A genus GREEN ALGAE in the order VOLVOCIDA. It consists of solitary biflagellated organisms common in fresh water and damp soil.
A species of GREEN ALGAE. Delicate, hairlike appendages arise from the flagellar surface in these organisms.
Proteins found in any species of algae.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
Ribonucleic acid in algae having regulatory and catalytic roles as well as involvement in protein synthesis.
A phylum of photosynthetic EUKARYOTA bearing double membrane-bound plastids containing chlorophyll a and b. They comprise the classical green algae, and represent over 7000 species that live in a variety of primarily aquatic habitats. Only about ten percent are marine species, most live in freshwater.
A family of multisubunit cytoskeletal motor proteins that use the energy of ATP hydrolysis to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria.
A bundle of MICROTUBULES and MICROTUBULE-ASSOCIATED PROTEINS forming the core of each CILIUM or FLAGELLUM. In most eukaryotic cilia or flagella, an axoneme shaft has 20 microtubules arranged in nine doublets and two singlets.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Cytochromes f are found as components of the CYTOCHROME B6F COMPLEX. They play important role in the transfer of electrons from PHOTOSYSTEM I to PHOTOSYSTEM II.
Deoxyribonucleic acid that makes up the genetic material of algae.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
A protein complex that includes CYTOCHROME B6 and CYTOCHROME F. It is found in the THYLAKOID MEMBRANE and plays an important role in process of PHOTOSYNTHESIS by transferring electrons from PLASTOQUINONE to PLASTOCYANIN or CYTOCHROME C6. The transfer of electrons is coupled to the transport of PROTONS across the membrane.
A large multisubunit protein complex found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to catalyze the splitting of WATER into DIOXYGEN and of reducing equivalents of HYDROGEN.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Proteins found in any species of protozoan.
The functional hereditary units of protozoa.
A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Protein complexes that take part in the process of PHOTOSYNTHESIS. They are located within the THYLAKOID MEMBRANES of plant CHLOROPLASTS and a variety of structures in more primitive organisms. There are two major complexes involved in the photosynthetic process called PHOTOSYSTEM I and PHOTOSYSTEM II.
A copper-containing plant protein that is a fundamental link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
Membranous cisternae of the CHLOROPLAST containing photosynthetic pigments, reaction centers, and the electron-transport chain. Each thylakoid consists of a flattened sac of membrane enclosing a narrow intra-thylakoid space (Lackie and Dow, Dictionary of Cell Biology, 2nd ed). Individual thylakoids are interconnected and tend to stack to form aggregates called grana. They are found in cyanobacteria and all plants.
Complexes containing CHLOROPHYLL and other photosensitive molecules. They serve to capture energy in the form of PHOTONS and are generally found as components of the PHOTOSYSTEM I PROTEIN COMPLEX or the PHOTOSYSTEM II PROTEIN COMPLEX.
A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
A genus of GREEN ALGAE in the family Volvocaceae. They form spherical colonies of hundreds or thousands of bi-flagellated cells in a semi-transparent gelatinous ball.
Polyunsaturated side-chain quinone derivative which is an important link in the electron transport chain of green plants during the photosynthetic conversion of light energy by photophosphorylation into the potential energy of chemical bonds.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A pre-emergent herbicide.
The absence of light.
A highly branched glucan in starch.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Ribonucleic acid in chloroplasts having regulatory and catalytic roles as well as involvement in protein synthesis.
Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Cytochromes of the c type that are involved in the transfer of electrons from CYTOCHROME B6F COMPLEX and PHOTOSYSTEM I.
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins encoded by the CHLOROPLAST GENOME or proteins encoded by the nuclear genome that are imported to and resident in the CHOROPLASTS.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Cytochromes (electron-transporting proteins) with protoheme (HEME B) as the prosthetic group.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
Self-replicating, short, fibrous, rod-shaped organelles. Each centriole is a short cylinder containing nine pairs of peripheral microtubules, arranged so as to form the wall of the cylinder.
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
The relationships of groups of organisms as reflected by their genetic makeup.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The sum of the weight of all the atoms in a molecule.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
The functional hereditary units of PLANTS.
A family of zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide. They play an important role in the transport of CARBON DIOXIDE from the tissues to the LUNG. EC
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
An enzyme that catalyzes the transfer of glucose from ADPglucose to glucose-containing polysaccharides in 1,4-alpha-linkages. EC
The rate dynamics in chemical or physical systems.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Proteins found in the microtubules.
Nonmotile unicellular green algae potentially valuable as a source of high-grade protein and B-complex vitamins.
An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of gonorrhea.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A potassium salt used to replenish ELECTROLYTES, for restoration of WATER-ELECTROLYTE BALANCE, as well as a urinary and systemic alkalizer, which can be administered orally or by intravenous infusion. Formerly, it was used in DIURETICS and EXPECTORANTS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The branch of biology dealing with the effect of light on organisms.
Processes by which phototrophic organisms use sunlight as their primary energy source. Contrasts with chemotrophic processes which do not depend on light and function in deriving energy from exogenous chemical sources. Photoautotrophy (or photolithotrophy) is the ability to use sunlight as energy to fix inorganic nutrients to be used for other organic requirements. Photoautotrophs include all GREEN PLANTS; GREEN ALGAE; CYANOBACTERIA; and green and PURPLE SULFUR BACTERIA. Photoheterotrophs or photoorganotrophs require a supply of organic nutrients for their organic requirements but use sunlight as their primary energy source; examples include certain PURPLE NONSULFUR BACTERIA. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; HETEROTROPHY; chemotrophy; or phototrophy) to utilize different sources to meet their nutrients and energy requirements.
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.
Dyneins that are responsible for ciliary and flagellar beating.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An order of CHLOROPHYTA commonly found in freshwater habitats. Characteristics include the presence of a cellulose wall and two to four equal, smooth, apical flagella.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.
An autosomal recessive disorder characterized by a triad of DEXTROCARDIA; INFERTILITY; and SINUSITIS. The syndrome is caused by mutations of DYNEIN genes encoding motility proteins which are components of sperm tails, and CILIA in the respiratory and the reproductive tracts.
Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
An enzyme that catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX by the conversion of two propionate groups to two vinyl groups. It is the sixth enzyme in the 8-enzyme biosynthetic pathway of HEME, and is encoded by CPO gene. Mutations of CPO gene result in HEREDITARY COPROPORPHYRIA.
The quantity of volume or surface area of ORGANELLES.

The internal Cys-207 of sorghum leaf NADP-malate dehydrogenase can form mixed disulphides with thioredoxin. (1/808)

The role of the internal Cys-207 of sorghum NADP-malate dehydrogenase (NADP-MDH) in the activation of the enzyme has been investigated through the examination of the ability of this residue to form mixed disulphides with thioredoxin mutated at either of its two active-site cysteines. The h-type Chlamydomonas thioredoxin was used, because it has no additional cysteines in the primary sequence besides the active-site cysteines. Both thioredoxin mutants proved equally efficient in forming mixed disulphides with an NADP-MDH devoid of its N-terminal bridge either by truncation, or by mutation of its N-terminal cysteines. They were poorly efficient with the more compact WT oxidised NADP-MDH. Upon mutation of Cys-207, no mixed disulphide could be formed, showing that this cysteine is the only one, among the four internal cysteines, which can form mixed disulphides with thioredoxin. These experiments confirm that the opening of the N-terminal disulphide loosens the interaction between subunits, making Cys-207, located at the dimer contact area, more accessible.  (+info)

Chlamydomonas chloroplast ferrous hemoglobin. Heme pocket structure and reactions with ligands. (2/808)

We report the optical and resonance Raman spectral characterization of ferrous recombinant Chlamydomonas LI637 hemoglobin. We show that it is present in three pH-dependent equilibrium forms including a 4-coordinate species at acid pH, a 5-coordinate high spin species at neutral pH, and a 6-coordinate low spin species at alkaline pH. The proximal ligand to the heme is the imidazole group of a histidine. Kinetics of the reactions with ligands were determined by stopped-flow spectroscopy. At alkaline pH, combination with oxygen, nitric oxide, and carbon monoxide displays a kinetic behavior that is interpreted as being rate-limited by conversion of the 6-coordinate form to a reactive 5-coordinate form. At neutral pH, combination rates of the 5-coordinate form with oxygen and carbon monoxide were much faster (>10(7) microM-1 s-1). The dissociation rate constant measured for oxygen is among the slowest known, 0.014 s-1, and is independent of pH. Replacement of the tyrosine 63 (B10) by leucine or of the putative distal glutamine by glycine increases the dissociation rate constant 70- and 30-fold and increases the rate of autoxidation 20- and 90-fold, respectively. These results are consistent with at least two hydrogen bonds stabilizing the bound oxygen molecule, one from tyrosine B10 and the other from the distal glutamine. In addition, the high frequency (232 cm-1) of the iron-histidine bond suggests a structure that lacks any proximal strain thus contributing to high ligand affinity.  (+info)

Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas. (3/808)

A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR protocol, we recovered two cDhc sequences distinct from the axonemal Dhc sequences identified previously. cDhc1a is closely related to the major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor cDhc isoform identified in sea urchins, Caenorhabditis elegans, and Tetrahymena. The Chlamydomonas cDhc1b transcript is a low-abundance mRNA whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and identified two strains in which portions of the cDhc1b gene have been deleted. The two mutants assemble short flagellar stumps (<1-2 micrometer) filled with aberrant microtubules, raft-like particles, and other amorphous material. The results indicate that cDhc1b is involved in the transport of components required for flagellar assembly in Chlamydomonas.  (+info)

Cell division: The renaissance of the centriole. (4/808)

Centrioles are located at the center of the cytoskeleton and duplicate exactly once per cell cycle. Recent studies suggest that centrioles are required for the organization of a functional centrosome and that centriole assembly requires both gamma- and delta-tubulin.  (+info)

Intracellular motility: A special delivery service. (5/808)

Recent studies have identified a delivery service that operates in specialised cell appendages: two motor proteins and a novel protein organelle use axonemal microtubules as tracks to shuttle essential components to the tips of flagella and the dendrites of sensory neurons.  (+info)

Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. (6/808)

Eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. To identify new genes involved in the regulation of axonal transport in Drosophila melanogaster, we undertook a screen based upon the sluggish larval phenotype of known motor mutants. One of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis. These defects include intra-axonal accumulations of cargoes, severe axonal degeneration, and aberrant chromosome segregation. The gene identified by robl encodes a 97-amino acid polypeptide that is 57% identical (70% similar) to the 105-amino acid Chlamydomonas outer arm dynein-associated protein LC7, also reported here. Both robl and LC7 have homology to several other genes from fruit fly, nematode, and mammals, but not Saccharomyces cerevisiae. Furthermore, we demonstrate that members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. We propose that roadblock/LC7 family members may modulate specific dynein functions.  (+info)

Domains in the 1alpha dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas. (7/808)

Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1alpha Dhc of the I1 inner dynein arm. The 1alpha Dhc is similar to other axonemal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH(2)- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1alpha Dhcs were recovered. The truncated 1alpha Dhc fragments lacked the dynein motor domain, but still assembled with the 1beta Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1alpha motor domain in the I1 structure and further revealed the role of the 1alpha Dhc in flagellar motility and phototactic behavior.  (+info)

The complete mitochondrial DNA sequences of Nephroselmis olivacea and Pedinomonas minor. Two radically different evolutionary patterns within green algae. (8/808)

Green plants appear to comprise two sister lineages, Chlorophyta (classes Chlorophyceae, Ulvophyceae, Trebouxiophyceae, and Prasinophyceae) and Streptophyta (Charophyceae and Embryophyta, or land plants). To gain insight into the nature of the ancestral green plant mitochondrial genome, we have sequenced the mitochondrial DNAs (mtDNAs) of Nephroselmis olivacea and Pedinomonas minor. These two green algae are presumptive members of the Prasinophyceae. This class is thought to include descendants of the earliest diverging green algae. We find that Nephroselmis and Pedinomonas mtDNAs differ markedly in size, gene content, and gene organization. Of the green algal mtDNAs sequenced so far, that of Nephroselmis (45,223 bp) is the most ancestral (minimally diverged) and occupies the phylogenetically most basal position within the Chlorophyta. Its repertoire of 69 genes closely resembles that in the mtDNA of Prototheca wickerhamii, a later diverging trebouxiophycean green alga. Three of the Nephroselmis genes (nad10, rpl14, and rnpB) have not been identified in previously sequenced mtDNAs of green algae and land plants. In contrast, the 25,137-bp Pedinomonas mtDNA contains only 22 genes and retains few recognizably ancestral features. In several respects, including gene content and rate of sequence divergence, Pedinomonas mtDNA resembles the reduced mtDNAs of chlamydomonad algae, with which it is robustly affiliated in phylogenetic analyses. Our results confirm the existence of two radically different patterns of mitochondrial genome evolution within the green algae.  (+info)

Assembly of flagellar radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3 ...
Previous physiological and pharmacological experiments have demonstrated that the Chlamydomonas flagellar axoneme contains a cAMP-dependent protein kinase (PKA) that regulates axonemal motility and dynein activity. However, the mechanism for anchoring PKA in the axoneme is unknown. Here we test the hypothesis that the axoneme contains an A-kinase anchoring protein (AKAP). By performing RII blot overlays on motility mutants defective for specific axonemal structures, two axonemal AKAPs have been identified: a 240-kD AKAP associated with the central pair apparatus, and a 97-kD AKAP located in the radial spoke stalk. Based on a detailed analysis, we have shown that AKAP97 is radial spoke protein 3 (RSP3). By expressing truncated forms of RSP3, we have localized the RII-binding domain to a region between amino acids 144-180. Amino acids 161-180 are homologous with the RII-binding domains of other AKAPs and are predicted to form an amphipathic helix. Amino acid substitution of the central residues of ...
With 1 Figure in the Text. This work was conducted in the Department of Biological Sciences at Stanford University, and was supported in part by a Henry Newell Fellowship, and in part by a Research Fellowship (GF-6012) from the National Institutes of Health. ...
The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions - a spoke head, a bifurcated neck and a stalk. While the neck and the stalk are asymmetric, two-fold symmetry of the head has remained controversial.. In this work we used single particle cryo-EM analysis to generate 3D structure of the whole radial spoke at unprecedented resolution. We show the head region at 15Å resolution and confirm two-fold symmetry. Using distance constraints generated by crosslinking mass spectrometry we locate two components, RSP2 and 4 at the head/neck regions. Our biophysical analysis of isolated RSPs 4, 9, and 10 affirmed their oligomeric state. Our results enabled us to redefine the boundaries of the regions and propose a model of organization of the radial spoke component proteins. ...
We have shown that the Chlamydomonas mutants ida5 (Kato et al., 1993) and ida5-t, lacking four subspecies of inner-arm dynein, have mutations in an actin-encoding gene such that its gene product is totally lost. The axoneme of these mutants contain NAP instead of conventional actin, as evidenced by the two novel spots in two-dimensional electrophoresis patterns. These findings are unexpected since Chlamydomonas and a related species, Volvox, have been shown to have a single gene for actin, which is ∼90% homologous to rabbit skeletal muscle actin (Cresnar et al., 1990; Sugase et al., 1996). The fact that ida5 and ida5-t grow normally suggests either that NAP or other proteins may be able to substitute for conventional actin in important functions, or that actin is not essential for the growth of Chlamydomonas.. The molecular identity of NAP remains to be studied, but it appears to be somehow related to actin. This is because NAP and actin have similar molecular weights, can serve as the subunit ...
Amoroso, G., D. Sueltemeyer, C. Thyssen and H.P. Fock (1998). Uptake of HCO3- and CO2 in cells and chloroplasts from the microalgae Chlamydomonas reinhardtii and Dunaliella tertiolecta. Plant Physiol. 116, 193-201. Asleson, C.M. and P.A. Lefebvre (1998). Genetic analysis of flagellar length control in Chlamydomonas reinhardtii: A new long-flagella locus and extragenic suppressor mutations. Genetics 148, 693-702. Bhattacharya, D. and L. Medlin (1998). Algal phylogeny and the origin of land plants. Plant Physiol. 116, 9-15. Boschetti, A. and K. Schmid (1998). Energy supply for ATP-synthase deficient chloroplasts of Chlamydomonas reinhardii. Plant Cell Physiol. 39, 160-168. Brosch-Salomon, S., M. Hoeftberger, A. Holzinger and U. Luetz-Meindl (1998). Ultrastructural localization of polysaccharides and N-acetyl-D-galactosamine in the secretory pathway of green algae (Desmidiaceae). J. Exp. Bot. 49, 145-153. Calenberg, M., U. Brohsonn, M. Zedlacher and G. Kreimer (1998). Light- and Ca2+-modulated ...
Fingerprint Dive into the research topics of Role of timer and sizer in regulation of Chlamydomonas cell cycle. Together they form a unique fingerprint. ...
Molecular analysis of LC7 from the Chlamydomonas outer dynein arm. (A) Two tryptic peptides from outer arm dynein LC7 were completely sequenced, yielding a tota
Fertilization tubules in wild-type (A) and ida5 (B) mt+ gametes produced in response to a 1-h exposure to 10 mM dibutyryl-cAMP and 1 mM IBMX. Bar, 0.3 μm.
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Chlamydomonas monoica undergoes intraclonal mating-type differentiation (homothallism). Although the species differs in this regard from the more commonly studied heterothallic C. reinhardtii, cell-cell interactions and progression of the sexual cycle are similar for many homothallic and heterothallic species of the genus. Regulation of chloroplast gene transmission by the nuclear mating-type alleles (mt+ and mt-) is another common denominator for Chlamydomonas species studied thus far. We have previously reported the use of chloroplast inheritance patterns to identify mutants of C. monoica that have lost the potential to function as the mt+ mating-type. A similar screening procedure led to the isolation of an unusual mutant, mtl-3 whose phenotype is less readily explained. Chloroplast gene transmission patterns in crosses involving mtl-3 suggest that the mtl-3 strain mates preferentially as mt+. However, normal mating efficiencies and high zygospore viability are observed in clonal culture, ...
Read UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
In 1933, Marjory Stephenson and her student Stickland reported that cell suspensions catalysed the reduction of methylene blue with H2. Six years later, Hans Gaffron observed that the green photosynthetic alga Chlamydomonas reinhardtii, would sometimes produce hydrogen.[17] In the late 1990s Anastasios Melis discovered that deprivation of sulfur induces the alga to switch from the production of oxygen (normal photosynthesis) to the production of hydrogen. He found that the enzyme responsible for this reaction is hydrogenase, but that the hydrogenase lost this function in the presence of oxygen. Melis also discovered that depleting the amount of sulfur available to the algae interrupted their internal oxygen flow, allowing the hydrogenase an environment in which it can react, causing the algae to produce hydrogen.[18] Chlamydomonas moewusii is also a promising strain for the production of hydrogen.[19][20] ...
Definition of chlamydomonas - a common single-celled green alga which typically has two flagella for swimming, living in water and moist soil.
Anti-Lhcb4 (CP29) Chlamydomonas reinhardti, Lhcb4, CP29, Lhcbm4 antibody , Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas, Q93WD2, AS06 117
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Algae Detail UTEX Number: 943Class: ChlorophyceaeStrain: Chlamydomonas giganteaMedia: Modified Bold 3N MediumOrigin: Description of Location: Type Culture: NoCo
Algae Detail UTEX Number: 969Class: ChlorophyceaeStrain: Chlamydomonas applanataMedia: Modified Bold 3N MediumOrigin: Williamson Co., Texas, USADescription of L
Immunofluorescence micrograph of a Chlamydomonas cell stained with acetylated tubulin antibody to label flagella (green) and with an antibody against the protein EB1 that labels the flagella tip and base (red/yellow). Image from Lotte Pedersen, University of Copenhagen. To previous page ...
Introduction Bio 2010 01/31/2014 The objectives of this lab were to understand natural selection and understand the biotic and abiotic characteristics of a niche. In order to understand natural selection in this lab we observed characteristics of three different algae families; Volvox, Chlamydomonas, and Gonium. By observing these algae will give us a better understanding of how a simple cellular organism evolved due to natural selection and are able to survive today. These organisms will give us context into natural selection occurring on bigger organisms as we move on in lab. Similarly, in order to understand biotic and abiotic characteristics of a niche, we observed a transect. By observing a transect will provide insight into how the biosphere works by focussing on biotic and biotic components of a home to many organisms. Procedure 1: The Volvicine Line In this experiment we observed an isogamous, single celled, motile alga called chlamydomonas. We prepared a slide of a living Chlamydomonas ...
Figure 5A. Wildtype and roc15 mutant Chlamydomonas was entrained to a 12:12 light:dark cycle. The cultures were transferred to constant darkness and their tufA expression was measured with luminescence. They were then expressed to 5 minute long pulses of light. The wild type Chlamydomonas responded to the light pulses, evident by a phase shift in tufA expression. The roc15 mutant Chlamydomonas did not exhibit this phase shift. This indicate that roc15 is essential for circadian regulated phase shifts and entrainment. ...
View Notes - 10. [R] nlm = non linear minimization (Jan24) from AM 1234 at UWO. model.fit Page 1 of 1 25/01/2011 http://www.stats.uwo.ca/faculty/kulperger/Stat3858/Computing/RScripts/nlm-eg-exp.txt
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Alphabetic Listing of Presenting Authors - R. If you have any questions or comments then please send an email to [email protected] ...
pep:novel chromosome:VEGA66:10:33905485:33915883:1 gene:OTTMUSG00000029742 transcript:OTTMUST00000073807 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Rsph4a description:radial spoke head 4 homolog A (Chlamydomonas ...
Bukeye umukobwa numuhungu, mu masaha yigicamunsi barahura ngo batembere, sinzi uko umuhungu yaje kurabukwa igishyimbo mu menyo yumukobwa, umuhungu aba uwo munsi yari afite twa duti dukura ibiryo mu menyo, amuha kamwe agira ati Akira ukure ifiriti mu menyo. ...
The nuclear genome of the model organism Chlamydomonas reinhardtii contains genes for a dozen hemoglobins of the truncated lineage. Of those, THB1 is known to be expressed, but the product and its function have not yet been characterized. We present mutagenesis, optical, and nuclear magnetic resonance data for the recombinant protein and show that at pH near neutral in the absence of added ligand, THB1 coordinates the heme iron with the canonical proximal histidine and a distal lysine. In the cyanomet state, THB1 is structurally similar to other known truncated hemoglobins, particularly the heme domain of Chlamydomonas eugametos LI637, a light-induced chloroplastic hemoglobin. Recombinant THB1 is capable of binding nitric oxide (NO(*)) in either the ferric or ferrous state and has efficient NO(*) dioxygenase activity. By using different C. reinhardtii strains and growth conditions, we demonstrate that the expression of THB1 is under the control of the NIT2 regulatory gene and that the hemoglobin is
RNA pyrophosphohydrolase (RppH) catalyzes the removal of pyrophosphate from 5 triphosphorylated RNAs thereby initiating RNA degradation. The enzyme has originally been identified in bacteria but homologs are present in eukaryotes where they are thought to be located in plastids or mitochondria. A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. The purpose of this project was to determine the localization of the RppH homologue in C. reinhardtii. Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5rppH-GFP construct.. A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. PCR, RT-PCR, sequencing, and DNA and RNA blotting techniques were used to indentify positive transformants at the DNA ...
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells ...
TY - JOUR. T1 - The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii. AU - Ozawa, Shin Ichiro. AU - Cavaiuolo, Marina. AU - Jarrige, Domitille. AU - Kuras, Richard. AU - Rutgers, Mark. AU - Eberhard, Stephan. AU - Drapier, Dominique. AU - Wollman, Francis André. AU - Choquet, Yves. PY - 2020/4/1. Y1 - 2020/4/1. N2 - In the green alga Chlamydomonas (Chlamydomonas reinhardtii), chloroplast gene expression is tightly regulated posttranscriptionally by gene-specific trans-acting protein factors. Here, we report the identification of the octotricopeptide repeat protein MTHI1, which is critical for the biogenesis of chloroplast ATP synthase oligomycin-sensitive chloroplast coupling factor. Unlike most trans-acting factors characterized so far in Chlamydomonas, which control the expression of a single gene, MTHI1 targets two distinct transcripts: it is required for the accumulation and translation of atpH mRNA, encoding a subunit of ...
During intraflagellar transport (IFT), the regulation of motor proteins, the loading and unloading of cargo and the turnover of flagellar proteins all occur at the flagellar tip. To begin an analysis of the protein composition of the flagellar tip, we used difference gel electrophoresis to compare long versus short (i.e., regenerating) flagella. The concentration of tip proteins should be higher relative to that of tubulin (which is constant per unit length of the flagellum) in short compared with long flagella. One protein we have identified is the cobalamin-independent form of methionine synthase (MetE). Antibodies to MetE label flagella in a punctate pattern reminiscent of IFT particle staining, and immunoblot analysis reveals that the amount of MetE in flagella is low in full-length flagella, increased in regenerating flagella, and highest in resorbing flagella. Four methylated proteins have been identified in resorbing flagella, using antibodies specific for asymmetrically dimethylated ...
We present a new Chlamydomonas reinhardtii flagellar mutant in which central pair projections are missing and the central pair microtubules are twisted along the length of the flagellum. We have named this mutant tcp1 for twisted central pair. Immunoblots using an antibody that recognizes the heavy chain of sea urchin kinesin reveal that a 70 kDa protein present in wild-type and pf18 (central pairless) axonemes is absent in tcp1, suggesting the presence of an uncharacterized kinesin associated with the central pair apparatus. We demonstrate that the kinesin-like protein Klp1 is not attached to central pair microtubules in tcp1, but rather is located in, or is part of, a region we have termed the internal axonemal matrix. It is proposed that this matrix acts as a scaffold for axonemal proteins that may also be associated with the central pair apparatus.. ...
We report here on the UV-induced vanadate-dependent cleavage of the alpha and beta heavy chains of the outer arm dynein from Chlamydomonas flagella. Both polypeptides are cleaved at a single site (termed the V1 site) by UV irradiation in the presence of Mg2+, ATP, and vanadate. The alpha chain yields fragments of Mr 290,000 and 190,000. Fragments of Mr 255,000 and 185,000 are obtained from the beta chain. Ultraviolet irradiation of the alpha and beta chains in the presence of vanadate and Mn2+ (but no nucleotide) induces cleavage of both molecules at sites (termed the V2 sites) distinct from the V1 sites. The single V2 site within the beta chain is located 75,000 daltons from the site of V1 cleavage within the Mr 255,000 V1 fragment. The alpha chain contains three distinct sites of V2 cleavage; all are located within the Mr 290,000 V1 fragment, 60,000, 90,000, and 100,000 daltons from the site of V1 cleavage. From these studies, we estimate the masses of the alpha and beta heavy chains to be 480,000 and
The Chlamydomonas Gopher Server at Duke University offers the following files: A description of the Chlamydomonas Genetics Center Catalogue of Chlamydomonas strains available from the Center List of plasmids available from the Center List of genes that have been sequenced from Chlamydomonas species also from Volvox and Dunaliella Recent literature citations Addresses of colleagues Nuclear genetic maps, in postscript and Macintosh bin-hex formats Chloroplast and mitochondrial genetic/physical maps, same formats Methods files This server can be accessed through the Duke University Computer ASSIST Center Gopher, who have kindly provided the following access information: Name=Duke University Computer ASSIST Center Type=1 Port=70 Path= Host=gopher.duke.edu If you are not running a gopher server, you can connect by pointing your gopher client to gopher.duke.edu, port 70. If you do not have access to a gopher client, gopher client and server software is available via anonymous ftp from ...
The radial spoke is known to play a role in the mechanical movement of the flagellum/cilium. For example, mutant organisms lacking properly functioning radial spokes have flagella and cilia that are immotile. Radial spokes also influence the cilium waveform; that is, the exact bending pattern the cilium repeats. How the radial spoke carries out this function is poorly understood. Radial spokes are believed to interact with both the central pair microtubules and the dynein arms, perhaps in a way that maintains the rhythmic activation of the dynein motors. For example, one of the radial spoke subunits, RSP3, is an anchor protein predicted to hold another protein called protein kinase A (PKA). PKA would theoretically then be able to activate/inactivate the adjacent dynein arms via its kinase activity. However, the identities and functions of the many radial spoke subunits are just beginning to be elucidated. ...
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis ...
The green alga Chlamydomonas reinhardtii possesses a CO2 concentratingmechanism (CCM) which helps in successful acclimationto low CO2 conditions. Current models of the CCM postulate that aseries of ion transporters bring HCO3- from outside the cell to thethylakoid lumen, where the carbonic anhydrase CAH3 dehydratesaccumulated HCO3- to CO2, raising the CO2 concentration forRubisco. Previously, HCO3- transporters have been identified atboth the plasma membrane and the chloroplast envelope, butthe transporter thought to be on the thylakoid membrane hasnot been identified. Three paralogous genes (BST1, BST2, BST3)belonging to the bestrophin family have been found to be upregulatedin low CO2 conditions, and their expression is controlledby CIA5, a transcription factor that controls many CCM genes.YFP fusions demonstrate that all three proteins are located onthe thylakoid membrane, and interactome studies indicate thatthey might associate with chloroplast CCM components. A singlemutant defective in ...
Fusion of green fluorescent protein (GFP) to proteins is a powerful method to investigate dynamic processes in vivo. The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. In this work the GFP-tagging of proteins was employed in order to analyse proteins of the flagellar basal apparatus. Striated fiber assembling (SFA), centrin and deflagellation induced protein of 13 kDa (DIP13) were tagged with GFP at the C-terminal domain. In addition SFA was tagged at the N-terminal domain. The chimeric genes were stably transformed in C. reinhardtii. SFA is the mayor component of the striated microtubule associated fibers (SMAFS). GFP tagged SFA was incorporated into this fibers. N-terminal tagged SFA had similar properties like the wild-type protein. The length of the fibers increased with the strength of expression. The head domain of SFA is essential for fiber formation and photobleaching experiments did not show a pronounced dynamic of the fibers. The ...
View Stock Photo of The Green Algae Chlamydomonas With Its Two Flagella Sem X6000. Find premium, high-resolution photos at Getty Images.
The maintenance of flagellar length is thought to require both anterograde and retrograde intraflagellar transport (IFT). particles. The IFT particles then associate into linear arrays known as IFT trains (Pigino et al., 2009), which move processively from the base of the flagellum out to the tip. This anterograde transport Mouse monoclonal to CD74(PE) is driven by kinesin-2, a heterotrimeric complex composed of the FLA10 and FLA8 motor subunits (Walther et al., 1994) and the buy BS-181 HCl kinesin-associated protein KAP (Cole et al., 1993; Mueller et al., 2005). After their anterograde motion to the flagellar tip, IFT particles rearrange into a new set of IFT trains that move back to the base of the flagellum. This retrograde transport is powered by cytoplasmic dynein 1b, a large complex composed of the heavy chain motor subunit DHC1b (Pazour et al., 1999a; Porter et al., 1999; Signor et al., 1999) and numerous smaller components including D1bLIC (Perrone et al., 2003; Schafer et al., 2003; Hou ...
Catalytic domain of the Protein Serine/Threonine Kinase, Chlamydomonas reinhardtii FA2 and similar domains. Serine/Threonine Kinases (STKs), Chlamydomonas reinhardtii FA2-like subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Chlamydomonas reinhardtii FA2-like subfamily belongs to the (NIMA)-related kinase (Nek) family. The Nek family includes seven different Chlamydomonas Neks (CNKs 1-6 and Fa2). This subfamily includes FA2 and CNK4. The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Chlamydomonas reinhardtii FA2 was discovered in a genetic screen for deflagellation-defective mutants. It is essential for basal-body/centriole-associated microtubule severing, and plays a role in cell cycle progression. No cellular ...
Our initial mapping data placed Mcd4 into two possible locations. In both cases, it was desirable to generate bacterial artificial chromosome (BAC) contigs for eventual complementation, as well as additional markers. As a case study, we describe how BAC contigs can be extended using Chlamydomonas resources and our experience in generating site-specific markers.. Several BAC libraries have been constructed for Chlamydomonas, two of which are available through the Clemson Genomics Institute (http://www.genome.clemson.edu/groups/bac/). In addition, BAC contigs (http://www.biology.duke.edu/chlamy_genome/BAC/index.html) have been assembled for most of the STS and RFLP markers. In the case of mcd4, Gsp1 resides on scaffold 2 and CNA45 on scaffold 66. A 41-BAC contig exists for scaffold 2 covering approximately 1,000 kb (R. Nguyen, personal communication), and a smaller contig is linked to scaffold 66 and CNA45 (Fig. 6). An unknown amount of DNA separates scaffolds 2 and 66; such discontinuities in the ...
Buy RSPH4A elisa kit, Human radial spoke head 4 homolog A (Chlamydomonas) ELISA Kit-NP_001010892.1 (MBS9339167) product datasheet at MyBioSource, ELISA Kits
Molecular motors in living cells are involved in whole-cell locomotion, contractility, developmental shape changes, and organelle movement and positioning. Whether motors of different directionality are functionally coordinated in cells or operate in a semirandom tug of war is unclear. We show here that anterograde and retrograde microtubule-based motors in the flagella of Chlamydomonas are regulated such that only motors of a common directionality are engaged at any single time. A laser trap was used to position microspheres on the plasma membrane of immobilized paralyzed Chlamydomonas flagella. The anterograde and retrograde movements of the microsphere were measured with nanometer resolution as microtubule-based motors engaged the transmembrane protein FMG-1. An average of 10 motors acted to move the microsphere in either direction. Reversal of direction during a transport event was uncommon, and quiescent periods separated every transport event, suggesting the coordinated and exclusive ...
Chlamydomonas PsbK protein: required for the stability of the photosystem II complex and essential for photoautotrophic growth; stability requirements of the photosynthetic complexes differ between cyanobacteria and C. reinhardtii; MW 4 kDa; has been sequenced
Single-cell alga Chlamydomonas, are able to generate cellular energy from the breakdown of sugars without taking up oxygen. They do this using a variety...
Probable component of the axonemal radial spoke head (By similarity). Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk, which is attached to a subfiber of the outer doublet microtubule, and a bulbous head, which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
This model does not account for two published observations: An mt+ strain carrying the MID gene transposed to an autosome differentiates as minus, as do mt+ cells transformed with the MID gene, even though neither possesses a copy of the MTD1 gene (Ferris and Goodenough 1997). To reconcile these observations with the results reported here, we are led to propose that plus gametes express a system, the MTD1-equivalent system, that is functionally equivalent to the MTD1 system but achieves this outcome without requiring the Mtd1 protein itself. When MID is introduced into a plus background, the MTD1-equivalent system enables sufficiently high MID expression to allow transformants to undergo minus differentiation, albeit success is usually incomplete (see results and Ferris and Goodenough 1997), meaning that the MTD1-equivalent system is not repressible by Mid. Importantly, at least one essential gene in the posited plus MTD-equivalent system must be resident in the MT+ locus. If the system were ...
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When mating type plus and minus gametes of Chlamydomonas are mixed, they agglutinate with each other via their flagella, fuse, then initiate the zygote formation program which includes synthesis of the zygote cell wall, fusion of nuclei and chloroplasts, and the digestion of chloroplast DNA from the minus parent. The mRNAs from gamete and zygote cells was isolated and hybridized to cDNA-macroarray filters both to identify new genes expressed during the mating reaction and the early zygote formation process and to analyze the gene expression programs that underlie these sexual processes. Twenty-one novel genes were identified in this screen, designated as EZY (early zygote expressed) genes. The EZY genes included genes encoding proteins whose function is unknown, and genes encoding proteins that appear to be involved in processes such as cell wall synthesis, gene expression, intracellular trafficking or secretion, and vesicular transport in zygotic cells. All of the EZY genes were strongly ...
2) Cre06.g293900, chromosome_6 base 6636206 (genome v5.5), intron - position has 95% probability of being correct. The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.. ...
The green algae Chlamydomonas snowiae is named after her, as is the bacterial Genus Snowella. Her older brother, Dr. Benjamin ... Guiry, M. D. (April 11, 2002). "Chlamydomonas snowiae Printz". AlgaeBase. National University of Ireland, Galway. Retrieved ...
Euglena and Chlamydomonas). This flagella-associated structure observed in the large selenomonad can perhaps best be described ...
Examples: Chlamydomonas ("cloak unit"); Pseudomonas ("false unit"); Metamonad ("encompassing unit") -morph: Pronunciation: / ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-7. doi:10.1104/pp.010807. PMC 1540183. PMID 11743094. ...
Lessons from Chlamydomonas reinhardtii". Plant Physiology. 127 (4): 1500-1507. doi:10.1104/pp.010807. PMC 1540183. PMID ...
"Chlamydomonas Ehrenberg, 1833: 288". algaeBASE. Retrieved 19 May 2013. "Spirogyra Link, 1820: 5". algaeBASE. Retrieved 19 May ... Rochaix JD (1998). The molecular biology of chloroplasts and mitochondria in Chlamydomonas. Dordrecht [u.a.]: Kluwer Acad. Publ ... Chlamydomonas), a ribbon-like spiral around the edges of the cell (e.g., Spirogyra), or slightly twisted bands at the cell ... required for normal pyrenoid formation in Chlamydomonas reinhardtii". Plant Physiology. 156 (2): 884-96. doi:10.1104/pp. ...
Crutchfield A, Diller K, Brand J (1999-02-01). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal ...
Chlamydomonas reinhardtii and Volvox carteri). The latter implies that UVR8 potentially appeared before the evolutionary split ...
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
This location is one of the most southerly locations where red snow algae (Chlamydomonas sp., Chloromonas sp., and ... Chlamydomonas nivalis) are found. The location is favourable for vegetation growth because of warm summer temperatures. Its ...
Chlamydomonas, Eudorina or Oogamous e.g. Chlamydomonas, Volvox.[clarification needed] Chlamydomonas has all three types of ... The chloroplast may be discoid, cup-shaped (e.g. Chlamydomonas), spiral or ribbon shaped (e.g. Spirogyra) Most chlorophytes ... The following orders are typically recognised: Dunaliellales (e.g. Dunaliella) Chlamydomonadales (e.g. Volvox, Chlamydomonas) ...
Chlamydomonas reinhardtii) and archaea (e.g., Methanococcus jannaschii). The proteins are of about 450 amino acyl residues in ...
Harz H, Hegemann P (1991-06-06). "Rhodopsin-regulated calcium currents in Chlamydomonas". Nature. 351 (6326): 489-491. Bibcode: ... they could demonstrate that a single gene from the alga Chlamydomonas produced large photocurrents when expressed in the oocyte ...
Johnson KA, Rosenbaum JL (December 1992). "Polarity of flagellar assembly in Chlamydomonas". The Journal of Cell Biology. 119 ( ... melanogaster and Chlamydomonas reinhardtii. In mammals, disruption of the transition zone reduces the ciliary abundance of ...
Photocurrents of the Chlorophyceae Heamatococcus pluvialis and Chlamydomonas reinhardtii were studied over many years in the ... Harz H, Hegemann P (June 1991). "Rhodopsin-regulated calcium currents in Chlamydomonas". Nature. 351 (6326): 489-491. doi: ... Kateriya, S. Fuhrmann, M. Hegemann, P.: Direct Submission: Chlamydomonas reinhardtii retinal binding protein (cop4) gene; ... and high-intensity light in Chlamydomonas reinhardtii". Proc. Natl. Acad. Sci. U.S.A. 99 (13): 8689-94. doi:10.1073/pnas. ...
"Radial spoke proteins of Chlamydomonas flagella". Journal of Cell Science. 119 (Pt 6): 1165-74. doi:10.1242/jcs.02811. PMC ...
based on the Chlamydomonas reinhardtii genome. It has 866 unique ORFs, 1862 metabolites, 2499 gene-enzyme-reaction-association ... a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome". BMC Genomics. 12 Suppl 4: S5. ...
Ensuing studies 20 years after the identification of the same mutant strain of Chlamydomonas reinhardtii found that the ... When a photorespiratory mutant of the eukaryotic green alga Chlamydomonas reinhardtii was studied, the mutant strain was ... Suzuki K, Marek LF, Spalding MH (May 1990). "A Photorespiratory Mutant of Chlamydomonas reinhardtii". Plant Physiology. 93 (1 ... "Transcriptional Analysis of the Three Phosphoglycolate Phosphatase Genes in Wild Type and the pgp1 Mutant of Chlamydomonas ...
Staub M, Denes G (1966). "Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. I Purification and properties of ...
Cavalier-Smith, Thomas (1967). Organelle development in Chlamydomonas reinhardii (PhD thesis). University of London. OCLC ... "Organelle Development in Chlamydomonas reinhardii". From 1967 to 1969, he was a guest investigator at Rockefeller University. ...
Through her research using the single-celled ciliated green alga Chlamydomonas reinhardtii as a model organism, Quarmby ... "The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during ... "Genetics of the deflagellation pathway in Chlamydomonas". Genetics. 149 (2): 927-936. PMC 1460167. PMID 9611203. Mahjoub, Moe R ...
The unicellular green alga, Chlamydomonas reinhardtii grows as vegetative cells in nutrient rich growth medium, but depletion ... Sager R, Granick S (July 1954). "Nutritional control of sexuality in Chlamydomonas reinhardi". The Journal of General ...
Chlamydomonas reinhardtii, a unicellular relative of V. carteri, is known to possess genes related to regA. This suggests that ... V. carteri forms small spherical colonies, or coenobia, of 2000-6000 Chlamydomonas-type somatic cells and 12-16 large, ... Miller, Stephen M. (2010). "Volvox, Chlamydomonas, and the Evolution of Multicellularity". Nature Education. 3 (9): 65. ...
Schimmer, O; Kühne, I (1991). "Furoquinoline alkaloids as photosensitizers in Chlamydomonas reinhardtii". Mutation Research. ...
"Entrez Gene: radial spoke 3 homolog (Chlamydomonas)". CS1 maint: discouraged parameter (link) Koukoulas I, Augustine C, ...
"Entrez Gene: intraflagellar transport 81 homolog (Chlamydomonas)". Higashi M, Kobayashi K, Iijima M, Wakana S, Horiuchi M, ...
S. M. Miller (2010). "Volvox, Chlamydomonas, and the evolution of multicellularity". Nature Education. 3 (9): 65. Brian Keith ...
She worked alongside Bruce Selman on the single-cell algae Chlamydomonas reinhardtii. Chlamydomonas reinhardtii is a model ... She showed that chlamydomonas also contained cytochrome c6, which could step in during photosynthesis if needed. In 1987 ... In 2007 she led a team that sequenced the Chlamydomonas genome. Her inaugural article was published in PNAS in 2009. She spent ... Merchant was the first to demonstrate that the RNA for Chlamydomonas reinhardtii plastocyanin is produced when copper is ...
Although he has worked with numerous model organisms, he is best known for his work with Chlamydomonas reinhardtii (C. ... Davies, J., Yildiz F., Grossman, A.R. (1996) Sac1, a putative regulator that is critical of survival of Chlamydomonas ... 2014-10-01). "The Chlamydomonas genome project: a decade on". Trends in Plant Science. 19 (10): 672-680. doi:10.1016/j.tplants. ... Aksoy, M., Pootakham, W., Grossman, A.R. (2014) Critical function of a Chlamydomonas reinhardtii putative vacuolar transporter ...
Farago A, Denes G (1967). "Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. II Purification and properties of N- ...
Chlamydomonas reinhardtii Transcription Factor Database. *"Chlamydomonas", a song by Andy Offutt Irwin about the life cycle of ... Chlamydomonas reinhardtii[9]. Ecology[edit]. Widely distributed in freshwater or damp soil.[1]It is generally found in habitat ... Chlamydomonas is a genus of green algae consisting of about 325 species[1] all unicellular flagellates, found in stagnant water ... Chlamydomonass asexual reproduction occurs by zoospores, by aplanospores, by hypnospores or by a palmella stage;[10] sexual ...
Chlamydomonas species are widely distributed worldwide in soil and fresh water. Chlamydomonas reinhardtii is an especially well ... "Home - Chlamydomonas reinhardtii v3.0". "Chlamydomonas reinhardtii mitochondrion, complete genome". February 2010. Cite journal ... "Chlamydomonas reinhardtii". AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Chlamydomonas ... Chlamydomonas is used as a model organism for research on fundamental questions in cell and molecular biology such as: How do ...
... Dr. Morgan L. Vis vis-chia at oak.cats.ohiou.edu Sat Mar 9 19:19:19 EST 2002 *Previous message: ... The 10th International Conference on the Cell and Molecular Biology of Chlamydomonas will be held in Vancouver, Canada, June 11 ... Elizabeth Harris chlamy at duke.edu Chlamydomonas Genetics Center home page: http://www.biology.duke.edu/chlamy/ Resource ...
We propose to study complex I assembly in the single-celled green alga Chlamydomonas reinhardtii. Chlamydomonas mutants lacking ... Chlamydomonas] research position. Patrice Hamel via chlamy%40net.bio.net (by hamel.16 from osu.edu). Sun Aug 3 18:20:50 EST ... for instance) to study mitochondrial complex I assembly in the green alga Chlamydomonas (see below for a scientific summary of ...
... Sara Cline via chlamy%40net.bio.net (by cline.228 from osu.edu). Thu May 5 18:31:52 EST 2011 * ... Hello, I was wondering if anyone had ever performed a Dot Blot of Chlamydomonas whole cell extract directly on PVDF or ...
... Josh Rappoport rappopj at mail.rockefeller.edu Tue Jul 19 12:10:46 EST 2005 * Messages sorted ...
High-frequency nuclear transformation of Chlamydomonas reinhardtii.. K L Kindle. PNAS February 1, 1990 87 (3) 1228-1232; https ... PF15p Is the Chlamydomonas Homologue of the Katanin p80 Subunit and Is Required for Assembly of Flagellar Central Microtubules ... Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division ... Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly ...
GENETIC CONTROL OF PHOTOSYNTHESIS IN CHLAMYDOMONAS REINHARDI. R. P. Levine. PNAS July 1, 1960 46 (7) 972-978; https://doi.org/ ...
The Chlamydomonas cell cycle.. Cross FR1, Umen JG.. Author information. 1. The Rockefeller University, New York, NY, 10065, USA ... The position of Chlamydomonas within the eukaryotic phylogeny makes it a unique model in at least two important ways: as a ... Chlamydomonas multiple‐fission cell cycle. Schematic from left to right of one multiple‐fission cycle. Daughter cells grow ... The Chlamydomonas cell cycle has a striking temporal and functional separation between cell growth and rapid cell division, ...
Purchase The Chlamydomonas Sourcebook: Organellar and Metabolic Processes - 2nd Edition. Print Book & E-Book. ISBN ... Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, ... This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in ... and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism ...
Chlamydomonas reinhardtii. Mutation(s): 0 Gene Names: rbcL. EC: Find proteins for P00877 (Chlamydomonas reinhardtii) ... Chlamydomonas reinhardtii, Spinacia oleracea. Mutation(s): 0 Gene Names: RBCS-1, CHLRE_02g120100v5, CHLREDRAFT_82986. EC: 4.1. ... In the present study, chimeric small subunits were constructed by replacing the loop of the green alga Chlamydomonas ... When these engineered genes were transformed into a Chlamydomonas mutant that lacks small-subunit genes, photosynthesis- ...
1984 Effects of cell division on the stigma of wild-type and an "eyeless" mutant of Chlamydomonas. J. Ultrastruct. Res. 87: 46- ... Eyespot-Assembly Mutants in Chlamydomonas reinhardtii. Mary Rose Lamb, Susan K. Dutcher, Cathy K. Worley and Carol L. Dieckmann ... 1992 The photoreceptor current of the green alga Chlamydomonas. Philos. Trans. R. Soc. Lond. B Biol. Sci. 338: 38-52. ... Eyespot-Assembly Mutants in Chlamydomonas reinhardtii. Mary Rose Lamb, Susan K. Dutcher, Cathy K. Worley and Carol L. Dieckmann ...
New directions in Chlamydomonas biology. B. Select a presentation type: oral or poster.. Step 3. Enter all the author names and ... Correct format - Sample abstract title for the Chlamydomonas Conference Incorrect format - INCORRECT STYLE FOR THE ABSTRACT ... Chlamydomonas Conference abstracts and/or other compilations or collective works to be assembled and owned by GSA (together, ...
Chlamydomonas reinhardtii. Mutation(s): 2 Gene Names: rbcL. EC: Find proteins for P00877 (Chlamydomonas reinhardtii) ... Chlamydomonas reinhardtii. Mutation(s): 0 Gene Names: RBCS-1, CHLRE_02g120100v5, CHLREDRAFT_82986. EC: ... 5-bisphosphate carboxylase/oxygenase from the unicellular green alga Chlamydomonas reinhardtii causes a 13% decrease in CO(2)/O ... 5-bisphosphate carboxylase/oxygenase from the unicellular green alga Chlamydomonas reinhardtii causes a 13% decrease in CO(2)/O ...
Shrager, J, Hauser, C, Chang, CW, Harris, EH, Davies, J, McDermott, J, Tamse, R, Zhang, Z, Grossman, AR 2003Chlamydomonas ... Maul, JE, Lilly, JW, Cui, L, DePamphilis, CW, Miller, W, Harris, EH, Stern, DB 2002The Chlamydomonas reinhardtii Plastid ... Boudreau, E, Nickelsen, J, Lemaire, SD, Ossenbuhl, F, Rochaix, JD 2000The Nac2 gene of Chlamydomonas encodes a chloroplast TPR- ... Klein, U, De Camp, JD, Bogorad, L 1992Two types of chloroplast gene promoters in Chlamydomonas reinhardtiiProc Natl Acad Sci ...
Tryptophan analog resistance mutations in Chlamydomonas reinhardtii.. S K Dutcher, R E Galloway, W R Barclay and G Poortinga ... Tryptophan analog resistance mutations in Chlamydomonas reinhardtii.. S K Dutcher, R E Galloway, W R Barclay and G Poortinga ... Tryptophan analog resistance mutations in Chlamydomonas reinhardtii.. S K Dutcher, R E Galloway, W R Barclay and G Poortinga ... Tryptophan analog resistance mutations in Chlamydomonas reinhardtii. Message Subject (Your Name) has forwarded a page to you ...
The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the ... Identification of nitrate transporter genes in Chlamydomonas reinhardtii Plant J. 1994 Mar;5(3):407-19. doi: 10.1111/j.1365- ... The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the ...
The type species (holotype) of the genus Chlamydomonas is Chlamydomonas reinhardtii P.A.Dangeard. ... Chlamydomonas spirotaenia Pascher. Classification: Empire Eukaryota. Kingdom Plantae. Subkingdom Viridiplantae. Infrakingdom ... Chlamydomonas spirotaenia Pascher 1930: 138, fig. 33. Published in: Pascher, A. (1930). Neue Volvocalen (Polyblepharidinen- ...
The type species (holotype) of the genus Chlamydomonas is Chlamydomonas reinhardtii P.A.Dangeard. ... Chlamydomonas clathrata Pascher. Classification: Empire Eukaryota. Kingdom Plantae. Subkingdom Viridiplantae. Infrakingdom ... Chlamydomonas clathrata Pascher 1927: 192 (key), 305, fig. 274b. Published in: Pascher, A. (1927). Volvocales = Phytomonadinae ... Pröschold, T., Marin, B., Schlösser, U.W. & Melkonian, M. (2001). Molecular phylogeny and taxonomic revision of Chlamydomonas ( ...
Chlamydomonas debaryana strain NIES-2212, whole genome shotgun sequencing projec... Chlamydomonas debaryana strain NIES-2212, ... Chlamydomonas debaryana strain NIES-2212, whole genome shotgun sequencing project. GenBank: BDDB00000000.1 ...
Francesca Zito (CNRS, France) for providing the Chlamydomonas strain and her insights on Chlamydomonas growth and physiology. ... Blaby IK et al (2014) The Chlamydomonas genome project: a decade on. Trends Plant Sci 19:672-680CrossRefGoogle Scholar ... Boyle NR, Morgan JA (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3:4CrossRef ... Merchant SS et al (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318:245-250 ...
... Just in time for holiday shopping, Chytridoodles has a whole line of gear illustrated with a Chlamydomonas ... New #Chlamydomonas print from @chytridoodles! #Chlamy is an example of pond #algae and a model organism for the biology of ... Advertising Algae Alternative medicine Art Bacteria Blog Books Cdesign proponentsists Cellular differentiation Chlamydomonas ...
Chlamydomonas reinhardi (+) Optimal Medium: Soil Extract Agar Optimal Temperature: 22° C Optimal Light: High (200 to 400 foot- ... Chlamydomonas reinhardi (+), Bacteria-Free, Living. Item # 152040 Chlamydomonas reinhardi+, Bacteria-Free, Living is rated 5.0 ... Chlamydomonas reinhardi (+) is a wild-type bacteria-free algae culture that crosses with Chlamydomonas reinhardi (-) (item # ... Genus and Species: Chlamydomonas reinhardi (+). Optimal Medium: Soil Extract Agar. Optimal Temperature: 22° C. Optimal Light: ...
Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi ... The Chlamydomonas cell wall and its constituent glycoproteins analyzed by the quick-freeze, deep-etch technique. *Goodenough U ... Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi ...
Chlamydomonas reinhardtii (Chlamydomonas smithii). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand% ... "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.". Piperno G., ... "Three distinct inner dynein arms in Chlamydomonas flagella: molecular composition and location in the axoneme.". Piperno G., ... sp,B5BUZ8,KTU_CHLRE Protein kintoun OS=Chlamydomonas reinhardtii OX=3055 GN=pf13 PE=2 SV=1 ...
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions journal, October 2007 * Merchant, S. S.; ... Accepted Manuscript: Genomics and functional genomics in Chlamydomonas reinhardtii. Title: Genomics and functional genomics in ... Here, in this paper, we employ the reference unicellular green alga Chlamydomonas reinhardtii to identify the effect of H 2O 2 ... Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing ...
The GeneArt Chlamydomonas protein expression products, a eukaryotic protein expression system, simplifies metabolic engineering ... Enhanced transfection efficiency for Chlamydomonas One of the biggest hurdles in research and development with Chlamydomonas ... enhances transformation efficiency for multiple strains of Chlamydomonas. It increases permeability of the Chlamydomonas cell ... Gibco TAP Growth Media-optimized for Chlamydomonas Gibco TAP Growth Medium, offered separately, is optimized for the growth and ...
Regulation of CCM genes in Chlamydomonas .... POSTER. Light, carbon, and other environmental responses. 150. Monday, June 9. ... New directions in Chlamydomonas biology. 70. Wednesday, June 11. Merrill Hall. 3:15 pm. ...
  • We propose to study complex I assembly in the single-celled green alga Chlamydomonas reinhardtii. (bio.net)
  • In the present study, chimeric small subunits were constructed by replacing the loop of the green alga Chlamydomonas reinhardtii with the sequences of Synechococcus or spinach. (rcsb.org)
  • Substitution of Leu290 by Phe (L290F) in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from the unicellular green alga Chlamydomonas reinhardtii causes a 13% decrease in CO(2)/O(2) specificity and reduced thermal stability. (rcsb.org)
  • The biflagellate green alga Chlamydomonas reinhardtii exhibits both positive and negative phototaxis to inhabit areas with proper light conditions. (nih.gov)
  • The versatile metabolism of the green alga Chlamydomonas reinhardtii is reflected in its complex response to anaerobic conditions. (mcponline.org)
  • Cells of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. (cellimagelibrary.org)
  • We have cloned a gene ( Mut6 ) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. (sciencemag.org)
  • Sometimes called green yeast ( Goodenough, 1992 ), the unicellular, eukaryotic green alga Chlamydomonas reinhardtii (hereafter called Chlamydomonas) is a venerable model system for plant biology as well as for cell motility. (plantphysiol.org)
  • The single chloroplast of the unicellular alga Chlamydomonas reinhardtii contains an orange carotenoid pigment spot that functions in phototaxis. (cellimagelibrary.org)
  • Here we report a detailed characterization of the remodeling of photosynthesis upon sulfur starvation under heterotrophy and photo-autotrophy in the green alga ( Chlamydomonas reinhardtii ). (plantphysiol.org)
  • Isolated chloroplasts of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. (ucsd.edu)
  • The unicellular green alga Chlamydomonas reinhardtii is capable of producing H 2 by splitting water with energy from sunlight. (rsc.org)
  • A homolog of the bacterial RNA pyrophosphohydrolase is present in the unicellular green alga Chlamydomonas reinhardtii suggesting that Chlamydomonas RppH has a role in mRNA degradation in the chloroplast of the alga. (uio.no)
  • The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. (frontiersin.org)
  • 2D measurement of ion currents associated to the signal transduction of the phototactic alga Chlamydomonas reinhardtii. (biomedsearch.com)
  • For example, 5′ ends are often formed by endonucleolytic processing of primary transcripts, and this may be the exclusive mode of 5′ end formation in chloroplasts in the green alga Chlamydomonas reinhardtii (reviewed in reference 12 ). (asm.org)
  • The work describes a novel approach for sustained photobiological production of H2 gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii. (oilgae.com)
  • To produce energy for cell growth, the green alga Chlamydomonas reinhardtii possesses the metabolic flexibility to use light and/or carbon sources such as acetate. (mcponline.org)
  • The time course of and the influence of light intensity and light quality on the induction of a mitochondrial carbonic anhydrase (CA) in the unicellular green alga Chlamydomonas reinhardtii was characterized using western and northern blots. (diva-portal.org)
  • In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta CAs. (openthesis.org)
  • We used a mass spectrometry-based approach to achieve a comprehensive mapping of the in vivo protein phosphorylation sites within photosynthetic membranes from the green alga Chlamydomonas reinhardtii subjected to distinct environmental conditions known to affect the photosynthetic machinery. (diva-portal.org)
  • The first intracellular algal α‐CA (Cah3) was identified in the green alga Chlamydomonas reinhardtii ( Karlsson et al , 1998 ). (embopress.org)
  • The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology conserved throughout the eukaryotic phylogeny. (g3journal.org)
  • Mass spectrometric and co-localization studies with the model alga Chlamydomonas reinhardtii have helped identify EPYC1 as a linker molecule for Rubisco with in the pyrenoid (Mackinder et al. (cam.ac.uk)
  • By using principles of synthetic biology, we have constructed a platform to characterize regulatory properties of miRNAs in the model alga Chlamydomonas reinhardtii. (cam.ac.uk)
  • Lefebvre, Paul 2004-10-06 00:00:00 A new transposable element, Tcr3, was identified in the unicellular green alga Chlamydomonas reinhardtii. (deepdyve.com)
  • [2] Chlamydomonas is used as a model organism for molecular biology , especially studies of flagellar motility and chloroplast dynamics, biogeneses, and genetics. (wikipedia.org)
  • The availability of efficient nuclear and chloroplast transformation in Chlamydomonas provides specific advantages for the study of chloroplast biogenesis, photosynthesis, and nuclear-chloroplast genome interactions. (pnas.org)
  • Genetic and biochemical studies have revealed that chloroplast gene expression in Chlamydomonas is controlled primarily post-transcriptionally, including events that effect mRNA processing and stability, and during the translation of plastid mRNAs into proteins. (springer.com)
  • Emergence of the draft sequence of the Chlamydomonas nuclear genome has enabled us to carry out a prediction and comparative analysis of the proteins required for chloroplast mRNA translation. (springer.com)
  • Transgene expression from the Chlamydomonas nuclear genome via the pChlamy_4 vector offers several advantages over chloroplast expression, such as posttranslational modifications and protein targeting and/or secretion. (thermofisher.com)
  • Chlamydomonas has been a favored organism for studying such differing topics as the eukaryotic flagellum and basal bodies, and photosynthesis and nuclear/chloroplast genetic interactions. (genetics-gsa.org)
  • Chlamydomonas reinhardtii is a unicellular eukaryotic alga possessing a single chloroplast that is widely used as a model system for the study of photosynthetic processes. (plantcell.org)
  • The program MultiPipMaker was used to compare the genic complement of Chlamydomonas with those of other chloroplast genomes and to scan the genomes for sequence similarities and repetitive DNAs. (plantcell.org)
  • Chlamydomonas reinhardtii is an excellent model system for plant biologists because of its ease of manipulation, facile genetics, and the ability to transform the nuclear, chloroplast, and mitochondrial genomes. (plantphysiol.org)
  • Ribosomal subunits affected by antibiotic resistance mutations at seven chloroplast loci in Chlamydomonas reinhardtii . (springer.com)
  • A method for complementation analysis of nuclear and chloroplast mutants of photosynthesis in Chlamydomonas . (springer.com)
  • Genes affecting erythromycin resistance and sensitivity of Chlamydomonas reinhardi chloroplast ribosomes. (springer.com)
  • Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloropast gene transmission in Chlamydomonas . (springer.com)
  • Electron microscopic evidence for chloroplast fusion in zygotes of Chlamydomonas reinhardii . (springer.com)
  • Deletion of the Chlamydomonas chloroplast atpB 3′ IR in strain Δ26 results in reduced accumulation of atpB transcripts and the chloroplast ATPase β-subunit, leading to weakly photosynthetic growth. (asm.org)
  • however, the Chlamydomonas genome encodes presumed plant orthologs of a chloroplast lipid transporter consisting of TGD (TRIGALACTOSYLDIACYLGLYCEROL) proteins that are required for ER-tochloroplast lipid trafficking in plants. (unl.edu)
  • We hypothesize this to be a result of phosphatidic acid accumulation in the chloroplast outer envelope membrane, the location of MGDG synthase in Chlamydomonas. (unl.edu)
  • Circadian regulation of chloroplast transcription in Chlamydomonas is accompanied by little or no fluctuation in RPOD levels or core RNAP activity. (biomedsearch.com)
  • In Chlamydomonas growing under 24 h light-dark cycles, chloroplast transcription is under circadian clock control, and peaks early in the morning. (biomedsearch.com)
  • The peak (but not trough) requires ongoing cytoplasmic translation, as it is sensitive to cycloheximide (CH). The chloroplast transcriptional apparatus in Chlamydomonas is simpler than in land plants, with only one type of RNA polymerase (RNAP, bacterial) and apparently only one sigma factor (RPOD). (biomedsearch.com)
  • A ) Slice through a tomogram of the chloroplast within an intact Chlamydomonas cell. (elifesciences.org)
  • N. F. Wilson and P. A. Lefebvre, abstract presented at the 10th International Chlamydomonas Conference, 2002). (asm.org)
  • Abstract The dissolved inorganic carbon concentrating mechanism(s) of Chlamydomonas moewusii CC 55 was compared with C. reinhardtii strain 137. (semanticscholar.org)
  • The availability of the Chlamydomonas reinhardtii nuclear genome sequence continues to enable researchers to address biological questions relevant to algae, land plants and animals in unprecedented ways. (osti.gov)
  • article{osti_1351725, title = {Genomics and functional genomics in Chlamydomonas reinhardtii}, author = {Blaby, Ian K. and Blaby-Haas, Crysten E.}, abstractNote = {The availability of the Chlamydomonas reinhardtii nuclear genome sequence continues to enable researchers to address biological questions relevant to algae, land plants and animals in unprecedented ways. (osti.gov)
  • 2014) to modulate the expression of algae mRNA and protein, we tested if we can alter specific enzyme levels in Chlamydomonas reinhardtii. (scirp.org)
  • 1 Many species of cyanobacteria and eukaryotic green algae, including the model organism Chlamydomonas reinhardtii , are capable of catalyzing this reaction. (rsc.org)
  • If green algae of the species Chlamydomonas reinhardtii meet Pseudomonas protegens bacteria, their fate is sealed. (algaeindustrymagazine.com)
  • Sarah Zhang writes in Wired Magazine that the single-cell green algae Chlamydomonas reinhardtii have an eyespot that makes use of light-sensitive proteins. (algaeindustrymagazine.com)
  • Some algae like Chlamydomonas reinhardtii (or "Chlamy," as it's known to its large research community) produce energy-dense oils or lipids when stressed, and these lipids can then be converted into fuels. (algaeindustrymagazine.com)
  • AID 463189 - 96-well format Chlamydomonas reinhardtii Algae Gravitaxis Assay to measure the difference in the absorbance between the small compact plug of WT swimming algae versus the MUT algae lacking cilia. (nih.gov)
  • We have found that the model unicellular green algae Chlamydomonas reinhardtii forms aggregates in response to the presence of the filter feeding zooplanktonic predator, Daphnia magna. (k-state.edu)
  • The GAPDH, CP12 and PRK recombinant proteins are able to reconstitute spontaneously the ternary complex that has been described in Chlamydomonas reinhardtii. (sigmaaldrich.com)
  • Some regulatory systems of Chlamydomonas are more complex than their homologs in Gymnosperms , with evolutionarily related regulatory proteins being larger and containing additional domains. (wikipedia.org)
  • The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas. (wikipedia.org)
  • Chlamydomonas chloroplasts contain all of the general translation factors found in bacteria, and a majority of the ribosomal proteins are conserved between plastids and bacteria. (springer.com)
  • However, Chlamydomonas contains a number of additional proteins and protein domains associated with the plastid ribosome, while some ribosomal proteins are either quite divergent or lacking. (springer.com)
  • Chlamydomonas reinhardtii is a freshwater green microalga that is a popular model organism for physiological, molecular, biochemical, and genetic studies, and that has gained attention as a platform for the production of therapeutic proteins and vaccines. (thermofisher.com)
  • In order to circumvent the transgene silencing that often occurs in Chlamydomonas reinhardtii , our pChlamy_4 vector is designed so that proteins are expressed as transcriptional fusions with the bleomycin/Zeocin resistance gene sh-ble (Rasala et al. (thermofisher.com)
  • 3 Intraflagellar Transport 122 Homolog (Chlamydomonas) (IFT122) Proteins from 2 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
  • In this study, we established detailed two-dimensional protein maps of Chlamydomonas reinhardtii light-harvesting proteins (Lhca and Lhcb) by extensive tandem mass spectrometric analysis. (asm.org)
  • Localization of ACK and PAT Venus fusion proteins in Chlamydomonas. (algaeindustrymagazine.com)
  • In Chlamydomonas reinhardtii microtubules and associated proteins are added simultaneously. (elifesciences.org)
  • This improved efficiency should greatly facilitate a variety of genetic and cell-biological studies in Chlamydomonas and also enable new applications such as expression-based screens and large-scale production of foreign proteins. (g3journal.org)
  • These problems have handicapped cell biological studies and impeded development of expression-based screens, medium- to high-throughput imaging analyses, and the use of Chlamydomonas as a host for expression of foreign proteins. (g3journal.org)
  • Assembly of flagellar radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3. (rupress.org)
  • We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. (asm.org)
  • Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi, and have visualized its component glycoproteins after mechanical shearing and after depolymerization induced by perchlorate or by the wall-disrupting agent, autolysin. (mendeley.com)
  • Crossing-over in Chlamydomonas reinhardi . (springer.com)
  • A genetic analysis of linkage group I of Chlamydomonas reinhardi . (springer.com)
  • Biochemical mutants of Chlamydomonas reinhardi . (springer.com)
  • Multiple mutation in Chlamydomonas reinhardi . (springer.com)
  • The relation of calcium and magnesium to crossingover in Chlamydomonas reinhardi . (springer.com)
  • Inheritance in the green alga Chlamydomonas reinhardi . (springer.com)
  • Conservation des souches de Chlamydomonas reinhardi a basse temperature. (springer.com)
  • Mutants of the biological clock in Chlamydomonas reinhardi . (springer.com)
  • Study of pigmentation-modifying mutations in Chlamydomonas reinhardi strains of different ploidy. (springer.com)
  • UV-sensitive mutants of Chlamydomonas reinhardi . (springer.com)
  • Genetical and structural analysis of cell-wall formation in Chlamydomonas reinhardi . (springer.com)
  • Linkage maps in Chlamydomonas reinhardi . (springer.com)
  • When these engineered genes were transformed into a Chlamydomonas mutant that lacks small-subunit genes, photosynthesis-competent colonies were recovered, indicating that loop size is not essential for holoenzyme assembly. (rcsb.org)
  • In this study, in order to investigate and compare the toxic effects of the two salts on algal photosynthesis, we used NaCl and Na2CO3 to stress Chlamydomonas reinhardtii. (degruyter.com)
  • Numerous forward genetics studies have been performed in Chlamydomonas, in many cases to elucidate the regulation of photosynthesis. (plantphysiol.org)
  • In this report, we focus on mutants affecting photosynthesis, in keeping with the thrust of this journal, and the emphasis of the newly renewed and National Science Foundation-supported Chlamydomonas genome project ( http://www.chlamy.org/ ). (plantphysiol.org)
  • Reference : Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinha. (ac.be)
  • The nitrate reductase gene from wild-type Chlamydomonas was used to complement a mutation in the corresponding gene of a strain containing nit1-305. (pnas.org)
  • This high frequency of cotransformation will allow any cloned gene to be introduced into Chlamydomonas. (pnas.org)
  • Forty single gene mutations in Chlamydomonas reinhardtii were isolated based on resistance to the compound 5'-methyl anthranilic acid (5-MAA). (genetics.org)
  • The Chlamydomonas reinhardtii nar-2, nar-3, and nar-4 genes, which are within a nitrate-regulated gene cluster containing the nitrate reductase structural gene nit-1, have been related to nitrate transport. (nih.gov)
  • Our Invitrogen GeneArt Chlamydomonas Protein Expression Vector offers transgene expression from the nuclear genome but is optimized for high-level expression, provides selection against gene silencing, and offers dual protein tags for detection and/or purification of your gene of interest. (thermofisher.com)
  • The gene for the hydrolytic enzyme xylanase ( xyn1 ) from Trichoderma reesei was cloned into the vector pChlamy_4 and transformed into Chlamydomonas reinhardtii 137c. (thermofisher.com)
  • Phylogenetic reconstruction of changes in plastid genome content revealed that an accelerated rate of gene loss also characterized the Chlamydomonas/Chlorella lineage, a phenomenon that might be independent of the proliferation of SDRs. (plantcell.org)
  • Tools that have not been previously applied to Chlamydomonas, such as bulked segregant analysis and marker duplexing, are being implemented to increase the speed at which one can go from mutant phenotype to gene. (plantphysiol.org)
  • To analyze the function of ciliary polycystic kidney disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned the gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with the characteristics of PKD2 family members. (rupress.org)
  • At least nine different lhca -related gene products were predicted by comparison of the mass spectrometric data against Chlamydomonas expressed sequence tag and genomic databases, demonstrating the extensive variability of the C. reinhardtii Lhca antenna system. (asm.org)
  • To resolve this conundrum, we identified a mutant of Chlamydomonas deleted in the TGD2 gene and characterized the respective protein, CrTGD2. (unl.edu)
  • A heat inducible expression vector containing amiRNA targeting OEE2 gene was constructed and transformed into Chlamydomonas reinhardtii . (oilgae.com)
  • The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. (asm.org)
  • In addition a protein activity assay was developed in order to confirm that the Chlamydomonas RppH homolog has RNA pyrophosphohydrolase activity. (uio.no)
  • We present evidence suggesting that in Chlamydomonas reinhardtii this coupling may be aided by a hyper-phosphorylated form of the LHCII-like CP29 protein (Lhcbm4). (diva-portal.org)
  • Chlamydomonas reinhardtii contains a factor that can replace adenosine 3':5'-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. (semanticscholar.org)
  • The flagellar motility of Chlamydomonas pf25 mutant lacking an AKAP-binding protein is overtly sensitive to medium conditions. (semanticscholar.org)
  • Through searching of Chlamydomonas databases, 10 genes that potentially encode Lhcb polypeptides that are associated with the major trimeric PSII antenna (Lhcbm) have been described ( 11 ). (asm.org)
  • Genetic transformation into the Chlamydomonas nucleus has been used in many studies, and methods and reagents including promoters, terminators, enhancers, reporter genes, and auxotrophic and drug-resistance markers are available (for review, see Jinkerson and Jonikas 2015 ). (g3journal.org)
  • RNA-seq characterized the transcriptomic response by Chlamydomonas during aggregation, and we identified 131 genes are significantly differentially expressed between predated and unpredated cultures of Chlamydomonas. (k-state.edu)
  • Evolutionary relationships between candidate aggregation genes in Chlamydomonas and their orthologs in multicellular Volvocales suggest a possible role of aggregation genes in multicellular development. (k-state.edu)
  • Our results demonstrate that Chlamydomonas dynamically alters its morphology based on its environment and identify several candidate genes for aggregation and multicellular development. (k-state.edu)
  • In addition, Chlamydomonas chloroplasts contain a number of mRNA specific translation factors that are not found in bacteria. (springer.com)
  • Our working hypothesis is that the siRNA signal transmitted to Chlamydomonas has the same effect on the mRNA as the physical siRNA. (scirp.org)
  • Here we present the optimization of the siRNA signal transduction to Chlamydomonas while we measure the growth rate and then fine tune the conditions measuring the amount of mRNA produced under the influence of the signal specific for the mRNA. (scirp.org)
  • Here, I also show that mRNA abundance in UWO 241 does not respond to different steady-state growth temperatures or a heat-shock temperature shift regime in ways similar to the related mesophile, Chlamydomonas reinhardtii . (uwo.ca)
  • en] Photosynthetic activities were analyzed in Chlamydomonas reinhardtii mitochondrial mutants affected in different complexes (I, III, IV, I + III, and I + IV) of the respiratory chain. (ac.be)
  • Chlamydomonas debaryana strain NIES-2212, whole genome shotgun sequencing projec. (nih.gov)
  • The authors would like to thank Dr. Francesca Zito (CNRS, France) for providing the Chlamydomonas strain and her insights on Chlamydomonas growth and physiology. (springer.com)
  • The strain CC-125 [5] of Chlamydomonas was obtained from the Chlamydomonas Resource Center, University of Minnesota, 140 Gortner Laboratory, St. Paul, MN 55108. (scirp.org)
  • A combined 18S rDNA and rbcL phylogenetic analysis of Chloromonas and Chlamydomonas (Chlorophyceae, Volvocales ) emphasizing snow and other cold-termperature habitats. (wikipedia.org)
  • Characterization of a mutant of Chlamydomonas reinhardtii resistant to fusidic acid. (springer.com)
  • After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. (unl.edu)
  • We wish to recruit a student with some laboratory experience (BS or MS or eq. for instance) to study mitochondrial complex I assembly in the green alga Chlamydomonas (see below for a scientific summary of the project). (bio.net)
  • Chlamydomonas reinhardtii is a single-cell green alga about 10 micrometres in diameter that swims with two flagella. (wikipedia.org)
  • Chlamydomonas reinhardtii is a single-celled green alga that phototaxes toward light by means of a light-sensitive organelle, the eyespot. (genetics.org)
  • CHLAMYDOMONAS reinhardtii is a phototactic, single-celled green alga. (genetics.org)
  • chlamydomonas is a genus of green alga . (biology-online.org)
  • Chlamydomonas reinhardtii has become a sophisticated model system relevant to studies of biological processes and has been referred to as the 'green yeast. (carnegiescience.edu)
  • Some species of eukaryote-such as Chlamydomonas reinhardtii , a single-celled green alga, and Trypanosoma brucei , the protist parasite that causes African sleeping sickness-must grow new flagella when their cells divide, so that each new cell can swim. (elifesciences.org)
  • The green flagellate Chlamydomonas reinhardtii is a model organism for studying the eukaryotic flagella. (uni-koeln.de)
  • The psychrophilic green alga Chlamydomonas sp. (uwo.ca)
  • Like many microorganisms, screening of Chlamydomonas strains for rare mutations is straightforward, since large numbers of cells can be plated on an appropriate selective medium, or nonswimmers, for example, can be selected from large numbers of swimming cells. (plantphysiol.org)
  • Taken together, the assortment of techniques useable for Chlamydomonas indulges both the amateur and experienced geneticist, yielding sometimes overwhelming collections of mutant strains. (plantphysiol.org)
  • In 1990, UTEX, CCAP, and SAG strains of Chlamydomonas were investigated by H. Ettl & U.G. Schlosser and many were found to be incorrectly named. (utex.org)
  • The red eyespot in Chlamydomonas is sensitive to light and hence determines movement. (wikipedia.org)
  • Chlamydomonas reinhardtii has an eyespot similar to that of dinoflagellates(1). (wikipedia.org)
  • Chlamydomonas paralyzed-flagella ( pf ) mutants lacking CP or RS are non-motile under physiological conditions. (nature.com)
  • Flagella of Chlamydomonas paralyzed-flagella ( pf ) mutants beat at high pressure. (nature.com)
  • It also has some community oriented activities which includes a public talk by Susan Dutcher on June 17 that deals with the flagella/cilia and what it has contributed to our understanding of diseases resulting from cilia dysfunction, and a workshop on the use of the new Chlamydomonas indexed, mapped library. (carnegiescience.edu)
  • Fully grown steady-state Chlamydomonas flagella release flagellar vesicles from their tips and failure to resupply membrane should affect flagellar length. (ku.edu)
  • Brefeldin-A destroyed Chlamydomonas Golgi, inhibited the secretory pathway, inhibited flagellar regeneration, and induced full-length flagella to disassemble within 6 hrs, consistent with flagellar disassembly being induced by a failure to resupply membrane. (ku.edu)
  • Colchicine-binding tubulin was isolated from the centre 2 axoneme microtubules from the flagella of the alga Chlamydomonas eugametos. (biologists.org)
  • The 10th International Conference on the Cell and Molecular Biology of Chlamydomonas will be held in Vancouver, Canada, June 11-16, 2002. (bio.net)
  • Chlamydomonas is used as a model organism for research on fundamental questions in cell and molecular biology such as: How do cells move? (wikipedia.org)
  • We next review the cytology and cell biology of the multiple-fission cell cycle of Chlamydomonas. (nih.gov)
  • The International Conference on the Cell and Molecular Biology of Chlamydomonas is held every two years. (genetics-gsa.org)
  • These aspects of Chlamydomonas biology have been previously reviewed ( Rochaix, 1995 ). (plantphysiol.org)
  • However, the map-based cloning tools described here are generally applicable to Chlamydomonas biology. (plantphysiol.org)
  • The Chlamydomonas genome has been sequenced, assembled, and annotated to produce a rich resource for genetics and molecular biology in this well-studied model organism. (g3journal.org)
  • Chlamydomonas acidophila Chlamydomonas caudata Wille Chlamydomonas ehrenbergii Gorozhankin Chlamydomonas elegans G.S.West 1915 Chlamydomonas moewusii Chlamydomonas nivalis Chlamydomonas ovoidae Chlamydomonas reinhardtii Chlamydomonas is widely distributed in freshwater or damp soil. (wikipedia.org)
  • Obligate autotrophy in Chlamydomonas moewusii Gerloff. (springer.com)
  • ISBN 978-1-904890-31-7 Harris, Elizabeth H. ( 2009) "The Genus Chlamydomonas" In The Chlamydomonas Sourcebook (Second Edition), chapter 1, volume 1, pages 1-24. (wikipedia.org)
  • 3)Hegemann P, Berthold P (2009) Sensory photoreceptors and light control of flagellar activity.The Chlamydomonas Sourcebook, ed Witman G-B (Academic, Oxford), 2nd Ed, Vol 3, pp 395-430. (wikipedia.org)
  • This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. (elsevier.com)
  • When this happens some organisms, such as the single-cell alga Chlamydomonas, are able to generate cellular energy from the breakdown of sugars without taking up oxygen. (algaeindustrymagazine.com)
  • To cope with changes in its aquatic environment and the nutrient deficiencies that may result, Chlamydomonas reinhardtii, a mobile single-cell alga, must adapt its metabolism for subsistence, notably in terms of sugar. (algaeindustrymagazine.com)
  • Partial purification of intact chloroplasts from Chlamydomonas reinhardtii . (springer.com)
  • Chlamydomonas reinhardtii is an especially well studied biological model organism, partly due to its ease of culturing and the ability to manipulate its genetics. (wikipedia.org)
  • In this work we examined the possibility of studying, by solid-state NMR, the model organism Chlamydomonas reinhardtii fully and non-specifically 13 C labelled. (springer.com)
  • New work from a team including Carnegie Institution for Science's Wenqiang Yang and Arthur Grossman, in collaboration with Matt Posewitz at the Colorado School of Mines, hones in on the biochemical pathways underlying the special flexibility of Chlamydomonas in responding to oxygen-free and low-oxygen conditions. (algaeindustrymagazine.com)
  • Although many tools are available to facilitate genetic, molecular biological, biochemical, and cell biological studies in Chlamydomonas , expression of unselected transgenes of interest (GOIs) has been challenging. (g3journal.org)
  • Since Chlamydomonas species are normally haploid, the effects of mutations are seen immediately without further crosses. (wikipedia.org)
  • Tryptophan analog resistance mutations in Chlamydomonas reinhardtii. (genetics.org)
  • By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. (pnas.org)
  • In wild-type Chlamydomonas cells, two to four layers of carotenoid lipid granules are observed, each layer subtended by a thylakoid membrane. (genetics.org)
  • Chlamydomonas cells rotate twice per second as they breaststroke forward, scanning the environment perpendicular to the swimming path much as a radar antenna scans the sky. (genetics.org)
  • Chlamydomonas reinhardii cells, growing photoautotrophically under air, excreted to the culture medium much higher amounts of NO 2 − and NH 4 + under blue than under red light. (plantphysiol.org)
  • Nitrite plays an important role in the nitrogen metabolism of most cells, including Chlamydomonas reinhardtii. (mdpi.com)
  • One of these phenomena, the differentiation of vegetative cells into sexually mature cells has been widely studied in Chlamydomonas and may proceed in two steps [ 10 ]. (mdpi.com)
  • The analysis of the detected traces revealed two main vectorial components of the signal by the help of singular value decomposition (SVD), in concert with previous experimental findings and theoretical considerations suggesting different origins of the "fast" and "slow" components of the photoelectric response of Chlamydomonas and Haematococcus cells. (biomedsearch.com)
  • Molecular phylogeny studies indicated that the traditional genus Chlamydomonas defined using morphological data was polyphyletic within Volvocales , and many species were reclassified (e.g., in Oogamochlamys , Lobochlamys ), and many other " Chlamydomonas " lineages are to be reclassificated. (wikipedia.org)
  • Chlamydomonas species are widely distributed worldwide in soil and fresh water. (wikipedia.org)
  • The type species (holotype) of the genus Chlamydomonas is Chlamydomonas reinhardtii P.A.Dangeard . (algaebase.org)
  • Adenosine 3':5'-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization. (semanticscholar.org)
  • Blaby IK et al (2014) The Chlamydomonas genome project: a decade on. (springer.com)
  • High-frequency nuclear transformation of Chlamydomonas reinhardtii. (pnas.org)
  • At the same time, the ease of nuclear transformation in Chlamydomonas, coupled with the plant-like nonhomologous integration of transforming DNA, facilitates the creation of insertional mutant collections. (plantphysiol.org)
  • A plasmid vector containing Chlamydomonas rppH-6xHN was introduced into C. reinhardtii by nuclear transformation. (uio.no)
  • Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. (rupress.org)
  • In an arduous and exacting step-by-step process, the team used a series of specially created mutants to determine the importance of two identical branches of the fermentation pathway that are located in different compartments in the cell, both believed to be essential to dark, low-oxygen fermentation in Chlamydomonas. (algaeindustrymagazine.com)
  • Molecular phylogeny and taxonomic revision of Chlamydomonas (Chlorophyta). (wikipedia.org)
  • Lastly, we review recent genetic approaches and insights into Chlamydomonas cell-cycle regulation that have been enabled by a new generation of genomics-based tools. (nih.gov)
  • CP12 residues involved in the formation and regulation of the glyceraldehyde-3-phosphate dehydrogenase-CP12-phosphoribulokinase complex in Chlamydomonas reinhardtii. (sigmaaldrich.com)
  • Labeling kinetics indicate that Chlamydomonas can import lipid precursors from the ER, a process that is impaired in the tgd2 mutant. (unl.edu)
  • Vererbung des Geschlechts bei Chlamydomonas eugametos und verwandten Arten. (springer.com)
  • I. Emendation of Chlamydomonas Ehrenberg and Chloromonas Gobi, and descripription of Oogamochlamys gen. nov. and Lobochlamys gen. nov. (wikipedia.org)
  • Die Gattung Chlamydomonas Ehrenberg. (springer.com)
  • Relationship between phospholipid breakdown and freezing injury in a cell wall-less mutant of Chlamydomonas reinhardii . (springer.com)