Phylum of green nonsulfur bacteria including the family Chloroflexaceae, among others.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The relationships of groups of organisms as reflected by their genetic makeup.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Class of BACTERIA with diverse morphological properties. Strains of Actinobacteria show greater than 80% 16S rDNA/rRNA sequence similarity among each other and also the presence of certain signature nucleotides. (Stackebrandt E. et al, Int. J. Syst. Bacteriol. (1997) 47:479-491)
Refuse liquid or waste matter carried off by sewers.
Hot springs on the ocean floor. They are commonly found near volcanically active places such as mid-oceanic ridges.
A genus of green nonsulfur bacteria in the family Chloroflexaceae. They are photosynthetic, thermophilic, filamentous gliding bacteria found in hot springs.
A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.
Disposal, processing, controlling, recycling, and reusing the solid, liquid, and gaseous wastes of plants, animals, humans, and other organisms. It includes control within a closed ecological system to maintain a habitable environment.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
The variety of all native living organisms and their various forms and interrelationships.
Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Habitat of hot water naturally heated by underlying geologic processes. Surface hot springs have been used for BALNEOLOGY. Underwater hot springs are called HYDROTHERMAL VENTS.
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
A group of different species of microorganisms that act together as a community.
A collective genome representative of the many organisms, primarily microorganisms, existing in a community.
Deoxyribonucleic acid that makes up the genetic material of archaea.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Measurable quantity of bacteria in an object, organism, or organism compartment.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)
The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A chlorinated hydrocarbon used as an industrial solvent and cooling liquid in electrical transformers. It is a potential carcinogen.
A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.
2-Hydroxy-N-phenylbenzamides. N-phenyl substituted salicylamides. Derivatives have been used as fungicides, anti-mildew agents and topical antifungal agents. In concentrated form may cause irritation of skin and mucous membranes.
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.
The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.
Contaminated water generated as a waste product of human activity.
Worthless, damaged, defective, superfluous or effluent material from industrial operations.
Individual's rights to obtain and use information collected or generated by others.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
A publication issued at stated, more or less regular, intervals.
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
Liquid water present beneath the surface of the earth.
Phenols substituted with one or more chlorine atoms in any position.

Bacterial diversity and sulfur cycling in a mesophilic sulfide-rich spring. (1/121)

An artesian sulfide- and sulfur-rich spring in southwestern Oklahoma is shown to sustain an extremely rich and diverse microbial community. Laboratory incubations and autoradiography studies indicated that active sulfur cycling is occurring in the abundant microbial mats at Zodletone spring. Anoxygenic phototrophic bacteria oxidize sulfide to sulfate, which is reduced by sulfate-reducing bacterial populations. The microbial community at Zodletone spring was analyzed by cloning and sequencing 16S rRNA genes. A large fraction (83%) of the microbial mat clones belong to sulfur- and sulfate-reducing lineages within delta-Proteobacteria, purple sulfur gamma-Proteobacteria, epsilon -Proteobacteria, Chloroflexi, and filamentous Cyanobacteria of the order Oscillatoria as well as a novel group within gamma-Proteobacteria. The 16S clone library constructed from hydrocarbon-exposed sediments at the source of the spring had a higher diversity than the mat clone library (Shannon-Weiner index of 3.84 compared to 2.95 for the mat), with a higher percentage of clones belonging to nonphototrophic lineages (e.g., Cytophaga, Spirochaetes, Planctomycetes, Firmicutes, and Verrucomicrobiae). Many of these clones were closely related to clones retrieved from hydrocarbon-contaminated environments and anaerobic hydrocarbon-degrading enrichments. In addition, 18 of the source clones did not cluster with any of the previously described microbial divisions. These 18 clones, together with previously published or database-deposited related sequences retrieved from a wide variety of environments, could be clustered into at least four novel candidate divisions. The sulfate-reducing community at Zodletone spring was characterized by cloning and sequencing a 1.9-kb fragment of the dissimilatory sulfite reductase (DSR) gene. DSR clones belonged to the Desulfococcus-Desulfosarcina-Desulfonema group, Desulfobacter group, and Desulfovibrio group as well as to a deeply branched group in the DSR tree with no representatives from cultures. Overall, this work expands the division-level diversity of the bacterial domain and highlights the complexity of microbial communities involved in sulfur cycling in mesophilic microbial mats.  (+info)

Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. (2/121)

Two thermophilic, Gram-negative, non-spore-forming, multicellular filamentous micro-organisms were isolated from thermophilic granular sludge in an upflow anaerobic sludge blanket reactor treating fried soybean-curd manufacturing waste water (strain UNI-1(T)) and from a hot spring sulfur-turf in Japan (strain STL-6-O1(T)). The filaments were longer than 100 microm and of 0.2-0.3 microm (strain UNI-1(T)) or 0.7-0.8 microm (strain STL-6-O1(T)) in width. Strain UNI-1(T) was a strictly anaerobic organism. The optimum temperature for growth was around 55 degrees C; growth occurred in the range 50-60 degrees C. The optimum pH for growth was around 7.0; growth occurred in the range pH 6.0-8.0. Strain STL-6-O1(T) was a facultatively aerobic bacterium. The optimum temperature for growth was around 55 degrees C; growth occurred in the range 37-65 degrees C. The optimum pH for growth was around 7.5-8.0; growth occurred in the range pH 7.0-9.0. The two organisms grew chemo-organotrophically on a number of carbohydrates and amino acids in the presence of yeast extract. The G+C content of the DNA of strains UNI-1(T) and STL-6-O1(T) was 54.5 and 59.0 mol%, respectively. Major cellular fatty acids for strain UNI-1(T) were C(16 : 0), C(15 : 0), C(14 : 0) and C(18 : 0), whereas those for strain STL-6-O1(T) were C(18 : 0), C(16 : 0), C(17 : 0) and iso-C(17 : 0). MK-10 was the major quinone from aerobically grown STL-6-O1(T) cells. Phylogenetic analyses based on 16S rDNA sequences revealed that both strains belong to an uncultured, previously recognized clone lineage of the phylum Chloroflexi (formerly known as green non-sulfur bacteria). These phenotypic and genetic properties suggested that each strain should be classified into a new independent genus; hence, the names Anaerolinea thermophila and Caldilinea aerophila are proposed for strains UNI-1(T) (=JCM 11387(T)=DSM 14523(T)) and STL-6-O1(T)(=JCM 11388(T)=DSM 14525(T)), respectively. These strains represent the type and sole species of the genera Anaerolinea and Caldilinea, respectively.  (+info)

Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean. (3/121)

Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi: While members of this diverse group are consistently identified in the marine environment, there are currently no cultured representatives, and very little is known about their distribution or abundance in the world's oceans. In this study, published and newly identified SAR202-related 16S rRNA gene sequences were used to further resolve the phylogeny of this cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. Direct cell counts from the Bermuda Atlantic time series study site in the north Atlantic Ocean, the Hawaii ocean time series site in the central Pacific Ocean, and along the Newport hydroline in eastern Pacific coastal waters showed that SAR202 cluster cells were most abundant below the deep chlorophyll maximum and that they persisted to 3600 m in the Atlantic Ocean and to 4000 m in the Pacific Ocean, the deepest samples used in this study. On average, members of the SAR202 group accounted for 10.2% (+/-5.7%) of all DNA-containing bacterioplankton between 500 and 4000 m.  (+info)

A gene from the mesophilic bacterium Dehalococcoides ethenogenes encodes a novel mannosylglycerate synthase. (4/121)

Mannosylglycerate (MG) is a common compatible solute found in thermophilic and hyperthermophilic prokaryotes. In this study we characterized a mesophilic and bifunctional mannosylglycerate synthase (MGSD) encoded in the genome of the bacterium Dehalococcoides ethenogenes. mgsD encodes two domains with extensive homology to mannosyl-3-phosphoglycerate synthase (MPGS, EC 2.4.1.217) and to mannosyl-3-phosphoglycerate phosphatase (MPGP, EC 3.1.3.70), which catalyze the consecutive synthesis and dephosphorylation of mannosyl-3-phosphoglycerate to yield MG in Pyrococcus horikoshii, Thermus thermophilus, and Rhodothermus marinus. The bifunctional MGSD was overproduced in Escherichia coli, and we confirmed the combined MPGS and MPGP activities of the recombinant enzyme. The optimum activity of the enzyme was at 50 degrees C. To examine the properties of each catalytic domain of MGSD, we expressed them separately in E. coli. The monofunctional MPGS was unstable, while the MPGP was stable and was characterized. Dehalococcoides ethenogenes cannot be grown sufficiently to identify intracellular compatible solutes, and E. coli harboring MGSD did not accumulate MG. However, Saccharomyces cerevisiae expressing mgsD accumulated MG, confirming that this gene product can synthesize this compatible solute and arguing for a role in osmotic adjustment in the natural host. We did not detect MGSD activity in cell extracts of S. cerevisiae. Here we describe the first gene and enzyme for the synthesis of MG from a mesophilic microorganism and discuss the possible evolution of this bifunctional MGSD by lateral gene transfer from thermophilic and hyperthermophilic organisms.  (+info)

Populations implicated in anaerobic reductive dechlorination of 1,2-dichloropropane in highly enriched bacterial communities. (5/121)

1,2-Dichloropropane (1,2-D), a widespread groundwater contaminant, can be reductively dechlorinated to propene by anaerobic bacteria. To shed light on the populations involved in the detoxification process, a comprehensive 16S rRNA gene-based bacterial community analysis of two enrichment cultures derived from geographically distinct locations was performed. Analysis of terminal restriction fragments, amplicons obtained with dechlorinator-specific PCR primers, and enumeration with quantitative real-time PCR as well as screening clone libraries all implied that Dehalococcoides populations were involved in 1,2-D dechlorination in both enrichment cultures. Physiological traits (e.g., dechlorination in the presence of ampicillin and a requirement for hydrogen as the electron donor) supported the involvement of Dehalococcoides populations in the dechlorination process. These findings expand the spectrum of chloroorganic compounds used by Dehalococcoides species as growth-supporting electron acceptors. The combined molecular approach allowed a comparison between different 16S rRNA gene-based approaches for the detection of Dehalococcoides populations.  (+info)

Recommended standards for the description of new species of anoxygenic phototrophic bacteria. (6/121)

Recommended standards for the description of new species of the anoxygenic phototrophic bacteria are proposed in accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria. These standards include information on the natural habitat, ecology and phenotypic properties including morphology, physiology and pigments and on genetic information and nucleic acid data. The recommended standards were supported by the Subcommittee on the taxonomy of phototrophic bacteria of the International Committee on Systematics of Prokaryotes. They are considered as guidelines for authors to prepare descriptions of new species.  (+info)

Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. (7/121)

Reductive dehalogenation of vinyl chloride (VC) to ethene is the key step in complete anaerobic degradation of chlorinated ethenes. VC-reductive dehalogenase was partially purified from a highly enriched culture of the VC-respiring Dehalococcoides sp. strain VS. The enzyme reduced VC and all dichloroethene (DCE) isomers, but not tetrachloroethene (PCE) or trichloroethene (TCE), at high rates. By using reversed genetics, the corresponding gene (vcrA) was isolated and characterized. Based on the predicted amino acid sequence, VC reductase is a novel member of the family of corrinoid/iron-sulfur cluster containing reductive dehalogenases. The vcrA gene was found to be cotranscribed with vcrB, encoding a small hydrophobic protein presumably acting as membrane anchor for VC reductase, and vcrC, encoding a protein with similarity to transcriptional regulators of the NosR/NirI family. The vcrAB genes were subsequently found to be present and expressed in other cultures containing VC-respiring Dehalococcoides organisms and could be detected in water samples from a field site contaminated with chlorinated ethenes. Therefore, the vcrA gene identified here may be a useful molecular target for evaluating, predicting, and monitoring in situ reductive VC dehalogenation.  (+info)

Dominant microbial composition and its vertical distribution in saline meromictic Lake Kaiike (Japan) as revealed by quantitative oligonucleotide probe membrane hybridization. (8/121)

Vertical distributions of dominant bacterial populations in saline meromictic Lake Kaiike were investigated throughout the water column and sediment by quantitative oligonucleotide probe membrane hybridization. Three oligonucleotide probes specific for the small-subunit (SSU) rRNA of three groups of Chlorobiaceae were newly designed. In addition, three general domain (Bacteria, Archaea, and Eukarya)-specific probes, two delta-Proteobacteria-specific probes, a Chlorobiaceae-specific probe, and a Chloroflexi-specific probe were used after optimization of their washing conditions. The abundance of the sum of SSU rRNAs hybridizing with probes specific for three groups of Chlorobiaceae relative to total SSU rRNA peaked in the chemocline, accounting for up to 68%. The abundance of the delta-proteobacterial SSU rRNA relative to total SSU rRNA rapidly increased just below the chemocline up to 29% in anoxic water and peaked at the 2- to 3-cm sediment depth at ca. 34%. The abundance of SSU rRNAs hybridizing with the probe specific for the phylum Chloroflexi relative to total SSU rRNA was highest (31 to 54%) in the top of the sediment but then steeply declined with depth and became stable at 11 to 19%, indicating the robust coexistence of sulfate-reducing bacteria and Chloroflexi in the top of the sediment. Any SSU rRNA of Chloroflexi in the water column was under the detection limit. The summation of the signals of group-specific probes used in this study accounted for up to 89% of total SSU rRNA, suggesting that the DGGE-oligonucleotide probe hybridization approach, in contrast to conventional culture-dependent approaches, was very effective in covering dominant populations.  (+info)

Author(s): Zhuang, Wei-Qin; Yi, Shan; Bill, Markus; Brisson, Vanessa L; Feng, Xueyang; Men, Yujie; Conrad, Mark E; Tang, Yinjie J; Alvarez-Cohen, Lisa | Abstract: The acetyl-CoA Wood-Ljungdahl pathway couples the folate-mediated one-carbon (C1) metabolism to either CO2 reduction or acetate oxidation via acetyl-CoA. This pathway is distributed in diverse anaerobes and is used for both energy conservation and assimilation of C1 compounds. Genome annotations for all sequenced strains of Dehalococcoides mccartyi, an important bacterium involved in the bioremediation of chlorinated solvents, reveal homologous genes encoding an incomplete Wood-Ljungdahl pathway. Because this pathway lacks key enzymes for both C1 metabolism and CO2 reduction, its cellular functions remain elusive. Here we used D. mccartyi strain 195 as a model organism to investigate the metabolic function of this pathway and its impacts on the growth of strain 195. Surprisingly, this pathway cleaves acetyl-CoA to donate a methyl group for
Different Dehalococcoides strains contain different numbers of rdh genes that encode protein, which have been proven or predicted to catalyse the dechlorination reaction. When compared with the genomes of other dechlorinating bacteria, Dehalococcoides have the highest number of rdh genes in their genomes (Table 3). Genomes of strains 195, CBDB1 and BAV1 have 17, 32 and 10 rdh genes, respectively, whereas only seven rdh genes were identified in the genome of Desulfitobacterium hafniense DCB-2, four rdh genes in D. hafniense Y51 and two rdh genes in Geobacter lovleyi SZ and Anaeromyxobacter dehalogenans (Thomas et al., 2008). The draft genome of strain VS contains the highest number of rdh genes (36 full-length genes) ever found in a single bacterial genome (McMurdie et al., 2008). Similarly, 14 and 19 rdh genes were detected via PCR amplification in Dehalococcoides sp. strains FL2 and DCMB5 respectively (Holscher et al., 2004; Bunge et al., 2008). Twelve rdh genes from strain CBDB1 have ...
Reductive dehalogenases are the critical enzymes for anaerobic organohalide respiration, a microbial metabolic process that has been harnessed for bioremediation efforts to resolve chlorinated solvent contamination in groundwater and is implicated in the global halogen cycle. Reductive dehalogenase sequence diversity is informative for the dechlorination potential of the site or enrichment culture. A suite of degenerate PCR primers targeting a comprehensive curated set of reductive dehalogenase genes was designed and applied to twelve DNA samples extracted from contaminated and pristine sites, as well as six enrichment cultures capable of reducing chlorinated compounds to non-toxic end-products. The amplified gene products from four environmental sites and two enrichment cultures were sequenced using Illumina HiSeq, and the reductive dehalogenase complement of each sample determined. The results indicate that the diversity of the reductive dehalogenase gene family is much deeper than is currently
TY - JOUR. T1 - Seasonal dominance of CL500-11 bacterioplankton (phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan. AU - Okazaki, Yusuke. AU - Hodoki, Yoshikuni. AU - Nakano, Shin Ichi. PY - 2013/1/1. Y1 - 2013/1/1. N2 - Uncultured bacteria affiliated with the CL500-11 cluster (phylum Chloroflexi) were first reported from the oxygenated hypolimnion of Crater Lake (USA) as a predominant bacterioplankton, although this dominance has not been reported in other environments. In this study, we showed that CL500-11 is also dominant in the oxygenated hypolimnion of Lake Biwa (Japan) and followed its spatiotemporal succession using fluorescent in situ hybridization. CL500-11 cells were almost absent [, 1% of 4′,6-diamidino-2-phenylindole (DAPI)-stained cells] at the beginning of the stratification period, dominated (, 10% of DAPI-stained cells; maximum = 16.5%) in the hypolimnion during the stratification period, and decreased to below the detection limit with the collapse of the ...
Regarding the class Dehalococcoidetes, the placeholder name was given by Hugenholtz & Stackebrandt, 2004,[6] after Dehalococcoides ethenogenes a partially described species in 1997,[7] whereas the first species fully described was Dehalogenimonas lykanthroporepellens by Moe et al. 2009,[8] but in the description of that species the class was not made official nor were families or orders laid out as the two species share only 90% 16S identity, meaning that they could fall in different families or even orders.[8] Recent phylogenetic analysis of the Chloroflexi has found very weak support for the grouping together of the different classes currently part of the phylum.[9] The six classes that make up the phylum did not consistently form a well-supported monophyletic clade in phylogenetic trees based on concatenated sequences for large datasets of proteins and no conserved signature indels were identified that were uniquely shared by the entire phylum.[9] However, the classes Chloroflexi and ...
Dehalococcoidetes is a placeholder name given by Hugenholtz & Stackebrandt, 2004,[7] after Dehalococcoides ethenogenes a species partially described in 1997.[8] The first species fully described was Dehalogenimonas lykanthroporepellens, by Moe et al. 2009,[9] but in the description of that species the class was not made official nor were families or orders laid out as the two species share only 90% 16S ribosomal RNA identity, meaning that they could fall in different families or even orders.[9]. Recent phylogenetic analysis of the Chloroflexi has found very weak support for the grouping together of the different classes currently part of the phylum.[10] The six classes that make up the phylum did not consistently form a well-supported clade in phylogenetic trees based on concatenated sequences for large datasets of proteins, and no conserved signature indels were identified that were uniquely shared by the entire phylum.[10] However, the classes Chloroflexi and Thermomicrobia were found to ...
This study explored the potential of eutrophic river sediments to attenuate the infiltration of chlorinated aliphatic hydrocarbon (CAH)-polluted groundwater discharging into the Zenne River near Brussels, Belgium. Active biotic reductive dechlorination of CAHs in the riverbed was suggested by a high dechlorination activity in batch- and column biodegradation tests performed with sediment samples, and by the detection of dechlorination products in sediment pore water. Halorespiring Dehalococcoides spp. were present in large numbers in the riverbed as shown by quantification of their 16S rRNA and reductive dehalogenase genes. By using DGGE-fingerprint analysis of relevant nucleic acid markers, it was shown that the Zenne River sediments were inhabited by a metabolically diverse bacterial community. A large diversity of sulfate-reducing bacteria, Geobacteraceae and methanogens, which potentially compete with halorespiring bacteria for electron resources, was identified. The high organic carbon ...
This study explored the potential of eutrophic river sediments to attenuate the infiltration of chlorinated aliphatic hydrocarbon (CAH)-polluted groundwater discharging into the Zenne River near Brussels, Belgium. Active biotic reductive dechlorination of CAHs in the riverbed was suggested by a high dechlorination activity in batch- and column biodegradation tests performed with sediment samples, and by the detection of dechlorination products in sediment pore water. Halorespiring Dehalococcoides spp. were present in large numbers in the riverbed as shown by quantification of their 16S rRNA and reductive dehalogenase genes. By using DGGE-fingerprint analysis of relevant nucleic acid markers, it was shown that the Zenne River sediments were inhabited by a metabolically diverse bacterial community. A large diversity of sulfate-reducing bacteria, Geobacteraceae and methanogens, which potentially compete with halorespiring bacteria for electron resources, was identified. The high organic carbon ...
Enrichment cultures are investigated for the bioremediation potential of tetrachloroethene (PCE). This process is based on anaerobic respiration in which chlorinated compounds are used as electron acceptors (organohalide respiration, OHR). The key enzyme in OHR is the reductive dehalogenase (RdhA). SL2, a culture dechlorinating PCE to harmless ethene, was shown to have a small number of OHR bacteria, mainly Sulfurospirillum spp. and Dehalococcoides spp. (1). Aim: We aim at a better understanding of the functional diversity of rdhA genes in the SL2 culture and their dynamic during PCE degradation. Methods: By applying different frequencies of culture transfer, 3 sub-cultures were obtained from the SL2 enrichment showing different degradation patterns. The degradation profile was followed by gas chromatography and chloride titration. DNA and RNA were also extracted, PCR and qPCR was performed for the detection of rdhA genes belonging to Sulfurospirillum. Besides, a T-RFLP method was developed to ...
Molecular data show that the filamentous bacterium Eikelboom type 0092, frequently seen in Australian activated sludge plants, is a member of the phylum Chloroflexi. Fluorescence in situ hybridization (FISH) probes designed against cloned 16S rRNA sequences from a full-scale enhanced biological phosphate removal-activated sludge plant community, where this was a dominant filament morphotype, suggest that it can exist as two variants, differing in their trichome diameter. When applied to samples from several treatment plants in eastern Australia, each FISH probe targeted only the type 0092 filament morphotype against which it was designed. The patterns of FISH signals generated with both were consistent with the ribosomes not being evenly distributed but arranged as intracellular aggregates. The FISH survey data showed that these two variants appeared together in most but not all of the plants examined. None stained positively for intracellular presence of either poly-beta-hydroxyalkanoates or ...
Polychlorinated aliphatic C2 and C3 alkanes (ethanes and propanes with at least two chlorine substituents) are industrially important chemical intermediates globally produced on a massive scale.[11] Due to spills and past inappropriate disposal methods, these chlorinated compounds are prevalent groundwater and soil contaminants throughout the US and around the world.[11] Bioremediation approaches that rely on the action of anaerobic, reductively-dehalogenating bacteria, such as D. lykanthroporepellens, have shown great promise for clean-up of chlorinated solvent-contaminated soil and groundwater.[4] Using qPCR (quantitative real-time polymerase chain reaction), 16S rRNA gene sequences for Dehalogenimonas strains have been found to be at concentrations as high as 106 copies/ml of groundwater contaminated with high concentrations of chlorinated solvents and comprise up to nearly 19% of the total bacterial 16S rRNA gene copies.[12] The characterization of D. lykanthroporepellens has aided in ...
Two recently reported bacterial strains that are able to reductively dehalogenate polychlorinated aliphatic alkanes, including 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2-dichloroethane, were further characterized to clarify their taxonomic position. The two strains, designated BL-DC-8 and BL-DC-9T, were mesophilic, non-spore-forming, non-motile, Gram-negative staining and strictly anaerobic. Cells were irregular cocci, 0.3-0.6 μm in diameter. The two strains were resistant to ampicillin and vancomycin. Hydrogen was utilized as an electron donor. The genomic DNA G+C content of strains BL-DC-8 and BL-DC-9T was 54.0 and 53.8 mol%, respectively. The major cellular fatty acids were C18 : 1 ω9c, C16 : 1 ω9c, C16 : 0 and C14 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the strains cluster within the phylum Chloroflexi, but are related only distantly to all recognized taxa in the phylum. Morphological, physiological and
SiREM offers a growing number of targets for Gene-Trac® testing that includes: Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg), Desulfitobacterium (Dsb) and key functional genes such as vinyl chloride reductase (vcrA, bvcA), trichloroethene reductase (tceA), chloroform, 1,1,1-TCA and 1,1-DCA reductase (cfrA/dcrA). These microorganisms and functional genes are critical to reductive dechlorination of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes, and propanes. Geobacter and sulfate reducers play key roles in the metabolism of aromatic hydrocarbon compounds and the generation of iron sulfur compounds critical to natural attenuation remedies. The dxmB and ALDH genes are important in the aerobic degradation of 1,4-dioxane.. Gene-Trac® quantitative polymerase chain reaction (qPCR) testing is used to quantify key dechlorinating bacteria and functional genes. If a comprehensive analysis of microbial community is required Gene-Trac® NGS (next ...
The discovery of Dehalococcoides mccartyi reducing perchloroethene and trichloroethene (TCE) to ethene was a key landmark for bioremediation applications at contaminated sites. D. mccartyi-containing cultures are typically grown in batch-fed reactors. On the other hand, continuous cultivation of these microorganisms has been described only at long hydraulic retention times (HRTs). We report the cultivation of a representative D. mccartyi-containing culture in continuous stirred-tank reactors (CSTRs) at a short, 3-d HRT, using TCE as the electron acceptor. We successfully operated 3-d HRT CSTRs for up to 120 days and observed sustained dechlorination of TCE at influent concentrations of 1 and 2 mM TCE to ≥97 % ethene, coupled to the production of 1012 D. mccartyi cells Lculture −1. These outcomes were possible in part by using a medium with low bicarbonate concentrations (5 mM) to minimize the excessive proliferation of microorganisms that use bicarbonate as an electron acceptor and compete with D.
Photosynthetic organisms have evolved diverse antennas to harvest light of various qualities and intensities. Anoxygenic phototrophs can have bacteriochlorophyll Qy antenna absorption bands ranging from about 700-1100 nm. This broad range of usable wavelengths has allowed many organisms to thrive in unique environments. Roseiflexus castenholzii is a niche-adapted, filamentous anoxygenic phototroph: FAP) that lacks chlorosomes, the dominant antenna found in all green bacteria. Light-harvesting is realized only in the membrane with BChl a and a variety of carotenoids. Through biochemical and spectroscopic methods, a model for the size and organization of the photosynthetic antenna is presented. Despite the wide distribution of antennas, photochemistry occurs in the reaction center: RC), which can be separated into two groups distinguishable by the identity of the terminal electron acceptor. These are the Fe-S type or type-I and the quinone-type or type -II RCs. All known anoxygenic phototrophs have
氯乙烯類化合物廣泛應用於乾洗及金屬除油,但因不當處置或意外滲漏,使氯乙烯類化合物污染土壤與地下水體,又因氯乙烯類污染物具高毒性,對環境與民眾健康構成嚴重威脅。基於環境友善與經濟效益,生物復育是目前推崇的整治方法之一,但採用生物復育策略之前,須對污染場址微生物菌相,甚至目標污染物之降解功能性基因與菌株有一定的掌握,以瞭解場址是否具有生物整治潛勢。高氯數氯乙烯類化合物的降解主要以厭氧還原脫氯為主,著名菌屬Dehalococcoides不同菌種具有不同還原脫氯功能性基因(vcrA、bvcA與tceA),本研究針對污染場址的菌屬Dehalococcoides暨其還原脫氯功能性基因進行分析與定量;另使用次世代定序技術分析場址枯、豐水期菌相是否產生差異,特別是還原脫氯及產氫菌群之變化;並使用螢光染劑Acridine orange (AO
ទីតាំងជាទូទៅនៃការទទួលយកបានភាគច្រើនជា root នៃ មែកធាងនៃជីវិត គឺរវាង monophyletic ដែន បាក់តេរី និង clade បានបង្កើតឡើងដោយ អាកឃី និង ការី នៃអ្វីដែលត្រូវបានសំដៅដល់ថាជា«ដើមឈើប្រពៃណីនៃជីវិត ដោយផ្អែកលើការសិក្សាម៉ូលេគុលជាច្រើន។ [៣៩] [៤០] [៤១] [៤២] [៤៣] [៤៤] មួយជនជាតិភាគតិចតូចណាស់នៃការសិក្សាបានសន្និដ្ឋានខុសគ្នាគឺថាជា root គឺស្ថិតនៅក្នុងបាក់តេរីដែនទាំងនៅក្នុង phylum នេះ Firmicutes [៤៥] ឬថា phylum នេះ Chloroflexi គឺ ...
Obradović, Davor, et al. A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160. PloS one 11.7 (2016): e0159231.. Hemp, James, et al. Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia. Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. Draft Genome of ...
Test kit for rapid identification and quantification of the problem-causing Chloroflexi as well as Eikelboom Type 1851 filaments in wastewater samples.
A single nucleotide primer extension (SNuPE) assay in combination with taxon-specific 16S rRNA gene PCR was developed for the detection and typing of Dehalococcoides populations. Specificity of the assay was evaluated with an isolate and an environmental 16S rRNA gene sequence representing two Dehalococcoides subgroups, i.e. the Pinellas and the Cornell subgroups. Only one sequence type belonging to the Pinellas subgroup was detected in a Bitterfeld/Wolfen region aquifer containing chlorinated ethenes as main contaminants. The three primers hybridization assay thus provided a fast and easy to implement confirmation of the specificity of taxon-specific PCR and allowed a rapid additional taxonomic affiliation into subgroups. This study demonstrates the great potential of SNuPE as a novel approach for rapid parallel detection and typing of different nucleic acid signature sequences from environmental samples ...
JPT Peptide Technologies is a DIN ISO 9001:2015 certified and GCLP compliant integrated provider of innovative peptide based catalog products and custom services.
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
The enrichment culture SL2 dechlorinating tetrachloroethene (PCE) to ethene with strong trichloroethene (TCE) accumulation prior to cis-1,2-dichloroethene (cis-DCE) formation was analyzed for the presence of organohalide respiring bacteria and reductive dehalogenase genes (rdhA). Sulfurospirillum-affiliated bacteria were identified to be involved in PCE dechlorination to cis-DCE whereas Dehalococcoides-affiliated bacteria mainly dechlorinated cis-DCE to ethene. Two rdhA genes highly similar to tetrachloroethene reductive dehalogenase genes (pceA) of S. multivorans and S. halorespirans were present as well as an rdhA gene very similar to the trichloroethene reductive dehalogenase gene (tceA) of Dehalococcoides ethenogenes strain 195. A single strand conformation polymorphism (SSCP) method was developed allowing the simultaneous detection of the three rdhA genes and the estimation of their abundance. SSCP analysis of different SL2 cultures showed that one pceA gene was expressed during PCE
We have cloned the linD genes as a candidate for direct involvement in the degradation of 2,5-DCHQ, the intermediate of γ-HCH degradation by S. paucimobilis UT26. The fact that E. coli cells overproducing LinD converted 2,5-DCHQ to HQ and that the Tn5-induced mutants which show deficiencies in LinD activity have a deletion or rearrangements in the linD gene support the hypothesis that the linD gene is directly involved in 2,5-DCHQ degradation in UT26. Recently, we revealed that rearrangements occurred in the linD gene in UT103, which has no LinD activity (13).. Northern blot analysis revealed that linD mRNA is expressed upon induction with 2,5-DCHQ, whereas linA, linB, and linC are constitutively expressed. This suggested the existence of a regulatory system for linD gene expression.. TLC analysis revealed that LinD rapidly converts 2,5-DCHQ to CHQ and slowly converts CHQ to HQ. This indicates that LinD catalyzes the reductive dehalogenation of 2,5-DCHQ. However, the conversion of CHQ to HQ may ...
Methodology from Fullerton and Moyer (2016). See paper for references cited below.. Sample collection. Subsurface sediments were collected on IODP expedition 331 (Deep Hot Biosphere) from 1 September through 4 October 2010 (Fig. 1). Onboard contamination testing of sites C0015 (126°53=E, 27°47=N; hole B; section 1H-5; 5.6mbsf) and C0017 (126°55=E, 27°47=N; hole C; section 1H-7; 26.6 m bsf) found no indication of interior-core contamination using fluorescent microspheres (both holes C0015B and C0017C) and perfluorocarbon tracer (hole C0017C only). The sample from hole C0017C was also verified by PCR-generated phylotype comparisons based on 97% similarity to phylotypes obtained from drilling mud at a contamination level of 1% or less (26). Subsamples were aseptically collected from the interiors of whole-round cores and stored in cryovials with 27% (vol/vol) glycerol at -80°C.. Single-cell source. Core depths were chosen from sites C0015 and C0017, which were characterized as weakly oxidized ...
All groups of dioxin-like compounds are persistent in the environment.[61] Very few soil microbes nor animals are able to break down effectively the PCDD/Fs with lateral chlorines (positions 2,3,7, and 8). This causes very slow elimination. However scientists at Martin Luther University recently found that a type of bacteria Dehalococcoides CBDB1 can extract the chlorine from dioxin compounds in the absence of oxygen.[62][63] Ultraviolet light is able to slowly break down these compounds. Lipophilicity (tendency to seek for fat-like environments) and very poor water solubility make these compounds move from water environment to living organisms having lipid cell structures. This is called bioaccumulation. Increase in chlorination increases both stability and lipophilicity. The compounds with the very highest chlorine numbers (e.g. octachlorodibenzo-p-dioxin) are, however, so poorly soluble that this hinders their bioaccumulation.[61] Bioaccumulation is followed by biomagnification. Lipid-soluble ...
TY - BOOK. T1 - Enhanced reductive dechlorination in clay till contaminated with chlorinated solvents. AU - Damgaard,Ida. A2 - Broholm,Mette Martina. A2 - Bjerg,Poul Løgstrup. PY - 2012. Y1 - 2012. N2 - Chlorinated solvents are among the most frequently found contaminants in groundwater. In fractured media, chlorinated ethenes and ethanes are transported downwards through preferential pathways with subsequent diffusion into the sediment matrix. Due to slow back diffusion it can serve as a long term secondary source that can leach to the underlying aquifer. As some of the chlorinated solvents and their degradation products are toxic and carcinogenic, remediation technologies applicable in low permeability settings are needed. Enhanced reductive dechlorination (ERD) has been proven efficient in high permeability aquifers and has also been applied at a number of low permeability clay till sites. This thesis presents the results of an investigation of chlorinated ethenes (and ethanes) degradation ...
Sutcliffe, Iain C. (2010). A phylum level perspective on bacterial cell envelope architecture. Trends in Microbiology. 18 (10): 464-470. doi:10.1016/j.tim.2010.06.005. ISSN 0966-842X ...
Can the Acacia Strain pull off one final victory to cement their place in the Death Match Hall of Fame? Or will Ill Nino snatch away a win to begin their own trail to the Hall? The choice is yours. Vote for your favorite track below!. (This Death Match ends on Tuesday, Nov. 13, at 10AM ET. Fans can vote once per hour! So come back and vote often to make sure your favorite song wins!) ...
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
DUGi: Browsing titles in the following repositories: DUGiDocs, DUGiMedia, DUGiFonsEspecials, DUGiImatges, RACO, TDX, MDX, Recercat and MDC. From 0 to 11. And with the following query: dc.subject:Ecophysiology
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
In our research we are developing new isotope and microscopy techniques for the investigation of biogeochemical processes. We want to understand the anaerobic microbiological processes involved in the natural attenuation of hydrocarbons and halogenated organic compounds and the new emerging contaminants in various ecosystems on the microbiological, biochemical and genetic level in order to quantify ecosystems services. For example, we aim to fundamentally understand key degraders such as Dehalococcoides mccartyi involved in the detoxification of organohalides and synthrophic interactions in microbial communities leading to hydrocarbon removal and the biogeochemical cycles of C, N, P, S and various metals in terrestrial systems. We analyse microbially-mediated corrosion to mitigate damage to gas and oil infrastructure. Knowledge of mineral corrosion helps us to develop bioleaching processes with which to extract metals and rare earth elements ...
Do you suffer from symptoms after eating? Such as digestive symptoms, foggy brain, sinus congestion or aches and pains. Do you notice it after gluten containing foods or just cant track whats causing it? Chances are youre body is reacting to components in plants called lectins.. What are lectins? So happy you asked!. Lectins are the plants natural compounds to ward off pests, fungal and bacterial attack. That is, the plants natural immune system. When plants are under attack they raise their lectin numbers to fend off the attacking pests. This is the system by which pesticides work. However, pests are gaining tolerance resulting in needing an increase of pesticide to be applied to the plant and thus a further increase of the plants lectins.. Modern wheat strains vs old style grains. Our modern wheat strains have been modified to produce more lectins to be highly pest resistant. This very clever genetically engineering won Norman Borlaug a Nobel peace prize in 1970 and changed the industry ...
Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular design of quantitative PCR primers/probes where we took advantage of the mosaic aspect of the bacterial genomes to achieve a highly specific quantitative PCR detection system.
It can be hard to tell the difference between a groin strain (muscle or tendon tear) and an inguinal hernia (fat or intestine poking through a hole in the…
From genomic libraries of purple sulphur bacteria, fragments were cloned that encoded for proteins involved in the synthesis of poly(3-hydroxyalkanoic acids), PHA. A 12.5- and a 15.0- plus a 15.6-kbp
Rabbit monoclonal antibody raised against a human TCEA3 peptide using ARM Technology. A synthetic peptide of human TCEA3 is used for rabbit immunization.Customer or Abnova will decide on the preferred peptide sequence. (H00006920-K) - Products - Abnova
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Thompson LR, Sanders JG, McDonald D, and the Earth Microbiome Project Consortium including Seyler LM, and Kerkhof LJ. 2017. A communal catalogue reveals Earths multiscale microbial diversity. Nature. doi: 10.1038/nature24621. Liu, J., Lopez, N., Ahn, Y., Goldberg, T., Bromberg, Y., Kerkhof, L. J. and Häggblom, M. M. 2017. Novel reductive dehalogenases from the marine sponge associated bacterium Desulfoluna spongiiphila. Environmental Microbiology Reports. doi:10.1111/ 1758-2229.12556. Rodenburg LA, Dewani Y, Häggblom MM, Kerkhof LJ, and Fennell DE. 2017. Forensic Analysis of Polychlorinated Dibenzo-p-Dioxin and Furan Fingerprints to Elucidate Dechlorination Pathways. Environ. Sci. Technol. doi: 10.1021/acs.est.7b02705. Kerkhof LJ, Dillon KP, Häggblom MM, McGuinness LR. 2017. Profiling bacterial communities by MinION sequencing of ribosomal operons. Microbiome. doi : 10.1186/s40168-017-0336. Hotaling, L. McDonnell, J., Ferraro, C., Litchenwalner, C.S., and Florio, K. (2017). Educating with ...
Methodology/Principal Findings: Here, we report molecular monitoring of the stone-autochthonous microbiota before and at 5, 12 and 30 months after the bio-consolidation treatment (medium/long-term monitoring), employing the well-known molecular strategy of DGGE analyses. Before the bio-consolidation treatment, the bacterial diversity showed the exclusive dominance of Actinobacteria (100%), which decreased in the community (44.2%) after 5 months, and Gamma-proteobacteria (30.24%) and Chloroflexi (25.56%) appeared. After 12 months, Gamma-proteobacteria vanished from the community and Cyanobacteria (22.1%) appeared and remained dominant after thirty months, when the microbiota consisted of Actinobacteria (42.2%) and Cyanobacteria (57.8%) only. Fungal diversity showed that the Ascomycota phylum was dominant before treatment (100%), while, after five months, Basidiomycota (6.38%) appeared on the stone, and vanished again after twelve months. Thirty months after the treatment, the fungal population ...
Chlorinated compounds such as trichloroethene (TCE) are recalcitrant contaminants commonly detected in soil and groundwater. Contemporary remedies such as electron donor amendment tend to be less or ineffective in treating chlorinated compounds in ma
Temperature cycling is the most common cause of electronic failure. It is typically caused by a coefficient of thermal expansion (CTE) mismatch between the PCB components and the board. The greater the CTE mismatch between the components and the board, the greater the likelihood of solder joint failure.. Download the Thermal Management Solutions: How Hot is Too Hot Whitepaper to learn more.. However, failures can also be caused by localized events. For example, in automotive electronics the PCB is frequently over-constrained within an aluminum housing. The cold side of the PCB will shrink, or the hot side will expand, or both, leading to board buckling.. To analyze for localized events like this, you typically want to run a strain vs. strain comparison, which is an analysis of the board without housing and another analysis of the board inside the housing. This will help determine the increase in the lead strains due to the chassis/enclosure ...
uuid: e4ad4307-37a1-4183-beec-921ec07a361c, type: records, etag: 6cb890cbdb5221e54b3677b2174a3b0f4be4b3a9, data: { dwc:startDayOfYear: 211, dwc:specificEpithet: ilicina, dwc:kingdom: Fungi, dwc:recordedBy: I.M. Brodo; W.B. Schofield, dwc:order: Arthoniales, dwc:habitat: along a shallow stream and shore in Thuja-Alnus forest; at edge of beach; Alnus sinuata, dwc:scientificNameAuthorship: Taylor, dwc:occurrenceID: e4ad4307-37a1-4183-beec-921ec07a361c, id: 69977, dwc:stateProvince: British Columbia, dwc:eventDate: 1967-07-30, dwc:collectionID: ca9232e9-9401-4c90-9845-d00fe37f0ac2, dwc:country: Canada, idigbio:recordId: urn:uuid:e4ad4307-37a1-4183-beec-921ec07a361c, dwc:decimalLatitude: 53.6333333, dwc:basisOfRecord: PreservedSpecimen, dwc:genus: Arthonia, dwc:family: Arthoniaceae, dc:rights: http://creativecommons.org/licenses/by-nc/3.0/, dwc:identifiedBy: T.L. Esslinger: Checklist of North America, 2001, ...
SEQUENCE 494 AA; 53975 MW; 18723DD152CBDB14 CRC64; MVLLAGTGPE GGGARCMTPP PPSPPRGAQV EEDPADYEEF EDFSSLPDTR. SIASDDSFYP FEDEEEHGVE SAESVPEGVP ESVPETATLL RAACANNVGL. LRTLVRRGVS VEEAQETDRN GRTGLIVACY HGFVDTVVAL AECPHVDVNW. QDSEGNTALI TAAQAGHAII TNYLLNYFPG LDLERRNAFG FTALMKAAMQ. GRTDCIRALM LAGADVHARD PRRGMSPQEW ATYTGRVDAV RLMQRLLERP. CPEQFWEKYR PELPPPPEAA RKPAGSKNCL QRLTDCVLSV LTPRSVRGPE DGGVLDHMVR MTTSLYSPAV AIVCQTVCPE SPPSVGKRRL AVQEILAARA. ARGPQAQEED EVGGAGQRGR TGQEDADSRE GSPRAGLPPA LGSRGPAAPA. PRKASLLPLQ RLRRRSVRPG VVVPRVRVSK APAPTFQPER PARKGSTKDS. GHLQIPKWRY KEAKEEKRKA EEAEKKRQAE AQKERRTAPW KKRT ...
Fungal co-life, omics and ecophysiology group studies the ecophysiology, genomics, transcriptomics and metabolism of wood-decaying forest fungi.
Invertebrates - Ecophysiology and Management. Edited by: Sajal Ray, Genaro Diarte-Plata and Ruth Escamilla-Montes. ISBN 978-1-83968-551-4, eISBN 978-1-83968-552-1, PDF ISBN 978-1-83968-553-8, Published 2020-01-22
Hoogewijs, D. , Terwilliger, N. B. , Webster, K. A. , Powell-Coffman, J. A. , Tokishita, S. , Yamagata, H. , Hankeln, T. , Burmester, T. , Rytkönen, K. T. , Nikinmaa, M. , Abele, D. , Heise, K. , Lucassen, M. , Fandrey, J. , Maxwell, P. H. , Pahlman, S. and Gorr, T. A. (2007 ...
As the Regions only Level 1 Trauma and Burn center, the Upstate Golisano Childrens Hospital offers a complete range of health care services for children from birth to 19 years of age. Our dedicated pediatric providers and staff care for patients in an inpatient setting that consists of General Pediatrics, Pediatric Surgery Unit, Hematology/ Oncology, and the only Pediatric Intensive Care Unit in our large referral area and the only dedicated pediatric Emergency Department between Rochester and Vermont. In addition, the Childrens Hospital offers full spectrum of ambulatory services including primary care and several nationally recognized specialty medical care programs.. Browse the Pediatric Services and Clinics A-Z list to find a comprehensive list of clinics providing services. Use the Find a Doctor link to get more information about a doctor or to search for doctors with a particular specialty or who treat specific diseases. ...
Chloroflexi[edit]. Main article: Chloroflexi (phylum). Chloroflexi, a diverse phylum including thermophiles and halorespirers, ... Green non-sulfur bacteria and relatives (later renamed Chloroflexi[24]) *Chloroflexus group (Chloroflexus, Herpetosiphon) ... However, the candidate phylum TM7 may also be Gram positive.[16] Chloroflexi however possess a single bilayer, but stain ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ...
Chloroflexi phy. nov.". Bergey's Manual® of Systematic Bacteriology. Bergey's Manual of Systematic Bacteriology. New York, NY: ...
Nov., within the phylum Chloroflexi". International Journal of Systematic and Evolutionary Microbiology. 63 (Pt 2): 625-35. doi ...
The Chloroflexi have a single layer, yet (with some exceptions) stain negative. Two related phyla to the Chloroflexi, the TM7 ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ... Chloroflexi, etc.). The presence of this CSI in all sequenced species of conventional LPS (lipopolysaccharide)-containing gram- ...
nov., within the phylum Chloroflexi". International Journal of Systematic and Evolutionary Microbiology. 63 (Pt 2): 625-635. ...
Chloroflexi are the deepest branching (oldest) anoxygenic phototrophs on the tree of life. Many of the species in Chloroflexi ... There are six classes within the phylum Chloroflexi: Chloroflexi, Anaerolinea, Caldilinea, Dehalococcoidia (previously known ... Chloroflexi consists of the green non-sulfur bacteria which are anoxygenic phototrophs (do not produce oxygen during ... Yan, J.; Rash, B.A.; Rainey, F.A.; Moe, W.M. (2009). "Isolation of novel bacteria within the Chloroflexi capable of reductive ...
Chloroflexi), purple bacteria, acidobacteria, and heliobacteria. Some archaea (e.g. Halobacterium) capture light energy for ...
The G/C content for Chloroflexus islandicus was found to be 59.6mol%. Chloroflexi Endosymbiotic theory Gaisin, V. A.; ...
Chloroflexi), the species stains Gram negative, yet has a single lipid layer (monoderm), but with thin peptidoglycan, which is ... Chloroflexi endosymbiotic theory Parte, A.C. "Chloroflexus". LPSN. Sutcliffe, I. C. (2010). "A phylum level perspective on ...
February 2020). "Extremophilic nitrite-oxidizing Chloroflexi from Yellowstone hot springs". The ISME Journal. 14 (2): 364-379. ... Proteobacteria and Chloroflexi. They are present in soil, geothermal springs, freshwater and marine ecosystems. Ammonia ...
This trait is found in some proteobacteria, chloroflexi (green nonsulfur bacteria), low G+C gram positive Clostridia. and ... Reductive dechlorination Chloroflexi (phylum) Dehalococcoides Dehalobacter Holliger, C.; Wohlfarth, G.; Diekert, G. (1998). " ...
Chlorobiaceae and Chloroflexi) and Cyanobacteria. Purple and green bacteria oxidize sulfide, sulfur, sulfite, iron or hydrogen ...
Both families possess members from Firmicutes, Actinobacteria and Chloroflexi. Proteins of this superfamily all appear to have ...
... is a genus in the phylum Chloroflexi (Bacteria). The name Dehalogenimonas derives from: Latin prep. de, away, ...
One of the features of the members of the phylum Chloroflexi is the unusual cell wall structure, which is monoderm but with ... Dehalococcoides is a class of Chloroflexi, a phylum of Bacteria. It is also known as the DHC group. The name Dehalococcoidetes ... in the phylum Chloroflexi (emended description)". International Journal of Systematic and Evolutionary Microbiology. 54 (6): ...
The main types of microbe found there are Chloroflexi and Acidobacteria. Mount Erebus is large enough to have several named ...
... is one of two orders of bacteria in the class Chloroflexi. List of bacterial orders Garrity, George M. (2001). ...
Prokaryotic photoautotrophs include Cyanobacteria, Proteobacteria, Chloroflexi, Acidobacteria, Chlorobi, Firmicutes, ...
2010 This section lists the orders of Bacteria within the phylum Chloroflexi. Anaerolineales Yamada et al. 2006 ...
They are also found in isolated species of Bacteroidetes, Chloroflexi and deltaproteobacteria. Several lines of evidence led to ...
Thomas Cavalier-Smith hypothesizes that the phenotypically diverse phylum Chloroflexi contained the LUCA. In 2005, Peter Ward ...
Phototrophic bacteria are found in the phyla Cyanobacteria, Chlorobi, Proteobacteria, Chloroflexi, and Firmicutes. Along with ...
nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment". International Journal ...
nov., a filamentous, anaerobic bacterium belonging to the class Anaerolineae in the phylum Chloroflexi". International Journal ...
In anoxic sediments, hydrogen is an important "edible". Members of the Chloroflexi draw energy from it to produce acetate by ... It includes strict anaerobes including members of the Chloroflexi phylum, "Ca. Atribacteria", sulfate-reducing bacteria, and ... Chloroflexi and Planctomycetes. Members of Archaea were first identified using metagenomic analysis; some of them have since ... Chloroflexi, Marine Group II archaea and lithoautotrophs in the Thaumarchaeota phylum. Fungi are diverse, including members of ...
"Arsenite oxidase gene diversity among Chloroflexi and Proteobacteria from El Tatio Geyser Field, Chile". FEMS Microbiology ...
nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading ...
Filamentous anoxygenic phototrophs of the phylum Chloroflexi lack the FMO complex, but instead use a protein complex called ... Other Csm proteins with different letter suffixes can be found in Chloroflexi and Ca. Chloracidobacterium. Within the ...
... green nonsulfur bacteria. Version 10 March 2006 (temporary). http://tolweb.org/Chloroflexi/2289/2006.03.10 in The ... in the phylum Chloroflexi (emended description). International Journal of Systematic and Evolutionary Microbiology 54(6):2049- ...
Chloroflexi is a temporary taxon name for: Chloroflexi (phylum) Chloroflexi (class) This disambiguation page lists articles ...
"Chloroflexi". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved 2018-07-20. Sayers; et al. "Chloroflexi ... It has been suggested that the phylum Chloroflexi sensu stricto should comprise only the classes Chloroflexi and Thermomicrobia ... "Chloroflexi" in Volume One of Bergeys Manual of Systematic Bacteriology. Chloroflexi being a deep branching phylum (see ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes, including ...
It has been suggested that the phylum Chloroflexi sensu stricto should comprise only the classes Chloroflexi and Thermomicrobia ... The name Chloroflexi is a Neolatin nominative case masculine plural of Chloroflexus, which is the name of the first genus ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes, including ... Chloroflexi being a deep branching phylum (see Bacterial phyla), it was considered in Volume One of Bergeys Manual of ...
The phototrophic Chloroflexi carry out anoxygenic photosynthesis; they have a single type of photosystem and carry out cyclic ... General characteristics of the Chloroflexi. Diversity. Just over a dozen genera, with only one or sometimes two species in each ... Chloroflexus contains chlorosomes and bacteriochlorophyl c, whereas other Chloroflexi do not. These are traits otherwise found ... These chlorosomes resemble those of the Chlorobi (see below), but phototrophy in Chloroflexi otherwise resembles that of ...
Incubation with 13C-acetate and nanoSIMS (secondary ion mass-spectrometry) indicated higher uptake in both Chloroflexi and SRBs ... of Chloroflexi in GN-S mats (Ley et al., 2006) as well as a similar level of Chloroflexi pyrotags seen in GN-I mats. Moreover, ... Chloroflexi (Figure 1C), was divided between the phototrophic (class Chloroflexi) and the dark filamentous clades of the ... Chloroflexi (CFX1223 and GNSB-941) (A-D) and Desulfosarcina/Desulfobacteraceae (DSS658) hybridized filaments (E-H) shown in ...
Ang Chloroflexi ay isang uri ng phylum sa bakterya kahariang Protista. Hindi malaman kung saan ito maikakategorya, sa Gram- ... Kinuha mula sa "https://tl.wikipedia.org/w/index.php?title=Chloroflexi_(phylum)&oldid=1477440" ...
... ISME J ... we report on the discovery of a chemolithoautotrophic nitrite oxidizer that belongs to the widespread phylum Chloroflexi not ...
Cultivation of uncultured Chloroflexi subphyla: significance and ecophysiology of formerly uncultured Chloroflexi subphylum I ... It is likely that similar subsurface Chloroflexi have the potential for growth, since Chloroflexi sequences were recovered from ... Our nine Chloroflexi SAGs (seven of which are from the order Anaerolineales) indicate that, in addition to genes for the Wood- ... Nine Chloroflexi SSU rRNA gene sequences were recovered, four from hole C0015B and five from hole C0017C. Of the nine ...
Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean. R. M. ... 1). Within the Chloroflexi phylum, the closest relatives to the SAR202 cluster could not be identified with the 16S rRNA gene ... Prevalence of the Chloroflexi-Related SAR202 Bacterioplankton Cluster throughout the Mesopelagic Zone and Deep Ocean ... Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi. While members of this diverse group are ...
nov., within the phylum Chloroflexi. Int J Syst Evol Microbiol 63:625-635. doi:10.1099/ijs.0.034926-0. ... Cell envelope architecture in the Chloroflexi: a shifting frontline in a phylogenetic turf war. Environ Microbiol 13:279-282. ... Genome sequencing of a single cell of the widely distributed marine subsurface Dehalococcoidia, phylum Chloroflexi. ISME J 8: ... Detection of dsr genes in DEH bacteria indicates a potential for sulfite reduction in members of the phylum Chloroflexi and ...
The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep ... Chloroflexi CL500-11 Populations That Predominate Deep-Lake Hypolimnion Bacterioplankton Rely on Nitrogen-Rich Dissolved ... Chloroflexi CL500-11 Populations That Predominate Deep-Lake Hypolimnion Bacterioplankton Rely on Nitrogen-Rich Dissolved ... Denef, V. J., Mueller, R. S., Chiang, E., Liebig, J. R., & Vanderploeg, H. A. (2016). Chloroflexi CL500-11 populations that ...
Chloroflexi abundance in HMA sponges.Recently, members of the phylum Chloroflexi were shown to be present in much higher ... Red clusters represent sponge-specific Chloroflexi cluster, whereas light gray clusters are built by nonsymbiotic Chloroflexi ... Central metabolism of sponge-associated Chloroflexi.Metabolic reconstruction suggests that Chloroflexi are aerobic and ... Cultivation of uncultured Chloroflexi subphyla: significance and ecophysiology of formerly uncultured Chloroflexi "subphylum I ...
f Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide ... Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide ...
strain MS-G (Chloroflexi). Authors: Schuster, Stephan Christoph. Bryant, Donald A.. Gay, Scott E.. Thiel, Vera. Hamilton, ... strain MS-G (Chloroflexi), isolated from Mushroom Spring (Yellowstone National Park, WY, USA) was sequenced and comprises ... strain MS-G (Chloroflexi). Genome Announcements, 2(5), e00872-14-. doi:10.1128/genomeA.00872-14. ...
Chloroflexi[edit]. Main article: Chloroflexi (phylum). Chloroflexi, a diverse phylum including thermophiles and halorespirers, ... Green non-sulfur bacteria and relatives (later renamed Chloroflexi[24]) *Chloroflexus group (Chloroflexus, Herpetosiphon) ... However, the candidate phylum TM7 may also be Gram positive.[16] Chloroflexi however possess a single bilayer, but stain ... Sutcliffe, I. C. (2011). "Cell envelope architecture in the Chloroflexi: A shifting frontline in a phylogenetic turf war". ...
Chloroflexi.. The most dominant phylotype (Ced_B01) in the GPS1 belongs to the clade of candidate order MSBL5 in the phylum ... S2 and Table S1) (7). The lowest species richness was seen in GPS1, where the community was dominated by phyla Chloroflexi and ... 1 and Table S1). NS1 and BS5 also contained phyla of Chloroflexi and Firmicutes, but were dominated by a class ... In the spring fed solely by deep groundwater, phylum Chloroflexi, class Clostridia, and candidate division OD1 were the major ...
Draft Genome Sequence of a Divergent Anaerobic Member of the Chloroflexi Class Ardenticatenia from a Sulfidic Hot Spring L. M. ...
Test kit for rapid identification and quantification of the problem-causing Chloroflexi as well as Eikelboom Type 1851 ... VIT® Chloroflexi enables the fast and specific identification of the group of Chloroflexi filaments and of Eikelboom Type 1851 ... VIT® Chloroflexi. Test kit for rapid identification and quantification of filamentous bacteria. Product Number: 01110022 ... The Eikelboom Type 1851 for example is part of the Chloroflexi group and known as being problematic for sludge settling. ...
Chloroflexi[edit]. Bacteria in this phyla have a variety of extremophile traits. Some are moderate thermophiles, aerobic, ... These bacteria are extremophiles that consist of the phyla: Aquificae, Thermotogae, Thermus-Deinococcus, and Chloroflexi. These ...
It has been suggested that the phylum Chloroflexi "sensu stricto" should comprise only the classes Chloroflexi and ... The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes including members ... "Chloroflexi". List of Prokaryotic names with Standing in Nomenclature (LPSN) [1]. Retrieved 2014-03-20. ... "Chloroflexi". National Center for Biotechnology Information (NCBI) taxonomy database [2]. Retrieved 2014-03-20. ...
Chloroflexi. (redirected from Chlorobacteria) Chloroflexi. A prokaryote genus name with no current standing in prokaryotic ...
Chloroflexi have been implicated in BNR systems by phylogenetic identification of filamentous bacteria isolated by ... Specific 16S rRNA-targeted oligonucleotide probes were designed for the phylum Chloroflexi (green non-sulfur bacteria) and ... Filamentous bacteria responding to a subdivision 1 Chloroflexi probe were rare in the samples, whereas subdivision 3 ... It is suggested that filamentous bacteria belonging to the Chloroflexi phylum account for a large fraction of phylogenetically ...
... sequences revealed that strain PRI-4131T represented a distinct lineage within the class Caldilineae of the phylum Chloroflexi ... nov., an aerobic member of the class Caldilineae , phylum Chloroflexi , isolated from an intertidal hot spring * Varsha Kale1,2 ... Blazejak A., Schippers A. ( 2010 ). High abundance of JS-1- and Chloroflexi-related bacteria in deeply buried marine sediments ... in the bacterial phylum Chloroflexi . . Int J Syst Evol Microbiol 56, 1331-1340. [CrossRef] [PubMed] ...
Cultivation of uncultured chloroflexi subphyla: significance and ecophysiology of formerly uncultured chloroflexi subphylum i ... in the bacterial phylum Chloroflexi.. Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi A, Harada H, Kamagata Y., Int. J. Syst ... Among them, the Chloroflexi comprises taxonomically and physiologically diverse lineages adapted to a wide range of aquatic and ... Chloroflexi bacterium oral taxon 439 isolate Chl1-2 contig_116, whole genome shotgun sequence. (EMBL: APVK01000113). Sequence ...
There are six classes within the phylum Chloroflexi: Chloroflexi, Anaerolinea, Caldilinea, Dehalococcoidia (previously known ... nov., within the phylum Chloroflexi" (PDF). Int. J. Syst. Evol. Microbiol. 63 (Pt 2): 625-635. doi:10.1099/ijs.0.034926-0. PMID ... Many of the species in Chloroflexi are thermophilic however Dehalogenimonas lykanthroporepellens is a mesophile.[7] The ... lykanthroporepellens also differ from other species in the phylum Chloroflexi in that they are not filamentous.[7] ...
Chloroflexi (including TK10) is abundant in HG rhizosphere soil. It seems to play a useful role in the soil in providing the ... Speirs, L. B. M., Rice, D. T. F., Petrovski, S. & Seviour, R. J. The phylogeny, biodiversity, and ecology of the chloroflexi in ... On the other hand, the Deltaproteobacteria; Chloroflexi; Myxococcales; Gemmatimonadetes; Gemmatimonadales; Gemmatimonadaceae; ...
Based on BLAST analysis, about 93% (62 sequences) of the 67 Chloroflexi sequences were homologous to Chloroflexi phylotypes ... Chloroflexi has been detected in the human oral cavity of patients suffering from peri-implantitis and in the vagina of ... Interestingly, Chloroflexi is also present in various environments such as freshwater lakes, soil, deep-sea sponges, hot ... The goal of this study was to better characterize Chloroflexi from activated wastewater, where it is known to be abundant, in ...
Chloroflexi Dehalococcoides. 1.47. 0.49. 1641. 1591. 51. 1. 1426. 628. 17. Dehalococcoides sp. BAV1. Chloroflexi ... Chloroflexi Dehalococcoides. 1.39. 0.47. 1516. 1458. 58. 1. 1378. 541. 32. Dehalococcoides sp. VS. Chloroflexi ... Yan, J., Rash, B.A., Rainey, F.A., and Moe, W.M. (2009) Isolation of novel bacteria within the Chloroflexi capable of reductive ... and related isolates within the Chloroflexi represent a special case in the anaerobic detoxification of halogenated organic ...
Background Mosquitoes host diverse microbial communities that influence many aspects of their biology including reproduction, digestion, and ability to transmit pathogens. Unraveling the composition,...
  • 2004. Reclassification of Sphaerobacter thermophilus from the subclass Sphaerobacteridae in the phylum Actinobacteria to the class Thermomicrobia (emended description) in the phylum Chloroflexi (emended description). (tolweb.org)
  • Chloroflexi is a temporary taxon name for: Chloroflexi (phylum) Chloroflexi (class) This disambiguation page lists articles about taxa associated with the same name. (wikipedia.org)
  • In contrast, the members of the phylum Chloroflexi are monoderms, but stain mostly gram-negative. (wikipedia.org)
  • Chloroflexi being a deep branching phylum (see Bacterial phyla), it was considered in Volume One of Bergey's Manual of Systematic Bacteriology to include a single class with the same name, the class Chloroflexi. (wikipedia.org)
  • Since 2001, however, new classes have been created thanks to newly discovered species, and the phylum Chloroflexi is now divided as follows: Chloroflexi Gupta et al. (wikipedia.org)
  • Recent phylogenetic analysis of the Chloroflexi has found very weak support for the grouping together of the different classes currently part of the phylum. (wikipedia.org)
  • It has been suggested that the phylum Chloroflexi sensu stricto should comprise only the classes Chloroflexi and Thermomicrobia, and the other four classes ("Dehalococcoidetes," Anaerolineae, Caldilineae and Ktedonobacteria) may represent one or more independent phyla branching in the neighborhood of the Chloroflexi. (wikipedia.org)
  • Ang Chloroflexi ay isang uri ng phylum sa bakterya kahariang Protista . (wikipedia.org)
  • Here, we report on the discovery of a chemolithoautotrophic nitrite oxidizer that belongs to the widespread phylum Chloroflexi not previously known to contain any nitrifying organism. (nih.gov)
  • Chloroflexi small-subunit (SSU) rRNA gene sequences are frequently recovered from subseafloor environments, but the metabolic potential of the phylum is poorly understood. (asm.org)
  • The phylum Chloroflexi , colloquially known as the green nonsulfur bacteria, has been recognized as both ubiquitous and diverse using small-subunit (SSU) rRNA in molecular studies ( 1 ). (asm.org)
  • Together, these clones form a major, monophyletic subgroup of the phylum Chloroflexi . (asm.org)
  • Interestingly, SAR202 organisms and relatives are members of the Chloroflexi phylum, one of the 11 original phyla described by comparative 16S rRNA sequence analysis ( 45 ). (asm.org)
  • The marine subsurface sediment biosphere is widely inhabited by bacteria affiliated with the class Dehalococcoidia (DEH), phylum Chloroflexi , and yet little is known regarding their metabolisms. (asm.org)
  • Members of the widespread bacterial phylum Chloroflexi can dominate high-microbial-abundance (HMA) sponge microbiomes. (asm.org)
  • IMPORTANCE Chloroflexi represent a widespread, yet enigmatic bacterial phylum with few cultivated members. (asm.org)
  • The Chloroflexi or Chlorobacteria are a phylum of bacteria containing isolates with a diversity of phenotypes including members that are aerobic thermophiles, which use oxygen and grow well in high temperatures, anoxygenic phototrophs, which use light for photosynthesis, and anaerobic halorespirers, which uses halogenated organics (such as the toxic chlorinated ethenes and polychlorinated biphenyls ) as energy sources. (omicsgroup.org)
  • Whereas most Bacteria, in terms of diversity, are diderms and stain Gram negative with the exception of the Firmicutes (low CG Gram positives), Actinobacteria (high CG gram positives) and the Deinococcus-Thermus group (Gram positive, but diderms with thick peptidoglycan), the members of the phylum Chloroflexi are monoderms , but stain mostly Gram negative. (omicsgroup.org)
  • Specific 16S rRNA-targeted oligonucleotide probes were designed for the phylum Chloroflexi (green non-sulfur bacteria) and optimized for use in fluorescence in situ hybridization. (microbiologyresearch.org)
  • It is suggested that filamentous bacteria belonging to the Chloroflexi phylum account for a large fraction of phylogenetically uncharacterized filaments in BNR systems and are likely to be abundant in such systems on a global scale. (microbiologyresearch.org)
  • Dehalogenimonas lykanthroporepellens is an anaerobic , Gram-negative bacteria in the phylum Chloroflexi isolated from a Superfund site in Baton Rouge , Louisiana . (wikipedia.org)
  • [1] D . lykanthroporepellens also differ from other species in the phylum Chloroflexi in that they are not filamentous . (wikipedia.org)
  • The phylum Chloroflexi is relatively unexplored, with the majority of its representatives being uncultivable. (sjsu.edu)
  • Overall, results suggest that activated wastewater serves as a potential habitat where one can find a human-associated Chloroflexi bacterium analog in order to better characterize this phylum and its contribution to human health. (sjsu.edu)
  • Uncultured bacteria affiliated with the CL500-11 cluster (phylum Chloroflexi) were first reported from the oxygenated hypolimnion of Crater Lake (USA) as a predominant bacterioplankton, although this dominance has not been reported in other environments. (elsevier.com)
  • Recent 16S rRNA gene profiling indicates that members of the bacterial phylum Chloroflexi are common in sediment. (beds.ac.uk)
  • The coexistence of uncultured heterotrophic bacteria belonging to the phylum Chloroflexi has often been observed in anaerobic ammonium oxidation (anammox) reactors fed with synthetic nutrient medium without organic carbon compounds. (hiroshima-u.ac.jp)
  • The sequence similarities to Anaerolinea thermophila and Caldilinea aerophila within the phylum Chloroflexi were only 81.0-88.7% and 80.3-83.8%, respectively. (hiroshima-u.ac.jp)
  • CL500-11 (phylum Chloroflexi) is one of the most ubiquitous and abundant bacterioplankton lineages in deep freshwater lakes inhabiting the oxygenated hypolimnion. (mendeley.com)
  • One thermophilic (strain IMO-1 T ) and two mesophilic (strains KIBI-1 T and YMTK-2 T ) non-spore-forming, non-motile, Gram-negative, multicellular filamentous micro-organisms, which were previously isolated as members of the tentatively named class ' Anaerolineae ' of the phylum Chloroflexi , were characterized. (microbiologyresearch.org)
  • nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment. (marum.de)
  • In 1987, Carl Woese, regarded as the forerunner of the molecular phylogeny revolution, divided Eubacteria into 11 divisions based on 16S ribosomal RNA (SSU) sequences and grouped the genera Chloroflexus, Herpetosiphon and Thermomicrobium into the "green non-sulfur bacteria and relatives", which was temporarily renamed as "Chloroflexi" in Volume One of Bergey's Manual of Systematic Bacteriology. (wikipedia.org)
  • Incubation with 13 C-acetate and NanoSIMS (secondary ion mass-spectrometry) indicated higher uptake in both Chloroflexi and SRBs relative to other filamentous bacteria. (frontiersin.org)
  • The Chloroflexi line of descent is thought by many to have diverged early in the evolution of the domain Bacteria ( 29 ). (asm.org)
  • Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removalccThe EMBL accession numbers for the sequences reported in this paper are X84472 (strain SBR1029 16S rDNA), X84474 (strain SBR1031 16S rDNA), X84498 (strain SBR1064 16S rDNA), X84565 (strain SBR2022 16S rDNA), X84576 (strain SBR2037 16S rDNA) and X84607 (strain SBR2076 16S rDNA). (microbiologyresearch.org)
  • Chloroflexi have been implicated in BNR systems by phylogenetic identification of filamentous bacteria isolated by micromanipulation from sludge and culture-independent molecular phylogenetic surveys. (microbiologyresearch.org)
  • Filamentous bacteria responding to a subdivision 1 Chloroflexi probe were rare in the samples, whereas subdivision 3 Chloroflexi filaments were very common in some sludges. (microbiologyresearch.org)
  • High abundance of JS-1- and Chloroflexi -related bacteria in deeply buried marine sediments revealed by quantitative, real-time PCR. (microbiologyresearch.org)
  • A distinct group of uncultured chloroflexi related to free-living anaerobic Anaerolineae inhabits the mammalian gastrointestinal tract and includes low-abundance human oral bacteria that appear to proliferate in periodontitis. (uni-bielefeld.de)
  • [2] Chloroflexi consists of the green non-sulfur bacteria which are anoxygenic phototrophs (do not produce oxygen during photosynthesis ) that use either H 2 or H 2 S as an electron donor . (wikipedia.org)
  • In these communities, it is the cyanobacteria that are the primary producers, and the Chloroflexi grow photoheterotrophically from organic carbon produced by the cyanobacteria. (ncsu.edu)
  • These manipulations and diel incubations confirm that Cyanobacteria were the main fermenters in Guerrero Negro mats and that the net flux of nighttime fermentation byproducts (not only hydrogen) was largely regulated by the interplay between Cyanobacteria , SRBs, and Chloroflexi . (frontiersin.org)
  • Proteobacteria, Firmicutes, Chloroflexi, and Bacteroidetes were found to be the dominant bacterial phyla in both digester configuration types (with and without recirculation). (thefreedictionary.com)
  • Chloroflexi bacterium oral taxon 439 isolate Chl1-2 contig_116, whole genome shotgun sequence. (uni-bielefeld.de)
  • OS Chloroflexi bacterium TMED70. (genome.jp)
  • These chlorosomes resemble those of the Chlorobi (see below), but phototrophy in Chloroflexi otherwise resembles that of typical purple bacterial photosynthesis. (ncsu.edu)
  • In comparisons among Chloroflexi -containing sites that were rich in methane hydrates and organic carbon off the Peru and Cascade Margins, Chloroflexi SSU rRNA gene sequences were more numerous at the organic-rich sites ( 8 ). (asm.org)
  • Isolation of an environmental Chloroflexi with high 16S rRNA gene homology to a human-associated counterpart could be used as a model to understand its role in human health. (sjsu.edu)
  • Analysis using the 16S rRNA gene clone library generated 230 clones, out of which 67 clones were Chloroflexi. (sjsu.edu)
  • We sampled genomic sequence from 86 Chloroflexi representing 15 distinct lineages, including members of eight classes previously characterized only by 16S rRNA sequences. (beds.ac.uk)
  • Based on their SSU rRNA gene identities, nine Chloroflexi SAGs (of the total 29 unique MDA reactions identified after cell sorting) were chosen for whole-genome sequencing. (bco-dmo.org)
  • Chloroflexus contains chlorosomes and bacteriochlorophyl c , whereas other Chloroflexi do not. (ncsu.edu)
  • Unlike in the Dehalococcoidia, genes for organohalide respiration are rare within the Chloroflexi genomes sampled here. (beds.ac.uk)
  • Using a single-cell genomics approach, we obtained the first draft genomic reconstruction for these organisms and compared their inferred metabolic potential with free-living chloroflexi. (uni-bielefeld.de)
  • Bacterial phyla ) its classification was analysed in Volume One of Bergey's Manual of Systematic Bacteriology and included a single class with the same name, the class Chloroflexi . (omicsgroup.org)
  • Genomic data suggest that oral chloroflexi are anaerobic heterotrophs, encoding abundant carbohydrate transport and metabolism functionalities, similar to those seen in environmental Anaerolineae isolates. (uni-bielefeld.de)
  • It is likely that similar subsurface Chloroflexi have the potential for growth, since Chloroflexi sequences were recovered from an in situ colonization experiment within a borehole, indicating recent growth on previously uninhabited surfaces ( 13 ). (asm.org)
  • In the Sponge Microbiome Project, Chloroflexi sequences amounted to 20 to 30% of the total microbiome of certain HMA sponge genera with the classes/clades SAR202, Caldilineae , and Anaerolineae being the most prominent. (asm.org)
  • Based on BLAST analysis, about 93% (62 sequences) of the 67 Chloroflexi sequences were homologous to Chloroflexi phylotypes from other environmental sites. (sjsu.edu)
  • The remaining 7% (5 sequences) of Chloroflexi sequences were homologous to human-associated phylotypes derived from samples collected on human skin or human oral cavity. (sjsu.edu)
  • Phylogenetic analysis revealed that 36% of the clones analyzed in this study were affiliated with Chloroflexi. (hiroshima-u.ac.jp)
  • Despite this, the Chloroflexi remain a relatively understudied bacterial lineage. (beds.ac.uk)
  • [1] The carbon source has not been determined for this species but other organisms within Chloroflexi use CO 2 as a carbon source. (wikipedia.org)
  • Diversity and genomic insights into the uncultured Chloroflexi from the human microbiota", Environmental Microbiology , vol. 16, 2014, pp. 2635-2643. (uni-bielefeld.de)
  • The goal of this study was to better characterize Chloroflexi from activated wastewater, where it is known to be abundant, in order to elucidate the relatedness of an environmental analog to a potential human pathogen. (sjsu.edu)
  • Chloroflexi in the aquifer sediments are abundant and highly diverse. (beds.ac.uk)
  • In contrast to previously examined Chloroflexi SAGs, our genomes (several are from the order Anaerolineales ) were recovered from a hydrothermally driven system and therefore provide a unique window into the metabolic potential of this type of habitat. (asm.org)
  • The distinctive gene content of this cell suggests metabolic characteristics that differ from those of known DEH and Chloroflexi . (asm.org)
  • The results of this study suggest clade-specific metabolic specialization and that Chloroflexi symbionts have the genomic potential for dissolved organic matter (DOM) degradation from seawater. (asm.org)
  • Genomic sampling is available for only six of the approximate 30 Chloroflexi classes, so little is known about the phylogenetic distribution of reductive dehalogenation or about the broader metabolic characteristics of Chloroflexi in sediment. (beds.ac.uk)
  • We used metagenomics to directly evaluate the metabolic potential and diversity of Chloroflexi in aquifer sediments. (beds.ac.uk)
  • Previous studies of subsurface Chloroflexi single amplified genomes (SAGs) suggested heterotrophic or lithotrophic metabolisms and provided no evidence for growth by reductive dehalogenation. (asm.org)
  • Our nine Chloroflexi SAGs (seven of which are from the order Anaerolineales ) indicate that, in addition to genes for the Wood-Ljungdahl pathway, exogenous carbon sources can be actively transported into cells. (asm.org)
  • At least one subunit for pyruvate ferredoxin oxidoreductase was found in four of the Chloroflexi SAGs. (asm.org)
  • Near-complete genomes were reconstructed for three Chloroflexi. (beds.ac.uk)
  • [1] Chloroflexi are the deepest branching (oldest) anoxygenic phototrophs on the tree of life . (wikipedia.org)
  • Most of the Chloroflexi filaments are uncultivable and unknown, so that knowledge about them is limited. (vermicon.com)
  • The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. (oregonstate.edu)
  • Interestingly, Chloroflexi is also present in various environments such as freshwater lakes, soil, deep-sea sponges, hot springs and activated wastewater. (sjsu.edu)
  • VIT® Chloroflexi enables the fast and specific identification of the group of Chloroflexi filaments and of Eikelboom Type 1851 in sludge samples. (vermicon.com)
  • However, the classes Chloroflexi and Thermomicrobia were found to group together consistently by both the usual phylogenetic means and the identification of shared conserved signature indels in the 50S ribosomal protein L19 and the enzyme UDP-glucose 4-epimerase. (wikipedia.org)
  • [7] The Oscillochloris (Class Chloroflexi ) are also mesophilic . (wikipedia.org)
  • To determine if coexisting Chloroflexi in anammox reactors scavenge organic matter derived from anammox bacterial cells, the present study was conducted to investigate the substrate uptake pattern of the uncultured Chloroflexi present in an anammox reactor and to clarify if they take up microbial products derived from anammox bacterial cells. (hiroshima-u.ac.jp)
  • We expand the potential roles of Chloroflexi in sediment carbon cycling beyond organohalide respiration to include respiration of sugars, fermentation, CO 2 fixation, and acetogenesis with ATP formation by substrate-level phosphorylation. (beds.ac.uk)
  • A metagenomic study of the Peru Margin showed Chloroflexi to be present at a broad range of depths and to compose 12 to 16% of the total genes identified ( 9 ). (asm.org)
  • The presence of genes encoding dissimilatory sulfite reductase (Dsr) suggests that DEH could respire oxidized sulfur compounds, although Chloroflexi have never been implicated in this mode of sulfur cycling. (asm.org)
  • The presence of genes for a unique phosphotransferase system and N-acetylglucosamine metabolism suggests an important ecological niche for oral chloroflexi in scavenging material from lysed bacterial cells and the human tissue. (uni-bielefeld.de)
  • Among them, the Chloroflexi comprises taxonomically and physiologically diverse lineages adapted to a wide range of aquatic and terrestrial habitats. (uni-bielefeld.de)