Chloride Peroxidase
Horseradish Peroxidase
Glutathione Peroxidase
Peroxidase
Cytochrome-c Peroxidase
Chloride Channels
Ascorbate Peroxidases
Eosinophil Peroxidase
Iodide Peroxidase
Vinyl Chloride
Polyvinyl Chloride
Hydrogen Peroxide
Guaiacol
Catalase
Mercuric Chloride
Selenium
Heterologous expression of the vanadium-containing chloroperoxidase from Curvularia inaequalis in Saccharomyces cerevisiae and site-directed mutagenesis of the active site residues His(496), Lys(353), Arg(360), and Arg(490). (1/107)
The vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis is heterologously expressed to high levels in the yeast Saccharomyces cerevisiae. Characterization of the recombinant enzyme reveals that this behaves very similar to the native chloroperoxidase. Site-directed mutagenesis is performed on four highly conserved active site residues to examine their role in catalysis. When the vanadate-binding residue His(496) is changed into an alanine, the mutant enzyme loses the ability to bind vanadate covalently resulting in an inactive enzyme. The negative charges on the vanadate oxygens are compensated by hydrogen bonds with the residues Arg(360), Arg(490), and Lys(353). When these residues are changed into alanines the mutant enzymes lose the ability to effectively oxidize chloride but can still function as bromoperoxidases. A general mechanism for haloperoxidase catalysis is proposed that also correlates the kinetic properties of the mutants with the charge and the hydrogen-bonding network in the vanadate-binding site. (+info)Striking activation of oxidative enzymes suspended in nonaqueous media. (2/107)
The catalytic activity of four lyophilized oxidative enzymes-horseradish peroxidase, soybean peroxidase, Caldariomyces fumago chloroperoxidase, and mushroom polyphenol oxidase-is much lower when directly suspended in organic solvents containing little water than when they are introduced into the same largely nonaqueous media by first dissolving them in water and then diluting with anhydrous solvents. The lower the water content of the medium, the greater this discrepancy becomes. The mechanism of this phenomenon was found to arise from reversible denaturation of the oxidases on lyophilization: because of its conformational rigidity, the denatured enzyme exhibits very limited activity when directly suspended in largely nonaqueous media but renatures and thus yields much higher activity if first redissolved in water. Two independent means were discovered for dramatically minimizing the lyophilization-induced inactivation, both involving the addition of certain types of excipients to the aqueous enzyme solution before lyophilization. The first group of excipients consists of phenolic and aniline substrates as well as other hydrophobic compounds; these presumably bind to the hydrophobic pocket of the enzyme active site, thereby preventing its collapse during dehydration. The second group consists of general lyoprotectants such as polyols and polyethylen glycol that apparently preserve the overall enzyme structure during dehydration. The activation effects of such excipients can reach into the tens and hundreds of fold. Moreover, the activations afforded by the two excipient groups are additive, resulting in up to a complete protection against lyophilization-induced inactivation when representatives of the two are present together. (+info)Replacement of the proximal heme thiolate ligand in chloroperoxidase with a histidine residue. (3/107)
Chloroperoxidase is a versatile heme enzyme which can cross over the catalytic boundaries of other oxidative hemoproteins and perform multiple functions. Chloroperoxidase, in addition to catalyzing classical peroxidative reactions, also acts as a P450 cytochrome and a potent catalase. The multiple functions of chloroperoxidase must be derived from its unique active site structure. Chloroperoxidase possesses a proximal cysteine thiolate heme iron ligand analogous to the P450 cytochromes; however, unlike the P450 enzymes, chloroperoxidase possesses a very polar environment distal to its heme prosthetic group and contains a glutamic acid residue in close proximity to the heme iron. The presence of a thiolate ligand in chloroperoxidase has long been thought to play an essential role in its chlorination and epoxidation activities; however, the research reported in this paper proves that hypothesis to be invalid. To explore the role of Cys-29, the amino acid residue supplying the thiolate ligand in chloroperoxidase, Cys-29 has been replaced with a histidine residue. Mutant clones of the chloroperoxidase genome have been expressed in a Caldariomyces fumago expression system by using gene replacement rather than gene insertion technology. C. fumago produces wild-type chloroperoxidase, thus requiring gene replacement of the wild type by the mutant gene. To the best of our knowledge, this is the first time that gene replacement has been reported for this type of fungus. The recombinant histidine mutants retain most of their chlorination, peroxidation, epoxidation, and catalase activities. These results downplay the importance of a thiolate ligand in chloroperoxidase and suggest that the distal environment of the heme active site plays the major role in maintaining the diverse activities of this enzyme. (+info)Peroxidase and phosphatase activity of active-site mutants of vanadium chloroperoxidase from the fungus Curvularia inaequalis. Implications for the catalytic mechanisms. (4/107)
Mutation studies were performed on active-site residues of vanadium chloroperoxidase from the fungus Curvularia inaequalis, an enzyme which exhibits both haloperoxidase and phosphatase activity and is related to glucose-6-phosphatase. The effects of mutation to alanine on haloperoxidase activity were studied for the proposed catalytic residue His-404 and for residue Asp-292, which is located close to the vanadate cofactor. The mutants were strongly impaired in their ability to oxidize chloride but still oxidized bromide, although they inactivate during turnover. The effects on the optical absorption spectrum of vanadium chloroperoxidase indicate that mutant H404A has a reduced affinity for the cofactor, whereas this affinity is unchanged in mutant D292A. The effect on the phosphatase activity of the apoenzyme was investigated for six mutants of putative catalytic residues. Effects of mutation of His-496, Arg-490, Arg-360, Lys-353, and His-404 to alanine are in line with their proposed roles in nucleophilic attack, transition-state stabilization, and leaving-group protonation. Asp-292 is excluded as the group that protonates the leaving group. A model based on the mutagenesis studies is presented and may serve as a template for glucose-6-phosphatase and other related phosphatases. Hydrolysis of a phospho-histidine intermediate is the rate-determining step in the phosphatase activity of apochloroperoxidase, as shown by burst kinetics. (+info)X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate. (5/107)
The structure of Escherichia blattae non-specific acid phosphatase (EB-NSAP) has been determined at 1.9 A resolution with a bound sulfate marking the phosphate-binding site. The enzyme is a 150 kDa homohexamer. EB-NSAP shares a conserved sequence motif not only with several lipid phosphatases and the mammalian glucose-6-phosphatases, but also with the vanadium-containing chloroperoxidase (CPO) of Curvularia inaequalis. Comparison of the crystal structures of EB-NSAP and CPO reveals striking similarity in the active site structures. In addition, the topology of the EB-NSAP core shows considerable similarity to the fold of the active site containing part of the monomeric 67 kDa CPO, despite the lack of further sequence identity. These two enzymes are apparently related by divergent evolution. We have also determined the crystal structure of EB-NSAP complexed with the transition-state analog molybdate. Structural comparison of the native enzyme and the enzyme-molybdate complex reveals that the side-chain of His150, a putative catalytic residue, moves toward the molybdate so that it forms a hydrogen bond with the metal oxyanion when the molybdenum forms a covalent bond with NE2 of His189. (+info)Prochiral selectivity in H(2)O(2)-promoted oxidation of arylalkanols catalysed by chloroperoxidase. The role of the interactions between the OH group and the amino-acid residues in the enzyme active site. (6/107)
The H(2)O(2)-promoted oxidations of (R)-[alpha-(2)H(1)]-and (S)-[alpha-(2)H(1)]-arylalkanols catalysed by chloroperoxidase (CPO) from Caldariomyces fumago have been investigated. It has been found that with (R)-[alpha-(2)H(1)]-alcohols, the oxidation involves almost exclusively the cleavage of the C-H bond, whereas in the case of the oxidation of (S)-[alpha-(2)H(1)]-alcohols, the C-D bond is preferentially broken. These results clearly indicate that the reactions of corresponding undeuterated arylalkanols are characterized by a high prochiral selectivity, involving the cleavage of the pro-S C-H bond. This prochiral selectivity is poorly influenced by the electronic effect of ring substituents, whereas it decreases with the length of the carbon lateral chain, in the order: benzyl alcohol > 2-phenylethanol > 3-phenylpropanol. Molecular binding studies showed that the main factor directing the docking of the substrate in such a specific orientation in the enzyme active site is the interaction between the alcoholic OH group and the residue Glu183. This interaction is likely to drive both the stereochemistry and the regiochemistry of these reactions. A bifurcated hydrogen bond involving the OH group, the carboxylate oxygen of Glu183 and the oxoferryl oxygen might also be operating. (+info)Expression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme. (7/107)
The Caldariomyces fumago chloroperoxidase was successfully expressed in Aspergillus niger. The recombinant enzyme was produced in the culture medium as an active protein and could be purified by a three-step purification procedure. The catalytic behavior of recombinant chloroperoxidase (rCPO) was studied and compared with that of native CPO. The specific chlorination activity (47 units/nmol) of rCPO and its pH optimum (pH 2.75) were very similar to those of native CPO. rCPO catalyzes the oxidation of various substrates in comparable yields and selectivities to native CPO. Indole was oxidized to 2-oxindole with 99% selectivity and thioanisole to the corresponding R-sulfoxide (enantiomeric excess >98%). Incorporation of (18)O from labeled H(2)18O(2) into the oxidized products was 100% in both cases. (+info)Roles of water in heme peroxidase and catalase mechanisms. (8/107)
A water molecule is coproduced with the Compound I intermediate in the reactions of native heme peroxidases and catalases with hydrogen peroxide. As a result of water release/rebinding from/to the coproduct formation site the Compound I intermediate may exist in two forms: a "wet" form, Compound I(H(2)O), in which a water molecule is present at or near the site of coproduct water formation, and Compound I, in which the coproduct water formation site is "dry." It is postulated that the absence or presence of a water molecule at this site provides the structural basis for a redox pathway switching mechanism, such that the transition states for 2-electron equivalent reduction of Compound I intermediates are accessible in the dry form, but that in the wet form only 1-electron equivalent processes are possible, unless release of water can be stimulated. This concept provides the basis of a general mechanism in which the classical functional distinction between catalases and peroxidases, as well as the more complex behavior observed in halide oxidation and halogenation reactions, appear as particular cases in which variations in the degree of retention of water at the coproduct formation site influence Compound I reactivity. (+info)
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Chloride peroxidase
... (EC 1.11.1.10) is a family of enzymes that catalyzes the chlorination of organic compounds. This enzyme ... Hager LP, Hollenberg PF, Rand-Meir T, Chiang R, Doubek D (1975). "Chemistry of peroxidase intermediates". Ann. N. Y. Acad. Sci ... This enzyme belongs to the family of oxidoreductases, specifically those acting on a peroxide as acceptors (peroxidases). The ... Poulos TL, Sundaramoorthy M, Terner J (1995). "The crystal structure of chloroperoxidase: a heme peroxidase--cytochrome P450 ...
Haloperoxidase
For example, although eosinophil peroxidase is able to oxidize chloride, it preferentially oxidizes bromide. The mammalian ... Horseradish peroxidase is also capable of oxidizing these substrates, but its heme is not covalently bound and becomes damaged ... Haloperoxidases are peroxidases that are able to mediate the oxidation of halides by hydrogen peroxide. Both halides and ... The Nernst equation shows that hydrogen peroxide can oxidize chloride (E°= 1.36 V), bromide (E°= 1.09 V) and iodide (E°= 0.536 ...
Bromide
Hypobromite is produced via eosinophil peroxidase, an enzyme that can use chloride but preferentially uses bromide. The average ... see lithium chloride). Like lithium carbonate and lithium chloride, it was used as a treatment for bipolar disorder. From 1954 ... of those in blood and are strongly influenced by the body's chloride intake and metabolism. Since bromide is still used in ... its relationship to levels of both chloride and total dissolved solids and the implications for water treatment, Chemosphere, ...
Peroxidase
Ascorbate peroxidase Chloride peroxidase Cytochrome c peroxidase Haloperoxidase Hemoprotein Immunoperoxidase Lactoperoxidase ... Haem-using haem peroxidase and the related animal heme-dependent peroxidases DyP-type peroxidase family Catalase some ... Peroxidases are sometimes used as histological markers. Cytochrome c peroxidase is used as a soluble, easily purified model for ... Peroxidases typically catalyze a reaction of the form: ROOR ′ + 2 e − electron donor + 2 H + → Peroxidase ROH + R ′ OH {\ ...
Bromide peroxidase
For example, eosinophil peroxidase appears to prefer bromide over chloride, yet is not considered a bromoperoxidase because it ... Bromide peroxidase (EC 1.11.1.18, bromoperoxidase, haloperoxidase (ambiguous), eosinophil peroxidase) is a family of enzymes ... Bromide+peroxidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (Articles with short ... Bromo peroxidases of red and brown marine algae (Rhodophyta and Phaeophyta) contain vanadate (vanadium bromoperoxidase). ...
List of MeSH codes (D08)
... chloride peroxidase MeSH D08.811.682.732.380 - cytochrome-c peroxidase MeSH D08.811.682.732.440 - eosinophil peroxidase MeSH ... iodide peroxidase MeSH D08.811.682.732.550 - lactoperoxidase MeSH D08.811.682.732.700 - peroxidase MeSH D08.811.682.810.567 - ... horseradish peroxidase MeSH D08.811.682.732.512.900 - wheat germ agglutinin-horseradish peroxidase conjugate MeSH D08.811. ... D08.811.682.732.500 - glutathione peroxidase MeSH D08.811.682.732.512 - ...
CPO
Chile Charge Point Operator installs and maintains charge stations Chloride peroxidase, an enzyme Classic Produktion Osnabrück ...
Bromine
Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating ... Conversely, the bromide ion is a weaker reducing agent than iodide, but a stronger one than chloride. These similarities led to ... Anhydrous hydrogen bromide is a poor solvent, only able to dissolve small molecular compounds such as nitrosyl chloride and ... For example, in the presence of hydrogen peroxide, H2O2, formed by the eosinophil, and either chloride or bromide ions, ...
Organobromine compound
... eosinophil peroxidase, important for defense against multicellular parasites, uses bromide ion in preference to chloride ion. 5 ... Even though the concentration of bromide is only 0.3% of that for chloride in sea water, organobromine compounds are more ...
Eosinophil peroxidase
EPO can take chloride, bromide and iodide as substrates, as well as the pseudohalide thiocyanate (SCN−). However, the enzyme ... Eosinophil peroxidase is a heme peroxidase, its activities including the oxidation of halide ions to bacteriocidal reactive ... Eosinophil+peroxidase at the US National Library of Medicine Medical Subject Headings (MeSH) Eosinophil peroxidase on InterPro ... Eosinophil peroxidase can be found in the primary (azurophilic) granules of human and mammalian leukocytes. Peroxidase ...
Hypobromite
These cells react the bromide with peroxide to generate hypobromite by the action of eosinophil peroxidase, a haloperoxidase ... enzyme which preferentially uses bromide over chloride for this purpose. Simple bromide salts (such as sodium bromide) are also ... in a similar fashion to the action of peroxidase on bromide in eosinophils. Hypobromite has been proposed to be a reactive ...
Myeloperoxidase
... (MPO) is a peroxidase enzyme that in humans is encoded by the MPO gene on chromosome 17. MPO is most abundantly ... and chloride anion (Cl−) (or hypobromous acid if Br- is present) during the neutrophil's respiratory burst. It requires heme as ... MPO is a member of the XPO subfamily of peroxidases and produces hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) ...
ELISpot
If streptavidin-HRP (streptavidin and horseradish peroxidase conjugate) is used, then using TMB (tetramethylbenzidine) as a ... a mixture of 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium chloride) as a substrate will produce more distinct ...
Thyroid
Iodide then travels from within the cell into the lumen, through the action of pendrin, an iodide-chloride antiporter. In the ... Antibodies against thyroid peroxidase can be found on testing. The inflammation usually resolves without treatment, although ... and the iodine is attached to the active tyrosine units in thyroglobulin by the enzyme thyroid peroxidase. This forms the ...
Chlorite dismutase
... and chloride, Cl−. Chlorite dismutase is a heme-containing protein, but it bears no structural or sequence relationships with ... known peroxidases or other heme proteins and is part of a large family of proteins with more than one biochemical function. ...
Organoselenium chemistry
The chlorides are obtained by chlorination of the selenenyl chloride. Seleniranes are three-membered rings (parent: C2H4Se) ... They occur in some selenoenzymes, such as glutathione peroxidase. Seleninic acids (RSe(O)OH) are analogues of sulfinic acids. ...
List of EC numbers (EC 1)
... peroxidase EC 1.11.1.8: iodide peroxidase EC 1.11.1.9: glutathione peroxidase EC 1.11.1.10: chloride peroxidase EC 1.11.1.11: L ... NADH peroxidase EC 1.11.1.2: NADPH peroxidase EC 1.11.1.3: fatty-acid peroxidase EC 1.11.1.4: Now EC 1.13.11.11 EC 1.13.11.11 ... versatile peroxidase EC 1.11.1.17: glutathione amide-dependent peroxidase EC 1.11.1.18: bromide peroxidase EC 1.11.1.19: dye ... phospholipid-hydroperoxide glutathione peroxidase EC 1.11.1.13: manganese peroxidase EC 1.11.1.14: lignin peroxidase EC 1.11. ...
Heme
... l is one important characteristic of animal peroxidases; plant peroxidases incorporate heme B. Lactoperoxidase and ... giving this enzyme the unique capability of easily oxidizing chloride and bromide ions to hypochlorite and hypobromite. ... In peroxidase reactions, the porphyrin molecule also serves as an electron source, being able to delocalize radical electrons ... Heme m contains the two ester bonds at the heme 1- and 5-methyl groups also present in heme l of other mammalian peroxidases, ...
Lanthanum
Like horseradish peroxidase, lanthanum is used as an electron-dense tracer in molecular biology. Lanthanum-modified bentonite ( ... Lanthanum metal is obtained from its oxide by heating it with ammonium chloride or fluoride and hydrofluoric acid at 300-400 °C ... Cerium-doped lanthanum bromide and lanthanum chloride are the recent inorganic scintillators, which have a combination of high ... Various compounds of lanthanum and other rare-earth elements (oxides, chlorides, etc.) are components of various catalysis, ...
Jeewanu
Bahadur also reported having detected ATPase-like and peroxidase-like activity. Bahadur stated that by using molybdenum as a ... Inorganic substances such as colloidal ferric chloride or molybdenum compounds supposedly acted as cofactors and catalysts. ... colloidal molybdenum oxide or potassium nitrate and ferric chloride under sunlight. It appears that this experimental approach ...
Bis(trifluoromethyl)peroxide
For bis(trifluoromethyl)peroxide this will be the following reaction: Peroxidase + C2F6O2 → 2CF3O− The peroxidase will then ... The deactivation is carried out by adding anhydrous calcium chloride to the mixture. The deactivated mixture is scrubbed with ... These pathways make use of different enzymes, but of the same sort, peroxidases. The phase 1 metabolism of peroxides is an ...
Glucose
Another enzyme, peroxidase, catalyzes a chromogenic reaction (Trinder reaction) of phenol with 4-aminoantipyrine to a purple ... It is also on the list in combination with sodium chloride. The name glucose is derived from Ancient Greek γλεῦκος (gleûkos, " ... Glucose forms a black mass with stannous chloride. In an ammoniacal silver solution, glucose (as well as lactose and dextrin) ... "Dextrose mixture with sodium chloride". Drug Information Portal. U.S. National Library of Medicine. Portals: Chemistry Medicine ...
3,3'-Diaminobenzidine
In research, this reaction is used to stain cells that were prepared with hydrogen peroxidase enzyme, following common ... chloride instead of iron powder or with sodium dithionite in methanol. In its main application, DAB is the precursor to ... One other method uses complexes of injected biocytin with avidin or streptavidin, biotin, and then peroxidase. Hans Schwenecke ... which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable ...
Blue bottle experiment
Second, the horseradish peroxidase utilizes the hydrogen peroxide to oxidize ABTS to its radical cationic form, ABTS+•. As the ... Another formula consists of indigo carmine , ascorbic acid (Vitamin C), sodium bicarbonate, sodium chloride, copper(II) sulfate ... The experiment is performed in a clear glass vial containing two common enzymes (glucose oxidase and horseradish peroxidase), ...
Hypochlorite
Upon heating, hypochlorite degrades to a mixture of chloride, oxygen, and chlorates: 2 ClO− → 2 Cl− + O 2 3 ClO− → 2 Cl− + ClO ... "New and classic families of secreted fungal heme peroxidases". Appl Microbiol Biotechnol. 87 (3): 871-897. doi:10.1007/s00253- ... This enzyme combines the inorganic substrates chloride and hydrogen peroxide to produce the equivalent of Cl+, which replaces a ... Thomas, E. L. (1979). "Myeloperoxidase, hydrogen peroxide, chloride antimicrobial system: Nitrogen-chlorine derivatives of ...
Ebselen
It acts as a mimic of glutathione peroxidase and can also react with peroxynitrite. It is being investigated as a possible ... benzoyl chloride (Route I), by ortho-lithiation of benzanilides followed by oxidative cyclization (Route II) mediated by cupric ... "Ebselen treatment reduces noise induced hearing loss via the mimicry and induction of glutathione peroxidase". Hearing Research ...
Iodide
Most iodide salts are soluble in water, but often less so than the related chlorides and bromides. Iodide, being large, is less ... peroxidase + H2O2 + tyrosine, histidine, lipid, etc. → iodo-compounds + H2O + 2 e− (antioxidants). Iodargyrite-natural, ... One consequence of this is that sodium iodide is highly soluble in acetone, whereas sodium chloride is not. The low solubility ... For comparison, the lighter halides are considerably smaller: bromide (196 pm), chloride (181 pm), and fluoride (133 pm). In ...
Arsenic biochemistry
... dimethylarsenic chloride. Some were employed as chemical warfare agents, especially in World War I. This threat led to many ... that take place which may increase the toxicity of arsenic due to MMeAsIII being a potent inhibitor of glutathione peroxidase, ...
Human nutrition
Chlorine as chloride ions; very common electrolyte; see sodium, below. Magnesium, required for processing ATP and related ... Molybdenum required for xanthine oxidase and related oxidases Selenium required for peroxidase (antioxidant proteins) Zinc ... typically as sodium chloride (salt). Excessive sodium consumption can deplete calcium and magnesium. Sodium has a role in the ...
Bioinorganic chemistry
These systems include peroxidases, catalases, and superoxide dismutases. A complementary metalloprotein to those that react ... The most important ions are sodium, potassium, calcium, magnesium, chloride, phosphate, and bicarbonate. The maintenance of ...
Metabolism
The most important ions are sodium, potassium, calcium, magnesium, chloride, phosphate and the organic ion bicarbonate. The ... damaging oxidants are removed by antioxidant metabolites such as glutathione and enzymes such as catalases and peroxidases. ...
Topical fluoride
It is generally prepared in the form of sodium chloride. Sodium chloride is kept in the saliva after spitting out the mouth ... Effects of local treatment with sodium fluoride mouthrinse on peroxidase and hypothiocyanite saliva levels in adolescent]/". ... while strontium chloride and potassium nitrate are added as anti-sensitive agents for individuals who have teeth sensitivity. ...
List of OMIM disorder codes
NSDHL Chloride diarrhea, congenital, Finnish type; 214700; SLC26A3 Cholestasis, benign recurrent intrahepatic, 2; 605479; ... PRSS7 Eosinophil peroxidase deficiency; 261500; EPX Epidermodysplasia verruciformis; 226400; TMC6 Epidermodysplasia ... ATP2A1 Bronchiectasis with or without elevated sweat chloride 1; 211400; SCNN1B Bronchiectasis with or without elevated sweat ... chloride 2; 613021; SCNN1A Bronchiectasis with or without elevated sweat chloride 3; 613071; SCNN1G Brooke-Spiegler syndrome; ...
Phytoremediation
Salt-tolerant (moderately halophytic) barley and/or sugar beets are commonly used for the extraction of sodium chloride (common ... in plants enzymes such as peroxidases, phenoloxidases, esterases and nitroreductases carry out the same role. In the second ...
Saliva
Banerjee, R. K.; Bose, A. K.; Chakraborty, T. K.; De, S. K.; Datta, A. G. (1985). "Peroxidase-catalysed iodotyrosine formation ... 0.08-0.5 mmol/L magnesium 5-40 mmol/L chloride (lower than plasma) 25 mmol/L bicarbonate (higher than plasma) 1.4-39 mmol/L ... Banerjee, R. K.; Datta, A. G. (1986). "Salivary peroxidases". Mol Cell Biochem. 70 (1): 21-29. doi:10.1007/bf00233801. PMID ... lysozyme and peroxidase. It has not been shown that human licking of wounds disinfects them, but licking is likely to help ...
Selenium
The only stable chloride is selenium monochloride (Se2Cl2), which might be better known as selenium(I) chloride; the ... One family of selenium-bearing molecules (the glutathione peroxidases) destroys peroxide and repairs damaged peroxidized cell ... such as glutathione peroxidases and certain forms of thioredoxin reductase found in animals and some plants (this enzyme occurs ... a dithiol that serves as an electron source for peroxidases and also the important reducing enzyme ribonucleotide reductase ...
Urine test strip
Vegetable peroxidase and bacterial enzymes, including an Escherichia coli peroxidase, may also cause false-positive reactions. ... stannous chloride). On application of a drop of urine and heating over a candle the strip immediately turned black if the urine ... The urine test strip test for blood is based on hemoglobin's pseudo peroxidase activity in catalysing a reaction between ... A second linked reaction, mediated by a peroxidase, catalyses the reaction between the peroxide and a chromogen (a substance ...
Hypospermia
However, in moderate levels, the antioxidant properties of selenium, likely due to an increase in glutathione peroxidase-1 ... gene which encodes for chloride channel protein. This is a condition that is caused by obstruction of either one or both ...
List of ASTM International standards (D6001-7000)
Vinyl Chloride) (PVC) and Chlorinated Poly(Vinyl Chloride) (CPVC) D6264/D6264M - 17 Test Method for Measuring the Damage ... 20 Test Method for Determination of Hydrogen Peroxide and Combined Organic Peroxides in Atmospheric Water Samples by Peroxidase ... Vinyl Chloride) Insulation for Wire and Cable, 90 °C Operation D6097 - 16 Test Method for Relative Resistance to Vented Water- ... Vinyl Chloride)(PVC) Siding D6778 - 20 Classification System and Basis for Specification for Polyoxymethylene Molding and ...
NFAT5
... stimulates the transcription of genes for aldose reductase (AR), the sodium chloride-betaine cotransporter (SLC6A12) the ... 2002). "The characterization and purification of a human transcription factor modulating the glutathione peroxidase gene in ...
Carbon monoxide-releasing molecules
Methylene chloride was the first organic CORM orally administered based on previous reports of carboxyhemoglobin formation via ... peroxidases, soluble guanylate cyclase, nitric oxide synthase) and ineffective erythropoiesis in bone marrow. The enzymatic ...
Mutagen
Other alkylating agents include mustard gas and vinyl chloride. Aromatic amines and amides have been associated with ... is a component of important antioxidant enzymes such as gluthathione peroxidase. Many phytonutrients may counter the effect of ...
Ethylenediaminetetraacetic acid
It serves as a preservative (usually to enhance the action of another preservative such as benzalkonium chloride or thiomersal ... Bhattacharyya, D K; Adak, S; Bandyopadhyay, U; Banerjee, R K (1994-03-01). "Mechanism of inhibition of horseradish peroxidase- ... liver arginase and horseradish peroxidase independently of metal ion chelation. These findings urge the rethinking of the ...
Pendrin
Karniski LP, Aronson PS (September 1985). "Chloride/formate exchange with formic acid recycling: a mechanism of active chloride ... "Thyroid peroxidase: evidence for disease gene exclusion in Pendred's syndrome". Clinical Endocrinology. 44 (4): 441-6. doi: ... This is probably the result of other bicarbonate or chloride transporters in the kidney compensating for any loss of pendrin ... Scott DA, Wang R, Kreman TM, Sheffield VC, Karniski LP (April 1999). "The Pendred syndrome gene encodes a chloride-iodide ...
N,N-Dimethyltryptamine
The two most commonly encountered synthetic routes are through the reaction of indole with oxalyl chloride followed by reaction ... April 2014). "Biosynthesis of N,N-dimethyltryptamine (DMT) in a melanoma cell line and its metabolization by peroxidases". ... in the human melanoma cell line SK-Mel-147 including details on its metabolism by peroxidases. It is assumed that more than ... namely tryptamine and oxalyl chloride. Instead, it is more often extracted from plant sources using a non-polar hydrocarbon ...
Prothioconazole
The chloride within the chloromethyl group is subsequently substituted by 1,2,4-triazole. Finally, to introduce the thioketone ... Studies also suggest that prothioconazole can also interact with and temporarily suppress thyroid peroxidase. This enzyme is ... The Grignard derivative of 2-chlorobenzyl chloride is added across the double bond of 1-chlorocyclopropyl-2-chloro-ethan-1-one ...
Vitamin E
Alpha-tocopherol is a lipid-soluble antioxidant functioning within the glutathione peroxidase pathway, and protecting cell ... using iron in the presence of hydrogen chloride gas as catalyst. The reaction mixture obtained is filtered and extracted with ...
Dioxygen in biological reactions
Glutathione peroxidase and similar enzymes then convert the H2O2 to water and dioxygen. Parts of the immune system of higher ... Manganese is an important cofactor, and calcium and chloride are also required for the reaction to occur. In vertebrates, ...
SCOP 1.73: Structural Classification of Proteins - extended
DeCS
Code System Concept
Johannes Schelvis - Research output - Montclair State University
Peroxidase intermediate bypass causes poor isoniazid activation by the S315G mutant of M. tuberculosis catalase-peroxidase ( ... Impact of distal side water and residue 315 on ligand binding to ferric Mycobacterium tuberculosis catalase - Peroxidase (KatG) ... Analysis of heme structural heterogeneity in Mycobacterium tuberculosis catalase-peroxidase (KatG). Chouchane, S., Girotto, S. ... Resonance Raman spectroscopy of Compound II and its decay in Mycobacterium tuberculosis catalase-peroxidase KatG and its ...
MMTB
Gene locus Report for: burch-a0b0f8
Rat IgG F(ab')2 Peroxidase (012-0304) | Rockland
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative:. 0.01% (w/v) Thimerosal ... RAT IgG F(ab)2 fragment Peroxidase conjugated - 012-0304 Synonyms:. Rat IgG F(ab)2 fragment peroxidase conjugation, Rat IgG F ... Related products to: Rat IgG F(ab)2 Peroxidase rRat IL-1 beta Protein 012-001-301-0002 ... Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab)2 ...
Phenotypic Similarity of Transmissible Mink Encephalopathy in Cattle and L-type Bovine Spongiform Encephalopathy in a Mouse...
Endogenous peroxidase activity was also blocked. A peroxidase-labeled avidin-biotin complex (Vectastain Elite ABC, Vector ... Final detection was achieved with a solution of diaminobenzidine intensified with nickel chloride (DAB-Ni, Zymed) to give black ... Laboratories, Burlingame, CA, USA) and a solution of diaminobenzidine intensified with nickel chloride (DAB-Ni, Zymed, ...
11+ Important Anion Example You Need To Know - Lambda Geeks
In Eosinophil granulocyte is a peroxidase enzyme where bromide is more acceptable over chloride. ... Chloride (Cl-). Chloride is the simple anion of chlorine. Due to the reduction of Chlorine, we get chloride ions. ... The process is called the chloride shift.. A heme protein Myeloperoxidase is converted chloride ion converted to hypochlorous ... Chloride ions in blood plasma enter the RBC in order to maintain the electrical balance as well as acid-base balance RBC. ...
Pesquisa | Portal Regional da BVS
Amidrazone modified acrylic fabric activated with cyanuric chloride: A novel and efficient support for horseradish peroxidase ... activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The ... However, increasing the chloride content of water significantly improved the efficiency of treatment due to the enhanced ... Immobilization of horseradish peroxidase on cationic microporous starch: Physico-bio-chemical characterization and removal of ...
Subject: Glycine max and citrates / Subject term: citrates - PubAg Search Results
Glycine max; acid soils; aluminum; antagonists; ascorbate peroxidase; biosynthesis; calcium; calcium channels; calcium chloride ... magnesium chloride; protons; root growth; roots; secretion; soil pH; soil solution; soybeans; toxicity. Abstract:. ... ... peroxidase; root growth; roots; salicylic acid; secretion; signal transduction; soybeans; stress response; superoxide dismutase ...
Eosinophil peroxidase - Rediff Pages : 1107658
Follow Eosinophil peroxidase to get latest updates from Eosinophil peroxidase ... Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating ... Eosinophil peroxidase is a haloperoxidase enzyme that in humans is encoded by the EPX gene. The enzyme is a heterodimeric 71-77 ... Eosinophil peroxidase is also partly responsible for tissue remodeling.. Role in pathology The oxidizing compounds produced by ...
Antioxidant effect of Arabic gum against mercuric chloride-induced nephrotoxicity
A single dose of mercuric chloride (5 mg/kg intraperitoneal injection) induced renal toxicity, manifested biochemically by a ... peroxidase and catalase in kidney homogenate. GSH content and lipid peroxidation (malondialdehyde pro-duction) in the kidney ... Effect of lycopene on nephrotoxicity induced by mercuric chloride in rats. Basic Clin Pharmacol Toxicol. 2007;100(6):398-402. ... Mercuric chloride effects on rat renal redox enzymes activities: SOD protection. Free Radic Biol Med. 1995;18(1):61-66. ...
Diabetes | Fucoidan | Antioxidant Enzymes | Free Radicals | Superoxide Dismutase | Catalase | Glutathione Peroxidase |...
... by the barium chloride method [24,25]. The fucose and sulfate contents of fucoidan showed were; 70.5±2.1 % and 45.0±1.20 ... The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked ... Diabetes, fucoidan, antioxidant enzymes, free radicals, superoxide dismutase, catalase, glutathione peroxidase, glutathione S- ... Peroxidase (GPx), Glutathione S-Transferase (GST) and increased activity of lipid hydroperoxidase [19]. Therefore, it has been ...
NIOSHTIC-2 Search Results - Full View
Clarifying Enzyme Exfoliating Scrub - KERSTIN FLORIAN
Facet-search | Sigma-Aldrich
Measuring Peroxidase in Milk. Application Note: Peroxidase in milk - Visual determination after conversion of a specific ... In a glass bottle mix 50 g of naturally moist sample, free from coarse stones, with 100 ml of the calcium chloride solution ... Analytical Method: Chloride in building materials. Photometric determination using the iron(III)/mercury thiocyanate method ... Photometric determination using the indophenol blue method after extraction with calcium chloride solution ...
RUNG - Iskanje
Horseradish Peroxidase | Harvard Catalyst Profiles | Harvard Catalyst
Chloride Peroxidase. *Cytochrome-c Peroxidase. *Eosinophil Peroxidase. *Glutathione Peroxidase. *Horseradish Peroxidase. * ... Peroxidase-immobilized porous silica particles for in situ formation of peroxidase-free hydrogels with attenuated immune ... "Horseradish Peroxidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles ...
Chicken IgG F(ab')2 Antibody Peroxidase Conjugated (603-4304) | Rockland
Antibody Peroxidase Conjugated - 603-4304 for ELISA. Reacts with Chicken. Order online now! ... 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative:. 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium ... Related products to: Chicken IgG F(ab)2 Antibody Peroxidase Conjugated. Chicken IgM (mu chain) Antibody Biotin Conjugated 603- ... Anti-Chicken IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for use in immunoelectrophoresis, ...
JC-1 (chloride salt) - Biotium
Peroxidase, Galactosidase, Phosphatase, & Other Substrates. *Protease Substrates. *View all in Enzyme Substrates ... JC-1 (chloride salt) JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial ... Biotium developed JC-1 chloride salt for applications where iodide ion is not desired. JC-1 chloride may also have the ... JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains ...
MMTB
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Water/Aqua/Eau Isododecane Cyclopentasiloxane Polyquaternium-16 Panthenyl Hydroxypropyl Steardimonium Chloride Helianthus ... Seed Peroxidase Saccharomyces/Copper Ferment Daucus Carota Sativa (Carrot) Root Extract Ganoderma Lucidum (Mushroom) Tyrosinase ... Cetyldimethylamine Hydrolyzed Hempseedate Cetrimonium Chloride Phenyl Trimethicone Laureth-4 Polysorbate 20 Phenoxyethanol ...
IMSEAR at SEARO: Influence of industrial pollutants on thyroid function in Channa punctatus (Bloch).
Neuroprotective effects of curdione against focal cerebral ischemia re | NDT
The malonaldehyde (MDA) levels and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH ... The infarct size of animals was determined by the 2,3,5-triphenyltetrazolium chloride staining, and pathological brain damage ... The sections were stained with 2,3,5-triphenyltetrazolium chloride (TTC) at 37°C for 10 min and then fixed with 4% ... The infarct size of animals was determined by the 2,3,5-triphenyltetrazolium chloride staining, and pathological brain damage ...
Introduction
In contrast, eosinophils, which possess eosinophil peroxidase, demonstrated peroxidase staining [30]. .. Animals were housed ... which oxidizes chloride by a single 2-electron transfer to produce the hypochlorous acid (HOCl). Both oxidants - reactive MPO ... Reactive intermediates can be also generated by other heme-peroxidases such as eosinophil peroxidase, lactoperoxidase, thyroid ... Peroxidases.. Chem Biol Interact. 129. 113. 139. 11154738. 26. Scott. CD. Povitsky. A. Dateo. C. Gokcen. T. Willis. PA. 2003. ...
Pakistan Journal of Botany
... peroxidase and ascorbate peroxidase. As the concentration of sodium chloride increases the enzyme activity also increases and ... Ammonium chloride (1%) as inorganic nitrogen source, nutrient broth (0.8%) as organic nitrogen source and starch (0.8%) as ... In conclusion, application of AMF could alleviate the negative effect of sodium chloride and can be used as a suitable approach ... The present study was conducted to assess the effect of different concentrations of sodium chloride (NaCl) in presence and ...
Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness | BMC...
Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and ... Sodium chloride (1 M) and GnCl (200 mM) were added to the dialysed fractions, and these latter were subjected to nickel ... The peroxidase from horseradish roots (Armoracia rusticana; HRP, isoform C) is the most widely studied peroxidase, due mainly ... A single peroxidase stock solution was placed for 10 min at progressively increasing temperatures, at which time aliquots were ...
Download Adventure Guide To Puerto Rico, 3Rd Edition (Hunter Travel Guides) 1996
CatalaseHorseradishGlutathione peroxidase and glutathioneSuperoxideIodideHemeBromideCadmiumHypochlorous acidConjugateCalciumConjugationIonsChlorineEnzymeEosinophilSodiumSerumAntibodyRatsActivityCellsFreeTotalHorseradishSuperoxide DismutaseGlutathione peroxidase activityHydrogenIntracellular channel proteinProteinPotassiumMagnesiumSeleniumCholine chloridePresence of chlorideCadmium chlorideLipidLithium chlorideAntibodiesCobaltAlkaline phosphataseSodium azideOverexpressionConcentrationAnhydrousBufferMercuricH2O2PhysiologicalAssayStimulatesPeroxideAnalyticalIodineComplexSalt
Catalase4
- In addition, reduced glutathione, glutathione peroxidase, and catalase enzymes in renal tissues were significantly decreased. (1library.net)
- The other tested antioxidant enzymes such as catalase, glutathione peroxidase and glutathione S transferase also showed marked regain in a dose-dependent manner upon administration of fucoidan. (ijpsonline.com)
- The malonaldehyde (MDA) levels and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) were detected by enzyme-linked immunosorbent assay. (dovepress.com)
- The activities of the antioxidant enzymes, catechol-peroxidase (POX) and catalase (CAT) increased progressively with the increase in CdCl2 and NaCl concentrations. (ujconline.net)
Horseradish7
- The data depicted that the immobilization of horseradish peroxidase (HRP) on CMPS revealed highest immobilization efficiency (86%) at 100â ¯mg of CMPS at pHâ ¯=â ¯6.0 and 100â ¯units of enzyme. (bvsalud.org)
- Horseradish Peroxidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- This graph shows the total number of publications written about "Horseradish Peroxidase" by people in Harvard Catalyst Profiles by year, and whether "Horseradish Peroxidase" was a major or minor topic of these publication. (harvard.edu)
- Below are the most recent publications written about "Horseradish Peroxidase" by people in Profiles. (harvard.edu)
- Detecting Horseradish Peroxidase-Labeled Cells. (harvard.edu)
- Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. (biomedcentral.com)
- The Onuf nucleus shows changes after injection of horseradish peroxidase into either the pudendal or the pelvic nerve. (medscape.com)
Glutathione peroxidase and glutathione1
- After a 20 to 44 hour incubation period in less than 0.05 millimoles of the chlorides of Cd(2+), Zn(2+) and Hg(2+), there was no increase of malonaldehyde (542789) or in the activity of glutathione peroxidase and glutathione reductase. (cdc.gov)
Superoxide4
- This damage was caused by decreased levels of the testicular antioxidant glutathione (GSH) and activity levels of the testicular antioxidants superoxide dismutase (SOD) and GSH peroxidase (GSH-Px), as well as increased lipid peroxidation and hydrogen peroxide production in the testes. (naturalsociety.com)
- Proline, lipid peroxidase (LP), superoxide dismutase (SOD) and glutathione (GSH) were determined following standard protocols. (springeropen.com)
- In the cadmium chloride group, testicular tissue glutathione (GSH) and 8-OHdG levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and serum testosterone levels were significantly decreased compared to other groups. (bioimmersion.com)
- Peroxidase (POD) activity and superoxide dismutase (SOD) also decreased, whereas the H 2 O 2 and MDA content increased. (ashs.org)
Iodide2
- A 30 day exposure of C. punctatus to sublethal levels of phenol, ammonia, mercuric chloride, cadmium chloride and a mixture of the four resulted in an overall activation of guaiacol peroxidase and depression of iodide peroxidase (IPOD) activity and blood T4 titre. (who.int)
- Iodide and chloride are fairly inert. (myhdiet.com)
Heme3
- A heme protein Myeloperoxidase is converted chloride ion converted to hypochlorous acid at a plasma concentration of halide. (lambdageeks.com)
- In heme enzymes belonging to the peroxidase-cyclooxygenase superfamily the proximal histidine is in close interaction with a fully conserved asparagine. (rcsb.org)
- Moreover, comprehensive analysis of published lactoperoxidase structures suggest that the described proximal heme architecture is a general structural feature of animal heme peroxidases. (rcsb.org)
Bromide3
- In Eosinophil granulocyte is a peroxidase enzyme where bromide is more acceptable over chloride. (lambdageeks.com)
- This enzyme prefers bromide over chloride as a substrate, converting it to toxic hypobromite. (rediff.com)
- Eosinophil peroxidase is a haloperoxidase that preferentially uses bromide over chloride for this purpose, generating hypobromite ( hypobromous acid ). (rediff.com)
Cadmium5
- The researchers orally administered 4 mg/kg of body weight of cadmium chloride to male mice. (naturalsociety.com)
- Background: The present study aims to investigate the effects of calcium fructoborate on testicular DNA damage and testicular tissue biochemical markers and serum testosterone levels after cadmium chloride administration. (bioimmersion.com)
- Control group (No chemicals applied), calcium fructoborate (100 mg) group, Cadmium chloride (200 mg/L) + calcium fructoborate (100 mg), Cadmium chloride (200 mg/L) group. (bioimmersion.com)
- Results: While 8-hydroxy-2-deoxyguanosine (8-OHdG) expression was moderate in the cadmium chloride + calcium fructoborate group, the expression in the cadmium chloride group was severe. (bioimmersion.com)
- Investigation of the Effects of Calcium Fructoborate on Testicular Structure in Rats within the Framework of Biochemical Parameters, Testosterone Hormone and DNA Damage in Cadmium Chloride Induced Toxicity. (bioimmersion.com)
Hypochlorous acid1
- It completely lost its ability to oxidize chloride to hypochlorous acid, which is a characteristic feature of MPO and essential for its role in host defense. (rcsb.org)
Conjugate1
- Anti-Chicken IgG F(ab')2 Peroxidase conjugate has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. (rockland.com)
Calcium2
- In a glass bottle mix 50 g of naturally moist sample, free from coarse stones, with 100 ml of the calcium chloride solution 0.025 mol/l. (sigmaaldrich.com)
- The raw material for processing was prepared through normal proceedings such as cleaning in running water, cut in halves and pre-freezing treatments by using sucrose, calcium chloride (CaCl 2 ), pectin and heat treatment. (usp.br)
Conjugation1
- This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by Peroxidase conjugation. (rockland.com)
Ions2
- Due to the reduction of Chlorine, we get chloride ions. (lambdageeks.com)
- Chloride ions in blood plasma enter the RBC in order to maintain the electrical balance as well as acid-base balance RBC. (lambdageeks.com)
Chlorine1
- Chloride is the simple anion of chlorine. (lambdageeks.com)
Enzyme6
- An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. (bvsalud.org)
- Rat IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. (rockland.com)
- Chloride ion has greater importance to form hydrochloric acid in the human stomach which is maintaining the acidic environment in the enzyme. (lambdageeks.com)
- Eosinophil peroxidase is a haloperoxidase enzyme that in humans is encoded by the EPX gene . (rediff.com)
- The enzyme is a heterodimeric 71-77 kD peroxidase consisting of a heavier glycosylated chain and a lighter nonglycosylated chain. (rediff.com)
- Selenium is important for the activity of the antioxidant enzyme glutathione peroxidase, but it is also important for preventing blood coagulation reactions. (myhdiet.com)
Eosinophil3
- to get instant updates about 'Eosinophil Peroxidase' on your MyPage . (rediff.com)
- Eosinophil peroxidase is also partly responsible for tissue remodeling. (rediff.com)
- The oxidizing compounds produced by eosinophil peroxidase have been implicated in the inflammatory pathology of several disease states, including asthma. (rediff.com)
Sodium1
- Salts of sodium chloride (NaCl) and sodium sulfate (Na 2 SO 4 ) at 5, 10 and 15 dS/m concentrations were used for this study. (springeropen.com)
Serum4
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. (rockland.com)
- A single dose of mercuric chloride (5 mg/kg intraperitoneal injection) induced renal toxicity, manifested biochemically by a significant increase in serum creatinine, blood urea nitrogen, thiobarbituric acid reactive substances, and total nitrate/nitrite production in kidney tissues. (1library.net)
- The antigen is prepared from a modified Venereal Disease Research Laboratory (VDRL) antigen suspension containing choline chloride to eliminate the need to heat-inactivate serum, ethylene-diamine-tetra-acetic acid (EDTA) to enhance the stability of the suspension, and finely divided charcoal particles as a visualizing agent. (cdc.gov)
- More than 50% of these patients have high serum antithyroid peroxidase antibody levels, and nearly 50% have overt hypothyroidism. (medscape.com)
Antibody2
- Rat IgG F(ab')2 Fragment Peroxidase Conjugated reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. (rockland.com)
- Biotinylated anti-human IgG labeled with strepavidin-peroxidase is used to detect the patient's antibody. (cdc.gov)
Rats1
- Pretreatment of rats with AG (7.5 g/kg/day per oral administration), starting 5 days before mercuric chloride injection and continuing through the experimental period, resulted in a complete reversal of Hg-induced increase in creatinine, blood urea nitrogen, thiobarbituric acid reactive substances, and total nitrate/nitrite to control values. (1library.net)
Activity3
Cells2
- JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. (biotium.com)
- Phospholipid hydroperoxide glutathione peroxidase plays a role in protecting cancer cells from docosahexaenoic acid-induced cytotoxicity. (ouhsc.edu)
Free1
- Peroxidase-immobilized porous silica particles for in situ formation of peroxidase-free hydrogels with attenuated immune responses. (harvard.edu)
Total1
- Foram realizadas medidas objetivas de textura, cor, suculência, teor de sólidos solúveis, teor de vitamina C, pH, acidez total titulável, perda de suco por exsudação e atividade da polifenoloxidase e peroxidase. (usp.br)
Horseradish9
- The biotinylated peptide is able to interact with streptavidin-horseradish peroxidase (SA-HRP), which catalyzes the substrate solution composed of 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxidase to produce a blue solution. (medscape.com)
- Novus Biologicals, Oakville, ON, Canada) in preventing solution right away at 4?C. After cleaning with TBST, rabbit horseradish peroxidase-conjugated supplementary antibodies (1? (cenicriviroc.info)
- In this study, we report the preparation, catalytic activity and stability of a hybrid nanoflower (hNF) formed from horseradish peroxidase (HRP) enzyme and copper ions (Cu2+). (erciyes.edu.tr)
- Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. (agrisera.com)
- Peroxidase is widely distributed in the plant kingdom, and its content is high in horseradish , so it is often extracted from horseradish. (sdlookchem.com)
- Membrane was probed with a proper principal antibody and a second antibody conjugated to horseradish peroxidase. (epf2013.org)
- Antibodies were affinity purified and conjugated with horseradish peroxidase (HRP). (deltabiolabs.com)
- Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase. (abcam.cn)
- Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons). (abcam.co.jp)
Superoxide Dismutase7
- Among the various parameters investigated, germination, vigour, total protein content, dehydrogenase, amylase, superoxide dismutase (SOD) and peroxidase activity were found to show a decreasing trend, whereas electrical conductivity (EC) increased during storage irrespective of drying methods. (academicjournals.org)
- In addition, high-density lipoprotein, also known as the "good cholesterol," markedly went up, as well as the number of antioxidant enzymes present in the liver, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). (cures.news)
- The results showed that the effect of salinity stress and foliar application was significant on the leaf yield, the chlorophyll pigments rate (a, b and total), carotenoid, protein content, antioxidant activity catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) and the steviol glycosides composition. (sid.ir)
- increased Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST), urea, and creatinine, as well as malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx). (biomedcentral.com)
- Bacopa monniera exerted antioxidant activity on rat brain frontal cortical, striatal and hippocampal regions by altering superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels. (krishnaherbals.com)
- Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. (notulaebotanicae.ro)
- Alleviates feelings of anxiety and can help to protect the brain - relaxes the inner muscles - has an antioxidant effect: the withanolides increase the value of superoxide dismutase and glutathione peroxidase, two important antioxidants of the organism. (asai.at)
Glutathione peroxidase activity2
Hydrogen3
- Hydrogen peroxide is used to quench endogenous peroxidase in immunohistochemical staining. (nih.gov)
- According to the catalytic properties of Peroxidase, hydrogen peroxide (H2O2) is generally used as one of the HRP substrates in ELISA. (sdlookchem.com)
- The purpose of MPO is to convert hydrogen peroxide into hypochlorite by combining with a chloride anion. (wardroundstuff.com)
Intracellular channel protein1
- This gene encodes a chloride intracellular channel protein. (innatedb.com)
Protein2
- Protein expression Chelerythrine Chloride cell signaling was also quantified by western blot analyses (Supplementary Physique 1B and C) and reduction of the enzymatic activity of MT1-MMP was confirmed by measuring the conversion of proMMP-2 to MMP-2 by zymography (Supplementary Physique 1D and E). A third cell collection was generated with the vacant plasmid as a negative control (mock). (cenicriviroc.info)
- 16. The method of claim 15, wherein symptoms associated with diabetes or with the onset of diabetes are treated by lowering the blood levels of one or more of fasting glucose, glycosylated hemoglobin, monocyte chemotactic protein-1, intracellular adhesion molecule, and red blood cell lipid peroxidase. (freepatentsonline.com)
Potassium1
- Rats supplemented with a VCO diet had modulated levels of creatinine, sodium (Na+), potassium (K+), chloride (Cl? (cures.news)
Magnesium1
- Magnesium chloride pharmacopoeia quality, Ph.Eur. (asai.at)
Selenium4
- This research was aimed to investigate the foliar application of selenium, boron and iron on leaf yield, the photosynthetic pigments rate, antioxidant enzymes activity and the steviol glycosides content under sodium chloride stress conditions. (sid.ir)
- The salinity stress levels were used by sodium chloride at four levels: control (less than 10), 30, 60 and 90 mM, and the foliar application of iron, boron, and selenium was applied. (sid.ir)
- Both the experimental rat groups (Ovx and Ovx-Cd) were gavaged orally with a 2 ml solution of the dietary supplementation (selenium chloride SeCl) individually or in combination with CdCl at a dose of6 mg/kg.B.W, resulting recovery effects for blood parameters and antioxidant enzymatic activities were almost restored to control level, except for peroxidase activity that was respond differently in both type of treatment. (mosuljournals.com)
- SELENIUM is part of glutathione peroxidase which protects cell components from oxidative damage due to peroxides produced in cellular metabolism. (nih.gov)
Choline chloride1
- The antigen is prepared from a modified Venereal Disease Research Laboratory (VDRL) antigen suspension containing choline chloride to eliminate the need to heat-inactivate serum, ethylene-diamine-tetra-acetic acid (EDTA) to enhance the stability of the suspension, and finely divided charcoal particles as a visualizing agent. (cdc.gov)
Presence of chloride3
- The concrete that coated the reinforced steel could not protect the steel from long exposure to oxygen in the air and presence of chloride in the sea water that will corrode the reinforced steel. (jcse.org)
- The common occurrence in reinforced steel is that they are going to corrode or rust overtime especially in sea water where there are presence of chloride ions and the conductivity of water is high which will increase the rate of corrosion.3,4 When corrosion occurred, the reinforced steel will be slowly eaten away and become thinner each day making it looks orange and rough. (jcse.org)
- Under these conditions, 2O2 in the presence of chloride into hypochlorite, which may act directly on the the averaged concentration, [H2O2], can be written as pathogen but also has been implicated in the pathogenesis associated with the acute respiratory distress syndrome (5). (nih.gov)
Cadmium chloride3
- The present investigation was aimed the possibility protective effect of Selenium chloride against oxidative stress during Ovariectomy and Ovariectomy - cadmium chloride exposure, (25) female rats Albino Wister type weighting (250- 300g). (mosuljournals.com)
- Comparative Hepatotoxicity of Inhaled Cadmium Chloride and Cadmium Oxide (Journal Version). (epa.gov)
- Comparative Study of the Effects of Inhaled Cadmium Chloride and Cadmium Oxide: Pulmonary Response. (epa.gov)
Lipid1
- The level of lipid peroxidation was not changed by increasing doses of mercuric chloride, and hence was not a primary toxic mechanism in acute nephrotoxicity induced by mercuric chloride. (nih.gov)
Lithium chloride5
- The effects of four drying methods using silica gel, saturated salt solution of lithium chloride, concentrated sulphuric acid and dryer drying on different physiological and biochemical characteristics of genotypes CSH 16 and CSV 18 of sorghum ( Sorghum bicolor (L.) Moench) were investigated. (academicjournals.org)
- Faster drying rate was observed in acid and silica gel while dryer and saturated salt solution of lithium chloride exhibited slow drying rate. (academicjournals.org)
- Results obtained by silica gel and lithium chloride salt solution drying were comparable with those obtained by seed dryer. (academicjournals.org)
- With an objective of developing efficient, alternative and energy efficient drying methods to reduce seed moisture content without compromising seed health and quality, the present investigation was executed to elucidate the effects of silica gel, saturated salt solution of lithium chloride, concentrated sulphuric acid and dryer drying on the different physiological and biochemical seed characteristics of sorghum genotypes CSH 16 and CSV 18. (academicjournals.org)
- For slow drying seeds were placed in glass desiccators containing saturated salt solution of lithium chloride and seed dryer maintained at 15% R H and temperature at 15°C. (academicjournals.org)
Antibodies1
- In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. (biomeda.com)
Cobalt3
- Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining. (fishersci.co.uk)
- Despite this, our data also indicate that AST could attenuate cobalt chloride-evoked COX-2 activation, while such effect on COX-2 and its downstream target VEGF was intensified when indomethacin was concurrently treated. (biomedcentral.com)
- The use of cobalt salts, as e.g. cobalt chloride and cobalt sulfate is explicitly banned in cosmetic products. (dermaviduals.de)
Alkaline phosphatase1
- Hematoxylin, generally without eosin, is useful as a counterstain for many immunohistochemical or hybridization procedures that use colorimetric substrates (such as alkaline phosphatase or peroxidase). (slideshare.net)
Sodium azide1
- 50 mM Sodium Bicarbonate-Borate, 0.9% Sodium Chloride pH 8.1, 0.05% sodium azide. (biomeda.com)
Overexpression2
- Likewise, the overexpression of glutathione peroxidase (GPx) protects cells from Cr(VI) [86]. (globaltechbiz.com)
- 7. Glutathione peroxidase 2 overexpression promotes malignant progression and cisplatin resistance of KRAS‑mutated lung cancer cells. (nih.gov)
Concentration1
- One common adulterant, sodium chloride, always produces a specific gravity greater than 1.035 if added at a concentration necessary to produce a false-negative result. (aacc.org)
Anhydrous1
- The reduction of anhydrous Iron chloride (FeCl 3 ) in presence of sodium borohydride and water soluble polyelectrolyte (polydiallyldimethyl ammonium chloride, PDADMAC) produces black precipitates. (biomedcentral.com)
Buffer2
- SDS-PAGE and Traditional western Blot Cells had been lysed in radioimmune precipitation assay buffer formulated with 50 mm Tris-HCl, pH 8.0, with 150 mm sodium chloride, 1.0% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate. (epf2013.org)
- Each vial contains 200 g in 0.2ml of affinity purified anti-Mouse IgG, in a buffer containing 50mM HEPES pH 7.0, 0.1 M soduim chloride, 0.2% BSA and 0.01 % Thimerosal. (deltabiolabs.com)
Mercuric2
H2O21
- Here, we show that HOCl generated by the MPO-H2O2-chloride system inactivates TIMP-1 by oxidizing its N-terminal cysteine. (nih.gov)
Physiological1
- In that sense, human being blood is the most complex biological matrix that better resembles the physiological Bethanechol chloride environment. (thetechnoant.info)
Assay1
- This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system. (celltechnology.com)
Stimulates1
- cAMP stimulates cyst formation by promoting chloride-driven fluid secretion. (asnjournals.org)
Peroxide1
- Peroxidases would alter peroxide-mediated signaling, but could also work by stopping HO? (globaltechbiz.com)
Analytical1
- Sodium chloride, meets analytical specification of Ph. (sigmaaldrich.com)
Iodine1
- Peroxidases can oxidize iodide into iodine which is the key substance for the iodine-containing thyroxines of the thyroid gland. (dermaviduals.de)
Complex1
- Possible, they for avidin-Biotin-Peroxidase Complex steroids relieve pressure on nerves and nerve roots. (wahoowebsite.com)
Salt1
- This recent work is motivated by the growing chloride salt) and monitoring distribution in a number of interest in developing MRI techniques that are sensitive to tissues [24, 25]. (nih.gov)