Centromere Protein B: A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance.Centromere: The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.Chromosomal Proteins, Non-Histone: Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.Autoantigens: Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.Kinetochores: Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.Pulmonary Surfactant-Associated Protein B: A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.Chromosome Segregation: The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.Mitosis: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.Aurora Kinase B: An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. It mediates proper CHROMOSOME SEGREGATION and contractile ring function during CYTOKINESIS.Aurora Kinases: A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. They are involved in many aspects of cell division, including centrosome duplication, SPINDLE APPARATUS formation, chromosome alignment, attachment to the spindle, checkpoint activation, and CYTOKINESIS.DNA, Satellite: Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.Chromosomes: In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Transferrin-Binding Protein B: A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.Synaptonemal Complex: The three-part structure of ribbon-like proteinaceous material that serves to align and join the paired homologous CHROMOSOMES. It is formed during the ZYGOTENE STAGE of the first meiotic division. It is a prerequisite for CROSSING OVER.Meiotic Prophase I: The prophase of the first division of MEIOSIS (in which homologous CHROMOSOME SEGREGATION occurs). It is divided into five stages: leptonema, zygonema, PACHYNEMA, diplonema, and diakinesis.Chromosome Pairing: The alignment of CHROMOSOMES at homologous sequences.Meiosis: A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.Spermatocytes: Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.Crossing Over, Genetic: The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes.Mitosis Modulators: Agents that affect MITOSIS of CELLS.Schizosaccharomyces: A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.Schizosaccharomyces pombe Proteins: Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Chromosomes, Plant: Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Epididymal Secretory Proteins: Proteins secreted by the epididymal epithelium. These proteins are both tissue- and species-specific. They are important molecular agents in the process of sperm maturation.Ovarian Neoplasms: Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.CA-125 Antigen: Carbohydrate antigen most commonly seen in tumors of the ovary and occasionally seen in breast, kidney, and gastrointestinal tract tumors and normal tissue. CA 125 is clearly tumor-associated but not tumor-specific.Neoplasms, Glandular and Epithelial: Neoplasms composed of glandular tissue, an aggregation of epithelial cells that elaborate secretions, and of any type of epithelium itself. The concept does not refer to neoplasms located in the various glands or in epithelial tissue.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Hypopharyngeal Neoplasms: Tumors or cancer of the HYPOPHARYNX.Hypopharynx: The bottom portion of the pharynx situated below the OROPHARYNX and posterior to the LARYNX. The hypopharynx communicates with the larynx through the laryngeal inlet, and is also called laryngopharynx.Carcinoma, Squamous Cell: A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)Pharyngectomy: Surgical removal of a part of the pharynx. (Dorland, 28th ed)H-Reflex: A monosynaptic reflex elicited by stimulating a nerve, particularly the tibial nerve, with an electric shock.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Prometaphase: The phase of cell nucleus division following PROPHASE, when the breakdown of the NUCLEAR ENVELOPE occurs and the MITOTIC SPINDLE APPARATUS enters the nuclear region and attaches to the KINETOCHORES.Coturnix: A genus of BIRDS in the family Phasianidae, order GALLIFORMES, containing the common European and other Old World QUAIL.Chromosomes, Artificial, Mammalian: DNA constructs that are composed of, at least, all elements, such as a REPLICATION ORIGIN; TELOMERE; and CENTROMERE, that are required for successful replication, propagation to and maintainance in progeny mammalian cells. In addition, they are constructed to carry other sequences for analysis or gene transfer.Quail: Common name for two distinct groups of BIRDS in the order GALLIFORMES: the New World or American quails of the family Odontophoridae and the Old World quails in the genus COTURNIX, family Phasianidae.Chromosomes, Artificial, Human: DNA constructs that are composed of, at least, all elements, such as a REPLICATION ORIGIN; TELOMERE; and CENTROMERE, required for successful replication, propagation to and maintainance in progeny human cells. In addition, they are constructed to carry other sequences for analysis or gene transfer.DNA Packaging: The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.

Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. (1/99)

Human centromeres contain large arrays of alpha-satellite DNA that are thought to provide centromere function. The arrays show size and sequence variation, but the extent to which extremely low levels of this DNA can occur on normal centromeres is unclear. Using a set of chromosome-specific alpha-satellite probes for each of the human chromosomes, we performed interphase fluorescence in situ hybridization (FISH) in a population-screening study. Our results demonstrate that extreme reduction of chromosome-specific alpha satellite is unusually common in chromosome 21 (screened with the alphaRI probe), with a prevalence of 3.70%, compared to < or =0.12% for each of chromosomes 13 and 17, and 0% for the other chromosomes. No analphoid centromere was identified in >17,000 morphologically normal chromosomes studied. All of the low-alphoid centromeres are fully functional as indicated by their mitotic stability and binding to centromere proteins CENP-B, CENP-C, and CENP-E. Sensitive metaphase FISH analysis of the low-alphoid chromosome 21 centromeres established the presence of residual alphaRI as well as other non-alphaRI alpha-satellite DNA suggesting that centromere function may be provided by (1) the residual alphaRI DNA, (2) other non-alphaRI alpha-satellite sequences, (3) a combination of 1 and 2, or (4) an activated neocentromere DNA. The low-alphoid centromeres, in particular those of chromosome 21, should provide unique opportunities for the study of the evolution and the minimal DNA requirement of the human centromere.  (+info)

Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. (2/99)

Centromere protein B (CENP-B) binds constitutively to mammalian centromere repeat DNA and is highly conserved between humans and mouse. Cenpb null mice appear normal but have lower body and testis weights. We demonstrate here that testis-weight reduction is seen in male null mice generated on three different genetic backgrounds (denoted R1, W9.5, and C57), whereas body-weight reduction is dependent on the genetic background as well as the gender of the animals. In addition, Cenpb null females show 31%, 33%, and 44% reduced uterine weights on the R1, W9.5, and C57 backgrounds, respectively. Production of "revertant" mice lacking the targeted frameshift mutation but not the other components of the targeting construct corrected these differences, indicating that the observed phenotype is attributable to Cenpb gene disruption rather than a neighbouring gene effect induced by the targeting construct. The R1 and W9.5 Cenpb null females are reproductively competent but show age-dependent reproductive deterioration leading to a complete breakdown at or before 9 months of age. Reproductive dysfunction is much more severe in the C57 background as Cenpb null females are totally incompetent or are capable of producing no more than one litter. These results implicate a further genetic modifier effect on female reproductive performance. Histology of the uterus reveals normal myometrium and endometrium but grossly disrupted luminal and glandular epithelium. Tissue in situ hybridization demonstrates high Cenpb expression in the uterine epithelium of wild-type animals. This study details the first significant phenotype of Cenpb gene disruption and suggests an important role of Cenpb in uterine morphogenesis and function that may have direct implications for human reproductive pathology.  (+info)

Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. (3/99)

Centromere protein A (Cenpa for mouse, CENP-A for other species) is a histone H3-like protein that is thought to be involved in the nucleosomal packaging of centromeric DNA. Using gene targeting, we have disrupted the mouse Cenpa gene and demonstrated that the gene is essential. Heterozygous mice are healthy and fertile whereas null mutants fail to survive beyond 6.5 days postconception. Affected embryos show severe mitotic problems, including micronuclei and macronuclei formation, nuclear bridging and blebbing, and chromatin fragmentation and hypercondensation. Immunofluorescence analysis of interphase cells at day 5.5 reveals complete Cenpa depletion, diffuse Cenpb foci, absence of discrete Cenpc signal on centromeres, and dispersion of Cenpb and Cenpc throughout the nucleus. These results suggest that Cenpa is essential for kinetochore targeting of Cenpc and plays an early role in organizing centromeric chromatin at interphase. The evidence is consistent with the proposal of a critical epigenetic function for CENP-A in marking a chromosomal region for centromere formation.  (+info)

Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. (4/99)

Two functionally important DNA sequence elements in centromeres of the fission yeast Schizosaccharomyces pombe are the centromeric central core and the K-type repeat. Both of these DNA elements show internal functional redundancy that is not correlated with a conserved DNA sequence. Specific, but degenerate, sequences in these elements are bound in vitro by the S. pombe DNA-binding proteins Abp1p (also called Cbp1p) and Cbhp, which are related to the mammalian centromere DNA-binding protein CENP-B. In this study, we determined that Abp1p binds to at least one of its target sequences within S. pombe centromere II central core (cc2) DNA with an affinity (K(s) = 7 x 10(9) M(-1)) higher than those of other known centromere DNA-binding proteins for their cognate targets. In vivo, epitope-tagged Cbhp associated with centromeric K repeat chromatin, as well as with noncentromeric regions. Like abp1(+)/cbp1(+), we found that cbh(+) is not essential in fission yeast, but a strain carrying deletions of both genes (Deltaabp1 Deltacbh) is extremely compromised in growth rate and morphology and missegregates chromosomes at very high frequency. The synergism between the two null mutations suggests that these proteins perform redundant functions in S. pombe chromosome segregation. In vitro assays with cell extracts with these proteins depleted allowed the specific assignments of several binding sites for them within cc2 and the K-type repeat. Redundancy observed at the centromere DNA level appears to be reflected at the protein level, as no single member of the CENP-B-related protein family is essential for proper chromosome segregation in fission yeast. The relevance of these findings to mammalian centromeres is discussed.  (+info)

Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (5/99)

BACKGROUND: Survivin is a mammalian protein that carries a motif typical of the inhibitor of apoptosis (IAP)proteins, first identified in baculoviruses. Although baculoviral IAP proteins regulate cell death, the yeast Survivin homolog Bir1 is involved in cell division. To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice. RESULTS: In human cells, Survivin appeared first on centromeres bound to a novel para-polar axis during prophase/metaphase, relocated to the spindle midzone during anaphase/telophase, and disappeared at the end of telophase. In the mouse, Survivin was required for mitosis during development. Null embryos showed disrupted microtubule formation, became polyploid, and failed to survive beyond 4.5days post coitum. This phenotype, and the cell-cycle localization of Survivin, resembled closely those of INCENP. Because the yeast homolog of INCENP, Sli15, regulates the Aurora kinase homolog Ipl1p, and the yeast Survivin homolog Bir1 binds to Ndc10p, a substrate of Ipl1p, yeast Survivin, INCENP and Aurora homologs function in concert during cell division. CONCLUSIONS: In vertebrates, Survivin and INCENP have related roles in mitosis, coordinating events such as microtubule organization, cleavage-furrow formation and cytokinesis. Like their yeast homologs Bir1 and Sli15, they may also act together with the Aurora kinase.  (+info)

Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. (6/99)

Three human centromere proteins, CENP-A, CENP-B and CENP-C, are a set of autoantigens specifically recognized by anticentromere antibodies often produced by patients with scleroderma. Microscopic observation has indicated that CENP-A and CENP-C localize to the inner plate of metaphase kinetochore, while CENP-B localizes to the centromere heterochromatin beneath the kinetochore. The antigenic structure, called "prekinetochore", is also present in interphase nuclei, but little is known about its molecular organization and the relative position of these antigens. Here, to visualize prekinetochore in living cells, we first obtained a stable human cell line, MDA-AF8-A2, in which human CENP-A is exogenously expressed as a fusion to a green fluorescent protein of Aequorea victoria. Simultaneous staining with anti-CENP-B and anti-CENP-C antibodies showed that the recombinant CENP-A colocalized with the endogenous CENP-C and constituted small discrete dots attaching to larger amorphous mass of CENP-B heterochromatin. When the cell growth was arrested in G1/ S phase with hydroxyurea, CENP-B heterochromatin was sometimes highly extended, while the relative location between GFP-fused CENP-A and the endogenous CENP-C was not affected. These results indicated that the fluorescent CENP-A faithfully localizes to the centromere/kinetochore throughout the cell cycle. We then obtained several mammalian cell lines where the same GFP-fused human CENP-A construct was stably expressed and their centromere/kinetochore is fluorescent throughout the cell cycle. These cell lines will further be used for visualizing the prekinetochore locus in interphase nuclei as well as analyzing kinetochore dynamics in the living cells.  (+info)

Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. (7/99)

Several members of protein families that are conserved in higher eukaryotes are known to play a role in centromere function in the fission yeast Schizosaccharomyces pombe, including two homologs of the mammalian centromere protein CENP-B, Abp1p and Cbh1p. Here we characterize a third S. pombe CENP-B homolog, Cbh2p (CENP-B homolog 2). cbh2Delta strains exhibited a modest elevation in minichromosome loss, similar to cbh1Delta or abp1Delta strains. cbh2Delta cbh1Delta strains showed little difference in growth or minichromosome loss rate when compared to single deletion strains. In contrast, cbh2Delta abp1Delta strains displayed dramatic morphological and chromosome segregation defects, as well as enhancement of the slow-growth phenotype of abp1Delta strains, indicating partial functional redundancy between these proteins. Both cbh2Delta abp1Delta and cbh1Delta abp1Delta strains also showed strongly enhanced sensitivity to a microtubule-destabilizing drug, consistent with a mitotic function for these proteins. Cbh2p was localized to the central core and core-associated repeat regions of centromeric heterochromatin, but not at several other centromeric and arm locations tested. Thus, like its mammalian counterpart, Cbh2p appeared to be localized exclusively to a portion of centromeric heterochromatin. In contrast, Abp1p was detected in both centromeric heterochromatin and in chromatin at two of three replication origins tested. Cbh2p and Abp1p homodimerized in the budding yeast two-hybrid assay, but did not interact with each other. These results suggest that indirect cooperation between different CENP-B-like DNA binding proteins with partially overlapping chromatin distributions helps to establish a functional centromere.  (+info)

Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. (8/99)

The mechanisms that specify precisely where mammalian kinetochores form within arrays of centromeric heterochromatin remain largely unknown. Localization of CENP-A exclusively beneath kinetochore plates suggests that this distinctive histone might direct kinetochore formation by altering the structure of heterochromatin within a sub-region of the centromere. To test this hypothesis, we experimentally mistargeted CENP-A to non-centromeric regions of chromatin and determined whether other centromere-kinetochore components were recruited. CENP-A-containing non-centromeric chromatin assembles a subset of centromere-kinetochore components, including CENP-C, hSMC1, and HZwint-1 by a mechanism that requires the unique CENP-A N-terminal tail. The sequence-specific DNA-binding protein CENP-B and the microtubule-associated proteins CENP-E and HZW10 were not recruited, and neocentromeric activity was not detected. Experimental mistargeting of CENP-A to inactive centromeres or to acentric double-minute chromosomes was also not sufficient to assemble complete kinetochore activity. The recruitment of centromere-kinetochore proteins to chromatin appears to be a unique function of CENP-A, as the mistargeting of other components was not sufficient for assembly of the same complex. Our results indicate at least two distinct steps in kinetochore assembly: (1) precise targeting of CENP-A, which is sufficient to assemble components of a centromere-prekinetochore scaffold; and (2) targeting of kinetochore microtubule-associated proteins by an additional mechanism present only at active centromeres.  (+info)

We report the interaction between a human centromere antigen and an alphoid DNA, a human centromeric satellite DNA, which consists of 170-bp repeating units. A cloned alphoid DNA fragment incubated with a HeLa cell nuclear extract is selectively immunoprecipitated by the anticentromere sera from scleroderma patients. Immunoprecipitation of the DNA made by primer extension defines the 17-bp segment on the alphoid DNA that is required for formation of DNA-antigen complex. On the other hand, when proteins bound to the biotinylated alphoid DNA carrying the 17-bp motif are recovered by streptavidin agarose and immunoblotted, the 80-kD centromere antigen (CENP-B) is detected. DNA binding experiments for proteins immunoprecipitated with anticentromere serum, separated by gel electrophoresis, and transferred to a membrane strongly suggest that the 80-kD antigen specifically binds to the DNA fragment with the 17-bp motif. The 17-bp motif is termed the "CENP-B box." Alphoid monomers with the CENP-B box ...
The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box), which appears in every other alpha-satellite repeat. In the present study, the crystal structure of the complex of the DNA-binding region (129 residues) of CENP-B and the CENP-B box DNA has been determined at 2.5 A resolution. The DNA-binding region forms two helix-turn-helix domains, which are bound to adjacent major grooves of the DNA. The DNA is kinked at the two recognition helix contact sites, and the DNA region between the kinks is straight. Among the major groove protein-bound DNAs, this kink-straight-kink bend contrasts with ordinary round bends (gradual bending between two protein contact sites). The larger kink (43 degrees ) is induced by a novel mechanism, phosphate bridging by an arginine-rich helix: the recognition helix with an arginine cluster is inserted perpendicularly into the major groove and bridges the groove through direct interactions with ...
Reactivity: Chicken, Cow, Dog and more. Compare 39 different CENPB ELISA Kits & buy the right one directly at antibodies-online.com!
A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance ...
Two ZMPSTE24 mutations in the yeast to complement the (S. cerevisiae) mating defect STE24 and Ras-converting enzyme 1 (RCE1; another prenylprotein-specific endoprotease) genes [§§] (farnesylated protein-converting enzymes 1 and 2) is a significant component of the rice centromere antigens lamin A/C and B1 identified (in non-transgenic plants that go awry↩ (centromere) in cancer), the Ras…
In a subset of patients with limited cutaneous (lc) systemic sclerosis (SSc), anti-CENP-A antibodies (Ab) cross-react with a peptide (FOXE3p53-62) that presents striking homology with one of the two immunodominant epitopes of CENP-A (Ap17-30). We searched for clinical correlates of anti-FOXE3p53-62 Ab by measuring their levels along with those of Ab to Ap17-30 and to the second immunodominant epitope of CENP-A, namely Ap1-17. Serum samples were obtained from 121 patients with SSc, 46 patients with systemic lupus erythematosus (SLE) and 25 healthy blood donors (HBD). The reactivity of serum IgG to Ap1-17, Ap17-30 and FOXE3p53-62 was measured by ELISA. The corresponding anti-peptide Ab were affinity-purified from pooled SSc sera and used to establish standard curves for quantifying these Ab in patients and HBD. Receiver operating characteristics (ROC) analysis, comparing SSc patients who were positive for anti-CENP Ab (ACA+) to those who were negative, was used to find cut-off points for dichotomizing the
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Find MSDS or SDS, a COA, data sheets and more information.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Mouse monoclonal antibody raised against a full length recombinant CENPA. CENPA (AAH00881, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00001058-M01) - Products - Abnova
A process for preparing a polarizer is described whereby a pre-polarizing article comprising an oriented, vinylalcohol polymer film layer, and an acid donor layer comprising a thermal acid generator, is exposed to radiant energy at a temperature sufficient to effect a partial dehydration of the vinylalcohol polymer to a vinylalcohol/poly(acetylene) copolymer.
CENPA antibody [3-19] (centromere protein A) for ICC/IF, IHC, IHC-P, Neut, WB. Anti-CENPA mAb (GTX13939) is tested in Human, Chicken samples. 100% Ab-Assurance.
CENPT antibody (centromere protein T) for ELISA, ICC/IF, IHC-F, IHC-P, IP, WB. Anti-CENPT pAb (GTX51767) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Gene target information for CENPV - centromere protein V (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Does anyone here have a set of these? http://www.ptreeusa.com/panel_glue_up.htm My wife gifted me two sets when they were on a 40% off sale. I havent used them yet but have mounted them to a 2x attached too a wall and together theyll give about 7 of clamping length. They claim to work from thin to up to 5 thick. When I first saw them I immediately thought of a benchtop to break them in. |g| Id planned on adding a Bessey K-type between each of these clamps, plus one or two on each end.
Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few centromere proteins are conserved in all of these four organisms and many are unique to the different organisms. The recent analysis of the centromere structure in the yeast S. pombe by electron microscopy and detailed immunofluorescence microscopy of ...
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008 ...
We found that the CENP‐A NAC/CAD network plays an important role in regulating MT-kinetochore attachment and promotes efficient chromosome alignment on the metaphase plate, but is dispensable for MT-attachment per se. Our data indicate that CENP‐A NAC/CAD is not required for loading of the KMN network and that it regulates chromosome congression independently of the MIND complex (see Figure 8B). It is important to note that these findings contradict previous models in which CENP‐A NAC/CAD is essential for the loading of the KMN subcomplexes MIND and NDC80 onto kinetochores (Liu et al, 2006; Okada et al, 2006; Kwon et al, 2007). At the functional level, such a linear model of kinetochore assembly is inconsistent with both our own and other CENP‐A NAC/CAD knockdown phenotypes; while NDC80 depletion results in MT attachment failure, loss of CENP‐H, CENP‐I, Chl4R, CENP‐U or CENP‐T allows the formation of a partial metaphase plate, which requires stable attachment and ...
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. To examine the molecular basis for the specification of centromeric chromatin, we have cloned a human cDNA that encodes the 17-kD histone-like centromere antigen, CENP-A. Two domains are evident in the 140 aa CENP-A polypeptide: a unique NH2-terminal domain and a 93-amino acid COOH-terminal domain that shares 62% identity with nucleosomal core protein, histone H3. An epitope tagged derivative of CENP-A was faithfully targeted to centromeres when expressed in a variety of animal cells and this targeting activity was shown to reside in the histone-like COOH-terminal domain of CENP-A. These data clearly indicate that the assembly of centromeres is driven, at least in part, by the incorporation of a novel core histone into centromeric chromatin. ...
Accurate chromosome segregation requires the assembly of kinetochores, multiprotein complexes that assemble on the centromere of each sister chromatid. A key step in this process involves binding of the constitutive centromere‐associated network (CCAN) to CENP‐A, the histone H3 variant that constitutes centromeric nucleosomes. This network is proposed to operate as a persistent structural scaffold for assembly of the outer kinetochore during mitosis. Here, we show by fluorescence resonance energy transfer (FRET) that the N‐terminus of CENP‐N lies in close proximity to the N‐terminus of CENP‐A in vivo, consistent with in vitro data showing direct binding of CENP‐N to CENP‐A. Furthermore, we demonstrate in living cells that CENP‐N is bound to kinetochores during S phase and G2, but is largely absent from kinetochores during mitosis and G1. By measuring the dynamics of kinetochore binding, we reveal that CENP‐N undergoes rapid exchange in G1 until the middle of S phase when it ...
UA62784 is a specific inhibitor of centromere protein E (CENPE) kinesin-like protein; mitotic inhibitor. CENP-E is a kinesin-like motor protein that localizes to the kinetochore during mitosis and is essential for bipolar spindle formation. UA62784 is a novel specific inhibitor of CENP-E, which likely binds within the motor domain of CENP-E. UA62784 causes reversible cell cycle arrest in mitosis before metaphase, which leads to apoptosis. The compound is not interacting with microtubules directly. (Last Updated: 6/22/2015)
It has recently been proposed that mitotic chromosomes transport certain cytoskeletal proteins to the metaphase plate so that these proteins are able to subsequently participate in the assembly of the anaphase spindle and the cleavage furrow. To understand how such proteins accomplish their dual chromosomal: cytoskeletal role, we have begun a molecular and functional analysis of the inner centromere proteins (INCENPs), founder members of the class of "chromosome passenger proteins". cDNA clones encoding the open reading frames of the two chicken INCENPs were recovered. The predicted proteins, class I INCENP (96,357 D) and class II INCENP (100,931 D) are novel, and differ from each other by the inclusion of a 38-codon insert within the class II coding region. Transient expression of the chicken INCENPs in mammalian cells confirms that the signals and structures required for the transfer of these proteins from chromosomes to cytoskeleton are evolutionarily conserved. Furthermore, these studies ...
1989) and may interact with tubulin (Balczon and Brinkley, 1987). Simerly et al. (1990) suggested that the CENP-B protein could be involved in either microtubule assembly at the kinetochore or in sliding of the kinetochore along the microtubules. ,1990). Injection of these purified IgGs into tissue culture cells disrupts microtubule attachment to kinetochores, chromosome congression, and chromosome segregation, depending on the stage of the cell cycle at the time of injection. The authors suggest that most of their results can be explained by defects in the assembly or maturation of the kinetochores such that either microtubules do not attach to the kinetochores or the attachment is defective. Other investigators have also reported the presence of Ca2-binding, contractile proteins in the centrosome, but have suggested other functions for the proteins. Baron et al. (1991) have suggested that the Ca2-binding, centrosomal proteins in marsupial and mammalian cell lines, identified by antibodies ...
CENPP is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM;117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006;(PubMed 16622420 ...
The histone protein CenH3 is both necessary and sufficient to trigger the formation of centromeres and pass them on from 1 generation to the next. Centromeres are specialised regions of the genome, which can be identified under the microscope as the primary constriction in X-shaped chromosomes. The cell skeleton, which distributes the chromosomes to the two daughter cells during cell division, attaches to the centromeres. In most organisms the position of the centromere is not determined by the DNA sequence. Scientists from the Max Planck Institute of Immunobiology and Epigenetics in Freiburg have succeeded in demonstrating that the position, function and inheritance of the centromere are determined by the histone CenH3, a DNA packaging protein. This discovery may help to further the development of artificial human chromosomes, which could be used for gene therapies in medicine.. Centromeres provide a platform for the development of a protein complex known as the kinetochore. During cell ...
DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation ...
Academic Journals Database is a universal index of periodical literature covering basic research from all fields of knowledge, and is particularly strong in medical research, humanities and social sciences. Full-text from most of the articles is available. Academic Journals Database contains complete bibliographic citations, precise indexing, and informative abstracts for papers from a wide range of periodicals.
Replication of alpha-satellite DNA arrays in endogenous human centromeric regions and in human artificial chromosome Indri Erliandri, Haiqing Fu, Megumi Nakano, Jung-Hyun Kim, Karen H. Miga,Mikhail Liskovykh, William C. Earnshaw, Hiroshi Masumoto, Natalay Kouprina, Mirit I. Aladjem, and Vladimir Larionov Nucl. Acids Res. 2014 42: 11502-11516 ...
rs438034 is a SNP in the centromere protein F CENPF gene. Based on a long-term (up to 15 years) study of 749 Swedish women with breast cancer, carriers of rs438034(T) alleles (as oriented wrt dbSNP) had poorer specific survival rates as compared to rs438034(C;C) individuals. The hazard ratio was 2.65 (CI: 1.19-5.90). However, although rs438034(T) carriers had worse survival odds, they were less likely to have either regional lymph node metastases (odds ratio 0.71, CI: 0.51-1.01) or tumors of stage II-IV (OR 0.73, CI 0.54-0.99). [PMID 19008095] ...
Centromere B Ab ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of IgG class autoantibodies against Centromere B in human serum or plasma. (KA1275) - Products - Abnova
The Bioconductor-mirror/GWASTools package contains the following man pages: alleleFrequency allequal anomDetectBAF anomDetectLOH anomIdentifyLowQuality anomSegStats apartSnpSelection assocCoxPH assocRegression BAFfromClusterMeans BAFfromGenotypes batchTest centromeres chromIntensityPlot convertNcdfGds createDataFile defunct duplicateDiscordance duplicateDiscordanceAcrossDatasets duplicateDiscordanceProbability exactHWE findBAFvariance GdsGenotypeReader-class GdsIntensityReader-class GdsReader-class gdsSubset genoClusterPlot GenotypeData-class genotypeToCharacter getobj getVariable GWASTools-package hetByScanChrom hetBySnpSex HLA ibdPlot imputedDosageFile IntensityData-class intensityOutliersPlot manhattanPlot MatrixGenotypeReader-class meanIntensityByScanChrom mendelErr mendelList missingGenotypeByScanChrom missingGenotypeBySnpSex NcdfGenotypeReader-class NcdfIntensityReader-class NcdfReader-class pasteSorted pcaSnpFilters pedigreeCheck pedigreeDeleteDuplicates pedigreeMaxUnrelated
Author Summary The centromere is a chromosome domain essential for the correct partitioning of chromosomes during mitotic and meiotic cell divisions. The characterization of the centromeric proteins and their sequential assembly have been extensively studied in mammalian mitosis, since defective chromosome segregation is associated with birth defects and cancer. However, few studies have analyzed the centromere assembly during meiosis, a special cell division leading to the production of haploid gametes. Here, we analyze the sequence of loading of several centromeric and kinetochoric proteins during male mouse meiosis. We show that during both meiotic divisions, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The outer kinetochore proteins BubR1 and CENP-E are the last ones to be assembled. We also demonstrate, using a knockout mouse for Sgol2, that the inner centromeric protein Shugoshin 2 is
Centromeres are unique chromatin domains that direct the site of kinetochore formation during mitosis and mediate the movement of chromosomes during cell division. Centromeres contain a unique nucleosome in which histone H3 is replaced by centromere protein A (CENP-A). Because of their unique position in chromatin, the CENP-A nucleosome was hypothesized to determine the site of centromere and kinetochore assembly. In order to test the long held assumption that CENP-A dictates the location of the centromere; we developed a novel de novo centromere formation assay, which provides a new and powerful constructive approach to studying centromeres. This system is based on a LacO array that is stably integrated into the long arm of chromosome 1, far away from the existing centromere. Targeting the CENP-A chaperone HJURP or the Mis18 complex to the LacO array, by fusing them to the LacI repressor, drove the stable recruitment of CENP-A nucleosomes to the LacO array at the non-centromeric locus. ...
DNA methylation is an epigenetically imposed mark of transcriptional repression that is essential for maintenance of chromatin structure and genomic stability. Genome-wide methylation patterns are mediated by the combined action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B. Compelling links exist between DNMT3B and chromosome stability as emphasized by the mitotic defects that are a hallmark of ICF syndrome, a disease arising from germline mutations in DNMT3B. Centromeric and pericentromeric regions are essential for chromosome condensation and the fidelity of segregation. Centromere regions contain distinct epigenetic marks, including dense DNA hypermethylation, yet the mechanisms by which DNA methylation is targeted to these regions remains largely unknown. In the present study, we used a yeast two-hybrid screen and identified a novel interaction between DNMT3B and constitutive centromere protein CENP-C. CENP-C is itself essential for mitosis. We confirm this interaction in ...
Ovarian cancer has a poor prognosis. Most patients are diagnosed with ovarian cancer when the disease has reached an advanced stage and cure rates are generally under 30%. Hence, early diagnosis of ovarian cancer is the best means to control the disease in the long term and abate mortality. So far, cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) are the gold-standard tumor markers for ovarian cancer; however, these two markers can be elevated in a number of conditions unrelated to ovarian cancer, resulting in decreased specifically and positive predictive value. Therefore, it is urgent to identify novel biomarkers with high reliability and sensitivity for ovarian cancer. In this study for the first time, we identified a member of the centromere protein (CENP) family, CENPK, which was specifically upregulated in ovarian cancer tissues and cell lines and the overexpression of which was associated with poor prognoses in patients with ovarian cancer. In addition, the presence of CENPK
The HTR12 protein is a centromere-specific histone H3 variant in A. thaliana, and was shown to colocalize with the 180 bp repetitive sequences of all centromeres (Talbert et al., 2002). The Zea mays centromeric histone H3, CENH3 was also detected at the kinetochore regions of the centromere and colocalized with centromere-specific tandem repeat CentC and with centromeric retroelement CRM (Zhong et al., 2002). These results indicate wide conservation of CENP-A-like proteins and their close relationship to the centromeric satellites. In our study, the spatial relationship between HTR12 protein and 180 bp repetitive sequences was investigated by sequential combination of immunolabeling and FISH. In the cell cultures studied here, drastic changes in the copy numbers of 180 bp repetitive sequences had occurred, however, all chromosomes carried the 180 bp repetitive sequences despite their variation in size (Fig. 1E,F). For chromosomes with low numbers of 180 bp repetitive sequences, ...
Saffery, Richard, Sumer, Huseyin, Hassan, Sara, Wong, Lee H, Craig, Jeffrey M, Todokoro, Kazuo, Anderson, Melissa, Stafford, Angela and Choo, KH Andy 2003, Transcription within a functional human centromere, Molecular cell, vol. 12, no. 2, pp. 509-516, doi: 10.1016/S1097-2765(03)00279-X. ...
In order to define a functional human centromere sequence, an artificial chromosome was constructed as a reproducible DNA molecule. Mammalian telomere repeats and a selectable marker were introduced into yeast artificial chromosomes (YACs) containing alphoid DNA from the centromere region of human c …
GF ID Scm3 #=GF AC PF10384.8 #=GF DE Centromere protein Scm3 #=GF AU Mistry J, Wood V #=GF SE Pfam-B_19394 (release 21.0) #=GF GA 24.30 24.30; #=GF TC 24.60 24.40; #=GF NC 24.20 24.00; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 17548816 #=GF RT Scm3, an essential Saccharomyces cerevisiae centromere protein #=GF RT required for G2/M progression and Cse4 localization. #=GF RA Stoler S, Rogers K, Weitze S, Morey L, Fitzgerald-Hayes M, Baker #=GF RA RE; #=GF RL Proc Natl Acad Sci U S A. 2007;104:10571-10576. #=GF RN [2] #=GF RM 17704645 #=GF RT Domain Architectures of the Scm3p Protein Provide Insights into #=GF RT Centromere Function and Evolution. #=GF RA Aravind L, Iyer LM, Wu C; #=GF RL Cell Cycle. 2007; [Epub ahead of print] #=GF RN [3] #=GF RM 19563746 #=GF RT Common ancestry of the CENP-A chaperones Scm3 and HJURP. #=GF RA Sanchez-Pulido L, Pidoux AL, Ponting CP, Allshire RC; #=GF RL Cell. 2009;137:1173-1174. ...
TY - JOUR. T1 - Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction. AU - Luce, Amy C.. AU - Sharma, Anupma. AU - Mollere, Oliver S.B.. AU - Wolfgruber, Thomas K.. AU - Nagaki, Kiyotaka. AU - Jiang, Jiming. AU - Presting, Gernot G.. AU - Dawe, R. Kelly. PY - 2006/11/6. Y1 - 2006/11/6. N2 - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.. AB - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Track indicating the location of the centromere sequences. Centromeres are specialized chromatin structures that are required for cell division. These genomic regions are normally defined by long tracts of tandem repeats, or satellite DNA, that contain a limited number of sequence differences to distinguish the linear order of repeat copies. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. Unlike all previous versions of the human reference assembly, where the centromere regions have been represented by a multi-megabase gap, GRCh38 incorporates centromere reference models that provide an initial genomic description derived from chromosome-assigned whole genome shotgun (WGS) read libraries of alpha satellite. Each reference model provides an approximation of the true array sequence organization. Although the long-range repeat ordering is not expected to represent the true organization, the submissions are expected to provide a ...
Buy UA62784 (CAS 313367-92-9), a centromere protein E (CENP-E) inhibitor, from Santa Cruz. Purity: ≥98%, Molecular Formula: C23H15NO3, MW: 353.37
Experimental stimulation of TLR9 often utilizes phosphorothioate (PS)-ODN, in which one of the backbone oxygens is replaced by sulfur, rendering these ODN more nuclease-resistant than "natural" ODN with a phosphodiester (PD) backbone. These ODN make effective artificial ligands and can be divided into Class A (also known as D-type), ClassB (also known as K-type), and Class C ODNs; each with distinct immunological effects (5). CpG-A and CpG-C ODNs are strong inducers of type I interferons (6), particularly in plasmacytoid dendritic cells (pDCs),whereas CpG-B ODNs are weak inducers of IFN-αβ (5;7;8), but they induce DCs to mature and produce the tumor necrosis factor (TNF)-α cytokine (5;7) ...
The centromere is the structure at the center of every X-shaped chromosome, where cells attached the long, thin spindles that pull the two copies of DNA apart during cell division. A new technique makes it much easier to study centromeres, and look for links to conditions such as Down syndrome.
Plasmid mRuby2-CENPB-N-22 from Dr. Michael Davidsons lab contains the insert CENPB and is published in Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. This plasmid is available through Addgene.
... : centripetally, centrism, centrist, centrobaric, centroclinal, centrode, centroid, centroidal, centroids, centrolecithal, centromere, centromeric, centrosome, centrosomic, centrosphere, centrosymmetric, centrum, centry, cents, centu...
Chicken (Gallus gallus domesticus, GGA) and Japanese quail (Coturnix coturnix japonica, CCO) karyotypes are very similar. They have identical chromosome number (2n = 78) and show a high degree of synt
Centromeres mediate the conserved and essential process of chromosome segregation, yet centromeric DNA and the centromeric histone, CENP-A, are rapidly evolving. The rapid evolution of loop 1 (L1) of Drosophila CENP-A is thought to modulate the DNA-binding preferences of CENP-A to suppress centromere drive, the preferential transmission of chromosomes with expanded centromeric satellites during female meiosis. Consistent with this model, CENP-A from D. bipectinata (bip) fails to localize to D. melanogaster (mel) centromeres due to amino acid differences between mel and bip L1. Here, I show that this result is, in fact, due to the inability of the mel CENP-A chaperone, CAL1, to incorporate bip CENP-A into chromatin. Co-expression of bip CENP-A and bip CAL1 in mel cells restores centromeric localization, and similar findings apply to other Drosophila species. Furthermore, two co-evolving regions, CENP-A L1 and the CAL1 N-terminus, are identified as critical for lineage-specific CENP-A incorporation.
Hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide and metastasis is regarded as the major cause of HCC-associated lethality. In this study, we have analysed the TCGA whole-transcriptome sequencing data of paired human HCC samples (n=50), and identified Forkhead Box M1 (FOXM1) and Centromere Protein F (CENPF) to be the top-listing upregulated genes. Interestingly, both of them are the essential components in cell-cycle progression. FOXM1 encodes for the cell-cycle-dependent transcription factor that regulates genes for DNA replication and mitosis, while CENPF encodes for the centromere protein that is required for kinetochore function and chromosome segregation in mitosis.. We hypothesized that the upregulation of FOXM1-CENPF signaling axis may drive hepatocarcinogenesis. In our human HCC cohort (n=34), FOXM1 and CENPF were shown to be upregulated compared with the non-tumorous liver tissues, and their mRNA expressions were positively correlated (p,0.0001). ...
Hypopharyngeal squamous cell cancer (HSCC) is a rare malignancy accounting for approximately 0.5% of all human malignancies and representing about 3% to 5% of all head and neck cancers (Hoffman et al. 1998) (Cooper et al., 2009). Most patients with HSSC share a common history of tobacco and/or alcohol abuse. Despite improvements in surgical and chemoradiation approaches, most patients received an initial diagnosis in the advanced cancer stage, which results in high mortality. Over the past decade, the 5-year survival rate has been about 30% (Cooper et al., 2009). Surgery or surgery with radiation is considered valid therapy ( Hoffman et al., 1997; Gourin et al., 2004). Nevertheless, most patients experience swallowing or speech difficulties after curative resection. Even worse, some patients still experience cancer relapse after complete resection of primary tumors. Relapse often cannot be reliably and timely diagnosed for patients with malignancies or HSCC after primary treatment. Therefore,
Centromeres are specialized chromatin structures that are required for cell division. The composition of these regions is complex, as they are made up of a series of tandem repeats that are arranged into nearly identical multi-megabase arrays. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. The Human Genome Project (HGP) employed a clone based strategy (largely BAC clones) to produce the reference assembly, but cloning centromere sequences generally requires special effort, and isnt readily applicable to all human centromeres (see Kouprina et al., 2003 for one such effort). With the recent widespread adoption of whole genome sequencing (WGS), there are clearly alpha-satellite sequences in the reads produced, but assembling these sequences into faithful representations of centromeres using standard techniques is impossible due to the repetitive nature of these sequences. In all previous versions of the human reference assembly, the ...
Centromeres are specialized chromatin structures that are required for cell division. The composition of these regions is complex, as they are made up of a series of tandem repeats that are arranged into nearly identical multi-megabase arrays. The size and repetitive nature of these regions mean they are typically not represented in reference assemblies. The Human Genome Project (HGP) employed a clone based strategy (largely BAC clones) to produce the reference assembly, but cloning centromere sequences generally requires special effort, and isnt readily applicable to all human centromeres (see Kouprina et al., 2003 for one such effort). With the recent widespread adoption of whole genome sequencing (WGS), there are clearly alpha-satellite sequences in the reads produced, but assembling these sequences into faithful representations of centromeres using standard techniques is impossible due to the repetitive nature of these sequences. In all previous versions of the human reference assembly, the ...
Centromere DNA element II (CDEII) of budding yeast centromeres is an AT-rich sequence essential for centromere (CEN) function. Sequence analysis of Saccharomyces cerevisiae CDEIIs revealed that A(5-7)/T(5-7) tracts are statistically overrepresented at the expense of AA/TT and alternating AT. To test the hypothesis that this nonrandom sequence organization is functionally important, a CEN library in which the CDEII sequences were randomized was generated. The library was screened for functional and nonfunctional members following centromere replacement in vivo. Functional CENs contained CDEIIs with the highly biased A(n)/T(n) run distribution of native centromeres, while nonfunctional CDEIIs resembled those picked from the library at random. Run content, defined as the fraction of residues present in runs of four or more nucleotides, of the functional and nonfunctional CDEII populations differed significantly (P | 0.001). Computer searches of the genome for regions with an A + T content comparable to
This entry represents a C-terminal domain found in members of the T complex protein 10 family. This domain contains unusual G repeats [(PUBMED:11003675)]. Centromere protein J, a member of the TCP10 family, inhibits microtubule nucleation from the centrosome and depolymerises taxol-stabilised microtubules [(PUBMED:12068715)]. T-complex is involved in spermatogenesis in mice [(PUBMED:15047868 ...
PcG bodies are associated with kinetochores in three different cell lines. Human centromeres were localized using an autoimmune sera against kinetochores, an
Any antibody to nuclear components is an ANA. Most patients with ANAs do not have SLE, but most people with SLE have ANAs. The most common screening test is IIF on rodent liver or human epithelial (HEp2) tissue,3 although ELISA tests are available.4,5 Lupus erythematous cells simply represent nuclei opsonised by ANAs and are no longer used in diagnosis. Although ANAs are very sensitive for SLE, positive ANAs are common, especially in unwell elderly individuals.6-8 Therefore, ANAs have low PPV for SLE in unselected populations or when present in low titres,6,9 and are not diagnostic. One in three healthy people have detectable ANAs on HEp-2 cells at a screening dilution of 1/40 and one in 20 will be positive at 1/160. HEp-2 cells produce more positive ANAs than rat tissue, and some ANAs (for example, anticentromere antibodies) can only be reliably detected on HEp-2 substrate. Although "ANA negative" SLE is reported,10 it is not clear whether this is the result of a technical artifact or whether a ...
Chromosomes can be classified based on the following except ____. a) centromere location b) centromere size c) number of centromeres d) centromere duration
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Caroli disease is a type of inherited condition that affects the biliary tract. The main signs of Caroli disease are generally...
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中節,人體部位名。手足指(趾)的第二節。手足大指(趾)無此節。. 染色體著絲粒(centromere),又稱中節,主要作用是使複製的染色體在有絲分裂和減數分裂中可均等地分配到子細胞中。在很多高等真核生物中,著絲粒看起來像是在染色體一個點上的濃縮區域,這個區域包含著絲點(希臘語kínesis 運動;chóros 部位),又稱主縊痕(primary constriction)。. 著絲粒(染色體的主縊痕)為染色質的結構,將染色體分成二臂,在細胞分裂前期和中期,把兩個姐妹染色單體連在一起,到後期兩個染色單體的著絲粒分開。著絲粒兩側各有一個由蛋白質構成的3層盤狀特化結構,為非染色體性質物質的附加物,稱為著絲點。. 在大部分真核生物中每個紡錘絲附著在不同的著絲粒上。如啤酒酵母(Saccharomyces ...
The accurate distribution of genetic information to daughter cells during cell division relies on the physical attachment of chromosomes to spindle microtubules mediated by kinetochores. Kinetochores are large protein assemblies deposited at specific chromosomal loci known as centromeres [1], [2], [3]. Defective centromere function results in chromosome segregation errors that can contribute to genomic instability implicated in cancer [4]. Hence, understanding the molecular mechanisms that promote kinetochore establishment and maintenance at centromeres is of prime importance.. The location of most eukaryotic centromeres is determined by the assembly of specialized chromatin composed of nucleosomes in which canonical histone H3 is replaced by the centromere‐specific H3 variant CENP‐A in vertebrates and Cnp1 (CENP‐ACnp1) in Schizosaccharomyces pombe [3], [5]. Thus, the establishment and maintenance of kinetochores requires CENP‐A to be recruited to and deposited at centromeres. In S. ...
Centromeres are the specialized chromosomal sites necessary for poleward movement during mitosis and meiosis in eukaryotes. Commonly, a centromere is evident as a prominent constriction within the heterochromatin of each metaphase chromosome. The attachment to and movement of chromosomes along the spindle is mediated by the proteinaceous kinetochores, which form at the centromeres during cell division.. Despite this highly conserved function, centromeric DNA sequences are not conserved between organisms. For example, human centromeres consist of large blocks (200 kb to several megabases) of tandemly repeated 171-bp α-satellite (Willard, 1998), but the sequences can differ from those of apes on homologous chromosomes (Haaf and Willard, 1997). Similarly, Drosophila melanogaster centromeric regions contain blocks of 5- to 12-bp satellite repeats that do not appear to be shared by homologous centromeres of sibling species (Lohe and Brutlag, 1987).. Plant centromeric regions resemble their mammalian ...
The localization of yeast centromeres and the 2-μm circle. Centromeres cluster close to the spindle pole body in late G1, but do not localize to the extreme nu
The tissue associations are derived from manually curated knowledge in UniProtKB and via automatic text mining of the biomedical literature, which has not been manually verified. The confidence of each association is signified by stars, where ★★★★★ is the highest confidence and ★☆☆☆☆ is the lowest. Download files from earlier versions are archived on figshare.. Each tissue-gene association is based on a text-mining score, which is proportional to 1) the absolute number of comentionings and 2) the ratio of observed to expected comentionings (i.e. the enrichment). These scores are normalized to z-scores by comparing them to a random background. This is represented by stars, each star corresponding to two standard deviations above the mean of the background distribution.. Developed by Alberto Santos, Oana Palasca, Christian Stolte, Kalliopi Tsafou, Sune Frankild, Janos Binder, Sean ODonoghue, Jan Gorodkin, and Lars Juhl Jensen from the Novo Nordisk Foundation Center for Protein ...
Neocentromere activation requires centromere juxtaposition: Here we describe irradiation-mutagenesis experiments designed to identify the mechanism of neocentromere formation in D. melanogaster. Prior to this study, two models existed to explain the generation of neocentromeres in Drosophila and Homo sapiens-derepression of latent centromere-competent euchromatic sequences vs. centromere spreading (Choo 1997a, 1998). We distinguished between these models through a genetic assay for neocentromere activation and recovery. Three substrate chromosomes were irradiated, and an identical 290-kb test segment was liberated and genetically assayed for neocentromere activity. The three test segments were identical in molecular structure and differed only in their chromosomal context. In Dpγ238, the test segment was juxtaposed to an active centromere; in Dp8-23, the test segment was juxtaposed to centric, but centromerically inert DNA; in Tγ1337, the test segment was juxtaposed to euchromatin. ...
The organization, evolution and function of eukaryotic centromeres represent a deficiency in our understanding of genome biology. The discovery of human clinical neocentromeres and ENCs has further complicated, on one hand, our understanding of the centromere. On the other hand, neocentromeres and ENCs have allowed an initial dissection of centromere complexity. They have made evident, for instance, its epigenetic nature. The ENC analysis we have accomplished in the present study has contributed to the identification of factors that, very likely, play a crucial role in ENC progression and fixation in the population. We have provided strong evidence that the pericentromeric duplication activity is an intrinsic property of ENCs. This conclusion was mainly supported by FISH experiments using species-specific BAC clones that detected SDs around the centromere in almost all studied ENCs. A deep restructuring was particularly evident in MMU17 (human 13) and MMU2 (human 3). The latter ENC showed a ...
Cnp1, Mis6, and Mis13 are required for localizing condensin at the kinetochore. (A) Cells cultured at 26°C in EMM2 were observed for the colocalization of Cnd1-GFP with Mis12-RFP, a centromere/kinetochore protein. The numbers in the right panels indicate time in minutes. The enlarged images of Cnd1-GFP (left), Mis12-RFP (middle), and the merged images (right) at 13 min are shown in the insets. (B) Cut14-GFP and Sad1-RFP were observed in the wild-type, mis6-302, cnp1-1, mis13-1, mis16-53, and mis18-262 mutants cultured at 26°C and were shifted to 36°C for 8 h. (C) Chromosomally integrated Cnp1/CENP-A-GFP expressed under the native promoter was observed in the wild-type and cut14-208 mutant cultured at 36°C for 2 h. (D) A ChIP assay was performed using extracts of block-released nda3-311 mutant that expressed Cut14-Flag. The probes were from the central centromere, cnt1 (c10, c9, and c7.5), imr1 (c4 and c1), the outer centromere dg, and the noncentromeric lys1+. WCE, whole cell extract; IP, ...
Our lab is interested in the epigenetic inheritance and organization of centromeres. The DNA sequence independent transmission of centromere identity through many cell generations is highly relevant for proper genome regulation and when perturbed can lead to genome instability and cellular malfunction. We use the fruit fly Drosophila melanogaster and human cells as a model organism to address the following questions: How is the epigenetic identity of centromeres regulated?. Centromeres are found at the primary constriction of chromosomes in mitosis where they remain connected before cell division. This structure is essential for an equivalent chromosomes distribution to the daughter cells. The centromere specific histone H3-variant CENP-AcenH3 is essential for kinetochore formation and centromere function. We have previously established a biosynthetic approach to target dCENP-AcenH3 to specific non-centromeric sequences such as the Lac Operator and follow the formation of functional ...
You can find three classes of RNA polymerase enzyme (RNAPs I, II and III). elevated occurrence of renal participation (29.0%, cf. remainder, 11.3%; < 0.05; RR 2.6), with 40% of anti-RNAP I/II/III sufferers having renal disease. In the meantime, the current presence of anti-centromere antibodies (ACA) was connected with limited cutaneous SSc (lc-SSc) (100.0%; cf. […] ...
Centromeres arent circles like theyre drawn in the books, theyre just repetitie DNA sequences, and they look just like any other part of the chromosome... like DNA. And im guessing they get separated when the centrioles pull each chromosomes chromatid away in opposite directions through the nuclear spindles (which are like strings that hook to each chromatid ...
Meštrović, Nevenka; Pavlek, Martina; Car, Ana; Castagnone-Sereno, Philippe; Abad, Pierre; Plohl, Miroslav (2013) Conserved DNA motifs, including the CENP-B box-like, are involved in satellite DNA array rearrangements. PLoS ONE, 8 (6). e67328/1-e67328/10. ISSN 1932-6203 ...
494; 1536; L895; S425; S424; 1048; T048; L030; 103; S175; S468; S832; S831; Q888; M365; CA19; 684; CA27; 698; Q620; 6050; M301; PCAN; X621; RECT; 130; 1755; 383; X587; 977; 1460; 0616; 0618; VAP1; ACT4; S602; C17; C12; C15; X860; T281; 1565; L067; L379; 2560; L529; T080; Q371; 433; 1717; 20; 21; NK57; CD57; 1065; 1532; CENP; 392; SMAC; CM24; 6520; 6513; 6510; S006; T261; T039; Q258; 135; S488; A870; 109; C1ES; X599; A125; Q469; S503; 468; V111; 4962; T449; M162; CDIF; 4964; T046; A867; S994; COC; 485; X605; X601; L251; 478; 3246; S240; 551; 0464; 0459; 666; 1978; Q240; 12; 2676; A937; T112; S513; A072; U204; S239; X328; S728; S726; T358; A322; A462; T228; 572; S867; M273; 110; CPKI; 712; S252; S120; 112; 497A; 2007; CRYO; Y224; A006; M371; N394; URCS; BFCG; 6011; EYCU; FGCO; FGCL; GENC; S491; Q602; NASC; SINC; S150; 7457; THAB; WOUC; L776; YEAS; YSTC; 2003; 7962; BLCU; EACU; STCU; A604; URCU; A900; S736; 1074; 543; 5091; 5092; 5705; X624; CF60; X622; X613; Q190; X708; 619; 546; A052; CYTI; NGYN; S410;
Kelly Dawe. Distinguished Research Professor What are plant centromeres made of? How are they inherited, what proteins interact with them, and how do they evolve? What are centromeres? For over twelve years our lab has been working through the answers to these questions.lab was founded with the goal of understanding plant kinetochores. We have made good progress mostly by making specific antisera and combining the power of maize cytogenetics with 3D light microscopy. Much of our effort has focused on the inner kinetochore proteins Centromeric Histone H3 (CENH3) and Centromere Protein C (CENP-C), as well as MAD2, a spindle checkpoint protein that localizes to the outer kinetochore. We have worked on a serine-50 phosphorylated form of CENH3, NDC80, and several other kinetochore proteins. Our long-term goal is to identify the complete collection of inner kinetochore proteins, and to develop a model for how these proteins are organized. We intend to pursue the tried-and-true method of identifying ...
This organism was chosen because it has epigenetically defined "regional centromeres" whose chromatin and protein compositions are similar to those of their human counterparts, to identify factors responsible for the replacement of histone H3 with CENP-A at centromeres.. In this report, the KAIST research group systematically analyzed the roles of the ATP-dependent chromatin-remodelers in the centromeric chromatin assembly of fission yeast as they serve as strong candidates for such factors ...
We study the spectrum and mechanisms of telomere function. Recent highlights include the discovery of a mode by which telomerase-negative cells can use generic heterochromatin to protect ends. We have also expanded the telomeric repertoire, finding that telomeres control meiotic spindle formation and centromere assembly; these principles apply to proliferating cells as well, as centromeres control the decision to mount mitotic spindle assembly.. Keywords: Telomeres / Centromeres / DNA damage response / fission yeast / meiosis / chromatin & nuclear organization. Subject area(s): Cell Cycle , Chromatin & Transcription , Genome Stability & Dynamics. ...
A 59-year-old man with limited cutaneous systemic sclerosis (lcSSc), who had retired after many years as a construction worker, presented with bilateral knee deformity without discomfort or pain. A diagnosis of lcSSc had been made 13 years previously on the basis of sclerodactyly, digital ulceration, calcinosis, and positive anticentromere antibodies. Treatment by ilomedine was initiated in 1999 … ...
Maize (is activated by Fru-6-P (F-6-P) and inhibited by inorganic phosphate (Pi) whereas the AGPase is activated by Fru-1 6 but inhibited by AMP. (small subunit homotetramer; Jin et al. 2005 HA14-1 Although both buildings reveal inactive conformations because of high concentrations of ammonium sulfate in the crystallization buffer important info about potential substrate-binding sites was forecasted by molecular modeling predicated on the known buildings of thymidilyltransferases. While this course of enzymes most likely binds glucose phosphates very much the same as AGPases thymidilyltransferases arent governed allosterically. Both HA14-1 AGPase crystal buildings claim that the enzyme features being a dimer of dimers like the system suggested for the enzyme based on ligand-binding research (Haugen and Preiss 1979 All obtainable evidence network marketing leads to the final outcome that tetramers are necessary for AGPase catalytic activity. Both obtainable AGPase crystal buildings present two ...
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Read "Recognition of A. thaliana centromeres by heterologous CENH3 requires high similarity to the endogenous protein, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Article source: http://theworldseeds.cn/index.php?p=152804 (Translated by Google Translate) The centromere and its surroundings are the fastest-evolving and…
Core particle is an octamer of 4 histone proteins; H2A, H2B, H3 & H4 each present twice. DNA strand of 146 bp surrounds the octamer. It makes 1¾ coil around the octamer ...
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On Wed 2017-01-04 18:59:54 -0500, alex at centromere.net wrote: , Trevor and I are at RWC2017. Is anyone on this list also attending? Im also at RWC2017; i dunno the full list of who all is subscribed here. Nice to see Trevor get recognized with the Levchin award today, though :) --dkg -------------- next part -------------- A non-text attachment was scrubbed... Name: signature.asc Type: application/pgp-signature Size: 832 bytes Desc: not available URL: ,http://moderncrypto.org/mail-archive/noise/attachments/20170105/0e32f6a4/attachment.sig ...
Our research program is focused on the important basic question of how chromosomes are segregated during cell division to ensure the complete and accurate inheritance of the genome. Chromosome instability is a hallmark of cancer and can drive tumorigenesis. Therefore, how centromere specification is controlled is a basic biological question with great therapeutic potential. Centromeres are specified by the incorporation of a histone variant CENP-A, and stable inheritance of this locus is control...[Read full text]Our research program is focused on the important basic question of how chromosomes are segregated during cell division to ensure the complete and accurate inheritance of the genome. Chromosome instability is a hallmark of cancer and can drive tumorigenesis. Therefore, how centromere specification is controlled is a basic biological question with great therapeutic potential. Centromeres are specified by the incorporation of a histone variant CENP-A, and stable inheritance of this locus ...
Model for heterochromatin assembly and spreading at S. pombecentromeric outer repeats. Heterochromatic centromere sequences (yellow arrow) are transcribed by RNA Polymerase II. These centromere transcripts are targeted by RITS via siRNA loaded Ago1. Association of RITS with centromere heterochromatin is strengthened by binding of Chp1 to H3mK9. RITS activity can recruit both CLRC, via interactions with Stc1, and RDRC resulting in spreading of H3mK9 and amplification of siRNAs, respectively (see text for details). dsRNA generated either by bi-directional transcription from centromere promoters (black arrows) or by RDRC activity is recognized and processed by Dicer (Dcr1). The resulting centromere siRNAs are then loaded onto Ago1 first in the ARC complex and then in RITS ...
Wow, I count 14 question marks in one post. Is that a record?. Ill try to hit as many of the question marks as possible.. 1. So wouldnt the first human (with 46) have significant trouble reproducing? Not necessarily. Research has been performed to investigate just this question and found that fertility is effected only minimally. Essentially, it turns out that some centromeres are better at attracting the kinetochore machinery than others and thus outcompete the neighboring centromere for resources [a]. Thus, even in the case of a fusion, only one centromere will remain active.. 2. Could he or she reproduce with an ape mate? No. Nor would they likely be interested in doing so - any more than you are interested in mating with a gorilla. When a chromosomal fusion occurs in one individual human, they would look no different than any other human. All the same genes are still there, being expressed the same way. When the fusion of chromosomes 12 and 13 occurred in the human lineage, nobody would ...
Wow, I count 14 question marks in one post. Is that a record?. Ill try to hit as many of the question marks as possible.. 1. So wouldnt the first human (with 46) have significant trouble reproducing? Not necessarily. Research has been performed to investigate just this question and found that fertility is effected only minimally. Essentially, it turns out that some centromeres are better at attracting the kinetochore machinery than others and thus outcompete the neighboring centromere for resources [a]. Thus, even in the case of a fusion, only one centromere will remain active.. 2. Could he or she reproduce with an ape mate? No. Nor would they likely be interested in doing so - any more than you are interested in mating with a gorilla. When a chromosomal fusion occurs in one individual human, they would look no different than any other human. All the same genes are still there, being expressed the same way. When the fusion of chromosomes 12 and 13 occurred in the human lineage, nobody would ...
Hi, my name is Kristi. I was just diagnosis with CREST. My sister has LUPUS (involving kidneys) and after participating in a LUPUS genetic study for her, they found a high positive ANA in me. I went to my general practitioner and they did another ANA and it was negative. I went to a Rheumatologist and she told me they have to specifically ask for the anticentromere pattern of ANA or they will not test for this rare pattern and that is why I got the negative result the second time. The rheumatologist repeated the ANA once more on me, specifically asking for the centromere pattern and it came back with a high positive of 1:1,280. She diagnosed me with CREST due to my raynauds and daily severe heart burn. I also have the beginning skin changes on my fingers. She ordered the lung PFT, Echocardiogram heart test and a Barrium swallow to check the condition of my esophagus. She thinks it is limited, not diffuse, so I guess I am lucky there. Any advice or encouragement is welcome. Thanks, Kristi ...
(2015) Barth et al. Data in Brief. Centromeres of higher eukaryotes are epigenetically defined by the centromere specific histone H3 variant CENP-ACID. CENP-ACID builds the foundation for the assembly of a large network of proteins. In contrast to mammalian systems, the protein composition of Dro...
To determine the clinical significance of anticentromere (ACA) and anti Scl-70 antibodies in an English population with connective tissue diseases, we examined the sera of 150 patients, including 40...
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation ...
Analysis of meiotic tetrads is routinely used to determine genetic linkage in various fungi. Here we apply tetrad analysis to the study of genetic linkage in a vertebrate. The half-tetrad genotypes of gynogenetic diploid zebrafish produced by early-pressure (EP) treatment were used to investigate the linkage relationships of two recessive pigment pattern mutations, leopard (leo) and rose (ros). The results showed that ros is tightly linked to its centromere and leo maps 31 cM from its centromere. Analysis of half-tetrads segregating for ros and leo in repulsion revealed no homozygous ros individuals among 32 homozygous leo half-tetrads--i.e., a parental ditype (PD) to nonparental ditype (NPD) ratio of 32:0. This result shows that ros is linked to leo, a mutation previously mapped to Linkage Group I. Investigation of PCR-based DNA polymorphisms on Linkage Group I confirmed the location of ros near the centromere of this linkage group. We propose an efficient, generally useful method to assign new ...
The centromere is a special region of a chromosome, usually near the middle. It is where the two identical sister chromatids stay in contact as the chromosome attaches to the spindle in mitosis. The region contains specific types of DNA, which are tandem repetitive sequences (satellite DNA). These sequences bind specific proteins called "cen"-proteins. During mitosis the centromeres can be seen during the metaphase stage as a constriction at the chromosome. At this centromeric constriction the two halves of the chromosome, the sister chromatids, are held together until late metaphase. ...
Results The mHAMIS and the mRSS hand changed synchronously during the first 5 years after disease onset (rs = 0.44, p = 0.001). In the group with high mHAMIS at baseline, both mHAMIS and mRSS hand improved significantly at the first followup (p , 0.05), and the improvement sustained during the followup in the mRSS hand. Patients with antitopoisomerase I and anti-RNA polymerase III antibodies had significantly higher mHAMIS at baseline (p = 0.003) and at the second followup (p = 0.030) compared to patients with anticentromere antibodies. Patients with digital vascular lesions at baseline had significantly higher mHAMIS during the followup (p , 0.05) compared to patients without. The mHAMIS improved significantly during the followup in patients with immunosuppressive treatment in early disease (p , 0.05), but not in patients without this treatment. ...
Alignment of the centromere regions of all sixteen chromosomes. The regions include the Centromere DNA Elements I II and III (CDEI, CDEII and CDEIII). The conserved bases in all centromeres are marked in magenta. The regions with less conserved residues of CDEI and CDEIII are marked in green. The CDEII region which contains more than 90% AT residues has been left white. The multiple sequence alignment was created with PILEUP ...
A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That is, instead of 46 chromosomes, the cell could have 47 with the 47th being very small, roughly 6-10 megabases (Mb) in size instead of 50-250 Mb for natural chromosomes, and able to carry new genes introduced by human researchers. Ideally, researchers could integrate different genes that perform a variety of functions, including disease defense. Alternative methods of creating transgenes, such as utilizing yeast artificial chromosomes and bacterial artificial chromosomes, lead to unpredictable problems. The genetic material introduced by these vectors not only leads to different expression levels, but the inserts also disrupt the original genome. HACs differ in this regard, as they are entirely separate chromosomes. This separation from existing genetic material assumes that no insertional mutants would arise. This stability and accuracy makes HACs preferable to other ...
... centromere protein A), CENPB, CENPC1, and CENPD. The term 'passenger proteins' encompasses a broad collection of proteins that ... Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[5][6][7] ... constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[8] The constitutive proteins ... "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".. *^ Choo, K. H. Andy (1997). The centromere. Oxford [ ...
Centromere protein C 1 is a protein that in humans is encoded by the CENPC1 gene. Centromere protein C 1 is a centromere ... 2002). "Mutational analysis of the central centromere targeting domain of human centromere protein C, (CENP-C)". Exp. Cell Res ... 2004). "In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: ... "Interphase-specific association of intrinsic centromere protein CENP-C with HDaxx, a death domain-binding protein implicated in ...
Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene. This gene encodes a ... The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... "Conservation of the centromere/kinetochore protein ZW10". J Cell Biol. 138 (6): 1289-301. doi:10.1083/jcb.138.6.1289. PMC ...
Centromere protein O is a protein that in humans is encoded by the CENPO gene. CENPO is involved in cell proliferation and cell ... "Entrez Gene: CENPO centromere protein O". Hewitt, Chelsee A.; Ling, King-Hwa; Merson, Tobias D.; Simpson, Ken M.; Ritchie, ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...
Centromere protein F is a protein that in humans is encoded by the CENPF gene. This gene encodes a protein that associates with ... "Entrez Gene: CENPF centromere protein F, 350/400ka (mitosin)". Human CENPF genome location and CENPF gene details page in the ... The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the ... "The cloning and analysis of LEK1 identifies variations in the LEK/centromere protein F/mitosin gene family". J. Biol. Chem. 274 ...
The second is a DNA-binding adaptor protein known as ParR. The last component is a centromere-like region called ParC. The ... The three components, a parC DNA site, and two proteins parR and parM all combine to create the ParMRC system, a type II ... In the cell the ParM protein filaments search for plasmids. Next, they find the ParR and ParC that are headed to DNA molecules ... This type of process using filament forming actin-like protein (ParM) to move DNA to opposite sides of the cell has been ...
Centromere protein H is a protein that in humans is encoded by the CENPH gene. Centromere and kinetochore proteins play a ... "Entrez Gene: CENPH centromere protein H". Fukagawa, T.; Mikami, Y.; Nishihashi, A.; Regnier, V.; Haraguchi, T.; Hiraoka, Y.; ... 2005). "Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy". ... 2007). "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival ...
"Distinct protein interaction domains and protein spreading in a complex centromere". Genes Dev. 14: 783. 2000. PMID 10766735. " ... "A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres". Mol. Cell ... "The chromodomain protein Swi6: a key component at fission yeast centromeres". Science. 269: 1429. 1995. PMID 7660126. " ... "The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin". EMBO J. 26: 4670. 2007. PMID ...
The resulting BLM protein is defective. the defect in RecQ an helicase facilitates the defective unwinding of DNA during ... The recombination needs to occur between the centromere the adjacent gene. This gives an appearance of yellow patches on the ... FRT sites have been inserted transgenically near the centromere of each chromosome arm of Drosophila melanogaster. The FLP gene ... commonly the green fluorescent protein or GFP) and an allele of a gene to be studied (both on chromosomes bearing FRT sites). ...
Centromere protein M also known as proliferation associated nuclear element 1 (PANE1) is a protein that in humans is encoded by ... Centromere protein M us involved in centromere assembly and immune response. GRCh38: Ensembl release 89: ENSG00000100162 - ... "Entrez Gene: CENPM centromere protein M". Brickner AG, Evans AM, Mito JK, Xuereb SM, Feng X, Nishida T, Fairfull L, Ferrell RE ... Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (February 2004). "Proteomics analysis of the centromere complex from HeLa ...
This alternative form is composed of core RFC proteins RFC2, RFC3, RFC4, and RFC5, but replaces the RFC1 protein with cohesion ... High levels of cohesin binding are observed at the centromere. Cohesin is also loaded at cohesin attachment regions (CARs) ... SMC1ß, REC8 and STAG3 are meiosis specific cohesin proteins. The STAG3 protein is essential for female meiosis and fertility. A ... The cohesin ring is composed of two SMC (structural maintenance of chromosomes) proteins and two additional Scc proteins. ...
Centromere protein A, also known as CENPA, is a protein which in humans is encoded by the CENPA gene. Centromeres are the ... "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated". ... Uren AG, Wong L, Pakusch M, Fowler KJ, Burrows FJ, Vaux DL, Choo KH (2001). "Survivin and the inner centromere protein INCENP ... The CENPA gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for ...
All centromeres are associated with centromere protein A (CENPA). CENPA also been widely studied as an important player in ... Centromeres are well-associated with specific proteins that are involved in the formation of the kinetochore and the mitotic ... Overexpression of CENPA and CENPH (centromere protein H) is also associated with colorectal cancer. It may be worth noticing ... centromere regulation as it binds and specifies centromere location for both normal centromeres and neocentromeres, regardless ...
Diseases with ATA are autoimmune disease because they react with self-proteins. They are also referred to as anti-DNA ... However, CREST syndrome is more closely associated with Anti-centromere antibodies. Scl-70 antibodies are associated with more ... In addition to HLA-DR, the protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (1p13.2 - PTPN22), "CT/TT" genotype ... 2006). "Association of the protein tyrosine phosphatase, non-receptor type 8 R620W polymorphism with anti-topoisomerase I- and ...
Jiang W, Koltin Y (1996). "Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae ... For example, sumoylation may affect a protein's localization in the cell, its ability to interact with other proteins or DNA. ... Four alternatively spliced transcript variants encoding the same protein have been found for this gene. The UBC9 protein ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134 ...
3-9 encode centromere-associated proteins which complex with the heterochromatin component M31". The EMBO Journal. 18 (7): 1923 ... Chromobox protein homolog 1 is a protein that in humans is encoded by the CBX1 gene. The protein is localized at ... 3-9 encode centromere-associated proteins which complex with the heterochromatin component M31". The EMBO Journal. 18 (7): 1923 ... "Heterochromatin protein HP1Hsbeta (p25beta) and its localization with centromeres in mitosis". Chromosoma. 106 (1): 11-9. doi: ...
These copies are referred to as sister chromatids and are attached by DNA element called the centromere. The main events of ... powered by centrosome associated motor proteins. Interdigitated interpolar microtubules from each centrosome interact with each ... Condensed chromosomes consist of two sister chromatids joined at the centromere. During prophase in animal cells, centrosomes ... by each centromere. Interpolar microtubules from both centrosomes interact, joining the sets of microtubules and forming the ...
Centromere protein R is a protein that in humans is encoded by the ITGB3BP gene. ITGB3BP has been shown to interact with: CD61 ... Talukder AH, Gururaj A, Mishra SK, Vadlamudi RK, Kumar R (2004). "Metastasis-associated protein 1 interacts with NRIF3, an ... "Entrez Gene: ITGB3BP integrin beta 3 binding protein (beta3-endonexin)". Fujimoto TT, Katsutani S, Shimomura T, Fujimura K ( ... PKC theta-dependent protein". J. Cell Biol. 147 (5): 1073-84. doi:10.1083/jcb.147.5.1073. PMC 2169340 . PMID 10579726. Ohtoshi ...
Centromere protein K is a protein that in humans is encoded by the CENPK gene. CENPK has been shown to interact with SOX6. ... "Entrez Gene: CENPK centromere protein K". Yamashita A, Ito M, Takamatsu N, Shiba T (Sep 2000). "Characterization of Solt, a ... Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (Feb 2004). "Proteomics analysis of the centromere complex from HeLa ... Yamashita A, Ito M, Takamatsu N, Shiba T (Sep 2000). "Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in ...
Centromere protein J is a protein that in humans is encoded by the CENPJ gene. It is also known as centrosomal P4.1-associated ... "Entrez Gene: CENPJ centromere protein J". Al-Dosari MS, Shaheen R, Colak D, Alkuraya FS (Jun 2010). "Novel CENPJ mutation ... "Protein 4.1 R-135 interacts with a novel centrosomal protein (CPAP) which is associated with the gamma-tubulin complex". ... "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): ...
Centromere protein I is a protein that in humans is encoded by the CENPI gene. The product of this gene is involved in the ... "Entrez Gene: CENPI centromere protein I". Human CENPI genome location and CENPI gene details page in the UCSC Genome Browser. ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human ...
Centromere protein T is a protein that in humans is encoded by the CENPT gene. GRCh38: Ensembl release 89: ENSG00000102901 - ... "Entrez Gene: CENPT centromere protein T". Human CENPT genome location and CENPT gene details page in the UCSC Genome Browser. ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human ...
Centromere protein N is a protein that in humans is encoded by the CENPN gene. GRCh38: Ensembl release 89: ENSG00000166451 - ... "Entrez Gene: CENPN centromere protein N". Human CENPN genome location and CENPN gene details page in the UCSC Genome Browser. ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2006). "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres". Nat. ...
Centromere protein U is a protein that in humans is encoded by the MLF1IP gene. GRCh38: Ensembl release 89: ENSG00000151725 - ... 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a ... 2005). "The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage". Mol. Cell. Biol. 25 (23): ... 2007). "MLF1-interacting protein is mainly localized in nucleolus through N-terminal bipartite nuclear localization signal". ...
Within the primary constriction there is a clear zone called Centromere. The centromere with the DNA and histone proteins bound ... Each chromosome has one centromere, with one or two arms projecting from the centromere, although, under most circumstances, ... Most eukaryotic chromosomes include packaging proteins which, aided by chaperone proteins, bind to and condense the DNA ... if the centromere is located in the middle of the chromosome or a two-arm structure if the centromere is located near one of ...
2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell ... "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell Biol. 6 ... "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell Biol. ... 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes Dev. ...
UNKL: encoding protein RING finger protein unkempt-like. *VAT1L: encoding protein Vesicle amine transport protein 1 homolog (T ... acen Centromere. var: Variable region; stalk: Stalk. ... ITFG3: encoding protein Protein ITFG3. *KDM8: encoding protein ... LINC00273 encoding protein Long intergenic non-protein coding RNA 273. *LOC124220: encoding protein Zymogen granule protein 16 ... SHCBP1: encoding protein SHC SH2 domain-binding protein 1. *SLZ1: encoding protein SLX1 structure-specific endonuclease subunit ...
"A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell ... "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell ... The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late ... ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. Zwint-1 is clearly involved in kinetochore ...
A Synaptonemal Complex Protein Promotes Homology-Independent Centromere Coupling Message Subject. (Your Name) has forwarded a ... Regions of synaptonemal complex assembled early in meiosis are often centromere-associated. We propose that centromere coupling ... During meiosis in wild-type diploids, centromere couples are initially nonhomologous and then undergo switching until all ... This transition to homologous coupling depends on Spo11, a protein required for the initiation of meiotic recombination. ...
Evolution of Centromere/Kinetochore Proteins. All centromeres must perform the similar functions of connecting chromosomes to ... that the MIF2 gene of Saccharomyces cerevisiaeencodes a centromere protein with homology to the mammalian centromere protein ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ... Conservation of the Centromere/Kinetochore Protein ZW10. Daniel A. Starr, Byron C. Williams, Zexiao Li, Bijan Etemad-Moghadam, ...
Centromere Protein H and Ki67 in Relapse-free Survival with Hypopharyngeal Cancers. *. Jun-Xi Wang, Ying-Yao Zhang, Xue-Min Yu ... Prognostic relevance of Centromere protein H expression in esophageal carcinoma. *Xian-Zhi Guo, Ge Zhang, +7 authors Mu-Sheng ... Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy.. *Takeshi ... Centromere protein H is a novel prognostic marker for human nonsmall cell lung cancer progression and overall patient survival. ...
Krasikova A, Barbero JL, Gaginskaya E (2005) Cohesion proteins are present in centromere protein bodies associated with avian ... Recent discovery of Centromere protein A (CENP-A)-associated sequences in chicken (Shang et al. 2010) provides an additional ... "mature centromeres." On the other hand, chicken centromeres might represent ancestral centromeres whereas ENC formation ... 2010). One can argue that immunostaining for cohesin-complex proteins that we use to determine centromere position on LBCs is ...
The histone protein CenH3 is both necessary and sufficient to trigger the formation of centromeres and pass them on from 1 ... Centromeres provide a platform for the development of a protein complex known as the kinetochore. During cell division, the ... The step from a DNA-identified centromere in bakers yeast, in which the position "is set in stone", to a protein-defined ... Otherwise, the available CenH3 proteins would be reduced by half after each cell division. In this way, the centromere position ...
The human centromere protein B (CENP-B), one of the centromere components, specifically binds a 17 bp sequence (the CENP-B box ... Centromere protein References *↑ Tanaka Y, Nureki O, Kurumizaka H, Fukai S, Kawaguchi S, Ikuta M, Iwahara J, Okazaki T, ... May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and ... Dna binding protein-dna complex , Helix-turn-helix , Protein-dna complex , Rsgi ...
... the centromere of a higher eukaryote, particularly a plant, the telomere itself, the centromere ... Authentic CEN binding proteins are thus localized at the centromere. The most preferred methods for isolating the genome clones ... Kinetochores are complex nucleo-protein structures, located at the centromeres, responsible for the proper partitioning of the ... Attempts to demonstrate that the S. pombe centromere-specific repetitive elements can function individually as centromeres have ...
Also at the protein level few centromere proteins are conserved in all of these four organisms and many are unique to the ... Thus, despite the differences in the DNA sequences and the proteins that define a centromere, there is an overall structural ... Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite ... Centromere domain organization and histone modifications. Bjerling, Pernilla Södertörn University, Avdelning Naturvetenskap. ...
Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and ... Synaptonemal complex protein 1 - Also known as SYCP1_RAT, Sycp1, Scp1. Major component of the transverse filaments of ... The nascent synapsis generated by SYCP1 is stabilized through interaction with central element proteins SYCE1 and SYCE2 (By ... Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and ...
Since cellular tropism of MV is determined by binding of the H protein to cell surface protein, CD46 or SLAM [20-22], in order ... Ikeno M, Masumoto H, Okazaki T. Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range alpha- ... The chimeric H protein was produced by fusing anti-transferrin receptor (TfR) scFv to the C-terminus of the H protein. ... Successfully, co-transfection of plasmids encoding the chimeric H protein and the F protein into CHO cells, which harbor the ...
Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centromere-associated protein E is a ... Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of ... "Entrez Gene: CENPE centromere protein E, 312kDa". Yen TJ, Li G, Schaar BT, et al. (1992). "CENP-E is a putative kinetochore ... 1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5 ...
In humans, centromere protein B is encoded by the CENPB gene. Centromere protein B is a highly conserved protein that ... Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. ... Centromere protein B is a potential biomarker of small-cell lung cancer. Centromere GRCh38: Ensembl release 89: ENSG00000125817 ... This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and ...
Most plant, animal and fungal centromeres also bind a large protein, centromere protein C (CENP-C), that is characterized by a ... Adaptive evolution of centromere proteins in plants and animals.. Talbert PB1, Bryson TD, Henikoff S. ... Portions of the human CENP-C protein implicated in centromere-targeting (purple bars) and DNA-binding (black bars) are shown at ... The rat CENP-A protein. (a) Alignment of predicted CENP-A proteins of mammals. Relative to other mammalian CENP-As, rat CENP-A ...
Centromere-associated protein-E (CENP-E; kinesin-7) is a kinetochore-associated kinesin motor protein with an essential and ... Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Kenneth W. Wood, Latesh Lad, Lusong Luo, ... Antitumor activity of an allosteric inhibitor of centromere-associated protein-E. Kenneth W. Wood, Latesh Lad, Lusong Luo, ... 2008) Centromere-associated protein E: A motor that puts the brakes on the mitotic checkpoint. Clin Cancer Res 14:7588-7592. ...
Compare centromere protein P ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, ... centromere protein P ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Your search returned 24 centromere protein P ELISA ELISA Kit across 3 suppliers. ...
... a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome ... May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (By ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10116. ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10116. ...
J:57771 Sugata N, et al., Characterization of a novel kinetochore protein, CENP-H. J Biol Chem. 1999 Sep 24;274(39):27343-6 ...
A Synaptonemal Complex Protein Promotes Homology-Independent Centromere Coupling Message Subject. (Your Name) has forwarded a ...
... complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and ... a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The ... Protein-protein interaction databases. Protein interaction database and analysis system. More...IntActi. P49452, 153 ... Centromere protein CAdd BLAST. 906. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. ...
... the centromere targeting region and the spindle targeting domain (Adams, 2001b). Inner centromere protein : Evolutionary ... Gene name - Inner centromere protein Synonyms - Cytological map position - 43A2--3 Function - signaling Keywords - chromatin ... Inner centromere protein : Biological Overview , Evolutionary Homologs , Regulation , Developmental Biology , Effects of ... EMS was chosen as a mutagen, with the aim of obtaining point mutations that specifically disrupt protein-protein interactions ( ...
CENP-B protein , centromere protein B , Major centromere autoantigen B , centromere autoantigen protein B , centromere protein ... Human Centromere Protein B (CENPB) Interaktionspartner * Given that CENP-B is the only centromere protein that binds centromere ... Centromere Protein B (CENPB) Antigen-Profil Protein Überblick This gene product is a highly conserved protein that facilitates ... anti-Centromere Protein B (CENPB) Antikörper. CENPB product is a highly conserved protein that facilitates centromere formation ...
Protein level used designations for Centromere Protein B (CENPB) ELISA Kits CENP-B protein , centromere protein B , Major ... Additionally we are shipping Centromere Protein B Antibodies (41) and Centromere Protein B Proteins (7) and many more products ... Centromere Protein B (CENPB) ELISA Kits. CENPB product is a highly conserved protein that facilitates centromere formation. ... More ELISA Kits for Centromere Protein B Interaction Partners. Mouse (Murine) Centromere Protein B (CENPB) interaction partners ...
Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads.. Earnshaw WC1, ... This suggests that while CENP-C may be confined to the outer centromere in the kinetochore region, CENP-B may be distributed ... 2) CENP-B is present at both active and inactive centromeres of stable dicentric autosomes. CENP-C is not detectable at the ... We have screened for the presence of two centromere autoantigens, CENP-B (80 kDa) and CENP-C (140 kDa) at the inactive ...
Centromere Protein O Antibody is available 2 times from supplier abbex at Gentaur.com shop ... Centromere Protein O Antibody. Centromere Protein O Antibody is available 2 times from Abbex labs abx111546 , Centromere ...
green fluorescent protein. INCENP. inner centromere protein. CENP-E. centromere protein E. ... 1997) Chromosomal proteins and cytokinesis: patterns of cleavage furrow formation and inner-centromere protein positioning in ... 1985) A family of centromere proteins is absent from the latent centromere of a stable isodicentric chromosome. Chromosoma ( ... A Dominant Mutant of Inner Centromere Protein (INCENP), a Chromosomal Protein, Disrupts Prometaphase Congression and ...
  • Efficacy of chimeric fusogenic proteins was evaluated by the evidence that the HAC, tagged with a drug-resistant gene and an EGFP gene, was transferred from CHO donor cells into human fibroblasts. (biomedcentral.com)
  • Retargeted MV-MMCT using chimeric H protein with scFvs succeeded in extending the cell spectrum for gene transfer via HAC vectors. (biomedcentral.com)
  • Here, we show that retargeting of microcell fusion by adding anti-Transferrin receptor (TfR) single chain antibodies (scFvs) to the extracellular C-terminus of the MV-H protein improves the efficiency of MV-MMCT to human fibroblasts which originally barely express both native MV receptors, and are therefore resistant to MV-MMCT. (biomedcentral.com)
  • The nascent synapsis generated by SYCP1 is stabilized through interaction with central element proteins SYCE1 and SYCE2 (By similarity). (icr.ac.uk)
  • Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy. (semanticscholar.org)
  • Application of Measles Virus (MV) fusogenic proteins to MMCT instead of polyethylene glycol (PEG) has partly solved this drawback, whereas the tropism of MV fusogenic proteins is restricted to human CD46- or SLAM-positive cells. (biomedcentral.com)
  • It was demonstrated that higher efficiency of microcell fusion was achieved in some human cells by means of microcells which expressed MV-derived fusion machinery, both the hemagglutinin (H) protein and fusion (F) protein, as compared to PEG-induced fusion. (biomedcentral.com)
  • Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. (wikipedia.org)
  • Since the nucleus is the largest cell organelle guiding the process of biological cell life, researchers have focused on seeking out the location(s) in the nucleus of the query protein so as to explore its function. (mdpi.com)
  • Previously it has been shown that the absence of non-essential kinetochore proteins of the Ctf19 complex weakens kinetochore-microtubule interaction, but whether this compromised interaction affects centromere-kinetochore positioning inside the nucleus is unknown. (duhnnae.com)
  • This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. (wikipedia.org)
  • In animal cells, Taxol disrupts microtubule formation by binding to microtubules and accelerating their assembly from the protein precursor, tubulin. (google.com)
  • Antinuclear autoantibody immunofluorescence performed for workup of possible inflammatory arthropathy showed a high titre cell cycle related nuclear speckled pattern, with subsequent confirmation by addressable laser bead immunoassay of the target antigen as an immunodominant epitope at the C-terminus of centromere protein F. (beds.ac.uk)
  • A chromatid is one of a pair of replicated DNA molecules still connected by a common centromere. (ups.edu)
  • Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity. (nature.com)
  • We unequivocally show that the SPB-centromere proximity and distances are not dependent on physical interactions between SPB and kinetochore components, but involve microtubule-dependent forces only. (duhnnae.com)
  • C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. (mcponline.org)
  • Several conserved proteins, including PRC1 and MKLP1, have been identified by cytology and genetics as C phase-specific microtubule-binding proteins that are required for midzone assembly and cytokinesis ( 7 ), but given the complexities of cytokinesis, others are likely to exist. (mcponline.org)
  • The nucleosomes of centromeres are characterized by a special H3-like histone (CenH3), which evolves rapidly and adaptively in Drosophila and Arabidopsis. (nih.gov)
  • CENH3 has a variable N-terminal and a more conserved C-terminal part, including the loop1 region of the histone fold domain, which is considered to be critical for centromere targeting. (deepdyve.com)
  • Except for LnCENH3, all recombinant CENH3 proteins targeted A. thaliana centromeres, but the more distantly related the heterologous protein is, the lower is the efficiency of targeting. (deepdyve.com)
  • Scientists from the Max Planck Institute of Immunobiology and Epigenetics in Freiburg have succeeded in demonstrating that the position, function and inheritance of the centromere are determined by the histone CenH3, a DNA packaging protein. (redorbit.com)
  • CenH3 arises exclusively in DNA regions at the centromere in various organisms. (redorbit.com)
  • Patrick Heun's research group at the Max Planck Institute of Immunobiology and Epigenetics and colleagues from the Helmholtz Research Center in Munich have now discovered that CenH3 alone is sufficient to trigger the formation of the centromere. (redorbit.com)
  • For their experiments, the researchers equipped the CenH3 histone with an artificially attached DNA binding domain so that the protein could bind to a DNA region where a centromere does not normally form. (redorbit.com)
  • The protein is able to recruit additional CenH3 proteins independently. (redorbit.com)
  • This ensures that sufficient CenH3 is available at the centromere after each cell division. (redorbit.com)
  • Otherwise, the available CenH3 proteins would be reduced by half after each cell division. (redorbit.com)
  • The insights into the central role of CenH3 for centromere identity could also prove important for medicine. (redorbit.com)
  • The nucleosomes of centromeres are characterized by a special H3-like histone CENH3 [ 30 ]. (hindawi.com)
  • In this study, we use chemical genetics to show that the protein kinase Mps1 regulates both aspects of the SAC. (pubmedcentralcanada.ca)
  • After Fas stimulation, Daxx is activated and plays its role of pro-apoptotic protein in activating the c-JUN-N-Terminal Kinase (JNK) pathway. (wikipedia.org)
  • In this paper we describe the molecular evolution of one centromere/kinetochore component, originally identified as the protein product of the Drosophila melanogaster gene l(1)zw10 , hereafter called DmZW10. (rupress.org)
  • A receptor, called the type IV coupling protein (T4CP), is positioned at the cytoplasmic entrance of the secretion channel to recognize specific plasmid-bound protein complexes and deliver them to the channel. (frontiersin.org)
  • Only those wells that contain Bovine Centromere protein U, MLF1IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (lifescience-market.com)
  • The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Centromere protein U, MLF1IP in the samples is then determined by comparing the O.D. of the samples to the standard curve. (lifescience-market.com)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. (nih.gov)
  • Owens TW, Gilmore AP, Streuli CH, Foster FM (2013) Inhibitor of Apoptosis Proteins: Promising Targets for Cancer Therapy. (omicsonline.org)
  • The Inhibitor of Apoptosis (IAP) proteins act downstream of a broad range of stimuli, such as cytokines and extracellular matrix interactions, to regulate cell survival, proliferation and migration. (omicsonline.org)
  • Since their discovery almost 20 years ago, the Inhibitor of Apoptosis (IAP) family of proteins have gathered growing interest as possible drug targets in a wide range of malignancies. (omicsonline.org)
  • In addition, anti-CENP-O antibody-positive sera were tested by Western blotting HeLa cell extracts to examine reactivity with the major centromere antigens. (fujita-hu.ac.jp)
  • Plants have regional centromeres, spanning several megabase pairs and are generally composed of species-specific satellite DNA interspersed with retrotransposons, predominantly Ty3- gyps [ 22 , 23 ], but may also contain several genes [ 24 , 25 ]. (hindawi.com)
  • The IAPs were first discovered in baculoviruses, where they were found to encode for proteins (cpIAP, OpIAP) able to inhibit apoptosis in the host cell [ 1 , 2 ]. (omicsonline.org)
  • Pachytene-FISH analysis of the native centromere organization allowed proposing the origin of PRO1 and PAT2 fragments. (hindawi.com)