Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE.
A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)
A class of sphingolipids found largely in the brain and other nervous tissue. They contain phosphocholine or phosphoethanolamine as their polar head group so therefore are the only sphingolipids classified as PHOSPHOLIPIDS.
Cerebrosides which contain as their polar head group a glucose moiety bound in glycosidic linkage to the hydroxyl group of ceramides. Their accumulation in tissue, due to a defect in beta-glucosidase, is the cause of Gaucher's disease.
A ceramidase subtype that is active at alkaline pH. It is found at high levels within the SMALL INTESTINE.
An amino alcohol with a long unsaturated hydrocarbon chain. Sphingosine and its derivative sphinganine are the major bases of the sphingolipids in mammals. (Dorland, 28th ed)
An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12.
Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)
An enzyme that catalyzes the acyltransferase of SPHINGOSINE to N-acylsphingosine using acyl-COENZYME A as donor and COENZYME A as acceptor. The enzyme is mainly localized in the MITOCHONDRIA.
Amidohydrolases that are specific for the cleavage of the N-acyl linkage of CERAMIDES. Ceramidases are classified as acidic, neutral or basic according to the optimal pH with which they function.
A key enzyme in SPHINGOLIPIDS biosynthesis, this enzyme catalyzes the pyridoxal-5'-phosphate-dependent condensation of L-SERINE and PALMITOYL COENZYME A to 3-dehydro-D-sphinganine. The enzyme consists of two different subunits.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)
The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
A blood group related both to the ABO and P systems that includes several different antigens found in most people on erythrocytes, in milk, and in saliva. The antibodies react only at low temperatures.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Loss of water by diffusion through the skin and by evaporation from the respiratory tract.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Conditions characterized by abnormal lipid deposition due to disturbance in lipid metabolism, such as hereditary diseases involving lysosomal enzymes required for lipid breakdown. They are classified either by the enzyme defect or by the type of lipid involved.
A group of MYCOTOXINS found in CORN contaminated with FUSARIUM fungus. They are chains of about 20 carbons with acidic ester, acetylamino and sometimes other substituents. They inhibit ceramide synthetase conversion of SPHINGOLIPIDS to CERAMIDES.
A ceramidase subtype that is active at acid pH. It plays an important role in sphingolipid degradation by catalyzing the lysosomal hydrolysis of ceramide to sphingosine and free fatty acid. Inherited deficiency of acid ceramidase activity results in FARBER LIPOGRANULOMATOSIS.
A subclass of GLYCOSPHINGOLIPIDS containing one or more sugars within their head group connected directly to a ceramide moiety. They consist of monoglycosyl-, and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
GLYCOSPHINGOLIPIDS with a sulfate group esterified to one of the sugar groups.
Organic compounds containing both the hydroxyl and carboxyl radicals.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
Compounds possessing both a hydroxyl (-OH) and an amino group (-NH2).
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
Derivatives of PHOSPHATIDIC ACIDS that lack one of its fatty acyl chains due to its hydrolytic removal.
Mutant strains of mice that produce little or no hair.
A ganglioside present in abnormally large amounts in the brain and liver due to a deficient biosynthetic enzyme, G(M3):UDP-N-acetylgalactosaminyltransferase. Deficiency of this enzyme prevents the formation of G(M2) ganglioside from G(M3) ganglioside and is the cause of an anabolic sphingolipidosis.
A glycosidase that hydrolyzes a glucosylceramide to yield free ceramide plus glucose. Deficiency of this enzyme leads to abnormally high concentrations of glucosylceramide in the brain in GAUCHER DISEASE. EC 3.2.1.45.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Cerebrosides which contain as their polar head group a galactose moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in beta-galactosidase, is the cause of galactosylceramide lipidosis or globoid cell leukodystrophy.
An enzyme that catalyzes the hydrolysis of a ceramidetrihexoside to a ceramidedihexoside plus galactose.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
A ceramidase subtype that is active at neutral pH. It is found at high levels within the SMALL INTESTINE and in the BRAIN.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.
Glycosphingolipids which contain as their polar head group a lactose moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in lactosylceramide beta-galactosidase, is the cause of lactosylceramidosis.
The rotation of linearly polarized light as it passes through various media.
A group of inherited metabolic disorders characterized by the intralysosomal accumulation of SPHINGOLIPIDS primarily in the CENTRAL NERVOUS SYSTEM and to a variable degree in the visceral organs. They are classified by the enzyme defect in the degradation pathway and the substrate accumulation (or storage). Clinical features vary in subtypes but neurodegeneration is a common sign.
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The process of cleaving a chemical compound by the addition of a molecule of water.
Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.

SDZ PSC 833, the cyclosporine A analogue and multidrug resistance modulator, activates ceramide synthesis and increases vinblastine sensitivity in drug-sensitive and drug-resistant cancer cells. (1/2471)

Resistance to chemotherapy is the major cause of cancer treatment failure. Insight into the mechanism of action of agents that modulate multidrug resistance (MDR) is instrumental for the design of more effective treatment modalities. Here we show, using KB-V-1 MDR human epidermoid carcinoma cells and [3H]palmitic acid as metabolic tracer, that the MDR modulator SDZ PSC 833 (PSC 833) activates ceramide synthesis. In a short time course experiment, ceramide was generated as early as 15 min (40% increase) after the addition of PSC 833 (5.0 microM), and by 3 h, [3H]ceramide was >3-fold that of control cells. A 24-h dose-response experiment showed that at 1.0 and 10 microM PSC 833, ceramide levels were 2.5- and 13.6-fold higher, respectively, than in untreated cells. Concomitant with the increase in cellular ceramide was a progressive decrease in cell survival, suggesting that ceramide elicited a cytotoxic response. Analysis of DNA in cells treated with PSC 833 showed oligonucleosomal DNA fragmentation, characteristic of apoptosis. The inclusion of fumonisin B1, a ceramide synthase inhibitor, blocked PSC 833-induced ceramide generation. Assessment of ceramide mass by TLC lipid charring confirmed that PSC 833 markedly enhanced ceramide synthesis, not only in KB-V-1 cells but also in wild-type KB-3-1 cells. The capacity of PSC 833 to reverse drug resistance was demonstrated with vinblastine. Whereas each agent at a concentration of 1.0 microM reduced cell survival by approximately 20%, when PSC 833 and vinblastine were coadministered, cell viability fell to zero. In parallel experiments measuring ceramide metabolism, it was shown that the PSC 833/vinblastine combination synergistically increased cellular ceramide levels. Vinblastine toxicity, also intensified by PSC 833 in wild-type KB-3-1 cells, was as well accompanied by enhanced ceramide formation. These data demonstrate that PSC 833 has mechanisms of action in addition to P-glycoprotein chemotherapy efflux pumping.  (+info)

Mesalamine blocks tumor necrosis factor growth inhibition and nuclear factor kappaB activation in mouse colonocytes. (2/2471)

BACKGROUND & AIMS: Derivatives of 5-aminosalicylic acid (mesalamine) represent a mainstay in inflammatory bowel disease therapy, yet the precise mechanism of their therapeutic action is unknown. Because tumor necrosis factor (TNF)-alpha is important in the pathogenesis of inflammatory bowel disease, we investigated the effect of mesalamine on TNF-alpha-regulated signal transduction and proliferation in intestinal epithelial cells. METHODS: Young adult mouse colon cells were studied with TNF-alpha, epidermal growth factor, or ceramide in the presence or absence of mesalamine. Proliferation was studied by hemocytometry. Mitogen-activated protein (MAP) kinase activation and IkappaBalpha expression were determined by Western blot analysis. Nuclear transcription factor kappaB (NF-kappaB) nuclear translocation was determined by confocal laser immunofluorescent microscopy. RESULTS: The antiproliferative effects of TNF-alpha were blocked by mesalamine. TNF-alpha and ceramide activation of MAP kinase were inhibited by mesalamine, whereas epidermal growth factor activation of MAP kinase was unaffected. TNF-alpha-stimulated NF-kappaB activation and nuclear translocation and the degradation of Ikappa-Balpha were blocked by mesalamine. CONCLUSIONS: Mesalamine inhibits TNF-alpha-mediated effects on intestinal epithelial cell proliferation and activation of MAP kinase and NF-kappaB. Therefore, it may function as a therapeutic agent based on its ability to disrupt critical signal transduction events in the intestinal cell necessary for perpetuation of the chronic inflammatory state.  (+info)

Ceramide induces cytochrome c release from isolated mitochondria. Importance of mitochondrial redox state. (3/2471)

In the present study we show that N-acetylsphingosine (C2-ceramide), N-hexanoylsphingosine (C6-ceramide), and, to a much lesser extent, C2-dihydroceramide induce cytochrome c (cyto c) release from isolated rat liver mitochondria. Ceramide-induced cyto c release is prevented by preincubation of mitochondria with a low concentration (40 nM) of Bcl-2. The release takes place when cyto c is oxidized but not when it is reduced. Upon cyto c loss, mitochondrial oxygen consumption, mitochondrial transmembrane potential (Delta Psi), and Ca2+ retention are diminished. Incubation with Bcl-2 prevents, and addition of cyto c reverses the alteration of these mitochondrial functions. In ATP-energized mitochondria, ceramides do not alter Delta Psi, neither when cyto c is oxidized nor when it is reduced, ruling out a nonspecific disturbance by ceramides of mitochondrial membrane integrity. Furthermore, ceramides decrease the reducibility of cyto c. We conclude that the apoptogenic properties of ceramides are in part mediated via their interaction with mitochondrial cyto c followed by its release and that the redox state of cyto c influences its detachment by ceramide from the inner mitochondrial membrane.  (+info)

Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells. (4/2471)

LY-A strain is a Chinese hamster ovary cell mutant resistant to sphingomyelin (SM)-directed cytolysin and has a defect in de novo SM synthesis. Metabolic labeling experiments with radioactive serine, sphingosine, and choline showed that LY-A cells were defective in synthesis of SM from these precursors, but not syntheses of ceramide (Cer), glycosphingolipids, or phosphatidylcholine, indicating a specific defect in the conversion of Cer to SM in LY-A cells. In vitro experiments showed that the specific defect of SM formation in LY-A cells was not due to alterations in enzymatic activities responsible for SM synthesis or degradation. When cells were treated with brefeldin A, which causes fusion of the Golgi apparatus with the endoplasmic reticulum (ER), de novo SM synthesis in LY-A cells was restored to the wild-type level. Pulse-chase experiments with a fluorescent Cer analogue, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-pentanoyl)-D-erythro-sphingosine (C5-DMB-Cer), revealed that in wild-type cells C5-DMB-Cer was redistributed from intracellular membranes to the Golgi apparatus in an intracellular ATP-dependent manner, and that LY-A cells were defective in the energy-dependent redistribution of C5-DMB-Cer. Under ATP-depleted conditions, conversion of C5-DMB-Cer to C5-DMB-SM and of [3H]sphingosine to [3H]SM in wild-type cells decreased to the levels in LY-A cells, which were not affected by ATP depletion. ER-to-Golgi apparatus trafficking of glycosylphosphatidylinositol-anchored or membrane-spanning proteins in LY-A cells appeared to be normal. These results indicate that the predominant pathway of ER-to-Golgi apparatus trafficking of Cer for de novo SM synthesis is ATP dependent and that this pathway is almost completely impaired in LY-A cells. In addition, the specific defect of SM synthesis in LY-A cells suggests different pathways of Cer transport for glycosphingolipids versus SM synthesis.  (+info)

Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells. (5/2471)

Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells.  (+info)

Signal transduction triggered by lipid A-like molecules in 70Z/3 pre-B lymphocyte tumor cells. (6/2471)

The lipid A (endotoxin) moiety of lipopolysaccharide (LPS) elicits rapid cellular responses from many cell types, including macrophages, lymphocytes, and monocytes. In CD14 transfected 70Z/3 pre-B lymphocyte tumor cells, these responses include activation of the MAP kinase homolog, p38, activation of NF-kappaB, and transcription of kappa light chains, leading to the assembly of surface IgM. In this work, we explored the specificity of the response with regard to lipid structure, and the requirement for p38 kinase activity prior to NF-kappaB activation in control and CD14 transfected 70Z/3 (CD14-70Z/3) cells. A p38-specific inhibitor, SB203580, was used to block p38 kinase activity in cells. CD14-70Z/3 cells were incubated with 1-50 microM SB203580, and then stimulated with LPS. Nuclear extracts were prepared, and NF-kappaB activation was measured using an electrophoretic mobility shift assay. SB203580 did not inhibit LPS induced NF-kappaB activation. In addition, LPS failed to activate p38 tyrosine phosphorylation in 70Z/3 cells lacking CD14, in spite of rapid NF-kappaB activation and robust surface IgM production with appropriate higher doses of LPS. LPS stimulation of p38 phosphorylation, NF-kappaB activation, and surface IgM expression were all blocked completely by lipid A-like endotoxin antagonists whether or not CD14 was present. Acidic glycerophospholipids and ceramides did not mimic lipid A-like molecules either as agonists or antagonists in this system. Our data support the hypothesis that lipid A-mediated activation of cells requires stimulation of a putative lipid A sensor that is downstream of CD14, but upstream of p38 and NF-kappaB.  (+info)

Nitric oxide donors induce stress signaling via ceramide formation in rat renal mesangial cells. (7/2471)

Exogenous NO is able to trigger apoptosis of renal mesangial cells, and thus may contribute to acute lytic phases as well as to resolution of glomerulonephritis. However, the mechanism involved in these events is still unclear. We report here that chronic exposure of renal mesangial cells for 24 h to compounds releasing NO, including spermine-NO, (Z)-1-{N-methyl-N-[6-(N-methylammoniohexyl)amino]}diazen-1-ium-1, 2-diolate (MAHMA-NO), S-nitrosoglutathione (GS-NO), and S-nitroso-N-acetyl-D,L-penicillamine (SNAP) results in a potent and dose-dependent increase in the lipid signaling molecule ceramide. Time courses reveal that significant effects occur after 2-4 h of stimulation with NO donors and reach maximal levels after 24 h of stimulation. No acute (within minutes) ceramide production can be detected. When cells were stimulated with NO donors in the presence of phorbol ester, a direct activator of protein kinase C, both ceramide production and DNA fragmentation are completely abolished. Furthermore, addition of exogenous ceramide partially reversed the inhibitory effect of phorbol ester on apoptosis, thus suggesting a negative regulation of protein kinase C on ceramide formation and apoptosis. In contrast to exogenous NO, tumor necrosis factor (TNF)-alpha stimulates a very rapid and transient increase in ceramide levels within minutes but fails to induce the late-phase ceramide formation. Moreover, TNF fails to induce apoptosis in mesangial cells. Interestingly, NO and TNFalpha cause a chronic activation of acidic and neutral sphingomyelinases, the ceramide-generating enzymes, whereas acidic and neutral ceramidases, the ceramide-metabolizing enzymes, are inhibited by NO, but potently stimulated by TNFalpha. Furthermore, in the presence of an acidic ceramidase inhibitor, N-oleoylethanolamine, TNFalpha leads to a sustained accumulation of ceramide and in parallel induces DNA fragmentation. In summary, our data demonstrate that exogenous NO causes a chronic up-regulation of ceramide levels in mesangial cells by activating sphingomyelinases and concomitantly inhibiting ceramidases, and that particularly the late-phase of ceramide generation may be responsible for the further processing of a proapoptotic signal.  (+info)

TNF-alpha increases ceramide without inducing apoptosis in alveolar type II epithelial cells. (8/2471)

Ceramide is a bioactive lipid mediator that has been observed to induce apoptosis in vitro. The purpose of this study was to determine whether endogenous ceramide, generated in response to in vivo administration of tumor necrosis factor-alpha (TNF-alpha), increases apoptosis in primary rat alveolar type II epithelial cells. Intratracheal instillation of TNF-alpha (5 microgram) produced a decrease in sphingomyelin and activation of a neutral sphingomyelinase. These changes were associated with a significant increase in lung ceramide content. TNF-alpha concomitantly activated the p42/44 extracellular signal-related kinases and induced nuclear factor-kappaB activation in the lung. Hypodiploid nuclei studies revealed that intratracheal TNF-alpha did not increase type II cell apoptosis compared with that in control cells after isolation. A novel observation from separate in vitro studies demonstrated that type II cells undergo a gradual increase in apoptosis after time in culture, a process that was accelerated by exposure of cells to ultraviolet light. However, culture of cells with a cell-permeable ceramide, TNF-alpha, or a related ligand, anti-CD95, did not increase apoptosis above the control level. The results suggest that ceramide resulting from TNF-alpha activation of sphingomyelin hydrolysis might activate the mitogen-activated protein kinase and nuclear factor-kappaB pathways without increasing programmed cell death in type II cells.  (+info)

TY - JOUR. T1 - A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. AU - Nickels, Joseph T.. AU - Broach, James R.. PY - 1996/2/15. Y1 - 1996/2/15. N2 - Certain mammalian growth modulators, such as tumor necrosis factor α, interleukin-1β, and γ-interferon, induce an antiproliferative response-terminal differentiation, apoptosisis, or cell cycle arrest-through a novel signal transduction pathway mediated by the lipid ceramide as a second messenger. Both a ceramide-activated protein phosphatase and a ceramide-activated protein kinase have been implicated in transmitting the signals elicited by ceramide. We have determined that ceramide addition to the yeast Saccharomyces causes a similar antiproliferative response, resulting in arrest of cells in the G1 phase of the cell cycle. We have also determined that yeast cells contain a ceramide-activated protein phosphatase composed of regulatory subunits encoded by TPD3 and CDC55 and a catalytic ...
TY - JOUR. T1 - STAT3 mediates C6-ceramide-induced cell death in chronic lymphocytic leukemia. AU - Doshi, Ushma A.. AU - Shaw, Jeremy. AU - Fox, Todd E.. AU - Claxton, David F.. AU - Loughran, Thomas P.. AU - Kester, Mark. N1 - Funding Information: This work was supported by the P01 grant from the National Institutes of Health (P01 CA171983-01 A1) to MK. We thank Nate Sheaffer, Joseph Bednarczyk and Jade Vogel of the Flow Cytometry Core Facility at Penn State College of Medicine for their technical assistance. We also thank Dr Hong-Gang Wang at Penn State College of Medicine for his technical assistance and his lab members for help with reagents. We apologize to those colleagues whose work could not be cited due to space constraints.. PY - 2017. Y1 - 2017. N2 - The pathogenesis of chronic lymphocytic leukemia (CLL) is poorly understood and it remains incurable with current therapies. We have previously shown that nanoliposomal C6-ceramide (CNL) is an effective therapy in an in vivo murine model ...
Our results demonstrated that structural variation among ceramides leads to differences in their behavior in the membranes. The length of the long-chain base of ceramides had a larger effect on ceramide-colipid interactions compared to the effect of N-linked acyl chain length. In addition, the presence of cis-double bonds and their comparative position also caused an important effect on ceramide properties. When we studied how the lateral segregation of ceramides are influenced by the degree of unsaturation of different glycerophospholipids, we observed that these lateral segregations were affected by the overall degree of unsaturation of the sn-2 acyl chain of the PCs. We have also investigated the effect of hydrogen bonding between sphingomyelin (SM) and ceramides or cholesterol interactions. Our findings demonstrated that these interactions are markedly affected by hydrogen bonding properties. Overall, the results of this thesis showed how the gelphase forming properties of ceramides are ...
Spots are localized with the help of commercial standards after primuline spray and eluted by washing the scraped silica gel two times with chloroform/methanol (2/1).. Ceramides either found in free form in cellular extracts or sampled after preparation from more complex lipids are easily studied after derivatization to enable their further quantification or analysis.. Immediately after lipid extraction from tissues or cells, ceramides are directly derivatized with naproxen as for diacylglycerols to give fluorescent (and UV absorbing) compounds. After TLC, the spot corresponding to ceramides is eluted and the extract is thus available for quantification or molecular species analysis.. 2 - Preparation of ceramides from sphingomyelin. A- Ceramides are prepared from sphingomyelin by hydrolysis using phospholipase C from C. welchii as for the production of DAG from other phospholipids and derivatized with naproxen as previously described.. B- It must be recalled that ceramide-containing ...
Apoptosis is a process by which unwanted cells are eliminated in a controlled manner. Early in apoptosis, ceramide levels rise and the mitochondrial outer membrane becomes permeable to proteins. The permeability of the outer membrane is attributed to the self-assembly of ceramide in form of channels. In the only direct structural study, to date, ceramide channels were visualized in liposomes using transmission electron microscopy. Those channels were of various sizes, averaging 10 nm in diameter. In concert, using electrophysiological techniques, the estimated diameter of ceramide channels was also around 10 nm. These channels are large enough to release all the pro-apoptotic intermembrane space proteins to initiate apoptosis. Dihydroceramide desaturase converts the inactive precursor, dihydroceramide to ceramide. Both long and short chain dihydroceramides inhibit ceramide channel formation in mitochondria. The inhibition is strong as one tenth as much dihydroceramide inhibited the outer ...
Macroautophagy is a vacuolar lysosomal catabolic pathway that is stimulated during periods of nutrient starvation to preserve cell integrity. Ceramide is a bioactive sphingolipid associated with a large range of cell processes. Here we show that short-chain ceramides (C(2)-ceramide and C(6)-ceramide) and stimulation of the de novo ceramide synthesis by tamoxifen induce the dissociation of the complex formed between the autophagy protein Beclin 1 and the anti-apoptotic protein Bcl-2. This dissociation is required for macroautophagy to be induced either in response to ceramide or to starvation. Three potential phosphorylation sites, Thr(69), Ser(70), and Ser(87), located in the non-structural N-terminal loop of Bcl-2, play major roles in the dissociation of Bcl-2 from Beclin 1. We further show that activation of c-Jun N-terminal protein kinase 1 by ceramide is required both to phosphorylate Bcl-2 and to stimulate macroautophagy. These findings reveal a new aspect of sphingolipid signaling in up-regulating
Introduction. In this study, we evaluated the anticancer activity of DM102 [(2R,3Z)-N-(1-hydroxyoctadec-3-en-2-yl)pivalamide], a novel inhibitor of the enzyme acid ceramidase (AC), a key regulator of ceramide metabolism, in combination with C6-ceramide (C6-cer) in human breast cancer cells. Ceramide, a bioactive sphingolipid and potent enhancer of apoptosis, is known to play a role in chemo- and radiation-induced cell death. AC tempers ceramide-induced apoptosis by hydrolysis, producing sphingosine and free fatty acid, thus reducing intracellular ceramide levels and providing substrate for sphingosine kinase, which converts sphingosine to sphingosine 1-phosphate. AC has emerged as a putative anticancer target following reports of upregulation in prostate cancer and in some breast tumors. In the present work, we determined whether the introduction of an AC inhibitor would enhance the apoptosis-inducing effects of C6-cer in breast cancer cells.. Methods. Cultured breast cancer cells were ...
TY - JOUR. T1 - Ceramide generation by the reaper protein is not blocked by the caspase inhibitor, p35. AU - Bose, Ron. AU - Chen, Po. AU - Loconti, Andrea. AU - Grüllich, Carsten. AU - Abrams, John M.. AU - Kolesnick, Richard N.. PY - 1998/10/30. Y1 - 1998/10/30. N2 - The Reaper (Rpr) gene encodes a 65-amino acid protein that induces apoptosis in Drosophila by an unknown mechanism. A previous study reported that Rpr expression induced generation of the lipid second messenger ceramide and through use of the peptide caspase inhibitor N-benzyloxycarbonyl-VAD- fluoromethylketone (zVAD.fmk) ordered ceramide generation downstream of caspases in SL2 cells (Pronk, G. J., Ramer, K., Amiri, P., and Williams, L. T. (1996) Science 271, 808-810). The present study re-evaluates these events in SL2 cells transfected with cDNA for Rpr, with or without the baculovirus caspase inhibitor p35, under the control of the metallothionein promoter. Following copper addition, Rpr protein was detected at 1.5 h and ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
Ceramide is a building block for complex sphingolipids in the plasma membrane, but it also plays a significant role in secondary signalling pathways regulating cell proliferation and apoptosis in response to stress. Ceramide activated protein phosphatase activity has been previously observed in association with the Sit4 protein phosphatase. Here we find that sit4Δ mutants have decreased ceramide levels and display resistance to exogenous ceramides and phytosphingosine. Mutants lacking SIT4 or KTI12 display a shift towards nonhydroxylated forms of long chain bases and sphingolipids, suggesting regulation of hydroxylase (SUR2) or ceramide synthase by Sit4p.We have identified novel subunits of the Sit4 complex and have also shown that known Sit4 regulatory subunits-SAP proteins-are not involved in the ceramide response. This is the first observation of separation of function between Sit4 and SAP proteins. We also find that the Sit4p target Elongator is not involved in the ceramide response but ...
Lipid-induced insulin resistance is associated with intracellular accumulation of inhibitory intermediates depending on the prevalent fatty acid (FA) species. In cultured myotubes, ceramide and phosphatidic acid (PA) mediate the effects of the saturated FA palmitate and the unsaturated FA linoleate, respectively. We hypothesized that myriocin (MYR), an inhibitor of de novo ceramide synthesis, would protect against glucose intolerance in saturated fat-fed mice, while lisofylline (LSF), a functional inhibitor of PA synthesis, would protect unsaturated fat-fed mice. Mice were fed diets enriched in saturated fat, n-6 polyunsaturated fat, or chow for 6 wk. Saline, LSF (25 mg/kg . d), or MYR (0.3 mg/kg . d) were administered by mini-pumps in the final 4 wk. Glucose homeostasis was examined by glucose tolerance test. Muscle ceramide and PA were analyzed by mass spectrometry. Expression of LASS isoforms (ceramide synthases) was evaluated by immunoblotting. Both saturated and polyunsaturated fat diets increased
It is known that ceramides can influence the lateral organization in biological membranes. In particular ceramides have been shown to alter the composition of cholesterol and sphingolipid enriched nanoscopic domains, by displacing cholesterol, and forming gel phase domains with sphingomyelin. Here we have investigated how the bilayer content of ceramides and their chain length influence sterol partitioning into the membranes. The effect of ceramides with saturated chains ranging from 4 to 24 carbons in length was investigated. In addition, unsaturated 18:1- and 24:1-ceramides were also examined. The sterol partitioning into bilayer membranes was studied by measuring the distribution of cholestatrienol, a fluorescent cholesterol analogue, between methyl-beta-cyclodextrin and large unilamellar vesicle with defined lipid composition. Up to 15 mol% ceramide was added to bilayers composed of DOPC:PSM:cholesterol (3:1:1), and the effect on sterol partitioning was measured. Both at 23 and 37 degrees C ...
TY - JOUR. T1 - Regulation of insulin action by ceramide. T2 - Dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B. AU - Stratford, Suzanne. AU - Hoehn, Kyle L.. AU - Liu, Feng. AU - Summers, Scott A.. PY - 2004/8/27. Y1 - 2004/8/27. N2 - The sphingolipid ceramide negatively regulates insulin action by inhibiting Akt/protein kinase B (PKB), a serine/threonine kinase that is a central regulator of glucose uptake and anabolic metabolism. Despite considerable attention, the molecular mechanism accounting for this action of ceramide has remained both elusive and controversial. Herein we utilized deletion constructs encoding two different functional domains of Akt/PKB to identify which region of the enzyme conferred responsiveness to ceramide. Surprisingly the findings obtained with these separate domains reveal that ceramide blocks insulin stimulation of Akt/PKB by two independent mechanisms. First, using the isolated pleckstrin homology domain, we found that ...
This week we are featuring SkinMedicas TNS Ceramide Treatment Cream. This product is ideal for dry or post-procedure skin. TNS Ceramide Treatment Cream contains SkinMedicas patented TNS growth factors. These growth factors are proteins that are naturally inherent in our bodies. They play a role in both the activity of our cells and the maintenance of healthy skin. SkinMedicas patented Ceramide technology hydrates the skin and strengthens the skins natural barrier, protecting it from external elements and environmental stressors ...
Gómez-Muñoz, A., Kong, J.Y., Salh, B., Steinbrecher, U.P. (2004). Ceramide-1-phosphate blocks apoptosis through inhibition of acid sphingomyelinase in macrophages. J Lipid Res 45:99-105. [PubMed]Hinkovska Galcheva, V.T., Boxer, L.A.,Mansfield, P.J., Harsh, D.,Blackwood, A., Shayman, J.A.. (1998). The formation of ceramide-1-phosphate during neutrophil phagocytosis and its role in liposome fusion. J Biol Chem 273:50,33203. [PubMed]Gómez-Muñoz, A.,Duffy, P.A., Martin, A., OBrien, L., Byun, H.S., Bittman, R., Brindley, D.N. (1995). Short-chain ceramide-1-phosphates are novel stimulators of DNA synthesis and cell division: antagonism by cell-permeable ceramides. Mol Pharmacol 47:833-9. [PubMed ...
Gómez-Muñoz, A., Kong, J.Y., Salh, B., Steinbrecher, U.P. (2004). Ceramide-1-phosphate blocks apoptosis through inhibition of acid sphingomyelinase in macrophages. J Lipid Res 45:99-105. [PubMed]Hinkovska Galcheva, V.T., Boxer, L.A.,Mansfield, P.J., Harsh, D.,Blackwood, A., Shayman, J.A.. (1998). The formation of ceramide-1-phosphate during neutrophil phagocytosis and its role in liposome fusion. J Biol Chem 273:50,33203. [PubMed]Gómez-Muñoz, A.,Duffy, P.A., Martin, A., OBrien, L., Byun, H.S., Bittman, R., Brindley, D.N. (1995). Short-chain ceramide-1-phosphates are novel stimulators of DNA synthesis and cell division: antagonism by cell-permeable ceramides. Mol Pharmacol 47:833-9. [PubMed ...
Rice Phytoceramide is a plant-sourced phytoceramide that helps provide a plumper, smoother appearance to the skin . Ceramides make up 50% of the lipids in the skins epidermis layer. Increased water loss and skin barrier dysfunction has been linked to decreases in the amount of ceramides in the epidermis. It is known that drier skin is more susceptible to develop lines and wrinkles. The ceramides are absorbed through the digestive process and are carried by blood vessels to the outer layer of the epidermis (stratum corneum) where reinforces the skins ability to retain water, thereby providing the skin with a fuller, smoother appearance ...
As a leader in ceramide technology, Elizabeth Arden has been researching and learning more about ceramides and their role in the skin for 27 years. Research shows that while ceramides are critical to the stratum corneum, other lipids, such as cholest
Skeletal muscles exposed to excess lipid demonstrate decreased sensitivity to insulin. For example, incubating isolated muscle strips or cultured muscle cells with FFAs (22,68-71), infusing lipid emulsions into rodents or humans (72-75), or expressing lipoprotein lipase in skeletal muscle of transgenic mice (76,77) promotes intramyocellular lipid accumulation and compromises insulin-stimulated glucose uptake. To evaluate the role of ceramides in the insulin resistance associated with lipid oversupply, scientists have investigated the lipids role in FFA-induced insulin resistance using cultured myotubes. Schmitz-Pfeiffer et al. (22) first observed that treating C2C12 myotubes with concentrations of saturated FFAs within the physiological serum range increased the intracellular pool of ceramide, while simultaneously inhibiting activation of Akt/PKB, but not PI3K. In this cell type, short-chain ceramide analogs recapitulated this pattern of effects on insulin signal transduction. The authors thus ...
Although previous reports have demonstrated that dietary glucosylceramide can decrease TEWL from human skin [24], the mechanism was unclear. Ueda et al. reported that orally administrated ceramide was distributed to the dermis after intestinal absorption, followed by transfer from the dermis to the epidermis [25]. Ishikawa et al. also showed that dietary glucosylceramide was degraded into sphingoids, which were absorbed through the intestinal epithelial cells of rats and were subsequently also found in the lymph fluid in these rats [11]. Another study showed that 4-hydroxysphinganine could activate the PPARs [26] that are the key regulators of keratinocyte differentiation [27].. Keratinocyte differentiation is closely related to internal ceramide synthesis [28]. It is unlikely that orally ingested glucosylceramide would specifically localize to the skin because the amount of ingested glucosylceramide is simply too little to enhance ceramide levels in the skin. We hypothesized that sphingoids are ...
Although ceramides (Cers) are key constituents of the epidermal permeability barrier, they also function as apoptogenic signals for UVB irradiation-induced apoptosis in epidermal keratinocytes. As epidermis is continuously exposed to UV irradiation,
In this study, we found increased sphingomyelinase activity and accumulation of long‐chain ceramides in small arteries from old sheep compared to young. Additionally, we observed an increase in total collagen content in arteries from old animals. Ceramide promotes collagen deposition in dermal fibroblasts (Sato et al. 2003) implicating this lipid in the extracellular matrix remodeling of small arteries in age.. Investigation of the morphology and mechanical properties of small arteries showed that under passive conditions the mesenteric small arteries from old sheep had increased lumen diameter and media thickness without a change in media to lumen ratio, indicative of outward hypertrophic remodeling (Mulvany 1999). In addition, there was deposition of collagen in a thickened intima layer that contained both endothelial and smooth muscle cells in arteries from old animals. However, despite increased collagen content and decreased elastin to collagen ratio there was no change in stiffness in ...
Sphingomyelin (SM) and its metabolic products are now known to have second messenger functions in a variety of cellular signaling pathways. Particularly, the sphingolipid metabolites, ceramide (Cer) and sphingosine-1-phosphate (S1P), have emerged as a new class of potent bioactive molecules. Ceramide can be generated de novo or by hydrolysis of membrane sphingomyelin by sphingomyelinase (SMase). Ceramide is subsequently metabolized by ceramidase to generate sphingosine (Sph) which in turn produces S1P through phosphorylation by sphingosine kinases 1 and 2 (SphK1, 2). Both ceramide and S1P regulate cellular responses to stress, with generally opposing effects. S1P functions as a growth and survival factor, acting as a ligand for a family of G protein-coupled receptors, whereas ceramide activates intrinsic and extrinsic apoptotic pathways through receptor-independent mechanisms ...
Dysregulated lipid metabolism and inflammation are linked to the development of insulin resistance in obesity, and the intracellular accumulation of the sphingolipid ceramide has been implicated in these processes. Here, we explored the role of circulating ceramide on the pathogenesis of insulin resistance. Ceramide transported in LDL is elevated in the plasma of obese patients with type 2 diabetes and correlated with insulin resistance but not with the degree of obesity. Treating cultured myotubes with LDL containing ceramide promoted ceramide accrual in cells and was accompanied by reduced insulin-stimulated glucose uptake, Akt phosphorylation, and GLUT4 translocation compared with LDL deficient in ceramide. LDL-ceramide induced a proinflammatory response in cultured macrophages via toll-like receptor-dependent and -independent mechanisms. Finally, infusing LDL-ceramide into lean mice reduced insulin-stimulated glucose uptake, and this was due to impaired insulin action specifically in ...
When it comes to your skin, ceramides help create a barrier to prevent permeability. This locks moisture into your skin, preventing it from becoming dry and irritated. Read on to learn how else your skin might benefit, who needs ceramides most, how to choose the right products, and more.
AA GOLDEN CERAMIDES is a comprehensive treatment inspired by the effects of cosmetic sonophoresis. The richness of active components, such as ceramides, 24 carat cosmetic gold, oligopeptides, and hyaluronic acid neomatrix is a truly luxurious mixture that makes your skin regain exceptional smoothness and firmness. Increased effectiveness of treatment is ensured thanks to an intelligent fibre damage tracking system (INT GC3), which releases sequences of active components depending on individual needs of the skin.
Invigorate your skin with Pure Creations Elasticity Boost Eye Cream with Ceramides. This wonderful blend of ceramides, hyaluronic acid and shea butter promotes elasticity while treating dehydrated and inflamed skin.
This ceramide-rich cream contains 2% ceramides and stabilised vitamin C, retinol and hyaluronic acid to provide intense hydration that absorbs easily and improves skin smoothness, reduces wrinkles and boosts cutaneous elasticity.
Vesicle-associated membrane proteinCassociated protein (VAP) is definitely an endoplasmic reticulum (ER)-resident integral membrane protein that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to the Golgi complex by interacting with ceramide transfer protein and oxysterol-binding protein (OSBP), respectively. As demonstrated in Number 6A, endogenous VAP-A and VAP-B were coprecipitated with FLAG-Sac1 (lane 7). FLAG-Sac1 showed a higher affinity for VAP-A than VAP-B, which is definitely consistent with more proclaimed colocalization of Sac1 with VAP-A than VAP-B at the juxtanuclear storage compartments (Number 4A), although 25-Oh yea treatment did not significantly impact their relationships (Number 6A, lane 8). Additional Emergency room integral membrane proteins, calnexin and valosin-containing protein-interacting membrane protein (VIMP), were not coprecipitated with FLAG-Sac1 (Number 6A, lanes 7 and 8), suggesting that the interaction of VAPs with FLAG-Sac1 is ...
Evonik has developed a biotechnological green production process to produce ceramides for cosmetics that is based on the fermentation of the yeast Wickerhamomyces ciferrii (formerly known as Pichia ciferrii).
This week we are featuring SkinMedicas TNS Ceramide Treatment Cream. This product is ideal for dry or post-procedure skin. TNS Ceramide Treatment Cream contains SkinMedicas patented TNS growth factors. These growth factors are proteins that are naturally inherent in our bodies. They play a role in both the activity of our cells and the maintenance of healthy skin. SkinMedicas patented Ceramide technology hydrates the skin and strengthens the skins natural barrier, protecting it from external elements and environmental stressors ...
Bringing the best of both worlds together with Ceramides and Liposomes to help hydrate and nourish your skin. Skin ceramides make up 35-40% of the lipids in the extracellular cement that binds together protective cells in the outermost skin layer, the stratum corneum. Loss of skin ceramides renders aging skin permeable to moisture, leaving skin dry, rough, and itchy. Ceramide loss is also a major contributor to wrinkles.
The positive results from the Single Dose Tolerance study allows us to proceed to the next stage of the Phase I clinical development with a Multiple Dose Safety and Tolerance study. Given the current lack of treatment options for NAFLD and NASH, CER-209 has the potential to play an important role in treating hepatic steatosis and atherosclerosis. CER-209s major asset in NAFLD and NASH treatment lies in its ability to promote HDL recognition and lipid elimination by the liver, through the activation of natural metabolic pathways mediated by the P2Y13 receptor. In addition, the studys confirmation that the pharmacokinetics of CER-209 permit once daily oral dosing is excellent news for patient treatment, said Dr. Jean-Louis Dasseux, CEO of Cerenis ...
with Ceramides 1,3 and 6 II. Cera Repair Cream contains Advanced Biomimicry Technology. It is very moisturizing and is clinically proven to reduce skin roughness and improve skin texture.. ...
Shop our community curated line of eye serums with ceramides, including Medik8 Pretox Eyelift at Truth in Aging. Order today to receive free US shipping!
Dr. Guttermans investigative interests focus on regulation of human vascular reactivity both at the fundamental and translational research levels. His work has defined a novel mechanism whereby a switch occurs in the mediator of flow-induced dilation from nitric oxide to shear-released mitochondria-derived hydrogen peroxide in the microcirculation of patients with coronary artery disease. The cellular mechanism of this switch is under active investigation with emerging roles for extranuclear telomerase, short chain ceramides, and autophagy as key modulators of mitochondrial ROS ...
Ethical Permission. This study was approved by the Ethics committee at Karolinska Institutet (Etikkommitté syd).. Reagents. SR141716A and SR144528 were kind gifts from Sanofi-Aventis (Montpellier, France). R(+)-Methanandamide was purchased from Tocris Cookson Inc. (Bristol, UK). Win 55,212-2 mesylate, Fumonisin B1, l-CS, and z-VAD-FMK were purchased from Sigma Aldrich (St Louis, MO). SB202190 and SB203580 were purchased from Calbiochem Inc. (San Diego, CA), and 3,3′-dihexyloxacarbocyanine iodide was purchased from Invitrogen (Carlsbad, CA). R(+)-MA was dissolved to 10 mM in EtOH and the other reagents were dissolved to 10 mM in dimethyl sulfoxide. Further dilutions in culture medium were made on the day of the experiment.. Tumor Tissue and Controls. Tumor tissue from MCL was obtained from cryopreserved patient samples with more than 90% purity of tumor B cells. Three samples (L718, L1547, and L1676) were collected from tonsil, lymph node, and peripheral blood, respectively, of three MCL ...
Supplementary MaterialsVideo S1. Apical ECM/BM effects modeled as viscous resistances and exterior viscous level of resistance coefficient put on both areas at 8,000?Pa s m?1. Range bar is normally 20?m. Simulation period depicted over the structures. mmc3.mp4 (2.0M) GUID:?63F04E92-06A0-488F-8AD5-FA7674E03304 Video S3. Appropriate Flip Morphology Emerges with Planar Differential Development Prices and an 1187594-09-7 Explicit Description …Read More. ...
Supplementary MaterialsSupp FigS2. copy number of the mtDNA genes and by qPCR in RVH individuals before and 24hrs after PTRA, performed during IV infusion of vehicle (n=8) or the mitoprotective drug elamipretide (ELAM, 0.05mg/kg/hr, n=6). Five healthy volunteers (HV) served as settings. Urinary mtDNA levels YM-90709 were also assessed in RVH and normal pigs (n=7 …Read More. ...
My current research activity (x35) is as follows: Invited Talks and Posters (22) American Diabetes Association (ADA) Annual Meeting, McCormick Place Convention Center (West Building), Chicago, IL; Potential for Alterations in Islet Beta-Cell Sphingolipid/Ceramide Axis Contributing to ER Stress Models of Diabetes, 21-25th June 2013 Southeastern Regional Lipid Conference (SERLC), Cashiers, North Carolina, 7-9th November…
The central executioner of apoptosis: multiple connections between protease activation and mitochondria in Fas/APO-1/CD95- and ceramide-induced apoptosis.: Acco
First of all I would like to start with the appearance of the product. The jar looks awesome! White colored tub packaging looks very minimalist and futuristic. With the product you also get a little plastic spatula which helps you keep your product uncontaminated for a little longer.. Texture of a moisturizer is white and gel-ish. It has a typical non-scented chemical smell which I like. I apply the gel after I washed, toned and nourished my skin with serums and essences. When I patted it into my skin it absorbed really quickly (5-8 min).. The other day I put it on way more and it needed longer time to absorb, and that day I skipped serums and essences, because it claims that it can provide five basic skincare elements: Lotion, Beauty Serum, Emulsion, Cream, and Mask.. ...
Apply Ceramide Capsules Daily Youth Restoring Serum to your face, neck and decolletage to retexturize and revitalize your skin with youth-restoring ceramides. This is lightweight, silky-smooth yet non-greasy formula.
RatzillaCosme is a complete and comprehensive guide since 2009 to Japanese cosmetics and beauty products. Everything on this site (including ingredients) is outlined by me based on official Japanese press releases ...
Our online collection of ceramide products for facial skincare help retain moisture to reduce fine lines, improves hydration and fights the signs of aging.
Ceramides are a family of waxy lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high concentrations within the cell membrane of cells, since they are component lipids that make up sphingomyelin, one of the major lipids in the lipid bilayer. Contrary to previous assumptions that ceramides and other sphingolipids found in cell membrane were purely supporting structural elements, ceramide can participate in a variety of cellular signaling: examples include regulating differentiation, proliferation, and programmed cell death (PCD) of cells. The word ceramide comes from the Latin cera (wax) and amide. Ceramide is a component of vernix caseosa, the waxy or cheese-like white substance found coating the skin of newborn human infants. There are three major pathways of ceramide generation. The sphingomyelinase pathway uses an enzyme to break down sphingomyelin in the cell membrane and release ceramide. The de novo pathway creates ceramide from less complex ...
Background: Among the 5 (L1-L5) chromatographically resolved subfractions of human LDL, L5 is the most electronegative and atherogenic. L5s atherogenicity may be attributed in part to its high ceramide content. Because ceramides can be converted from sphingomyelins by sphingomyelinase (SMase), we examined whether L5 has SMase-like properties.. Methods and Results: The total lipid extract of L5 was as capable as L5 in inducing arterial endothelial cell (EC) apoptosis. LC/MSE analysis confirmed that L5 had a significantly greater ceramide content in the lipid extract than L1 (n=4; P,0.01). On Amplex Red SMase assays, L5 but not L1 exhibited a strong SMase-like activity (n=12). ECs treated with L5 increased intracellular ceramide concentrations; the associated cytotoxicity was significantly attenuated by SMase inhibitor desipramine. To identify the putative region of LDL with SMase activities, we compared human apolipoprotein B100 (apoB100) with Bacillus cereus, Leptospira interrogans, and ...
Ceramide synthase 5 (CerS5) is the enzyme encoded in humans by the CERS5 gene. CerS5 robustly synthesizes C16-ceramide, which is often considered to be an important pro-apoptotic ceramide. De novo ceramide synthesis is an essential trigger for Bax activation in hypoxia/reoxygenation. Following hypoxia/reoxygenation, CerS5 expression is elevated. Upon knocking down acid sphingomyelinase and CerS5 in NTERA-2 cells, Bax localization to mitochondria was reduced, indicating the importance of CerS5 activity in the apoptosis pathway. CerS5 (TRH4) mRNA is found in all tissues and is strongly expressed in muscle and brain. CerS5 is the major ceramide synthase detected in lung epithelia. Knock-down research in respiratory epithelium using CerS5 siRNA or fumonisin B1 reduced total CerS activity by 45% or 78%, respectively, indicating that CerS5 indeed contributes significantly to ceramide synthesis in lung. In the brain, CerS5 mRNA is detected in most cells within the gray and white matter tissues. CerS5 ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
Ceramides are the main polar lipids of the SC intercellular lipids; they play an important role in skin barrier function and account for half of the SC lipids by weight. Nine subclasses of ceramides have been described in human SC; these differ from each other by the sphingoid base groups (sphingosine, phytoshpingosine, 6-hydroxysphingosine) and hydrocarbon chain length. Ceramide content and changes of composition are involved in agingand skin diseases such as atopic dermatitis (AD),5-7 psoriasis, contact dermatitis (CD) and irritant dermatitis.
AIMS/HYPOTHESIS: An accumulation of ceramides has been implicated in the generation of insulin resistance in skeletal muscle upon an oversupply of fatty acid. Different ceramide species are generated through the actions of ceramide synthases (CerSs), which incorporate specific acyl side chains. We tested whether particular CerS isoforms promoted insulin resistance through the generation of more inhibitory ceramide species, thus representing potential targets for intervention. METHODS: CerS isoforms CerS1, CerS2, CerS4, CerS5 and CerS6 were overexpressed in L6 myotubes using adenovirus, and cells were treated with palmitate and stimulated with insulin. Alternatively, CerS isoforms were knocked down using siRNAs. Sphingolipids were examined by mass spectrometry and tracer incorporation. Phosphorylation of IRS1 and Akt was measured by immunoblotting, while glucose disposal was assessed by measuring GLUT4 translocation and the incorporation of [(14)C]glucose into glycogen. RESULTS: Palmitate treatment
Sphingolipids such as ceramide and S1P have been recognized as bioactive lipids in cell death and proliferation/survival. In this review we overviewed role for sphingolipids in the induction of cell death in several types of hematological malignancies such as leukemia, malignant lymphoma and multiple myeloma. The main backbone molecule of sphingolipid network is ceramide. Ceramide is synthesized from serine and fatty acids with different kinds of carbon chains by de novo ceramide pathway in endoplasmic reticulum, and transferred to the Golgi apparatus by CERT for synthesis of SM by SMS while ceramide transfer protein for glucosylceramide to Golgi apparatus remains unknown. Sphingosine generated by CDase is phosphorylated and turns to S1P by SK1 or SK2. The complex sphingolipids such as glucosylceramide and GM3 are transferred to the micro-organelles to exert their functions and again returned to ceramide by salvage pathway. Likely, the catabolized sphingolipids such as sphingosine and ...
Sphingolipids play critical roles in signal transduction, intercellular membrane trafficking and cell growth. As bioactive sphingolipids, ceramide and sphingosine have been implicated in activating anti-proliferative and apoptotic responses in various cancer cells. Conversely, metabolic conversion of ceramide into sphingosine 1-phosphate, ceramide 1-phosphate and glucosylceramide regulates cell proliferation and suppresses ceramide programmed cell death. Many anticancer drugs and stress-induced agonists have been developed to increase endogenous ceramide levels. Sphingosine/ceramide analogues reportedly enhance antitumor activity and have been proposed as a potential new class of chemotherapeutic agents. Among these, sphingosines with aromatic substituents in the side chain often exhibit stronger biological activity compared to natural sphingosines. While a large number of synthetic pathways to sphingosine analogues have been described in the literature, very few pathways provide analogues with ...
Cigarette smoking is a common and lethal worldwide habit, with considerable mortality stemming from its deleterious effects on heart function. While current theories posit altered blood lipids and fibrinogen metabolism as likely mediators, none have explored the role of the sphingolipid ceramide in exacerbating heart function with smoke exposure. Ceramide production is a consequence of cigarette smoke in the lung, and considering ceramides harmful effects on mitochondrial function, we sought to elucidate the role of ceramide in mediating smoke-induced altered heart mitochondrial respiration. Lung cells were exposed to cigarette smoke extract and heart cells were exposed to the lung-cell conditioned medium. Adult male mice were exposed sidestream cigarette smoke for 8 weeks with dietary intervention and ceramide inhibition. Ceramides and heart cell or myocardial mitochondrial respiration were determined. Lung cell cultures revealed a robust response to cigarette smoke extract in both production and
We investigated sphingolipid metabolism in human adipose tissue to identify pathways underlying increased ceramide concentrations in inflamed adipose tissue [3]. Our data suggest that hydrolysis of sphingomyelin to ceramide by sphingomyelinases could explain, at least partly, this increase. Gene expression levels of SMPD3 correlate significantly with concentrations of various ceramides and sphingomyelins in subcutaneous adipose tissue, and are higher in the relatively more inflamed intra-abdominal compared to the subcutaneous depot in both obese and non-obese subjects. Sphingomyelinases are expressed by both adipocytes and macrophages in adipose tissue, but their expression is strongest in and around blood vessels. Our findings implicate a role for sphingomyelinase-mediated generation of ceramide in adipose tissue inflammation.. When comparing inflamed ceramide-rich and relatively less inflamed ceramide-poor subcutaneous adipose tissue of obese women, we report here for the first time that there ...
using yeast to study the role of ceramide pathway in the regulation of mammalian protein kinase c isoforms dissertação de mestrado em genetica molecul
Cystic fibrosis, a genetic disease associated with frequent lung infections and a shortened life span, is caused by a defect in the CFTR gene, which encodes a membrane transporter. Although it is not clear exactly how defective CFTR links to the symptoms, the mutant protein is known to increase the pH in intracellular organelles. On the basis of results obtained from patients cells and from mice carrying mutated Cftr (which produces a cystic fibrosis-like disease), Teichgräber et al. suggest that this rise in pH increases susceptibility to lung infection by altering levels of ceramide, a membrane constituent that can also trigger cell death. The higher pH inhibits the enzyme that breaks down ceramide, and the resulting excess of ceramide increases vulnerability to lung infection. Blocking the biosynthesis of ceramide via acid sphingomyelinase normalizes ceramide levels and, most tellingly, renders the Cftr-deficient mice resistant to lung infections. This block can be achieved with ...
Increased availability of free fatty acids impairs glucose disposal in young healthy humans. Patients with type 2 diabetes have reduced whole body glucose disposal, increased ectopic lipid deposition in skeletal muscle and the liver and impaired mitochondrial function. Recent studies suggest that lipid metabolites such as diacylglycerol (DAG), ceramides and long-chain acyl-coA represent the active mediators inducing insulin resistance. Possible targets are DAG-sensitive Proteinkinase C (PKC θ, PKC ε) which inhibit the insulin signaling cascade and ceramides which interfere with the insulin signaling cascade at Proteinkinase B/AKT. Prior studies raised controvesial evidence, thus, it is yet unclear, whether DAG or ceramides are the primary agents inducig lipid-induced insulin resistance. Therefore, the current study aims to explore the time course of the appearance of intramyocellular lipid compunds during lipid infusion in parallel assessing markers of impaired insulin action ...
Current investigations are giving new insight about the roles of sphingolipids in cellular regulation of growth suppression, apoptosis and signal transduction. Among the sphingolipids, ceramide has emerged as an important second signal mediator, having multiple cellular and biochemical targets. In particular, ceramide act as a potent cell death inducer and a specific cell growth suppressor. Recently, it has been thought that generation of ceramide is a universal feature of apoptosis [6, 44]. Presently, a few drugs are available in combinatorial chemotherapy, which could generate ceramide. Therefore, to find pharmacological modulators that induce ceramide production in malignant cells by mounting a physiological response and induce senescence is on prime importance.. In the present study, we demonstrated rapid accumulation of endogenous ceramide in WithaD treated MOLT-4 cells. In fact, 61.55% cells were ceramide positive just in 30 mins. WithaD showed almost four fold increase in ceramide level ...
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Ceramide, a key intermediate in sphingolipid metabolism, is synthesized by acylation of sphinganine followed by dehydrogenation of dihydroceramide to ceramide. Using radioactive sphinganine, we have examined the site and topology of dihydroceramide synthesis in well-characterized subcellular fractions from rat liver. [4,5-3H]Sphinganine was introduced as a complex with BSA and was metabolized to [4,5-3H]dihydroceramide upon incubation of rat liver homogenates or microsomes with fatty acyl CoA. Conditions were established in a detergent-free system in which dihydroceramide synthesis was not limited by either substrate availability or by amounts of microsomal protein or reaction time. The distribution of dihydroceramide synthesis was found to exactly parallel that of an endoplasmic reticulum (ER) marker upon subfractionation of microsomes, and no endogenous activity was detected in either purified Golgi apparatus or plasma membrane fractions. Limited protease digestion demonstrated that ...
Phytoceramides were recently featured on the Dr. Oz television show, and since that time, they have been incredibly popular. Phytoceramides are used to help fight the aging process, reduce wrinkles, and prevent fine lines from occurring. What is interesting about Phytoceramides Walgreens is that they are completely natural ingredients and that they really do work.. Dr. Oz Phytoceramides are considered to be the most effective of these anti-aging pills, as they help to reintroduce plant derived ceramides into the system. Ceramides are the natural protein molecules that help to keep the moisture barrier within the skin intact so that the skins health can be improved.. ...
Numerous prior studies suggest a close relationship between the regulation of ceramide levels through GCS and the induction of apoptosis. First, the GCS inhibitor 1-phenyl-2-decanoylamino-3- morpholino-1-propanol enhanced apoptosis in the presence of C6-ceramide in CHP-100 human neuroepithelioma cells (30) . Second, increased GCS protein levels and activity are evident in DOX-resistant human breast cancer cells (MCF-7-AdrR; ref. 31 ), and inhibition of GCS (using antisense) reversed resistance to DOX in these cells (32) . Third, overexpression of GCS conferred resistance to DOX in MCF-7 or HL-60 cells (19 , 33) . Finally, overexpression of GCS induced resistance to ceramide-induced apoptosis in human hepatoma Huh 7 cells (20) and attenuated ceramide-induced growth inhibition in human keratinocytes (21) . Moreover, our prior studies showed that the relative GCS activity is approximately 10- to 100-fold higher than that of other ceramide-metabolizing enzymes, such as sphingomyelin synthase and ...
TY - CHAP. T1 - Lung cancer and lung injury. T2 - The dual role of ceramide. AU - Goldkorn, Tzipora. AU - Chung, Samuel. AU - Filosto, Simone. PY - 2013. Y1 - 2013. N2 - Sphingolipids play key roles in cancer, yet our current understanding of sphingolipid function in lung cancer is limited to a few key players. The best characterized of these are sphingosine-1-phoshate and ceramide which are described for their opposing roles in cell fate. However, because sphingolipids as a whole are readily interconverted by a complex enzymatic machinery, no single sphingolipid appears to have exactly one role. Instead, the roles of specific sphingolipids appear to be context specific as demonstrated by findings that ceramide-1-phosphate has both proliferative and apoptotic effects depending on its concentration. Therefore, we present herein several years of research on ceramide, a sphingolipid linked to apoptotic signaling, that is emerging in cancer research for its potential roles in proliferation and ...
Explore the benefits of ceramides in skincare. Ceramides maintain skin-hydration & have been suggested to have soothing properties that can alleviate dry skin.
From this experience, I realised the importance of using the right product which is suited for sensitive skins. It was something I had overlooked and thinking back, if only I had known it earlier…. I wouldnt have to go through those episodes. Sensitive skin is actually caused by a lack of ceramide, which is an essential ingredient in the skins as it helps to replenish moisture between the skin cells. Ceramides are naturally found within our skins, however sometimes our body dont produce enough of it, leading us to have ceramide-deficient skin. When this happens, our skin is likely to be aggravated by external irritation, causing problems like dryness, chapping, redness and itch ...
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear ...
Sphingolipids are major constituents of membranes. A number of S. cerevisiae sphingolipid intermediates such as long chains sphingoid bases (LCBs) and ceramides act as signaling molecules regulating cell cycle progression, adaptability to heat stress, and survival in response to starvation. Here we …
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Ceramides play a central role in preserving healthy-looking skin. They are an important lipid component of the skins membrane‚ where they provide protection from the elements and lock in moisture. In fact‚ they make up 35%-40% of the binding matrix that maintains moisture balance and protects the skins surface. The problem is that the bodys production of ceramides declines with age. The ceramides that young skin naturally produces to retain its supple appearance are identical to those present in wheat‚ which is why wheat-derived oils have been used topically for centuries as a natural moisturizer. However‚ you cant get enough ceramides from topically-applied wheat germ oil to have a long-term impact on your skins appearance‚ and they dont appear in sufficient concentration in your diet. Thats where a supplement like Life Extensions Skin Restoring Ceramides with Lipowheat can help. This product brings together skin-nourishing oils in an oral formula with Lipowheat™‚ a ...
Acid sphingomyelinase-induced ceramide release has been shown by many studies to induce apoptosis in response to various stimuli. However, the mechanisms of acid sphingomyelinase/ceramide-mediated dea
Skin Restoring Phytoceramides with Lipowheat. Ceramides play a central role in preserving healthy-looking skin. They are an important lipid component of the skins membrane, where they provide protection from the elements and lock in moisture. In fact, they make up 35%-40% of the binding matrix that maintains moisture balance and protects the skins surface. The problem is that the bodys production of ceramides declines with age.. The ceramides that young skin naturally produces to retain its supple appearance are identical to those present in wheat … which is why wheat-derived oils have been used topically for centuries as a natural moisturizer. But you cant get enough ceramides from topically-applied wheat germ oil to have a long-term impact on your skins appearance. And they dont appear in sufficient concentration in your diet.. So weve brought together these skin-nourishing oils in an oral formula with Lipowheat, a proprietary phytoceramide blend that offers nutritional support for ...
This was a laboratory study, primarily conducted in mice, though there was an aspect of human-cell analysis. The researchers were trying to get a better understanding about the biological processes that take place in the lungs when an organism has cystic fibrosis. Cystic fibrosis is the most common inherited life-threatening disease that occurs among Caucasian people in the UK. In an experiment conducted in several different parts, the researchers used strains of mice with a mutation that gave them cystic fibrosis. They were particularly interested in the role of a particular lipid called ceramide. In the first part of the experiment, the researchers compared the levels of ceramide in the lungs of the mutant mice with the levels in healthy mice. They did further experiments to identify in exactly which cells ceramide was accumulating. The researchers also looked at whether the ceramide concentration had any effect on pH (i.e. acidity) levels in the respiratory cells. The researchers were testing ...
NIACINAMIDE SerumReduces water loss by 24% in 4 weeks, boosts fatty acid and ceramides by 67% and 34% respectively; stimulates new fibroblasts by 20%
NIACINAMIDE Serum (anti-aging serum)Reduces water loss by 24% in 4 weeks, boosts fatty acid and ceramides by 67% and 34% respectively; stimulates new
Collagen30 Tablets with Ceramides, 120 units. Discover the variety of products, detailed information, prices and availability in stores or online.
OGT2378 is a GCS inhibitor (Glucosyl Ceramide Synthase Inhibitor) with potential anticancer activity. In murine models with melanoma, OGT2378 decreased tumor size, and showed no cytotoxic and apoptotic effects on MEB-4 melanomacells. Additionally, it has been reported that OGT2378 is biologically active, and a well tolerated agent in in vivo system.
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
In this book many aspects of ceramide and sphingolipid biology are addressed. The focus of this book is the metabolism of ceramide in normal and diseased states and the biophysical and biochemical mechanisms governing the bioactivity of these molecules.
The primary objective of this study is to obtain data regarding the safety of olipudase alfa in patients with acid sphingomyelinase deficiency (ASMD) who are exposed to long term treatment with olipudase alfa.. The secondary objectives of this study are to obtain data regarding the efficacy of olipudase alfa and to characterize olipudase alfa pharmacodynamics (PD) and pharmacokinetics (PK) following long-term administration. ...
Cloud Ceramide Balm delivers an extra boost of nutrient-rich moisture where skin needs it most Details Organic Rice Micro Ceramides absorb deeply into the skin to replenish and restore the moisture barrier. This plant superpower lipid strengthens skins natural bond while increasing collagen production and leaving skin
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
With new product brands, launches, and categories popping up every day, beauty can be a bit overwhelming. Back to Basics, our new rudimentary beauty series, serves as your crash course on the science behind some of the best formulations in the…
First luxurious anti-wrinkle care from AA brand with a division into skin types. The ageing process of the skin does not depend entirely on age, but also on many other factors: genes, lifestyle, diet, external environmental factors and solar radiation.
Posted on 08/07/2013 7:35:02 PM PDT by CutePuppy. The scientists discovered that the ceramide-1 phosphate transport protein (CPTP) regulates levels of biologically active lipids, which are molecules such as fatty acids that often play a role in cell signaling. They found that CPTPs main function is to transport ceramide-1-phosphate (C1P), a lipid that helps regulate cell growth, survival, migration and inflammation. Specifically, C1P increases the production of pro-inflammatory eicosanoids - powerful signaling molecules that contribute to chronic inflammation in diseases such as cancer, asthma, atherosclerosis and thrombosis - and the discovery of CPTP sheds a light on the cellular mechanisms that contribute to these diseases. We may have identified the newest target for treating cancer. Because of the important role this protein plays in a number of cellular functions, it could also have large implications for a variety of diseases like cancer that are caused by inflammation, The team was ...
We have investigated whether the increases in ceramide levels that occur during apoptosis in SKW 6.4 cells induced by anti-Fas antibody depend on the activation of caspases. Using cells prelabelled to equilibrium with ...
Áhrifaríkt andlitskrem sem veitir húðinni mikilvægar olíur, inniheldur ceramides, kólestról og fitusýrur. Nærir húðina og dregur úr öldrunareinkennum. Húðin fær aukinn ljóma, jafnari og þéttari áferð Hentar fyrir þroskaða, normal og þurra húðgerð Einstök létt áferð sem frásogast hratt inn í húðina Engin paraben eða lit
E & C, lycopene-rich tomato extract and ceramides.Suitable for all skin types, even with extremely dry skin caused by neuro-dermatitis. Skin feels noticeably firmer and smoother. ...
Ceramide synthases include: Ceramide synthase 1 Ceramide synthase 2 Ceramide synthase 3 Ceramide synthase 4 Ceramide synthase 5 ... ceramide. CerS are involved in the de novo synthesis pathway of ceramides. Their role is acylation coupling of sphinganine to a ... Ceramide synthases are integral membrane proteins of the endoplasmic reticulum. This enzyme belongs to the family of ... Stiban J, Tidhar R, Futerman AH (2010) Ceramide synthases: roles in cell physiology and signaling. Adv Exp Med Biol 688: 60-71 ...
Some skin ceramides are proteolipids. The amino group on lysine can also be myristoylation via a poorly-understood mechanism. ...
It is unclear if moisturizers that contain ceramides are more or less effective than others. Products that contain dyes, ... Jungersted JM, Agner T (August 2013). "Eczema and ceramides: an update". Contact Dermatitis. 69 (2): 65-71. doi:10.1111/cod. ...
Recommendations to reduce or limit dietary intake of saturated fats are made by the World Health Organization,[72] American Heart Association,[6] Health Canada,[73] the US Department of Health and Human Services,[74] the UK Food Standards Agency,[75] the Australian Department of Health and Aging,[76] the Singapore Government Health Promotion Board,[77] the Indian Government Citizens Health Portal,[78] the New Zealand Ministry of Health,[79] the Food and Drugs Board Ghana,[80] the Republic of Guyana Ministry of Health,[81] and Hong Kong's Centre for Food Safety.[82] In 2003, the World Health Organization (WHO) and Food and Agriculture Organization (FAO) expert consultation report concluded that "intake of saturated fatty acids is directly related to cardiovascular risk.[83] The traditional target is to restrict the intake of saturated fatty acids to less than 10% of daily energy intake and less than 7% for high-risk groups. If populations are consuming less than 10%, they should not increase that ...
... s are primarily used as surfactants, usually in the form of emulsifiers. Together with diglycerides, monoglycerides are commonly added to commercial food products in small quantities as E471, which helps to prevent mixtures of oils and water from separating. The values given in the nutritional labels for total fat, saturated fat, and trans fat do not include those present in mono- and diglycerides as fats are defined as being triglycerides. They are also often found in bakery products, beverages, ice cream, chewing gum, shortening, whipped toppings, margarine, and confections.[5] In bakery products, monoglycerides are useful in improving loaf volume and texture, and as antistaling agents.[6][7] Monoglycerides are used to enhance the physical stability towards creaming in milk beverages.[8] ...
Ceramide (CAPPs, KSR1, PKCζ, CTSD). *Glucosylceramides. *S1P. *Sphingosine (SDK1, PKH, YPK) ...
It is also known as CD77, Gb3, and ceramide trihexoside.[2] It is one of the few clusters of differentiation that is not a ... "The role of ceramide trihexoside (globotriaosylceramide) in the diagnosis and follow-up of the efficacy of treatment of Fabry ...
Moderate and high quality evidence from a 2020 Cochrane Systematic Review has shown that EPA and DHA, such as that found in Omega‐3 polyunsaturated fatty acid supplements, does not appear to improve mortality or cardiovascular health.[19] There is very weak evidence to suggest that α-linolenic acid may be associated with a small reduction in the risk of a cardiovascular event or the risk of arrhythmia.[19] A 2018 meta-analysis found no support that daily intake of one gram of omega-3 fatty acid in individuals with a history of coronary heart disease prevents fatal coronary heart disease, nonfatal myocardial infarction or any other vascular event.[8] However, omega−3 fatty acid supplementation greater than one gram daily for at least a year may be protective against cardiac death, sudden death, and myocardial infarction in people who have a history of cardiovascular disease.[20] No protective effect against the development of stroke or all-cause mortality was seen in this population.[20] A ...
... and two types of acetooleins have been found to be without toxic effects in long-term feeding tests in rats at levels that were several orders of magnitude greater than those to which consumers are exposed. Three types of acetostearins have been found to be without toxic effects in long-term feeding tests in rats at levels up to 5 g per kg per day. This contrasts with an estimated human consumption of a fraction of a milligram per kg per day. It is recognized that at an even higher feeding level (10 g per kg per day) male rats developed testicular atrophy and female rats, uterine discoloration. However, such a level which would amount to 50 g or more for an infant and 600 g for an adult per day, is vastly higher than would be possible in the consumption of foods to which acetostearins are added for functional purposes. ...
Being inexpensive and chemically benign, stearic acid finds many niche applications. As an example, it can be used to make castings from a plaster piece mold or waste mold, and to make a mold from a shellacked clay original. In this use, powdered stearic acid is mixed in water and the suspension is brushed onto the surface to be parted after casting. This reacts with the calcium in the plaster to form a thin layer of calcium stearate, which functions as a release agent. When reacted with zinc it forms zinc stearate, which is used as a lubricant for playing cards (fanning powder) to ensure a smooth motion when fanning. Stearic acid is a common lubricant during injection molding and pressing of ceramic powders.[12] It is also used as a mold release for foam latex that is baked in stone molds. Stearic acid is used as a negative plate additive in the manufacture of lead-acid batteries. It is added at the rate of 0.6 g per kg of the oxide while preparing the paste. It is believed to enhance the ...
Hydrogenation of an unsaturated fatty acid refers to the addition of hydrogen atoms to the acid, causing double bonds to become single ones, as carbon atoms acquire new hydrogen partners (to maintain four bonds per carbon atom). Full hydrogenation results in a molecule containing the maximum amount of hydrogen (in other words, the conversion of an unsaturated fatty acid into a saturated one). Partial hydrogenation results in the addition of hydrogen atoms at some of the empty positions, with a corresponding reduction in the number of double bonds. Typical commercial hydrogenation is partial to obtain a malleable mixture of fats that is solid at room temperature, but melts during baking, or consumption. In most naturally occurring unsaturated fatty acids, the hydrogen atoms are on the same side of the double bonds of the carbon chain (cis configuration - from the Latin, meaning "on the same side"). However, partial hydrogenation reconfigures most of the double bonds that do not become chemically ...
Both COX1 and COX2 (also termed prostaglandin-endoperoxide synthase-1 (PTGS1) and PTGS2, respectively) metabolize arachidonic acid by adding molecular O2 between carbons 9 and 11 to form an endoperoxide bridge between these two carbons, adding molecular O2 to carbon 15 to yield a 15-hydroperoxy product, creating a carbon-carbon bond between carbons 8 and 12 to create a cyclopentane ring in the middle of the fatty acid, and in the process making PGG2, a product that has two fewer double bonds than arachidonic acid. The 15-hydroperoxy residue of PGG2 is then reduced to a 15-hydroxyl residue thereby forming PGH2. PGH2 is the parent prostanoid to all other prostanoids. It is metabolized by (see diagram in Prostanoids: a) the Prostaglandin E synthase pathway in which any one of three isozymes, PTGES, PTGES2, or PTGES3, convert PGH2 to PGE2 (subsequent products of this pathway include PGA2 and PGB2 (see Prostanoid#Biosynthesis); b) PGF synthase which converts PGH2 to PGF2α; c) Prostaglandin D2 ...
57-8. New ceramides from Acnistus arborescens J. Braz. Chem. Soc. Vol. 21 no. 5 pps. 867-871, 2010 Maia, Veras, Braz-Filho et ...
Part 1: Isolation and Structures of Novel Ceramides". Tetrahedron. 56 (51): 9895-9899. doi:10.1016/S0040-4020(00)00959-5. Nagao ...
"Antiapoptotic roles of ceramide-synthase-6-generated C16-ceramide via selective regulation of the ATF6/CHOP arm of ER-stress- ... Ceramide synthase 6 is a protein that in humans is encoded by the CERS6 gene. GRCh38: Ensembl release 89: ENSG00000172292 - ... "Entrez Gene: Ceramide synthase 6". Retrieved 2016-04-11. Senkal CE, Ponnusamy S, Bielawski J, Hannun YA, Ogretmen B (2010). " ...
Other names in common use include trihexosyl ceramide galactosidase, ceramide trihexosidase, ceramidetrihexoside alpha- ... Brady RO, Gal AE, Bradley RM, Martensson E (1967). "The metabolism of ceramide trihexosides. I. Purification and properties of ...
... but also as a substrate for ceramide synthase; aminopentol is acylated by ceramide synthase to form N-palmitoyl-AP1. This ... Inhibition of ceramide synthase is not responsible for this effect. The main factors could be DNA fragmentation and caspase-3 ... The inhibition of ceramide synthase by FB1 is thought to be reversible, since the binding is formed by noncovalent interactions ... N-palmitoyl-AP1 is an even more potent inhibitor of ceramide synthase and may therefore play a role in the toxicity of ...
Ceramides (N-acyl-sphingoid bases) are a major subclass of sphingoid base derivatives with an amide-linked fatty acid. The ... Hinkovska-Galcheva V, VanWay SM, Shanley TP, Kunkel RG (November 2008). "The role of sphingosine-1-phosphate and ceramide-1- ... The major phosphosphingolipids of mammals are sphingomyelins (ceramide phosphocholines),[40] whereas insects contain mainly ... a sphingolipid derived from ceramide that is a potent messenger molecule involved in regulating calcium mobilization,[74] cell ...
Haak D, Gable K, Beeler T, Dunn T (November 1997). "Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and ...
These ceramides are synthesized from the acylation of sphingosine. The biosynthetic pathway for sphingosine is found below: As ... Sphingolipids are formed from ceramides that consist of a fatty acid chain attached to the amino group of a sphingosine ...
Ceramide kinase, a lipid kinase that phosphorylates ceramides to ceramide-1-phosphate. G protein receptor kinases (GRK) of GRK2 ... Sugiura M, Kono K, Liu H, Shimizugawa T, Minekura H, Spiegel S, Kohama T (June 2002). "Ceramide kinase, a novel lipid kinase. ...
They possess ubiquinone 10 as their major respiratory quinone, contain glycosphingolipids (GSLs), specifically ceramide, ... "Bacterial ceramides and sphingophospholipids induce apoptosis of human leukaemic cells". Microbiology. 149 (8): 2071-2081. doi: ...
It may be phosphorylated by ceramide kinase to form ceramide-1-phosphate. Alternatively, it may be glycosylated by ... Dihydroceramides are then desaturated to form ceramide. De novo generated ceramide is the central hub of the sphingolipid ... Glycosphingolipids are ceramides with one or more sugar residues joined in a β-glycosidic linkage at the 1-hydroxyl position ( ... Ceramides, as a general class, are N-acylated sphingoid bases lacking additional head groups. Dihydroceramide is produced by N- ...
Ceramide transferase protein (or CERT) is responsible for the transfer of ceramide from the endoplasmic reticulum (ER) to the ... Hanada K (2010). "Intracellular trafficking of ceramide by ceramide transfer protein". Proceedings of the Japan Academy, Series ... The transport of ceramide by CERT requires ATP. CERT - when expressed in mammalian cells - has been found to receive a lot of ... This suggests that Ceramide is still transformed to Glycosylceramide at Golgi. Moreover, it has been shown that it does not ...
Dihydroceramide is then further desaturated to form ceramide. In addition, the breakdown of sphingolipids is also dependent on ... Particularly, the synthesis of ceramide requires PLP. In this reaction, serine is decarboxylated and combined with palmitoyl- ...
Yaoita Y, Satoh Y, Kikuchi M (2007). "A new ceramide from Ramaria botrytis (Pers.) Ricken". Journal of Natural Medicine. 61 (2 ... "A new ceramide from Suillus luteus and its cytotoxic activity against human melanoma cells". Chemistry and Biodiversity. 5 (1 ...
Ceramide glucosyltransferase is an enzyme that in humans is encoded by the UGCG gene. Glycosphingolipids (GSLs) are a group of ... 2001). "Role of ceramide in mediating the inhibition of telomerase activity in A549 human lung adenocarcinoma cells". J. Biol. ... Ichikawa S, Sakiyama H, Suzuki G, Hidari KI, Hirabayashi Y (Jul 1996). "Expression cloning of a cDNA for human ceramide ... 2005). "P-glycoprotein is implicated in the inhibition of ceramide-induced apoptosis in TF-1 acute myeloid leukemia cells by ...
The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene ... Perry DK, Hannun YA (December 1998). "The role of ceramide in cell signaling". Biochimica et Biophysica Acta (BBA) - Molecular ... "p53-Independent ceramide formation in human glioma cells during gamma-radiation-induced apoptosis". Cell Death and ...
ISBN 978-92-5-105157-3. Yaoita Y, Satoh Y, Kikuchi M (2007). "A new ceramide from Ramaria botrytis (Pers.) Ricken". Journal of ... in addition to the previously unknown ceramide (2S,2'R,3R,4E,8E)-N-2'-hydroxyoctadecanoyl-2-amino-9-methyl-4,8-heptade-cadiene- ...
They found increased levels of ceramide in the CSF, and it led to oxidative stress and bioenergetic failure in the neurons of ... "Cerebrospinal fluid ceramides from patients with multiple sclerosis impair neuronal bioenergetics". Brain. 137 (8): 2271-2286. ...
11) identified relationships between dihydroceramides, the precursor to ceramides, that revealed increased rates of ceramide ... Ceramide is upregulated and associated with mortality in patients with chronic heart failure. Can J Cardiol 2015;31:357-363pmid ... Ceramide synthase 5 mediates lipid-induced autophagy and hypertrophy in cardiomyocytes. J Clin Invest 2012;122:3919-3930pmid: ... Plasma ceramides are elevated in female children and adolescents with type 2 diabetes. J Pediatr Endocrinol Metab 2013;26:995- ...
Ceramides - HAPPI is the industrys leading magazine covering the global personal care, household and industrial and ...
A rescue cream infused with six rare African oils and a ceramide complex for intense moisture and replenishing barrier support ... Ceramide AP, Ceramide EOP,Sodium Hyaluronate Crosspolymer, Ceramide NP, Phytosphingosine, Ceteareth-20, Trisodium ... Ceramides AP, EOP, and NP: Help soothe and maintain the moisture balance of skin while protecting against water loss. - Sodium ... Ceramides AP, EOP, and NP: Help soothe and maintain the moisture balance of skin while protecting against water loss. -Sodium ...
Ceramides are a family of waxy lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in ... Ceramide VI is the most abundant ceramide of the skin, along with ceramide II, and has been exploited to model the organization ... The word ceramide comes from the Latin cera (wax) and amide. Ceramide is a component of vernix caseosa, the waxy or cheese-like ... Additionally, ceramides are being explored as a potential therapeutic in cancer. Ceramide is rarely found in bacteria. Bacteria ...
Using a product containing ceramides replenishes the ceramides in our skin that we have lost over time or due to environmental ... A few of our favorite products that contain ceramides are Elizabeth Arden Ceramide Time Complex Moisture Cream, Kinerase Pro ... As we age, ceramides are also depleted from the skin and our skin can become drier and lose that smooth healthy glow of our ... Ceramides are naturally occurring lipids found in the skin and play a critical role in the barrier function of our skin. If our ...
What is a ceramide? It is a type of lipid found in the cell membrane of cells. The richest concentration of ceramides are found ... The support provided by ceramides keeps skin firm. However, as we age ceramides decline and skin begins to sag and wrinkle.. ... There are a few causes for the loss of ceramides as we age: Harsh cleansers disrupt and strip ceramides and lipids. Medications ... Dryness, flakiness, irritation, sensitivity, and redness are side effects of ceramide depletion.. In addition, ceramides are ...
EWGs Skin Deep® database gives you practical solutions to protect yourself and your family from everyday exposures to chemicals in personal care products.
This pro-ceramide moisturizer strengthens skins moisture barrier for plump-looking, baby-soft skin. ... Shop SUNDAY RILEYs Ice Ceramide Moisturizing Cream at Sephora. ... Ceramide NP, Ceramide EOP, Ceramide AP, Sodium Hyaluronate, ... Ceramides: Help form the skins natural moisture barrier. - Vitamin F: Replenishes lipid levels and strengthens the skins ... Ceramides: Help form the skins natural moisture barrier. -Vitamin F: Replenishes lipid levels and strengthens the skins ...
Ceramides are naturally occurring, long chains of fats that are major components of skins outer layers. Adding them to a ... On a skincare product ingredient label, youll see those listed as ceramide AP, ceramide EOP, ceramide NG, ceramide NP, ... ceramide NS, phytosphingosine, and sphingosine. The ceramides used in skincare products typically are derived from plants ( ... CLINICAL Ceramide-Enriched Firming Moisturizer. CALM Redness Relief Repairing Serum. About the Experts. Paula Begoun is the ...
Their experiments suggest that ceramide activates protein kinase C ζ(PKC-ζ) and causes association of PKC-ζ with SAPK and also ... The authors conclude that PKC-ζ mediates effects of ceramide to cause formation of a multiprotein complex that leads to ... Bourbon, N.A., Yun, J., and Kester, M. (2000) Ceramide directly activates protein kinase C to regulate a stress-activated ... Treatment of cells with the inflammatory cytokine interleukin 1 causes synthesis of ceramide, a sphingolipid metabolite that ...
Ceramide+glucosyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Ceramide glucosyltransferase (or glucosylceramide synthase) is a glucosyltransferase enzyme involved in the production of ... "Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of ...
Shop our community curated line of eye serums with ceramides, including Medik8 Pretox Eyelift at Truth in Aging. Order today to ... I was hunting around for products with ceramides and came across your review. I enjoyed re-reading this and being reminded of ... Dermaxyl is a patented oligopeptide and ceramide blend that smoothes wrinkles and repairs age-related skin damage. It works by ...
Measuring concentrations of a class of lipids known as ceramides in the blood may hold the key to helping clinicians identify ... All cells have the ability to make ceramides; however, ceramide levels tend to accumulate in the blood when we have too much ... Total cholesterol also increased with rising ceramide scores and males were less likely to have high levels of ceramides. ... Levels of ceramides in the blood help predict cardiovascular events Researchers say new marker can help identify patients at ...
Read on to learn how else your skin might benefit, who needs ceramides most, how to choose the right products, and more. ... ceramides help create a barrier to prevent permeability. This locks moisture into your skin, preventing it from becoming dry ... What are ceramides?. Ceramides are a class of fatty acids called lipids. Theyre naturally found in skin cells and make up ... As long as ceramides are replenished, your skin can benefit. If youre looking for a more "natural" way to induce ceramide ...
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in ... We have identified a differential role for ceramide kinase in mast-cell activation Title: A differential role for ceramide ... Title: Role of cytosolic phospholipase A(2)α in cell rounding and cytotoxicity induced by ceramide-1-phosphate via ceramide ... CERK ceramide kinase [Homo sapiens] CERK ceramide kinase [Homo sapiens]. Gene ID:64781 ...
Buy Too Cool For School Coconut Ceramide Mask at JCPenney.com today and enjoy great savings. Available Online Only! ... Lauric acid in coconut water instantly moisturizes and brightens skin, while ceramides protect skin with a healthy moisture ... Formulated with 30 percent coconut water and ceramides, this sheet mask quickly recharges skin with deep and refreshing ... Lightweight and translucent sheet material maximizes delivery of active ingredients in the coconut-ceramide serum into skin, ...
Ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high ... Ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high ... Mechanism by which ceramide signaling occurs. It is hypothesized that after ceramide is generated in the lipid bilayer in the ... Pathways for ceramide synthesis. There are two known means of generating ceramide. The sphingomyelinase pathway uses an enzyme ...
Mario Badescu Ceramide Herbal Eye Cream is an easily absorbed, non-greasy eye cream made with a blend of vegetable, carnation ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I had a small sample of this and most samples I dont end up buying ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I love this stuff. I have normal/combo skin and this is the kind of ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I started a new skincare regimen that cause the area around my eyes to ...
The focus of this book is the metabolism of ceramide in normal and diseased states and the biophysical and biochemical ... In this book many aspects of ceramide and sphingolipid biology are addressed. ... Many aspects of ceramide and sphingolipid biology are addressed in this book. Its focus is the metabolism of ceramide in normal ... Metabolism of ceramides and their metabolites is also focused with ceramide synthase family of proteins being a target of ...
Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Dadsena, S., Bockelmann, S., Mina, J. G. M., Hassan, D. G., Korneev, S ... Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. ... Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ... Ceramides bind VDAC2 to trigger mitochondrialapoptosis Final publishers version, 2 MB, PDF-document ...
Skeletal muscle ceramides and other sphingolipids.. Total ceramide content was higher (P , 0.01) in the skeletal muscle of OBS ... Ceramides and other sphingolipids in vastus lateralis. Total, saturated, and unsaturated ceramide content (A), sphingosine and ... Analysis of DAGs, ceramides, and sphingolipids.. Quantification of intramuscular DAGs, ceramides, and sphingolipids was ... 14) found that exercise training tended to reduce both the total ceramide content and the saturated ceramide species in obese ...
In this column, we will briefly review the science of ceramides and how they improve agi ... Ceramides are the major component of the skins surface. They protect against moisture loss to keep skin youthful, supple and ... It appears as ceramide PC-102 (Hydroxypropyl bislauramide MEA), ceramide PC-104, ceramide PC-108, ceramide 1, 2, 3, 111, 6-11, ... Unfortunately, synthetic ceramides do not penetrate the skin as well as natural ceramides. Ceramide penetration improves ...
Restore brings the original efficacy of ceramides into a multifunctional powder. ... Orgasol® Restore - Multifunctional powder with replenishing ceramides. Product range. Designed to impart sensory appeal, ... Together with cholesterol and saturated fatty acids, ceramides are essential components of the epidermis. They help prevent ... Restore brings the original efficacy of ceramides into a multifunctional powder. ...
Browse our complete collection of ceramide products. Elizabeth Ardens ceramides for facial skin help retain moisture to reduce ... Ceramides. Crucial to maintaining your moisture barrier, skins first defense against aging signs. ...
Homo sapiens mRNA for ceramide glucosyltransferase, complete cds. P. BC038711.1. Homo sapiens UDP-glucose ceramide ... ceramide glucosyltransferase-A. X. laevis. 94.4. 393. NP_001019378.1 * Conserved domains (CDD) * * Gene summary * * Protein ... PREDICTED: ceramide glucosyltransferase. M. mulatta. 100.0. 393. XP_003911414.1 * Conserved domains (CDD) * * Gene summary * * ... PREDICTED: ceramide glucosyltransferase. P. anubis. 100.0. 393. NP_003349.1 * Conserved domains (CDD) * * Gene summary * * ...
Ceramide Overnight Firming Mask. Ceramide Overnight Firming Mask is rated 4.7 out of 5 by 15. ... Rated 5 out of 5 by JdaS from absolutely love it Ive been using ceramide and prevage products for years and have received ... Rated 5 out of 5 by Deb33 from Ceramide Overnight Firming Mask This product gives my skin a refreshing and relaxing feeling. It ... In combination with your other ceramide products I have gotten rid of the redness and tightness in my inner cheeks that always ...
His team has since uncovered mounting evidence that bad ceramide translates to bad astrocytes because shorter ceramide cannot ... We think by manipulating ceramide metabolism, creating the right ceramide so to speak, we can reinstall ciliogenesis, ... Good ceramides in skin creams and shampoos may hold promise for Alzheimers treatment. *Download PDF Copy ... He also wants to identify what he believes are the different sets of proteins long and short ceramide bind to. The ultimate ...
However, the mechanisms of acid sphingomyelinase/ceramide-mediated dea ... Acid sphingomyelinase-induced ceramide release has been shown by many studies to induce apoptosis in response to various ... Inhibition of PKB/Akt1 by C2-ceramide involves activation of ceramide-activated protein phosphatase in PC12 cells. Mol Cell ... Basu S, Kolesnick R (1998) Stress signals for apoptosis: ceramide and c-Jun kinase. Oncogene 17:3277-3285CrossRefPubMedGoogle ...
Nine subclasses of ceramides have been described in human SC; these differ from each other by the sphingoid base groups ( ... Ceramide content and changes of composition are involved in agingand skin diseases such as atopic dermatitis (AD),5-7 psoriasis ... Ceramides are the main polar lipids of the SC intercellular lipids; they play an important role in skin barrier function and ... Ceramides in Healthy and Diseased Skin. October 23, 2008 , Contact Author , By: Myeong Jun Choi and Howard I. Maibach ...
... by Dr. Trupti Shirole on August 13, 2016 at 5:58 PM Heart ... Plasma ceramides are a class of lipids that are highly linked to cardiovascular disease processes. Mayo Clinic has launched a ... "Plasma ceramides are promising biomarkers for the prediction of adverse CV events in either primary and/or secondary prevention ... A new blood test will measures blood concentrations of plasma ceramides to predict adverse cardiovascular events in patients ...
  • There has been research suggesting that when ionizing radiation causes apoptosis in some cells, the radiation leads to the activation of sphingomyelinase in the cell membrane and ultimately, to ceramide generation. (wikipedia.org)
  • As a bioactive lipid, ceramide has been implicated in a variety of physiological functions including apoptosis, cell growth arrest, differentiation, cell senescence, cell migration and adhesion. (wikipedia.org)
  • The most well-known functions of ceramides as cellular signals include regulating the differentiation , proliferation , programmed cell death (PCD), and apoptosis (Type I PCD) of cells . (bionity.com)
  • Ceramide accumulation due to induction of the de novo pathway has been implicated in chemotherapy-induced apoptosis of some cancer cells. (bionity.com)
  • Ceramide was first implicated in being a signal for programmed cell death when patients with the genetic disorder Niemann-Pick Disease were found to have certain cell types that were resistant to apoptosis. (bionity.com)
  • Over time, series of experiments at multiple institutions have shown that ceramide is a pro-apoptotic signal that, itself, can induce apoptosis in many cell types. (bionity.com)
  • Currently, research is being done on the role that ceramide plays in apoptosis that is induced by ionizing radiation . (bionity.com)
  • Unlike VDAC1 removal, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon cancer cells largely resistant to ceramide-induced apoptosis. (rug.nl)
  • Acid sphingomyelinase-induced ceramide release has been shown by many studies to induce apoptosis in response to various stimuli. (springer.com)
  • Dumitru CA, Gulbins E (2006) TRAIL activates acid sphingomyelinase via a redox mechanism and releases ceramide to trigger apoptosis. (springer.com)
  • These ceramides affect the molecules through various mechanisms and ultimately lead to apoptosis (programmed cell death) and heart muscle protein instability. (medindia.net)
  • Recent evidence indicates that SCC depletion is not exclusively a result of DNA damage but is critically coupled to ceramide-induced endothelial cell apoptosis within the mucosal microvascular network. (jci.org)
  • Possible role in ceramide biosynthesis, apoptosis and anticancer potential. (abcam.com)
  • Ceramide modulates a number of biochemical and cellular responses to stress, including apoptosis, cell-cycle arrest and cell senescence. (nih.gov)
  • Molecular mechanisms of the involvement of ceramide in apoptosis in pancreatic β-cells and its role in the formation of insulin resistance in pathogenesis of type 2 diabetes are reviewed. (springer.com)
  • Evidence also supports important role of ceramides in neuronal apoptosis. (hindawi.com)
  • This review summarizes recent findings related to the role of ceramides in oxidative stress-driven neuronal apoptosis and interplay with A β in the cascade of events ending in neuronal degeneration. (hindawi.com)
  • Multiple experimental approaches suggest an important role for ceramide in regulating such diverse responses as cell cycle arrest, apoptosis, and cell senescence. (sciencemag.org)
  • Ceramide is a building block for complex sphingolipids in the plasma membrane, but it also plays a significant role in secondary signalling pathways regulating cell proliferation and apoptosis in response to stress. (hindawi.com)
  • Ceramide mediates controlled cell death by triggering several signalling cascades to initiate caspase-dependent and independent apoptosis [ 3 ]. (hindawi.com)
  • Ceramide and the induction of apoptosis. (aacrjournals.org)
  • The central molecule ceramide triggers apoptosis of pulmonary epithelial cells, and increased pulmonary levels of ceramide lead to the development of emphysema in mice [ 5 , 7 ]. (ersjournals.com)
  • The elevated CerS6 activity resulted in accumulation of the pro-apoptotic C16 : 0 ceramide, which facilitates the mitochondrial apoptosis in response to oxidative stress. (nih.gov)
  • Hydrolytic pathway protects against ceramide-induced apoptosis in keratinocytes exposed to UVB. (biomedsearch.com)
  • Although ceramides (Cers) are key constituents of the epidermal permeability barrier, they also function as apoptogenic signals for UVB irradiation-induced apoptosis in epidermal keratinocytes. (biomedsearch.com)
  • Ceramide plays an important role in different biological processes such as cell signaling, proliferation, differentiation and apoptosis. (doria.fi)
  • Particularly, the role of ceramides in apoptosis has received special attention in recent years. (doria.fi)
  • A few of our favorite products that contain ceramides are Elizabeth Arden Ceramide Time Complex Moisture Cream, Kinerase Pro Therapy Ultra Rich Night Repair, and Topix SRS Pro-Ceramide Barrier Repair. (skinstore.com)
  • Since 1990, Elizabeth Arden has marketed a variety of expensive ceramide products as "anti-aging. (happi.com)
  • I love the Elizabeth Arden ceramide products and find that they do actually work with my daughter often being taken for a friend or sister - much to her annoyance! (debenhams.com)
  • Take away the signs of ageing with Elizabeth Arden Ceramide Premiere Regeneration Eye Cream. (hqhair.com)
  • Elizabeth Arden Ceramide Premiere Regeneration Eye Cream uses skin-replenishing ceramides, nutrient-rich sea minerals, and high-performance retinols to help support your skin's own natural collagen to leave it looking refreshed, hydrated and radiant. (hqhair.com)
  • Elizabeth Arden Ceramide Replenishing Cleansing Oil r en forts ttning p Ceramide-serien (som skyddar hudbarri ren) och inneh ller de uppmjukande, antioxidantiska och v rdande egenskaperna fr n aprikos, tistel och olivolja. (bangerhead.se)
  • Aggregation of LDL cholesterol by ceramide causes LDL retention in arterial walls, leading to atherosclerosis. (wikipedia.org)
  • Ceramides work in conjunction with lipids like cholesterol, fatty acids, and squalene to provide a critical layer of protection for healthy skin. (skinstore.com)
  • Skin inhibits water movement and controls loss via its structure, which has a unique composition of 50% ceramides, 25% cholesterol, and 15% free fatty acids. (paulaschoice.com)
  • Ceramides work best for skin when they're combined with other replenishing ingredients like fatty acids and cholesterol. (paulaschoice.com)
  • Although previous research conducted outside the U.S. has shown elevated ceramide levels among people with confirmed heart disease or post-heart attack, this is the first study to show its predictive power among people with no blockages and in those with low levels of low-density lipoprotein (LDL), the so-called "bad" cholesterol. (eurekalert.org)
  • In fact, individuals with the highest levels of blood ceramides were found to have a threefold to fourfold greater risk of suffering a cardiovascular event compared with those with the lowest ceramide score, regardless of their LDL cholesterol level or the presence of a blockage in the heart's arteries. (eurekalert.org)
  • Meeusen said that unlike cholesterol, which is fairly inert, acting like a clog in the arteries, ceramides play an active role in the cardiovascular disease process by attracting and drawing inflammatory cells and promoting clotting. (eurekalert.org)
  • for each one-point increase in the ceramide risk score, the risk rose by 9 percent--a trend that remained even after fully adjusting for other risk factors including age, sex, high blood pressure, smoking, total cholesterol, HDL and markers of inflammation. (eurekalert.org)
  • Total cholesterol also increased with rising ceramide scores and males were less likely to have high levels of ceramides. (eurekalert.org)
  • The stratum corneum requires ceramides, cholesterol and fatty acids to provide the cutaneous permeability barrier. (cosmeticsandtoiletries.com)
  • The stratum corneum generates enzymes, which convert phospholipids and other lipids into a mixture of ceramides, cholesterol and free fatty acids. (happi.com)
  • ceramides, fatty acids and cholesterol. (happi.com)
  • There are basically four stratum corneum-modifying cosmeceuticals: ceramides, cholesterol, urea and lactic acid. (happi.com)
  • Together with cholesterol and saturated fatty acids, ceramides are essential components of the epidermis. (arkema.com)
  • This layer is predominantly composed of dead skin cells-corneocytes-embedded in a rich lipid matrix composed of ceramides, cholesterol, and free fatty acids. (aiche.org)
  • To understand the role of cholesterol, mixtures of ceramides and cholesterol in a bilayer configuration are examined and compared to pure ceramide bilayers. (aiche.org)
  • Furthermore, examination of the hydrogen bonding network in the bilayer reveals a reduction in ceramide-ceramide hydrogen bonding due to the presence of cholesterol. (aiche.org)
  • Ceramides-lipids that accumulate in tissues during hyperlipidemia and inflammation-are significantly associated with increased cardiovascular disease events, even among individuals with low low-density lipoprotein cholesterol (LDL-C) levels and no evidence of coronary stenosis. (aacc.org)
  • Meeusen and his colleagues followed for a median of 5.9 years 499 patients who had been referred for coronary angiography, assessing not only ceramides levels but also LDL-C, high-density lipoprotein cholesterol, triglycerides, C-reactive protein, and lipoprotein-associated phospholipase A2. (aacc.org)
  • Ceramides form stable bilayer structures when complexed with cholesterol phosphocholine (CholPC), a large-headgroup sterol. (doria.fi)
  • In a paper just published in the inaugural issue of the new journal, Alzheimer's & Dementia , Claudio Costantini, Rekha Kolasani, and Luigi Puglielli argue that the lipids ceramide and cholesterol may provide metabolic links between AD and aging. (alzforum.org)
  • Furthermore, many metabolic pathways may be shared by ceramide and cholesterol, further complicating interpretation of their specific roles in aging and disease. (alzforum.org)
  • Luigi, do you want to start us off with a brief statement about the role of ceramide and cholesterol and what issues we might address today? (alzforum.org)
  • I would expect questions on metabolism and molecular/biochemical roles of cholesterol and ceramide, and their role (if any) in aging and AD. (alzforum.org)
  • We have also investigated the effect of hydrogen bonding between sphingomyelin (SM) and ceramides or cholesterol interactions. (doria.fi)
  • Much of the complexity in the cellular sphingolipid pool derives from the large number of acyl chains that can be incorporated into the ceramide scaffold. (diabetesjournals.org)
  • Treatment of cells with the inflammatory cytokine interleukin 1 causes synthesis of ceramide, a sphingolipid metabolite that mediates a signal that causes growth arrest. (sciencemag.org)
  • CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. (nih.gov)
  • Many aspects of ceramide and sphingolipid biology are addressed in this book. (springer.com)
  • Use in sphingolipid ceramide studies and as internal standard. (abcam.com)
  • Ceramide is a pro-apoptotic sphingolipid with unique physical characteristics. (uni-muenchen.de)
  • Ceramides, the major molecules of sphingolipid metabolism and lipid second messengers, have been associated with AD progression and pathology via A β generation. (hindawi.com)
  • Sphingolipid ceramide N -deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N -acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. (clontech.com)
  • We report now that methamphetamine accelerates cellular senescence and activates transcription of genes involved in cell-cycle control and inflammation by stimulating production of the sphingolipid messenger ceramide. (sigmaaldrich.com)
  • Skin ceramide alterations in first-episode schizophrenia indicate abnormal sphingolipid metabolism. (biomedsearch.com)
  • Researchers measured four different types of ceramides in the blood at baseline and combined the values into a 12-point scale. (eurekalert.org)
  • There are nine types of ceramides in the stratum corneum, conveniently named ceramide 1 through ceramide 9, and they account for 40 to 50% of the lipids in this outermost layer. (happi.com)
  • One of several types of ceramides, which are naturally occurring, long chains of lipids (fats) that are major components of skin's outer layers. (paulaschoice.com)
  • A Dr. Jart representative tells us that there are different types of ceramides found in the skin, all of which have slightly different functions and therefore using products with multiples types can be beneficial. (thezoereport.com)
  • Ceramides are a major component of our skin's surface. (skinstore.com)
  • Ceramides: Help form the skin's natural moisture barrier. (sephora.com)
  • This rich but lightweight dual action moisturizer uses beetroot and coconut extract to pull hydration into the skin, while ceramides, vitamin F, and pomegranate sterols strengthen the skin's natural moisture barrier sealing in moisture. (sephora.com)
  • It's unclear whether your skin's naturally occurring ceramide levels relate to your risk of developing certain underlying skin conditions. (healthline.com)
  • Via this route, it is claimed that ceramides can reach the skin's lower cells and upper layers to support elastin and collagen production to keep skin firm, perform their role in renewing skin cells from the inside out and integrate between the cells of the stratum corneum to keep the skin hydrated. (happi.com)
  • Helps to replenish skin's essential ceramides to reinforce skin's protective barrier. (elizabetharden.com)
  • SkinCeuticals ceramides are formulated synthetically to be bioidentical to skin's natural ceramides. (skinceuticals.com)
  • Ceramides help hold healthy cells together, which keeps the skin's moisture level balanced and optimally hydrated for dewy, smooth, and soothed skin," Dr. Lain explains. (thezoereport.com)
  • Ceramides play an important part of your skin's role as a protective barrier, helping to bind together the cells that make up the surface of your skin.These nourishing oils help retain moisture and protect your skin. (victoriahealth.com)
  • Ceramide glucosyltransferase (or glucosylceramide synthase) is a glucosyltransferase enzyme involved in the production of glucocerebroside. (wikipedia.org)
  • Ichikawa S, Sakiyama H, Suzuki G, Hidari KI, Hirabayashi Y: Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis. (drugbank.ca)
  • Contrary to previous assumptions that ceramides and other sphingolipids found in cell membrane were purely supporting structural elements, ceramide can participate in a variety of cellular signaling: examples include regulating differentiation, proliferation, and programmed cell death (PCD) of cells. (wikipedia.org)
  • Third, in the "salvage" pathway, sphingolipids that are broken down into sphingosine are reused by reacylation to form ceramide. (wikipedia.org)
  • Once in the Golgi apparatus, ceramide can be further metabolized to other sphingolipids, such as sphingomyelin and the complex glycosphingolipids. (wikipedia.org)
  • Ceramides are central intermediates in the biosynthetic pathway that produces the large family of sphingolipids, which includes more than 4,000 distinct molecular entities. (diabetesjournals.org)
  • For years, it was assumed that ceramides and other sphingolipids found in the bilayer cell membrane were purely structural elements. (bionity.com)
  • This book is about the various roles of bioactive ceramides and other sphingolipids in cellular biology. (springer.com)
  • Ceramide 1-phosphate and other sphingolipids are also presented in cellular physiology and pathophysiology. (springer.com)
  • When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner. (uniprot.org)
  • This review presents data on the functional biochemistry of ceramide, one of the key sphingolipids with properties of a secondary messenger. (springer.com)
  • Mutants lacking SIT4 or KTI12 display a shift towards nonhydroxylated forms of long chain bases and sphingolipids, suggesting regulation of hydroxylase ( SUR2 ) or ceramide synthase by Sit4p. (hindawi.com)
  • Ceramide is a building block for complex sphingolipids which comprise an important structural component of the plasma membrane. (hindawi.com)
  • Many of the genes involved are conserved from yeast to higher eukaryotes, with diversion in the synthesis of complex sphingolipids occurring only after the production of ceramides, resulting in the production of different end products in the pathway. (hindawi.com)
  • Ceramide belongs to an important class of sphingolipids and it constitutes the hydrophobic backbone of all complex sphingolipids. (doria.fi)
  • A rescue cream infused with six rare African oils and a ceramide complex for intense moisture and replenishing barrier support. (sephora.com)
  • We highly suggest First Aid Beauty Ultra Repair Cream that incorporates ceramides with shea butter and colloidal oatmeal to produce an amazing calming, smoothing, hydrating cream that reduce symptoms of eczema, dermatitis, and keratosis pilaris. (skinstore.com)
  • For example, if you have dry skin, consider a ceramide-containing cream. (healthline.com)
  • Receive a Free Full Size Ceramide Lift and Firm Night Cream with any $125.00 Purchase (A $80 Value! (shopstyle.com)
  • SkinMedica TNS Ceramide Treatment Cream™ moisturizes, reduces wrinkles, repairs skin damage and increases thickness of the skin. (skincarerx.com)
  • This ultra-rich cream is infused with Ceramides and Rose Oil to deeply hydrate the skin for a healthier, more supple appearance and feel. (pixibeauty.com)
  • Invigorate your skin with Pure Creations' Elasticity Boost Eye Cream with Ceramides. (communitynaturalfoods.com)
  • First, the sphingomyelinase pathway uses an enzyme to break down sphingomyelin in the cell membrane and release ceramide. (wikipedia.org)
  • Second, the de novo pathway creates ceramide from less complex molecules. (wikipedia.org)
  • The salvage pathway re-utilizes long-chain sphingoid bases to form ceramide through the action of ceramide synthase. (wikipedia.org)
  • analyzed the signaling connection between ceramide and the SAPK pathway in human embryonic kidney cells. (sciencemag.org)
  • Title: Identification of an acid sphingomyelinase ceramide kinase pathway in the regulation of the chemokine CCL5. (nih.gov)
  • Regardless of which pathway is used, the generation of pools of ceramide serve to signal the cell towards a programmed cell death. (bionity.com)
  • De Novo pathway - catalyzed by the enzyme ceramide synthase. (bionity.com)
  • Glioma cell death induced by irradiation or alkylating agent chemotherapy is independent of the intrinsic ceramide pathway. (abcam.com)
  • Genetic and expression analyses indicate that ceramide is required for maintaining the proper expression of previously characterized starvation-responsive genes, genes that are regulated by the IIS pathway and tumor suppressor Rb, and genes responsive to pathogen. (genetics.org)
  • This pathogenic cascade is triggered by reactive oxygen species, likely generated through methamphetamine metabolism via cytochrome P450, and involves the recruitment of nuclear factor-κB (NF-κB) to induce expression of enzymes in the de novo pathway of ceramide biosynthesis. (sigmaaldrich.com)
  • I wonder, Luigi, (pardon my ignorance) if there are enzymes in the ceramide synthesis pathway that are inducible? (alzforum.org)
  • Ceramides are a family of waxy lipid molecules. (wikipedia.org)
  • As children and young adults, our bodies manufacture plenty of ceramide molecules to keep our skin healthy, supple, and strong. (skinstore.com)
  • Ceramides are made up of long-chain fatty acids that link with other important molecules to promote cellular function. (healthline.com)
  • This is an amino acid chain that includes ceramide as one of its molecules. (healthline.com)
  • Ceramides are a family of lipid molecules. (bionity.com)
  • Its focus is the metabolism of ceramide in normal and diseased states and the biophysical and biochemical mechanisms governing the bioactivity of these molecules. (springer.com)
  • For example, if this interaction between Unc45 and ceramide is preventing Unc45 from performing its normal maintenance, then screening for small molecules that block that interaction could be an avenue to find therapeutics that help Unc45 maintain its role in optimizing muscle protein function. (medindia.net)
  • Ceramides are waxy lipid molecules naturally present in the stratum corneum, the outermost layer of the skin, that play a key role in skin lipid barrier function. (skinceuticals.com)
  • Ceramides are found in high concentrations within the cell membrane of eukaryotic cells, since they are component lipids that make up sphingomyelin, one of the major lipids in the lipid bilayer. (wikipedia.org)
  • Because sphingomyelin is one of the four common phospholipids found in the plasma membrane of cells, the implications of this method of generating ceramide is that the cellular membrane is the target of extracellular signals leading to programmed cell death. (wikipedia.org)
  • As a positive feedback loop, the generated oligomeric and fibrillar A β induces a further increase in ceramide levels by activating sphingomyelinases that catalyze the catabolic breakdown of sphingomyelin to ceramide. (hindawi.com)
  • In the sphingomyelin cycle, a number of extracellular agents and insults (such as tumor necrosis factor, Fas ligands, and chemotherapeutic agents) cause the activation of sphingomyelinases, which act on membrane sphingomyelin and release ceramide. (sciencemag.org)
  • The addition of inositol to ceramide in yeast forms inositol phosphoceramide, and glucose, galactose or phosphorylcholine is added to ceramide in mammalian cells to generate glycosphingolipids and sphingomyelin [ 9 ]. (hindawi.com)
  • However, we need to keep in mind that ceramide (as a second messenger) is generated by hydrolysis of cell-surface sphingomyelin. (alzforum.org)
  • Your skin goes into natural repair mode while you sleep - Ceramide Overnight Firming Mask is formulated to optimally support this process. (elizabetharden.com)
  • A ceramide is composed of sphingosine and a fatty acid. (wikipedia.org)
  • Ceramide can be further hydrolyzed by acid ceramidase to form sphingosine and a free fatty acid, both of which are able to leave the lysosome, unlike ceramide. (wikipedia.org)
  • Diet that lacks essential fatty acids disrupts the levels of ceramides and lipids. (skinstore.com)
  • Ceramides are a class of fatty acids called lipids. (healthline.com)
  • Although human skin is naturally made up of ceramides, these fatty acids are lost over time. (healthline.com)
  • Similar to fatty acids, ceramides are a type of lipids found in cell membranes. (lorealparisusa.com)
  • We found, through genetic manipulation and lipid analysis, that biosynthesis of ceramide, particularly those with longer fatty acid side chains, critically impacts animal survival during L1 diapause. (genetics.org)
  • Harnessing the powers of Ceramides and Cupuaçu Butter (rich in fatty acids), the nourishing formula locks in moisture without greasy or sticky residue. (thehut.com)
  • The richest concentration of ceramides are found in the epidermis. (skinstore.com)
  • Medications alter ceramide and lipid levels in the epidermis. (skinstore.com)
  • Clinical studies have shown that people with such skin conditions naturally produce fewer ceramides in the epidermis compared to people with normal skin. (skinstore.com)
  • A high concentration of ceramides is located in the epidermis- the outermost layer of skin cells. (lorealparisusa.com)
  • Here we demonstrate that ceramide synthase 4 (CerS4) is highly expressed in the epidermis of adult mice where it is localized in the interfollicular epidermis and defined populations within the pilosebaceous unit. (sigmaaldrich.com)
  • The present study demonstrates that treatment of glioma cells with clinically achievable doses of gemcitabine results in acid sphingomyelinase activation, lysosomal accumulation of ceramide, cathepsin D activation, Bax insertion into the mitochondria, and cell death. (springer.com)
  • Our data indicate a critical role of the acid sphingomyelinase/ceramide system for gemcitabine-induced signaling and suggest that lysosomal ceramide accumulation mediates cell death induced by a chemotherapeutic drug. (springer.com)
  • Lipotoxic cardiomyopathy (LCM) is caused due to the accumulation of ceramides. (medindia.net)
  • Lipotoxic cardiomyopathy (LCM) is a heart condition that usually occurs in patients with diabetes and obesity due to the accumulation of a type of fat known as ceramides, finds a study conducted at Sanford Burnham Prebys Medical Discovery Institute (SBP). (medindia.net)
  • Lipotoxic cardiomyopathy (LCM) is caused by the accumulation of ceramides, which occurs due to the consumption of a diet high in fat or high in calories. (medindia.net)
  • Several extracellular agents and stress stimuli, such as tumour necrosis factor alpha, chemotherapeutic agents and heat, cause ceramide accumulation. (nih.gov)
  • This spectrum of inducers of ceramide accumulation and the nature of ceramide-mediated responses suggest that ceramide is a key component of intracellular stress response pathways. (sciencemag.org)
  • Tired skin is refreshed as ceramides and hyaluronic acid-both powerful, water-binding agents-replenish hydration and help smooth the appearance of fine lines to encourage more youthful-looking under-eyes. (ulta.com)
  • Hyaluronic Acid delivers plumping and firming water-based hydration deep below the surface of the skin for and Ceramide lock in that nourishment , reducing moisture loss. (adorebeauty.com.au)
  • This wonderful blend of ceramides, hyaluronic acid and shea butter promotes elasticity while treating dehydrated and inflamed skin. (communitynaturalfoods.com)
  • Although everyone waxes poetic about the benefits of hyaluronic acid for hydration, ceramides are the lesser known ingredient that deserve a place in your beauty arsenal. (thezoereport.com)
  • Although our skin naturally produces ceramides too - just like hyaluronic acid and collagen - they also start to deplete as we age. (thezoereport.com)
  • Collagen30 with Ceramides is a natural anti-aging powerhouse featuring Verisol Bioactive Collagen Peptides, Ceramosides phytoceramide extract, hyaluronic acid, vitamin C, and biotin. (jeancoutu.com)
  • We have used Drosophila as a model to show that elevated levels of ceramide --driven by genetic or dietary manipulation-- is sufficient to induce LCM," says Rolf Bodmer, Ph.D., professor and director of the Development, Aging and Regeneration Program at SBP and senior author of the paper. (medindia.net)
  • suggest that this rise in pH increases susceptibility to lung infection by altering levels of ceramide, a membrane constituent that can also trigger cell death. (sciencemag.org)
  • Certain cancer cells have shown an increased level of ceramides and with the help of these protein interactions, the progression and establishment of cancer can be analyzed. (medindia.net)
  • Using products with ceramides can also greatly help the treatment of eczema. (skinstore.com)
  • Using products with ceramides is proven to provide considerable relief for these conditions. (skinstore.com)
  • Blocking the biosynthesis of ceramide via acid sphingomyelinase normalizes ceramide levels and, most tellingly, renders the Cftr -deficient mice resistant to lung infections. (sciencemag.org)
  • Tom, as far as I know the enzymes involved with the biosynthesis of ceramide are not inducible. (alzforum.org)
  • The authors conclude that PKC-ζ mediates effects of ceramide to cause formation of a multiprotein complex that leads to activation of SAPK. (sciencemag.org)
  • This site includes a membrane-buried glutamate that mediates direct contact with the ceramide head group. (rug.nl)
  • In the case of glycosphingolipids, exohydrolases acting at acidic pH optima cause the stepwise release of monosaccharide units from the end of the oligosaccharide chains, leaving just the sphingosine portion of the molecule, which may then contribute to the generation of ceramides. (wikipedia.org)
  • Serum ceramides have emerged as potential biomarkers of insulin resistance, diabetes, and heart disease. (diabetesjournals.org)
  • These studies are consistent with earlier, smaller studies evaluating relationships between serum ceramides and insulin resistance in both humans and nonhuman primates ( 6 - 10 ). (diabetesjournals.org)
  • Abbreviated summary of published studies relating serum ceramides to various measures of cardiometabolic disease. (diabetesjournals.org)
  • Lightweight and translucent sheet material maximizes delivery of active ingredients in the coconut-ceramide serum into skin, and provides the most comfortable sheet-masking experience. (jcpenney.com)
  • Elizabeth Arden's Retinol Ceramide Capsules Line Erasing Night Serum are a targeted treatment to visibly diminish lines and wrinkles while improving skin texture and tone. (refinery29.com)
  • Niemann-Pick Disease is a disease in which there is a deficiency in acid sphingomyelinase, the enzyme that leads to the production of ceramide. (bionity.com)
  • However, the mechanisms of acid sphingomyelinase/ceramide-mediated death signaling following treatment with chemotherapeutic drugs have not been fully elucidated thus far. (springer.com)
  • Along those lines, would it be possible to speculate on the potential downside of inhibition of ceramide synthesis or sphingomyelinase? (alzforum.org)
  • Ceramides are naturally occurring lipids found in the skin and play a critical role in the barrier function of our skin. (skinstore.com)
  • A pro-ceramide moisturizer that strengthens the natural moisture barrier against environmental stressors for plump-looking, baby-soft skin. (sephora.com)
  • Ceramides help create a barrier to prevent permeability. (healthline.com)
  • Although more research is needed, there's reason to believe that using ceramide-containing skin care products may help soothe related irritation and provide an additional barrier to certain cases of dry skin. (healthline.com)
  • Lauric acid in coconut water instantly moisturizes and brightens skin, while ceramides protect skin with a healthy moisture barrier. (jcpenney.com)
  • As a way of counteracting this age-related pattern, ceramide products may work to improve skin barrier function and maintain skin-hydration. (lorealparisusa.com)
  • Ceramides are important to skin because they are an essential part of the lipid barrier. (happi.com)
  • Kao claims that its ceramide-based Curel products replenish and repair skin by forming a protective barrier. (happi.com)
  • CeraVe claims its ceramide-based product helps repair, replenish and protect the skin barrier to leave skin looking and feeling soft, clean and healthy. (happi.com)
  • Ceramides work synergistically to help increase skin hydration and promote optimal barrier function. (skinceuticals.com)
  • A highly enriched rice ceramide ampoule locks in moisture for 24 hours and strengthen skin protective barrier. (iherb.com)
  • A lightweight formulated rice ceramide instantly absorbed into the skin layers and enhances a protective barrier which keeps future irritants out. (iherb.com)
  • The innovative anti-ageing capsules directly deliver a concentrated dosage of bio-engineered, skin-identical ceramides, helping to repair and strengthen the lipid barrier as well as support natural collagen renewal, resulting in a re-texturising and plumping effect to radically minimise the appearance of fine lines. (hqhair.com)
  • Hydroxypropyl bispalmitamide MEA (ceramide) acts as a skin barrier agent that promotes and maintains the moisture mantle of the skin and improves the skin texture by emollient and moisture-retaining effects. (skincarerx.com)
  • Ceramides are crucial for skin barrier function, but little is known about the regulation of epidermal appendages and whether stem cell populations that control their regeneration depend on specific ceramide species. (sigmaaldrich.com)
  • Ceramides are a naturally occurring type of lipid, or fat, that comprise an extremely important part of the outer layer of the skin, known as the skin barrier. (thezoereport.com)
  • In other words, ceramides assist in helping your skin barrier perform at its best. (thezoereport.com)
  • Incorporating ceramides into a skin care regime not only helps to strengthen the skin barrier, making it more resilient but also helps to comfort and soothe any irritation. (thezoereport.com)
  • Using more than one ceramide enables the creation of a stronger skin barrier that means more moisture is retained within the skin. (thezoereport.com)
  • If the benefits of this skin savior weren't enough, ceramides also work wonders for hair too, helping to strengthen our tresses in the same say it rebuilds our skin barrier. (thezoereport.com)
  • Ceramides induce skeletal muscle insulin resistance when synthesized as a result of saturated fat activation of TLR4 receptors. (wikipedia.org)
  • Ceramides induce insulin resistance in many tissues by inhibition of Akt/PKB signaling. (wikipedia.org)
  • With the analytical power that derives from profiling such a large number of samples obtained at two visits, 5 years apart, the data from the Strong Heart Family Study (SHFS) revealed that several ceramide species correlated with hyperinsulinemia and the HOMA of insulin resistance (HOMA-IR) in this at-risk population. (diabetesjournals.org)
  • Over the past 20 years, a large number of studies in preclinical models suggest that ceramides may be among the most pathogenic nutrient metabolites that accumulate in obesity, linking overnutrition to insulin resistance and its sequelae of comorbidities. (diabetesjournals.org)
  • Those participants with twofold higher (90th percentile) ceramide with 16:0 (Cer-16), Cer-18, Cer-20, or Cer-22 displayed hyperinsulinemia and insulin resistance (estimated using HOMA-IR, a measure of insulin resistance determined from fasting glucose and insulin concentrations). (diabetesjournals.org)
  • Our data also provide additional evidence in humans linking ceramides to insulin resistance. (diabetesjournals.org)
  • One of the main predispositions for the development of insulin resistance and diabetes is obesity, which is associated with ectopic fat deposition and significant increase in intracellular concentrations of cytotoxic ceramides. (springer.com)
  • We've found that ceramide has a big effect on insulin resistance. (fightaging.org)
  • Ceramides AP, EOP, and NP: Help soothe and maintain the moisture balance of skin while protecting against water loss. (sephora.com)
  • The good news is that by replenishing lost ceramides, skin is able to fortify itself and retain optimal moisture for a firmer, smoother look. (happi.com)
  • Intensive overnight moisture repair formula with 'Ceramide' plumping technology. (debenhams.com)
  • Naturally found in the cuticle and therefore responsible for hair smoothness, moisture retention and elasticity, our own ceramides deplete from excess heat, chemical processes such as coloring and over-washing. (thezoereport.com)
  • Ideal for the hands and feet, OPI Prospa Moisture Bonding Ceramide Spray infuses dry skin with long-lasting hydration. (thehut.com)
  • Ceramide thus acts as an intermediary signal that connects the external signal to the internal metabolism of the cells. (bionity.com)
  • Metabolism of ceramides and their metabolites is also focused with ceramide synthase family of proteins being a target of extensive review. (springer.com)
  • We think by manipulating ceramide metabolism, creating the right ceramide so to speak, we can reinstall ciliogenesis,' Bieberich said. (news-medical.net)
  • Formulated with 30 percent coconut water and ceramides, this sheet mask quickly recharges skin with deep and refreshing hydration, leaving skin dewy and healthy-looking. (jcpenney.com)
  • Electrochemical analysis revealed significantly improved hydration in the ceramide group as well. (victoriahealth.com)
  • Clinically shown to reduce fine lines around the eyes and deep wrinkles, Collagen30 with Ceramides also significantly improves skin hydration and elasticity in 15 days. (jeancoutu.com)
  • Fructis Grow Strong Conditioner, with Apple Extract and Ceramide, fortifies hair as it grows to bring life back to every inch: stronger, healthier, & shinier. (fredmeyer.com)
  • In a double-blind, placebo-controlled trial, healthy women took 350 mg of ceramide extract once a day for 12 weeks. (victoriahealth.com)
  • Here we find that sit4 Δ mutants have decreased ceramide levels and display resistance to exogenous ceramides and phytosphingosine. (hindawi.com)
  • In contrast, the phosphorylated ceramide precursors dihydrosphingosine (DHSP) and phytosphingosine (PHSP) are signals for pathways that promote cell proliferation [ 4 ]. (hindawi.com)
  • The long-chain sphingoid bases released from the lysosome may then re-enter pathways for synthesis of ceramide and/or sphingosine-1-phosphate. (wikipedia.org)
  • Thus, ceramide synthase family members probably trap free sphingosine released from the lysosome at the surface of the endoplasmic reticulum or in endoplasmic reticulum-associated membranes. (wikipedia.org)
  • Ceramide may also appear in products as sphingosine. (healthline.com)
  • Can ceramides be combined with other skin care ingredients for maximum effect? (healthline.com)
  • Using ceramides in combination with other skin care ingredients may help you better achieve your desired results. (healthline.com)
  • Ceramides are expensive ingredients and are usually found in higher-end skin care lines. (happi.com)
  • Ceramides are great team players that play beautifully with a whole host of other skin care ingredients and can be found in everything from cleansers to night creams. (thezoereport.com)
  • Ceramides are found in high concentrations within the cell membrane of cells. (bionity.com)
  • Perhaps one of the most fascinating aspects of ceramide is the fact that it can be released from the cell membrane by enzymes and then act as a signaling molecule. (bionity.com)
  • It is hypothesized that after ceramide is generated in the lipid bilayer in the plasma membrane in response to one of the aforementioned stress signals, ceramide clusters onto lipid platforms known as lipid rafts . (bionity.com)
  • Ceramide has also been shown to form organized large channels traversing the mitochondrial outer membrane. (bionity.com)
  • In addition, it is been suggested that the membrane biophysical properties are affected upon generations of ceramide. (doria.fi)
  • Therefore, ceramide-related membrane phenomena have become a subject of great interest both in cellular and in model membrane studies. (doria.fi)
  • This thesis focuses on investigating how specific structural details of ceramides influence their interactions with other lipids present in the membrane bilayers. (doria.fi)
  • Overall, the results of this thesis showed how the gelphase forming properties of ceramides are influenced by their molecular structure and the colipids present in the membrane bilayers, which may have distinct effects on cell physiology. (doria.fi)
  • Ceramides cause endothelial dysfunction by activating protein phosphatase 2 (PP2A). (wikipedia.org)
  • In vitro, ceramide activates a serine-threonine protein phosphatase, and in cells it regulates protein phosphorylation as well as multiple downstream targets [such as interleukin converting enzyme (ICE)-like proteases, stress-activated protein kinases, and the retinoblastoma gene product] that mediate its distinct cellular effects. (sciencemag.org)
  • Ceramide activated protein phosphatase activity has been previously observed in association with the Sit4 protein phosphatase. (hindawi.com)
  • ELISA Kits detecting Ceramide synthase 1 can be used to research brain development or to study mental retardation. (biocompare.com)
  • Ceramides make up almost half of the lipids found in the Stratum Corneum (outer most layer of skin). (glymedplus.com)
  • What's the difference between synthetic and natural ceramides? (healthline.com)
  • The only "natural" ceramides are the ones already in your skin. (healthline.com)
  • But natural ceramides are very unstable substances and very costly to obtain, so synthetic ceramides are frequently used. (happi.com)
  • Unfortunately, synthetic ceramides do not penetrate the skin as well as natural ceramides. (happi.com)
  • Ceramides play an important part in cellular signaling such as regulatory differentiation, proliferation and programmed cell death. (happi.com)
  • Although it is known that the cellular response to ceramide is important for the regulation of cell proliferation and cell death pathways, the precise molecular mechanisms for this regulation still remain elusive. (hindawi.com)
  • Methamphetamine accelerates cellular senescence through stimulation of de novo ceramide biosynthesis. (sigmaaldrich.com)
  • Ceramides are important lipids that are implicated in several cellular functions. (doria.fi)
  • Measuring concentrations of a class of lipids known as ceramides in the blood may hold the key to helping clinicians identify individuals with suspected coronary heart disease who need treatment or should be followed more closely, according to research scheduled for presentation at the American College of Cardiology's 66th Annual Scientific Session. (eurekalert.org)
  • Plasma ceramides are a class of lipids that are highly linked to cardiovascular disease processes. (medindia.net)
  • Here we show that ceramide generated on the surface of endothelium coalesces to form ceramide-rich platforms that transmit an apoptotic signal. (jci.org)
  • where we show that ceramide levels increase during aging in the brain of wild-type animals but not in p75NTR knockout animals. (alzforum.org)
  • Inhibitory effects of ceramide kinase on Rac1 activation, lamellipodium formation, cell migration, and metastasis of A549 lung cancer cells. (nih.gov)
  • The inhibitor simple prevented the age-associated increase in ceramide and Aß levels in WT animals. (alzforum.org)
  • A sialodiosylceramide consisting of the trisaccharide α-Neu5Ac-(2→3)-β- D -Gal-(1→4)-β- D -Glc attached to the primary hydroxy function of ceramide. (ebi.ac.uk)
  • De novo synthesis of ceramide begins with the condensation of palmitate and serine to form 3-keto-dihydrosphingosine. (wikipedia.org)
  • De novo synthesis of ceramide occurs in the endoplasmic reticulum. (wikipedia.org)
  • Their experiments suggest that ceramide activates protein kinase C ζ(PKC-ζ) and causes association of PKC-ζ with SAPK and also with other members of the SAPK-activating MAP kinase cascade. (sciencemag.org)
  • People who have certain skin conditions may be more likely to benefit from ceramide supplements, as these treat the underlying condition from the inside out. (healthline.com)
  • And many subjects reported healthy-looking skin eight weeks after the study's end, demonstrating a lasting benefit from ceramide supplementation. (victoriahealth.com)
  • The hydrating benefits of ceramides in skin care are most often found in moisturizers, facial cleansers, eye creams, sunscreens, lipsticks, and foundations. (lorealparisusa.com)
  • Still, many companies are touting the benefits of ceramides. (happi.com)
  • Clinical studies have shown promising benefits of ceramides in treating atopic dermatitis. (happi.com)
  • The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. (rsc.org)
  • Here, the properties of ceramide bilayers are examined using models derived from the CHARMM36 forcefield [1] with missing parameters and charges determined from density functional theory calculations [2]. (aiche.org)
  • There are three major pathways of ceramide generation. (wikipedia.org)
  • Ceramides may initiate a cascade of biochemical alterations, which ultimately leads to neuronal death by diverse mechanisms, including depolarization and permeabilization of mitochondria, increased production of reactive oxygen species (ROS), cytochrome c release, Bcl-2 depletion, and caspase-3 activation, mainly by modulating intracellular signalling, particularly along the pathways related to Akt/PKB kinase and mitogen-activated protein kinases (MAPKs). (hindawi.com)
  • Genetic interaction analysis suggests that ceramide may act in both insulin-IGF-1 signaling (IIS)-dependent and IIS-independent pathways to affect starvation survival. (genetics.org)
  • As ceramides are a naturally occurring component of healthy skin, all skin types can use them. (thezoereport.com)
  • Ceramide production naturally decelerates but we can also lose them from daily, habitual activities like washing our hands, taking a shower, or laying in the sun. (thezoereport.com)
  • The ceramides naturally present in our skin give it that young, supple appearance. (victoriahealth.com)
  • But ceramide levels naturally decrease as we age - making it hard to maintain smooth, supple, healthy skin as we get older. (victoriahealth.com)
  • Mayo Clinic has launched a new type of blood test that will measures blood concentrations of plasma ceramides to predict adverse cardiovascular events in patients with progressing coronary artery disease (CAD). (medindia.net)
  • Risk conferred by plasma ceramides appears to be independent of other established and novel biomarkers, and there are preliminary indications that high ceramide concentrations can be modified by common lipid-lowering therapies. (medindia.net)
  • Plasma ceramide concentrations were measured on a liquid chromatography tandem mass spectrometry metabolomics platform. (unboundmedicine.com)
  • Our study documented a novel positive association between baseline plasma ceramide concentrations and incident CVD. (unboundmedicine.com)
  • In addition, a Mediterranean dietary intervention may mitigate potential deleterious effects of elevated plasma ceramide concentrations on CVD. (unboundmedicine.com)
  • In some animal models, ceramide inhibition led to an almost complete restoration of insulin sensitivity. (fightaging.org)
  • We hypothesized that chronically exercise-trained muscle would be characterized by lower skeletal muscle diacylglycerols (DAGs) and ceramides despite higher IMTGs and would account for its higher insulin sensitivity. (diabetesjournals.org)
  • In cultured cells or isolated tissues, ceramides inhibit insulin signaling and action and inhibit lipid oxidation ( 2 ). (diabetesjournals.org)
  • Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells. (nih.gov)
  • It is interesting to note that the substances that can cause ceramide to be generated tend to be stress signals that can cause the cells to go into programmed cell death. (bionity.com)
  • Ceramides serve as the glue that locks together the surface area of skin cells. (happi.com)
  • A long version of waxy, fat-like ceramide appears critical to helping brain cells called astrocytes stay focused on supporting neurons rather than contributing to their demise. (news-medical.net)
  • While still uncertain what determines which ceramide gets made, right now he wants to better understand just how the lipid regulates cilia on brain cells and how dysregulation leads to problems such as chronic inflammation and eventually diseases such as Alzheimer's. (news-medical.net)
  • Moreover, we report the generation of 2A2, an anti-ceramide monoclonal antibody that binds to ceramide to prevent platform formation on the surface of irradiated endothelial cells of the murine GI tract. (jci.org)
  • Cells were stimulated with ( B ) 10 Gy for indicated times or ( C ) increasing radiation doses for 1 minute and stained with anti-ceramide antibody, and platforms were identified as in A . ( D and E ) BAECs were pretreated with anti-ceramide MID 15B4 (1 μg/ml). (jci.org)
  • Ceramide, a pro-cell death lipid, kills cancer cells by causing them to eat their own mitochondria," said Ogretmen. (eurekalert.org)
  • Patient cells with the FLT3 mutation inhibit ceramide synthesis and thereby become resistant to cell death. (eurekalert.org)
  • By adding a synthetic ceramide analogue, LCL-461, the researchers were able to reactivate mitophagy and kill drug-resistant AML cells in a dish. (eurekalert.org)
  • In contrast to LDL-C's artery-clogging properties, ceramides actively attract inflammatory cells and promote clotting, Meeusen explained in a prepared statement. (aacc.org)
  • We also find that the Sit4p target Elongator is not involved in the ceramide response but that cells deficient in Kti12p-an accessory protein with an undefined regulatory role-have similar ceramide phenotypes to sit4 Δ mutants. (hindawi.com)
  • Additionally, we have developed and studied a novel method of introducing ceramides to cells, using a solvent-free approach. (doria.fi)
  • By adding ceramide/CholPC complexes to the growth medium, various chain length ceramides were successfully delivered to cells in culture. (doria.fi)
  • The rate of uptake also determined how the cells metabolised the ceramides. (doria.fi)
  • As cells age, for example, ceramide levels rise in vivo, and the lipid is further increased by about threefold in the brains of AD patients. (alzforum.org)
  • Ceramide is a component of vernix caseosa, the waxy or cheese-like white substance found coating the skin of newborn human infants. (wikipedia.org)
  • As such, we suggest that 2A2 represents a prototype of a new class of anti-ceramide therapeutics and an effective countermeasure against radiation GI syndrome mortality. (jci.org)
  • If you're looking for a more "natural" way to induce ceramide production in your skin, consider adding healthy fats to your diet. (healthline.com)
  • However, research does suggest that people who have eczema or psoriasis have fewer ceramides in their skin. (healthline.com)
  • Plasma ceramides are promising biomarkers for the prediction of adverse CV events in either primary and/or secondary prevention. (medindia.net)
  • Although in vitro studies and investigations in animal models and small clinical populations have suggested that ceramides may represent an intermediate link between overnutrition and certain pathological mechanisms underlying cardiovascular disease (CVD), no prospective studies have investigated the association between plasma ceramides and risk of CVD. (unboundmedicine.com)
  • The ultimate goals of Bieberich and his research team include restoring production of healthy ceramide and cilia in the face of these diseases. (news-medical.net)
  • Despite the strongly consistent findings obtained in preclinical models, the role of ceramides in human cardiometabolic pathologies has been controversial. (diabetesjournals.org)
  • Ceramides play an important role. (skinstore.com)
  • While ceramides are noted for their role in brain and nervous system development, they've gained a lot of interest in the skin care world for their potential skin health benefits. (healthline.com)
  • A role for ceramide kinase. (nih.gov)
  • Ceramide biosynthesis in keratinocyte and its role in skin function. (lorealparisusa.com)
  • Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity. (rug.nl)
  • Ceramides play a complicated role in the heart. (medindia.net)
  • It proved that ceramides play a central role in LCM. (medindia.net)
  • In the Blood article, Ogretmen and his colleagues, including clinicians at the MD Anderson Cancer Center who provided patient samples, report that ceramide-dependent mitophagy plays a key role in chemotherapeutic-mediated AML cell death. (eurekalert.org)
  • Therefore, Kti12p may play a similar secondary role in the ceramide response. (hindawi.com)
  • Ceramide-1-phosphate (C1P) plays an important role in several biological processes, being identified as a key regulator of many protein functions. (beilstein-journals.org)