ChitinaseChitin: A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.Trisaccharides: Oligosaccharides containing three monosaccharide units linked by glycosidic bonds.Acetylglucosamine: The N-acetyl derivative of glucosamine.Glucan 1,3-beta-Glucosidase: An exocellulase with specificity for 1,3-beta-D-glucasidic linkages. It catalyzes hydrolysis of beta-D-glucose units from the non-reducing ends of 1,3-beta-D-glucans, releasing GLUCOSE.Schizophyllum: A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.Serratia marcescens: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.Trichoderma: A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Dioscorea: A plant genus best known for edible underground tubers. Yam may also refer to a moist variety of sweet potato, IPOMOEA BATATAS.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Adipokines: Polypeptides produced by the ADIPOCYTES. They include LEPTIN; ADIPONECTIN; RESISTIN; and many cytokines of the immune system, such as TUMOR NECROSIS FACTOR-ALPHA; INTERLEUKIN-6; and COMPLEMENT FACTOR D (also known as ADIPSIN). They have potent autocrine, paracrine, and endocrine functions.Waxes: A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)Vanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Paeonia: A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.Chitin Synthase: An enzyme that converts UDP glucosamine into chitin and UDP. EC 2.4.1.16.Crustacea: A large subphylum of mostly marine ARTHROPODS containing over 42,000 species. They include familiar arthropods such as lobsters (NEPHROPIDAE), crabs (BRACHYURA), shrimp (PENAEIDAE), and barnacles (THORACICA).Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Cryptococcus gattii: A species of the fungus CRYPTOCOCCUS. Its teleomorph is Filobasidiella bacillispora.Indian Ocean Islands: Numerous islands in the Indian Ocean situated east of Madagascar, north to the Arabian Sea and east to Sri Lanka. Included are COMOROS (republic), MADAGASCAR (republic), Maldives (republic), MAURITIUS (parliamentary democracy), Pemba (administered by Tanzania), REUNION (a department of France), and SEYCHELLES (republic).Mycoplasma penetrans: A species of gram-negative, facultatively anaerobic bacteria. This organism shows remarkable pathobiologic properties: it adheres to cell surfaces, deeply penetrates into the cell, and strongly adsorbs human red blood cells and human CD4+ lymphocytes and monocytes. M. penetrans was first isolated from the urogenital tract of patients with AIDS and high frequencies of antibodies to it are seen in HIV-infected patients.Cryptococcosis: Infection with a fungus of the species CRYPTOCOCCUS NEOFORMANS.Cryptococcus: A mitosporic Tremellales fungal genus whose species usually have a capsule and do not form pseudomycellium. Teleomorphs include Filobasidiella and Fidobasidium.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Dipetalonema: A filarial nematode parasite of mammalian blood with the vector being a tick or small fly.Dipetalonema Infections: Infections with nematodes of the genus DIPETALONEMA.Acanthocheilonema: A common genus of tropical filarial worms parasitic in humans and monkeys.Helminth Proteins: Proteins found in any species of helminth.Acanthocheilonemiasis: A tropical infectious disease found mainly in Africa that is caused by the filarial parasite ACANTHOCHEILONEMA. Symptoms include skin rashes, abdominal, chest, muscle, and joint pains, neurologic disorders, skin lumps, and elevated levels of white blood cells. The parasite is transmitted through the bite of small flies.Filarioidea: A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.Cystatin C: An extracellular cystatin subtype that is abundantly expressed in bodily fluids. It may play a role in the inhibition of interstitial CYSTEINE PROTEASES.Salt Gland: A compound tubular gland, located around the eyes and nasal passages in marine animals and birds, the physiology of which figures in water-electrolyte balance. The Pekin duck serves as a common research animal in salt gland studies. A rectal gland or rectal salt gland in the dogfish shark is attached at the junction of the intestine and cloaca and aids the kidneys in removing excess salts from the blood. (Storer, Usinger, Stebbins & Nybakken: General Zoology, 6th ed, p658)Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Peptides, Cyclic: Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Fungicides, Industrial: Chemicals that kill or inhibit the growth of fungi in agricultural applications, on wood, plastics, or other materials, in swimming pools, etc.Plastids: Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Tobacco: A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Plant Diseases: Diseases of plants.Genome, Plastid: The genetic complement of PLASTIDS as represented in their DNA.

Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism. (1/876)

The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased up to 42-fold in multicopy strains as compared with the wild type, whereas gene disruptants exhibited practically no activity. The densities of chitin labeling of Rhizoctonia solani cell walls, after interactions with gene disruptants were not statistically significantly different than the density of chitin labeling after interactions with the wild type. Finally, no major differences in the efficacies of the strains generated as biocontrol agents against R. solani or Sclerotium rolfsii were observed in greenhouse experiments.  (+info)

Granulomatous inflammatory response to recombinant filarial proteins of Brugia species. (2/876)

The lymphatic inflammatory response in Brugia-infected jirds peaks early during primary infections and then decreases in severity as judged by the numbers of lymph thrombi present within these vessels. Antigen-specific hypersensitivity reactions in these animals was measured by a pulmonary granulomatous inflammatory response (PGRN) induced by somatic adult worm antigen (SAWA)-coated beads, and by cellular proliferative responses of renal lymph node cells. The kinetics of these responses temporally correspond to lymphatic lesion formation. The importance of any single antigen to the induction of this inflammatory response has not been elucidated. In this study, the PGRN was used to measure the cellular immune response to four recombinant filarial proteins during the course of a primary B. pahangi infection. These proteins were BpL4, glycoprotein (glutathione peroxidase) gp29, heat shock protein (hsp) 70, and filarial chitinase. All were fusion proteins of maltose-binding protein (MBP). Control beads included those coated with diethanolamine (DEA), SAWA, or MBP. The measurements of PRGN were made at 14, 28, 56, and > 150 days postinfection (PI) in infected jirds, in jirds sensitized with SAWA, and in uninfected jirds. The secretory homolog of glutathione peroxidase gp29 was the only recombinant protein tested that induced a significantly greater PGRN (P < 0.05) than controls. This was seen at 28 days PI. These observations indicate that gp29 may be part of the worm antigen complex that induces an early inflammatory response, a response similar to that observed with SAWA. These studies indicate that this approach is useful in investigating the functional ability of specific proteins in the induction and down-regulation of immune-mediated inflammatory responses elicited by filarial parasites. Absence of a granulomatous response to the other recombinant proteins used may be related to the nature and sensitivity of the assay used or the character of recombinant proteins tested.  (+info)

Stochastic and nonstochastic post-transcriptional silencing of chitinase and beta-1,3-glucanase genes involves increased RNA turnover-possible role for ribosome-independent RNA degradation. (3/876)

Stochastic and nonstochastic post-transcriptional gene silencing (PTGS) in Nicotiana sylvestris plants carrying tobacco class I chitinase (CHN) and beta-1,3-glucanase transgenes differs in incidence, stability, and pattern of expression. Measurements with inhibitors of RNA synthesis (cordycepin, actinomycin D, and alpha-amanitin) showed that both forms of PTGS are associated with increased sequence-specific degradation of transcripts, suggesting that increased RNA turnover may be a general feature of PTGS. The protein synthesis inhibitors cycloheximide and verrucarin A did not inhibit degradation of CHN RNA targeted for PTGS, confirming that PTGS-related RNA degradation does not depend on ongoing protein synthesis. Because verrucarin A, unlike cycloheximide, dissociates mRNA from ribosomes, our results also suggest that ribosome-associated RNA degradation pathways may not be involved in CHN PTGS.  (+info)

Strong induction of members of the chitinase family of proteins in atherosclerosis: chitotriosidase and human cartilage gp-39 expressed in lesion macrophages. (4/876)

Atherosclerosis is initiated by the infiltration of monocytes into the subendothelial space of the vessel wall and subsequent lipid accumulation of the activated macrophages. The molecular mechanisms involved in the anomalous behavior of macrophages in atherogenesis have only partially been disclosed. Chitotriosidase and human cartilage gp-39 (HC gp-39) are members of the chitinase family of proteins and are expressed in lipid-laden macrophages accumulated in various organs during Gaucher disease. In addition, as shown in this study, chitotriosidase and HC gp-39 can be induced with distinct kinetics in cultured macrophages. We investigated the expression of these chitinase-like genes in the human atherosclerotic vessel wall by in situ hybridizations on atherosclerotic specimens derived from femoral artery (4 specimens), aorta (4 specimens), iliac artery (3 specimens), carotid artery (4 specimens), and coronary artery (1 specimen), as well as 5 specimens derived from apparently normal vascular tissue. We show for the first time that chitotriosidase and HC gp-39 expression was strongly upregulated in distinct subsets of macrophages in the atherosclerotic plaque. The expression patterns of chitotriosidase and HC gp-39 were compared and shown to be different from the patterns observed for the extracellular matrix protein osteopontin and the macrophage marker tartrate-resistant acid phosphatase. Our data emphasize the remarkable phenotypic variation among macrophages present in the atherosclerotic lesion. Furthermore, chitotriosidase enzyme activity was shown to be elevated up to 55-fold in extracts of atherosclerotic tissue. Although a function for chitotriosidase and HC gp-39 has not been identified, we hypothesize a role in cell migration and tissue remodeling during atherogenesis.  (+info)

Molecular cloning and ethylene-inducible expression of Chib1 chitinase from soybean (Glycine max (L.) Merr.). (5/876)

A soybean seed-specific PR-8 chitinase, named Chib2, has a markedly extended C-terminal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date. To further characterize the molecular structure and the expression pattern of this chitinase family, we cloned two typical Chib1-similar cDNAs (Chib1-1 and Chib1-2) from soybeans by PCR-cloning techniques. The deduced primary sequence of Chib1-1 chitinase is composed of a signal peptide segment (26 amino acid residues) and a mature 273 amino acid sequence (calculated molecular mass 28,794, calculated pI 3.7). This Chib1-1 enzyme is more than 90% identical to Chib1-2 chitinase but is below 50% identical to Chib2 enzyme. Thus, we confirmed the occurrence of two distinct classes, Chib1 and Chib2 in the plant PR-8 chitinase family. The Chib1 genes, interrupted by one intron, were found to be up-regulated in response to ethylene in stems and leaves, but scarcely expressed in developing soybean seeds. Chib1 chitinases may be responsible for protecting the plant body from various pathogenic attacks.  (+info)

A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes. (6/876)

We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that the N-terminal domain of this precursor has high sequence similarity with the N-terminal domain of class I PR-4 (pathogenesis-related) proteins, whereas the C terminus is most closely related to that of class V chitinases. On the basis of these sequence homologies the gene encoding SN-HLPf can be considered a hybrid between a PR-4 and a class V chitinase gene.  (+info)

Distribution of chitinase in guinea pig tissues and increases in levels of this enzyme after systemic infection with Aspergillus fumigatus. (7/876)

Intravenous infection of guinea pigs with the fungus Aspergillus fumigatus resulted in increased levels of chitinase in serum and tissues of the animals. The molecular properties of the enzyme were demonstrated to be different from those of the fungal chitinase, but also from guinea pig lysozyme and beta-N-acetylhexosaminidase. Bio-Gel P-100 gel filtration showed that in liver, spleen, heart and lung tissue of control animals there were two molecular mass forms present with apparent molecular masses of 35 kDa and 15 kDa. In brain and serum, only the 35 kDa form was detectable. Kidney showed only the 15 kDa form. Upon infection the 35 kDa form appeared in kidney and increased in the other tissues. When a less pathogenic form of the fungus was used the 35 kDa form remained absent in kidney. In contrast to human serum chitinase, the enzyme from guinea pig serum and tissues did bind to concanavalin A-Sepharose. This was the case for both molecular mass forms. The mode of cleavage of the substrate 4-methylumbelliferyl-tri-N-acetylchitotrioside (MU-[GlcNAc]3, where GlcNAc is N-acetylglucosamine) by the two forms of the enzyme was the same: both [GlcNAc]2 and [GlcNAc]3 were released. The chitinase activity levels in the control tissues showed a large variation in this order: spleen > lung, kidney > liver > heart > brain. The fact that spleen showed the highest chitinase level is in agreement with its major role as a lymphoid organ in cases of systemic infections. The relative increases upon infection were the highest for the tissues that showed low control values.  (+info)

Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91. (8/876)

A cluster of three closely linked chitinase genes organized in the order chiA, chiB and chiC, with the same transcriptional direction, and two unlinked genes, chiP and chiQ, involved in chitin degradation in Pseudoalteromnas sp. strain S91 were cloned, sequenced and characterized. The deduced amino acid sequences revealed that ChiA, ChiB and ChiC exhibited similarities to chitinases belonging to family 18 of the glycosyl hydrolases while ChiP and ChiQ belonged to family 20. ChiP and ChiQ showed different enzymic activities against fluorescent chitin analogues, but neither was able to degrade colloidal chitin. ChiA possessed chitinase activity but did not bind chitin; ChiB bound chitin but had no chitinase activity; ChiC possessed strong chitinase activity and also bound chitin. Production of ChiC in S91 appeared to be controlled by chiA expression, since insertion of a transposon into the ORF of chiA resulted in the loss of chitinase activity as well as loss of ChiC proteins in a chitinase-negative mutant. In Escherichia coli, ChiC appeared to be expressed from its own promoter.  (+info)

*Chitinase

... at the US National Library of Medicine Medical Subject Headings (MeSH) The X-ray structure of a chitinase from the ... Stress, or environmental signals like ethylene gas, may stimulate increased production of chitinase. Some parts of chitinase ... as well as chitinase-like proteins (such as YKL-40) that have high sequence similarity but lack chitinase activity. Like ... Chitinase activity can also be detected in human blood and possibly cartilage. As in plant chitinases this may be related to ...

*Chitinase A N-terminal domain

In molecular biology, the chitinase A N-terminal domain is found at the N-terminus of a number of bacterial chitinases and ... "Crystal structure of a bacterial chitinase at 2.3 A resolution". Structure. 2 (12): 1169-80. doi:10.1016/s0969-2126(94)00119-7 ...

*Chitinase domain-containing protein 1

... (CHID1) is a highly conserved protein of unknown function located on the short (p) arm of ... Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X (Dec 2010). "Structure of human stabilin-1 interacting chitinase-like ... It has several aliases, the most common of which is Stabilin-1 interacting chitinase-like protein (SI-CLP). As indicated by the ... This gene also has several aliases, the most common of which is Stabilin-1 interacting chitinase-like protein, or SI-CLP, which ...

*Glycoside hydrolase family 19

... CAZY GH_19 comprises enzymes with only one known activity; chitinase (EC 3.2.1.14). Chitinases ... Jolles P, Flach J, Pilet PE (1992). "What's new in chitinase research?". Experientia. 48 (8): 701-716. doi:10.1007/BF02124285. ...

*Laceyella putida

... produces chitinase. Parte, A.C. "Laceyella". www.bacterio.net. Retrieved 28 November 2016. "Laceyella putida ... "Highly thermostable and surfactant-activated chitinase from a subseafloor bacterium, Laceyella putida". Applied Microbiology ...

*Streptomyces olivaceoviridis

... produces chitinase and xylanase. BLAAK, Harald; SCHNELLMANN, Jurgen; WALTER, Stefan; HENRISSAT, ... Hassan, F; Meens, J; Jacobsen, HJ; Kiesecker, H (25 September 2009). "A family 19 chitinase (Chit30) from Streptomyces ...

*Streptomyces thermoviolaceus

... produces chitinase and peroxidase. Wang, W; Mai-Gisondi, G; Stogios, PJ; Kaur, A; Xu, X; Cui, H; ... Tsujibo, H (1 December 1999). "Synthesis of chitinase in Streptomyces thermoviolaceus is regulated by a two-component sensor- ... "Purification and properties of a thermostable chitinase from Streptomyces thermoviolaceus OPC-520". Applied and Environmental ...

*Sulfolobus tokodaii

Staufenberger, Tim; Imhoff, Johannes F.; Labes, Antje (2012). "First crenarchaeal chitinase found in Sulfolobus tokodaii". ...

*Carbohydrate-binding module

Butler AR, O'Donnell RW, Martin VJ, Gooday GW, Stark MJ (July 1991). "Kluyveromyces lactis toxin has an essential chitinase ... Kuranda, M. J.; Robbins, P. W. (1991). "Chitinase is required for cell separation during growth of Saccharomyces cerevisiae". ... "Identification of the Substrate Interaction Region of the Chitin-Binding Domain of Streptomyces griseus Chitinase C". Journal ... encodes both a lectin and a chitinase". J. Biol. Chem. 267 (16): 11085-91. PMID 1375935. ...

*Streptomyces cinereoruber

Okazaki, Katsuichiro; Tagawa, Kiyoshi (January 1991). "Purification and properties of chitinase from Streptomyces cinereoruber ...

*Lysozyme

Skujiņś J, Puķite A, McLaren AD (December 1973). "Adsorption and reactions of chitinase and lysozyme on chitin". Molecular and ...

*Streptomyces violaceusniger

Nagpure, Anand; Gupta, Rajinder K. (May 2013). "Purification and characterization of an extracellular chitinase from ...

*Chaetomium cupreum

Bruce, A.; Srinivasan, U.; Staines, H. J.; Highley, T. L. (1995-01-01). "Chitinase and laminarinase production in liquid ... and chitinase. β 1-3 exoglucanase, endoglucanase IV and β glucosidases are major lytic enzymes targeting the fungal cell wall ... "Chitinase Gene Expression during Mycoparasitic Interaction ofTrichoderma harzianumwith Its Host". Fungal Genetics and Biology. ...

*Streptomyces kurssanovii

Ilyina, AV; Varlamov, VP; Tikhonov, VE; Yamskov, IA; Davankov, VA (April 1994). "One-step isolation of a chitinase by affinity ... Streptomyces kurssanovii produces chitinase, N-(Phenylacetyl)-2-butenediamide and fumaramidmycin. Il'ina, AV; Tatarinova, NIu; ...

*CHI3L1

2003). "Enhanced expression of the human chitinase 3-like 2 gene (YKL-39) but not chitinase 3-like 1 gene (YKL-40) in ... Chitinase-3-like protein 1 (CHI3L1), also known as YKL-40, is a secreted glycoprotein that is approximately 40kDa in size that ... Ma B, Herzog EL, Moore M, Lee CM, Na SH, Lee CG, Elias JA (2016-05-20). "RIG-like Helicase Regulation of Chitinase 3-like 1 ... "Entrez Gene: CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39)". Kazakova MH, Sarafian VS (2009-03-01). "YKL-40--a novel ...

*Thermomyces lanuginosus

Four putative chitinase encoding genes have been identified in T. lanuginosus. Chitinases are glycosyl hydrolases that break ... and chitinase. These enzymes have chemical, environmental, and industrial applications due to their hydrolytic properties. They ... and also have applications in medicine as chitinase has been found to have antifungal properties. T. lanuginosus also produced ...

*Bat

Insectivorous bats may have certain digestive enzymes to better process insects, such as chitinase to break down chitin, which ... "Insectivorous Bats Digest Chitin in the Stomach Using Acidic Mammalian Chitinase". PLoS One. 8 (9): e72770. Bibcode:2013PLoSO ...

*Geomyces pannorum

It produces a range of extracellular hydrolases including lipase, chitinase, and urease. It has been reported as a saprotroph ...

*Streptomyces lydicus

Mahadevan, Brinda; Crawford, Don L. (May 1997). "Properties of the chitinase of the antifungal biocontrol agent Streptomyces ...

*Candex

Lim H, Choi HT (Oct 2010). "Growth inhibition of the yeast transformant by the expression of a chitinase from Coprinellus ... To quote one scientific article: "The purified cellulase was devoid of chitinase, pectinase and mannanase activities". Moreover ... This again suggests cellulases do not in general contain significant chitinase activity. Similarly, although many internet ... when a chitinase gene was bioengineered into the same yeast strain it resulted in complete growth inhibition. ...

*Glycoside hydrolase family 18

Another chitinase II member is the novel gene Chitinase domain-containing protein 1. The family also includes various ... Some members of this family, CAZY GH_18, belong to the chitinase class II group which includes chitinase, chitodextrinase and ...

*Pathophysiology of multiple sclerosis

2015). "Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis". Mult Scler. 21 (10): 1251-61 ...

*Acanthocheilonema viteae

Essential Role of Chitinase in the Development of the Filarial Nematode Acanthocheilonema viteae. Infection and Immunity. 2008 ...

*Proteins in wine

"Roles of Grape Thaumatin-like Protein and Chitinase in White Wine Haze Formation". Journal of Agricultural and Food Chemistry. ...
Plant chitinases are a group of enzymes that presumably hydrolyze chitin, a biopolymer of GlcNAc in a β-1,4 linkage. Only a few of them have been proven to possess chitinase activities. Most of the reported chitinases have been identified based on their sequence similarity to known chitinases. Plant chitinases are grouped into six different classes based on sequence similarity to tobacco chitinases (Meins et al., 1994). The two common classes of chitinases are class I and class II, which differ in the presence (class I) or absence (class II) of a conserved N-terminal cysteine-rich lectin domain. All classes of chitinases possess some conserved amino acid residues in the catalytic domains (Levorson and Chlan, 1997).. Because the chitinase substrate chitin is the main component of many fungal walls and expression of many chitinase genes is induced by pathogens, chitinases have long been proposed to play roles in defense. It has been shown that overexpression of some chitinases alone or together ...
Candida albicans chitinase isolated using the Dyno-Mill disruption technique was characterized using an improved radiometric assay procedure. The enzyme had apparent temperature and pH optima of 45°C and 6·5, respectively. The preparation yielded an apparent K m of 3·9 mg chitin ml-1 [17·6 mM-N-acetylglucosamine (GlcNAc) equivalents] and V of 2·3 nmol GlcNAc formed min-1 (mg protein)-1. The potential of the streptomycete antibiotic allosamidin as an antifungal agent is discussed in view of its dose-dependent inhibition of C. albicans chitinase activity (IC50 = 0·3 μM). Allosamidin was a potent competitive inhibitor of enzyme activity (K i = 0·23 μM).
Summary: A fraction which inhibited chitin synthesis was partially purified from Neurospora crassa by ammonium sulphate precipitation and gel filtration. This preparation possessed chitinase activity and hydrolysed either nascent or preformed chitin. Utilization of UDP-N-acetylglucos-arnine by chitin synthase was not modified in the presence of the chitinase preparation, although the chitin being synthesized was degraded mainly to N-N'-diacetylchitobiose, other larger oligosaccharides and small amounts of N-acetylglucosamine. The enzyme exhibited endo- and exo-chitinase properties and was localized mainly in the cytosol fraction. Its pH optimum was 6.7 and its apparent molecular weight 20600 Dal.
Chitinase, which catalyzes the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. A basic chitinase genomic sequence was isolated from a rice (Oryza sativa L.) genomic library using a bean chitinase gene fragment as a probe. The complete nucleotide sequence of the rice chitinase RCH10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acids. This polypeptide consists of a 21 amino acid signal peptide, a hevein domain, and a chitinase catalytic domain. The RCH10 gene has 63% identity at the nucleotide level and 75% identity at the amino acid level with chitinase genes from dicotyledonous plants such as bean, potato, and tobacco. A gene fusion of trpE and the coding region of RCH10 expressed in Escherichia coli gave a product that reacted with antiserum to bean chitinase, confirming the identity of RCH10 as a rice chitinase gene. Primer ...
Genome-wide analysis of chitinase genes in the Hypocrea jecorina (anamorph: Trichoderma reesei) genome database revealed the presence of 18 ORFs encoding putative chitinases, all of them belonging to glycoside hydrolase family 18. Eleven of these encode yet undescribed chitinases. A systematic nomenclature for the H. jecorina chitinases is proposed, which designates the chitinases corresponding to their glycoside hydrolase family and numbers the isoenzymes according to their pI from Chi18-1 to Chi18-18. Phylogenetic analysis of H. jecorina chitinases, and those from other filamentous fungi, including hypothetical proteins of annotated fungal genome databases, showed that the fungal chitinases can be divided into three groups: groups A and B (corresponding to class V and III chitinases, respectively) also contained the so Trichoderma chitinases identified to date, whereas a novel group C comprises high molecular weight chitinases that have a domain structure similar to Kluyveromyces lactis killer toxins.
Objective. To study the relationship between the human secreted protein stabilin-1-interacting chitinase-like protein (SI-CLP) and rheumatoid arthritis (RA).. Methods. The expression of SI-CLP in peripheral blood mononuclear cells (PBMCs) and synovial fluid from patients with RA and the effects of cytokines on SI-CLP expression were examined by Western blotting. Fluorescence-activated cell sorting analysis was performed to investigate the binding between SI-CLP and cells. Bone marrow-derived macrophages were isolated from wild-type and SI-CLP-/- mice, and real-time quantitative polymerase chain reaction was performed to detect the levels of messenger RNA for cytokines or SI-CLP in SI-CLP- or cytokine-treated macrophages. Histologic studies were conducted to evaluate inflamma-tion and the expression of interleukin-12 (IL-12), IL-13, and SI-CLP in lesions. Enzyme-linked immunosorbent assays were used to detect the cytokine levels in bone marrow-derived macrophages. Rats or mice with ...
Complete information for CHIA gene (Protein Coding), Chitinase, Acidic, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Chitinase is an enzyme having the ability to degrade chitin to a low molecular weight chitooligomers which have a wide range of applications. In the present study, chitinase was produced from the entomopathogenic fungi such as Pochonia chlamydosporia, Beauveria bassiana, Metarhizium anisopliae and Trichoderma harzianum using chitin as a carbon source. The enzyme was partially purified by ammonium sulphate precipitation and the activity was detected using colorimetric method. The enzyme showed the maximum activity in Trichoderma harzianum among the four fungal species at 40°C and pH 6.5. This enzyme could be used directly to control of pathogenic fungi and various pests in the egg stage itself. ...
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Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product cyclopentapeptide, competitively inhibits family 18 chitinases in the nanomolar to micromolar range and shows extensive substrate mimicry
Chitinases cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium (1ctn) and a plant (2hvm). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed. [1] ...
Chitinase domain-containing protein 1 (CHID1) is a highly conserved protein of unknown function located on the short (p) arm of chromosome 11 near the telomere. The protein has 27 introns, which allows for many isoforms of this gene. It has several aliases, the most common of which is Stabilin-1 interacting chitinase-like protein (SI-CLP). As indicated by the alias, CHID1 is known to interact with the protein STAB1. CHID1 is expressed ubiquitously at levels nearly 6 times the average gene, and is conserved very far back to organisms such as Caenorhabditis elegans and possibly some prokaryotes. This protein is known to have carbohydrate binding sites, which could be involved in carbohydrate catabolysis. CHID1 is located on chromosome 11 at the location p15.5. It is just downstream of TSPAN4 and upstream of AP2A2. CHID1 is ubiquitously expressed at a high levels. Through microarray analysis, it has been shown that CHID1 is generally expressed at 5.7 times the average gene. CHID1 has many known ...
Nookaew, I; Thorell, K; Worah, K; Wang, S; Hibberd, ML; Sjövall, H; Pettersson, S; Nielsen, J; Lundin, SB; (2013) Transcriptome signatures in Helicobacter pylori-infected mucosa identifies acidic mammalian chitinase loss as a corpus atrophy marker. BMC Med Genomics, 6. p. 41. ISSN 1755-8794 DOI: 10.1186/1755-8794-6-41 ...
Expression of AamAch-L and AamAch-S mRNA in multiple tick organs observed in this study signals the possibility that native AamAch-L and AamAch-S proteins could be important to tick physiology in general. Our quantitative RT-PCR analysis data are suggestive of the possibility that AamAch-L proteins could be associated with tick feeding physiology at the salivary gland level, and not at the mid-gut level. In tick research, genes that are up-regulated in response to feeding are thought to be associated with blood meal feeding (Mulenga et al., 2007; Aljamali et al., 2009; Mulenga and Khumthong, 2010; Konnai et al., 2011) and those that are down-regulated are believed to play other roles in tick biology, and are not associated with blood meal feeding (Umemiya et al., 2008; Aljamali et al., 2009). Given that AamAch-L mRNA abundance in the SG did not apparently change with feeding could signal the importance of this protein in unfed and during the parasitic stages of A. americanum. We were unable to ...
Enzymes have a very wide range of functions in living organisms. Both signal transduction and regulation of cellular activity rely on enzymes, especially kinases and phosphatases. Enzymes also involve in movement by catalyzing the hydrolysis of ATP on myosin to make muscle contractions and can act as part of the cytoskeleton involved in transporting intracellular substances. ATP enzyme in the cell membrane as the ion pump involves in active transport.. In this articles, there introduces several enzymes biological functions from Creative Enzymes which may greatly help your study researches.. Cellulase. Since the study of cellulase has entered the molecular level, people have gradually explored the structure and function, gene regulation and genetic modification of cellulase.. Chitinase. Chitinase plays a very important physiological role in various organisms. In recent years, a variety of chitinases and chitinase-like proteins have been found in mammals, which plays a very important role in the ...
Fig. 3. Venn diagrams of DEGs in Vitis flexuosa responsive to spore suspension (spore) and culture filtrates (CF) of Elsinoe ampelina, and R. vitis. A, up-regulated genes; B, down-regulated genes. Among 15 up-regulated genes, LRR, LOX, TLP, and GST were particularly up-regulated by spore inoculation than CF treatment of E. ampelina. On the contrary, CHS and TIP were highly up-regulated by CF treatment than spore inocu-lation of E. ampelina. It was reported that chitinase, stilbene synthase, protein/sugar kinase and transcriptional factor genes were found uniquely expressed in anthracnose tolerant upon E. ampelina infection in Florida hybrid bunch grape cultivars(Vasanthiaiah et al., 2010). Chitinase and stilbene synthase genes were reported to be involved in regulation of fungal growth and development in grapevines (Hammerschmidt, 1999; Jayasankar et al., 2000). In this study, chitinase-like protein was induced slightly by spore inoculation, while down-regulated 48 h after by CF treatment of E. ...
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1FFQ: De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin.
Has anyone used chitinases to soften chitin in crustaceans as am having problems with wax sectioning? Have decalcified samples but they are still too hard. Andrew Prior Biology Dept Leicester University UK [email protected] ...
Introduction: Bronchopulmonary dysplasia (BPD) caused by prematurity is associated with more remodeling and fibrosis than asthma, yet symptoms and treatment of these two disorders are similar. The chitinase-like protein YKL-40, is a novel biomarker of asthma although the mechanisms involved are unknown. YKL-40 levels correlate with airway remodeling (Chupp et al. NEJM 2007) and YKL-40 increases smooth muscle proliferation (Bara et al. AJRCCM 2012). We aimed to compare serum YKL-40 in children with asthma and BPD.. Methods: Age- and sex-matched children with diagnosed asthma (n=27) or BPD (n=28) were included in the study at 10 yrs of age. Serum YKL-40 levels were measured by ELISA. ...
3B9E: Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism
Complete information for CHIC2 gene (Protein Coding), Cysteine Rich Hydrophobic Domain 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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Rat anti Human SI-CLP antibody, clone 1C11 detects human Stabilin-interacting chitinase like protein (SI-CLP), a novel member of the Glyco
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Consists primarily of b-1,3 glucanase, chitinase and cellulase. A mycolytic enzyme preparation able to degrade the cell walls of various groups of Eumycetes. The isolated protoplasts have shown a high capacity to regenerate into ...
Gene target information for CHI3L2 - chitinase 3 like 2 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
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Extensive cross-reactivity between the different individual species of the genus could be expected, but has not been formally evaluated. (8). A class I chitinase has been reported to be the relevant protein linked to cross-reactions in latex-fruit allergy syndrome, which includes passion fruit, cherimoya, kiwi, papaya, mango, tomato and wheat. (5, 9). A class I chitinase from avocado or latex extract was used as an inhibitor in a study the aim of which was to evaluate the role of chitinases and complex glycans as cross-reactive determinants linked to latex-food allergy. Putative class I chitinases of 30 to 45 kDa were recognised by both specific polyclonal antibodies to chitinases and sera from patients with latex-fruit allergy in chestnut, cherimoya, passion fruit, kiwi, papaya, mango, tomato, and wheat flour extracts. Prs a 1, the major allergen and class I chitinase from avocado, along with the latex extract, strongly or fully inhibited IgE binding by these components when tested in ...
Ym1 and Fizz1 are secreted proteins that have been identified in a variety of Th2-mediated inflammatory settings. We originally found Ym1 and Fizz1 as highly expressed macrophage genes in a Brugia malayi infection model. Here, we show that their expression is a generalized feature of nematode infection and that they are induced at the site of infection with both the tissue nematode Litomosoides sigmodontis and the gastrointestinal nematode Nippostrongylus brasiliensis. At the sites of infection with N. brasiliensis, we also observed induction of other chitinase and Fizz family members (ChaFFs): acidic mammalian chitinase (AMCase) and Fizz2. The high expression of both Ym1 and AMCase in the lungs of infected mice suggests that abundant chitinase production is an important feature of Th2 immune responses in the lung. In addition to expression of ChaFFs in the tissues, Ym1 and Fizz1 expression was observed in the lymph nodes. Expression both in vitro and in vivo was restricted to antigen-presenting ...
TY - JOUR. T1 - A novel chitinase isozyme in tobacco BY-2 cells induced by the autoclaved Alternaria alternata culture medium. AU - Saito, Mikako. AU - Shinya, Tomonori. AU - Hanai, Kazunari. AU - Katagi, Tetsuya. AU - Matsuoka, Hideaki. PY - 2004/10. Y1 - 2004/10. N2 - BY-2 cells form a linear linkage of several cells. Such a unique configuration is thought to be suitable for the cell-to-cell communication analysis in vivo that is the basis of the elucidation of the systemic acquired resistance in plants. Since chitinase is one of the typical stress response proteins in plants, BY-2 cells were treated with various biotic and abiotic stresses to investigate if chitinase could be induced in BY-2 cells. Among 33 stresses, the autoclaved Alternaria alternata culture medium could induce at least three chitinase isozymes. The amount of each isozyme was very small but the most abundant one, TBC-1, could be successfully isolated at the yield of 2μg from 2.4kg wet matter of BY-2 cells. The N-terminal ...
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BACKGROUND YKL-40, a mammalian member of chitinase-like proteins, has been shown to play a role in pathological conditions leading to tissue remodeling and fibrosis. Recently, YKL-40 was found to be increased in severe asthma, suggesting that YKL-40 contributes to airway remodeling; however, no data are available about YKL-40 expression in idiopathic pulmonary fibrosis (IPF). The present study was conducted to investigate YKL-40 expression in the serum and lung of IPF patients, and to determine its clinical significance. METHODS Using an enzyme-linked immunosorbent assay, we measured YKL-40 levels in the serum of 63 IPF patients and in bronchoalveolar lavage fluid (BALF) of 18 IPF patients. YKL-40 levels were also assessed in the serum and BALF of healthy subjects. We further investigated the relationship between serum YKL-40 levels and clinical parameters. Additionally, immunohistochemical staining for YKL-40 was performed in lung specimens of IPF patients and control subjects. RESULTS Serum and
Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA contains an open reading frame (ORF) of 1449 bp that encodes 483 amino acid residues and 126- and 296-bp non-coding regions at the 5- and 3-ends, respectively. The BdCht2 genome has four exons and three introns. The predicted molecular mass of the deduced BdCht2 is approximately 54.3 kDa, with an isoelectric point of 5.97. The 977 bp 5 flanking region was identified and the transcription factor binding sites were predicted. Bioinformatic analyses showed that the deduced amino acid sequence of BdCht2 had 34%-66% identity to that of chitinases identified in other insect species. Quantitative real-time PCR (qPCR) analyses indicated that BdCht2 was mainly expressed during the larval-pupal and pupal-adult transitions. The tissue-specific
Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA contains an open reading frame (ORF) of 1449 bp that encodes 483 amino acid residues and 126- and 296-bp non-coding regions at the 5- and 3-ends, respectively. The BdCht2 genome has four exons and three introns. The predicted molecular mass of the deduced BdCht2 is approximately 54.3 kDa, with an isoelectric point of 5.97. The 977 bp 5 flanking region was identified and the transcription factor binding sites were predicted. Bioinformatic analyses showed that the deduced amino acid sequence of BdCht2 had 34%-66% identity to that of chitinases identified in other insect species. Quantitative real-time PCR (qPCR) analyses indicated that BdCht2 was mainly expressed during the larval-pupal and pupal-adult transitions. The tissue-specific
This book contains comprehensive contributions on chitin biology and chitin metabolism related enzymes, aiming to provide with recent research progress about biology of chitin-containing organisms as well as cross-talks between chitin-containing and non-chitin-containing organisms.
The 1011 ton global annual turnover of chitin has generated extensive interest in the regulation of chitin processing enzyme production in bacteria. Some bacteria regulate chitinase production by N-Acyl-L-homoserine lactone (AHL) mediated quorum sensing. In this study, a description of bacterial community succession during chitin particle colonisation and depolymerisation in activated sludge is presented. It was discovered that Betaproteobacteria and Bacteroidetes lineages dominate chitin colonisation in sludge and that AHLs bind to chitin at concentrations that upregulate AHL dependent transcription in bacterial cells associated with the chitin surface. There was no requirement for high cell density (a quorum) at the chitin surface. Further, N-Acetyl glucosamine (GlcNAc), the monomer of the chitin polymer, is shown to inhibit AHL dependent gene transcription representing a previously unrecognised mechanism by which the chitinase reaction product negatively regulates chitinase production. ...
Helminth infections and allergic diseases are associated with IgE hyperresponsiveness but the genetics of this phenotype remain to be defined. Susceptibility to Ascaris lumbricoides infection and antibody levels to this helminth are associated with polymorphisms in locus 13q33-34. We aimed to explore this and other genomic regions to identify genetic variants associated with the IgE responsiveness in humans. Forty-eight subjects from Cartagena, Colombia, with extreme values of specific IgE to Ascaris and ABA-1, a resistance marker of this nematode, were selected for targeted resequencing. Burden analyses were done comparing extreme groups for IgE values. One-hundred one SNPs were genotyped in 1258 individuals of two well-characterized populations from Colombia and Sweden. Two low-frequency coding variants in the gene encoding the Acidic Mammalian Chitinase (CHIA rs79500525, rs139812869, tagged by rs10494133) were found enriched in high IgE responders to ABA-1 and confirmed by genetic association ...
A polyclonal antiserum was prepared against a purified microsomal chitinase isolated from the fungus Choanephora cucurbitarum. Indirect immunofluorescence was used to localize chitinase at various developmental stages of five zygomycetous fungi and during abiotrophic mycoparasite interaction with a susceptible and resistant host. This was compared to localization of oligomers of N-acetylglucosamine with the lectin wheat germ agglutinin (WGA). Dotimmunoblot and Western blot techniques revealed that the anti-serum reacted strongly with the antigen from which it was derived. Cross reactivity of the antiserum was found with WGA and another chitin binding lectin, Phyto/acca americana agglutinin (PAA). Immuno-fluorescence results showed the direct involvement of chitinase in spore swelling, germination, sporangium development and response during mechanical injury. There appeared to be no involvement of chitinase during apical hyphal growth or new branch initiation in any of the fungi tested despite ...
1. Amano, Y., and Ozeki, S. 1981. Winter wheat breeding for resistance to snow mold and cold hardiness. I. Development of testing methods and application for the classification of resistant varieties. Bull. Hokkaido Pref. Agric. Exp. Stn. 46:12-21.. 2. Bazzingher, G. 1976. Der schwarze Schneeschimmel der Koniferen [Herpotrichia juniperi (Duby) Petrak und Herpotrichia coulteri (Peck) Bose]. Eu. J. For. Pathol. 6:109-122.. 3. Bruehl, G. W. 1982. Developing wheat resistant to snow mold in Washington state. Plant Dis. 66:1091-1095.. 4. Burpee, L. L., Kaye, L. M., Goulty, L. G., and Lawton, M. B. 1987. Suppression of gray snow mold on creeping bentgrass by an isolate of Typhula phacorrhiza. Plant Dis. 71:97-100.. 5. Cheng, M., Gay, P. A., and McBeath, J. H. 2001. Determination of chitinolytic activity in under differing environmental conditions. P. 57-62. IN: Proceeings of Biocontrol in a New Millenium: Building for the Future on Past Experience. D. M. Huber, ed.. 6. Conn, J., and Cameron, J. 1988. ...
5] Diaz-Perales, A., et al.: Class I chitinases with hevein-like domain, but not class II enzymes, are relevant chestnut and avocado allergens., J. Allergy Clin. Immunol., 102, 127-133 (1998); Chen, Z., et al.: Identification of hevein (Hev b 6.02) in Hevea latex as a major cross-reacting allergen with avocado fruit in patients with latex allergy., J. Allergy Clin. Immunol., 102, 476-481 (1998); Sowka, S., et al.: Identification and cloning of Prs a 1, a 32-kD endochitinase and major allergen of avocado, and its expression in the yeast Pichia pastoris., J. Biol. Chem., 273, 28091-28097 (1998); Mikkola, J.H., et al.: Hevein-like protein domains as a possible cause for allergen cross-reactivity between latex and banana., J. Allergy Clin. Immunol., 102, 1005-1012 (1998); Blanco, C., et al.: Class I chitinases as potentioal panallergens involved in the latex-fruit syndrome., J. Allergy Clin. Immunol., 103, 507-513 (1999); Posch, A., et al.: Class I endochitinase containing a hevein domain is the ...
Insect chitinase 5 (Cht5), a well-characterized enzyme found in the molting fluid and/or integument, is classified as a group I chitinase and is usually encoded by a single gene. In this study, a Cht5 gene cluster consisting ...
Objective To research the interrelation of cholera toxin gene (CT gene) in manifestation of chitinase gene under different pH conditions among pathogenic and Non-pathogenic strains of in time depended chitinase activity, purification of expressed protein and SDS-PAGE analysis. gradients, tolerance to stress and safety from predators[7]. Emergent properties of chemotaxis, cell multiplication, induction of competence, bio?lm formation, commensal and symbiotic relationship with higher organisms, cycling of nutrients, and pathogenicity for humans and aquatic animals[8]. As factors mediating virulence of for humans and aquatic animals derive from mechanisms of adaptation to its environment, at different levels of hierarchical level, relationships with chitin represent a useful model for examination of the part of main habitat selection in the development of traits that have been identi?ed as virulence reasons in human disease[9]. In the current study primarily we targeted different climatic factors ...
小菜蛾 (Plutella xylostella, diamondback),繁殖能力強,生長世代短,可適應各種惡劣的環境,為世界性的十字花科作物重要害蟲,然而其幾丁質相關構造及酵素目前仍未被詳細研究。幾丁質為構成昆蟲表皮及圍食膜 (peritrophic matrix: PM) 之重要物質;幾丁質通常會與幾丁質結合蛋白 (chitin binding proteins: CBPs) 結合,CBPs對於幾丁質重要構造的形成、結構維持以及功能的調控,扮演重要的角色。本研究解析小菜蛾體內幾丁質重要構造之蛋白與幾丁質相關酵素,特別針對圍食膜蛋白 (PM proteins: PMP)、CBPs以及幾丁質? (Chitinase: Chts) 等幾丁質相關蛋白,根據CBPs之基因表現情形、親緣關係分析及幾丁質?選殖與表現定性,期望獲得有助於研發新式鱗翅目害蟲防治之重要資訊。利用2-D電泳與質譜分析,可鑑定到四個 PM ...
Chitin-binding protein which slows larval growth when consumed by the lepidopteran species S.ricini and M.brassica, but not when consumed by the mulberry specialist B.mori. Lacks chitinase activity.
One way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. This can be done using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and IRES elements. In the present study conventional crossing method was used. Parental transgenic lines transformed via Agrobacterium tumefasciens-mediated gene transformation with pGreenII binary vector harbouring a bar gene as selectable marker in combination with the family 19 chitinase gene from Streptomyces olivaceoviridis for one line and 1,3-β-glucanase from barley (Hordeum vulgare) for the other line were used for crossing. Both chitinase and glucanase genes were cloned into pGreenII vector under the control of the constitutive double 35S-promoter from cauliflower mosaic virus. Progenies expressing the two genes were characterised at the molecular level using PCR, RT-PCR and ...
Chitin, a polymer of N-acetylglucosamine, is an important structural component in the cell walls of fungal pathogens. Plant chitinases are capable of degrading this component to directly inhib...
The predominant characteristic feature of C. pinensis is the ability to degrade chitin, a β-1,4-glycosidic linked homopolymer of N-acetyl-D-glucosamine and one of the most abundant polysaccharides in nature. It is a component of fungal cell walls and of arthropod exoskeletons. Chitin is degraded by chitinases (EC 3.2.1.14); endochitinases randomly cleave within the chitin molecule and exochitinases hydrolyze diacetylchitobiose from the end of a chitin chain. Diacetylchitobiose is further degraded to N-acetylglucosamine by the action of N-acetylglucosaminidases (EC 3.2.1.52).. These glycosidic bond hydrolyzing enzymes were grouped in glycoside hydrolase (GH) families based on amino acid sequence similarities (Web Site) [22]. For the C. pinensis genome 169 glycoside hydrolases belonging to 49 different GH families are predicted; 18 of the predicted glycoside hydrolases belong to GH family 43 which contains xylosidases, xylanases, arabinanases, arabinofuranosidases and galactosidases. Because of ...
11118DNAArtificial sequenceSingle strand DNA nucleotide 1ctcagaagac cagagggc 18217DNAArtificial sequenceSingle strand DNA nucleotide 2caaagcggcc atcgtgc 17321DNAArtificial sequenceSingle strand DNA oligonucleotide 3cagaattcgc ccgcccctgc a 21422DNAArtificial sequenceSingle strand DNA oligonucleotide 4ctcagatctt ggcgatgcca ca 22521DNAArtificial sequenceNucleic acid sequence encoding the vacuolar targeting signal peptide derived fromTobacco chitinase A 5gatcttttag tcgatactat g 21666DNAArtificial sequenceNucleic acid sequence encoding the ER signal peptide derived from the Arabidopsis basic endochitinase gene 6atgaagacta atctttttct ctttctcatc ttttcacttc tcctatcatt atcctcggcc 60gaattc 66733PRTArtificial sequenceAcetylcholinesterase leader sequence into the ER 7Met Arg Pro Pro Gln Cys Leu Leu His Thr Pro Ser Leu Ala Ser Pro1 5 10 15Leu Leu Leu Leu Leu Leu Trp Leu Leu Gly Gly Gly Val Gly Ala Glu 20 25 30Gly86PRTArtificial sequenceER retention signal 8Ser Glu Lys Asp Glu Leu1 59606PRTArtificial ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Systemic inflammation is proposed to be one potential major driver of HFpEF, causing coronary microvascular endothelial inflammation and the development of diastolic dysfunction.8,22-25 Indeed, numerous single biomarkers of inflammation have previously (eg, GDF-15, galectin-3, ST2, IL-6, and IL-813,16,17,26,27) and now, to our knowledge, for the first time (eg, chitinase-3-like protein 1 and C-C motif chemokine 20) been found to be associated with HFpEF severity and outcome. GDF-15 was the strongest predictor of outcome among 87 different biomarkers in this study, consistent with previous analyses.26,28 In addition, we present novel prognostic information on the pseudochitinase chitinase-3-like protein 1 (YKL-40), present in inflammatory cells, such as in granules of neutrophils, which has been associated with the increased risk of atrial fibrillation,29 a common comorbidity in HFpEF. Chitinase-3-like protein 1 is a marker of disease severity in many systemic inflammatory diseases, including ...
Modules of approx. 70 residues. The chitin-binding function has been demonstrated in several cases. These modules are found attached to a number of chitinase catalytic domains, but also in non-catalytic proteins either in isolation or as multiple repeats; chitin binding (EC IIa.chitin ...
Bombyx mori BMCHIR1protein: is a chitinase-like gene induced by 20-Hydroxyecdysone in Silkworm; amino acid sequence in first source
16120DNAArtificial SequenceYM-1 Forward Primer 1tggaattggt gcccctacaa 20220DNAArtificial SequenceYM-1 Reverse Primer 2aacttgcact gtgtatattg 20318DNAArtificial SequenceYM-2 Forward Primer 3aacctcagac attcatta 18421DNAArtificial SequenceYM-2 Reverse Primer 4tggtccttcc agtaggtaat a 21520DNAArtificial SequenceYM-3 Forward Primer 5tataaatctc catttgacac 20620DNAArtificial SequenceYM-3 Reverse Primer 6cctaatttat tgtccttgac 20728DNAArtificial SequenceAMCase Forward Primer 7atctgcagtg gacacacctt catcctga 28828DNAArtificial SequenceAMCase Reverse Primer 8atgaattcaa caagccctgc ttgacaat 28922DNAArtificial SequenceYM Antisense In Situ Hybridization Probe 9tcctcgagac ccagggtact gc 221024DNAArtificial SequenceYM Sense In Situ Hybridization Probe 10tatctagagg atcttcctac cagc 241129DNAArtificial SequenceAMCase Antisense In Situ Hybridization Probe 11tcgctcgaga acaagccctg cttgacaat 291228DNAArtificial SequenceAMCase sense In Situ Hybridization Probe 12gctctagatg gacacacctt catcctga 281319PRTArtificial ...
We found that in adult patients who underwent elective cardiac surgery, UCHI3L1 had inadequate predictive value for CSA-AKI. This was also true for the well-known tubular damage biomarker UNGAL. In contrast, more frequent assessment of the functional biomarker SCr in the early post-operative ICU period (first 4 h) had good to excellent predictive value for CSA-AKI.. Similar to others, our ICU routinely measures SCr at t1 and either around t4 (morning patient) or t5 (afternoon patient) in the early post-operative period. However, with ±50% of AKI diagnosed before t5, of which ±50% before t4, our study highlights the importance of more frequent SCr assessment in the first 4 h. This aids in early AKI diagnosis and could also reveal some cases of rapid reversal of AKI (i.e. complete reversal of AKI by KDIGO criteria within 48 h of AKI onset [30]). These findings are in accordance with those of a small retrospective study (n = 29) by Maciel et al. [31].. Novel findings of our study are the good ...
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012 ...
Chitinase-3-like protein 1 (CHI3L1), also known as YKL-40, is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. The name YKL-40 is derived from the three N-terminal amino acids present on the secreted form and its molecular mass. YKL-40 is expressed and secreted by various cell-types including macrophages, chondrocytes, fibroblast-like synovial cells, vascular smooth muscle cells, and hepatic stellate cells. The biological function of YKL-40 is unclear. It is not known to have a specific receptor. Its pattern of expression is associated with pathogenic processes related to inflammation, extracellular tissue remodeling, fibrosis and solid carcinomas and asthma. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 ...
The rye seed used for substrate is just something along the lines of a perennial rye you use for your lawn? Yes? No? Thanks, Yabecoo
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Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The vector pKLCF-n permits secreted expression of a recombinant protein having a chitin-binding domain (CBD) affinity tag fused to its amino-terminus in the yeast Kluyveromyces lactis . It is compatible with the K
From NCBI Gene:. This gene encodes a member of the CHIC family of proteins. The encoded protein contains a cysteine-rich hydrophobic (CHIC) motif, and is localized to vesicular structures and the plasma membrane. This gene is associated with some cases of acute myeloid leukemia. [provided by RefSeq, Jul 2008]. ...
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Depressed by dieting? Cheer up with chia! Too many high-fat foods, and too little time, make it hard to eat healthy - but chia products can provide a simple, delicious and nutritious solution to diet challenges. Gone are the days when "chia" conjured visions of clay figurines sprouting chia "hair." This ancient Aztec staple has gained increasing recognition as a nutrition powerhouse - and just-published research points to chias potential as an anti-obesity ally.. A basic study from the Journal of Nutritional Biochemistry found that chia significantly restrained unhealthy effects of obesity. Researchers fed rats the equivalent of the American diet - high-sugar, high-fat feed - mixing chia into the chow of a chosen few. After eight weeks, the chia group had 39% lower blood sugar, 25% less stomach fat, 50% less liver fat, and 46% less inflammation than those on the obesity diet alone. Why might this be? Chia is loaded with certain omega-3 fatty acids which may have prompted metabolic enzymes to ...
CHI3L1 antibody to detect human chitinase-3-like protein 1. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
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The Nordic winter sun leaves us with no more than a low lazy light during most of the day. However the few soft winter rays that reach us allow for beautiful pictures with deep colours if you are lucky enough to have your camera at hand and a little time to shoot ...
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2-Furanmethanol, 20-(R)-R, (20S)-protopanaxadiol-3-O-(6-O-malonyl-beta-D-glucopyranosyl(1--,2)-beta-D-glucopyranoside-20-O-beta-D-xylopyranosyl(1--,3)-beta-D-glucopyranosyl(1--,6)-beta-D-glucopyranoside, 20-(S)-Re, 28-glu-oleanolic acid ester, acetylenic alcohol, acidic polysaccharides, adenosine, aglycones, Allheilkraut (German), alpha-maltosyl-beta-D-fructofuranoside, aluminum, American ginseng (AG), American wild ginseng, antioxidants, Araliaceae (family), Asian ginseng, Asian red ginseng, Asiatic ginseng, calcium, capsaicin 4-O-(6-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside, capsaicin 4-O-beta-D-glucoside, ceramide, chikusetsu ginseng, chikusetsusaponin IV, chikusetsusaponin IVa, chikusetsusaponin-L8, chitinolytic enzymes, chosen ninjin, citral, class I chitinase, cobalt, copper, CPPQ (coarse polysaccharide from Panax quinquefolius), CVT-E002, dae-jo-hwan (DJW), dammarane-type glycosides, dammarane-type triterpene ketone (panaxadione), dammarane-type tetracyclic triterpenoid saponins, ...
Statistics based experimental design on chitinase production by Trichoderma harzianum was optimized in solid state fermentation using Plackett-Burman ..
TY - JOUR. T1 - Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects. AU - Uddin, Khan. AU - Orelma, Hannes. AU - Mohammadi, Pezhman. AU - Borghei, Maryam. AU - Laine, Janne. AU - Linder, Markus. AU - Rojas, Orlando. PY - 2017/7/1. Y1 - 2017/7/1. N2 - Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a ...
Plastid transformation technology has been well established and widely utilized in plant transgenic research. In comparison with conventional nuclear gene transformation techniques, plastid engineering offers several potential advantages such as (i) more than 10-100 times greater expression levels than the conventional nuclear transformation system, (ii) a more convenient methodology for transferring multiple genes into plants via gene stacking methods, (iii) elimination of position effects in chloroplasts, which thereby reduces the chances for transgene silencing, (iv) minimal chance for transgene flow by pollen contamination due to maternal inheritance (Verma and Daniell, 2007).. One of the key aspects for the plastid transformation system is to employ a plastidic sequence to exchange exogenous genes into the chloroplast genome via homologous recombination. The usage of genetic markers, which enable the selective enrichment of ptDNA copies, is also a critical component for plastid ...
FANTA, NORA; ORTEGA, XIMENA and PEREZ, LUZ M. The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings. Biol. Res. [online]. 2003, vol.36, n.3-4, pp.411-420. ISSN 0716-9760. http://dx.doi.org/10.4067/S0716-97602003000300012.. In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the ...
Lysozyme Activity in Buffalo Milk: Effect of Lactation Period, Parity, Mastitis, Season in India, pH and Milk Processing Heat Treatment - Lysozyme;Buffalo Milk;Bovine Milk;Parity;Mastitis;
Author summary Fungal pathogens pose an emerging threat in crop production and thus human health. Trichoderma atroviride is considered a potential biocontrol agent against a broad spectrum of phytopathogens. Cell wall chitin was identified as promising target to combat fungal diseases. Here we uncovered the regulation of chitin and chitosan synthesis and their contribution to dynamic cell wall remodeling as protective components in self-defense reactions during the mycoparasitic attack of Trichoderma. The systematic evaluation of the newly identified chitin-modifying enzymes confirmed their concerted interplay and their essential contribution to a successful mycoparasitic invasion. These findings provide further valuable, more specific information on targeting critical factors in the fungal cell wall adaptation process for therapeutic purposes as well as improved biocontrol applications.
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Molecular and immunological characterisation of Acanthocheilonema viteae chitinaseMolecular and immunological characterisation of Acanthocheilonema viteae chitinase

Chitinase purified from inclusion bodies was 13-fold less active compared to soluble chitinase. Synthetic peptides (P1 and P2) ... Vom aktiven Zentrum der Chitinase wurden Peptide synthetisiert und parallel mit Chitinase aus den Einschluss-Körpern für ... Die aus den Einschluss-Körpern aufgereinigte Chitinase zeigte im Vergleich zur löslichen Chitinase eine 13fach verminderte ... were designed from the active site of A. viteae chitinase, and used in parallel with chitinase from inclusion bodies in ...
more infohttps://edoc.hu-berlin.de/handle/18452/16187?C=N

Developmental and functional characterization of cystatin and chitinase of Acanthocheilonema viteaeDevelopmental and functional characterization of cystatin and chitinase of Acanthocheilonema viteae

RNAi of chitinase in adults led to the release of unhatched microfilariae confirming the essential catalytic role of chitinase ... Für die Chitinase von A. viteae konnte eine essentielle Rolle im Häutungsprozess nachgewiesen werden. Das Ausschalten des Gens ... This study also determined that chitinase of A. viteae plays an essential role in the moulting of the L3 larvae since knockdown ... Moreover, maximum expression of chitinase was observed by real-time PCR in the L3 stage supplementing that it is involved in ...
more infohttps://edoc.hu-berlin.de/handle/18452/16281

Chitinase - WikipediaChitinase - Wikipedia

... as well as chitinase-like proteins (such as YKL-40) that have high sequence similarity but lack chitinase activity.[6] ... Chitinase activity can also be detected in human blood[16][17][17] and possibly cartilage.[18] As in plant chitinases this may ... In Saccharomyces cerevisiae and the regulation of ScCts1p (S. cerevisiae chitinase 1), one of the chitinases involved in cell ... Some parts of chitinase molecules, almost identical in structure to hevein or other proteins in rubber latex due to their ...
more infohttps://en.m.wikipedia.org/wiki/Chitinase

chitinase (thing) by BioTech - Everything2.comchitinase (thing) by BioTech - Everything2.com

chitinase (thing). See all of chitinase, no other writeups in this node. ...
more infohttps://everything2.com/user/BioTech/writeups/chitinase

Brugia malayi chitinase (BmCHT1) | NEBBrugia malayi chitinase (BmCHT1) | NEB

Brugia malayi chitinase (BmCHT1) is expressed in the microfilarial stage, the first larval stage, of the organism and is ... Brugia malayi chitinase (BmCHT1) is expressed in the microfilarial stage, the first larval stage, of the organism and is ... Antisera to the chitinase temporarily cleared the microfilaria from the bloodstream of infected jirds (1,4). Product Source. ...
more infohttps://www.neb.com/products/p5205-brugia-malayi-chitinase-bmcht1

Class v chitinase (A0A063BT53) | InterPro | EMBL-EBIClass v chitinase (A0A063BT53) | InterPro | EMBL-EBI

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
more infohttp://www.ebi.ac.uk/interpro/protein/A0A063BT53

Acetazolamide-based fungal chitinase inhibitors.  - PubMed - NCBIAcetazolamide-based fungal chitinase inhibitors. - PubMed - NCBI

Acetazolamide-based fungal chitinase inhibitors.. Schüttelkopf AW1, Gros L, Blair DE, Frearson JA, van Aalten DM, Gilbert IH. ... Here we explore acetazolamide as a chemical scaffold for the inhibition of an A. fumigatus plant-type chitinase. A co-crystal ... The fungal pathogen Aspergillus fumigatus possesses two distinct multi-gene chitinase families. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/21044846?dopt=Abstract

ChitinaseChitinase

... From:. Prior, A.E. Has anyone used chitinases to soften chitin in crustaceans as am having problems with wax ...
more infohttp://www.histosearch.com/histonet/Jun03/Chitinase.html

Chitinase - Proteopedia, life in 3DChitinase - Proteopedia, life in 3D

Role of chitin and chitinase/chitinase-like proteins in inflammation, tissue remodeling, and injury. Annu Rev Physiol. 2011;73: ... The first chitinase structures were solved in 1994, from a bacterium (1ctn) and a plant (2hvm). A mechanism for chitin cleavage ... Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in ... Chitinase A complex with (GlcNac)4 and ethylene glycol (PDB entry 3wh1) ...
more infohttp://proteopedia.org/wiki/index.php/Chitinase

RCSB PDB - Protein Feature View 









 - Chitinase - Q9CE95 (Q9CE95 LACLA)RCSB PDB - Protein Feature View - Chitinase - Q9CE95 (Q9CE95 LACLA)

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
more infohttp://www.rcsb.org/pdb/protein/Q9CE95

Articles citations with the tag: CHITINASEArticles citations with the tag: CHITINASE

Citations with the tag: CHITINASE. Results 1 - 50. *Purification and some properties of a chitinase isozyme from the liver of ... Optimum pH toward colloidal chitin was observed at pH 3.0 for the 38 kDa chitinase, and pH 3.0 and 9.0 for the 42 kDa chitinase ... Acidic mammalian chitinase is not a critical target for allergic airway disease in mice.. Fitz, L. J.; DeClercq, C.; Brooks, J ... Chitinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compound to be excreted onto the hyphae of the ...
more infohttp://connection.ebscohost.com/tag/CHITINASE

Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic AnalysisChondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

... acidic fish chitinase-1: AFCase-1, acidic fish chitinase-2: AFCase-2, fish chitinase-3: FCase-3) in Actinopterygii. In the ... Phylogenetic analysis revealed that Chondrichthyes chitinase forms a unique group (Chondrichthyes chitinase). These results ... Chitinase activity was measured using pNP-(GlcNAc)n (n = 2, 3) as a substrate and β-N-acetylhexosaminidase (Hex) activity was ... Relatively high values of chitinase activity were observed in the stomach, spleen, and gonads of the Japanese bullhead shark, H ...
more infohttps://www.scirp.org/Journal/PaperInformation.aspx?PaperID=82188

RCSB PDB - Protein Feature View 









 - Probable chitinase - Q7NSV3 (Q7NSV3 CHRVO)RCSB PDB - Protein Feature View - Probable chitinase - Q7NSV3 (Q7NSV3 CHRVO)

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
more infohttp://www.rcsb.org/pdb/protein/Q7NSV3

Chitinase for Chitin Hydrolysis - Complex Carbohydrate Analysis | Sigma-AldrichChitinase for Chitin Hydrolysis - Complex Carbohydrate Analysis | Sigma-Aldrich

Chitinase from Streptomyces griseus lyophilized powder (essentially salt free), ≥200 units/g solid * pricing ... Chitinase. EC# 3.2.1.14 Synonyms: 1,4-ß-poly-N-acetylglucosaminidase. Chitinases are enzymes that catalyze the degradation of ...
more infohttps://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=111642814

Chitinase Clostridium Paraputrificum Protein | ProSpecChitinase Clostridium Paraputrificum Protein | ProSpec

Chitinase Clostridium Paraputrificum Recombinant is a non-glycosylated polypeptide chain containing 582 amino acids and having ... Chitinase activity is identified systemically in humans, in the blood, and possibly cartilage. Chitinase has been related to ... Chitinase is a digestive enzyme which breaks down glycosidic bonds in chitin. Due to chitin being a component of the cell walls ... Chitinase expression is mediated by the NPR1 gene and the salicylic acid pathway, both of which are involved in resisting ...
more infohttps://www.prospecbio.com/chitinase

Silencing Chitinase Genes Increases Susceptibility of Tetranychus cinnabarinus (Boisduval) to ScopoletinSilencing Chitinase Genes Increases Susceptibility of Tetranychus cinnabarinus (Boisduval) to Scopoletin

Y. Arakane and S. Muthukrishnan, "Insect chitinase and chitinase-like proteins," Cellular and Molecular Life Sciences, vol. 67 ... J. Zhang, X. Zhang, Y. Arakane et al., "Comparative genomic analysis of chitinase and chitinase-like genes in the African ... Silencing Chitinase Genes Increases Susceptibility of Tetranychus cinnabarinus (Boisduval) to Scopoletin. Hong Zhou, Yong-qiang ... In the present study, 12 full-length cDNAs of chitinase (CHIT) genes from Tetranychus cinnabarinus (designated TcCHITs) were ...
more infohttps://www.hindawi.com/journals/bmri/2017/9579736/

Bacterial Chitinase System as a Model of Chitin Biodegradation | SpringerLinkBacterial Chitinase System as a Model of Chitin Biodegradation | SpringerLink

Bacterial chitinase Bacterial chitinolysis Endo-acting non-processive chitinase Processive chitinase This is a preview of ... Arakane Y, Muthukrishnan S (2010) Insect chitinase and chitinase-like proteins. Cell Mol Life Sci 67:201-216CrossRefGoogle ... Watanabe T, Oyanagi W, Suzuki K, Tanaka H (1990) Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in ... Alam MM, Mizutani T, Isono M, Nikaidou N, Watanabe T (1996) Three chitinase genes (chiA, chiC, and chiD) comprise the chitinase ...
more infohttps://link.springer.com/chapter/10.1007%2F978-981-13-7318-3_7

Chitinase - WikipediaChitinase - Wikipedia

Chitinase at the US National Library of Medicine Medical Subject Headings (MeSH) The X-ray structure of a chitinase from the ... Stress, or environmental signals like ethylene gas, may stimulate increased production of chitinase. Some parts of chitinase ... as well as chitinase-like proteins (such as YKL-40) that have high sequence similarity but lack chitinase activity. Like ... Chitinase activity can also be detected in human blood and possibly cartilage. As in plant chitinases this may be related to ...
more infohttps://en.wikipedia.org/wiki/Chitinase

CHIA - Acidic mammalian chitinase precursor - Bos taurus (Bovine) - CHIA gene & proteinCHIA - Acidic mammalian chitinase precursor - Bos taurus (Bovine) - CHIA gene & protein

IPR011583. Chitinase_II. IPR029070. Chitinase_insertion_sf. IPR001223. Glyco_hydro18_cat. IPR001579. Glyco_hydro_18_chit_AS. ... IPR011583. Chitinase_II. IPR029070. Chitinase_insertion_sf. IPR001223. Glyco_hydro18_cat. IPR001579. Glyco_hydro_18_chit_AS. ... "A novel serum chitinase that is expressed in bovine liver.". Suzuki M., Morimatsu M., Yamashita T., Iwanaga T., Syuto B.. FEBS ... "A novel serum chitinase that is expressed in bovine liver.". Suzuki M., Morimatsu M., Yamashita T., Iwanaga T., Syuto B.. FEBS ...
more infohttp://www.uniprot.org/uniprot/Q95M17

CHITINASE (CAS 9001-6-3) Market Research Report 2018CHITINASE (CAS 9001-6-3) Market Research Report 2018

Market Research Report 2018 aims at providing comprehensive data on chitinase market globally and regionally (Europe, Asia, ... Chitinase prices in North America. 6.4. Chitinase prices in other regions. 7. CHITINASE END-USE SECTOR 7.1. Chitinase market by ... Chitinase market forecast. 6. CHITINASE MARKET PRICES. 6.1. Chitinase prices in Europe. 6.2. Chitinase prices in Asia 6.3. ... 2. CHITINASE APPLICATIONS. 2.1. Chitinase application spheres, downstream products. 3. CHITINASE MANUFACTURING METHODS. 4. ...
more infohttps://marketpublishers.com/report/industry/chemicals_petrochemicals/chitinase_9001-6-3_market_research_report.html

Cloning Expression and Characterization of Chitinase from Bacillus by Mahmood Gillani Madiha | WaterstonesCloning Expression and Characterization of Chitinase from Bacillus by Mahmood Gillani Madiha | Waterstones

Buy Cloning Expression and Characterization of Chitinase from Bacillus by Mahmood Gillani Madiha from Waterstones today! Click ... Cloning Expression and Characterization of Chitinase from Bacillus (Paperback). Mahmood Gillani Madiha (author) Sign in to ...
more infohttps://www.waterstones.com/book/cloning-expression-and-characterization-of-chitinase-from-bacillus/mahmood-gillani-madiha/saleem-faiza/9783659485930

Chitinase-3-like protein 3D structures - Proteopedia, life in 3DChitinase-3-like protein 3D structures - Proteopedia, life in 3D

3D Structures of chitinase-3-like protein. Updated on 07-May-2019 ... Retrieved from "http://proteopedia.org/wiki/index.php/Chitinase-3-like_protein_3D_structures" ...
more infohttp://proteopedia.org/wiki/index.php/Chitinase-3-like_protein_3D_structures

A Small RNA Controls Expression of the Chitinase ChiA in Listeria monocytogenesA Small RNA Controls Expression of the Chitinase ChiA in Listeria monocytogenes

In recent years, more than 60 small RNAs (sRNAs) have been identified in the gram-positive human pathogen Listeria monocytogenes, but their putative roles and mechanisms of action remain largely unknown. The sRNA LhrA was recently shown to be a post-transcriptional regulator of a single gene, lmo0850, which encodes a small protein of unknown function. LhrA controls the translation and degradation of the lmo0850 mRNA by an antisense mechanism, and it depends on the RNA chaperone Hfq for efficient binding to its target. In the present study, we sought to gain more insight into the functional role of LhrA in L. monocytogenes. To this end, we determined the effects of LhrA on global-wide gene expression. We observed that nearly 300 genes in L. monocytogenes are either positively or negatively affected by LhrA. Among these genes, we identified lmo0302 and chiA as direct targets of LhrA, thus establishing LhrA as a multiple target regulator. Lmo0302 encodes a hypothetical protein with no known function,
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019019

3CHC: Crystal Structure Of Aspergillus Fumigatus Chitinase B1 In Complex With Monopeptide - NCBI structure3CHC: Crystal Structure Of Aspergillus Fumigatus Chitinase B1 In Complex With Monopeptide - NCBI structure

Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product ... The peptide fragments inhibit chitinase B1 from Aspergillus fumigatus (AfChiB1), the human chitotriosidase, and chitinase ... Structure-based dissection of the natural product cyclopentapeptide chitinase inhibitor argifin. Andersen OA, Nathubhai A, ... Chitinase inhibitors have chemotherapeutic potential as fungicides, pesticides, and antiasthmatics. Argifin, a natural product ...
more infohttps://www.ncbi.nlm.nih.gov/Structure/pdb/3CHC

Cloning chitinase genes from Plasmodiophora brassicae into Escherichia coliCloning chitinase genes from Plasmodiophora brassicae into Escherichia coli

For this end, methods for RNA extraction, followed by PCR, cloning, and chitinase activity testing were used.Complete enzymes ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:1199931
  • Vom aktiven Zentrum der Chitinase wurden Peptide synthetisiert und parallel mit Chitinase aus den Einschluss-Körpern für Vakzinierungsexperimente im A. viteae / Meriones unguiculatus-Filarien-Modell genutzt. (hu-berlin.de)
  • Die Moleküle Cystatin und Chitinase wurden, auf Grund ihrer immunodulatorischen und katalytischen Eigenschaften, bei der Nager-Filarie Acanthocheilonema viteae als essentielle Proteine identifiziert. (hu-berlin.de)
  • Mittels RNAi bei adulten Parasiten konnte die katalytische Rolle der Chitinase beim Abbau des Chitins im Ei bestätigt werden, da hier nur ungeschlüpfte Mikrofilarien ausgeschieden wurden. (hu-berlin.de)
  • The prototypic chitinase-like protein chitinase 3-like-1 (CHI3L1) plays a protective role in the lung by ameliorating cell death and stimulating fibroproliferative repair. (jci.org)
  • A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and. (ebscohost.com)
  • An abstract of an article about mammalian cells that ectopically produced homologous chitinase-like proteins, CHI3L1 and CHI3L2, is presented and mentions topics including gliomas, cell proliferation, and cell growth. (ebscohost.com)
  • Chitinase-3-like 1 (CHI3L1) is a glycoprotein elevated in paediatric severe malaria, and an emerging urinary biomarker of acute kidney injury (AKI). (springer.com)
  • For example, two or more kinds of chitinase isozymes have been discovered in the stomach of Actinopterygii, one of the most successful vertebrates on earth. (scirp.org)
  • Chitinase Clostridium Paraputrificum Recombinant fused with a His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 582 amino acids and having a molecular mass of 64.2kDa. (prospecbio.com)
  • CHITINASE (CAS 9001-6-3) Market Research Report 2018 aims at providing comprehensive data on chitinase market globally and regionally (Europe, Asia, North America, Latin America etc. (marketpublishers.com)
  • CHITINASE (CAS 9001-6-3) Market Research Report 2018 contents were worked out and placed on the website in March, 2018. (marketpublishers.com)
  • Please note that CHITINASE (CAS 9001-6-3) Market Research Report 2018 is a half ready publication and contents are subject to change. (marketpublishers.com)
  • The first chitinase structures were solved in 1994, from a bacterium ( 1ctn ) and a plant ( 2hvm ). (proteopedia.org)
  • The aforementioned points are a clear indication of the potential for the application of chitin in a wide range of fields and the research on chitinase enzymes was invaluable to their efficient use. (scirp.org)
  • Berger LR, Reynolds DM (1958) The chitinase system of a strain of Streptomyces griseus . (springer.com)
  • Here we explore acetazolamide as a chemical scaffold for the inhibition of an A. fumigatus 'plant-type' chitinase. (nih.gov)
  • Purification and some properties of a chitinase isozyme from the liver of Japanese common squid Todarodes pacificus. (ebscohost.com)
  • Describes the purification and properties of chitinase isozyme from the liver of Japanese common squid Todarodes pacificus. (ebscohost.com)
  • Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. (proteopedia.org)
  • Mechanistically, the role of CF airway proteases and genetic chitinase deficiency was assessed. (uni-muenchen.de)
  • The optimal pH of chitinase in both the Japanese bullhead shark, H. japonicas , and the Kwangtung skate, D. kwangtungensis , were 3.5 - 5.5 and 3.5 - 4.0, respectively, and 4.0 for Hex in both species. (scirp.org)
  • These results suggested that the possibility of the formation of chitinase groups for each class in the phylogenetic analysis based on the observation of class-specific chitinase. (scirp.org)
  • About 80% of the expressed chitinase were found in inclusion bodies and were purified under denaturing conditions. (hu-berlin.de)
  • Phylogenetic analysis revealed that Chondrichthyes chitinase forms a unique group (Chondrichthyes chitinase). (scirp.org)
  • X-ray crystallographic analysis of the chitinase-inhibitor complexes revealed that the conformations of the linear peptides were remarkably similar to that of the natural product. (nih.gov)