A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance.
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.
A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.
The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. It mediates proper CHROMOSOME SEGREGATION and contractile ring function during CYTOKINESIS.
A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. They are involved in many aspects of cell division, including centrosome duplication, SPINDLE APPARATUS formation, chromosome alignment, attachment to the spindle, checkpoint activation, and CYTOKINESIS.
Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.
The three-part structure of ribbon-like proteinaceous material that serves to align and join the paired homologous CHROMOSOMES. It is formed during the ZYGOTENE STAGE of the first meiotic division. It is a prerequisite for CROSSING OVER.
The prophase of the first division of MEIOSIS (in which homologous CHROMOSOME SEGREGATION occurs). It is divided into five stages: leptonema, zygonema, PACHYNEMA, diplonema, and diakinesis.
The alignment of CHROMOSOMES at homologous sequences.
A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.
The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Agents that affect MITOSIS of CELLS.
A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.
Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Proteins secreted by the epididymal epithelium. These proteins are both tissue- and species-specific. They are important molecular agents in the process of sperm maturation.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
Carbohydrate antigen most commonly seen in tumors of the ovary and occasionally seen in breast, kidney, and gastrointestinal tract tumors and normal tissue. CA 125 is clearly tumor-associated but not tumor-specific.
Neoplasms composed of glandular tissue, an aggregation of epithelial cells that elaborate secretions, and of any type of epithelium itself. The concept does not refer to neoplasms located in the various glands or in epithelial tissue.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.

Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. (1/99)

Human centromeres contain large arrays of alpha-satellite DNA that are thought to provide centromere function. The arrays show size and sequence variation, but the extent to which extremely low levels of this DNA can occur on normal centromeres is unclear. Using a set of chromosome-specific alpha-satellite probes for each of the human chromosomes, we performed interphase fluorescence in situ hybridization (FISH) in a population-screening study. Our results demonstrate that extreme reduction of chromosome-specific alpha satellite is unusually common in chromosome 21 (screened with the alphaRI probe), with a prevalence of 3.70%, compared to < or =0.12% for each of chromosomes 13 and 17, and 0% for the other chromosomes. No analphoid centromere was identified in >17,000 morphologically normal chromosomes studied. All of the low-alphoid centromeres are fully functional as indicated by their mitotic stability and binding to centromere proteins CENP-B, CENP-C, and CENP-E. Sensitive metaphase FISH analysis of the low-alphoid chromosome 21 centromeres established the presence of residual alphaRI as well as other non-alphaRI alpha-satellite DNA suggesting that centromere function may be provided by (1) the residual alphaRI DNA, (2) other non-alphaRI alpha-satellite sequences, (3) a combination of 1 and 2, or (4) an activated neocentromere DNA. The low-alphoid centromeres, in particular those of chromosome 21, should provide unique opportunities for the study of the evolution and the minimal DNA requirement of the human centromere.  (+info)

Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. (2/99)

Centromere protein B (CENP-B) binds constitutively to mammalian centromere repeat DNA and is highly conserved between humans and mouse. Cenpb null mice appear normal but have lower body and testis weights. We demonstrate here that testis-weight reduction is seen in male null mice generated on three different genetic backgrounds (denoted R1, W9.5, and C57), whereas body-weight reduction is dependent on the genetic background as well as the gender of the animals. In addition, Cenpb null females show 31%, 33%, and 44% reduced uterine weights on the R1, W9.5, and C57 backgrounds, respectively. Production of "revertant" mice lacking the targeted frameshift mutation but not the other components of the targeting construct corrected these differences, indicating that the observed phenotype is attributable to Cenpb gene disruption rather than a neighbouring gene effect induced by the targeting construct. The R1 and W9.5 Cenpb null females are reproductively competent but show age-dependent reproductive deterioration leading to a complete breakdown at or before 9 months of age. Reproductive dysfunction is much more severe in the C57 background as Cenpb null females are totally incompetent or are capable of producing no more than one litter. These results implicate a further genetic modifier effect on female reproductive performance. Histology of the uterus reveals normal myometrium and endometrium but grossly disrupted luminal and glandular epithelium. Tissue in situ hybridization demonstrates high Cenpb expression in the uterine epithelium of wild-type animals. This study details the first significant phenotype of Cenpb gene disruption and suggests an important role of Cenpb in uterine morphogenesis and function that may have direct implications for human reproductive pathology.  (+info)

Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. (3/99)

Centromere protein A (Cenpa for mouse, CENP-A for other species) is a histone H3-like protein that is thought to be involved in the nucleosomal packaging of centromeric DNA. Using gene targeting, we have disrupted the mouse Cenpa gene and demonstrated that the gene is essential. Heterozygous mice are healthy and fertile whereas null mutants fail to survive beyond 6.5 days postconception. Affected embryos show severe mitotic problems, including micronuclei and macronuclei formation, nuclear bridging and blebbing, and chromatin fragmentation and hypercondensation. Immunofluorescence analysis of interphase cells at day 5.5 reveals complete Cenpa depletion, diffuse Cenpb foci, absence of discrete Cenpc signal on centromeres, and dispersion of Cenpb and Cenpc throughout the nucleus. These results suggest that Cenpa is essential for kinetochore targeting of Cenpc and plays an early role in organizing centromeric chromatin at interphase. The evidence is consistent with the proposal of a critical epigenetic function for CENP-A in marking a chromosomal region for centromere formation.  (+info)

Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. (4/99)

Two functionally important DNA sequence elements in centromeres of the fission yeast Schizosaccharomyces pombe are the centromeric central core and the K-type repeat. Both of these DNA elements show internal functional redundancy that is not correlated with a conserved DNA sequence. Specific, but degenerate, sequences in these elements are bound in vitro by the S. pombe DNA-binding proteins Abp1p (also called Cbp1p) and Cbhp, which are related to the mammalian centromere DNA-binding protein CENP-B. In this study, we determined that Abp1p binds to at least one of its target sequences within S. pombe centromere II central core (cc2) DNA with an affinity (K(s) = 7 x 10(9) M(-1)) higher than those of other known centromere DNA-binding proteins for their cognate targets. In vivo, epitope-tagged Cbhp associated with centromeric K repeat chromatin, as well as with noncentromeric regions. Like abp1(+)/cbp1(+), we found that cbh(+) is not essential in fission yeast, but a strain carrying deletions of both genes (Deltaabp1 Deltacbh) is extremely compromised in growth rate and morphology and missegregates chromosomes at very high frequency. The synergism between the two null mutations suggests that these proteins perform redundant functions in S. pombe chromosome segregation. In vitro assays with cell extracts with these proteins depleted allowed the specific assignments of several binding sites for them within cc2 and the K-type repeat. Redundancy observed at the centromere DNA level appears to be reflected at the protein level, as no single member of the CENP-B-related protein family is essential for proper chromosome segregation in fission yeast. The relevance of these findings to mammalian centromeres is discussed.  (+info)

Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (5/99)

BACKGROUND: Survivin is a mammalian protein that carries a motif typical of the inhibitor of apoptosis (IAP)proteins, first identified in baculoviruses. Although baculoviral IAP proteins regulate cell death, the yeast Survivin homolog Bir1 is involved in cell division. To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice. RESULTS: In human cells, Survivin appeared first on centromeres bound to a novel para-polar axis during prophase/metaphase, relocated to the spindle midzone during anaphase/telophase, and disappeared at the end of telophase. In the mouse, Survivin was required for mitosis during development. Null embryos showed disrupted microtubule formation, became polyploid, and failed to survive beyond 4.5days post coitum. This phenotype, and the cell-cycle localization of Survivin, resembled closely those of INCENP. Because the yeast homolog of INCENP, Sli15, regulates the Aurora kinase homolog Ipl1p, and the yeast Survivin homolog Bir1 binds to Ndc10p, a substrate of Ipl1p, yeast Survivin, INCENP and Aurora homologs function in concert during cell division. CONCLUSIONS: In vertebrates, Survivin and INCENP have related roles in mitosis, coordinating events such as microtubule organization, cleavage-furrow formation and cytokinesis. Like their yeast homologs Bir1 and Sli15, they may also act together with the Aurora kinase.  (+info)

Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. (6/99)

Three human centromere proteins, CENP-A, CENP-B and CENP-C, are a set of autoantigens specifically recognized by anticentromere antibodies often produced by patients with scleroderma. Microscopic observation has indicated that CENP-A and CENP-C localize to the inner plate of metaphase kinetochore, while CENP-B localizes to the centromere heterochromatin beneath the kinetochore. The antigenic structure, called "prekinetochore", is also present in interphase nuclei, but little is known about its molecular organization and the relative position of these antigens. Here, to visualize prekinetochore in living cells, we first obtained a stable human cell line, MDA-AF8-A2, in which human CENP-A is exogenously expressed as a fusion to a green fluorescent protein of Aequorea victoria. Simultaneous staining with anti-CENP-B and anti-CENP-C antibodies showed that the recombinant CENP-A colocalized with the endogenous CENP-C and constituted small discrete dots attaching to larger amorphous mass of CENP-B heterochromatin. When the cell growth was arrested in G1/ S phase with hydroxyurea, CENP-B heterochromatin was sometimes highly extended, while the relative location between GFP-fused CENP-A and the endogenous CENP-C was not affected. These results indicated that the fluorescent CENP-A faithfully localizes to the centromere/kinetochore throughout the cell cycle. We then obtained several mammalian cell lines where the same GFP-fused human CENP-A construct was stably expressed and their centromere/kinetochore is fluorescent throughout the cell cycle. These cell lines will further be used for visualizing the prekinetochore locus in interphase nuclei as well as analyzing kinetochore dynamics in the living cells.  (+info)

Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. (7/99)

Several members of protein families that are conserved in higher eukaryotes are known to play a role in centromere function in the fission yeast Schizosaccharomyces pombe, including two homologs of the mammalian centromere protein CENP-B, Abp1p and Cbh1p. Here we characterize a third S. pombe CENP-B homolog, Cbh2p (CENP-B homolog 2). cbh2Delta strains exhibited a modest elevation in minichromosome loss, similar to cbh1Delta or abp1Delta strains. cbh2Delta cbh1Delta strains showed little difference in growth or minichromosome loss rate when compared to single deletion strains. In contrast, cbh2Delta abp1Delta strains displayed dramatic morphological and chromosome segregation defects, as well as enhancement of the slow-growth phenotype of abp1Delta strains, indicating partial functional redundancy between these proteins. Both cbh2Delta abp1Delta and cbh1Delta abp1Delta strains also showed strongly enhanced sensitivity to a microtubule-destabilizing drug, consistent with a mitotic function for these proteins. Cbh2p was localized to the central core and core-associated repeat regions of centromeric heterochromatin, but not at several other centromeric and arm locations tested. Thus, like its mammalian counterpart, Cbh2p appeared to be localized exclusively to a portion of centromeric heterochromatin. In contrast, Abp1p was detected in both centromeric heterochromatin and in chromatin at two of three replication origins tested. Cbh2p and Abp1p homodimerized in the budding yeast two-hybrid assay, but did not interact with each other. These results suggest that indirect cooperation between different CENP-B-like DNA binding proteins with partially overlapping chromatin distributions helps to establish a functional centromere.  (+info)

Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. (8/99)

The mechanisms that specify precisely where mammalian kinetochores form within arrays of centromeric heterochromatin remain largely unknown. Localization of CENP-A exclusively beneath kinetochore plates suggests that this distinctive histone might direct kinetochore formation by altering the structure of heterochromatin within a sub-region of the centromere. To test this hypothesis, we experimentally mistargeted CENP-A to non-centromeric regions of chromatin and determined whether other centromere-kinetochore components were recruited. CENP-A-containing non-centromeric chromatin assembles a subset of centromere-kinetochore components, including CENP-C, hSMC1, and HZwint-1 by a mechanism that requires the unique CENP-A N-terminal tail. The sequence-specific DNA-binding protein CENP-B and the microtubule-associated proteins CENP-E and HZW10 were not recruited, and neocentromeric activity was not detected. Experimental mistargeting of CENP-A to inactive centromeres or to acentric double-minute chromosomes was also not sufficient to assemble complete kinetochore activity. The recruitment of centromere-kinetochore proteins to chromatin appears to be a unique function of CENP-A, as the mistargeting of other components was not sufficient for assembly of the same complex. Our results indicate at least two distinct steps in kinetochore assembly: (1) precise targeting of CENP-A, which is sufficient to assemble components of a centromere-prekinetochore scaffold; and (2) targeting of kinetochore microtubule-associated proteins by an additional mechanism present only at active centromeres.  (+info)

Fingerprint Dive into the research topics of A helix-turn-helix structure unit in human centromere protein B (CENP-B). Together they form a unique fingerprint. ...
We report the interaction between a human centromere antigen and an alphoid DNA, a human centromeric satellite DNA, which consists of 170-bp repeating units. A cloned alphoid DNA fragment incubated with a HeLa cell nuclear extract is selectively immunoprecipitated by the anticentromere sera from scleroderma patients. Immunoprecipitation of the DNA made by primer extension defines the 17-bp segment on the alphoid DNA that is required for formation of DNA-antigen complex. On the other hand, when proteins bound to the biotinylated alphoid DNA carrying the 17-bp motif are recovered by streptavidin agarose and immunoblotted, the 80-kD centromere antigen (CENP-B) is detected. DNA binding experiments for proteins immunoprecipitated with anticentromere serum, separated by gel electrophoresis, and transferred to a membrane strongly suggest that the 80-kD antigen specifically binds to the DNA fragment with the 17-bp motif. The 17-bp motif is termed the CENP-B box. Alphoid monomers with the CENP-B box ...
The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box), which appears in every other alpha-satellite repeat. In the present study, the crystal structure of the complex of the DNA-binding region (129 residues) of CENP-B and the CENP-B box DNA has been determined at 2.5 A resolution. The DNA-binding region forms two helix-turn-helix domains, which are bound to adjacent major grooves of the DNA. The DNA is kinked at the two recognition helix contact sites, and the DNA region between the kinks is straight. Among the major groove protein-bound DNAs, this kink-straight-kink bend contrasts with ordinary round bends (gradual bending between two protein contact sites). The larger kink (43 degrees ) is induced by a novel mechanism, phosphate bridging by an arginine-rich helix: the recognition helix with an arginine cluster is inserted perpendicularly into the major groove and bridges the groove through direct interactions with ...
We sell ELISA, IFA, RIA kits, antibody/antibodies for many antigens and hosts, IgA, IgD, IgE, IgG, IgM. Antibody application for WB, ELISA, IHC-P, IF, FCM.
Reactivity: Chicken, Cow, Dog and more. Compare 39 different CENPB ELISA Kits & buy the right one directly at antibodies-online.com!
A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance ...
The centromere protein B (CENP-B) is a centromeric DNA/binding protein. It recognizes a 17-bp sequence motif called the CENP-B box, which is found in the centromeric region of most chromosomes. It binds DNA through its amino terminus and dimerizes through its carboxy terminus. CENP-B protein has bee …
Two ZMPSTE24 mutations in the yeast to complement the (S. cerevisiae) mating defect STE24 and Ras-converting enzyme 1 (RCE1; another prenylprotein-specific endoprotease) genes [§§] (farnesylated protein-converting enzymes 1 and 2) is a significant component of the rice centromere antigens lamin A/C and B1 identified (in non-transgenic plants that go awry↩ (centromere) in cancer), the Ras…
Product Monkey Centromere protein R(ITGB3BP) ELISA kit From B-Gene - A sandwich ELISA for quantitative measurement of Monkey Centromere protein R(ITGB3BP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUTION*1 vial 12. WASH SOLUTION (100 x)*1 vial 13. BALANCE SOLUTION*1 vial 14. INSTRUCTION*1
In a subset of patients with limited cutaneous (lc) systemic sclerosis (SSc), anti-CENP-A antibodies (Ab) cross-react with a peptide (FOXE3p53-62) that presents striking homology with one of the two immunodominant epitopes of CENP-A (Ap17-30). We searched for clinical correlates of anti-FOXE3p53-62 Ab by measuring their levels along with those of Ab to Ap17-30 and to the second immunodominant epitope of CENP-A, namely Ap1-17. Serum samples were obtained from 121 patients with SSc, 46 patients with systemic lupus erythematosus (SLE) and 25 healthy blood donors (HBD). The reactivity of serum IgG to Ap1-17, Ap17-30 and FOXE3p53-62 was measured by ELISA. The corresponding anti-peptide Ab were affinity-purified from pooled SSc sera and used to establish standard curves for quantifying these Ab in patients and HBD. Receiver operating characteristics (ROC) analysis, comparing SSc patients who were positive for anti-CENP Ab (ACA+) to those who were negative, was used to find cut-off points for dichotomizing the
TY - GEN. T1 - Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis. AU - Perdoni, Federica. AU - Bottone, Maria Grazia. AU - Soldani, Cristiana. AU - Veneroni, Paola. AU - Alpini, Claudia. AU - Pellicciari, Carlo. AU - Scovassi, Anna Ivana. PY - 2009/8. Y1 - 2009/8. N2 - A large complex of proteins, called CENPs, are associated with centromeric DNA. Some of them exhibit a cell cycle-related expression (e.g., CENP-E and -F) and are required for the transition from interphase to mitosis, whereas constitutive proteins (e.g., CENP-A, -B, -C, -G, and -H) reside permanently at the centromere and are essential for the correct kinetochore assembly. Poly(ADP-ribose) polymerase-1 (PARP-1), which plays an active role in many basic processes, was described as a possible regulator of CENPs. By multicolor immunofluorescence we therefore analyzed the distribution of PARP-1 and its interaction with CENP-B, -E, and -F during mitosis and apoptosis.. AB - A large complex of proteins, ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Find MSDS or SDS, a COA, data sheets and more information.
Supplier: Elabscience Product Type: ELISA Kit Size: 96T Description: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CENPI . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Mouse monoclonal antibody raised against a full length recombinant CENPA. CENPA (AAH00881, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00001058-M01) - Products - Abnova
A process for preparing a polarizer is described whereby a pre-polarizing article comprising an oriented, vinylalcohol polymer film layer, and an acid donor layer comprising a thermal acid generator, is exposed to radiant energy at a temperature sufficient to effect a partial dehydration of the vinylalcohol polymer to a vinylalcohol/poly(acetylene) copolymer.
CENPA antibody [3-19] (centromere protein A) for ICC/IF, IHC, IHC-P, Neut, WB. Anti-CENPA mAb (GTX13939) is tested in Human, Chicken samples. 100% Ab-Assurance.
CENPT antibody (centromere protein T) for ELISA, ICC/IF, IHC-F, IHC-P, IP, WB. Anti-CENPT pAb (GTX51767) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Complete information for CENPP gene (Protein Coding), Centromere Protein P, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Gene target information for CENPV - centromere protein V (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Does anyone here have a set of these? http://www.ptreeusa.com/panel_glue_up.htm My wife gifted me two sets when they were on a 40% off sale. I havent used them yet but have mounted them to a 2x attached too a wall and together theyll give about 7 of clamping length. They claim to work from thin to up to 5 thick. When I first saw them I immediately thought of a benchtop to break them in. |g| Id planned on adding a Bessey K-type between each of these clamps, plus one or two on each end.
Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few centromere proteins are conserved in all of these four organisms and many are unique to the different organisms. The recent analysis of the centromere structure in the yeast S. pombe by electron microscopy and detailed immunofluorescence microscopy of ...
Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[5][6][7] In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and passenger (or transiently interacting) proteins.[8] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD. The term passenger proteins encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[9] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where ...
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008 ...
We found that the CENP‐A NAC/CAD network plays an important role in regulating MT-kinetochore attachment and promotes efficient chromosome alignment on the metaphase plate, but is dispensable for MT-attachment per se. Our data indicate that CENP‐A NAC/CAD is not required for loading of the KMN network and that it regulates chromosome congression independently of the MIND complex (see Figure 8B). It is important to note that these findings contradict previous models in which CENP‐A NAC/CAD is essential for the loading of the KMN subcomplexes MIND and NDC80 onto kinetochores (Liu et al, 2006; Okada et al, 2006; Kwon et al, 2007). At the functional level, such a linear model of kinetochore assembly is inconsistent with both our own and other CENP‐A NAC/CAD knockdown phenotypes; while NDC80 depletion results in MT attachment failure, loss of CENP‐H, CENP‐I, Chl4R, CENP‐U or CENP‐T allows the formation of a partial metaphase plate, which requires stable attachment and ...
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. To examine the molecular basis for the specification of centromeric chromatin, we have cloned a human cDNA that encodes the 17-kD histone-like centromere antigen, CENP-A. Two domains are evident in the 140 aa CENP-A polypeptide: a unique NH2-terminal domain and a 93-amino acid COOH-terminal domain that shares 62% identity with nucleosomal core protein, histone H3. An epitope tagged derivative of CENP-A was faithfully targeted to centromeres when expressed in a variety of animal cells and this targeting activity was shown to reside in the histone-like COOH-terminal domain of CENP-A. These data clearly indicate that the assembly of centromeres is driven, at least in part, by the incorporation of a novel core histone into centromeric chromatin. ...
Accurate chromosome segregation requires the assembly of kinetochores, multiprotein complexes that assemble on the centromere of each sister chromatid. A key step in this process involves binding of the constitutive centromere‐associated network (CCAN) to CENP‐A, the histone H3 variant that constitutes centromeric nucleosomes. This network is proposed to operate as a persistent structural scaffold for assembly of the outer kinetochore during mitosis. Here, we show by fluorescence resonance energy transfer (FRET) that the N‐terminus of CENP‐N lies in close proximity to the N‐terminus of CENP‐A in vivo, consistent with in vitro data showing direct binding of CENP‐N to CENP‐A. Furthermore, we demonstrate in living cells that CENP‐N is bound to kinetochores during S phase and G2, but is largely absent from kinetochores during mitosis and G1. By measuring the dynamics of kinetochore binding, we reveal that CENP‐N undergoes rapid exchange in G1 until the middle of S phase when it ...
UA62784 is a specific inhibitor of centromere protein E (CENPE) kinesin-like protein; mitotic inhibitor. CENP-E is a kinesin-like motor protein that localizes to the kinetochore during mitosis and is essential for bipolar spindle formation. UA62784 is a novel specific inhibitor of CENP-E, which likely binds within the motor domain of CENP-E. UA62784 causes reversible cell cycle arrest in mitosis before metaphase, which leads to apoptosis. The compound is not interacting with microtubules directly. (Last Updated: 6/22/2015)
It has recently been proposed that mitotic chromosomes transport certain cytoskeletal proteins to the metaphase plate so that these proteins are able to subsequently participate in the assembly of the anaphase spindle and the cleavage furrow. To understand how such proteins accomplish their dual chromosomal: cytoskeletal role, we have begun a molecular and functional analysis of the inner centromere proteins (INCENPs), founder members of the class of chromosome passenger proteins. cDNA clones encoding the open reading frames of the two chicken INCENPs were recovered. The predicted proteins, class I INCENP (96,357 D) and class II INCENP (100,931 D) are novel, and differ from each other by the inclusion of a 38-codon insert within the class II coding region. Transient expression of the chicken INCENPs in mammalian cells confirms that the signals and structures required for the transfer of these proteins from chromosomes to cytoskeleton are evolutionarily conserved. Furthermore, these studies ...
The centromere is a specialized chromosomal region that directs the formation of the kinetochore, a huge protein assembly that acts as the attachment site for spindle microtubules and carries out chromosome movement during cell division. Centromere loss or the presence of extra centromeres adversely affect chromosome segregation and may result in aneuploidy, a condition found in many human tumors and a major cause of miscarriages and birth defects. Consequently, understanding the basis of centromere determination and propagation is of great relevance to both fundamental and clinical research. In recent years, it has become clear that centromeres are defined by the presence of a histone H3 variant known as Centromere Protein A, CENP-A, or CenH3. Much effort has been devoted to understanding the mechanisms that drive the assembly of CENP-A containing nucleosomes exclusively onto centromeric DNA, as well as the peculiar structure of these nucleosomes. We have recently developed an immunofluorescence-based
1989) and may interact with tubulin (Balczon and Brinkley, 1987). Simerly et al. (1990) suggested that the CENP-B protein could be involved in either microtubule assembly at the kinetochore or in sliding of the kinetochore along the microtubules. ,1990). Injection of these purified IgGs into tissue culture cells disrupts microtubule attachment to kinetochores, chromosome congression, and chromosome segregation, depending on the stage of the cell cycle at the time of injection. The authors suggest that most of their results can be explained by defects in the assembly or maturation of the kinetochores such that either microtubules do not attach to the kinetochores or the attachment is defective. Other investigators have also reported the presence of Ca2-binding, contractile proteins in the centrosome, but have suggested other functions for the proteins. Baron et al. (1991) have suggested that the Ca2-binding, centrosomal proteins in marsupial and mammalian cell lines, identified by antibodies ...
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM;117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006;(PubMed 16622420 ...
In most eukaryotes, centromeres are epigenetically defined by nucleosomes that contain the histone H3 variant centromere protein A (CENP-A). Specific targeting of the CENP-A-loading chaperone to the centromere is vital for stable centromere propagation; however, the existence of ectopic centromeres …
The histone protein CenH3 is both necessary and sufficient to trigger the formation of centromeres and pass them on from 1 generation to the next. Centromeres are specialised regions of the genome, which can be identified under the microscope as the primary constriction in X-shaped chromosomes. The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the centromeres. In most organisms the position of the centromere is not determined by the DNA sequence. Scientists from the Max Planck Institute of Immunobiology and Epigenetics in Freiburg have succeeded in demonstrating that the position, function and inheritance of the centromere are determined by the histone CenH3, a DNA packaging protein. This discovery may help to further the development of artificial human chromosomes, which could be used for gene therapies in medicine.. Centromeres provide a platform for the development of a protein complex known as the kinetochore. During cell ...
Large-scale genome rearrangements brought about by chromosome breaks underlie numerous inherited diseases, initiate or promote many cancers and are also associated with karyotype diversification during species evolution. Recent research has shown that these breakpoints are nonrandomly distributed throughout the mammalian genome and many, termed evolutionary breakpoints (EB), are specific genomic locations that are reused during karyotypic evolution. When the phylogenetic trajectory of orthologous chromosome segments is considered, many of these EB are coincident with ancient centromere activity as well as new centromere formation. While EB have been characterized as repeat-rich regions, it has not been determined whether specific sequences have been retained during evolution that would indicate previous centromere activity or a propensity for new centromere formation. Likewise, the conservation of specific sequence motifs or classes at EBs among divergent mammalian taxa has not been determined. To
DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation ...
Phenotype data for mouse gene Cenpo. Discover Cenpos significant phenotypes, expression, images, histopathology and more. Data for gene Cenpo is all freely available for download.
Academic Journals Database is a universal index of periodical literature covering basic research from all fields of knowledge, and is particularly strong in medical research, humanities and social sciences. Full-text from most of the articles is available. Academic Journals Database contains complete bibliographic citations, precise indexing, and informative abstracts for papers from a wide range of periodicals.
Replication of alpha-satellite DNA arrays in endogenous human centromeric regions and in human artificial chromosome Indri Erliandri, Haiqing Fu, Megumi Nakano, Jung-Hyun Kim, Karen H. Miga,Mikhail Liskovykh, William C. Earnshaw, Hiroshi Masumoto, Natalay Kouprina, Mirit I. Aladjem, and Vladimir Larionov Nucl. Acids Res. 2014 42: 11502-11516 ...
THE centromeres in most plants and animals contain hundreds of kilobases of simple repeats and interspersed retroelements. Such repetitive domains evolve at remarkable rates-continually expanding, contracting, and generating new arrays (Schueler et al. 2001; Henikoff 2002; Nagaki et al. 2004; Lee et al. 2005; Ma and Bennetzen 2006). This process tends to homogenize centromeres and drive out single- and low-copy sequences that are necessary for sequencing and molecular-marker-based mapping (Henikoff 2002; Dawe 2005). Two relatively simple rice centromeres have been bridged and characterized (Nagaki et al. 2004; Wu et al. 2004; Zhang et al. 2004), but as a rule centromeres fall into large and poorly resolved genetic gaps. In maize, the centromere positions are estimates based on rough interval or trisomic mapping and visual comparisons to the cytogenetic map (Weber and Helentjaris 1989; Schneerman et al. 1998; Lin et al. 2001).. Another complexity of centromere mapping is that sequence alone ...
rs438034 is a SNP in the centromere protein F CENPF gene. Based on a long-term (up to 15 years) study of 749 Swedish women with breast cancer, carriers of rs438034(T) alleles (as oriented wrt dbSNP) had poorer specific survival rates as compared to rs438034(C;C) individuals. The hazard ratio was 2.65 (CI: 1.19-5.90). However, although rs438034(T) carriers had worse survival odds, they were less likely to have either regional lymph node metastases (odds ratio 0.71, CI: 0.51-1.01) or tumors of stage II-IV (OR 0.73, CI 0.54-0.99). [PMID 19008095] ...
Centromere B Ab ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of IgG class autoantibodies against Centromere B in human serum or plasma. (KA1275) - Products - Abnova
Centromere   The centromere is a region in the middle of the chromosome involved in cell division and the control of gene expression. Additional
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
The Bioconductor-mirror/GWASTools package contains the following man pages: alleleFrequency allequal anomDetectBAF anomDetectLOH anomIdentifyLowQuality anomSegStats apartSnpSelection assocCoxPH assocRegression BAFfromClusterMeans BAFfromGenotypes batchTest centromeres chromIntensityPlot convertNcdfGds createDataFile defunct duplicateDiscordance duplicateDiscordanceAcrossDatasets duplicateDiscordanceProbability exactHWE findBAFvariance GdsGenotypeReader-class GdsIntensityReader-class GdsReader-class gdsSubset genoClusterPlot GenotypeData-class genotypeToCharacter getobj getVariable GWASTools-package hetByScanChrom hetBySnpSex HLA ibdPlot imputedDosageFile IntensityData-class intensityOutliersPlot manhattanPlot MatrixGenotypeReader-class meanIntensityByScanChrom mendelErr mendelList missingGenotypeByScanChrom missingGenotypeBySnpSex NcdfGenotypeReader-class NcdfIntensityReader-class NcdfReader-class pasteSorted pcaSnpFilters pedigreeCheck pedigreeDeleteDuplicates pedigreeMaxUnrelated
Author Summary The centromere is a chromosome domain essential for the correct partitioning of chromosomes during mitotic and meiotic cell divisions. The characterization of the centromeric proteins and their sequential assembly have been extensively studied in mammalian mitosis, since defective chromosome segregation is associated with birth defects and cancer. However, few studies have analyzed the centromere assembly during meiosis, a special cell division leading to the production of haploid gametes. Here, we analyze the sequence of loading of several centromeric and kinetochoric proteins during male mouse meiosis. We show that during both meiotic divisions, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The outer kinetochore proteins BubR1 and CENP-E are the last ones to be assembled. We also demonstrate, using a knockout mouse for Sgol2, that the inner centromeric protein Shugoshin 2 is
Centromeres are unique chromatin domains that direct the site of kinetochore formation during mitosis and mediate the movement of chromosomes during cell division. Centromeres contain a unique nucleosome in which histone H3 is replaced by centromere protein A (CENP-A). Because of their unique position in chromatin, the CENP-A nucleosome was hypothesized to determine the site of centromere and kinetochore assembly. In order to test the long held assumption that CENP-A dictates the location of the centromere; we developed a novel de novo centromere formation assay, which provides a new and powerful constructive approach to studying centromeres. This system is based on a LacO array that is stably integrated into the long arm of chromosome 1, far away from the existing centromere. Targeting the CENP-A chaperone HJURP or the Mis18 complex to the LacO array, by fusing them to the LacI repressor, drove the stable recruitment of CENP-A nucleosomes to the LacO array at the non-centromeric locus. ...
DNA methylation is an epigenetically imposed mark of transcriptional repression that is essential for maintenance of chromatin structure and genomic stability. Genome-wide methylation patterns are mediated by the combined action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B. Compelling links exist between DNMT3B and chromosome stability as emphasized by the mitotic defects that are a hallmark of ICF syndrome, a disease arising from germline mutations in DNMT3B. Centromeric and pericentromeric regions are essential for chromosome condensation and the fidelity of segregation. Centromere regions contain distinct epigenetic marks, including dense DNA hypermethylation, yet the mechanisms by which DNA methylation is targeted to these regions remains largely unknown. In the present study, we used a yeast two-hybrid screen and identified a novel interaction between DNMT3B and constitutive centromere protein CENP-C. CENP-C is itself essential for mitosis. We confirm this interaction in ...
Ovarian cancer has a poor prognosis. Most patients are diagnosed with ovarian cancer when the disease has reached an advanced stage and cure rates are generally under 30%. Hence, early diagnosis of ovarian cancer is the best means to control the disease in the long term and abate mortality. So far, cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) are the gold-standard tumor markers for ovarian cancer; however, these two markers can be elevated in a number of conditions unrelated to ovarian cancer, resulting in decreased specifically and positive predictive value. Therefore, it is urgent to identify novel biomarkers with high reliability and sensitivity for ovarian cancer. In this study for the first time, we identified a member of the centromere protein (CENP) family, CENPK, which was specifically upregulated in ovarian cancer tissues and cell lines and the overexpression of which was associated with poor prognoses in patients with ovarian cancer. In addition, the presence of CENPK
The HTR12 protein is a centromere-specific histone H3 variant in A. thaliana, and was shown to colocalize with the 180 bp repetitive sequences of all centromeres (Talbert et al., 2002). The Zea mays centromeric histone H3, CENH3 was also detected at the kinetochore regions of the centromere and colocalized with centromere-specific tandem repeat CentC and with centromeric retroelement CRM (Zhong et al., 2002). These results indicate wide conservation of CENP-A-like proteins and their close relationship to the centromeric satellites. In our study, the spatial relationship between HTR12 protein and 180 bp repetitive sequences was investigated by sequential combination of immunolabeling and FISH. In the cell cultures studied here, drastic changes in the copy numbers of 180 bp repetitive sequences had occurred, however, all chromosomes carried the 180 bp repetitive sequences despite their variation in size (Fig. 1E,F). For chromosomes with low numbers of 180 bp repetitive sequences, ...
Saffery, Richard, Sumer, Huseyin, Hassan, Sara, Wong, Lee H, Craig, Jeffrey M, Todokoro, Kazuo, Anderson, Melissa, Stafford, Angela and Choo, KH Andy 2003, Transcription within a functional human centromere, Molecular cell, vol. 12, no. 2, pp. 509-516, doi: 10.1016/S1097-2765(03)00279-X. ...
The centromere facilitates the assembly of the kinetochore ensuring the accurate chromosome segregation. Active centromeres are specified epigenetically by nucleosomes containing the histone H3 variant, CENP-A, which is in place of histone H3. CENP-A is deposited by an assembly factor called HJURP in humans and Scm3 in yeast. However, homologs of HJURP/Scm3 are only present in a subset of eukaryotes. How CENP-A is deposited exclusively at centromeres in organisms that lack CENP-A chaperones remains unknown. This thesis addresses the above-mentioned gaps in the understanding of CENP-A assembly and identifies CAL1 as an essential recruiter for Drosophila CENP-A, which fulfills the function of HJURP/Scm3 in the dipteran lineage. Mis-targeting CAL1 is sufficient to trigger the formation of a functional centromere at the ectopic site, where the recruitment of centromeric proteins and microtubule attachments are detected. This additional centromere can be propagated epigenetically to the next generation and
GF ID Scm3 #=GF AC PF10384.8 #=GF DE Centromere protein Scm3 #=GF AU Mistry J, Wood V #=GF SE Pfam-B_19394 (release 21.0) #=GF GA 24.30 24.30; #=GF TC 24.60 24.40; #=GF NC 24.20 24.00; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 17548816 #=GF RT Scm3, an essential Saccharomyces cerevisiae centromere protein #=GF RT required for G2/M progression and Cse4 localization. #=GF RA Stoler S, Rogers K, Weitze S, Morey L, Fitzgerald-Hayes M, Baker #=GF RA RE; #=GF RL Proc Natl Acad Sci U S A. 2007;104:10571-10576. #=GF RN [2] #=GF RM 17704645 #=GF RT Domain Architectures of the Scm3p Protein Provide Insights into #=GF RT Centromere Function and Evolution. #=GF RA Aravind L, Iyer LM, Wu C; #=GF RL Cell Cycle. 2007; [Epub ahead of print] #=GF RN [3] #=GF RM 19563746 #=GF RT Common ancestry of the CENP-A chaperones Scm3 and HJURP. #=GF RA Sanchez-Pulido L, Pidoux AL, Ponting CP, Allshire RC; #=GF RL Cell. 2009;137:1173-1174. ...
Centromeres are the chromosomal regions that link DNA to the spindle during cell division, thus ensuring faithful segregation of genetic material. Proper centromere function is critical for eukaryotic life. Despite the fact that centromeres are essential for life, centromeric architecture is remarkably diverse. Moreover, centromeric DNA sequences and centromeric proteins evolve rapidly in diverse organisms. Despite this, the hallmark of many functional centromeres is the presence of a specialized centromeric H3 variant called CenH3. Despite being essential for chromosome segregation in most eukaryotes, CenH3 also evolves rapidly in plants and animals. I study the rapidly evolution of CenH3 in Drosophila. Specifically, I study instances in which CenH3 has duplicated and may have undergone subfunctionalization. I am intrigued by the possibility that these duplications may have arisen in response to genetic conflict such as centromere drive or due to intralocus antagonism. I use a variety of ...
TY - JOUR. T1 - Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction. AU - Luce, Amy C.. AU - Sharma, Anupma. AU - Mollere, Oliver S.B.. AU - Wolfgruber, Thomas K.. AU - Nagaki, Kiyotaka. AU - Jiang, Jiming. AU - Presting, Gernot G.. AU - Dawe, R. Kelly. PY - 2006/11/6. Y1 - 2006/11/6. N2 - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.. AB - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Buy UA62784 (CAS 313367-92-9), a centromere protein E (CENP-E) inhibitor, from Santa Cruz. Purity: ≥98%, Molecular Formula: C23H15NO3, MW: 353.37
Experimental stimulation of TLR9 often utilizes phosphorothioate (PS)-ODN, in which one of the backbone oxygens is replaced by sulfur, rendering these ODN more nuclease-resistant than natural ODN with a phosphodiester (PD) backbone. These ODN make effective artificial ligands and can be divided into Class A (also known as D-type), ClassB (also known as K-type), and Class C ODNs; each with distinct immunological effects (5). CpG-A and CpG-C ODNs are strong inducers of type I interferons (6), particularly in plasmacytoid dendritic cells (pDCs),whereas CpG-B ODNs are weak inducers of IFN-αβ (5;7;8), but they induce DCs to mature and produce the tumor necrosis factor (TNF)-α cytokine (5;7) ...
The centromere is the structure at the center of every X-shaped chromosome, where cells attached the long, thin spindles that pull the two copies of DNA apart during cell division. A new technique makes it much easier to study centromeres, and look for links to conditions such as Down syndrome.
Informaţii generale Bolile autoimune ale ficatului includ: • Hepatita autoimună (AIH) • Ciroza biliară primitivă (PBC) • Colangita sclerozantă primitivă (PSC).
Plasmid mRuby2-CENPB-N-22 from Dr. Michael Davidsons lab contains the insert CENPB and is published in Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. This plasmid is available through Addgene.
Words starting with C (page 38): centripetally, centrism, centrist, centrobaric, centroclinal, centrode, centroid, centroidal, centroids, centrolecithal, centromere, centromeric, centrosome, centrosomic, centrosphere, centrosymmetric, centrum, centry, cents, centu...
The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD. The term 'passenger proteins' ... two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and ' ... Inner centromere protein is a protein that in humans is encoded by the INCENP gene. In mammalian cells, ... "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa". Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire ...
Centromere protein C 1 is a protein that in humans is encoded by the CENPC1 gene. Centromere protein C 1 is a centromere ... 2002). "Mutational analysis of the central centromere targeting domain of human centromere protein C, (CENP-C)". Exp. Cell Res ... 2004). "In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: ... "Interphase-specific association of intrinsic centromere protein CENP-C with HDaxx, a death domain-binding protein implicated in ...
Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene. This gene encodes a ... The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... "Conservation of the centromere/kinetochore protein ZW10". J Cell Biol. 138 (6): 1289-301. doi:10.1083/jcb.138.6.1289. PMC ...
Centromere protein O is a protein that in humans is encoded by the CENPO gene. CENPO is involved in cell proliferation and cell ... "Entrez Gene: CENPO centromere protein O". Hewitt, Chelsee A.; Ling, King-Hwa; Merson, Tobias D.; Simpson, Ken M.; Ritchie, ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...
Centromere protein F is a protein that in humans is encoded by the CENPF gene. It is involved in chromosome segregation during ... the protein forms part of the kinetochore, a disc-shaped protein complex that allows the centromere of two sister chromatids to ... "Entrez Gene: CENPF centromere protein F, 350/400ka (mitosin)". Waters, Aoife M.; Asfahani, Rowan; Carroll, Paula; Bicknell, ... "The cloning and analysis of LEK1 identifies variations in the LEK/centromere protein F/mitosin gene family". J. Biol. Chem. 274 ...
The second is a DNA-binding adaptor protein known as ParR. The last component is a centromere-like region called ParC. The ... The three components, a parC DNA site, and two proteins parR and parM all combine to create the ParMRC system, a type II ... In the cell the ParM protein filaments search for plasmids. Next, they find the ParR and ParC that are headed to DNA molecules ... It has three components: ParM, an actin-like protein that forms a long filament to push two plasmids apart, ParR, which binds ...
This alternative form is composed of core RFC proteins RFC2, RFC3, RFC4, and RFC5, but replaces the RFC1 protein with cohesion ... High levels of cohesin binding are observed at the centromere. Cohesin is also loaded at cohesin attachment regions (CARs) ... SMC1ß, REC8 and STAG3 are meiosis specific cohesin proteins. The STAG3 protein is essential for female meiosis and fertility. A ... The cohesin ring is composed of two SMC (structural maintenance of chromosomes) proteins and two additional Scc proteins. ...
Centromere protein H is a protein that in humans is encoded by the CENPH gene. Centromere and kinetochore proteins play a ... "Entrez Gene: CENPH centromere protein H". Fukagawa, T.; Mikami, Y.; Nishihashi, A.; Regnier, V.; Haraguchi, T.; Hiraoka, Y.; ... 2005). "Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy". ... 2007). "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival ...
Anti-centromere antibodies are common in limited systemic sclerosis (CREST syndrome). Nail fold vasculature (capillaroscopy) ... Tests for rheumatoid factor, erythrocyte sedimentation rate, C-reactive protein, and autoantibody screening may reveal specific ...
Diseases with ATA are autoimmune disease because they react with self-proteins. They are also referred to as anti-DNA ... However, CREST syndrome is more closely associated with anti-centromere antibodies. Scl-70 antibodies are associated with more ... In addition to HLA-DR, the protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (1p13.2 - PTPN22), "CT/TT" genotype ... 2006). "Association of the protein tyrosine phosphatase, non-receptor type 8 R620W polymorphism with anti-topoisomerase I- and ...
Centromere protein M also known as proliferation associated nuclear element 1 (PANE1) is a protein that in humans is encoded by ... Centromere protein M is involved in centromere assembly and immune response. GRCh38: Ensembl release 89: ENSG00000100162 - ... "Entrez Gene: CENPM centromere protein M". Brickner AG, Evans AM, Mito JK, Xuereb SM, Feng X, Nishida T, Fairfull L, Ferrell RE ... Overview of all the structural information available in the PDB for UniProt: Q9NSP4 (Human Centromere protein M (CENPM)) at the ...
"Distinct protein interaction domains and protein spreading in a complex centromere". Genes & Development. 14 (7): 783-91. PMC ... Related Protein, Sim3, Binds CENP-A and is Required for Its Deposition at Fission Yeast Centromeres". Molecular Cell. 28 (6): ... Ekwall, K; Javerzat, J. P; Lorentz, A; Schmidt, H; Cranston, G; Allshire, R (1995). "The chromodomain protein Swi6: A key ... Allshire, R. C; Javerzat, J. P; Redhead, N. J; Cranston, G (1994). "Position effect variegation at fission yeast centromeres". ...
An important reference point along a chromosome is the centromere; the distance from a gene to the centromere is referred to as ... The ABO blood group proteins are important in determining blood type in humans, and this is determined by different alleles of ... A gene is a portion of a chromosome, which is a very long and compacted string of DNA and proteins. ... The biochemistry of the intermediate proteins determines how they interact in the cell. Therefore, biochemistry predicts how ...
Centromere protein A, also known as CENPA, is a protein which in humans is encoded by the CENPA gene. CENPA is a histone H3 ... "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated". ... CENPA is a protein which epigenetically defines the position of the centromere on each chromosome, determining the position of ... Uren AG, Wong L, Pakusch M, Fowler KJ, Burrows FJ, Vaux DL, Choo KH (November 2000). "Survivin and the inner centromere protein ...
... s carry out essential functions with Shugoshin protein. Shugoshin is a centromere protein for chromosome segregation ... In response to this, the chromosome could induce centromere inactivation to impede the formation of two centromeres, but this ... Telomeres are specialized protein-DNA constructs present at the ends of eukaryotic chromosomes, which prevent them from ... There are two types of Shugoshin protein: SGOL1 and SGOL2. Sgo1 is only expressed in meiosis 1 for centromeric cohesion of the ...
Microtubules attach to the midpoint of chromosomes (the centromere) via protein complexes (kinetochores). The attached ... The centromeres are split, and the sister chromatids are pulled toward the poles by kinetochore microtubules. They take on a V- ... Securin is a protein which inhibits a protease known as separase. The destruction of securin unleashes separase which then ... and by motor proteins such as dyneins or kinesins. Anaphase accounts for approximately 1% of the cell cycle's duration. It ...
The regulation of transcription factors, effector proteins, molecular chaperones, and cytoskeletal proteins by acetylation and ... Acetylation of histone H4K5 and H4K12ac is enriched at centromeres. H4K5ac indicates acetylation of lysine 5 on histone H4 ... Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. ... It results in good optimization and is used in vivo to reveal DNA-protein binding occurring in cells. ChIP-Seq can be used to ...
... is a protein related to the beta subunit of heterotrimeric G proteins. Near its C-terminus it contains seven WD40 repeats ... The checkpoint is held active by any unattached centromere; only when all centromeres are attached will anaphase commence. The ... Securin is a protein that inhibits separase, which in turn inhibits cohesin, a protein that holds sister chromatids together. ... CDC20 appears to act as a regulatory protein interacting with many other proteins at multiple points in the cell cycle. It is ...
Jiang W, Koltin Y (1996). "Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae ... For example, sumoylation may affect a protein's localization in the cell, its ability to interact with other proteins or DNA. ... SENP proteases can remove SUMO from sumoylated proteins, freeing it to be used in further sumoylation reactions. The protein ... Four alternatively spliced transcript variants encoding the same protein have been found for this gene. The UBC9 protein ...
The protein localizes primarily to the nucleus and its expression is developmentally regulated. Eight alternatively spliced ... Mutation in this gene are associated with immunodeficiency, centromere instability and facial anomalies syndrome. CpG ...
3-9 encode centromere-associated proteins which complex with the heterochromatin component M31". The EMBO Journal. 18 (7): 1923 ... Chromobox protein homolog 1 is a protein that in humans is encoded by the CBX1 gene. The protein is localized at ... 3-9 encode centromere-associated proteins which complex with the heterochromatin component M31". The EMBO Journal. 18 (7): 1923 ... "The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the regulation of ...
Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. The protein encoded by this gene is a ... Kim IG, Jun DY, Sohn U, Kim YH (Feb 1998). "Cloning and expression of human mitotic centromere-associated kinesin gene". ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be ...
Evidence suggests that binding of cohesin to the budding yeast centromere region depends on chromosome-associated proteins of ... SMC1β, REC8 and STAG3 proteins are meiosis specific cohesins. The STAG3 protein appears to be essential for female meiosis. A ... the presence of repetitive heterochromatic DNA in centromeres and the presence of chromosome-associated proteins. For example, ... Several proteins aid in the loading process. For example, Scc2 and Scc4 are both required for cohesin to load in budding yeast ...
Centromere protein R is a protein that in humans is encoded by the ITGB3BP gene. ITGB3BP has been shown to interact with: CD61 ... Talukder AH, Gururaj A, Mishra SK, Vadlamudi RK, Kumar R (2004). "Metastasis-associated protein 1 interacts with NRIF3, an ... "Entrez Gene: ITGB3BP integrin beta 3 binding protein (beta3-endonexin)". Fujimoto TT, Katsutani S, Shimomura T, Fujimura K ( ... PKC theta-dependent protein". J. Cell Biol. 147 (5): 1073-84. doi:10.1083/jcb.147.5.1073. PMC 2169340. PMID 10579726. Ohtoshi A ...
Centromere protein K is a protein that in humans is encoded by the CENPK gene. CENPK has been shown to interact with SOX6. ... "Entrez Gene: CENPK centromere protein K". Yamashita A, Ito M, Takamatsu N, Shiba T (Sep 2000). "Characterization of Solt, a ... Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (Feb 2004). "Proteomics analysis of the centromere complex from HeLa ... Yamashita A, Ito M, Takamatsu N, Shiba T (Sep 2000). "Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in ...
Centromere protein J is a protein that in humans is encoded by the CENPJ gene. It is also known as centrosomal P4.1-associated ... "Entrez Gene: CENPJ centromere protein J". Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, ... Overview of all the structural information available in the PDB for UniProt: Q9HC77 (Centromere protein J) at the PDBe-KB.. ... Hung LY, Tang CJ, Tang TK (Oct 2000). "Protein 4.1 R-135 interacts with a novel centrosomal protein (CPAP) which is associated ...
Centromere protein I is a protein that in humans is encoded by the CENPI gene. The product of this gene is involved in the ... "Entrez Gene: CENPI centromere protein I". Thangavelu PU, Lin CY, Vaidyanathan S, Nguyen TH, Dray E, Duijf PH (September 2017 ... Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (February 2004). "Proteomics analysis of the centromere complex from HeLa ... Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells". Genes to Cells. 11 (6): 673-84. doi ...
Centromere protein L is a protein that in humans is encoded by the CENPL gene. GRCh38: Ensembl release 89: ENSG00000120334 - ... "Entrez Gene: CENPL centromere protein L". Human CENPL genome location and CENPL gene details page in the UCSC Genome Browser. ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human ...
Centromere protein T is a protein that in humans is encoded by the CENPT gene. Mutations in CENPT cause an autosomal recessive ... "Entrez Gene: CENPT centromere protein T". "OMIM Entry - # 618702 - SHORT STATURE AND MICROCEPHALY WITH GENITAL ANOMALIES; SSMGA ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human ...
Centromere protein N is a protein that in humans is encoded by the CENPN gene. GRCh38: Ensembl release 89: ENSG00000166451 - ... "Entrez Gene: CENPN centromere protein N". Human CENPN genome location and CENPN gene details page in the UCSC Genome Browser. ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres". Nat. ...
UNKL: encoding protein RING finger protein unkempt-like. *VAT1L: encoding protein Vesicle amine transport protein 1 homolog (T ... acen Centromere. var: Variable region; stalk: Stalk. ... ITFG3: encoding protein Protein ITFG3. *KDM8: encoding protein ... LINC00273 encoding protein Long intergenic non-protein coding RNA 273. *LOC124220: encoding protein Zymogen granule protein 16 ... SHCBP1: encoding protein SHC SH2 domain-binding protein 1. *SLZ1: encoding protein SLX1 structure-specific endonuclease subunit ...
... who believed that transcription was activated by protein-DNA and protein-protein interactions on largely naked DNA templates, ... For example, histone H3-like CENPA is associated with only the centromere region of the chromosome. Histone H2A variant H2A.Z ... Nuclear protein Ataxia-Telangiectasia (NPAT), also known as nuclear protein coactivator of histone transcription, is a ... The first step of chromatin structure duplication is the synthesis of histone proteins: H1, H2A, H2B, H3, H4. These proteins ...
In APS there are also antibodies binding to Protein S, which is a co-factor of protein C. Thus, anti-protein S antibodies ... Protein C deficiency/Activated protein C resistance/Protein S deficiency/Factor V Leiden ... Levels of protein C, free and total protein S, factor VIII, antithrombin, plasminogen, tissue plasminogen activator (TPA) and ... Anti-ApoH and a subset of anti-cardiolipin antibodies bind to ApoH, which in turn inhibits Protein C, a glycoprotein with ...
... surgical techniques are used to remove an invasive malignancy that extends to the clitoris. Standard surgical procedures are followed in these cases. This includes evaluation and biopsy. Other factors that will affect the technique selected are age, other existing medical conditions, and obesity. Other considerations are the probability of extended hospital care and the development of infection at the surgical site.[3] The surgery proceeds with the use of general anesthesia, and prior to the vulvectomy/clitoridectomy an inguinal lymphyadenectomy is first done. The extent of the surgical site extends one to two centimeters beyond the boundaries of malignancy. Superficial lymph nodes may also need to be removed. If the malignancy is present in muscular tissue in the region, it is also removed. In some cases, the surgeon is able to preserve the clitoris though the malignancy may be extensive. The cancerous tissue is removed and the incision is closed.[3] Post operative care may ...
The resulting BLM protein is defective. the defect in RecQ an helicase facilitates the defective unwinding of DNA during ... FRT sites have been inserted transgenically near the centromere of each chromosome arm of Drosophila melanogaster. The FLP gene ... The recombination needs to occur between the centromere the adjacent gene. This gives an appearance of yellow patches on the ... commonly the green fluorescent protein or GFP) and an allele of a gene to be studied (both on chromosomes bearing FRT sites). ...
... anti-centromere antibodies and anti-sp100 antibodies. Each of these antibody subtypes binds to different proteins or protein ... CENP-E is a 312kDa protein from the kinesin motor protein family. CENP-F is a 367kDa protein from the nuclear matrix that ... 54kDa and 60kDa proteins and RNA. The 60kDa DNA/RNA binding protein and 52kDa T-cell regulatory protein are the best ... The retroviral gag protein shows similarity to the La protein and is proposed as a possible example for molecular mimicry in ...
So CCDS's gene number prediction represents a lower bound on the total number of human protein-coding genes.[49] ... acen Centromere. var: Variable region; stalk: Stalk. ... DFNY1 encoding protein Deafness, Y-linked 1. *PRKY (protein ... at least 72 of which code for proteins.[5] Traits that are inherited via the Y chromosome are called Y-linked traits, or ... with between 45 and 73 of these protein-coding. All single-copy Y-linked genes are hemizygous (present on only one chromosome) ...
Chromosome duplication results in two identical sister chromatids bound together by cohesin proteins at the centromere. ... Motor proteins then push the centrosomes along these microtubules to opposite sides of the cell. Although centrosomes help ... Volume 15 of Protein Reviews. Berlin: Springer Science & Business Media. p. 15. ISBN 9781461405146.. ... The two chromatids are joined at the centromere. Gene transcription ceases during prophase and does not resume until late ...
In fission yeast this complex contains argonaute, a chromodomain protein Chp1, and a protein called Tas3 of unknown function.[ ... pombe disrupts histone methylation and centromere formation,[55] causing slow or stalled anaphase during cell division.[56] In ... This ancestral RNAi system probably contained at least one dicer-like protein, one argonaute, one PIWI protein, and an RNA- ... Left: A full-length argonaute protein from the archaea species Pyrococcus furiosus. Right: The PIWI domain of an argonaute ...
RecQ protein-like helicases (RECQLs), nucleotide excision repair (NER) proteins, and nuclear envelope proteins LMNA (lamins) ... are held together through a structure called the centromere. During this time, the homologous (corresponding) copies are in ... The Bloom syndrome protein interacts with other proteins, such as topoisomerase IIIα and RMI2,[28][29][30] and suppresses ... NER protein-associated PS[edit]. Further information: Nucleotide excision repair. Nucleotide excision repair is a DNA repair ...
Antisense nucleic acids and proteins: fundamentals and applications. pp. 4, 136. ISBN 0824785169.. ... "RNA interference is required for normal centromere function in fission yeast". Chromosome Res 11 (2): 137-46. PMID 12733640. ... Nakayashiki H, Kadotani N, Mayama S (2006). "Evolution and diversification of RNA silencing proteins in fungi". J Mol Evol 63 ( ... Tomari Y, Matranga C, Haley B, Martinez N, Zamore P (2004). "A protein sensor for siRNA asymmetry". Science 306 (5700): 1377-80 ...
Stevens FJ, Solomon A, Schiffer M (1991). "Bence Jones proteins: a powerful tool for the fundamental study of protein chemistry ... and their realization that this protein is the same as the Bence-Jones protein described in 1845 by Henry Bence Jones.[101] ... An antibody (Ab), also known as an immunoglobulin (Ig),[1] is a large, Y-shaped protein produced mainly by plasma cells that is ... and produce different intracellular and secretable proteins.[71] They are also used in immunoprecipitation to separate proteins ...
Since assembly of kinetochore proteins at centromeres is affected by the methylation of cytosine and histone proteins, a ... irregularities in kinetochore proteins or their assembly, dysfunctional spindle apparatus, or flawed anaphase checkpoint genes. ... centromere instability, and facial anomalies syndrome) or after treatment by DNA methyl transferase inhibitors. ...
C3orf14-Chromosome 3 open reading frame 14: predicted DNA binding protein.. *C3orf23: encoding protein Uncharacterized protein ... acen Centromere. var: Variable region; stalk: Stalk. ... PHF7 encoding protein PHD finger protein 7. *PTHR1: parathyroid ... FRA3A encoding protein Fragile site, aphidicolin type, common, fra(3)(p24.2). *FRMD4B encoding protein FERM domain containing ... ZBED2: encoding protein Zinc finger BED-type containing 2. *ZNF9: zinc finger protein 9 (a cellular retroviral nucleic acid ...
Dosadašnje studije na hromosomu 11 pokazuju 11,6 gena po baznom paru za 1.524 protein-kodirajućih gena i 765 pseudogena .[1] ... Prema položaju centromere ,pripada kategoriji submeracentričnih hromosoma. Sadrži oko 134,5 miliona baznih parova (gradivnih ...
... and this may also be the case with other proteins, including the gp210 and p62 nuclear pore proteins. Gp210 has increased ... Anti-centromere antibodies often correlate with developing portal hypertension.[31]. Anti-np62[32] and anti-sp100 are also ... The mechanism appears to be a cross reaction between the proteins of the bacterium and the mitochondrial proteins of the liver ... January 2007). "Anti-gp210 and Anti-Centromere Antibodies Are Different Risk Factors for the Progression of Primary Biliary ...
... proteins with central functions in meiosis are similar to key proteins in natural transformation in bacteria and DNA transfer ... still bound by centromeres. These centromeres would prevent their replication in subsequent division, resulting in four ... at which point linear chromosomes would be bound together with centromeres. The homologous nature of the two viral centromeres ... These proteins could have been transferred to the cell membrane during viral reproduction, enabling cell-to-cell fusion between ...
One such particular protein complex that is conserved between processes is RAD51, a well conserved recombinase protein that has ... the chromatids held together by the centromere are no longer identical. So, when the chromosomes go on to meiosis II and ... Mismatch repair (MMR) proteins, for instance, are a well-known regulatory family of proteins, responsible for regulating ... The MSH4 and MSH5 proteins form a hetero-oligomeric structure (heterodimer) in yeast and humans.[16][17][18] In the yeast ...
They are joined by a mid-way connection called a centromere.. (Cytokinesis takes place. The cell divides.). Telophase II. The ... DNA and associated chromatin protein. Genetically, the process is called recombination.. ... At this stage each chromosome is split into two sister chromatids, held together by the centromeres. The paired chromosomes now ...
In order for the Giemsa stain to adhere correctly, all chromosomal proteins must be digested and removed. For humans, white ... C-banding: Giemsa binds to constitutive heterochromatin, so it stains centromeres.The name is derived from centromeric or ... Attention is paid to their length, the position of the centromeres, banding pattern, any differences between the sex ... Silver staining: Silver nitrate stains the nucleolar organization region-associated protein. This yields a dark region where ...
Both structures serve to mediate binding to nuclear transport proteins.[5]. Most proteins, ribosomal subunits, and some DNAs ... and constitutive heterochromatin that consists of chromosome structural components such as telomeres and centromeres.[20] ... The nuclear lamina is composed mostly of lamin proteins. Like all proteins, lamins are synthesized in the cytoplasm and later ... Speckles are dynamic structures, and both their protein and RNA-protein components can cycle continuously between speckles and ...
Ro protein binds to Y RNA. Epidemiology[edit]. Approximately only 0.1 to 0.5 percent of the general population has the antibody ...
Interactions with proteins. All the functions of DNA depend on interactions with proteins. These protein interactions can be ... Telomeres and centromeres typically contain few genes but are important for the function and stability of chromosomes.[58][94] ... Structural proteins that bind DNA are well-understood examples of non-specific DNA-protein interactions. Within chromosomes, ... A distinct group of DNA-binding proteins is the DNA-binding proteins that specifically bind single-stranded DNA. In humans, ...
regulation of centromere complex assembly. • cell cycle arrest. • enucleate erythrocyte differentiation. • neuron ... The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that ... protein binding. • androgen receptor binding. • identical protein binding. • enzyme binding. • ubiquitin protein ligase binding ... protein localization to chromosome, centromeric region. • cell cycle. • striated muscle cell differentiation. • Ras protein ...
... and it involves a nuclear envelope protein Ran-GAP and the X-linked repeat array called Responder (Rsp), where the SD allele of ... "Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley ... which binds the growth protein and so slows growth, is maternally expressed (paternally silenced). The offspring is normal ...
The SBDS gene is expressed in all tissues and encodes a protein of 250 amino acid residues. The function of this protein is not ... this interval was refined to a region on the long arm of the chromosome next to the centromere.[12] ... The wide occurrence of the gene in all archaea and eukaryotes supports a role for this protein in a very fundamental and ... There is however, a great deal of indirect evidence to suggest that the SBDS protein may be involved in an aspect of cellular ...
PPP1R12A: protein fosphataza 1, regulatorni (inhibitor) subungfdit 12A. *PTPN11: protein tirozin fosphataza, ne-receptorski tip ... Prema položaju centromere, ubraja se u submetacentrike. Sadrži oko 143 miliona baznih parova (gradivnog materijala DNK), što ... heterohromatinski protein 1 CBX5: hromobox fhomolog 5. *COL2A1: kolagen, tip II, alfa 1 (primarni osteoartritis, ...
2002), I.3. Proteins: The Shape and Structure of Proteins *^ Alberts et al. (2002), I.3. Proteins: Protein Function Archived 25 ... like the fibers formed by the protein collagen. Proteins can bind to other proteins and simple molecules, sometimes acting as ... A single nucleotide difference within DNA can cause a change in the amino acid sequence of a protein. Because protein ... Protein structure is dynamic; the protein hemoglobin bends into slightly different forms as it facilitates the capture, ...
They have four pairs of telocentric chromosomes (whose centromeres sit at one of the telomeres), two to four pairs of ... "More DNA support for a Cetacea/Hippopotamidae clade: the blood-clotting protein gene gamma-fibrinogen" (PDF). Molecular ... The remaining chromosomes are metacentric-the centromere is approximately in the middle-and are rather small. Sperm whales, ...
The cladogram of external relationships, based on a 2008 DNA and protein analysis, shows the order as a clade, most closely ... reduced recombination rates and centromere mapping using half-tetrad analysis". Genetics. 167 (1): 243-252. doi:10.1534/ ... "The Hymenopteran Tree of Life: Evidence from Protein-Coding Genes and Objectively Aligned Ribosomal Data". PLOS ONE. 8 (8): ...
Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centromere-associated protein E is a ... Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of ... "Entrez Gene: CENPE centromere protein E, 312kDa". "OMIM Entry - # 616051 - MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; ... 1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5 ...
In humans, centromere protein B is encoded by the CENPB gene. Centromere protein B is a highly conserved protein that ... Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. ... Centromere protein B is a potential biomarker of small-cell lung cancer. Centromere GRCh38: Ensembl release 89: ENSG00000125817 ... "Entrez Gene: centromere protein B". Sugimoto K, Yata H, Himeno M (July 1993). "Mapping of the human CENP-B gene to chromosome ...
Most plant, animal and fungal centromeres also bind a large protein, centromere protein C (CENP-C), that is characterized by a ... Adaptive evolution of centromere proteins in plants and animals.. Talbert PB1, Bryson TD, Henikoff S. ... Portions of the human CENP-C protein implicated in centromere-targeting (purple bars) and DNA-binding (black bars) are shown at ... The rat CENP-A protein. (a) Alignment of predicted CENP-A proteins of mammals. Relative to other mammalian CENP-As, rat CENP-A ...
Centromere-associated protein-E (CENP-E; kinesin-7) is a kinetochore-associated kinesin motor protein with an essential and ... Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Kenneth W. Wood, Latesh Lad, Lusong Luo, ... Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Kenneth W. Wood, Latesh Lad, Lusong Luo, ... 2008) Centromere-associated protein E: A motor that puts the brakes on the mitotic checkpoint. Clin Cancer Res 14:7588-7592. ...
Compare centromere protein P ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, ... centromere protein P ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Your search returned 24 centromere protein P ELISA ELISA Kit across 3 suppliers. ...
... a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome ... May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (By ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10116. ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10116. ...
It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome ... Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as ... complex and may be involved in incorporation of newly synthesized CENPA into centromeres. ... a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The ...
J:57771 Sugata N, et al., Characterization of a novel kinetochore protein, CENP-H. J Biol Chem. 1999 Sep 24;274(39):27343-6 ...
A Synaptonemal Complex Protein Promotes Homology-Independent Centromere Coupling Message Subject. (Your Name) has forwarded a ... Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. We propose that centromere coupling ... During meiosis in wild-type diploids, centromere couples are initially nonhomologous and then undergo switching until all ... This transition to homologous coupling depends on Spo11, a protein required for the initiation of meiotic recombination. ...
A Synaptonemal Complex Protein Promotes Homology-Independent Centromere Coupling Message Subject. (Your Name) has forwarded a ...
... the centromere targeting region and the spindle targeting domain (Adams, 2001b). Inner centromere protein : Evolutionary ... Gene name - Inner centromere protein Synonyms - Cytological map position - 43A2--3 Function - signaling Keywords - chromatin ... Inner centromere protein : Biological Overview , Evolutionary Homologs , Regulation , Developmental Biology , Effects of ... EMS was chosen as a mutagen, with the aim of obtaining point mutations that specifically disrupt protein-protein interactions ( ...
CENP-B protein , centromere protein B , Major centromere autoantigen B , centromere autoantigen protein B , centromere protein ... Human Centromere Protein B (CENPB) Interaktionspartner * Given that CENP-B is the only centromere protein that binds centromere ... Centromere Protein B (CENPB) Antigen-Profil Protein Überblick This gene product is a highly conserved protein that facilitates ... anti-Centromere Protein B (CENPB) Antikörper. CENPB product is a highly conserved protein that facilitates centromere formation ...
... which is integrated into human DNA at the centromere on each chromosome, has a vital role in cell division. Work from Whitehead ... Faithful cell division requires tightly controlled protein placement at the centromeres Whitehead Institute for Biomedical ... Thin protein fibers, called microtubules stretch out from points on opposite sides of the cell and latch onto a protein complex ... Faithful cell division requires tightly controlled protein placement at the centromeres. Whitehead Institute for Biomedical ...
Evolution of Centromere/Kinetochore Proteins. All centromeres must perform the similar functions of connecting chromosomes to ... that the MIF2 gene of Saccharomyces cerevisiaeencodes a centromere protein with homology to the mammalian centromere protein ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ...
Protein level used designations for Centromere Protein B (CENPB) ELISA Kits CENP-B protein , centromere protein B , Major ... Additionally we are shipping Centromere Protein B Antibodies (41) and Centromere Protein B Proteins (7) and many more products ... Centromere Protein B (CENPB) ELISA Kits. CENPB product is a highly conserved protein that facilitates centromere formation. ... More ELISA Kits for Centromere Protein B Interaction Partners. Mouse (Murine) Centromere Protein B (CENPB) interaction partners ...
Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads.. Earnshaw WC1, ... This suggests that while CENP-C may be confined to the outer centromere in the kinetochore region, CENP-B may be distributed ... 2) CENP-B is present at both active and inactive centromeres of stable dicentric autosomes. CENP-C is not detectable at the ... We have screened for the presence of two centromere autoantigens, CENP-B (80 kDa) and CENP-C (140 kDa) at the inactive ...
Anchoring is mediated by RacA, which acts as a bridge between a centromere-like element in the vicinity of the origin of ... Defining a centromere-like element in Bacillus subtilis by Identifying the binding sites for the chromosome-anchoring protein ... Anchoring is mediated by RacA, which acts as a bridge between a centromere-like element in the vicinity of the origin of ... We propose that interactions between DNA bound RacA molecules cause the centromere-like element to fold up into a higher order ...
Centromere Protein O Antibody is available 2 times from supplier abbex at Gentaur.com shop ... Centromere Protein O Antibody. Centromere Protein O Antibody is available 2 times from Abbex labs abx111546 , Centromere ...
green fluorescent protein. INCENP. inner centromere protein. CENP-E. centromere protein E. ... 1997) Chromosomal proteins and cytokinesis: patterns of cleavage furrow formation and inner-centromere protein positioning in ... 1985) A family of centromere proteins is absent from the latent centromere of a stable isodicentric chromosome. Chromosoma ( ... A Dominant Mutant of Inner Centromere Protein (INCENP), a Chromosomal Protein, Disrupts Prometaphase Congression and ...
We identified a centromeric protein, Nuf2, which is conserved in fission yeast, human, nematode, and budding yeast. Gene ... The centromere is crucial for the proper segregation of chromosomes in all eukaryotic cells. ... A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link ... Interestingly, Nuf2 disappeared from the centromere during meiotic prophase when centromeres lose their connection to the ...
... interphase centromere complex protein 19; follicle-stimulating hormone primary response protein; CENP-I ... Recombinant Human CENPI Protein, His-tagged. E. coli. Human. His. +Inquiry. Cenpi-277M. Recombinant Mouse Cenpi Protein, His/ ... CENPI; centromere protein I; FSH primary response (LRPR1 homolog, rat) 1 , FSH primary response (LRPR1, rat) homolog 1 , ... Protein Function. CENPI has several biochemical functions, for example, protein binding. Some of the functions are cooperated ...
Products > Epigenetics Proteins > Cell Cycle Proteins > Cell division Proteins > Centromere Proteins Centromere Proteins NDE1 ... centromere protein A; CENP-A; CENPA; centromere protein A, 17kDa; centromere protein A (17kD); OTTHUMP00000122589; Centromere ... Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of ... Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of ...
Centromere protein F (Cenp-F) is a kinetochore protein required for chromosome segregation (Bomont et al., 2005; Holt et al., ... 2005). Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F. EMBO J. 24, 3927-3939 ... Protein farnesylation is typically involved in targeting proteins to membranes and, as far as we are aware, farnesylation has ... Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F ...
Distribution of centromere histone H3 over the 180 bp clusters. The HTR12 protein is a centromere-specific histone H3 variant ... In Z. mays, the CENP-A homologue CENH3 protein was suggested to form a complex with a centromere-specific repetitive sequence ... In the region encompassing the centromere, various kinds of proteins assemble to build up a kinetochore complex together with ... In yeasts and mammals, the interactions among centromere DNA and proteins have been extensively studied. The critical role of ...
Anti-Centromere Antibodies are useful as controls in ANA diagnostics and also react with other species including mouse, rat, ... Purified IgG derived from Human anti-Centromere positive serum (also known as CREST patient serum) conjugated to fluorescein ... Anti-Centromere Antibodies are useful as controls in ANA diagnostics and also react with other species including mouse, rat, ... Serum obtained from an autoimmune patient was purified by protein A chromatography and then tested at a series of dilutions by ...
... a polyclonal antibody designed to detect protein products of the gene CENPF by western blot and ELISA. ... Datasheet for Goat Anti-Centromere protein F / mitosin Antibody (EB05622) - ... CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/ ... Target Protein. Principal Names: CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... White boxes represent UTRs (untranslated regions). Orange: protein coding regions. The black lines connecting boxes represent ...
... thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein, Plant Molecular Biology" on ... Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein. Moraes, Izabel ... The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold ... Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein. Recognition of ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): Q9H5G1 - Homo sapiens 0 * Q2KIW5 - Bos taurus no matching PDB entries ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ...
A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones DK Palmer, DK Palmer ... DK Palmer, K ODay, MH Wener, BS Andrews, RL Margolis; A 17-kD centromere protein (CENP-A) copurifies with nucleosome core ... We have detected and begun to characterize a 17-kD centromere-specific protein, CENP-A (Earnshaw, W. C., and N. Rothfield, 1985 ... We conclude that this centromere-specific protein is a histone-like component of chromatin. The data suggest that CENP-A ...
  • We describe a process in meiotic cells of budding yeast in which chromosomes become joined together in pairs at their centromeres independent of chromosomal homology. (sciencemag.org)
  • Critical to successful cell division is the integrity of the centromere--a region of DNA on each chromosome where the cell division machinery attaches to segregate the chromosomes. (eurekalert.org)
  • As mitosis progresses, the microtubules align the chromosomes along the mid-line of the cell, then shorten and pull the chromosome pairs at their centromeres to opposite sides of the cell. (eurekalert.org)
  • If the chromosomes have too many or too few centromeres, or the centromeres are located in the wrong place, proper chromosome segregation fails, and the cell either dies or becomes diseased. (eurekalert.org)
  • E ukaryotic organisms show wide diversity in the manner by which they organize the centromere/kinetochore of their chromosomes. (rupress.org)
  • The chromosomes of the budding yeast Saccharomyces cerevisiae have a well-defined point centromere: all centromeric functions are restricted to a short stretch of DNA ∼125 bp in length (for review see Clarke, 1990 ). (rupress.org)
  • Mammalian centromeres, which contain hundreds of kilobases of repetitive, heterochromatic, α-satellite DNA, are found at the major constriction of mitotic chromosomes where they specify a trilaminar kinetochore. (rupress.org)
  • Propose that CENP-B plays a dual role in centromere formation, ensuring de novo formation on DNA lacking a functional centromere but preventing the formation of excess centromeres on chromosomes. (antibodies-online.com)
  • 123:373-385), is shown here to bind chromosomes, but is unable to target to the centromere. (rupress.org)
  • Thus, association with the chromosomes, and specifically with centromeres, appears to be essential for INCENP targeting to the correct spindle subdomain at anaphase. (rupress.org)
  • Passengers are tightly associated with chromosomes in the early stages of mitosis, and many of these proteins transiently concentrate at centromeres during metaphase ( Earnshaw and Mackay, 1994 ). (rupress.org)
  • We have suggested that passenger proteins might be mitotic cytoskeletal proteins whose association with chromosomes during prometaphase and metaphase targets them to the sites where they act during anaphase and telophase ( Earnshaw and Bernat, 1990 ). (rupress.org)
  • The centromere is crucial for the proper segregation of chromosomes in all eukaryotic cells. (nih.gov)
  • Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. (creativebiomart.net)
  • The centromere of eukaryotic chromosomes is an important structure for precise chromatid segregation during mitotic and meiotic cell divisions. (biologists.org)
  • To investigate the structural requirements for centromere targeting, constructs for EYFP-tagged CENH3 of A. lyrata, A. arenosa, Capsella bursa-pastoris, Zea mays and Luzula nivea (the latter with holocentric chromosomes) were transformed into A. thaliana. (deepdyve.com)
  • Chromosomes containing two centromeres are known as dicentric and often mis-segregate during cell division, resulting in aneuploidy or chromosome breakage. (springer.com)
  • Dicentric chromosome can be stabilized by centromere inactivation, a process which reestablishes monocentric chromosomes. (springer.com)
  • Centromere positions on the majority of orthologous chromosomes are different in these two species. (springer.com)
  • The inner centromere proteins (INCENPs), [5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. (wikipedia.org)
  • Centromeres are specialised regions of the genome, which can be identified under the microscope as the primary constriction in X-shaped chromosomes. (redorbit.com)
  • The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the centromeres. (redorbit.com)
  • It helps regulate gene activity (expression) by modifying chromatin, the complex of DNA and protein that packages DNA into chromosomes. (medlineplus.gov)
  • Centromeres of most organisms are embedded within constitutive heterochromatin, the condensed regions of chromosomes that account for a large fraction of complex genomes. (sdbonline.org)
  • It's name stems from the observation that these proteins colocalise on condensing chromosomes during prophase, and are carried along to centromeres and to the equator of the mitotic spindle during metaphase. (sdbonline.org)
  • Anaphase centromeres that join the sister chromatids split sister chromatids separate becoming individual chromosomes. (prezi.com)
  • If there are 20 centromeres in a cell at anaphase, how many chromosomes are there in each daughter cell following cytokinesis? (google.com)
  • The centromeres are specialized loci on chromosomes that form primary constrictions during mitosis. (biomedcentral.com)
  • Once per life cycle, mitotic nuclear divisions are replaced by meiosis I and II - reducing chromosome number from the diploid level to a haploid genome, reshuffling the homologous chromosomes by their centromeres, and recombining chromosome arms by crossing-over. (worldcat.org)
  • Centromeres are regions of chromosomes that direct formation of the kinetochore and its subsequent attachment to the spindle, enabling the faithful segregation of the genetic material during cell division. (asm.org)
  • Telomeres are specialized protein-DNA constructs present at the ends of eukaryotic chromosomes, which prevent them from degradation and end-to-end chromosomal fusion. (wikipedia.org)
  • In addition to ParABS systems, most bacteria harbor condensin complexes, members of the structural maintenance of chromosomes (SMCs) family of proteins found in all kingdoms of life 23 . (nature.com)
  • May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. (proteopedia.org)
  • The data obtained demonstrate that the rye and wheat chromosomes studied are involved in genetic regulation of centromere division in meiotic anaphase I (AI). (deepdyve.com)
  • Stable maize ( Zea mays ) chromosomes were recovered from an unstable dicentric containing large and small versions of the B chromosome centromere. (plantcell.org)
  • However, some of the 180 bp repeat clusters, particularly those that were long or stretched at interphase, were not fully covered with the signals from anti-HTR12 or AtCENP-C. Chromatin fiber immunolabeling clearly revealed that the centromeric proteins examined in this study, localize only at the knobs on the extended chromatin fibers, which form a limited part of the 180 bp clusters. (biologists.org)
  • We conclude that this centromere-specific protein is a histone-like component of chromatin. (rupress.org)
  • The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. (nih.gov)
  • Here we describe the proteome of Drosophila melanogaster centromeres as analyzed by quantitative affinity purification-mass spectrometry (AP-MS). The AP-MS input chromatin material was prepared from D. melanogaster cell lines expressing CENP-ACID or H3.3 fused to EGFP as baits. (mendeley.com)
  • Centromere chromatin enriched proteins were identified based on their relative abundance in CENP-ACID-GFP compared to H3.3-GFP or mock affinity-purifications. (mendeley.com)
  • The analysis yielded 86 proteins specifically enriched in centromere chromatin preparations.The data accompanying the manuscript on this approach (Barth et al. (mendeley.com)
  • The HAC have been used successfully to understand the chromatin organization and epigenetic status of human centromeres [ 1 - 3 ], and the structure of the kinetochore [ 4 ]. (pubmedcentralcanada.ca)
  • Multifunctional Sin3 proteins bind histone deacetylases (HDACs) to assemble silencing complexes that selectively target chromatin. (nature.com)
  • Vertebrate centromeres are epigenetically defined by nucleosomes containing the histone H3 variant, CENP-A. CENP-A nucleosome assembly requires the three-protein Mis18 complex (Mis18α, Mis18 , and M18BP1) that recruits the CENP-A chaperone HJURP to centromeres, but how the Mis18 complex recognizes centromeric chromatin is unknown. (bireme.br)
  • The large size of regional centromeres may be important for the additional functions that have been attributed to centromeres of higher eukaryotes, including chromosome adhesion in achiasmate disjunction ( 32 ), as well as providing domains of specialized chromatin structure (heterochromatin) in which euchromatic gene transcription and recombination are both repressed. (asm.org)
  • The overall bending angle is 59 degrees, which may be important for the centromere-specific chromatin structure. (proteopedia.org)
  • To explain these remarkable evolutionary features for a single-copy gene that is needed at every mitosis, we propose that CENP-Cs, like some CenH3s, suppress meiotic drive of centromeres during female meiosis. (nih.gov)
  • While some insects like Drosophila also have a regional centromere/kinetochore ( Goldstein, 1981 ), other insects and some nematodes, including Caenorhabditis elegans , organize holokinetochores with continuous trilaminar plates along the entire length of the chromosome that are visible during mitosis ( Albertson and Thomson, 1982 ). (rupress.org)
  • These experiments provide evidence of an unexpected link between this chromosomal protein and cytokinesis, and suggest that one function of INCENP may be to integrate the chromosomal and cytoskeletal events of mitosis. (rupress.org)
  • The present study involved the use of several INCENP truncation mutants to further analyze the function of this protein in mitosis. (rupress.org)
  • Centromere protein-F (CENP-F) is a large nuclear protein of 367 kDa, which is involved in multiple mitosis-related events such as proper assembly of the kinetochores, stabilization of heterochromatin, chromosome alignment and mitotic checkpoint signaling. (forskningsdatabasen.dk)
  • CENP-E is a kinesin-like motor protein that localizes to the kinetochore during mitosis and is essential for bipolar spindle formation. (medkoo.com)
  • In the present study, we used a yeast two-hybrid screen and identified a novel interaction between DNMT3B and constitutive centromere protein CENP-C. CENP-C is itself essential for mitosis. (oup.com)
  • Having one and only one centromere per chromosome is essential for proper chromosome segregation during both mitosis and meiosis. (springer.com)
  • Centromeres are the chromosomal regions responsible for poleward movement at meiosis and mitosis, and are essential for the faithful segregation of genetic information. (sdbonline.org)
  • During mitosis the centromeres can be seen during the metaphase stage as a constriction at the chromosome. (wikipedia.org)
  • Sixty-four were designated as "core" centromere/kinetochore components based on peak expression and/or well-characterized functions during mitosis. (biomedcentral.com)
  • Transcriptional co-expression and PPI network analyses with known human centromere/kinetochore proteins as a query group help identify novel potential mitosis regulators. (biomedcentral.com)
  • However, the use of inter-chromosomal circularization engenders chromosome instability by creating two centromeres in a single chromosome, causing chromosomal breakage during mitosis. (wikipedia.org)
  • The centromere is the region of a chromosome that organizes the kinetochore for chromosome movement during mitosis and meiosis. (plantcell.org)
  • First, the centromere nondisjoins at the second pollen mitosis, which produces the two maize sperm. (plantcell.org)
  • This study shows that ICP0 induces the proteasomal-dependent degradation of the centromeric protein CENP-B in infected as well as ICP0-expressing cells. (antibodies-online.com)
  • To assess the centromere activities of the 180 bp repeats, we performed indirect fluorescence immunolabeling with antibodies against phosphorylated histone H3 at Ser10, HTR12 ( Arabidopsis centromeric histone H3 variant) and AtCENP-C ( Arabidopsis CENP-C homologue) for the A. thaliana cell cultures. (biologists.org)
  • In the region encompassing the centromere, various kinds of proteins assemble to build up a kinetochore complex together with species-specific centromeric DNA. (biologists.org)
  • Therefore, antibodies raised against these proteins, should serve as good markers for identifying the active centromeres as well as for detecting centromeric and phosphorylated histone H3 in A. thaliana . (biologists.org)
  • CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM;117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al. (creative-biogene.com)
  • Centromere protein A (Cenpa for mouse, CENP-A for other species) is a histone H3-like protein that is thought to be involved in the nucleosomal packaging of centromeric DNA. (bris.ac.uk)
  • Centromeric proteins include chromosomal passenger complex. (sdbonline.org)
  • In higher eukaryotes, however, centromeric regions of the genome not only are much larger (up to 5 Mb in length [see reference 55 for review]) but show no apparent sequence conservation and have been referred to as "regional" centromeres. (asm.org)
  • e.g. they appear only on active centromeres and not on inactive ones ( Choo, 1997 ). (biologists.org)
  • All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). (nih.gov)
  • Indirect evidence from humans suggests that megabase arrays of α-satellite or alphoid DNAs, which are a family of A+T-rich 171-bp tandem repeats, are associated with active centromeres ( 46 ) and may be sufficient for centromere function ( 23 , 68 ). (asm.org)
  • This inactive centromere can be inherited from one generation to the next attached to the active version and loses all known cytological and molecular properties of active centromeres. (plantcell.org)
  • Although they perform a conserved function in chromosome segregation, centromeres are typically composed of repetitive satellite sequences that are rapidly evolving. (nih.gov)
  • Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. (uniprot.org)
  • Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. (uniprot.org)
  • These results indicate that at least some aspects of the functional role of the ZW10 protein in ensuring proper chromosome segregation are conserved across large evolutionary distances. (rupress.org)
  • We propose that Nuf2 acts at the centromere to establish a connection with the spindle for proper chromosome segregation, and that Nuf2 function is also required for the spindle checkpoint. (nih.gov)
  • Schubert, Ingo 2010-12-29 00:00:00 The centromere is an essential chromosomal component assembling the kinetochore for chromosome attachment to the spindle microtubules and for directing the chromosome segregation during nuclear division. (deepdyve.com)
  • We identified a novel kinetochore protein family conserved from yeast to human that is essential for equal chromosome segregation. (rupress.org)
  • The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. (nih.gov)
  • Under electron microscope centromeres and kinetochores are contiguous structurally, and both play important roles in regulating chromosome segregation. (biomedcentral.com)
  • Centromere-associated protein-E (CENP-E) is a kinetochore-associated mitotic kinesin that is thought to function as the key receptor responsible for mitotic checkpoint signal transduction after interaction with spindle microtubules. (pnas.org)
  • kinesin-7) is a kinetochore-associated kinesin motor protein with an essential and exclusive role in metaphase chromosome alignment and satisfaction of the mitotic checkpoint ( 1 ). (pnas.org)
  • The C-terminal, noncatalytic region of CENP-E is not only sufficient to specify localization to kinetochores, but it also mediates interaction of CENP-E with the serine/threonine kinase BubR1, a key effector of mitotic checkpoint signaling that forms complexes with the checkpoint proteins Cdc20, Bub3, and Mad2 to inhibit the ubiquitin ligase activity of the anaphase promoting complex APC/C CDC20 ( 4 - 7 ). (pnas.org)
  • The centromere is the default chromosomal region onto which the mitotic/meiotic kinetochore gradually assembles to ensure correct chromosome attachment to microtubules and equal segregation of sister chromatids ( Perpelescu & Fukagawa, 2011 ). (peerj.com)
  • Spindle checkpoint protein hMad2 temporally localizes at kinetochores at early mitotic stages after RNAi. (rupress.org)
  • The RNAi deficiency of CENP-A leads to a similar mitotic phenotype, but the kinetochore signals of other kinetochore proteins, hMis6 and CENP-C, are greatly diminished. (rupress.org)
  • while TRIP13 is a novel kinetochore protein and directly interacts with mitotic checkpoint silencing protein p31 comet . (biomedcentral.com)
  • A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. (abcam.com)
  • INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes. (portlandpress.com)
  • May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (By similarity). (uniprot.org)
  • Deposition of new CENPA-containing nucleosomes at the centromere. (uniprot.org)
  • The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. (uniprot.org)
  • Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. (uniprot.org)
  • Centromere protein A (CENPA) was one of the first identified kinetochore components in humans. (peerj.com)
  • [8] The constitutive proteins include CENPA (centromere protein A), CENPB , CENPC1 , and CENPD . (wikipedia.org)
  • Immunofluorescence analysis of interphase cells at day 5.5 reveals complete Cenpa depletion, diffuse Cenpb foci, absence of discrete Cenpc signal on centromeres, and dispersion of Cenpb and Cenpc throughout the nucleus. (bris.ac.uk)
  • CENPB product is a highly conserved protein that facilitates centromere formation. (antikoerper-online.de)
  • Budding yeast have short and simple centromeres, the minimum required size of which is only ∼125 bp, while higher eukaryotes have much larger and/or more complex centromeres ( Choo, 1997 ). (biologists.org)
  • The composition of kinetochore proteins and their cellular roles are, however, poorly understood in higher eukaryotes. (rupress.org)
  • Centromeres of higher eukaryotes are epigenetically defined by the centromere specific histone H3 variant CENP-ACID. (mendeley.com)
  • The identification of a CENP-B-related centromere DNA-binding protein in S. pombe strongly supports the hypothesis that fission yeast centromeres are structurally and functionally related to the centromeres of higher eukaryotes. (singerinstruments.com)
  • Abstract Background Centromeres represent the last frontiers of plant and animal genomics. (journaldatabase.info)
  • Centromere regions contain distinct epigenetic marks, including dense DNA hypermethylation, yet the mechanisms by which DNA methylation is targeted to these regions remains largely unknown. (oup.com)
  • A particularly promising candidate for this kind of epigenetic centromere marking is a variant form of the H3 histone known as CenH3. (redorbit.com)
  • The evidence is consistent with the proposal of a critical epigenetic function for CENP-A in marking a chromosomal region for centromere formation. (bris.ac.uk)
  • H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. (abcam.com)
  • This study defines cellular mechanisms for recruiting CENP-A assembly factors to existing CENP-A nucleosomes for the epigenetic inheritance of centromeres. (bireme.br)
  • Centromeres are specified and maintained by sequence-independent epigenetic mechanisms through the incorporation of CENP-A into centromeres. (bireme.br)
  • Several regional centromeres display an epigenetic control phenomenon called centromere activation. (asm.org)
  • These findings indicate an epigenetic component to centromere specification in that centromere inactivation can be directed by joining two centromeres in opposition. (plantcell.org)
  • Antibodies against the human ZW10 protein display a cell cycle-dependent staining pattern in HeLa cells strikingly similar to that previously observed for DmZW10 in dividing Drosophila cells. (rupress.org)
  • Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
  • Additionally we are shipping Centromere Protein B Antibodies (41) and Centromere Protein B Proteins (7) and many more products for this protein. (antibodies-online.com)
  • We have screened for the presence of two centromere autoantigens, CENP-B (80 kDa) and CENP-C (140 kDa) at the inactive centromere of a naturally occurring stable dicentric chromosome using specific antibodies that do not cross-react with any other chromosomal proteins. (nih.gov)
  • In order to discriminate between the active and inactive centromeres on this chromosome we have developed a modification of the standard methanol/acetic acid fixation procedure that allows us to obtain high-quality cytological spreads that retain antigenicity with the anti-centromere antibodies. (nih.gov)
  • 3) While immunofluorescence with anti-CENP-C antibodies typically gives two discrete spots, staining with anti-CENP-B often appears as a single bright bar connecting both sister centromeres. (nih.gov)
  • Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. (creativebiomart.net)
  • Anti-Centromere Antibodies are useful as controls in ANA diagnostics and also react with other species including mouse, rat, and hamster. (antibodiesinc.com)
  • After transformation, immunostaining of root tip nuclei with anti-GFP antibodies yielded only diffuse signals, indicating that the original three amino acids are necessary but not sufficient for targeting A. thaliana centromeres. (deepdyve.com)
  • We have affinity purified the anti-17-kD centromere protein (anti-CENP-A) specific antibodies from immunoblots of HeLa nuclear protein. (rupress.org)
  • We can assist you in finding the right ELISA kits, PCR kits, recombinant proteins, antibodies, provide you with datasheets, manuals, kit protocols and other information about the products. (gentaurshop.com)
  • In normal individuals, the immune system produces antibodies to foreign proteins ( antigens ) but not to human proteins ( autoantigens ). (wikipedia.org)
  • There are many subtypes of ANAs such as anti-Ro antibodies , anti-La antibodies , anti-Sm antibodies , anti-nRNP antibodies , anti-Scl-70 antibodies , anti-dsDNA antibodies , anti-histone antibodies , antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies . (wikipedia.org)
  • These cells coordinate an immune response upon the detection of foreign proteins ( antigens ), producing antibodies that bind to these antigens. (wikipedia.org)
  • [7] [14] The ENAs consist of ribonucleoproteins and non- histone proteins, named by either the name of the donor who provided the prototype serum (Sm, Ro, La, Jo), or the name of the disease setting in which the antibodies were found (SS-A, SS-B, Scl-70). (wikipedia.org)
  • CENP-A, -B, and -C are major centromere components and the main targets of anticentromere antibodies (ACA). (fujita-hu.ac.jp)
  • Western blotting and an ELISA with bacterially expressed recombinant CENP-O protein were performed to screen for the presence of anti-CENP-O antibodies. (fujita-hu.ac.jp)
  • In plants we find that CENP-C proteins have complex duplicated regions, with conserved amino and carboxyl termini that are dissimilar in sequence to their counterparts in animals and fungi. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. (ctdbase.org)
  • Centromere location in Arabidopsis is unaltered by extreme divergence in CENH3 protein sequence. (pacb.com)
  • In most organisms the position of the centromere is not determined by the DNA sequence. (redorbit.com)
  • In most organisms the position of the centromere is not determined by the sequence of the DNA building blocks, i.e. the DNA sequence, but epigenetically. (redorbit.com)
  • The only exception to this rule is the unicellular fungus baker's yeast, in which a specific DNA sequence "encodes" the position of the centromere. (redorbit.com)
  • Histone proteins bind the DNA largely independently of the underlying sequence and help to package the long thread-like DNA molecule. (redorbit.com)
  • An effective representation of a protein sequence plays a crucial role in protein sub-nuclear localization. (mdpi.com)
  • The existing representations, such as dipeptide composition (DipC), pseudo-amino acid composition (PseAAC) and position specific scoring matrix (PSSM), are insufficient to represent protein sequence due to their single perspectives. (mdpi.com)
  • The core problem of protein sub-nuclear localization using machine learning method includes two aspects: constructing good representations for collecting as much protein sequence information as possible, and developing effective models for prediction. (mdpi.com)
  • Another complexity of centromere mapping is that sequence alone cannot be used to accurately predict where centromeres begin and end. (genetics.org)
  • Updated DNA Sequences ===================== YSCCHROMI L22015 U00091 41988bp linear PLN 29-JAN-2004 Saccharomyces cerevisiae chromosome I centromere and right arm sequence. (bio.net)
  • One protein, identified by both approaches, shows significant homology to the human centromere DNA-binding protein, CENP-B, and is identical to Abp1p (autonomously replicating sequence-binding protein 1) (Murakami, Y., J.A. Huberman, and J. Hurwitz. (singerinstruments.com)
  • The centromeres, formed on a repetitive sequence called minor satellite [ 12 ], localize at the periphery of the chromocenters [ 11 ]. (pubmedcentralcanada.ca)
  • It has three components: ParM, an actin-like protein that forms a long filament to push two plasmids apart, ParR, which binds the plasmid to ParM and generates the ParM filament, and parC, which is a DNA sequence on the plasmid that anchors ParR to itself. (wikipedia.org)
  • The characteristics and arrangement of these elements are similar to those seen in the Drosophila centromere, but the relative abundance of each class of repeats, as well as the sequence degeneracy of the transposon-like elements, is unique to Neurospora . (asm.org)
  • The overall size and sequence complexity of centromeres generally appears to parallel the developmental complexity of the organism. (asm.org)
  • Despite recent advances in the development of higher eukaryotic experimental systems, relatively little is known about the sequence constituents of centromeres and centric heterochromatin in complex organisms. (asm.org)
  • The sequence components necessary for full centromere function appear to include transposable elements of several types as well as low-complexity satellite DNAs ( 67 ). (asm.org)
  • The apparent absence of a defined sequence that is responsible for kinetochore formation, as is seen in S. cerevisiae , suggests a redundant, nonsequence-specific initiation event leading to centromere and/or kinetochore formation. (asm.org)
  • Subtelomeres are considered to be the most distal (farthest from the centromere) region of unique DNA on a chromosome, and they are unusually dynamic and variable mosaics of multichromosomal blocks of sequence. (wikipedia.org)
  • The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box), which appears in every other alpha-satellite repeat. (proteopedia.org)
  • Centromere protein B (CENP-B) binds to this DNA region and is involved in the assembly of the kinetochore. (surmodics.com)
  • Each of these antibody subtypes binds to different proteins or protein complexes within the nucleus. (wikipedia.org)
  • mRNA levels of CENP-A and CENP-B, a centromere protein that binds independently of CENP-A, were measured in breast cancer specimens from 484 patients receiving no systemic therapy, 276 patients receiving tamoxifen, and 233 patients treated with neoadjuvant chemotherapy. (biomedcentral.com)
  • P P RB protein RB protein E2F E2F E2F CDK not present E2F not released released E2F E2F binds to DNA. (slideshare.net)
  • P P P P breakdown of p53 no DNA damage DNA damage phosphorylated p53 DNA repair proteins apoptosis p53 binds to DNA. (slideshare.net)
  • Finally, higher plants have yet different regional centromeres that organize kinetochores in a ball and cup-type fashion ( Bajer and Mole-Bajer, 1969 ). (rupress.org)
  • Moreover, kinetochores consist of more than 16 different proteins. (peerj.com)
  • For information on the structure and constituents of centromeres and kinetochores, see Organization of the animal kinetochore in The dynamic kinetochore-microtubule interface . (sdbonline.org)
  • This SPB-centromere proximity requires microtubules and functional kinetochores, which are protein complexes formed on the centromeres and capable of binding microtubules. (duhnnae.com)
  • Furthermore, human cells show localization of anti-CENP-A antibody to centromeres by immunofluorescence microscopy, whereas rat cells do not. (rupress.org)
  • The numerical experiments on two public datasets with KNN classifier and cross-validation tests showed that in terms of the common indexes of sensitivity, specificity, accuracy and MCC, the proposed fusing representations outperform the traditional representations in protein sub-nuclear localization, and the representation treated by LDA outperforms the untreated one. (mdpi.com)
  • Thus, nuclear protein plays a very important role in the research on disease prevention and clinical medicine where the correct protein sub-nuclear localization is essential. (mdpi.com)
  • Researchers in the past two decades have made great progress in the study of protein representation methods and sub-cellular localization prediction [ 2 ]. (mdpi.com)
  • However, the task, with a large number of protein sequences having been generated, requires us to find faster localization methods. (mdpi.com)
  • An attractive route in recent studies is to utilize machine learning for protein sub-nuclear localization [ 4 ]. (mdpi.com)
  • Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (ox.ac.uk)
  • To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice. (ox.ac.uk)
  • An extensive literature review has led to a compilation of 196 human centromere/kinetochore proteins, all with experimental evidence supporting the subcellular localization. (biomedcentral.com)
  • Here, we showed that centromere localization of the Mis18 complex depends on CENP-A, but not CENP-C or CENP-T, in chicken DT40 cells. (bireme.br)
  • Furthermore, we demonstrated that M18BP1/KNL2, a member of the Mis18 complex, contained the CENP-C-like motif in chicken and other vertebrates, which is essential for centromere localization and M18BP1/KNL2 function in DT40 cells. (bireme.br)
  • Even among fungi, the difference in functional centromere size is considerable. (rupress.org)
  • Second, CENP-C CR rigidifies the N-terminal tail of H4 in the conformation favoring H4 K20 monomethylation, essential for a functional centromere. (rcsb.org)
  • INCENP is a tightly bound chromosomal protein that transfers to the spindle midzone at the metaphase/anaphase transition. (rupress.org)
  • An INCENP truncation mutant (INCENP 1-405 ) that targets to centromeres but lacks the microtubule association region acquires strong dominant-negative characteristics. (rupress.org)
  • INCENP 1-405 apparently exerts its effect by displacing the endogenous protein from centromeres. (rupress.org)
  • Inner centromere protein is a protein that in humans is encoded by the INCENP gene . (wikipedia.org)
  • INCENP is a regulatory protein in the chromosome passenger complex . (wikipedia.org)
  • 31 Inner Centromere Protein Antigens 135/155kDa (INCENP) Antikörper von 8 Herstellern verfügbar auf www.antikoerper-online.de. (antikoerper-online.de)
  • Your search returned 24 centromere protein P ELISA ELISA Kit across 3 suppliers. (biocompare.com)
  • Product Description:Bovine Centromere protein U, MLF1IP ELISA KIT allows for the in vitro quantitative determination of Bovine Centromere protein U, MLF1IP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. (lifescience-market.com)
  • You need info about Human Centromere Protein(CENP-B) ELISA Kit or any other Gentaur produtct? (gentaurshop.com)
  • DmZW10 displays an intriguing cell cycle-dependent intracellular distribution, apparently moving from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase, and back to the centromere/kinetochore at anaphase (Williams, B.C., M. Gatti, and M.L. Goldberg. (rupress.org)
  • In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. (nih.gov)
  • RESULTS: In human cells, Survivin appeared first on centromeres bound to a novel para-polar axis during prophase/metaphase, relocated to the spindle midzone during anaphase/telophase, and disappeared at the end of telophase. (ox.ac.uk)
  • We found that in G1 and in late anaphase, SPB-centromere proximity was disturbed in mutant cells lacking Ctf19 complex members,Chl4p and-or Ctf19p, whose centromeres lay further away from their SPBs than those of the wild-type cells. (duhnnae.com)
  • The reactivated centromere regains the molecular attributes of activity in anaphase I of meiosis. (plantcell.org)
  • Centromere_CenpF_leu-rich_rpt. (genome.jp)
  • The syndrome is caused by mutations in both copies of the CENPF gene, which codes for centromere protein F. This protein is involved in cell division, in which it forms part of a disc-shaped protein complex known as a kinetochore. (wikipedia.org)
  • It discusses current knowledge of the biological roles of CENP-S and CENP-X and how their dual existence may be a common feature of CCAN (constitutive centromere-associated network) proteins. (nih.gov)
  • Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant CENP-A, upon which the constitutive centromere-associated network of proteins (CCAN) is built. (rcsb.org)
  • Interestingly, Nuf2 disappeared from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body. (nih.gov)
  • Centromere function requires the coordination of many processes including kinetochore assembly, sister chromatid cohesion, spindle attachment and chromosome movement. (sdbonline.org)
  • In the budding yeast, centromeres stay clustered near the spindle pole bodies SPBs through most of the cell cycle. (duhnnae.com)
  • We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. (mcponline.org)
  • Thin protein fibers, called microtubules stretch out from points on opposite sides of the cell and latch onto a protein complex, called the kinetochore, which is anchored by CENP-A molecules at the centromere. (eurekalert.org)
  • In animal cells, Taxol disrupts microtubule formation by binding to microtubules and accelerating their assembly from the protein precursor, tubulin. (google.com)
  • Only those wells that contain Bovine Centromere protein U, MLF1IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (lifescience-market.com)
  • Here we review the current literature on centromere protein F, its association with breast cancer and present the first case of this antibody being identified in a person with a BRCA1 gene mutation. (beds.ac.uk)
  • In addition, anti-CENP-O antibody-positive sera were tested by Western blotting HeLa cell extracts to examine reactivity with the major centromere antigens. (fujita-hu.ac.jp)
  • The nucleosomes of centromeres are characterized by a special H3-like histone (CenH3), which evolves rapidly and adaptively in Drosophila and Arabidopsis. (nih.gov)
  • In this paper we describe the molecular evolution of one centromere/kinetochore component, originally identified as the protein product of the Drosophila melanogaster gene l(1)zw10 , hereafter called DmZW10. (rupress.org)
  • In contrast to mammalian systems, the protein composition of Drosophila centromeres has not been comprehensively investigated. (mendeley.com)
  • These results suggest that the Neurospora centromere is heterochromatic and regional in character, more similar to centromeres of Drosophila than to those of most single-cell yeasts. (asm.org)
  • For Drosophila , the centromere of a minichromosome has been mapped by deletion analysis, and the minimal sequences required for function are now being identified ( 37 , 67 ). (asm.org)
  • CENPP (Centromere Protein P) is a Protein Coding gene. (genecards.org)
  • During meiosis in wild-type diploids, centromere couples are initially nonhomologous and then undergo switching until all couples involve homologs. (sciencemag.org)
  • Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. (sciencemag.org)
  • This transition to homologous coupling depends on Spo11, a protein required for the initiation of meiotic recombination. (sciencemag.org)
  • FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers. (nih.gov)
  • Whereas we find no evidence that mammalian CenH3 (CENP-A) has been evolving adaptively, mammalian CENP-C proteins contain adaptively evolving regions that overlap with regions of DNA-binding activity. (nih.gov)
  • In mammalian cells, two broad groups of centromere -interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins. (wikipedia.org)
  • BACKGROUND: Survivin is a mammalian protein that carries a motif typical of the inhibitor of apoptosis (IAP)proteins, first identified in baculoviruses. (ox.ac.uk)
  • Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates. (bireme.br)
  • Based on these results, we conclude that M18BP1/KNL2 is essential for centromere formation through direct binding to CENP-A nucleosome in non-mammalian vertebrates. (bireme.br)
  • Since the nucleus is the largest cell organelle guiding the process of biological cell life, researchers have focused on seeking out the location(s) in the nucleus of the query protein so as to explore its function. (mdpi.com)
  • Previously it has been shown that the absence of non-essential kinetochore proteins of the Ctf19 complex weakens kinetochore-microtubule interaction, but whether this compromised interaction affects centromere-kinetochore positioning inside the nucleus is unknown. (duhnnae.com)
  • Using a combination of fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on metaphase chromosome spreads, we demonstrate that centromere inactivation has evolved on a neo-Y chromosome fusion in the Japan Sea threespine stickleback fish ( Gasterosteus nipponicus ). (springer.com)
  • Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. (genetics.org)
  • In response to this, the chromosome could induce centromere inactivation to impede the formation of two centromeres, but this would induce heterochromatin formation in centromeres. (wikipedia.org)
  • Sera from several humans with CREST scleroderma autoimmune disease (CREST: calcinosis, Raynaud's phenomenon, esophageal dsymotility, sclerodactyly, and telangiectasia) bind this protein in immunoblot assays of HeLa whole cell or nuclear extracts. (rupress.org)
  • Only human nuclear protein is CENP-A positive by immunoblot. (rupress.org)
  • The protein is a component of the nuclear matrix during the G2 phase of interphase. (nih.gov)
  • It is well known that if proteins are wrongly located or are largely accumulated in improper parts in nuclear, genetic diseases, and even cancer, will be caused [ 1 ]. (mdpi.com)
  • Antinuclear autoantibody immunofluorescence performed for workup of possible inflammatory arthropathy showed a high titre cell cycle related nuclear speckled pattern, with subsequent confirmation by addressable laser bead immunoassay of the target antigen as an immunodominant epitope at the C-terminus of centromere protein F. (beds.ac.uk)
  • Many other proteins are also assembled around CENP-A nucleosomes in interphase nuclei to form the interphase centromere complex (ICEN). (fujita-hu.ac.jp)
  • Each time the cell makes a copy of its DNA in preparation for cell division, it needs to ensure that the centromeres of the new and old DNA strand get re-filled with CENP-A. Although scientists have known that the centromere replenishes its stock of CENP-A deposits during a phase of the cell cycle referred to as G1, exactly how this process is regulated had remained a mystery. (eurekalert.org)
  • A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle. (nih.gov)
  • The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle . (wikipedia.org)
  • The CENP-O protein is a component of the ICEN that localizes at the centromere throughout the cell cycle. (fujita-hu.ac.jp)
  • DNA cell cycle proteins phosphorylated RB CDK present b. (slideshare.net)
  • Using Xenopus egg extract, we show that direct, cell-cycle-regulated binding of M18BP1 to CENP-A nucleosomes recruits the Mis18 complex to interphase centromeres to promote new CENP-A nucleosome assembly. (bireme.br)
  • Genetic and biochemical strategies have been used to identify Schizosaccharomyces pombe proteins with roles in centromere function. (singerinstruments.com)
  • CENH3 has a variable N-terminal and a more conserved C-terminal part, including the loop1 region of the histone fold domain, which is considered to be critical for centromere targeting. (deepdyve.com)
  • Except for LnCENH3, all recombinant CENH3 proteins targeted A. thaliana centromeres, but the more distantly related the heterologous protein is, the lower is the efficiency of targeting. (deepdyve.com)
  • Scientists from the Max Planck Institute of Immunobiology and Epigenetics in Freiburg have succeeded in demonstrating that the position, function and inheritance of the centromere are determined by the histone CenH3, a DNA packaging protein. (redorbit.com)
  • CenH3 arises exclusively in DNA regions at the centromere in various organisms. (redorbit.com)
  • Patrick Heun's research group at the Max Planck Institute of Immunobiology and Epigenetics and colleagues from the Helmholtz Research Center in Munich have now discovered that CenH3 alone is sufficient to trigger the formation of the centromere. (redorbit.com)
  • For their experiments, the researchers equipped the CenH3 histone with an artificially attached DNA binding domain so that the protein could bind to a DNA region where a centromere does not normally form. (redorbit.com)
  • The protein is able to recruit additional CenH3 proteins independently. (redorbit.com)
  • This ensures that sufficient CenH3 is available at the centromere after each cell division. (redorbit.com)
  • Otherwise, the available CenH3 proteins would be reduced by half after each cell division. (redorbit.com)
  • The insights into the central role of CenH3 for centromere identity could also prove important for medicine. (redorbit.com)
  • Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8. (genetics.org)
  • In normal physiology, lymphocytes that recognise human proteins ( autoantigens ) either undergo programmed cell death ( apoptosis ) or become non-functional. (wikipedia.org)
  • Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. (nih.gov)
  • Autoantibodies directed to centromere protein F were first reported in 1993 and their association with malignancy has been well documented. (beds.ac.uk)
  • Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity. (nature.com)
  • Both studies indicated that centromere repeats of 400-500 kb in length are involved in maintaining their functions, although the centromere organization and DNA sequences are very different in the two organisms. (biologists.org)
  • These sequences bind specific proteins called "cen"-proteins. (wikipedia.org)
  • These findings also indicate a structural aspect to centromere specification revealed by the gain of activity at the site of the previously inactive sequences. (plantcell.org)
  • Many of these proteins which help the kinetochore associate with DNA are conserved among eukaryotic species ( Perpelescu & Fukagawa, 2011 ). (peerj.com)
  • The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance. (ctdbase.org)
  • Here, we investigate the role of DNA-partitioning protein ParB and SMC condensin complexes in the actinobacterium Corynebacterium glutamicum . (nature.com)
  • In Saccharomyces cerevisiae , for example, the centromere is small, consisting of only approximately 200 bp for full function. (asm.org)
  • We propose that centromere coupling facilitates homolog pairing and promotes synapsis initiation. (sciencemag.org)
  • Although baculoviral IAP proteins regulate cell death, the yeast Survivin homolog Bir1 is involved in cell division. (ox.ac.uk)
  • Complex II contains a nonessential Sin3 homolog, Pst2, and several conserved proteins. (nature.com)
  • Purified IgG derived from Human anti-Centromere positive serum (also known as CREST patient serum) conjugated to fluorescein isothiocyanate. (antibodiesinc.com)
  • Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy. (semanticscholar.org)
  • Fingerprint Dive into the research topics of 'A helix-turn-helix structure unit in human centromere protein B (CENP-B)'. Together they form a unique fingerprint. (uthscsa.edu)
  • Human Centromeres Produce Chromosome-Specific and Array-Specific Alpha Satellite Transcripts that Are Complexed with CENP-A and CENP-C. (bireme.br)
  • Centromere activity can be observed, however, in activated human neocentromeres lacking alphoid repeats ( 19 ). (asm.org)
  • Our data further suggest that there have been genetic changes to this centromere in the two million years since the formation of the neo-Y chromosome, but it remains unclear whether these genetic changes are a cause or consequence of centromere inactivation. (springer.com)
  • It preferentially targets transcribed chromosomal regions and centromere cores. (nature.com)
  • THE centromeres in most plants and animals contain hundreds of kilobases of simple repeats and interspersed retroelements. (genetics.org)
  • Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. (nih.gov)
  • Owens TW, Gilmore AP, Streuli CH, Foster FM (2013) Inhibitor of Apoptosis Proteins: Promising Targets for Cancer Therapy. (omicsonline.org)
  • The Inhibitor of Apoptosis (IAP) proteins act downstream of a broad range of stimuli, such as cytokines and extracellular matrix interactions, to regulate cell survival, proliferation and migration. (omicsonline.org)
  • Since their discovery almost 20 years ago, the Inhibitor of Apoptosis (IAP) family of proteins have gathered growing interest as possible drug targets in a wide range of malignancies. (omicsonline.org)
  • The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Centromere protein U, MLF1IP in the samples is then determined by comparing the O.D. of the samples to the standard curve. (lifescience-market.com)
  • By its involvement in controlling the activity of other genes, this protein plays an important role in the development and function of many parts of the body. (medlineplus.gov)
  • By interrogating and integrating online resources, we have mined for genes/proteins that display transcriptional co-expression or PPI with the core centromere/kinetochore components. (biomedcentral.com)
  • Here, we performed a small-scale zebrafish screening for these genes and discovered a new therapeutic target, centromere protein X (CENPX), which was further validated in a T2DM mouse model. (figshare.com)
  • Since the nucleolus is a major site of RNA processing and ribonucleoprotein assembly, nucleoli are targeted by viruses, directly when viral RNA and proteins enter the nucleolus and indirectly when viruses induce increased expression of cellular polyamine genes. (frontiersin.org)
  • Although subtelomeres are pseudogenes and do not code for protein, they provide an evolutionary advantage by diversifying genes. (wikipedia.org)
  • 2) CENP-B is present at both active and inactive centromeres of stable dicentric autosomes. (nih.gov)
  • CENP-C is not detectable at the inactive centromeres. (nih.gov)
  • When two copies of the dicentric chromosome with one active and one inactive centromere are present, homologous chromosome pairing reduces the frequency of intrachromosomal recombination and thus decreases, but does not eliminate, the reactivation of inactive centromeres. (plantcell.org)
  • The staining pattern obtained was consistent with the pattern expected for anti-centromere staining. (antibodiesinc.com)