Recombinant Fusion Proteins
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Mutant Chimeric Proteins
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Sequence Homology, Amino Acid
Bone Marrow Transplantation
Green Fluorescent Proteins
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Host vs Graft Reaction
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Protein Structure, Secondary
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Receptors, Purinergic P2X1
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Bone Marrow Cells
Fetal Tissue Transplantation
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Amino Acid Motifs
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Major Histocompatibility Complex
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Amino Acid Substitution
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Protein Sorting Signals
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Lysosome-Associated Membrane Glycoproteins
Viral Envelope Proteins
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Mrj encodes a DnaJ-related co-chaperone that is essential for murine placental development. (1/3469)We have identified a novel gene in a gene trap screen that encodes a protein related to the DnaJ co-chaperone in E. coli. The gene, named Mrj (mammalian relative of DnaJ) was expressed throughout development in both the embryo and placenta. Within the placenta, expression was particularly high in trophoblast giant cells but moderate levels were also observed in trophoblast cells of the chorion at embryonic day 8.5, and later in the labyrinth which arises from the attachment of the chorion to the allantois (a process called chorioallantoic fusion). Insertion of the ROSAbetageo gene trap vector into the Mrj gene created a null allele. Homozygous Mrj mutants died at mid-gestation due to a failure of chorioallantoic fusion at embryonic day 8.5, which precluded formation of the mature placenta. At embryonic day 8.5, the chorion in mutants was morphologically normal and expressed the cell adhesion molecule beta4 integrin that is known to be required for chorioallantoic fusion. However, expression of the chorionic trophoblast-specific transcription factor genes Err2 and Gcm1 was significantly reduced. The mutants showed no abnormal phenotypes in other trophoblast cell types or in the embryo proper. This study indicates a previously unsuspected role for chaperone proteins in placental development and represents the first genetic analysis of DnaJ-related protein function in higher eukaryotes. Based on a survey of EST databases representing different mouse tissues and embryonic stages, there are 40 or more DnaJ-related genes in mammals. In addition to Mrj, at least two of these genes are also expressed in the developing mouse placenta. The specificity of the developmental defect in Mrj mutants suggests that each of these genes may have unique tissue and cellular activities. (+info)
The cardiac homeobox gene Csx/Nkx2.5 lies genetically upstream of multiple genes essential for heart development. (2/3469)Csx/Nkx2.5 is a vertebrate homeobox gene with a sequence homology to the Drosophila tinman, which is required for the dorsal mesoderm specification. Recently, heterozygous mutations of this gene were found to cause human congenital heart disease (Schott, J.-J., Benson, D. W., Basson, C. T., Pease, W., Silberbach, G. M., Moak, J. P., Maron, B. J., Seidman, C. E. and Seidman, J. G. (1998) Science 281, 108-111). To investigate the functions of Csx/Nkx2.5 in cardiac and extracardiac development in the vertebrate, we have generated and analyzed mutant mice completely null for Csx/Nkx2.5. Homozygous null embryos showed arrest of cardiac development after looping and poor development of blood vessels. Moreover, there were severe defects in vascular formation and hematopoiesis in the mutant yolk sac. Interestingly, TUNEL staining and PCNA staining showed neither enhanced apoptosis nor reduced cell proliferation in the mutant myocardium. In situ hybridization studies demonstrated that, among 20 candidate genes examined, expression of ANF, BNP, MLC2V, N-myc, MEF2C, HAND1 and Msx2 was disturbed in the mutant heart. Moreover, in the heart of adult chimeric mice generated from Csx/Nkx2.5 null ES cells, there were almost no ES cell-derived cardiac myocytes, while there were substantial contributions of Csx /Nkx2.5-deficient cells in other organs. Whole-mount &bgr;-gal staining of chimeric embryos showed that more than 20% contribution of Csx/Nkx2. 5-deficient cells in the heart arrested cardiac development. These results indicate that (1) the complete null mutation of Csx/Nkx2.5 did not abolish initial heart looping, (2) there was no enhanced apoptosis or defective cell cycle entry in Csx/Nkx2.5 null cardiac myocytes, (3) Csx/Nkx2.5 regulates expression of several essential transcription factors in the developing heart, (4) Csx/Nkx2.5 is required for later differentiation of cardiac myocytes, (5) Csx/Nkx2. 5 null cells exert dominant interfering effects on cardiac development, and (6) there were severe defects in yolk sac angiogenesis and hematopoiesis in the Csx/Nkx2.5 null embryos. (+info)
Accelerated accumulation of somatic mutations in mice deficient in the nucleotide excision repair gene XPA. (3/3469)Inheritable mutations in nucleotide excision repair (NER) genes cause cancer-prone human disorders, such as xeroderma pigmentosum, which are also characterized by symptoms of accelerated ageing. To study the impact of NER deficiency on mutation accumulation in vivo, mutant frequencies have been determined in liver and brain of 2-16 month old NER deficient XPA-/-, lacZ hybrid mice. While mutant frequencies in liver of 2-month old XPA-/-, lacZ mice were comparable to XPA+/-, lacZ and the lacZ parental strain animals, by 4 months of age mutant frequencies in the XPA-deficient mice were significantly increased by a factor of two and increased further until the age of 16 months. In brain, mutant frequencies were not found to increase with age. These results show that a deficiency in the NER gene XPA causes an accelerated accumulation of somatic mutations in liver but not in brain. This is in keeping with a higher incidence of spontaneous liver tumors reported earlier for XPA-/- mice after about 15 months of age. (+info)
Ectopic expression of the transforming growth factor beta type II receptor disrupts mesoderm organisation during mouse gastrulation. (4/3469)Transforming growth factor beta (TGFbeta) regulates the cell cycle and extracellular matrix (ECM) deposition of many cells in vitro. We have analysed chimaeric mouse embryos generated from embryonic stem cells with abnormal receptor expression to study the effect of TGFbeta on these processes in vivo and the consequences for normal development. The binding receptor for TGFbeta, TbetaRII, is first detected in the embryo proper around day 8.5 in the heart. Ectopic expression of TbetaRII from the blastocyst stage onward resulted in an embryonic lethal around 9.5 dpc. Analysis of earlier stages revealed that the primitive streak of TbetaRII chimaeras failed to elongate. Furthermore, although cells passed through the streak and initially formed mesoderm, they tended to accumulate within the streak. These defects temporally and spatially paralleled the expression of the TGFbeta type I receptor, which is first expressed in the node and primitive streak. We present evidence that classical TGFbeta-induced growth inhibition was probably the cause of insufficient mesoderm being available for paraxial and axial structures. The results demonstrate that (1) TGFbeta mRNA and protein detected previously in early postimplantation embryos is present as a biologically active ligand; and (2) assuming that ectopic expression of TbetaRII results in no other changes in ES cells, the absence of TbetaRII is the principle reason why the embryo proper is unresponsive to TGFbeta ligand until after gastrulation. (+info)
Comparative analysis of cell distribution in the pigment epithelium and the visual cell layer of chimaeric mice. (5/3469)In chimaeras of both rdrdCC in equilibrium ++ cc and rdrdcc in equilibrium ++CC combinations two types of distribution were observed. In a majority of the chimaeras both retinal layers were chimaeric; whereas in a few cases the pigment epithelium was chimaeric but the visual cell layer was made of ++ cells only. No spatial relation was observed in the distribution of the cells in the two layers. The two eyes of the individuals were nearly always identical with regard to occurrence of chimaerism in the two layers. The findings are discussed in the light of the possible site and mode of expression of the rd gene. (+info)
Marmoset species variation in the humoral antibody response: in vivo and in vitro studies. (6/3469)A comparison of the in vivo and in vitro antibody response capabilities of two marmoset species, Saguinus fuscicollis and Saguinus oedipus oedipus, revealed the former to be superior in elaborating humoral antibody. In vivo challenges with Escherichia coli lipopolysaccharide (LPS) and Salmonella typhi flagella consistently yielded higher antibody titres in S. fuscicollis; indeed, with LPS antigen, multiple inoculations of S.o. oedipus marmosets led ultimately to a decrease in antibody formation, in contrast to the anamnestic response of S. fuscicollis. This species differential in immune competence was also suggested in the in vitro stimulation of peripheral blood leucocytes (PBL) and spleen cells with sheep red blood cells (RBC). None of 55 S.o. oedipus PBL cultures and 49 of 89 (55%) S. fuscicollis cultures responded to the test antigen. A similar differential in response to sheep RBC was noted with the spleen cells of each species, although this report contrasts the antibody-forming potential of two marmoset species, a comparison of the immunological response profile of marmosets to those of other laboratory animals challenged with similar antigens suggests these primates may be relatively incompetent. The possible relationship between the haemopoietic chimerism of marmosets and a diminished immune competence is discussed. (+info)
Bmp4 is required for the generation of primordial germ cells in the mouse embryo. (7/3469)In many organisms the allocation of primordial germ cells (PGCs) is determined by the inheritance of maternal factors deposited in the egg. However, in mammals, inductive cell interactions are required around gastrulation to establish the germ line. Here, we show that Bmp4 homozygous null embryos contain no PGCs. They also lack an allantois, an extraembryonic mesodermal tissue derived, like the PGCs, from precursors in the proximal epiblast. Heterozygotes have fewer PGCs than normal, due to a reduction in the size of the founding population and not to an effect on its subsequent expansion. Analysis of beta-galactosidase activity in Bmp4(lacZneo) embryos reveals that prior to gastrulation, Bmp4 is expressed in the extraembryonic ectoderm. Later, Bmp4 is expressed in the extraembryonic mesoderm, but not in PGCs. Chimera analysis indicates that it is the Bmp4 expression in the extraembryonic ectoderm that regulates the formation of allantois and primordial germ cell precursors, and the size of the founding population of PGCs. The initiation of the germ line in the mouse therefore depends on a secreted signal from the previously segregated, extraembryonic, trophectoderm lineage. (+info)
Interaction of B cells with activated T cells reduces the threshold for CD40-mediated B cell activation. (8/3469)CD154-CD40 interactions are of central importance for the induction of antibody responses to T-dependent antigens. Since most anti-CD40 mAb are only weak B cell mitogens, it is believed that under physiological conditions, signals through CD40 synergize with those from other receptors on B cells to induce B cell activation. We show here that the interaction of either normal B cells, or those from CBA/N (xid) mice, with CD3-activated primary T cells in whole spleen cell cultures markedly reduces the threshold for B cell activation via CD40. Hence, these pre-activated cells undergo vigorous proliferation when stimulated with either optimal or suboptimal concentrations of weakly mitogenic anti-CD40 mAb, or with soluble CD40 ligand. Blocking experiments indicate that the establishment of this priming effect requires stimulation via CD40 itself, plus T cell-derived IL-2. In support of this concept, only CD3/CD28-pre-activated, but not CD3-pre-activated T cells induce this effect, unless the co-cultures of B cells with the latter T cells are supplemented with IL-2. Although B cells activated in this fashion do express higher levels of CD40 than naive cells, we believe that this is insufficient to explain the observed dramatic effects on their proliferative capacity. Rather we propose that T cell-dependent B cell activation induces fundamental changes in the signalling machinery invoked by ligation of CD40. It is likely that this amplification loop could play an important role during the initiation of antibody responses to T-dependent antigens, when activated CD4 T cells only express low levels of CD154. (+info)
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... is a moth in the family Geometridae. It is found in Mexico. Yu, Dicky Sick Ki. "Eupithecia chimera (Dyar ... 2003). "Eupithecia chimera". The Global Lepidoptera Names Index. Natural History Museum. Retrieved May 3, 2018. v t e (LepIndex ...
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In mythology, a chimera is a creature such as a hippogriff or a gryphon formed from parts of different animals, thus the name ... A chimera or chimeric virus is a virus that contains genetic material derived from two or more distinct viruses. It is defined ... Other viral chimeras have also been found, and the group is known as the CHIV viruses ("chimeric viruses"). Combining two ... The study surprised scientists, because DNA and RNA viruses vary and the way the chimera came together was not understood. ...
The term genetic chimera has been used at least since the 1944 article of Belgovskii. An animal chimera is a single organism ... Animal chimeras are produced by the merger of two (or more) embryos. In plant chimeras, however, the distinct types of tissue ... Most chimeras will go through life without realizing they are chimeras. The difference in phenotypes may be subtle (e.g., ... These are chimeras in which the layers differ in their chromosome constitution. Occasionally, chimeras arise from loss or gain ...
In paleontology, a chimera is a fossil that was reconstructed with elements coming from more than a single species or genus of ... 1, p. 1-84 v t e (Articles with short description, Short description is different from Wikidata, Paleontological chimeras, All ... A now classic example of chimera is Protoavis. Archaeoraptor Dalianraptor? Lametasaurus? Protoavis Piltdown Man Ultrasauros ...
The Chimera (/kɪˈmɪərə/ or /kaɪˈmɪərə/), also Chimaera (Chimæra) (Ancient Greek: Χίμαιρα, Chímaira means 'she-goat'), according ... This outline of Chimera motifs follows Marilyn Low Schmitt, "Bellerophon and the Chimaera in Archaic Greek Art" American ... Look up chimera in Wiktionary, the free dictionary. Wikimedia Commons has media related to Chimera. Theoi Project: Khimaira ... It is not known what the Indus people called the Chimera.  Although the Chimera of antiquity was forgotten in ...
3) #31 (November 2009) JSA All-Stars #14 (March 2011) King Chimera at DC Wikia King Chimera at Comic Vine (Articles lacking ... King Chimera is a fictional superhero in the DC Comics Universe, a member of the superhero team the Justice Society of America ... King Chimera was first portrayed as an arrogant, egotistical, classy "know-it-all" during his run in Justice Society of America ... King Chimera first appeared in Justice Society of America (vol. 3) #24 (April 2009), and was created by Lilah Sturges and ...
Chimera is a NASA mission concept to orbit and explore 29P/Schwassmann-Wachmann 1 (SW1), an active, outbursting small icy body ... The Chimera proposal was ranked in the first tier (Category 1) of submissions, but was not selected for further development for ... Chimera will be the first orbital exploration of an outer solar system small body and the third orbital spacecraft mission ( ... A series of gravity assist maneuvers are used to position Chimera at SW1 with a relative velocity low enough to permit orbital ...
The Western Kimberley tree gehyra (Gehyra chimera) is a species of gecko. It is endemic to Western Australia. "Gehyra chimera ...
The Chimera Painter (also spelled Chimaera Painter) was an anonymous Corinthian black figure vase painter active c. 600-575 B.C ... Media related to Chimera Painter at Wikimedia Commons Plate with Seated Lioness on Google Arts and Culture Corinthian Round- ... "Chimera Painter". Getty Museum Collection. J. Paul Getty Museum. Retrieved 30 August 2022. *Amnéus, Cynthia; Forth, Ron; Hisey ... He is named for the Chimera depicted on one of his works, which is now in Vienna. The artist was likely a student of the ...
The Chimera House (also known as the "13 floor money back house") is an urban legend which consists of the usual young teens ... Although the Chimera House has never been proven to exist, some people still search fervently for it and several haunted ... The legend of the Chimera House has been fueled by both hoaxes and haunted attractions that tried to cash in on the legend by ... The anthology Haunted Houses edited by Robert D. San Souci contains a short story inspired by the legend called "Chimera House ...
Chimera tells the story of Sara Janning, a woman who carves away massive chunks of ice as part of a tritium mining operation on ... Chimera is an American comic book published by CrossGen Entertainment from March to July 2003. It is a mini-series that ran for ... Chimera is most notable for its artwork. Produced by Brandon Peterson, the artwork is a blend of traditional and computer- ... "Brok Chimeran - Crossgen Comics - Chimera - Character profile". Writeups.org. 2015-02-01. Retrieved 2022-08-24. DAVE School ...
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"Chimera - Single". Retrieved 31 January 2015. "Music". Marie Digby Official Website. Retrieved 31 January 2015. "Chimera - New ... Chimera is an EP by Marié Digby. It was released on August 16, 2014. "Vanish in the Air" is the single of Marie Digby's EP ... Chimera. This EP was first featured at iTunes Store. The EP contains three new Marié Digby songs. This EP is a distinct ...
Chimera was re-signed a two-year contract extension with the Capitals on September 29, 2011. Chimera was re-signed a two-year ... "Chimera Agrees to Two-Year Deal". NHL. 2016-07-01. Retrieved 2016-07-01. "Ducks Acquire Chimera from Islanders in Exchange for ... "Caps agree to term on new contract with Jason Chimera". NHL. 2011-09-29. Retrieved 2011-09-29. "Capitals re-sign Jason Chimera ... Chimera was subsequently dealt to the Columbus Blue Jackets, without playing a game in Phoenix, as part of a trade for Geoff ...
... is a Kenyan writer. He received the Noma Award in 2000 for Ufundishaji wa Fasihi: Nadharia na Mbinu. Chimera ...
... is an unreleased coin-operated arcade video game developed by C.P. Brain and planned to be released by Jaleco in ... Chimera Beast takes place on a planet which is described as distant and Earth-like. The planet is overrun by monsters known as ... Chimera Beast runs on dual 68000 processors. Sound is provided by Yamaha YM2151 and dual OKI MSM6295 chips. Like most games of ... The enemies in Chimera Beast are mostly animals, some with equivalent Earth versions and some without. Some of the enemy ...
The Chimera's Curse
The serpent-like Chimera is only a small part of his deadly army. As the dangerous fire of Kullervo's hatred bursts into life, ... The Chimera's Curse is a children's fantasy novel by British writer Julia Golding, first published in 2007. It is the fourth ... create the Chimera, a monster with three heads, one for each lion, goat and serpent. Colin (Col) Clamworthy: about 14 years old ...
Chimera of Arezzo
... www.getty.edu/art/exhibitions/chimaera/chimaera_checklist.pdf[bare URL PDF] Hesiod, Theogony 319-25 "The Chimaera of Arezzo: ... "The Chimaera of Arezzo (Getty Villa Exhibitions)". www.getty.edu. Retrieved 2017-12-27. Becker, Jeffery A. "Chimera of Arezzo ( ... Typical iconography of the Chimera myth depicts the warrior Bellerophon as he confronts the Chimera, or rides atop or alongside ... Wikimedia Commons has media related to Chimera of Arezzo. Ugo Bardi, 1997. "The Chimaera of Arezzo" (All articles with bare ...
Chimera (Barth novel)
Chimera is written in three loosely related sections, or novellas, similar to the way the mythical Chimera is a hybrid creature ... Chimera is a 1972 fantasy novel written by American writer John Barth, composed of three loosely connected novellas. The ... Chimera. New York: Random House, 1972. John Barth's Acceptance Speech for the 1973 National Book Award (Articles with short ... Bellerophoniad Bellerophon: The Greek mythic hero who first tamed Pegasus and slayed the Chimera, becoming the King of Lycia. ...
Chimera (software library)
Chimera documentation by Rama Alebouyeh included with source code Chimera at UCSB v t e (Distributed data storage, All stub ... Chimera is currently being used in industry labs, as part of research done by the U.S. Department of Defense, and by startup ... Chimera is a software library created as a research project at UCSB for the C programming language that implements a structured ... "Chimera, a Structured Peer-to-Peer Overlay". Archived from the original on 2006-08-24. Retrieved 2008-07-08. ...
Chimera (molecular biology)
Some computational methods have been devised to detect and remove chimeras, like: CHECK_CHIMERA of the Ribosomal Database ... "Chimeras". www.drive5.com. Retrieved 2022-10-27. Edgar, Robert C. (2016-09-12). "UCHIME2: improved chimera prediction for ... is now considered an artefact based on a chimera of CYP2C18 and CYP2C19." Researchers have created receptor chimeras in their ... A chimera is a novel sequence that will most probably not match to any known organism. Hence, it might be interpreted as a new ...
Chimera (Andromeda album)
Chimera is the third album by the progressive metal band Andromeda, released in 2006. The album was reviewed and received ...
Chimera (larp convention)
Chimera is a Live action role-playing (LARP) convention held annually every August in Auckland, New Zealand. The convention is ... "Having a larp", 20/20 report about Chimera 2009 "Playing the Larp Part", NZ Life & Leisure, November 2013 (Defunct gaming ...
Chimera (2001 film)
Roberto Nepoti (April 3, 2001). "La 'Chimera' di Corsicato". La Repubblica. Retrieved 6 March 2014. Chimera at IMDb Rotten ... Chimera is a 2001 Italian romance film directed by Pappi Corsicato. One night in bed a husband tells his wife about a couple he ...
Italian corvette Chimera
Chimera has been borne by at least two corvettes of the Italian Navy and may refer to: Italian corvette Chimera (1943), a ... Gabbiano-class corvette launched in 1943 Italian corvette Chimera (F 556), a Minerva-class corvette launched in 1990 This ...
Chimera (Russian band)
Chimera". Chimera's influences include Bauhaus, Nico, Butthole Surfers, Sonic Youth, Fugazi, Daniil Kharms, Jules Verne, Helena ... Chimera's songs have been covered by artists and bands such as Psychea, Pilot, Iva Nova, Zakhar May, and PTVP. Alexei Nikonov [ ... Chimera (also known as Deputat Baltiki) was a Russian underground rock band formed by singer/guitarist Edward Starkov [ru] and ... Chimera repeatedly gave concerts in Germany. In February 1997, at the age of 27, Starkov hanged himself in the attic of ...
Snake Robots And A Quadcopter Fuse Together In This Chimera Drone
Nashashibi/Skaer: Chimera | University of Dundee
Wall vinyl reads Chimera Nashashibi/Skaer Gallery Thumbnail - Video projection of a lamb in a lambing shed Gallery Thumbnail - ... Nashashibi/Skaer: Chimera Friday 30 September 2022 - Saturday 10 December 2022 An exhibition of new and existing works by ... Chimera: Levitating Tongues. Thursday 20 October 2022, 18:00 - 19:30. Vocal performance by Ceylan Hay and Shiori Usui with a ... The three films in Chimera, Our Magnolia (2009), Lamb (2019) and Bear (2021) subtly examine different aspects of our perceptual ...
Jason Chimera (LW) Odds & Betting Lines - New York Islanders - Yahoo Sports
GateWorld » Chimera (715) - sg1 715 184 - Stargate Image Gallery
Lessons from Chimeras | The Scientist Magazine®
The chimera marmoset story reported linkurl:here;http://www.the-scientist.com/news/home/53033/ and elsewhere is fascinating; I ... Lessons from Chimeras. The chimera marmoset story reported linkurl:here;http://www.the-scientist.com/news/home/53033/ and ... were both actually XX/XY chimeras of each other. The twins developed, DNA analysis suggests, from a single egg... ... Another interesting chimera story, this one in humans, linkurl:was noted in the media;http://www.nature.com/news/2007/070326/ ...
Browsing by Subject "Transplantation Chimera"
Mycobacterium chimaera Contamination of Heater-Cooler Devices Used in Cardiac Surgery - United States | MMWR
Mycobacterium chimaera Contamination of Heater-Cooler Devices Used in Cardiac Surgery - United States. MMWR Morb Mortal Wkly ... These results strongly suggest a point-source contamination of Stöckert 3T heater-cooler devices with M. chimaera. A recent ... Mycobacterium chimaera infections associated with Sorin Group Deutschland GmBH Stöckert 3T Heater-Cooler System: FDA Safety ... Notes from the Field: Mycobacterium chimaera Contamination of Heater-Cooler Devices Used in Cardiac Surgery - United States. ...
Chimera Archives ⋆ Mythical Realm
The Chimera [ki-meer-uh, kahy-], or Chimaera as its sometimes known, was said to be a hybrid of three creatures. This amazing ... Achilles Apollo Bellerophon Centaur Cerberus Chimera Chiron Egyptian Mythology Elves Event Goddess Greek Mythology Gryphon ... The dreaded Chimera took up residence in Caria, one of Greeces neighbors and trade partners. Great havoc did it cause, killing ...
Chimera Investment Corporation Announces Date of First Quarter 2023 Financial Results and Conference Call | Business Wire
Chimera Investment Corporation Announces Date of First Quarter 2023 Financial Results and Conference Call ... About Chimera Investment Corporation We are a publicly traded REIT that is primarily engaged in the business of investing ... NEW YORK--(BUSINESS WIRE)--Chimera Investment Corporation (NYSE: CIM) (the "Company") announced today that it plans to release ...
etymologia: Chimera (ki-mir′ə) - Volume 14, Number 11-November 2008 - Emerging Infectious Diseases journal - CDC
etymologia: Chimera (ki-mir′ə). Volume 14, Number 11-November 2008. Article Views: 279. Data is collected weekly and does not ... Chimera also refers to a substance created from proteins or genes of 2 species, as by genetic engineering. Chimerism is rare in ... From the Greek Khimaira, Latin Chimaera; she-goat. In Greek mythology: a composite creature with the body and head of a lion, a ... etymologia: Chimera (ki-mir′ə). Emerging Infectious Diseases. 2008;14(11):1789. doi:10.3201/eid1411.e11411.. ...
Chimera N. Thompson | People | K&L Gates
Chimera Thompson is a special projects lawyer in the firms Washington, D.C. office, and is a member of the Power practice ... Chimera counsels clients on a wide range of federal energy regulatory and administrative litigation matters before the Federal ... Prior to joining the firm, Chimera served as an associate lawyer at a national law firm based in Washington, D.C. There, ... Chimera defended clients in criminal and civil enforcement matters before FERC, the Office of Foreign Assets Control (OFAC), ...
Olympus (7) Chimera (Acıgöl, Turkey) - Travellerspoint Travel Photography
Biotinylated Recombinant Human PD-L2-Fc Chimera (carrier-free), PD-L2
When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL ... When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL ... When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC50 of 3 - 12 ng/mL ... Stability testing for Biotinylated Recombinant Human PD-L2-Fc Chimera. Biotinylated Recombinant Human PD-L2-Fc Chimera was ...
Chimera Video Pro Plus 1 Bank (small), NO 8124 - Studio Depot
Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF 2264-S6-050: R&D Systems
Reviews for Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF. There are currently no reviews for this product. Be the ... Have you used Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF?. Submit a review and receive an Amazon gift card.. $25/€ ... 5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of ... first to review Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF and earn rewards! ...
GSM-Forum - View Single Post - Chimera TooL Successfully Unlocked / Repaired / Flashed Phones !! PLEASE POST HERE
Venus the Two Faced Chimera Kitty Cat Leaves New York
New video with Venus the two faced chimera cat shows her relaxing with her petparents after her appearance on the today show, ... Venus the Two Faced Chimera Kitty Cat Leaves New York. September 6, 2012. by Karen Harrison Binette ... New video with Venus the two faced chimera cat shows her relaxing with her petparents after her appearance on the today show, ...
Theros - Chimera Fond Du Lac
A model carries gold and black assemble with great panache, during 'Chimera' fashion show, hosted by WLC College, in Mumbai on...
Chimera fashion show, hosted by WLC College, in Mumbai on December 18, 2012. ... See more of : hot model alecia raut, chimera fashion shows, alecia raut ramp pics. hot model alecia raut, chimera fashion shows ... See more of : hot model alecia raut, chimera fashion shows, alecia raut ramp pics. hot model alecia raut, chimera fashion shows ... See more of : mumbai celebs events, hot indian models, chimera fashion shows. mumbai celebs events, hot indian models, chimera ...
EARNEST / GARRISON / SCOTT Chimera on ImportCDs
Victorian Chimera Lion-Eagle Cast Iron Sculpture Statue
Chimera Skin Scrunch Bikini Bottom (FINAL SALE) - Maya Swimwear
Inspired by the Arabian Nights, the Chimera evokes the essences of mischief, valor, ribaldry and romance. . Front lined . High ... Decrease quantity for Chimera Skin Scrunch Bikini Bottom (FINAL SALE) Increase quantity for Chimera Skin Scrunch Bikini Bottom ... Chimera Skin Scrunch Bikini Bottom (FINAL SALE). Chimera Skin Scrunch Bikini Bottom (FINAL SALE) ... Inspired by the Arabian Nights, the Chimera evokes the essences of mischief, valor, ribaldry and romance. . Front lined . High ...
Chimeras; Mad Scientist Creations or Forbidden Government Experiments? | The Paranormal Explorers
Could these beings be Chimeras?? Something that has skipped the evolutionary chain? Chimeras are a part of mythology; creatures ... Scientific American published an article on human-animal chimeras. The article states that Canada has banned chimera ... Chimeras are a part of mythology; creatures that were part goat, lion and serpent. But now there is talk of using stem cell ... Chimeras; Mad Scientist Creations or Forbidden Government Experiments?. Posted by Paranormal Rona on July 20, 2014 ...
Chalet Chimera, Klosters | Leo Trippi
Within walking distance of the the ski lifts and centre of Klosters, Chalet Chimera combines elegance with traditional styling. ... Built in 2005, Chalet Chimera combines a traditional style with light wood and understated colours. The chalet itself sleeps 10 ... Chalet Chimera combines elegance with traditional styling. ...
Alice Rohrwacher debuts her latest enchantment, 'La Chimera,' at the Cannes Film Festival | iNFOnews | Thompson-Okanagan's News...
"La Chimera," though, isnt just about the gravitational pull of the past. It concerns a merry band of male tombaroli in pursuit ... "La Chimera," a radiant and melancholy highlight of this years Cannes, concludes what Rohrwacher considers a triptych of films ... "What we do with our past is my big question," Rohrwacher said in an interview in Cannes a few days before the "La Chimera" ... "La Chimera," which co-stars Carol Duarte, Isabella Rossellini and Rohrwachers sister, Alba Rohrwacher, is a work of social ...
The chimera of elections in Libya - GIS Reports
- In the spring of 2015, investigators in Switzerland reported a cluster of six patients with invasive infection with Mycobacterium chimaera , a species of nontuberculous mycobacterium ubiquitous in soil and water. (cdc.gov)
- Similar to the Swiss report, a field investigation by the Pennsylvania Department of Health, with assistance from CDC, used both epidemiologic and laboratory evidence to identify an association between invasive Mycobacterium avium complex, including M. chimaera, infections and exposure to contaminated Stöckert 3T heater-cooler devices, all manufactured by LivaNova PLC (formerly Sorin Group Deutschland GmbH) ( 2 ). (cdc.gov)
- New information indicates that these devices, manufactured by LivaNova PLC (formerly Sorin Group Deutschland GmbH), were likely contaminated with the rare bacteria Mycobacterium chimaera during manufacturing. (cdc.gov)
- CDC assisted in a field investigation that used both epidemiologic and laboratory evidence to identify an association between invasive Mycobacterium avium complex (including M. chimaera ) infections and exposure to contaminated 3T heater-cooler devices, consistent with the Swiss report (2). (cdc.gov)
- Although heater-cooler devices are used to regulate patients' blood temperature during cardiopulmonary bypass through water circuits that are closed, these reports suggest that aerosolized M. chimaera from the devices resulted in the invasive infections ( 1 , 2 ). (cdc.gov)
- These results strongly suggest a point-source contamination of Stöckert 3T heater-cooler devices with M. chimaera . (cdc.gov)
- A recent report from Germany noted that preliminary typing results of M. chimaera from heater-cooler devices from three different European countries were almost identical to samples obtained from the manufacturing site, further supporting the likelihood of point-source contamination ( 4 ). (cdc.gov)
- CDC in collaboration with National Jewish Health completed a whole-genome sequencing analysis and results demonstrate that M. chimaera isolates from patients with heater-cooler associated infections and from the 3T heater-cooler devices from several U.S. hospitals (in Pennsylvania and Iowa) are all highly related to each other (3). (cdc.gov)
- This evidence for likely point-source contamination of the 3T heater-cooler devices is consistent with recent reports from Europe ( http://www.fda.gov/MedicalDevices/Safety/AlertsandNotices/ucm504213.htm External ) that describe matching of M. chimaera sequences from environmental isolates at the device production site in Germany and isolates from patients and devices in Europe. (cdc.gov)
- Simultaneous inhibition in cultured cells of their enzymatic activities with the small molecule inhibitors apilimod dimesylate and nafamostat mesylate synergistically prevent viral entry and infection of native SARS-CoV-2 and vesicular stomatitis virus (VSV)-SARS-CoV-2 chimeras expressing the SARS-CoV-2 surface spike (S) protein and of variants of concern. (bvsalud.org)
- The three films in Chimera, Our Magnolia (2009), Lamb (2019) and Bear (2021) subtly examine different aspects of our perceptual universe. (dundee.ac.uk)
- NEW YORK--( BUSINESS WIRE )--Chimera Investment Corporation (NYSE: CIM) (the "Company") announced today that it plans to release its financial results for the first quarter ended March 31, 2023 on Thursday, May 4, 2023 prior to 7:00 a.m. (businesswire.com)
- Director Alice Rohrwacher poses for photographers upon arrival at the premiere of the film 'La Chimera' at the 76th international film festival, Cannes, southern France, Friday, May 26, 2023. (infotel.ca)
- Fusion and slippages of meaning produce an associative and discursive mediation on transformation through which chimera, a composite being that in its very form unsettles the possibility of an archetype, encourages us to doubt the dominance of the real. (dundee.ac.uk)
- however, these reports suggest that M. chimaera can be aerosolized by the devices and result in infections (1,2). (cdc.gov)
- At the heart of Chimera are three collaborative films which combine photographic images with musical composition, drawing and painting, skewing the images to form new experiences. (dundee.ac.uk)
- Chimera Thompson is a special projects lawyer in the firm's Washington, D.C. office, and is a member of the Power practice group. (klgates.com)
- Chimera counsels clients on a wide range of federal energy regulatory and administrative litigation matters before the Federal Energy Regulatory Commission (FERC), including RTO/ISO generation and transmission interconnection issues, market-based rates under Section 205 of the Federal Power Act (FPA), Section 206 complaint proceedings, and FERC enforcement investigations. (klgates.com)
- Our Chimera, the mythological fire-breathing monster, has been a symbol of power since ancient times. (ancientsculpturegallery.com)
- In making "La Chimera," Rohrwacher accompanied a group on an archeological dig. (infotel.ca)
- REFERENCE 1 AUTHORS Motomura,K., Boonchan,M., Noda,M., Tanaka,T. and Takeda,N. CONSRTM Norovirus Surveillance Group of Japan TITLE Norovirus epidemics caused by new GII.2 chimera viruses in 2012-2014 in Japan JOURNAL Infect. (cdc.gov)
- New video with Venus the two faced chimera cat shows her relaxing with her petparents after her appearance on the today show, and before leaving New York to go back home to Florida. (lifewithcats.tv)
- There are currently no international standard governing chimera experiments. (paranormalexplorers.com)
- When human PD-1-Fc Chimera is immobilized at 1.0 µg/mL, biotinylated human PD-L2-Fc Chimera binds with an EC 50 of 3 - 12 ng/mL. (biolegend.com)
- Stability testing for Biotinylated Recombinant Human PD-L2-Fc Chimera. (biolegend.com)
- Biotinylated Recombinant Human PD-L2-Fc Chimera was aliquoted in PBS, pH 7.2, 5% glycerol at 0.2 mg/mL. (biolegend.com)
- The samples were tested in a binding assay with human PD-1-Fc Chimera. (biolegend.com)
- 5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF (Catalog # 256-GF ). (rndsystems.com)
- Scientific American published an article on human-animal chimeras. (paranormalexplorers.com)
- Canada's Assisted Human Reproduction Act of 2004 banned human-animal chimeras. (paranormalexplorers.com)
- The US has no formal restrictions, but Senator Sam Brownback of Kansas proposed legislation in March that would outlaw several kinds of chimeras, including ones with substantial human brain tissue. (paranormalexplorers.com)
- It urged a ban on any use of human cells in other primates, however, as well as the introduction of animal cells into human blastocysts.It also warned against allowing human-animal chimeras to breed: some human cells might have managed to infiltrate the animals' testes and ovaries. (paranormalexplorers.com)
- Built in 2005, Chalet Chimera combines a traditional style with light wood and understated colours. (leotrippi.com)
- In this article, the author seeks to find subsidies on the theme of reverie , in order to deepen the understanding of the motivating elements that result in the appearance of strange images in the analyst's mind during analysis, such as affective pictograms (ROCHA BARROS, 2000), chimeras and paradoxical thoughts (M'UZAN, 1976). (bvsalud.org)
- The article states that Canada has banned chimera experiments, but the US has no such restrictions. (paranormalexplorers.com)
- The NAS recommended that chimeras involving most animal species generally be permitted. (paranormalexplorers.com)
- UCSF Chimera is a highly extensible program for interactive visualization and analysis of molecular structures and related data, including density maps, supramolecular assemblies, sequence alignments, docking results, trajectories, and conformational ensembles. (nih.gov)
- Please see https://www.cgl.ucsf.edu/chimera/docs/credits.html for details on how to cite UCSF Chimera. (nih.gov)
- This video demonstrates how to make a mutation and accommodate said mutation using UCSF Chimera. (nih.gov)
- Former Hubert H. Humphrey Fellow (2008-2009) Adrian Octavian Abagiu, M.D., Ph.D., from Romania has been chosen as one of three recipients of the Chimera Awards 2022, which were awarded by the European Opiate Addiction Treatment Association in May 2022. (nih.gov)
- Preliminary data shows that the WN/Zika chimeras reduce the gold standard plaque neutralization reduction Ab assay testing time from 6 to 4 days. (nih.gov)
- In response to the 2015-2016 Zika outbreak, CDC researchers developed new Zika virus chimeras that can be used for inactivated Zika vaccine candidates and faster Zika antibody (Ab) neutralization assay testing. (nih.gov)
- Here, we report chimera states in electrocorticography recordings preceding, by several hours, each of seven seizures in one patient with epilepsy. (nih.gov)
- Of 97 patients from whom M. chimaera sp. (cdc.gov)
- Create a file with the Chimera commands you want to run. (nih.gov)
- This Funding Opportunity Announcement (FOA) invites applications for pre-clinical and translational research using human cell-mice chimera animals to study HIV pathology in functional live brain environments, with the presence of abused substances. (nih.gov)
- Single nucleotide polymorphisms (SNPs) were identified after comparing patient and device samples against sequence data from an M. chimaera reference isolate. (cdc.gov)