Chimerin Proteins: A family of GTPASE-ACTIVATING PROTEINS that are specific for RAC GTP-BINDING PROTEINS.Chimerin 1: A GTPase activating protein that is specific for RAC GTP-BINDING PROTEINS. It is expressed primarily in the brain and may be involved in signal transduction. The alternatively spliced form of CHIMERIN 1 (alpha-2 Chimerin) contains an additional src homology domain and is expressed in both the brain and testes.Proto-Oncogene Proteins c-bcr: Proto-oncogene protein bcr is a serine-threonine kinase that functions as a negative regulator of CELL PROLIFERATION and NEOPLASTIC CELL TRANSFORMATION. It is commonly fused with cellular abl protein to form BCR-ABL FUSION PROTEINS in PHILADELPHIA CHROMOSOME positive LEUKEMIA patients.ras GTPase-Activating Proteins: PROTEINS that specifically activate the GTP-phosphohydrolase activity of RAS PROTEINS.Chromosomes, Human, Pair 2: A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.Spermatocytes: Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.rac GTP-Binding Proteins: A sub-family of RHO GTP-BINDING PROTEINS that is involved in regulating the organization of cytoskeletal filaments. This enzyme was formerly listed as EC 3.6.1.47.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Neurobiology: The study of the structure, growth, activities, and functions of NEURONS and the NERVOUS SYSTEM.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Allergy and Immunology: A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.Membrane Fusion: The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.Viral Fusion Proteins: Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.Cell Fusion: Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.United StatesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Metabolic Networks and Pathways: Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.Computer Graphics: The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.GAP-43 Protein: A nervous tissue specific protein which is highly expressed in NEURONS during development and NERVE REGENERATION. It has been implicated in neurite outgrowth, long-term potentiation, SIGNAL TRANSDUCTION, and NEUROTRANSMITTER release. (From Neurotoxicology 1994;15(1):41-7) It is also a substrate of PROTEIN KINASE C.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Protein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Centrosome: The cell center, consisting of a pair of CENTRIOLES surrounded by a cloud of amorphous material called the pericentriolar region. During interphase, the centrosome nucleates microtubule outgrowth. The centrosome duplicates and, during mitosis, separates to form the two poles of the mitotic spindle (MITOTIC SPINDLE APPARATUS).Quail: Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.Axons: Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.Cysteine: A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.Methanosarcina barkeri: A species of halophilic archaea whose organisms are nonmotile. Habitats include freshwater and marine mud, animal-waste lagoons, and the rumens of ungulates.Semiconductors: Materials that have a limited and usually variable electrical conductivity. They are particularly useful for the production of solid-state electronic devices.Aspergillus oryzae: An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Heterotrimeric GTP-Binding Proteins: GTP-BINDING PROTEINS that contain three non-identical subunits. They are found associated with members of the seven transmembrane domain superfamily of G-PROTEIN-COUPLED RECEPTORS. Upon activation the GTP-BINDING PROTEIN ALPHA SUBUNIT of the complex dissociates leaving a dimer of a GTP-BINDING PROTEIN BETA SUBUNIT bound to a GTP-BINDING PROTEIN GAMMA SUBUNIT.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.GTP-Binding Protein alpha Subunits, Gs: A family of heterotrimeric GTP-binding protein alpha subunits that activate ADENYLYL CYCLASES.Receptors, G-Protein-Coupled: The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.

Molecular heterogeneity and function of EWS-WT1 fusion transcripts in desmoplastic small round cell tumors. (1/21)

Desmoplastic small round cell tumor (DSRCT) is a primitive sarcoma with a consistent cytogenetic abnormality, t(11;22)(p13;q12). This chromosomal translocation generates a chimeric transcript that is formed by fusion of the 5' region of the Ewing's sarcoma gene, EWS, with the 3' DNA-binding segment of WT1, the Wilms' tumor suppressor gene. We collected 14 DSRCT tumor samples and examined the hybrid transcripts. We identified: (a) combinatorial heterogeneity of EWS exons fused to WT1 including use of EWS exons 7, 8, and 9; (b) subpopulations of variant transcripts in 6 of 14 tumors characterized by aberrant splicing resulting in loss of EWS exon 6 or WT1 exon 9; (c) multiple cDNA products with large internal deletions; and (d) insertion of small stretches of heterologous DNA at the fusion site or exon splice region in transcripts from two tumors. Most of the splice variants were in-frame, and in vitro translated fusion proteins with intact DNA-binding motifs formed complexes with a WT1 response element in gel mobility assays. Each of the chimeric proteins retains the ability to bind to the GC and TC elements of the early transcription factor EGR-1 as well as WT1 consensus sequences. We present evidence that various EWS-WT1 proteins up-regulated EGR-1 promoter activity and that this up-regulation is specifically dependent upon the absence of the exon 9 KTS domain of WT1. The molecular diversity and functionality exhibited by these fusion transcripts may have significant biological implications for their transactivating and tumorigenic potential.  (+info)

Adenovirus-mediated transfer of inducible caspases: a novel "death switch" gene therapeutic approach to prostate cancer. (2/21)

In patients with localized prostate cancer, radical prostatectomy and radiation therapy, although effective in controlling localized disease, are often associated with significant side effects attributable to injury of adjacent tissues. Moreover, patients with metastatic disease eventually fail systemic hormonal or chemotherapy because of the development of progressive, refractory disease. In this study, we evaluated the safety and efficacy of a novel suicide gene therapy that could potentially spare normal tissue while bypassing molecular mechanisms of apoptosis resistance by using chemically inducible effector caspases to trigger apoptosis in prostate cancer cells. Initially, we compared the ability of a panel of inducible Fas signaling intermediates to kill human and murine prostate cancer cell lines. On the basis of the superior killing by downstream caspase-1 and caspase-3, replication-deficient adenoviral vectors expressing conditional caspase-1 (Ad-G/iCasp1) or caspase-3 (Ad-G/iCasp3), regulated by nontoxic, lipid-permeable, chemical inducers of dimerization (CID), were constructed. Upon vector transduction followed by CID administration, aggregation and activation of these recombinant caspases occur, leading to rapid apoptosis. In vitro, both human (LNCaP and PC-3) and murine (TRAMP-C2 and TRAMP-C2G) prostate cancer cell lines were efficiently transduced and killed in a CID-dependent fashion. In vivo, direct injection of Ad-G/iCasp1 into s.c. TRAMP-C2 tumors caused focal but extensive apoptosis without evidence for a bystander effect at the maximal viral dose (i.e., 2.5 x 10(10) viral particles/25 microl) in host animals that also received CID compared with control animals. Treatment with Ad-G/iCasp1 plus CID resulted in a transient, yet significant, reduction both in tumor growth and volume compared with tumors treated with vector but not CID (P < 0.035) or vector-diluent plus CID (P < 0.022), both of which grew more rapidly. These results demonstrate that CID-regulated, caspase-based suicide gene therapy is safe and can inhibit the growth of experimental prostate cancer in vitro and in vivo through potent induction of apoptosis, providing a rationale for further development.  (+info)

Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain. (3/21)

The regulation and function of chimaerins, a family of "non-protein kinase C" (PKC) phorbol ester/diacylglycerol receptors with Rac-GAP activity, is largely unknown. In a search for chimaerin-interacting proteins, we isolated Tmp21-I (p23), a protein localized at the perinuclear Golgi area. Remarkably, phorbol esters translocate beta2-chimaerin to the perinuclear region and promote its association with Tmp21-I in a PKC-independent manner. A deletional analysis revealed that the C1 domain in chimaerins is required for the interaction with Tmp21-I, thereby implying a novel function for this domain in protein-protein associations in addition to its role in lipid and phorbol ester binding. Our results support the emerging concept that multiple pathways transduce signaling by phorbol esters and revealed that, like PKC isozymes, chimaerins are subject to a positional regulation. In this setting, Tmp21-I serves as an anchoring protein that determines the intracellular localization of these novel phorbol ester receptors.  (+info)

A receptor for vascular endothelial growth factor that stimulates endothelial apoptosis. (4/21)

Vascular endothelial growth factor (VEGF) is a dimeric angiogenic factor that is overexpressed by many tumors and stimulates tumor angiogenesis. VEGF initiates signaling by dimerizing the receptors VEGFR-1 and VEGFR-2. The Fas receptor stimulates apoptosis, and artificial dimerization of the Fas cytoplasmic domain has been shown to induce apoptosis. We constructed a chimeric receptor (VEGFR2Fas) combining the extracellular and transmembrane domains of VEGFR-2 with the cytoplasmic domain of Fas receptor. When VEGFR2Fas was stably expressed in endothelial cells in vitro, treatment with VEGF rapidly induced cell death with features characteristic of Fas-mediated apoptosis. These findings demonstrate that VEGFR2Fas functions as a VEGF-triggered death receptor and raise the possibility that introduction of VEGFR2Fas into tumor endothelium or tumor cells in vivo may convert tumor-derived VEGF from an angiogenic factor into an antiangiogenesis agent.  (+info)

The exosome pathway in K562 cells is regulated by Rab11. (5/21)

During maturation, reticulocytes lose some membrane proteins that are not required on the mature red cell surface. The proteins are released into the extracellular medium associated with vesicles that are formed by budding of the endosomal membrane into the lumen of the compartment; this process results in the formation of multivesicular bodies (MVBs). Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles, termed exosomes. K562 cells release exosomes with similar characteristics to reticulocyte exosomes, in particular the transferrin receptor (TfR) is found associated with the vesicles. Interestingly, this cell line has been shown to possess high amounts of Rab11 compared with other Rab proteins. To assess the regulation of transferrin receptor release via exosome secretion by Rab11 in this cell type, K562 cells were stably transfected with GFP-Rab11wt or the GTP- and GDP-locked mutants. The distribution of the proteins was assessed by fluorescence microscopy. Transferrin recycling and the number of TfRs present on the surface of the transfected cells were reduced by overexpression of either Rab11wt or the mutants. The amount of released exosomes was analyzed by measuring different molecular markers present on these vesicles either biochemically or by western blot. Overexpression of the dominant-negative mutant Rab11S25N inhibited exosome release, whereas the secretion of exosomes was slightly stimulated in cells transfected with Rab11wt. Taken together, the results demonstrate that in K562 cells Rab11 modulates the exosome pathway although the exact step involved is still not known.  (+info)

Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters. (6/21)

Diacylglycerol is an essential second messenger in mammalian cells. The most prominent intracellular targets of diacylglycerol and of the functionally analogous phorbol esters belong to the protein kinase C (PKC) family. However, at least five alternative types of high-affinity diacylglycerol/phorbol-ester receptor are known: chimaerins, protein kinase D, RasGRPs, Munc13s and DAG kinase gamma. Recent evidence indicates that these have functional roles in diacylglycerol second messenger signalling in vivo and that several cellular processes depend on these targets rather than protein kinase C isozymes. These findings contradict the still prevalent view according to which all diacylglycerol/phorbol-ester effects are caused by the activation of protein kinase C isozymes. RasGRP1 (in Ras/Raf/MEK/ERK signalling) and Munc13-1 (in neurotransmitter secretion) are examples of non-PKC diacylglycerol/phorbol-ester receptors that mediate diacylglycerol and phorbol-ester effects originally thought to be caused by PKC isozymes. In the future, pharmacological studies on PKC must be complemented with alternative experimental approaches to allow the separation of PKC-mediated effects from those caused by alternative targets of the diacylglycerol second messenger pathway. The examples of RasGRP1 and Munc13-1 show that detailed genetic analyses of C(1)-domain-containing non-PKC diacylglycerol/phorbol-ester receptors in mammals are ideally suited to achieve this goal.  (+info)

Enhancing major histocompatibility complex class I antigen presentation by targeting antigen to centrosomes. (7/21)

Several strategies that increase proteasomal degradation of antigen have been shown to improve MHC class I presentation of antigen. Because recent studies have demonstrated that the centrosome is a subcellular compartment rich in proteasomes, we hypothesized that targeting a tumor antigen to centrosomal compartments would enhance both the MHC class I presentation of antigen and the vaccine potency. We, therefore, created a chimera of gamma-tubulin, an established centrosomal marker, with a model tumor antigen, human papillomavirus type 16 (HPV-16) E7, in a DNA vaccine. The linkage of gamma-tubulin to E7-targeted antigen to centrosomal compartments, resulted in enhanced MHC class I presentation of E7, and led to a marked increase in the number of E7-specific CD8(+) T-cell precursors as well as a potent CD4-independent antitumor effect against an E7-expressing tumor cell line, TC-1. In addition, vaccination with gamma-tubulin/E7 DNA in transporter associated with antigen presentation (TAP)-1-knockout mice revealed that the enhancement of E7-specific CD8(+) T-cell immune responses is TAP-1-dependent. Our data suggest that the centrosome may be an important locus for MHC class I antigen processing and that targeting antigen to the centrosome can improve DNA vaccine potency.  (+info)

Targeting protein kinase C and "non-kinase" phorbol ester receptors: emerging concepts and therapeutic implications. (8/21)

Phorbol esters, natural compounds that mimic the action of the lipid second messenger diacylglycerol (DAG), are known to exert their biological actions through the activation of classical and novel protein kinase C (PKC) isozymes. Phorbol esters, via binding to the PKC C1 domains, cause major effects on mitogenesis by controlling the activity of cyclin-cdk complexes and the expression of cdk inhibitors. In the last years it became clear that phorbol esters activate other molecules having a C1 domain in addition to PKCs. One of the most interesting families of "non-kinase" phorbol ester receptors is represented by the chimaerins, lipid-regulated Rac-GAPs that modulate actin cytoskeleton reorganization, migration, and proliferation. The discovery of the chimaerins and other "non-kinase" phorbol ester receptors has major implications in the design of agents for cancer therapy.  (+info)

Roncoroni, A.J.; de Cortigianni, M.R.; García Damiano, M.C., 1989: Cost and effectiveness of fecal culture in the etiologic diagnosis of acute diarrhea
sample_1: CssA4 (36mer)0.4 1.1 mM; CssA4 (36mer), 13C/15N-uniformly labeled, 0.4 1.1 mM; CssA4 (36mer), 13C/15N-AU labeled, 0.4 1.1 mM; CssA4 (36mer), partially deuterated, 0.4 1.1 mM; H2O 95%; D2O 5%. sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 280 K. sample_conditions_2: pH: 6.0; pressure: 1 atm; temperature: 298 K ...
In our globalized world, infection research is becoming ever more important. Thanks to long-distance travel and global exchange of goods, bacteria and viruses can spread very rapidly. Only by learning how these pathogens function will we be able to effectively protect ourselves against them.. Thats the approach pursued by CSSB, where biologists, chemists, medical scientists, physicists and engineers join forces to investigate the interaction of pathogens with their hosts. DESY offers them unique facilities in Germany that provide optimal conditions for structural biology. Using super microscopes such as PETRA III, FLASH and European XFEL, reseachers are able to study biological samples in different ways - ranging from structural analysis of single molecules to real-time imaging of processes in living cells - and thus explore the molecular basis of diseases with extremely high spatial and temporal resolution.. ...
In our globalized world, infection research is becoming ever more important. Thanks to long-distance travel and global exchange of goods, bacteria and viruses can spread very rapidly. Only by learning how these pathogens function will we be able to effectively protect ourselves against them.. Thats the approach pursued by CSSB, where biologists, chemists, medical scientists, physicists and engineers join forces to investigate the interaction of pathogens with their hosts. DESY offers them unique facilities in Germany that provide optimal conditions for structural biology. Using super microscopes such as PETRA III, FLASH and European XFEL, reseachers are able to study biological samples in different ways - ranging from structural analysis of single molecules to real-time imaging of processes in living cells - and thus explore the molecular basis of diseases with extremely high spatial and temporal resolution.. ...
Most of the studies on cell proliferation examine the control of gene expression by specific transcription factors that act on transcriptional initiation. In the last few years, it became evident that mRNA stability/turnover provides an important mechanism for post-transcriptional control of gene expression. In eukaryotes, mRNAs are mainly degraded after deadenylation by decapping and exosome pathways. Mechanisms of mRNA surveillance comprise deadenylation-independent pathways such as NMD (nonsense-mediated decay), when mRNAs harbour a PTC (premature termination codon), NSD (non-stop decay, when mRNAs lack a termination codon, and NGD (no-go decay), when mRNA translation elongation stalls. Many proteins involved in these processes are conserved from bacteria to yeast and humans. Recent papers showed the involvement of proteins deputed to decapping in controlling cell proliferation, virus replication and cell death. In this paper, we will review the newest findings in this field. ...
Our methods of integrating different structural biology approaches and putting molecular complexes into a cellular context can certainly be applied to almost any field, including the field of infection biology. On the systems level, interesting research is also conducted. For example, Jörg Vogel, the director of the Institute for Molecular Infection Biology at University of Würzburg, who will also be talking at the symposium, is investigating the cellular response to bacterial infection and determining how gene expression is changed upon infection. I am looking forward to hearing him speak and to learning more about the different areas in infection biology that require structural biology approaches ...
Complete information for CHN2 gene (Protein Coding), Chimerin 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
You may search for particular presentations by typing key words, an authors name, or the title in the box below. To narrow your search results, prefix your search terms with the day of the week (e.g. Tuesday Corn) ...
Levitra blurred vision - Ebv can also result from a provider with hiv infection, hiv nucleic acid in solution. J am acad child adolesc psychopharmacol. It is suggested otherwise, a hard cervical collar. B. Thiazidesthiazides are distal to the rash.
Recombinant baculoviruses were isolated using the BaculoGold system (Pharmingen) as specified by the manufacturer. HEC-1-A, HeLa, and Vero cells were obtained from American Type Culture Collection (ATCC, Manassas, VA). (ii) pcDNA-MEF-gB, an expression plasmid for gB fused to an MEF tag (MEF-gB), was constructed by cloning a DNA fragment encoding MEF-gB amplified by PCR from recombinant virus DNA expressing MEF-gB (53) into pcDNA4/HisMax C (Invitrogen). To prevent bacterial superinfections, all SCID and RAG1−/−mice received prophylactic treatment of a Sulfatrim pediatric suspension (Barre-National, Baltimore, Md.) at the rate of 5 ml per 200 ml of drinking water. The cell pellets were subsequently lysed in 200 μl of radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 2 mM EDTA, 1 mM Na3VO4, 1 mM phenylmethylsulfonyl fluoride, 1 mM DTT, 10 nM okadaic acid, and Complete protease inhibitor mix). Immunoblotting and ...
Beta-amyloid (Abeta) is a protein fragment created by the cutting of a larger protein, amyloid precursor protein (APP). Beta-amyloid is later secreted from nerve cells and into the fluid space surrounding them, where it aggregates into amyloid plaque, a characteristic feature of Alzheimer pathology. Complex cellular "machinery" and pathways perform the steps involved in secretion of beta-amyloid and other proteins. One such pathway results in the secretion of exosomes, tiny "bubbles" of cellular material released into the space outside the cell. Andrew F. Hill, Ph.D., and colleagues have recently shown that exosomes may contain (1) APP; (2) beta-amyloid, the final product of APP cleavage; and (3) an intermediate portion of APP known as the C-terminal fragment. They also found evidence that some of the cutting of APP to yield beta-amyloid occurs in the exosome.. Dr. Hill and colleagues plan to perform additional studies of the exosome pathway and its role in the processing and secretion of APP ...
Purpose: Myocilin has two distinct cellular distributions, cytoplasmic and membrane-associated, yet appears extracellularly. We have previously shown that myocilin is released from cells on exosomes, which are a component of the endosomal pathway. In this study we tested the hypothesis that myocilin enters the exosome pathway during plasma membrane receptor endocytosis.. Methods: Retinal pigment epithelia (RPE) in human eye-cups was stimulated with L-DOPA (1uM) to monitor endocytosis of GPR143 (OA1), an endogenous G-protein coupled receptor (GPCR) on the surface of RPE. Recombinant myocilin (WT, P370L and T377M) was heterologously co-expressed in transformed cells with GPR143 to test whether myocilin participates in receptor endocytosis and to determine the kinetics of binding. Cell surface proteins in eye-cups and transformed cells were biotinylated and protein trafficking and myocilin association were monitored over time using streptavidin beads. Finally, we tested myocilin binding to the ...
Diverse GTPase-activating proteins (GAPs) for the p21rho subfamily were detected by a novel overlay assay (Manser, E., Leung, T., Monfries, C., Teo, M., Hall, C., and Lim, L. (1992) J. Biol. Chem. 267, 16025-16028), with some GAPs being tissue-specific. Using a PCR strategy exploiting conserved regions common to rho/rac-GAPs, we have isolated a rat testis cDNA encoding a 34-kDa rac-GAP termed beta-chimaerin, as it was highly related to n-chimaerin, containing both a GAP domain (77% identity) and the phorbol ester-binding region (93% identity). beta-Chimaerin mRNA is expressed exclusively in the testis at the onset of sexual maturation. In situ hybridization and cell fractionation analyses show beta-chimaerin mRNA expression to be stage-specific, paralleling acrosomal assembly at the late stage of spermatogenesis. A corresponding testis-specific 30-kDa rac-GAP was detected. The testis-specific and stage-dependent expression of this new member of the chimaerin family offer an alternative model system for
Membership in the Churg Strauss Syndrome Association is free, although donations are always gratefully accepted. Completing the membership form will place you on the CSSA mailing list, a one way communication from the CSSA to members to inform people about updates to the website, news of upcoming conferences and any other news pertaining to CSS related matters.
The CSSA is pleased to share two new patient stories by David Hohmann and Denise Trachsel. Both David and Denise are well known on the Churg Strauss message boards for their …
This mesmerizing soft pink rose quartz ball is seated delicately on top of a wooden stand. Rose Quartz is one of the most desirable of all quartz and is highly valued for the harmonious soft vibes it radiates; rose quartz is a powerful love energizer and is associated with love, romance, and relationships. The rose quartz spheres semi-translucent pink color and beautiful swirls makes it an excellent ornament for your home or office.. Rose Quartz Application and Symbolism in Feng Shui Also known as "love stone", rose quartz are highly treasured for their soft harmonious vibes, and their use as a powerful love energizer. This rose quartz is a lovely gift because it is a strong symbol of love, and provides calm, delicate inspiration for its owner. The rose quartz is known to speed up processes of finding love, mend emotional wounds, strengthen relationships, and help with comprehension of love.. The rose quartzs delicate vibes make it a popular tool for emotional healing in meditation as well. ...
... , Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
β2-chimaerin is a Rac1-specific negative regulator and a candidate tumor suppressor in breast cancer but its precise function in mammary tumorigenesis in vivo is unknown. Here, we study for the first time the role of β2-chimaerin in breast cancer using a mouse model and describe an unforeseen role for this protein in epithelial cell-cell adhesion. We demonstrate that expression of β2-chimaerin in breast cancer epithelial cells reduces E-cadherin protein levels, thus loosening cell-cell contacts. In vivo, genetic ablation of β2-chimaerin in the MMTV-Neu/ErbB2 mice accelerates tumor onset, but delays tumor progression. Finally, analysis of clinical databases revealed an inverse correlation between β2-chimaerin and E-cadherin gene expressions in Her2+ breast tumors. Furthermore, breast cancer patients with low β2-chimaerin expression have reduced relapse free survival but develop metastasis at similar times. Overall, our data redefine the role of β2-chimaerin as tumor suppressor and provide ...
Living Mulch Builds Profits, Soil Living mulch functions like mulch on any farm or garden except - its alive. No, its not out of the latest horror movie; living mulch is a system farmers can use to benefit both profits and the soil. While the system has been around for a whil.... - American Society of Agronomy (ASA), Crop Science Society of America (CSSA), Soil Science Society of America (SSSA). Agronomy Journal, August 10, 2017. Embargo expired on 18-Oct-2017 at 09:00 ET ...
Given the forty year history of the program, the models in use have evolved over time and are captured in PANACHE under two broad categories. CURRENT MODELS describe models and flowcharts that are currently used in the program. LEGACY MODELS describe models that have been used previously in the ETSP but are presently not part of our core testing strategy. The overall intent is to provide accurate information on tests used for compounds in PANAChE, regardless of when that testing occurred ...
A range of conditions see table . Due to uid inux into brain cells caused by cigar viagra either active contraction process must be aware that clindamycin resistance must exceed the risks. If mri is the request for organtissue donation has a history of fever history of. Recognition of sepsis in healthy children with inborn errors manifest themselves in one injury. We can affirm that fact that anxiety is panic attacks., a panic attack a discrete etiologic diagnosis. J osteopath med korr im.
Global antibody supplier and research reagent supplier to the life science community. Find antibodies and reagents all backed by our Guarantee+.
For the first time, an entire publication has been dedicated to providing a critical review of the identification and analysis of the milk specific proteins
I am not a medical or health professional. Information in this site has been gathered from numerous sources and is for research purposes only. I do not guarantee the accuracy of any information in this site. Desmoplastic Small Round Cell Tumor www.DSRCT.com ...
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
I am a pediatric oncologist who specializes in the treatment of children and young adults with neuroblastoma and other solid tumors, such as desmoplastic small round cell tumors. These are challenging cancers to treat. By caring for patients and conducting research, I hope to be able make an impact and improve the lives of people diagnosed with these diseases.
Jays Tips. Peri-Colace seems to work better than Colace for constipation brought on by cancer medications. ww.dsrct.com desmoplastic small round cell tumor. ...
A Makahu father with a rare form of cancer is being denied a drug that may give him more time with his four young children. James Grieve, 44, was diagnosed with Desmoplastic Small Round Cell …
Expert Rev Allti Illfect Tiler. 98 -0. EBV can occa- sionally be transmitted through blood transfusion or bone marrow transplantation. 53 Belli P, Costantini M, Mirk P, et al.
Desmoplastic small round cell tumor (DSRCT) is a rare, biologically aggressive soft tissue neoplasm of uncertain differentiation, most often arising in the abdominal and pelvic cavities of adolescents and young adults with a striking male predominance. Histologically, it is characterized by islands of uniform small round cells in prominent desmoplastic stroma, and it has a polyimmunophenotypic profile, typically expressing WT1 and cytokeratin, desmin, and neural/neuroendocrine differentiation markers to varying degrees. Tumors at other sites and with variant morphology are more rarely described. DSRCT is associated with a recurrent t(11;22)(p13;q12) translocation, leading to the characteristic EWSR1-WT1 gene fusion. Fluorescence in situ hybridization (FISH), to detect EWSR1 rearrangement, and reverse transcription-polymerase chain reaction (RT-PCR) to assess for EWSR1-WT1 fusion transcripts are routine diagnostic ancillary tools. We present a large institutional comparative series of FISH and ...
Patient, family, or caregivers personal pages, journals, experiences with Desmoplastic small round cell cancer. Also known as DSRCT, Desmoplastic sarcoma, Desmoplastic small round blue tumor
A rare, aggressive sarcoma that often develops in the abdominal cavity, although it can also arise elsewhere. The disease commonly affects adolescents and young adults, mostly male. Its name means that it is formed by small, round cancer cells surrounded by scar-like tissue.
Wang LL, Perlman EJ, Vujanic GM, Zuppan C, Brundler MA, Cheung CR, Calicchio ML, Dubois S, Cendron M, Murata-Collins JL, Wenger GD, Strzelecki D, Barr FG, Collins T, Perez-Atayde AR, Kozakewich H. Desmoplastic small round cell tumor of the kidney in childhood. Am J Surg Pathol. 2007 Apr; 31(4):576-84. View abstract ...
1997). "Human myosin-IXb, an unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail". ... Saeki N, Tokuo H, Ikebe M (2005). "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein ... Myosin-IXb is a protein that in humans is encoded by the MYO9B gene. GRCh38: Ensembl release 89: ENSG00000099331 - Ensembl, May ...
The gene sal-like 4 (SALL4) or CHN1 (chimerin) has became a mutated gene (protein) and it is also one of the cause of the ...
... chimerin 1 MeSH D12.776.402.150.500.460 - neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH ... groel protein MeSH D12.776.602.500.500.100 - fusion proteins, bcr-abl MeSH D12.776.602.500.500.320 - fusion proteins, gag-onc ... oncogene protein v-maf MeSH D12.776.964.700.750.875 - oncogene proteins v-abl MeSH D12.776.964.700.750.882 - oncogene proteins ... fusion proteins, gag-pol MeSH D12.776.964.775.350.400 - hiv core protein p24 MeSH D12.776.964.775.375.325 - fusion proteins, ...
Chimerin, a type of nerve tissue protein City Hindus Network, a non profit-making organisation that promotes charity, ...
... is a type of nerve tissue protein. Types include: Chimerin 1 Chimerin 2 Chimerin Proteins at the US National Library ...
... , (CHN1) also known as alpha-1-chimerin, n-chimerin is a protein which in humans is encoded by the CHN1 gene. ... Chimerin 1 is a GTPase activating protein specific for RAC GTP-binding proteins. It is expressed primarily in the brain and may ... "Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and ... CHN1 is a three-domain protein with the N-terminal SH2 domain, the C-terminal RhoGAP domain and the central C1 domain similar ...
This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain ... 2005). "A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with ... This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol ( ... DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the ...
Agrin Chimerin Proteins Chromogranins Dopamine and cAMP-Regulated Phosphoprotein 32 Fragile X Mental Retardation Protein GAP-43 ... Neuronal Apoptosis-Inhibitory Protein Neuronal Calcium-Sensor Proteins Neuropeptides Olfactory Marker Protein S100 Proteins ... Neuronal apoptosis inhibitory protein (NAIP) belongs to the family of proteins called the inhibiter of apoptosis family (IAP), ... Protein Glucose Transporter Type 3 Hu Paraneoplastic Encephalomyelitis Antigens Microtubule-Associated Proteins Myelin Proteins ...
... cdc2 protein kinase MeSH D12.776.476.325.150.100 -- chimerin proteins MeSH D12.776.476.325.150.100.200 -- chimerin 1 MeSH ... smad1 protein MeSH D12.776.476.024.417.500.200 -- smad2 protein MeSH D12.776.476.024.417.500.300 -- smad3 protein MeSH D12.776. ... smad proteins, inhibitory MeSH D12.776.476.024.417.249.600 -- smad6 protein MeSH D12.776.476.024.417.249.700 -- smad7 protein ... gtp-binding protein alpha subunits, g12-g13 MeSH D12.776.476.375.100.200 -- gtp-binding protein alpha subunits, gi-go MeSH ...
... regulate G proteins in several different ways. Small GTPases act as molecular switches in ... and thus requires another class of regulatory proteins to accelerate this activity, the GTPase activating proteins (GAPs). ... GTP-Binding+Protein+Regulators at the US National Library of Medicine Medical Subject Headings (MeSH) ... The inactive form of GTPases (GDP-form) are activated by a class of proteins called Guanosine nucleotide exchange factors (GEFs ...
Growth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene.[5] ... neuron growth-associated protein 43. Function[edit]. GAP43, is a nervous tissue-specific cytoplasmic protein that can be ... protein kinase C-activating G-protein coupled receptor signaling pathway. • response to wounding. • regulation of growth. • ... protein-protein interaction and structural remodeling via actin polymerization.. Clinical significance[edit]. Humans with a ...
... gtpase-activating proteins MeSH D12.644.360.325.150.100 --- chimerin proteins MeSH D12.644.360.325.150.100.200 --- chimerin 1 ... rap gtp-binding proteins MeSH D12.644.360.525.475.100 --- rap1 gtp-binding proteins MeSH D12.644.360.525.500 --- ras proteins ... grb2 adaptor protein MeSH D12.644.360.024.298 --- grb7 adaptor protein MeSH D12.644.360.024.300 --- grb10 adaptor protein MeSH ... 14-3-3 proteins MeSH D12.644.360.024.318 --- proto-oncogene proteins c-crk MeSH D12.644.360.024.326 --- proto-oncogene proteins ...
Parathyroid hormone-related protein. *Peroxisome proliferator-activated receptor gamma. *Pleiotrophin. *RAB7A. *RET proto- ... Chimerin 2. *Α-シヌクレイン. *CD4. Metadata. This file contains additional information, probably added from the digital camera or ...
Protein Coding), Chimerin 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Protein Symbol:. P52757-CHIO_HUMAN. Recommended name:. Beta-chimaerin Protein Accession:. P52757. Secondary Accessions: *A4D1A2 ... CHN2 (Chimerin 2) is a Protein Coding gene. Diseases associated with CHN2 include Hepatosplenic T-Cell Lymphoma and Background ... chimerin beta-2,GTPase activating protein for the ras-related p21rac,DAG (diacylglycerol) receptor,expressed in cerebellum, ...
GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity ... The Gene Wiki collection of pages on human genes and proteins. More...GeneWikii. Chimerin_2. ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Pfam protein domain database. More...Pfami. View protein in Pfam. PF00130 C1_1, 1 hit. PF00620 RhoGAP, 1 hit. PF00017 SH2, 1 ...
Chimerin is a type of nerve tissue protein. Types include: Chimerin 1 Chimerin 2 Chimerin Proteins at the US National Library ...
This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain ... 2005). "A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with ... This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol ( ... DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the ...
The Chimaerin gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, an SH2 ... This protein has GTPase-activating protein activity which is regulated by phospholipid binding and binding of diacylglycerol ( ... DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
1996) Human myosin IX-b. An unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail. J ... 1997) Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines. Proc Natl Acad Sci USA 94:9956- ... Myr 8 antibody-Protein G Sepharose. Two milligrams of affinity-purified myr 8b antibodies were coupled to Protein G Sepharose 4 ... 1988) The Drosophila ninaC locus encodes two photoreceptor cell-specific proteins with domains homologous to protein kinases ...
Caltag Medsystems supply a wide range of fusion proteins for cancer research, neurobiology, metabolism, apoptosis, cell biology ... Fusion Protein (10ug). FP-0007. Signosis. Enquire. order. Beta-chimaerin (Beta-chimerin) (SH2-Domain - CHN2 ). Fusion Protein ( ... Fusion Protein (10ug). FP-0061. Signosis. Enquire. order. 70 kDa SHP-1L protein. (SH2-Domain - SHP1L-D1). Fusion Protein (10ug) ... Fusion Protein (10ug). FP-0086. Signosis. Enquire. order. Adapter protein GRID. (SH2-Domain - GRID). Fusion Protein (10ug). FP- ...
1997). "Human myosin-IXb, an unconventional myosin with a chimerin-like rho/rac GTPase-activating protein domain in its tail". ... Saeki N, Tokuo H, Ikebe M (2005). "BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein ... Myosin-IXb is a protein that in humans is encoded by the MYO9B gene. GRCh38: Ensembl release 89: ENSG00000099331 - Ensembl, May ...
... and Cdc42 effector protein 4 gene increased in expression, whereas Arhgap18 and N-chimerin decreased. Several genes the ... The corresponding human protein was identified as an associated protein of the tumor suppressor hDlg and is an in vitro ... Among these are Cyclins B1, D1, D2, the Cell division control protein 2 (Cdc2/p34), and the Cell division cycle protein 20 ( ... Microtubule motor proteins fulfill a specialized function in motor cilia and flagella. In addition, these proteins mediate the ...
Chimerin 1. Protein Coding. 16.22. DISEASES inferred 15 GeneCards inferred via :. DISEASES inferred (show sections) ...
Crystal structure of human Rho GTPase activating protein 15 (ARHGAP15). 3cxl. Crystal structure of human chimerin 1 (CHN1). ... are GTPase activating proteins (GAPs) for members of the Rho subfamily of small GTP-binding proteins (G proteins or GTPases). A ... GAP proteins inactivate small Rho and Ras proteins, so inactivation of rhoGAP proteins might cause constitutive activation of ... Click on the protein counts, or double click on taxonomic names to display all proteins containing RhoGAP domain in the ...
Regulation of chimerin proteins occurs in response to growth factor receptor or G-protein coupled receptor activation followed ... is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in ... is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in ... β-arrestin binding uncouples the receptor from the G protein, thereby terminating G protein-mediated signaling (desensitization ...
Chimerin 1, (CHN1) also known as alpha-1-chimerin, n-chimerin is a protein which in humans is encoded by the CHN1 gene. ... Chimerin 1 is a GTPase activating protein specific for RAC GTP-binding proteins. It is expressed primarily in the brain and may ... "Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and ... CHN1 is a three-domain protein with the N-terminal SH2 domain, the C-terminal RhoGAP domain and the central C1 domain similar ...
N-chimaerin (NC) (N-chimerin) (Alpha chimerin) (A-chimaerin) (Rho GTPase-activating protein 2). ... U4/U6.U5 tri-snRNP-associated protein 3 (U4/U6.U5 tri-snRNP-associated 27 kDa protein) (27K) (Nucleic acid-binding protein RY-1 ... Structure: While protein structure does not always reveal much about the function of a gene, such information is often highly ... Motif Matches: A motif or domain match for some portion of the protein. The number and characteristics of each motif or domain ...
... protein_coding gene_symbol:Chn1 description:chimerin (chimaerin) 1 ... cdna:known chromosome:VEGA66:2:73610660:73775346:-1 gene:OTTMUSG00000013428 gene_biotype:protein_coding transcript_biotype: ...
The gene sal-like 4 (SALL4) or CHN1 (chimerin) has became a mutated gene (protein) and it is also one of the cause of the ...
... chimerin 1 MeSH D12.776.402.150.500.460 - neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH ... groel protein MeSH D12.776.602.500.500.100 - fusion proteins, bcr-abl MeSH D12.776.602.500.500.320 - fusion proteins, gag-onc ... oncogene protein v-maf MeSH D12.776.964.700.750.875 - oncogene proteins v-abl MeSH D12.776.964.700.750.882 - oncogene proteins ... fusion proteins, gag-pol MeSH D12.776.964.775.350.400 - hiv core protein p24 MeSH D12.776.964.775.375.325 - fusion proteins, ...
Agrin Chimerin Proteins Chromogranins Dopamine and cAMP-Regulated Phosphoprotein 32 Fragile X Mental Retardation Protein GAP-43 ... Neuronal Apoptosis-Inhibitory Protein Neuronal Calcium-Sensor Proteins Neuropeptides Olfactory Marker Protein S100 Proteins ... Neuronal apoptosis inhibitory protein (NAIP) belongs to the family of proteins called the inhibiter of apoptosis family (IAP), ... Protein Glucose Transporter Type 3 Hu Paraneoplastic Encephalomyelitis Antigens Microtubule-Associated Proteins Myelin Proteins ...
Chimerin, a type of nerve tissue protein City Hindus Network, a non profit-making organisation that promotes charity, ...
Chimerin 1. Protein Coding. 518.64. Pathogenic 6 Genetic Tests 29 GeneCards inferred via :. Variants (show sections) 17197532 ... An important gene associated with Duane Syndrome Type 2 is CHN1 (Chimerin 1). Affiliated tissues include eye. ...
α-chimaerin (α-chimerin), a Rac-specific GTPase activating protein, plays a key role to wire neurons precisely in the brain. ...
Chimerin Proteins / blood. Dose-Response Relationship, Drug. Female. Humans. Male. Middle Aged. Young Adult ... Neoplasm Proteins; 0 / Proto-Oncogene Proteins c-bcl-2; EC 2.7.10.1 / Protein-Tyrosine Kinases; EC 2.7.10.1 / Receptor Protein- ... Microtubule-Associated Proteins / metabolism. Neoplasm Proteins / metabolism. Proto-Oncogene Proteins c-bcl-2 / metabolism. ... Protein-Tyrosine Kinases / genetics. Protein-Tyrosine Kinases / metabolism. Receptor Protein-Tyrosine Kinases ...
Chimerin 1. Protein Coding. 418.36. Causative germline mutation 60 DISEASES inferred 15 ... An important gene associated with Duane Retraction Syndrome is CHN1 (Chimerin 1). Affiliated tissues include heart, skin and t ...
GEM interacting protein. NM_016573. Gene Info. GNAO1. Guanine nucleotide binding protein (G protein), alpha activating activity ... Chimerin 1. NM_001822. NM_001025201. NM_001206602. Gene Info. CNN3. Calponin 3, acidic. NM_001839. Gene Info. ... Regulator of G-protein signaling 1. NM_002922. Gene Info. RGS10. Regulator of G-protein signaling 10. NM_001005339. NM_002925. ... Regulator of G-protein signaling 22. NM_015668. Gene Info. RGS3. Regulator of G-protein signaling 3. NM_144488. NM_130795. NM_ ...
... beta chimerin; RhoGAP3; beta-chimerin; beta3-chimaerin; rho-GTPase-activating protein 3; BCH; CHN2-3; MGC138360 ... This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain ... This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol ( ... DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the ...
  • This is a sub-part (intracellular signaling peptides and proteins only) of List of MeSH codes (D12.776), itself a part of the list of the "D" codes for MeSH. (wikipedia.org)
  • They can take many forms, including soluble and cell‐bound ligands (proteins, lipids, peptides or small molecules) and insoluble matrix substrates, but to act as guidance cues, they must be presented to the cell in a spatially restricted manner. (embopress.org)
  • Previous studies have revealed genetic interactions between RGD1 and the SLG1 and MID2 genes, encoding two putative sensors for cell integrity signaling, and VRP1 encoding an actin and myosin interacting protein involved in polarized growth. (sdbonline.org)
  • A gene or protein consisting of parts from two different genes or proteins that are normally distinct, sometimes derived from two different species. (thefreedictionary.com)
  • For example, the genes control the synthesis of structural proteins and also the enzymes that regulate various chemical reactions that take place in a cell. (thefreedictionary.com)
  • the induction of the genes encoding zona pellucida protein 3 and a nucleoside diphosphate kinase (nm23). (biomedcentral.com)
  • In the present review, we have identified some of the most significant protein encoding genes whose mutations have great impact in the development of diabetic condition. (geneticsmr.org)
  • Washington DC, USA ---------------------------------------------------------------------------- Description: Human chromosome 7: entries, gene names and cross-references to MIM Name: humchr07.txt Release: 2018_10 of 07-Nov-2018 ---------------------------------------------------------------------------- This documents lists all the human protein sequence entries whose genes are known to be encoded on chromosome 7 in this release of UniProtKB/Swiss-Prot. (uniprot.org)
  • The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. (cancerindex.org)
  • Waetzig, Seegert, Rosenstiel, Nikolaus, Schreiber: p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease. (anticorps-enligne.fr)
  • Signal-transducing protein phosphorylation cascades mediated by Ras/Rho proteins in the mammalian cell: the potential for multiplex signalling. (bio-rad.com)
  • Its important role in memory and information storage is executed through its cell biological mechanisms of phosphorylation, palmitoylation , protein-protein interaction and structural remodeling via actin polymerization. (wikipedia.org)
  • PLC[gamma]1 is an enzyme which upon phosphorylation to its active form cleaves the membrane phospholipid, phosphatidylinositol 4, 5 biphosphate (PIP2) to diacylglycerol (DAG) and inositol triphosphate (IP3), which regulate intracellular calcium stores and protein kinase C levels (2). (thefreedictionary.com)
  • Preconditioning causes the activation of various receptors (adenosine A1 receptors, opioid receptors and bradykinin receptors) and the generation of other free radicals, which then transmit signals via G proteins, which lead to the formation of inositol triphosphate, production of diacylglycerol and cause the activation of protein kinases, phosphorylation of ADP and the enhancement of [K. (thefreedictionary.com)
  • The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. (harvard.edu)
  • This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. (genecards.org)
  • p>This subsection of the 'Function' section specifies the position(s) and type(s) of zinc fingers within the protein. (uniprot.org)
  • The interaction between NAIP and hippocalcin, a neuronal calcium-sensor protein, has been observed to take place in the zinc-binding region along with other specific amino acids. (wikipedia.org)
  • Bcr expression levels are relatively constant throughout mouse development until adulthood in brain and in hematopoietic tissues, a pattern that is distinctly different from that of the functionally related n-chimerin gene. (lu.se)
  • These data demonstrated that CXCL5 expression was upregulated in prostate cancer tissues and that exogenous CXCL5 protein exposure or CXCL5 overexpression promoted malignant phenotypes of prostate cancer cells in vitro and in vivo. (anticorps-enligne.fr)
  • They act upstream of the protein kinase C (PKC) pathway and are thought to monitor the state of the cell surface and relay the information to Pkc1p. (sdbonline.org)
  • Pkc1p activates a mitogen-activated protein (MAP) kinase cascade, named the PKC pathway, consisting of Bck1p, Mkk1p/Mkk2p, and the MAP kinase Mpk1p. (sdbonline.org)
  • The protein Slg1 is linked to the PKC pathway by the finding that this MAP kinase cascade is activated by heat stress via Slg1p. (sdbonline.org)
  • The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. (bio-rad.com)
  • One of the postulated mechanisms of action of Benfotiamine is that it blocks three major pathways (the hexosamine pathway, the advanced glycation end product formation pathway, and the diacylglycerol (DAG)-protein kinase C (PKC) pathway) by probable removal of glyceryl aldehyde 3-phosphate and fructose 6-phosphate through the activation of the pentos phosphate enzyme transketolase. (thefreedictionary.com)
  • We present a comprehensive list of 54 C1 domains occurring singly or doubly in 34 different proteins. (embl.de)
  • Residues conserved across the rhoGAP family are largely confined to one face of this bundle, which may be an interaction site for target G proteins. (embl-heidelberg.de)
  • A family of homologous proteins of low MOLECULAR WEIGHT that are predominately expressed in the BRAIN and that have been implicated in a variety of human diseases. (harvard.edu)
  • Classical protein kinase C (PKC) family members are activated by the binding of various ligands to one of several cysteine-rich domains of the enzyme. (embl.de)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • These domains were first discovered as the loci of phorbol ester and diacylglycerol binding to conventional protein kinase C isozymes, which contain 2 C1 domains (C1A and C1B) in their N-terminal regulatory regions. (embl.de)
  • Many C1 domains and C1 domain-containing proteins bind phorbol esters, but many others do not. (embl.de)
  • NMR structure of a protein kinase C-gamma phorbol-binding domain and study of protein-lipid micelle interactions. (embl.de)