A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.
The trihydrate sodium salt of acetic acid, which is used as a source of sodium ions in solutions for dialysis and as a systemic and urinary alkalizer, diuretic, and expectorant.
A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
A group of chemical elements that are needed in minute quantities for the proper growth, development, and physiology of an organism. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Oxidoreductases that are specific for the reduction of NITRATES.
Membrane proteins whose primary function is to facilitate the transport of negatively charged molecules (anions) across a biological membrane.
An iron-sulfur and MOLYBDENUM containing FLAVOPROTEIN that catalyzes the oxidation of nitrite to nitrate. This enzyme can use either NAD or NADP as cofactors. It is a key enzyme that is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. This enzyme was formerly classified as EC 1.6.6.2.
An NAD-dependent enzyme that catalyzes the oxidation of nitrite to nitrate. It is a FLAVOPROTEIN that contains IRON and MOLYBDENUM and is involved in the first step of nitrate assimilation in PLANTS; FUNGI; and BACTERIA. It was formerly classified as EC 1.6.6.1.
An enzyme that catalyzes the oxidation of nitrite to nitrate. It is a cytochrome protein that contains IRON and MOLYBDENUM.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
A publication issued at stated, more or less regular, intervals.
Membranous cisternae of the CHLOROPLAST containing photosynthetic pigments, reaction centers, and the electron-transport chain. Each thylakoid consists of a flattened sac of membrane enclosing a narrow intra-thylakoid space (Lackie and Dow, Dictionary of Cell Biology, 2nd ed). Individual thylakoids are interconnected and tend to stack to form aggregates called grana. They are found in cyanobacteria and all plants.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Proteins found in any species of algae.
A family of proteins that promote unwinding of RNA during splicing and translation.
A large family of RNA helicases that share a common protein motif with the single letter amino acid sequence D-E-A-D (Asp-Glu-Ala-Asp). In addition to RNA helicase activity, members of the DEAD-box family participate in other aspects of RNA metabolism and regulation of RNA function.
Ribonucleic acid in algae having regulatory and catalytic roles as well as involvement in protein synthesis.
A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.

Role of a novel photosystem II-associated carbonic anhydrase in photosynthetic carbon assimilation in Chlamydomonas reinhardtii. (1/1304)

Intracellular carbonic anhydrases (CA) in aquatic photosynthetic organisms are involved in the CO2-concentrating mechanism (CCM), which helps to overcome CO2 limitation in the environment. In the green alga Chlamydomonas reinhardtii, this CCM is initiated and maintained by the pH gradient created across the chloroplast thylakoid membranes by photosystem (PS) II-mediated electron transport. We show here that photosynthesis is stimulated by a novel, intracellular alpha-CA bound to the chloroplast thylakoids. It is associated with PSII on the lumenal side of the thylakoid membranes. We demonstrate that PSII in association with this lumenal CA operates to provide an ample flux of CO2 for carboxylation.  (+info)

Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that encode novel proteins containing ankyrin repeats and WW domains. (2/1304)

Genes that are expressed only in the young zygote are considered to be of great importance in the development of an isogamous green alga, Chlamydomonas reinhardtii. Clones representing the Zys3 gene were isolated from a cDNA library prepared using zygotes at 10 min after fertilization. Sequencing of Zys3 cDNA clones resulted in the isolation of two related molecular species. One of them encoded a protein that contained two kinds of protein-to-protein interaction motifs known as ankyrin repeats and WW domains. The other clone lacked the ankyrin repeats but was otherwise identical. These mRNA species began to accumulate simultaneously in cells beginning 10 min after fertilization, and reached maximum levels at about 4 h, after which time levels decreased markedly. Genomic DNA gel-blot analysis indicated that Zys3 was a single-copy gene. The Zys3 proteins exhibited parallel expression to the Zys3 mRNAs at first, appearing 2 h after mating, and reached maximum levels at more than 6 h, but persisted to at least 1 d. Immunocytochemical analysis revealed their localization in the endoplasmic reticulum, which suggests a role in the morphological changes of the endoplasmic reticulum or in the synthesis and transport of proteins to the Golgi apparatus or related vesicles.  (+info)

Photosystem I is indispensable for photoautotrophic growth, CO2 fixation, and H2 photoproduction in Chlamydomonas reinhardtii. (3/1304)

Certain Chlamydomonas reinhardtii mutants deficient in photosystem I due to defects in psaA mRNA maturation have been reported to be capable of CO2 fixation, H2 photoevolution, and photoautotrophic growth (Greenbaum, E., Lee, J. W., Tevault, C. V., Blankinship, S. L. , and Mets, L. J. (1995) Nature 376, 438-441 and Lee, J. W., Tevault, C. V., Owens, T. G.; Greenbaum, E. (1996) Science 273, 364-367). We have generated deletions of photosystem I core subunits in both wild type and these mutant strains and have analyzed their abilities to grow photoautotrophically, to fix CO2, and to photoevolve O2 or H2 (using mass spectrometry) as well as their photosystem I content (using immunological and spectroscopic analyses). We find no instance of a strain that can perform photosynthesis in the absence of photosystem I. The F8 strain harbored a small amount of photosystem I, and it could fix CO2 and grow slowly, but it lost these abilities after deletion of either psaA or psaC; these activities could be restored to the F8-psaADelta mutant by reintroduction of psaA. We observed limited O2 photoevolution in mutants lacking photosystem I; use of 18O2 indicated that this O2 evolution is coupled to O2 uptake (i.e. respiration) rather than CO2 fixation or H2 evolution. We conclude that the reported instances of CO2 fixation, H2 photoevolution, and photoautotrophic growth of photosystem I-deficient mutants result from the presence of unrecognized photosystem I.  (+info)

Induction of coproporphyrinogen oxidase in Chlamydomonas chloroplasts occurs via transcriptional regulation of Cpx1 mediated by copper response elements and increased translation from a copper deficiency-specific form of the transcript. (4/1304)

Coproporphyrinogen III oxidase, encoded by a single nuclear gene in Chlamydomonas reinhardtii, produces three distinct transcripts. One of these transcripts is greatly induced in copper-deficient cells by transcriptional activation, whereas the other forms are copper-insensitive. The induced form of the transcript was expressed coordinately with the cytochrome c6-encoding (Cyc6) gene, which is known to be transcriptionally regulated in copper-deficient cells. The sequence GTAC, which forms the core of a copper response element associated with the Cyc6 gene, is also essential for induction of the Cpx1 gene, suggesting that both are targets of the same signal transduction pathway. The constitutive and induced Cpx1 transcripts have the same half-lives in vivo, and all encode the same polypeptide with a chloroplast-targeting transit sequence, but the shortest one representing the induced form is a 2-4-fold better template for translation than are either of the constitutive forms. The enzyme remains localized to a soluble compartment in the chloroplast even in induced cells, and its abundance is not affected when the tetrapyrrole pathway is manipulated either genetically or by gabaculine treatment.  (+info)

Group II intron splicing in Escherichia coli: phenotypes of cis-acting mutations resemble splicing defects observed in organelle RNA processing. (5/1304)

The mitochondrial group IIB intron rI1, from the green algae Scenedesmus obliquus ' LSUrRNA gene, has been introduced into the lacZ gene encoding beta-galacto-sidase. After DNA-mediated transformation of the recombinant lacZ gene into Escherichia coli, we observed correct splicing of the chimeric precursor RNA in vivo. In contrast to autocatalytic in vitro self-splicing, intron processing in vivo is independent of the growth temperature, suggesting that in E.coli, trans -acting factors are involved in group II intron splicing. Such a system would seem suitable as a model for analyzing intron processing in a prokaryotic host. In order to study further the effect of cis -mutations on intron splicing, different rI1 mutants were analyzed (with respect to their splicing activity) in E.coli. Although the phenotypes of these E. coli intron splicing mutants were identical to those which can be observed during organellar splicing of rI1, they are different to those observed in in vitro self-splicing experiments. Therefore, in both organelles and prokaryotes, it is likely that either similar splicing factors or trans -acting factors exhibiting similar functions are involved in splicing. We speculate that ubiquitous trans -acting factors, via recent horizontal transfer, have contributed to the spread of group II introns.  (+info)

Group II intron splicing in chloroplasts: identificationof mutations determining intron stability and fate of exon RNA. (6/1304)

In order to investigate in vivo splicing of group II introns in chloroplasts, we previously have integrated the mitochondrial intron rI1 from the green alga Scenedesmus obliquus into the Chlamydomonas chloroplast tscA gene. This construct allows a functional analysis of conserved intron sequences in vivo, since intron rI1 is correctly spliced in chloroplasts. Using site-directed mutagenesis, deletions of the conserved intron domains V and VI were performed. In another set of experiments, each possible substitution of the strictly conserved first intron nucleotide G1 was generated, as well as each possible single and double mutation of the tertiary base pairing gamma-gamma ' involved in the formation of the intron's tertiary RNA structure. In most cases, the intron mutations showed the same effect on in vivo intron splicing efficiency as they did on the in vitro self-splicing reaction, since catalytic activity is provided by the intron RNA itself. In vivo, all mutations have additional effects on the chimeric tscA -rI1 RNA, most probably due to the role played by trans -acting factors in intron processing. Substitutions of the gamma-gamma ' base pair lead to an accumulation of excised intron RNA, since intron stability is increased. In sharp contrast to autocatalytic splicing, all point mutations result in a complete loss of exon RNA, although the spliced intron accumulates to high levels. Intron degradation and exon ligation only occur in double mutants with restored base pairing between the gamma and gamma' sites. Therefore, we conclude that intron degradation, as well as the ligation of exon-exon molecules, depends on the tertiary intron structure. Furthermore, our data suggest that intron excision proceeds in vivo independent of ligation of exon-exon molecules.  (+info)

Identification of cis-acting RNA leader elements required for chloroplast psbD gene expression in Chlamydomonas. (7/1304)

The psbD mRNA of Chlamydomonas reinhardtii is one of the most abundant chloroplast transcripts and encodes the photosystem II reaction center polypeptide D2. This RNA exists in two forms with 5' untranslated regions of 74 and 47 nucleotides. The shorter form, which is associated with polysomes, is likely to result from processing of the larger RNA. Using site-directed mutagenesis and biolistic transformation, we have identified two major RNA stability determinants within the first 12 nucleotides at the 5' end and near position -30 relative to the AUG initiation codon of psbD. Insertion of a polyguanosine tract at position -60 did not appreciably interfere with translation of psbD mRNA. The same poly(G) insertion in the nac2-26 mutant, which is known to be deficient in psbD mRNA accumulation, stabilized the psbD RNA. However, the shorter psbD RNA did not accumulate, and the other psbD RNAs were not translated. Two other elements were found to affect translation but not RNA stability. The first comprises a highly U-rich sequence (positions -20 to -15), and the second, called PRB1 (positions -14 to -11), is complementary to the 3' end of the 16S rRNA. Changing the PRB1 sequence from GGAG to AAAG had no detectable effect on psbD mRNA translation. However, changing this sequence to CCUC led to a fourfold diminished rate of D2 synthesis and accumulation. When the psbD initiation codon was changed to AUA or AUU, D2 synthesis was no longer detected, and psbD RNA accumulated to wild-type levels. The singular organization of the psbD 5' untranslated region could play an important role in the control of initiation of psbD mRNA translation.  (+info)

Direct measurement of inter-doublet elasticity in flagellar axonemes. (8/1304)

The outer doublet microtubules in ciliary and flagellar axonemes are presumed to be connected with each other by elastic links called the inter-doublet links or the nexin links, but it is not known whether there actually are such elastic links. In this study, to detect the elasticity of the putative inter-doublet links, shear force was applied to Chlamydomonas axonemes with a fine glass needle and the longitudinal elasticity was determined from the deflection of the needle. Wild-type axonemes underwent a high-frequency, nanometer-scale vibration in the presence of ATP. When longitudinal shear force was applied, the average position of the needle tip attached to the axoneme moved linearly with the force applied, yielding an estimate of spring constant of 2.0 (S.D.: 0.8) pN/nm for 1 microm of axoneme. This value did not change in the presence of vanadate, i.e., when dynein does not form strong cross bridges. In contrast, it was at least five times larger when ATP was absent, i.e., when dynein forms strong cross bridges. The measured elasticity did not significantly differ in various mutant axonemes lacking the central-pair microtubules, a subset of inner-arm dynein, outer-arm dynein, or the radial spokes, although it was somewhat smaller in the latter two mutants. It was also observed that the shear displacement in an axoneme in the presence of ATP often took place in a stepwise manner. This suggests that the inter-doublet links can reversibly detach from and reattach to the outer doublets in a cooperative manner. This study thus provides the first direct measure of the elasticity of inter-doublet links and also demonstrates its dynamic nature.  (+info)

Cell growth is tightly coupled to nutrient availability. The target of rapamycin (TOR) kinase transmits nutritional and environmental cues to the cellular growth machinery. TOR functions in two distinct multiprotein complexes, termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). While the structure and functions of TORC1 are highly conserved in all eukaryotes, including algae and plants, TORC2 core proteins seem to be missing in photosynthetic organisms. TORC1 controls cell growth by promoting anabolic processes, including protein synthesis and ribosome biogenesis, and inhibiting catabolic processes such as autophagy. Recent studies identified rapamycin-sensitive TORC1 signaling regulating cell growth, autophagy, lipid metabolism, and central metabolic pathways in the model unicellular green alga Chlamydomonas reinhardtii. The central role that microalgae play in global biomass production, together with the high biotechnological potential of these organisms in biofuel production, has drawn attention
Read Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
RNA pyrophosphohydrolase (RppH) catalyzes the removal of pyrophosphate from 5 triphosphorylated RNAs thereby initiating RNA degradation. The enzyme has originally been identified in bacteria but homologs are present in eukaryotes where they are thought to be located in plastids or mitochondria. A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5rppH-GFP construct.. A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, sequencing, and DNA and RNA blotting techniques were used to indentify positive transformants at the DNA ...
The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. Using a forward genetics approach, we have identified and characterized a mutant x32, which lacks a predicted protein named CGLD1 (Conserved in Green Lineage and Diatom 1) in GreenCut2, under normal and stress conditions. We show that loss of CGLD1 resulted in minimal photoautotrophic growth and PSII activity in the organism. We observed reduced amount of PSII complex and core subunits in the x32 mutant based on blue-native (BN)/PAGE and immunoblot analysis. Moreover, x32 exhibited increased sensitivity to high-light stress and altered tolerance to different reactive oxygenic species (ROS) stress treatments, i.e. decreased resistance to H2O2/or tert-Butyl hydroperoxide (t-BOOH) and increased tolerance to neutral red (NR) and rose bengal (RB) that induce the formation of singlet oxygen, respectively. Further analysis via quantitative real-time PCR (qRT-PCR)
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families.Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes. ...
The data presented here suggest that 3′ end processing may be required for translation of atpB and rbcLmRNAs in Chlamydomonas chloroplasts. Unprocessed atpB transcripts, defined as those that do not accumulate as an abundant size class of approximately 2 kb, were only present in nonpolysomal fractions. Processed mRNAs were present in both polysomal and nonpolysomal fractions. Since the 3′ ends of most chloroplast transcripts are generated from longer pre-mRNAs by exo- and/or endonucleolytic mechanisms (17, 36, 37, 44, 47), this 3′ processing apparatus may interact with or signal the translational machinery.. Our ability to detect a heterogeneous collection of putative processing intermediates or incorrectly processed transcripts for atpBand rbcL suggests that these molecules are relatively stable in the chloroplast. When they were analyzed by RNase protection, it was possible to detect partially processed transcripts in theChlamydomonas chloroplast petD-trnR region (29), and in certain ...
TY - JOUR. T1 - The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii. AU - Ozawa, Shin Ichiro. AU - Cavaiuolo, Marina. AU - Jarrige, Domitille. AU - Kuras, Richard. AU - Rutgers, Mark. AU - Eberhard, Stephan. AU - Drapier, Dominique. AU - Wollman, Francis André. AU - Choquet, Yves. PY - 2020/4/1. Y1 - 2020/4/1. N2 - In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. Here, we report the identification of the octotricopeptide repeat protein MTHI1, which is critical for the biogenesis of chloroplast ATP synthase oligomycin-sensitive chloroplast coupling factor. Unlike most trans-acting factors characterized so far in Chlamydomonas, which control the expression of a single gene, MTHI1 targets two distinct transcripts: it is required for the accumulation and translation of atpH mRNA, encoding a subunit of ...
Ergosterol is the major sterol found in the membranes of Chlamydomonas reinhardtii. While past studies have identified some ergosterol mutants in C. reinhardtii, very little is known about sterol biosynthesis pathways in this species. With the elucidation of the Chlamydomonas genome, bioinformatics analysis has allowed us to determine potential genes involved in ergosterol biosynthesis. With this knowledge, a working model of the pathway was designed for future analysis. Several of the ergosterol biosynthetic genes were analyzed in respect to their role and involvement in flagellar regeneration. These genes were upregulated during the regrowth of the flagella. Also Chlamydomonas strains lacking flagella were analyzed by Q-RT PCR to determine what role ergosterol biosynthetic genes played in the absence of their flagella. Finally, one of the genes with homology to the yeast sterol C-5 desaturase, ERG3, was chosen for further analysis. To test whether ERG3 of C. reinhardtii had a similar function, yeast
The unicellular green alga Chlamydomonas reinhardtii is an ideal model organism for studies of ciliary function and assembly. In assays for biological and biochemical effects of various factors on flagellar structure and function, synchronous culture is advantageous for minimizing variability. Here, we have characterized a method in which 100% synchronization is achieved with respect to flagellar length but not with respect to the cell cycle. The method requires inducing flagellar regeneration by amputation of the entire cell population and limiting regeneration time. This results in a maximally homogeneous distribution of flagellar lengths at 3 h postamputation. We found that time-limiting new protein synthesis during flagellar synchronization limits variability in the unassembled pool of limiting flagellar protein and variability in flagellar length without affecting the range of cell volumes. We also found that long- and short-flagella mutants that regenerate normally require longer and ...
The unicellular green alga Chlamydomonas reinhardtii excels at acclimating to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day to coordinate cellular growth before undergoing cell division. Parallel measurements of select metabolites and pigments, physiological parameters and a subset of proteins allow us to infer metabolic events and to evaluate the impact of the transcriptome on the proteome. Among new findings is the observation that Chlamydomonas exhibits low respiratory activity at night and relies instead on fermentative metabolism; we propose that the ferredoxin FDX9 acts as the electron donor to fermentative hydrogenases. The light stress responsive genes PSBS, LHCSR1 and LHCSR3 all show an acute response to light at dawn under abrupt dark-to light transitions. LHCSR3 genes also ...
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis ...
Chlamydomonas reinhardtii contains a factor that can replace adenosine 3:5-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. The factor cochromatographs and coelectrophoreses with authentic cAMP, and is inactivated by beef heart cyclic nucleotide phosphodiesterase. When C. reinhardtii is exposed to aminophylline (theophylline(2) ethylenediamine), the concentration of the factor in the cells increases within 1 hr, from about 25 pmol of cAMP equivalents per g dry weight to more than 250 pmol. Cyclic nucleotide phosphodiesterase activity is present in crude extract of C. reinhardtii and is inhibited by theophylline. We conclude that cAMP occurs in C. reinhardtii and that the endogenous concentration is governed at least in part by a theophylline-sensitive cyclic nucleotide phosphodiesterase. These findings provide a sound basis for attributing the effects of methylxanthines on flagellar function and regeneration in C. reinhardtii to the resultant elevation of endogenous cAMP
Studies of the biogenesis of the photosynthetic protein complexes in the unicellular green alga Chlamydomonas reinhardtii have pointed to the importance of the concerted expression of nuclear and chloroplast genomes. The accumulation of chloroplast- and nuclear-encoded subunits is concerted, most of …
The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. Current models of the CCM postulate that aseries of ion transporters bring HCO3- from outside the cell to thethylakoid lumen, where the carbonic anhydrase CAH3 dehydratesaccumulated HCO3- to CO2, raising the CO2 concentration forRubisco. Previously, HCO3- transporters have been identified atboth the plasma membrane and the chloroplast envelope, butthe transporter thought to be on the thylakoid membrane hasnot been identified. Three paralogous genes (BST1, BST2, BST3)belonging to the bestrophin family have been found to be upregulatedin low CO2 conditions, and their expression is controlledby CIA5, a transcription factor that controls many CCM genes.YFP fusions demonstrate that all three proteins are located onthe thylakoid membrane, and interactome studies indicate thatthey might associate with chloroplast CCM components. A singlemutant defective in ...
The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added ligand, THB1 coordinates the heme iron with the canonical proximal histidine and a distal lysine. In the cyanomet state, THB1 is structurally similar to other known truncated hemoglobins, particularly the heme domain of Chlamydomonas eugametos LI637, a light-induced chloroplastic hemoglobin. Recombinant THB1 is capable of binding nitric oxide (NO(*)) in either the ferric or ferrous state and has efficient NO(*) dioxygenase activity. By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is
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In this report, we present novel evidence linking FLP to high-affinity Fe transport and propose that FLP is a ferroxidase, functioning in the reoxidation of Fe2+ before its uptake into the cell. Initial evidence in support of this hypothesis comes from the deduced FLP amino acid sequence. FLP contains two multicopper oxidase I and one multicopper oxidase signature II motifs. In addition, the amino acid sequence of FLP shows the highest homology to multicopper oxidases in mammals (HEPH and ceruloplasmin) and yeast (FET3). These proteins are ferroxidases that are themselves involved in high-affinity Fe assimilation (Stearman et al., 1996;Askwith and Kaplan, 1998; Mukhopadhyay et al., 1998; Attieh et al., 1999; Vulpe et al., 1999). The involvement of FLP in Fe homeostasis is evident from the regulation of its synthesis. Both the transcription of the FLP gene and synthesis of FLP are greatly increased in Fe-deficient cells and reversed after resupply of Fe. Although we have not demonstrated the ...
TY - JOUR. T1 - Translational regulation of light-harvesting complex expression during photoacclimation to high-light in Chlamydomonas reinhardtii. AU - McKim, S M. AU - Durnford, D G. PY - 2006/11/14. Y1 - 2006/11/14. N2 - When challenged with excess light, the green alga Chlamydomonas reinhardtii responds, in part, by down-regulating light harvesting capacity at Photosystem II while concomitantly reorganising cellular metabolism to increase sink capacity. We examined the role of translational control during different stages of photoacclimation by analysing polysome profiles of two different light-harvesting complex (LHC) genes encoding a major LHCII component (Lhcbm) and CP29 (Lhcb4) plus iron superoxide dismutase (FeSOD), and through measurement of protein synthesis by in vivo labelling. Within two hours following transfer of low-light (LL) acclimated cultures into high-light (HL), Lhcbm transcripts are off-loaded from polysomes indicating a decline in translational initiation. Lhcbm ...
We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.. ...
Fusion of green fluorescent protein (GFP) to proteins is a powerful method to investigate dynamic processes in vivo. The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP-tagging of proteins was employed in order to analyse proteins of the flagellar basal apparatus. Striated fiber assembling (SFA), centrin and deflagellation induced protein of 13 kDa (DIP13) were tagged with GFP at the C-terminal domain. In addition SFA was tagged at the N-terminal domain. The chimeric genes were stably transformed in C. reinhardtii. SFA is the mayor component of the striated microtubule associated fibers (SMAFS). GFP tagged SFA was incorporated into this fibers. N-terminal tagged SFA had similar properties like the wild-type protein. The length of the fibers increased with the strength of expression. The head domain of SFA is essential for fiber formation and photobleaching experiments did not show a pronounced dynamic of the fibers. The ...
Carbonic anhydrase (CA) is a zinc containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3-. There are three evolutionarily unrelated CA families designated alpha, beta and gamma CA. Vertebrates have members of the alpha CA family, while higher plants, algae and cyanobacteria have members belonging to all three CA families. In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta CAs. This dissertation describes the identification and characterization of new CA genes from C. reinhardtii. Four new CA or CA like genes have been discovered including two beta CAs and two gamma CAs. Three CAs were investigated further including the alpha CA Cah3, one of the new beta CAs, Cah6; and a new gamma CA designated Gclp1 for gamma CA like protein. Cah3 is an alpha CA located in the thylakoid. Past studies with two Cah3 mutants, ca-1 and cia3 have shown that Cah3 plays an important role in the CO2 concentrating ...
en] Photosynthetic activities were analyzed in Chlamydomonas reinhardtii mitochondrial mutants affected in different complexes (I, III, IV, I + III, and I + IV) of the respiratory chain. Oxygen evolution curves showed a positive relationship between the apparent yield of photosynthetic linear electron transport and the number of active proton-pumping sites in mitochondria. Although no significant alterations of the quantitative relationships between major photosynthetic complexes were found in the mutants, 77 K fluorescence spectra showed a preferential excitation of photosystem I (PSI) compared with wild type, which was indicative of a shift toward state 2. This effect was correlated with high levels of phosphorylation of light-harvesting complex II polypeptides, indicating the preferential association of light-harvesting complex II with PSI. The transition to state 1 occurred in untreated wild-type cells exposed to PSI light or in 3-(3,4-dichlorophenyl)-1,1-dimethylurea-treated cells exposed ...
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells ...
To make the CrPKD2-GFP fusion, the C. reinhardtii bacterial artificial chromosome clone 18K16 (https://www.genome.clemson.edu/cgi-bin/orders) was cut with EcoRV and SpeI, generating an ∼18-kb fragment, which included the entire CrPKD2 gene and its promoter. This fragment was subcloned into the EcoRV and SpeI sites of pBluescript II KS+ (Stratagene), generating a plasmid named pHK25. This plasmid was cut with HindIII, generating three fragments. One of these fragments, an 8-kb fragment containing the promoter and most of the genomic DNA encoding CrPKD2, was cloned into the HindIII site of the pBluescript KS+, generating pHK28. A second fragment, a 5.8-kb fragment containing the last two introns, exons, the 3′ UTR of CrPKD2, and the KS+ vector, was religated to produce pHK26.. To tag the CrPKD2 gene, we cloned the GFP gene into a unique EcoRI site in intron 11, flanked by the first intron of RBCS2 (Goldschmidt-Clermont and Rahire, 1986). For this, the two ends of the intron were subcloned as ...
MicroRNAs play an important role in abiotic stress responses in higher plants and animals, but their role in stress adaptation in algae remains unknown. In this study, the expression of identified and putative miRNAs in Chlamydomonas reinhardtii was assessed using quantitative polymerase chain reaction; some of the miRNAs (Cre-miR906-3p) were up-regulated, whereas others (Cre-miR910) were downregulated when the species was subjected to multiple abiotic stresses. With degradome sequencing data, we also identified ATP4 (the d-subunit of ATP synthase) and NCR2 (NADPH: cytochrome P450 reductase) as one of the several targets of Cre-miR906-3p and Cre-miR910, respectively. Q-PCR data indicated that ATP4, which was expressed inversely in relation to Cre-miR906-3p under stress conditions. Overexpressing of Cre-miR906-3p enhanced resistance to multiple stresses; conversely, overexpressing of ATP4 produced the opposite effect. These data of Q-PCR, degradome sequencing and adaptation of overexpressing ...
The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the nitrate reductase structural gene nit-1, have been related to nitrate transport. Mutant strains defective in nitrate transport and having an active nitrate reductase have bee …
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S.P. Mayfield, P. Lee, P. Pettersson, J. Marìn-Navarro, A. Manuell, M. Muto, M. Tran We have developed a system for expressing recombinant proteins, including human therapeutic proteins, in the chloroplast of the eukaryotic green alga Chlamydomonas reinhardtii. Expression of therapeutic proteins in eukaryotic algae offers several advantages over more traditional protein expression systems. Algae are efficient at producing complex mammalian proteins, stable transgenic lines can be generated in a few weeks, and algal systems can be scaled to high levels for a fraction of the cost of traditional fermentation systems.. We have expressed several recombinant proteins in algae, including human monoclonal antibodies. Antibodies are complex multiprotein molecules that are difficult to express in simple expression systems and expensive to produce in mammalian cell culture. We have had good success in producing these complex proteins in algal chloroplasts. We have also expressed eukaryotic protein toxins, ...
Our initial mapping data placed Mcd4 into two possible locations. In both cases, it was desirable to generate bacterial artificial chromosome (BAC) contigs for eventual complementation, as well as additional markers. As a case study, we describe how BAC contigs can be extended using Chlamydomonas resources and our experience in generating site-specific markers.. Several BAC libraries have been constructed for Chlamydomonas, two of which are available through the Clemson Genomics Institute (http://www.genome.clemson.edu/groups/bac/). In addition, BAC contigs (http://www.biology.duke.edu/chlamy_genome/BAC/index.html) have been assembled for most of the STS and RFLP markers. In the case of mcd4, Gsp1 resides on scaffold 2 and CNA45 on scaffold 66. A 41-BAC contig exists for scaffold 2 covering approximately 1,000 kb (R. Nguyen, personal communication), and a smaller contig is linked to scaffold 66 and CNA45 (Fig. 6). An unknown amount of DNA separates scaffolds 2 and 66; such discontinuities in the ...
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The project covers the whole process chain from optimized biomass production to product development and exploitation. In a first step, two industrial bioproduction platforms will be explored: the green alga Botryococcus braunii and the green microalga Chlamydomonas reinhardtii, to which the unique hydrocarbon and polysaccharides producing genes from Botryococcus will be transferred. Biomass cultivation is targeted to reach a pilot scale.
Centrin, a 20-kD phosphoprotein with four calcium-binding EF-hands, is present in the centrosome/basal body apparatus of the green alga Chlamydomonas reinhardtii in three distinct locations: the nucleus-basal body connectors, the distal striated fibers, and the flagellar transition regions. In each location, centrin is found in fibrous structures that display calcium-mediated contraction. The mutant vfl2 has structural defects at all of these locations and is defective for basal body localization and/or segregation. We show that the vfl2 mutation is a G-to-A transition in the centrin structural gene which converts a glutamic acid to a lysine at position 101, the first amino acid of the E-helix of the proteins third EF-hand. This proves that centrin is required to construct the nucleus-basal body connectors, the distal striated fibers, and the flagellar transition regions, and it demonstrates the importance of amino acid 101 to normal centrin function. Based on immunofluorescence analysis using ...
While conducting a screen for genes affecting pyrenoid function in the green alga Chlamydomonas reinhardtii, graduate student and the studys first author Alan Itakura and postdoctoral researcher Leif Pallesen, both in the Jonikas group, uncovered a gene called SAGA1 (Starch Granules Abnormal-1), the loss of which causes cells to grow poorly. When the researchers, including the studys co-first author, Kher Xing (Cindy) Chan in Howard Griffiths group at the University of Cambridge, examined the mutant cells, they noticed that SAGA1 mutants possess multiple pyrenoids-up to 10 per cell. This was surprising since normal cells almost always contain just one pyrenoid. Intrigued, the team decided to investigate further.. Because the SAGA1 protein is predicted to contain a starch-binding domain, the researchers first explored whether loss of the SAGA1 gene affects the architecture of the starch plates that make up the pyrenoid sheath. Indeed, the pyrenoids in SAGA1-deficient cells have fewer and ...
High light (HL) stress adversely affects growth, productivity and viability of photosynthetic organisms. The green alga Chlamydomonas reinhardtii is a model system to study photosynthesis and light stress. Comparative proteomics of wild-type and two very high light (VHL)-resistant mutants, VHLR-S4 and VHLR-S9, revealed complex alterations in response to excess light. A twodimensional reference map of the soluble subproteome was constructed representing about 1500 proteins. A total of 83 proteins from various metabolic pathways were identified by peptide mass fingerprinting. Quantitative comparisons of 444 proteins showed 105 significantly changed proteins between wild type and mutants under different light conditions. Commonly, more proteins were decreased than increased, but different proteins were affected in each genotype. Proteins uniquely altered in either VHLR mutant may be involved in VHL resistance. Such candidate proteins similarly altered without light stress, thus possibly ...
The goal of our scientific work is the molecular analysis of the biogenesis of photosynthetic thylakoid membranes which represent one of the most complex energy-transducing membranes currently known. We especially ask the questions how the biogenesis process is organized in space, what the molecular working mode of assisting factors is and how these factors developed by evolutionary means.. A second aspect of our work concerns the transformation of a cyanobacterium into an organelle of the plant cell, namely the chloroplast. This domestification of a former free-living procaryote throughout evolution was enabled by the advention of an intracellular communication system harmonizing gene expression in the former cyanobacterium and its host. This system is based on regulatory RNA/protein compexes which we study by applying genetic and biochemical techniques in the unicellular model green alga Chlamydomonas reinhardtii.. ...
Editors note: I first read about this in The Celestine Prophecy (awesome book, btw) back in 1997. A biological research team at Bielefeld University has made a groundbreaking discovery showing that plants can draw an alternative source of energy from other plants. This finding could also have a major impact on the future of bioenergy eventually providing the evidence to show that people draw energy from others in much the same way.. Members of Professor Dr. Olaf Kruses biological research team have confirmed for the first time that a plant, the green alga Chlamydomonas reinhardtii, not only engages in photosynthesis, but also has an alternative source of energy: it can draw it from other plants. The research findings were released this week in the online journal Nature Communications published by the renowned journal Nature.. Flowers need water and light to grow and people are no different. Our physical bodies are like sponges, soaking up the environment. This is exactly why there are certain ...
Nitrite plays an important role in the nitrogen metabolism of most cells, including Chlamydomonas reinhardtii. We have shown that vegetative cells of C. reinhardtii are attracted by nitrite. The Nia1nit2 mutant with defects in genes encoding the nitrate reductase and regulatory protein NIT2 respectively was found to exhibit normal chemotaxis to nitrite. The data suggest that chemotaxis events appear to be specific and independent of those involved in nitrate assimilation. Unlike vegetative cells and noncompetent pregametes, mature gametes did not show chemotaxis to nitrite. Just like gamete formation, the change in chemotaxis mode is controlled by the sequential action of two environmental cues, removal of nitrogen from the medium and light. Comparative analysis of wild-type and RNAi strains with reduced level of phototropin has indicated that switch-off of chemotaxis towards nitrite is dependent on phototropin. The studies revealed individual elements of the phototropin-dependent signal transduction
The 10th International Conference on the Cell and Molecular Biology of Chlamydomonas will be held in Vancouver, Canada, June 11-16, 2002. Please visit http://www.quarmby.ca/chlamy2002 to register for the meeting and reserve your accommodation. Its going to be a great meeting and we look forward to seeing you all there. A preliminary program outline is now on the program page to facilitate your travel plans. -- Elizabeth Harris chlamy at duke.edu Chlamydomonas Genetics Center home page: http://www.biology.duke.edu/chlamy/ Resource Center for Chlamy genome project: http://www.biology.duke.edu/chlamy_genome/crc.html ------- End of forwarded message ...
Small rab-related GTPase; Small GTPase-like component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT25. Has very low GTPase activity either because it lacks the conserved catalytic Gln in position 79 or because it requires some GTPase-activating protein (GAP) for GTP turnover (204 aa ...
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1FCT: NMR structures of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution.
in Molecular and General Genetics (1998), 259(3), 294-8. In Chlamydomonas reinhardtii, mutants defective in the cytochrome pathway of respiration lack the capacity to grow under heterotrophic conditions (in darkness on acetate). In the dark- strain duM18, a + 1 ... [more ▼]. In Chlamydomonas reinhardtii, mutants defective in the cytochrome pathway of respiration lack the capacity to grow under heterotrophic conditions (in darkness on acetate). In the dark- strain duM18, a + 1 T addition in a run of four Ts, located at codon 145 of the mitochondrial cox1 gene encoding subunit I of cytochrome c oxidase, is responsible for the mutant phenotype. A leaky revertant (su11) that grows heterotrophically at a lower rate than wild-type cells was isolated from dum18. Its respiration sensitivity to cyanide was low and its cytochrome c oxidase activity was only 4% of that of the wild-type enzyme. Meiotic progeny obtained from crosses between revertant and wild-type cells inherited the phenotype of the mt- ...
This model does not account for two published observations: An mt+ strain carrying the MID gene transposed to an autosome differentiates as minus, as do mt+ cells transformed with the MID gene, even though neither possesses a copy of the MTD1 gene (Ferris and Goodenough 1997). To reconcile these observations with the results reported here, we are led to propose that plus gametes express a system, the MTD1-equivalent system, that is functionally equivalent to the MTD1 system but achieves this outcome without requiring the Mtd1 protein itself. When MID is introduced into a plus background, the MTD1-equivalent system enables sufficiently high MID expression to allow transformants to undergo minus differentiation, albeit success is usually incomplete (see results and Ferris and Goodenough 1997), meaning that the MTD1-equivalent system is not repressible by Mid. Importantly, at least one essential gene in the posited plus MTD-equivalent system must be resident in the MT+ locus. If the system were ...
Media, culture conditions, and strains: M medium is Sager and Granick medium I with Hutners trace elements (Harris 1989). R medium is M medium with 0.1% sodium acetate added. In RNA and DNA medium the NH4Cl concentration is reduced 10-fold and 200 μg/ml of arginine is added. Solid media contain 1.5% agar (R, RNA), 2% agar (D, DNA), or 4% agar (Z).. Both liquid cultures and plates were incubated at 25° in continuous white fluorescent light. All of the mutant strains described in this article were isolated in strain 137c mt+ except strain 6-9, which was isolated in mt-. Strains bearing intragenic complementing arg7 alleles were used to recover diploids for complementation analyses of eyespot-assembly mutations (Matagne 1978; Harris 1989).. Mutagenesis, enrichment, and screening for phototaxis-negative mutants: Mutagenesis: Strain 137c was grown on R plates for two days. The cells were scraped from the surface and resuspended in liquid M medium. Between 2 and 7 × 107 cells in 1 ml were pipetted ...
NADPH-dependent flavin reductase; Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe-S cluster of the anamorsin/DRE2 homolog (620 aa ...
The time course of and the influence of light intensity and light quality on the induction of a mitochondrial carbonic anhydrase (CA) in the unicellular green alga Chlamydomonas reinhardtii was characterized using western and northern blots. This CA was expressed only under low-CO2 conditions (ambient air). In asynchronously grown cells, the mRNA was detected 15 min after transfer from air containing 5% CO2 to ambient air, and the 21-kD polypeptide was detected on western blots after 1 h. When transferred back to air containing 5% CO2, the mRNA disappeared within 1 h and the polypeptide was degraded within 3 d. Photosynthesis was required for the induction in asynchronous cultures. The induction increased with light up to 500 mu mol m(-2) s(-1), where saturation occurred. In cells grown synchronously, however, expression of the mitochondrial CA was also detected in darkness. Under such conditions the expression followed a circadian rhythm, with mRNA appearing in the dark 30 min before the light ...
TY - JOUR. T1 - Enzymatic properties of the ferredoxin-dependent nitrite reductase from chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production. AU - Hirasawa, Masakazu. AU - Tripathy, Jatindra N.. AU - Sommer, Frederik. AU - Somasundaram, Ramasamy. AU - Chung, Jung Sung. AU - Nestander, Matthew. AU - Kruthiventi, Mahima. AU - Zabet-Moghaddam, Masoud. AU - Johnson, Michael K.. AU - Merchant, Sabeeha S.. AU - Allen, James Paul. AU - Knaff, David B.. PY - 2010/1. Y1 - 2010/1. N2 - The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are quite similar to those of the ferredoxin-dependent spinach chloroplast nitrite reductase. Computer modeling, based on the published structure of spinach nitrite reductase, predicts ...
When Venus was used as a GOI with this system, a positive correlation was found between paromomycin resistance and Venus fluorescence, indicating that expression from the two ORFs is coupled. It initially seemed possible that a mechanism such as stop-codon read through (Jackson et al. 2012) might result in expression of the GOI and APHVIII products as a single fusion protein that provides paromomycin resistance. However, this appears not to be the case, because the method works equally well when two or three different stop codons are inserted between the ORFs, and/or the ORFs are placed out-of-frame, and Western blotting detected no such fusion products. Thus, it seems most likely that APHVIII is translated by post-termination reinitiation (Kozak 2007; Skabkin et al. 2013), in which the ribosome remains associated with the mRNA after termination, continues scanning, and reinitiates translation at a downstream (or, occasionally, upstream: Skabkin et al. 2013) AUG. Such events have been well ...
A Chlamydomonas reinhardtii chloroplast expression vector, pACTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructe
Like a strict parent, the mother centriole keeps order in the cell by telling other organelles where to sit, according to new work by Jessica Feldman, Wallace Marshall (University of California, San Francisco, CA), and Stefan Geimer (Universität Bayreuth, Bayreuth, Germany).. Marshalls team is interested in what controls the intracellular geometry of organelle positioning. To address this topic, they focus on one organelle that is well-known for its specific positioning: the centriole.. The tethered pair of mother and daughter centrioles is the major component of the centrosome complex and also promotes the assembly of cilia. Thus cilia can act as a cell surface indicator of centriole positioning. The team used the unicellular alga Chlamydomonas reinhardtii, which normally has two cilia at its apex, to scan for mutants in which cilia were misplaced.. In certain misplaced cilia mutants, the fibers that normally tether mother and daughter centrioles were absent. The team found that whereas the ...
TY - JOUR. T1 - Phospholipid:diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation while synthesizing triacylglycerol in the unicellular green microalga Chlamydomonas reinhardtii. AU - Yoon, K. AU - Han, D. AU - Li, Y. AU - Sommerfeld, M.. AU - Hu, Q.. AU - Sommerfeld, Milton R. PY - 2012. Y1 - 2012. M3 - Article. SP - 3708. EP - 3724. JO - The Plant Cell. JF - The Plant Cell. ER - ...
Cilia and flagella are cell surface organelles with microtubule-based axonemal cores. Although these organelles have been known to biologists for centuries, only in the last five years has it been recognized that cilia are crucial for mammalian embryonic development as well as for the function of multiple adult organs (Pan et al., 2005). Many potential ciliary proteins have been identified in various species in recent years using biochemical, comparative genomic and proteomic methods. Nevertheless, the spectrum of factors required for the formation and/or function of cilia, as well as the molecular mechanisms underlying the regulation of cilia biogenesis, have yet to be fully revealed.. Two multiprotein complexes, the intraflagellar transport (IFT, complex A and B) complexes, are present in the green alga Chlamydomonas reinhardtii (Rosenbaum and Witman, 2002). The IFT complexes move within the flagella, suggesting that they are likely to be involved in the transportation of molecules inside the ...
Photosynthetic microalgae hold promise as green cell factories for sustainable light-driven bio-production processes. These organisms can be cultivated with freely available sunlight energy and CO2 as a sole carbon source, making them ideal chassis for sustainable production processes. Microalgae are already natural sources of many interesting bio-products including carotenoids, lipids, and polysaccharides. However, expanding the range as well as value of the compounds produced by microalgae through genetic engineering can increase the economic competitiveness of light-driven algal production platforms. In comparison to bacteria or yeasts, genetic engineering of eukaryotic microalgae has lagged significantly behind due to characteristically low transgene expression levels. Work in our research group has focused on engineering increased and reliable levels of nuclear transgene expression in the fast growing, Chlorophyceaen microalga Chlamydomonas reinhardtii, with the aim of generating ...
Fingerprint Dive into the research topics of Role of timer and sizer in regulation of Chlamydomonas cell cycle. Together they form a unique fingerprint. ...
Amoroso, G., D. Sueltemeyer, C. Thyssen and H.P. Fock (1998). Uptake of HCO3- and CO2 in cells and chloroplasts from the microalgae Chlamydomonas reinhardtii and Dunaliella tertiolecta. Plant Physiol. 116, 193-201. Asleson, C.M. and P.A. Lefebvre (1998). Genetic analysis of flagellar length control in Chlamydomonas reinhardtii: A new long-flagella locus and extragenic suppressor mutations. Genetics 148, 693-702. Bhattacharya, D. and L. Medlin (1998). Algal phylogeny and the origin of land plants. Plant Physiol. 116, 9-15. Boschetti, A. and K. Schmid (1998). Energy supply for ATP-synthase deficient chloroplasts of Chlamydomonas reinhardii. Plant Cell Physiol. 39, 160-168. Brosch-Salomon, S., M. Hoeftberger, A. Holzinger and U. Luetz-Meindl (1998). Ultrastructural localization of polysaccharides and N-acetyl-D-galactosamine in the secretory pathway of green algae (Desmidiaceae). J. Exp. Bot. 49, 145-153. Calenberg, M., U. Brohsonn, M. Zedlacher and G. Kreimer (1998). Light- and Ca2+-modulated ...
Read UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
In 1933, Marjory Stephenson and her student Stickland reported that cell suspensions catalysed the reduction of methylene blue with H2. Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce hydrogen.[17] In the late 1990s Anastasios Melis discovered that deprivation of sulfur induces the alga to switch from the production of oxygen (normal photosynthesis) to the production of hydrogen. He found that the enzyme responsible for this reaction is hydrogenase, but that the hydrogenase lost this function in the presence of oxygen. Melis also discovered that depleting the amount of sulfur available to the algae interrupted their internal oxygen flow, allowing the hydrogenase an environment in which it can react, causing the algae to produce hydrogen.[18] Chlamydomonas moewusii is also a promising strain for the production of hydrogen.[19][20] ...
A main question for the study of collective motion in living organisms is the origin of orientational polar order, i.e., how organisms align and what are the benefits of such collective behaviour. In the case of micro-organisms swimming at a low Reynolds number, steric repulsion and long-range hydrodynamic interactions are not sufficient to explain a homogeneous polar order state in which the direction of motion is aligned. An external symmetry-breaking guiding field such as a mechanism of taxis appears necessary to understand this phonemonon. We have investigated the onset of polar order in the velocity field induced by phototaxis in a suspension of a motile micro-organism, the algae Chlamydomonas reinhardtii, for density values above the limit provided by the hydrodynamic approximation of a force dipole model. We show that polar order originates from a combination of both the external guiding field intensity and the population density. In particular, we show evidence for a linear dependence of a
The focus of our groups activities is the analysis and manipulation of metabolic pathways. With an increased understanding of the regulation of metabolism, scientists and engineers can rationally manipulate pathways to produce novel compounds or increase the production of specialty compounds. Quantifying metabolic flux is a critical technology that forms the basis for rational metabolic engineering. Our group has been developing the mathematical modeling and experimental tools for the particularly difficult problem of quantifying fluxes in photoautotrophic organisms. The current focus is on quantifying intracellular metabolite fluxes in cyanobacteria, algae and plants by liquid chromatography-mass spectrometry (LC-MS/MS) and gas chromatography-MS (GC/MS). We have also constructed a genome scale metabolic model of the algae Chlamydomonas reinhardtii and performed flux balance analysis on the network, which we are comparing against experimentally determined metabolic fluxes derived from transient ...
Nuclear transformation occurs when the atoms of one element change to become atoms of another element. This is most commonly seen with...
A select set of microalgae are reported to be able to catalyse photobiological H(2) production from water. Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H(2)-producing microalgae. By purging algal cultures with N(2) in the dark and subsequent illumination, it is possible to rapidly induce photobiological H(2) evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H(2). Using this system, five algal isolates capable of H(2) production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H(2) production. H(2)-producing algal species were seen to be ...
Anti-Lhcb4 (CP29) Chlamydomonas reinhardti, Lhcb4, CP29, Lhcbm4 antibody , Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas, Q93WD2, AS06 117
Definition of chlamydomonas - a common single-celled green alga which typically has two flagella for swimming, living in water and moist soil.
TY - CHAP. T1 - Appropriate observables for investigating narrow resonances in Kaon photoproduction off a proton. AU - Mart, Terry. PY - 2011/11/1. Y1 - 2011/11/1. N2 - The existence of non-strange partner of pentaquark, the Jp = 1/2+ narrow resonance, has been investigated by utilizing kaon photoproduction off a proton. It is found that the corresponding mass is 1650 MeV and the appropriate observables for investigating the existence of this resonance are the recoiled hyperon polarization, the beam-recoil double polarization Cx, and differential cross section at backward angles. Future kaon photoproduction experiments should focus on these observables.. AB - The existence of non-strange partner of pentaquark, the Jp = 1/2+ narrow resonance, has been investigated by utilizing kaon photoproduction off a proton. It is found that the corresponding mass is 1650 MeV and the appropriate observables for investigating the existence of this resonance are the recoiled hyperon polarization, the beam-recoil ...
Engineered tissues are highly limited by poor vascularization in vivo, leading to hypoxia. In order to overcome this challenge, we propose the use of photosynthetic biomaterials to provide oxygen. Since photosynthesis is the original source of oxygen for living organisms, we suggest that this could be a novel approach to provide a constant source of oxygen supply independently of blood perfusion. In this study we demonstrate that bioartificial scaffolds can be loaded with a solution containing the photosynthetic microalgae Chlamydomonas reinhardtii, showing high biocompatibility and photosynthetic activity in vitro. Furthermore, when photosynthetic biomaterials were engrafted in a mouse full skin defect, we observed that the presence of the microalgae did not trigger a native immune response in the host. Moreover, the analyses showed that the algae survived for at least 5days in vivo, generating chimeric tissues comprised of algae and murine cells. The results of this study represent a crucial ...
ABOUT CRAG:. CRAG is an independent research institution engaged in leading-edge basic and applied plant and farm animal sciences. CRAG is established as a Consortium of the Spanish National Research Council (CSIC), Institute of Agrifood Research and Technology (IRTA), Autonomous University of Barcelona (UAB), and University of Barcelona (UB). The Center is located at the UAB Campus, and currently hosts 200 members from across the world.. Research Programs at CRAG (from basic science to applied research using plant experimental model systems, crops and farm animals) make extensive use of genomic technologies and large sets of genetic and genomic data (https://biennialreport2016-2017.cragenomica.es/).. RESPONSIBILITIES:. The hired technician will assist in undergoing research aimed at characterizing the components of light, high light and retrograde signaling in the model organisms Arabidopsis thaliana and Chlamydomonas reinhardtii.. The tasks are included within the framework of the project ...
In the GAPDH-CP12-PRK complex, T. elongatus PRK is dimeric, as in solution (SI Appendix, Fig. S5E), and like other plant-type PRKs (6, 7). PRK has an alpha-beta-alpha sandwich fold where the central 9-strand beta-sheet is continuous across the dimer interface (Fig. 3C). The active site cleft lies between 3 loops (residues 137-164, 43-63, and 87-98). The N-terminal helical bundle of CP12 plugs this cleft and sterically blocks the active site (Fig. 3D). The charged patch created by the CP12 motif binds to complementary positively charged regions in the PRK active site (Fig. 3E), which have also been proposed to be important for negatively charged sugar phosphate substrate binding (6). Variants in the CP12 of Chlamydomonas reinhardtii, equivalent to Trp33, Glu37, and Glu38 in the conserved CP12 motif resulted in loss of complex formation (24). The conserved CP12-Trp33 is on the surface and packs against PRK, contradicting a prediction that it is buried (29). CP12-Glu33 makes a salt bridge with ...
May, P., Wienkoop, S., Kempa, S., Usadel, B., Christian, N., Rupprecht, J., Weiss, J., Recuenco-Munoz, L., Ebenhöh, O., Weckwerth, W., Walther, D (2008) Metabolomics- and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii. Genetics. 179:157-66.. Howell, K.A., Narsai, R., Carroll, A., Ivanova, A., Lohse, M., Usadel, B., Millar, A.H., Whelan, J. (200X) Mapping metabolic and transcript temporal switches during germination in Oryza sativa highlights specific transcription factors and the role of RNA instability in the germination process. Plant Physiol. accepted. Crowhurst, R.N., et al. (2008) Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening. BMC Genomics. 9:351.. ...
Algae Detail UTEX Number: 969Class: ChlorophyceaeStrain: Chlamydomonas applanataMedia: Modified Bold 3N MediumOrigin: Williamson Co., Texas, USADescription of L
Algae Detail UTEX Number: 943Class: ChlorophyceaeStrain: Chlamydomonas giganteaMedia: Modified Bold 3N MediumOrigin: Description of Location: Type Culture: NoCo
Fingerprint Dive into the research topics of Nucleus-basal body connector in chlamydomonas: Evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Together they form a unique fingerprint. ...
Introduction Bio 2010 01/31/2014 The objectives of this lab were to understand natural selection and understand the biotic and abiotic characteristics of a niche. In order to understand natural selection in this lab we observed characteristics of three different algae families; Volvox, Chlamydomonas, and Gonium. By observing these algae will give us a better understanding of how a simple cellular organism evolved due to natural selection and are able to survive today. These organisms will give us context into natural selection occurring on bigger organisms as we move on in lab. Similarly, in order to understand biotic and abiotic characteristics of a niche, we observed a transect. By observing a transect will provide insight into how the biosphere works by focussing on biotic and biotic components of a home to many organisms. Procedure 1: The Volvicine Line In this experiment we observed an isogamous, single celled, motile alga called chlamydomonas. We prepared a slide of a living Chlamydomonas ...
Figure 5A. Wildtype and roc15 mutant Chlamydomonas was entrained to a 12:12 light:dark cycle. The cultures were transferred to constant darkness and their tufA expression was measured with luminescence. They were then expressed to 5 minute long pulses of light. The wild type Chlamydomonas responded to the light pulses, evident by a phase shift in tufA expression. The roc15 mutant Chlamydomonas did not exhibit this phase shift. This indicate that roc15 is essential for circadian regulated phase shifts and entrainment. ...
Immunofluorescence micrograph of a Chlamydomonas cell stained with acetylated tubulin antibody to label flagella (green) and with an antibody against the protein EB1 that labels the flagella tip and base (red/yellow). Image from Lotte Pedersen, University of Copenhagen. To previous page ...
The Cytoskeleton Collection includes 250 mAbs recognizing the supportive structural filaments and associated proteins that facilitate cell shape and motility
Alphabetic Listing of Presenting Authors - R. If you have any questions or comments then please send an email to [email protected] ...
pep:novel chromosome:VEGA66:10:33905485:33915883:1 gene:OTTMUSG00000029742 transcript:OTTMUST00000073807 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Rsph4a description:radial spoke head 4 homolog A (Chlamydomonas ...
All algae need access to light and live in oxygenated water. There are more than 7,000 species of green algae, which live in a variety of...
Mohon maaf, kami belum menyediakan peta untuk retret 21-22 Juli ini. Peta yang Bapak lihat adalah peta tempat retret yang sebelumnya ...
Project Noah is a tool that nature lovers can use to explore and document local wildlife and a common technology platform that research groups can use to harness the power of citizen scientists everywhere.
ISBN 978-0-521-64497-6. Tibiletti, T., Auroy, P., Peltier, G. and Caffarri, S. (2016). Chlamydomonas reinhardtii PsbS protein ... Chlamydomonas reinhardtii. Their data concluded that the PsBs protein belongs to a multigene family termed LhcSR proteins, ...
2008). "Toxicity of silver nanoparticles to chlamydomonas reinhardtii". Environ Sci Technol. 42 (23): 8959-64. Bibcode:2008EnST ... Chlamydomanas reinhardtii, but after 2 hours of incubation it was revealed that the algae containing silver nanoparticles were ...
"An energy balance from absorbed photons to new biomass for Chlamydomonas reinhardtii and Chlamydomonas acidophila under neutral ... In Chlamydomonas reinhardtii Photosystem II produces in direct conversion of sunlight 80% of the electrons that end up in the ... Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce ... "Identification of Global Ferredoxin Interaction Networks in Chlamydomonas reinhardtii". Journal of Biological Chemistry. 288 ( ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
"Integration of chloroplast nucleic acid metabolism into the phosphate deprivation response in Chlamydomonas reinhardtii". The ...
Chloroplast-encoded chlB is required for light-independent protochlorophyllide reductase activity in Chlamydomonas reinhardtii ... Later, by the sequence similarity, a similar protein was found in Chlamydomonas algae,[9] showing that this regulatory ... In a different manner, the Chlamydomonas regulatory protein is more complex: It is larger, crosses the thylakoid membrane twice ... The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas. Genes & ...
Transmission electron micrograph of Chlamydomonas reinhardtii, a green alga that contains a pyrenoid surrounded by starch. ... Required for Normal Pyrenoid Formation in Chlamydomonas reinhardtii". Plant Physiology. 156 (2): 884-96. doi:10.1104/pp. ... "Chlamydomonas Ehrenberg, 1833: 288". algaeBASE. Retrieved 19 May 2013.. *^ "Spirogyra Link, 1820: 5". algaeBASE. Retrieved 19 ... J D, Rochaix (1998). The molecular biology of chloroplasts and mitochondria in Chlamydomonas. Dordrecht [u.a.]: Kluwer Acad. ...
Lessons from Chlamydomonas reinhardtii". Plant Physiol. 127 (4): 1500-1507. PMC 1540183. PMID 11743094. doi:10.1104/pp.010807. ...
Grossman AR, Lohr M, Im CS (2004). "Chlamydomonas reinhardtii in the landscape of pigments". Annu. Rev. Genet. 38 (1): 119-73. ...
Grossman AR, Lohr M, Im CS (2004). „Chlamydomonas reinhardtii in the landscape of pigments". Annu. Rev. Genet. 38 (1): 119-73. ...
... and Biochemical Characterization in Wild-Type and Starchless Chlamydomonas reinhardtii". Eukaryotic Cell. 8 (12): 1856-1868. ... "RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii". ...
"Higher plant-like subunit composition of mitochondrial complex I from Chlamydomonas reinhardtii: 31 conserved components among ...
Chlamydomonas reinhardtii in the landscape of pigments. - Annu. Rev. Genet., 2004, kd 38, nr 1, lk 119-173. Resümee. Resümee. ...
Grossman AR, Lohr M, Im CS (2004). "Chlamydomonas reinhardtii in the landscape of pigments". Annu. Rev. Genet. 38: 119-73. doi: ...
Species in the Dunaliella genus are morphogically similar to Chlamydomonas reinhardtii with the main exception being that ...
Recently, cellulases have also been found in green microalgae (Chlamydomonas reinhardtii, Gonium pectorale and Volvox carteri) ...
... reinhardtii Transcription Factor Database. *"Chlamydomonas", a song by Andy Offutt Irwin about the life cycle of ... Chlamydomonas reinhardtii[9]. Ecology[edit]. Widely distributed in freshwater or damp soil.[1]It is generally found in habitat ... The dynamic behaviour of mitochrandia in living zygotes during maturation and meiosis in Chlamydomonas reinhardtii. European ... Chlamydomonas is a genus of green algae consisting of about 325 species[1] all unicellular flagellates, found in stagnant water ...
I think the sulfur starve method of hydrogen production was discovered with Chlamydomonas reinhardtii, a green algae rather ...
Lessons from Chlamydomonas reinhardtii". Plant Physiol. 127 (4): 1500-1507. doi:10.1104/pp. 010807. PMC 1540183. PubMed. ...
萊茵衣藻(英語:Chlamydomonas reinhardtii). *四膜蟲. *釀酒酵母 ...
Work has been done with duckweed Lemna minor,[61] the algae Chlamydomonas reinhardtii[62] and the moss Physcomitrella patens.[ ...
Chlamydomonas reinhardtii on üherakuline rohevetikas, mille põhjal uuritakse fotosünteesi, vibureid ja liikuvust, ainevahetuse ...
The single celled green alga Chlamydomonas reinhardtii, while not an embryophyte itself, contains a green-pigmented chloroplast ... The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas. Dordrecht, Germany: Kluwer Academic. ISBN 978-0-7923- ...
Some such organisms, including the alga Chlamydomonas reinhardtii and cyanobacteria, have evolved a second step in the dark ...
Chlamydomonas reinhardtii)作为模式生物,给细胞生物学提供了重要的
Beberapa organisme meliputi ganggang Chlamydomonas reinhardtii dan cyanobacteria memiliki tahap kedua, yaitu reaksi gelap, yang ...
Herron et al [50] performed laboratory evolution experiments on the single-celled green alga, C. reinhardtii, using paramecium ... S. M. Miller (2010). "Volvox, Chlamydomonas, and the evolution of multicellularity". Nature Education. 3 (9): 65.. ... They found that in the presence of this predator, C. reinhardtii does indeed evolve simple multicellular features. ...
Eyespot-Assembly Mutants in Chlamydomonas reinhardtii. Mary Rose Lamb, Susan K. Dutcher, Cathy K. Worley and Carol L. Dieckmann ... Eyespot-Assembly Mutants in Chlamydomonas reinhardtii. Mary Rose Lamb, Susan K. Dutcher, Cathy K. Worley and Carol L. Dieckmann ... Eyespot-Assembly Mutants in Chlamydomonas reinhardtii. Mary Rose Lamb, Susan K. Dutcher, Cathy K. Worley and Carol L. Dieckmann ... Chlamydomonas reinhardtii is a single-celled green alga that phototaxes toward light by means of a light-sensitive organelle, ...
The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the ... Identification of nitrate transporter genes in Chlamydomonas reinhardtii Plant J. 1994 Mar;5(3):407-19. doi: 10.1111/j.1365- ... The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the ... These data strongly support the hypothesis that the nitrate transport system in C. reinhardtii contains at least two protein ...
Towards functional proteomics of membrane protein complexes: analysis of thylakoid membranes from Chlamydomonas reinhardtii.. ... approach to analyse photosynthetic thylakoid membranes isolated from wild-type and mutant strains of Chlamydomonas reinhardtii ...
We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a ... Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase ... Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase ... Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase ...
... and central metabolic pathways in the model unicellular green alga Chlamydomonas reinhardtii. The central role that microalgae ... Pérez-Pérez, M.E.; Crespo, J.L. Autophagy in the model alga Chlamydomonas reinhardtii. Autophagy 2010, 6, 562-563. [Google ... Lee, D.Y.; Fiehn, O. Metabolomic response of Chlamydomonas reinhardtii to the inhibition of target of rapamycin (TOR) by ... Oil accumulation in the model green alga Chlamydomonas reinhardtii: Characterization, variability between common laboratory ...
... eukaryotic green alga Chlamydomonas reinhardtii (hereafter called Chlamydomonas) is a venerable model system for plant biology ... The Chlamydomonas reinhardtii laboratory strains mcd4 [LS2] mt+ or mcd5 [LS6] mt+ and S1-D2 mt− (CC-2290) were used as parents ... In Chlamydomonas, most STS markers have one primer conserved in both C. reinhardtii and S1-D2 and one that is specific for each ... Li JB, Lin S, Jia H, Wu H, Roe BA, Kulp D, Stormo GD, Dutcher SK (2003) Analysis of Chlamydomonas reinhardtii genome structure ...
Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. Kaiyao Huang, Dennis R. Diener, Aaron Mitchell, Gregory J. ... Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. Kaiyao Huang, Dennis R. Diener, Aaron Mitchell, Gregory J ... Gametic differentiation in Chlamydomonas reinhardtii. I. Production of gametes and their fine structure. J. Cell Biol. 67:587- ... Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas reinhardtii. J. Cell Biol. 105:2279-2292. ...
We have shown that vegetative cells of C. reinhardtii are attracted by nitrite. The Nia1nit2 mutant with defects in genes ... reinhardtii during gamete formation: three protein kinases, one operating against signal flux and two that promote signal ... including Chlamydomonas reinhardtii. We have shown that vegetative cells of C. reinhardtii are attracted by nitrite. The ... Chlamydomonas reinhardtii strain CC-124 (nia1, nit2, mt−) have been described elsewhere [31]. Another wild-type strain tested ...
A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, ... Expression of a histidine-tagged RNA pyrophosphohydrolase in Chlamydomonas reinhardtii for localization studies. Aaløkken, ... A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii ... The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was ...
The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in ... The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in ... highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas ... highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas ...
3′ end maturation of the Chlamydomonas reinhardtii chloroplast atpB mRNA is a two-step process.Mol. Cell. Biol. 13 1993 2277 ... 3′-Processed mRNA Is Preferentially Translated in Chlamydomonas reinhardtii Chloroplasts. Ruth Rott, Haim Levy, Robert G. ... 3′-Processed mRNA Is Preferentially Translated in Chlamydomonas reinhardtii Chloroplasts. Ruth Rott, Haim Levy, Robert G. ... 3′-Processed mRNA Is Preferentially Translated in Chlamydomonas reinhardtii Chloroplasts. Ruth Rott, Haim Levy, Robert G. ...
The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP- ... Die Grünalge Chlamydomonas reinhardtii ist ein Modellorganismus für die Analyse der eukaryotischen Geißel. In dieser Arbeit ... Schoppmeier, Jutta (2004) GFP-Markierung von Cytoskelettproteinen in Chlamydomonas reinhardtii (Chlorophyceae). PhD thesis, ... Die Analyse von Chlamydomonas Mutanten zeigte, dass die Anordnung der GFP-SFA Polymere von der Basalkörperanzahl und der ...
In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta ... Carbonic Anhydrase and Carbonic Anhydrase like Genes of Chlamydomonas Reinhardtii. by Mitra, Mautusi ... A novel beta CA (Cah6) and a putative gamma CA (Gclp1) gene were identified in C. reinhardtii. Gclp1 is one of two putative ... This dissertation describes the identification and characterization of new CA genes from C. reinhardtii. Four new CA or CA like ...
When C. reinhardtii is exposed to aminophylline (theophylline(2) ethylenediamine), the concentration of the factor in the cells ... We conclude that cAMP occurs in C. reinhardtii and that the endogenous concentration is governed at least in part by a ... Cyclic nucleotide phosphodiesterase activity is present in crude extract of C. reinhardtii and is inhibited by theophylline. ... reinhardtii to the resultant elevation of endogenous cAMP. ... Chlamydomonas reinhardtii contains a factor that can replace ...
en] Photosynthetic activities were analyzed in Chlamydomonas reinhardtii mitochondrial mutants affected in different complexes ... Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration. ... The present results demonstrate that in C. reinhardtii mutants, permanent defects in the mitochondrial electron transport chain ...
Chlamydomonas reinhardtii cell, life cycle, strains, mating types Chlamydomonas reinhardtii at the Encyclopedia of Life Guiry, ... Chlamydomonas species are widely distributed worldwide in soil and fresh water. Chlamydomonas reinhardtii is an especially well ... Chlamydomonas reinhardtii resources The Chlamydomonas Center - genomic, genetic and bibliographic information and the ... and reinhardtii all refer to the same species, C. reinhardtii Dangeard. Chlamydomonas is used as a model organism for research ...
The D66 strain of Chlamydomonas reinhardtii, a single-celled green alga, is a cell-wall-deficient strain of algae that exhibits ... The D66 strain of Chlamydomonas reinhardtii, has been genetically engineered with no cell wall in order to increase the ... Adams, James (May 2004). "MOLECULAR, GENETIC AND PHYSIOLOGICAL CHARACTERIZATION OF A CHLAMYDOMONAS REINHARDTII INSERTIONAL ... Most wild-type Chlamydomonas strains can grow on fixed carbon sources (e.g., acetate) in the dark. It is possible that the D66 ...
High-frequency nuclear transformation of Chlamydomonas reinhardtii.. K L Kindle. PNAS February 1, 1990 87 (3) 1228-1232; https ... High-frequency nuclear transformation of Chlamydomonas reinhardtii. Message Subject (Your Name) has sent you a message from ... Gametogenesis in the Chlamydomonas reinhardtii minus Mating Type Is Controlled by Two Genes, MID and MTD1 ... By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass ...
Maul, JE, Lilly, JW, Cui, L, DePamphilis, CW, Miller, W, Harris, EH, Stern, DB 2002The Chlamydomonas reinhardtii Plastid ... Klein, U, De Camp, JD, Bogorad, L 1992Two types of chloroplast gene promoters in Chlamydomonas reinhardtiiProc Natl Acad Sci ... Van, K, Wang, Y, Nakamura, Y, Spalding, MH 2001Insertional mutants of Chlamydomonas reinhardtii that require elevated CO2 for ... Liu, XQ, Gillham, NW, Boynton, JE 1988Chloroplast ribosomal protein L-18 in Chlamydomonas reinhardtii is processed during ...
Boyle NR, Morgan JA (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3:4CrossRef ... Buleon A et al (1997) Starches from A to C. Chlamydomonas reinhardtii as a model microbial system to investigate the ... In this work we examined the possibility of studying, by solid-state NMR, the model organism Chlamydomonas reinhardtii fully ... Bonente G, Pippa S, Castellano S, Bassi R, Ballottari M (2012) Acclimation of Chlamydomonas reinhardtii to different growth ...
Chlamydomonas reinhardtii (Chlamydomonas smithii). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand% ... sp,B5BUZ8,KTU_CHLRE Protein kintoun OS=Chlamydomonas reinhardtii OX=3055 GN=pf13 PE=2 SV=1 ... "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.". Piperno G., ... "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.". Piperno G., ...
Hoshaw, R.W. & Ettl, H. (1966). Chlamydomonas smithii sp. nov. - a chlamydomonad interfertile with Chlamydomonas reinhardtii. ... Chlamydomonas reinhardtii P.A.Dangeard. Classification: Empire Eukaryota. Kingdom Plantae. Subkingdom Viridiplantae. ... Isolation and preliminary characterization of three Chlamydomonas strains interfertile with Chlamydomonas reinhardtii ( ... as Chlamydomonas reinhardtii P.A.Dangeard). Europe: Balearic Islands (Cambra Sánchez, Álvarez Cobelas & Aboal Sanjurjo 1998), ...
Chlamydomonas reinhardtii. Mutation(s): 0 Gene Names: Cre07.g324550, CHLRE_07g324550v5. Find proteins for B0LUZ5 (Chlamydomonas ... Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.. Burgess, S.J., Taha, H., Yeoman, J ... Identification of the pyruvate reductase of Chlamydomonas reinhardtii. *DOI: 10.2210/pdb4ZGS/pdb ... Under anoxic conditions the green alga Chlamydomonas reinhardtii activates various fermentation pathways leading to the ...
Genomics and functional genomics in Chlamydomonas reinhardtii. Title: Genomics and functional genomics in Chlamydomonas ... Here, in this paper, we employ the reference unicellular green alga Chlamydomonas reinhardtii to identify the effect of H 2O 2 ... Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing ... Upon fertilization Chlamydomonas reinhardtii zygotes undergo a program of differentiation into a diploid zygospore that is ...
Hoshaw, R.W. & Ettl, H. (1966). Chlamydomonas smithii sp. nov. - a chlamydomonad interfertile with Chlamydomonas reinhardtii. ... Chlamydomonas reinhardtii P.A.Dangeard 1888. Classification: Empire Eukaryota. Kingdom Plantae. Subkingdom Viridiplantae. ... Isolation and preliminary characterization of three Chlamydomonas strains interfertile with Chlamydomonas reinhardtii ( ... Ford, C. & Wang, W.-Y. (1980). Three new yellow loci in Chlamydomonas reinhardtii. Molecular and General Genetics 179: 259-264. ...
... Larisa ... "Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts," Plant And Cell Physiology, vol. 57 ... "High-Resolution Profiling of a Synchronized Diurnal Transcriptome from Chlamydomonas reinhardtii Reveals Continuous Cell and ...
Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library.. Purton S1, Rochaix JD. ... We report the rescue of an arginine-requiring mutant (arg7-8) of Chlamydomonas reinhardtii by complementation using total DNA ... We conclude that these represent true transformants and that any stable nuclear mutant of Chlamydomonas could be rescued using ...
Chlamydomonas reinhardtii (Chlamydomonas smithii). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_ ... sp,Q9XHH2,DNAL1_CHLRE Dynein light chain 1, axonemal OS=Chlamydomonas reinhardtii OX=3055 GN=LC1 PE=1 SV=1 ... "The Chlamydomonas genome reveals the evolution of key animal and plant functions.". Merchant S.S., Prochnik S.E., Vallon O., ... "Light chain 1 from the Chlamydomonas outer dynein arm is a leucine-rich repeat protein associated with the motor domain of the ...
Ubiquinone and other terpenoid-quinone biosynthesis - Chlamydomonas reinhardtii [ Pathway menu , Organism menu , Pathway entry ...
The biflagellate green alga Chlamydomonas reinhardtii exhibits both positive and negative phototaxis to inhabit areas with ... Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii Proc Natl Acad Sci U S A. 2016 May 10;113( ... The biflagellate green alga Chlamydomonas reinhardtii exhibits both positive and negative phototaxis to inhabit areas with ...
cDNA clones for the periplasmic carbonic anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells ... cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 ... cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 ... cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 ...
Overexpressing Ferredoxins in Chlamydomonas reinhardtii Increase Starch and Oil Yields and Enhance Electric Power Production in ... Linalool- and α-terpineol-induced programmed cell death in Chlamydomonas reinhardtii. Yueting Chen, Yuanyuan Weng, Min Zhou, ... Acetic acid-induced programmed cell death and release of volatile organic compounds in Chlamydomonas reinhardtii. Plant Physiol ... Photosynthetic electron flow affects H2O2 signaling by inactivation of catalase in Chlamydomonas reinhardtii. Planta 228: 1055- ...
Characterizing the Anaerobic Response of Chlamydomonas reinhardtii by Quantitative Proteomics. Mia Terashima, Michael Specht, ... Characterizing the Anaerobic Response of Chlamydomonas reinhardtii by Quantitative Proteomics. Mia Terashima, Michael Specht, ... 1989) Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardtii. Plant ... 1992) Characterization of chlorophyll a/b proteins of photosystem I from Chlamydomonas reinhardtii. J. Biol. Chem. 267, 25714- ...
Cells of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission ... Chlamydomonas reinhardtii Cell Line. ac-5 ac-31 Cellular Component. chloroplast plastid plastid thylakoid membrane ... Cells of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission ... Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38707, Chlamydomonas reinhardtii. CIL. Dataset. https://doi.org/doi: ...
Chlamydomonas reinhardtii. Mutation(s): 1 Gene Names: rbcL. EC: 4.1.1.39. Find proteins for P00877 (Chlamydomonas reinhardtii) ... Chlamydomonas reinhardtii. Mutation(s): 0 Gene Names: RBCS-1, CHLRE_02g120100v5, CHLREDRAFT_82986. EC: 4.1.1.39. ... Crystal structure of Chlamydomonas reinhardtii Rubisco with a large- subunit supressor mutation T342I. *DOI: 10.2210/pdb2V67/ ... Genetic screening in Chlamydomonas reinhardtii previously identified a loop-6 V331A substitution that decreases carboxylation ...
Kathir, P., M. Lavoie, W. J. Brazelton, N. A. Haas, P. A. Lefebvre et al., 2003 Molecular map of the Chlamydomonas reinhardtii ... Porter, M. E., J. A. Knott, S. H. Myster and S. J. Farlow, 1996 The dynein gene family in Chlamydomonas reinhardtii. Genetics ... Nelson, J. A., P. B. Savereide and P. A. Lefebvre, 1994 The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a ... Barsel, S.-E., D. E. Wexler and P. A. Lefebvre, 1988 Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. ...
The Chlamydomonas reinhardtii Plastid Chromosome. Jude E. Maul, Jason W. Lilly, Liying Cui, Claude W. dePamphilis, Webb Miller ... The Chlamydomonas reinhardtii Plastid Chromosome. Jude E. Maul, Jason W. Lilly, Liying Cui, Claude W. dePamphilis, Webb Miller ... The Chlamydomonas reinhardtii Plastid Chromosome. Islands of Genes in a Sea of Repeats. Jude E. Maul, Jason W. Lilly, Liying ... Chlamydomonas reinhardtii is a unicellular eukaryotic alga possessing a single chloroplast that is widely used as a model ...
The Chlamydomonas reinhardtii Plastid Chromosome. Jude E. Maul, Jason W. Lilly, Liying Cui, Claude W. dePamphilis, Webb Miller ... The Chlamydomonas reinhardtii Plastid Chromosome. Jude E. Maul, Jason W. Lilly, Liying Cui, Claude W. dePamphilis, Webb Miller ... The Chlamydomonas reinhardtii Plastid Chromosome. Islands of Genes in a Sea of Repeats. Jude E. Maul, Jason W. Lilly, Liying ... Conserved gene clusters in the highly rearranged chloroplast genomes of Chlamydomonas moewusii and Chlamydomonas reinhardtii. ...
... and central metabolic pathways in the model unicellular green alga Chlamydomonas reinhardtii. The central role that microalgae ...
  • A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. (uio.no)
  • Deletion of the Chlamydomonas chloroplast atpB 3′ IR in strain Δ26 results in reduced accumulation of atpB transcripts and the chloroplast ATPase β-subunit, leading to weakly photosynthetic growth. (asm.org)
  • The ability to introduce altered genes into the chloroplast of the green alga C. reinhardtii presented the opportunity to test the in vitro results in an in vivo context. (asm.org)
  • In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. (elsevier.com)
  • The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. (frontiersin.org)
  • The unicellular green alga Chlamydomonas reinhardtii is an ideal model organism for studies of ciliary function and assembly. (ku.edu)
  • The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the nitrate reductase structural gene nit-1, have been related to nitrate transport. (nih.gov)
  • These data strongly support the hypothesis that the nitrate transport system in C. reinhardtii contains at least two protein components encoded by the nar-2 and nar-3 genes. (nih.gov)
  • The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. (umassmed.edu)
  • With the elucidation of the Chlamydomonas genome, bioinformatics analysis has allowed us to determine potential genes involved in ergosterol biosynthesis. (lsu.edu)
  • Also Chlamydomonas strains lacking flagella were analyzed by Q-RT PCR to determine what role ergosterol biosynthetic genes played in the absence of their flagella. (lsu.edu)
  • iologists at UC San Diego have announced the successful engineering of Chlamydomonas reinhardtii , a green alga commonly used in laboratories, into a rainbow of different colors by producing six different colored fluorescent proteins in the algae cells. (algaeindustrymagazine.com)
  • Towards functional proteomics of membrane protein complexes: analysis of thylakoid membranes from Chlamydomonas reinhardtii. (nih.gov)
  • In addition a protein activity assay was developed in order to confirm that the Chlamydomonas RppH homolog has RNA pyrophosphohydrolase activity. (uio.no)
  • Chlamydomonas reinhardtii contains a factor that can replace adenosine 3':5'-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. (semanticscholar.org)
  • The flagellar motility of Chlamydomonas pf25 mutant lacking an AKAP-binding protein is overtly sensitive to medium conditions. (semanticscholar.org)
  • Projects in the lab are focused on improving our understanding of photosynthesis and metabolism in Chlamydomonas reinhardtii (model alga). (carnegiescience.edu)
  • Using cross validation, permutation and rank products statistical procedures we identified biomarker regions in proton spectra of polar metabolites extracted from the algae Chlamydomonas reinhardtii (light and dark grown) and from the yeast Saccharomyces cerevisae (aerobically and anaerobically grown). (technologynetworks.com)
  • A very similar sequence, located ∼60 nucleotides upstream of the atpI initiation codon, was also found in some Chlorophyceae and Ulvale algae species and is essential for atpI mRNA translation in Chlamydomonas. (elsevier.com)
  • Several months ago, biologists in the same UC San Diego laboratory reported genetically engineering Chlamydomonas algae to produce a complex and expensive human therapeutic drug used to treat cancer. (algaeindustrymagazine.com)
  • Adenosine 3':5'-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization. (semanticscholar.org)
  • Characterization of THB1, a Chlamydomonas reinhardtii truncated hemogl" by Eric A. Johnson, Selena L. Rice et al. (umassmed.edu)
  • Results from this dissertation provides the groundwork for future experimentation in the field of sterol lipid research in Chlamydomonas reinhardtii. (lsu.edu)
  • In this study we undertook such an approach to analyse photosynthetic thylakoid membranes isolated from wild-type and mutant strains of Chlamydomonas reinhardtii. (nih.gov)
  • While past studies have identified some ergosterol mutants in C. reinhardtii, very little is known about sterol biosynthesis pathways in this species. (lsu.edu)
  • These erg3 null mutants were transformed with a vector expressing ERG3 cDNA from C. reinhardtii driven by the yeast ADH1 promoter, and this expression was able to restore ergosterol biosynthesis and reverse phenotypes associated with lack of ERG3 function. (lsu.edu)
  • For example, 5′ ends are often formed by endonucleolytic processing of primary transcripts, and this may be the exclusive mode of 5′ end formation in chloroplasts in the green alga Chlamydomonas reinhardtii (reviewed in reference 12 ). (asm.org)
  • When challenged with excess light, the green alga Chlamydomonas reinhardtii responds, in part, by down-regulating light harvesting capacity at Photosystem II while concomitantly reorganising cellular metabolism to increase sink capacity. (dundee.ac.uk)
  • We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. (edu.au)
  • By minimizing flagellar length variability using a simple method requiring only hours and no changes in media, flagellar synchronization facilitates the detection of small changes in flagellar length resulting from both chemical and genetic perturbations in Chlamydomonas. (ku.edu)
  • Chlamydomonas reinhardtii is an algal model system for studying mammalian cilium formation and function. (ku.edu)
  • By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is linked to the nitrogen assimilation pathway. (umassmed.edu)
  • To test whether ERG3 of C. reinhardtii had a similar function, yeast Saccharomyces cerevisiae ERG3 knockout strains were created to assess whether a plasmid expressing the Chlamydomonas ERG3 could complement the deletion. (lsu.edu)
  • Unlike most trans-acting factors characterized so far in Chlamydomonas, which control the expression of a single gene, MTHI1 targets two distinct transcripts: it is required for the accumulation and translation of atpH mRNA, encoding a subunit of the selective proton channel, but it also enhances the translation of atpI mRNA, which encodes the other subunit of the channel. (elsevier.com)
  • In addition, the nucleotide sequence of the 3′ untranslated regions (UTRs) can influence the accumulation of a correctly 3′-end-processed transcript, since the functionality of some Chlamydomonas 3′ IRs is orientation dependent in vivo ( 5 , 37 ). (asm.org)
  • A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. (uio.no)
  • We present example data sets from mammalian neural tissue, Drosophila brain, and Chlamydomonas reinhardtii to illustrate the power of this novel high-resolution technique to address questions in both connectomics and cell biology. (janelia.org)
  • The phenotypical and physiological implications revealed from our experiments highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas under photo-oxidative stress conditions. (frontiersin.org)
  • The findings based on the studies of the mutants have improved our understanding of cell response in Chlamydomonas to oxidative stress substantially. (frontiersin.org)
  • Complementation of these erg3 null phenotypes strongly suggests that ERG3 in C. reinhardtii functions as a sterol C-5 desaturase. (lsu.edu)
  • The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. (uio.no)
  • Genetic and biochemical studies have revealed that chloroplast gene expression in Chlamydomonas is controlled primarily post-transcriptionally, including events that effect mRNA processing and stability, and during the translation of plastid mRNAs into proteins. (springer.com)
  • Emergence of the draft sequence of the Chlamydomonas nuclear genome has enabled us to carry out a prediction and comparative analysis of the proteins required for chloroplast mRNA translation. (springer.com)
  • Chlamydomonas chloroplasts contain all of the general translation factors found in bacteria, and a majority of the ribosomal proteins are conserved between plastids and bacteria. (springer.com)
  • However, Chlamydomonas contains a number of additional proteins and protein domains associated with the plastid ribosome, while some ribosomal proteins are either quite divergent or lacking. (springer.com)
  • As we continue to characterize and understand biological processes in C. reinhardtii and translate that knowledge to other systems, we are faced with the realization that many genes encode proteins without a defined function. (osti.gov)
  • To identify proteins involved during anaerobic acclimation as well as to localize proteins and pathways to the powerhouses of the cell, chloroplasts and mitochondria from C. reinhardtii in aerobic and anaerobic (induced by 8 h of argon bubbling) conditions were isolated and analyzed using comparative proteomics. (mcponline.org)
  • Qualitative and semiquantitative analyses of isolated chloroplasts and mitochondria from aerobic and anaerobic C. reinhardtii cultures allowed for the identification and localization of proteins, including a handful of fermentative proteins. (mcponline.org)
  • Another important feature of C. reinhardtii is that it has the capacity to grow with light as a sole energy source (photoautotrophic growth) or on acetate in the dark (heterotrophically), facilitating detailed examination of genes and proteins critical for photosynthetic or respiratory function. (asm.org)
  • In this study, we established detailed two-dimensional protein maps of Chlamydomonas reinhardtii light-harvesting proteins (Lhca and Lhcb) by extensive tandem mass spectrometric analysis. (asm.org)
  • This proteomic study demonstrates the complexity of the light-harvesting proteins at the protein level in C. reinhardtii and will be an important basis of future functional studies addressing this diversity. (asm.org)
  • Separation of isolated PSI complexes from C. reinhardtii by two-dimensional gel electrophoresis has revealed the presence of about 18 LHCI protein (LHC protein of PSI) spots, thereby suggesting an even more extensive variability of Lhca proteins ( 20 ). (asm.org)
  • To characterize the compositions of the lhca and lhcb gene products at the protein level and to monitor the posttranslational modifications of LHC proteins of C. reinhardtii , we performed a detailed proteomic study. (asm.org)
  • In Chlamydomonas reinhardtii microtubules and associated proteins are added simultaneously. (elifesciences.org)
  • Sarah Zhang writes in Wired Magazine that the single-cell green algae Chlamydomonas reinhardtii have an eyespot that makes use of light-sensitive proteins. (algaeindustrymagazine.com)
  • The GAPDH, CP12 and PRK recombinant proteins are able to reconstitute spontaneously the ternary complex that has been described in Chlamydomonas reinhardtii. (sigmaaldrich.com)
  • however, the Chlamydomonas genome encodes presumed plant orthologs of a chloroplast lipid transporter consisting of TGD (TRIGALACTOSYLDIACYLGLYCEROL) proteins that are required for ER-tochloroplast lipid trafficking in plants. (unl.edu)
  • Our experiments thus provide extensive evidence that FtsH plays a major role in the quality control of thylakoid membrane proteins and in the response of C. reinhardtii to light and macronutrient stress. (diva-portal.org)
  • This improved efficiency should greatly facilitate a variety of genetic and cell-biological studies in Chlamydomonas and also enable new applications such as expression-based screens and large-scale production of foreign proteins. (g3journal.org)
  • These problems have handicapped cell biological studies and impeded development of expression-based screens, medium- to high-throughput imaging analyses, and the use of Chlamydomonas as a host for expression of foreign proteins. (g3journal.org)
  • We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. (asm.org)
  • Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. (bmmj.org)
  • Conclusions: It is suggested that the expression system optimized by this study can potentially be used for the production of important therapeutic proteins and other heterologous proteins in C. reinhardtii. (bmmj.org)
  • Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5′ untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii. (concordia.ca)
  • Clemetson, JM, A Boschetti, KJ Clemetson(1992) Chloroplast envelope proteins are encoded by the chloroplast genome of Chlamydomonas reinhardtii. (concordia.ca)
  • Lee, H, Bingham, SE & Webber, AN 1998, ' Specific mutagenesis of reaction center proteins by chloroplast transformation of Chlamydomonas reinhardtii ', Methods in Enzymology , vol. 297, pp. 310-320. (elsevier.com)
  • We identified by mass spectrometry 682 heat-resistant proteins from the green alga, Chlamydomonas reinhardtii. (archives-ouvertes.fr)
  • Under anoxic conditions the green alga Chlamydomonas reinhardtii activates various fermentation pathways leading to the creation of formate, acetate, ethanol and small amounts of other metabolites including d-lactate and hydrogen. (rcsb.org)
  • The biflagellate green alga Chlamydomonas reinhardtii exhibits both positive and negative phototaxis to inhabit areas with proper light conditions. (nih.gov)
  • The versatile metabolism of the green alga Chlamydomonas reinhardtii is reflected in its complex response to anaerobic conditions. (mcponline.org)
  • Cells of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. (cellimagelibrary.org)
  • We have cloned a gene ( Mut6 ) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. (sciencemag.org)
  • Sometimes called green yeast ( Goodenough, 1992 ), the unicellular, eukaryotic green alga Chlamydomonas reinhardtii (hereafter called Chlamydomonas) is a venerable model system for plant biology as well as for cell motility. (plantphysiol.org)
  • The single chloroplast of the unicellular alga Chlamydomonas reinhardtii contains an orange carotenoid pigment spot that functions in phototaxis. (cellimagelibrary.org)
  • Here we report a detailed characterization of the remodeling of photosynthesis upon sulfur starvation under heterotrophy and photo-autotrophy in the green alga ( Chlamydomonas reinhardtii ). (plantphysiol.org)
  • Isolated chloroplasts of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. (ucsd.edu)
  • The unicellular green alga Chlamydomonas reinhardtii is capable of producing H 2 by splitting water with energy from sunlight. (rsc.org)
  • A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. (uio.no)
  • The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. (frontiersin.org)
  • 2D measurement of ion currents associated to the signal transduction of the phototactic alga Chlamydomonas reinhardtii. (biomedsearch.com)
  • For example, 5′ ends are often formed by endonucleolytic processing of primary transcripts, and this may be the exclusive mode of 5′ end formation in chloroplasts in the green alga Chlamydomonas reinhardtii (reviewed in reference 12 ). (asm.org)
  • The work describes a novel approach for sustained photobiological production of H2 gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii. (oilgae.com)
  • To produce energy for cell growth, the green alga Chlamydomonas reinhardtii possesses the metabolic flexibility to use light and/or carbon sources such as acetate. (mcponline.org)
  • The time course of and the influence of light intensity and light quality on the induction of a mitochondrial carbonic anhydrase (CA) in the unicellular green alga Chlamydomonas reinhardtii was characterized using western and northern blots. (diva-portal.org)
  • In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta CAs. (openthesis.org)
  • We used a mass spectrometry-based approach to achieve a comprehensive mapping of the in vivo protein phosphorylation sites within photosynthetic membranes from the green alga Chlamydomonas reinhardtii subjected to distinct environmental conditions known to affect the photosynthetic machinery. (diva-portal.org)
  • The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology conserved throughout the eukaryotic phylogeny. (g3journal.org)
  • By using principles of synthetic biology, we have constructed a platform to characterize regulatory properties of miRNAs in the model alga Chlamydomonas reinhardtii. (cam.ac.uk)
  • Lefebvre, Paul 2004-10-06 00:00:00 A new transposable element, Tcr3, was identified in the unicellular green alga Chlamydomonas reinhardtii. (deepdyve.com)
  • New factors regulating magnesium chelatase in the green alga Chlamydomonas reinhardtii Chekunova, E. (deepdyve.com)
  • The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. (whiterose.ac.uk)
  • The unicellular green alga Chlamydomonas reinhardtii has emerged to be an important model organism for the study of oxygenic eukaryotic photosynthesis as well as other processes occurring in the chloroplast. (semanticscholar.org)
  • With an eye to circumventing these problems in the future and engineering the robust alcohol-producing microalgal hosts, we investigated the metabolic responses of the model green alga Chlamydomonas reinhardtii to ethanol and butanol. (biomedcentral.com)
  • The green alga Chlamydomonas reinhardtii has been established as a model organism for fundamental biological research during the last decades. (uni-regensburg.de)
  • Results Here, the genomes of the green alga Chlamydomonas reinhardtii, the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii and the seed plant Arabidopsis thaliana were screened for ORFs encoding chloroplast peroxidases. (fu-berlin.de)
  • The unicellular green alga Chlamydomonas reinhardtii responds to sulfate deprivation by producing an arylsulfatase (Lien, T., and O. Schreiner. (rupress.org)
  • The biflagellate green alga Chlamydomonas reinhardtii is an ideal genetic model for the integration of the effects on Li + on signal transduction, gene expression, and aspects of flagellar biogenesis. (elsevier.com)
  • The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. (biomedcentral.com)
  • The supply of inorganic carbon (Ci) at the site of fixation by Rubisco is a key parameter for efficient CO2 fixation in aquatic organisms including the green alga, Chlamydomonas reinhardtii. (illinois.edu)
  • We identified a hypothetical protein in the eukaryotic green alga Chlamydomonas reinhardtii , which encodes a protein containing an a ntibiotic b iosynthesis m onooxygenase (ABM) domain consistent with those associated with IsdG family members. (asm.org)
  • IMPORTANCE This work establishes a protein in the freshwater alga Chlamydomonas reinhardtii as an IsdG family heme oxygenase. (asm.org)
  • The nitrate reductase gene from wild-type Chlamydomonas was used to complement a mutation in the corresponding gene of a strain containing nit1-305. (pnas.org)
  • This high frequency of cotransformation will allow any cloned gene to be introduced into Chlamydomonas. (pnas.org)
  • The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the nitrate reductase structural gene nit-1, have been related to nitrate transport. (nih.gov)
  • Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta) to facilitate gene function analysis. (algaebase.org)
  • Phylogenetic reconstruction of changes in plastid genome content revealed that an accelerated rate of gene loss also characterized the Chlamydomonas/Chlorella lineage, a phenomenon that might be independent of the proliferation of SDRs. (plantcell.org)
  • Tools that have not been previously applied to Chlamydomonas, such as bulked segregant analysis and marker duplexing, are being implemented to increase the speed at which one can go from mutant phenotype to gene. (plantphysiol.org)
  • To analyze the function of ciliary polycystic kidney disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned the gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with the characteristics of PKD2 family members. (rupress.org)
  • At least nine different lhca -related gene products were predicted by comparison of the mass spectrometric data against Chlamydomonas expressed sequence tag and genomic databases, demonstrating the extensive variability of the C. reinhardtii Lhca antenna system. (asm.org)
  • To resolve this conundrum, we identified a mutant of Chlamydomonas deleted in the TGD2 gene and characterized the respective protein, CrTGD2. (unl.edu)
  • A novel beta CA (Cah6) and a putative gamma CA (Gclp1) gene were identified in C. reinhardtii. (openthesis.org)
  • A heat inducible expression vector containing amiRNA targeting OEE2 gene was constructed and transformed into Chlamydomonas reinhardtii . (oilgae.com)
  • The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. (asm.org)
  • With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii. (asm.org)
  • To investigate how gene families and gene expression have evolved, particularly in the context of stress response that have been shown to correlate with gene family expansion in multiple eukaryotes, we characterized the expansion patterns of gene families in nine green algal species, and examined evolution of stress response among gene duplicates in Chlamydomonas reinhardtii . (biomedcentral.com)
  • The nuclear gene encoding the PsaN subunit from C. reinhardtii was cloned and characterised. (ucl.ac.uk)
  • Ghanbari Motlagh, M., Amini-Bayat, Z., Ofoghi, H. Investigation of an Optimized Context for the Expression of GFP as a Reporter Gene in Chlamydomonas Reinhardtii. (bmmj.org)
  • The genome sequence and gene models of Chlamydomonas reinhardtii were downloaded from Phytozome , the Joint Genome Institute (JGI) plant genomics portal. (doe.gov)
  • By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is linked to the nitrogen assimilation pathway. (umassmed.edu)
  • An oligonucleotide of a cabII gene, derived from a highly conserved region, was used to identify potential cab gene regions in the nuclear genome of Chlamydomonas. (uni-bielefeld.de)
  • British Library EThOS: Glycoproteins of the cell wall of Chlamydomonas reinhardtii. (bl.uk)
  • Glycoproteins of the cell wall of Chlamydomonas reinhardtii. (bl.uk)
  • N. F. Wilson and P. A. Lefebvre, abstract presented at the 10th International Chlamydomonas Conference, 2002). (asm.org)
  • In this report, we focus on mutants affecting photosynthesis, in keeping with the thrust of this journal, and the emphasis of the newly renewed and National Science Foundation-supported Chlamydomonas genome project ( http://www.chlamy.org/ ). (plantphysiol.org)
  • The findings based on the studies of the mutants have improved our understanding of cell response in Chlamydomonas to oxidative stress substantially. (frontiersin.org)
  • The correct number and arrangement of the fibers depended on the number of basal bodies and the amount of centrin as revealed by analysis of C. reinhardtii mutants. (uni-koeln.de)
  • Reference : Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinha. (ac.be)
  • The present results demonstrate that in C. reinhardtii mutants, permanent defects in the mitochondrial electron transport chain stabilize state 2, which favors cyclic over linear electron transport in the chloroplast. (ac.be)
  • A series of conditional mutants of the algal, biflagellate Chlamydomonas reinhardtii with temperature-sensitive defects in flagellar assembly and function were isolated. (rupress.org)
  • Vila M, Díaz-Santos E, de la Vega M, Couso I and León R. Isolation and characterization of pigment deficient insertional mutants in the chlorophyte Chlamydomonas reinhardtii . (hoajonline.com)
  • While past studies have identified some ergosterol mutants in C. reinhardtii, very little is known about sterol biosynthesis pathways in this species. (lsu.edu)
  • These erg3 null mutants were transformed with a vector expressing ERG3 cDNA from C. reinhardtii driven by the yeast ADH1 promoter, and this expression was able to restore ergosterol biosynthesis and reverse phenotypes associated with lack of ERG3 function. (lsu.edu)
  • We have found that the model unicellular green algae Chlamydomonas reinhardtii forms aggregates in response to the presence of the filter feeding zooplanktonic predator, Daphnia magna. (k-state.edu)
  • In addition, Chlamydomonas chloroplasts contain a number of mRNA specific translation factors that are not found in bacteria. (springer.com)
  • When mating type plus and minus gametes of Chlamydomonas are mixed, they agglutinate with each other via their flagella, fuse, then initiate the zygote formation program which includes synthesis of the zygote cell wall, fusion of nuclei and chloroplasts, and the digestion of chloroplast DNA from the minus parent. (oup.com)
  • Gallagher, Daniel, "Ubiquitin in the chloroplasts of Chlamydomonas reinhardtii" (1996). (richmond.edu)
  • cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration. (pnas.org)
  • The cultivation of Chlamydomonas reinhardtii (a unicellular biflagellate fresh-water microalga) is modeled as a three-step chemical mechanism representing growth, respiration, and lipid production. (nebraska.edu)
  • Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtii. (rupress.org)
  • Chlamydomonas reinhardtii is a single-celled green alga that phototaxes toward light by means of a light-sensitive organelle, the eyespot. (genetics.org)
  • CHLAMYDOMONAS reinhardtii is a phototactic, single-celled green alga. (genetics.org)
  • Some species of eukaryote-such as Chlamydomonas reinhardtii , a single-celled green alga, and Trypanosoma brucei , the protist parasite that causes African sleeping sickness-must grow new flagella when their cells divide, so that each new cell can swim. (elifesciences.org)
  • There has been an array of studies that have investigated C. reinhardtii under anaerobic conditions and provided valuable insights into the metabolic changes undertaken by the cell to acclimate to an anaerobic condition. (mcponline.org)
  • It is now well established that under anaerobic conditions C. reinhardtii induces a wide range of fermentative pyruvate-dependent metabolic pathways ( 11 ⇓ - 13 ). (mcponline.org)
  • Central carbon metabolism and electron transport in Chlamydomonas reinhardtii: metabolic constraints for carbon partitioning between oil and starch. (semanticscholar.org)
  • The study is the first comprehensive view of the metabolic mechanisms employed by C. reinhardtii to defend against ethanol or butanol toxicity. (biomedcentral.com)
  • In Chiamydomonas reinhardtii, K⁺ influx appears to be mediated by two discrete transport systems, a high affinity transport system (HATS) at low external [K⁺] and a low affinity transport system (LATS) at high external [K⁺]. These two transport systems were further characterized by employing various metabolic inhibitors, a K⁺ channel blocker and a sulfhydryl reagent. (ubc.ca)
  • Our working hypothesis is that the siRNA signal transmitted to Chlamydomonas has the same effect on the mRNA as the physical siRNA. (scirp.org)
  • Here we present the optimization of the siRNA signal transduction to Chlamydomonas while we measure the growth rate and then fine tune the conditions measuring the amount of mRNA produced under the influence of the signal specific for the mRNA. (scirp.org)
  • Chronological transition of mitochondrial morphology in Chlamydomonas reinhardtii (Chlorophyceae) poststationary phase growth. (algaebase.org)
  • Morphological changes in mitochondrial and chloroplast nucleoids and mitochondria during the Chlamydomonas reinhardtii (Chlorophyceae) cell cycle. (algaebase.org)
  • The Antarctic psychrophile, Chlamydomonas raudensis Ettl (UWO241) (Chlorophyceae, Chlorophyta) exhibits a limited capacity to photoacclimate to red light. (algaebase.org)
  • By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. (pnas.org)
  • Chlamydomonas cells rotate twice per second as they breaststroke forward, scanning the environment perpendicular to the swimming path much as a radar antenna scans the sky. (genetics.org)
  • carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells were isolated and characterized. (pnas.org)
  • Chlamydomonas cells actively maintain two flagella of equal length. (genetics.org)
  • Nitrite plays an important role in the nitrogen metabolism of most cells, including Chlamydomonas reinhardtii. (mdpi.com)
  • We have shown that vegetative cells of C. reinhardtii are attracted by nitrite. (mdpi.com)
  • One of these phenomena, the differentiation of vegetative cells into sexually mature cells has been widely studied in Chlamydomonas and may proceed in two steps [ 10 ]. (mdpi.com)
  • The analysis of the detected traces revealed two main vectorial components of the signal by the help of singular value decomposition (SVD), in concert with previous experimental findings and theoretical considerations suggesting different origins of the "fast" and "slow" components of the photoelectric response of Chlamydomonas and Haematococcus cells. (biomedsearch.com)
  • IFN-œ2a expression cassette was transferred to Chlamydomonas reinhardtii cells via Agrobacterium -mediated transformation method. (springer.com)
  • Chlamydomonas reinhardtii algae cells successfully grew and divided under exposure to both the blue laser, red laser and that of white light LED when each was applied individually or combined in a sequence. (brunel.ac.uk)
  • These results indicate that pungent compounds such as capsaicin and gingerol induce loss of flagellar motility and flagellar detachment in C . reinhardtii cells. (biologists.org)
  • Addition of acetate to a suspension of Chlamydomonas reinhardtii cells in darkness induced transient and biphasic non-photochemical quenching of Chl fluorescence (qN) due to ApH-dependent down-regulation of PSII and the transition from state 1 to state 2. (oup.com)
  • Chlamydomonas reinhardtii cells, when grown on limiting CO2, have a CO2-concentrating mechanism (CCM) that functions to concentrate CO2 at the site of Rubisco. (illinois.edu)
  • Among phototrophs, the unicellular green microalga Chlamydomonas reinhardtii is widely known as one of the best established model organisms. (uni-bielefeld.de)
  • However, the chloroplast proteome in C. reinhardtii has only recently been comprehensively characterized, made possible by proteomics emerging as an accessible and powerful tool over the last decade. (semanticscholar.org)
  • Chlamydomonas reinhardtii is an excellent model system for plant biologists because of its ease of manipulation, facile genetics, and the ability to transform the nuclear, chloroplast, and mitochondrial genomes. (plantphysiol.org)
  • The novel technique was based on the light gradient method (LGM), and the model object was Chlamydomonas reinhardtii, a phototactic unicellular alga, ideal for such experiments. (biomedsearch.com)
  • The relative adaptability and quick generation time have made Chlamydomonas an important model for biological research. (doe.gov)
  • The results are consistent with the model that CIA8 is involved in Ci uptake in C. reinhardtii. (illinois.edu)
  • Chlamydomonas reinhardtii serves as a very useful model system for the study of eukaryotic organisms. (richmond.edu)
  • The unicellular green alga C. reinhardtii is amenable to a diversity of genetic and molecular manipulations. (asm.org)
  • Recent years have seen the development of a molecular toolkit for C. reinhardtii ( 42 , 44 , 66 , 98 , 99 ). (asm.org)
  • Although many tools are available to facilitate genetic, molecular biological, biochemical, and cell biological studies in Chlamydomonas , expression of unselected transgenes of interest (GOIs) has been challenging. (g3journal.org)
  • The availability of the Chlamydomonas reinhardtii nuclear genome sequence continues to enable researchers to address biological questions relevant to algae, land plants and animals in unprecedented ways. (osti.gov)
  • article{osti_1351725, title = {Genomics and functional genomics in Chlamydomonas reinhardtii}, author = {Blaby, Ian K. and Blaby-Haas, Crysten E.}, abstractNote = {The availability of the Chlamydomonas reinhardtii nuclear genome sequence continues to enable researchers to address biological questions relevant to algae, land plants and animals in unprecedented ways. (osti.gov)
  • The program MultiPipMaker was used to compare the genic complement of Chlamydomonas with those of other chloroplast genomes and to scan the genomes for sequence similarities and repetitive DNAs. (plantcell.org)
  • Cytotoxicity and cell apoptosis assays involving the usage of the recombinant C. reinhardtii IFN-œ2a (Cr. (springer.com)
  • Then the recombinant vector was transformed into the nucleus of C. reinhardtii for expression. (bmmj.org)
  • High-frequency nuclear transformation of Chlamydomonas reinhardtii. (pnas.org)
  • A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. (uio.no)
  • Ergosterol is the major sterol found in the membranes of Chlamydomonas reinhardtii. (lsu.edu)
  • Averina, N. 2014-03-07 00:00:00 The unicellular green alga Chamydomonas reinhardtii, as distinct from higher plants synthesizing chlorophyll (Chl) only in the light, produces it also in darkness. (deepdyve.com)
  • Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. (rupress.org)
  • Genetic screening in Chlamydomonas reinhardtii previously identified a loop-6 V331A substitution that decreases carboxylation and CO2/O2 specificity. (rcsb.org)
  • CP12 residues involved in the formation and regulation of the glyceraldehyde-3-phosphate dehydrogenase-CP12-phosphoribulokinase complex in Chlamydomonas reinhardtii. (sigmaaldrich.com)
  • Chlamydomonas gametes fuse to form a temporary dikaryon cell with four flagella. (genetics.org)
  • Li + causes C. reinhardtii flagella to elongate to ∼1.4 times their normal length and blocks flagellar motility (S. Nakamura, H. Tabino, and M. K. Kojima, Cell Struct. (elsevier.com)