Chimerism: The occurrence in an individual of two or more cell populations of different chromosomal constitutions, derived from different individuals. This contrasts with MOSAICISM in which the different cell populations are derived from a single individual.Transplantation Chimera: An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.Chimera: An individual that contains cell populations derived from different zygotes.Transplantation Tolerance: An induced state of non-reactivity to grafted tissue from a donor organism that would ordinarily trigger a cell-mediated or humoral immune response.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Transplantation Conditioning: Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.Graft Survival: The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.Skin Transplantation: The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Whole-Body Irradiation: Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.Graft vs Host Disease: The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.Host vs Graft Reaction: The immune responses of a host to a graft. A specific response is GRAFT REJECTION.Radiation Chimera: An organism whose body contains cell populations of different genotypes as a result of the TRANSPLANTATION of donor cells after sufficient ionizing radiation to destroy the mature recipient's cells which would otherwise reject the donor cells.Immune Tolerance: The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.Lymphocyte Transfusion: The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.Transplantation Immunology: A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.Leukocyte Transfusion: The transfer of leukocytes from a donor to a recipient or reinfusion to the donor.Histocompatibility Testing: Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)Tissue Donors: Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.Graft Rejection: An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.Lymphocyte Depletion: Immunosuppression by reduction of circulating lymphocytes or by T-cell depletion of bone marrow. The former may be accomplished in vivo by thoracic duct drainage or administration of antilymphocyte serum. The latter is performed ex vivo on bone marrow before its transplantation.Busulfan: An alkylating agent having a selective immunosuppressive effect on BONE MARROW. It has been used in the palliative treatment of chronic myeloid leukemia (MYELOID LEUKEMIA, CHRONIC), but although symptomatic relief is provided, no permanent remission is brought about. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), busulfan is listed as a known carcinogen.Hematologic Neoplasms: Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.Vidarabine: A nucleoside antibiotic isolated from Streptomyces antibioticus. It has some antineoplastic properties and has broad spectrum activity against DNA viruses in cell cultures and significant antiviral activity against infections caused by a variety of viruses such as the herpes viruses, the VACCINIA VIRUS and varicella zoster virus.Immunosuppressive Agents: Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.Graft Enhancement, Immunologic: The induction of prolonged survival and growth of allografts of either tumors or normal tissues which would ordinarily be rejected. It may be induced passively by introducing graft-specific antibodies from previously immunized donors, which bind to the graft's surface antigens, masking them from recognition by T-cells; or actively by prior immunization of the recipient with graft antigens which evoke specific antibodies and form antigen-antibody complexes which bind to the antigen receptor sites of the T-cells and block their cytotoxic activity.Cord Blood Stem Cell Transplantation: Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Lymphocyte Culture Test, Mixed: Measure of histocompatibility at the HL-A locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way MLC test, the lymphocytes from one of the individuals are inactivated (usually by treatment with MITOMYCIN or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens.Myeloablative Agonists: Agents that destroy bone marrow activity. They are used to prepare patients for BONE MARROW TRANSPLANTATION or STEM CELL TRANSPLANTATION.Histocompatibility: The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.Tandem Repeat Sequences: Copies of DNA sequences which lie adjacent to each other in the same orientation (direct tandem repeats) or in the opposite direction to each other (INVERTED TANDEM REPEATS).Y Chromosome: The male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans and in some other male-heterogametic species in which the homologue of the X chromosome has been retained.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).CD40 Ligand: A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.Freemartinism: A condition occurring in the female offspring of dizygotic twins (TWIN, DIZYGOTIC) in a mixed-sex pregnancy, usually in CATTLE. Freemartinism can occur in other mammals. When placental fusion between the male and the female FETUSES permits the exchange of fetal cells and fetal hormones, TESTICULAR HORMONES from the male fetus can androgenize the female fetus producing a sterile XX/XY chimeric "female"(CHIMERISM).Immunosuppression: Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.Rats, Inbred ACIGraft vs Tumor Effect: Immunological rejection of tumor tissue/cells following bone marrow transplantation.Hemibody Irradiation: Irradiation of one half or both halves of the body in the treatment of disseminated cancer or widespread metastases. It is used to treat diffuse metastases in one session as opposed to multiple fields over an extended period. The more frequent treatment modalities are upper hemibody irradiation (UHBI) or lower hemibody irradiation (LHBI). Less common is mid-body irradiation (MBI). In the treatment of both halves of the body sequentially, hemibody irradiation permits radiotherapy of the whole body with larger doses of radiation than could be accomplished with WHOLE-BODY IRRADIATION. It is sometimes called "systemic" hemibody irradiation with reference to its use in widespread cancer or metastases. (P. Rubin et al. Cancer, Vol 55, p2210, 1985)Mice, Inbred C57BLLeukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Isoantigens: Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Blood Grouping and Crossmatching: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Mice, Inbred BALB CMosaicism: The occurrence in an individual of two or more cell populations of different chromosomal constitutions, derived from a single ZYGOTE, as opposed to CHIMERISM in which the different cell populations are derived from more than one zygote.Organ Transplantation: Transference of an organ between individuals of the same species or between individuals of different species.Chromosomes, Human, Y: The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.Blood Cells: The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.HLA Antigens: Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.Models, Animal: Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Mice, Inbred NOD: A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.Transplantation, Isogeneic: Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.Allografts: Tissues, cells, or organs transplanted between genetically different individuals of the same species.Antilymphocyte Serum: Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.Islets of Langerhans Transplantation: The transference of pancreatic islets within an individual, between individuals of the same species, or between individuals of different species.Fetal Therapies: Prenatal interventions to correct fetal anomalies or treat FETAL DISEASES in utero. Fetal therapies include several major areas, such as open surgery; FETOSCOPY; pharmacological therapy; INTRAUTERINE TRANSFUSION; STEM CELL TRANSPLANTATION; and GENETIC THERAPY.Minisatellite Repeats: Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Neoplasm, Residual: Remnant of a tumor or cancer after primary, potentially curative therapy. (Dr. Daniel Masys, written communication)Lymphatic Irradiation: External or interstitial irradiation to treat lymphomas (e.g., Hodgkin's and non-Hodgkin's lymphomas) and lymph node metastases and also some autoimmune diseases, such as rheumatoid arthritis.Heart Transplantation: The transference of a heart from one human or animal to another.Spleen: An encapsulated lymphatic organ through which venous blood filters.Saguinus: A genus in the subfamily CALLITRICHINAE consisting of 12 species and found in Panama as well as South America. Species seen most frequently in the literature are S. oedipus (cotton-top marmoset), S. nigricollis, and S. fusicollis.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Recurrence: The return of a sign, symptom, or disease after a remission.Leukemia, Myelogenous, Chronic, BCR-ABL Positive: Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.Severe Combined Immunodeficiency: Group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. It is inherited as an X-linked or autosomal recessive defect. Mutations occurring in many different genes cause human Severe Combined Immunodeficiency (SCID).Peripheral Tolerance: The mechanism, in peripheral lymphoid organs (LYMPH NODES; SPLEEN; TONSILS; and mucosal-associated lymphoid tissue), that prevents mature lymphocytes from reacting to SELF-ANTIGENS. This is accomplished through a variety of means including CLONAL ANERGY and CLONAL DELETION.Graft vs Host Reaction: An immunological attack mounted by a graft against the host because of tissue incompatibility when immunologically competent cells are transplanted to an immunologically incompetent host; the resulting clinical picture is that of GRAFT VS HOST DISEASE.Time Factors: Elements of limited time intervals, contributing to particular results or situations.beta-Thalassemia: A disorder characterized by reduced synthesis of the beta chains of hemoglobin. There is retardation of hemoglobin A synthesis in the heterozygous form (thalassemia minor), which is asymptomatic, while in the homozygous form (thalassemia major, Cooley's anemia, Mediterranean anemia, erythroblastic anemia), which can result in severe complications and even death, hemoglobin A synthesis is absent.Kidney Transplantation: The transference of a kidney from one human or animal to another.Hydrozoa: A class in the phylum CNIDARIA which alternates between polyp and medusa forms during their life cycle. There are over 2700 species in five orders.Clonal Deletion: Removal, via CELL DEATH, of immature lymphocytes that interact with antigens during maturation. For T-lymphocytes this occurs in the thymus and ensures that mature T-lymphocytes are self tolerant. B-lymphocytes may also undergo clonal deletion.Transplantation, Heterologous: Transplantation between animals of different species.Mice, Inbred C3HMajor Histocompatibility Complex: The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Twins, Dizygotic: Two offspring from the same PREGNANCY. They are from two OVA, fertilized at about the same time by two SPERMATOZOA. Such twins are genetically distinct and can be of different sexes.Blood Transfusion, Intrauterine: In utero transfusion of BLOOD into the FETUS for the treatment of FETAL DISEASES, such as fetal erythroblastosis (ERYTHROBLASTOSIS, FETAL).Graft vs Leukemia Effect: Immunological rejection of leukemia cells following bone marrow transplantation.Thymus Gland: A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.Transplants: Organs, tissues, or cells taken from the body for grafting into another area of the same body or into another individual.Amelogenin: A major dental enamel-forming protein found in mammals. In humans the protein is encoded by GENES found on both the X CHROMOSOME and the Y CHROMOSOME.Reticulocyte Count: The number of RETICULOCYTES per unit volume of BLOOD. The values are expressed as a percentage of the ERYTHROCYTE COUNT or in the form of an index ("corrected reticulocyte index"), which attempts to account for the number of circulating erythrocytes.ABO Blood-Group System: The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.Blood DonorsCell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.Anemia, Aplastic: A form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements.Mice, Inbred CBATreatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Peripheral Blood Stem Cell Transplantation: Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Callithrix: A genus of the subfamily CALLITRICHINAE occurring in forests of Brazil and Bolivia and containing seventeen species.Mycophenolic Acid: An antibiotic substance derived from Penicillium stoloniferum, and related species. It blocks de novo biosynthesis of purine nucleotides by inhibition of the enzyme inosine monophosphate dehydrogenase. Mycophenolic acid is important because of its selective effects on the immune system. It prevents the proliferation of T-cells, lymphocytes, and the formation of antibodies from B-cells. It also may inhibit recruitment of leukocytes to inflammatory sites. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1301)Cyclosporine: A cyclic undecapeptide from an extract of soil fungi. It is a powerful immunosupressant with a specific action on T-lymphocytes. It is used for the prophylaxis of graft rejection in organ and tissue transplantation. (From Martindale, The Extra Pharmacopoeia, 30th ed).Cyclophosphamide: Precursor of an alkylating nitrogen mustard antineoplastic and immunosuppressive agent that must be activated in the LIVER to form the active aldophosphamide. It has been used in the treatment of LYMPHOMA and LEUKEMIA. Its side effect, ALOPECIA, has been used for defleecing sheep. Cyclophosphamide may also cause sterility, birth defects, mutations, and cancer.Hematologic Diseases: Disorders of the blood and blood forming tissues.Histocompatibility Antigens: A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.Cell Transplantation: Transference of cells within an individual, between individuals of the same species, or between individuals of different species.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Blood Group Incompatibility: An antigenic mismatch between donor and recipient blood. Antibodies present in the recipient's serum may be directed against antigens in the donor product. Such a mismatch may result in a transfusion reaction in which, for example, donor blood is hemolyzed. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984).Cytogenetics: A subdiscipline of genetics which deals with the cytological and molecular analysis of the CHROMOSOMES, and location of the GENES on chromosomes, and the movements of chromosomes during the CELL CYCLE.Genes, sry: The primary testis-determining gene in mammalians, located on the Y CHROMOSOME. It codes for a high mobility group box transcription factor (TRANSCRIPTION FACTORS) which initiates the development of the TESTES from the embryonic GONADS.Thalassemia: A group of hereditary hemolytic anemias in which there is decreased synthesis of one or more hemoglobin polypeptide chains. There are several genetic types with clinical pictures ranging from barely detectable hematologic abnormality to severe and fatal anemia.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.Granulomatous Disease, Chronic: A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Immunoconjugates: Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.Vascularized Composite Allotransplantation: Transference of multiple tissues, such as muscle, bone, nerve, and skin, as a functional unit for reconstructive purposes. Blood supply to the transplanted tissues is maintained throughout the transplantation procedure with minimal ischemia. Maintenance of vascularity in the graft promotes its viability, function, and survival in the recipient.Fetal Diseases: Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.Parabiosis: The experimental joining of two individuals for the purpose of studying the effects of one on the other.Callitrichinae: A subfamily in the family CEBIDAE that consists of four genera: CALLITHRIX (marmosets), CALLIMICO (Goeldi's monkey), LEONTOPITHECUS (lion tamarins), and SAGUINUS (long-tusked tamarins). The members of this family inhabit the tropical forests of South and Central America.

Revisiting liver transplant immunology: from the concept of immune engagement to the dualistic pathway paradigm. (1/276)

Ever since the demonstration that allografts are rejected through immune reactions of the host, clinical therapies for organ allografts have relied on immune suppression to prevent these destructive events. A growing body of clinical and experimental data suggests that allografts elicit multiple, interactive immune responses. The result is not inevitably graft rejection, and "spontaneous" acceptance of fully allogeneic liver grafts occurs in rodents without immunosuppression. A spectrum of results range from spontaneous acceptance without immunosuppression to rejection with immunosuppression. The "dualistic pathway paradigm" aims to reconcile apparently conflicting observations in liver transplantation and proposes that: (1) immune engagement between the host and the allograft is instrumental in both rejection and acceptance; (2) there exist in all mammalian species congruent interactive pathways of immune activation whereby the fate of the allograft is determined by the quantitative results of these interactions; (3) the dualistic effect of immunosuppressive drugs on pathways of immune activation, conferring the capacity for favorable or unfavorable graft outcome should be investigated in experimental models in which organ allografts are spontaneously accepted. In conclusion the design of clinical strategies based on this research may contribute to protocols resulting in allograft acceptance without chronic immunosuppression.  (+info)

Living donor liver transplant with clinical tolerance, laboratory evidence of chimerism, and spontaneous clearance of HBV. (2/276)

We present a case of functional and histopathologic tolerance, chimerism, and spontaneous clearance of HBV in a patient four years after living donor liver transplant (LDLT). A 19-year-old male patient underwent a LDLT for HBV cirrhosis. He voluntarily ceased immunosuppression and antiviral therapy after 6 months. He is now four years status post transplant without any episodes of rejection or clinical manifestation of liver disease. PCR and VNTR were used to show donor-recipient chimerism and a large degree of genetic similarity between the pair. MLC and cytokine elaboration were used to show recipient hyporeactivity towards donor antigen. He also has clinical evidence of clearing his HBV without continued use of HBIG.  (+info)

Maternal and sibling microchimerism in twins and triplets discordant for neonatal lupus syndrome-congenital heart block. (3/276)

OBJECTIVE: Neonatal lupus syndrome-congenital heart block (NLS-CHB) is an acquired autoimmune disease in which maternal autoantibodies are necessary but not sufficient for disease. Maternal myocardial cells have been found in the hearts of patients with NLS-CHB, suggesting that maternal microchimerism may also play a role. In this study we asked whether levels of microchimerism in the blood are associated with NLS-CHB in discordant twins and triplets. METHODS: Human leucocyte antigen (HLA)-specific and Y-chromosome-specific real-time quantitative polymerase chain reaction (PCR) was used to quantitatively assay maternal and sibling microchimerism in peripheral blood. Because of HLA allele sharing in families, it was not always possible to distinguish between multiple sources of microchimerism. RESULTS: In one family, maternal and/or sibling microchimerism was detected in two triplets who had CHB, but not in the triplet with transient hepatitis. Levels ranged from 4 to 948 genome-equivalents of foreign deoxyribonucleic acid per million host genome-equivalents (gEq/million). Over the first year levels of sibling microchimerism decreased in the triplet with complete CHB and increased in the triplet who progressed from first- to second-degree CHB. In a second family, maternal and/or sibling microchimerism was detected in the healthy twin (1223 gEq/million) but not in the twin with CHB. CONCLUSIONS: Maternal and/or sibling microchimerism was detectable in the blood of infant twins and triplets discordant for NLS. Microchimerism in the blood was not specific for NLS-CHB, although in one family levels correlated with disease. Thus, microchimerism in the blood and/or tissues may be involved in the pathogenesis or progression of NLS-CHB, but additional factors must also contribute. Further investigation is warranted.  (+info)

Male microchimerism in women with systemic sclerosis and healthy women who have never given birth to a son. (4/276)

BACKGROUND: Male DNA or cells are often used to measure microchimerism in a woman. In studies of autoimmune diseases male microchimerism is most often attributed to the previous birth of a son. OBJECTIVE: To determine the frequency of male microchimerism in healthy women or women with systemic sclerosis who had never given birth to a son. METHODS: Real time quantitative polymerase chain reaction targeting the Y chromosome specific sequence DYS14 was employed to test DNA extracted from peripheral blood mononuclear cells of 26 women with systemic sclerosis and 23 healthy women who had never given birth to a son. RESULTS: are expressed as the genome equivalent number of male cells per million host cells (gEq/mil).Results: Male DNA was found in 15% of women with systemic sclerosis (range 0 to 23.7 gEq/mil) and in 13% of healthy women (range 0 to 5.1 gEq/mil). Although two women with male DNA had an induced abortion, most had no history of spontaneous or induced abortion (either systemic sclerosis or healthy). CONCLUSIONS: Microchimerism with male DNA can be found in the circulation of women who have never given birth to a son. Thus sources other than a male birth must be considered when male DNA is used to measure microchimerism. Although other studies are needed, there was no apparent difference in women with systemic sclerosis and healthy women. Possible sources of male DNA include unrecognised male pregnancy or unrecognised male twin, an older male sibling with transfer through the maternal circulation, or sexual intercourse alone.  (+info)

Emergent autoimmunity in graft-versus-host disease. (5/276)

Donor T-cell recognition of host alloantigens presented by host antigen-presenting cells (APCs) is necessary for the induction of graft-versus-host disease (GVHD), but whether direct alloreactivity is sufficient for the propagation of GVHD is unknown. In this study, we demonstrate that GVHD cannot be effectively propagated through the direct pathway of allorecognition. Rather, donor T-cell recognition of antigens through the indirect pathway is necessary for the perpetuation of GVHD. Furthermore, GVHD results in the breaking of self tolerance, resulting in the emergence of donor T cells that can cause autoimmune disease in syngeneic recipients. Notably, GVHD-induced autoreactivity is donor APC dependent, transferable into secondary hosts, and involves cells of the innate immune system. These results indicate that donor T-cell--mediated pathologic damage during GVHD becomes donor APC dependent and provide a mechanistic explanation for the long-standing observation that GVHD is associated with autoimmune clinical manifestations.  (+info)

Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation. (6/276)

Autosomal dominant polycystic kidney disease (ADPKD) is the most common human monogenic genetic disorder and is characterized by progressive bilateral renal cysts and the development of renal insufficiency. The cystogenesis of ADPKD is believed to be a monoclonal proliferation of PKD-deficient (PKD(-/-)) renal tubular epithelial cells. To define the function of Pkd1, we generated chimeric mice by aggregation of Pkd1(-/-) ES cells and Pkd1(+/+) morulae from ROSA26 mice. As occurs in humans with ADPKD, these mice developed cysts in the kidney, liver, and pancreas. Surprisingly, the cyst epithelia of the kidney were composed of both Pkd1(-/-) and Pkd1(+/+) renal tubular epithelial cells in the early stages of cystogenesis. Pkd1(-/-) cyst epithelial cells changed in shape from cuboidal to flat and replaced Pkd1(+/+) cyst epithelial cells lost by JNK-mediated apoptosis in intermediate stages. In late-stage cysts, Pkd1(-/-) cells continued immortalized proliferation with downregulation of p53. These results provide a novel understanding of the cystogenesis of ADPKD patients. Furthermore, immortalized proliferation without induction of p53 was frequently observed in 3T3-type culture of mouse embryonic fibroblasts from Pkd1(-/-) mice. Thus, Pkd1 plays a role in preventing immortalized proliferation of renal tubular epithelial cells through the induction of p53 and activation of JNK.  (+info)

Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse. (7/276)

Fetal cells circulate in pregnant women and persist in blood and tissue for decades post-partum. The mother thus becomes chimeric. Factors that may influence such fetal cell microchimerism include histocompatibility, fetal or placental abnormalities, or a reproductive history that includes miscarriage or elective termination. Fetal cell microchimerism is associated with some maternal autoimmune diseases, such as systemic sclerosis. Moreover, a novel population of fetal cells, the pregnancy-associated progenitor cells (PAPCs), appears to differentiate in diseased or injured maternal tissue. The cellular origin of these cells is at present unknown but could be a hematopoietic stem cell, a mesenchymal stem cell, or a novel cell type. Pregnancy therefore results in the acquisition of cells with stem-cell-like properties that may influence maternal health post-partum. Rather than triggering disease, these cells may instead combat it.  (+info)

Chimerism in kidneys, livers and hearts of normal women: implications for transplantation studies. (8/276)

Tissue chimerism was recently described in transplanted organs from female donors into male recipients, by demonstration of the Y-chromosome in tissue-derived cells. It was claimed that these Y-chromosome positive cells were recipient derived. To find out whether the chimeric cells, derived from pregnancies of sons or blood transfusions, could have been present in the solid organs before transplantation, we performed the following study. In situ hybridization for the Y-chromosome was performed on the normal organs (51 kidneys, 51 livers, 69 hearts) from 75 women of the normal population, whose child and blood transfusion status were known. Chimeric cells were found in 13 kidneys, 10 livers and 4 hearts, of 23 women. There was no relation between the child status or the blood transfusion history with the presence of Y-chromosome positive cells. We have for the first time demonstrated that male cells are present in normal kidneys, livers and hearts. Theoretically, these organs could have been used for the transplantation. Therefore, our findings demonstrate that the chimeric cells thus far described in transplantation studies, are not necessarily donor derived, and could have been present in the organs before the transplantation.  (+info)

*Tandem chimerism

Parra et al., Tandem Chimerism as a means to increase protein complexity in the human genome Parra et al., Tandem chimerism as ... Tandem chimerism is the phenomenon where two consecutive genes are transcribed into a single RNA molecule. The translation ( ...

*Genetic chimerism in fiction

Human genetic chimerism, which can not only cause a wide range of illnesses but also lead to the same person having more than ... In Mother 3, chimerism is one of main themes, with chimeric animals being introduced early on in the game. The first chimera ... In the House episode "Cane and Able", a boy who believes he was abducted by aliens is diagnosed with chimerism. In an episode ... One of the subplots in Michael Crichton's 2006 novel Next deals with chimerism. In season three of the TV series ReGenesis, a ...

*Chimera (genetics)

Tetragametic chimerism is a form of congenital chimerism. This condition occurs through the fertilisation of two separate ova ... Chimerism Explained Chimerism and cellular mosaicism, Genetic Home Reference, U.S. National Library of Medicine, National ... Cases of human chimerism have been documented. This condition is either inherited or it is acquired through the infusion of ... Germline chimerism occurs when the germ cells (for example, sperm and egg cells) of an organism are not genetically identical ...

*Microchimerism

Chimerism, 6(4), 65-71. A. Fujishiro, K. Kawakura, Y-I. Miyake, Y. Kaneda, "A fast, convenient diagnosis of the bovine ... Chimerism Allotransplantation Telegony Epigenetics Bianchi, DW; Zickwold GK; Weil GJ; Sylvester S; DeMaria MA. (1996). "Male ... Chimerism. 4 (4): 111-118. doi:10.4161/chim.25055. PMC 3921191 . Tan, Xiao-Wei; Liao, Hong; Sun, Li; Okabe, Masaru; Xiao, Zhi- ... also known as fetal cell microchimerism or fetal chimerism) whereby cells from a fetus pass through the placenta and establish ...

*Wied's marmoset

Researchers first discovered chimerism in the bone marrow of marmosets in the 1960s. More recent work has shown that chimerism ... This chimerism is the result of cell lines exchanged between siblings in utero. These two original zygotes were fertilized by ... Since chimerism changes the degrees of relatedness between individuals, it also changes the adaptive value of certain behaviors ... Ross, C.N.; French, J.A. & Ortí, G. (2007). "Germ-line chimerism and paternal care in marmosets (Callithrix kuhlii)". Proc. ...

*Horse markings

Not to be confused with the zebra, which is an entirely different species.) Brindling may be associated with chimerism. Dun: A ...

*Mixed-field agglutination

By far the most common cause of mixed-field agglutination is false chimerism. There are several causes of false chimerism; 1) ... 3) Weak expression of the A or B blood group antigen 4) In some diseases, for example leukaemia A true chimerism is a rare ... Twin chimerism results from mixing of blood between two twin fetuses through placental blood vessel anastomoses, leading to ... The presence of two or more cell populations is known as chimerism. Mixed-field agglutination is an important cause of ABO ...

*46, XX/46,XY

However, this trait is not unique to 46,XX/46,XY chimerism. It has also been observed in other types of chimerism. Individuals ... The chimerism arises in utero from the combination of an XX zygote and an XY zygote (which otherwise would have developed into ... 46,XX/46,XY is an example of tetragametic chimerism because it requires four gametes - two sperm and two ova. 46,XX/46,XY is ... 46,XX/46,XY chimerism can be identified during pregnancy by prenatal screening or in early childhood through genetic testing ...

*Lydia Fairchild

... is an American woman who exhibits chimerism, in having two distinct populations of DNA among the cells of her ... He realised that Fairchild's case might also be caused by chimerism. As in Keegan's case, DNA samples were taken from members ... Fairchild was carrying two different sets of DNA, the defining characteristic of chimerism. Mater semper certa est Yu, Neng; et ... 16 May 2002). "Disputed Maternity Leading to Identification of Tetragametic Chimerism". New England Journal of Medicine. 346 ( ...

*Genetically modified organism

2017). "Interspecies Chimerism with Mammalian Pluripotent Stem Cells". Cell. 168 (3): 473-86. doi:10.1016/j.cell.2016.12.036. ...

*Sheep-goat chimera

Fehilly, Carole B.; Willadsen, S. M.; Tucker, Elizabeth M. (16 February 1984). "Interspecific chimaerism between sheep and goat ...

*Marmoset

Ross, C.N., French, J.A., and Ortí, G. (2007). "Germ-line chimerism and paternal care in marmosets (Callithrix kuhlii)". Proc. ... According to recent research, marmosets exhibit germline chimerism, which is not known to occur in nature in any primates other ... Gengozian, N.; Batson, JS; Eide, P. (1964). "Hematologic and Cytogenetic Evidence for Hematopoietic Chimerism in the Marmoset, ...

*Yeast artificial chromosome

Chimerism may be due to either co-ligation of multiple genomic segments into a single YAC, or recombination of two or more YACs ... The incidence of chimerism may be as high as 50%. Other artifacts are deletion of segments from a cloned region, and ... "Large human YACs constructed in a rad52 strain show a reduced rate of chimerism". Genomics. 24 (3): 478-84. doi:10.1006/geno. ...

*Mythology of Teen Wolf

She has chimerism due to her having an allogeniac skin graft. Tracy was the first Chimera in the Dread Doctors' newest ... Mason had genetic chimerism due to his being born with vanishing twin syndrome. Naturally and inherently good-hearted, kind and ... Chimerism can occur in humans by either organ transplantation or vanishing twin syndrome. The mythological creature known as ... The test subjects had to have genetic chimerism or organs from outside donors. The latest chimera test subject, if successful, ...

*BRT Laboratories

Test offerings include Tuberculosis testing and Bone Marrow Engraftment (chimerism) monitoring. BRT's tuberculosis (TB) testing ...

*Freemartin

Mosaicism and Chimerism at colostate.edu Picture at agric.gov.ab.ca. ...

*Equine coat color

Brindle: One of the rarest colors in horses, possibly linked to chimerism. Characteristics are any color with "zebra-like" ...

*Non-communicable disease

An increase in the amount of genetic information, such as Chimerism or Heterochromia. Cystic fibrosis is an example of an ...

*Gene

"Tandem chimerism as a means to increase protein complexity in the human genome". Genome Research. 16 (1): 37-44. doi:10.1101/gr ...

*Conjoined gene

January 2006). "Tandem chimerism as a means to increase protein complexity in the human genome". Genome Research. 16 (1): 37-44 ...

*Heterochromia iridum

... chimerism, disease, or injury. Heterochromia of the eye (heterochromia iridum or heterochromia iridis) is of three kinds. In ...

*Twin

... chimerism and twinning". Human Reproduction. Oxford Journals. 18 (2): 236-242. doi:10.1093/humrep/deg060. PMID 12571155. ...

*Xenotransplantation

A proposed strategy to avoid cellular rejection is to induce donor non-responsiveness using hematopoietic chimerism. Donor stem ...

*Wish I May (TV series)

However, due to the presence of her strange illness, chimerism, she will constantly deny her own daughter. in the midst of ...

*David Sachs

In the 1980s Sachs had shown that mixed-chimerism can lead to transplant tolerance in small animal models; these findings were ...
Chimerism analysis is one part of the monitoring process for bone marrow transplant patients. The pre-transplant chimerism analysis of both donor and recipient is necessary in order to evaluate for the presence of hematopoietic microchimerism in patients at various time points subsequent to allogeneic bone marrow transplant. Hematopoietic microchimerism will be determined by PCR analysis using a panel of highly-polymorphic short tandem repeat (STR) markers. DNA will be extracted from the recipients peripheral blood cells or bone marrow. The number of alleles in the profile established by the STR panel at the time of the pre-transplant chimerism analysis will determine the complexity of the post-transplant chimerism analysis. Screen-informative STRs from post-transplant recipient samples will be quantified to determine the percent of hematopoietic microchimerism in the patient.. ...
The role of interleukin-2 (IL-2) in thymic development is uncertain. Not surprisingly, IL-2 knockout (KO) mice have been used to address this question. However, as we report here, such mice are chimeric, containing both IL-2 KO cells and IL-2-expressing cells transferred in utero from their heterozygous mothers. These cells produce IL-2 in amounts detectable by conventional means, and their presence in lymphoid tissues confounds efforts to define the true IL-2 KO phenotype. To minimize the amount of IL-2 available to the thymus, we subjected recombinase activating gene-1 KO mice to bone marrow transplantation using IL-2 KO donors, and then followed the reconstitution of the thymus. The thymuses of these mice became increasingly aberrant over time, including abnormalities in both stromal cells and thymocytes. These results demonstrate that IL-2 is critical to several aspects of thymic function, a finding previously obscured by the presence of IL-2 in IL-2 KO mice.
The complement cascade is responsible for more than just the lysis of the foreign cell; the different subcomponents released at each stage are responsible for a number of other biological functions.. Opsonisation. Opsonisation is the process of making a foreign cell more appealing to a phagocytic cell. This is useful as it helps to remove the foreign cell by phagocytosis. The complement component responsible for the opsonisation of cells is C3b. It increases the efficiency of phagocytes as they have specific receptors for the C3b component (C3bR). When the C3b binds to its receptor on the phagocyte, the process of phagocytosis begins and the foreign cell is engulfed.. Anaphylatoxin Formation. The peptide subcomponents C3a and C5a are anaphylatoxins meaning they have a number of inflammation properties. They are able to increase vascular permeability at the site of infection and they are also chemotactic. This means they are able to attract phagocytes into the site of infected tissue.. Both C3a ...
Target cell isolation ideal for chimerism analysis. Positive selection with MACS® MicroBead Technology for multiple cell types from whole blood in 30 min. - 대한민국
Health, ...A pregnant womans blood stream contains not only her own cells but a...Three types of fetal cells have now been identified in the lungs of la...Researchers suspect that fetal cells in a mothers blood stream help h...Using this teams techniques of gene expression analysis researchers ...,3,types,of,fetal,cells,can,migrate,into,maternal,organs,during,pregnancy,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Chimerism genetic testing is used to monitor the success of haemopoietic stem cell transplantation (HSCT) by evaluating the ratio of donor and recipient DNA in the recipients blood or bone marrow.. This guides treatment to help prevent engraftment failure, to maximise graft-versus-tumour effect and to minimise opportunistic infection and graft-versus-host disease.. Flow sorted chimerism analysis of peripheral blood can be used to monitor T-cell, B-cell and myeloid lineage specific engraftment upon request.. ...
MACSprep™ Chimerism CD14 MicroBeads have been developed for the positive selection of CD14+ cells directly from anticoagulated whole blood by using the autoMACS® Pro Separator, the MultiMACS Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis, as well as no washing step after labeling. - USA
As for giving someone cancer in a vaccine. Flat out impossible. Cancer is a malfunction of the DNA in YOUR cells. Because its YOUR cells running amok your bodies immune system sits the fight out.....they dont attack your cells unless the immune system is fubard. Giving you a cancerous cell from someone else wont work. The immune system treats cells from other people lime an infection and kills them swiftly. That is why organ transplants can fail......rejection due to the immune system attacking foreign cells ...
Cells have an internal "clock." This clock tells them how many times they can divide before they must exit the loop by committing cell suicide. This kind of cell death is called apoptosis (pronounced "apohtosis").. Apoptosis is a natural cell process which affects many things. For example, when a human fetus is developing, many vestigial (ancient but no longer necessary) features begin to develop, but mysteriously stop dividing at some point during development. This is another form of apoptosis in action. External factors can also induce a cell to undergo apoptosis. One example is found in our immune system. Certain cells produce factors that are designed to induce apoptosis in the foreign cells they encounter. Other apoptosis inducers include glucocorticoids and radiation. There are many others. Cancer cells grow out of control. They do not know when to stop dividing. They are resistant to the normal factors which induce apoptosis in healthy cells. If you have a normal liver cell and a ...
Husband sperm cell killing wifeAt this time, Rachel in line to see a paper stresses that some humans have a killer cells of foreign cells to invade the body
PepsiCo has come under fire from pro-life advocates because it has been contracting with a research firm that uses fetal cells from babies victimized by
Before receiving a fetal cell transplant several years ago, Peter Sauers Parkinsons was so bad he could barely walk. Today, at 72, he is filled with life and energy.. ...
Paediatric cancer is a disease where the cells of the body grow in an uncontrolled way. Paediatric cancers are those that primarily occur in children. The most common of these are leukaemias, brain tumours and lymphoma. Almost all cancer cells show genetic changes that can be the cause of the cancer or can indicate disease progression.. Paediatric cancer genetic testing is a vital tool, used to identify the cause of the cancer, the type of cancer, help direct the best course of treatment, and to monitor disease progression and the efficacy of treatment.. Chimerism genetic testing is used to monitor the success of blood stem cell transplantation (like bone marrow transplantation). The test looks at the level of donor bone marrow versus the level of bone marrow from the patient (recipient). VCGS has developed an advanced chimerism test which can detect very small changes in the levels of donor and recipient bone marrow. This is used, with high levels of accuracy, to monitor the health of the ...
C3b is the larger of two elements formed by the cleavage of complement component 3, and is considered an important part of the innate immune system. C3b is potent in opsonization: tagging pathogens, immune complexes (antigen-antibody), and apoptotic cells for phagocytosis. Additionally, C3b plays a role in forming a C3 convertase when bound to Factor B (C3bBb complex), or a C5 convertase when bound to C4b and C2b (C4b2b3b complex) or when an additional C3b molecule binds to the C3bBb complex (C3bBb3b complex). C3bs ability to perform these important functions derives from its ability to covalently bind to the surface of invading pathogens within an organisms body. The cleavage of C3 leaves C3b with an exposed thioester bond, allowing C3b to effectively coat and tag foreign cells by covalently binding to hydroxyl (-OH) and amine (-NH2) groups on foreign cell surfaces. This cleavage can occur via three mechanisms (classical pathway, alternative pathway and lectin pathway) that ultimately lead to ...
To the Editor:. The article entitled "Evidence for Cardiomyocyte Repopulation by Extracardiac Progenitors in Transplanted Human Hearts" by Laflamme et al,1 published in Circulation Research, confirms our early observations2 that cardiac chimerism occurs after transplantation of a female heart in a male patient. These results are also in agreement with the detection of chimerism in multiple organs after bone marrow transplantation.3 The authors of the study in Circulation Research are perturbed by the low frequency of chimerism in their samples compared with ours2 (as well as in a subsequent recent report3). They attribute this discrepancy to "…technical differences…" (pages 637 and 638) between the two studies. We agree completely with their assessment.. Given the resolution of the histological sections included in the article in Circulation Research, 1 we are pleasantly surprised that they could identify even this relatively low level of chimerism. Laflamme et al place special emphasis on ...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
Some people possess a small number of cells in their bodies that are not genetically their own; this condition is known as microchimerism. It is difficult to determine potential health effects from this condition because of humans relatively long life-spans. Now, researchers at the University of Missouri have found that microchimerism can be found in dogs as well.
Unit 7: Failures of the Immune System **Even as the human immune system has evolved and developed complex defensive mechanisms against pathogens, pathogens themselves have evolved and developed new means of evading the immune system. In some cases, the adaptations of pathogens are such that they are extremely infectious, virulent, and sometimes even fatal. We are also threatened by health hazards posed by our own immune system. The immune system can, for example, respond inappropriately to its own cells (as in the case of autoimmune diseases) or to innocuous foreign cells (as in the case of allergies and other hypersensitivity diseases). This unit will cover some clinical and medical aspects of the immune system as it relates to immune-system dysfunction. **. Unit 7 Time Advisory ...
Cyclosporin is a major transplant drug and again is an immunosupressant The drug was introduced in 1984 and is used to neutralise the body s own defence system against infections and foreign cells.. As the recipients immune system is affected it can take longer to fight off common infections such as the common cold.. ...
The recent identification of the gammaretrovirus XMRV and a second gammaretrovirus of a different subtype in chronic fatigue syndrome has aroused much interest, not least among sufferers. However, it remains highly controversial whether the detection of these viruses represents true infection or laboratory artifacts.
Anyways, lets get back to the science part(super awesome cool part). From what I understand, this gene PD-1, more or less is in charge of preventing the body from attacking its self. In the case of cancer, the body needs to attack its own cells. This understanding has lead to many treatments including this one, revolving around blocking or getting rid of PD-1. CRISPR is the process of editing out that gene from the T cells, therefore allowing the cells to attack the cancer cells without hesitation. This process reminds me of the p-glo lab we did. Only we inserted a gene into the ecolis DNA ring thing. They focused on the extraction part. This all connects to the concepts we were learning about in genetics. we learned about how enzymes can cut off specific sections of a chromosome based on nucleotide order. That section of DNA could then be introduced into another foreign cells DNA. This having already been done, but mostly to plansts and animals to enhance their production capabilities. Now ...
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...
Coriell Cell Repositories Related Information on MRC-5 Aborted Fetal Cell Line Cell Line Characteristics Repository Number: AG06814 Subcollection:
Obama agency rules Pepsis use of aborted fetal cells in soft drinks constitutes ordinary business operations Saturday, March 17, 2012 by: Ethan A.
If Im going to the trouble of cloning myself, I want the clone to be a copy of me!" Im imagining what someone might say if they were told that their expensive and ethically dubious personal cloning efforts produced a […]. ...
Diverse mammals, including humans, have been found to carry distinct genomes in their cells. What does such genetic chimerism mean for health and disease?. 4 Comments. ...
Diverse mammals, including humans, have been found to carry distinct genomes in their cells. What does such genetic chimerism mean for health and disease?. 4 Comments. ...
Microtransplantation(MST) is an advanced technology to treat malignant hematological diseases and tumors by infusing patients with granulocyte colony-stimulating factor (G-CSF) mobilized human leukocyte antigen (HLA)-mismatched allogeneic peripheral blood stem cells following a reduced-intensity chemotherapy or targeted therapy. The term "microtransplantation" comes from its mechanism of reaching donor cell microchimerism. Chemotherapy is used by lower doses only to destroy cancer and partially suppress patients immune system, which will be reinitiated by donors stem cells soon after transplantation, and will play a role as recipient-versus-tumor (RVT) effect combining donor cells graft-versus-tumor (GVT) effect. Donors stem cells, which have been processed, will also accelerate functional recovery of recipients hematopoietic stem cells, greatly reducing infections and transplant-related mortality. Practices of microtransplantation has shown none graft-versus-host disease (GVHD) till ...
Recipient-derived cells integrate into renal allografts inducing organ-specific microchimerism. Circulating pluripotent progenitor cells with high plasticity for differentiation were suggested as a potential source of allograft chimerism. Whether or not these cells also contribute to tumor formation in renal transplants is unknown. We analyzed six histologically different tumors in renal allografts for the presence of recipient-derived cells. To circumvent dependency on gender mismatch, a polymerase chain reaction assay for highly polymorphic short tandem repeat marker (DNA fingerprinting) in combination with laser microdissection was applied. Pure tumor cell populations were harvested by laser microdissection after immunohistochemical (CD45/CD68) marking of contaminating leukocytes. In cases of gender mismatch (n = 2), results were confirmed by sex chromosome in situ hybridization. Two metanephric adenomas demonstrated microchimerism comprising both donor- and recipient-derived tumor cells. Two ...
Chimerism is defined as the presence in a subject of more than one stable and genetically distinct cell line; cases reported so far include both patients with ambiguous genitalia and healthy subjects. The biological mechanisms, which may give origin to chimeras, are complex, and can be understood by analyzing DNA samples of the patients and their parents using molecular techniques. The objective of this study is to identify the mechanism of origin for the 2 cases we report. The first patient is a phenotipically normal girl with normal (external and internal) genitalia; the second patient had ambiguous genitalia and underwent surgery. DNA was purified from blood samples and, limited to Patient 1, from a sample of biliary cyst. Short tandem repeat polymorphisms were analyzed in order to identify the relative parental contribution to the patients. Molecular analyses carried out on the first patient are not fully informative because of two possible explanations (|i|i.e. |/i|parthenogenetic and andrognetic
BOSTON -- Six organ-transplant recipients have remained healthy for up to five years without anti-rejection drugs, said researchers in the U.S. and Australia whose strategy was chimerism.
From a naturopathic perspective the causes of all autoimmune diseases are the same. Where the autoimmunity manifests is down to genetics and lifestyle. The question is why are autoimmune diseases on the rise? What is it that causes your own immune system to zealously attack itself? Why can the immune system no longer recognise self cells from foreign cells (antigens)?
In experiments in mice, Johns Hopkins Medicine researchers say they have developed a way to successfully transplant certain protective brain cells without the need for lifelong anti-rejection drugs. A report on the research, published Sept. 16 in the journal Brain, details the new approach, which selectively circumvents the immune response against foreign cells, allowing transplanted…
d3] It is important to realize that type-1 diabetes is NOT a conventional allergy to insulin. It is similar to allergies in that it is the bodys immune system overreacting to something that it should not react to, but other than that, is quite different. Allergies involve the immune system overproducing histamines. These histamines attempt to get physical irritants, like pollen, out of your body. You can counter this histamine reaction by taking antihistamines. Type-1 diabetes involves the immune system overproducing malfunctioning killer T-cells (or perhaps under producing regulatory T-cells). These malfunctioning killer T-cells mistakenly kill beta cells, thinking they are foreign cells (ie. living creatures like viruses, that have invaded the body). So the mechanism is different (histamines vs. T-cells), and the mistaken target is different (physical things, like pollen or wheat vs. living organisms, like viruses ...
|b| Symbiotics Lactoferrin with Colostrum Plus |b|Description:|/b| |/b| |ul| |br| Helps Strengthen and Balance Immune Response |br| Helps Enhance Tissue Oxygenation |br| Robs Fuel From Foreign Cells In the Body|/UL| |p|Symbiotics Lactoferrin-Colostrum Pl
In the present condition of the technique of cultivation of tissues, the only possible way of studying leucocytic secretions was to grow colonies of leucocytes in a medium of known properties and to examine the modifications of these properties under the influence of the living cells. The method was far from perfect, because the secretions were mixed with serum and accumulated for 48 hours in a medium where they probably underwent partial destruction. But an approximate idea of certain of the qualities of the secretions, although not of their quantity, could be derived from the experiments. In the fluids extracted from the cultures, we attempted to detect the presence of the leucocytic secretions through their physiological effects on homologous and foreign cells. Two kinds of substances were sought, those which act on homologous cells, and those which destroy foreign erythrocytes.. The secretion by leucocytes of substances necessary to the nutrition of other cells was considered as probable ...
Ariffin, H.; Daud, S.S.; Mohamed, Z.; Ibrahim, K.; Lee, T.F.; Chong, L.A. (2007) Evaluation of two short tandem repeat multiplex systems for post-haematopoietic stem cell transplantation chimerism analysis. Singapore Medical Journal, 48 (4). pp. 333-340. ISSN 0037-5675. ...
Fans know General Hospital loves wacky storylines, and medical chimerism quite easily explains how Charlotte could have DNA of Valentin, Lulu, and Dante but only be the biological child of Lulu and Dante. We know that Claudette was Valentins girlfriend and partner in crime even before she served as the embryo surrogate. Before embryo implantation, fertility treatments can increase odds of success.. With Claudette and Valentin in a sexual relationship, he could have fertilized an egg of Claudettes just as the doctor implanted Lulu and Dantes embryo in her womb. With two embryos, one can be absorbed by the other, resulting in just one baby being born but containing the DNA of the other embryo that didnt survive. In most cases of medical chimerism, it is one twin that absorbs the other, but it could happen with two unrelated embryos in the same uterus.. Valentin and Claudettes embryo could have been absorbed by Dante and Lulus resulting in multiple strains of DNA.. ...
Yes, but isnt it possible that given different selection pressures on human males and females, the autosomal genes of males and females will be found to differ in important respects? Given the very important differences in female and male development and physiology, arent autosomal differences likely to be very important too? How will we know unless we sample a large number of males and a large numbers of females? And if, as Leiden reports, this is the first time the DNA of a human female has been fully sequenced (whereas half a dozen or so males have been sequenced), isnt that a legitimate thing to do scientifically, regardless of the way in which the completion of the sequencing is announced? Doesnt if reflect a inappropriate (sex-linked) bias to sample only the DNA sequences of males ...
The point of the article is not that there is specifically male DNA that is within the female DNA of the mothers brain. Rather, the point of the article is to show that a fetus DNA can be found in a mothers brain. Because this DNA comes from a different individual (not because the DNA comes from a male), this DNA may act differently than the mothers own DNA (although its not yet clear from the research if the DNA is doing anything ...
Average salaries for FMC Technologies Rotation Engineer: $5,857. FMC Technologies salary trends based on salaries posted anonymously by FMC Technologies employees.
A thorough description on how to perform immunostaining directly on CytoChipNano after CTC capture is performed by CytoQuest™ CR. Abnovas CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information. - CytoQuest™ CR CTC Immunostaining - AbVideo™ - Support - Abnova
Caltag Medsystems supply devices to isolate cicrulating tumor cells, cicrculating foetal cells and Circulating Tumour Microemboli
Women with RA ask why its so common to acquire severe Rheumatoid Arthritis after pregnancy the way I did. I dont blame my son since my symptoms went back
Stem cells have huge potential in medicine because they have the ability to differentiate into many different cell types potentially providing doctors with the ability to produce cells that have been permanently lost by a patient. For instance, the damage of neurodegenerative diseases like Parkinsons, in which dopaminergic neurons in the brain are lost, may be ameliorated by regenerating neurons. Another example of a potential medical application is Type 1 diabetes, an autoimmune condition in which pancreatic islet cells are destroyed by the bodys immune system. Because stem cells have the power to differentiate into islet cells, stem cell therapy could potentially cure this chronic condition. However bright this promise, many barriers must be overcome before stem cells can be used in medicine. Stem cell therapy would be most effective if you could use your own stem cells, since using ones own cells would avoid potential complications from immune rejection of foreign cells. However, in ...
Researchers at the University of Adelaide in South Australia have assembled strong evidence to suggest the bodys immune system can develop an inflammatory response that kills brain cells. Research Assistant Danielle Fornarino said millions of people were affected by neurodegenerative diseases and dementiaDementia but there had previously been no definitive cause. People had looked at various forms of proteins that can be produced in neurodegenerative diseases, which can have a tendency to aggregate, she said. Thats definitely the case with Alzheimers disease and there have been longstanding studies that have looked at the potential involvement of protein deposits called amyloid plaques. We have a model for Huntingtons that looks at the type of cellular signalling that occurs and through our genetic interactions studies we identified the innate immune system as being directly involved in cellular toxicity. The immune system is the bodys defence against disease and foreign cells but is ...
A type of immune cell that can kill certain cells, including foreign cells, cancer cells, and cells infected with a virus. Cytotoxic T lymphocytes can be separated from other blood cells, grown in the laboratory, and then given to a patient to kill cancer cells. A cytotoxic T lymphocyte is a type of white blood cell and a type of lymphocyte. Also called cytotoxic T cell and killer T cell ...
Transplantation has become standard of care to treat end-organ failure, replacing a failed organ with a functioning one. However, the toxicity of the immunosuppressive ag..
Researchers from the Salk Institute of Biological Studies were able to combine pig cells and human cells to create chimeric cells. The edited cells are helping scientists understand early embryonic ...
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Dow Chemical (NYSE: DWDP) and FMC (NYSE:FMC) are both large-cap basic materials companies, but which is the superior investment? We will contrast the two businesses based on the strength of their institutional ownership, risk, dividends, analyst recommendations, profitability, earnings and valuation. Valuation & Earnings This table compares Dow Chemical and FMCs top-line revenue, earnings per […]
The term chimerism in the setting of hematopoietic stem cell transplantation refers to the engraftment or presence of lymphohematopoietic cells of donor origin, typically detected by using techniques of DNA analysis (18). Mixed chimerism, in contrast to full donor chimerism, indicates the presence of both donor and recipient lymphohematopoietic cells. Because mixed versus total donor chimerism can now be reliably generated with specific NST regimens and avoided with other NST regimens, investigators must address an important question: is the state of mixed chimerism after transplantation desirable?. There are at least 3 theoretical advantages of mixed chimerism over full chimerism as an approach to transplantation tolerance (19), as follows: 1) mixed chimerism can be achieved with nonmyeloablative regimens that are less toxic, 2) mixed chimeras have better immune function, and 3) thymic deletion of host-reactive cells occurs to a greater degree in mixed chimeras due to the intrathymic presence ...
Read "Pulmonary endothelial chimerism after hematopoietic stem cell transplantation, Surgery Today" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Successful induction of allograft tolerance has been achieved in nonhuman primates (NHPs) and humans via induction of transient hematopoietic chimerism. Since allograft tolerance was achieved in these recipients without durable chimerism, peripheral mechanisms are postulated to play a major role. Here, we report our studies of T cell immunity in NHP recipients that achieved long-term tolerance versus those that rejected the allograft (AR). All kidney, heart, and lung transplant recipients underwent simultaneous or delayed donor bone marrow transplantation (DBMT) following conditioning with a nonmyeloablative regimen. After DBMT, mixed lymphocyte culture with CFSE consistently revealed donor-specific loss of CD8+ T cell responses in tolerant (TOL) recipients, while marked CD4+ T cell proliferation in response to donor antigens was found to persist. Interestingly, a significant proportion of the proliferated CD4+ cells were FOXP3+ in TOL recipients, but not in AR or naive NHPs. In TOL recipients, ...
B10.A(5R) mice (H-2i5), immunized with spleen cells from congenic B10 mice (H-2b), responded to alloantigens of the H-2Db region by producing antibodies of only IgM type. In contrast, they produced both IgM and IgG antibodies when immunized with noncongenic H-2b cells that carry other foreign cell surface antigens (non-H-2) in addition to H-2Db. A hypothesis was proposed comparing the H-2Db antigen on a congenic cell to a hapten on a nonimmunogenic carrier which fails to induce T-cell helper function responsible for the switch from IgM to IgG secretion in B cells. Data presented here confirmed this hypothesis. 5R mice rendered tolerant to the relevant non-H-2 antigens were unable to mount the anti-H-2Db IgG response in a noncongenic immunization. Tolerance induction did not lead to abrogation of the T-cell mediated cytotoxicity. ...
As part of the study, the researchers tried to boost insulin production in the macaques by performing a second transplant. Four weeks after transplanting the embryonic pig cells, they implanted adult insulin-producing pig islet cells into the kidneys of the macaques. The embryonic cells primed the monkeys immune system to accept the mature islet cells, without using anti-rejection drugs, but their glucose control did not continue to improve.. Hammerman. Hammerman suspects this may be because they did not transplant enough adult pig islet cells or that placing them in the kidney is not an optimal location. As a comparison, the researchers also transplanted adult pig islet cells into a control group of diabetic macaques that did not receive the earlier embryonic pig cell transplants. Not surprisingly, the macaques immune systems attacked the foreign cells and eventually rejected those cells all together.. The new study builds on earlier research in rats, reported by the same group last year in ...
Antibody. Computer graphic of a molecule of immunoglobulin G1, a type of antibody protein that the immune system uses to fight infections. Like all proteins, it is comprised of amino acid groups. Immunoglobulin G is the most common antibody found in blood. They are produced by lymphocytes (a type of white blood cell) in response to the proteins (antigens) found on foreign cells such as bacteria and viruses. Each antibody recognises a specific antigen, either destroying or neutralising it. Immunoglobulin G can cross a mothers placenta, making it important in the immune response of a developing foetus. - Stock Image P270/0045
A proposed solution to this problem is the idea of "Encapsulated Cell Therapy" (Galletti, 1995). It basically works as follows: The cells used can be either human or other animal cells. These cells are genetically altered to produce whatever it is that is needed in the body (insulin, other needed proteins, hormones, etc.). The cells are then protected from the immune system in a very clever "capsule" system made of a polymer material. This polymer has specially designed pores that are large enough to allow the desired excretion of therapeutic substances, but small enough to keep out the bodys immune system. In effect, we have a cybernetic system here, in which an isolated "colony" of foreign cells lives, protected, inside the humans system. This colony feeds off of the humans blood, and excretes its wastes and its specially designed products into the humans circulatory system.. There are other applications for this type of technology such as pain control systems and treatment of otherwise ...
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Doctors give trusted, helpful answers on causes, diagnosis, symptoms, treatment, and more: Dr. Higginbotham on paternity testing pregnant: Non invasive paternity testing is a technology based on detection of fetal cells in the mothers blood. A variety of specialty labs offer this service and cite the ability to find such results as early as 8 weeks into the pregnancy. Traditional testing requires fetal cells from invasive procedures like sampling amniotic fluid by needle or the placental cells.
iframe src="https://biblio.ugent.be/publication?limit=100&q=parent+exact+%22CHIMERISM%22&embed=1&hide_pagination=1&hide_info=1&hide_options=1&hide_cluster=1" ...
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BOSTON and BELLSHILL, Scotland, Aug. 31, 2011 /PRNewswire/ -- Texas FMC Technology Expands Presence in Scotland. Global energy firm increases...
TY - JOUR. T1 - Mixed Chimerism and Secondary Graft Failure in Allogeneic Hematopoietic Stem Cell Transplantation for Aplastic Anemia. AU - Adult Aplastic Anemia Working Group of the Japanese Society for Hematopoietic Cell Transplantation. AU - Kako, Shinichi. AU - Yamazaki, Hirohito. AU - Ohashi, Kazuteru. AU - Ozawa, Yukiyasu. AU - Ota, Shuichi. AU - Kanda, Yoshinobu. AU - Maeda, Tetsuo. AU - Kato, Jun. AU - Ishiyama, Ken. AU - Matsuoka, Ken ichi. AU - Miyamoto, Toshihiro. AU - Iida, Hiroatsu. AU - Ikegame, Kazuhiro. AU - Fukuda, Takahiro. AU - Ichinohe, Tatsuo. AU - Atsuta, Yoshiko. AU - Mori, Takehiko. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Mixed chimerism (MC) and/or secondary graft failure (SGF) with recipient- or donor-type chimerism is a major obstacle in allogeneic transplantation for aplastic anemia (AA). From a registry database in Japan, patients with AA age ,15 years who underwent a first allogeneic bone marrow or peripheral blood stem cell transplantation between 2000 and 2014 and ...
We analyzed the clinical course and risk factors of 18 patients with poor engraftment after allogeneic bone marrow transplantation (BMT), defined as absolute neutrophil count below 0.1×10 9/1 28 days post-BMT. Significant risks associated with non-engraftment included HLA one antigen mismatch, BMT from matched unrelated donor, and a low dose of colony-forming units-granulocyte-macrophage (,10 4/kg). Examined by a semi-quantitative analysis of polymorphic microsatellite markers, donor DNA chimerism on day 28 was found to be predictive of treatment outcome. Seven patients had detectable donor DNA, varying from 43 to 100%. Five of them responded to granulocyte colony-stimulating factor (G-CSF) and achieved engraftment. Two were given further infusions of peripheral blood hematopoietic stem cells (PBSC) from the same donors, resulting in engraftment in one of them. Eleven patients had no detectable donor DNA, and none responded to G-CSF. Autologous regeneration occurred in six of these patients, ...
Our clinical investigations demonstrate, that nonmyeloablative stem cell transplantation (NST) is a novel therapeutic option in patients with high-risk ALL or AML. NST can be administered to patients eligible for allogeneic stem cell transplantation with contraindications against high-dose radio- and chemotherapy (standard SCT). After NST, infections and late onset acute graft-versus-host disease (GvHD) frequently occurred, but transplant-related mortality was low in contrast to standard SCT. Leukemia-free survival and overall survival were similar after NST and standard SCT. A higher relapse rate after NST was balanced by a higher transplant-related mortality after standard SCT. The conditioning regimen itself had no relevant impact on survival. Sequential chimerism analyses of leukocyte subpopulations resulted in early diagnosis of mixed chimerism, which proved to be predictive for later relapses. Stable mixed chimerism was not established in these patients. Adoptive immunotherapy in patients, ...
Type 1 diabetes, the problem that concerns Melton, is well-suited to stem-cell therapy. It involves a single cell type, the beta cell, that is either missing or present in numbers too low to regulate blood-sugar levels. "If you could place that cell type back into a person [so] that it was not subjected to autoimmune attack, where it could be healthy and thrive, even outside the context of the pancreas, then you could cure diabetes." One current therapy for type 1 diabetes - the Edmonton protocol-involves injecting beta cells from three cadavers into a patients vein; the cells seed the liver and work from within that organ. But nonsteroidal immunosuppressants are required to control both autoimmune attacks and rejection of the foreign cells by the patients body. Nevertheless, a first test of beta cells created through directed differentiation of human [embryonic stem] cells might be to use them in the Edmonton protocol. The next step might be to create a device-beta-cell tissue grown on a ...
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If you plan to have a Pap test, make your appointment for a time when you are not bleeding. Dont put anything in the vagina for 3-4 days before your appointment - no douches, tampons, medications, spermicides, diaphragms, fingers, intercourse, or oral sex. Any foreign cells or outside products can interfere with the Pap test. If you are having a problem, try not to schedule your appointment after you have used any vaginal medications or soon after having sex. Vaginal medications make it impossible to visualize the discharge or vaginal tissue. Intercourse acts like a douche and makes the discharge difficult to see or collect for testing. ...
Rheumatoid nodules are lumps that form in most patients with rheumatoid arthritis. Read more about the causes, complications, and treatments.
Can you imagine if animals were used instead of human babies? How many products do we purchase which are clearly marked with those reassuring words: No animals were used in the testing of this product. What has become of us? The world has gone mad. What else are we eating, drinking, using as cosmetics, etc. which…
The team identified donor-cell tissue infiltration or extensive peripheral blood donor-cell chimerism in 7 patients.. The doctors determined that the majority of cases were resolved with steroid administration and optimization of tacrolimus immunosuppression.. Dr George Mazariegos and colleagues concluded, "The incidence of histologically proven graft versus host disease after clinical intestinal transplantation is 6.5% (8/122) in children and 4.7% (6/128) in adults". "Successful clinical management requires a high index of suspicion to minimize morbidity and mortality". "Diagnostic and treatment strategies based on this experience are proposed". ...
Genetically distinct fetal cells can remain in a mothers body for decades after birth and can even incorporate themselves into her organs. (via: trendolizer.com)
Creative Biolabs provides Lenti-CD22 CAR (scFv-CD3ζ, FMC63) Viral Particle product for Biopharmaceutical research,preclinical and clinical trials.
Conzelmann M and Jékely G. Antibodies against conserved amidated neuropeptide epitopes enrich the comparative neurobiology toolbox. EvoDevo, 2012, 3:23. Conzelmann M, Offenburger SL, Asadulina S, Keller T, Münch TA, Jékely G. Neuropeptides regulate swimming depth of Platynereis larvae. Proc Natl Acad Sci U S A. 2011. 108(46): p. E1174-83. Jékely G. Origin and early evolution of neural circuits for the control of ciliary locomotion. Proc Biol Sci. 2011. 278(1707): p. 914-22. Jékely G. Evolution of phototaxis. Philos Trans R Soc Lond B Biol Sci. 2009. 364(1531):2795-808. Satir P, Mitchell DR, and Jékely G. How did the cilium evolve? Curr Top Dev Biol. 2008. 85: p. 63-82. Jékely G, Colombelli J, Hausen H, Guy K, Stelzer E, Nédélec F, Arendt D. Mechanism of phototaxis in marine zooplankton. Nature, 2008. 456(7220): p. 395-9. Jékely G. Origin of the nucleus and Ran-dependent transport to safeguard ribosome biogenesis in a chimeric cell. Biol Direct, 2008. 3: p. 31. Jékely G. Evolution of ...
Obviously, this be a shock to the parent (but to no one else) if the parent were the father. It happens. Not exactly a medical mystery what may have happened.. But in this case, the parent was the mother. Who had become pregnant, carried all her children to to term, and then gave birth to them naturally. No implanted embryos or in vitro fertilizatin or other such medical intervention was involved. Karen Keegan developed kidney problems and needed a transplant. Naturally, they checked her children for a genetic match. They found something that shocked her: her childrens DNA did not match hers. She was not not their natural mother.. It turns out the woman was a "chimera;" she had two separate DNA profiles. One in her blood, another in her reproductive system. Chimerism occurs in the embryo as one is devoloping -- two fraternal twins embryos grow, but then one absorbs the other, incorporating the others DNA into different tissues and organs of its body. Most of her body had one set of DNA, but ...
Eventbrite - FMC/PLC Education Committee presents FMC/PLC Fall Update 2015 - Attention to Detail - Wednesday, 21 October 2015 at Cardel Theatre, Calgary, AB. Find event and ticket information.
Researchers from the Sound Choice Pharmaceutical Institute recently published a study showing a correlation with the introduction of human fetal cell lines used
Creative Biolabs provides Lenti-CD22 CAR (scFv-41BB-CD3ζ, FMC63) Viral Particle product for Biopharmaceutical research,preclinical and clinical trials.
Investigations into four serious breaches of vessel fishing licenses have begun after a surveillance sweep in the Pacific tuna fishery.
Sixteen Lewis (RT1l) rats were exposed to sublethal dose (7Gy) of Cs-137 TBI. Irradiated rats were divided into 4 experimental groups (n=4 each) based on therapeutic approach: Group 1 - no intervention; Group 2 - normal saline injection; Group 3 - allogeneic bone marrow transplantation (BMT) [ACI (RT1a)]; and Group 4 - DRCC transplantation [ACI/LEW (RT1a/RT1l]. Saline as well as cellular therapeutics were delivered into the femoral bone at 6 hours following TBI. DRCC were created by ex-vivo fusion of bone marrow cells derived from fully MHC mismatched LEW (RT1l) and ACI (RT1a) donors. Briefly, donor and recipient cells were isolated, separately stained with the PKH26 and PKH67 fluorescent dyes, fused with polyethylene glycol (PEG) and sorted by flow cytometry. Cells presenting double staining were transplanted in a dose 10-12 x 106. All animals received supportive treatment with prophylactic antibiotics, fluids, and softened food during 90-days follow-up. Blood samples were evaluated using ...
CHRU Nancy - Centre Hospitalier Régional Universitaire de Nancy (Siège social : 29 avenue du Maréchal de Lattre de Tassigny, CO 60034, 54035 NANCY CEDEX Hôpital Central : 29 Avenue du Maréchal de Lattre de Tassigny, 54035 Nancy Hôpitaux de Brabois / Enfants : Rue du Morvan, 54500 Vandœuvre-lès-Nancy Maternité régionale :10 Avenue Dr Heydenreich, 54000 Nancy - France) ...
Rheumatoid arthritis, or RA, is a form of inflammatory arthritis and an autoimmune disease. For reasons no one fully understands, in rheumatoid arthritis, the immune system - which is designed to protect our health by attacking foreign cells such as viruses and bacteria - instead attacks the bodys own tissues, specifically the synovium, a thin membrane that lines the joints.
While rheumatoid nodules are the most common extra-articular manifestation of rheumatoid arthritis, in this patient they were found in a very unusual location.
Cell, Cells, Donor, Graft, Mice, Donors, Spleen, Transplantation, Cell Proliferation, Survival, T-cell, Transplant, Tumor, Bone, Bone Marrow, Cell Transplantation, Chimerism, Disease, Graft-versus-host Disease, Hematopoietic Stem Cell
TY - JOUR. T1 - Analysis of cosegregation of intragenic DNA sequence variations as markers of maternal cell contamination in prenatal diagnosis of β-thalassemia. AU - Saadi, Abdul V.. AU - Girisha, Katta M.. AU - Gopinath, Puthiya M.. AU - Satyamoorthy, Kapaettu. PY - 2011/3. Y1 - 2011/3. N2 - Prenatal diagnosis of 3 HBB gene mutations causing β-thalassemia and hemoglobin D Punjab segregated in a South Indian nuclear family is reported along with a method identified as control for maternal cell contamination (MCC). Amplicons of the HBB gene from genomic DNA obtained from the blood of a thalassemic first child (proband), both parents, and a chorionic villus sample of their second pregnancy were directly sequenced. A test for MCC was performed by genotyping polymorphic microsatellite markers (D21S11 and D21S1270) by quantitative fluorescence polymerase chain reaction (QF-PCR) and capillary gel electrophoresis. The pedigree analysis showed proband as a compound heterozygote of ...
Following a bone marrow transplant, patients are monitored closely for evidence of graft rejection or recurrence of the original disease. Bone marrow transplantation creates a donor-recipient cellular chimerism in the patient, which can be quantitively measured through short tandem repeat (STR) analysis of peripheral whole blood to determine the percent chimerism of the sample. Increasing recipient chimerism is an indication of graft rejection or relapse. Software programs designed to analyze forensic mixture samples have the potential to be useful in analyzing post-transplant mixed chimeric samples. Post-transplant samples were analyzed using three mixture deconvolution software programs. The programs were fast, accurate and consistent in determining the mixing proportions of the samples and the three programs gave concordant results.
TY - JOUR. T1 - Kartagener-syndromás no kiviselt ikerterhessége. AU - Gávai, Márta. AU - Beke, A.. AU - Urbancsek, J.. AU - Murber, Ákos. PY - 2006. Y1 - 2006. N2 - In females, the immobility of the cilia in the fallopian tube may cause barrenness. Pregnancy does not affect the frequency and severity of existing bronchitis, bronchiectasia and bronchial asthma caused by primary ciliary dyskinesia. There is an increased risk of premature delivery in patients with Kartagener syndrome. Since assisted reproduction is often used, the risk of multiple gestations is higher. Along with multiple gestation comes the increased risk of premature delivery, not only because of the presence of multiple fetuses and uterine stretching, but also because the increased size of the uterus presses against the already poorly functioning maternal lungs and can lead to infection and early rupture of the membranes. In cases where a cesarean section becomes necessary, it is very important to use the appropriate ...
Epigenetic alterations are increasingly recognized as causes of human cancers and disease. These aberrations are likely to arise during genomic reprogramming in mammalian preimplantation embryos, when their epigenomes are most vulnerable. However, this process is only partially understood because of the experimental inaccessibility of early-stage embryos. Here, we introduce a methodologic advance, probing single cells for various DNA-methylation errors at multiple loci, to reveal failed maintenance of epigenetic mark results in chimeric mice, which display unpredictable phenotypes leading to developmental arrest. Yet we show that mouse pronuclear transfer can be used to ameliorate such reprogramming defects. This study not only details the epigenetic reprogramming dynamics in early mammalian embryos but also suggests diagnostic and potential future therapeutic applications. Science 2013 Sep 6; 341(6150):1110-2.
Of course, if a persons life depends on receiving an oral medicine that contains non-kosher ingredients, he is certainly allowed to have it. Even if there is no danger to a persons life, and a non-kosher medicine is prescribed, there are a few instances where its usage would be allowed. For instance, if the medicine has no nutritional value and is not considered fit for human consumption, nor for that of a dog [which is the criteria for determining of its in the category of "food"], then it is allowed. Medicines in capsules or medicines which have a bitter taste are examples. Even if an otherwise bitter medicine is coated with sugar, this does not transform it into food and most halachic authorities consider these medicines unfit for a dog. Also, if the forbidden ingredients were altered chemically, or if they have completely dried out, then the medicine is permitted, even if the patients life in not in danger[5 ...
In contrast, PDP-FISH has been reported for three CNV loci using fosmid probes, which distinguish signal presence and absence rather than signal intensity differences pod-FISH has already been successfully used to identify the parental origin of individual derivative chromosomes, such as the characterization of chimerism and uniparental disomy 15. PLoS ONE Alerts: New Articles. ...
Results: In untreated control groups (n=5) animals rejected skin grafts, solid organ grafts and hind limb transplants acutely by POD 14 (± 1 day), POD 9 (± 2 days), and 8 (± 1 day), respectively. The induction regimen extended skin graft survival to 32 ± 8 days (n=5). Additional donor BM augmentation lead to allograft survival of 150 days in 4 of 5 recipients. However, indefinite graft survival of ,150 days was observed in all animals receiving the induction regimen and a VCA. Mixed chimerism analysis showed engraftment of donor BM in groups receiving BM at the time of skin transplantation (6.8% ± 3.1%). In groups receiving a VCA alone or an allograft augmented with donor BM and splenocytes, donor chimerism was detected at 22.51% ± 5.96% and 30.17% ± 8.72%, respectively. Vβ-T cell receptor staining showed decreased expression of Vβ 5.1/5.1 and Vβ 8.1/8.2 in animals receiving the full induction regimen compared to controls indicating a central selection and depletion mechanism for ...
Lymphocyte reconstitution following non-myeloablative hematopoietic stem cell transplantation follows two patterns depending on age and donor/recipient chimerism.
In this study, the puzzling phenomenon of spontaneous coexistence of D-positive and D-negative RBC subpopulations was investigated. In a representative number of 9 patients with mixed Rh phenotype, the prevailing molecular background of this condition was elucidated. Rh mosaicism involving a hematopoietic stem-cell line with LOH on chromosome 1 was demonstrated to be the key mechanism for the observed RBC phenotype anomalies.. Frequent causes of mixed Rh phenotype, such as RBC transfusion or HSCT, had been ruled out in these patients, which was also confirmed by the fact that Rh and in one case also Fy but not other blood group antigens were affected. In addition, none of the patients displayed congenital or acquired chimerism,4 as evidenced by microsatellite analysis. This result was remarkable, as spontaneous chimerism had been reported to occur quite frequently.5,46. The immunohematologic properties of the D-positive and D-negative RBC subpopulations indicated loss of one complete RH ...
Tsvetelin Lukanov has earned his Bachelor and Master of Science degree at Sofia University in the field of Molecular Biology and Gene Engineering. He has a Doctor degree and Laboratory Immunology Residency at Medical University, Sofia Bulgaria. The focus of his doctorate was on immunogenetic markers - HLA and non-HLA, that influence the HSCT outcome, as well as on the chimerism and its role as a predictive marker in post-SCT monitoring. Currently, Dr. Lukanov works at the Department of Clinical Immunology and Stem Cell Bank, University Hospital Alexandrovska, Sofia. His professional focus is on chimerism, immunogenetics, solid organ and stem cell transplantations and etc.
Natalie is feeling much better already. We are so glad she is here, had we not come in it could have been very bad. The bacteria she developed is something that most people just have on their skin and isnt washed off in the shower or bath. The doctors suggested that the bacteria just found its way into her body, possibly from her central line. They also said it is more amazing that she has not had this sooner, than it is that she has it at all. This hospital stay feels like a walk in the park, no biggie. A couple days is just a little wrinkle in our way. We learned today that Alices chimerism test came back and now shows 95% donor cells. She is getting closer to the goal of 100% donor. Both Natalie and Alices neutrophil antibodies no longer appear in their blood. This is also great news. Another test will be taken regarding Natalies Tcells. The past test showed more host Tcells than they anticipated, however, they feel it wasnt accurate. More results next week. Tomorrow is day 99 for ...
Noninherited maternal antigens identify acceptable HLA mismatches: Benefit to patients and cost-effectiveness for cord blood banks. Biology of Blood and Marrow Transplantation. 2014 ...
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Goat Freemartin TestingGoat Freemartin Testing

A DNA test that detects presence of Y-chromosome is available to confirm chimerism in the blood of females suspect of being ...
more infohttp://www.vgl.ucdavis.edu/services/freemartin-goat.php

Chimerism - A Clinical Guide | Nicole L. Draper | SpringerChimerism - A Clinical Guide | Nicole L. Draper | Springer

... state-of-the-art guides focused on the clinical aspects and consequences of chimerism ... Chimerism. Book Subtitle. A Clinical Guide. Editors. * Nicole L. Draper Copyright. 2018. Publisher. Springer International ... Written by experts in the field, Chimerism: A Clinical Guide is a valuable resource for clinicians and researchers that will ... This text provides a comprehensive, up-to-date review of chimerism. The first part of the volume presents the causes of ...
more infohttps://www.springer.com/us/book/9783319898650?wt_mc=

RE: [Histonet] ChimerismRE: [Histonet] Chimerism

... From:. Tony Henwood I believe this is known as Proteus syndrome. The following is from a paper I am ... Chimerism Hi Kemlo, >The only times I have come across chimerism has been associated with >blood transfusion. The patient ... and has chosen Chimerism (Genetic Mosaicism) about which >I know nowt. Does anyone out there know anything about this subject ...
more infohttp://www.histosearch.com/histonet/Apr06/RE.HistonetChimerismC.html

Kidney and Liver Transplant Rejection Purged by Chimerism | Medpage TodayKidney and Liver Transplant Rejection Purged by Chimerism | Medpage Today

... and Australia whose strategy was chimerism. ... Kidney and Liver Transplant Rejection Purged by Chimerism. ... Sachs said this period of transient chimerism reflects a normal selection process in the thymus by which immune cells that ... But the new results show how chimerism and tolerance might be applied to a broad population of transplant recipients. ... Both U.S. groups sought to induce chimerism by infusing donor hematopoietic cells along with the organ transplant. ...
more infohttps://www.medpagetoday.com/surgery/transplantation/8085

Chimerism, DonorChimerism, Donor

... , Interpretation. Resultable. N. For questions regarding the Interface Map, please contact [email protected] ... Chimerism, Donor, Informative Loci. * Component test codes cannot be used to order tests. The information provided here is not ... See CPT codes under (Chimerism, Recipient Pre-Transplant - test code 2002065) Are you an ARUP Client?. Click here for your ... Background Information for Chimerism, Donor:. Indication: Monitoring for bone marrow transplant patients; correlation with ...
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Rheumatoid Arthritis:Tolerance Induction by Mixed Chimerism - Study Results - ClinicalTrials.govRheumatoid Arthritis:Tolerance Induction by Mixed Chimerism - Study Results - ClinicalTrials.gov

Rheumatoid Arthritis:Tolerance Induction by Mixed Chimerism. The safety and scientific validity of this study is the ... One patient was in complete remission, RF neg, no GVHD, stable mixed chimerism, died 4 years after treatment from leukemia ...
more infohttps://www.clinicaltrials.gov/ct2/show/results?term=stem+cell+arthritis+AND+Arthritis&rank=16&view=results

Rheumatoid Arthritis:Tolerance Induction by Mixed Chimerism - Full Text View - ClinicalTrials.govRheumatoid Arthritis:Tolerance Induction by Mixed Chimerism - Full Text View - ClinicalTrials.gov

Rheumatoid Arthritis:Tolerance Induction by Mixed Chimerism. The safety and scientific validity of this study is the ...
more infohttps://clinicaltrials.gov/ct2/show/NCT00282412

Chimerism and Tolerance in Solid Organ Transplantation | OMICS InternationalChimerism and Tolerance in Solid Organ Transplantation | OMICS International

The chimerism was comprised of myeloid (neutrophil) chimerism with little or no T cell chimerism. A donorspecific renal ... Large Animal Chimerism and Tolerance Studies. The translation of mixed BM chimerism-induced tolerance from studies in mice has ... Fifteen of them developed multilineage chimerism without the occurrence of GVHD. In 8 patients with long term chimerism (,6 ... Investigators at Emory recently described compartmentalized chimerism or split chimerism [32]. A high-level of whole blood ...
more infohttps://www.omicsonline.org/chimerism-and-tolerance-in-solid-organ-transplantation-2155-9899.S9-003.php?aid=9316

EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection KitEasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit

HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells with an EasySep™ magnet from freshly ... EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit (Catalog #17875). *EasySep™ HLA Chimerism Buffy Coat CD56 ... The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared ... RoboSep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit with Filter Tips (Catalog #17875RF) ...
more infohttps://www.stemcell.com/products/product-types/cell-isolation-products/easysep-hla-chimerism-buffy-coat-cd56-positive-selection-kit.html

Molecular Diagnostics Lab - Chimerism Assessment by Polymorphic
Microsatellite Markers | MD Anderson Cancer CenterMolecular Diagnostics Lab - Chimerism Assessment by Polymorphic Microsatellite Markers | MD Anderson Cancer Center

Chimerism testing for assessment of stem cell engraftment is performed on genomic DNA extracted from peripheral blood or bone ... Chimerism Assessment by Polymorphic Microsatellite Markers (Post-Transplant), PCR. Pre-transplant Screening ... This unique marker will be used for subsequent Chimerism analysis by importing pre & donors profiles from archives for analysis ...
more infohttps://www.mdanderson.org/research/research-resources/core-facilities/molecular-diagnostics-lab/services/chimerism-assessment-by-polymorphic-microsatellite-markers.html

JCI -
Endothelial chimerism and vascular sequestration protect pancreatic islet grafts from antibody-mediated rejectionJCI - Endothelial chimerism and vascular sequestration protect pancreatic islet grafts from antibody-mediated rejection

Endothelial chimerism and vascular sequestration protect pancreatic islet grafts from antibody-mediated rejection. ... Endothelial chimerism and vascular sequestration protect pancreatic islet grafts from antibody-mediated rejection. ... Thus, we conclude that endothelial chimerism combined with vascular sequestration of DSAs protects islet grafts from humoral ...
more infohttps://www.jci.org/articles/view/93542/figure/1

Lineage-specific chimerism analysis | MACS Cell Separation   - FranceLineage-specific chimerism analysis | MACS Cell Separation - France

Target cell isolation ideal for chimerism analysis. Positive selection with MACS® MicroBead Technology for multiple cell types ... MACSprep™ Chimerism MicroBeads allow the fast and easy isolation of target cells for chimerism analysis. Get B cells, NK cells ... Cell isolation for lineage-specific chimerism analysis. *Direct cell isolation for reliable lineage-specific chimerism analysis ... MACSprep™ Chimerism MicroBeads have been developed for the positive selection of cells directly from anticoagulated whole blood ...
more infohttps://www.miltenyibiotec.com/FR-en/products/macs-cell-separation/cell-separation-reagents/macsprep/macsprep-chimerism-microbeads.html

Lineage-specific chimerism analysis | MACS Cell Separation   - EspañaLineage-specific chimerism analysis | MACS Cell Separation - España

Target cell isolation ideal for chimerism analysis. Positive selection with MACS® MicroBead Technology for multiple cell types ... MACSprep™ Chimerism MicroBeads allow the fast and easy isolation of target cells for chimerism analysis. Get B cells, NK cells ... Cell isolation for lineage-specific chimerism analysis. *Direct cell isolation for reliable lineage-specific chimerism analysis ... MACSprep™ Chimerism MicroBeads have been developed for the positive selection of cells directly from anticoagulated whole blood ...
more infohttps://www.miltenyibiotec.com/ES-en/products/macs-cell-separation/cell-separation-reagents/macsprep/macsprep-chimerism-microbeads.html

human chimerism Archives - The Nichehuman chimerism Archives - The Niche

human chimerism. Surprising reason why human cloning may produce someone else July 5, 2018 Paul Knoepfler 4 ...
more infohttps://ipscell.com/tag/human-chimerism/

Tandem chimerism - WikipediaTandem chimerism - Wikipedia

Parra et al., Tandem Chimerism as a means to increase protein complexity in the human genome Parra et al., Tandem chimerism as ... Tandem chimerism is the phenomenon where two consecutive genes are transcribed into a single RNA molecule. The translation ( ...
more infohttps://en.wikipedia.org/wiki/Tandem_chimerism

Mixed Chimerism Prolong Mice Keratoplasty Allografts Survival | IOVS | ARVO JournalsMixed Chimerism Prolong Mice Keratoplasty Allografts Survival | IOVS | ARVO Journals

Mixed Chimerism Prolong Mice Keratoplasty Allografts Survival You will receive an email whenever this article is corrected, ... Results: : While pretreatment with SEB induced high levels of hematopoietic chimerism on day 14(4.89±0.71%) and(3.19±0.52%)on ... While pretreatment with CTX resulted in chimerism(3.10±0.20%), which decreased by day 28 (1.42±0.31%). Pretreatment with NS ... Purpose: : To explore the mixed chimerism and specific immune tolerance induced by superantigen SEB combined with the donor ...
more infohttp://iovs.arvojournals.org/article.aspx?articleid=2350977

Post-Transplant Chimerism Analysis | Center for Human Genetics | University Hospitals Cleveland Medical Center | Cleveland, OHPost-Transplant Chimerism Analysis | Center for Human Genetics | University Hospitals Cleveland Medical Center | Cleveland, OH

Chimerism analysis is one part of the monitoring process for bone marrow transplant patients. The pre-transplant chimerism ... Post-transplant chimerism complex: $839.30 / 81267. Please submit a completed Hematology/Oncology Specimens requisition for ... The number of alleles in the profile established by the STR panel at the time of the pre-transplant chimerism analysis will ... determine the complexity of the post-transplant chimerism analysis. Screen-informative STRs from post-transplant recipient ...
more infohttp://www.uhhospitals.org/cleveland/services/genetics/laboratory/tests-offered/post-transplant-chimerism-analysis

Fully Automated, High Purity Cell Isolation for Chimerism Labs - Educational Materials - Scientific ResourcesFully Automated, High Purity Cell Isolation for Chimerism Labs - Educational Materials - Scientific Resources

2 As patients can show complete chimerism in one cell lineage and partial chimerism in another, lineage-specific chimerism ... HLA Chimerism Whole Blood CD3 Positive Selection Kit (Catalog #17871), and EasySep™ HLA Chimerism Whole Blood Myeloid Positive ... Chimerism analysis can be facilitated with EasySep™, a column-free immunomagnetic cell separation platform, which provides a ... Why Use RoboSep™ for Chimerism Analysis?. *. HIGH PURITY.. Achieve purities of up to 99% in as little as 25 minutes. ...
more infohttps://www.stemcell.com/technical-resources/educational-materials/fully-automated-high-purity-cell-isolation-chimerism-labs.html

Improving the Safety of Tolerance Induction: Chimerism and Cellular Co-Treatment Strategies Applied to Vascularized Composite...Improving the Safety of Tolerance Induction: Chimerism and Cellular Co-Treatment Strategies Applied to Vascularized Composite...

J. Y. Lin, F. C. Tsai, C. G. Wallace, W. C. Huang, F. C. Wei, and S. K. Liao, "Optimizing chimerism level through bone marrow ... W. C. Huang, J. Y. Lin, F. C. Wei, and S. K. Liao, "Induction of tolerance through mixed chimerism for composite tissue ... H. Pan, K. Zhao, L. Wang et al., "Mesenchymal stem cells enhance the induction of mixed chimerism and tolerance to rat hind- ... K. A. Prabhune, V. S. Gorantla, C. Maldonado, G. Perez-Abadia, J. H. Barker, and S. T. Ildstad, "Mixed allogeneic chimerism and ...
more infohttps://www.hindawi.com/journals/jir/2012/107901/ref/

Genetic chimerism in fiction - WikipediaGenetic chimerism in fiction - Wikipedia

Human genetic chimerism, which can not only cause a wide range of illnesses but also lead to the same person having more than ... In Mother 3, chimerism is one of main themes, with chimeric animals being introduced early on in the game. The first chimera ... In the House episode "Cane and Able", a boy who believes he was abducted by aliens is diagnosed with chimerism. In an episode ... One of the subplots in Michael Crichtons 2006 novel Next deals with chimerism. In season three of the TV series ReGenesis, a ...
more infohttps://en.wikipedia.org/wiki/Genetic_chimerism_in_fiction

Phase I/II Pilot Study of Mixed Chimerism to Treat Inherited Metabolic Disorders - Full Text View - ClinicalTrials.govPhase I/II Pilot Study of Mixed Chimerism to Treat Inherited Metabolic Disorders - Full Text View - ClinicalTrials.gov

Phase I/II Pilot Study of Mixed Chimerism to Treat Inherited Metabolic Disorders. This study is ongoing, but not recruiting ... The goal of this research study is to establish chimerism and avoid graft-versus-host-disease (GVHD) in patients with inherited ... The objective for the study is to establish chimerism following reduced intensity conditioning with no grade III/IV GVHD. The ... Phase I/II Pilot Study of Mixed Chimerism to Treat Inherited Metabolic Disorders. ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01372228?term=Niemann-Pick+Disease&rank=18

JCI Insight -
Induced regulatory T cells in allograft tolerance via transient mixed chimerismJCI Insight - Induced regulatory T cells in allograft tolerance via transient mixed chimerism

Since allograft tolerance was achieved in these recipients without durable chimerism, peripheral mechanisms are postulated to ... and humans via induction of transient hematopoietic chimerism. ... T cells in allograft tolerance via transient mixed chimerism. * ... specific induction of iTregs by donor antigens is key to long-term allograft tolerance induced by transient mixed chimerism. ...
more infohttps://insight.jci.org/articles/view/86419/figure/6

Mechanism of origin in two cases of chimerismMechanism of origin in two cases of chimerism

Chimerism is defined as the presence in a subject of more than one stable and genetically distinct cell line; cases reported so ... androgenetic chimerism, different authors suggested different mechanisms resulting in such chimeras. Mechanism of origin ...
more infohttps://file.scirp.org/Html/16386.html

Pulmonary endothelial chimerism after hematopoietic stem cell transplantation, Surgery Today | 10.1007/s00595-017-1562-2 |...Pulmonary endothelial chimerism after hematopoietic stem cell transplantation, Surgery Today | 10.1007/s00595-017-1562-2 |...

"Pulmonary endothelial chimerism after hematopoietic stem cell transplantation, Surgery Today" on DeepDyve, the largest online ... Pulmonary endothelial chimerism after hematopoietic stem cell transplantation. Pulmonary endothelial chimerism after ... Pulmonary endothelial chimerism after hematopoietic stem cell transplantation. Hijiya, Kyoko; Chen-Yoshikawa, Toyofumi; ... Endothelial cell chimerism associated with graft rejection after human lung transplantation. Murata, H; Ratajczak, P; Meignin, ...
more infohttps://www.deepdyve.com/lp/springer_journal/pulmonary-endothelial-chimerism-after-hematopoietic-stem-cell-PFoOc0U52l

Endothelial chimerism in chronic sclerotic-type chronic graft-versus-host disease (GVHD) and GVHD-associated angiomatosis.Endothelial chimerism in chronic sclerotic-type chronic graft-versus-host disease (GVHD) and GVHD-associated angiomatosis.

Homepage , Research & Education , Find a Scientific Publication , Endothelial chimerism in chronic sclerotic-type chronic graft ... Endothelial chimerism in chronic sclerotic-type chronic graft-versus-host disease (GVHD) and GVHD-associated angiomatosis.. ...
more infohttps://cancer.osu.edu/research-and-education/find-a-scientific-publication/endothelial-chimerism-in-chronic-sclerotic-type-chronic-graft-versus-host-disease-gvhd-and-gvhd-associated-angiomatosis
  • Chimerism testing for assessment of stem cell engraftment is performed on genomic DNA extracted from peripheral blood or bone marrow aspirate samples. (mdanderson.org)
  • The pre-transplant chimerism analysis of both donor and recipient is necessary in order to evaluate for the presence of hematopoietic microchimerism in patients at various time points subsequent to allogeneic bone marrow transplant. (uhhospitals.org)
  • The number of alleles in the profile established by the STR panel at the time of the pre-transplant chimerism analysis will determine the complexity of the post-transplant chimerism analysis. (uhhospitals.org)
  • Due to the reduced antitumor effect of TCD grafts, pre-emptive post-transplant donor lymphocyte infusion (DLI) may be needed for treatment of mixed chimerism or persistent disease. (frontiersin.org)
  • In this technical bulletin, we describe a method to automate isolation of lymphoid (T and B cells) and myeloid lineages from human whole blood samples for chimerism analysis. (stemcell.com)
  • The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. (stemcell.com)
  • Chimerism analysis can be facilitated with EasySep™ , a column-free immunomagnetic cell separation platform, which provides a fast and easy method to obtain highly purified cells (Figure 1). (stemcell.com)
  • Chapters in this section focus on the effects of chimerism on testing in relationship determination and forensics, prenatal genetic testing and screening, and blood and HLA typing. (springer.com)
  • Paul Bradbury Kamloops, BC, Canada Kemlo Rogerson wrote: >I have a student that wants to follow a NVQ (National Vocational >Qualification) and has chosen Chimerism (Genetic Mosaicism) about which >I know nowt. (histosearch.com)
  • Human genetic chimerism, which can not only cause a wide range of illnesses but also lead to the same person having more than one profile in genetic fingerprinting, has served as a plot device in many works of fiction. (wikipedia.org)
  • Most known examples are subsequent to the 2004 book Free Culture, where author Lawrence Lessig digresses briefly to describe chimerism and suggest that it could, and had yet to, be well used as a television plot device (particularly for police procedurals involving genetic fingerprinting). (wikipedia.org)
  • The second part of the volume outlines clinical identification and consequences of chimerism. (springer.com)
  • Written by experts in the field, Chimerism: A Clinical Guide is a valuable resource for clinicians and researchers that will help guide patient management and stimulate investigative efforts. (springer.com)
  • In an accompanying editorial, Thomas Starzl, M.D., the University of Pittsburgh liver transplant pioneer, questioned the disappearance of chimerism in these patients. (medpagetoday.com)
  • Tandem Chimerism as a means to increase protein complexity in the human genome Parra et al. (wikipedia.org)
  • The basis for this approach was the observation that red cell chimerism in the majority of dizygotic freemartin cattle twins that shared a common placenta [ 2 ] persisted into adulthood. (omicsonline.org)
  • This text provides a comprehensive, up-to-date review of chimerism. (springer.com)
  • Cells are magnetically labeled directly in the sample with MACSprep Chimerism MicroBeads. (miltenyibiotec.com)
  • Depending on your target cells, you can choose between a broad range of MACSprepTM Chimerism MicroBeads. (miltenyibiotec.com)