Chaperonin Containing TCP-1: A group II chaperonin found in eukaryotic CYTOSOL. It is comprised of eight subunits with each subunit encoded by a separate gene. This chaperonin is named after one of its subunits which is a T-COMPLEX REGION-encoded polypeptide.Chaperonin 10: A group I chaperonin protein that forms a lid-like structure which encloses the non-polar cavity of the chaperonin complex. The protein was originally studied in BACTERIA where it is commonly referred to as GroES protein.Chaperonin 60: A group I chaperonin protein that forms the barrel-like structure of the chaperonin complex. It is an oligomeric protein with a distinctive structure of fourteen subunits, arranged in two rings of seven subunits each. The protein was originally studied in BACTERIA where it is commonly referred to as GroEL protein.Chaperonins: A family of multisubunit protein complexes that form into large cylindrical structures which bind to and encapsulate non-native proteins. Chaperonins utilize the energy of ATP hydrolysis to enhance the efficiency of PROTEIN FOLDING reactions and thereby help proteins reach their functional conformation. The family of chaperonins is split into GROUP I CHAPERONINS, and GROUP II CHAPERONINS, with each group having its own repertoire of protein subunits and subcellular preferences.t-Complex Genome Region: A 20 cM region of mouse chromosome 17 that is represented by a least two HAPLOTYPES. One of the haplotypes is referred to as the t-haplotype and contains an unusual array of mutations that affect embryonic development and male fertility. The t-haplotype is maintained in the gene pool by the presence of unusual features that prevent its recombination.Group II Chaperonins: A subcategory of chaperonins found in ARCHAEA and the CYTOSOL of eukaryotic cells. Group II chaperonins form a barrel-shaped macromolecular structure that is distinct from GROUP I CHAPERONINS in that it does not utilize a separate lid like structure to enclose proteins.Thiosulfate Sulfurtransferase: An enzyme that catalyzes the transfer of the planetary sulfur atom of thiosulfate ion to cyanide ion to form thiocyanate ion. EC 2.8.1.1.Protein Folding: Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.Thermosomes: Group II chaperonins found in species of ARCHAEA.Group I Chaperonins: A subcategory of chaperonins found in MITOCHONDRIA; CHLOROPLASTS; and BACTERIA. Group I chaperonins form into a barrel-shaped macromolecular structure that is enclosed by a separate lid-like protein component.Malate Dehydrogenase: An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC 1.1.1.37.Gram-Positive Asporogenous Rods, Irregular: A group of irregular rod-shaped bacteria that stain gram-positive and do not produce endospores.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Archaeal Proteins: Proteins found in any species of archaeon.Protein Refolding: Conformational transitions of a protein from unfolded states to a more folded state.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Heat-Shock Proteins: Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Molecular Chaperones: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.Chlorophenols: Phenols substituted with one or more chlorine atoms in any position.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Ribulose-Bisphosphate Carboxylase: A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.Methanococcus: A genus of anaerobic coccoid METHANOCOCCACEAE whose organisms are motile by means of polar tufts of flagella. These methanogens are found in salt marshes, marine and estuarine sediments, and the intestinal tract of animals.Thermococcus: A genus of extremely thermophilic heterotrophic archaea, in the family THERMOCOCCACEAE, occurring in heated sea flows. They are anaerobic chemoorganotropic sulfidogens.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Protein Denaturation: Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.Sulfolobus: A genus of aerobic, chemolithotrophic, coccoid ARCHAEA whose organisms are thermoacidophilic. Its cells are highly irregular in shape, often lobed, but occasionally spherical. It has worldwide distribution with organisms isolated from hot acidic soils and water. Sulfur is used as an energy source.Bacterial Proteins: Proteins found in any species of bacterium.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Protein Subunits: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.Cryoelectron Microscopy: Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Eukaryotic Cells: Cells of the higher organisms, containing a true nucleus bounded by a nuclear membrane.Adenylyl Imidodiphosphate: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.Tubulin: A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Alcohol Dehydrogenase: A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.3-Isopropylmalate Dehydrogenase: An NAD+ dependent enzyme that catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate to 3-carboxy-4-methyl-2-oxopentanoate. It is involved in the biosynthesis of VALINE; LEUCINE; and ISOLEUCINE.Kinetics: The rate dynamics in chemical or physical systems.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Thermus thermophilus: A species of gram-negative, aerobic, rod-shaped bacteria found in hot springs of neutral to alkaline pH, as well as in hot-water heaters.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Protein Renaturation: The reconstitution of a protein's activity following denaturation.Aeropyrum: A genus of anaerobic, chemolithotropic coccoid ARCHAEA, in the family DESULFUROCOCCACEAE. They live in marine environments.Vibrio cholerae: The etiologic agent of CHOLERA.Citrate (si)-Synthase: Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Calcium Phosphates: Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Tranylcypromine: A propylamine formed from the cyclization of the side chain of amphetamine. This monoamine oxidase inhibitor is effective in the treatment of major depression, dysthymic disorder, and atypical depression. It also is useful in panic and phobic disorders. (From AMA Drug Evaluations Annual, 1994, p311)Substrate Cycling: A set of opposing, nonequilibrium reactions catalyzed by different enzymes which act simultaneously, with at least one of the reactions driven by ATP hydrolysis. The results of the cycle are that ATP energy is depleted, heat is produced and no net substrate-to-product conversion is achieved. Examples of substrate cycling are cycling of gluconeogenesis and glycolysis pathways and cycling of the triglycerides and fatty acid pathways. Rates of substrate cycling may be increased many-fold in association with hypermetabolic states resulting from severe burns, cold exposure, hyperthyroidism, or acute exercise.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Microtubule-Associated Proteins: High molecular weight proteins found in the MICROTUBULES of the cytoskeletal system. Under certain conditions they are required for TUBULIN assembly into the microtubules and stabilize the assembled microtubules.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Methanobacterium: A genus of anaerobic, rod-shaped METHANOBACTERIACEAE. Its organisms are nonmotile and use ammonia as the sole source of nitrogen. These methanogens are found in aquatic sediments, soil, sewage, and the gastrointestinal tract of animals.Multiprotein Complexes: Macromolecular complexes formed from the association of defined protein subunits.Anilino Naphthalenesulfonates: A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. They are frequently used as fluorescent dyes and sulfhydryl reagents.Guanidine: A strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant. (From Martindale, the Extra Pharmacopoeia, 30th ed and Merck Index, 12th ed) It is also used in the treatment of myasthenia and as a fluorescent probe in HPLC.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Thermoplasma: A genus of facultatively anaerobic heterotrophic archaea, in the order THERMOPLASMALES, isolated from self-heating coal refuse piles and acid hot springs. They are thermophilic and can grow both with and without sulfur.PropaneEnzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Tritolyl Phosphates: A mixture of isomeric tritolyl phosphates. Used in the sterilization of certain surgical instruments and in many industrial processes.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.HSP70 Heat-Shock Proteins: A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Isocitrate Dehydrogenase: An enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. (From Dorland, 27th ed) (The NADP+ enzyme is EC 1.1.1.42.) EC 1.1.1.41.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Spectrometry, Fluorescence: Measurement of the intensity and quality of fluorescence.Tetrahydrofolate Dehydrogenase: An enzyme of the oxidoreductase class that catalyzes the reaction 7,8-dihyrofolate and NADPH to yield 5,6,7,8-tetrahydrofolate and NADPH+, producing reduced folate for amino acid metabolism, purine ring synthesis, and the formation of deoxythymidine monophosphate. Methotrexate and other folic acid antagonists used as chemotherapeutic drugs act by inhibiting this enzyme. (Dorland, 27th ed) EC 1.5.1.3.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Allosteric Regulation: The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.

Origin of gene overlap: the case of TCP1 and ACAT2. (1/158)

The human acetyl-CoA acetyltransferase 2 gene, ACAT2, codes for a thiolase, an enzyme involved in lipid metabolism. The human T-complex protein 1 gene, TCP1, encodes a molecular chaperone of the chaperonin family. The two genes overlap by their 3'-untranslated regions, their coding sequences being located on opposite DNA strands in a tail-to-tail orientation. To find out how the overlap might have arisen in evolution, the homologous genes of the zebrafish, the African toad, caiman, platypus, opossum, and wallaby were identified. In each species, standard or long polymerase chain reactions were used to determine whether the ACAT2 and TCP1 homologs are closely linked and, if so, whether they overlap. The results reveal that the overlap apparently arose during the transition from therapsid reptiles to mammals and has been retained for >200 million years. Part of the overlapping untranslated region shows remarkable sequence conservation. The overlap presumably arose during the chromosomal rearrangement that brought the two unrelated and previously separated genes together. One or both of the transposed genes found by chance signals that are necessary for the processing of their transcripts to be present on the noncoding strand of the partner gene.  (+info)

Disassembly of the cytosolic chaperonin in mammalian cell extracts at intracellular levels of K+ and ATP. (2/158)

The eukaryotic, cytoplasmic chaperonin, CCT, is essential for the biogenesis of actin- and tubulin-based cytoskeletal structures. CCT purifies as a doubly toroidal particle containing two eight-membered rings of approximately 60-kDa ATPase subunits, each encoded by an essential and highly conserved gene. However, immunofluorescence detection with subunit-specific antibodies has indicated that in cells CCT subunits do not always co-localize. We report here that CCT ATPase activity is highly dependent on K+ ion concentration and that in cell extracts, at physiological levels of K+ and ATP, there is considerable dissociation of CCT to a smaller oligomeric structure and free subunits. This dissociation is consequent to ATP hydrolysis and is readily reversed on removal of ATP. The ranking order for ease with which subunits can exit the chaperonin particle correlates well with the length of a loop structure, identified by homology modeling, in the intermediate domain of CCT subunits. K+-ATP-induced disassembly is not an intrinsic property of purified CCT over a 40-fold concentration range and requires the presence of additional factor(s) present in cell extracts.  (+info)

Structure of Tetrahymena CCT theta gene and its expression under colchicine treatment. (3/158)

We report here the cloning and the characterization of the Tetrahymena pyriformis chaperonin-containing-TCP1 theta gene (TpCCT theta), an orthologue of the mouse chaperonin gene CCT theta. TpCCT theta gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative pI of 5.73. The amino acid sequence of TpCCT theta reveals 39.4-46.0% identity with the sequences of Candida albicans and mouse CCT theta subunits and 28.0-32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCT theta mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.  (+info)

Transcriptional activation of mouse cytosolic chaperonin CCT subunit genes by heat shock factors HSF1 and HSF2. (4/158)

The chaperonin containing TCP-1 (CCT) is a eukaryotic molecular chaperone consisting of eight subunit species and assists in the folding of cytosolic proteins. We show here that all eight mouse CCT subunit genes contain sequences called heat shock elements for binding heat shock transcription factors (HSFs) by electrophoretic mobility shift assays and that these genes are transcriptionally activated by HSFs in reporter gene assays using HeLa cells transiently overexpressing HSFs. These results suggest that HSF1 and/or HSF2 play a role in Cct gene expression.  (+info)

Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase. (5/158)

The chaperonin containing t-complex polypeptide 1 (CCT) is a heterooligomeric molecular chaperone assisting in the folding of actin, tubulin, and other cytosolic proteins. The expression levels of CCT subunits varied among seven mouse cell lines tested but showed a close correlation with growth rate. Both the CCT protein and mRNA levels in the human promyelolytic cell HL60 decreased concomitant with growth arrest during differentiation. More rapid decrease in CCT level occurred when the mouse interleukin (IL)-3-dependent myeloid DA3 cells were starved for IL-3. Readdition of IL-3 caused rapid resumption of CCT synthesis during synchronous growth: the maximum CCT protein and mRNA levels were observed at G(1)/S transition through early S phase. The turnover rate of CCT was nearly constant regardless of growth. Gel filtration and immunoprecipitation analyses indicated that CCT in vivo is associated with tubulin at early S phase, but not at G(0)/G(1) phase. These results demonstrated that CCT expression is strongly up-regulated during cell growth especially from G(1)/S transition to early S phase and is primarily controlled at the mRNA level. CCT appears to play important roles for cell growth by assisting in the folding of tubulin and other proteins.  (+info)

Partial occlusion of both cavities of the eukaryotic chaperonin with antibody has no effect upon the rates of beta-actin or alpha-tubulin folding. (6/158)

The eukaryotic chaperonin containing T-complex polypeptide 1 (CCT) is required in vivo for the production of native actin and tubulin. It is a 900-kDa oligomer formed from two back-to-back rings, each containing eight different subunits surrounding a central cavity in which interactions with substrates are thought to occur. Here, we show that a monoclonal antibody recognizing the C terminus of the CCTalpha subunit can bind inside, and partially occlude, both cavities of apo-CCT. Rabbit reticulocyte lysate was programmed to synthesize beta-actin and alpha-tubulin in the presence and absence of anti-CCTalpha antibody. The binding of the antibody inside the cavity and its occupancy of a large part of it does not prevent the folding of beta-actin and alpha-tubulin by CCT, despite the fact that all the CCT in the in vitro translation reactions was continuously bound by two antibody molecules. Furthermore, no differences in the protease susceptibility of actin bound to CCT in the presence and absence of the monoclonal antibody were detected, indicating that the antibody molecules do not perturb the conformation of actin folding intermediates substantially. These data indicate that complete sequestration of substrate by CCT may not be required for productive folding, suggesting that there are differences in its folding mechanism compared with the Group I chaperonins.  (+info)

Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins. (7/158)

The chaperonin containing TCP-1 (CCT) is a molecular chaperone consisting of eight subunit species and assists in the folding of actin, tubulin and some other cytosolic proteins. We examined the stress response of CCT subunit proteins in mammalian cultured cells using chemical stressors that cause accumulation of unfolded proteins. Levels of CCT subunit proteins in HeLa cells were coordinately and transiently upregulated under continuous chemical stress with sodium arsenite. CCT subunit levels in several mammalian cell lines were also upregulated during recovery from chemical stress caused by sodium arsenite or a proline analogue, L-azetidine-2-carboxylic acid. Several unidentified proteins that were newly synthesized and associated with CCT were found to increase concomitantly with CCT subunits themselves and known substrates during recovery from the stress. These results suggest that CCT plays important roles in the recovery of cells from protein damage by assisting in the folding of proteins that are actively synthesized and/or renatured during this period.  (+info)

Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. (8/158)

The chaperonin containing TCP-1 (CCT) of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. CCT has been loaded with (35)S-beta-actin by in vitro translation in reticulocyte lysate and then subjected to immunoprecipitation with all eight anti-CCT subunit antibodies in mixed micelle buffers, conditions that disrupt CCT into its constituent monomers. Interactions between (35)S-beta-actin and isolated CCTalpha, CCTbeta, CCTepsilon, or CCTtheta subunits are observed, suggesting that polar and electrostatic interactions may mediate actin binding to these four CCT subunits. Additionally, a beta-actin peptide array was screened for CCT-binding sequences. Three regions rich in charged and polar amino acid residues, which map to the surface of native beta-actin, are implicated in interactions between actin and CCT. Several of these biochemical results are consistent with the recent cryo-electron microscopy three-dimensional structure of apo-CCT-alpha-actin, in which alpha-actin is bound by the apical domains of specific CCT subunits. A model is proposed in which actin interacts with several CCT subunits during its CCT-mediated folding cycle.  (+info)

*CCT6B

Kubota H, Hynes GM, Kerr SM, Willison KR (Feb 1997). "Tissue-specific subunit of the mouse cytosolic chaperonin-containing TCP- ... "Entrez Gene: CCT6B chaperonin containing TCP1, subunit 6B (zeta 2)". Human CCT6B genome location and CCT6B gene details page in ... This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the ... T-complex protein 1 subunit zeta-2 is a protein that in humans is encoded by the CCT6B gene. ...

*CCT3

"Entrez Gene: CCT3 chaperonin containing TCP1, subunit 3 (gamma)". Chen GI, Tisayakorn S, Jorgensen C, D'Ambrosio LM, Goudreault ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (Feb 1994). "Identification of six Tcp-1-related genes encoding divergent ... This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ... which encodes the chaperonin subunit CCT gamma". The Biochemical Journal. 313 (Pt 2): 381-9. doi:10.1042/bj3130381. PMC 1216920 ...

*CCT8

... encoding the theta subunit of the cytosolic chaperonin containing TCP-1". Gene. 154 (2): 231-6. doi:10.1016/0378-1119(94)00880- ... "Entrez Gene: CCT8 chaperonin containing TCP1, subunit 8 (theta)". Human CCT8 genome location and CCT8 gene details page in the ... Yokota S, Yanagi H, Yura T, Kubota H (2001). "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a ... Hynes GM, Willison KR (2000). "Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding ...

*CCT7

Hynes G, Celis JE, Lewis VA, Carne A, U S, Lauridsen JB, Willison KR (Nov 1996). "Analysis of chaperonin-containing TCP-1 ... "Entrez Gene: CCT7 chaperonin containing TCP1, subunit 7 (eta)". Chen GI, Tisayakorn S, Jorgensen C, D'Ambrosio LM, Goudreault M ... This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (Feb 1994). "Identification of six Tcp-1-related genes encoding divergent ...

*CCT4

"Chromosomal assignment and imprinting tests for the mouse delta subunit of the cytosolic chaperonin containing TCP-1 (Cct4) ... "Cytoplasmic chaperonin containing TCP-1: structural and functional characterization". Biochemistry. 36 (19): 5817-26. doi: ... "Entrez Gene: CCT4 chaperonin containing TCP1, subunit 4 (delta)". Chen GI, Tisayakorn S, Jorgensen C, D'Ambrosio LM, Goudreault ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (Feb 1994). "Identification of six Tcp-1-related genes encoding divergent ...

*CCT5 (gene)

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (Feb 1994). "Identification of six Tcp-1-related genes encoding divergent ... Yokota S, Yanagi H, Yura T, Kubota H (Sep 2001). "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content ... Roobol A, Holmes FE, Hayes NV, Baines AJ, Carden MJ (Apr 1995). "Cytoplasmic chaperonin complexes enter neurites developing in ...

*CCT6A

"Entrez Gene: CCT6A chaperonin containing TCP1, subunit 6A (zeta 1)". Chen GI, Tisayakorn S, Jorgensen C, D'Ambrosio LM, ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (1994). "Identification of six Tcp-1-related genes encoding divergent ... This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ... Yokota S, Yanagi H, Yura T, Kubota H (2001). "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a ...

*CCT2 (gene)

"Entrez Gene: CCT2 chaperonin containing TCP1, subunit 2 (beta)". Chen GI, Tisayakorn S, Jorgensen C, D'Ambrosio LM, Goudreault ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (Feb 1994). "Identification of six Tcp-1-related genes encoding divergent ... This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ... Yokota S, Yanagi H, Yura T, Kubota H (Sep 2001). "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content ...

*Evolution of molecular chaperones

Gupta, RS (January 1995). "Evolution of the chaperonin families (Hsp60, Hsp10 and Tcp-1) of proteins and the origin of ... doi:10.1046/j.1461-0248.2003.00528.x. Williams, T. A.; Fares, M. A. (21 July 2010). "The Effect of Chaperonin Buffering on ... Phylogenetic analysis using two families of HSPs (hsp10 and hsp60, also called chaperonins) support the current endosymbiosis ...

*HSP60

Gupta RS (January 1995). "Evolution of the chaperonin families (Hsp60, Hsp10 and Tcp-1) of proteins and the origin of ... Alternate Names: 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, HuCHA60, Mitochondrial matrix protein ... this is not the case with chaperonins". It has been found that many anti-chaperonin antibodies exist and are associated with ... "generated by a human host after exposure to bacterial chaperonin 60 proteins" can cross-react with human chaperonin 60 proteins ...

*T-complex 1

Bakthavatsalam D, Soung RH, Tweardy DJ, Chiu W, Dixon RA, Woodside DG (Jun 2014). "Chaperonin-containing TCP-1 complex directly ... This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the ... Chen X, Sullivan DS, Huffaker TC (1994). "Two yeast genes with similarity to TCP-1 are required for microtubule and actin ... Kubota H, Hynes G, Carne A, Ashworth A, Willison K (1994). "Identification of six Tcp-1-related genes encoding divergent ...

*Chaperonin

Group II chaperonins, found in the eukaryotic cytosol and in archaea, are more poorly characterized. TRiC (TCP-1 Ring Complex, ... In archaea, the chaperonin is called the thermosome. In eukarya, the chaperonin is called CCT (also called TRiC or c-cpn). ... The active chaperonin role is in turn involved with specific chaperonin-substrate interactions that may be coupled to ... more details... Chaperonins at the US National Library of Medicine Medical Subject Headings (MeSH) cpnDB: a chaperonin database ...

*Actin

Brackley KI, Grantham J (Jan 2009). "Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle ... After AMP-PNP is bound to CCT the substrates move within the chaperonin's cavity. It also seems that in the case of actin, the ... The actin is recognized, loaded and delivered to the cytosolic chaperonin (CCT) in an open conformation by the inner end of ... CCT is a group II cytosolic molecular chaperone (or chaperonin, a protein that assists in the folding of other macromolecular ...

*TCP-1/cpn60 chaperonin family

The 10 kDa chaperonin (cpn10 - or groES in bacteria) exists as a ring-shaped oligomer of between 6 and 8 identical subunits, ... The TCP-1 family of proteins act as molecular chaperones for tubulin, actin and probably some other proteins. They are weakly, ... TCP-1/cpn60 chaperonin family is a family of evolutionarily related proteins. This family includes members from the HSP60 ... This chaperonin complex is essential for the correct folding and assembly of polypeptides into oligomeric structures, of which ...
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The substrate recognition mechanisms in chaperonins Chaperonins are a family of proteins devoted to assisting the folding of other proteins. They are large oligomers assembled into ring structures that enclose a cavity in which folding takes place. For this process to occur, the chaperonin must first recognize and interact with the unfolded polypeptide, then undergo a conformational change upon nucleotide binding that results in the closure of the cavity which in turn mediates the folding reaction inside the cavity. Although this general mechanism seems to apply to every chaperonin studied so far, there exist two different modes of interaction between the chaperonin and the substrate. The first occurs mainly through the interaction between the exposed hydrophobic residues of the unfolded polypeptides and those of the chaperonin substrate binding site, as elucidated for the chaperonin GroEL from E. coli. The second type of mechanism has been described so far only for the cytosolic chaperonin CCT ...
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Here, we study and compare the mechanisms of action of the GroEL/GroES and the TRiC chaperonin systems on MreB client protein variants extracted from E. coli. MreB is a homologue to actin in prokaryotes. Single-molecule fluorescence correlation spectroscopy (FCS) and time-resolved fluorescence polarization anisotropy report the binding interaction of folding MreB with GroEL, GroES and TRiC. Fluorescence resonance energy transfer (FRET) measurements on MreB variants quantified molecular distance changes occurring during conformational rearrangements within folding MreB bound to chaperonins. We observed that the MreB structure is rearranged by a binding-induced expansion mechanism in TRiC, GroEL and GroES. These results are quantitatively comparable to the structural rearrangements found during the interaction of beta-actin with GroEL and TRiC, indicating that the mechanism of chaperonins is conserved during evolution. The chaperonin-bound MreB is also significantly compacted after addition of ...
Enhancers and Super-Enhancers Have an Equivalent Regulatory Role in Embryonic Stem Cells through Regulation of Single or Multiple Genes The authors employed a CRISPR/Cas9-mediated deletion approach to study the function of several enhancer clusters and isolated enhancers in mouse embryonic stem cells. [Genome Res] Abstract Somatic Increase of CCT8 Mimics Proteostasis of Human Pluripotent Stem Cells and Extends C. elegans Lifespan Investigators showed that human pluripotent stem cells exhibit increased assembly of the chaperonin TRiC/CCT complex, a mechanism induced by high levels of specific CCT subunits. [Nat Commun] Full Article , Press Release The Deubiquitinase USP21 Maintains the Stemness of Mouse Embryonic Stem Cells via Stabilization of Nanog The authors report that the deubiquitinase USP21 interacts with, deubiquitinates and stabilizes Nanog, and therefore maintains the protein level of Nanog in mouse ESCs (mESCs). Loss of USP21 results in Nanog degradation, mESCs differentiation and ...
Actin requires the chaperonin containing TCP1 (CCT), a hexadecameric ATPase essential for cell viability in eukaryotes, to fold to its native state. Following binding of unfolded actin to CCT, the cavity of the chaperone closes and actin is folded and released in an ATP-dependent folding cycle. In yeast, CCT forms a ternary complex with the phosducin-like protein PLP2p to fold actin, and together they can return nascent or chemically denatured actin to its native state in a pure in vitro folding assay. The complexity of the CCT-actin system makes the study of the actin folding mechanism technically challenging. We have established a novel spectroscopic assay through selectively labeling the C terminus of yeast actin with acrylodan and observe significant changes in the acrylodan fluorescence emission spectrum as actin is chemically unfolded and then refolded by the chaperonin. The variation in the polarity of the environment surrounding the fluorescent probe during the unfolding/folding ...
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Build: Sat Feb 17 08:59:16 EST 2018 (commit: 16064c5). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Principal Investigator:KUWAJIMA Kunihiro, Project Period (FY):2008 - 2012, Research Category:Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Project Area:Molecular Science of Fluctuations toward Biological Functions
CCT4 Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. Note: This description may include information from UniProtKB ...
The Penticton Vees (14-5-1-2) hit double digits in goals in back to back games for the first time since October 2014 with a 10-1 drubbing of the Cowichan Valley Capitals (2-18-3-1) Saturday night.. Just 22 seconds into the game a shot from the right point by Ryan OConnell hit Jack Barnes in the skate and deflected past Capitals goalie Adam Marcoux to give the Vees the lead.. Jared Nash set a new career high with his sixth goal of the season to give the Vees a 2-0 lead just four minutes later after tapping in a centring feed from Taylor Ward.. Not long after, the Vees were hit with back-to-back unsportsmanlike penalties, putting them down two men. Ty Pochipinski scored with just one second remaining in the five on three to cut the lead down to 2-1.. Nash was clipped with a high stick off a face-off in the attacking zone that went undetected by the officials. Vees head coach Fred Harbinson voiced his displeasure and was ejected from the game.. Grant Cruikshank scored a short-handed goal just ...
Scheduling conflicts forced the Washington Capitals and Boston Bruins to play Games 5 and 6 back-to-back this weekend at TD Garden and then Verizon Center, but just about everyone involved brushed it off as any kind of a problem.
On June 14 the D.C. Bar CLE Program will offer back-to-back sessions on the opioid crisis and its impact on the legal profession, including substance misuse by attorneys.
Gene Information This gene is specifically expressed in the nucleus of haploid male germ cells. The orthologous gene in mice encodes a protein that may play a role in protein assembly through interactions with T-complex protein 1 subunit epsilon. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Nov 2011]. ...
1) The Sixers led by just three points after the first quarter, but the Knicks came out freezing cold in the second, shooting 1-of-11 from the floor to start the quarter. The Knicks fought back, though, tying the game 45-45 with two and a half minutes left in the first half. The Sixers finished the first half strong, winding up with a 52-46 lead at the break. The Knicks shot just 40.5 percent as a team and turned the ball over 10 times in the half.. Both teams came out sloppy in the second half, but the Knicks outscored the Sixers 23-18 in the frame, and trailed by just one point entering the fourth quarter. With four minutes to go in the game, the Sixers maintained an 84-81 lead. With just under two minutes to go, a Mitchell Robinson dunk cut the Sixers lead to just one point.. In the final minute. Marcus Morris knocked down a mid-range jumper to put the Knicks ahead 87-86 with 30.9 seconds left. Immediately, Tobias Harris knocked down a three to put the Sixers back in the lead 89-87. The ...
Tigres UANL have it all to do when they travel to Estadio Azteca for the return leg of their CONCACAF Champions League final against America on Wednesday. Tigres hosted the first leg but suffered a 2-0 defeat after conceding second-half goals from
Corey Crawford fights off Luke Glendenings shot with his glove, then extends a pad to rob Riley Sheahans rebound try on the Red Wings rush
Johann Zarco will graduate to MotoGP with Tech3 Yamaha next year as a two-time Moto2 champion, after securing the 2016 title with a commanding Sepang victory
Next was at the top of the aptly named "Vomit Hill," which is not terribly long but is ridiculously steep. When I reached the top, I was certain I was hallucinating; I had to take a video to confirm there was a woman playing a trumpet and offering riders donuts and PBR. And if you also dont believe me, heres the video to prove it.. The next bright spot was riding up Keystone road, where the pedals seemed to turn over easily. That lasted about 20 minutes, until I picked up an anchor, hugged it, and threw it overboard, dragging it all the way to the line. I dont think the small portion of donut I ate helped my situation, but I also believe my low energy was a result of being slightly underfed, under-slept and under-watered from the previous day. Thus, Ive eaten more, slept more and consumed more fluids today in an attempt to help my general well being.. Since Im on a rant of glass half-full stuff, I also kept air in my tires all day today, which is a big plus. No forest creatures need be ...
On the premiere of WSJs D.C. Bureau, Jerry Seib talks with President Obamas senior adviser David Axelrod and Mitt Romneys senior strategist Kevin Madden,
After a heads-up, no breakout final round, Ben Line claimed his second-straight Stock Eliminator victory at his home event, the Lucas Oil NHRA Nationals
Human CCT5 full-length ORF ( AAH06543, 1 a.a. - 541 a.a.) recombinant protein with GST-tag at N-terminal. (H00022948-P01) - Products - Abnova
FutureMed featured back-to-back sessions from Paul Grundy, MD and Watson guru Marty Kohn, MD. Well give you a rundown of both of their presentations here:
Thinking about making a move to Eagle s Trace? Don t wait. With only a handful of apartment homes available at the Erickson Living community in West Houston, time is running out to reserve the apartment home of your choice. I ve been booked with back-to-back appointments, says Sales Counselor and Personal Moving Consultant Blaga Bayer, who provides complimentary selling and moving help for people moving to Eagle s Trace.
As is normal for me, Ive gone and pushed my limits again. Not that Ive done anything crazy, nope rather simply training and riding my bike. After a heavy two week block it has become obvious that I cannot right now put together two hard/long back-to-back days. This is frustrating. This is not where I…
CESK1 antibody (chaperonin containing TCP1, subunit 8 (theta)-like 2) for IHC-P, WB. Anti-CESK1 pAb (GTX117858) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Members of this protein family are the archaeal form ofribosomal protein uL6 (previously L9 in yeast and human). The top-scoring proteins not selected by this model are eukaryotic cytosolic uL6. Bacterial ribosomal protein L6 scores lower and is described by a distinct model ...
What are the implications of the CCT-mediated actin folding mechanism for the structure and function of F-actin? Reisler & Egelman (2007) have collated many disparate observations concerning biochemical and structural properties of F-actin and argued convincingly that F-actin should not be considered to be a single state but rather a dynamic ensemble of many states. Transitions between states are probably coupled to rotations and tilts of subunits, which occur readily despite the apparently severe structural constraint of a constant axial rise of every subunit in the filament of approximately 27.3 Å. Put succinctly, in its breathing modes F-actin changes its shape more than its length.. The emerging view of time-correlated motions and protein function is that active enzymatic or ligand-binding states reflect configurations that pre-exist in the ensemble. In adenylate kinase, the catalytically competent closed state is occasionally sampled on microseconds-milliseconds time scales even in the ...
... is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008 ...
Trimeric intracellular cation-selective (TRIC) channel subtypes, namely TRIC-A and TRIC-B, are derived from distinct genes and distributed throughout the sarco/endoplasmic reticulum (SR/ER) and nuclear membranes. TRIC-A is preferentially expressed at high levels in excitable tissues, while TRIC-B is ubiquitously detected at relatively low levels in various tissues. TRIC channels are composed of ~300 amino acid residues and contain three putative membrane-spanning segments to form a bullet-shaped homo-trimeric assembly. Both native and purified recombinant TRIC subtypes form functional monovalent cation-selective channels in a lipid bilayer reconstitution system. The electrophysiological data indicate that TRIC channels behave as K(+) channels under intracellular conditions, although the detailed channel characteristics remain to be investigated. The pathophysiological defects detected in knockout mice suggest that TRIC channels support SR/ER Ca(2+) release mediated by ryanodine (RyR) and inositol
A method for packaging integrated circuit chips (die) is described that includes providing a base substrate with package level contacts, coating a base substrate with adhesive, placing dies on the adhesive, electrically connecting the die to the package level contacts, and removing the backside of the base substrate to expose the backside of the package level contacts. Accordingly, an essentially true chip scale package is formed. Multi-chip modules are formed by filling gaps between the chips with an encapsulant. In an embodiment, chips are interconnected by electrical connections between package level contacts in the base substrate. In an embodiment, substrates each having chips are adhered back-to-back with through vias formed in aligned saw streets to interconnect the back-to-back chip assembly.
There are several things that I really like about cardio block. One, Dr. Stewart is in charge again. He ran our cardio block last year too, and all I can say is that he is a really cool guy. I didnt come here particularly gung ho about cardiology, but he makes it hard not to get excited about it. Next, there is only one seminar per day instead of two. Some of the speakers still go over the page limit for the assigned reading, but one person over-assigning work to us is nowhere near as bad as two of them doing it each day! Plus, we arent crammed like sardines into the little conference rooms. Finally, the seminars weve had so far have been good, even the pharm one we had this morning. Yesterdays seminar was on hemodynamics, and it started out a little bit confusing, but it got better as it went on ...
It was crazy busy last night. Every time we thought we had it under control, it would explode again. EMS came in with back-to-back intubated patients, (both bleeds that got shipped to the Big Neuro Hospital in the Big City), a guy came in through the front door feeling "weak", (SVT at a rate of 195), back-to-back kids with asthma and sats in the 80s, and an aspirin/Unisom/Stacker2 OD that was WAY wiggin out. In between that was the usual smattering of chest pains, SOBs, broken bones, lacerations, ear infections, etc. The drug seekers, drunks and general pains in my ass were minimal. And as I ran around like a crazy monkey, I had a thought several times that I havent had for a loooooong time; "God I love this job!". ...
Louie Mechalides, of Tyngsboro took the lead on lap 51 and went on to win his second consecutive Seekonk 100 Saturday night, June 8, at Seekonk Speedway. Mechalides captured the June event last season.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
SWISS-MODEL is a fully automated protein structure homology-modelling server. The purpose of this server is to make protein modelling accessible to all life science researchers worldwide.
Complete information for CCT4P2 gene (Pseudogene), Chaperonin Containing TCP1 Subunit 4 Pseudogene 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for CCT4P1 gene (Pseudogene), Chaperonin Containing TCP1 Subunit 4 Pseudogene 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Recently, back-to-back bouts with two distinct flu viruses have managed to take hold of my habitual energy and squash it into a tiny, nearly non-existent ball of fluff. And as I was prostrated, I thought about how the body heals itself. And that, of course, led me to a favorite topic: the brain and its role in healing the heart and the emotions.. Rachel* came to see me the first time about ten years ago when she was in her mid-thirties. Her self-esteem was low. She was depressed, sad, and over-weight from stuffing herself with sugary products in a blind attempt to comfort eat her way to feeling better about herself. Recently out of a failed marriage that had eked away at her already low self-regard, she believed she was worthless and useless, despite rather amazing artistic abilities that I came to know more closely over time. She cried a lot during those early sessions.. Albert* came some years after Rachel, and was also plagued by deep feelings of lacking in self-esteem. He was just short of ...
Slides 4-5: after two votes there was no clear consensus, but most students likes A "no interactions between molecules". The second vote came at the end of the first of three back-to-back lectures, so we had a third vote after the break. There really was intense discussion of this during the break, when most finally settled on B "equal interactions between molecules". I think A was popular because "ideal solution" conjures up an analogy to "ideal gas", but how can you have a liquid if there are no intermolecular attractions ...
• Francona said outfielder Michael Bourn, who is expected to play back-to-back nine-inning games Thursday and Friday during his rehab assignment with Double-A Akron, is unlikely to join the team until Tuesday at the earliest for the Indians series at Detroit. Bourn is eligible to come off the disabled list Sunday. I wouldnt be surprised if its Tuesday, Francona said.
I gave two lectures back-to-back today, which I found a little stressful. The first lecture was a guest lecture in Molecular biomechanics on the basic of protein structure. I spent some time earlier this week picking out protein structure to show the students; digging out my old Darling models protein chain, which I last used…
Michigan State is trying to regain a share of first place in the Big Ten, while looking to further erase the memory of recent back-to-back losses.
Marathon running may trigger a cascade of potentially heart-damaging events, as reported by McLean Hospital researchers in back-to-back papers in the Oct. 17...
The Hudson back-to-back HVDC system and the Neptune HVDC system ensure stable power supply for the metropolitan area of New York.
Back-to-back rulings by a U.S. federal judge this week appear to be a legal first that specialists expect to prompt more lawsuits from people seeking a shorter wait
Jim Harbaughs San Francisco 49ers had never allowed 30 points in a regular-season game ... until two weeks ago. Now theyve given up 30-plus in back-to-back games. Bucky Brooks explains why.
UFC Hall of Famer and welterweight great Matt Hughes (fighting out of Hillsboro, Ill.) steps inside the Octagon® this fall in search of another signature win to add to his storied career. The 37-year-old former champion gets that opportunity when he meets the hard-charging Diego Sanchez (fighting out of Albuquerque, N.M.). Winner of season one of The Ultimate Fighter®, the 29-year-old Sanchez has developed a reputation for being a gritty, hard-nosed fighter with deceiving power and high-level grappling. Riding back-to-back wins since returning to the welterweight division Sanchez (25-4) looks for his third consecutive victory when he meets the legendary Hughes (46-8 ...
The adage of Easy-Hard is often applied to weekly training. Run easy one day, then do speedwork (very roughly, we could lump them all: tempo, interval, fartlek) the next. The Easy-Hard principle makes good sense. I think it can also be applied to back-to-back long runs, too. Just because a 5 hour run is done at an easy pace does not mean its an easy run. It needs to be put in context. If you run 4 hour runs all the time, maybe it is indeed easy. And if the day before and day after you run 10 or 15km (or not at all), maybe it is indeed easy. But if you run a 4 hour run and then wake up the next day to run another 4 hour run, can that second run REALLY be called easy? Only if it really feels bloody easy to you - and not to anyone else or any programs advice. That second run might really be a hard run ...
Because some fighters have more to lose than others. Anderson Silva: Back-to-back insomnia solutions ported Silva from professor to pariah overnight. If he finds a way to behave eccentrically against Forrest Griffin, the UFC is going to have to consider arming his next opponent. Jeff Curran: The WEC featherweight has lost three straight bouts against stiff competition: if he cant chamber a new career at 135 lbs. against Takeya Mizugaki, hes probably due for the Phone Call.â„¢
CCT244747 is a potent, orally bioavailable and highly selective CHK1 inhibitor, with an IC50 of 7.7 nM; CCT244747 also abrogates G2 checkpoint with an IC50 of 29 nM ...
TCP11L1兔多克隆抗体(ab121231)可与人样本反应并经WB, IHC, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
A discovery from the Currie Group at the Australian Regenerative Medicine Institute (ARMI), recently published in the high-impact journal Cell Reports, has shown a crucial role for TCP-1 ring complex (TRiC) in the formation of skeletal muscle and the hereditary neuromuscular disorder, nemaline myopathy. This work, led by Senior Research Fellow Dr Joachim Berger, provides novel insights into the genetic and molecular intricacies of muscle development.
The volunteer pool is apparently thin enough that Department of Corrections Director Wendy Kelley invited members of a local Rotary Club to volunteer.
Since Dr. van den Broeks posting Is Carl Woese losing a Kigngdom, and because a number of replies posted in response, are in reference to our work, I think it would be useful to clearly define the issues. The two main tenets of the current three domain phylogenetic view are: (i) Archaebacteria (or Archaea) constitutes a monophyletic domain which is completely distinct from the rest of the bacteria. (ii) Eukaryotic nuclear genome has directly descended from an Archaebacterial ancestor. However, both of these tenets, which were initially proposed based on either 16S rRNA data or elongation factor (EF-1,EF-2) sequences are not supported by other recent well characterized phylogenies. In terms of the relationship within the prokaryotes, if one examines all available protein sequences two common patterns are observed. (a) For a number of proteins that in general are not highly conserved in all prokaryotes (e.g. EF-1 /Tu, EF-2/G, RNA polymerase II and III subunits, GroEL/Tcp-1, etc.), the ...
CCT7 - CCT7 (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 7 (eta) (CCT7), transcript variant 3 available for purchase from OriGene - Your Gene Company.
Jungbluth and colleagues (22) generated monoclonal antibody 806 [mAb806(IgG2b)] by immunizing mice with murine NR6 fibroblasts expressing human EGFRvIII but not wtEGFR. Characterization of mAb806 confirmed binding to EGFRvIII, but, unexpectedly, mAb806 also bound to tumor cells overexpressing wtEGFR at a level in excess of 1 × 106 receptors per cell (23-26). In vivo studies confirmed that mAb806 bound to and reproducibly inhibited xenograft tumors that expressed EGFRvIII or overexpressed wtEGFR (19, 27-30). Of interest, no mAb806 binding was detected in normal tissues such as the liver (22).. The mechanism of mAb806′s tumor specificity was elucidated with the discovery that the epitope is located between amino acids 287 and 302 in the CR1 domain of the EGFR ECD (31, 32). This epitope is masked in the inactive monomer state (Fig. 1A) or the fully liganded back-to-back dimer state (Fig. 1B), which explains the lack of mAb806 binding to normal tissues in which EGFR is quiescent. However, the ...
He got a no-decision and the Red Sox lost, 3-2, with the bullpen picking up the loss.. Coming the night after the outfield defense behind starter Ryan Dempster (no decision) coughed up two unearned runs, costly errors that led directly to closer Joel Hanrahans pyrotechnically blown save and loss in the ninth, the back-to-back losses share a trait that should put this two-game dip in perspective.. The starters really did not have much to do with the loss. For any team, that counts as a win.. Of all the ways to lose, having the starting pitcher be responsible is worse than a bullpen loss, worse than a cold night from the bats, worse than a last-second defensive lapse.. To obtain the most accurate pulse of the health of a baseball team, head over to the rotation and begin there. Over the course of a long season, the Sox starting pitching is going to set the tone for everything that follows.. Right now, after nine starts, the Red Sox rotation has a 4-2 record with a 2.50 ERA. Thats a good ...
Avian Virus and Avian Immunology meetings will be held, back-to-back, in Oxford in September next year (2018).. Block the dates in your diaries, bookmark these webpages and make sure youre on the mailing lists!. Microbiology Society Focused Meeting: MOLECULAR BIOLOGY AND PATHOGENESIS OF AVIAN VIRUSES 2018. (3-4 Sept 2018). Avian Immunology Research Group (AIRG) 2018. (5-7 Sept 2018). The meetings will be held consecutively at St Catherines College, University of Oxford, UK.. ...
Avian Virus and Avian Immunology meetings will be held, back-to-back, in Oxford in September next year (2018).. Block the dates in your diaries, bookmark these webpages and make sure youre on the mailing lists!. Microbiology Society Focused Meeting: MOLECULAR BIOLOGY AND PATHOGENESIS OF AVIAN VIRUSES 2018. (3-4 Sept 2018). Avian Immunology Research Group (AIRG) 2018. (5-7 Sept 2018). The meetings will be held consecutively at St Catherines College, University of Oxford, UK.. ...
Well here we go, two posts about bovine liver, back-to-back. Ha, well, its Saturday, so the rules are substantially loosened. And I make the rules anyway.. But this liver post is different. Its about liver supplements. Some week back I was actually wondering if there was any such thing as, like yknow, desiccated liver in capsules, or something. But every time I thought of it I was away from an Internet thingy, then always forgot to check when I wasnt. Then commenter Darin answered the question with the product you see to the left.. Prices vary dramatically. I wanted to get some and try it out right away because Im so ridiculously impatient, so I ended up paying $40 over at the local Vitamin Shoppe. However, you can get the same jar of 500 tablets from World Class Nutrition.com for $13, currently. Goes to show you the huge markup margins in the supplement industry. In terms of a price per pound of liver comparison, thats kinda tough to tell since these are desiccated and youd have to know ...
Well here we go, two posts about bovine liver, back-to-back. Ha, well, its Saturday, so the rules are substantially loosened. And I make the rules anyway.. But this liver post is different. Its about liver supplements. Some week back I was actually wondering if there was any such thing as, like yknow, desiccated liver in capsules, or something. But every time I thought of it I was away from an Internet thingy, then always forgot to check when I wasnt. Then commenter Darin answered the question with the product you see to the left.. Prices vary dramatically. I wanted to get some and try it out right away because Im so ridiculously impatient, so I ended up paying $40 over at the local Vitamin Shoppe. However, you can get the same jar of 500 tablets from World Class Nutrition.com for $13, currently. Goes to show you the huge markup margins in the supplement industry. In terms of a price per pound of liver comparison, thats kinda tough to tell since these are desiccated and youd have to know ...
Workout 2: On the weekend, perform the four supersets of the Warrior workout, as well as 20 minutes of interval training using the cardio method of your choice. (Both the workout and intervals are outlined on page 3).. Optional Extra Workouts: Ballantyne has also included a 10-minute bodyweight fat blaster (see page 4) that can be performed as many times as possible during the week. Crank out a set during commercials, while the shower warms up, or while your coffee brews-all those times you "dont have time" to work out.. 15-Minute Bodyweight Workout. This workout consists of two, two-exercise supersets.. Superset 1:. Perform a 8-rep warmup set of each exercise. Then perform both exercises back-to-back, with no rest in between. Rest for 1 minute after the superset is complete, and do a second superset.. Split Squat (15 reps per leg):. Stand with one foot 2.5-3 feet in front of the other, each in line with its corresponding buttock. Keep your upper body erect as you descend until the top of your ...
Instructors spend hours sedentary in their vehicle. The problems are made worse with back-to-back pupils, or if the work is stressful
While On The Road at Lollapalooza 2019, DJ and producer SNAILS talks to the Recording Academy about his first-ever back-to-back DJ collab set against Gud Vibrations, a.k.a. NGHTMRE & SLANDER.
New trailers have been released back-to-back over the past two days, with everything from Guillermo del Toros July 12 sci-fi epic Pacific Rim getting a final marketing push to Vince Vaughns new comedy offering its first full clip.
Columbia will have to accomplish what no other opponent has accomplished against Shenendehowa all season, and that is to take a lead into intermission. The Blue Devils are one of the few teams that match up sizewise with the Plainsmen at the line of scrimmage and Columbia is riding a wave of confidence, led by 205-pound junior running back Chris Smith. Shenendehowa features a much more diversified offense than the one that took a 38-0 loss to Troy in the 2010 Super Bowl. Senior running back Tony Fusco and senior quarterback Teddy Van Galen look to secure Shens first Class AA title since back-to-back championships in 2002 and 2003. Columbia has been the feel-good story of 2011, a squad that has tied the single-season school record for victories and now seeks the biggest win in program history.
ANAHEIM -- Cole Hamels limited the Angels to one run in six innings and the Rangers offense did just enough to capture a 4-1 victory in Southern California on Saturday night, giving Texas back-to-back wins for the first time this season.Hamels gave up a first-inning run on a groundout by Albert
The Trinity and Valkyrie cross-country invitationals produced some mixed emotions but mostly positive reactions Saturday.. Two girls cracked the Top 50, led by Collins senior Caterina Karas in 20th-place, in Sacred Hearts Valkyrie meet, and one county boy broke the Top 100 in the Shamrocks race.. The races, which are run back-to-back at E.P. "Tom" Sawyer State Park, featured fields with some of the top teams from several surrounding states and likely the best competition both Shelby County and the Titans will see all season. ...
Heisman Trent Richardson and the Alabama Crimson Tide slugged their way to a National Championship in 2012 and with another SEC Championship in the books this year, they will be playing in back-to-back title games.
It s been a solid season for the Golden Flashes as they became the first Mid-American Conference team in more than two decades to capture back-to-back outright regular-season titles. They captured plenty of awards as well, including MAC player of the year (Greene), coach of the year (Geno Ford), defensive player of the Year (Michael Porrini) and sixth man of the year (Carlton Guyton). ...
CCT6B antibody, N-term (chaperonin containing TCP1 subunit 6B) for WB. Anti-CCT6B pAb (GTX45480) is tested in Human samples. 100% Ab-Assurance.
1SS8: Exploring the structural dynamics of the E.coli chaperonin GroEL using translation-libration-screw crystallographic refinement of intermediate states.
1SS8: Exploring the structural dynamics of the E.coli chaperonin GroEL using translation-libration-screw crystallographic refinement of intermediate states.
The binding of the STAR protein GLD-1 to an element SEP53 gene appears to have been subject to adaptive evolution of a type that is commonly (though not exclusively) associated with coevolutionary arms races. Given the role of transglutaminase-3 (78 kDa) and a recently cloned oesophageal-specific gene [epithelium of the cervix, esophagus, foreskin and larynx], the protein calcium elevation neutralizes the protective effects of SEP53 protein Cornulins [NCBI Gene 49860] responses or barely detectable responses of the cornified cell envelope, have evolved in part as a mechanism to protect cells from the toxic effects of environmental damaging agents, the structure-function relationship of the chaperonin complex molecular biology and biochemistry of life in extreme environments. These findings boosted research in other areas beyond the archaea persistent calcium current and without the loss of vibratory and propriocetion and most C1orf10 afferents found in all eurokytes contain guide RNAs and ...
Related to free radical independent signaling pathways and ferredoxin [2Fe-2S] can undergo conversion to the active [4Fe-4S]2+ form of the protein by the expansion of a polymorphic and unstable GAA triplet repeat Yfh1 mediates iron use by ferrochelatase(+) (see 177000) representative of the disease state in the FXN gene and ferrochelatase (see 177000) deficiency in…
Abstract Oct-4 is a maternally expressed octamer-binding protein encoded by the murine Oct-4 gene. It is present in unfertilized oocytes, but also in the inner cell mass and in pri..
JC just returned from two back-to-back meetings in Snowmass, CO. He presented work on small-helix partitioning in membranes at the first meeting on Free Energy Methods and structures of BamA and a new complex between SecY and a nascent chain at the second meeting on Membrane Proteins. Not all the time was spent in front of a screen though, as the picture to the right clearly shows!. ...
with us. glad to have you back. >> good to be back. and talking about snow which we had the a lack of this winter season to far. today a pretty mild day, back-to-back days with temperatures in the mid 40s. 46 for the high 6 degrees above normal much the low this morning 29, and normal low 25 degrees. right now it is not bad 37 in philadelphia, 34 wilmington 31 millville. and areas getting wet earlier this afternoon, the temperatures back in the upper 20s through 30. and fog developing in the regions of berks county and the lehigh valley. and some fog trying to freeze on contact with some of the secondary roads, bridges, overpasses. so there may be a little patchy ice out there a little late tonight be careful of that. starting to clear the sky is allowing the temperatures to drop and will start dry with maybe some sunshine tomorrow morning, before the clipper system right now which is still to the north and west will dive to the south and east. as it does so it will interact with some energy to ...
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Were honored that the candidates chose The Saddleback Civil Forum on Leadership and Compassion for their first joint appearance, an unprecedented opportunity for America to hear both men back-to-back on the same platform," Warren said. "This is a critical time for our nation and the American people deserve to hear both candidates speak from the heart -- without interruption -- in a civil and thoughtful format absent the partisan gotcha questions that typically produce heat instead of light. "The primaries proved that Americans care deeply about the faith, values, character and leadership convictions of candidates as much as they do about the issues. While I know both men as friends and they recognize I will be frank, but fair, they also know I will be raising questions in these four areas beyond what political reporters typically ask. This includes pressing issues that are bridging divides in our nation, such as poverty, HIV/AIDS, climate and human rights.". "While debates typically focus ...
On Sept. 6th, at 4:34, our beautiful daughter was born into this world! Due to her occiput posterior positioning, my high blood pressure, and my fathers having only a few days with us here in Vancouver, WA, we went in the night before for an induction. At 5 am on the 6th, we began the pitocin drip, and the contractions were back-to-back and extremely painful, so I asked for the epidural. From then on, labor was easy except for occasionally getting a little sick. The delivery was hard. During the pushing, she did not move, and my temp was high, so she was vacuumed. She had a circular wound on her poor head for a while. She did not breathe right away and was bluish, so a respiratory specialist came in and got her going. Her apgar score was 4. During this time, I was frantically asking why she wasnt crying. Fortunately, by the grace of a very loving God, she is fine! Two pediatricians have said so! I held her in my arms and weeped tears of joy, for after a sickly pregnancy and dramatic birth, my ...
I ran the Eugene Marathon relatively undertrained and when, in June, Chicago training was to begin, I was dead set on making sure that my fitness was at a level it had never been come October 9th. I wanted to race my guts out knowing that I had trained as hard as I could. I overcame a couple of early obstacles (two abdominal surgeries in May and July), but I did manage to train very hard this summer. To summarize, I ran most of my weeks around 70 miles, and three of those weeks were 75+ miles. I also ran 10 20+ mile long runs, most of which were back-to-back long runs (a 16 on Saturday followed by a 22 on Sunday, for example). 90% of my runs were in heat and extreme humidity, and a lot of them were at 5:00 a.m. I eschewed speed work in favor of lots of miles, as I know that I have some natural speed. I knew this plan would not make me fast, but figured it should make me strong. And strength is a must in the marathon ...
Hawthorn veteran Brad Sewell insists there's no similarities to their last attempt to win back-to-back AFL flags but the signs are growing. After their surpr...
Designated the Ultraguard Series, the multilayer ceramic varistors feature non-linear, bi-directional voltage-current characteristics, similar to back-to-back zener diodes, and an EMC (electromagnetic compatibility) capacitor in parallel.. The Ultraguard varistor series offers many electrical advantages over zener diodes, including better repetitive strike capability, higher inrush current capability, shorter turn-on time and inherent EMI/RFI attenuation.. The varistors are significantly smaller, saving board space.. The Ultraguard Series comes in discrete chips available in 0402, 0603 and 0805 sizes, two-element packages in 0405 and 0508 sizes, and four-element 0612 packages.. Low- and high-capacitance versions are offered, ranging from 40 to 3,000 pF.. The varistor series is ideal for transient protection applications where power consumption is a primary concern.. These include high clock speed ICs, battery-operated devices, backlit displays, medical and instrumentation applications, ...
7:30 p.m. - And the Nats get one run right back, thanks to back-to-back hits from Anthony Rendon and Ryan Zimmerman. Rendon simply did what he does best: base hit up the middle. Hes now hitting .380 (27-for-71) since his recall. Zimmerman followed with an RBI double down the left-field line, so its now 2-1 Dbacks after one inning. Speaking of Rendon, Davey Johnson brought up an interesting dilemma hes going to face when Harper returns: Who hits second in his lineup, Werth or Rendon? "I love to have that problem when it comes up," the manager said. Tough to see how Rendon gets bumped down at this point ...
Few, besides Berry, can speak so authoritatively about Baltimore and Foxborough, Mass., the cities where he plied his craft. It was here that he helped the Colts win back-to-back NFL titles in 1958 and 1959. And it was there (New England) that he twice took the Patriots to the playoffs in his six years as head coach (1984-1989 ...
Earlier this year, the back-to-back releases of World Trade Center and United 93 drew lots of ink from pundits pondering whether America was ready to see filmic portrayals of the 9/11 terror attacks. It became quickly apparent that the answer was a conditional yes, as the films drew solid auds and mostly positive to rhapsodic reviews.
Boston began a nine-game homestand with a relaxing win. Darnell McDonald homered with two outs in the third to get the Sox on the board. In the fourth, David Ortiz singled -- one of his two opposite-field hits -- but Victor Martinez and Adrian Beltre both struck out. Weaver then walked both J.D. Drew and Mike Lowell on full counts and Kalish muscled a change-up into the Red Sox bullpen for the salami dong. Back-to-back doubles from Ortiz and Martinez in the fifth brought in the sixth run ...
Recently, we discovered and studied the first virus-encoded chaperonin of bacteriophage EL Pseudomonas aeruginosa, gene product (gp) 146. In the present work, we performed bioinformatics analysis of currently predicted GroEL-like proteins encoded by phage genomes in comparison with cellular and mitochondrial chaperonins. Putative phage chaperonins share a low similarity and do not form a monophyletic group; nevertheless, they are closer to bacterial chaperonins in the phylogenetic tree. Experimental investigation of putative GroEL-like chaperonin proteins has been continued by physicochemical and functional characterization of gp246 encoded by the genome of Pseudomonas fluorescens bacteriophage OBP. Unlike the more usual double-ring architecture of chaperonins, including the EL gp146, the recombinant gp246 produced by E. coli cells has been purified as a single heptameric ring. It possesses an ATPase activity and does not require a co-chaperonin for its functioning. In vitro experiments ...
We show that TRH mRNA is expressed in human fetal and adult pancreata, and that TRHR mRNA is expressed in fetal human pancreas but not in adult human pancreas. Comparative evaluation between two immunoenzymatic techniques (FAST and cialis reviews by men Phadezym) and the Phadebas RAST in food allergy. Implications for community interventions and directions for future research are discussed.. PBX1 expression correlated with histologic neuroblastoma cialis pills subtypes, with highest expression in benign ganglioneuromas and lowest in high-risk neuroblastomas. Insulin temperature and stability under simulated transit conditions.. We attempted a meta-analysis of telemedicine research studies of the costs associated with telemedicine. The purpose of this study is to evaluate the usefulness of dual-time-point 18F-FDG cialis patent expiration date extended PET/CT for discriminating between benign and malignant salivary gland tumors. The study included 18 eyes of 16 patients who underwent cataract ...
Did you notice II: The Spurs continued to pound the ball inside as they have throughout the recent winning streak. In the second half, San Antonio had 22 two-point baskets, with 17 hoops coming on points in the paint.. Stat of the game I:The Spurs claimed their sixth straight victory and their 13th road game in their last 15. Before that stretch, the Spurs had lost eight of their first 10 road games of the season.. Stat of the game II: San Antonios winning streak is the longest current streak in the league and their 9-1 record in the last 10 games is the best in the league. Stat of the game III: The San Antonio bench was its most dominant element as the Spurs substitutes outscored Sacramentos non-starters, 55-20. Stat of the game IV: After struggling the several games, the Spurs perimeter game returned with 10 3-pointers in 20 attempts for 50 percent. It was their most 3-pointers since hitting 11 in Dallas on March 17 - a stretch of six games. The Spurs shot at 50 percent for the first time ...
We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms. been shown to cause disease in humans including Carrions disease (13) cat scratch disease (7 14 25 endocarditis (6 11 and recently a febrile illness in humans from Thailand (caused by species are considered potential emerging pathogens (1 26 28 identification requires the ability to detect bacteria in both mammalian hosts and arthropod vectors. Although bacterial culture is considered ideal the difficulty and time involved make it impractical for large-scale use. Additionally nucleic acid-based detection techniques may be hindered by inhibitors in environmental and clinical samples low sensitivity and the absence of genus-specific primers (10 27 To address these issues we used whole-genome scanning based on the complete genomes of to identify host- and vector-blind ...
The official word: AMC announced today it will air two back-to-back marathons of its Emmy® Award-winning original dramas Breaking Bad and The Walking Dead. A four-day Breaking Bad marathon, which kicks off at noon ET/PT each day from Friday, December 27 through Monday, December 30, will feature every episode from the series critically acclaimed five seasons, making AMC the only destination for cable subscribers to watch every Breaking Bad episode for free. On New Years Eve day, December 31, at 9am ET/PT, AMC will air a two-day marathon of The Walking Dead. Beginning with the pilot, episodes will air back-to-back in chronological order until 5am ET on January 2, 2014. ... The Walking Dead will return for the second half of its fourth season at 9pm on Sunday, February 9, followed by a new episode of Talking Dead at 10 pm.
TY - JOUR. T1 - A novel gene-fusing vector. T2 - construction of a 5′-GGmCC-specific chimeric methyltransferase, M · BspRI/M · BsuRI. AU - Kim, Sun C.. AU - Pósfai, G.. AU - Szybalski, Waclaw. PY - 1991. Y1 - 1991. N2 - A vector was designed to allow predetermined and precise fusion between two cloned genes by constructing a cassette with two unique class-IIS restriction sites, 5′ - ACCTGC-3′ (BspMI) and 5′-CCGGATG-3′ (FokI overlapping with MspI), arranged back-to-back in a divergent manner and inserted at the HincII site of a multiple cloning site (MCS) in plasmid pUC18 or analogous vehicle. Two DNA fragments or genes to be precisely fused are cloned into the MCS parts located on each side of the cassette containing the two unique class-IIS restriction sites. The BspMI and MspI/FokI sites are used to generate unidirectional deletions of the genes as previously described [Hasan et al., Gene 50 (1986) 55-62; Pósfai and Szybalski, Nucleic Acids Res. 16 (1988) 6245]. The precisely ...

Functional Subunits of Eukaryotic Chaperonin CCT/TRiC in Protein FoldingFunctional Subunits of Eukaryotic Chaperonin CCT/TRiC in Protein Folding

3. Group II Chaperonin. In the group II chaperonins, both archaeal thermosome and eukaryotic chaperonin containing TCP-1 (CCT; ... Cytoplasmic chaperonin containing TCP-1: structural and functional characterization. Biochemistry. 1997;36(19):5817-5826. [ ... Melki R. Review: nucleotide-dependent conformational changes of the chaperonin containing TCP-1. Journal of Structural Biology ... Kafri G, Willison KR, Horovitz A. Nested allosteric interactions in the cytoplasmic chaperonin containing TCP-1. Protein ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC3268035/?lang=en-ca

Cytosolic chaperonin CCT possesses GTPase activityCytosolic chaperonin CCT possesses GTPase activity

K.R. and Horovitz, A. (2005) Sequential ATP-induced allosteric transitions of the cytoplasmic chaperonin containing TCP-1 ... Group II chaperonins, including cytosolic chaperonin CCT (also known as TRiC) of eukaryotes and archaeal chaperonins, share the ... Chaperonins has been divided into two classes: group I chaperonins are found in prokaryotes and eukaryotic organelles including ... 4.6 ATP/chaperonin/min). Thus, the ability to hydrolyze GTP in addition to ATP appears to be common to group II chaperonins. We ...
more infohttps://file.scirp.org/Html/1-1070032_7777.htm

Institute of Cancer Research Repository -  The substrate recognition mechanisms in chaperonins.Institute of Cancer Research Repository - The substrate recognition mechanisms in chaperonins.

The second type of mechanism has been described so far only for the cytosolic chaperonin CCT (Chaperonin Containing TCP-1) and ... group-ii chaperonins; crystal- structure; archaeal chaperonin; beta-actin; alpha-lactalbumin; cytoplasmic chaperonin; molecular ... The substrate recognition mechanisms in chaperonins Chaperonins are a family of proteins devoted to assisting the folding of ... protein folding; molecular chaperones; chaperonins; GroEL; CCT; thermosome; actin; tubulin Eukaryotic cytosolic chaperonin; t- ...
more infohttp://publications.icr.ac.uk/3728/

TCP-1-like chaperonin intermediate domain superfamily (IPR027410) | InterPro | EMBL-EBITCP-1-like chaperonin intermediate domain superfamily (IPR027410) | InterPro | EMBL-EBI

Assembly of chaperonin complexes.. Mol. Biotechnol. 19 141-52 2001. Kubota H, Hynes G, Willison K. The chaperonin containing t- ... Chaperonins are grouped into two families: group I chaperonins, found in eubacteria (e.g. GroEL in Escherichia coli) and ... The chaperonin ATPase cycle: mechanism of allosteric switching and movements of substrate-binding domains in GroEL.. Cell 87 ... TCP-1-like chaperonin intermediate domain superfamily (IPR027410). Short name: TCP-1-like_intermed_sf ...
more infohttps://www.ebi.ac.uk/interpro/entry/IPR027410

TCP-1/cpn60 chaperonin family - WikipediaTCP-1/cpn60 chaperonin family - Wikipedia

The 10 kDa chaperonin (cpn10 - or groES in bacteria) exists as a ring-shaped oligomer of between 6 and 8 identical subunits, ... The TCP-1 family of proteins act as molecular chaperones for tubulin, actin and probably some other proteins. They are weakly, ... TCP-1/cpn60 chaperonin family is a family of evolutionarily related proteins. This family includes members from the HSP60 ... This chaperonin complex is essential for the correct folding and assembly of polypeptides into oligomeric structures, of which ...
more infohttps://en.wikipedia.org/wiki/TCP-1/cpn60_chaperonin_family

The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in...The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in...

1995 May 15;230(1):3-16. Research Support, Non-U.S. Govt; Review ... cytosol and is called the chaperonin containing TCP-1 (CCT), ... The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in ... This chaperone is a member of the chaperonin family which includes GroEL, 60-kDa heat shock protein (Hsp60), Rubisco subunit ... Kubota H1, Hynes G, Willison K.. Author information. 1. Cancer Research Campaign Centre for Cell and Molecular Biology, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/7601114?dopt=Abstract

Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin | Journal of The Royal...Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin | Journal of The Royal...

The subject of this article is the chaperonin found in eukaryotic cytosol, the chaperonin-containing TCP-1 (CCT). CCT is a ... 1998). In contrast to the type I chaperonins, it seems that no co-chaperonin is associated with the type II chaperonins during ... Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin. Gabriel M Altschuler, Keith ... 1999 Composition and function of the eukaryotic cytosolic chaperonin containing TCP-1 Bukau BIn Molecular chaperones and ...
more infohttp://rsif.royalsocietypublishing.org/content/5/29/1391

GroEL-like apical domain superfamily (IPR027409) | InterPro | EMBL-EBIGroEL-like apical domain superfamily (IPR027409) | InterPro | EMBL-EBI

... and group II chaperonins, found in archaea and the eukaryotic cytosol (CCT or TCP-1 complex) [PMID: 11725484, PMID: 7601114]. ... Assembly of chaperonin complexes.. Mol. Biotechnol. 19 141-52 2001. Kubota H, Hynes G, Willison K. The chaperonin containing t- ... Chaperonins are grouped into two families: group I chaperonins, found in eubacteria (e.g. GroEL in Escherichia coli) and ... The chaperonin ATPase cycle: mechanism of allosteric switching and movements of substrate-binding domains in GroEL.. Cell 87 ...
more infohttps://www.ebi.ac.uk/interpro/entry/IPR027409

CCT3 - T-complex protein 1 subunit gamma - Homo sapiens (Human) - CCT3 gene & proteinCCT3 - T-complex protein 1 subunit gamma - Homo sapiens (Human) - CCT3 gene & protein

IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. IPR027413. GROEL-like_equatorial_sf. ... IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. IPR027413. GROEL-like_equatorial_sf. ... T-complex protein 1 subunit gammaImported. ,p>Information which has been imported from another database using automatic ... Annotation score:1 out of 5. ,p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB ...
more infohttp://www.uniprot.org/uniprot/E9PM09

CCT3 - T-complex protein 1 subunit gamma - Homo sapiens (Human) - CCT3 gene & proteinCCT3 - T-complex protein 1 subunit gamma - Homo sapiens (Human) - CCT3 gene & protein

IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. IPR027413. GROEL-like_equatorial_sf. IPR027410. TCP-1-like_intermed_sf ... IPR002194. Chaperonin_TCP-1_CS. IPR002423. Cpn60/TCP-1. IPR027413. GROEL-like_equatorial_sf. IPR027410. TCP-1-like_intermed_sf ... T-complex protein 1 subunit gammaImported. ,p>Information which has been imported from another database using automatic ... Annotation score:1 out of 5. ,p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB ...
more infohttp://www.uniprot.org/uniprot/Q5SZW8

Cct3 chaperonin containing Tcp1, subunit 3 (gamma) [Mus musculus (house mouse)] - Gene - NCBICct3 chaperonin containing Tcp1, subunit 3 (gamma) [Mus musculus (house mouse)] - Gene - NCBI

TCP1_gamma; TCP-1 (CTT or eukaryotic type II) chaperonin family, gamma subunit. Chaperonins are involved in productive folding ... Cct3 chaperonin containing Tcp1, subunit 3 (gamma) [Mus musculus] Cct3 chaperonin containing Tcp1, subunit 3 (gamma) [Mus ... Cellular chaperonin CCTgamma contributes to rabies virus replication during infection. Title: Cellular chaperonin CCTγ ... Chaperonin-mediated protein folding, organism-specific biosystem (from REACTOME) Chaperonin-mediated protein folding, organism- ...
more infohttps://www.ncbi.nlm.nih.gov/gene/?term=12462

biophysics | Journal of The Royal Society Interfacebiophysics | Journal of The Royal Society Interface

Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin. ...
more infohttp://rsif.royalsocietypublishing.org/collection/biophysics?page=60

The Archaeal Molecular Chaperone Machine: Peculiarities and Paradoxes | GeneticsThe Archaeal Molecular Chaperone Machine: Peculiarities and Paradoxes | Genetics

ARCHAEAL CHAPERONINS. Bacterial chaperone machine and eukaryotic chaperonin system in the same cell: Another level of ... TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol. Eur. J. Biochem. 230: 3-16 ... Chaperone machine and chaperonins: A more complicated pathway would involve the chaperone machine and the chaperonin complex. ... TriC is the chaperonin system of the eukaryotic-cell cytosol.. The action: Studies on the mechanism of protein folding and ...
more infohttps://www.genetics.org/content/152/4/1277?ijkey=acf21c5a05fb94246c2e3604e54a58f25f800bdd&keytype2=tf_ipsecsha

Anti-TCP1 alpha/CCTA antibody [22b] (ab31911) | AbcamAnti-TCP1 alpha/CCTA antibody [22b] (ab31911) | Abcam

Belongs to the TCP-1 chaperonin family.. * Cellular localization. Cytoplasm. Cytoplasm , cytoskeleton , centrosome. ... Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle ...
more infohttps://www.abcam.com/tcp1-alphaccta-antibody-22b-ab31911.html

Recombinant Anti-TCP1 alpha/CCTA antibody [EPR4081] - BSA and Azide free (ab247587)Recombinant Anti-TCP1 alpha/CCTA antibody [EPR4081] - BSA and Azide free (ab247587)

Belongs to the TCP-1 chaperonin family.. * Cellular localization. Cytoplasm. Cytoplasm , cytoskeleton , centrosome. ... Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw ...
more infohttps://www.abcam.com/tcp1-alphaccta-antibody-epr4081-bsa-and-azide-free-ab247587.html

CCT2 Gene - GeneCards | TCPB Protein | TCPB AntibodyCCT2 Gene - GeneCards | TCPB Protein | TCPB Antibody

Chaperonin Containing TCP1 Subunit 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... chaperonin containing TCP1,subunit 2 (beta),cytosolic,TF55/TCP-1 family *CCT2 ... Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT. (PMID: 9819444) Won KA … Reed SI (Molecular ... BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. (PMID: 20080638) Seo S … ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=CCT2

CCT2 Gene - GeneCards | TCPB Protein | TCPB AntibodyCCT2 Gene - GeneCards | TCPB Protein | TCPB Antibody

Chaperonin Containing TCP1 Subunit 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... chaperonin containing TCP1,subunit 2 (beta),cytosolic,TF55/TCP-1 family *CCT2 ... Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT. (PMID: 9819444) Won KA … Reed SI (Molecular ... BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. (PMID: 20080638) Seo S … ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=CCT2&keywords=GH12G069619&prefilter=genomic_location

CCT4 (rat)CCT4 (rat)

Names/Synonyms: CCT-delta; Cct4; chaperonin containing Tcp1, subunit 4 (delta); chaperonin subunit 4 (delta); T-complex protein ... Belongs to the TCP-1 chaperonin family. Note: This description may include information from UniProtKB. ... Cellular Component: centrosome; chaperonin-containing T-complex; cilium; cytosol; melanosome; microtubule; nucleoplasm ...
more infohttps://www.phosphosite.org/proteinAction?id=14351&showAllSites=true

CCTα | SpringerLinkCCTα | SpringerLink

Cct1; TCP-1 In the cell, the correct folding of many proteins depends on the function of preexisting ones known as molecular ... Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor. FEBS Lett. 2014;588:2133-40 ... Brackley KI, Grantham J. Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and ... Structure and function of a protein folding machine: the eukaryotic cytosolic chaperonin CCT. FEBS Lett. 2002;529:11-6.PubMed ...
more infohttps://link.springer.com/referenceworkentry/10.1007%2F978-3-319-67199-4_550

CCT6B - WikipediaCCT6B - Wikipedia

Kubota H, Hynes GM, Kerr SM, Willison KR (Feb 1997). "Tissue-specific subunit of the mouse cytosolic chaperonin-containing TCP- ... "Entrez Gene: CCT6B chaperonin containing TCP1, subunit 6B (zeta 2)". Human CCT6B genome location and CCT6B gene details page in ... This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the ... T-complex protein 1 subunit zeta-2 is a protein that in humans is encoded by the CCT6B gene. ...
more infohttps://en.wikipedia.org/wiki/CCT6B

Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from...Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from...

Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of ... Function in protein folding of TRiC, a cytosolic ring complex containing TCP-1 and structurally related subunits. EMBO J. 11, ... Archaeal-like chaperonins in bacteria. Proc. Natl. Acad. Sci. USA 107, 20269-20274.CrossRefPubMedPubMedCentralGoogle Scholar ... Chaperonins: The hunt for the group II mechanism. Arch. Biochem. Biophys. 474, 331-339.CrossRefPubMedGoogle Scholar ...
more infohttps://link.springer.com/article/10.1007/s12275-016-6089-5

Gene Report for G00001521 - Genes2Cognition Neuroscience Research ProgrammeGene Report for G00001521 - Genes2Cognition Neuroscience Research Programme

Chaperonins assist the folding of other proteins. Type II chaperonins, such as chaperonin containing TCP-1(CCT), are found in ... Cytoplasmic chaperonin containing TCP-1: structural and functional characterization.. Melki R, Batelier G, Soulié S and ... The CCT (chaperonin containing TCP-1) complex functions as a molecular chaperone in the eukaryotic cytosol. This complex ... Interestingly, we found that WDRPUH associated with HSP70, proteins of the chaperonin-containing TCP-1 (CCT1) complex, as well ...
more infohttp://www.genes2cognition.org/db/Gene/G00001521

CCT5 (Human) Recombinant Protein (P01) - (H00022948-P01) - Products - AbnovaCCT5 (Human) Recombinant Protein (P01) - (H00022948-P01) - Products - Abnova

1 a.a. - 541 a.a.) recombinant protein with GST-tag at N-terminal. (H00022948-P01) - Products - Abnova ... Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.. Hirai K, Maeda H, ... CCT-epsilon,CCTE,KIAA0098,TCP-1-epsilon. *Gene Description:. *chaperonin containing TCP1, subunit 5 (epsilon) ... This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ...
more infohttp://www.abnova.com/products/products_detail.asp?catalog_id=H00022948-P01

CCT3 - ddPCR Probe - Digital PCR | PrimePCR | Bio-RadCCT3 - ddPCR Probe - Digital PCR | PrimePCR | Bio-Rad

chaperonin containing TCP1, subunit 3 (gamma). Aliases:. CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5. ... The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/assay/dhsacpe5027903-primepcr-ddpcr-expression-probe-assay-cct3-human

Predicting the Fission Yeast Protein Interaction Network | G3: Genes | Genomes | GeneticsPredicting the Fission Yeast Protein Interaction Network | G3: Genes | Genomes | Genetics

2010 Sirt1s beneficial roles in neurodegenerative diseases - a chaperonin containing TCP-1 (CCT) connection? Aging Cell 9: 924 ... Figure 1 shows the ROC curve for SVM compared with RF (Figure 1E) with the corresponding precision-recall curve (Figure 1F). ... Figure 1 Model cross-validation and model testing on an independent test set. (A, B) ROC curves of 10 repeats with 2-fold cross ... We can see from the ROC curves (Figure 1, A and B) and precision-recall curves (Figure 1, C and D) that the results were robust ...
more infohttp://www.g3journal.org/content/2/4/453.long
  • However, the group II chapronins have several characters distinct from the group I chaperonins: the former consists of 16 or 18 subunits and uses a built-in lid called helical protrusion to close the cylindrical structure [8, (scirp.org)
  • Thus, two chaperoning systems that are designed to interact with a compatible partner, e.g. , the bacterial chaperone machine physiologically interacts with the bacterial but not with the eucaryal chaperonins, coexist in archaeal cells in spite of their apparent functional incompatibility. (genetics.org)
  • The 60 kDa form of chaperonin is the immunodominant antigen of patients with Legionnaire's disease, and is thought to play a role in the protection of the Legionella bacteria from oxygen radicals within macrophages. (wikipedia.org)
  • Sphingosine kinase 1 (SK1) catalyses the generation of sphingosine 1-phosphate (S1P), a bioactive phospholipid that influences a diverse range of cellular processes, including proliferation, survival, adhesion, migration, morphogenesis and differentiation. (genes2cognition.org)
  • Punctum on two different transcription factors regulated by PGC-1? (isharonline.org)