodontitis. (uni-bielefeld.de)
  • Genomic data suggest that oral chloroflexi are anaerobic heterotrophs, encoding abundant carbohydrate transport and metabolism functionalities, similar to those seen in environmental Anaerolineae isolates. (uni-bielefeld.de)
  • Previous studies of subsurface Chloroflexi single amplified genomes (SAGs) suggested heterotrophic or lithotrophic metabolisms and provided no evidence for growth by reductive dehalogenation. (asm.org)
  • Our nine Chloroflexi SAGs (seven of which are from the order Anaerolineales ) indicate that, in addition to genes for the Wood-Ljungdahl pathway, exogenous carbon sources can be actively transported into cells. (asm.org)
  • At least one subunit for pyruvate ferredoxin oxidoreductase was found in four of the Chloroflexi SAGs. (asm.org)
  • In contrast to previously examined Chloroflexi SAGs, our genomes (several are from the order Anaerolineales ) were recovered from a hydrothermally driven system and therefore provide a unique window into the metabolic potential of this type of habitat. (asm.org)
  • Chloroflexi consists of the green non-sulfur bacteria which are anoxygenic phototrophs (do not produce oxygen during photosynthesis ) that use either H 2 or H 2 S as an electron donor . (wikipedia.org)
  • Chloroflexi are the deepest branching (oldest) anoxygenic phototrophs on the tree of life . (wikipedia.org)
  • Other Csm proteins with different letter suffixes can be found in Chloroflexi and Ca. Chloracidobacterium. (wikipedia.org)
  • These chlorosomes resemble those of the Chlorobi (see below), but phototrophy in Chloroflexi otherwise resembles that of typical purple bacterial photosynthesis. (ncsu.edu)
  • Most of the Chloroflexi filaments are uncultivable and unknown, so that knowledge about them is limited. (vermicon.com)
  • VIT® Chloroflexi enables the fast and specific identification of the group of Chloroflexi filaments and of Eikelboom Type 1851 in sludge samples. (vermicon.com)
  • The distinctive gene content of this cell suggests metabolic characteristics that differ from those of known DEH and Chloroflexi . (asm.org)
  • We used metagenomic and single-cell genomic approaches to characterize the functional gene repertoire of Chloroflexi symbionts in marine sponges. (asm.org)
  • Among them, the Chloroflexi comprises taxonomically and physiologically diverse lineages adapted to a wide range of aquatic and terrestrial habitats. (uni-bielefeld.de)
  • Common genomic features of Chloroflexi sponge symbionts were related to central energy and carbon converting pathways, amino acid and fatty acid metabolism, and respiration. (asm.org)
  • The presence of genes for a unique phosphotransferase system and N-acetylglucosamine metabolism suggests an important ecological niche for oral chloroflexi in scavenging material from lysed bacterial cells and the human tissue. (uni-bielefeld.de)
  • A metagenomic study of the Peru Margin showed Chloroflexi to be present at a broad range of depths and to compose 12 to 16% of the total genes identified ( 9 ). (asm.org)
  • The presence of genes encoding dissimilatory sulfite reductase (Dsr) suggests that DEH could respire oxidized sulfur compounds, although Chloroflexi have never been implicated in this mode of sulfur cycling. (asm.org)
  • As with other low abundance but persistent members of the microbiota, discerning community and host factors that influence the proliferation of oral chloroflexi may help understand the emergence of oral pathogens and the microbiota dynamics in health and disease states. (uni-bielefeld.de)
  • strain MS-G (Chloroflexi), isolated from Mushroom Spring (Yellowstone National Park, WY, USA) was sequenced and comprises 4,784,183 bp in 251 contigs. (ntu.edu.sg)