Amidohydrolases that are specific for the cleavage of the N-acyl linkage of CERAMIDES. Ceramidases are classified as acidic, neutral or basic according to the optimal pH with which they function.
A ceramidase subtype that is active at alkaline pH. It is found at high levels within the SMALL INTESTINE.
A ceramidase subtype that is active at neutral pH. It is found at high levels within the SMALL INTESTINE and in the BRAIN.
A ceramidase subtype that is active at acid pH. It plays an important role in sphingolipid degradation by catalyzing the lysosomal hydrolysis of ceramide to sphingosine and free fatty acid. Inherited deficiency of acid ceramidase activity results in FARBER LIPOGRANULOMATOSIS.
Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE.
An enzyme that catalyzes the hydrolysis of a ceramidetrihexoside to a ceramidedihexoside plus galactose.
An amino alcohol with a long unsaturated hydrocarbon chain. Sphingosine and its derivative sphinganine are the major bases of the sphingolipids in mammals. (Dorland, 28th ed)
Derivatives of PHOSPHATIDIC ACIDS that lack one of its fatty acyl chains due to its hydrolytic removal.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
A sphingolipidosis subtype that is characterized by the histological appearance of granulomatous deposits in tissues. It results from the accumulation of CERAMIDES in various tissues due to an inherited deficiency of ACID CERAMIDASE.
The chemical reactions involved in the production and utilization of various forms of energy in cells.
A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)
The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
Cell surface receptors for ADIPONECTIN, an antidiabetic hormone secreted by ADIPOCYTES. Adiponectin receptors are membrane proteins with multiple cytoplasmic and extracellular regions. They are about 43 kDa and encoded by at least two genes with different affinities for globular and full-length adiponectin.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12.
Tumors or cancer of the human BREAST.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)
Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.
The process of cleaving a chemical compound by the addition of a molecule of water.
A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.
A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)
A ganglioside present in abnormally large amounts in the brain and liver due to a deficient biosynthetic enzyme, G(M3):UDP-N-acetylgalactosaminyltransferase. Deficiency of this enzyme prevents the formation of G(M2) ganglioside from G(M3) ganglioside and is the cause of an anabolic sphingolipidosis.

Increases in ceramide levels in normal human mesangial cells subjected to different cellular stresses result from changes in distinct enzyme activities and can influence cellular responses to other stimuli. (1/125)

Sphingolipids, ceramide in particular, have come to be regarded as having roles in cellular signaling, most recently being associated with stress and the cellular responses to stress. In the present study we first examined the mechanisms involved in the changes in cellular ceramide levels in normal human mesangial cells (NHMC) in the growth, quiescent, and senescent phases as well as those resulting from environmental stimuli. We found that in NHMC total ceramide levels increase in response to cellular stresses as a result of a combination of enzyme activities. Furthermore, different stresses cause different alterations in various enzyme activities, with age and growth influencing acidic enzymes, but cell density affecting neutral, resulting in final ceramide level increases which most likely are associated with distinct pools of ceramide. Secondly, we examined the influence of changes in ceramide levels on apoptosis induced by sphingosine and its methylated derivative N, N-dimethylsphingosine. We found that increases in cellular ceramide levels prohibited the apoptosis and caused a quiescent state in the cells. The data presented here provide additional insight into the roles of ceramide and related enzymes in cellular responses to stress and suggest a possible relevance to in vivo disease states.  (+info)

Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. (2/125)

A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases.  (+info)

Ceramide-induced killing of normal and malignant human lymphocytes is by a non-apoptotic mechanism. (3/125)

Synthetic ceramides induce apoptotic death of Jurkat and HL60 leukaemia cell lines. By contrast we show here that ceramide induces non-apoptotic killing of malignant cells from patients with B-chronic lymphocytic leukaemia (B-CLL) and of normal B lymphocytes. The protein phosphatase inhibitor okadaic acid readily induces apoptosis of B-CLL cells, indicating that this death pathway is fully functional in these cells. The ability of ceramide to activate the apoptotic protease caspase 3 in HL60 cells but not in B-CLL cells, as well as the lack of correlation of ceramide-mediated killing of different B-CLL isolates with expression of the apoptosis-regulating proteins bcl-2 and bax reinforce the conclusion that ceramide killing of B-CLL cells is by a non-apoptotic mechanism. Fludarabine treatment or gamma-irradiation of B-CLL cells resulted in ceramide elevation and in killing by both apoptotic and non-apoptotic mechanisms, suggesting that a ceramide-triggered non-apoptotic mechanism may play a role in the killing of these cells. Therefore, the results here show that ceramide can induce either apoptotic or non-apoptotic death, depending on the cellular context. The inability of synthetic dihydroceramide to kill B-CLL cells or normal B lymphocytes suggests that non-apoptotic killing by ceramide is via interaction with a specific, but unidentified, cellular target.  (+info)

Role of sphingosine 1-phosphate in the mitogenesis induced by oxidized low density lipoprotein in smooth muscle cells via activation of sphingomyelinase, ceramidase, and sphingosine kinase. (4/125)

Oxidized LDL (oxLDL) have been implicated in diverse biological events leading to the development of atherosclerotic lesions. We previously demonstrated that the proliferation of cultured vascular smooth muscle cells (SMC) induced by oxLDL is preceded by an increase in neutral sphingomyelinase activity, sphingomyelin turnover to ceramide, and stimulation of mitogen-activated protein kinases (Auge, N., Escargueil-Blanc, I., Lajoie-Mazenc, I., Suc, I., Andrieu-Abadie, N., Pieraggi, M. T., Chatelut, M., Thiers, J. C., Jaffrezou, J. P., Laurent, G., Levade, T., Negre-Salvayre, A., and Salvayre, R. (1998) J. Biol. Chem. 273, 12893-12900). Since ceramide can be converted to other bioactive metabolites, such as the well established mitogen sphingosine 1-phosphate (S1P), we investigated whether additional ceramide metabolites are involved in the oxLDL-induced SMC proliferation. We report here that incubation of SMC with oxLDL increased the activities of both acidic and alkaline ceramidases as well as sphingosine kinase, and elevated cellular sphingosine and S1P. Furthermore, the mitogenic effect of oxLDL was inhibited by D-erythro-2-(N-myristoylamino)-1-phenyl-1-propanol and N,N-dimethylsphingosine which are inhibitors of ceramidase and sphingosine kinase, respectively. These findings suggest that S1P is a key mediator of the mitogenic effect of oxLDL. In agreement with this conclusion, exogenous addition of sphingosine stimulated the proliferation of cultured SMC, and this effect was abrogated by dimethylsphingosine but not by fumonisin B1, an inhibitor of the acylation of sphingosine to ceramide. Exogenous S1P also promoted SMC proliferation. Altogether, these results strongly suggest that the mitogenic effect of oxLDL in SMC involves the combined activation of sphingomyelinase(s), ceramidase(s), and sphingosine kinase, resulting in the turnover of sphingomyelin to a number of sphingolipid metabolites, of which at least S1P is critical for mitogenesis.  (+info)

Purification and characterization of a membrane-bound nonlysosomal ceramidase from rat brain. (5/125)

We have purified a membrane bound ceramidase 22,300-fold to apparent homogeneity. The purification scheme included Triton X-100 extraction of membranes followed by Q-Sepharose, blue Sepharose, phenyl-Sepharose, and MonoS column chromatography. The purified enzyme showed an apparent molecular mass of 90 kDa as estimated by SDS-polyacrylamide gel electrophoresis under reducing conditions and 95 kDa by chromatography on Superose 12. Using C(16)-ceramide as substrate, the enzyme showed a broad pH optimum in the neutral to alkaline range. A mixed micelle assay was developed, and using Triton X-100/ceramide mixed micelles, the enzyme exhibited classical Michaelis-Menten kinetics, with a K(m) of 1.29 mol % and a V(max) of 4.4 micromol/min/mg. When dihydroceramide was used as substrate, these values were 3.84 mol % and 1.2 micromol/min/mg, respectively, indicating that the enzyme hydrolyzes ceramides preferentially. The activity of the purified ceramidase did not require cations, and it was inhibited by reducing agents. Phosphatidylcholine and sphingomyelin were without effect on the enzyme activity, whereas phosphatidic acid and phosphatidylserine stimulated the activity 3-fold. Sphingosine acted as a competitive inhibitor with an IC(50) of 5-10 microM. These results indicate that the purified enzyme is a novel ceramidase.  (+info)

Activation of sphingosine kinase by tumor necrosis factor-alpha inhibits apoptosis in human endothelial cells. (6/125)

Human umbilical vein endothelial cells (HUVEC), like most normal cells, are resistant to tumor necrosis factor-alpha (TNF)-induced apoptosis in spite of TNF activating sphingomyelinase and generating ceramide, a known inducer of apoptosis. Here we report that TNF activates another key enzyme, sphingosine kinase (SphK), in the sphingomyelin metabolic pathway resulting in production of sphingosine-1-phosphate (S1P) and that S1P is a potent antagonist of TNF-mediated apoptosis. The TNF-induced SphK activation is independent of sphingomyelinase and ceramidase activities, suggesting that TNF affects this enzyme directly other than through a mass effect on sphingomyelin degradation. In contrast to normal HUVEC, in a spontaneously transformed endothelial cell line (C11) TNF stimulation failed to activate SphK and induced apoptosis as characterized by morphological and biochemical criteria. Addition of exogenous S1P or increasing endogenous S1P by phorbol ester markedly protected C11 cell line from TNF-induced apoptosis. Conversely, N, N-dimethylsphingosine, an inhibitor of SphK, profoundly sensitized normal HUVEC to killing by TNF. Thus, we demonstrate that the activation of SphK by TNF is an important signaling for protection from the apoptotic effect of TNF in endothelial cells.  (+info)

Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis. (7/125)

We previously reported the purification and characterization of a novel type of alkaline ceramidase from Pseudomonas aeruginosa strain AN17 (Okino, N., Tani, M., Imayama, S., and Ito, M. (1998) J. Biol. Chem. 273, 14368-14373). Here, we report the molecular cloning, sequencing, and expression of the gene encoding the ceramidase of this strain. Specific oligonucleotide primers were synthesized using the peptide sequences of the purified ceramidase obtained by digestion with lysylendopeptidase and used for polymerase chain reaction. DNA fragments thus amplified were used as probes to clone the gene encoding the ceramidase from a genomic library of strain AN17. The open reading frame of 2,010 nucleotides encoded a polypeptide of 670 amino acids including a signal sequence of 24 residues, 64 residues of which matched the amino acid sequence determined for the purified enzyme. The molecular weight of the mature enzyme was estimated to be 70,767 from the deduced amino acid sequence. Expression of the ceramidase gene in Escherichia coli, resulted in production of a soluble enzyme with the identical N-terminal amino acid sequence. Recombinant ceramidase was purified to homogeneity from the lysate of E. coli cells and confirmed to be identical to the Pseudomonas enzyme in its specificity and other enzymatic properties. No significant sequence similarities were found in other known functional proteins including human acid ceramidase. However, we found a sequence homologous to the ceramidase in hypothetical proteins encoded in Mycobacterium tuberculosis, Dictyostelium discoideum, and Arabidopsis thaliana. The homologue of the ceramidase gene was thus cloned from an M. tuberculosis cosmid and expressed in E. coli, and the gene was demonstrated to encode an alkaline ceramidase. This is the first report for the cloning of an alkaline ceramidase.  (+info)

Purification and characterization of a neutral ceramidase from mouse liver. A single protein catalyzes the reversible reaction in which ceramide is both hydrolyzed and synthesized. (8/125)

We report here a novel ceramidase that was purified more than 150, 000-fold from the membrane fraction of mouse liver. The enzyme was a monomeric polypeptide having a molecular mass of 94 kDa and was highly glycosylated with N-glycans. The amino acid sequence of a fragment obtained from the purified enzyme was homologous to those deduced from the genes encoding an alkaline ceramidase of Pseudomonas aeruginosa and a hypotheical protein of the slime mold Dictyostelium discoideum. However, no significant sequence similarities were found in other known functional proteins including acid ceramidases of humans and mice. The enzyme hydrolyzed various N-acylsphingosines but not galactosylceramide, sulfatide, GM1a, or sphingomyelin. The enzyme exhibited the highest activity around pH 7.5 and was thus identified as a type of neutral ceramidase. The apparent K(m) and V(max) values for C12-4-nitrobenzo-2-oxa-1, 3-diazole-ceramide and C16-(14)C-ceramide were 22.3 microM and 29.1 micromol/min/mg and 72.4 microM and 3.6 micromol/min/mg, respectively. This study also clearly demonstrated that the purified 94-kDa ceramidase catalyzed the condensation of fatty acid to sphingosine to generate ceramide, but did not catalyze acyl-CoA-dependent acyl-transfer reaction.  (+info)

Our long-term goal is to define the mechanisms by which anti-cancer chemotherapeutic agents (ACCAs) induce anti-proliferative responses (proliferation inhibitio...
Ceramide plays an important role in regulating cell proliferation and apoptosis. Recent studies indicate that generation of ceramide in the intestine from sphingomyelin hydrolysis may be implicated in colon cancer development. The enzymes that catalyze the further hydrolysis of ceramide in the intestine have, however, not been well investigated. Our data reveal the existence of a ceramidase (EC 3.5.1.23) in rat intestinal mucosa with an optimal pH of 7.0. One milligram of mucosal protein is able to hydrolyze 44.0 ± 9.6 nmol of ceramide in 1 hr. The activity is low in the proximal duodenum and increases to a plateau in the proximal jejunum. The activity is then similar throughout the small intestine, until it declines in the distal part of ileum. Some activity is also detectable in the colon. The activity increases slightly in the presence of monomeric bile salt concentrations and sharply at the critical micellar concentration. Similar patterns were observed for both primary (taurocholate) and ...
Compare neutral ceramidase ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
<p>Modification of the basic amino alcohol backbones gives rise to a large family of sphingolipids that play important roles in membrane biology and provide many bioactive metabolites that regulate cell function. This large family of sphingolipids varies in structure and function, but their creation and destruction are governed by common synthetic and catabolic pathways. They arise from a single entry point and converge to a final breakdown pathway. Metabolites of sphingolipids are responsible for various signaling and regulatory roles within cells. These metabolites are also linked to many diseases. Inhibitors of sphingolipid metabolism enzymes have been widely studied and investigated as possible drugs.</p> <p> Canals, D., Perry, D. M., Jenkins, R. W., &amp; Hannun, Y. A. (2011). Drug targeting of sphingolipid metabolism: sphingomyelinases and ceramidases. <i>British journal of pharmacology</i>, <i>163</i>(4), 694-712. <a href="https://doi.org/10
Erika Espinoza of Free Hoop is sharing this useful little tutorial for all of our Spanish-speaking hoopers out there who want to give it a go and make their own hula hoop! Her recommendations for materials are mostly stuff you can get at your local hardware store and you need no more than your kitchen and some space to make it all happen. So go for it and try making your own hula! Erika lives Monterrey, Nuevo Leon, Mexico.. Erika Espinoza de Free Hoop nos comparte este pequeño tutorial, super útil para todos nuestros hoopers de habla hispana que quisieran intentar hacer sus propios aros hula hula. Sus recomendaciones para los materiales son en su mayoría cosas que puedes conseguir en la ferretería o tienda de materiales más cercana. No necesitas más que tu cocina y un espacio para hacer que todo suceda. Así que, inténtalo y estamos seguros que te sentirás [email protected] de hacer tu propia hula. Erika vive Monterrey, Nuevo León, México.. ...
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Glioblastoma is considered as the most aggressive form of brain tumor with overall survival less than fifteen months after diagnosis, even with multimodal therapy. It constitutes a major percentage of malignant brain tumors, with over 10,000 new cases reported every year in the USA. Comprehensive proteomic study of the disease in our lab has revealed that acid ceramidase is a novel target for possible drug development. Previous in Vitro study has found that acid ceramidase inhibitors (e.g. carmofur) have better efficacy than the standard chemotherapeutic drug available. This study aims to identify the role of acid ceramidase in glioblastoma and the efficacy of acid ceramidase inhibitors in suppressing the tumor, which may potentially lead to the development of a new chemotherapeutic agent for treating glioblastoma. Herein, we have worked towards the development of xenografted zebrafish (Danio rerio) glioblastoma model. For which, we have developed a zebrafish experimental microinjection protocol, where
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The epidermis is the outermost layer of skin that acts as a barrier to protect the body from the external environment and to control water and heat loss. This barrier function is established through the multistage differentiation of keratinocytes and the presence of bioactive sphingolipids such as c …
The adiponectin receptor 1 for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (Tanabe et al. 2015; Yamauchi et al. 2003. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin. The 3-d structure revealed ceramidase activity for both ADIPOR1 and ADIPOR2; however, the two structures are substantially different (Vasiliauskaité-Brooks et al. 2017). It may function with adiponectin to stimulate cholesterol efflux via ABCA1 (Hafiane et al. 2019 ...
The adiponectin receptor 1 for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (Tanabe et al. 2015; Yamauchi et al. 2003. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin. The 3-d structure revealed ceramidase activity for both ADIPOR1 and ADIPOR2; however, the two structures are substantially different (Vasiliauskaité-Brooks et al. 2017 ...
A research group that previously showed that overexpression of ceramidase (CDase) in Drosophila photoreceptors suppressed retinal degeneration in response to certain mutations has now made the intriguing discovery that CDase regulation of photoreceptor function is cell nonautonomous. CDases, which convert ceramide to sphingosine, play a key role in metabolism of the sphingolipids, a lipid family that comprises not only structural components of cell membranes but also signaling molecules. Acharya et al. created flies in which almost the entire eye was homozygous for a null mutation of CDase, whereas other regions were CDase heterozygous. Ultrastructural analysis revealed that the photoreceptors of the mosaic flies were structurally normal; furthermore, immunocytochemical analysis of eye discs and Western blot analysis of retinal extracts revealed CDase protein. Overexpression of CDase in the fat body or in mushroom body neurons (tissues distinct from the retina) rescued photoreceptor degeneration ...
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Supplementary MaterialsVideo S1. Apical ECM/BM effects modeled as viscous resistances and exterior viscous level of resistance coefficient put on both areas at 8,000?Pa s m?1. Range bar is normally 20?m. Simulation period depicted over the structures. mmc3.mp4 (2.0M) GUID:?63F04E92-06A0-488F-8AD5-FA7674E03304 Video S3. Appropriate Flip Morphology Emerges with Planar Differential Development Prices and an 1187594-09-7 Explicit Description …Read More. ...
Supplementary MaterialsSupp FigS2. copy number of the mtDNA genes and by qPCR in RVH individuals before and 24hrs after PTRA, performed during IV infusion of vehicle (n=8) or the mitoprotective drug elamipretide (ELAM, 0.05mg/kg/hr, n=6). Five healthy volunteers (HV) served as settings. Urinary mtDNA levels YM-90709 were also assessed in RVH and normal pigs (n=7 …Read More. ...
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TY - JOUR. T1 - Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities. AU - Sikora, Jakub. AU - Dworski, Shaalee. AU - Jones, E. Ellen. AU - Kamani, Mustafa A.. AU - Micsenyi, Matthew C.. AU - Sawada, Tomo. AU - Le Faouder, Pauline. AU - Bertrand-Michel, Justine. AU - Dupuy, Aude. AU - Dunn, Christopher K.. AU - Xuan, Ingrid Cong Yang. AU - Casas, Josefina. AU - Fabrias, Gemma. AU - Hampson, David R.. AU - Levade, Thierry. AU - Drake, Richard R.. AU - Medin, Jeffrey A.. AU - Walkley, Steven U.. PY - 2017/4/1. Y1 - 2017/4/1. N2 - Farber disease is a rare autosomal recessive disorder caused by acid ceramidase deficiency that usually presents as early-onset progressive visceral and neurologic disease. To understand the neurologic abnormality, we investigated behavioral, biochemical, and cellular abnormalities in the central nervous system of Asah1P361R/P361R mice, which serve as a model of Farber disease. Behaviorally, the mutant mice had reduced ...
Farber disease (also known as Farbers lipogranulomatosis, ceramidase deficiency, Fibrocytic dysmucopolysaccharidosis, and Lipogranulomatosis[1]:545) is an extremely rare (80 cases reported worldwide to this day[2]) autosomal recessive lysosomal storage disease marked by a deficiency in the enzyme ceramidase that causes an accumulation of fatty material sphingolipids leading to abnormalities in the joints, liver, throat, tissues and central nervous system. Normally, the enzyme ceramidase breaks down fatty material in the bodys cells. In Farber Disease, the gene responsible for making this enzyme is mutated. Hence, the fatty material is never broken down and, instead, accumulates in various parts of the body, leading to the signs and symptoms of this disorder. ...
This invention provides a novel ganglioside ceramidase which has a substrate specificity at least for GD1a, GM1, GM2 and GM3 and acts at least on GD1a, GM1, GM2 and GM3 and catalyzes the reaction of hydrolysis of ganglioside to lysoganglioside and fatty acid.This invention further provides a process for producing the novel ganglioside ceramidase which comprises cultivating ganglioside ceramidase producing strain belonging to the genus Nocardia in a culture medium and collecting ganglioside ceramidase from the culture.
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FUNCTION: This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype. [provided by RefSeq, Oct 2015 ...
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The KRAB-zinc finger proteins (KRAB-ZFPs) represent an extremely large, but poorly understood, family of transcriptional regulators in mammals. (Sera) cells, endogenous KRAB-ZFPs do not. Rather, KRAB-ZFPs and KAP1 relocalise to novel nucleoplasmic foci that we possess termed KRAB- and KAP1-connected (KAKA) foci. HP1s can also concentrate in these foci and there is a close spatial […]. ...
2009/3/6 Marcelo Guzmán ,marcegl91 at gmail.com,: ,, Evitar el top-posting está entre las sugerencias, no entre las reglas. , , De hecho, no. Se obliga al user a no hacer top-posting. Ojo, eh!. Yo estoy de acuerdo con NO hacer top posting..., pero es falso que es una sugerencia, son las reglas de la lista, y como ellas mismas, deben ser respetadas. Marcelo: Acá tenés las reglas y las sugerencias. Te recomiendo que las leas: https://wiki.ubuntu.com/ArgentinaTeam/EtiquetaML Idem para el compañero Hugo Valencia. No seamos más estrictos que la propia etiqueta. Querámosnos. Saludos Moski ...
RESUMEN. Introducci n: La artroplastia reversa es en la actualidad la artroplastia de hombro con m s indicaciones y capacidad de soluci n de problemas. De todas las artroplastias reversas de hombro (ARH) m s de 50% habr n fallado a los 10 a os. El objetivo de esta revisi n de alcance es identificar las principales problem ticas a las cuales nos enfrentamos al tener un paciente con una pr tesis reversa fallida. Material y m todos: Se realiz una revisi n de alcance del tema fallo de pr tesis reversa y revisi n de pr tesis reversa de hombro . Debido a la reciente aparici n de este tipo de problemas y a su constante cambio en el manejo, se decidi realizarla de la literatura de los ltimos cinco a os (diciembre de 2014 a noviembre de 2019). Resultados: Las principales causas de revisi n de la ARH son inestabilidad (32%), infecci n (13.8%), disociaci n del componente glenoideo (12.2%), aflojamiento glenoideo (10.4%), aflojamiento humeral (5.5%), dificultad al poner la base glenoidea (4.8%) y dificultad ...
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The ASAH1 Gene codes for acid ceramidase which is an enzyme found in lysosomes. The lysosome breaks down acid ceramidase and ... The lack of myelin resulting from the lack of acid ceramidase break down leads to nerve cell damage. Spinal muscular atrophies ... Park, Jae-Ho; Schuchman, Edward H. (December 2006). "Acid ceramidase and human disease". Biochimica et Biophysica Acta (BBA) - ... "Acid ceramidase deficiency associated with spinal muscular atrophy with progressive myoclonic epilepsy". Neuromuscular ...
Okino N, He X, Gatt S, Sandhoff K, Ito M, Schuchman EH (August 2003). "The reverse activity of human acid ceramidase". The ... Seelan RS, Qian C, Yokomizo A, Bostwick DG, Smith DI, Liu W (October 2000). "Human acid ceramidase is overexpressed but not ... Bär J, Linke T, Ferlinz K, Neumann U, Schuchman EH, Sandhoff K (March 2001). "Molecular analysis of acid ceramidase deficiency ... The ASAH1 gene encodes in humans the acid ceramidase enzyme. This gene encodes a heterodimeric protein consisting of a ...
Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene. GRCh38: Ensembl release 89: ENSG00000188611 - ... 2004). "Neutral ceramidase gene: role in regulating ceramide-induced apoptosis". Gene. 315: 113-22. doi:10.1016/S0378-1119(03) ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ... Overview of all the structural information available in the PDB for UniProt: Q9NR71 (Neutral ceramidase; ASAH2) at the PDBe-KB ...
Alkaline ceramidase 3 also known as ACER3 is a ceramidase enzyme which in humans is encoded by the ACER3 gene. GRCh38: Ensembl ... Mao C, Obeid LM (September 2008). "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and ... "Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide". J. Biol. ...
Neutral ceramidase C also known as N-acylsphingosine amidohydrolase 2C or non-lysosomal ceramidase C or ASAH2C is a ceramidase ... Hwang YH, Tani M, Nakagawa T, Okino N, Ito M (May 2005). "Subcellular localization of human neutral ceramidase expressed in ... Choi MS, Anderson MA, Zhang Z, Zimonjic DB, Popescu N, Mukherjee AB (October 2003). "Neutral ceramidase gene: role in ... "Identification of a novel amidase motif in neutral ceramidase". The Biochemical Journal. 393 (Pt 3): 687-95. doi:10.1042/ ...
2005). "Elevation of the level and activity of acid ceramidase in Alzheimer's disease brain". Eur. J. Neurosci. 20 (12): 3489- ... This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript ... "Entrez Gene: ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like". Human NAAA genome location and NAAA gene details ... Schulze H, Schepers U, Sandhoff K (2008). "Overexpression and mass spectrometry analysis of mature human acid ceramidase". Biol ...
Alkaline ceramidase 2 also known as ACER2 is a ceramidase enzyme which in humans is encoded by the ACER2 gene. The ACER2/ ... Sun W, Hu W, Xu R, Jin J, Szulc ZM, Zhang G, Galadari SH, Obeid LM, Mao C (February 2009). "Alkaline ceramidase 2 regulates ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ... Mao C, Obeid LM (September 2008). "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and ...
Sphingosine (Sph) is formed by the action of ceramidase (CDase) enzymes on ceramide in the lysosome. Sph can also be formed in ... The low levels of Sph and their increase in response to stimulation of cells, primarily by activation of ceramidase by growth- ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ... Ceramide can also be broken down by enzymes called ceramidases, leading to the formation of sphingosine, Moreover, a phosphate ...
However, carmofur is a highly potent acid ceramidase (AC) inhibitor. Ceramide influences cancer cell survival, growth and death ... "Acid ceramidase and its inhibitors: a de novo drug target and a new class of drugs for killing glioblastoma cancer stem cells ... "Acid ceramidase is a novel drug target for pediatric brain tumors". Oncotarget. 8 (15): 24753-24761. doi:10.18632/oncotarget. ... "Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity". Scientific Reports. 3 ( ...
Alkaline ceramidase 1 also known as ACER1 is a ceramidase enzyme which in humans is encoded by the ACER1 gene. ACER1 mediates ... "Upregulation of the human alkaline ceramidase 1 and acid ceramidase mediates calcium-induced differentiation of epidermal ... Ito M, Okino N, Tani M, Mitsutake S, Mori K (Mar 2002). "[Molecular evolution of neutral ceramidase: signalling molecule and ... "Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase: an enzyme that preferentially regulates ...
In chronic myelogenous leukemia (CML), IRF8 regulates acid ceramidase to mediate CML apoptosis. IRF8 is highly expressed in ... "IRF8 regulates acid ceramidase expression to mediate apoptosis and suppresses myelogeneous leukemia". Cancer Res. 71 (8): 2882- ...
Finally, ceramide may be broken down by a ceramidase to form sphingosine. Sphingosine may be phosphorylated to form sphingosine ...
Normally, the enzyme ceramidase breaks down fatty material in the body's cells. In Farber disease, the gene responsible for ... Farber disease (also known as Farber's lipogranulomatosis, ceramidase deficiency, "Fibrocytic dysmucopolysaccharidosis," and " ... autosomal recessive lysosomal storage disease marked by a deficiency in the enzyme ceramidase that causes an accumulation of ...
Ceramidase, an enzyme primarily present in plasma membrane, will convert ceramide to sphingosine. sphingosine is then ...
... neutral ceramidase, and sphingolipid metabolites". Pediatr. Res. 61 (1): 61-6. doi:10.1203/01.pdr.0000250534.92934.c2. PMID ...
"A neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens (Stål)". ...
On the other hand, NAAA is homologous to acid ceramidase (HUGO gene symbol: ASAH1), sharing 30% sequence identity at the amino ... NAAA's primary structure is nearly identical to that of acid ceramidase (AC), whose only difference is the substitution of Leu ... While it was discovered within the last decade, its structural similarity to the more familiar acid ceramidase (AC) and ... a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase". The ...
... can be further hydrolyzed by acid ceramidase to form sphingosine and a free fatty acid, both of which are able to ... Anandamide Ceramidase inhibitors Chemotherapeutic agents Fas ligand Endotoxin Homocysteine Heat Gamma interferon Ionizing ...
... and they identified acid ceramidase as the enzyme deficient in Farber's disease. In 1964, Moser returned to the Joseph P. ...
Neutral ceramidase B also known as non-lysosomal ceramidase B or N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2B ... or ASAH2B is a ceramidase enzyme which in humans is encoded by the ASAH2B gene. ASAH2B shows reduced expression with increasing ...
... instability immunodeficiency syndrome Centronuclear myopathy Centrotemporal epilepsy Cephalopolysyndactyly Ceramidase ...
... alkaline ceramidase 1) This disambiguation page lists articles associated with the same title formed as a letter-number ...
Acid Ceramidase inhibitors). Daniele Piomelli is scientific cofounder of Kadmus Pharmaceuticals and Thesan Pharmaceuticals. Di ... "Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity". Science Reports 3:1035. ...
... acid ceramidase), an enzymatic product of ASAH1, gene mutated in Farber disease This disambiguation page lists articles ...
... acid ceramidase) 1 ASPH: encoding enzyme Aspartyl/asparaginyl beta-hydroxylase AZIN1: encoding protein Antizyme inhibitor 1 ...
These are LSDs: Sphingolipidoses Ceramidase Farber disease Krabbe disease Infantile onset Late onset Galactosialidosis ...
It is an inhibitor of the sphingolipid signaling pathway, via specific ceramidase inhibition (ceramidase converts ceramide to ...
... ceramidase EC 3.5.1.24: choloylglycine hydrolase EC 3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase EC 3.5.1.26: N4-(beta ...
Presently, 7 human ceramidases encoded by 7 distinct genes have been cloned: acid ceramidase (ASAH1) - cell survival neutral ... Human neutral ceramidase (nCDase) is an enzyme that plays a critical role in colon cancer and there are currently no potent or ... Ceramidase at the US National Library of Medicine Medical Subject Headings (MeSH) EC 3.5.1.23 Biology portal v t e. ... Ceramidase (EC 3.5.1.23, acylsphingosine deacylase, glycosphingolipid ceramide deacylase) is an enzyme which cleaves fatty ...
Thus, the two substrates of this enzyme are GTP and H2O, whereas its 3 products are formate, 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine, and diphosphate.. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is GTP 7,8-8,9-dihydrolase (diphosphate-forming). Other names in common use include guanosine triphosphate cyclohydrolase II, and GTP-8-formylhydrolase. This enzyme participates in riboflavin metabolism.. ...
Presently, 7 human ceramidases encoded by 7 distinct genes have been cloned: acid ceramidase (ASAH1) - cell survival neutral ... Human neutral ceramidase (nCDase) is an enzyme that plays a critical role in colon cancer and there are currently no potent or ... Ceramidase at the US National Library of Medicine Medical Subject Headings (MeSH) EC 3.5.1.23 Biology portal v t e. ... Ceramidase (EC 3.5.1.23, acylsphingosine deacylase, glycosphingolipid ceramide deacylase) is an enzyme which cleaves fatty ...
The most potent in vitro inhibitor of acid ceramidase, B-13, recently revealed to be virtually inactive towards lysosomal acid ... Potent Inhibition of Acid Ceramidase by Novel B-13 Analogues. Denny Proksch, Jan Jasper Klein, and Christoph Arenz ... Indeed, inhibitors of acid ceramidase have been reported to induce cell death and to display potentiating effects to classical ... Herein, we report a novel B-13-derived scaffold for more effective inhibitors of acid ceramidase. Furthermore, we provide hints ...
Acid ceramidase (AC) is a lysosomal cysteine hydrolase that catalyzes the conversion of ceramide into fatty acid and ... Role of acid ceramidase in resistance to FasL: therapeutic approaches based on acid ceramidase inhibitors and FasL gene therapy ... Complete Acid Ceramidase ablation prevents cancer-initiating cell formation in melanoma cells. *Michele Lai1,2,3,4. , ... Acid ceramidase (N-acylsphingosine deacylase, #EC 3.5.1.23; AC) is a lysosomal cysteine hydrolase encoded by the ASAH1 gene, ...
Compare neutral ceramidase ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, ... Your search returned 9 neutral ceramidase ELISA ELISA Kit across 4 suppliers. ...
Compare Acid Ceramidase ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... Acid Ceramidase ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and ... Your search returned 15 Acid Ceramidase ELISA ELISA Kit across 3 suppliers. ...
... Marie-Elaine ... "Blockade of Lysosomal Acid Ceramidase Induces GluN2B-Dependent Tau Phosphorylation in Rat Hippocampal Slices," Neural ...
Acid ceramidase subunit beta. B, D. 253. Homo sapiens. Mutation(s): 1 Gene Names: ASAH1, ASAH, HSD-33, HSD33. EC: 3.5.1.23 (PDB ... Acid ceramidase subunit alpha. A, C. 131. Homo sapiens. Mutation(s): 2 Gene Names: ASAH1, ASAH, HSD-33, HSD33. EC: 3.5.1.23 ( ... Human acid ceramidase (AC) is a lysosomal cysteine amidase, which has received a great deal of interest in recent years as a ... Human acid ceramidase (AC) is a lysosomal cysteine amidase, which has received a great deal of interest in recent years as a ...
IPR006823 Ceramidase_alk. IPR038445 NCDase_C_sf. IPR031331 NEUT/ALK_ceramidase_C. ... IPR006823 Ceramidase_alk. IPR038445 NCDase_C_sf. IPR031331 NEUT/ALK_ceramidase_C. ... Putative inactive neutral ceramidase BAdd BLAST. 165. Proteomic databases. PaxDb, a database of protein abundance averages ... Belongs to the neutral ceramidase family.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non-supervised ...
Nonlysosomal ceramidase Ceramidase (CDase) is an enzyme that hydrolyzes the N-acyl linkage between a fatty acid and a sphingoid ... Cloning of a ceramidase homologue from Mycobacterium tuberculosis. J Biol Chem. 1999;274:36616-22.CrossRefPubMedGoogle Scholar ... A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem. 2000b;275:11229-34.CrossRefPubMedGoogle ... Tani M., Ito M. (2018) Neutral Ceramidase. In: Choi S. (eds) Encyclopedia of Signaling Molecules. Springer, Cham. * .RIS Papers ...
Markedly perturbed hematopoiesis in acid ceramidase deficient mice. Shaalee Dworski, Alexandra Berger, Caren Furlonger, Joshua ... Markedly perturbed hematopoiesis in acid ceramidase deficient mice. Shaalee Dworski, Alexandra Berger, Caren Furlonger, Joshua ... Markedly perturbed hematopoiesis in acid ceramidase deficient mice Message Subject (Your Name) has forwarded a page to you from ...
Alkaline ceramidase YPC1Add BLAST. 316. Proteomic databases. PaxDb, a database of protein abundance averages across all three ... Alkaline ceramidase YPC1 (EC:3.5.1.-*Search proteins in UniProtKB for this EC number. ... "Characterization of yeast mutants lacking alkaline ceramidases YPC1 and YDC1.". Voynova N.S., Mallela S.K., Vazquez H.M., ... Belongs to the alkaline ceramidase family.Curated. Keywords - Domaini. Transmembrane, Transmembrane helix. Phylogenomic ...
... J Pathol. 2016 ... Here we reveal the essential role of alkaline ceramidase 1 (Acer1) in the skin. Acer1-deficient (Acer1(-/-) ) mice showed ... the levels of which are tightly regulated by a balance of ceramide synthase and ceramidase activities. ...
FD is caused by mutations in the ASAH1 gene, resulting in deficient acid ceramidase (ACDase) activity. Patients with ACDase ... Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment Am J Pathol. 2019 Feb;189(2):320-338. ... FD is caused by mutations in the ASAH1 gene, resulting in deficient acid ceramidase (ACDase) activity. Patients with ACDase ...
The ceramidase activity was in the linear range with time and protein concentration. Of note, the linear range with time was ... Because the observed ceramidase activity is low, amidase activity for other natural lipids cannot be ruled out at this stage. ... In this mechanism, which is similar to what was proposed for neutral ceramidase (ref. 12 in the main text), the zinc ion ... These data strongly suggest that all members of the PAQR family may have a ceramidase activity. (c) Sequence alignment of the ...
Biology the absence of a gene or a region of a chromosome normally present Explanation of Ceramidase deficiency ... Looking for Ceramidase deficiency? Find out information about Ceramidase deficiency. ... redirected from Ceramidase deficiency). Also found in: Dictionary, Thesaurus, Medical, Legal, Financial. deficiency. Biology ... Ceramidase deficiency , Article about Ceramidase deficiency by The Free Dictionary https://encyclopedia2.thefreedictionary.com/ ...
... Duan, Rui-Dong LU ; Verkade, H J; Cheng, ... Alkaline sphingomyelinase (Alk-SMase) and neutral ceramidase (N-CDase) in the intestinal microvillar membrane are responsible ... Alkaline sphingomyelinase (Alk-SMase) and neutral ceramidase (N-CDase) in the intestinal microvillar membrane are responsible ... article{2fea1906-449d-4cca-ac43-8bace27c1bba, abstract = {Alkaline sphingomyelinase (Alk-SMase) and neutral ceramidase (N-CDase ...
Abstract 5484: Targeted inhibition of acid ceramidase and C6-ceramide exposure induces synergistic killing in cancer cells. ... Abstract 5484: Targeted inhibition of acid ceramidase and C6-ceramide exposure induces synergistic killing in cancer cells ... Abstract 5484: Targeted inhibition of acid ceramidase and C6-ceramide exposure induces synergistic killing in cancer cells ... Abstract 5484: Targeted inhibition of acid ceramidase and C6-ceramide exposure induces synergistic killing in cancer cells ...
In ceramide metabolism, ceramidases catalyze the breakdown of ceramide to sphingosine and the inhibition of ceramidase activity ... H9 cells, a human T cell lymphoma cell line, were cultured in medium with the acid ceramidase inhibitor N-oleoyl-ethanolamine ( ... Herein, we report that modulation of ceramide metabolism by extended treatment with a ceramidase inhibitor allows cells to ... extended treatment with an acid ceramidase inhibitor modulates the metabolism of ceramide and selects for a radiation-resistant ...
... ... These cells accumulate ceramide as the result of an inherited deficiency of acidic ceramidase. We demonstrate that Farber ... absence, activation, Apoptosis, caspase-3, cd95, ceramidase, Ceramide, death, Farber disease, fas, gd3 ganglioside, Mice, ...
The synthesis of a novel fluorogenic ceramidase substrate, and its processing both in vitro and in cultured cells in a ... The combinatorial chemistry approach for the discovery of acid ceramidase inhibitors requires the availability of a high- ... Similarly, hydrolysis occurred in intact cultured cells that overexpressed acidic ceramidase. The assay was validated for the ... identification and characterization of acidic ceramidase inhibitors by using several alpha-ketoamide ceramide analogues, whose ...
... 2016. *Author(s): Diamanti ...
Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit-NP_597999.1 (MBS7235867) product datasheet at MyBioSource, ELISA Kits ... ACER1 elisa kit :: Mouse Alkaline ceramidase 1 (ACER1) ELISA Kit. Catalog #. MBS7235867 .mycenter { display: block; margin-left ... alkaline ceramidase 1; alkCDase 1; alkaline CDase 1; acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3; N- ... Belongs to the alkaline ceramidase family.. Protein type: Hydrolase; Lipid Metabolism - sphingolipid; EC 3.5.1.23; Endoplasmic ...
Synonym: Ceramidase Deficiency. What is Farbers Disease ?. Farbers disease describes a group of inherited metabolic disorders ... Farbers disease is caused by a deficiency of the enzyme ceramidase. The disease occurs when both parents carry and pass on the ...
What is mitochondrial ceramidase? Meaning of mitochondrial ceramidase medical term. What does mitochondrial ceramidase mean? ... Looking for online definition of mitochondrial ceramidase in the Medical Dictionary? mitochondrial ceramidase explanation free ... redirected from mitochondrial ceramidase) ASAH2. A gene on chromosome 10q11.23 that encodes an amidohydrolase which hydrolyses ... Mitochondrial ceramidase , definition of mitochondrial ceramidase by Medical dictionary https://medical-dictionary. ...
We identified acid ceramidase (ASAH1), a lysosomal enzyme that cleaves ceramide into sphingosine and fatty acid, as being ... or treated with acid ceramidase inhibitor (ACi), with or without D+Q for 48 h, whereupon cell viability was assessed by direct ...
Learn more about Farbers Lipogranulomatosis or Ceramidase Deficiency, Its Types, Causes, Risk Factors, Symptoms, Treatment, ... The ASAH1 gene is a gene that gives instructions for production of an enzyme known as acid ceramidase. Acid ceramidase is ... Farbers lipogranulomatosis is also known as ceramidase deficiency, Farber disease and acid ceramidase deficiency. ... Farbers Lipogranulomatosis or Ceramidase Deficiency: Types, Causes, Symptoms, Treatment. Reviewed By:. Pramod Kerkar, M.D., ...
ceramidase cer·am·i·dase (sə-rāmĭ-dās, -dāz) n. An enzyme that catalyzes the breakdown of ceramides into sphingosine and ... Definithing > Dictionary > c > Ceramidase. Ceramidase. ceramidase cer·am·i·dase (sə-rāmĭ-dās, -dāz). n.. An enzyme that ... Disclaimer: Ceramidase definition / meaning should not be considered complete, up to date, and is not intended to be used in ...
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Our data reveal the existence of a ceramidase (EC 3.5.1.23) in rat intestinal mucosa with an optimal pH of 7.0. One milligram ... Distribution and properties of neutral ceramidase activity in rat intestinal tract. Research output: Contribution to journal › ... The addition of Triton X-100 enhances the ceramidase activity at optimal bile salt concentration. The reaction is linear with ... In conclusion, intestinal neutral ceramidase has a distinct distribution pattern and bile salt dependence, which enables it to ...
... ceramidase producing strain belonging to the genus Nocardia in a culture medium and collecting ganglioside ceramidase from the ... This invention provides a novel ganglioside ceramidase which has a substrate specificity at least for GD1a, GM1, GM2 and GM3 ... to lysoganglioside and fatty acid.This invention further provides a process for producing the novel ganglioside ceramidase ... 4. The ganglioside ceramidase of claim 1, wherein said ganglioside ceramidase is isolated from a bacteria of the genus Nocardia ...
  • Presently, 7 human ceramidases encoded by 7 distinct genes have been cloned: acid ceramidase (ASAH1) - cell survival neutral ceramidase (ASAH2, ASAH2B, ASAH2C) - protective against inflammatory cytokines alkaline ceramidase 1 (ACER1) - mediating cell differentiation by controlling the generation of SPH and S1P alkaline ceramidase 2 (ACER2) - important for cell proliferation and survival alkaline ceramidase 3 (ACER3) A deficiency in ASAH1 is associated with Farber disease. (wikipedia.org)
  • A novel but highly conserved gene family of neutral/alkaline ceramidases. (springer.com)
  • Cloning of an alkaline ceramidase from Saccharomyces cerevisiae. (uniprot.org)
  • Alkaline sphingomyelinase (Alk-SMase) and neutral ceramidase (N-CDase) in the intestinal microvillar membrane are responsible for dietary sphingomyelin digestion. (lu.se)
  • MBS7235867 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline ceramidase 1 (ACER1) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Our long-term goal is to define the mechanisms by which anti-cancer chemotherapeutic agents (ACCAs) induce anti-proliferative responses (proliferation inhibition and programmed cell death) in tumor cells by focusing on human alkaline ceramidase 2 (ACER2) and its lipid substrate (ceramide) and product (sphingosine). (grantome.com)
  • In contrast, our preliminary studies show that increasing the conversion of ceramide to SPH by the Golgi alkaline ceramidase 2 (ACER2) markedly enhances the cytotoxicity of doxorubicin, a known ceramide-inducing ACCA, in HeLa cells, whereas knocking down ACER2 has the opposite effect. (grantome.com)
  • The PI's group has identified a human enzyme called alkaline ceramidase 2 (ACER2) responsible for the generation of SPH from ceramide in tumor cells. (grantome.com)
  • Acer2 (alkaline ceramidase 2) regulates the hydrolysis of ceramides. (thefreedictionary.com)
  • Alkaline ceramidase 2 is the one ceramidase that has been found essential for the generation of normal levels of sphingosine in the blood. (jax.org)
  • Alkaline ceramidase 2 is essential for the homeostasis of plasma sphingoid bases and their phosphates. (jax.org)
  • Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase: an enzyme that preferentially regulates metabolism of very long chain ceramides. (harvard.edu)
  • Cloning and characterization of a novel human alkaline ceramidase. (harvard.edu)
  • Correction: Alkaline Ceramidase 3 Deficiency Results in Purkinje Cell Degeneration and Cerebellar Ataxia Due to Dyshomeostasis of Sphingolipids in the Brain. (nih.gov)
  • FD is caused by mutations in the ASAH1 gene, resulting in deficient acid ceramidase (ACDase) activity. (nih.gov)
  • The ASAH1 gene is a gene that gives instructions for production of an enzyme known as acid ceramidase. (epainassist.com)
  • ASAH1 gene mutations result in storing the functional acid ceramidase that stops the lysosomes from appropriate breaking down of ceramides. (epainassist.com)
  • The ASAH1 gene provides instructions for making an enzyme called acid ceramidase. (medlineplus.gov)
  • Mutations in the ASAH1 gene lead to severe reduction in acid ceramidase, typically to below 10 percent of normal. (medlineplus.gov)
  • Alves MQ, Le Trionnaire E, Ribeiro I, Carpentier S, Harzer K, Levade T, Ribeiro MG. Molecular basis of acid ceramidase deficiency in a neonatal form of Farber disease: identification of the first large deletion in ASAH1 gene. (medlineplus.gov)
  • We tested single nucleotide polymorphisms (SNPs) in the acid ceramidase gene ( ASAH1 ) for association with subject adherence and physiologic benefit with exercise training in two well-characterised randomised, controlled 8-month exercise interventions: STRRIDE I ( n = 239) and STRRIDE II ( n = 246). (gwu.edu)
  • This study demonstrates that GBMs acquire resistance to radiation via upregulation of acid ceramidase (ASAH1) and sphingosine‑1-phosphate (Sph-1P). (mcw.edu)
  • The ASAH1 Gene codes for acid ceramidase which is an enzyme found in lysosomes. (wikipedia.org)
  • Human neutral ceramidase (nCDase) is an enzyme that plays a critical role in colon cancer and there are currently no potent or clinically effective inhibitors for nCDase reported to date. (wikipedia.org)
  • Your search returned 9 neutral ceramidase ELISA ELISA Kit across 4 suppliers. (biocompare.com)
  • Structural basis for ceramide recognition and hydrolysis by human neutral ceramidase. (springer.com)
  • Role of neutral ceramidase in colon cancer. (springer.com)
  • Ito M, Okino N, Tani M. New insight into the structure, reaction mechanism, and biological functions of neutral ceramidase. (springer.com)
  • Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids. (springer.com)
  • Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury. (springer.com)
  • Okino N, Mori K, Ito M. Genomic structure and promoter analysis of the mouse neutral ceramidase gene. (springer.com)
  • Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease. (springer.com)
  • Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death. (springer.com)
  • Tani M, Okino N, Mitsutake S, Tanigawa T, Izu H, Ito M. Purification and characterization of a neutral ceramidase from mouse liver. (springer.com)
  • Tani M, Okino N, Mori K, Tanigawa T, Izu H, Ito M. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. (springer.com)
  • Home Research Outputs Distribution and properties of neutral ceramidase activity i. (lu.se)
  • In conclusion, intestinal neutral ceramidase has a distinct distribution pattern and bile salt dependence, which enables it to collaborate with intestinal sphingomyelinase in hydrolysis of sphingomyelin. (lu.se)
  • Increasing expression of neutral ceramidase ASAH2 on the plasma membrane also attenuates cell apoptosis induced by TNF-1, a known ceramide-generating cytokine. (grantome.com)
  • El Bawab, "Bimodal effect of advanced glycation end products on mesangial cell proliferation is mediated by neutral ceramidase regulation and endogenous sphingolipids," The Journal of Biological Chemistry, vol. (thefreedictionary.com)
  • In this study we report the purification and characterization of a novel second type of neutral ceramidase from rat brain (RBCDase II). (nyu.edu)
  • SIMILARITY: Belongs to the neutral ceramidase family. (univ-lyon1.fr)
  • Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10781606, PubMed:16229686, PubMed:26190575). (genecards.org)
  • Journal Article] Molecular cloning and functional analysis of zebrafish neutral ceramidase. (nii.ac.jp)
  • Journal Article] Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization, and enzyme activity of neutral ceramidase. (nii.ac.jp)
  • O-glycosylation of mucin-like domain retains the neutral ceramidase on the plasma membranes as a type II integral membrane protein'Journal of Biological Chemistry. (nii.ac.jp)
  • Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1. (nii.ac.jp)
  • A neutral ceramidase homologue from Dictyosterium discoideum exhibits an acidic pH optimum'Biochemical Journal. (nii.ac.jp)
  • Genomic structure and promoter analysis of the mouse neutral ceramidase gene. (nii.ac.jp)
  • Both acidic ceramidase (aCDase) and neutral ceramidase (nCDase) activities declined after L- and H-UVB, but returned to normal only in L-UVB cells, with decreased CDase activities or mRNA or protein levels being sustained in H-UVB cells. (biomedsearch.com)
  • Purification and biochemical characterization of membrane-bound neutral ceramidase from camel brain (Camelus dromedarius). (harvard.edu)
  • Farber's lipogranulomatosis is also known as ceramidase deficiency, Farber disease and acid ceramidase deficiency. (epainassist.com)
  • In conjunction, RVT-801 is a recombinant form of human acid ceramidase (rhAC) being developed as a potential enzyme replacement therapy for acid ceramidase deficiency, manifesting as Farber disease. (thefreedictionary.com)
  • Enzyvant, a subsidiary of Roivant Sciences, has selected Avid Bioservices as the commercial drug substance manufacturer for RVT801, its recombinant human acid ceramidase enzyme replacement therapy being developed as a potential treatment for Farber disease. (thefreedictionary.com)
  • Bär J, Linke T, Ferlinz K, Neumann U, Schuchman EH, Sandhoff K. Molecular analysis of acid ceramidase deficiency in patients with Farber disease. (medlineplus.gov)
  • Farber disease is a rare autosomal recessive disorder caused by acid ceramidase deficiency that usually presents as early-onset progressive visceral and neurologic disease. (elsevier.com)
  • The ceramidase ACER2 hydrolyzes very long chain ceramides to generate;sphingosine (Xu et al. (creative-biogene.com)
  • Ceramidase (CDase) is an enzyme that hydrolyzes the N -acyl linkage between a fatty acid and a sphingoid base in ceramide (Cer) and is widely distributed from bacteria to mammals. (springer.com)
  • Ceramidases (CDase) are enzymes that catalyze the hydrolysis of N-acyl linkage of ceramide (Cer) to generate sphingosine and free fatty acids. (nyu.edu)
  • 3) A salvage pathway generates ceramides by recycling sphingosine via CerS, as the sphingosine is produced by the hydrolysis of ceramide catalyzed by ceramidase (CDase) [ 4 ]. (clinsci.org)
  • A research group that previously showed that overexpression of ceramidase (CDase) in Drosophila photoreceptors suppressed retinal degeneration in response to certain mutations has now made the intriguing discovery that CDase regulation of photoreceptor function is cell nonautonomous. (sciencemag.org)
  • Inhibition of the lysosomal acid ceramidase can increase cellular ceramide levels and thus induce apoptosis. (hindawi.com)
  • In ceramide metabolism, ceramidases catalyze the breakdown of ceramide to sphingosine and the inhibition of ceramidase activity results in the intracellular accumulation of ceramide leading to apoptosis. (aacrjournals.org)
  • Herein, we report that modulation of ceramide metabolism by extended treatment with a ceramidase inhibitor allows cells to acquire resistance to radiation-induced apoptosis in a radiosensitive human T cell lymphoma cell line. (aacrjournals.org)
  • In an in vitro study, overexpression of acid ceramidase protected murine fibroblasts from TNF[alpha]induced cell apoptosis by shifting elevated ceramide towards cell survival sphingosine-1-phosphate [31]. (thefreedictionary.com)
  • Liu, IRF8 Regulates Acid Ceramidase Expression to Mediate Apoptosis and Suppresses Myelogeneous Leukemia, J. (thefreedictionary.com)
  • Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. (genecards.org)
  • Acid ceramidase is present in the compartments, which digest and recycle materials in the cell by breaking down the fats known as ceramides for the purpose of getting them used by the body. (epainassist.com)
  • Lack of activity of acid ceramidase can develop ceramides in the lysosomes of tissues and cells in the colon, muscles used for movement, bone, cartilage, lung and liver. (epainassist.com)
  • Acid ceramidase breaks down fats called ceramides into a fat called sphingosine and a fatty acid. (medlineplus.gov)
  • The acid ceramidase enzyme is required to help break down certain fatty substances - called ceramides - in the body. (rarediseases.org)
  • Ceramidases (EC 3.5.1.23) are a group of enzymes which catalyze the hydrolysis of ceramides to produce sphingosine, which subsequently undergoes phosphorylation to generate sphingosine-1-phosphate (S1P). (genecards.org)
  • Ceramidase (EC 3.5.1.23, acylsphingosine deacylase, glycosphingolipid ceramide deacylase) is an enzyme which cleaves fatty acids from ceramide, producing sphingosine (SPH) which in turn is phosphorylated by a sphingosine kinase to form sphingosine-1-phosphate (S1P). (wikipedia.org)
  • Our data reveal the existence of a ceramidase (EC 3.5.1.23) in rat intestinal mucosa with an optimal pH of 7.0. (lu.se)
  • Indeed, inhibitors of acid ceramidase have been reported to induce cell death and to display potentiating effects to classical radio- or chemo therapy in a number of in vitro and in vivo cancer models. (hindawi.com)
  • Herein, we report a novel B-13-derived scaffold for more effective inhibitors of acid ceramidase. (hindawi.com)
  • Several investigations have shown that acid ceramidase inhibitors are potential antiproliferative and cytostatic drugs for cancer chemotherapy. (inserm.fr)
  • The combinatorial chemistry approach for the discovery of acid ceramidase inhibitors requires the availability of a high-throughput enzyme assay. (inserm.fr)
  • The assay was validated for the identification and characterization of acidic ceramidase inhibitors by using several alpha-ketoamide ceramide analogues, whose inhibitory activity had been previously described. (inserm.fr)
  • Previous in Vitro study has found that acid ceramidase inhibitors (e.g. carmofur) have better efficacy than the standard chemotherapeutic drug available. (uwm.edu)
  • This study aims to identify the role of acid ceramidase in glioblastoma and the efficacy of acid ceramidase inhibitors in suppressing the tumor, which may potentially lead to the development of a new chemotherapeutic agent for treating glioblastoma. (uwm.edu)
  • This model system will be used to study if acid ceramidase inhibitors can cross the blood-brain barrier, and their role in tumor suppression using imaging microscopy and mass spectrometric techniques. (uwm.edu)
  • Alterations in this gene result in a deficiency of the enzyme acid ceramidase. (rarediseases.org)
  • Here, we describe a micelleplex system based on an amphiphilic and cationic triblock copolymer, which can systemically deliver siRNA targeting the acid ceramidase (AC) gene for cancer therapy. (naver.com)
  • Biochemical characterization of the reverse activity of rat brain ceramidase. (springer.com)
  • Our results suggest a new target for Tau dephosphorylation and provide an insight into the potential therapeutic effects of brain ceramidase in Alzheimer's disease and other Tau-related pathologies.Acknowledgements - This work was supported by the Canadian Research Chair in Molecular Neuropharmacology (Michel Cyr) and by the National Sciences and Engineering Council of Canada (Guy Massicotte). (neurologycongress.com)
  • The most potent in vitro inhibitor of acid ceramidase, B-13, recently revealed to be virtually inactive towards lysosomal acid ceramidase in living cells. (hindawi.com)
  • In this study, we evaluated the anticancer activity of DM102 [(2R,3Z)-N-(1-hydroxyoctadec-3-en-2-yl)pivalamide], a novel inhibitor of the enzyme acid ceramidase (AC), a key regulator of ceramide metabolism, in combination with C6-ceramide (C6-cer) in human breast cancer cells. (aacrjournals.org)
  • H9 cells, a human T cell lymphoma cell line, were cultured in medium with the acid ceramidase inhibitor N-oleoyl-ethanolamine (NOE), starting at a concentration level of 10 μM, with gradual increases to an NOE concentration of 100 μM. (aacrjournals.org)
  • These results suggest that the absence of intracellular accumulation of ceramide following irradiation, through the modulation of ceramide metabolism using an acid ceramidase inhibitor for extended periods of time in culture, represents one potential mechanism for the development of radioresistance. (aacrjournals.org)
  • Methods and Results - Transverse rat hippocampal slices were prepared with a McIlwain tissue chopper, placed on a nylon mesh in a liquid-gas interface chamber and treated for a period of 3 hours with an acid ceramidase inhibitor (ACI). (neurologycongress.com)
  • Furthermore, we provide hints for an introduction of basic functional groups at an alternative site of the B-13 scaffold that do not interfere with acid ceramidase inhibition in vitro . (hindawi.com)
  • In addition, inhibition of acid ceramidase with ceranib-2 has been reported to lead to apoptotic cell death in 5RP7 cells (Vejselova et al. (thefreedictionary.com)
  • Acid ceramidase upregulation in prostate cancer cells confers resistance to radiation: AC inhibition, a potential radiosensitizer," Molecular Therapy, vol. (thefreedictionary.com)
  • Adiponectin receptors (ADIPORs) are integral membrane proteins controlling glucose and lipid metabolism by mediating, at least in part, a cellular ceramidase activity 1 which catalyzes the hydrolysis of ceramide to produce sphingosine and a free fatty acid (FFA). (pubmedcentralcanada.ca)
  • Ceramide, a key molecule in the metabolism of sphingolipids, is converted by ceramidase to sphingosine, and phosphorylated by ceramide kinase to form ceramide-1-phosphate (C1P). (chat-shuffle.net)
  • These represent the first inducible genetic models that acutely manipulate adiponectin receptor signaling in adult mouse tissues, which allows us to directly assess AdipoR signaling on glucose and lipid metabolism.Overexpression of either adiponectin receptor isoform in the adipocyte or hepatocyte is sufficient to enhance ceramidase activity, whole body glucose metabolism, and hepatic insulin sensitivity, while opposing hepatic steatosis. (hepaton.com)
  • When challenged with a leptin-deficient genetic model of type 2 diabetes, AdipoR2 expression in adipose or liver is sufficient to reverse hyperglycemia and glucose intolerance.These observations reveal that adiponectin is critical for AdipoR-induced ceramidase activation which enhances hepatic glucose and lipid metabolism via rapidly acting 'cross-talk' between liver and adipose tissue sphingolipids. (hepaton.com)
  • Zebrafish acid ceramidase: Expression in Pichia pastoris GS115and biochemical characterization. (annals.org)
  • In contrast, a number of weakly basic B-13 analogues have been shown to accumulate in the acidic compartments of living cells and to efficiently inhibit lysosomal acid ceramidase. (hindawi.com)
  • These cells accumulate ceramide as the result of an inherited deficiency of acidic ceramidase. (diva-portal.org)
  • Similarly, hydrolysis occurred in intact cultured cells that overexpressed acidic ceramidase. (inserm.fr)
  • Inducible overexpression of adiponectin receptors highlight the roles of adiponectin-induced ceramidase signaling in lipid and glucose homeostasis. (hepaton.com)
  • Guy Massicotte and his colleagues will propose that ceramidase overexpression may eventually protect the human brain against Alzheimer's disease pathology by slowing-down Tau hyperphosphorylation, secretion and aggregation. (neurologycongress.com)
  • Ceramide can be hydrolyzed into sphingosine by ceramidases in human cells and, like ceramide, SPH can potently induce anti-proliferative effects in tumor cells both in vitro and in animal models. (grantome.com)
  • This week's highlighted article shows that adiponectin receptors may transduce signaling by acting as ceramidases. (sciencemag.org)
  • Here, we show that expression of ceramidase facilitates the dissolution of incompletely formed and inappropriately located elements of rhabdomeric membranes in ninaE(I17) mutants lacking the G protein receptor Rh1 in R1-R6 photoreceptor cells. (umassmed.edu)
  • Acid ceramidase (AC) is a lysosomal cysteine hydrolase that catalyzes the conversion of ceramide into fatty acid and sphingosine. (nature.com)
  • Human acid ceramidase (AC) is a lysosomal cysteine amidase, which has received a great deal of interest in recent years as a potential target for the development of new therapeutics against melanoma and glioblastoma tumors. (rcsb.org)
  • NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (1). (novusbio.com)
  • NAAA is a member of the choloylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (1). (novusbio.com)
  • The addition of Triton X-100 enhances the ceramidase activity at optimal bile salt concentration. (lu.se)
  • Acid Ceramidase Treatment Enhances the Outcome of Autologous Chondrocyte Implantation in a Rat Osteochondral Defect Model. (exponent.com)
  • Ceramidase expression facilitates membrane turnover and endocytosis of" by Usha Acharya, Michael Beth Mowen et al. (umassmed.edu)
  • Transgenic expression of ceramidase suppresses retinal degeneration in Drosophila arrestin and phospholipase C mutants. (umassmed.edu)
  • Ceramidase expression facilitates the endocytic turnover of Rh1. (umassmed.edu)
  • Although ceramidase expression aids the removal of internalized rhodopsin, it does not affect the turnover of Rh1 in photoreceptors maintained in dark, where Rh1 is not activated and thus has a slower turnover and a long half-life. (umassmed.edu)
  • Therefore, the phenotypic consequence of ceramidase expression in photoreceptors is caused by facilitation of endocytosis. (umassmed.edu)
  • Also included are a number of the catabolic genes, e.g., sphingomyelinases, SMases, ceramidases, ASAH, sphingosine kinases, for the formation of sphinganine 1-phosphate (Sa1P) and sphingosine 1-phosphate (So1P), and phosphatases for the reverse reaction and the lyase that cleaves sphingoid base 1-phosphates to ethanolamine phosphate (EP), hexadecanal (C16:0al) and hexadecenal (C16:1al). (wikipathways.org)
  • Cloning of a ceramidase homologue from Mycobacterium tuberculosis. (springer.com)
  • The ceramidase activity is low, however, and further studies will be required to fully characterize ADIPORs enzymatic parameters and their substrate specificity. (pubmedcentralcanada.ca)
  • More particularly, it relates to a ganglioside ceramidase having physico-chemical properties shown at least by the following substrate specificity and enzyme action. (justia.com)
  • Effects of bile diversion in rats on intestinal sphingomyelinases and ceramidase. (lu.se)
  • UWM Undergraduate Research Symposium: Role of Acid Ceramidase In Xenografted Zebrafish Glioblastoma Model. (uwm.edu)
  • The reverse activity of human acid ceramidase. (nii.ac.jp)
  • Here, we determined crystal structures of ADIPOR2 bound to a FFA molecule and demonstrate that ADIPOR2 possesses an intrinsic basal ceramidase activity enhanced by adiponectin. (pubmedcentralcanada.ca)
  • Because we found that ADIPOR1 also possesses an intrinsic ceramidase activity, we suspect that the two distinct structures may represent key steps of the ADIPORs enzymatic activity. (pubmedcentralcanada.ca)
  • Recently, the pleiotropic actions of adiponectin were linked to the ceramide signaling pathway as ADIPOR1 and ADIPOR2 activation was shown to lower ceramide levels via the activation of a ceramidase activity measured in crude cell lysates 1 . (pubmedcentralcanada.ca)
  • Finally, the activity shows a considerable resistance against tryptic degradation, as 71% of the ceramidase activity remained when the homogenates were preincubated with high concentrations of trypsin. (lu.se)
  • Receptor-mediated activation of ceramidase activity initiates the pleiotropic actions of adiponectin," Nature Medicine, vol. (thefreedictionary.com)
  • It is unclear whether the level of acid ceramidase activity is related to the severity of the disorder. (medlineplus.gov)