Ceramides: Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE.Sphingolipids: A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)Sphingomyelins: A class of sphingolipids found largely in the brain and other nervous tissue. They contain phosphocholine or phosphoethanolamine as their polar head group so therefore are the only sphingolipids classified as PHOSPHOLIPIDS.Glucosylceramides: Cerebrosides which contain as their polar head group a glucose moiety bound in glycosidic linkage to the hydroxyl group of ceramides. Their accumulation in tissue, due to a defect in beta-glucosidase, is the cause of Gaucher's disease.Alkaline Ceramidase: A ceramidase subtype that is active at alkaline pH. It is found at high levels within the SMALL INTESTINE.Sphingosine: An amino alcohol with a long unsaturated hydrocarbon chain. Sphingosine and its derivative sphinganine are the major bases of the sphingolipids in mammals. (Dorland, 28th ed)Sphingomyelin Phosphodiesterase: An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12.Cerebrosides: Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)Sphingosine N-Acyltransferase: An enzyme that catalyzes the acyltransferase of SPHINGOSINE to N-acylsphingosine using acyl-COENZYME A as donor and COENZYME A as acceptor. The enzyme is mainly localized in the MITOCHONDRIA.Ceramidases: Amidohydrolases that are specific for the cleavage of the N-acyl linkage of CERAMIDES. Ceramidases are classified as acidic, neutral or basic according to the optimal pH with which they function.Serine C-Palmitoyltransferase: A key enzyme in SPHINGOLIPIDS biosynthesis, this enzyme catalyzes the pyridoxal-5'-phosphate-dependent condensation of L-SERINE and PALMITOYL COENZYME A to 3-dehydro-D-sphinganine. The enzyme consists of two different subunits.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Glycosphingolipids: Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)Epidermis: The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).I Blood-Group System: A blood group related both to the ABO and P systems that includes several different antigens found in most people on erythrocytes, in milk, and in saliva. The antibodies react only at low temperatures.Chromatography, Gas: Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.Water Loss, Insensible: Loss of water by diffusion through the skin and by evaporation from the respiratory tract.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Lipidoses: Conditions characterized by abnormal lipid deposition due to disturbance in lipid metabolism, such as hereditary diseases involving lysosomal enzymes required for lipid breakdown. They are classified either by the enzyme defect or by the type of lipid involved.Fumonisins: A group of MYCOTOXINS found in CORN contaminated with FUSARIUM fungus. They are chains of about 20 carbons with acidic ester, acetylamino and sometimes other substituents. They inhibit ceramide synthetase conversion of SPHINGOLIPIDS to CERAMIDES.Acid Ceramidase: A ceramidase subtype that is active at acid pH. It plays an important role in sphingolipid degradation by catalyzing the lysosomal hydrolysis of ceramide to sphingosine and free fatty acid. Inherited deficiency of acid ceramidase activity results in FARBER LIPOGRANULOMATOSIS.Neutral Glycosphingolipids: A subclass of GLYCOSPHINGOLIPIDS containing one or more sugars within their head group connected directly to a ceramide moiety. They consist of monoglycosyl-, and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Sulfoglycosphingolipids: GLYCOSPHINGOLIPIDS with a sulfate group esterified to one of the sugar groups.Hydroxy Acids: Organic compounds containing both the hydroxyl and carboxyl radicals.Lipid Metabolism: Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.Amino Alcohols: Compounds possessing both a hydroxyl (-OH) and an amino group (-NH2).Glycolipids: Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)Lysophospholipids: Derivatives of PHOSPHATIDIC ACIDS that lack one of its fatty acyl chains due to its hydrolytic removal.Mice, Hairless: Mutant strains of mice that produce little or no hair.G(M3) Ganglioside: A ganglioside present in abnormally large amounts in the brain and liver due to a deficient biosynthetic enzyme, G(M3):UDP-N-acetylgalactosaminyltransferase. Deficiency of this enzyme prevents the formation of G(M2) ganglioside from G(M3) ganglioside and is the cause of an anabolic sphingolipidosis.DiglyceridesGlucosylceramidase: A glycosidase that hydrolyzes a glucosylceramide to yield free ceramide plus glucose. Deficiency of this enzyme leads to abnormally high concentrations of glucosylceramide in the brain in GAUCHER DISEASE. EC 3.2.1.45.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Fatty Alcohols: Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Galactosylceramides: Cerebrosides which contain as their polar head group a galactose moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in beta-galactosidase, is the cause of galactosylceramide lipidosis or globoid cell leukodystrophy.Galactosylgalactosylglucosylceramidase: An enzyme that catalyzes the hydrolysis of a ceramidetrihexoside to a ceramidedihexoside plus galactose.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Spectrometry, Mass, Electrospray Ionization: A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.Neutral Ceramidase: A ceramidase subtype that is active at neutral pH. It is found at high levels within the SMALL INTESTINE and in the BRAIN.Lipid Bilayers: Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.Phospholipase D: An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.Lactosylceramides: Glycosphingolipids which contain as their polar head group a lactose moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in lactosylceramide beta-galactosidase, is the cause of lactosylceramidosis.Optical Rotation: The rotation of linearly polarized light as it passes through various media.Sphingolipidoses: A group of inherited metabolic disorders characterized by the intralysosomal accumulation of SPHINGOLIPIDS primarily in the CENTRAL NERVOUS SYSTEM and to a variable degree in the visceral organs. They are classified by the enzyme defect in the degradation pathway and the substrate accumulation (or storage). Clinical features vary in subtypes but neurodegeneration is a common sign.AmidohydrolasesSpectrophotometry, Infrared: Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Spectrometry, Mass, Fast Atom Bombardment: A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.Glucosyltransferases: Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Glycerophospholipids: Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.

SDZ PSC 833, the cyclosporine A analogue and multidrug resistance modulator, activates ceramide synthesis and increases vinblastine sensitivity in drug-sensitive and drug-resistant cancer cells. (1/2471)

Resistance to chemotherapy is the major cause of cancer treatment failure. Insight into the mechanism of action of agents that modulate multidrug resistance (MDR) is instrumental for the design of more effective treatment modalities. Here we show, using KB-V-1 MDR human epidermoid carcinoma cells and [3H]palmitic acid as metabolic tracer, that the MDR modulator SDZ PSC 833 (PSC 833) activates ceramide synthesis. In a short time course experiment, ceramide was generated as early as 15 min (40% increase) after the addition of PSC 833 (5.0 microM), and by 3 h, [3H]ceramide was >3-fold that of control cells. A 24-h dose-response experiment showed that at 1.0 and 10 microM PSC 833, ceramide levels were 2.5- and 13.6-fold higher, respectively, than in untreated cells. Concomitant with the increase in cellular ceramide was a progressive decrease in cell survival, suggesting that ceramide elicited a cytotoxic response. Analysis of DNA in cells treated with PSC 833 showed oligonucleosomal DNA fragmentation, characteristic of apoptosis. The inclusion of fumonisin B1, a ceramide synthase inhibitor, blocked PSC 833-induced ceramide generation. Assessment of ceramide mass by TLC lipid charring confirmed that PSC 833 markedly enhanced ceramide synthesis, not only in KB-V-1 cells but also in wild-type KB-3-1 cells. The capacity of PSC 833 to reverse drug resistance was demonstrated with vinblastine. Whereas each agent at a concentration of 1.0 microM reduced cell survival by approximately 20%, when PSC 833 and vinblastine were coadministered, cell viability fell to zero. In parallel experiments measuring ceramide metabolism, it was shown that the PSC 833/vinblastine combination synergistically increased cellular ceramide levels. Vinblastine toxicity, also intensified by PSC 833 in wild-type KB-3-1 cells, was as well accompanied by enhanced ceramide formation. These data demonstrate that PSC 833 has mechanisms of action in addition to P-glycoprotein chemotherapy efflux pumping.  (+info)

Mesalamine blocks tumor necrosis factor growth inhibition and nuclear factor kappaB activation in mouse colonocytes. (2/2471)

BACKGROUND & AIMS: Derivatives of 5-aminosalicylic acid (mesalamine) represent a mainstay in inflammatory bowel disease therapy, yet the precise mechanism of their therapeutic action is unknown. Because tumor necrosis factor (TNF)-alpha is important in the pathogenesis of inflammatory bowel disease, we investigated the effect of mesalamine on TNF-alpha-regulated signal transduction and proliferation in intestinal epithelial cells. METHODS: Young adult mouse colon cells were studied with TNF-alpha, epidermal growth factor, or ceramide in the presence or absence of mesalamine. Proliferation was studied by hemocytometry. Mitogen-activated protein (MAP) kinase activation and IkappaBalpha expression were determined by Western blot analysis. Nuclear transcription factor kappaB (NF-kappaB) nuclear translocation was determined by confocal laser immunofluorescent microscopy. RESULTS: The antiproliferative effects of TNF-alpha were blocked by mesalamine. TNF-alpha and ceramide activation of MAP kinase were inhibited by mesalamine, whereas epidermal growth factor activation of MAP kinase was unaffected. TNF-alpha-stimulated NF-kappaB activation and nuclear translocation and the degradation of Ikappa-Balpha were blocked by mesalamine. CONCLUSIONS: Mesalamine inhibits TNF-alpha-mediated effects on intestinal epithelial cell proliferation and activation of MAP kinase and NF-kappaB. Therefore, it may function as a therapeutic agent based on its ability to disrupt critical signal transduction events in the intestinal cell necessary for perpetuation of the chronic inflammatory state.  (+info)

Ceramide induces cytochrome c release from isolated mitochondria. Importance of mitochondrial redox state. (3/2471)

In the present study we show that N-acetylsphingosine (C2-ceramide), N-hexanoylsphingosine (C6-ceramide), and, to a much lesser extent, C2-dihydroceramide induce cytochrome c (cyto c) release from isolated rat liver mitochondria. Ceramide-induced cyto c release is prevented by preincubation of mitochondria with a low concentration (40 nM) of Bcl-2. The release takes place when cyto c is oxidized but not when it is reduced. Upon cyto c loss, mitochondrial oxygen consumption, mitochondrial transmembrane potential (Delta Psi), and Ca2+ retention are diminished. Incubation with Bcl-2 prevents, and addition of cyto c reverses the alteration of these mitochondrial functions. In ATP-energized mitochondria, ceramides do not alter Delta Psi, neither when cyto c is oxidized nor when it is reduced, ruling out a nonspecific disturbance by ceramides of mitochondrial membrane integrity. Furthermore, ceramides decrease the reducibility of cyto c. We conclude that the apoptogenic properties of ceramides are in part mediated via their interaction with mitochondrial cyto c followed by its release and that the redox state of cyto c influences its detachment by ceramide from the inner mitochondrial membrane.  (+info)

Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells. (4/2471)

LY-A strain is a Chinese hamster ovary cell mutant resistant to sphingomyelin (SM)-directed cytolysin and has a defect in de novo SM synthesis. Metabolic labeling experiments with radioactive serine, sphingosine, and choline showed that LY-A cells were defective in synthesis of SM from these precursors, but not syntheses of ceramide (Cer), glycosphingolipids, or phosphatidylcholine, indicating a specific defect in the conversion of Cer to SM in LY-A cells. In vitro experiments showed that the specific defect of SM formation in LY-A cells was not due to alterations in enzymatic activities responsible for SM synthesis or degradation. When cells were treated with brefeldin A, which causes fusion of the Golgi apparatus with the endoplasmic reticulum (ER), de novo SM synthesis in LY-A cells was restored to the wild-type level. Pulse-chase experiments with a fluorescent Cer analogue, N-(4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-pentanoyl)-D-erythro-sphingosine (C5-DMB-Cer), revealed that in wild-type cells C5-DMB-Cer was redistributed from intracellular membranes to the Golgi apparatus in an intracellular ATP-dependent manner, and that LY-A cells were defective in the energy-dependent redistribution of C5-DMB-Cer. Under ATP-depleted conditions, conversion of C5-DMB-Cer to C5-DMB-SM and of [3H]sphingosine to [3H]SM in wild-type cells decreased to the levels in LY-A cells, which were not affected by ATP depletion. ER-to-Golgi apparatus trafficking of glycosylphosphatidylinositol-anchored or membrane-spanning proteins in LY-A cells appeared to be normal. These results indicate that the predominant pathway of ER-to-Golgi apparatus trafficking of Cer for de novo SM synthesis is ATP dependent and that this pathway is almost completely impaired in LY-A cells. In addition, the specific defect of SM synthesis in LY-A cells suggests different pathways of Cer transport for glycosphingolipids versus SM synthesis.  (+info)

Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells. (5/2471)

Using the modified screening approach in combination with expressed sequence tags, we have identified several novel cDNAs from mouse embryonic stem (ES) cells, whose expression is tissue-restricted and/or developmentally regulated. One of the cDNAs, ES18, is preferentially expressed in lymph node and thymus, and contains noteworthy features of transcriptional regulator. The expression of ES18 transcript was selectively regulated during the apoptosis of T-cell thymoma S49.1 induced by several stimuli. Interestingly, the ES18 transcript was differently regulated in the mutually antagonistic process, between dexamethasone- and A23187-induced cell death of T-cells. Moreover, the message level of ES18 was selectively enhanced by staurosporine, a broad protein kinase inhibitor, but not by other protein kinase inhibitors such as GF109203X and H89. In addition, ES18 transcript was induced by C2-ceramide, which is a mediator of both dexamethasone- and staurosporine-induced apoptotic signaling. We further showed that transient overexpression of ES18 in mouse T-cell lymphoma increased the apoptotic cell death. These data suggest that ES18 may be selectively involved in specific apoptotic processes in mouse T-cells.  (+info)

Signal transduction triggered by lipid A-like molecules in 70Z/3 pre-B lymphocyte tumor cells. (6/2471)

The lipid A (endotoxin) moiety of lipopolysaccharide (LPS) elicits rapid cellular responses from many cell types, including macrophages, lymphocytes, and monocytes. In CD14 transfected 70Z/3 pre-B lymphocyte tumor cells, these responses include activation of the MAP kinase homolog, p38, activation of NF-kappaB, and transcription of kappa light chains, leading to the assembly of surface IgM. In this work, we explored the specificity of the response with regard to lipid structure, and the requirement for p38 kinase activity prior to NF-kappaB activation in control and CD14 transfected 70Z/3 (CD14-70Z/3) cells. A p38-specific inhibitor, SB203580, was used to block p38 kinase activity in cells. CD14-70Z/3 cells were incubated with 1-50 microM SB203580, and then stimulated with LPS. Nuclear extracts were prepared, and NF-kappaB activation was measured using an electrophoretic mobility shift assay. SB203580 did not inhibit LPS induced NF-kappaB activation. In addition, LPS failed to activate p38 tyrosine phosphorylation in 70Z/3 cells lacking CD14, in spite of rapid NF-kappaB activation and robust surface IgM production with appropriate higher doses of LPS. LPS stimulation of p38 phosphorylation, NF-kappaB activation, and surface IgM expression were all blocked completely by lipid A-like endotoxin antagonists whether or not CD14 was present. Acidic glycerophospholipids and ceramides did not mimic lipid A-like molecules either as agonists or antagonists in this system. Our data support the hypothesis that lipid A-mediated activation of cells requires stimulation of a putative lipid A sensor that is downstream of CD14, but upstream of p38 and NF-kappaB.  (+info)

Nitric oxide donors induce stress signaling via ceramide formation in rat renal mesangial cells. (7/2471)

Exogenous NO is able to trigger apoptosis of renal mesangial cells, and thus may contribute to acute lytic phases as well as to resolution of glomerulonephritis. However, the mechanism involved in these events is still unclear. We report here that chronic exposure of renal mesangial cells for 24 h to compounds releasing NO, including spermine-NO, (Z)-1-{N-methyl-N-[6-(N-methylammoniohexyl)amino]}diazen-1-ium-1, 2-diolate (MAHMA-NO), S-nitrosoglutathione (GS-NO), and S-nitroso-N-acetyl-D,L-penicillamine (SNAP) results in a potent and dose-dependent increase in the lipid signaling molecule ceramide. Time courses reveal that significant effects occur after 2-4 h of stimulation with NO donors and reach maximal levels after 24 h of stimulation. No acute (within minutes) ceramide production can be detected. When cells were stimulated with NO donors in the presence of phorbol ester, a direct activator of protein kinase C, both ceramide production and DNA fragmentation are completely abolished. Furthermore, addition of exogenous ceramide partially reversed the inhibitory effect of phorbol ester on apoptosis, thus suggesting a negative regulation of protein kinase C on ceramide formation and apoptosis. In contrast to exogenous NO, tumor necrosis factor (TNF)-alpha stimulates a very rapid and transient increase in ceramide levels within minutes but fails to induce the late-phase ceramide formation. Moreover, TNF fails to induce apoptosis in mesangial cells. Interestingly, NO and TNFalpha cause a chronic activation of acidic and neutral sphingomyelinases, the ceramide-generating enzymes, whereas acidic and neutral ceramidases, the ceramide-metabolizing enzymes, are inhibited by NO, but potently stimulated by TNFalpha. Furthermore, in the presence of an acidic ceramidase inhibitor, N-oleoylethanolamine, TNFalpha leads to a sustained accumulation of ceramide and in parallel induces DNA fragmentation. In summary, our data demonstrate that exogenous NO causes a chronic up-regulation of ceramide levels in mesangial cells by activating sphingomyelinases and concomitantly inhibiting ceramidases, and that particularly the late-phase of ceramide generation may be responsible for the further processing of a proapoptotic signal.  (+info)

TNF-alpha increases ceramide without inducing apoptosis in alveolar type II epithelial cells. (8/2471)

Ceramide is a bioactive lipid mediator that has been observed to induce apoptosis in vitro. The purpose of this study was to determine whether endogenous ceramide, generated in response to in vivo administration of tumor necrosis factor-alpha (TNF-alpha), increases apoptosis in primary rat alveolar type II epithelial cells. Intratracheal instillation of TNF-alpha (5 microgram) produced a decrease in sphingomyelin and activation of a neutral sphingomyelinase. These changes were associated with a significant increase in lung ceramide content. TNF-alpha concomitantly activated the p42/44 extracellular signal-related kinases and induced nuclear factor-kappaB activation in the lung. Hypodiploid nuclei studies revealed that intratracheal TNF-alpha did not increase type II cell apoptosis compared with that in control cells after isolation. A novel observation from separate in vitro studies demonstrated that type II cells undergo a gradual increase in apoptosis after time in culture, a process that was accelerated by exposure of cells to ultraviolet light. However, culture of cells with a cell-permeable ceramide, TNF-alpha, or a related ligand, anti-CD95, did not increase apoptosis above the control level. The results suggest that ceramide resulting from TNF-alpha activation of sphingomyelin hydrolysis might activate the mitogen-activated protein kinase and nuclear factor-kappaB pathways without increasing programmed cell death in type II cells.  (+info)

*Saturated fat

Recommendations to reduce or limit dietary intake of saturated fats are made by the World Health Organization,[72] American Heart Association,[6] Health Canada,[73] the US Department of Health and Human Services,[74] the UK Food Standards Agency,[75] the Australian Department of Health and Aging,[76] the Singapore Government Health Promotion Board,[77] the Indian Government Citizens Health Portal,[78] the New Zealand Ministry of Health,[79] the Food and Drugs Board Ghana,[80] the Republic of Guyana Ministry of Health,[81] and Hong Kong's Centre for Food Safety.[82] In 2003, the World Health Organization (WHO) and Food and Agriculture Organization (FAO) expert consultation report concluded that "intake of saturated fatty acids is directly related to cardiovascular risk.[83] The traditional target is to restrict the intake of saturated fatty acids to less than 10% of daily energy intake and less than 7% for high-risk groups. If populations are consuming less than 10%, they should not increase that ...

*Monoglyceride

... s are primarily used as surfactants, usually in the form of emulsifiers. Together with diglycerides, monoglycerides are commonly added to commercial food products in small quantities as E471, which helps to prevent mixtures of oils and water from separating. The values given in the nutritional labels for total fat, saturated fat, and trans fat do not include those present in mono- and diglycerides as fats are defined as being triglycerides. They are also often found in bakery products, beverages, ice cream, chewing gum, shortening, whipped toppings, margarine, and confections.[5] In bakery products, monoglycerides are useful in improving loaf volume and texture, and as antistaling agents.[6][7] Monoglycerides are used to enhance the physical stability towards creaming in milk beverages.[8] ...

*Diglyceride

Ceramide (CAPPs, KSR1, PKCζ, CTSD). *Glucosylceramides. *S1P. *Sphingosine (SDK1, PKH, YPK) ...

*Globotriaosylceramide

It is also known as CD77, Gb3, and ceramide trihexoside.[2] It is one of the few clusters of differentiation that is not a ... "The role of ceramide trihexoside (globotriaosylceramide) in the diagnosis and follow-up of the efficacy of treatment of Fabry ...

*Omega-3 fatty acid

Evidence in the population generally does not support a beneficial role for omega−3 fatty acid supplementation in preventing cardiovascular disease (including myocardial infarction and sudden cardiac death) or stroke.[7][19][20][21][needs update] A 2018 meta-analysis found no support that daily intake of one gram of omega-3 fatty acid in individuals with a history of coronary heart disease prevents fatal coronary heart disease, nonfatal myocardial infarction or any other vascular event.[9] However, omega−3 fatty acid supplementation greater than one gram daily for at least a year may be protective against cardiac death, sudden death, and myocardial infarction in people who have a history of cardiovascular disease.[22] No protective effect against the development of stroke or all-cause mortality was seen in this population.[22] Eating a diet high in fish that contain long chain omega−3 fatty acids does appear to decrease the risk of stroke.[23] Fish oil supplementation has not been shown to ...

*Triacetin

... and two types of acetooleins have been found to be without toxic effects in long-term feeding tests in rats at levels that were several orders of magnitude greater than those to which consumers are exposed. Three types of acetostearins have been found to be without toxic effects in long-term feeding tests in rats at levels up to 5 g per kg per day. This contrasts with an estimated human consumption of a fraction of a milligram per kg per day. It is recognized that at an even higher feeding level (10 g per kg per day) male rats developed testicular atrophy and female rats, uterine discoloration. However, such a level which would amount to 50 g or more for an infant and 600 g for an adult per day, is vastly higher than would be possible in the consumption of foods to which acetostearins are added for functional purposes. ...

*Stearic acid

Being inexpensive and chemically benign, stearic acid finds many niche applications. As an example, it can be used to make castings from a plaster piece mold or waste mold, and to make a mold from a shellacked clay original. In this use, powdered stearic acid is mixed in water and the suspension is brushed onto the surface to be parted after casting. This reacts with the calcium in the plaster to form a thin layer of calcium stearate, which functions as a release agent. When reacted with zinc it forms zinc stearate, which is used as a lubricant for playing cards (fanning powder) to ensure a smooth motion when fanning. Stearic acid is a common lubricant during injection molding and pressing of ceramic powders.[12] It is also used as a mold release for foam latex that is baked in stone molds. Stearic acid is used as a negative plate additive in the manufacture of lead-acid batteries. It is added at the rate of 0.6 g per kg of the oxide while preparing the paste. It is believed to enhance the ...

*Trans fat

Hydrogenation of an unsaturated fatty acid refers to the addition of hydrogen atoms to the acid, causing double bonds to become single ones, as carbon atoms acquire new hydrogen partners (to maintain four bonds per carbon atom). Full hydrogenation results in a molecule containing the maximum amount of hydrogen (in other words, the conversion of an unsaturated fatty acid into a saturated one). Partial hydrogenation results in the addition of hydrogen atoms at some of the empty positions, with a corresponding reduction in the number of double bonds. Typical commercial hydrogenation is partial to obtain a malleable mixture of fats that is solid at room temperature, but melts during baking, or consumption. In most naturally occurring unsaturated fatty acids, the hydrogen atoms are on the same side of the double bonds of the carbon chain (cis configuration - from the Latin, meaning "on the same side"). However, partial hydrogenation reconfigures most of the double bonds that do not become chemically ...

*Eicosanoid

Both COX1 and COX2 (also termed prostaglandin-endoperoxide synthase-1 (PTGS1) and PTGS2, respectively) metabolize arachidonic acid by adding molecular O2 between carbons 9 and 11 to form an endoperoxide bridge between these two carbons, adding molecular O2 to carbon 15 to yield a 15-hydroperoxy product, creating a carbon-carbon bond between carbons 8 and 12 to create a cyclopentane ring in the middle of the fatty acid, and in the process making PGG2, a product that has two fewer double bonds than arachidonic acid. The 15-hydroperoxy residue of PGG2 is then reduced to a 15-hydroxyl residue thereby forming PGH2. PGH2 is the parent prostanoid to all other prostanoids. It is metabolized by (see diagram in Prostanoids: a) the Prostaglandin E synthase pathway in which any one of three isozymes, PTGES, PTGES2, or PTGES3, convert PGH2 to PGE2 (subsequent products of this pathway include PGA2 and PGB2 (see Prostanoid#Biosynthesis); b) PGF synthase which converts PGH2 to PGF2α; c) Prostaglandin D2 ...

*Lipid

Ceramides (N-acyl-sphingoid bases) are a major subclass of sphingoid base derivatives with an amide-linked fatty acid. The ... Hinkovska-Galcheva V, VanWay SM, Shanley TP, Kunkel RG (November 2008). "The role of sphingosine-1-phosphate and ceramide-1- ... The major phosphosphingolipids of mammals are sphingomyelins (ceramide phosphocholines),[40] whereas insects contain mainly ... a sphingolipid derived from ceramide that is a potent messenger molecule involved in regulating calcium mobilization,[74] cell ...

*Vitamin B6

Dihydroceramide is then further desaturated to form ceramide. In addition, the breakdown of sphingolipids is also dependent on ... the synthesis of ceramide requires PLP. In this reaction, serine is decarboxylated and combined with palmitoyl-CoA to form ...

*Cathepsin D

"Cathepsin D targeted by acid sphingomyelinase-derived ceramide". The EMBO Journal. 18 (19): 5252-63. doi:10.1093/emboj/18.19. ...

*어린선 - 위키백과, 우리 모두의 백과사전

ceramide synthase 3 어린선을 동반한 유전병. 병명 OMIM 유전자 단백질 CHILD Syndrome 308050 NSHDL NAD(P) dependent steroid dehydrogenase-like ...

*Dermatitis

It is unclear if moisturizers that contain ceramides are more or less effective than others. Products that contain dyes, ... Jungersted, JM; Agner, T (Aug 2013). "Eczema and ceramides: an update". Contact dermatitis. 69 (2): 65-71. doi:10.1111/cod. ...

*CERS6 (gene)

"Antiapoptotic roles of ceramide-synthase-6-generated C16-ceramide via selective regulation of the ATF6/CHOP arm of ER-stress- ... Ceramide synthase 6 is a protein that in humans is encoded by the CERS6 gene. GRCh38: Ensembl release 89: ENSG00000172292 - ... Ceramide synthase 6". Retrieved 2016-04-11. Senkal CE, Ponnusamy S, Bielawski J, Hannun YA, Ogretmen B (2010). " ...

*Galactosylgalactosylglucosylceramidase

Other names in common use include trihexosyl ceramide galactosidase, ceramide trihexosidase, ceramidetrihexoside alpha- ... Brady RO, Gal AE, Bradley RM, Martensson E (1967). "The metabolism of ceramide trihexosides. I. Purification and properties of ...

*Fumonisin B1

... but also as a substrate for ceramide synthase; aminopentol is acylated by ceramide synthase to form N-palmitoyl-AP1. This ... Inhibition of ceramide synthase is not responsible for this effect. The main factors could be DNA fragmentation and caspase-3 ... The inhibition of ceramide synthase by FB1 is thought to be reversible, since the binding is formed by noncovalent interactions ... N-palmitoyl-AP1 is an even more potent inhibitor of ceramide synthase and may therefore play a role in the toxicity of ...

*Sphinganine C4-monooxygenase

Haak, D.; Gable, K.; Beeler, T.; Dunn, T. (1997). "Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p ...

*Biosynthesis

These ceramides are synthesized from the acylation of sphingosine. The biosynthetic pathway for sphingosine is found below: As ... Sphingolipids are formed from ceramides that consist of a fatty acid chain attached to the amino group of a sphingosine ...

*Pleckstrin homology domain

Ceramide kinase, a lipid kinase that phosphorylates ceramides to ceramide-1-phosphate. Spectrin/pleckstrin-like InterPro: ... Sugiura, M.; Kono, K.; Liu, H.; Shimizugawa, T.; Minekura, H.; Spiegel, S.; Kohama, T. (2002). "Ceramide Kinase, a Novel Lipid ...

*Sphingolipid

Ceramide may subsequently have several fates. It may be phosphorylated by ceramide kinase to form ceramide-1-phosphate. ... Glycosphingolipids are ceramides with one or more sugar residues joined in a β-glycosidic linkage at the 1-hydroxyl position ( ... Ceramides, as a general class, are N-acylated sphingoid bases lacking additional head groups. Dihydroceramide is produced by N- ... Ceramide-based lipids self-aggregate in cell membranes and form separate phases less fluid than the bulk phospholipids. These ...

*COL4A3BP

Ceramide transferase protein (or CERT) is responsible for the transfer of ceramide from the endoplasmic reticulum (ER) to the ... Hanada K (2010). "Intracellular trafficking of ceramide by ceramide transfer protein". Proceedings of the Japan Academy, Series ... The transport of ceramide by CERT requires ATP. CERT - when expressed in mammalian cells - has been found to receive a lot of ... This suggests that Ceramide is still transformed to Glycosylceramide at Golgi. Moreover, it has been shown that it does not ...

*Cerevisterol

Yaoita Y, Satoh Y, Kikuchi M (2007). "A new ceramide from Ramaria botrytis (Pers.) Ricken". Journal of Natural Medicine. 61 (2 ... "A new ceramide from Suillus luteus and its cytotoxic activity against human melanoma cells". Chemistry and Biodiversity. 5 (1 ...

*UGCG

Ceramide glucosyltransferase is an enzyme that in humans is encoded by the UGCG gene. Glycosphingolipids (GSLs) are a group of ... 2001). "Role of ceramide in mediating the inhibition of telomerase activity in A549 human lung adenocarcinoma cells". J. Biol. ... 2005). "P-glycoprotein is implicated in the inhibition of ceramide-induced apoptosis in TF-1 acute myeloid leukemia cells by ... UDP-glucose ceramide glucosyltransferase catalyzes the first glycosylation step in glycosphingolipid biosynthesis. The product ...

*ASAH1

2005). "p53-Independent ceramide formation in human glioma cells during gamma-radiation-induced apoptosis". Cell Death Differ. ... Perry DK, Hannun YA (1999). "The role of ceramide in cell signaling". Biochim. Biophys. Acta. 1436 (1-2): 233-43. doi:10.1016/ ... The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene ...
TY - JOUR. T1 - A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. AU - Nickels, Joseph T.. AU - Broach, James R.. PY - 1996/2/15. Y1 - 1996/2/15. N2 - Certain mammalian growth modulators, such as tumor necrosis factor α, interleukin-1β, and γ-interferon, induce an antiproliferative response-terminal differentiation, apoptosisis, or cell cycle arrest-through a novel signal transduction pathway mediated by the lipid ceramide as a second messenger. Both a ceramide-activated protein phosphatase and a ceramide-activated protein kinase have been implicated in transmitting the signals elicited by ceramide. We have determined that ceramide addition to the yeast Saccharomyces causes a similar antiproliferative response, resulting in arrest of cells in the G1 phase of the cell cycle. We have also determined that yeast cells contain a ceramide-activated protein phosphatase composed of regulatory subunits encoded by TPD3 and CDC55 and a catalytic ...
Our results demonstrated that structural variation among ceramides leads to differences in their behavior in the membranes. The length of the long-chain base of ceramides had a larger effect on ceramide-colipid interactions compared to the effect of N-linked acyl chain length. In addition, the presence of cis-double bonds and their comparative position also caused an important effect on ceramide properties. When we studied how the lateral segregation of ceramides are influenced by the degree of unsaturation of different glycerophospholipids, we observed that these lateral segregations were affected by the overall degree of unsaturation of the sn-2 acyl chain of the PCs. We have also investigated the effect of hydrogen bonding between sphingomyelin (SM) and ceramides or cholesterol interactions. Our findings demonstrated that these interactions are markedly affected by hydrogen bonding properties. Overall, the results of this thesis showed how the gelphase forming properties of ceramides are ...
Macroautophagy is a vacuolar lysosomal catabolic pathway that is stimulated during periods of nutrient starvation to preserve cell integrity. Ceramide is a bioactive sphingolipid associated with a large range of cell processes. Here we show that short-chain ceramides (C(2)-ceramide and C(6)-ceramide) and stimulation of the de novo ceramide synthesis by tamoxifen induce the dissociation of the complex formed between the autophagy protein Beclin 1 and the anti-apoptotic protein Bcl-2. This dissociation is required for macroautophagy to be induced either in response to ceramide or to starvation. Three potential phosphorylation sites, Thr(69), Ser(70), and Ser(87), located in the non-structural N-terminal loop of Bcl-2, play major roles in the dissociation of Bcl-2 from Beclin 1. We further show that activation of c-Jun N-terminal protein kinase 1 by ceramide is required both to phosphorylate Bcl-2 and to stimulate macroautophagy. These findings reveal a new aspect of sphingolipid signaling in up-regulating
Introduction. In this study, we evaluated the anticancer activity of DM102 [(2R,3Z)-N-(1-hydroxyoctadec-3-en-2-yl)pivalamide], a novel inhibitor of the enzyme acid ceramidase (AC), a key regulator of ceramide metabolism, in combination with C6-ceramide (C6-cer) in human breast cancer cells. Ceramide, a bioactive sphingolipid and potent enhancer of apoptosis, is known to play a role in chemo- and radiation-induced cell death. AC tempers ceramide-induced apoptosis by hydrolysis, producing sphingosine and free fatty acid, thus reducing intracellular ceramide levels and providing substrate for sphingosine kinase, which converts sphingosine to sphingosine 1-phosphate. AC has emerged as a putative anticancer target following reports of upregulation in prostate cancer and in some breast tumors. In the present work, we determined whether the introduction of an AC inhibitor would enhance the apoptosis-inducing effects of C6-cer in breast cancer cells.. Methods. Cultured breast cancer cells were ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
Ceramide is a building block for complex sphingolipids in the plasma membrane, but it also plays a significant role in secondary signalling pathways regulating cell proliferation and apoptosis in response to stress. Ceramide activated protein phosphatase activity has been previously observed in association with the Sit4 protein phosphatase. Here we find that sit4Δ mutants have decreased ceramide levels and display resistance to exogenous ceramides and phytosphingosine. Mutants lacking SIT4 or KTI12 display a shift towards nonhydroxylated forms of long chain bases and sphingolipids, suggesting regulation of hydroxylase (SUR2) or ceramide synthase by Sit4p.We have identified novel subunits of the Sit4 complex and have also shown that known Sit4 regulatory subunits-SAP proteins-are not involved in the ceramide response. This is the first observation of separation of function between Sit4 and SAP proteins. We also find that the Sit4p target Elongator is not involved in the ceramide response but ...
Lipid-induced insulin resistance is associated with intracellular accumulation of inhibitory intermediates depending on the prevalent fatty acid (FA) species. In cultured myotubes, ceramide and phosphatidic acid (PA) mediate the effects of the saturated FA palmitate and the unsaturated FA linoleate, respectively. We hypothesized that myriocin (MYR), an inhibitor of de novo ceramide synthesis, would protect against glucose intolerance in saturated fat-fed mice, while lisofylline (LSF), a functional inhibitor of PA synthesis, would protect unsaturated fat-fed mice. Mice were fed diets enriched in saturated fat, n-6 polyunsaturated fat, or chow for 6 wk. Saline, LSF (25 mg/kg . d), or MYR (0.3 mg/kg . d) were administered by mini-pumps in the final 4 wk. Glucose homeostasis was examined by glucose tolerance test. Muscle ceramide and PA were analyzed by mass spectrometry. Expression of LASS isoforms (ceramide synthases) was evaluated by immunoblotting. Both saturated and polyunsaturated fat diets increased
It is known that ceramides can influence the lateral organization in biological membranes. In particular ceramides have been shown to alter the composition of cholesterol and sphingolipid enriched nanoscopic domains, by displacing cholesterol, and forming gel phase domains with sphingomyelin. Here we have investigated how the bilayer content of ceramides and their chain length influence sterol partitioning into the membranes. The effect of ceramides with saturated chains ranging from 4 to 24 carbons in length was investigated. In addition, unsaturated 18:1- and 24:1-ceramides were also examined. The sterol partitioning into bilayer membranes was studied by measuring the distribution of cholestatrienol, a fluorescent cholesterol analogue, between methyl-beta-cyclodextrin and large unilamellar vesicle with defined lipid composition. Up to 15 mol% ceramide was added to bilayers composed of DOPC:PSM:cholesterol (3:1:1), and the effect on sterol partitioning was measured. Both at 23 and 37 degrees C ...
This week we are featuring SkinMedicas TNS Ceramide Treatment Cream. This product is ideal for dry or post-procedure skin. TNS Ceramide Treatment Cream contains SkinMedicas patented TNS growth factors. These growth factors are proteins that are naturally inherent in our bodies. They play a role in both the activity of our cells and the maintenance of healthy skin. SkinMedicas patented Ceramide technology hydrates the skin and strengthens the skins natural barrier, protecting it from external elements and environmental stressors ...
Gómez-Muñoz, A., Kong, J.Y., Salh, B., Steinbrecher, U.P. (2004). Ceramide-1-phosphate blocks apoptosis through inhibition of acid sphingomyelinase in macrophages. J Lipid Res 45:99-105. [PubMed]Hinkovska Galcheva, V.T., Boxer, L.A.,Mansfield, P.J., Harsh, D.,Blackwood, A., Shayman, J.A.. (1998). The formation of ceramide-1-phosphate during neutrophil phagocytosis and its role in liposome fusion. J Biol Chem 273:50,33203. [PubMed]Gómez-Muñoz, A.,Duffy, P.A., Martin, A., OBrien, L., Byun, H.S., Bittman, R., Brindley, D.N. (1995). Short-chain ceramide-1-phosphates are novel stimulators of DNA synthesis and cell division: antagonism by cell-permeable ceramides. Mol Pharmacol 47:833-9. [PubMed ...
Gómez-Muñoz, A., Kong, J.Y., Salh, B., Steinbrecher, U.P. (2004). Ceramide-1-phosphate blocks apoptosis through inhibition of acid sphingomyelinase in macrophages. J Lipid Res 45:99-105. [PubMed]Hinkovska Galcheva, V.T., Boxer, L.A.,Mansfield, P.J., Harsh, D.,Blackwood, A., Shayman, J.A.. (1998). The formation of ceramide-1-phosphate during neutrophil phagocytosis and its role in liposome fusion. J Biol Chem 273:50,33203. [PubMed]Gómez-Muñoz, A.,Duffy, P.A., Martin, A., OBrien, L., Byun, H.S., Bittman, R., Brindley, D.N. (1995). Short-chain ceramide-1-phosphates are novel stimulators of DNA synthesis and cell division: antagonism by cell-permeable ceramides. Mol Pharmacol 47:833-9. [PubMed ...
Rice Phytoceramide is a plant-sourced phytoceramide that helps provide a plumper, smoother appearance to the skin . Ceramides make up 50% of the lipids in the skins epidermis layer. Increased water loss and skin barrier dysfunction has been linked to decreases in the amount of ceramides in the epidermis. It is known that drier skin is more susceptible to develop lines and wrinkles. The ceramides are absorbed through the digestive process and are carried by blood vessels to the outer layer of the epidermis (stratum corneum) where reinforces the skins ability to retain water, thereby providing the skin with a fuller, smoother appearance ...
As a leader in ceramide technology, Elizabeth Arden has been researching and learning more about ceramides and their role in the skin for 27 years. Research shows that while ceramides are critical to the stratum corneum, other lipids, such as cholest
Skeletal muscles exposed to excess lipid demonstrate decreased sensitivity to insulin. For example, incubating isolated muscle strips or cultured muscle cells with FFAs (22,68-71), infusing lipid emulsions into rodents or humans (72-75), or expressing lipoprotein lipase in skeletal muscle of transgenic mice (76,77) promotes intramyocellular lipid accumulation and compromises insulin-stimulated glucose uptake. To evaluate the role of ceramides in the insulin resistance associated with lipid oversupply, scientists have investigated the lipids role in FFA-induced insulin resistance using cultured myotubes. Schmitz-Pfeiffer et al. (22) first observed that treating C2C12 myotubes with concentrations of saturated FFAs within the physiological serum range increased the intracellular pool of ceramide, while simultaneously inhibiting activation of Akt/PKB, but not PI3K. In this cell type, short-chain ceramide analogs recapitulated this pattern of effects on insulin signal transduction. The authors thus ...
Although previous reports have demonstrated that dietary glucosylceramide can decrease TEWL from human skin [24], the mechanism was unclear. Ueda et al. reported that orally administrated ceramide was distributed to the dermis after intestinal absorption, followed by transfer from the dermis to the epidermis [25]. Ishikawa et al. also showed that dietary glucosylceramide was degraded into sphingoids, which were absorbed through the intestinal epithelial cells of rats and were subsequently also found in the lymph fluid in these rats [11]. Another study showed that 4-hydroxysphinganine could activate the PPARs [26] that are the key regulators of keratinocyte differentiation [27].. Keratinocyte differentiation is closely related to internal ceramide synthesis [28]. It is unlikely that orally ingested glucosylceramide would specifically localize to the skin because the amount of ingested glucosylceramide is simply too little to enhance ceramide levels in the skin. We hypothesized that sphingoids are ...
Although ceramides (Cers) are key constituents of the epidermal permeability barrier, they also function as apoptogenic signals for UVB irradiation-induced apoptosis in epidermal keratinocytes. As epidermis is continuously exposed to UV irradiation,
In this study, we found increased sphingomyelinase activity and accumulation of long‐chain ceramides in small arteries from old sheep compared to young. Additionally, we observed an increase in total collagen content in arteries from old animals. Ceramide promotes collagen deposition in dermal fibroblasts (Sato et al. 2003) implicating this lipid in the extracellular matrix remodeling of small arteries in age.. Investigation of the morphology and mechanical properties of small arteries showed that under passive conditions the mesenteric small arteries from old sheep had increased lumen diameter and media thickness without a change in media to lumen ratio, indicative of outward hypertrophic remodeling (Mulvany 1999). In addition, there was deposition of collagen in a thickened intima layer that contained both endothelial and smooth muscle cells in arteries from old animals. However, despite increased collagen content and decreased elastin to collagen ratio there was no change in stiffness in ...
Dysregulated lipid metabolism and inflammation are linked to the development of insulin resistance in obesity, and the intracellular accumulation of the sphingolipid ceramide has been implicated in these processes. Here, we explored the role of circulating ceramide on the pathogenesis of insulin resistance. Ceramide transported in LDL is elevated in the plasma of obese patients with type 2 diabetes and correlated with insulin resistance but not with the degree of obesity. Treating cultured myotubes with LDL containing ceramide promoted ceramide accrual in cells and was accompanied by reduced insulin-stimulated glucose uptake, Akt phosphorylation, and GLUT4 translocation compared with LDL deficient in ceramide. LDL-ceramide induced a proinflammatory response in cultured macrophages via toll-like receptor-dependent and -independent mechanisms. Finally, infusing LDL-ceramide into lean mice reduced insulin-stimulated glucose uptake, and this was due to impaired insulin action specifically in ...
When it comes to your skin, ceramides help create a barrier to prevent permeability. This locks moisture into your skin, preventing it from becoming dry and irritated. Read on to learn how else your skin might benefit, who needs ceramides most, how to choose the right products, and more.
AA GOLDEN CERAMIDES is a comprehensive treatment inspired by the effects of cosmetic sonophoresis. The richness of active components, such as ceramides, 24 carat cosmetic gold, oligopeptides, and hyaluronic acid neomatrix is a truly luxurious mixture that makes your skin regain exceptional smoothness and firmness. Increased effectiveness of treatment is ensured thanks to an intelligent fibre damage tracking system (INT GC3), which releases sequences of active components depending on individual needs of the skin.
Invigorate your skin with Pure Creations Elasticity Boost Eye Cream with Ceramides. This wonderful blend of ceramides, hyaluronic acid and shea butter promotes elasticity while treating dehydrated and inflamed skin.
This ceramide-rich cream contains 2% ceramides and stabilised vitamin C, retinol and hyaluronic acid to provide intense hydration that absorbs easily and improves skin smoothness, reduces wrinkles and boosts cutaneous elasticity.
Vesicle-associated membrane proteinCassociated protein (VAP) is definitely an endoplasmic reticulum (ER)-resident integral membrane protein that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to the Golgi complex by interacting with ceramide transfer protein and oxysterol-binding protein (OSBP), respectively. As demonstrated in Number 6A, endogenous VAP-A and VAP-B were coprecipitated with FLAG-Sac1 (lane 7). FLAG-Sac1 showed a higher affinity for VAP-A than VAP-B, which is definitely consistent with more proclaimed colocalization of Sac1 with VAP-A than VAP-B at the juxtanuclear storage compartments (Number 4A), although 25-Oh yea treatment did not significantly impact their relationships (Number 6A, lane 8). Additional Emergency room integral membrane proteins, calnexin and valosin-containing protein-interacting membrane protein (VIMP), were not coprecipitated with FLAG-Sac1 (Number 6A, lanes 7 and 8), suggesting that the interaction of VAPs with FLAG-Sac1 is ...
This week we are featuring SkinMedicas TNS Ceramide Treatment Cream. This product is ideal for dry or post-procedure skin. TNS Ceramide Treatment Cream contains SkinMedicas patented TNS growth factors. These growth factors are proteins that are naturally inherent in our bodies. They play a role in both the activity of our cells and the maintenance of healthy skin. SkinMedicas patented Ceramide technology hydrates the skin and strengthens the skins natural barrier, protecting it from external elements and environmental stressors ...
The positive results from the Single Dose Tolerance study allows us to proceed to the next stage of the Phase I clinical development with a Multiple Dose Safety and Tolerance study. Given the current lack of treatment options for NAFLD and NASH, CER-209 has the potential to play an important role in treating hepatic steatosis and atherosclerosis. CER-209s major asset in NAFLD and NASH treatment lies in its ability to promote HDL recognition and lipid elimination by the liver, through the activation of natural metabolic pathways mediated by the P2Y13 receptor. In addition, the studys confirmation that the pharmacokinetics of CER-209 permit once daily oral dosing is excellent news for patient treatment", said Dr. Jean-Louis Dasseux, CEO of Cerenis ...
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Ethical Permission. This study was approved by the Ethics committee at Karolinska Institutet (Etikkommitté syd).. Reagents. SR141716A and SR144528 were kind gifts from Sanofi-Aventis (Montpellier, France). R(+)-Methanandamide was purchased from Tocris Cookson Inc. (Bristol, UK). Win 55,212-2 mesylate, Fumonisin B1, l-CS, and z-VAD-FMK were purchased from Sigma Aldrich (St Louis, MO). SB202190 and SB203580 were purchased from Calbiochem Inc. (San Diego, CA), and 3,3′-dihexyloxacarbocyanine iodide was purchased from Invitrogen (Carlsbad, CA). R(+)-MA was dissolved to 10 mM in EtOH and the other reagents were dissolved to 10 mM in dimethyl sulfoxide. Further dilutions in culture medium were made on the day of the experiment.. Tumor Tissue and Controls. Tumor tissue from MCL was obtained from cryopreserved patient samples with more than 90% purity of tumor B cells. Three samples (L718, L1547, and L1676) were collected from tonsil, lymph node, and peripheral blood, respectively, of three MCL ...
Supplementary MaterialsVideo S1. Apical ECM/BM effects modeled as viscous resistances and exterior viscous level of resistance coefficient put on both areas at 8,000?Pa s m?1. Range bar is normally 20?m. Simulation period depicted over the structures. mmc3.mp4 (2.0M) GUID:?63F04E92-06A0-488F-8AD5-FA7674E03304 Video S3. Appropriate Flip Morphology Emerges with Planar Differential Development Prices and an 1187594-09-7 Explicit Description …Read More. ...
My current research activity (x35) is as follows: Invited Talks and Posters (22) American Diabetes Association (ADA) Annual Meeting, McCormick Place Convention Center (West Building), Chicago, IL; Potential for Alterations in Islet Beta-Cell Sphingolipid/Ceramide Axis Contributing to ER Stress Models of Diabetes, 21-25th June 2013 Southeastern Regional Lipid Conference (SERLC), Cashiers, North Carolina, 7-9th November…
First of all I would like to start with the appearance of the product. The jar looks awesome! White colored tub packaging looks very minimalist and futuristic. With the product you also get a little plastic spatula which helps you keep your product uncontaminated for a little longer.. Texture of a moisturizer is white and gel-ish. It has a typical non-scented chemical smell which I like. I apply the gel after I washed, toned and nourished my skin with serums and essences. When I patted it into my skin it absorbed really quickly (5-8 min).. The other day I put it on way more and it needed longer time to absorb, and that day I skipped serums and essences, because it claims that it can provide five basic skincare elements: Lotion, Beauty Serum, Emulsion, Cream, and Mask.. ...
RatzillaCosme is a complete and comprehensive guide since 2009 to Japanese cosmetics and beauty products. Everything on this site (including ingredients) is outlined by me based on official Japanese press releases ...
Dermex-P is quickly becoming one of the most popular phytoceramides capsules around, but how does Dermex-P work? What Do the Dermex-P pill reviews really say? What Is Dermex-P? Dermex-P is a superior form of ceramides, a rice derivative lipid that… Continue Reading → The post Dermex-P Pill Reviews appeared first on Miracle Phytoceramides Reviews ...
Ceramides are a family of waxy lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high concentrations within the cell membrane of cells, since they are component lipids that make up sphingomyelin, one of the major lipids in the lipid bilayer. Contrary to previous assumptions that ceramides and other sphingolipids found in cell membrane were purely supporting structural elements, ceramide can participate in a variety of cellular signaling: examples include regulating differentiation, proliferation, and programmed cell death (PCD) of cells. The word ceramide comes from the Latin cera (wax) and amide. Ceramide is a component of vernix caseosa, the waxy or cheese-like white substance found coating the skin of newborn human infants. There are three major pathways of ceramide generation. The sphingomyelinase pathway uses an enzyme to break down sphingomyelin in the cell membrane and release ceramide. The de novo pathway creates ceramide from less complex ...
Background: Among the 5 (L1-L5) chromatographically resolved subfractions of human LDL, L5 is the most electronegative and atherogenic. L5s atherogenicity may be attributed in part to its high ceramide content. Because ceramides can be converted from sphingomyelins by sphingomyelinase (SMase), we examined whether L5 has SMase-like properties.. Methods and Results: The total lipid extract of L5 was as capable as L5 in inducing arterial endothelial cell (EC) apoptosis. LC/MSE analysis confirmed that L5 had a significantly greater ceramide content in the lipid extract than L1 (n=4; P,0.01). On Amplex Red SMase assays, L5 but not L1 exhibited a strong SMase-like activity (n=12). ECs treated with L5 increased intracellular ceramide concentrations; the associated cytotoxicity was significantly attenuated by SMase inhibitor desipramine. To identify the putative region of LDL with SMase activities, we compared human apolipoprotein B100 (apoB100) with Bacillus cereus, Leptospira interrogans, and ...
Ceramide synthase 5 (CerS5) is the enzyme encoded in humans by the CERS5 gene. CerS5 robustly synthesizes C16-ceramide, which is often considered to be an important pro-apoptotic ceramide. De novo ceramide synthesis is an essential trigger for Bax activation in hypoxia/reoxygenation. Following hypoxia/reoxygenation, CerS5 expression is elevated. Upon knocking down acid sphingomyelinase and CerS5 in NTERA-2 cells, Bax localization to mitochondria was reduced, indicating the importance of CerS5 activity in the apoptosis pathway. CerS5 (TRH4) mRNA is found in all tissues and is strongly expressed in muscle and brain. CerS5 is the major ceramide synthase detected in lung epithelia. Knock-down research in respiratory epithelium using CerS5 siRNA or fumonisin B1 reduced total CerS activity by 45% or 78%, respectively, indicating that CerS5 indeed contributes significantly to ceramide synthesis in lung. In the brain, CerS5 mRNA is detected in most cells within the gray and white matter tissues. CerS5 ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.. ...
Ceramides are the main polar lipids of the SC intercellular lipids; they play an important role in skin barrier function and account for half of the SC lipids by weight. Nine subclasses of ceramides have been described in human SC; these differ from each other by the sphingoid base groups (sphingosine, phytoshpingosine, 6-hydroxysphingosine) and hydrocarbon chain length. Ceramide content and changes of composition are involved in agingand skin diseases such as atopic dermatitis (AD),5-7 psoriasis, contact dermatitis (CD) and irritant dermatitis.
AIMS/HYPOTHESIS: An accumulation of ceramides has been implicated in the generation of insulin resistance in skeletal muscle upon an oversupply of fatty acid. Different ceramide species are generated through the actions of ceramide synthases (CerSs), which incorporate specific acyl side chains. We tested whether particular CerS isoforms promoted insulin resistance through the generation of more inhibitory ceramide species, thus representing potential targets for intervention. METHODS: CerS isoforms CerS1, CerS2, CerS4, CerS5 and CerS6 were overexpressed in L6 myotubes using adenovirus, and cells were treated with palmitate and stimulated with insulin. Alternatively, CerS isoforms were knocked down using siRNAs. Sphingolipids were examined by mass spectrometry and tracer incorporation. Phosphorylation of IRS1 and Akt was measured by immunoblotting, while glucose disposal was assessed by measuring GLUT4 translocation and the incorporation of [(14)C]glucose into glycogen. RESULTS: Palmitate treatment
Sphingolipids such as ceramide and S1P have been recognized as bioactive lipids in cell death and proliferation/survival. In this review we overviewed role for sphingolipids in the induction of cell death in several types of hematological malignancies such as leukemia, malignant lymphoma and multiple myeloma. The main backbone molecule of sphingolipid network is ceramide. Ceramide is synthesized from serine and fatty acids with different kinds of carbon chains by de novo ceramide pathway in endoplasmic reticulum, and transferred to the Golgi apparatus by CERT for synthesis of SM by SMS while ceramide transfer protein for glucosylceramide to Golgi apparatus remains unknown. Sphingosine generated by CDase is phosphorylated and turns to S1P by SK1 or SK2. The complex sphingolipids such as glucosylceramide and GM3 are transferred to the micro-organelles to exert their functions and again returned to ceramide by salvage pathway. Likely, the catabolized sphingolipids such as sphingosine and ...
We investigated sphingolipid metabolism in human adipose tissue to identify pathways underlying increased ceramide concentrations in inflamed adipose tissue [3]. Our data suggest that hydrolysis of sphingomyelin to ceramide by sphingomyelinases could explain, at least partly, this increase. Gene expression levels of SMPD3 correlate significantly with concentrations of various ceramides and sphingomyelins in subcutaneous adipose tissue, and are higher in the relatively more inflamed intra-abdominal compared to the subcutaneous depot in both obese and non-obese subjects. Sphingomyelinases are expressed by both adipocytes and macrophages in adipose tissue, but their expression is strongest in and around blood vessels. Our findings implicate a role for sphingomyelinase-mediated generation of ceramide in adipose tissue inflammation.. When comparing inflamed ceramide-rich and relatively less inflamed ceramide-poor subcutaneous adipose tissue of obese women, we report here for the first time that there ...
Cystic fibrosis, a genetic disease associated with frequent lung infections and a shortened life span, is caused by a defect in the CFTR gene, which encodes a membrane transporter. Although it is not clear exactly how defective CFTR links to the symptoms, the mutant protein is known to increase the pH in intracellular organelles. On the basis of results obtained from patients cells and from mice carrying mutated Cftr (which produces a cystic fibrosis-like disease), Teichgräber et al. suggest that this rise in pH increases susceptibility to lung infection by altering levels of ceramide, a membrane constituent that can also trigger cell death. The higher pH inhibits the enzyme that breaks down ceramide, and the resulting excess of ceramide increases vulnerability to lung infection. Blocking the biosynthesis of ceramide via acid sphingomyelinase normalizes ceramide levels and, most tellingly, renders the Cftr-deficient mice resistant to lung infections. This block can be achieved with ...
Increased availability of free fatty acids impairs glucose disposal in young healthy humans. Patients with type 2 diabetes have reduced whole body glucose disposal, increased ectopic lipid deposition in skeletal muscle and the liver and impaired mitochondrial function. Recent studies suggest that lipid metabolites such as diacylglycerol (DAG), ceramides and long-chain acyl-coA represent the active mediators inducing insulin resistance. Possible targets are DAG-sensitive Proteinkinase C (PKC θ, PKC ε) which inhibit the insulin signaling cascade and ceramides which interfere with the insulin signaling cascade at Proteinkinase B/AKT. Prior studies raised controvesial evidence, thus, it is yet unclear, whether DAG or ceramides are the primary agents inducig lipid-induced insulin resistance. Therefore, the current study aims to explore the time course of the appearance of intramyocellular lipid compunds during lipid infusion in parallel assessing markers of impaired insulin action ...
Current investigations are giving new insight about the roles of sphingolipids in cellular regulation of growth suppression, apoptosis and signal transduction. Among the sphingolipids, ceramide has emerged as an important second signal mediator, having multiple cellular and biochemical targets. In particular, ceramide act as a potent cell death inducer and a specific cell growth suppressor. Recently, it has been thought that generation of ceramide is a universal feature of apoptosis [6, 44]. Presently, a few drugs are available in combinatorial chemotherapy, which could generate ceramide. Therefore, to find pharmacological modulators that induce ceramide production in malignant cells by mounting a physiological response and induce senescence is on prime importance.. In the present study, we demonstrated rapid accumulation of endogenous ceramide in WithaD treated MOLT-4 cells. In fact, 61.55% cells were ceramide positive just in 30 mins. WithaD showed almost four fold increase in ceramide level ...
Ceramide, a key intermediate in sphingolipid metabolism, is synthesized by acylation of sphinganine followed by dehydrogenation of dihydroceramide to ceramide. Using radioactive sphinganine, we have examined the site and topology of dihydroceramide synthesis in well-characterized subcellular fractions from rat liver. [4,5-3H]Sphinganine was introduced as a complex with BSA and was metabolized to [4,5-3H]dihydroceramide upon incubation of rat liver homogenates or microsomes with fatty acyl CoA. Conditions were established in a detergent-free system in which dihydroceramide synthesis was not limited by either substrate availability or by amounts of microsomal protein or reaction time. The distribution of dihydroceramide synthesis was found to exactly parallel that of an endoplasmic reticulum (ER) marker upon subfractionation of microsomes, and no endogenous activity was detected in either purified Golgi apparatus or plasma membrane fractions. Limited protease digestion demonstrated that ...
Phytoceramides were recently featured on the Dr. Oz television show, and since that time, they have been incredibly popular. Phytoceramides are used to help fight the aging process, reduce wrinkles, and prevent fine lines from occurring. What is interesting about Phytoceramides Walgreens is that they are completely natural ingredients and that they really do work.. Dr. Oz Phytoceramides are considered to be the most effective of these anti-aging pills, as they help to reintroduce plant derived ceramides into the system. Ceramides are the natural protein molecules that help to keep the moisture barrier within the skin intact so that the skins health can be improved.. ...
Numerous prior studies suggest a close relationship between the regulation of ceramide levels through GCS and the induction of apoptosis. First, the GCS inhibitor 1-phenyl-2-decanoylamino-3- morpholino-1-propanol enhanced apoptosis in the presence of C6-ceramide in CHP-100 human neuroepithelioma cells (30) . Second, increased GCS protein levels and activity are evident in DOX-resistant human breast cancer cells (MCF-7-AdrR; ref. 31 ), and inhibition of GCS (using antisense) reversed resistance to DOX in these cells (32) . Third, overexpression of GCS conferred resistance to DOX in MCF-7 or HL-60 cells (19 , 33) . Finally, overexpression of GCS induced resistance to ceramide-induced apoptosis in human hepatoma Huh 7 cells (20) and attenuated ceramide-induced growth inhibition in human keratinocytes (21) . Moreover, our prior studies showed that the relative GCS activity is approximately 10- to 100-fold higher than that of other ceramide-metabolizing enzymes, such as sphingomyelin synthase and ...
Explore the benefits of ceramides in skincare. Ceramides maintain skin-hydration & have been suggested to have soothing properties that can alleviate dry skin.
From this experience, I realised the importance of using the right product which is suited for sensitive skins. It was something I had overlooked and thinking back, if only I had known it earlier…. I wouldnt have to go through those episodes. Sensitive skin is actually caused by a lack of ceramide, which is an essential ingredient in the skins as it helps to replenish moisture between the skin cells. Ceramides are naturally found within our skins, however sometimes our body dont produce enough of it, leading us to have ceramide-deficient skin. When this happens, our skin is likely to be aggravated by external irritation, causing problems like dryness, chapping, redness and itch ...
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear ...
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Ceramides play a central role in preserving healthy-looking skin. They are an important lipid component of the skins membrane‚ where they provide protection from the elements and lock in moisture. In fact‚ they make up 35%-40% of the binding matrix that maintains moisture balance and protects the skins surface. The problem is that the bodys production of ceramides declines with age. The ceramides that young skin naturally produces to retain its supple appearance are identical to those present in wheat‚ which is why wheat-derived oils have been used topically for centuries as a natural moisturizer. However‚ you cant get enough ceramides from topically-applied wheat germ oil to have a long-term impact on your skins appearance‚ and they dont appear in sufficient concentration in your diet. Thats where a supplement like Life Extensions Skin Restoring Ceramides with Lipowheat can help. This product brings together skin-nourishing oils in an oral formula with Lipowheat™‚ a ...
Acid sphingomyelinase-induced ceramide release has been shown by many studies to induce apoptosis in response to various stimuli. However, the mechanisms of acid sphingomyelinase/ceramide-mediated dea
This was a laboratory study, primarily conducted in mice, though there was an aspect of human-cell analysis. The researchers were trying to get a better understanding about the biological processes that take place in the lungs when an organism has cystic fibrosis. Cystic fibrosis is the most common inherited life-threatening disease that occurs among Caucasian people in the UK. In an experiment conducted in several different parts, the researchers used strains of mice with a mutation that gave them cystic fibrosis. They were particularly interested in the role of a particular lipid called ceramide. In the first part of the experiment, the researchers compared the levels of ceramide in the lungs of the mutant mice with the levels in healthy mice. They did further experiments to identify in exactly which cells ceramide was accumulating. The researchers also looked at whether the ceramide concentration had any effect on pH (i.e. acidity) levels in the respiratory cells. The researchers were testing ...
NIACINAMIDE SerumReduces water loss by 24% in 4 weeks, boosts fatty acid and ceramides by 67% and 34% respectively; stimulates new fibroblasts by 20%
NIACINAMIDE Serum (anti-aging serum)Reduces water loss by 24% in 4 weeks, boosts fatty acid and ceramides by 67% and 34% respectively; stimulates new
Collagen30 Tablets with Ceramides, 120 units. Discover the variety of products, detailed information, prices and availability in stores or online.
OGT2378 is a GCS inhibitor (Glucosyl Ceramide Synthase Inhibitor) with potential anticancer activity. In murine models with melanoma, OGT2378 decreased tumor size, and showed no cytotoxic and apoptotic effects on MEB-4 melanomacells. Additionally, it has been reported that OGT2378 is biologically active, and a well tolerated agent in in vivo system.
In this book many aspects of ceramide and sphingolipid biology are addressed. The focus of this book is the metabolism of ceramide in normal and diseased states and the biophysical and biochemical mechanisms governing the bioactivity of these molecules.
The primary objective of this study is to obtain data regarding the safety of olipudase alfa in patients with acid sphingomyelinase deficiency (ASMD) who are exposed to long term treatment with olipudase alfa.. The secondary objectives of this study are to obtain data regarding the efficacy of olipudase alfa and to characterize olipudase alfa pharmacodynamics (PD) and pharmacokinetics (PK) following long-term administration. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
First luxurious anti-wrinkle care from AA brand with a division into skin types. The ageing process of the skin does not depend entirely on age, but also on many other factors: genes, lifestyle, diet, external environmental factors and solar radiation.
Posted on 08/07/2013 7:35:02 PM PDT by CutePuppy. The scientists discovered that the ceramide-1 phosphate transport protein (CPTP) regulates levels of biologically active lipids, which are molecules such as fatty acids that often play a role in cell signaling. They found that CPTPs main function is to transport ceramide-1-phosphate (C1P), a lipid that helps regulate cell growth, survival, migration and inflammation. Specifically, C1P increases the production of pro-inflammatory eicosanoids - powerful signaling molecules that contribute to chronic inflammation in diseases such as cancer, asthma, atherosclerosis and thrombosis - and the discovery of CPTP sheds a light on the cellular mechanisms that contribute to these diseases. We may have identified the newest target for treating cancer. Because of the important role this protein plays in a number of cellular functions, it could also have large implications for a variety of diseases like cancer that are caused by inflammation, The team was ...
We have investigated whether the increases in ceramide levels that occur during apoptosis in SKW 6.4 cells induced by anti-Fas antibody depend on the activation of caspases. Using cells prelabelled to equilibrium with ...
E & C, lycopene-rich tomato extract and ceramides.Suitable for all skin types, even with extremely dry skin caused by neuro-dermatitis. Skin feels noticeably firmer and smoother. ...
The signalling mechanisms responsible for the hydrolysis of sphingomyelin mediated by 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and interferon γ (IFN-γ) in HL-60 cells were investigated. IFN-γ was found to increase selectively the activity of cytosolic, Mg2+-independent, neutral sphingomyelinase. The treatment of HL-60 cells with the combination of 1,25(OH)2D3 and IFN-γ had an additive effect on sphingomyelin hydrolysis, ceramide release and the activity of cytosolic, Mg2+-independent, neutral sphingomyelinase. The pretreatment of HL-60 cells with staurosporine, chelerythrine chloride and bisindolylmaleimide abolished the activity of sphingomyelinase in response to 1,25(OH)2D3 and IFN-γ. Calphostin C, which acts on the regulatory site of protein kinase C (PKC), and Gö 6976, a selective inhibitor of Ca2+-dependent PKC isoforms, inhibited the effect of 1,25(OH)2D3 but had no effect on the IFN-γ-mediated increase in activity of sphingomyelinase. Isoform-specific antibodies were used to deplete ...
The effects of salicylates on insulin resistance. There is significant overlap between the intracellular events induced by TGs (or FFAs) and TNF regarding the mechanisms of insulin resistance. Both stimuli activate IKK-β and decrease insulin-induced tyrosine phosphorylation of IRS-1; both increase intracellular ceramide concentrations, which leads to inhibition of Akt/protein kinase B activation and inhibition of GLUT-4 translocation. These effects induce a state of insulin resistance. The effects of salicylate compounds on these pathways may be divergent. That is, they may improve insulin resistance by blocking the activation of IKK-β (top), or they may worsen insulin resistance by inhibiting PG synthesis and thus potentiating TNF release (bottom). In addition, inhibition of PG will decrease synthesis of leptin, which is known to improve insulin sensitivity by stimulating IRS-1-associated PI 3-kinase activity. The beneficial effect of leptin on insulin action is thus decreased (bottom ...
BACKGROUND: Oxalate exposure produces oxidant stress in renal epithelial cells leading to death of some cells and adaptation of others. The pathways involved in these diverse actions remain unclear, but appear to involve activation of phospholipase A2 (PLA2) and redistribution of membrane phospholipids. The present studies examined the possibility that oxalate actions may also involve increased accumulation of ceramide, a lipid-signaling molecule implicated in a variety of pathways, including those leading to apoptotic cell death. METHODS: Ceramide accumulation was examined in renal epithelial cells from pig kidney (LLC-PK1 cells) and from dog kidney [Madin-Darby canine kidney (MDCK cells)] using the diacylglycerol kinase assay. Sphingomyelin degradation was assessed by monitoring the disappearance of 3H-sphingomyelin from cells that had been prelabeled with [3H]-choline. The effects of oxalate were compared with those of other oxidants (peroxide, xanthine/xanthine oxidase), other organic acids (formate
0251] Citation of a reference herein shall not be construed as an admission that such is prior art relevant to patentability of the present invention. Specifically incorporated herein by reference in their entireties are the following publications: [0252] Amer S, Rawal N, Gustafsson L L. Clinical experience of long-term treatment with epidural and intrathecal opioids--a nationwide survey. Acta Anaesthesiol Scand 1988; 32:253-259 [0253] Bartolome J, Castillo I and Carreno V, Ribozymes as antiviral agents, Minerva Med. 2004 95:11-24 [0254] Baum, Solid-phase synthesis of benzodiazepines, C&EN, Jan. 18, 1993, page 33. [0255] Bektas M, Jolly P S, Milstien S and Spiegel S., A specific ceramide kinase assay to measure cellular levels of ceramide, Analytical Biochemistry 2003 320:259-265. [0256] Bethea J R, Castro M, Keane R W, Lee T T, Dietrich W D and Yezierski R P, Traumatic spinal cord injury induces nuclear factor-kappaB activation, J Neurosci 1998 18:3251-3260. [0257] Blazquez C, Galve-Roperh I ...
May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.
Background: Ceramide (Cer) participates in neuronal and oligodendroglial cell death and neuroinflammation. We have previously shown that Cer levels increase in the cerebrospinal fluid (CSF)of patients with subarachnoid hemorrhage (SAH). The goal of this study is to further characterize the origin of Cer after SAH.. Methods: Cer and dihydroceramide (DHC) levels as well as the activity of Acid (ASMase) and Neutral sphingomyelinase (NSMase) were measured in the CSF of SAH patients and controls. The de novo synthesis of Cer was estimated by correlating Cer with DHC levels using Spearman correlation. The activity of intracellular enzymes that regulate Cer homeostasis including ASMase, NSMase, sphingomyelinase synthase (SMS) and glucosylceramide synthase (GCS) was investigated using the rat model of SAH. Brain homogenates were used for in vitro enzyme activity measurement. The results were compared using non-parametric analysis.. Results: A total of 26 SAH patients and 8 controls were included in the ...
TABLE-US-00001 NAME OF RAW MATERIAL WEIGHT % (MANUFACTURER) INCI 1 2 3 4 5 6 7 8 9 10 Pseudoceramides Pseudoceramide 176 N-(1-dodecanoyl)-4- 0.1 0.2 0.1 hydroxy-L-proline 1- hexadecyl ester Pseudoceramide 391 N-(1-hexadecanoyl)-4- 0.2 0.1 0.1 0.1 0.2 0.5 hydroxy-L-proline 1- hexadecyl ester Ceramide PC104 (Pacific hydroxypropyl- 0.1 Corporation) bispalmitamide MEA Ceramide SL (Sino Lion) hydroxyethyl-palmityl- 0.1 oxyhydroxypropyl- palmitamide Aqua-Ceramide (Kao) cetyloxypropyl-glyceryl- 0.1 0.1 methoxypropyl- myristamide Ceramides Ceramide 2 (Sederma) ceramide 2 0.1 Ceramide III (Cosmoferm) ceramide 3 0.1 Bisabolol (Alpha-)Bisabolol, natural bisabolol 0.2 0.3 0.2 0.5 0.1 (Symrise) Dragosantol (Symrise) bisabolol 0.3 0.4 0.2 0.3 0.3 Fatty acids Linoleic Acid linoleic acid 0.1 0.2 Palmitic Acid palmitic acid 0.1 0.1 0.2 Stearic Acid stearic acid 0.1 Sterols Cholesterol (Croda) cholesterol 0.1 0.5 Tamasterol (Tama Biochemicals) phytosterol 0.2 Other ingredients Abil 350 (Degussa- dimethicone 0.5 ...
13 products from 5 suppliers. Compare and order Sphingomyelin phosphodiesterase 2, Neutral Membrane (Neutral Sphingomyelinase) ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended products for the most popular species. Our scientists will help you find the right ELISA kit for your needs.
BioAssay record AID 147531 submitted by ChEMBL: Inhibitory activity against schyphostatin of neutral sphingomyelinase (N-SMase) from bovine brain microsome.
In this study, we show a novel and essential role of SMS2 in modulating NFκB activation. This is based on the following observations: in both SMS2 KO mouse macrophages and SMS2 knockdown HEK293 cells, (1) SMS activity, de novo SM synthesis, cellular and plasma membrane SM levels were significantly decreased, (2) ligand-induced NFκB activation, including IκBα degradation and NFκB nuclear translocation, as well as transcriptional activation, were significantly attenuated, and (3) LPS-induced membrane recruitment of TLR4-MD2 complex and TNF-α-induced raft association of TNFR1 were impaired in SMS2 KO macrophages and SMS2 siRNA-treated HEK293 cells, respectively.. SMS2 makes an important contribution to the de novo SM biosynthesis and total cellular SM levels. Based on their relative proximity to the site of ceramide biosynthesis, it has been suggested that SMS1 might be involved in the de novo SM biosynthesis whereas SMS2 is involved in the remodeling of plasma membrane structure.28 However, ...
BioAssay record AID 204026 submitted by ChEMBL: Inhibitory potency of compound against neutral (N-) SMase activity in bovine brain microsomes(Ms) at concentration of 400 uM.
Looking for online definition of ceramide saccharide in the Medical Dictionary? ceramide saccharide explanation free. What is ceramide saccharide? Meaning of ceramide saccharide medical term. What does ceramide saccharide mean?
The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues varied in the acyl chain length and the position of the functional group for click chemistry. Fluorescence microscopy studies including anisotropy and quenching experiments showed signifi
Involved in the production of sphingolipid metabolites. Active on sphingosine, phytosphingosine (PHS, 4-hydroxysphinganine), D-erythro-dihydrosphingosine, D-erythro-sphingosine and trans-4, trans-8-sphingadienine, an LCB found exclusively in plants, but not on N-acetyl-dihydrosphingosine (C2-dihydroceramide) and D-threo-dihydrosphingosine.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Sphingolipids are a conserved family of lipids built upon a sphingoid base backbone. Sphingolipids serve structural membrane functions, whereas their metabolism...
Sphingolipids are a family of membrane lipids with important structural roles in the regulation of the fluidity and subdomain structure of the lipid bilayer, es...
PIK-93 is a potent PI3K inhibitor. PIK93 selectively inhibits the type III PI 4-kinase beta enzyme, and small interfering RNA-mediated down-regulation of the individual PI 4-kinase enzymes, revealed that PI 4-kinase beta has a dominant role in ceramide transport between the ER and Golgi.
Oat kernel oil is a golden lipid extracted from oats with amazing moisturising and healing properties and a non-greasy finish. Its a fast-absorbing oil packed with linoleic fatty acid (omega 6), sterols, ceramides and sphingolipids, antioxidants and anti-inflammatory agents. Oat kernel oil is non-comedogenic and can even reduce the appearance… ...
Hyaluronic acid helps retain moisture in the skin and keeps it looking plump for a more youthful-looking appearance. But it s about more than just hydration; the skin also needs lipids, such as cholesterol, free fatty acids and ceramides, to keep the skin moisture barrier healthy. Your skin will be on an all-time high with an Epionce hyaluronic acid plus essential lipid combo ...
I2PP2A/SET is overexpressed and C18-ceramide is decreased in primary lung tumour tissuesTen paired samples of fresh frozen lung adenocarcinoma tumours (T) with
Your hands seem to be dehydrated and prone to sensitivity due to the climate and the exposure to ingredients that may be high in surfactant level. Washing with soap and water, even though mildly irritating for most, can be more irritating when done frequently. If you cannot choose a cleanser with lower surfactant levels, then youll want to protect yourself from future damage. If you also use exfoliants, use gloves and a brush if possible. Less is better in this instance. Also, replenish your natural lipids with a product containing lipids such as Ceramides, Sterols and fatty acids.. ...
best ceramide skincare products,Perfect for problematic and photo-damaged skin, Retinol 1.0 contains 1% pure retinol, the highest concentration currently available ... skin and follow with other skin care products. As this retinol cream ...
Nourish and even complexion with Tint Du Soleil Broad Spectrum SPF 30 UV Protective Foundation by Colorescience. Multi-vitamin complex, peptides, ceramides and milk lipids improve the appearance of skin tone while the creamy, hydrating whipped formula feels like bare skin at its best.
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Researchers found that MS patients with high body mass indexes (BMIs) had higher ceramide levels and more circulating monocytes than were evident in healthy individuals with the same BMIs.
Dermex-P is quickly becoming one of the most popular phytoceramides capsules around, but how does Dermex-P work? What Do the Dermex-P pill reviews really say? What Is Dermex-P? Dermex-P is a superior form of ceramides, a rice derivative lipid that… Continue Reading → The post Dermex-P Pill Reviews appeared first on Pure Garcinia Cambogia Reviews ...
Dermex-P is quickly becoming one of the most popular phytoceramides capsules around, but how does Dermex-P work? What Do the Dermex-P pill reviews really say? What Is Dermex-P? Dermex-P is a superior form of ceramides, a rice derivative lipid that… Continue Reading → The post Dermex-P Pill Reviews appeared first on Pure Garcinia Cambogia Reviews ...
Japanese Sake Skin Care Emulsion - The Japanese Sake Skin Care Emulsion from Kiku Masamune features an amino acid compound and ceramides that will moisturize your skin. After using this emulsion, your skin will look and feel hydrated, smooth, and healthily glossy. Harnessing the natural qualities of Japanese sake and its rice ferment ...
Targeted care for bruised skin - with ceramides and arnica oil. DerMend ® Moisturizing Bruise Formula helps improve the appearance of bruised skin
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The serine palmitoyl transferase inhibitor myriocin potently suppresses the development of atherosclerosis in apolipoprotein E (apoE) gene knockout (apoE-/-) mice fed a high-fat diet. This is associated with reduced plasma sphingomyelin (SM) and glycosphingolipid levels. Furthermore, oral administration of myriocin decreases plasma cholesterol and triglyceride (TG) levels. Here, we aimed to determine whether myriocin could inhibit the progression (or stimulate the regression) of established atherosclerotic lesions and to examine potential changes in hepatic and plasma lipid concentrations. Adult apoE-/- mice were fed a high-fat diet for 30 days, and lesion formation was histologically confirmed. Replicate groups of mice were then transferred to either regular chow or chow containing myriocin (0.3 mg/kg/day) and maintained for a further 60 days. Myriocin significantly inhibited the progression of established atherosclerosis when combined lesion areas (aortic sinus, arch, and celiac branch point) were
Among many mycotoxins, T-2 toxin, macrocyclic trichothecenes, fumonisin B1 (FB1) and ochratochin A (OTA) are known to have the potential to induce neurotoxicity in rodent models. T-2 toxin induces neuronal cell apoptosis in the fetal and adult brain. Macrocyclic trichothecenes bring about neuronal cell apoptosis and inflammation in the olfactory epithelium and olfactory bulb. FB1 induces neuronal degeneration in the cerebral cortex, concurrent with disruption of de novo ceramide synthesis. OTA causes acute depletion of striatal dopamine and its metabolites, accompanying evidence of neuronal cell apoptosis in the substantia nigra, striatum and hippocampus. This paper reviews the mechanisms of neurotoxicity induced by these mycotoxins especially from the viewpoint of oxidative stress-associated pathways.
Sphingolipids are bioactive molecules associated with oxidative stress, inflammation, and neurodegenerative diseases, but poorly studied in the context of age-related macular degeneration (AMD), a prevalent sight-threatening disease of the ageing retina. Here, we found higher serum levels of hexosylceramide (HexCer) d18:1/16:0 in patients with choroidal neovascularization (CNV) and geographic atrophy (GA), two manifestations of late stage AMD, and higher ceramide (Cer) d18:1/16:0 levels in GA patients. A sensitivity analysis of genetic variants known to be associated with late stage AMD showed that rs1061170 (p.Y402H) in the complement factor H (CFH) gene influences the association of Cer d18:1/16:0 with GA. To understand the possible influence of this genetic variant on ceramide levels, we established a cell-based assay to test the modulation of genes in the ceramide metabolism by factor H-like protein 1 (FHL-1), an alternative splicing variant of CFH that also harbors the 402 residue. We first ...
TY - JOUR. T1 - Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases. AU - Narita, Tomomi. AU - Naganuma, Tatsuro. AU - Sase, Yurie. AU - Kihara, Akio. PY - 2016/5/3. Y1 - 2016/5/3. N2 - Transport of dietary lipids into small-intestinal epithelial cells is pathologically and nutritionally important. However, lipid uptake remains an almost unexplored research area. Although we know that long-chain bases (LCBs), constituents of sphingolipids, can enter into cells efficiently, the molecular mechanism of LCB uptake is completely unclear. Here, we found that the yeast acyl-CoA synthetases (ACSs) Faa1 and Faa4 are redundantly involved in LCB uptake. In addition to fatty acid-activating activity, transporter activity toward long-chain fatty acids (LCFAs) has been suggested for ACSs. Both LCB and LCFA transports were largely impaired in faa1Δ faa4Δ cells. Furthermore, LCB and LCFA uptakes were mutually competitive. However, the energy dependency was different for ...
SCALCO, F.B.; GIUGLIANI, R.; TOBO, P. and COELHO, J.C.. Effect of dimethylsulfoxide on sphingomyelinase activity and cholesterol metabolism in Niemann-Pick type C fibroblasts. Braz J Med Biol Res [online]. 1999, vol.32, n.1, pp.23-28. ISSN 1414-431X. http://dx.doi.org/10.1590/S0100-879X1999000100003.. Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism. The influence of dimethylsulfoxide (DMSO) on the amount of intracellular cholesterol was measured in 8 cultures of normal fibroblasts and in 7 fibroblast cultures from NPC patients. DMSO was added to the fibroblast cultures at three different concentrations (1, 2 and 4%, v/v) and the cultures were incubated for 24 h. Sphingomyelinase activity was significantly increased in both groups of cells only when incubated with 2% DMSO (59.4 ± 9.1 and 77.0 ± 9.1 nmol h-1 mg protein-1, controls without and with 2% DMSO, respectively; 47.7 ± ...
The sphingolipid metabolites and genes (with the gene abbreviations shown in boxes, or enzyme names where gene names are ambiguous) are given for the condensation of serine and palmitoyl-CoA to form 3-ketosphinganine (3-ketoSa) by serine palmitoyltransferase, which is reduced to sphinganine (Sa), acylated to dihydroceramides, DHCer, by (DH)Cer synthases, and incorporated into more complex DH-sphingolipids (the 1-phosphate, DHCerP, sphingomyelins, DHSM, glucosylceramides, DHGlcCer, galactosylceramides, DHGalCer, lactosylceramides, DHLacCer, and sulfatides, or desaturated to Cer followed by headgroup addition. Also included are a number of the catabolic genes, e.g., sphingomyelinases, SMases, ceramidases, ASAH, sphingosine kinases, for the formation of sphinganine 1-phosphate (Sa1P) and sphingosine 1-phosphate (So1P), and phosphatases for the reverse reaction and the lyase that cleaves sphingoid base 1-phosphates to ethanolamine phosphate (EP), hexadecanal (C16:0al) and hexadecenal (C16:1al ...
Product Ingredients Water (Aqua), Sodium Ascorbyl Phosphate (vitamin C/antioxidant), Cetyl Alcohol (texture enhancer, Ascorbyl Glucoside (vitamin C/antioxidant), Neopentyl Glycol Diheptanoate (texture enhancer), Potassium Cetyl Phosphate (emulsifier), Glyceryl Stearate (emollient), PEG-100 Stearate (texture enhancer), Glycerin (skin-replenishing), PEG-12 Glyceryl Dimyristate (hydration), Bis-Diglyceryl Polyacyladipate-2 (emollient), Dimethicone (emollient), Glyceryl Ascorbate, Tetrahexyldecyl Ascorbate (vitamin C/antioxidants), Retinol (vitamin A/skin-restoring), Ceramide NP, Ceramide NS, Ceramide AP, Ceramide EOP, Ceramide EOS, Cholesterol, Caprooyl Phytosphingosine, Caprooyl Sphingosine (skin-replenishing), Squalane (emollient), Punica Granatum Fruit Extract (pomegranate/antioxidant), Pongamia Glabra Seed Oil (non-fragrant plant oil/antioxidant), Glycyrrhiza Glabra (Licorice) Root Extract (skin-soothing), Vitis Vinifera (Grape) Seed Extract (antioxidant), Tocopheryl Acetate (vitamin ...

Sphingolipid Ceramide N-Deacylase | SCDase | Sphingolipid HydrolysisSphingolipid Ceramide N-Deacylase | SCDase | Sphingolipid Hydrolysis

Sphingolipid Hydrolysis: Sphingolipid Ceramide N-Deacylase (SCDase) Sphingolipid ceramide N-deacylase (SCDase) is derived from ... Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. ...
more infohttp://www.clontech.com/US/Products/Glycobiology/Glycobiology_Enzymes_and_Reagents/SCDase

Sphingolipid Ceramide N-Deacylase | SCDase | Sphingolipid HydrolysisSphingolipid Ceramide N-Deacylase | SCDase | Sphingolipid Hydrolysis

Sphingolipid Hydrolysis: Sphingolipid Ceramide N-Deacylase (SCDase) Sphingolipid ceramide N-deacylase (SCDase) is derived from ... Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. ...
more infohttp://www.clontech.com/CA/Products/Glycobiology/Glycobiology_Enzymes_and_Reagents/SCDase

A ceramide-activated protein phosphatase mediates ceramide-induced G<sub>1</sub> arrest of Saccharomyces...A ceramide-activated protein phosphatase mediates ceramide-induced G<sub>1</sub> arrest of Saccharomyces...

A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. / Nickels, Joseph T. ... A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. Genes and Development ... Both a ceramide-activated protein phosphatase and a ceramide-activated protein kinase have been implicated in transmitting the ... Both a ceramide-activated protein phosphatase and a ceramide-activated protein kinase have been implicated in transmitting the ...
more infohttps://pennstate.pure.elsevier.com/en/publications/a-ceramide-activated-protein-phosphatase-mediates-ceramide-induce

The ultrastructural effects and immunolocalisation of fumonisin B1 on cultured oesophageal cancer cells (SNO)The ultrastructural effects and immunolocalisation of fumonisin B1 on cultured oesophageal cancer cells (SNO)

... as So and ceramide.37 Complex sphingolipids have been implicated in cell cell interactions.34 Therefore, a blockage of de novo ... ceramide synthase, which catalyses the acylation of sphinganine (Sa) in the de novo biosynthesis of sphingolipids and the re- ...
more infohttp://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0038-23532009000300015&lng=en&nrm=iso&tlng=en

Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities<...Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities<...

Mass spectrometry revealed elevations of ceramides, hydroxy-ceramides, dihydroceramides, sphingosine, dihexosylceramides, and ... Mass spectrometry revealed elevations of ceramides, hydroxy-ceramides, dihydroceramides, sphingosine, dihexosylceramides, and ... Mass spectrometry revealed elevations of ceramides, hydroxy-ceramides, dihydroceramides, sphingosine, dihexosylceramides, and ... Mass spectrometry revealed elevations of ceramides, hydroxy-ceramides, dihydroceramides, sphingosine, dihexosylceramides, and ...
more infohttps://einstein.pure.elsevier.com/en/publications/acid-ceramidase-deficiency-in-mice-results-in-a-broad-range-of-ce

Strong Heart, Low Ceramides | DiabetesStrong Heart, Low Ceramides | Diabetes

11) identified relationships between dihydroceramides, the precursor to ceramides, that revealed increased rates of ceramide ... Ceramide is upregulated and associated with mortality in patients with chronic heart failure. Can J Cardiol 2015;31:357-363pmid ... Ceramide synthase 5 mediates lipid-induced autophagy and hypertrophy in cardiomyocytes. J Clin Invest 2012;122:3919-3930pmid: ... Plasma ceramides are elevated in female children and adolescents with type 2 diabetes. J Pediatr Endocrinol Metab 2013;26:995- ...
more infohttp://diabetes.diabetesjournals.org/content/67/8/1457

Chemicals, Active / Ceramides - HAPPIChemicals, Active / Ceramides - HAPPI

Ceramides - HAPPI is the industrys leading magazine covering the global personal care, household and industrial and ...
more infohttp://www.happi.com/buyersguide/C_ES/chemicals-active/ceramides/mpo-mag.com/

Hot Ingredient: Ceramides - Skinstore USHot Ingredient: Ceramides - Skinstore US

Using a product containing ceramides replenishes the ceramides in our skin that we have lost over time or due to environmental ... A few of our favorite products that contain ceramides are Elizabeth Arden Ceramide Time Complex Moisture Cream, Kinerase Pro ... As we age, ceramides are also depleted from the skin and our skin can become drier and lose that smooth healthy glow of our ... Ceramides are naturally occurring lipids found in the skin and play a critical role in the barrier function of our skin. If our ...
more infohttps://www.skinstore.com/blog/archive/hot-ingredient-ceramides-2/

Hot Ingredient: Ceramides - Skinstore USHot Ingredient: Ceramides - Skinstore US

What is a ceramide? It is a type of lipid found in the cell membrane of cells. The richest concentration of ceramides are found ... The support provided by ceramides keeps skin firm. However, as we age ceramides decline and skin begins to sag and wrinkle.. ... There are a few causes for the loss of ceramides as we age: Harsh cleansers disrupt and strip ceramides and lipids. Medications ... Dryness, flakiness, irritation, sensitivity, and redness are side effects of ceramide depletion.. In addition, ceramides are ...
more infohttps://www.skinstore.com/blog/archive/hot-ingredient-ceramides/

ceramides | Paulas Choiceceramides | Paula's Choice

Ceramides are naturally occurring, long chains of fats that are major components of skins outer layers. Adding them to a ... On a skincare product ingredient label, youll see those listed as ceramide AP, ceramide EOP, ceramide NG, ceramide NP, ... ceramide NS, phytosphingosine, and sphingosine. The ceramides used in skincare products typically are derived from plants ( ... CLINICAL Ceramide-Enriched Firming Moisturizer. CALM Redness Relief Repairing Serum. About the Experts. Paula Begoun is the ...
more infohttps://www.paulaschoice.com/ingredient-dictionary/skin-replenishing/ceramides.html

Dissecting Ceramide Signals | Science SignalingDissecting Ceramide Signals | Science Signaling

Their experiments suggest that ceramide activates protein kinase C ζ(PKC-ζ) and causes association of PKC-ζ with SAPK and also ... The authors conclude that PKC-ζ mediates effects of ceramide to cause formation of a multiprotein complex that leads to ... Bourbon, N.A., Yun, J., and Kester, M. (2000) Ceramide directly activates protein kinase C to regulate a stress-activated ... Treatment of cells with the inflammatory cytokine interleukin 1 causes synthesis of ceramide, a sphingolipid metabolite that ...
more infohttps://stke.sciencemag.org/content/2000/58/tw6

Medik8 Eyelift | Ceramides | Truth In AgingMedik8 Eyelift | Ceramides | Truth In Aging

Shop our community curated line of eye serums with ceramides, including Medik8 Pretox Eyelift at Truth in Aging. Order today to ... I was hunting around for products with ceramides and came across your review. I enjoyed re-reading this and being reminded of ... Dermaxyl is a patented oligopeptide and ceramide blend that smoothes wrinkles and repairs age-related skin damage. It works by ...
more infohttps://www.truthinaging.com/medik8-pretox-eyelift

Levels of ceramides in the blood help predict cardiovascular events | EurekAlert! Science NewsLevels of ceramides in the blood help predict cardiovascular events | EurekAlert! Science News

Measuring concentrations of a class of lipids known as ceramides in the blood may hold the key to helping clinicians identify ... All cells have the ability to make ceramides; however, ceramide levels tend to accumulate in the blood when we have too much ... Total cholesterol also increased with rising ceramide scores and males were less likely to have high levels of ceramides. ... Levels of ceramides in the blood help predict cardiovascular events Researchers say new marker can help identify patients at ...
more infohttps://www.eurekalert.org/pub_releases/2017-03/acoc-loc030617.php

Ceramides for Skin & Hair: 14 FAQs About Benefits, Use, Side EffectsCeramides for Skin & Hair: 14 FAQs About Benefits, Use, Side Effects

Read on to learn how else your skin might benefit, who needs ceramides most, how to choose the right products, and more. ... ceramides help create a barrier to prevent permeability. This locks moisture into your skin, preventing it from becoming dry ... What are ceramides?. Ceramides are a class of fatty acids called lipids. Theyre naturally found in skin cells and make up ... As long as ceramides are replenished, your skin can benefit. If youre looking for a more "natural" way to induce ceramide ...
more infohttps://www.healthline.com/health/beauty-skin-care/ceramide

Too Cool For School Coconut Ceramide Mask - JCPenneyToo Cool For School Coconut Ceramide Mask - JCPenney

Buy Too Cool For School Coconut Ceramide Mask at JCPenney.com today and enjoy great savings. Available Online Only! ... Lauric acid in coconut water instantly moisturizes and brightens skin, while ceramides protect skin with a healthy moisture ... Formulated with 30 percent coconut water and ceramides, this sheet mask quickly recharges skin with deep and refreshing ... Lightweight and translucent sheet material maximizes delivery of active ingredients in the coconut-ceramide serum into skin, ...
more infohttps://www.jcpenney.com/p/too-cool-for-school-coconut-ceramide-mask/ppr5007296162?pTmplType=sephora

CeramideCeramide

Ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high ... Ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high ... Mechanism by which ceramide signaling occurs. It is hypothesized that after ceramide is generated in the lipid bilayer in the ... Pathways for ceramide synthesis. There are two known means of generating ceramide. The sphingomyelinase pathway uses an enzyme ...
more infohttps://www.bionity.com/en/encyclopedia/Ceramide.html

Ceramide Herbal Eye Cream | Ulta BeautyCeramide Herbal Eye Cream | Ulta Beauty

Mario Badescu Ceramide Herbal Eye Cream is an easily absorbed, non-greasy eye cream made with a blend of vegetable, carnation ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I had a small sample of this and most samples I dont end up buying ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I love this stuff. I have normal/combo skin and this is the kind of ... Comments about Mario Badescu Ceramide Herbal Eye Cream:. I started a new skincare regimen that cause the area around my eyes to ...
more infohttps://www.ulta.com/ceramide-herbal-eye-cream?productId=xlsImpprod6200663

Ceramides Moisturizing & Soothing Skincare Benefits - LOréal ParisCeramides Moisturizing & Soothing Skincare Benefits - L'Oréal Paris

Ceramides maintain skin-hydration & have been suggested to have soothing properties that can alleviate dry skin. ... Ceramides Skin Benefits:. Moisturizing. Soothing. Similar to fatty acids, ceramides are a type of lipids found in cell ... Unfortunately, the amount of ceramides present in the skin decreases with age, which over time can lead to dry and flaky skin. ... A high concentration of ceramides is located in the epidermis- the outermost layer of skin cells. This is to help the skin ...
more infohttps://www.lorealparisusa.com/ingredient-library/ceramides.aspx

Bioactive Ceramides in Health and Disease - Intertwined Roles of Enigmatic Lipids | Johnny Stiban | SpringerBioactive Ceramides in Health and Disease - Intertwined Roles of Enigmatic Lipids | Johnny Stiban | Springer

The focus of this book is the metabolism of ceramide in normal and diseased states and the biophysical and biochemical ... In this book many aspects of ceramide and sphingolipid biology are addressed. ... Many aspects of ceramide and sphingolipid biology are addressed in this book. Its focus is the metabolism of ceramide in normal ... Metabolism of ceramides and their metabolites is also focused with ceramide synthase family of proteins being a target of ...
more infohttps://www.springer.com/gp/book/9783030211615

Ceramides bind VDAC2 to trigger mitochondrial apoptosis - Research database - University of GroningenCeramides bind VDAC2 to trigger mitochondrial apoptosis - Research database - University of Groningen

Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Dadsena, S., Bockelmann, S., Mina, J. G. M., Hassan, D. G., Korneev, S ... Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. ... Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ... Ceramides bind VDAC2 to trigger mitochondrialapoptosis Final publishers version, 2 MB, PDF-document ...
more infohttps://www.rug.nl/research/portal/publications/ceramides-bind-vdac2-to-trigger-mitochondrial-apoptosis

Browse Products Containing: CERAMIDE 6-II || Skin Deep® Cosmetics Database | EWGBrowse Products Containing: CERAMIDE 6-II || Skin Deep® Cosmetics Database | EWG

Dancing Dingo Triple Ceramide Collagen Serum. Anti-aging, Facial Moisturizer/treatment, Serums & Essences. ... OLD PRODUCT: Dancing Dingo Triple Ceramide Collagen Serum (old formulation). Facial Moisturizer/treatment. ...
more infohttp://www.ewg.org/skindeep/browse.php?comp_id=378&ingred06=701206

Elizabeth Arden Ceramide purifying toner 200ml | DebenhamsElizabeth Arden 'Ceramide' purifying toner 200ml | Debenhams

Phase two milky contains- Ceramide III to soothe and calm skin. ... Phase two milky contains- Ceramide III to soothe and calm ...
more infohttps://www.debenhams.com/webapp/wcs/stores/servlet/prod_10701_10001_123041915099_-1

Orgasol® Restore - Multifunctional powder with replenishing ceramides - ArkemaOrgasol® Restore - Multifunctional powder with replenishing ceramides - Arkema

Restore brings the original efficacy of ceramides into a multifunctional powder. ... Orgasol® Restore - Multifunctional powder with replenishing ceramides. Product range. Designed to impart sensory appeal, ... Together with cholesterol and saturated fatty acids, ceramides are essential components of the epidermis. They help prevent ... Restore brings the original efficacy of ceramides into a multifunctional powder. ...
more infohttps://www.arkema.com/en/products/product-finder/range-viewer/Orgasol-Restore-Multifunctional-powder-with-replenishing-ceramides/

Ceramide - WikipediaCeramide - Wikipedia

Ceramides are a family of waxy lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in ... The word ceramide comes from the Latin cera (wax) and amide. Ceramide is a component of vernix caseosa, the waxy or cheese-like ... Ceramide is subsequently transported to the Golgi apparatus by either vesicular trafficking or the ceramide transfer protein ... "Ceramides - Skin Lipids That Keep Skin Moisturized". Retrieved 29 January 2015. "Safety Assessment of Ceramides as Used in ...
more infohttps://en.wikipedia.org/wiki/Ceramide

Ceramide Skin Care | Elizabeth ArdenCeramide Skin Care | Elizabeth Arden

Browse our complete collection of ceramide products. Elizabeth Ardens ceramides for facial skin help retain moisture to reduce ... Ceramides. Crucial to maintaining your moisture barrier, skins first defense against aging signs. ...
more infohttp://www.elizabetharden.com/skincare/ceramide/?prefn1=skinType&prefv1=allTypes
  • Measuring concentrations of a class of lipids known as ceramides in the blood may hold the key to helping clinicians identify individuals with suspected coronary heart disease who need treatment or should be followed more closely, according to research scheduled for presentation at the American College of Cardiology's 66th Annual Scientific Session. (eurekalert.org)
  • Plasma ceramides are a class of lipids that are highly linked to cardiovascular disease processes. (medindia.net)
  • Certain cancer cells have shown an increased level of ceramides and with the help of these protein interactions, the progression and establishment of cancer can be analyzed. (medindia.net)
  • Lipotoxic cardiomyopathy (LCM) is a heart condition that usually occurs in patients with diabetes and obesity due to the accumulation of a type of fat known as ceramides, finds a study conducted at Sanford Burnham Prebys Medical Discovery Institute (SBP). (medindia.net)
  • Other groups have found relationships between circulating ceramides and future cardiovascular events. (diabetesjournals.org)
  • Study data show that ceramides were able to predict major cardiovascular events--heart attack, stroke, revascularization (a procedure or surgery to open blocked arteries) and death--among patients with and without evidence of blockages and in those with low LDL, often relied on as the gold standard for benchmarking risk. (eurekalert.org)
  • Mayo Clinic has launched a new type of blood test that will measures blood concentrations of plasma ceramides to predict adverse cardiovascular events in patients with progressing coronary artery disease (CAD). (medindia.net)
  • The researchers also found higher versus lower ceramides risk scores associated with increased risk of cardiovascular events, even in participants with no evidence of CAD by angiography and in those with low LDL-C levels, 7.8% versus 2.2% and 16.4% versus 3.7% respectively. (aacc.org)
  • These ceramides affect the molecules through various mechanisms and ultimately lead to apoptosis (programmed cell death) and heart muscle protein instability. (medindia.net)
  • We highly suggest First Aid Beauty Ultra Repair Cream that incorporates ceramides with shea butter and colloidal oatmeal to produce an amazing calming, smoothing, hydrating cream that reduce symptoms of eczema, dermatitis, and keratosis pilaris. (skinstore.com)
  • Over the past 20 years, a large number of studies in preclinical models suggest that ceramides may be among the most pathogenic nutrient metabolites that accumulate in obesity, linking overnutrition to insulin resistance and its sequelae of comorbidities. (diabetesjournals.org)
  • These studies are consistent with earlier, smaller studies evaluating relationships between serum ceramides and insulin resistance in both humans and nonhuman primates ( 6 - 10 ). (diabetesjournals.org)
  • Based on our findings, measuring ceramides in the blood appears to be a new, potentially better marker than LDL in predicting first and repeat cardiac events in both patients with and without established coronary blockages," said Jeff Meeusen, PhD, a clinical chemist and co-director of Cardiovascular Laboratory Medicine at Mayo Clinic, and the study's lead author. (eurekalert.org)
  • Lipotoxic cardiomyopathy (LCM) is caused due to the accumulation of ceramides. (medindia.net)
  • Lipotoxic cardiomyopathy (LCM) is caused by the accumulation of ceramides, which occurs due to the consumption of a diet high in fat or high in calories. (medindia.net)
  • The findings point to ceramides as a "potentially better marker than LDL-C in predicting first and repeat cardiac events," according to the study's first author, Jeff Meeusen, PhD, co-director of cardiovascular laboratory medicine at Mayo Clinic in Rochester, Minnesota. (aacc.org)
  • Despite the strongly consistent findings obtained in preclinical models, the role of ceramides in human cardiometabolic pathologies has been controversial. (diabetesjournals.org)
  • Designed to impart sensory appeal, instant smoothing and regenerative benefits to stressed and mature skin, Orgasol ® Restore brings the original efficacy of ceramides into a multifunctional powder. (arkema.com)
  • Ceramides work synergistically to help increase skin hydration and promote optimal barrier function. (skinceuticals.com)
  • Here, we summarize these results in the context of other preclinical and clinical studies investigating roles for ceramides as drivers of cardiometabolic dysfunction. (diabetesjournals.org)
  • This book is about the various roles of bioactive ceramides and other sphingolipids in cellular biology. (springer.com)
  • In addition, topical application of products containing ceramides have been suggested to provide soothing properties that can help to alleviate dry skin. (lorealparisusa.com)
  • Topical ceramides are generally considered safe. (healthline.com)
  • Ceramides play an important part of your skin's role as a protective barrier, helping to bind together the cells that make up the surface of your skin.These nourishing oils help retain moisture and protect your skin. (victoriahealth.com)
  • This prohydration maintenance complex boosts skin s ability to do both* Ceramides lock in moisture at skin s surface and retain hydration. (influenster.com)
  • Ceramosides Ceramides infuse skin with moisture-locking lipids that bump up hydration for skin that s softer and smoother. (influenster.com)
  • Here, we'll dive deep to clarify how ceramides help skin retain moisture, fortify its protective barrier, and keep its appearance firm and plump-especially given the recent research clarifying ceramides' unique ability to rejuvenate skin's appearance. (paulaschoice.com)
  • The enigmatic biophysical and biochemical properties of ceramides and their propensity to influence membranes whether as rafts or protein-permeable channels are heavily discussed. (springer.com)
  • 11 ) identified relationships between dihydroceramides, the precursor to ceramides, that revealed increased rates of ceramide synthesis as markers for and predictors of diabetes development. (diabetesjournals.org)
  • The research gives direct evidence of the association between ceramides and heart dysfunction and also helps to decrease LCM symptoms by inhibiting ceramide synthesis. (medindia.net)
  • CerS are involved in the de novo synthesis pathway of ceramides. (wikipedia.org)
  • 1 ) are interesting to consider in relation to other recent studies evaluating relationships between ceramides and clinical indices of cardiometabolic dysfunction. (diabetesjournals.org)
  • Can ceramides be combined with other skin care ingredients for maximum effect? (healthline.com)
  • Using ceramides in combination with other skin care ingredients may help you better achieve your desired results. (healthline.com)
  • As far as anti-aging ingredients go, ceramides have a proven record, but are often overlooked, and rarely explained. (paulaschoice.com)
  • For example, phytosphingosine and sphingosine are ingredients known as ceramide precursors, meaning they can nudge skin into making ceramides. (paulaschoice.com)
  • Ceramides fall under the category of "skin-replenishing" (aka skin-identical) ingredients. (paulaschoice.com)
  • Which other ingredients do ceramides work well with? (paulaschoice.com)
  • Plasma ceramides are elevated in female children and adolescents with type 2 diabetes. (nih.gov)
  • Ceramides play an important role. (skinstore.com)
  • While ceramides are noted for their role in brain and nervous system development, they've gained a lot of interest in the skin care world for their potential skin health benefits. (healthline.com)
  • Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity. (rug.nl)
  • Ceramides play a complicated role in the heart. (medindia.net)
  • It proved that ceramides play a central role in LCM. (medindia.net)
  • Wheat contains these same ceramides, but you can't get enough from wheat in your diet to make a difference. (victoriahealth.com)
  • The studies showed that feeding the flies with ceramides and lowering the gene expression that break down lipids have similar, negative effects. (medindia.net)
  • Researchers measured four different types of ceramides in the blood at baseline and combined the values into a 12-point scale. (eurekalert.org)
  • Ceramides 2 and 3 are widely used in products designed for the face and the neck. (healthline.com)
  • The ceramides in most skin care products are synthetically made. (healthline.com)
  • If my skin already contains ceramides, why do I need them in my beauty products? (paulaschoice.com)
  • What results will I see from products containing ceramides? (paulaschoice.com)
  • But, given ceramides' stellar anti-aging abilities, most products will advertise them on their labels or product pages if they're included in the formula. (paulaschoice.com)
  • His research is focused on ceramides and their relevance in human biology, mainly in cancer and other diseases. (springer.com)
  • Exactly where you include ceramides in your skin care routine depends on the type of product you're using. (healthline.com)
  • When it comes to ceramides, not all product packaging is created equal. (healthline.com)
  • If you're looking for a product to heal dry, irritated skin, you can look for one that has ceramides 1, 3, or 6-II . (healthline.com)
  • How will I know if a product contains ceramides? (paulaschoice.com)
  • In contrast to LDL-C's artery-clogging properties, ceramides actively attract inflammatory cells and promote clotting, Meeusen explained in a prepared statement. (aacc.org)
  • Think of ceramides as the mortar between bricks-if the bricks are your skin cells. (paulaschoice.com)