Chemical Fractionation: Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cell Fractionation: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.Fractionation, Field Flow: Separation of molecules and particles by a simultaneous action of carrier liquid flow and focusing field forces (electrical, sedimentation, or thermal), without a stationary phase.Subcellular Fractions: Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Molecular Weight: The sum of the weight of all the atoms in a molecule.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Kinetics: The rate dynamics in chemical or physical systems.Chemistry Techniques, Analytical: Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Multiple Chemical Sensitivity: An acquired disorder characterized by recurrent symptoms, referable to multiple organ systems, occurring in response to demonstrable exposure to many chemically unrelated compounds at doses below those established in the general population to cause harmful effects. (Cullen MR. The worker with multiple chemical sensitivities: an overview. Occup Med 1987;2(4):655-61)Textbooks as Topic: Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.Microwaves: That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.Electric Impedance: The resistance to the flow of either alternating or direct electrical current.Plethysmography, Impedance: Recording changes in electrical impedance between electrodes placed on opposite sides of a part of the body, as a measure of volume changes in the path of the current. (Stedman, 25th ed)Electric Capacitance: The ability of a substrate to retain an electrical charge.Flocculation: The aggregation of suspended solids into larger clumps.Electromagnetic Phenomena: Characteristics of ELECTRICITY and magnetism such as charged particles and the properties and behavior of charged particles, and other phenomena related to or associated with electromagnetism.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Polyethylene: A vinyl polymer made from ethylene. It can be branched or linear. Branched or low-density polyethylene is tough and pliable but not to the same degree as linear polyethylene. Linear or high-density polyethylene has a greater hardness and tensile strength. Polyethylene is used in a variety of products, including implants and prostheses.Polyethylenes: Synthetic thermoplastics that are tough, flexible, inert, and resistant to chemicals and electrical current. They are often used as biocompatible materials for prostheses and implants.Polyethylene Glycols: Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.Lipotropic Agents: Endogenous factors or drugs that increase the transport and metabolism of LIPIDS including the synthesis of LIPOPROTEINS by the LIVER and their uptake by extrahepatic tissues.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Oils, Volatile: Oils which evaporate readily. The volatile oils occur in aromatic plants, to which they give odor and other characteristics. Most volatile oils consist of a mixture of two or more TERPENES or of a mixture of an eleoptene (the more volatile constituent of a volatile oil) with a stearopten (the more solid constituent). The synonym essential oils refers to the essence of a plant, as its perfume or scent, and not to its indispensability.Syntaxin 1: A neuronal cell membrane protein that combines with SNAP-25 and SYNAPTOBREVIN 2 to form a SNARE complex that leads to EXOCYTOSIS.Clathrin: The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.Presynaptic Terminals: The distal terminations of axons which are specialized for the release of neurotransmitters. Also included are varicosities along the course of axons which have similar specializations and also release transmitters. Presynaptic terminals in both the central and peripheral nervous systems are included.Synaptic Vesicles: Membrane-bound compartments which contain transmitter molecules. Synaptic vesicles are concentrated at presynaptic terminals. They actively sequester transmitter molecules from the cytoplasm. In at least some synapses, transmitter release occurs by fusion of these vesicles with the presynaptic membrane, followed by exocytosis of their contents.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.SNARE Proteins: A superfamily of small proteins which are involved in the MEMBRANE FUSION events, intracellular protein trafficking and secretory processes. They share a homologous SNARE motif. The SNARE proteins are divided into subfamilies: QA-SNARES; QB-SNARES; QC-SNARES; and R-SNARES. The formation of a SNARE complex (composed of one each of the four different types SNARE domains (Qa, Qb, Qc, and R)) mediates MEMBRANE FUSION. Following membrane fusion SNARE complexes are dissociated by the NSFs (N-ETHYLMALEIMIDE-SENSITIVE FACTORS), in conjunction with SOLUBLE NSF ATTACHMENT PROTEIN, i.e., SNAPs (no relation to SNAP 25.)Qa-SNARE Proteins: A subfamily of Q-SNARE PROTEINS which occupy the same position as syntaxin 1A in the SNARE complex and which also are most similar to syntaxin 1A in their AMINO ACID SEQUENCE. This subfamily is also known as the syntaxins, although a few so called syntaxins are Qc-SNARES.Lignans: A class of dibenzylbutane derivatives which occurs in higher plants and in fluids (bile, serum, urine, etc.) in man and other animals. These compounds, which have a potential anti-cancer role, can be synthesized in vitro by human fecal flora. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Butylene Glycols: 4-carbon straight chain aliphatic hydrocarbons substituted with two hydroxyl groups. The hydroxyl groups cannot be on the same carbon atom.Flax: A plant genus of the family LINACEAE that is cultivated for its fiber (manufactured into linen cloth). It contains a trypsin inhibitor and the seed is the source of LINSEED OIL.GlucosidesSesamum: A plant genus of the family PEDALIACEAE that is the source of the edible seed and SESAME OIL.Linseed Oil: The fixed oil obtained from the dried ripe seed of linseed, Linum usitatissimum (L. Linaceae). It is used as an emollient in liniments, pastes, and medicinal soaps, and in veterinary medicine as a laxative. It is also called flaxseed oil. (Dorland, 28th ed)HMGA2 Protein: An AT-hook-containing (AT-HOOK MOTIFS) nuclear protein that is expressed predominantly in proliferating and undifferentiated mesenchymal cells.Grape Seed Extract: Exudate from seeds of the grape plant Vitis vinifera, composed of oils and secondary plant metabolites (BIOFLAVONOIDS and polyphenols) credited with important medicinal properties.Proanthocyanidins: Dimers and oligomers of flavan-3-ol units (CATECHIN analogs) linked mainly through C4 to C8 bonds to leucoanthocyanidins. They are structurally similar to ANTHOCYANINS but are the result of a different fork in biosynthetic pathways.Vitis: A plant genus in the family VITACEAE, order Rhamnales, subclass Rosidae. It is a woody vine cultivated worldwide. It is best known for grapes, the edible fruit and used to make WINE and raisins.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Phosgene: A highly toxic gas that has been used as a chemical warfare agent. It is an insidious poison as it is not irritating immediately, even when fatal concentrations are inhaled. (From The Merck Index, 11th ed, p7304)Wheat Germ Agglutinins: Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.Triticum: A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.Hexanols: Isomeric forms and derivatives of hexanol (C6H11OH).Butanols: Isomeric forms and derivatives of butanol (C4H9OH).Enterococcaceae: A family of gram-positive bacteria in the order Lactobacillales, phylum Firmicutes.1-Butanol: A four carbon linear hydrocarbon that has a hydroxy group at position 1.Aminocaproates: Amino derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the amino caproic acid structure.Caproates: Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.Alcohols: Alkyl compounds containing a hydroxyl group. They are classified according to relation of the carbon atom: primary alcohols, R-CH2OH; secondary alcohols, R2-CHOH; tertiary alcohols, R3-COH. (From Grant & Hackh's Chemical Dictionary, 5th ed)

Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA. (1/999)

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4. (2/999)

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.  (+info)

Two types of HTLV-1 particles are released from MT-2 cells. (3/999)

The MT-2 cell line transformed by human T-cell leukemia virus type 1 (HTLV-1) contains one complete provirus and seven defective proviruses. Four defective genomes have an identical structure (LTR-MA-deltaCA-pX-LTR) with an open reading frame that spans from MA to pX, giving rise to a 3.4-kb (24S) RNA transcript encoding a chimeric Gag-pX protein, p28. MT-2 cells release two distinct types of virions. The major "classic" type of particle has a buoyant density of 1.155-1.16 g/cm3 and contains the standard HTLV-I structural proteins and reverse transcriptase (RT). In addition, about 5% of particles are "light," approximately 1.12 g/cm3, and contain p28, RT activity, and the 3.4-kb RNA transcript. RT-PCR and in vitro translation indicate that some of the classic HTLV-1 particles package 3.4-kb RNA as well as full-length 8.5-kb RNA. In addition to matrix features, the p28 protein has a motif resembling a zinc finger at the C-terminal, pX0 region, which may play a role in the assembly of the defective light virions.  (+info)

Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions. (4/999)

Pulmonary surfactant exists in the alveolus in several distinct subtypes that differ in their morphology, composition, and surface activity. Experiments by others have implicated a serine hydrolase in the production of the inactive small vesicular subtype of surfactant (N. J. Gross and R. M. Schultz. Biochim. Biophys. Acta 1044: 222-230, 1990). Our laboratory recently identified this enzyme in the rat as the serine carboxylesterase ES-2 [F. Barr, H. Clark, and S. Hawgood. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L404-L410, 1998]. In the present study, we determined the cellular sites of expression of ES-2 in rat lung using a digoxygenin-labeled ES-2 riboprobe. ES-2 mRNA was localized to type II cells and alveolar macrophages but not to Clara cells. Using a specific ES-2 antibody, we determined the protein distribution of ES-2 in the lung by immunohistochemistry, and it was found to be consistent with the sites of mRNA expression. Most of the ES-2 in rat bronchoalveolar lavage is in the surfactant-depleted supernatant, but ES-2 was also consistently localized to the small vesicular surfactant subfraction presumed to form as a consequence of conversion activity. These results are consistent with a role for endogenous lung ES-2 in surfactant metabolism.  (+info)

Inhibition of protein denaturation by fatty acids, bile salts and other natural substances: a new hypothesis for the mechanism of action of fish oil in rheumatic diseases. (5/999)

Natural hydrophobic substances like bile salts (cholate, deoxycholate, chenodeoxycholate, lithocholate and their conjugates with glycine and taurine), fatty acids (caprylic, capric, lauric, myristic, palmitic, stearic, oleic, linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acid) were much more active (EC50 approximately 10(-4)-10(-5) M) than selected amino acids (EC50 > 10(-2) M) and inorganic salts (EC50 approximately 10(-1) M) in inhibiting heat-induced denaturation of human serum albumin in vitro. Fish oil, rich in n-3-polyunsaturated acids such as eicosapentaenoic acid and docosahexaenoic acid, administered p.o. (1 ml/kg) in the rat, protected ex vivo (after 2 hr) serum against heat-induced denaturation more than bendazac, a known antidenaturant drug. Thus, we speculated that the antidenaturant activity of fish oil may be partly (in addition to the known effect on endogenous eicosanoid composition) responsible for its beneficial effects in rheumatoid arthritis and other rheumatic conditions. In this connection, it is of note that the in vitro antidenaturant activity of fish oil fatty acids was higher than that of known antidenaturant drugs such as bendazac and bindarit and nonsteroidal anti-inflammatory drugs like phenylbutazone and indomethacin which could exert beneficial effects in chronic inflammatory conditions by stabilizing endogenous proteins.  (+info)

European interlaboratory comparison of breath 13CO2 analysis. (6/999)

The BIOMED I programme Stable Isotopes in Gastroenterology and Nutrition (SIGN) has focused upon evaluation and standardisation of stable isotope breath tests using 13C labelled substrates. The programme dealt with comparison of 13C substrates, test meals, test conditions, analysis techniques, and calculation procedures. Analytical techniques applied for 13CO2 analysis were evaluated by taking an inventory of instrumentation, calibration protocols, and analysis procedures. Two ring tests were initiated measuring 13C abundances of carbonate materials. Evaluating the data it was found that seven different models of isotope ratio mass spectrometers (IRMS) were used by the participants applying both the dual inlet system and the continuous flow configuration. Eight different brands of certified 13C reference materials were used with a 13C abundance varying from delta 13CPDB -37.2 to +2.0/1000. CO2 was liberated from certified material by three techniques and different working standards were used varying from -47.4 to +0.4/1000 in their delta 13CPDB value. The standard deviations (SDs) found for all measurements by all participants were 0.25/1000 and 0.50/1000 for two carbonates used in the ring tests. The individual variation for the single participants varied from 0.02 /1000 (dual inlet system) to 0.14/1000 (continuous flow system). The measurement of the difference between two carbonates showed a SD of 0.33/1000 calculated for all participants. Internal precision of IRMS as indicated by the specifications of the different instrument suppliers is < 0.3/1000 for continuous flow systems. In this respect it can be concluded that all participants are working well within the instrument specifications even including sample preparation. Increased overall interlaboratory variation is therefore likely to be due to non-instrumental conditions. It is possible that consistent differences in sample handling leading to isotope fractionation are the causes for interlaboratory variation. Breath analysis does not require sample preparation. As such, interlaboratory variation will be less than observed for the carbonate samples and within the range indicated as internal precision for continuous flow instruments. From this it is concluded that pure analytical interlaboratory variation is acceptable despite the many differences in instrumentation and analytical protocols. Coordinated metabolic studies appear possible, in which different European laboratories perform 13CO2 analysis. Evaluation of compatibility of the analytical systems remains advisable, however.  (+info)

Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different from that of Salmonella LPS. (7/999)

Some lipopolysaccharide (LPS) preparations from S- or R-form members of the family Enterobacteriaceae and oral black-pigmented bacteria (Porphyromonas gingivalis and Prevotella intermedia) are known to activate LPS-refractory C3H/HeJ macrophages. When contaminating proteins are removed from R-form LPS of Enterobacteriaceae by repurification, however, this ability is lost. In the present study, we investigated the capacity of LPS from P. gingivalis, P. intermedia, Salmonella minnesota, and Salmonella abortusequi to induce production of tumor necrosis factor (TNF) in gamma interferon-primed C3H/HeJ macrophages before and after repurification. P. abortusequi S-LPS was fractionated by centrifugal partition chromatography into two LPS forms: SL-LPS, having homologous long O-polysaccharide chains, and SS-LPS having short oligosaccharide chains. Prior to repurification, all LPS forms except SL-LPS induced TNF production in both C3H/HeJ and C3H/HeN macrophages. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that repurification removed contaminating protein from the preparations, and repurified SS-LPS and S. minnesota Ra-LPS no longer stimulated TNF production in C3H/HeJ macrophages, although C3H/HeN macrophages remained responsive. In contrast, repurified oral bacterial LPS retained the capacity to induce TNF production in C3H/HeJ macrophages. Oral bacterial LPS preparations also were not antagonized by excess inactive, repurified SL-LPS; Ra-LPS; Rhodobacter sphaeroides lipid A, a competitive LPS antagonist, or paclitaxel, an LPS agonist, and they were comparatively resistant to polymyxin B treatment. Nevertheless, oral bacterial LPS was less toxic to D-galactosamine-treated C3H/HeN mice than was LPS from Salmonella. These findings indicate that the active molecule(s) and mode of action of LPS from P. gingivalis and P. intermedia are quite different from those of LPS from Salmonella.  (+info)

Characterization of a novel trypanosome lytic factor from human serum. (8/999)

Natural resistance of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in human serum of nonimmune factors that lyse the parasite. Normal human serum contains two trypanosome lytic factors (TLFs). TLF1 is a 500-kDa lipoprotein, which is reported to contain apolipoprotein A-I (apoA-I), haptoglobin-related protein (Hpr), hemoglobin, paraoxonase, and apoA-II, whereas TLF2 is a larger, poorly characterized particle. We report here a new immunoaffinity-based purification procedure for TLF2 and TLF1, as well as further characterization of the components of each purified TLF. Immunoaffinity-purified TLF1 has a specific activity 10-fold higher than that of TLF1 purified by previously described methods. Moreover, we find that TLF1 is a lipoprotein particle that contains mainly apoA-I and Hpr, trace amounts of paraoxonase, apoA-II, and haptoglobin, but no detectable hemoglobin. Characterization of TLF2 reveals that it is a 1,000-kDa protein complex containing mainly immunoglobulin M, apoA-I, and Hpr but less than 1% detectable lipid.  (+info)

This paper presents the extraction of cations from a soil sample, type ranker on serpentinite, in deionized water, by use of three different extraction techniques. The first extraction technique included the use of a rotary mixer, the second technique involved the use of a microwave digestion system with different extraction temperatures, and the third technique employed an ultrasonic bath with different extraction times. Ion chromatography was used for determining the concentration of Li, Na, K, Ca, Mg and ammonium ions in soil extracts with subsequent determination of concentrations for all cations, except for ammonium ion extraction, conducted by Inductively Coupled Plasma-Atomic Emission Spectrometry. The results of cation extractions showed that microwave assisted extraction was most efficient for the Li, Na, K, Ca, Mg, Co, Mn, Ni, Pb and ammonium ions. Use of a rotary mixer for extraction was most efficient for Cd and Zn ions, while use of ultrasound bath was most efficient for Cr, Cu, Fe ...
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Concerned about Mykaylas stomach cramps Krenzler who lives in Portland Oregon sent a sample of the oil off to Going Green Labs in Albany Oregon. Like most labs catering to the cannabis industry Going Green mainly performs THC potency tests. According to Krenzler when the lab tested his sample it found that the Real Scientific Hemp Oil contained much more THC than HempMedsPx had claimed?3.8 percent instead of roughly 1 percent. Hemp Oil Ibs krenzler said he was disturbed? by the finding and also by the implications it had for other parents of sick children.. The oil will feel dry to the touch one day after the Hemp Oil Ibs initial application however complete drying can be several days. For example if there is too much oil initially applied then small amounts of oil may rise over the first few days. This can be avoided by wiping the excess away with a cloth. If used on a floor during this time it is recommended to place some cardboard pieces where there will be a lot of foot traffic. Dirty shoes ...
ProteinSafe™ Protease Inhibitor Cocktail (100×),Protein Extraction,Protein Extraction, Purification and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionP
Description. Protein stability is fundamental to all aspects of protein research, including analysis by Western blotting. Combine our gentle protein extraction kits with protease inhibitors to obtain stabilized, intact and active proteins.. BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase®Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extraction from 20 g and 100 g cell paste. ...
of course, you can fractionize proteins by sucrose density ultracentrifugation; it works best for pre-purified fractions and macromolecular proteins f.i. oligomeric proteins or large fibrillar proteins. ...
I work on an uropathogenic strain of E.coli. When I artificially express from a plasmid one of the transcriptional regulator, it results in secretion of a protein factor in the media, which has interesting biological roles. My aim is to purify this unknown factor present in the growth media and to identify it by Mass Spec ...
Tätä artikkelia/julkaisua ei ole tallennettu Jukuriin. Julkaisun tiedoissa voi kuitenkin olla linkki toisaalle tallennettuun artikkeliin/julkaisuun. ...
Stir bars - it was the stirring bars all along. Thats the take-home from this paper, which shows definitively that (A) you cannot truly clean one of the things
Stir bars - it was the stirring bars all along. Thats the take-home from this paper, which shows definitively that (A) you cannot truly clean one of the things
SoluLyse protein extraction reagents are optimized for the most efficient extraction of soluble proteins from bacterial and mammalian cells.
RMBIO manufactures custom orders to clients in need of specialized material. With our high tech facility, we can make a diverse range of extraction. Clients typically provide the raw materials for processing, and we deliver a final product that meets specifications agreed on at the start.
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Micron-size polystyrene-based latex particles were separated by using new micro-thermal field-flow fractionation (micro-TFFF). The order of retention from the largest to the smallest particles that appears at high field strength and high flow rate corresponds to the focusing mechanism which itself is a consequence of the lift forces acting on the particles. The mechanism of steric exclusion can only be effective at low flow rates of the carrier liquid. Whenever high-speed separation was performed, the focusing effect clearly dominated the FFF mechanism. This application of micro-TFFF in focusing mode to the separation of the particles is the first one published. As a result, micro-TFFF thus became a very universal technique for the separation of synthetic and natural macromolecules and of particles of various origin and size up to large (micron-size) diameter. ,p, ...
Online Miniaturized Asymmetrical Flow Field-Flow Fractionation and Inductively Coupled Plasma Mass Spectrometry for Metalloprotein Analysis of Plasma from Patients with Lung Cancer
Volatile and semi-volatile compounds were extracted by stir bar sorptive extraction from watermelon puree and headspace obtained from 4 different varieties. Analysis was conducted by gas chromatography mass spectrometry. More than 36 compounds were detected. 10 were positively identified with authentic standard confirmation. The compound present in greatest abundance varied according to variety.. .no-margin li {margin: 0!important;} ...
Extractables and Leachables Analysis of IV Bag Systems using Direct Thermal Extraction of the Materials and Stir Bar Sorptive Extraction of Aqueous Solutions coupled with Thermal Desorption Gas-Chromatography with Unit Mass and High Resolution Mass Spectrometric ...
Stir Bar Sorptive Extraction is the primary technique for beer and wine flavor and aroma analysis. Heres a new multi-SBSE method to improve results.
In recent months the movement to embrace hemp oil as a cure-all natural? remedy that can heal without any of the toxicity of traditional treatments has begun to take hold . I did use some of the medication they hemp oil dabs prescribed but it was in combination with the oil. I am elated I want to continue sharing with people who are experiencing what I went through that there is absolutely hope and a cure out there. They just need to be proactive and aggressive with treatment not wait addition to being an effective cancer fighter there are some nice side effects that come from using the Rick Simpson oil for instance I no longer need to take any pain killers. Just one or two drops buy cannabidiol candy of the oil will ease the pain and help you sleep like a baby. Rick Simpson Oil How To Buy Best of all its natural Thanks to all the staff at Amsterdams Garden who guided me throughout this journey.. And since neither ustoms or TestPledge require CBD testing there is no incentive to test for CBD. ...
Engineered nanomaterials are subject of intense research since several years - nanoanalytical tools are mainly based on fractionation techniques such as FFF, HDC and CE coupled on-line with ICP-MS. Nowadays imaging techniques gain importance in life-science, e.g. for the application of nanomaterials or the analysis of single-cells.. Thus, the 7th Workshop on Field Flow Fractionation and Mass Spectrometry (FFF-MS) will focus on the application of nanomaterials in life science and environmental technology and the applicability of nanoanalytical tools in these fields.. A special focus will be placed on the complementary analysis of nanomaterials in life science application as well as during their synthesis. Special topics are. ...
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation. ...
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What exactly are nanoparticles and are they safe for humans and the environment? These and many more questions regarding nanoparticle safety and risks of nanotechnology are answered on nanopartikel.info.
In this study, a fully automated QuEChERS system was used to extract 204 different pesticides from various commodities. The matrices used for this evaluation were cabbage, valencia orange, raisin and purple corn flour. The performance of the Teledyne Tekmar AutoMate-Q40 automated QuEChERS platform was evaluated in comparison to a manual sample preparation workflow. Both preparations employed the AOAC 2007.01 unbuffered QuEChERS methodology, where all the samples were analyzed using liquid chromatographytandem mass spectrometry (LC-MS/MS). ...
In this study, a fully automated QuEChERS system was used to extract 204 different pesticides from various commodities. The matrices used for this evaluation were cabbage, valencia orange, raisin and purple corn flour. The performance of the Teledyne Tekmar AutoMate-Q40 automated QuEChERS platform was evaluated in comparison to a manual sample preparation workflow. Both preparations employed the AOAC 2007.01 unbuffered QuEChERS methodology, where all the samples were analyzed using liquid chromatographytandem mass spectrometry (LC-MS/MS). ...
The main goal of the work presented in this thesis is to improve the reliability of existing methods for speciation analysis of organotin compounds. Species-specific isotope dilution (SSID) calibration in combination with gas chromatography - inductively coupled plasma mass spectrometry was used to investigate the transformation of phenyltin species during sample preparation. Isotope-enriched phenyltin species were synthesized from corresponding isotope-enriched tin metals. SSID with a mixture of phenyltin species (PhTs) from one isotope was used to evaluate different extraction procedures for the determination of PhTs in fresh water sediment. Preparative liquid chromatography was used to produce single isotope-enriched phenyltin species making a multi-isotope spike (MI) SSID calibration possible. Different extraction procedures for the analysis of phenyltin species in biological samples were evaluated by applying MI-SSID. Degradation of TPhT and DPhT during sample extraction was observed and ...
... ,The ProteomeLab PF 2D is an automated, two-dimensional fractionation system for high-resolution analysis of complex protein mixtures. Part of Beckman Coulters ProteomeLab family of products, the PF 2D produces results in just hours. The system effectively resolves the thousands of proteins presen,biological,biology supply,biology supplies,biology product
Terpenes must be diluted prior to use for flavor and effect (1-5% by total volume - Ex: 2 drops per gram). Terpenes are oil soluble and mix well with plant extracts, coconut oil, and more.. Weigh then add your base extraction material to a small glass beaker.. Take (1%) of the base extraction material weight of additive (terpene or flavor) and add it to base extraction material. Use a pipette to apply the additive.. Add Magnetic Stir bar, apply to stir plate and mix for 10 minutes. Product may need a very small amount of heat if material is too thick. Heat to no more than 115 degrees F or 45 Degrees C. * If you do not have a heat plate or stir bar, use metal mixing spoon to manually mix (This option may be less evenly blended). *. Apply Mixture to your application (Rig reservoir, dab syringe, edible, beverage) and test ...
Terpenes must be diluted prior to use for flavor and effect (1-5% by total volume - Ex: 2 drops per gram). Terpenes are oil soluble and mix well with plant extracts, coconut oil, and more.. Weigh then add your base extraction material to a small glass beaker.. Take (1%) of the base extraction material weight of additive (terpene or flavor) and add it to base extraction material. Use a pipette to apply the additive.. Add Magnetic Stir bar, apply to stir plate and mix for 10 minutes. Product may need a very small amount of heat if material is too thick. Heat to no more than 115 degrees F or 45 Degrees C. * If you do not have a heat plate or stir bar, use metal mixing spoon to manually mix (This option may be less evenly blended). *. Apply Mixture to your application (Rig reservoir, dab syringe, edible, beverage) and test ...
ISOLUTE® QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) products provide simple clean-up of complex samples using salt assisted extraction and partitioning, followed by dispersive SPE.
ISOLUTE® QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) products provide simple clean-up of complex samples using salt assisted extraction and partitioning, followed by dispersive SPE.
AOAC QuEChERS IS Solution (2 components) cat.# 31963 Ready to use for generating test mixes, calibration standards, and spiking experiments.Reliable standards ...
Get this from a library! Principles and reactions of protein extraction, purification, and characterization. [Hafiz Ahmed] -- This book describes the mechanisms and experimental procedures for techniques used in protein extraction, purification, and characterization, ranging from classic to cutting-edge. Presenting the ...
To use, one simply places the requisite amount of PTS (15 wt% in water) into a test tube with a stir bar and adds the organic substrate(s) and catalyst. Reactions are generally complete within 3-24 hours and can be accelerated if needed upon mild heating to 40-50 °C. Work-up is also very simple involving either extraction of the reaction mixture with EtOAc-hexane or deposition onto a bed of silica gel and elution with EtOAc. In most examples studied, PTS outperformed other non-ionic amphiphiles such as Triton®-X100 and Brij® 30.. ...
Does anyone have suggestions for eliminating black, particulate sludge from my slides after Timms staining? Every square inch of glassware, stir bars, etc. has been acid cleaned and I am only using glass distilled water. When I add the silver nitrate to the developer I am getting a cloudy precipitate. Please advise with any and all ideas. Thanks, Nancy Lemke ...
Get a flexible, efficient cell lysis buffer for protein extraction from a wide variety of cells. This cell lysis reagent offers a 10-min lysis using mild, non-denaturing conditions.
Get a flexible, efficient cell lysis buffer for protein extraction from a wide variety of cells. This cell lysis reagent offers a 10-min lysis using mild, non-denaturing conditions.
Sage Science, a developer of products for improving sample preparation processes in life science applications, has announced the release of its new SageELF technology for DNA and protein fractionation. This sample prep instrument will be especially useful for preserving precious samples and has applications in next-generation sequencing as well as bottom-up or top-down proteomics experiments.
This protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following TRIzol isolation of RNA.
6. The hydrophobic & hydrophilic properties of the resin makes it ideal for both ion exchange and reverse phase chromatography. Norgen holds more than two dozen issued and pending patents, and has successfully exploited part of this extensive IP to develop over 150 sample preparation kits for RNA, DNA, and protein purification for research purposes. These kits are currently being sold to researchers in academia, pharma/biotech companies, and government institutions. Norgen has also demonstrated at the lab bench scale the utility of their resin technology for several industrial applications, including large scale pharmaceutical grade plasmid DNA isolation, filtration and removal of adventitious material, and large scale protein fractionation. These are all lucrative areas, and are available for out licensing. ...
Date: Apr 13, 2015. Author: separationsNOW. The precise cryogenic separation of oxygen-argon mixtures from natural air samples has been accomplished using a single 5Å (30/60 mesh) molecular sieve column at liquid nitrogen temperature, which is associated with isotopic fractionation.. Read More ...
Perform an efficient extraction of soluble proteins from bacterial cells with SoluLyse from Genlantis, a high-yielding and optimized bacterial lysis reagent.
The analysis of flavor compounds in dairy products such as milk, cream, yogurt and cheese as well as their blends with several ingredients usually requires cumbersome sample preparation steps such as liquid/liquid extraction, solid phase extraction or distillation techniques, often with the drawback of organic solvent use. Headspace and purge & trap methods do not use organic solvents, but their analyte range is restricted to volatile compounds and therefore characterize compounds that contribute to the aroma/smell of a sample, not flavor/taste. In addition heating of the sample should be avoided since this would lead to reaction products which dramatically modify the flavor and taste of any dairy product. The sensitivity of solid phase microextraction (SPME) is limited by the small amount of sorptive material that can be coated on the fibers. A new extraction technique, Stir Bar Sorptive Extraction (SBSE), that overcomes the major problems with classical extraction techniques is applied in this ...
Methods, systems and other embodiments associated with upgrading heavy oil are presented. A method (900) of upgrading heavy oil by deasphalting includes mixing (902) an initial solvent with raw heavy oil to produce a mixed heavy oil. The mixed heavy oil is heated (904) to produce heated heavy oil. High pressure pulses are created (906) in the heated heavy oil to crack the heated heavy oil to produce a cracked oil with a lower viscosity than the raw heavy oil.
This study was undertaken to optimize the conditions for the extraction of antibacterial activity of Perilla frutescens var. acuta leaf against Pseudomonas aeruginosa KCTC 2004 using the evolutionary operation-factorial (EVOP) design technique. Increased antibacterial activity was achieved at higher extraction temperatures and with a longer extraction time. Antibacterial activity was not affected by differing ethanol concentration in the extraction solvent. The maximum antibacterial activity of ethanolic extract of P. frutescens var. acuta leaf against P. aeruginosa, determined by the EVOP factorial technique, was obtained at an extraction temperature of 80 °C (R = −0.800**), 26 h (R = −0.731**) extraction time, and 50% (R = −0.075) ethanol concentration. The population of P. aeruginosa also decreased from 6.660 log CFU/mL in the initial set to 4.060 log CFU/mL in the third set. Also, scanning electron microscopy study of the ethanolic extract of P. frutescens var. acuta revealed potential
Global Heavy Oil Hydrotreating Catalyst Market 2017, presents a professional and in-depth study on the current state of the Heavy Oil Hydrotreating Catalyst market globally, providing basic overview of Heavy Oil Hydrotreating Catalyst market including Definitions, Classifications, Applications and Industry chain structure, Heavy Oil Hydrotreating Catalyst Market report provides development policies and plans are discussed as well as manufacturing processes and cost structures. Heavy Oil Hydrotreating Catalyst market size, share and end users are analyzed as well as segment markets by types, applications and companies.. Download Free Sample Report @ http://www.fiormarkets.com/report-detail/34066/request-sample. Global market research report of Heavy Oil Hydrotreating Catalyst 2017 mainly focuses on Production, means the output of Heavy Oil Hydrotreating Catalyst and Revenue, means the sales value of Heavy Oil Hydrotreating Catalyst in market ...
Abstract: Microwave-assisted extraction was investigated for extraction of polysaccharides from Catathelasma ventricosum using Response Surface Methodology (RSM). The effects of operating conditions (extraction time, microwave power and ratio of water to raw material) on the extraction yield of polysaccharides were studied. A Box-Behnken Design was used for experimental design and analysis of the results to obtain the optimal processing parameters. Among the three extraction parameters, the microwave power was the most significant factor to affect the yield of polysaccharides from Catathelasma ventricosum. In addition, microwave power and extraction time had significant interaction effects. A mathematical model with high determination coefficient was gained and could be employed to optimize polysaccharides extraction and the optimal conditions were extraction time 5 min, microwave power 420 W, ratio of water to raw material 25:1. Under these conditions, the experimental yield of polysaccharide ...
Macrophage suppression has been shown to be mediated by a unique, low molecular weight fraction of murine serum. The present investigation involves the in vitro production of this macrophage modulator (suppressor) by Concanavalin A-stimulated spleen cells. Spleen cell culture supernatant containing macrophage suppressor factor (MSF) caused a significant decrease in in vitro phagocytosis of Listeria monocytogenes by non-elicited peritoneal macrophages. The molecular weight of MSF was determined by ultrafiltration to be less than 10,000, and the modulating activity of MSF was not altered by heating at 100°C for 30 minutes or freezing at -70°C for six months. MSF is resistant to treatment with Pronase E, but is, however, sensitive to acid hydrolysis. Activity of MSF in spleen cell culture supernatants from normal mice does not differ from supernatants from mice immunized with L. monocytogenes. It was therefore concluded that MSF is not affected by antigenic stimulation and is apparently produced
View kits, reagents and equipment for quick protein fractionation by physical properties or cellular location. Improve identification of low-abundance proteins.
Liposomes are currently being investigated as potential parenterally used drug carriers. The main factor that influences the in vivo behavior of such liposomes is their vesicle size. A detailed and reliable knowledge of vesicle size is therefore necessary in order to interpret results of physical and biological investigations in a correct manner. It has earlier been discovered that it is feasible to determine the size distribution of vesicle dispersions in a reliable manner and it appears especially useful to employ the combination of SEC fractionation, PCS and the enzymatic PC quantitation. A drawback discovered was for certain liposome dispersions that in some of the dispersions SEC fractionation showed incomplete recovery of the vesicles. This indicated a demand for a different fractionation method which does not have the limitations that the SEC method described above has. To this end flow field-flow fractionation was chosen. In a previous study the influence of some key factors such as ...
Theoretical Study on the Extraction of Alkaline Earth Salts by 18-Crown-6: Roles of Counterions, Solvent Types and Extraction Temperatures
The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP). For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%). For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%). On the ...
GE Healthcare Yeast Protein Extraction Buffer Kit Yeast protein extraction buffer kit Life Sciences:Protein Biology:Protein Extraction and Purification:Protein Extraction:Cell
Postnova Analytics reports on exciting advances in the analysis of plasma proteins using a hyphenated system that couples asymmetric flow Field-Flow Fractionation (AF4) and inductively coupled plasma-mass spectrometry (ICP-MS).  In a new application study researchers show how an AF4-ICP-MS system is able to obtain valuable information on the multi-element speciation and absolute molecular mass of human plasma proteins such as Albumin and Transferrin. Human Serum Albumin (HSA) and Transferrin are the two main plasma proteins that are known to bind and transfer metal ions in biological fluids. Metal-protein complexes are involved...
misc{8892516, abstract = {The physicochemical properties of oat β-glucan have been shown to govern its health benefits by a plethora of studies. However, the effect of the passage through the gastrointestinal tract on the conformational and structural characteristics is not fully explained. The aim of the present study was to elucidate the effect of in vitro gastrointestinal conditions on the structure and conformation of oat β-glucan utilizing Asymmetric Flow Field-Flow Fractionation (AF4) coupled with multiple detectors and in conjunction with NMR. Oat β-glucan was gently extracted from oat flour and its conformational characteristics before and after in vitro gastric and gastrointestinal digestion was investigated with AF4. The contribution of digestive enzymes and bile acids on the conformation of β-glucan was also explored. Moreover, the structural features and the bile acid-binding capacity of oat β-glucan were analyzed with NMR spectroscopy. Oat β-glucan without digestion presented ...
article{fb76340e-5768-4989-8c20-3ad5b72c0e89, abstract = {,p,A plethora of studies have shown that the physicochemical properties of oat β-glucan determine its health benefits. However, the impact of the passage through the gastrointestinal tract on the conformational and structural characteristics is not fully understood. The present study aims to elucidate the structure and conformation of gently extracted oat β-glucan before and after in vitro gastric and gastrointestinal digestion utilizing asymmetric flow field-flow fractionation (AF4) and NMR spectroscopy. The structural features and the bile acid-binding capacity of oat β-glucan were probed with NMR. Oat β-glucan without digestion presented primary aggregates with fringed micelle structure, and other high molar mass supramolecular secondary aggregates were detected. Under gastric conditions, the molar mass was reduced and an increase in apparent density, suggesting more compact and disrupted aggregates, was observed. In the intestinal ...
Thermo Scientific™ HyperSep™ Dispersive SPE Extraction Products (QuEChERS) 6g anhydrous MgSO|sub|4|/sub|, 1.5g NaCl, 1.5g sodium citrate tribasic...
Toxicological analysis of bone During a post-mortem examination, determining whether the deceased has ingested a drug and if so, when and how much can be critical to establishing the cause of death. Usually, this involves analysis of blood, urine, hair, and perhaps tissue samples from the liver or kidneys. In circumstances where the body has lain...
A biomarker for RA was identified in the synovial fluid of patients with RA using a modified strategy previously used to identify cancer biomarkers. An Agilent immunodepletion column was used to remove six abundant serum proteins (albumin, antitrypsin, haptoglobin, IgA, IgG, and transferrin) then the depleted synovial fluid was fractionated using a Beckman ProteomeLab PF2D Protein Fractionation System. Proteins were fractionated in the first dimension by chromatofocussing and in the second dimension by reverse phase High Performance Liquid Chromatography. This protein fractionation strategy increased the chance of identifying the lower abundance proteins by separating them from the higher abundance proteins, which could mask their presence. The second dimension fractions were used to construct protein arrays on nitrocellulose membranes, which were used to test for the presence of autoantigens by analysing differential reactivity of RA and control sera. Only sera obtained from RA patients is ...
There is limited experience and methods for extractions of drug therapy data from electronic health records (EHR) in the hospital setting. We have therefore developed and evaluated completeness and consistency of an automatic versus a semi-automatic extraction procedure applied on prescribing and administration of the TNF inhibitor infliximab using a hospital EHR system in Karolinska University Hospital, Sweden. Using two different extraction methods (automatic and semi-automatic), all administered infusions of infliximab between 2007 and 2010 were extracted from a database linked to the EHR system. Extracted data included encrypted personal identity number (PIN), date of birth, sex, time of prescription/administration, healthcare units, prescribed/administered dose and time of admission/discharge. The primary diagnosis (ICD-10) for the treatment with infliximab was extracted by linking infliximab infusions to their corresponding treatment episode. A total of 13,590 infusions of infliximab were ...
EDGE Application Note Abstract The QuEChERS method has been shown to be practical for pesticide analysis on a number of different sample types and is increasingly being employed on more difficult matrices.  Unfortunately, this process is a manual multi-step process that can be time consuming. With so many different types of food matrices and pesticide residues to be analyzed there is a need for a faster simplified extraction method. Furthermore, some matrices either by their nature or their economic value, can be difficult to analyze with just the QuEChERS method alone. In this application note we propose the EDGE™ as an alternative to the QuEChERS method for the extraction of pesticides from food matrices including known difficult matrices. With its patent pending Q-Cup Technology™ the EDGE can extract a difficult food sample, including the dispersive solid phase cleanup, in less than 5 minutes in one automated
Purchase Introduction to Enhanced Recovery Methods for Heavy Oil and Tar Sands - 2nd Edition. Print Book & E-Book. ISBN 9780128499061, 9780128018750
Stable and radioactive isotopes are extensively used as tracers of numerous processes in the planetary and terrestrial environment. The relative abundances of isotopic species measured by their ratios
Remove from microwave and let cool by either sitting on bench top or adding stir bar and placing on stir plate. ,br, The advantage of the stir plate is that, if you forget about your gel for a while, it is less likely to solidify accidentally. ,br, If you are in a hurry, you can place the bottle in a beaker of room temperature water on the stir plate to speed the cooling process significantly ...
The existing conceptions on the problem of liquid phase sintering fail to give answers to many questions like the following: why do compacts during liquid-phase sintering grow at first and then...
Stir 2-3 hours (not overnight) in a 2.8 liter Fernbach flask and large magnetic stir bar. Do not heat. Do not store overnight in presence of resin. Residual catalyst from the resin will polymerize the acrylamide. Filter through a Buchner funnel using Whatman #3 over Whatman #1 into a 4 Liter sidearm flask or use a 0.45 micron disposable filter. Rinse resin. Adjust volume to 1.9 liters. Immediately rinse MB3 resin from Fernbach flask as dry resin will stick very firmly ...
HPLC Training Course March 6, 2018 at 8:00 am - 5:00 pm London, UK Register Here: http://ow.ly/mwTX30igJEpLearn how to set up and run HPLC analysis with full understanding of all the method parameters such as the column, the mobile phase, the instrumentation, and sample preparation, and how to interpret and quantify the results of the analysis. ...
HPLC Training Course March 6, 2018 at 8:00 am - 5:00 pm London, UK Register Here: http://ow.ly/mwTX30igJEpLearn how to set up and run HPLC analysis with full understanding of all the method parameters such as the column, the mobile phase, the instrumentation, and sample preparation, and how to interpret and quantify the results of the analysis. ...
Date: Jan 27, 2014. Author: spectroscopyNOW. The different aspects and methodologies related to microwave-assisted extraction of botanical materials that were subjected to experimental design have been reviewed, along with some general chemometric tools and the steps involved in its practice.. Read More ...
Buy Principles and Reactions of Protein Extraction, Purification, and Characterization (9780849320347): NHBS - H Ahmed, CRC Press
Pulsatron Pump LPE4MA-VHC1-500 - E Series Chemical Metering Pumps from Pulsafeeder are in Stock at www.PumpCatalog.com - Click to Order!
A new sensitive, selective, fast and accurate technique for online sample preparation was developed. Microextraction in a packed syringe (MEPS) is a new miniaturised, solid-phase extraction (SPE) technique that can be connected online to GC or LC without any modifications. In MEPS approximately 1 mg of the solid packing material is inserted into a syringe (100-250 ml) as a plug. Sample preparation takes place on the packed bed. The bed can be coated to provide selective and suitable sampling conditions. The new method is very promising. It is very easy to use, fully automated, of low cost and rapid in comparison with previously used methods. The determination of polycyclic hydrocarbons (PAHs) in water was performed using MEPS as sample preparation method online with gas chromatography and mass spectrometry (MEPS-GC-MS). The results from MEPS as sample preparation were compared with other techniques such as stir bar sorptive extraction (SBSE) and solid-phase microextraction (SPME). The method was ...
The presence of asphaltenes in heavy oil is related to its high viscosity which is a major constraint in heavy oil processing and transportation. Moreover, problems associated with the deposition of asphaltenes at different stages of the heavy oil refining steps increase the cost of heavy oil processing. In this research project, we have achieved viscosity reduction of heavy oil by treating it through the novel JetShear destructuring process. Subsequently we have studied the adsorption of the asphaltenes over specific solid adsorbents. Characterization of the raw and treated heavy oil has been conducted. We have experimentally shown that the JetShear destructuring process reduces diluent requirements (up to 50%), decreases the initial viscosity of heavy oil and lowers the oil density (increasing its API degree) thus providing a solution for pipeline transportation.The asphaltenes content of the treated product oil was also found to decrease slightly during the JetShear destructuring as per SARA ...
In this paper, a new method of annular platform-supported headspace liquid-phase microextraction (LPME) was designed using ionic liquid as an extraction solvent, wherein extraction stability and efficiency were improved by adding an annular platform inside the extraction bottle. The ionic liquid 1-silicyl-3-benzylimidazolehexafluorophosphate was first synthesized and proved to be an excellent extraction solvent. Coupled with liquid chromatography, the proposed method was employed to analysis of polycyclic aromatic hydrocarbons (PAHs) in water and optimized in aspects of extraction temperature, extraction solvent volume, extraction time, pH, stirring rate, and salt effect of solution. The results indicated that this method showed good linearity (|i|R|/i||sup|2|/sup| > 0.995) within 0.5 |i|µ|/i|g·L|sup|−1|/sup| to 1000 |i|µ|/i|g·L|sup|−1|/sup| for PAHs. The method was more suitable for extraction of volatile PAHs, with recoveries from 65.0
A new method based on accelerated solvent extraction (ASE) combined with response surface methodology (RSM) modeling and optimization has been developed for the extraction of four lignans in Fructus Schisandrae (the fruits of Schisandra chinensis Baill). The RSM method, based on a three level and three variable Box-Behnken design (BBD), was employed to obtain the optimal combination of extraction condition. In brief, the lignans schizandrin, schisandrol B, deoxyschizandrin and schisandrin B were optimally extracted with 87% ethanol as extraction solvent, extraction temperature of 160 °C, static extraction time of 10 min, extraction pressure of 1,500 psi, flush volume of 60% and one extraction cycle. The 3D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. Under the above conditions, the experimental value of four lignans was 14.72 mg/g, which is in close agreement with the value predicted by the model.
This paper describes a laboratory study of potential post-cold production strategies for heavy oil reservoirs. The tests were conducted on two primarily depleted long core systems (glassbeads core and sandpack core). The glassbeads core is 18.2 m in length with higher permeability (11.8 Darcy) and the sandpack core is 18.5 m long with lower permeability (1.9 Darcy). Both core systems were recharged by CH4 injection followed by a depletion step similar to the cold production experiment. Both cores were then exposed to a single cycle of CO2 injection, soaking and production. This work aims at further understanding the heavy oil solution gas drive mechanism. Furthermore, the study aims at assessing methane and carbon dioxide recharging as a potential recovery method for heavy oil reservoirs, and at establishing a base line for comparison against each other. Results of this study indicate that CH4 and CO2 recharge processes give an additional 8% and 9% OOIP recovery for the glassbeads core and the ...
0109] Into a 600 ml glass beaker with a magnetic stir bar was introduced 500 ml of high purity (HP) water. A 1.605 gm quantity of DL-arginine was dissolved in this volume and the solution was heated to about 40° C., thereby forming an alkaline solution. An aqueous solution containing a 10.0 gm quantity of Ce(NO3)36(H2O) was added under high shear mixing conditions provided by a Silverson L4RT high shear mixer operated at about 5000 RPM, resulting in a pale orange colored solution of about pH 7.9. The molar ratio of DL-arginine to cerium ion was 0.4. Then a 10 ml solution containing 1.2 gm of 50% H2O2 (0.75 molar ratio of H2O2 to cerium ion) was added slowly to the cerium and arginine solution mixture, which lowered the pH to about 3.4, and formed a turbid dark brown/orange suspension. The pH was further adjusted to about 2 by the addition of 1N HNO3, thereby forming a clear orange liquid. The reaction product was then stirred with a magnetic stir bar for 1 hour at 40° C. After cooling ...
A fast and simple modified QuEChERS extraction method was developed for determination of Benzo[a]pyrene (BaP) in 137 traditional (Sangak), semi-industrial (Sangak) and industrial bread samples using spiked calibration curves by GC/MS. Sample preparation includes extraction of BaP into acetone followed by cleanup with dispersive solid phase extraction. The limit of detection and limit of quantification were 0.3 ng/g and 0.5 ng/g, respectively. The values for recoveries and RSD were calculated as 110.5-119.85% and ,11.6% respectively. Average concentration of BaP in Sangak bread samples of Shiraz and Tehran was 0.59 and 0.60 ng/g, respectively. 35.5% of samples of breads collected in Tehran were contaminated with BaP at the amount higher than maximum levels regulated in processed cereal-based foods and baby foods by European Commission (1 ng/g). Seventeen percent of samples of breads collected in Shiraz were contaminated with BaP which 13 % of total samples were ,1 ng/g. BaP content in all ...
• NEW Dual Channel System |br/| • Uses ‘amiNO’ sensors which are up to 100 times more sensitive than others on the market today |br/| &bul
Heavy Oil Processing and Extraction - Download as PDF File (.pdf), Text File (.txt) or view presentation slides online. Heavy Oil Extraction and Processing
Citation: Lehotay, S.J., Son, K.A., Kwon, H., Koesukwiwat, U., Fu, W., Mastovska, K., Hoh, E. 2010. Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A. 1217:2548-2560. Interpretive Summary: Pesticide residue analysis must be done for a variety of reasons, and the most effective and efficient analytical method should be used to achieve the best results in the fastest and most cost-effective manner. The "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) approach to pesticide residue analysis meets the needs of the application, but the concept is so flexible that three different versions are commonly used by different laboratories. This paper describes a study that is designed to answer the question, "Which version of QuEChERS is best?" The results show that all three QuEChERS methods perform very well, but AOAC Official Method 2007.01 was found to give better overall results. It is also somewhat ...
The dispersive cleaning kit for the SiliaQuick™ QuEChERS, for the EN 15662 method, used for general fruits & vegetables. In a 15mL format.
Albumin is one of the most extensively used proteins in biological research today. It acts as a powerful antioxidant in cell culture. It binds, sequesters and stabilizes a variety of molecular species which are often unstable. This acidic, soluble protein has both high-affinity and secondary binding sites, optimizing the roles that fatty acids, metals, disulfides, and other molecules play in cellular metabolism. Seralab BSA products are prepared by heat shock with the exception of GEM-700-108 which is produced by a modified method of the Cohn cold ethanol fractionation method. Heat shock produces the highest purity BSA product Cohn fractionation method remains the gold standard - this method preserves the biological activity of albumin. Ready-to-buy solutions offer the convenience of not having to solubilise BSA in - house. Available in 20g, 100g and 1kg quantities (lyophilised); or 50mL, 100mL and 1L volumes (solution). Sourced serum or plasma is filtered through a 0.2 μm filter before
Giana Lampreda is Co-Founder/Owner and Chief Officer of Operations for WAM Oil, a Seattle-based company founded in 2011, specializing in SFE Co2 cannabis oil extractions; a company that is for the people and stands by the people.. Giana, a Seattle University graduate, an educator and cannabis oil manufacturer recognized the healing effects of cannabis early on in her childhood life when she woke up with debilitating pain that was later diagnosed as cancer. As a patient first, she understood the importance of producing safe cannabis oil for consumption. In conjunction with her team; Giana began to work with different extraction methodologies to help bring products, manufactured with the safety of the public in mind, to the market.. Giana is an advocate that has spoken locally and internationally on the safe production and consumption of all cannabis products. Giana and her team continue to perfect simple Co2 extraction methods in order to produce clean, pure and healthy oil concentrates that are ...
Using our model for the simulation of photoemission of high brightness electron beams, we investigate the virtual cathode physics and the limits to spatio-temporal and spectroscopic resolution originating from the image charge on the surface and from the profile of the exciting laser pulse. By contrasting the effect of varying surface properties (leading to expanding or pinned image charge), laser profiles (Gaussian, uniform, and elliptical), and aspect ratios (pancake- and cigar-like) under different extraction field strengths and numbers of generated electrons, we quantify the effect of these experimental parameters on macroscopic pulse properties such as emittance, brightness (4D and 6D), coherence length, and energy spread. Based on our results, we outline optimal conditions of pulse generation for ultrafast electron microscope systems that take into account constraints on the number of generated electrons and on the required time resolution. ...
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Situated 70 miles (110 kilometers) offshore in water depths of approximately 3,900 feet (1,190 meters), Papa Terra is a heavy oil subsea development located within Block BC-20 of the southern Campos basin. The project will feature the first tension leg well platform in Brazil which will be connected to a floating production, storage, and offloading vessel. The completed facility will be capable of producing up to 140,000 barrels of crude oil per day ...
Rafael: Thank you very much for your very nice blog..and for all of the very nice articles you write in sample prep! I would like to add that the marriage of stir bar dispersive liquid microextraction and magnetic ionic liquids came about after I heard Prof. Alberto Chisverts talk at Extech 2016 in Poland. At the conclusion of his talk, he mentioned his interest in pursuing other magnetic sorbents, such as magnetic ionic liquids. At that time, my group had been working on optimizing the synthesis of hydrophobic magnetic ionic liquids and their use in analytical separations. Upon leaving Torun, a collaboration was born where Alberto and Dr. Juan Luis Benedé used our MILs to develop a very elegant extraction method. This is a great example of how attending conferences allows for open dialog of ideas and the creation of new ideas/collaborations! ...
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An arrangement for chemical analysis of a small quantity of sample wherein a specimen of a small size is passed through a porous distribution first medium onto a reagent-containing second medium is disclosed. The reagent-containing second medium is a thin, flat, liquid-impervious medium. A reagent is encapsulated upon the second medium as a flat, liquid-phase surface. The first and second mediums are so arranged and disposed that when firmly pressed together, the encapsulated liquid reagent will be liberated and the specimen will be distributed through the first medium onto the liquid-phase liberated reagent where the subsequent reaction of the liquid-phase reagent and the specimen can then be identified by reading means.
The Presto™ DNA RNA Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA and total protein simultaneously from cultured cells, animal tissue, whole blood and biological fluids such as serum and plasma.
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Hello, I would like to isolate protein from cultured cells to perform Western blot. What is the best method for extraction, to lyse the cells by adding Triton X-100 to the buffer or just leaving Triton X-100 out the extraction buffer and completing 4 or 5 freeze-thaw cycles? Thank you, Ruben ...
Thus, I was secretly pleased that I am eligible and the actual blood extraction time was merely 5 minutes! Ultimately, my blood might not be enough to save any live (as no one can control the outcome) but it sure will increase the chance of survival for the needy. Ive pledged myself to be a regular blood donor from now onwards (at least twice a year), hence, my first blood but never my last! ...
[123 Pages Report] Check for Discount on Global and Europe Plasma Fractionation Market - Analysis and Outlook to 2022 report by QYResearch Group. This report presents a comprehensive overview of the Plasma Fractionation...
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I am trying to carry out the fractionation of cardiac tissue.I am specifically looking to isolate mitochondria, myofibril and nuclei. Anyone got any ideas ...
Author for correspondence: Email: [email protected] Shubashini K. Sripathi, Poongothai G./ Bioassay- Guided Fractionation… Table 1 Anti-fungal screening result (Zone of inhibition in mm)Sample Concentrated pet-etherresidueConcentrated Table 2 Anti-fungal screening result for column fractions and for isolated compounds against Monascus purpureus Table 2 shows anti-fungal screening result for column Alloxan-induced diabetic rats. International journal fractions against Monascus purpureus and column of Applied Research in natural products. 2009; fractions 4, 5,6,7,8 and 11 showed a higher zone of inhibition than the standard Clotrimazole. The Minimum Radha R, Arokiyaraj S, Agastian P, Balaraju K, Inhibitory Concentration (MIC) values of these column Mohan Kumar R, Bula P. Phytochemical analysis fractions were also shown in Table 2. This study also and anti-inflammatory activity of Pisonia grandis revealed the potentially active fractions from which the R.Br. Journal of Biomedical and Pharmacology ...
0049]The diesel boiling range distillate and hydrogen in line 44 is fed once-through to the liquid-phase continuous reaction zone 16 and to first liquid-phase continuous reactor 52 and/or second liquid-phase continuous reactor 56. No effluent from the liquid-phase reaction zone 16 which may comprise ultra low sulfur diesel is recycled back to the reaction zone 16. No effluent from the first liquid-phase continuous reactor 52 which may comprise ultra low sulfur diesel is recycled back to the first liquid-phase continuous reactor 52. No effluent from the second liquid-phase continuous reactor 56 which may comprise ultra low sulfur diesel is recycled back to the second liquid-phase continuous reactor 56. The diesel boiling range distillate fed to the first liquid-phase reactor 52 is absent liquid-phase reaction zone diesel effluent and/or hydrogen recycled from the first liquid-phase reactor 52. The diesel boiling range distillate fed to the second liquid-phase reactor 56 is absent liquid-phase ...
May 15, 2017). "A molecular zoom into soil Humeome by a direct sequential chemical fractionation of soi". The Science of the ... A sequential chemical fractionation called Humeomics can be used to isolate more homogeneous humic fractions and determine ... However, single classes of residual biomolecules have been identified by selective extractions and chemical fractionation, and ... 2 Chemical characteristics of humic substances in nature. *3 Chemical characteristics of traditionally produced humic ...
When there is not equilibrium between phases or chemical compounds, kinetic fractionation can occur. For example, at interfaces ... Biological fractionation is a form of kinetic fractionation, since reactions tend to be in one direction. Biological organisms ... Kinetic fractionation generally is enhanced compared to equilibrium fractionation, and depends on factors such as reaction rate ... Equilibrium fractionation occurs between chemicals or phases that are in equilibrium with each other. In equilibrium ...
The types of synchronizations are broadly categorized into two groups: "Physical Fractionation" and "Chemical Blockade." ... Physical fractionation or cell separation techniques, based on the following characteristics are in use. Cell density Cell size ...
XXIV.-On chemical analysis by spectrum-observations. Quarterly Journal of the Chemical Society of London. 1861, 13 (3): 270. ... Lithium isotopic signatures of peridotite xenoliths and isotopic fractionation at high temperature between olivine and ... Chemical Geology. 2004, 212 (1-2): 163-177. Bibcode:2004ChGeo.212..163S. doi:10.1016/j.chemgeo.2004.08.009.. ... Krebs, Robert E. The history and use of our earth's chemical elements: a reference guide. Greenwood Publishing Group. 2006: 47 ...
"XXIV.-On chemical analysis by spectrum-observations". Quarterly Journal of the Chemical Society of London. 13 (3): 270. 1861. ... "Lithium isotopic signatures of peridotite xenoliths and isotopic fractionation at high temperature between olivine and ... "IHS Chemicals. February 2012.. *^ Yurkovetskii, A. V.; Kofman, V. L.; Makovetskii, K. L. (2005). "Polymerization of 1,2- ... Krebs, Robert E. (2006). The history and use of our earth's chemical elements: a reference guide. Greenwood Publishing Group. ...
"XXIV.-On chemical analysis by spectrum-observations". Quarterly Journal of the Chemical Society of London. 13 (3): 270. 1861. ... "Lithium isotopic signatures of peridotite xenoliths and isotopic fractionation at high temperature between olivine and ... Krebs, Robert E. (2006). The history and use of our earth's chemical elements: a reference guide. Greenwood Publishing Group. s ... juli 2007). Encyclopedia of Chemical Processing and Design: Volume 28 - Lactic Acid to Magnesium Supply-Demand Relationships. M ...
Integrating bioassay-directed fractionation with chemical analysis, the team identified the toxin on the afternoon of December ... and Pseudo-nitzschia links at the ISSHA website Domoic acid at International Programme on Chemical Safety DOMOIC ACID - A MAJOR ...
Reprinted in: Gilpin and Cram (1908) "The fractionation of crude petroleum by capillary diffusion," American Chemical Journal, ... Runge dropped spots of different inorganic chemicals onto circles of filter paper already impregnated with another chemical, ... Tsvet applied his observations with filter paper extraction to the new methods of column fractionation that had been developed ... Sample images for friends of beauty and for use by sketchers, painters, decorators, and printers, prepared by chemical ...
Utilizing the then new techniques of ultra-centrifugation and chemical fractionation, Smadel made significant contributions to ...
... various ChIP-seq and other sequencing methods combined with proteomic fractionations, and sequencing methods that find chemical ... Metabolomics: Scientific study of chemical processes involving metabolites. It is a "systematic study of the unique chemical ...
... foam fractionation, chemical flocculation, and constructed wetlands. But because of the impracticality and / or high costs of ...
Crystallization elution fractionation (CEF): high-throughput chemical composition distribution analyzer by CEF TGIC: technique ... Chemical composition distribution Chemical composition distribution (CCD) together with the molar mass distribution (MMD) and ... crystallization analysis fractionation). TREF: performs the temperature rising elution fractionation (TREF) technique for the ... Preparative fractionation PREP mc2: preparative instrument to fractionate polymers by molar mass or composition (TREF or ...
"Advanced fractionation technology for the oleochemical industry". Asia-Pacific Journal of Chemical Engineering. 2: 315. doi: ...
... postulates that certain isotopic compositions of chemical elements affect kinetics of chemical reactions involving molecules ... 4. Stable nuclide Mass independent isotope fractionation Heavy water R.A. Zubarev et al., Early life relict feature in peptide ... As an example of isotopic symmetry (in compositional, and not in geometrical sense) affecting the kinetics of physic-chemical ... processes, see mass independent isotope fractionation in ozone O3. According to the IsoRes hypothesis, there are certain ...
"XXIV.-On chemical analysis by spectrum-observations". Quarterly Journal of the Chemical Society of London. 13 (3): 270. 1861. ... Nuclear weapons manufacture and other nuclear physics applications are a major source of artificial lithium fractionation, with ... stone') is a chemical element with symbol Li and atomic number 3. It is a soft, silvery-white alkali metal. Under standard ... Chemical resemblances between the two metals include the formation of a nitride by reaction with N2, the formation of an oxide ...
University of Leeds Chemical plant Foam fractionation Industrial process Process (engineering). ... a chemical process is a method or means of somehow changing one or more chemicals or chemical compounds. Such a chemical ... More than one chemical plant may use the same chemical process, each plant perhaps at differently scaled capacities. Chemical ... In addition to chemical plants for producing chemicals, chemical processes with similar technology and equipment are also used ...
... compositions of any meteorites and are often used as a standard for assessing the degree of chemical fractionation experienced ... "Primitive," in this sense, means that the abundances of most chemical elements do not differ greatly from those that are ... Van Schmus, W. R.; Wood, J. A. (1967). "A chemical-petrologic classification for the chondritic meteorites". Geochimica et ... A chondrite's group is determined by its primary chemical, mineralogical, and isotopic characteristics (above). The degree to ...
However, single classes of residual biomolecules have been identified by selective extractions and chemical fractionation, and ... Journal of the Chemical Society, Chemical Communications (23): 1565-6. doi:10.1039/C39840001565. Synthesis of fulvic acid (1a) ... Humic and fulvic acids, when present in treated drinking water, can react with the chemicals used in the chlorination process ... doi:10.1016/S0065-2113(02)75003-7. ISBN 978-0-12-000793-6. Tipping, E (1994). "'WHAM - a chemical equilibrium model and ...
The relative abundances of different isotopes are then used to describe the chemical fractionation of different isotopes, ... Variants of this technique are ID-TIMS (ID = Isotope Dilution) and CA-TIMS (CA = Chemical Abrasion). ...
... may refer to: Magnetized Inertial Fusion, a method of generating energy Mass-independent fractionation, any chemical or ...
... active chemical migration and fractionation, and low-viscosity. They further write, "These features lead to a concept of dual ...
... metamorphism in the early solar nebula-evaporation experiments on the melt FeO-MgO-SiO2-CaO-Al2O3 and chemical fractionations ... "Chemical and mineralogical compositions of cosmic and terrestrial spherules from a marine sediment", Journal of Geophysical ... mineralogy and chemical composition", Geochimica et Cosmochimica Acta, 53 (4): 937-944, Bibcode:1989GeCoA..53..937K, doi: ... mineralogy and chemical composition", Geochimica et Cosmochimica Acta, 53 (4): 937-944, Bibcode:1989GeCoA..53..937K, doi: ...
The chemical species of interest can be represented via the reaction: 2CH2O + SO42− → H2S + 2HCO3− In the reaction above the ... Sørensen, Ketil B; Canfield, Donald E (2004-02-01). "Annual fluctuations in sulfur isotope fractionation in the water column of ... Nägler, T. F.; Neubert, N.; Böttcher, M. E.; Dellwig, O.; Schnetger, B. (2011-10-07). "Molybdenum isotope fractionation in ... Chemical Geology. 206: 347-372. Bond, David; Wignall, Paul B.; Racki, Grzegorz (2004-03-01). "Extent and duration of marine ...
NGL stream is instead transported as a mixed product to standalone fractionation complexes located near refineries or chemical ... The recovered NGL stream is sometimes processed through a fractionation train consisting of three distillation towers in series ... Currently, most cryogenic plants do not include fractionation for economic reasons, and the ... and providing hot water Fuel for environmentally friendly compressed or liquid natural gas vehicles Raw material for chemical ...
... chemical kinetics, fractionation, and pressure drop. HYSYS is used extensively in industry and academia for steady-state and ... is a chemical process simulator used to mathematically model chemical processes, from unit operations to full chemical plants ... HYSYS is able to perform many of the core calculations of chemical engineering, including those concerned with mass balance, ... Schaschke, Carl (2014). A Dictionary of Chemical Engineering Oxford Quick Reference. OUP Oxford. p. 191. ISBN 9780191002700. ...
Fractionation processEdit. General method of fractionation: Cell sample is stored in a suspension which is: *Buffered - neutral ... Current Opinion in Chemical Biology, Vol. 10, 2006. pp. 430-436.. *Dam, J., Velikovsky, C.A., Mariuzza R.A., et al. ... In the chemical and food industries, special centrifuges can process a continuous stream of particle-laden liquid. ... Ultracentrifuges can also be used in the study of membrane fractionation. This occurs because ultracentrifuges can reach ...
... a sequential Sr fractionation scheme was developed for wheat flour matrix. The lipid, water-soluble, and starch fractions were ... A Novel and Validated Chemical-Enzymatic Strontium Fractionation Method for Wheat Flour from Celestite Mining Area: the First ... Erdemir US, Gucer S (2016a) Characterization of copper bioavailability in wheat flour by chemical fractionation and inductively ... Guidelines for terms related to chemical speciation and fractionation of elements. Definitions, structural aspects, and ...
The Canadian Journal of Chemical Engineering. Volume 57, Issue 3, pages 321-326, June 1979 ... Zajic, J. E., Berk, D. and Behie, L. A. (1979), Foam fractionation of spent sulphite liquor: Part I: Separation of surfactants ...
Contact allergy to oak moss: search for sensitizing molecules using combined bioassay-guided chemical fractionation, GC-MS, and ... The method is based on the combination of bioassay-guided chemical fractionation, gas chromatography-mass spectrometry analysis ... Identification of Lilial as a fragrance sensitizer in a perfume by bioassay-guided chemical fractionation and structure- ... In: Smith CK, Hotchkiss SAM (eds) Allergic contact dermatitis: chemical and metabolic mechanisms. Taylor & Francis, London, pp ...
... driven chemical fractionation. This research isolated and identified compounds in heavy fuel oil 7102 (HFO 7102) that are ... driven chemical fractionation combined the chemical separation of oil with toxicity testing and chemical analyses of each ... driven chemical fractionation. Be the first to comment! ... a high energy chemical dispersion of oil in water, which ... Identification of compounds in heavy fuel oil that are chronically toxic to rainbow trout embryos by effects‐driven chemical ...
Fractionation of PDAG fat changes the chemical composition of liquid and solid fractions. In terms of FAC, the major fatty acid ... Studies showed that the properties of fractionated end products can be affected by fractionation processing conditions. In the ... present study, dry fractionation of palm-based diacylglycerol (PDAG) was performed at different: cooling rates (0.05, 0.5, 1.0 ... Fractionation which separates the olein (liquid) and stearin (solid) fractions of oil is used to modify the physicochemical ...
Ramachandran M. Chemical fractionation of Brucella SPP. Indian Journal of Medical Research. 1971 Feb; 59(2): 307-10. ...
Early History and Chemical Methods (Fractionation). Blood was first emphasized diagnostically by Hippocrates, who proposed that ... in which chemical fractionation based on protein affinity for derivatized MS targets is used to reduce sample complexity to a ... the era of fractionation (chemical methods), 3) the era of enzymes (biochemical methods), 4) the era of monoclonal antibodies ( ... One was the group of Cohn (6) and Edsall working on the preparation and fractionation of plasma; during the Second World War, ...
Chemical derivatization and fractionation of enriched peptides.. Eight times the bead volume of BEMAD buffer (1.5% ... iTRAQ labeling and fractionation of peptides.. Peptides from the flow-through and three washes of the avidin columns were ... The BEMAD chemical derivatization was performed directly on the solid phase after the tagged peptides were captured by avidin ... Kolb H, Finn M, Sharpless K: Click chemistry: diverse chemical function from a few good reactions. Angew Chem40 :2004 -2021, ...
Two of these methods are foam fractionation, for the separation of molecular species, and… ... Foam fractionation and flotation: There are a few methods that employ foams to achieve separations. In these, the principle of ... Other articles where Foam fractionation is discussed: separation and purification: ... chemical separations. * In separation and purification: Foam fractionation and flotation. There are a few methods that employ ...
Find details about China Chiller for Chemical Industry, Chiller for Fractional Distillation from Air Cooled Chiller for ... Fractionation of Oil - Zhejiang Kingfit Environment Co., Ltd. ... China Air Cooled Chiller for Fractionation of Oil, ... Chiller for Chemical Industry, Chiller for Fractional Distillation, Chiller for Distil manufacturer / supplier in China, ... CE Approved 10RT Air Cooled Chiller for Fractionation of Oil.. Model: QLB40FC;. Cooling capacity: 10RT (35KW);. Inlet chilled ...
Chemical fractionation of Cu and Zn was carried out in the soil of the 0.0-0.05, 0.05-0.10 and 0.10-0.20m layers (Tessier et al ... and chemical fractionation, which estimates soluble fraction, exchangeable fraction, fraction associated with clay minerals, ... Methods for chemical analysis are used to estimate the Cu and Zn content in the soil. The total content of the two trace ... The physical and chemical attributes were determined in the 0.0-0.05, 0.05-0.10 and 0.10-0.20m layers. Analysis of ...
This technique is called field-flow fractionation. It has been termed one-phase chromatography because there is no stationary ... Other articles where Field-flow fractionation is discussed: chromatography: Subsequent developments: ... chemical separation and purification. *. In chromatography: Subsequent developments. This technique is called field-flow ... Field-flow fractionation consists of a series of methods based on a field applied perpendicular to a flow stream in a narrow ...
Chemical Fractionation; Proximate Analysis; Ash Fusion Temperatures; Chemical Fractionation; Ash Chemistry, etc. Biomass ... Biochemistry ~ Chemical Engineering ~ Chemical Physics Chemical Senses ~ Chemistry of Brewing ~ Ceramics & Clays Clinical ... Chemicals FOR OVER 100 CHEMICAL DATABASES SEE THE CHEMICALS & BIOCHEMICALS First Year Chemistry: Courses,. Lectures, ... Journal of the American Chemical Society Chemical Research in Toxicology Analytical Chemistry Chemical & Engineering News ...
A novel fractionation method, based on both particle size and settling characteristics, was employed to examine metal ... Chemical Fractionation / instrumentation*. Environmental Monitoring / instrumentation*. Equipment Design. Fresh Water / ... A novel fractionation method, based on both particle size and settling characteristics, was employed to examine metal ...
Biorefinary products Cellulosic bioethanol Biplastics Biodiesel Pr Fine and bulk Biogas ab chemicals Syngas ud Oleo chemicals ... Biorefinary (Technology Base ) Petroleum Refineries Biorefinary Pr a. Distillation a. Biomass fractionation ab b. Thermo ... ud We are in talk with Agro Chemicals Policy group dh to implement Biofertilizer and Biopesticides in M chemical manufacturing ... We can also make biofertilizer from Blue Green Algae . Pr ab Some companies are involve in making oleo ud chemicals from algae ...
Subcellular fractionation and chemical crosslinking. Request a detailed protocol Cells were cultured on a 10 cm dish to 80-90% ... subcellular fractionation combined with chemical crosslinking was performed. In preliminary experiments with recombinant ... Chemical crosslinking in vitro. Request a detailed protocol 5 µM of recombinant CHIP were crosslinked with 0.025% ... 20 × 106 cells/ml suspension was lysed in subcellular fractionation buffer (SF: 250 mM sucrose, 20 mM HEPES KOH pH 7.5, 10 mM ...
54 ENVIRONMENTAL SCIENCES; 58 GEOSCIENCES; 14C; Soil carbon stabilization; Selective chemical dissolution; Soil fractionation; ... Chemical Geology Here, soil erosion continuously redistributes soil and associated soil organic matter (SOM) on the Earths ... the amount of Fe and Al in operationally-defined poorly crystalline and organically-complexed phases using selective chemical ... the amount of Fe and Al in operationally-defined poorly crystalline and organically-complexed phases using selective chemical ...
Chemical composition distribution (CCD). Crystallization analysis fractionation (Crystaf). Modeling. Olefin block copolymers ( ... A study on chemical composition distribution (CCD) of linear low density polyethylene (LLDPE) using crystallization analysis ... A study on chemical composition distribution (CCD) of linear low density polyethylene (LLDPE) using crystallization analysis ... fractionation (Crystaf) Title alternative การศึกษาการกระจ
Toxicity and mutagenicity of Upper Danube River sediments determined by chemical fractionation, the Danio rerio embryo assay, ... Fractionation of each of the four sediment samples was performed by separating compounds according to their polarity, planarity ... In this study, four sediment extracts from the Upper Danube River in Germany were used with a novel fractionation technique to ... Taken as a whole, these results show the value of using multiple bioassays and fractionation to characterize sediments that ...
Relation between Physical-Chemical Protein Properties and Fractionation/Identification. We analyzed several of the physical- ... Linking the Physical-Chemical Properties of the Identified Processed (Mature) Proteins to the Sequential Fractionation. ... Details about protein fractionation can be extracted for those proteins that are identified experimentally by our lab. When ... Physical-chemical properties were calculated for processed proteins, based on cleavage site prediction by TargetP (for the cTPs ...
3. Isolation and fractionation. 4. Physico-chemical characterisation. 5. Analysis of constituents. 6. Structural analysis. 7. ... and a thorough chemical overview of what can and cannot be done to the intact glycoproteins and their isolated glycopeptides. @ ...
A molecular zoom into soil Humeome by a direct sequential chemical fractionation of soil journal, May 2017 * Drosos, Marios; ... Chemical Diversity and Complexity of Scotch Whisky as Revealed by High-Resolution Mass Spectrometry journal, October 2016 * Kew ... Characterization of the chemical composition of soil humic acids using Fourier transform ion cyclotron resonance mass ... Reactivity and chemical characterization of effluent organic nitrogen from wastewater treatment plants determined by Fourier ...
1. Chemical fractionation and in vitro biological screening. Environ Sci Technol 32:1549-1558CrossRefGoogle Scholar ... Alvani K, Qi X, Tester RF, Snape CE (2011) Physico-chemical properties of potato starches. J Food Chem 125:958-965CrossRef ... Lei W, Si W, Xu Y, Gu Z, Hao Q (2014) Conducting polymer composites with graphene for use in chemical sensors and biosensors. ... Colborn T, vom Saal FS, Soto AM (1993) Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ ...
1. Chemical fractionation and in vitro biological screening. Environ Sci Technol 32(11):1549-1558CrossRefGoogle Scholar ... Desbrow C, Routledge E, Brightly G, Sumpter J, Waldock M (1998) Identification of estrogenic chemicals in STW effluent. ... of male clams Scrobicularia plana from estuaries in Southwest UK and its induction by endocrine-disrupting chemicals. Mar Ecol ...
  • Foam fractionation is a chemical process in which hydrophobic molecules are preferentially separated from a liquid solution using rising columns of foam. (wikipedia.org)
  • Whilst protein skimmers have been common place in aquaria for many years, it was not until the 1960s that a concerted effort was made by Robert Lemlich of the University of Cincinnati to characterise of model adsorptive bubble separation processes, of which foam fractionation is one example. (wikipedia.org)
  • Until the mid-2000s, there was very little further development of foam fractionation or attempts to understand the underlying physics of the process. (wikipedia.org)
  • Foam fractionation is closely related to the allied process of froth flotation in which hydrophobic particles attach to the surface of bubbles which rise to form a pneumatic (i.e. rising) foam. (wikipedia.org)
  • It was research into the froth phase of froth flotation conducted at the University of Newcastle, Australia, specifically into the prediction of liquid fraction and liquid flux in a pneumatic foam, that enabled a preliminary mechanistic description of foam fractionation. (wikipedia.org)
  • The synergies between foam fractionation and froth flotation have been explored in a 2009 special issue of the Asia Pacific Journal of Chemical Engineering. (wikipedia.org)
  • Robert Lemlich showed how foam fractionation columns can be operated in stripping, enriching, or combined modes (depending on whether the feed is sent to the top, bottom or middle of the column), and can be operated with or without an external reflux stream at the top of the column. (wikipedia.org)
  • Foam fractionation proceeds via two mechanisms: The target molecule adsorbs to a bubble surface, and The bubbles form a foam which travels up a column and is discharged to the foamate stream of foam fractionation. (wikipedia.org)
  • It is further divided into froth flotation and foam fractionation. (wikipedia.org)
  • Several techniques, collectively known as chemical filtration, are used for the removal of dissolved wastes, the most popular being the use of activated carbon and foam fractionation. (wikipedia.org)
  • In this study, four sediment extracts from the Upper Danube River in Germany were used with a novel fractionation technique to characterize the sediment extracts and fractions for their ability to disrupt steroidogenesis, for their mutagenic activities and their teratogenic effects. (usask.ca)
  • A novel fractionation approach for water constituents - distribution of storm event metals. (biomedsearch.com)
  • A novel fractionation method, based on both particle size and settling characteristics, was employed to examine metal distributions among five fractions. (biomedsearch.com)
  • Guidelines for terms related to chemical speciation and fractionation of elements. (hindawi.com)
  • Chemical speciation studies in real systems are usually very complex, due to the wide number of interactions that must be taken into account, which lead to the formation of several species of different stability [ 1 - 7 ]. (hindawi.com)
  • Unfortunately, the simple preparation and use of an artificial medium is not sufficient when performing rigorous chemical speciation studies. (hindawi.com)
  • In other words, a chemical speciation model of the biological fluid itself is necessary prior to any investigation on the speciation of any other component in that fluid. (hindawi.com)
  • This paper also claims that neither the measurement of total concentrations (reliable methods are lacking for many elements of the periodic table of trace elements, as illustrated through the cases of Bi, Te, and Sb), nor chemical speciation analysis, are as mature as often thought. (mdpi.com)
  • In particular, chemical speciation studies demand the development of a better, comprehensive conceptual framework. (mdpi.com)
  • Maggi F. and W. J. Riley, (2010), Mathematical treatment of isotopologue and isotopomer speciation and fractionation in biochemical kinetics, Geochim. (wikipedia.org)
  • APS JOURNALS ONLINE - American Physical Society Multimedia Chemical Physics Journals (Text & Images). (martindalecenter.com)
  • In a batch treatment system, a volume of wastewater is gathered, treated by chemical / physical / biological means, and discharged. (environmental-expert.com)
  • Physical and chemical properties were assessed, including aggregate stability, humic organic fractions, and a microbiological evaluation through determination of basal respiration, microbial biomass carbon, and metabolic quotient. (scielo.br)
  • The soils under study showed physical and chemical properties typical of a Haplustox, such as low nutrient content, low cation exchange capacity, and high levels of acidity and Al saturation. (scielo.br)
  • Threshold limit values for chemical substances and physical agents and biological exposure indices. (cdc.gov)
  • During convective bubble growth, the presence of a discrete (physical) melt boundary layer against the growing bubble (of width x BL) simplifies outcomes because it leads to the quick onset of steady-state fractionation during growth, the magnitude of which depends mainly upon R∙x BL/D L and D H/D L (bubble/melt fractionation is maximized at R∙x BL/D L ≈0.1). (deepdyve.com)
  • The types of synchronizations are broadly categorized into two groups: "Physical Fractionation" and "Chemical Blockade. (wikipedia.org)
  • Physical fractionation or cell separation techniques, based on the following characteristics are in use. (wikipedia.org)
  • Each genre of unit processing follows the same chemical law much as each genre of unit operations follows the same physical law. (wikipedia.org)
  • This differs from other separation techniques which depend upon chemical or physical interactions to separate analytes. (wikipedia.org)
  • Geobiologic studies tend to be focused on microorganisms, and on the role that life plays in altering the chemical and physical environment of the lithosphere, atmosphere, hydrosphere and/or cryosphere. (wikipedia.org)
  • Aquarium filters remove physical and soluble chemical waste products from aquaria, simplifying maintenance. (wikipedia.org)
  • Mikkel Frandsen (1892-1981) was a Danish American physical chemist noted for experiments involving chemical thermodynamics, oil, and heavy water. (wikipedia.org)
  • While teeth are more resistant to chemical and physical changes over time, both are subject to post-depositional diagenesis. (wikipedia.org)
  • and chemical fractionation, which estimates soluble fraction, exchangeable fraction, fraction associated with clay minerals, fraction associated with organic matter and residual fraction. (scielo.br)
  • Flavor base - oils can also "carry" flavors of other ingredients, since many flavors are due to chemicals that are soluble in oil. (wikipedia.org)
  • Adsorption removes a soluble impurity from a feed stream by trapping it on the surface of a solid material, such as activated carbon, that forms strong non-covalent chemical bonds with the impurity. (wikipedia.org)
  • For this, we provide cellular models that allow the screening and bio-guided fractionation of extracts aiming to isolate in vitro anti-inflammatory compounds (i.e. by luciferase reporter gene assays). (uibk.ac.at)
  • Because the amount of chemical sampled is directly related to the sample surface area, it is sometimes necessary to combine extracts from multiple POCIS disks into one sample. (wikipedia.org)
  • Taken as a whole, these results show the value of using multiple bioassays and fractionation to characterize sediments that covers a variety of different biological endpoints. (usask.ca)
  • The journal is abstracted and indexed in Biological Abstracts, Chemical Abstracts, Current Contents/Life Sciences, EMBiology, Food Science and Technology Abstracts, MEDLINE, Science Citation Index and Scopus. (wikipedia.org)
  • Sequential extraction procedures were used to assess selective chemical fractions of Pb in the soil. (mdpi.com)
  • Countercurrent distribution is a separation process that is founded on the principles of liquid-liquid extraction where a chemical compound is distributed (partitioned) between two immiscible liquid phases (oil and water for example) according to its relative solubility in the two phases. (wikipedia.org)
  • In soils from four sites spanning three soil orders, we quantified the amount of Fe and Al in operationally-defined poorly crystalline and organically-complexed phases using selective chemical dissolution applied to the soil fraction containing mineral-associated carbon. (osti.gov)
  • A microchip laboratory system and method proved fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. (google.com)
  • In 1979, he shifted to academia, joining the University of Texas at Austin School of Chemical Engineering, where he founded the Separations Research Program, which he headed from 1982 until 1996. (wikipedia.org)
  • Erdemir US, Gucer S (2016a) Characterization of copper bioavailability in wheat flour by chemical fractionation and inductively coupled plasma-mass spectrometry. (springer.com)
  • The chemical potential gradients caused by the initial assemblage appear at the far end of the melt column because that is the finite boundary condition for the diffusive supply of ions to the crystallization front. (arizonageologicalsoc.org)
  • Hiserodt RD, Swijter DFH, Mussinan CJ (2000) Identification of atranorin and related potential allergens in oak moss absolute by high-performance liquid chromatography-tandem mass spectrometry using negative ion atmospheric pressure chemical ionization. (springer.com)
  • Theories suggest that the uplift of the Tibetan Plateau has resulted in stronger deflections of the atmospheric jet stream, a heavier monsoonal circulation, increased rainfall on the front slopes, greater rates of chemical weathering, and thus lower atmospheric CO2 concentrations. (wikipedia.org)
  • Fractionation which separates the olein (liquid) and stearin (solid) fractions of oil is used to modify the physicochemical properties of fats in order to extend its applications. (peerj.com)
  • A common trait in fractionations is the need to find an optimum between the amount of fractions collected and the desired purity in each fraction. (wikipedia.org)
  • In such refineries, the crude oil feedstock is a complex, multicomponent mixture that must be separated, and yields of pure chemical compounds are not expected, only groups of compounds within a relatively small range of boiling points, also called fractions. (wikipedia.org)
  • Toxicity and mutagenicity of Upper Danube River sediments determined by chemical fractionation, the Danio rerio embryo assay, the Ames fluctuation test and the H295R assay. (usask.ca)
  • This raises the possibility that the character of volatile components in bubbles may not reflect that of volatiles dissolved in the host melt over the lifetime of a bubble-even in the absence of equilibrium vapor/melt isotopic fractionation. (deepdyve.com)
  • Relative volatility is a measure comparing the vapor pressures of the components in a liquid mixture of chemicals. (wikipedia.org)