A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.
A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.
Proteins found in any species of algae.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
Ribonucleic acid in algae having regulatory and catalytic roles as well as involvement in protein synthesis.
A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.
A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.
A bundle of MICROTUBULES and MICROTUBULE-ASSOCIATED PROTEINS forming the core of each CILIUM or FLAGELLUM. In most eukaryotic cilia or flagella, an axoneme shaft has 20 microtubules arranged in nine doublets and two singlets.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Cytochromes f are found as components of the CYTOCHROME B6F COMPLEX. They play important role in the transfer of electrons from PHOTOSYSTEM I to PHOTOSYSTEM II.
Deoxyribonucleic acid that makes up the genetic material of algae.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
A protein complex that includes CYTOCHROME B6 and CYTOCHROME F. It is found in the THYLAKOID MEMBRANE and plays an important role in process of PHOTOSYNTHESIS by transferring electrons from PLASTOQUINONE to PLASTOCYANIN or CYTOCHROME C6. The transfer of electrons is coupled to the transport of PROTONS across the membrane.
A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Proteins found in any species of protozoan.
The functional hereditary units of protozoa.
A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Protein complexes that take part in the process of PHOTOSYNTHESIS. They are located within the THYLAKOID MEMBRANES of plant CHLOROPLASTS and a variety of structures in more primitive organisms. There are two major complexes involved in the photosynthetic process called PHOTOSYSTEM I and PHOTOSYSTEM II.
A copper-containing plant protein that is a fundamental link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
Membranous cisternae of the CHLOROPLAST containing photosynthetic pigments, reaction centers, and the electron-transport chain. Each thylakoid consists of a flattened sac of membrane enclosing a narrow intra-thylakoid space (Lackie and Dow, Dictionary of Cell Biology, 2nd ed). Individual thylakoids are interconnected and tend to stack to form aggregates called grana. They are found in cyanobacteria and all plants.
Complexes containing CHLOROPHYLL and other photosensitive molecules. They serve to capture energy in the form of PHOTONS and are generally found as components of the PHOTOSYSTEM I PROTEIN COMPLEX or the PHOTOSYSTEM II PROTEIN COMPLEX.
A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
A genus of GREEN ALGAE in the family Volvocaceae. They form spherical colonies of hundreds or thousands of bi-flagellated cells in a semi-transparent gelatinous ball.
Polyunsaturated side-chain quinone derivative which is an important link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A pre-emergent herbicide.
The absence of light.
A highly branched glucan in starch.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Ribonucleic acid in chloroplasts having regulatory and catalytic roles as well as involvement in protein synthesis.
Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Cytochromes of the c type that are involved in the transfer of electrons from CYTOCHROME B6F COMPLEX and PHOTOSYSTEM I.
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins encoded by the CHLOROPLAST GENOME or proteins encoded by the nuclear genome that are imported to and resident in the CHOROPLASTS.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Cytochromes (electron-transporting proteins) with protoheme (HEME B) as the prosthetic group.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
Self-replicating, short, fibrous, rod-shaped organelles. Each centriole is a short cylinder containing nine pairs of peripheral microtubules, arranged so as to form the wall of the cylinder.
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
The relationships of groups of organisms as reflected by their genetic makeup.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The sum of the weight of all the atoms in a molecule.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
The functional hereditary units of PLANTS.
A family of zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide. They play an important role in the transport of CARBON DIOXIDE from the tissues to the LUNG. EC 4.2.1.1.
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
An enzyme that catalyzes the transfer of glucose from ADPglucose to glucose-containing polysaccharides in 1,4-alpha-linkages. EC 2.4.1.21.
The rate dynamics in chemical or physical systems.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Proteins found in the microtubules.
Nonmotile unicellular green algae potentially valuable as a source of high-grade protein and B-complex vitamins.
An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of gonorrhea.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A potassium salt used to replenish ELECTROLYTES, for restoration of WATER-ELECTROLYTE BALANCE, as well as a urinary and systemic alkalizer, which can be administered orally or by intravenous infusion. Formerly, it was used in DIURETICS and EXPECTORANTS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The branch of biology dealing with the effect of light on organisms.
Processes by which phototrophic organisms use sunlight as their primary energy source. Contrasts with chemotrophic processes which do not depend on light and function in deriving energy from exogenous chemical sources. Photoautotrophy (or photolithotrophy) is the ability to use sunlight as energy to fix inorganic nutrients to be used for other organic requirements. Photoautotrophs include all GREEN PLANTS; GREEN ALGAE; CYANOBACTERIA; and green and PURPLE SULFUR BACTERIA. Photoheterotrophs or photoorganotrophs require a supply of organic nutrients for their organic requirements but use sunlight as their primary energy source; examples include certain PURPLE NONSULFUR BACTERIA. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; HETEROTROPHY; chemotrophy; or phototrophy) to utilize different sources to meet their nutrients and energy requirements.
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.
Dyneins that are responsible for ciliary and flagellar beating.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An order of CHLOROPHYTA commonly found in freshwater habitats. Characteristics include the presence of a cellulose wall and two to four equal, smooth, apical flagella.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.
An autosomal recessive disorder characterized by a triad of DEXTROCARDIA; INFERTILITY; and SINUSITIS. The syndrome is caused by mutations of DYNEIN genes encoding motility proteins which are components of sperm tails, and CILIA in the respiratory and the reproductive tracts.
Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
An enzyme that catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX by the conversion of two propionate groups to two vinyl groups. It is the sixth enzyme in the 8-enzyme biosynthetic pathway of HEME, and is encoded by CPO gene. Mutations of CPO gene result in HEREDITARY COPROPORPHYRIA.
The quantity of volume or surface area of ORGANELLES.

The internal Cys-207 of sorghum leaf NADP-malate dehydrogenase can form mixed disulphides with thioredoxin. (1/808)

The role of the internal Cys-207 of sorghum NADP-malate dehydrogenase (NADP-MDH) in the activation of the enzyme has been investigated through the examination of the ability of this residue to form mixed disulphides with thioredoxin mutated at either of its two active-site cysteines. The h-type Chlamydomonas thioredoxin was used, because it has no additional cysteines in the primary sequence besides the active-site cysteines. Both thioredoxin mutants proved equally efficient in forming mixed disulphides with an NADP-MDH devoid of its N-terminal bridge either by truncation, or by mutation of its N-terminal cysteines. They were poorly efficient with the more compact WT oxidised NADP-MDH. Upon mutation of Cys-207, no mixed disulphide could be formed, showing that this cysteine is the only one, among the four internal cysteines, which can form mixed disulphides with thioredoxin. These experiments confirm that the opening of the N-terminal disulphide loosens the interaction between subunits, making Cys-207, located at the dimer contact area, more accessible.  (+info)

Chlamydomonas chloroplast ferrous hemoglobin. Heme pocket structure and reactions with ligands. (2/808)

We report the optical and resonance Raman spectral characterization of ferrous recombinant Chlamydomonas LI637 hemoglobin. We show that it is present in three pH-dependent equilibrium forms including a 4-coordinate species at acid pH, a 5-coordinate high spin species at neutral pH, and a 6-coordinate low spin species at alkaline pH. The proximal ligand to the heme is the imidazole group of a histidine. Kinetics of the reactions with ligands were determined by stopped-flow spectroscopy. At alkaline pH, combination with oxygen, nitric oxide, and carbon monoxide displays a kinetic behavior that is interpreted as being rate-limited by conversion of the 6-coordinate form to a reactive 5-coordinate form. At neutral pH, combination rates of the 5-coordinate form with oxygen and carbon monoxide were much faster (>10(7) microM-1 s-1). The dissociation rate constant measured for oxygen is among the slowest known, 0.014 s-1, and is independent of pH. Replacement of the tyrosine 63 (B10) by leucine or of the putative distal glutamine by glycine increases the dissociation rate constant 70- and 30-fold and increases the rate of autoxidation 20- and 90-fold, respectively. These results are consistent with at least two hydrogen bonds stabilizing the bound oxygen molecule, one from tyrosine B10 and the other from the distal glutamine. In addition, the high frequency (232 cm-1) of the iron-histidine bond suggests a structure that lacks any proximal strain thus contributing to high ligand affinity.  (+info)

Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas. (3/808)

A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR protocol, we recovered two cDhc sequences distinct from the axonemal Dhc sequences identified previously. cDhc1a is closely related to the major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor cDhc isoform identified in sea urchins, Caenorhabditis elegans, and Tetrahymena. The Chlamydomonas cDhc1b transcript is a low-abundance mRNA whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and identified two strains in which portions of the cDhc1b gene have been deleted. The two mutants assemble short flagellar stumps (<1-2 micrometer) filled with aberrant microtubules, raft-like particles, and other amorphous material. The results indicate that cDhc1b is involved in the transport of components required for flagellar assembly in Chlamydomonas.  (+info)

Cell division: The renaissance of the centriole. (4/808)

Centrioles are located at the center of the cytoskeleton and duplicate exactly once per cell cycle. Recent studies suggest that centrioles are required for the organization of a functional centrosome and that centriole assembly requires both gamma- and delta-tubulin.  (+info)

Intracellular motility: A special delivery service. (5/808)

Recent studies have identified a delivery service that operates in specialised cell appendages: two motor proteins and a novel protein organelle use axonemal microtubules as tracks to shuttle essential components to the tips of flagella and the dendrites of sensory neurons.  (+info)

Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. (6/808)

Eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. To identify new genes involved in the regulation of axonal transport in Drosophila melanogaster, we undertook a screen based upon the sluggish larval phenotype of known motor mutants. One of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis. These defects include intra-axonal accumulations of cargoes, severe axonal degeneration, and aberrant chromosome segregation. The gene identified by robl encodes a 97-amino acid polypeptide that is 57% identical (70% similar) to the 105-amino acid Chlamydomonas outer arm dynein-associated protein LC7, also reported here. Both robl and LC7 have homology to several other genes from fruit fly, nematode, and mammals, but not Saccharomyces cerevisiae. Furthermore, we demonstrate that members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. We propose that roadblock/LC7 family members may modulate specific dynein functions.  (+info)

Domains in the 1alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas. (7/808)

Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1alpha Dhc of the I1 inner dynein arm. The 1alpha Dhc is similar to other axonemal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH(2)- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1alpha Dhcs were recovered. The truncated 1alpha Dhc fragments lacked the dynein motor domain, but still assembled with the 1beta Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1alpha motor domain in the I1 structure and further revealed the role of the 1alpha Dhc in flagellar motility and phototactic behavior.  (+info)

The complete mitochondrial DNA sequences of Nephroselmis olivacea and Pedinomonas minor. Two radically different evolutionary patterns within green algae. (8/808)

Green plants appear to comprise two sister lineages, Chlorophyta (classes Chlorophyceae, Ulvophyceae, Trebouxiophyceae, and Prasinophyceae) and Streptophyta (Charophyceae and Embryophyta, or land plants). To gain insight into the nature of the ancestral green plant mitochondrial genome, we have sequenced the mitochondrial DNAs (mtDNAs) of Nephroselmis olivacea and Pedinomonas minor. These two green algae are presumptive members of the Prasinophyceae. This class is thought to include descendants of the earliest diverging green algae. We find that Nephroselmis and Pedinomonas mtDNAs differ markedly in size, gene content, and gene organization. Of the green algal mtDNAs sequenced so far, that of Nephroselmis (45,223 bp) is the most ancestral (minimally diverged) and occupies the phylogenetically most basal position within the Chlorophyta. Its repertoire of 69 genes closely resembles that in the mtDNA of Prototheca wickerhamii, a later diverging trebouxiophycean green alga. Three of the Nephroselmis genes (nad10, rpl14, and rnpB) have not been identified in previously sequenced mtDNAs of green algae and land plants. In contrast, the 25,137-bp Pedinomonas mtDNA contains only 22 genes and retains few recognizably ancestral features. In several respects, including gene content and rate of sequence divergence, Pedinomonas mtDNA resembles the reduced mtDNAs of chlamydomonad algae, with which it is robustly affiliated in phylogenetic analyses. Our results confirm the existence of two radically different patterns of mitochondrial genome evolution within the green algae.  (+info)

Assembly of flagellar radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3 ...
Previous physiological and pharmacological experiments have demonstrated that the Chlamydomonas flagellar axoneme contains a cAMP-dependent protein kinase (PKA) that regulates axonemal motility and dynein activity. However, the mechanism for anchoring PKA in the axoneme is unknown. Here we test the hypothesis that the axoneme contains an A-kinase anchoring protein (AKAP). By performing RII blot overlays on motility mutants defective for specific axonemal structures, two axonemal AKAPs have been identified: a 240-kD AKAP associated with the central pair apparatus, and a 97-kD AKAP located in the radial spoke stalk. Based on a detailed analysis, we have shown that AKAP97 is radial spoke protein 3 (RSP3). By expressing truncated forms of RSP3, we have localized the RII-binding domain to a region between amino acids 144-180. Amino acids 161-180 are homologous with the RII-binding domains of other AKAPs and are predicted to form an amphipathic helix. Amino acid substitution of the central residues of ...
With 1 Figure in the Text. This work was conducted in the Department of Biological Sciences at Stanford University, and was supported in part by a Henry Newell Fellowship, and in part by a Research Fellowship (GF-6012) from the National Institutes of Health. ...
The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions - a spoke head, a bifurcated neck and a stalk. While the neck and the stalk are asymmetric, two-fold symmetry of the head has remained controversial.. In this work we used single particle cryo-EM analysis to generate 3D structure of the whole radial spoke at unprecedented resolution. We show the head region at 15Å resolution and confirm two-fold symmetry. Using distance constraints generated by crosslinking mass spectrometry we locate two components, RSP2 and 4 at the head/neck regions. Our biophysical analysis of isolated RSPs 4, 9, and 10 affirmed their oligomeric state. Our results enabled us to redefine the boundaries of the regions and propose a model of organization of the radial spoke component proteins. ...
We have shown that the Chlamydomonas mutants ida5 (Kato et al., 1993) and ida5-t, lacking four subspecies of inner-arm dynein, have mutations in an actin-encoding gene such that its gene product is totally lost. The axoneme of these mutants contain NAP instead of conventional actin, as evidenced by the two novel spots in two-dimensional electrophoresis patterns. These findings are unexpected since Chlamydomonas and a related species, Volvox, have been shown to have a single gene for actin, which is ∼90% homologous to rabbit skeletal muscle actin (Cresnar et al., 1990; Sugase et al., 1996). The fact that ida5 and ida5-t grow normally suggests either that NAP or other proteins may be able to substitute for conventional actin in important functions, or that actin is not essential for the growth of Chlamydomonas.. The molecular identity of NAP remains to be studied, but it appears to be somehow related to actin. This is because NAP and actin have similar molecular weights, can serve as the subunit ...
Amoroso, G., D. Sueltemeyer, C. Thyssen and H.P. Fock (1998). Uptake of HCO3- and CO2 in cells and chloroplasts from the microalgae Chlamydomonas reinhardtii and Dunaliella tertiolecta. Plant Physiol. 116, 193-201. Asleson, C.M. and P.A. Lefebvre (1998). Genetic analysis of flagellar length control in Chlamydomonas reinhardtii: A new long-flagella locus and extragenic suppressor mutations. Genetics 148, 693-702. Bhattacharya, D. and L. Medlin (1998). Algal phylogeny and the origin of land plants. Plant Physiol. 116, 9-15. Boschetti, A. and K. Schmid (1998). Energy supply for ATP-synthase deficient chloroplasts of Chlamydomonas reinhardii. Plant Cell Physiol. 39, 160-168. Brosch-Salomon, S., M. Hoeftberger, A. Holzinger and U. Luetz-Meindl (1998). Ultrastructural localization of polysaccharides and N-acetyl-D-galactosamine in the secretory pathway of green algae (Desmidiaceae). J. Exp. Bot. 49, 145-153. Calenberg, M., U. Brohsonn, M. Zedlacher and G. Kreimer (1998). Light- and Ca2+-modulated ...
Fingerprint Dive into the research topics of Role of timer and sizer in regulation of Chlamydomonas cell cycle. Together they form a unique fingerprint. ...
Molecular analysis of LC7 from the Chlamydomonas outer dynein arm. (A) Two tryptic peptides from outer arm dynein LC7 were completely sequenced, yielding a tota
Fertilization tubules in wild-type (A) and ida5 (B) mt+ gametes produced in response to a 1-h exposure to 10 mM dibutyryl-cAMP and 1 mM IBMX. Bar, 0.3 μm.
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Chlamydomonas monoica undergoes intraclonal mating-type differentiation (homothallism). Although the species differs in this regard from the more commonly studied heterothallic C. reinhardtii, cell-cell interactions and progression of the sexual cycle are similar for many homothallic and heterothallic species of the genus. Regulation of chloroplast gene transmission by the nuclear mating-type alleles (mt+ and mt-) is another common denominator for Chlamydomonas species studied thus far. We have previously reported the use of chloroplast inheritance patterns to identify mutants of C. monoica that have lost the potential to function as the mt+ mating-type. A similar screening procedure led to the isolation of an unusual mutant, mtl-3 whose phenotype is less readily explained. Chloroplast gene transmission patterns in crosses involving mtl-3 suggest that the mtl-3 strain mates preferentially as mt+. However, normal mating efficiencies and high zygospore viability are observed in clonal culture, ...
Read UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
In 1933, Marjory Stephenson and her student Stickland reported that cell suspensions catalysed the reduction of methylene blue with H2. Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce hydrogen.[17] In the late 1990s Anastasios Melis discovered that deprivation of sulfur induces the alga to switch from the production of oxygen (normal photosynthesis) to the production of hydrogen. He found that the enzyme responsible for this reaction is hydrogenase, but that the hydrogenase lost this function in the presence of oxygen. Melis also discovered that depleting the amount of sulfur available to the algae interrupted their internal oxygen flow, allowing the hydrogenase an environment in which it can react, causing the algae to produce hydrogen.[18] Chlamydomonas moewusii is also a promising strain for the production of hydrogen.[19][20] ...
Definition of chlamydomonas - a common single-celled green alga which typically has two flagella for swimming, living in water and moist soil.
Anti-Lhcb4 (CP29) Chlamydomonas reinhardti, Lhcb4, CP29, Lhcbm4 antibody , Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas, Q93WD2, AS06 117
Fingerprint Dive into the research topics of Nucleus-basal body connector in chlamydomonas: Evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Together they form a unique fingerprint. ...
Algae Detail UTEX Number: 943Class: ChlorophyceaeStrain: Chlamydomonas giganteaMedia: Modified Bold 3N MediumOrigin: Description of Location: Type Culture: NoCo
Algae Detail UTEX Number: 969Class: ChlorophyceaeStrain: Chlamydomonas applanataMedia: Modified Bold 3N MediumOrigin: Williamson Co., Texas, USADescription of L
Immunofluorescence micrograph of a Chlamydomonas cell stained with acetylated tubulin antibody to label flagella (green) and with an antibody against the protein EB1 that labels the flagella tip and base (red/yellow). Image from Lotte Pedersen, University of Copenhagen. To previous page ...
Introduction Bio 2010 01/31/2014 The objectives of this lab were to understand natural selection and understand the biotic and abiotic characteristics of a niche. In order to understand natural selection in this lab we observed characteristics of three different algae families; Volvox, Chlamydomonas, and Gonium. By observing these algae will give us a better understanding of how a simple cellular organism evolved due to natural selection and are able to survive today. These organisms will give us context into natural selection occurring on bigger organisms as we move on in lab. Similarly, in order to understand biotic and abiotic characteristics of a niche, we observed a transect. By observing a transect will provide insight into how the biosphere works by focussing on biotic and biotic components of a home to many organisms. Procedure 1: The Volvicine Line In this experiment we observed an isogamous, single celled, motile alga called chlamydomonas. We prepared a slide of a living Chlamydomonas ...
Figure 5A. Wildtype and roc15 mutant Chlamydomonas was entrained to a 12:12 light:dark cycle. The cultures were transferred to constant darkness and their tufA expression was measured with luminescence. They were then expressed to 5 minute long pulses of light. The wild type Chlamydomonas responded to the light pulses, evident by a phase shift in tufA expression. The roc15 mutant Chlamydomonas did not exhibit this phase shift. This indicate that roc15 is essential for circadian regulated phase shifts and entrainment. ...
View Notes - 10. [R] nlm = non linear minimization (Jan24) from AM 1234 at UWO. model.fit Page 1 of 1 25/01/2011 http://www.stats.uwo.ca/faculty/kulperger/Stat3858/Computing/RScripts/nlm-eg-exp.txt
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Alphabetic Listing of Presenting Authors - R. If you have any questions or comments then please send an email to [email protected] ...
pep:novel chromosome:VEGA66:10:33905485:33915883:1 gene:OTTMUSG00000029742 transcript:OTTMUST00000073807 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Rsph4a description:radial spoke head 4 homolog A (Chlamydomonas ...
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The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added ligand, THB1 coordinates the heme iron with the canonical proximal histidine and a distal lysine. In the cyanomet state, THB1 is structurally similar to other known truncated hemoglobins, particularly the heme domain of Chlamydomonas eugametos LI637, a light-induced chloroplastic hemoglobin. Recombinant THB1 is capable of binding nitric oxide (NO(*)) in either the ferric or ferrous state and has efficient NO(*) dioxygenase activity. By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is
RNA pyrophosphohydrolase (RppH) catalyzes the removal of pyrophosphate from 5 triphosphorylated RNAs thereby initiating RNA degradation. The enzyme has originally been identified in bacteria but homologs are present in eukaryotes where they are thought to be located in plastids or mitochondria. A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5rppH-GFP construct.. A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, sequencing, and DNA and RNA blotting techniques were used to indentify positive transformants at the DNA ...
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells ...
Phototaxis is one of the most fundamental stimulus-response behaviors in biology wherein motile microorganisms sense light gradients to swim toward the light source. Apart from single-cell survival and growth, it plays a major role at the global scale of aquatic ecosystems and bioreactors. We study phototaxis of single-celled algae Chlamydomonas reinhardtii as a function of cell number density and light stimulus using high spatiotemporal video microscopy. Surprisingly, the phototactic efficiency has a minimum at a well-defined number density, for a given light gradient, above which the phototaxis behavior of a collection of cells can even exceed the performance obtainable from single isolated cells. We show that the origin of enhancement of performance above the critical concentration lies in the slowing down of the cells, which enables them to sense light more effectively. We also show that this steady-state phenomenology is well captured by modeling the phototactic response as a ...
TY - JOUR. T1 - The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii. AU - Ozawa, Shin Ichiro. AU - Cavaiuolo, Marina. AU - Jarrige, Domitille. AU - Kuras, Richard. AU - Rutgers, Mark. AU - Eberhard, Stephan. AU - Drapier, Dominique. AU - Wollman, Francis André. AU - Choquet, Yves. PY - 2020/4/1. Y1 - 2020/4/1. N2 - In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. Here, we report the identification of the octotricopeptide repeat protein MTHI1, which is critical for the biogenesis of chloroplast ATP synthase oligomycin-sensitive chloroplast coupling factor. Unlike most trans-acting factors characterized so far in Chlamydomonas, which control the expression of a single gene, MTHI1 targets two distinct transcripts: it is required for the accumulation and translation of atpH mRNA, encoding a subunit of ...
During intraflagellar transport (IFT), the regulation of motor proteins, the loading and unloading of cargo and the turnover of flagellar proteins all occur at the flagellar tip. To begin an analysis of the protein composition of the flagellar tip, we used difference gel electrophoresis to compare long versus short (i.e., regenerating) flagella. The concentration of tip proteins should be higher relative to that of tubulin (which is constant per unit length of the flagellum) in short compared with long flagella. One protein we have identified is the cobalamin-independent form of methionine synthase (MetE). Antibodies to MetE label flagella in a punctate pattern reminiscent of IFT particle staining, and immunoblot analysis reveals that the amount of MetE in flagella is low in full-length flagella, increased in regenerating flagella, and highest in resorbing flagella. Four methylated proteins have been identified in resorbing flagella, using antibodies specific for asymmetrically dimethylated ...
We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.. ...
We report here on the UV-induced vanadate-dependent cleavage of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Both polypeptides are cleaved at a single site (termed the V1 site) by UV irradiation in the presence of Mg2+, ATP, and vanadate. The alpha chain yields fragments of Mr 290,000 and 190,000. Fragments of Mr 255,000 and 185,000 are obtained from the beta chain. Ultraviolet irradiation of the alpha and beta chains in the presence of vanadate and Mn2+ (but no nucleotide) induces cleavage of both molecules at sites (termed the V2 sites) distinct from the V1 sites. The single V2 site within the beta chain is located 75,000 daltons from the site of V1 cleavage within the Mr 255,000 V1 fragment. The alpha chain contains three distinct sites of V2 cleavage; all are located within the Mr 290,000 V1 fragment, 60,000, 90,000, and 100,000 daltons from the site of V1 cleavage. From these studies, we estimate the masses of the alpha and beta heavy chains to be 480,000 and
The Chlamydomonas Gopher Server at Duke University offers the following files: A description of the Chlamydomonas Genetics Center Catalogue of Chlamydomonas strains available from the Center List of plasmids available from the Center List of genes that have been sequenced from Chlamydomonas species also from Volvox and Dunaliella Recent literature citations Addresses of colleagues Nuclear genetic maps, in postscript and Macintosh bin-hex formats Chloroplast and mitochondrial genetic/physical maps, same formats Methods files This server can be accessed through the Duke University Computer ASSIST Center Gopher, who have kindly provided the following access information: Name=Duke University Computer ASSIST Center Type=1 Port=70 Path= Host=gopher.duke.edu If you are not running a gopher server, you can connect by pointing your gopher client to gopher.duke.edu, port 70. If you do not have access to a gopher client, gopher client and server software is available via anonymous ftp from ...
The radial spoke is known to play a role in the mechanical movement of the flagellum/cilium. For example, mutant organisms lacking properly functioning radial spokes have flagella and cilia that are immotile. Radial spokes also influence the cilium waveform; that is, the exact bending pattern the cilium repeats. How the radial spoke carries out this function is poorly understood. Radial spokes are believed to interact with both the central pair microtubules and the dynein arms, perhaps in a way that maintains the rhythmic activation of the dynein motors. For example, one of the radial spoke subunits, RSP3, is an anchor protein predicted to hold another protein called protein kinase A (PKA). PKA would theoretically then be able to activate/inactivate the adjacent dynein arms via its kinase activity. However, the identities and functions of the many radial spoke subunits are just beginning to be elucidated. ...
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis ...
The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. Current models of the CCM postulate that aseries of ion transporters bring HCO3- from outside the cell to thethylakoid lumen, where the carbonic anhydrase CAH3 dehydratesaccumulated HCO3- to CO2, raising the CO2 concentration forRubisco. Previously, HCO3- transporters have been identified atboth the plasma membrane and the chloroplast envelope, butthe transporter thought to be on the thylakoid membrane hasnot been identified. Three paralogous genes (BST1, BST2, BST3)belonging to the bestrophin family have been found to be upregulatedin low CO2 conditions, and their expression is controlledby CIA5, a transcription factor that controls many CCM genes.YFP fusions demonstrate that all three proteins are located onthe thylakoid membrane, and interactome studies indicate thatthey might associate with chloroplast CCM components. A singlemutant defective in ...
Fusion of green fluorescent protein (GFP) to proteins is a powerful method to investigate dynamic processes in vivo. The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP-tagging of proteins was employed in order to analyse proteins of the flagellar basal apparatus. Striated fiber assembling (SFA), centrin and deflagellation induced protein of 13 kDa (DIP13) were tagged with GFP at the C-terminal domain. In addition SFA was tagged at the N-terminal domain. The chimeric genes were stably transformed in C. reinhardtii. SFA is the mayor component of the striated microtubule associated fibers (SMAFS). GFP tagged SFA was incorporated into this fibers. N-terminal tagged SFA had similar properties like the wild-type protein. The length of the fibers increased with the strength of expression. The head domain of SFA is essential for fiber formation and photobleaching experiments did not show a pronounced dynamic of the fibers. The ...
View Stock Photo of The Green Algae Chlamydomonas With Its Two Flagella Sem X6000. Find premium, high-resolution photos at Getty Images.
The maintenance of flagellar length is thought to require both anterograde and retrograde intraflagellar transport (IFT). particles. The IFT particles then associate into linear arrays known as IFT trains (Pigino et al., 2009), which move processively from the base of the flagellum out to the tip. This anterograde transport Mouse monoclonal to CD74(PE) is driven by kinesin-2, a heterotrimeric complex composed of the FLA10 and FLA8 motor subunits (Walther et al., 1994) and the buy BS-181 HCl kinesin-associated protein KAP (Cole et al., 1993; Mueller et al., 2005). After their anterograde motion to the flagellar tip, IFT particles rearrange into a new set of IFT trains that move back to the base of the flagellum. This retrograde transport is powered by cytoplasmic dynein 1b, a large complex composed of the heavy chain motor subunit DHC1b (Pazour et al., 1999a; Porter et al., 1999; Signor et al., 1999) and numerous smaller components including D1bLIC (Perrone et al., 2003; Schafer et al., 2003; Hou ...
Catalytic domain of the Protein Serine/Threonine Kinase, Chlamydomonas reinhardtii FA2 and similar domains. Serine/Threonine Kinases (STKs), Chlamydomonas reinhardtii FA2-like subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Chlamydomonas reinhardtii FA2-like subfamily belongs to the (NIMA)-related kinase (Nek) family. The Nek family includes seven different Chlamydomonas Neks (CNKs 1-6 and Fa2). This subfamily includes FA2 and CNK4. The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Chlamydomonas reinhardtii FA2 was discovered in a genetic screen for deflagellation-defective mutants. It is essential for basal-body/centriole-associated microtubule severing, and plays a role in cell cycle progression. No cellular ...
Our initial mapping data placed Mcd4 into two possible locations. In both cases, it was desirable to generate bacterial artificial chromosome (BAC) contigs for eventual complementation, as well as additional markers. As a case study, we describe how BAC contigs can be extended using Chlamydomonas resources and our experience in generating site-specific markers.. Several BAC libraries have been constructed for Chlamydomonas, two of which are available through the Clemson Genomics Institute (http://www.genome.clemson.edu/groups/bac/). In addition, BAC contigs (http://www.biology.duke.edu/chlamy_genome/BAC/index.html) have been assembled for most of the STS and RFLP markers. In the case of mcd4, Gsp1 resides on scaffold 2 and CNA45 on scaffold 66. A 41-BAC contig exists for scaffold 2 covering approximately 1,000 kb (R. Nguyen, personal communication), and a smaller contig is linked to scaffold 66 and CNA45 (Fig. 6). An unknown amount of DNA separates scaffolds 2 and 66; such discontinuities in the ...
TY - JOUR. T1 - Chlamydomonas shortens its flagella by activating axonemal disassembly, stimulating IFT particle trafficking, and blocking anterograde cargo loading. AU - Pan, Junmin. AU - Snell, William J.. N1 - Funding Information: We thank Drs. Fred Grinnell, Joachim Seeman, Masahide Kikkawa, and Olivier Belzile for critical reading of the manuscript. We are especially grateful to Joel Rosenbaum, Dennis Diener, Lotte Pedersen, and George Witman for their generosity in providing us with antibodies, which were invaluable for these studies. We are greatly indebted to Dr. Joachim Seeman for his assistance with the live cell imaging and to Dr. Paul Nakonezny for statistical advice. This work was supported by grant GM25661 from the National Institutes of Health. PY - 2005/9. Y1 - 2005/9. N2 - Almost all eukaryotic cells form cilia/flagella, maintain them at their genetically specified lengths, and shorten them. Here, we define the cellular mechanisms that bring about shortening of flagella prior to ...
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Molecular motors in living cells are involved in whole-cell locomotion, contractility, developmental shape changes, and organelle movement and positioning. Whether motors of different directionality are functionally coordinated in cells or operate in a semirandom tug of war is unclear. We show here that anterograde and retrograde microtubule-based motors in the flagella of Chlamydomonas are regulated such that only motors of a common directionality are engaged at any single time. A laser trap was used to position microspheres on the plasma membrane of immobilized paralyzed Chlamydomonas flagella. The anterograde and retrograde movements of the microsphere were measured with nanometer resolution as microtubule-based motors engaged the transmembrane protein FMG-1. An average of 10 motors acted to move the microsphere in either direction. Reversal of direction during a transport event was uncommon, and quiescent periods separated every transport event, suggesting the coordinated and exclusive ...
Chlamydomonas PsbK protein: required for the stability of the photosystem II complex and essential for photoautotrophic growth; stability requirements of the photosynthetic complexes differ between cyanobacteria and C. reinhardtii; MW 4 kDa; has been sequenced
Single-cell alga Chlamydomonas, are able to generate cellular energy from the breakdown of sugars without taking up oxygen. They do this using a variety...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Probable component of the axonemal radial spoke head (By similarity). Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk, which is attached to a subfiber of the outer doublet microtubule, and a bulbous head, which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
This model does not account for two published observations: An mt+ strain carrying the MID gene transposed to an autosome differentiates as minus, as do mt+ cells transformed with the MID gene, even though neither possesses a copy of the MTD1 gene (Ferris and Goodenough 1997). To reconcile these observations with the results reported here, we are led to propose that plus gametes express a system, the MTD1-equivalent system, that is functionally equivalent to the MTD1 system but achieves this outcome without requiring the Mtd1 protein itself. When MID is introduced into a plus background, the MTD1-equivalent system enables sufficiently high MID expression to allow transformants to undergo minus differentiation, albeit success is usually incomplete (see results and Ferris and Goodenough 1997), meaning that the MTD1-equivalent system is not repressible by Mid. Importantly, at least one essential gene in the posited plus MTD-equivalent system must be resident in the MT+ locus. If the system were ...
https://version-10-5.string-db.org/cgi/network.pl?all_channels_on=1&block_structure_pics_in_bubbles=0&direct_neighbor=1&hide_disconnected_nodes=0&hide_node_labels=0&network_display_mode=svg&network_flavor=evidence&targetmode=proteins&identifier=3055. ...
https://version-10-5.string-db.org/cgi/network.pl?all_channels_on=1&block_structure_pics_in_bubbles=0&direct_neighbor=1&hide_disconnected_nodes=0&hide_node_labels=0&network_display_mode=svg&network_flavor=evidence&targetmode=proteins&identifier=3055. ...
For example, the E. coli moves by rotating its helical flagellum, Chlamydomonas flagella have a breaststroke kind of motion. ... Mitchell, David R. (2001). "Chlamydomonas flagella". Journal of Phycology. 36 (2): 261-273. doi:10.1046/j.1529-8817.2000.99218. ...
Snow algae species such as Chloromonas sp., Chlamydomonas sp., and Chlorella sp. are found in polar environments. Some ...
Gutman, Benjamin L.; Niyogi, Krishna K. (2004-06-01). "Chlamydomonas and Arabidopsis. A Dynamic Duo". Plant Physiology. 135 (2 ... model alga Chlamydomonas reinhardtii, and soybean as its "flagship" species for plant genomics geared towards bioenergy ... thaliana along with the alga Chlamydomonas reinhardtii.) Prior to this, a handful of A. thaliana geneticists had become HHMI ...
The green algae Chlamydomonas snowiae is named after her, as is the bacterial Genus Snowella. Her older brother, Dr. Benjamin ... Guiry, M. D. (April 11, 2002). "Chlamydomonas snowiae Printz". AlgaeBase. National University of Ireland, Galway. Retrieved ...
Euglena and Chlamydomonas). This flagella-associated structure observed in the large selenomonad can perhaps best be described ...
Examples: Chlamydomonas ("cloak unit"); Pseudomonas ("false unit"); Metamonad ("encompassing unit") -morph: Pronunciation: / ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-7. doi:10.1104/pp.010807. PMC 1540183. PMID 11743094. ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
"Chlamydomonas Ehrenberg, 1833: 288". algaeBASE. Retrieved 19 May 2013. "Spirogyra Link, 1820: 5". algaeBASE. Retrieved 19 May ... Rochaix JD (1998). The molecular biology of chloroplasts and mitochondria in Chlamydomonas. Dordrecht [u.a.]: Kluwer Acad. Publ ... Chlamydomonas), a ribbon-like spiral around the edges of the cell (e.g., Spirogyra), or slightly twisted bands at the cell ... "Ultrastructure of DNA-containing areas in the chloroplast of Chlamydomonas". J. Cell Biol. 13 (3): 383-91. doi:10.1083/jcb.13.3 ...
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
Chlamydomonas, Eudorina or Oogamous e.g. Chlamydomonas, Volvox.[clarification needed] Chlamydomonas has all three types of ... The chloroplast may be discoid, cup-shaped (e.g. Chlamydomonas), spiral or ribbon shaped[example needed] Most chlorophytes have ...
Crutchfield A, Diller K, Brand J (1999-02-01). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal ...
Chlamydomonas reinhardtii and Volvox carteri). The latter implies that UVR8 potentially appeared before the evolutionary split ...
This location is one of the most southerly locations where red snow algae (Chlamydomonas sp., Chloromonas sp., and ... Chlamydomonas nivalis) are found. The location is favourable for vegetation growth because of warm summer temperatures. Its ...
Chlamydomonas reinhardtii) and archaea (e.g., Methanococcus jannaschii). The proteins are of about 450 amino acyl residues in ...
Harz H, Hegemann P (1991-06-06). "Rhodopsin-regulated calcium currents in Chlamydomonas". Nature. 351 (6326): 489-491. Bibcode: ... they could demonstrate that a single gene from the alga Chlamydomonas produced large photocurrents when expressed in the oocyte ...
Johnson KA, Rosenbaum JL (December 1992). "Polarity of flagellar assembly in Chlamydomonas". The Journal of Cell Biology. 119 ( ... Drosophila melanogaster and Chlamydomonas reinhardtii. In mammals, disruption of the transition zone reduces the ciliary ...
Photocurrents of the Chlorophyceae Heamatococcus pluvialis and Chlamydomonas reinhardtii were studied over many years in the ... Harz H, Hegemann P (June 1991). "Rhodopsin-regulated calcium currents in Chlamydomonas". Nature. 351 (6326): 489-491. Bibcode: ... Kateriya, S. Fuhrmann, M. Hegemann, P.: Direct Submission: Chlamydomonas reinhardtii retinal binding protein (cop4) gene; ... February 2003). "Archaeal-type rhodopsins in Chlamydomonas: model structure and intracellular localization". Biochemical and ...
"Radial spoke proteins of Chlamydomonas flagella". Journal of Cell Science. 119 (Pt 6): 1165-74. doi:10.1242/jcs.02811. PMC ...
based on the Chlamydomonas reinhardtii genome. It has 866 unique ORFs, 1862 metabolites, 2499 gene-enzyme-reaction-association ... a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome". BMC Genomics. 12 Suppl 4: S5. ...
Ensuing studies 20 years after the identification of the same mutant strain of Chlamydomonas reinhardtii found that the ... When a photorespiratory mutant of the eukaryotic green alga Chlamydomonas reinhardtii was studied, the mutant strain was ... Suzuki K, Marek LF, Spalding MH (May 1990). "A photorespiratory mutant of Chlamydomonas reinhardtii". Plant Physiology. 93 (1 ... "Transcriptional Analysis of the Three Phosphoglycolate Phosphatase Genes in Wild Type and the pgp1 Mutant of Chlamydomonas ...
As of 1956, she reported on the photosynthetic products of Chlamydomonas. In January 1957 she was listed by the Phycological ... Allen, Mary Belle (1 June 1956). "Excretion of organic compounds by Chlamydomonas". Archiv für Mikrobiologie. 24 (2): 163-168. ...
Staub M, Denes G (1966). "Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. I Purification and properties of ...
"Entrez Gene: intraflagellar transport 74 homolog (Chlamydomonas)". Bell SE, Mavila A, Salazar R, Bayless KJ, Kanagala S, ...
Finst, Rip J.; Kim, Peter J.; Quarmby, Lynne M. (1998). "Genetics of the deflagellation pathway in Chlamydomonas". Genetics. ... Through her research using the single-celled ciliated green alga Chlamydomonas reinhardtii as a model organism, Quarmby ... "The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during ...
The unicellular green alga, Chlamydomonas reinhardtii grows as vegetative cells in nutrient rich growth medium, but depletion ... Sager R, Granick S (July 1954). "Nutritional control of sexuality in Chlamydomonas reinhardi". The Journal of General ...
Chlamydomonas reinhardtii, a unicellular relative of V. carteri, is known to possess genes related to regA. This suggests that ... V. carteri forms small spherical colonies, or coenobia, of 2000-6000 Chlamydomonas-type somatic cells and 12-16 large, ... Miller, Stephen M. (2010). "Volvox, Chlamydomonas, and the Evolution of Multicellularity". Nature Education. 3 (9): 65. ...
Schimmer, O; Kühne, I (1991). "Furoquinoline alkaloids as photosensitizers in Chlamydomonas reinhardtii". Mutation Research. ...
Chlamydomonas acidophila Chlamydomonas caudata Wille Chlamydomonas ehrenbergii Gorozhankin Chlamydomonas elegans G.S.West 1915 ... Chlamydomonas moewusii Chlamydomonas nivalis Chlamydomonas ovoidae Chlamydomonas reinhardtii Chlamydomonas is widely ... Chlamydomonas Center Chlamydomonas reinhardtii Transcription Factor Database Archived 2009-04-25 at the Wayback Machine 3D ... ISBN 978-1-904890-31-7 Harris, Elizabeth H. ( 2009) "The Genus Chlamydomonas" In The Chlamydomonas Sourcebook (Second Edition ...
Chlamydomonas eugametos w as i s o l a t e d i n 1929 b y MOEWUS (1931). A l t h o u g h t h e r e is s o m e q u e s t i o n a ... Comparative analysis of the mitochondrial genomes of Chlamydomonas eugametos and Chlamydomonas moewusii ... SummaryWe report the cloning and physical mapping of the mitochondrial genome of Chlamydomonas eugametos together with a… ... Influence of sulphate on the reduction of cadmium toxicity in the microalga Chlamydomonas moewusii. ...
During Zn limitation, Chlamydomonas reinhardtii hyperaccumulates Cu, a process dependent on the nutritional Cu sensor CRR1, but ... techniques demonstrate that Zn availability influences Cu+ storage and localization in the green alga Chlamydomonas, with Zn ... Harris, E.H., Stern, D.B. & Witman, G.B. The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and its Laboratory Use ( ... Knock-down of the COX3 and COX17 gene expression of cytochrome c oxidase in the unicellular green alga Chlamydomonas ...
We show here that the unicellular alga Chlamydomonas does indeed use a rhodopsin photoreceptor … ... A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas Nature. 1984 Oct;311(5988 ... We show here that the unicellular alga Chlamydomonas does indeed use a rhodopsin photoreceptor. We incorporated analogues of ...
... in the nucleus is an attractive alternative for the expression of recombinant proteins in the green microalga Chlamydomonas re ... Chlamydomonas annotation team, JGI annotation team, Grigoriev IV, Rokhsar DS, Grossman AR (2007) The Chlamydomonas genome ... Park S, Lee Y, Lee JH, Jin E (2013) Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii ... A novel salt-inducible CrGPDH3 promoter of the microalga Chlamydomonas reinhardtii for transgene overexpression. *Anayeli ...
Chlamydomonas spp.. Reference. Knut Yngve Borsheim & Gunnar Bratbak, Cell volume to cell carbon conversion factors for a ...
Chlamydomonas moewusii , Media: Soil Extract Medium , Origin: , Type Culture: No , Isolation: R.A. Lewin , Isolator Number: ... Deadline to place an order for UTEX 695 Chlamydomonas moewusii with a 2022 shipment date has passed. New orders will not ship ...
Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Expression Regulated by Carbon Dioxide and the CO2-Concentrating ...
Green algae Chlamydomonas reinhardtii. Reference. Boyle NR, Morgan JA. Flux balance analysis of primary metabolism in ... Chlamydomonas reinhardtii. BMC Syst Biol. 2009 Jan 7 3: 4. p.6 of free online article - table 2PubMed ID19128495 ...
Structure of a vegetative Chlamydomonas reinhardtii cell.. This cell has a 5-10 µm diameter (Gallaher et al., 2015). The two ... The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in ... The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in ... The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in ...
Dive into the research topics of Chlamydomonas genetics, a tool for the study of bioenergetic pathways. Together they form a ...
A. Tadiello_Chlamydomonas Nivalis_exhibition view_ph Danilo Donzelli. Published ottobre 11, 2021 at 1200 × 800 in Alberto ...
List of publications , Photosynthetic hydrogen production as acclimation mechanism in nutrient-deprived Chlamydomonas *Home ... In the current study, the role of hydrogen photoproduction in the acclimation of Chlamydomonas reinhardtii cells to nutrient ...
Uniacke J, Zerges W(2008) Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii. J Cell ... Khrebtukova I, Spreitzer RJ(1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5- ... Margulies MM, Michaels A(1974) Ribosomes bound to chloroplast membranes in Chlamydomonas reinhardtii. J Cell Biol 60:65-77. 44 ... Uniacke, James and Zerges, William (2009) Chloroplast protein targeting involves localized translation in Chlamydomonas. ...
By optimizing nitrate supply and cell density, a one-step cultivation system for Chlamydomonas sp. KAC1801 under nitrate- ... We previously developed a mutant Chlamydomonas sp. KAC1801, which can accumulate lipids irrespective of the presence or absence ... Protein ID (Chlamydomonas reinhardtii) Gene IDs assigned by AUGUSTUS Product Gene name logFC p-Value FDR ... From: Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi- ...
The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of its photosynthetic ability, cilia- ... d) Chlamydomonas cells grown in TAP with 0.5% DMSO. (e) Cells grown in TAP with 25 µM fluperlapine. (f) Fluperlapine-induced ... Effects of fluperlapine on Chlamydomonas. (a-c) Dibenzazapine analogs: (a) fluperlapine; (b) clozapine; (c) clothiapine. Common ... g) Flagellar regeneration of Chlamydomonas in solvent (closed squares) or 100 µM fluperlapine (open squares). Length of at ...
A new mutant strain of Chlamydomonas, ptx1, has been identified which is defective in phototaxis. This strain swims with a rate ... ptx1, a nonphototactic mutant of Chlamydomonas, lacks control of flagellar dominance. C J Horst, C J Horst ... C J Horst, G B Witman; ptx1, a nonphototactic mutant of Chlamydomonas, lacks control of flagellar dominance.. J Cell Biol 1 ... The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella ...
Chlamydomonas reinhardi. 68 µmol/L. CUGED5 10,393,1986. gene conversion and mitotic recombination. oral/Drosophila melanogaster ...
Chlamydomonas Ortholog Ciliary Ultrastructure in Subjects with Biallelic Mutations Presence of Laterality Defects ...
Chlamydomonas reinhardtii - RPL38. Species. Chlamydomonas reinhardtii. Gene Name. RPL38. 5 Upstream. ...
Thus, a flow cytometry-based strategy was used to study the adaptive evolution in Chlamydomonas reinhardtii wild-type strain ... programmed adaptive evolution reveals potential role of carbon and nitrogen pathways during lipid accumulation in Chlamydomonas ...
The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas ... The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas ... The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas ... The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas ...
AGO1-PAZ , Argonaute 1 PAZ domain (Chlamydomonas) AS14 2797 , clonality: polyclonal , host: rabbit , reactivity: Chlamydomonas ... AGO1 , Argonaute 1 (Chlamydomonas) AS14 2776 , Clonality: Polyclonal , Host: Rabbit , Reactivity: Chlamydomonas reinhardii. 15 ... AGO3-PAZ , Argonaute 3 PAZ domain (Chlamydomonas) AS14 2799 , clonality: polyclonal , host: rabbit , reactivity: Chlamydomonas ... Home / Antibodies Plant/Algal / DNA/RNA/Cell Cycle / plant RNA / Anti-AGO1 , argonaute 1 (Chlamydomonas) antibodies ...
intraflagellar transport 80 homolog (Chlamydomonas). *KIAA1374. *MGC126543. *WD repeat domain 56. *WD repeat-containing protein ...
... sexual reproduction of Chlamydomonas is discussed here . Chlamydomonas structure has 150 species. It is the commonest ... Origin of differentiation of sex in Chlamydomonas. Chlamydomonas shows origin and differentiation of sex. The zoospores and ... Structure of Chlamydomonas. Its vegetative stage has very simple structure. Its size is about 0.02 mm. Each cell is spherical, ... Chlamydomonas has 150 species. It is the commonest unicellular Volvocales. It is one of the simplest unicellular widely ...
Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022. *Chlamydomonas Spatial Interactome (CSI) Collection, ... We will only send out a few select emails a year that are considered important for the Chlamydomonas community. ... We will only send out a few select emails a year that are considered important for the Chlamydomonas community. ... Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green ...
Generation of Expressed Sequence Tags from low-CO2 and high-CO 2 adapted cells of Chlamydomonas reinhardtii DNA Research. , 7, ... A Large Scale Structural Analysis of cDNAs in a Unicellular Green Alga, Chlamydomonas reinhardtii. I. Generation of 3433 Non- ... Establishment of Publicly Available cDNA Material and Information Resource of Chlamydomonas reinhardtii (Chlorophyta), to ...
  • EIT-Food and Algae-UK are pleased to provide funding for the upcoming workshop "Expressing transgenes in Chlamydomonas and Phaeodactylum - a guide to commonly used methods for transformation of their nuclear genomes", to be held at the Department of Plant Sciences of the University of Cambridge. (cam.ac.uk)
  • Previous work has raised the possibility that these algae might use a novel pathway because the green alga Chlamydomonas reinhardtii was shown to possess a mevalonate-independent methylerythritol 4-phosphate not present in fungi. (elsevier.com)
  • The cilia on the algae Chlamydomonas reinhardtii look like two antennae on an ant's head. (kumc.edu)
  • The effects data-set from Pseudokirchernella subcapitata was used for the development of an algae BLM (De Schamphelaere et al, 2003) The capacity of the BLM for predicting copper toxicity to other algae species was demonstrated from copper toxicity studies with Chlamydomonas reinhardti and Chlorella vulgaris (De schamplelaere and Janssen, 2006). (europa.eu)
  • Using energy from sunlight and electrons from water, both the green algae Chlamydomonas reinhardtii and the cyanobacterium Synechocystis sp. (nrel.gov)
  • Maintain Tardigrades by feeding them Chlamydomonas or other single-celled green algae. (flinnsci.com)
  • Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. (novusbio.com)
  • We actually grow our little green algae called Chlamydomonas Reinhardtii and the way that we grow this algae is to grow it in stainless steel fermenters. (coursera.org)
  • led the group "Dynamics of Fluid and Biological Interfaces" from 2013 to 2021, with which he achieved ground-breaking research results on the behavior of algae ( Chlamydomonas Reinhardtii ) under variable light conditions. (mpg.de)
  • In plants and algae, the serine/threonine kinase STN7/STT7, orthologous protein kinases in Chlamydomonas reinhardtii and Arabidopsis (Arabidopsis thaliana), respectively, is an important regulator in acclimation to changing light environments. (unige.ch)
  • Chlamydomonas acidophila Chlamydomonas caudata Wille Chlamydomonas ehrenbergii Gorozhankin Chlamydomonas elegans G.S.West 1915 Chlamydomonas moewusii Chlamydomonas nivalis Chlamydomonas ovoidae Chlamydomonas reinhardtii Chlamydomonas is widely distributed in freshwater or damp soil. (wikipedia.org)
  • Franz Josef Land red snow metagenomes confirmed that the communities are composed of the autotroph Chlamydomonas nivalis that is supporting a complex viral and heterotrophic bacterial community. (peerj.com)
  • Here, we report the structural identification of a photosystem I (PSI)-light harvesting complex I (LHCI)-cytochrome (cyt) b 6 f supercomplex isolated from the unicellular alga Chlamydomonas reinhardtii under anaerobic conditions, which induces CEF. (elsevier.com)
  • This gene encodes a protein that appears to be a component the radial spoke head, as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. (nih.gov)
  • Subsequently, we apply our method to the recently sequenced green alga Chlamydomonas reinhardtii . (rsc.org)
  • The nuclear proteome of the green alga Chlamydomonas reinhardtii. (mpg.de)
  • Both 5mC and 6mA DNA modifications were identified in the unicellular green alga Chlamydomonas reinhardtii in 1978, but their functions were unknown. (the-scientist.com)
  • In their new study, the researchers employed a light-sensitive protein from the green alga Chlamydomonas reinhardtii. (phys.org)
  • Subsequently, we will study the biotransformation and toxicokinetics of a set of compounds in selected algal strains (standard strains of the green alga Chlamydomonas reinhardtii and the cyanobacterium Microcystis aeruginosa, and a triclosan-resistant strain of Microcystis aeruginosa) in laboratory experiments. (eawag.ch)
  • A combined 18S rDNA and rbcL phylogenetic analysis of Chloromonas and Chlamydomonas (Chlorophyceae, Volvocales ) emphasizing snow and other cold-temperature habitats. (wikipedia.org)
  • Molecular phylogeny and taxonomic revision of Chlamydomonas (Chlorophyta). (wikipedia.org)
  • Chlamydomonas reinhardti, Chlorella vulgaris and Pseudokirchernella subcapitata. (europa.eu)
  • Chlamydomonas is used as a model organism for molecular biology, especially studies of flagellar motility and chloroplast dynamics, biogenesis, and genetics. (wikipedia.org)
  • We used fluorescence confocal microscopy to explore the targeting mechanisms used by several chloroplast proteins in the green alga Chlamydomonas. (concordia.ca)
  • 2005) Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast. (concordia.ca)
  • 12.Chua NH, Blobel G, Siekevitz P, Palade GE(1976) Periodic variations in the ratio of free to thylakoid-bound chloroplast ribosomes during the cell cycle of Chlamydomonas reinhardtii. (concordia.ca)
  • Light-inducible gene HSP70B encodes a chloroplast-localized heat shock protein in Chlamydomonas reinhardtii. (mpg.de)
  • Some regulatory systems of Chlamydomonas are more complex than their homologs in Gymnosperms, with evolutionarily related regulatory proteins being larger and containing additional domains. (wikipedia.org)
  • The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas. (wikipedia.org)
  • The expression of transgenes in the nucleus is an attractive alternative for the expression of recombinant proteins in the green microalga Chlamydomonas reinhardtii . (springer.com)
  • 13.Margulies MM(1983) Synthesis of photosynthetic membrane proteins directed by RNA from rough thylakoids of Chlamydomonas reinhardtii. (concordia.ca)
  • Total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. (agrisera.com)
  • We sequenced the similar to 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. (pugetsound.edu)
  • Finally, ubiquitinated proteins accumulate in cilia of mammalian photoreceptors and Chlamydomonas cells when BBSome function is compromised. (biorxiv.org)
  • Molecular phylogeny studies indicated that the traditional genus Chlamydomonas as defined using morphological data was polyphyletic within Volvocales. (wikipedia.org)
  • The study of Brack and Rottler (1994) was conducted on freshwater living species Chlamydomonas reinhardtii , with an exposure duration of 72 h in a static test system. (europa.eu)
  • The doctoral candidate from India, who came to Jena to do his Master's degree in Molecular Life Sciences, examines the species Chlamydomonas reinhardtii on a microscope slide. (news-medical.net)
  • So although we work with a specific species Chlamydomonas Reinhardtii there's different versions of that species and you really have to identify which of these species can grow under heterotrophic conditions in a fermenter. (coursera.org)
  • By combining experiments on three different species-Chlamydomonas reinhardtii, Tetraselmis subcordiforms, and Oxyrrhis marina-with simulations and analytical modeling, we show that the fundamental physical process regulating proximity to a swimming microorganism is hydrodynamic particle entrainment. (warwick.ac.uk)
  • In Chlamydomonas, three different protocols can be used to obtain isolated basal body complexes. (wustl.edu)
  • Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella. (pugetsound.edu)
  • In this study, we find that proper flagellar length control in Chlamydomonas reinhardtii requires the activity of a new member of the cyclin-dependent kinase (CDK) family, which is encoded by the LF2 (long flagella 2) gene. (okstate.edu)
  • Tam, LW , Wilson, NF & Lefebvre, PA 2007, ' A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas ', Journal of Cell Biology , vol. 176, no. 6, pp. 819-829. (okstate.edu)
  • Cells can glide along surfaces using any type of mechanism including the flagellated Chlamydomonas , which propels themselves along a surface with its flagella. (hypertextbook.com)
  • Influence of sulphate on the reduction of cadmium toxicity in the microalga Chlamydomonas moewusii. (semanticscholar.org)
  • Sulphate, more than a nutrient, protects the microalga Chlamydomonas moewusii from cadmium toxicity. (semanticscholar.org)
  • Deadline to place an order for UTEX 695 Chlamydomonas moewusii with a 2022 shipment date has passed. (utex.org)
  • Cobamide remodeling in the freshwater microalga Chlamydomonas reinhardtii. (mpg.de)
  • ISBN 978-1-904890-31-7 Harris, Elizabeth H. ( 2009) "The Genus Chlamydomonas" In The Chlamydomonas Sourcebook (Second Edition), chapter 1, volume 1, pages 1-24. (wikipedia.org)
  • Harris, Elizabeth H. The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use . (hypertextbook.com)
  • In: The Chlamydomonas Sourcebook, Second Edition, Bd. (mpg.de)
  • Virtanen O, Khorobrykh S, Tyystjärvi E (2021) Acclimation of Chlamydomonas reinhardtii to extremely strong light. (copasi.org)
  • We show here that the unicellular alga Chlamydomonas does indeed use a rhodopsin photoreceptor. (nih.gov)
  • The chloroplastic GrpE homolog of Chlamydomonas: Two isoforms generated by differential splicing. (mpg.de)
  • This gene is the human homolog of the Chlamydomonas inner dynein arm gene, p28. (novusbio.com)
  • The distinctions with similar and/or closely related species, such as Chlamydomonas globosa J. Snow and Chlamydomonas orbicularis E. G. Pringsh. (elsevier.com)
  • Pioneering studies carried out in the green alga Chlamydomonas established the link between cilia and several genetic diseases. (pasteur.fr)
  • explore this question using the single-celled alga Chlamydomonas. (plantae.org)
  • It was Chlamydomonas reinhardtii , a one-celled alga often used in research to study many cell behaviors such as photosynthesis, biofuel production and the evolution of single-celled organisms into multicellular ones. (kumc.edu)
  • The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. (mpg.de)
  • Transcriptome-Wide Changes in Chlamydomonas reinhardtii Gene Ex" by Wei Fang, Yaqing Si et al. (unl.edu)
  • VChR2 is similar to the ChR2 counterpart from Chlamydomonas reinhardtii with respect to its absorption maximum ( 460 nm) and photocycle dynamics. (uni-bielefeld.de)
  • Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School). (chlamycollection.org)
  • Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. (pugetsound.edu)
  • Green transcription factors: A Chlamydomonas overview. (mpg.de)
  • Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas reinhardtii. (umassmed.edu)
  • Dissecting the sequential assembly and localization of intraflagellar transport particle complex B in Chlamydomonas. (tamu.edu)
  • abstract = "Chlamydomonas reinhardtii P. A. Dang. (elsevier.com)
  • I. Emendation of Chlamydomonas Ehrenberg and Chloromonas Gobi, and descripription of Oogamochlamys gen. nov. and Lobochlamys gen. nov. (wikipedia.org)
  • Die Gattung Chlamydomonas Ehrenberg (Chlamydomonas und die nächstverwandten Gattungen III). (antiqbook.com)
  • The microtubule organizing center (MTOC) in Chlamydomonas is defined as the pair of basal bodies, the rootlet microtubules, the distal and proximal striated fibers, and the nucleobasal body connectors (NBBCs). (wustl.edu)
  • Chlamydomonas reinhardtii have elucidated some novel metabolically important genes and this enhances the lipid production in this microalgae. (frontiersin.org)
  • We suggest specific genes in the genome of Chlamydomonas which are the strongest candidates for coding the responsible enzymes. (rsc.org)
  • A suspension of gyrotactic microalgae Chlamydomonas augustae swimming in a cylindrical water vessel in solid-body rotation is studied. (arxiv.org)
  • Modeling of Chlamydomonas reinhardtii motility in confinement. (mpg.de)
  • In the current study, the role of hydrogen photoproduction in the acclimation of Chlamydomonas reinhardtii cells to nutrient deficiency was investigated. (utu.fi)
  • The Chlamydomonas Genome Reveals The Evolution Of Key Animal And Plant" by Sabeeha S. Merchant, Simon E. Prochnik et al. (pugetsound.edu)
  • Jonikas and his team discovered that in their model alga Chlamydomonas, this clustering of Rubisco is mediated by a protein they called EPYC1 for Essential Pyrenoid Component 1. (carnegiescience.edu)
  • Baek K, Lee Y, Nam O, Park S, Sim SJ, Jin E (2016) Introducing Dunaliella LIP promoter containing light-inducible motifs improves transgenic expression in Chlamydomonas reinhardtii . (springer.com)
  • A new mutant strain of Chlamydomonas, ptx1, has been identified which is defective in phototaxis. (rupress.org)
  • Casais-Molina ML, Peraza-Echeverria S, Echevarría-Machado I, Herrrera-Valencia VA (2016) Expression of Chlamydomonas reinhardtii CrGPDH2 and CrGPDH3 cDNAs in yeast reveals that they encode functional glycerol-3-phosphate dehydrogenases involved in glycerol production and osmotic stress tolerance. (springer.com)
  • 185. Brzezowski P, Morhard MK, Dent RM, Niyogi KK, Grimm B. (2016) CHLI2 cannot substitute for CHLI1, which is indispensable for the activity of Mg chelatase in Chlamydomonas reinhardtii J Exp Bot. (hu-berlin.de)