AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Chlamydomonas reinhardtiiChlamydomonasAlgal ProteinsChloroplastsFlagellaRNA, AlgalCytochromes fDNA, AlgalPhotosynthesisChlorophytaCytochrome b6f ComplexPhotosystem II Protein ComplexChlorophyllLightPhotosystem I Protein ComplexMolecular Sequence DataPhotosynthetic Reaction Center Complex ProteinsPlant ProteinsGenes, ProtozoanPlastocyaninThylakoidsAxonemeLight-Harvesting Protein ComplexesProtozoan ProteinsAmino Acid SequenceMutationArylsulfatasesDiuronDyneinsTransformation, GeneticAmylopectinPlastoquinoneVolvoxRibulose-Bisphosphate CarboxylaseDarknessSulfurCytochromes c6MicroalgaeRNA, ChloroplastBase SequenceSequence Homology, Amino AcidChloroplast ProteinsDNA, ChloroplastHydrogenaseCarbonic AnhydrasesCytochrome b GroupElectron TransportMalate Dehydrogenase (NADP+)Genetic Complementation TestCarbon DioxideStarchHydrogenCloning, MolecularCytochromesGenes, PlantChlorellaStarch SynthaseGeranylgeranyl-Diphosphate GeranylgeranyltransferaseOrganelle SizeFerredoxinsOxidation-ReductionSequence AlignmentRNA, ProtozoanPhylogenyKineticsMutagenesis, InsertionalMicrotubulesPhototrophic ProcessesIsoamylasePyruvate SynthaseGene Expression Regulation, PlantElectrophoresis, Polyacrylamide GelPhenotypeFluorescenceOxygenThioredoxin hRNA, MessengerCoproporphyrinogen OxidaseCiliaDNA, ProtozoanEukaryotaGenome, ChloroplastMolecular WeightIron-Sulfur ProteinsAnaerobiosisDNA, ComplementaryGenome, ProtozoanCentriolesTubulinMicroscopy, Electronbeta-TocopherolSpectinomycinCrosses, GeneticPlastidsRNA, PlantPhotophosphorylationCarbon
Chlamydomonas reinhardtii
A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.
Chlamydomonas
A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.
Algal Proteins
Proteins found in any species of algae.
Chloroplasts
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
Flagella
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
RNA, Algal
Ribonucleic acid in algae having regulatory and catalytic roles as well as involvement in protein synthesis.
Cytochromes f
Cytochromes f are found as components of the CYTOCHROME B6F COMPLEX. They play important role in the transfer of electrons from PHOTOSYSTEM I to PHOTOSYSTEM II.
DNA, Algal
Deoxyribonucleic acid that makes up the genetic material of algae.
Photosynthesis
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Chlorophyta
A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.
Cytochrome b6f Complex
A protein complex that includes CYTOCHROME B6 and CYTOCHROME F. It is found in the THYLAKOID MEMBRANE and plays an important role in process of PHOTOSYNTHESIS by transferring electrons from PLASTOQUINONE to PLASTOCYANIN or CYTOCHROME C6. The transfer of electrons is coupled to the transport of PROTONS across the membrane.
Photosystem II Protein Complex
A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.
Chlorophyll
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
Light
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
Photosystem I Protein Complex
A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Photosynthetic Reaction Center Complex Proteins
Protein complexes that take part in the process of PHOTOSYNTHESIS. They are located within the THYLAKOID MEMBRANES of plant CHLOROPLASTS and a variety of structures in more primitive organisms. There are two major complexes involved in the photosynthetic process called PHOTOSYSTEM I and PHOTOSYSTEM II.
Plant Proteins
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Genes, Protozoan
The functional hereditary units of protozoa.
Plastocyanin
A copper-containing plant protein that is a fundamental link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
Thylakoids
Membranous cisternae of the CHLOROPLAST containing photosynthetic pigments, reaction centers, and the electron-transport chain. Each thylakoid consists of a flattened sac of membrane enclosing a narrow intra-thylakoid space (Lackie and Dow, Dictionary of Cell Biology, 2nd ed). Individual thylakoids are interconnected and tend to stack to form aggregates called grana. They are found in cyanobacteria and all plants.
Axoneme
A bundle of MICROTUBULES and MICROTUBULE-ASSOCIATED PROTEINS forming the core of each CILIUM or FLAGELLUM. In most eukaryotic cilia or flagella, an axoneme shaft has 20 microtubules arranged in nine doublets and two singlets.
Light-Harvesting Protein Complexes
Complexes containing CHLOROPHYLL and other photosensitive molecules. They serve to capture energy in the form of PHOTONS and are generally found as components of the PHOTOSYSTEM I PROTEIN COMPLEX or the PHOTOSYSTEM II PROTEIN COMPLEX.
Protozoan Proteins
Proteins found in any species of protozoan.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Mutation
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Arylsulfatases
Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.
Diuron
A pre-emergent herbicide.
Dyneins
A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.
Transformation, Genetic
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Amylopectin
A highly branched glucan in starch.
Plastoquinone
Polyunsaturated side-chain quinone derivative which is an important link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
Volvox
A genus of GREEN ALGAE in the family Volvocaceae. They form spherical colonies of hundreds or thousands of bi-flagellated cells in a semi-transparent gelatinous ball.
Ribulose-Bisphosphate Carboxylase
A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.
Darkness
The absence of light.
Sulfur
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
Cytochromes c6
Cytochromes of the c type that are involved in the transfer of electrons from CYTOCHROME B6F COMPLEX and PHOTOSYSTEM I.
Microalgae
A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.
RNA, Chloroplast
Ribonucleic acid in chloroplasts having regulatory and catalytic roles as well as involvement in protein synthesis.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Chloroplast Proteins
Proteins encoded by the CHLOROPLAST GENOME or proteins encoded by the nuclear genome that are imported to and resident in the CHOROPLASTS.
DNA, Chloroplast
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Hydrogenase
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
Carbonic Anhydrases
A family of zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide. They play an important role in the transport of CARBON DIOXIDE from the tissues to the LUNG. EC 4.2.1.1.
Cytochrome b Group
Cytochromes (electron-transporting proteins) with protoheme (HEME B) as the prosthetic group.
Electron Transport
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Malate Dehydrogenase (NADP+)
Genetic Complementation Test
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Carbon Dioxide
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
Starch
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
Hydrogen
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Cytochromes
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
Genes, Plant
The functional hereditary units of PLANTS.
Chlorella
Nonmotile unicellular green algae potentially valuable as a source of high-grade protein and B-complex vitamins.
Starch Synthase
An enzyme that catalyzes the transfer of glucose from ADPglucose to glucose-containing polysaccharides in 1,4-alpha-linkages. EC 2.4.1.21.
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
An enzyme that catalyzes the condensation of two molecules of geranylgeranyl diphosphate to give prephytoene diphosphate. The prephytoene diphosphate molecule is a precursor for CAROTENOIDS and other tetraterpenes.
Organelle Size
The quantity of volume or surface area of ORGANELLES.
Ferredoxins
Iron-containing proteins that transfer electrons, usually at a low potential, to flavoproteins; the iron is not present as in heme. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
RNA, Protozoan
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Phylogeny
The relationships of groups of organisms as reflected by their genetic makeup.
Kinetics
The rate dynamics in chemical or physical systems.
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Microtubules
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Phototrophic Processes
Processes by which phototrophic organisms use sunlight as their primary energy source. Contrasts with chemotrophic processes which do not depend on light and function in deriving energy from exogenous chemical sources. Photoautotrophy (or photolithotrophy) is the ability to use sunlight as energy to fix inorganic nutrients to be used for other organic requirements. Photoautotrophs include all GREEN PLANTS; GREEN ALGAE; CYANOBACTERIA; and green and PURPLE SULFUR BACTERIA. Photoheterotrophs or photoorganotrophs require a supply of organic nutrients for their organic requirements but use sunlight as their primary energy source; examples include certain PURPLE NONSULFUR BACTERIA. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; HETEROTROPHY; chemotrophy; or phototrophy) to utilize different sources to meet their nutrients and energy requirements.
Isoamylase
An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 3.2.1.41) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 3.2.1.69) by its action on glycogen. With EC 3.2.1.69, it produces the activity called "debranching enzyme". EC 3.2.1.68.
Pyruvate Synthase
A ferredoxin-containing enzyme that catalyzes the COENZYME A-dependent oxidative decarboxylation of PYRUVATE to acetyl-COENZYME A and CARBON DIOXIDE.
Gene Expression Regulation, Plant
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Electrophoresis, Polyacrylamide Gel
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Phenotype
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Fluorescence
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Oxygen
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Thioredoxin h
A thioredoxin subtype that is ubiquitously found in the plant kingdom. It reduces a variety of seed storage proteins and may play a role in the germination process of seeds.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Coproporphyrinogen Oxidase
An enzyme that catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX by the conversion of two propionate groups to two vinyl groups. It is the sixth enzyme in the 8-enzyme biosynthetic pathway of HEME, and is encoded by CPO gene. Mutations of CPO gene result in HEREDITARY COPROPORPHYRIA.
Cilia
Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)
DNA, Protozoan
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Eukaryota
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
Genome, Chloroplast
The genetic complement of CHLOROPLASTS as represented in their DNA.
Molecular Weight
The sum of the weight of all the atoms in a molecule.
Iron-Sulfur Proteins
A group of proteins possessing only the iron-sulfur complex as the prosthetic group. These proteins participate in all major pathways of electron transport: photosynthesis, respiration, hydroxylation and bacterial hydrogen and nitrogen fixation.
Anaerobiosis
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
DNA, Complementary
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Genome, Protozoan
The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.
Centrioles
Self-replicating, short, fibrous, rod-shaped organelles. Each centriole is a short cylinder containing nine pairs of peripheral microtubules, arranged so as to form the wall of the cylinder.
Tubulin
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
beta-Tocopherol
A natural tocopherol with less antioxidant activity than alpha-tocopherol. It exhibits antioxidant activity by virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus. As in GAMMA-TOCOPHEROL, it also has three methyl groups on the 6-chromanol nucleus but at different sites.
Spectinomycin
An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of gonorrhea.
Crosses, Genetic
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.
Plastids
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
RNA, Plant
Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis.
Photophosphorylation
The use of light to convert ADP to ATP without the concomitant reduction of dioxygen to water as occurs during OXIDATIVE PHOSPHORYLATION in MITOCHONDRIA.
Carbon
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.

Role of a novel photosystem II-associated carbonic anhydrase in photosynthetic carbon assimilation in Chlamydomonas reinhardtii. (1/1304)

Intracellular carbonic anhydrases (CA) in aquatic photosynthetic organisms are involved in the CO2-concentrating mechanism (CCM), which helps to overcome CO2 limitation in the environment. In the green alga Chlamydomonas reinhardtii, this CCM is initiated and maintained by the pH gradient created across the chloroplast thylakoid membranes by photosystem (PS) II-mediated electron transport. We show here that photosynthesis is stimulated by a novel, intracellular alpha-CA bound to the chloroplast thylakoids. It is associated with PSII on the lumenal side of the thylakoid membranes. We demonstrate that PSII in association with this lumenal CA operates to provide an ample flux of CO2 for carboxylation.  (+info)

Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that encode novel proteins containing ankyrin repeats and WW domains. (2/1304)

Genes that are expressed only in the young zygote are considered to be of great importance in the development of an isogamous green alga, Chlamydomonas reinhardtii. Clones representing the Zys3 gene were isolated from a cDNA library prepared using zygotes at 10 min after fertilization. Sequencing of Zys3 cDNA clones resulted in the isolation of two related molecular species. One of them encoded a protein that contained two kinds of protein-to-protein interaction motifs known as ankyrin repeats and WW domains. The other clone lacked the ankyrin repeats but was otherwise identical. These mRNA species began to accumulate simultaneously in cells beginning 10 min after fertilization, and reached maximum levels at about 4 h, after which time levels decreased markedly. Genomic DNA gel-blot analysis indicated that Zys3 was a single-copy gene. The Zys3 proteins exhibited parallel expression to the Zys3 mRNAs at first, appearing 2 h after mating, and reached maximum levels at more than 6 h, but persisted to at least 1 d. Immunocytochemical analysis revealed their localization in the endoplasmic reticulum, which suggests a role in the morphological changes of the endoplasmic reticulum or in the synthesis and transport of proteins to the Golgi apparatus or related vesicles.  (+info)

Photosystem I is indispensable for photoautotrophic growth, CO2 fixation, and H2 photoproduction in Chlamydomonas reinhardtii. (3/1304)

Certain Chlamydomonas reinhardtii mutants deficient in photosystem I due to defects in psaA mRNA maturation have been reported to be capable of CO2 fixation, H2 photoevolution, and photoautotrophic growth (Greenbaum, E., Lee, J. W., Tevault, C. V., Blankinship, S. L. , and Mets, L. J. (1995) Nature 376, 438-441 and Lee, J. W., Tevault, C. V., Owens, T. G.; Greenbaum, E. (1996) Science 273, 364-367). We have generated deletions of photosystem I core subunits in both wild type and these mutant strains and have analyzed their abilities to grow photoautotrophically, to fix CO2, and to photoevolve O2 or H2 (using mass spectrometry) as well as their photosystem I content (using immunological and spectroscopic analyses). We find no instance of a strain that can perform photosynthesis in the absence of photosystem I. The F8 strain harbored a small amount of photosystem I, and it could fix CO2 and grow slowly, but it lost these abilities after deletion of either psaA or psaC; these activities could be restored to the F8-psaADelta mutant by reintroduction of psaA. We observed limited O2 photoevolution in mutants lacking photosystem I; use of 18O2 indicated that this O2 evolution is coupled to O2 uptake (i.e. respiration) rather than CO2 fixation or H2 evolution. We conclude that the reported instances of CO2 fixation, H2 photoevolution, and photoautotrophic growth of photosystem I-deficient mutants result from the presence of unrecognized photosystem I.  (+info)

Induction of coproporphyrinogen oxidase in Chlamydomonas chloroplasts occurs via transcriptional regulation of Cpx1 mediated by copper response elements and increased translation from a copper deficiency-specific form of the transcript. (4/1304)

Coproporphyrinogen III oxidase, encoded by a single nuclear gene in Chlamydomonas reinhardtii, produces three distinct transcripts. One of these transcripts is greatly induced in copper-deficient cells by transcriptional activation, whereas the other forms are copper-insensitive. The induced form of the transcript was expressed coordinately with the cytochrome c6-encoding (Cyc6) gene, which is known to be transcriptionally regulated in copper-deficient cells. The sequence GTAC, which forms the core of a copper response element associated with the Cyc6 gene, is also essential for induction of the Cpx1 gene, suggesting that both are targets of the same signal transduction pathway. The constitutive and induced Cpx1 transcripts have the same half-lives in vivo, and all encode the same polypeptide with a chloroplast-targeting transit sequence, but the shortest one representing the induced form is a 2-4-fold better template for translation than are either of the constitutive forms. The enzyme remains localized to a soluble compartment in the chloroplast even in induced cells, and its abundance is not affected when the tetrapyrrole pathway is manipulated either genetically or by gabaculine treatment.  (+info)

Group II intron splicing in Escherichia coli: phenotypes of cis-acting mutations resemble splicing defects observed in organelle RNA processing. (5/1304)

The mitochondrial group IIB intron rI1, from the green algae Scenedesmus obliquus ' LSUrRNA gene, has been introduced into the lacZ gene encoding beta-galacto-sidase. After DNA-mediated transformation of the recombinant lacZ gene into Escherichia coli, we observed correct splicing of the chimeric precursor RNA in vivo. In contrast to autocatalytic in vitro self-splicing, intron processing in vivo is independent of the growth temperature, suggesting that in E.coli, trans -acting factors are involved in group II intron splicing. Such a system would seem suitable as a model for analyzing intron processing in a prokaryotic host. In order to study further the effect of cis -mutations on intron splicing, different rI1 mutants were analyzed (with respect to their splicing activity) in E.coli. Although the phenotypes of these E. coli intron splicing mutants were identical to those which can be observed during organellar splicing of rI1, they are different to those observed in in vitro self-splicing experiments. Therefore, in both organelles and prokaryotes, it is likely that either similar splicing factors or trans -acting factors exhibiting similar functions are involved in splicing. We speculate that ubiquitous trans -acting factors, via recent horizontal transfer, have contributed to the spread of group II introns.  (+info)

Group II intron splicing in chloroplasts: identificationof mutations determining intron stability and fate of exon RNA. (6/1304)

In order to investigate in vivo splicing of group II introns in chloroplasts, we previously have integrated the mitochondrial intron rI1 from the green alga Scenedesmus obliquus into the Chlamydomonas chloroplast tscA gene. This construct allows a functional analysis of conserved intron sequences in vivo, since intron rI1 is correctly spliced in chloroplasts. Using site-directed mutagenesis, deletions of the conserved intron domains V and VI were performed. In another set of experiments, each possible substitution of the strictly conserved first intron nucleotide G1 was generated, as well as each possible single and double mutation of the tertiary base pairing gamma-gamma ' involved in the formation of the intron's tertiary RNA structure. In most cases, the intron mutations showed the same effect on in vivo intron splicing efficiency as they did on the in vitro self-splicing reaction, since catalytic activity is provided by the intron RNA itself. In vivo, all mutations have additional effects on the chimeric tscA -rI1 RNA, most probably due to the role played by trans -acting factors in intron processing. Substitutions of the gamma-gamma ' base pair lead to an accumulation of excised intron RNA, since intron stability is increased. In sharp contrast to autocatalytic splicing, all point mutations result in a complete loss of exon RNA, although the spliced intron accumulates to high levels. Intron degradation and exon ligation only occur in double mutants with restored base pairing between the gamma and gamma' sites. Therefore, we conclude that intron degradation, as well as the ligation of exon-exon molecules, depends on the tertiary intron structure. Furthermore, our data suggest that intron excision proceeds in vivo independent of ligation of exon-exon molecules.  (+info)

Identification of cis-acting RNA leader elements required for chloroplast psbD gene expression in Chlamydomonas. (7/1304)

The psbD mRNA of Chlamydomonas reinhardtii is one of the most abundant chloroplast transcripts and encodes the photosystem II reaction center polypeptide D2. This RNA exists in two forms with 5' untranslated regions of 74 and 47 nucleotides. The shorter form, which is associated with polysomes, is likely to result from processing of the larger RNA. Using site-directed mutagenesis and biolistic transformation, we have identified two major RNA stability determinants within the first 12 nucleotides at the 5' end and near position -30 relative to the AUG initiation codon of psbD. Insertion of a polyguanosine tract at position -60 did not appreciably interfere with translation of psbD mRNA. The same poly(G) insertion in the nac2-26 mutant, which is known to be deficient in psbD mRNA accumulation, stabilized the psbD RNA. However, the shorter psbD RNA did not accumulate, and the other psbD RNAs were not translated. Two other elements were found to affect translation but not RNA stability. The first comprises a highly U-rich sequence (positions -20 to -15), and the second, called PRB1 (positions -14 to -11), is complementary to the 3' end of the 16S rRNA. Changing the PRB1 sequence from GGAG to AAAG had no detectable effect on psbD mRNA translation. However, changing this sequence to CCUC led to a fourfold diminished rate of D2 synthesis and accumulation. When the psbD initiation codon was changed to AUA or AUU, D2 synthesis was no longer detected, and psbD RNA accumulated to wild-type levels. The singular organization of the psbD 5' untranslated region could play an important role in the control of initiation of psbD mRNA translation.  (+info)

Direct measurement of inter-doublet elasticity in flagellar axonemes. (8/1304)

The outer doublet microtubules in ciliary and flagellar axonemes are presumed to be connected with each other by elastic links called the inter-doublet links or the nexin links, but it is not known whether there actually are such elastic links. In this study, to detect the elasticity of the putative inter-doublet links, shear force was applied to Chlamydomonas axonemes with a fine glass needle and the longitudinal elasticity was determined from the deflection of the needle. Wild-type axonemes underwent a high-frequency, nanometer-scale vibration in the presence of ATP. When longitudinal shear force was applied, the average position of the needle tip attached to the axoneme moved linearly with the force applied, yielding an estimate of spring constant of 2.0 (S.D.: 0.8) pN/nm for 1 microm of axoneme. This value did not change in the presence of vanadate, i.e., when dynein does not form strong cross bridges. In contrast, it was at least five times larger when ATP was absent, i.e., when dynein forms strong cross bridges. The measured elasticity did not significantly differ in various mutant axonemes lacking the central-pair microtubules, a subset of inner-arm dynein, outer-arm dynein, or the radial spokes, although it was somewhat smaller in the latter two mutants. It was also observed that the shear displacement in an axoneme in the presence of ATP often took place in a stepwise manner. This suggests that the inter-doublet links can reversibly detach from and reattach to the outer doublets in a cooperative manner. This study thus provides the first direct measure of the elasticity of inter-doublet links and also demonstrates its dynamic nature.  (+info)

Biomolecules | Free Full-Text | The TOR Signaling Network in the Model Unicellular Green Alga Chlamydomonas reinhardtii | HTMLBiomolecules | Free Full-Text | The TOR Signaling Network in the Model Unicellular Green Alga Chlamydomonas reinhardtii | HTML
Cell growth is tightly coupled to nutrient availability. The target of rapamycin (TOR) kinase transmits nutritional and environmental cues to the cellular growth machinery. TOR functions in two distinct multiprotein complexes, termed TOR complex 1 (TORC1) and TOR complex 2 (TORC2). While the structure and functions of TORC1 are highly conserved in all eukaryotes, including algae and plants, TORC2 core proteins seem to be missing in photosynthetic organisms. TORC1 controls cell growth by promoting anabolic processes, including protein synthesis and ribosome biogenesis, and inhibiting catabolic processes such as autophagy. Recent studies identified rapamycin-sensitive TORC1 signaling regulating cell growth, autophagy, lipid metabolism, and central metabolic pathways in the model unicellular green alga Chlamydomonas reinhardtii. The central role that microalgae play in global biomass production, together with the high biotechnological potential of these organisms in biofuel production, has drawn attention
http://www.mdpi.com/2218-273X/7/3/54/htm
Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii, Plant Molecular Biology |...Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii, Plant Molecular Biology |...
Read Characterization of the LI818 polypeptide from the green unicellular alga Chlamydomonas reinhardtii, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/characterization-of-the-li818-polypeptide-from-the-green-unicellular-MRFa7lmeVp
Expression of a histidine-tagged RNA pyrophosphohydrolase in Chlamydomonas reinhardtii for localization studies
RNA pyrophosphohydrolase (RppH) catalyzes the removal of pyrophosphate from 5 triphosphorylated RNAs thereby initiating RNA degradation. The enzyme has originally been identified in bacteria but homologs are present in eukaryotes where they are thought to be located in plastids or mitochondria. A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5rppH-GFP construct.. A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, sequencing, and DNA and RNA blotting techniques were used to indentify positive transformants at the DNA ...
https://www.duo.uio.no/handle/10852/36323
Frontiers | Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii |...Frontiers | Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii |...
The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. Using a forward genetics approach, we have identified and characterized a mutant x32, which lacks a predicted protein named CGLD1 (Conserved in Green Lineage and Diatom 1) in GreenCut2, under normal and stress conditions. We show that loss of CGLD1 resulted in minimal photoautotrophic growth and PSII activity in the organism. We observed reduced amount of PSII complex and core subunits in the x32 mutant based on blue-native (BN)/PAGE and immunoblot analysis. Moreover, x32 exhibited increased sensitivity to high-light stress and altered tolerance to different reactive oxygenic species (ROS) stress treatments, i.e. decreased resistance to H2O2/or tert-Butyl hydroperoxide (t-BOOH) and increased tolerance to neutral red (NR) and rose bengal (RB) that induce the formation of singlet oxygen, respectively. Further analysis via quantitative real-time PCR (qRT-PCR)
https://www.frontiersin.org/articles/10.3389/fpls.2017.02154/full
Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase | ScienceTransgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase | Science
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families.Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes. ...
http://science.sciencemag.org/content/290/5494/1159
3′-Processed mRNA Is Preferentially Translated in Chlamydomonas reinhardtii Chloroplasts | Molecular and Cellular Biology3′-Processed mRNA Is Preferentially Translated in Chlamydomonas reinhardtii Chloroplasts | Molecular and Cellular Biology
The data presented here suggest that 3′ end processing may be required for translation of atpB and rbcLmRNAs in Chlamydomonas chloroplasts. Unprocessed atpB transcripts, defined as those that do not accumulate as an abundant size class of approximately 2 kb, were only present in nonpolysomal fractions. Processed mRNAs were present in both polysomal and nonpolysomal fractions. Since the 3′ ends of most chloroplast transcripts are generated from longer pre-mRNAs by exo- and/or endonucleolytic mechanisms (17, 36, 37, 44, 47), this 3′ processing apparatus may interact with or signal the translational machinery.. Our ability to detect a heterogeneous collection of putative processing intermediates or incorrectly processed transcripts for atpBand rbcL suggests that these molecules are relatively stable in the chloroplast. When they were analyzed by RNase protection, it was possible to detect partially processed transcripts in theChlamydomonas chloroplast petD-trnR region (29), and in certain ...
https://mcb.asm.org/content/18/8/4605
Reentrant Efficiency of Phototaxis in Chlamydomonas reinhardtii Cells ePrints@IIScReentrant Efficiency of Phototaxis in Chlamydomonas reinhardtii Cells [email protected]
Phototaxis is one of the most fundamental stimulus-response behaviors in biology wherein motile microorganisms sense light gradients to swim toward the light source. Apart from single-cell survival and growth, it plays a major role at the global scale of aquatic ecosystems and bioreactors. We study phototaxis of single-celled algae Chlamydomonas reinhardtii as a function of cell number density and light stimulus using high spatiotemporal video microscopy. Surprisingly, the phototactic efficiency has a minimum at a well-defined number density, for a given light gradient, above which the phototaxis behavior of a collection of cells can even exceed the performance obtainable from single isolated cells. We show that the origin of enhancement of performance above the critical concentration lies in the slowing down of the cells, which enables them to sense light more effectively. We also show that this steady-state phenomenology is well captured by modeling the phototactic response as a ...
http://eprints.iisc.ac.in/63824/
The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii<...
TY - JOUR. T1 - The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii. AU - Ozawa, Shin Ichiro. AU - Cavaiuolo, Marina. AU - Jarrige, Domitille. AU - Kuras, Richard. AU - Rutgers, Mark. AU - Eberhard, Stephan. AU - Drapier, Dominique. AU - Wollman, Francis André. AU - Choquet, Yves. PY - 2020/4/1. Y1 - 2020/4/1. N2 - In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. Here, we report the identification of the octotricopeptide repeat protein MTHI1, which is critical for the biogenesis of chloroplast ATP synthase oligomycin-sensitive chloroplast coupling factor. Unlike most trans-acting factors characterized so far in Chlamydomonas, which control the expression of a single gene, MTHI1 targets two distinct transcripts: it is required for the accumulation and translation of atpH mRNA, encoding a subunit of ...
https://okayama.pure.elsevier.com/en/publications/the-opr-protein-mthi1-controls-the-expression-of-two-different-su
Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis by Kristy Marie BrumfieldInvestigations of Chlamydomonas reinhardtii ergosterol biosynthesis by Kristy Marie Brumfield
Ergosterol is the major sterol found in the membranes of Chlamydomonas reinhardtii. While past studies have identified some ergosterol mutants in C. reinhardtii, very little is known about sterol biosynthesis pathways in this species. With the elucidation of the Chlamydomonas genome, bioinformatics analysis has allowed us to determine potential genes involved in ergosterol biosynthesis. With this knowledge, a working model of the pathway was designed for future analysis. Several of the ergosterol biosynthetic genes were analyzed in respect to their role and involvement in flagellar regeneration. These genes were upregulated during the regrowth of the flagella. Also Chlamydomonas strains lacking flagella were analyzed by Q-RT PCR to determine what role ergosterol biosynthetic genes played in the absence of their flagella. Finally, one of the genes with homology to the yeast sterol C-5 desaturase, ERG3, was chosen for further analysis. To test whether ERG3 of C. reinhardtii had a similar function, yeast
https://digitalcommons.lsu.edu/gradschool_dissertations/2296/
Flagellar Synchronization Is a Simple Alternative to Cell Cycle Synchronization for Ciliary and Flagellar StudiesFlagellar Synchronization Is a Simple Alternative to Cell Cycle Synchronization for Ciliary and Flagellar Studies
The unicellular green alga Chlamydomonas reinhardtii is an ideal model organism for studies of ciliary function and assembly. In assays for biological and biochemical effects of various factors on flagellar structure and function, synchronous culture is advantageous for minimizing variability. Here, we have characterized a method in which 100% synchronization is achieved with respect to flagellar length but not with respect to the cell cycle. The method requires inducing flagellar regeneration by amputation of the entire cell population and limiting regeneration time. This results in a maximally homogeneous distribution of flagellar lengths at 3 h postamputation. We found that time-limiting new protein synthesis during flagellar synchronization limits variability in the unassembled pool of limiting flagellar protein and variability in flagellar length without affecting the range of cell volumes. We also found that long- and short-flagella mutants that regenerate normally require longer and ...
https://kuscholarworks.ku.edu/handle/1808/24889
Latest - UCLA-DOE Institute
The unicellular green alga Chlamydomonas reinhardtii excels at acclimating to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day to coordinate cellular growth before undergoing cell division. Parallel measurements of select metabolites and pigments, physiological parameters and a subset of proteins allow us to infer metabolic events and to evaluate the impact of the transcriptome on the proteome. Among new findings is the observation that Chlamydomonas exhibits low respiratory activity at night and relies instead on fermentative metabolism; we propose that the ferredoxin FDX9 acts as the electron donor to fermentative hydrogenases. The light stress responsive genes PSBS, LHCSR1 and LHCSR3 all show an acute response to light at dawn under abrupt dark-to light transitions. LHCSR3 genes also ...
https://www.doe-mbi.ucla.edu/latest/
中国科学院水生生物研究所机构知识库(IHB OpenIR): Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis ...
http://ir.ihb.ac.cn/handle/152342/9402
Adenosine 3:5-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization. | Semantic ScholarAdenosine 3:5-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization. | Semantic Scholar
Chlamydomonas reinhardtii contains a factor that can replace adenosine 3:5-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. The factor cochromatographs and coelectrophoreses with authentic cAMP, and is inactivated by beef heart cyclic nucleotide phosphodiesterase. When C. reinhardtii is exposed to aminophylline (theophylline(2) ethylenediamine), the concentration of the factor in the cells increases within 1 hr, from about 25 pmol of cAMP equivalents per g dry weight to more than 250 pmol. Cyclic nucleotide phosphodiesterase activity is present in crude extract of C. reinhardtii and is inhibited by theophylline. We conclude that cAMP occurs in C. reinhardtii and that the endogenous concentration is governed at least in part by a theophylline-sensitive cyclic nucleotide phosphodiesterase. These findings provide a sound basis for attributing the effects of methylxanthines on flagellar function and regeneration in C. reinhardtii to the resultant elevation of endogenous cAMP
https://www.semanticscholar.org/paper/Adenosine-3%27%3A5%27-Cyclic-Monophosphate-in-Isolation-Amrhein-Filner/c7c28c11525314cb90a6ed2949870bf09bca8444
Assembly-controlled regulation of chloroplast gene translationAssembly-controlled regulation of chloroplast gene translation
Studies of the biogenesis of the photosynthetic protein complexes in the unicellular green alga Chlamydomonas reinhardtii have pointed to the importance of the concerted expression of nuclear and chloroplast genomes. The accumulation of chloroplast- and nuclear-encoded subunits is concerted, most of …
https://pubmed.ncbi.nlm.nih.gov/11498001/?dopt=Abstract
Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii -...Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii -...
The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. Current models of the CCM postulate that aseries of ion transporters bring HCO3- from outside the cell to thethylakoid lumen, where the carbonic anhydrase CAH3 dehydratesaccumulated HCO3- to CO2, raising the CO2 concentration forRubisco. Previously, HCO3- transporters have been identified atboth the plasma membrane and the chloroplast envelope, butthe transporter thought to be on the thylakoid membrane hasnot been identified. Three paralogous genes (BST1, BST2, BST3)belonging to the bestrophin family have been found to be upregulatedin low CO2 conditions, and their expression is controlledby CIA5, a transcription factor that controls many CCM genes.YFP fusions demonstrate that all three proteins are located onthe thylakoid membrane, and interactome studies indicate thatthey might associate with chloroplast CCM components. A singlemutant defective in ...
http://eprints.whiterose.ac.uk/148641/
Characterization of THB1, a Chlamydomonas reinhardtii truncated hemogl by Eric A. Johnson, Selena L. Rice et al.Characterization of THB1, a Chlamydomonas reinhardtii truncated hemogl by Eric A. Johnson, Selena L. Rice et al.
The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added ligand, THB1 coordinates the heme iron with the canonical proximal histidine and a distal lysine. In the cyanomet state, THB1 is structurally similar to other known truncated hemoglobins, particularly the heme domain of Chlamydomonas eugametos LI637, a light-induced chloroplastic hemoglobin. Recombinant THB1 is capable of binding nitric oxide (NO(*)) in either the ferric or ferrous state and has efficient NO(*) dioxygenase activity. By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is
https://escholarship.umassmed.edu/cellbiology_pp/148/
Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii...Crystal structure of activated ribulose-1,5-bisphosphate carboxylase/oxygenase from green alga Chlamydomonas reinhardtii...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/11866526?dopt=Abstract
The Involvement of a Multicopper Oxidase in Iron Uptake by the Green Algae Chlamydomonas reinhardtii | Plant PhysiologyThe Involvement of a Multicopper Oxidase in Iron Uptake by the Green Algae Chlamydomonas reinhardtii | Plant Physiology
In this report, we present novel evidence linking FLP to high-affinity Fe transport and propose that FLP is a ferroxidase, functioning in the reoxidation of Fe2+ before its uptake into the cell. Initial evidence in support of this hypothesis comes from the deduced FLP amino acid sequence. FLP contains two multicopper oxidase I and one multicopper oxidase signature II motifs. In addition, the amino acid sequence of FLP shows the highest homology to multicopper oxidases in mammals (HEPH and ceruloplasmin) and yeast (FET3). These proteins are ferroxidases that are themselves involved in high-affinity Fe assimilation (Stearman et al., 1996;Askwith and Kaplan, 1998; Mukhopadhyay et al., 1998; Attieh et al., 1999; Vulpe et al., 1999). The involvement of FLP in Fe homeostasis is evident from the regulation of its synthesis. Both the transcription of the FLP gene and synthesis of FLP are greatly increased in Fe-deficient cells and reversed after resupply of Fe. Although we have not demonstrated the ...
http://www.plantphysiol.org/content/130/4/2039?ijkey=67f1c70e56669c4b7a6b7d50480c3e108fe41f9e&keytype2=tf_ipsecsha
Translational regulation of light-harvesting complex expression during photoacclimation to high-light in Chlamydomonas...
TY - JOUR. T1 - Translational regulation of light-harvesting complex expression during photoacclimation to high-light in Chlamydomonas reinhardtii. AU - McKim, S M. AU - Durnford, D G. PY - 2006/11/14. Y1 - 2006/11/14. N2 - When challenged with excess light, the green alga Chlamydomonas reinhardtii responds, in part, by down-regulating light harvesting capacity at Photosystem II while concomitantly reorganising cellular metabolism to increase sink capacity. We examined the role of translational control during different stages of photoacclimation by analysing polysome profiles of two different light-harvesting complex (LHC) genes encoding a major LHCII component (Lhcbm) and CP29 (Lhcb4) plus iron superoxide dismutase (FeSOD), and through measurement of protein synthesis by in vivo labelling. Within two hours following transfer of low-light (LL) acclimated cultures into high-light (HL), Lhcbm transcripts are off-loaded from polysomes indicating a decline in translational initiation. Lhcbm ...
https://discovery.dundee.ac.uk/en/publications/translational-regulation-of-light-harvesting-complex-expression-d
Ultrastructural and biochemical analysis of a new mutation in Chlamydomonas reinhardtii affecting the central pair apparatus -...Ultrastructural and biochemical analysis of a new mutation in Chlamydomonas reinhardtii affecting the central pair apparatus -...
We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.. ...
https://eprints.utas.edu.au/4364/
GFP-Markierung von Cytoskelettproteinen in Chlamydomonas reinhardtii (Chlorophyceae) - Kölner UniversitätsPublikationsServerGFP-Markierung von Cytoskelettproteinen in Chlamydomonas reinhardtii (Chlorophyceae) - Kölner UniversitätsPublikationsServer
Fusion of green fluorescent protein (GFP) to proteins is a powerful method to investigate dynamic processes in vivo. The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP-tagging of proteins was employed in order to analyse proteins of the flagellar basal apparatus. Striated fiber assembling (SFA), centrin and deflagellation induced protein of 13 kDa (DIP13) were tagged with GFP at the C-terminal domain. In addition SFA was tagged at the N-terminal domain. The chimeric genes were stably transformed in C. reinhardtii. SFA is the mayor component of the striated microtubule associated fibers (SMAFS). GFP tagged SFA was incorporated into this fibers. N-terminal tagged SFA had similar properties like the wild-type protein. The length of the fibers increased with the strength of expression. The head domain of SFA is essential for fiber formation and photobleaching experiments did not show a pronounced dynamic of the fibers. The ...
http://kups.ub.uni-koeln.de/1251/
Carbonic Anhydrase and Carbonic Anhydrase like Genes of Chlamydomonas Reinhardtii - OpenThesis
Carbonic anhydrase (CA) is a zinc containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3-. There are three evolutionarily unrelated CA families designated alpha, beta and gamma CA. Vertebrates have members of the alpha CA family, while higher plants, algae and cyanobacteria have members belonging to all three CA families. In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta CAs. This dissertation describes the identification and characterization of new CA genes from C. reinhardtii. Four new CA or CA like genes have been discovered including two beta CAs and two gamma CAs. Three CAs were investigated further including the alpha CA Cah3, one of the new beta CAs, Cah6; and a new gamma CA designated Gclp1 for gamma CA like protein. Cah3 is an alpha CA located in the thylakoid. Past studies with two Cah3 mutants, ca-1 and cia3 have shown that Cah3 plays an important role in the CO2 concentrating ...
http://www.openthesis.org/documents/Carbonic-Anhydrase-like-Genes-Chlamydomonas-525792.html
Assembly and motility of eukaryotic cilia and flagella. Lessons from Chlamydomonas reinhardtii<...Assembly and motility of eukaryotic cilia and flagella. Lessons from Chlamydomonas reinhardtii<...
TY - JOUR. T1 - Assembly and motility of eukaryotic cilia and flagella. Lessons from Chlamydomonas reinhardtii. AU - Silflow, Carolyn D.. AU - Lefebvre, Paul A.. PY - 2001. Y1 - 2001. UR - http://www.scopus.com/inward/record.url?scp=85047681474&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85047681474&partnerID=8YFLogxK. U2 - 10.1104/pp.010807. DO - 10.1104/pp.010807. M3 - Review article. C2 - 11743094. AN - SCOPUS:85047681474. VL - 127. SP - 1500. EP - 1507. JO - Plant Physiology. JF - Plant Physiology. SN - 0032-0889. IS - 4. ER - ...
https://experts.umn.edu/en/publications/assembly-and-motility-of-eukaryotic-cilia-and-flagella-lessons-fr-2
Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration - Cardol...Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration - Cardol...
en] Photosynthetic activities were analyzed in Chlamydomonas reinhardtii mitochondrial mutants affected in different complexes (I, III, IV, I + III, and I + IV) of the respiratory chain. Oxygen evolution curves showed a positive relationship between the apparent yield of photosynthetic linear electron transport and the number of active proton-pumping sites in mitochondria. Although no significant alterations of the quantitative relationships between major photosynthetic complexes were found in the mutants, 77 K fluorescence spectra showed a preferential excitation of photosystem I (PSI) compared with wild type, which was indicative of a shift toward state 2. This effect was correlated with high levels of phosphorylation of light-harvesting complex II polypeptides, indicating the preferential association of light-harvesting complex II with PSI. The transition to state 1 occurred in untreated wild-type cells exposed to PSI light or in 3-(3,4-dichlorophenyl)-1,1-dimethylurea-treated cells exposed ...
http://orbi.ulg.ac.be/handle/2268/35194
The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells. | Journal of Cell...The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells. | Journal of Cell...
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells ...
https://rupress.org/jcb/article/107/2/635/28533/The-centrin-based-cytoskeleton-of-Chlamydomonas
Results for cd08529Results for cd08529
Catalytic domain of the Protein Serine/Threonine Kinase, Chlamydomonas reinhardtii FA2 and similar domains. Serine/Threonine Kinases (STKs), Chlamydomonas reinhardtii FA2-like subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Chlamydomonas reinhardtii FA2-like subfamily belongs to the (NIMA)-related kinase (Nek) family. The Nek family includes seven different Chlamydomonas Neks (CNKs 1-6 and Fa2). This subfamily includes FA2 and CNK4. The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Chlamydomonas reinhardtii FA2 was discovered in a genetic screen for deflagellation-defective mutants. It is essential for basal-body/centriole-associated microtubule severing, and plays a role in cell cycle progression. No cellular ...
http://bioinf.umbc.edu/dmdm/generatelogo.php?domain=STKc_FA2-like&accession=cd08529&prot=22035598
Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella | JCBFunction and dynamics of PKD2 in Chlamydomonas reinhardtii flagella | JCB
To make the CrPKD2-GFP fusion, the C. reinhardtii bacterial artificial chromosome clone 18K16 (https://www.genome.clemson.edu/cgi-bin/orders) was cut with EcoRV and SpeI, generating an ∼18-kb fragment, which included the entire CrPKD2 gene and its promoter. This fragment was subcloned into the EcoRV and SpeI sites of pBluescript II KS+ (Stratagene), generating a plasmid named pHK25. This plasmid was cut with HindIII, generating three fragments. One of these fragments, an 8-kb fragment containing the promoter and most of the genomic DNA encoding CrPKD2, was cloned into the HindIII site of the pBluescript KS+, generating pHK28. A second fragment, a 5.8-kb fragment containing the last two introns, exons, the 3′ UTR of CrPKD2, and the KS+ vector, was religated to produce pHK26.. To tag the CrPKD2 gene, we cloned the GFP gene into a unique EcoRI site in intron 11, flanked by the first intron of RBCS2 (Goldschmidt-Clermont and Rahire, 1986). For this, the two ends of the intron were subcloned as ...
http://jcb.rupress.org/content/179/3/501
Tryptophan analog resistance mutations in Chlamydomonas reinhardtii. | GeneticsTryptophan analog resistance mutations in Chlamydomonas reinhardtii. | Genetics
Forty single gene mutations in Chlamydomonas reinhardtii were isolated based on resistance to the compound 5-methyl anthranilic acid (5-MAA). In other organisms, 5-MAA is converted to 5-methyltryptophan (5-MT) and 5-MT is a potent inhibitor of anthranilate synthase, which catalyzes the first committed step in tryptophan biosynthesis. The mutant strains fall into two phenotypic classes based on the rate of cell division in the absence of 5-MAA. Strains with class I mutations divide more slowly than wild-type cells. These 17 mutations map to seven loci, which are designated MAA1 to MAA7. Strains with class II mutations have generation times indistinguishable from wild-type cells, and 7 of these 23 mutations map to loci defined by class I mutations. The remainder of the class II mutations map to 9 other loci, which are designated MAA8-MAA16. The maa5-1 mutant strain excretes high levels of anthranilate and phenylalanine into the medium. In this strain, four enzymatic activities in the tryptophan ...
https://www.genetics.org/content/131/3/593
中国科学院水生生物研究所机构知识库(IHB OpenIR): MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii
MicroRNAs play an important role in abiotic stress responses in higher plants and animals, but their role in stress adaptation in algae remains unknown. In this study, the expression of identified and putative miRNAs in Chlamydomonas reinhardtii was assessed using quantitative polymerase chain reaction; some of the miRNAs (Cre-miR906-3p) were up-regulated, whereas others (Cre-miR910) were downregulated when the species was subjected to multiple abiotic stresses. With degradome sequencing data, we also identified ATP4 (the d-subunit of ATP synthase) and NCR2 (NADPH: cytochrome P450 reductase) as one of the several targets of Cre-miR906-3p and Cre-miR910, respectively. Q-PCR data indicated that ATP4, which was expressed inversely in relation to Cre-miR906-3p under stress conditions. Overexpressing of Cre-miR906-3p enhanced resistance to multiple stresses; conversely, overexpressing of ATP4 produced the opposite effect. These data of Q-PCR, degradome sequencing and adaptation of overexpressing ...
http://ir.ihb.ac.cn/handle/342005/28695?mode=full&submit_simple=Show+full+item+record
Identification of nitrate transporter genes in Chlamydomonas reinhardtiiIdentification of nitrate transporter genes in Chlamydomonas reinhardtii
The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the nitrate reductase structural gene nit-1, have been related to nitrate transport. Mutant strains defective in nitrate transport and having an active nitrate reductase have bee …
https://pubmed.ncbi.nlm.nih.gov/8180624/?dopt=Abstract
The Green Algae Chlamydomonas With Its Two Flagella Sem X6000 Stock Photo | Getty ImagesThe Green Algae Chlamydomonas With Its Two Flagella Sem X6000 Stock Photo | Getty Images
View Stock Photo of The Green Algae Chlamydomonas With Its Two Flagella Sem X6000. Find premium, high-resolution photos at Getty Images.
https://www.gettyimages.com/detail/photo/the-green-algae-chlamydomonas-with-its-two-high-res-stock-photography/vis900189
The Skaggs Institute for Chemical Biology - 2007 Scientific ReportThe Skaggs Institute for Chemical Biology - 2007 Scientific Report
S.P. Mayfield, P. Lee, P. Pettersson, J. Marìn-Navarro, A. Manuell, M. Muto, M. Tran We have developed a system for expressing recombinant proteins, including human therapeutic proteins, in the chloroplast of the eukaryotic green alga Chlamydomonas reinhardtii. Expression of therapeutic proteins in eukaryotic algae offers several advantages over more traditional protein expression systems. Algae are efficient at producing complex mammalian proteins, stable transgenic lines can be generated in a few weeks, and algal systems can be scaled to high levels for a fraction of the cost of traditional fermentation systems.. We have expressed several recombinant proteins in algae, including human monoclonal antibodies. Antibodies are complex multiprotein molecules that are difficult to express in simple expression systems and expensive to produce in mammalian cell culture. We have had good success in producing these complex proteins in algal chloroplasts. We have also expressed eukaryotic protein toxins, ...
http://www.scripps.edu/news/scientificreports/sk2007/sk07mayfield.html
Beyond Complementation. Map-Based Cloning in Chlamydomonas reinhardtii | Plant PhysiologyBeyond Complementation. Map-Based Cloning in Chlamydomonas reinhardtii | Plant Physiology
Our initial mapping data placed Mcd4 into two possible locations. In both cases, it was desirable to generate bacterial artificial chromosome (BAC) contigs for eventual complementation, as well as additional markers. As a case study, we describe how BAC contigs can be extended using Chlamydomonas resources and our experience in generating site-specific markers.. Several BAC libraries have been constructed for Chlamydomonas, two of which are available through the Clemson Genomics Institute (http://www.genome.clemson.edu/groups/bac/). In addition, BAC contigs (http://www.biology.duke.edu/chlamy_genome/BAC/index.html) have been assembled for most of the STS and RFLP markers. In the case of mcd4, Gsp1 resides on scaffold 2 and CNA45 on scaffold 66. A 41-BAC contig exists for scaffold 2 covering approximately 1,000 kb (R. Nguyen, personal communication), and a smaller contig is linked to scaffold 66 and CNA45 (Fig. 6). An unknown amount of DNA separates scaffolds 2 and 66; such discontinuities in the ...
http://www.plantphysiol.org/content/137/2/557?ijkey=5beda14cb3fba9d92bed84da1dbdbd668de7c0f1&keytype2=tf_ipsecsha
Chlamydomonas Are Common Unicellular Green Algae With Two Flagella Sem Foto de stock | Getty ImagesChlamydomonas Are Common Unicellular Green Algae With Two Flagella Sem Foto de stock | Getty Images
Veja Foto de stock de Chlamydomonas Are Common Unicellular Green Algae With Two Flagella Sem. Encontre fotografias premium e de alta resolução na Getty Images.
https://www.gettyimages.pt/detail/foto/chlamydomonas-are-common-unicellular-green-algae-fotografia-de-stock/vis899097
Links - SPLASH - Sustainable PoLymers from Algae Sugars and HydrocarbonsLinks - SPLASH - Sustainable PoLymers from Algae Sugars and Hydrocarbons
The project covers the whole process chain from optimized biomass production to product development and exploitation. In a first step, two industrial bioproduction platforms will be explored: the green alga Botryococcus braunii and the green microalga Chlamydomonas reinhardtii, to which the unique hydrocarbon and polysaccharides producing genes from Botryococcus will be transferred. Biomass cultivation is targeted to reach a pilot scale.
http://eu-splash.eu/links/
Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body...Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body...
Centrin, a 20-kD phosphoprotein with four calcium-binding EF-hands, is present in the centrosome/basal body apparatus of the green alga Chlamydomonas reinhardtii in three distinct locations: the nucleus-basal body connectors, the distal striated fibers, and the flagellar transition regions. In each location, centrin is found in fibrous structures that display calcium-mediated contraction. The mutant vfl2 has structural defects at all of these locations and is defective for basal body localization and/or segregation. We show that the vfl2 mutation is a G-to-A transition in the centrin structural gene which converts a glutamic acid to a lysine at position 101, the first amino acid of the E-helix of the proteins third EF-hand. This proves that centrin is required to construct the nucleus-basal body connectors, the distal striated fibers, and the flagellar transition regions, and it demonstrates the importance of amino acid 101 to normal centrin function. Based on immunofluorescence analysis using ...
https://rupress.org/jcb/article/119/6/1613/56185/Mutational-analysis-of-centrin-an-EF-hand-protein
Princeton researchers explore how a carbon-fixing organelle forms via phase separation - Innovations ReportPrinceton researchers explore how a carbon-fixing organelle forms via phase separation - Innovations Report
While conducting a screen for genes affecting pyrenoid function in the green alga Chlamydomonas reinhardtii, graduate student and the studys first author Alan Itakura and postdoctoral researcher Leif Pallesen, both in the Jonikas group, uncovered a gene called SAGA1 (Starch Granules Abnormal-1), the loss of which causes cells to grow poorly. When the researchers, including the studys co-first author, Kher Xing (Cindy) Chan in Howard Griffiths group at the University of Cambridge, examined the mutant cells, they noticed that SAGA1 mutants possess multiple pyrenoids-up to 10 per cell. This was surprising since normal cells almost always contain just one pyrenoid. Intrigued, the team decided to investigate further.. Because the SAGA1 protein is predicted to contain a starch-binding domain, the researchers first explored whether loss of the SAGA1 gene affects the architecture of the starch plates that make up the pyrenoid sheath. Indeed, the pyrenoids in SAGA1-deficient cells have fewer and ...
https://www.innovations-report.com/life-sciences/princeton-researchers-explore-how-a-carbon-fixing-organelle-forms-via-phase-separation/
Title goes here
High light (HL) stress adversely affects growth, productivity and viability of photosynthetic organisms. The green alga Chlamydomonas reinhardtii is a model system to study photosynthesis and light stress. Comparative proteomics of wild-type and two very high light (VHL)-resistant mutants, VHLR-S4 and VHLR-S9, revealed complex alterations in response to excess light. A twodimensional reference map of the soluble subproteome was constructed representing about 1500 proteins. A total of 83 proteins from various metabolic pathways were identified by peptide mass fingerprinting. Quantitative comparisons of 444 proteins showed 105 significantly changed proteins between wild type and mutants under different light conditions. Commonly, more proteins were decreased than increased, but different proteins were affected in each genotype. Proteins uniquely altered in either VHLR mutant may be involved in VHL resistance. Such candidate proteins similarly altered without light stress, thus possibly ...
http://vlpbp.org/rffbrfirstpaperawardsummarya.html
Molecular Plant Science - LMU MunichMolecular Plant Science - LMU Munich
The goal of our scientific work is the molecular analysis of the biogenesis of photosynthetic thylakoid membranes which represent one of the most complex energy-transducing membranes currently known. We especially ask the questions how the biogenesis process is organized in space, what the molecular working mode of assisting factors is and how these factors developed by evolutionary means.. A second aspect of our work concerns the transformation of a cyanobacterium into an organelle of the plant cell, namely the chloroplast. This domestification of a former free-living procaryote throughout evolution was enabled by the advention of an intracellular communication system harmonizing gene expression in the former cyanobacterium and its host. This system is based on regulatory RNA/protein compexes which we study by applying genetic and biochemical techniques in the unicellular model green alga Chlamydomonas reinhardtii.. ...
http://molplantsci.bio.lmu.de
Health and Healing Archives ⋆ Page 33 of 36 ⋆ LOVE IN ACTION NOWHealth and Healing Archives ⋆ Page 33 of 36 ⋆ LOVE IN ACTION NOW
Editors note: I first read about this in The Celestine Prophecy (awesome book, btw) back in 1997. A biological research team at Bielefeld University has made a groundbreaking discovery showing that plants can draw an alternative source of energy from other plants. This finding could also have a major impact on the future of bioenergy eventually providing the evidence to show that people draw energy from others in much the same way.. Members of Professor Dr. Olaf Kruses biological research team have confirmed for the first time that a plant, the green alga Chlamydomonas reinhardtii, not only engages in photosynthesis, but also has an alternative source of energy: it can draw it from other plants. The research findings were released this week in the online journal Nature Communications published by the renowned journal Nature.. Flowers need water and light to grow and people are no different. Our physical bodies are like sponges, soaking up the environment. This is exactly why there are certain ...
http://www.loveinactionnow.com/category/health-news-alternative-medicine-hidden-remedies-energy-medicine/page/33/
Plants | Free Full-Text | Regulation by Light of Chemotaxis to Nitrite during the Sexual Life Cycle in Chlamydomonas...Plants | Free Full-Text | Regulation by Light of Chemotaxis to Nitrite during the Sexual Life Cycle in Chlamydomonas...
Nitrite plays an important role in the nitrogen metabolism of most cells, including Chlamydomonas reinhardtii. We have shown that vegetative cells of C. reinhardtii are attracted by nitrite. The Nia1nit2 mutant with defects in genes encoding the nitrate reductase and regulatory protein NIT2 respectively was found to exhibit normal chemotaxis to nitrite. The data suggest that chemotaxis events appear to be specific and independent of those involved in nitrate assimilation. Unlike vegetative cells and noncompetent pregametes, mature gametes did not show chemotaxis to nitrite. Just like gamete formation, the change in chemotaxis mode is controlled by the sequential action of two environmental cues, removal of nitrogen from the medium and light. Comparative analysis of wild-type and RNAi strains with reduced level of phototropin has indicated that switch-off of chemotaxis towards nitrite is dependent on phototropin. The studies revealed individual elements of the phototropin-dependent signal transduction
http://mdpi.com/2223-7747/3/1/113/htm
Chlamydomonas conferenceChlamydomonas conference
The 10th International Conference on the Cell and Molecular Biology of Chlamydomonas will be held in Vancouver, Canada, June 11-16, 2002. Please visit http://www.quarmby.ca/chlamy2002 to register for the meeting and reserve your accommodation. Its going to be a great meeting and we look forward to seeing you all there. A preliminary program outline is now on the program page to facilitate your travel plans. -- Elizabeth Harris chlamy at duke.edu Chlamydomonas Genetics Center home page: http://www.biology.duke.edu/chlamy/ Resource Center for Chlamy genome project: http://www.biology.duke.edu/chlamy_genome/crc.html ------- End of forwarded message ...
http://www.bio.net/bionet/mm/protista/2002-March/001211.html
RABL2A protein (Chlamydomonas reinhardtii) - STRING network viewRABL2A protein (Chlamydomonas reinhardtii) - STRING network view
Small rab-related GTPase; Small GTPase-like component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT25. Has very low GTPase activity either because it lacks the conserved catalytic Gln in position 79 or because it requires some GTPase-activating protein (GAP) for GTP turnover (204 aa ...
https://string-db.org/cgi/network.pl?identifier=3055.EDP00467
RCSB PDB - 7JRJ: Chlamydomonas reinhardtii radial spoke head and neck (recombinant)RCSB PDB - 7JRJ: Chlamydomonas reinhardtii radial spoke head and neck (recombinant)
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/7jrj
Towards functional proteomics of membrane protein complexes: analysis of thylakoid membranes from Chlamydomonas reinhardtii. -...Towards functional proteomics of membrane protein complexes: analysis of thylakoid membranes from Chlamydomonas reinhardtii. -...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/11849598?dopt=Abstract
RCSB PDB - 1FCT: NMR STRUCTURES OF FERREDOXIN CHLOROPLASTIC TRANSIT PEPTIDE FROM CHLAMYDOMONAS REINHARDTII PROMOTED...RCSB PDB - 1FCT: NMR STRUCTURES OF FERREDOXIN CHLOROPLASTIC TRANSIT PEPTIDE FROM CHLAMYDOMONAS REINHARDTII PROMOTED...
1FCT: NMR structures of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution.
http://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1FCT
ORBi: Browsing ORBiORBi: Browsing ORBi
in Molecular and General Genetics (1998), 259(3), 294-8. In Chlamydomonas reinhardtii, mutants defective in the cytochrome pathway of respiration lack the capacity to grow under heterotrophic conditions (in darkness on acetate). In the dark- strain duM18, a + 1 ... [more ▼]. In Chlamydomonas reinhardtii, mutants defective in the cytochrome pathway of respiration lack the capacity to grow under heterotrophic conditions (in darkness on acetate). In the dark- strain duM18, a + 1 T addition in a run of four Ts, located at codon 145 of the mitochondrial cox1 gene encoding subunit I of cytochrome c oxidase, is responsible for the mutant phenotype. A leaky revertant (su11) that grows heterotrophically at a lower rate than wild-type cells was isolated from dum18. Its respiration sensitivity to cyanide was low and its cytochrome c oxidase activity was only 4% of that of the wild-type enzyme. Meiotic progeny obtained from crosses between revertant and wild-type cells inherited the phenotype of the mt- ...
http://orbi.ulg.ac.be/browse?type=author&value=Colin,%20Martine%20p008557
Gametogenesis in the Chlamydomonas reinhardtii minus Mating Type Is Controlled by Two Genes, MID and MTD1 | GeneticsGametogenesis in the Chlamydomonas reinhardtii minus Mating Type Is Controlled by Two Genes, MID and MTD1 | Genetics
This model does not account for two published observations: An mt+ strain carrying the MID gene transposed to an autosome differentiates as minus, as do mt+ cells transformed with the MID gene, even though neither possesses a copy of the MTD1 gene (Ferris and Goodenough 1997). To reconcile these observations with the results reported here, we are led to propose that plus gametes express a system, the MTD1-equivalent system, that is functionally equivalent to the MTD1 system but achieves this outcome without requiring the Mtd1 protein itself. When MID is introduced into a plus background, the MTD1-equivalent system enables sufficiently high MID expression to allow transformants to undergo minus differentiation, albeit success is usually incomplete (see results and Ferris and Goodenough 1997), meaning that the MTD1-equivalent system is not repressible by Mid. Importantly, at least one essential gene in the posited plus MTD-equivalent system must be resident in the MT+ locus. If the system were ...
http://www.genetics.org/content/176/2/913
ATS1 protein (Chlamydomonas reinhardtii) - STRING network viewATS1 protein (Chlamydomonas reinhardtii) - STRING network view
NADPH-dependent flavin reductase; Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe-S cluster of the anamorsin/DRE2 homolog (620 aa ...
https://string-db.org/cgi/network.pl?identifier=3055.EDP03369
Induction and regulation of expression of a low-CO2-induced mitochondrial carbonic anhydrase in Chlamydomonas reinhardtiiInduction and regulation of expression of a low-CO2-induced mitochondrial carbonic anhydrase in Chlamydomonas reinhardtii
The time course of and the influence of light intensity and light quality on the induction of a mitochondrial carbonic anhydrase (CA) in the unicellular green alga Chlamydomonas reinhardtii was characterized using western and northern blots. This CA was expressed only under low-CO2 conditions (ambient air). In asynchronously grown cells, the mRNA was detected 15 min after transfer from air containing 5% CO2 to ambient air, and the 21-kD polypeptide was detected on western blots after 1 h. When transferred back to air containing 5% CO2, the mRNA disappeared within 1 h and the polypeptide was degraded within 3 d. Photosynthesis was required for the induction in asynchronous cultures. The induction increased with light up to 500 mu mol m(-2) s(-1), where saturation occurred. In cells grown synchronously, however, expression of the mitochondrial CA was also detected in darkness. Under such conditions the expression followed a circadian rhythm, with mRNA appearing in the dark 30 min before the light ...
http://umu.diva-portal.org/smash/record.jsf?pid=diva2:434814
Enzymatic properties of the ferredoxin-dependent nitrite reductase from chlamydomonas reinhardtii. Evidence for hydroxylamine...
TY - JOUR. T1 - Enzymatic properties of the ferredoxin-dependent nitrite reductase from chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production. AU - Hirasawa, Masakazu. AU - Tripathy, Jatindra N.. AU - Sommer, Frederik. AU - Somasundaram, Ramasamy. AU - Chung, Jung Sung. AU - Nestander, Matthew. AU - Kruthiventi, Mahima. AU - Zabet-Moghaddam, Masoud. AU - Johnson, Michael K.. AU - Merchant, Sabeeha S.. AU - Allen, James Paul. AU - Knaff, David B.. PY - 2010/1. Y1 - 2010/1. N2 - The ferredoxin-dependent nitrite reductase from the green alga Chlamydomonas reinhardtii has been cloned, expressed in Escherichia coli as a His-tagged recombinant protein, and purified to homogeneity. The spectra, kinetic properties and substrate-binding parameters of the C. reinhardtii enzyme are quite similar to those of the ferredoxin-dependent spinach chloroplast nitrite reductase. Computer modeling, based on the published structure of spinach nitrite reductase, predicts ...
https://sofi-northwestern.pure.elsevier.com/en/publications/enzymatic-properties-of-the-ferredoxin-dependent-nitrite-reductas
Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii | G3: Genes | Genomes | GeneticsRobust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii | G3: Genes | Genomes | Genetics
When Venus was used as a GOI with this system, a positive correlation was found between paromomycin resistance and Venus fluorescence, indicating that expression from the two ORFs is coupled. It initially seemed possible that a mechanism such as stop-codon read through (Jackson et al. 2012) might result in expression of the GOI and APHVIII products as a single fusion protein that provides paromomycin resistance. However, this appears not to be the case, because the method works equally well when two or three different stop codons are inserted between the ORFs, and/or the ORFs are placed out-of-frame, and Western blotting detected no such fusion products. Thus, it seems most likely that APHVIII is translated by post-termination reinitiation (Kozak 2007; Skabkin et al. 2013), in which the ribosome remains associated with the mRNA after termination, continues scanning, and reinitiates translation at a downstream (or, occasionally, upstream: Skabkin et al. 2013) AUG. Such events have been well ...
https://www.g3journal.org/content/6/12/4115
Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed inChlamydomonas reinhardtiichloroplast |...Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed inChlamydomonas reinhardtiichloroplast |...
A Chlamydomonas reinhardtii chloroplast expression vector, pACTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructe
https://link.springer.com/article/10.1023%2FA%3A1024140114505
Mother puts organelles in their place | JCBMother puts organelles in their place | JCB
Like a strict parent, the mother centriole keeps order in the cell by telling other organelles where to sit, according to new work by Jessica Feldman, Wallace Marshall (University of California, San Francisco, CA), and Stefan Geimer (Universität Bayreuth, Bayreuth, Germany).. Marshalls team is interested in what controls the intracellular geometry of organelle positioning. To address this topic, they focus on one organelle that is well-known for its specific positioning: the centriole.. The tethered pair of mother and daughter centrioles is the major component of the centrosome complex and also promotes the assembly of cilia. Thus cilia can act as a cell surface indicator of centriole positioning. The team used the unicellular alga Chlamydomonas reinhardtii, which normally has two cilia at its apex, to scan for mutants in which cilia were misplaced.. In certain misplaced cilia mutants, the fibers that normally tether mother and daughter centrioles were absent. The team found that whereas the ...
http://jcb.rupress.org/content/177/6/946
Phospholipid:diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation...
TY - JOUR. T1 - Phospholipid:diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation while synthesizing triacylglycerol in the unicellular green microalga Chlamydomonas reinhardtii. AU - Yoon, K. AU - Han, D. AU - Li, Y. AU - Sommerfeld, M.. AU - Hu, Q.. AU - Sommerfeld, Milton R. PY - 2012. Y1 - 2012. M3 - Article. SP - 3708. EP - 3724. JO - The Plant Cell. JF - The Plant Cell. ER - ...
https://asu.pure.elsevier.com/en/publications/phospholipiddiacylglycerol-acyltransferase-is-a-multifunctional-e
C2cd3 is required for cilia formation and Hedgehog signaling in mouse | DevelopmentC2cd3 is required for cilia formation and Hedgehog signaling in mouse | Development
Cilia and flagella are cell surface organelles with microtubule-based axonemal cores. Although these organelles have been known to biologists for centuries, only in the last five years has it been recognized that cilia are crucial for mammalian embryonic development as well as for the function of multiple adult organs (Pan et al., 2005). Many potential ciliary proteins have been identified in various species in recent years using biochemical, comparative genomic and proteomic methods. Nevertheless, the spectrum of factors required for the formation and/or function of cilia, as well as the molecular mechanisms underlying the regulation of cilia biogenesis, have yet to be fully revealed.. Two multiprotein complexes, the intraflagellar transport (IFT, complex A and B) complexes, are present in the green alga Chlamydomonas reinhardtii (Rosenbaum and Witman, 2002). The IFT complexes move within the flagella, suggesting that they are likely to be involved in the transportation of molecules inside the ...
http://dev.biologists.org/content/135/24/4049
QUEENS BIOLOGY MCIB SEMINAR SERIES - QUEENS BIOLOGY MCIB SEMINAR SERIESQUEENS BIOLOGY MCIB SEMINAR SERIES - QUEENS BIOLOGY MCIB SEMINAR SERIES
Photosynthetic microalgae hold promise as green cell factories for sustainable light-driven bio-production processes. These organisms can be cultivated with freely available sunlight energy and CO2 as a sole carbon source, making them ideal chassis for sustainable production processes. Microalgae are already natural sources of many interesting bio-products including carotenoids, lipids, and polysaccharides. However, expanding the range as well as value of the compounds produced by microalgae through genetic engineering can increase the economic competitiveness of light-driven algal production platforms. In comparison to bacteria or yeasts, genetic engineering of eukaryotic microalgae has lagged significantly behind due to characteristically low transgene expression levels. Work in our research group has focused on engineering increased and reliable levels of nuclear transgene expression in the fast growing, Chlorophyceaen microalga Chlamydomonas reinhardtii, with the aim of generating ...
https://mcibseminar.weebly.com/home/archives/05-2017
Role of timer and sizer in regulation of Chlamydomonas cell cycle - Fingerprint - Waseda University
Fingerprint Dive into the research topics of Role of timer and sizer in regulation of Chlamydomonas cell cycle. Together they form a unique fingerprint. ...
https://waseda.pure.elsevier.com/en/publications/role-of-timer-and-sizer-in-regulation-of-chlamydomonas-cell-cycle/fingerprints/
recent referencesrecent references
Amoroso, G., D. Sueltemeyer, C. Thyssen and H.P. Fock (1998). Uptake of HCO3- and CO2 in cells and chloroplasts from the microalgae Chlamydomonas reinhardtii and Dunaliella tertiolecta. Plant Physiol. 116, 193-201. Asleson, C.M. and P.A. Lefebvre (1998). Genetic analysis of flagellar length control in Chlamydomonas reinhardtii: A new long-flagella locus and extragenic suppressor mutations. Genetics 148, 693-702. Bhattacharya, D. and L. Medlin (1998). Algal phylogeny and the origin of land plants. Plant Physiol. 116, 9-15. Boschetti, A. and K. Schmid (1998). Energy supply for ATP-synthase deficient chloroplasts of Chlamydomonas reinhardii. Plant Cell Physiol. 39, 160-168. Brosch-Salomon, S., M. Hoeftberger, A. Holzinger and U. Luetz-Meindl (1998). Ultrastructural localization of polysaccharides and N-acetyl-D-galactosamine in the secretory pathway of green algae (Desmidiaceae). J. Exp. Bot. 49, 145-153. Calenberg, M., U. Brohsonn, M. Zedlacher and G. Kreimer (1998). Light- and Ca2+-modulated ...
http://www.bio.net/bionet/mm/chlamy/1998-April/000967.html
UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and...UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and...
Read UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/uv-mediated-chlamydomonas-mutants-with-enhanced-nuclear-transgene-HzDKOMQuI9
RBCS-1 - Ribulose bisphosphate carboxylase small chain 1, chloroplastic precursor - Chlamydomonas reinhardtii - RBCS-1 gene &...RBCS-1 - Ribulose bisphosphate carboxylase small chain 1, chloroplastic precursor - Chlamydomonas reinhardtii - RBCS-1 gene &...
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
https://www.uniprot.org/uniprot/P00873
Biohydrogen - WikipediaBiohydrogen - Wikipedia
In 1933, Marjory Stephenson and her student Stickland reported that cell suspensions catalysed the reduction of methylene blue with H2. Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce hydrogen.[17] In the late 1990s Anastasios Melis discovered that deprivation of sulfur induces the alga to switch from the production of oxygen (normal photosynthesis) to the production of hydrogen. He found that the enzyme responsible for this reaction is hydrogenase, but that the hydrogenase lost this function in the presence of oxygen. Melis also discovered that depleting the amount of sulfur available to the algae interrupted their internal oxygen flow, allowing the hydrogenase an environment in which it can react, causing the algae to produce hydrogen.[18] Chlamydomonas moewusii is also a promising strain for the production of hydrogen.[19][20] ...
https://en.wikipedia.org/wiki/Biological_hydrogen_production
Origin of Polar Order in Dense Suspensions of Phototactic Micro-SwimmersOrigin of Polar Order in Dense Suspensions of Phototactic Micro-Swimmers
A main question for the study of collective motion in living organisms is the origin of orientational polar order, i.e., how organisms align and what are the benefits of such collective behaviour. In the case of micro-organisms swimming at a low Reynolds number, steric repulsion and long-range hydrodynamic interactions are not sufficient to explain a homogeneous polar order state in which the direction of motion is aligned. An external symmetry-breaking guiding field such as a mechanism of taxis appears necessary to understand this phonemonon. We have investigated the onset of polar order in the velocity field induced by phototaxis in a suspension of a motile micro-organism, the algae Chlamydomonas reinhardtii, for density values above the limit provided by the hydrodynamic approximation of a force dipole model. We show that polar order originates from a combination of both the external guiding field intensity and the population density. In particular, we show evidence for a linear dependence of a
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038895
Research Areas
The focus of our groups activities is the analysis and manipulation of metabolic pathways. With an increased understanding of the regulation of metabolism, scientists and engineers can rationally manipulate pathways to produce novel compounds or increase the production of specialty compounds. Quantifying metabolic flux is a critical technology that forms the basis for rational metabolic engineering. Our group has been developing the mathematical modeling and experimental tools for the particularly difficult problem of quantifying fluxes in photoautotrophic organisms. The current focus is on quantifying intracellular metabolite fluxes in cyanobacteria, algae and plants by liquid chromatography-mass spectrometry (LC-MS/MS) and gas chromatography-MS (GC/MS). We have also constructed a genome scale metabolic model of the algae Chlamydomonas reinhardtii and performed flux balance analysis on the network, which we are comparing against experimentally determined metabolic fluxes derived from transient ...
http://web.ics.purdue.edu/~jamorgan/research.htm
What is the nuclear transformation of atoms? | Reference.comWhat is the nuclear transformation of atoms? | Reference.com
Nuclear transformation occurs when the atoms of one element change to become atoms of another element. This is most commonly seen with...
https://www.reference.com/science/nuclear-transformation-atoms-bbabe81992da3853
Phylogenetic and molecular analysis of hydrogen-producing green algae - UQ eSpace
A select set of microalgae are reported to be able to catalyse photobiological H(2) production from water. Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H(2)-producing microalgae. By purging algal cultures with N(2) in the dark and subsequent illumination, it is possible to rapidly induce photobiological H(2) evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H(2). Using this system, five algal isolates capable of H(2) production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H(2) production. H(2)-producing algal species were seen to be ...
https://espace.library.uq.edu.au/view/UQ:181061
anti-Lhcb4 (CP29) plant homolog, Chlamydomonas antibodyanti-Lhcb4 (CP29) plant homolog, Chlamydomonas antibody
Anti-Lhcb4 (CP29) Chlamydomonas reinhardti, Lhcb4, CP29, Lhcbm4 antibody , Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas, Q93WD2, AS06 117
http://www.agrisera.com/en/artiklar/plantalgal-cell-biology/photosynthesis-/lhc/lhcb4-cp29-lhcb4-homolog-chlamydomonas.html
chlamydomonas | Definition of chlamydomonas in English by Oxford Dictionarieschlamydomonas | Definition of chlamydomonas in English by Oxford Dictionaries
Definition of chlamydomonas - a common single-celled green alga which typically has two flagella for swimming, living in water and moist soil.
https://en.oxforddictionaries.com/definition/chlamydomonas
Appropriate observables for investigating narrow resonances in Kaon photoproduction off a proton<...
TY - CHAP. T1 - Appropriate observables for investigating narrow resonances in Kaon photoproduction off a proton. AU - Mart, Terry. PY - 2011/11/1. Y1 - 2011/11/1. N2 - The existence of non-strange partner of pentaquark, the Jp = 1/2+ narrow resonance, has been investigated by utilizing kaon photoproduction off a proton. It is found that the corresponding mass is 1650 MeV and the appropriate observables for investigating the existence of this resonance are the recoiled hyperon polarization, the beam-recoil double polarization Cx, and differential cross section at backward angles. Future kaon photoproduction experiments should focus on these observables.. AB - The existence of non-strange partner of pentaquark, the Jp = 1/2+ narrow resonance, has been investigated by utilizing kaon photoproduction off a proton. It is found that the corresponding mass is 1650 MeV and the appropriate observables for investigating the existence of this resonance are the recoiled hyperon polarization, the beam-recoil ...
https://scholar.ui.ac.id/en/publications/appropriate-observables-for-investigating-narrow-resonances-in-ka
mediaTUM - Media and Publication ServermediaTUM - Media and Publication Server
Engineered tissues are highly limited by poor vascularization in vivo, leading to hypoxia. In order to overcome this challenge, we propose the use of photosynthetic biomaterials to provide oxygen. Since photosynthesis is the original source of oxygen for living organisms, we suggest that this could be a novel approach to provide a constant source of oxygen supply independently of blood perfusion. In this study we demonstrate that bioartificial scaffolds can be loaded with a solution containing the photosynthetic microalgae Chlamydomonas reinhardtii, showing high biocompatibility and photosynthetic activity in vitro. Furthermore, when photosynthetic biomaterials were engrafted in a mouse full skin defect, we observed that the presence of the microalgae did not trigger a native immune response in the host. Moreover, the analyses showed that the algae survived for at least 5days in vivo, generating chimeric tissues comprised of algae and murine cells. The results of this study represent a crucial ...
http://mediatum.ub.tum.de/1322629
Lab Technician (Ref 06/2020) | CRAGJobsLab Technician (Ref 06/2020) | CRAGJobs
ABOUT CRAG:. CRAG is an independent research institution engaged in leading-edge basic and applied plant and farm animal sciences. CRAG is established as a Consortium of the Spanish National Research Council (CSIC), Institute of Agrifood Research and Technology (IRTA), Autonomous University of Barcelona (UAB), and University of Barcelona (UB). The Center is located at the UAB Campus, and currently hosts 200 members from across the world.. Research Programs at CRAG (from basic science to applied research using plant experimental model systems, crops and farm animals) make extensive use of genomic technologies and large sets of genetic and genomic data (https://biennialreport2016-2017.cragenomica.es/).. RESPONSIBILITIES:. The hired technician will assist in undergoing research aimed at characterizing the components of light, high light and retrograde signaling in the model organisms Arabidopsis thaliana and Chlamydomonas reinhardtii.. The tasks are included within the framework of the project ...
https://recruitment.cragenomica.es/jobs/lab-technician-ref-062020
Structural basis of light-induced redox regulation in the Calvin-Benson cycle in cyanobacteria | PNASStructural basis of light-induced redox regulation in the Calvin-Benson cycle in cyanobacteria | PNAS
In the GAPDH-CP12-PRK complex, T. elongatus PRK is dimeric, as in solution (SI Appendix, Fig. S5E), and like other plant-type PRKs (6, 7). PRK has an alpha-beta-alpha sandwich fold where the central 9-strand beta-sheet is continuous across the dimer interface (Fig. 3C). The active site cleft lies between 3 loops (residues 137-164, 43-63, and 87-98). The N-terminal helical bundle of CP12 plugs this cleft and sterically blocks the active site (Fig. 3D). The charged patch created by the CP12 motif binds to complementary positively charged regions in the PRK active site (Fig. 3E), which have also been proposed to be important for negatively charged sugar phosphate substrate binding (6). Variants in the CP12 of Chlamydomonas reinhardtii, equivalent to Trp33, Glu37, and Glu38 in the conserved CP12 motif resulted in loss of complex formation (24). The conserved CP12-Trp33 is on the surface and packs against PRK, contradicting a prediction that it is buried (29). CP12-Glu33 makes a salt bridge with ...
https://www.pnas.org/content/116/42/20984
Tutorial - MapManTutorial - MapMan
May, P., Wienkoop, S., Kempa, S., Usadel, B., Christian, N., Rupprecht, J., Weiss, J., Recuenco-Munoz, L., Ebenhöh, O., Weckwerth, W., Walther, D (2008) Metabolomics- and proteomics-assisted genome annotation and analysis of the draft metabolic network of Chlamydomonas reinhardtii. Genetics. 179:157-66.. Howell, K.A., Narsai, R., Carroll, A., Ivanova, A., Lohse, M., Usadel, B., Millar, A.H., Whelan, J. (200X) Mapping metabolic and transcript temporal switches during germination in Oryza sativa highlights specific transcription factors and the role of RNA instability in the germination process. Plant Physiol. accepted. Crowhurst, R.N., et al. (2008) Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening. BMC Genomics. 9:351.. ...
http://mapman.gabipd.org/iw/tutorial
Youngsook Lee, Department of Life Sciences, Pohang University of Science and Technology - PhD.GO
Biology, Botany, Biochemistry, Cell biology, Arabidopsis, ATP-binding cassette transporter, Arabidopsis thaliana, Mutant, Genetics, Chlamydomonas reinhardtii,
https://phdgo.com/professor/3339/
UTEX 969 Chlamydomonas applanata | UTEX Culture Collection of AlgaeUTEX 969 Chlamydomonas applanata | UTEX Culture Collection of Algae
Algae Detail UTEX Number: 969Class: ChlorophyceaeStrain: Chlamydomonas applanataMedia: Modified Bold 3N MediumOrigin: Williamson Co., Texas, USADescription of L
https://utex.org/products/utex-0969
UTEX LB 943 Chlamydomonas gigantea | UTEX Culture Collection of AlgaeUTEX LB 943 Chlamydomonas gigantea | UTEX Culture Collection of Algae
Algae Detail UTEX Number: 943Class: ChlorophyceaeStrain: Chlamydomonas giganteaMedia: Modified Bold 3N MediumOrigin: Description of Location: Type Culture: NoCo
https://utex.org/products/utex-lb-0943
Nucleus-basal body connector in chlamydomonas: Evidence for a role in basal body segregation and against essential roles in...Nucleus-basal body connector in chlamydomonas: Evidence for a role in basal body segregation and against essential roles in...
Fingerprint Dive into the research topics of Nucleus-basal body connector in chlamydomonas: Evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Together they form a unique fingerprint. ...
https://experts.umn.edu/en/publications/nucleus-basal-body-connector-in-chlamydomonas-evidence-for-a-role/fingerprints/
User:Adam Katzenberg/Notebook/Biology 210 at AU - OpenWetWareUser:Adam Katzenberg/Notebook/Biology 210 at AU - OpenWetWare
Introduction Bio 2010 01/31/2014 The objectives of this lab were to understand natural selection and understand the biotic and abiotic characteristics of a niche. In order to understand natural selection in this lab we observed characteristics of three different algae families; Volvox, Chlamydomonas, and Gonium. By observing these algae will give us a better understanding of how a simple cellular organism evolved due to natural selection and are able to survive today. These organisms will give us context into natural selection occurring on bigger organisms as we move on in lab. Similarly, in order to understand biotic and abiotic characteristics of a niche, we observed a transect. By observing a transect will provide insight into how the biosphere works by focussing on biotic and biotic components of a home to many organisms. Procedure 1: The Volvicine Line In this experiment we observed an isogamous, single celled, motile alga called chlamydomonas. We prepared a slide of a living Chlamydomonas ...
https://openwetware.org/wiki/?title=User:Adam_Katzenberg/Notebook/Biology_210_at_AU&curid=148269&oldid=790902
Category:MichSt14A 13 - GONUTSCategory:MichSt14A 13 - GONUTS
Figure 5A. Wildtype and roc15 mutant Chlamydomonas was entrained to a 12:12 light:dark cycle. The cultures were transferred to constant darkness and their tufA expression was measured with luminescence. They were then expressed to 5 minute long pulses of light. The wild type Chlamydomonas responded to the light pulses, evident by a phase shift in tufA expression. The roc15 mutant Chlamydomonas did not exhibit this phase shift. This indicate that roc15 is essential for circadian regulated phase shifts and entrainment. ...
https://gowiki.tamu.edu/wiki/index.php/Category:MichSt14A_13
Nordic Cilia and Centrosome Network
Immunofluorescence micrograph of a Chlamydomonas cell stained with acetylated tubulin antibody to label flagella (green) and with an antibody against the protein EB1 that labels the flagella tip and base (red/yellow). Image from Lotte Pedersen, University of Copenhagen. To previous page ...
http://nordiccilia.org/pix/1.asp
Positive Tested Species Reactivity: Canine, Chlamydomonas, Recommended Applications: FFPE, Function-BlockingPositive Tested Species Reactivity: Canine, Chlamydomonas, Recommended Applications: FFPE, Function-Blocking
The Cytoskeleton Collection includes 250 mAbs recognizing the supportive structural filaments and associated proteins that facilitate cell shape and motility
https://dshb.biology.uiowa.edu/collections/cytoskeleton/custitem34/Canine,Chlamydomonas/custitem40/FFPE,Function-Blocking
Positive Tested Species Reactivity: Chlamydomonas, Snake, Recommended Applications: ELISA, ImmunohistochemistryPositive Tested Species Reactivity: Chlamydomonas, Snake, Recommended Applications: ELISA, Immunohistochemistry
The Cytoskeleton Collection includes 250 mAbs recognizing the supportive structural filaments and associated proteins that facilitate cell shape and motility
https://dshb.biology.uiowa.edu/collections/cytoskeleton/custitem34/Chlamydomonas,Snake/custitem40/ELISA,Immunohistochemistry
Alphabetic Listing of Presenting Authors - R | Chlamydomonas GeneticsAlphabetic Listing of Presenting Authors - R | Chlamydomonas Genetics
Alphabetic Listing of Presenting Authors - R. If you have any questions or comments then please send an email to [email protected] ...
http://www.genetics-gsa.org/chlamydomonas/2014/abstracts/assignments/R.shtml
Sequence DetailSequence Detail
pep:novel chromosome:VEGA66:10:33905485:33915883:1 gene:OTTMUSG00000029742 transcript:OTTMUST00000073807 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Rsph4a description:radial spoke head 4 homolog A (Chlamydomonas ...
http://www.informatics.jax.org/sequence/OTTMUSP00000038252
What is the habitat of a green algae plant? | Reference.comWhat is the habitat of a green algae plant? | Reference.com
All algae need access to light and live in oxygenated water. There are more than 7,000 species of green algae, which live in a variety of...
https://www.reference.com/science/habitat-green-algae-plant-49bf5389b548dd4d
Peta Grand Pesona (tempat retret) « Pria Sejati KatolikPeta Grand Pesona (tempat retret) « Pria Sejati Katolik
Mohon maaf, kami belum menyediakan peta untuk retret 21-22 Juli ini. Peta yang Bapak lihat adalah peta tempat retret yang sebelumnya ...
https://priasejatikatolik.org/2012/03/22/peta-grand-pesona-tempat-retret/
PETA Statement: Animal Dealers Sentence Is a Step in the Right Direction | PETAPETA Statement: Animal Dealers Sentence Is a Step in the Right Direction | PETA
Below, please find a statement from PETA Senior Vice President Daphna Nachminovitch in response to todays sentencing of Jurassic Pets Manager Brian
https://www.peta.org/media/news-releases/peta-statement-animal-dealers-sentence-step-right-direction/
Green Alga / Zelena alga | Project NoahGreen Alga / Zelena alga | Project Noah
Project Noah is a tool that nature lovers can use to explore and document local wildlife and a common technology platform that research groups can use to harness the power of citizen scientists everywhere.
https://www.projectnoah.org/spottings/36857003
Great Starbucks News! | PETAGreat Starbucks News! | PETA
ThisNext/Creative CommonsThis little piece of good news comes to you courtesy of my friend (and occasional PETA Files blogger) Joel Bartlett, who spends
https://www.peta.org/blog/great-starbucks-news/
Telescoape astronomice Celestron seria CGE PRO - Schmidt-Cassegrain clasice si Edge HD
Telescoape astronomice Celestron CGE PRO semiprofesionale SCT clasice si Edge HD pentru observatoare astronomice si cercetare, telescop astronomic Celestron CGE PRO 925 1100 1400 Edge HD
http://starmax.ro/cgepro_cel.htm
Chlamydomonas reinhardtiiChlamydomonas reinhardtii
"Home - Chlamydomonas reinhardtii v3.0". "Chlamydomonas reinhardtii mitochondrion, complete genome". February 2010. {{cite ... Chlamydomonas species are widely distributed worldwide in soil and fresh water. Chlamydomonas reinhardtii is an especially well ... Protist locomotion#Biohybrid microswimmers D66 strain of Chlamydomonas reinhardtii "CC-125 wild type mt+ 137c". Chlamydomonas ... and reinhardtii all refer to the same species, C. reinhardtii Dangeard. Chlamydomonas is used as a model organism for research ...
https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii
D66 strain of Chlamydomonas reinhardtiiD66 strain of Chlamydomonas reinhardtii
The D66 strain of Chlamydomonas reinhardtii, a single-celled green alga, is a cell-wall-deficient strain of algae that exhibits ... The D66 strain of Chlamydomonas reinhardtii has been genetically engineered with no cell wall in order to increase the strain's ... Adams, James (May 2004). "MOLECULAR, GENETIC AND PHYSIOLOGICAL CHARACTERIZATION OF A CHLAMYDOMONAS REINHARDTII INSERTIONAL ... "Rubisco Activase is Required for Optimal Photosynthesis in the Green Alga Chlamydomonas reinhardtii in a Low-CO2 Atmosphere". ...
https://en.wikipedia.org/wiki/D66_strain_of_Chlamydomonas_reinhardtii
FlagellumFlagellum
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-7. doi:10.1104/pp.010807. PMC 1540183. PMID 11743094. ...
https://en.wikipedia.org/wiki/Flagellum
Plant cellPlant cell
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
https://en.wikipedia.org/wiki/Plant_cell
CentrioleCentriole
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
https://en.wikipedia.org/wiki/Centriole
EukaryoteEukaryote
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
https://en.wikipedia.org/wiki/Eukaryote
Evans blue (dye)Evans blue (dye)
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
https://en.wikipedia.org/wiki/Evans_blue_(dye)
Viability assayViability assay
Crutchfield A, Diller K, Brand J (1999-02-01). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal ...
https://en.wikipedia.org/wiki/Viability_assay
UVR8UVR8
Chlamydomonas reinhardtii and Volvox carteri). The latter implies that UVR8 potentially appeared before the evolutionary split ...
https://en.wikipedia.org/wiki/UVR8
MatC familyMatC family
Chlamydomonas reinhardtii) and archaea (e.g., Methanococcus jannaschii). The proteins are of about 450 amino acyl residues in ...
https://en.wikipedia.org/wiki/MatC_family
BioCyc database collectionBioCyc database collection
based on the Chlamydomonas reinhardtii genome. It has 866 unique ORFs, 1862 metabolites, 2499 gene-enzyme-reaction-association ... a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome". BMC Genomics. 12 Suppl 4: S5. ...
https://en.wikipedia.org/wiki/BioCyc_database_collection
Phosphoglycolate phosphatasePhosphoglycolate phosphatase
Ensuing studies 20 years after the identification of the same mutant strain of Chlamydomonas reinhardtii found that the ... When a photorespiratory mutant of the eukaryotic green alga Chlamydomonas reinhardtii was studied, the mutant strain was ... Suzuki K, Marek LF, Spalding MH (May 1990). "A photorespiratory mutant of Chlamydomonas reinhardtii". Plant Physiology. 93 (1 ... "Characteristics and sequence of phosphoglycolate phosphatase from a eukaryotic green alga Chlamydomonas reinhardtii". The ...
https://en.wikipedia.org/wiki/Phosphoglycolate_phosphatase
Furoquinoline alkaloidFuroquinoline alkaloid
Schimmer, O; Kühne, I (1991). "Furoquinoline alkaloids as photosensitizers in Chlamydomonas reinhardtii". Mutation Research. ...
https://en.wikipedia.org/wiki/Furoquinoline_alkaloid
Biohybrid microswimmerBiohybrid microswimmer
Chlamydomonas reinhardtii is a unicellular green microalga. The wild-type C. reinhardtii has a spherical shape that averages ... C. reinhardtii has been actively explored as the live component of biohybrid microrobots for the active delivery of ... Alternative attachment strategies for C. reinhardtii have been proposed for the assembly through modifying the interacting ...
https://en.wikipedia.org/wiki/Biohybrid_microswimmer
Silver nanoparticleSilver nanoparticle
December 2008). "Toxicity of silver nanoparticles to Chlamydomonas reinhardtii". Environmental Science & Technology. 42 (23): ...
https://en.wikipedia.org/wiki/Silver_nanoparticle
Morn repeat containing 1Morn repeat containing 1
Chlamydomonas reinhardtii] - Protein - NCBI". "Radial spoke head component 1 L homeolog [Xenopus laevis] - Protein - NCBI". " ...
https://en.wikipedia.org/wiki/Morn_repeat_containing_1
PyrenoidPyrenoid
The discovery of pyrenoid deficient mutants with normal starch grains in the green alga Chlamydomonas reinhardtii, as well as ... Moroney, J. V., & Ynalvez, R. A. (2007). Proposed carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii. ... reinhardtii pyrenoids in vivo, further supporting a "linker" role for EPYC1. The proteome of the Chlamydomonas pyrenoid has ... in the green alga Chlamydomonas reinhardtii, multiple thylakoids merge at the periphery of the pyrenoid to form larger tubules ...
https://en.wikipedia.org/wiki/Pyrenoid
Model organismModel organism
Chlamydomonas reinhardtii, a unicellular green alga with well-studied genetics, is used to study photosynthesis and motility. C ... "Chlamydomonas reinhardtii resources at the Joint Genome Institute". Archived from the original on 2008-07-23. Retrieved 2007-10 ... Batyrova, Khorcheska; Hallenbeck, Patrick C. (2017-03-16). "Hydrogen Production by a Chlamydomonas reinhardtii Strain with ... reinhardtii has many known and mapped mutants and expressed sequence tags, and there are advanced methods for genetic ...
https://en.wikipedia.org/wiki/Model_organism
PhototaxisPhototaxis
In the best-studied green alga, Chlamydomonas reinhardtii, phototaxis is mediated by a rhodopsin pigment, as first demonstrated ... and high-intensity light in Chlamydomonas reinhardtii". Proceedings of the National Academy of Sciences. 99 (13): 8689-8694. ... "Ciliary behavior of a negatively phototactic Chlamydomonas reinhardtii". Cell Motility and the Cytoskeleton. 61 (2): 97-111. ... "Linear systems analysis of the ciliary steering behavior associated with negative-phototaxis in Chlamydomonas reinhardtii". ...
https://en.wikipedia.org/wiki/Phototaxis
Sodium sulfate symporterSodium sulfate symporter
Chlamydomonas reinhardtii putative sulphur deprivation response regulator SAC1. This family also includes a number of bacterial ... a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation". EMBO J. 15 (9): ...
https://en.wikipedia.org/wiki/Sodium_sulfate_symporter
Sabeeha MerchantSabeeha Merchant
She worked alongside Bruce Selman on the single-cell algae Chlamydomonas reinhardtii. Chlamydomonas reinhardtii is a model ... Merchant was the first to demonstrate that the RNA for Chlamydomonas reinhardtii plastocyanin is produced when copper is ... "Studies on chloroplast development in Chlamydomonas reinhardtii IV. Control of rapid chlorophyll formation in greening y-1 ... crystal structure of plastocyanin from the green alga Chlamydomonas reinhardtii". Biochemistry. 32 (40): 10560-10567. doi: ...
https://en.wikipedia.org/wiki/Sabeeha_Merchant
List of model organismsList of model organisms
Sequencing of the Chlamydomonas reinhardtii genome was reported in October 2007. A Chlamydomonas genetic stock center exists at ... Chlamydomonas reinhardtii has well-studied genetics, with many known and mapped mutants and expressed sequence tags, and there ... "Chlamydomonas reinhardtii resources at the Joint Genome Institute". Archived from the original on 23 July 2008. Retrieved 1 ... Chlamydomonas reinhardtii, unicellular green alga used to study photosynthesis, flagella and motility, regulation of metabolism ...
https://en.wikipedia.org/wiki/List_of_model_organisms
Prachee AvasthiPrachee Avasthi
Avasthi uses Chlamydomonas reinhardtii, a unicellular green alga, to investigate the assembly of cilia. She was particularly ... She works on upwardly motile Chlamydomonas reinhardtii and is on the Board of Directors of eLife. Avasthi studied integrative ... Here she began work on Chlamydomonas reinhardtii, a model organism for studying cilia. Cilia function requires normal cilia ... September 2014). "Actin is required for IFT regulation in Chlamydomonas reinhardtii". Current Biology. 24 (17): 2025-32. doi: ...
https://en.wikipedia.org/wiki/Prachee_Avasthi
ChlororespirationChlororespiration
However, experimentation on Chlamydomonas reinhardtii, discovered Plastoquinone (PQ) to be a redox carrier. The role of this ... Peltier, G.; Schmidt, G. W. (1991). "Chlororespiration: an adaptation to nitrogen deficiency in Chlamydomonas reinhardtii". ... The mutant Chlamydomonas plant species, lacks photosystems one and two (PS I and PS II), so when the plant underwent flash- ... Evidence using mass spectrometry on algae and photosynthetic mutants of Chlamydomonas, discovered that oxygen molecules were ...
https://en.wikipedia.org/wiki/Chlororespiration
I-CreII-CreI
... is a homing endonuclease whose gene was first discovered in the chloroplast genome of Chlamydomonas reinhardtii, a ... Seligman, LM; Stephens, KM; Savage, JH; Monnat, RJ (1997). "Genetic Analysis of the Chlamydomonas reinhardtii I-CreI Mobile ... Rochaix, JD; Malnoe, P (1978). "Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardtii". Cell. 15 (2): 661-670. ... Dürrenberger F, Rochaix JD (November 1991). "Chloroplast ribosomal intron of Chlamydomonas reinhardtii: in vitro self-splicing ...
https://en.wikipedia.org/wiki/I-CreI
Francis-André WollmanFrancis-André Wollman
Polypeptide phosphorylation in Chlamydomonas reinhardtii », ( 1984) 98, j. cell. biol., p. 1-7 Wollman F-A., « State ... Lateral distribution of the main protein complexes of the photosynthetic apparatus in Chlamydomonas reinhardtii and in spinach ... an approach using genetic transformation of the green alga Chlamydomonas reinhardtii », EMBO J., (1994), 13(5), p. 1019-27 ... Using the power of the genetic approach in a microalgae, Chlamydomonas reinhartdii, he combined biophysical, biochemical and ...
https://en.wikipedia.org/wiki/Francis-André_Wollman
BiohydrogenBiohydrogen
"An energy balance from absorbed photons to new biomass for Chlamydomonas reinhardtii and Chlamydomonas acidophila under neutral ... In Chlamydomonas reinhardtii Photosystem II produces in direct conversion of sunlight 80% of the electrons that end up in the ... Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce ... "Truncated Photosystem Chlorophyll Antenna Size in the Green Microalga Chlamydomonas reinhardtii upon Deletion of the TLA3- ...
https://en.wikipedia.org/wiki/Biohydrogen
Stokes radiusStokes radius
"Purification and Molecular Properties of Urate Oxidase from Chlamydomonas Reinhardtii". Biochimica et Biophysica Acta (BBA) - ...
https://en.wikipedia.org/wiki/Stokes_radius
Solar fuelSolar fuel
Some of these organisms produce hydrogen upon switching culture conditions; for example, Chlamydomonas reinhardtii produces ... "Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: Effects of culture parameters". Biotechnology and ...
https://en.wikipedia.org/wiki/Solar_fuel
Endoplasmic reticulum membrane protein complexEndoplasmic reticulum membrane protein complex
Mitra M, Melis A (February 2010). "Genetic and biochemical analysis of the TLA1 gene in Chlamydomonas reinhardtii". Planta. 231 ... "Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA ...
https://en.wikipedia.org/wiki/Endoplasmic_reticulum_membrane_protein_complex
Mitochondrial DNAMitochondrial DNA
In many unicellular organisms (e.g., the ciliate Tetrahymena and the green alga Chlamydomonas reinhardtii), and in rare cases ...
https://en.wikipedia.org/wiki/Mitochondrial_DNA
WNK1WNK1
Chlamydomonas reinhardtii and Vitis viniferaas well as in vertebrates including Danio rerio and Taeniopygia guttata. GRCh38: ...
https://en.wikipedia.org/wiki/WNK1
Ribose-seqRibose-seq
2021). "Disproportionate presence of adenosine in mitochondrial and chloroplast DNA of Chlamydomonas reinhardtii". iScience. 24 ... and chloroplast genomes of Chlamydomonas reinhardtii, a photosynthetic unicellular green alga. In mitochondrial and chloroplast ... In all C. reinhardtii organelles, rU was consistently the least represented rNMP, which is consistent with findings from yeast ... Similar to yeast profiles, the incorporation of rNMPs in C. reinhardtii is most impacted by the dNMP immediately upstream ( ...
https://en.wikipedia.org/wiki/Ribose-seq
BioGRIDBioGRID
Chlamydomonas reinhardtii (green algae) Chlorocebus sabaeus (green monkey) Cricetulus griseus (Chinese hamster) Danio rerio ( ...
https://en.wikipedia.org/wiki/BioGRID
AutolysinAutolysin
... of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases". European Journal of Biochemistry. 170 (1-2): 485-491. ... gelatin and Leu-Trp-Met-Arg-Phe-Ala This glycoprotein is present in Chlamydomonas reinhardtii gametes. Gram-positive bacteria ... during sexual signalling in Chlamydomonas: the enzyme is stored as an inactive, higher relative molecular mass precursor in the ... and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, ...
https://en.wikipedia.org/wiki/Autolysin
DNAI1DNAI1
"Loss-of-function mutations in a human gene related to Chlamydomonas reinhardtii dynein IC78 result in primary ciliary ...
https://en.wikipedia.org/wiki/DNAI1
PhotoinhibitionPhotoinhibition
ISBN 978-0-521-64497-6. Tibiletti, T., Auroy, P., Peltier, G. and Caffarri, S. (2016). Chlamydomonas reinhardtii PsbS protein ... Chlamydomonas reinhardtii. Their data concluded that the PsBs protein belongs to a multigene family termed LhcSR proteins, ...
https://en.wikipedia.org/wiki/Photoinhibition
Eva-Mari AroEva-Mari Aro
Chlamydomonas reinhardtii, diatoms, Physcomitrella patens, ferns, Arabidopsis thaliana, and spruce. Her applied research ...
https://en.wikipedia.org/wiki/Eva-Mari_Aro
BtpA proteinBtpA protein
"The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the ...
https://en.wikipedia.org/wiki/BtpA_protein
Cytochrome b559Cytochrome b559
"Mössbauer studies of the non-heme iron and cytochrome b559 in a Chlamydomonas reinhardtii PSI- mutant and their interactions ...
https://en.wikipedia.org/wiki/Cytochrome_b559
CellulaseCellulase
Recently, cellulases have also been found in green microalgae (Chlamydomonas reinhardtii, Gonium pectorale and Volvox carteri) ...
https://en.wikipedia.org/wiki/Cellulase
Edible algae vaccineEdible algae vaccine
... analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii". Plant Biotechnology Journal. 8 ... The vaccine was grown in C. reinhardtii algae and provided oral vaccination in mice, but was hindered by low vaccine antigen ... research to combine a genetically engineered sub-unit vaccine and an immunologic adjuvant into Chlamydomonas reinhardtii ...
https://en.wikipedia.org/wiki/Edible_algae_vaccine
Daniel Colón-RamosDaniel Colón-Ramos
There, he studied how the architecture of the nucleus of the algae Chlamydomonas reinhardtii changes to affect cytoplasmic ... distribution of nuclear pore complexes and the cytoplasmic localization of beta2-tubulin mRNA in Chlamydomonas reinhardtii". ...
https://en.wikipedia.org/wiki/Daniel_Colón-Ramos
RSPH4ARSPH4A
... as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. Radial ... "Entrez Gene: radial spoke head 4 homolog A (Chlamydomonas)". Castleman VH, Romio L, Chodhari R, Hirst RA, de Castro SC, Parker ...
https://en.wikipedia.org/wiki/RSPH4A
BotanyBotany
The single celled green alga Chlamydomonas reinhardtii, while not an embryophyte itself, contains a green-pigmented chloroplast ... The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas. Dordrecht, Germany: Kluwer Academic. ISBN 978-0-7923- ...
https://en.wikipedia.org/wiki/Botany
PaucimannosylationPaucimannosylation
"Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtii Unravels Novel Complex Structures". Molecular & Cellular ...
https://en.wikipedia.org/wiki/Paucimannosylation
ReproductionReproduction
For example, in the green alga, Chlamydomonas reinhardtii, there are so-called "plus" and "minus" gametes. A few types of ...
https://en.wikipedia.org/wiki/Reproduction
History of research on Arabidopsis thalianaHistory of research on Arabidopsis thaliana
... model alga Chlamydomonas reinhardtii, and soybean as its "flagship" species for plant genomics geared towards bioenergy ... thaliana along with the alga Chlamydomonas reinhardtii.) Prior to this, a handful of A. thaliana geneticists had become HHMI ... Gutman, Benjamin L.; Niyogi, Krishna K. (2004-06-01). "Chlamydomonas and Arabidopsis. A Dynamic Duo". Plant Physiology. 135 (2 ...
https://en.wikipedia.org/wiki/History_of_research_on_Arabidopsis_thaliana
Lynne QuarmbyLynne Quarmby
Through her research using the single-celled ciliated green alga Chlamydomonas reinhardtii as a model organism, Quarmby ... Finst, Rip J.; Kim, Peter J.; Quarmby, Lynne M. (1998). "Genetics of the deflagellation pathway in Chlamydomonas". Genetics. ... "The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during ...
https://en.wikipedia.org/wiki/Lynne_Quarmby
ERG3ERG3
"Functional characterization of the Chlamydomonas reinhardtii ERG3 ortholog, a gene involved in the biosynthesis of ergosterol ...
https://en.wikipedia.org/wiki/ERG3
Ernst BambergErnst Bamberg
With Georg Nagel and Peter Hegemann, who were attempting to identify the proteins that allow Chlamydomonas reinhardtii, a green ...
https://en.wikipedia.org/wiki/Ernst_Bamberg
PhotorespirationPhotorespiration
"The induction of the CO2 concentrating mechanism in a starch-less mutant of Chlamydomonas reinhardtii". Physiologia Plantarum. ...
https://en.wikipedia.org/wiki/Photorespiration
Sedoheptulose-bisphosphataseSedoheptulose-bisphosphatase
... of a potential redox-sensitive interdomain disulfide in the sedoheptulose bisphosphatase of Chlamydomonas reinhardtii". The ...
https://en.wikipedia.org/wiki/Sedoheptulose-bisphosphatase
Respiratory complex IRespiratory complex I
"Higher plant-like subunit composition of mitochondrial complex I from Chlamydomonas reinhardtii: 31 conserved components among ...
https://en.wikipedia.org/wiki/Respiratory_complex_I
Dunaliella salinaDunaliella salina
Species in the genus Dunaliella are morphogically similar to Chlamydomonas reinhardtii with the main exception being that ...
https://en.wikipedia.org/wiki/Dunaliella_salina
Molecular Characterization of Chlamydomonas Reinhardtii, ac-208: A Plastocyanin-Less Mutant - CaltechAUTHORSMolecular Characterization of Chlamydomonas Reinhardtii, ac-208: A Plastocyanin-Less Mutant - CaltechAUTHORS
Chlamydomonas reinhardtii, ac-208, is an acetate-requiring mutant which was isolated on the basis of its inability to fix ... Molecular Characterization of Chlamydomonas Reinhardtii, ac-208: A Plastocyanin-Less Mutant. In: Progress in Photosynthesis ...
https://authors.library.caltech.edu/57255/
Chlamydomonas reinhardtiiChlamydomonas reinhardtii
... Scientific classification Kingdom: Protistae Division: Chlorophyta Class: ... Chlamydomonas reinhardtii. P.A.Dang. Chlamydomonas reinhardtii is a motile single celled green alga about 10 micrometres in ... C. reinhardtii as a model organism. Chlamydomonas is used as a model organism for research on fundamental questions in cell and ... Blue- and red-light regulation of the cell cycle in Chlamydomonas reinhardtii (Chlorophyta). Eur. J. Phycol. 41: 313 - 320 ...
https://www.bionity.com/en/encyclopedia/Chlamydomonas_reinhardtii.html
Transcriptome-Wide Changes in <i>Chlamydomonas reinhardtii</i> Gene Ex by Wei Fang,..."Transcriptome-Wide Changes in <i>Chlamydomonas reinhardtii</i> Gene Ex" by Wei Fang,...
Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Expression Regulated by Carbon Dioxide and the CO2-Concentrating ...
https://digitalcommons.unl.edu/statisticsfacpub/13/
Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii | EPICOsmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii | EPIC
Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii Luykx, P., Hoppenrath, ... Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii , Protoplasma, 200 , pp ...
https://epic.awi.de/id/eprint/8487/
Figures and data in The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas...Figures and data in The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas...
... reinhardtii has profoundly advanced many areas of biology, but much remains to be learnt about its life in the wild. ... Structure of a vegetative Chlamydomonas reinhardtii cell.. This cell has a 5-10 µm diameter (Gallaher et al., 2015). The two ... The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in ... The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in ...
https://elifesciences.org/articles/39233/figures
Concerted Up-regulation of Aldehyde/Alcohol Dehydrogenase (ADHE) and Starch in Chlamydomonas reinhardtii Increases Survival...
... the first molecular and enzymatic characterization of the ADHE from the photosynthetic microalga Chlamydomonas reinhardtii. ... Concerted Up-regulation of Aldehyde/Alcohol Dehydrogenase (ADHE) and Starch in Chlamydomonas reinhardtii Increases Survival ... and Starch in Chlamydomonas reinhardtii Increases Survival under Dark Anoxia. Journal of Biological Chemistry, 2017, 292 (6), ... Keywords : metalloprotein algae metabolic regulation zinc western blot chloroplast Chlamydomonas carbohydrate metabolism ...
https://hal-amu.archives-ouvertes.fr/hal-01430308
The function of PROTOPORPHYRINOGEN IX OXIDASE in chlorophyll biosynthesis requires oxidised plastoquinone in Chlamydomonas...The function of PROTOPORPHYRINOGEN IX OXIDASE in chlorophyll biosynthesis requires oxidised plastoquinone in Chlamydomonas...
... reinhardtii$. The PPX-PQ pool interaction is proposed to function as a feedback loop between photosynthetic electron transport ... This observation strongly indicates the need of oxidised PQ as the electron acceptor for the PPX reaction in $Chlamydomonas\ ... This observation strongly indicates the need of oxidised PQ as the electron acceptor for the PPX reaction in $Chlamydomonas\ ... of PROTOPORPHYRINOGEN IX OXIDASE in chlorophyll biosynthesis requires oxidised plastoquinone in Chlamydomonas reinhardtii Pawel ...
https://hal-cea.archives-ouvertes.fr/cea-02149191
RPG: Chlamydomonas reinhardtii - RPL38RPG: Chlamydomonas reinhardtii - RPL38
Chlamydomonas reinhardtii - RPL38. Species. Chlamydomonas reinhardtii. Gene Name. RPL38. 5 Upstream. ...
http://ribosome.med.miyazaki-u.ac.jp/rpg.cgi?mode=strc&id=CRE10073
Intraflagellar Transport In Chlamydomonas ReinhardtiiIntraflagellar Transport In Chlamydomonas Reinhardtii
Chlamydomonasreinhardtii. Biochemical analysis of the 17 proteins found in the Chlamydomonas IFT particles has begun toreveal ...
https://vivo.nkn.uidaho.edu/vivo/display/nih11
Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan<...
Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. In: Journal of Plant Research. ... Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. Journal of Plant Research. 2010 ... Chlamydomonas reinhardtii P. A. Dang. (Volvocales, Chlorophyceae) is one of the most intensely studied algae, and its whole ... N2 - Chlamydomonas reinhardtii P. A. Dang. (Volvocales, Chlorophyceae) is one of the most intensely studied algae, and its ...
https://keio.pure.elsevier.com/en/publications/recharacterization-of-chlamydomonas-reinhardtii-and-its-relatives
How does eukaryotic gene prediction work? | Nature BiotechnologyHow does eukaryotic gene prediction work? | Nature Biotechnology
Comparison of the chloroplast peroxidase system in the chlorophyte Chlamydomonas reinhardtii, the bryophyte Physcomitrella ...
https://www.nature.com/articles/nbt0807-883?error=cookies_not_supported&code=fc7e8f2f-b76b-4818-ac02-6916083f39c5
Effect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas ReinhardtiiEffect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas Reinhardtii
Chlamydomonas reinhardtii; Lipid content; Triacylglycerol synthesis; Transgenic algae; Intermittent heat shock. dc.title. ... Effect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas Reinhardtii. dc. ... Effect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas Reinhardtii. en_ ... optimized according to the codon bias of Chlamydomonas reinhardtii, were inserted into the genomic DNA of model microalga C. ...
https://openrepository.aut.ac.nz/handle/10292/13458?show=full
EMDB < EMD...EMDB < EMD...
Monomeric PSI of Chlamydomonas reinhardtii at 2.31 A resolution ... Monomeric PSI of Chlamydomonas reinhardtii at 2.31 A resolution ... Sample Organism: Chlamydomonas reinhardtii Sample: Photosystem I dimer of Chlamydomonas reinhardtii. Fitted models: 7zqc () ...
https://www.ebi.ac.uk/emdb/EMD-14870
Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii<...Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii<...
Dutcher, Susan K. / Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii. In: ... Dutcher, S. K. (1995). Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii. Methods ... Dutcher, SK 1995, Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii, Methods in ... Chapter 45 Purification of Basal Bodies and Basal Body Complexes from Chlamydomonas reinhardtii. Methods in cell biology. 1995 ...
https://profiles.wustl.edu/en/publications/chapter-45-purification-of-basal-bodies-and-basal-body-complexes-
TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtiiTIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii
Chlamydomonas reinhardtii. Insertion mutation. Sequence motif analysis. Mutagenesis. DNA. Polymerase chain reaction. Algae. ... TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii. PLoS One. 2020 May 13;15(5): ... TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii. ... CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas reinhardtii. TIM utilizes delivery ...
https://repository.escholarship.umassmed.edu/handle/20.500.14038/48441
Chlamydomonas reinhardtii encodes a single sigma70-like factor which likely functions in chloroplast transcription
Chlamydomonas reinhardtii EST clones encoding a protein highly similar to prokaryotic sigma factors and plant sigma-like ... Chlamydomonas reinhardtii EST clones encoding a protein highly similar to prokaryotic sigma factors and plant sigma-like ... The Protein Disulfide Isomerase-like RB60 Is Partitioned between Stroma and Thylakoids in Chlamydomonas reinhardtii ... Differential regulation of chloroplast gene expression in Chlamydomonas reinhardtii during photoacclimation: Light stress ...
https://volcaniarchive.agri.gov.il/skn/e18737?nsid=2
Direct lipid extraction from wet Chlamydomonas reinhardtii biomass using osmotic shock - Fingerprint - Korea University
Direct lipid extraction from wet Chlamydomonas reinhardtii biomass using osmotic shock. Gursong Yoo, Won Kun Park, Chul Woong ... Dive into the research topics of Direct lipid extraction from wet Chlamydomonas reinhardtii biomass using osmotic shock. ...
https://koreauniv.pure.elsevier.com/en/publications/direct-lipid-extraction-from-wet-chlamydomonas-reinhardtii-biomas/fingerprints/
petD mRNA maturation in Chlamydomonas reinhardtii chloroplasts: Role of 5 endonucleolytic processing - Boyce Thompson InstitutepetD mRNA maturation in Chlamydomonas reinhardtii chloroplasts: Role of 5' endonucleolytic processing - Boyce Thompson Institute
petD mRNA maturation in Chlamydomonas reinhardtii chloroplasts: Role of 5′ endonucleolytic processing. by Mike Rezl , Feb 18, ...
https://btiscience.org/publication/petd-mrna-maturation-in-chlamydomonas-reinhardtii-chloroplasts-role-of-5-endonucleolytic-processing/
Translatomics and physiological analyses of the detoxification mechanism of green alga Chlamydomonas reinhardtii to cadmium...Translatomics and physiological analyses of the detoxification mechanism of green alga Chlamydomonas reinhardtii to cadmium...
Cadmio; Chlamydomonas reinhardtii; Transportadoras de Casetes de Unión a ATP; Cadmio/toxicidad; Chlamydomonas reinhardtii/ ... Chlamydomonas reinhardtii Idioma: Inglés Revista: J Hazard Mater Asunto de la revista: Salud Ambiental Año: 2023 Tipo del ... Chlamydomonas reinhardtii Idioma: Inglés Revista: J Hazard Mater Asunto de la revista: Salud Ambiental Año: 2023 Tipo del ... Here, we analyzed the translatome of the green alga Chlamydomonas reinhardtii following treatment with Cd to identify the ...
https://pesquisa.bvsalud.org/bolivia/resource/es/mdl-36860060
IMSEAR at SEARO: Local repeat sequence organization of an intergenic spacer in the chloroplast genome of Chlamydomonas...
Local repeat sequence organization of an intergenic spacer in the chloroplast genome of Chlamydomonas reinhardtii leads to DNA ... Local repeat sequence organization of an intergenic spacer in the chloroplast genome of Chlamydomonas reinhardtii leads to DNA ... in a cross between a pair of polymorphic interfertile strains of Chlamydomonas (C. reinhardtii and C. minnesotti), suggesting ... markers were obtained from total genomic DNA of Chlamydomonas reinhardtii. Such parent-specific RAPD bands (genomic ...
https://imsear.searo.who.int/handle/123456789/111152
Towards an understanding of channelrhodopsin function: simulations lead to novel insights of the channel mechanismTowards an understanding of channelrhodopsin function: simulations lead to novel insights of the channel mechanism
Chlamydomonas reinhardtii / chemistry* * Chlamydomonas reinhardtii / genetics * Chlamydomonas reinhardtii / physiology * ...
https://pubmed.ncbi.nlm.nih.gov/23376098/
RCSB PDB - 1AF5: GROUP I MOBILE INTRON ENDONUCLEASERCSB PDB - 1AF5: GROUP I MOBILE INTRON ENDONUCLEASE
Chlamydomonas reinhardtii. Mutation(s): 1 EC: 3.1. UniProt. Find proteins for P05725 (Chlamydomonas reinhardtii) ...
https://www.rcsb.org/structure/1af5
Genes search | AnalogYeastGenes search | AnalogYeast
Chlamydomonas reinhardtii 4uzy_a A8ITN7 99.80 7.30E-24 4.00E-28 234.10 0 0 0 0 0 0 0 0 ...
https://www.weizmann.ac.il/molgen/AnalogYeast/genes-search?search=YDR416W
View of Single-cell MALDI Tandem Mass Spectrometry: Unambiguous Assignment of Small Biomolecules from Single Chlamydomonas...View of Single-cell MALDI Tandem Mass Spectrometry: Unambiguous Assignment of Small Biomolecules from Single Chlamydomonas...
Unambiguous Assignment of Small Biomolecules from Single Chlamydomonas reinhardtii Cells Download Download PDF ...
https://ojs.chimia.ch/chimia/article/view/2016_236/1065
BioenergeticsBioenergetics
Here we show that in the green alga Chlamydomonas reinhardtii, LHCSR3 modulates the excitation energy flow and dissipates the ... In the green alga Chlamydomonas reinhardtii, non-photochemical quenching becomes activated upon high light acclimation and ... in the model green alga Chlamydomonas reinhardtii. Activation of NPQ requires low pH in the thylakoid lumen, which is induced ... II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. ...
https://www.jbc.org/bioenergetics?startPage=&KeywordRaw=photosystem%20II
IFT140 gene: MedlinePlus GeneticsIFT140 gene: MedlinePlus Genetics
Subunit interactions and organization of the Chlamydomonas reinhardtii intraflagellar transport complex A proteins. J Biol Chem ...
https://medlineplus.gov/genetics/gene/ift140/
Guanine | C5H5N5O | ChemSpiderGuanine | C5H5N5O | ChemSpider
Chlamydomonas reinhardtii PlantCyc GUANINE. Chromochloris zofingiensis PlantCyc GUANINE. Cicer arietinum PlantCyc GUANINE. ...
http://www.chemspider.com/Chemical-Structure.744.html
Addgene: pAAV-GFAP104-ChR2-mCherryAddgene: pAAV-GFAP104-ChR2-mCherry
Chlamydomonas reinhardtii * Insert Size (bp). 1650 * GenBank ID. EU714030.1 * Promoter GFAP104 * Tag / Fusion Protein *mCherry ...
https://www.addgene.org/58892/
Microalga Chlamydomonas reinhardtiiAlga Chlamydomonas reinhardtiiSpeciesGenomeChloroplastAlgaeIntraflagellar transportVegetativeProteinsOrganismLipidDangGlobosaMotilityGreenProteinPhotosynthesisStrainsGenomicCarbonGeneMethodsProtocolsSimilarPair
Microalga Chlamydomonas reinhardtii2
  • Herein we provide the first molecular and enzymatic characterization of the ADHE from the photosynthetic microalga Chlamydomonas reinhardtii. (archives-ouvertes.fr)
  • Cobamide remodeling in the freshwater microalga Chlamydomonas reinhardtii. (mpg.de)
Alga Chlamydomonas reinhardtii7
  • Translatomics and physiological analyses of the detoxification mechanism of green alga Chlamydomonas reinhardtii to cadmium toxicity. (bvsalud.org)
  • Here, we analyzed the translatome of the green alga Chlamydomonas reinhardtii following treatment with Cd to identify the cellular and physiological responses to Cd stress. (bvsalud.org)
  • Subsequently, we apply our method to the recently sequenced green alga Chlamydomonas reinhardtii . (rsc.org)
  • Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii. (wjgnet.com)
  • Nuclear transformation in the model alga Chlamydomonas reinhardtii. (mpg.de)
  • They have confirmed for the first time that a plant, the green alga Chlamydomonas reinhardtii, not only engages in photosynthesis, but also has an alternative source of energy: it can draw it from other plants. (uni-bielefeld.de)
  • The alga Chlamydomonas reinhardtii is a single-cell organism. (uni-bielefeld.de)
Species6
  • Since Chlamydomonas species are normally haploid, the effects of mutations are seen immediately without further crosses. (bionity.com)
  • Vegetative cells of the reinhardtii species are haploid with 17 small chromosomes. (bionity.com)
  • The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas orbicularis E. G. Pringsh. (elsevier.com)
  • C. reinhardtii was most closely related to C. globosa, and they were shown to be different species. (elsevier.com)
  • Discovery of the Japanese strains of C. reinhardtii supports the cosmopolitan distribution of this species. (elsevier.com)
  • In a series of experiments, Professor Dr. Olaf Kruse and his team cultivated the microscopically small green alga species Chlamydomonas reinhardtii in a low carbon dioxide environment and observed that when faced with such a shortage, these single-cell plants can draw energy from neighbouring vegetable cellulose instead. (uni-bielefeld.de)
Genome6
  • In 2007, the complete nuclear genome sequence of C. reinhardtii was published. (bionity.com)
  • The current draft (Chlre3) of the Chlamydomonas nuclear genome sequence prepared by Joint Genome Institute of the U.S. Dept of Energy comprises 1557 scaffolds totaling 120 Mb. (bionity.com)
  • Such parent-specific RAPD bands (genomic fingerprints) segregated uniparentally (through mt+) in a cross between a pair of polymorphic interfertile strains of Chlamydomonas (C. reinhardtii and C. minnesotti), suggesting that they originated from the chloroplast genome. (who.int)
  • We suggest specific genes in the genome of Chlamydomonas which are the strongest candidates for coding the responsible enzymes. (rsc.org)
  • The Chlamydomonas Genome Project, version 6: reference assemblies for mating type plus and minus strains reveal extensive structural mutation in the laboratory. (wjgnet.com)
  • He also has extensively studied the blue-green algae Chlamydomonas genome and is establishing methods for examining the set of RNA molecules and the function of proteins involved in their photosynthesis and acclimation. (carnegiescience.edu)
Chloroplast1
  • Ma K , Deng L, Wu H, Fan J. Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast. (wjgnet.com)
Algae3
  • The cilia on the algae Chlamydomonas reinhardtii look like two antennae on an ant's head. (kumc.edu)
  • Algae Chlamydomonas spp. (harvard.edu)
  • Using energy from sunlight and electrons from water, both the green algae Chlamydomonas reinhardtii and the cyanobacterium Synechocystis sp. (nrel.gov)
Intraflagellar transport1
  • Subunit interactions and organization of the Chlamydomonas reinhardtii intraflagellar transport complex A proteins. (medlineplus.gov)
Vegetative1
Proteins1
  • Biochemical analysis of the 17 proteins found in the Chlamydomonas IFT particles has begun toreveal their complex oligomeric organization. (uidaho.edu)
Organism2
  • A primary function of IFT is totransport axonemal building blocks out to the distal tip which serves as the site of assembly for the organelle.The model organism for the study of IFT is the unicellular biflagellate green alga, Chlamydomonasreinhardtii. (uidaho.edu)
  • Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas reinhardtii. (umassmed.edu)
Lipid2
  • To enhance lipid content, both lysophosphatidic acyltransferase gene (c-lpaat) and glycerol-3-phosphate dehydrogenase gene (c-gpd1), optimized according to the codon bias of Chlamydomonas reinhardtii, were inserted into the genomic DNA of model microalga C. reinhardtii by the glass bead method. (aut.ac.nz)
  • The results of this study offer a new strategy combining genetic manipulation and intermittent heat shock to enhance lipid production, especially the production of long-chain saturated fatty acids, using C. reinhardtii. (aut.ac.nz)
Dang1
Globosa2
  • In this study, new strains of C. reinhardtii and C. globosa were isolated from Japan and compared with several strains similar to C. reinhardtii. (elsevier.com)
  • Based on Japanese strains and/or strains from other countries, emended descriptions of C. reinhardtii, C. globosa, and C. orbicularis are given. (elsevier.com)
Motility1
  • Modeling of Chlamydomonas reinhardtii motility in confinement. (mpg.de)
Green2
  • Chlamydomonas reinhardtii is a motile single celled green alga about 10 micrometres in diameter that swims with two flagella . (bionity.com)
  • An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas. (mpg.de)
Protein1
  • In this study, we showed that CrPTC1, encoding a protein with both SPX and SLC (permease solute carrier 13) domains for Pi transport, and CrVTC4, encoding a protein with both SPX and vacuolar transporter chaperone (VTC) domains for polyP synthesis, are required for vacuolar polyP accumulation in the chlorophyte Chlamydomonas reinhardtii. (archives-ouvertes.fr)
Photosynthesis2
  • Normal Chlamydomonas can grow on a simple medium of inorganic salts in the light, using photosynthesis to provide energy. (bionity.com)
  • It was Chlamydomonas reinhardtii , a one-celled alga often used in research to study many cell behaviors such as photosynthesis, biofuel production and the evolution of single-celled organisms into multicellular ones. (kumc.edu)
Strains1
  • Based on the morphological, genealogical, phylogenetical, and mating studies including the new Japanese strains, the circumscription of C. reinhardtii was clarified. (elsevier.com)
Genomic1
  • Parent-specific, randomly amplified polymorphic DNA (RAPD) markers were obtained from total genomic DNA of Chlamydomonas reinhardtii. (who.int)
Carbon1
  • Chlamydomonas reinhardtii, ac-208, is an acetate-requiring mutant which was isolated on the basis of its inability to fix carbon dioxide and is derived from the wild type (137c) by ultra violet irradiation. (caltech.edu)
Gene1
  • Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Ex" by Wei Fang, Yaqing Si et al. (unl.edu)
Methods1
  • TIM achieves a far higher mutation rate than any previously reported for CRISPR-based methods in C. reinhardtii and promises to be effective for many, if not all, non-essential nuclear genes. (umassmed.edu)
Protocols1
  • In Chlamydomonas, three different protocols can be used to obtain isolated basal body complexes. (wustl.edu)
Similar2
  • Although C. reinhardtii was similar to C. orbicularis, the authentic strain of C. orbicularis was morphologically distinguishable and phylogenetically distant from C. reinhardtii. (elsevier.com)
  • VChR2 is similar to the ChR2 counterpart from Chlamydomonas reinhardtii with respect to its absorption maximum ( 460 nm) and photocycle dynamics. (uni-bielefeld.de)
Pair1
  • The microtubule organizing center (MTOC) in Chlamydomonas is defined as the pair of basal bodies, the rootlet microtubules, the distal and proximal striated fibers, and the nucleobasal body connectors (NBBCs). (wustl.edu)
How does eukaryotic gene prediction work? | Nature Biotechnology
How does eukaryotic gene prediction work? | Nature Biotechnology (nature.com)
EMDB < EMD...
EMDB < EMD... (ebi.ac.uk)
RCSB PDB - 1AF5: GROUP I MOBILE INTRON ENDONUCLEASE
RCSB PDB - 1AF5: GROUP I MOBILE INTRON ENDONUCLEASE (rcsb.org)
Genes search | AnalogYeast
Genes search | AnalogYeast (weizmann.ac.il)
Recently Published GRAS Notices and FDA Letters | FDA
Recently Published GRAS Notices and FDA Letters | FDA (fda.gov)
PDF) Biotechnological strategies for the recovery of valuable and critical raw materials from waste electrical and electronic...
PDF) Biotechnological strategies for the recovery of valuable and critical raw materials from waste electrical and electronic... (researchgate.net)
Fundamental shift in vitamin B12 eco-physiology of a model alga demonstrated by experimental evolution | The ISME Journal
Fundamental shift in vitamin B12 eco-physiology of a model alga demonstrated by experimental evolution | The ISME Journal (nature.com)
IFT140 gene: MedlinePlus Genetics
IFT140 gene: MedlinePlus Genetics (medlineplus.gov)
Prof. Dr. Katrin Philippar | Naturwissenschaftlich-Technische Fakultät | Universität des Saarlandes
Prof. Dr. Katrin Philippar | Naturwissenschaftlich-Technische Fakultät | Universität des Saarlandes (uni-saarland.de)
Uracil | C4H4N2O2 | ChemSpider
Uracil | C4H4N2O2 | ChemSpider (chemspider.com)
Addgene: pAAV-GFAP104-ChR2-mCherry
Addgene: pAAV-GFAP104-ChR2-mCherry (addgene.org)
Volume 66 Issue 3 | Avian Diseases
Volume 66 Issue 3 | Avian Diseases (bioone.org)
Zooming in on the phycosphere: the ecological interface for phytoplankton-bacteria relationships | Nature Microbiology
Zooming in on the phycosphere: the ecological interface for phytoplankton-bacteria relationships | Nature Microbiology (nature.com)
Vauthey Research Group - Group Members
Vauthey Research Group - Group Members (unige.ch)
Sequence search - BRENDA Enzyme Database
Sequence search - BRENDA Enzyme Database (brenda-enzymes.org)
Species Summary in GenScript GenPool
Species Summary in GenScript GenPool (genscript.com)
Photosynthesis: Oxygen overload | eLife
Photosynthesis: Oxygen overload | eLife (elifesciences.org)
SGN Unigene - Show All Stored BLAST Hits - Sol Genomics Network
SGN Unigene - Show All Stored BLAST Hits - Sol Genomics Network (sgn.cornell.edu)
Figures and data in The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas...
Figures and data in The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas... (elifesciences.org)
Lichtwahrnehmung und Lichtnutzung
Lichtwahrnehmung und Lichtnutzung (kersti.de)
Horseradish flea beetle: Protected with the weapons of its food plant | Max Planck Institute for Chemical Ecology
Horseradish flea beetle: Protected with the weapons of its food plant | Max Planck Institute for Chemical Ecology (ice.mpg.de)
An integrative approach towards completing genome-scale metabolic networks - Molecular BioSystems (RSC Publishing)
An integrative approach towards completing genome-scale metabolic networks - Molecular BioSystems (RSC Publishing) (pubs.rsc.org)
World Journal of Gastroenterology - Baishideng Publishing Group
World Journal of Gastroenterology - Baishideng Publishing Group (wjgnet.com)
Diya'uddeen HASAN | PhD | University of Malaya, Kuala Lumpur | UM | Department of Chemical Engineering
Diya'uddeen HASAN | PhD | University of Malaya, Kuala Lumpur | UM | Department of Chemical Engineering (researchgate.net)
Home - Prof Klaus Hellgardt
Home - Prof Klaus Hellgardt (imperial.ac.uk)
Life and Death in a Drop of Water - Reed Magazine - Reed College
Life and Death in a Drop of Water - Reed Magazine - Reed College (reed.edu)
Publikationen von Reimo Zoschke | Max-Planck-Institut für Molekulare Pflanzenphysiologie
Publikationen von Reimo Zoschke | Max-Planck-Institut für Molekulare Pflanzenphysiologie (mpimp-golm.mpg.de)
University of Edinburgh - Datasets - University of Edinburgh Research Explorer
University of Edinburgh - Datasets - University of Edinburgh Research Explorer (research.ed.ac.uk)
SCOPe 2.08: Domain d1q90c_: 1q90 C
SCOPe 2.08: Domain d1q90c_: 1q90 C (scop.berkeley.edu)
Publikationen von Karin Köhl | Max-Planck-Institut für Molekulare Pflanzenphysiologie
Publikationen von Karin Köhl | Max-Planck-Institut für Molekulare Pflanzenphysiologie (mpimp-golm.mpg.de)