Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Centrifugation, Density Gradient
A technique used to separate particles according to their densities in a continuous density gradient. The sample is usually mixed with a solution of known gradient materials and subjected to centrifugation. Each particle sediments to the position at which the gradient density is equal to its own. The range of the density gradient is usually greater than that of the sample particles. It is used in purifying biological materials such as proteins, nucleic acids, organelles, and cell types.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Electrophoresis, Polyacrylamide Gel
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Rats, Inbred Strains
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Chromatography, Ion Exchange
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Blood Specimen Collection
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Evaluation Studies as Topic
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Chemistry Techniques, Analytical
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Sodium Dodecyl Sulfate
Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Chromatography, High Pressure Liquid
Amino Acid Sequence
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Immature ERYTHROCYTES. In humans, these are ERYTHROID CELLS that have just undergone extrusion of their CELL NUCLEUS. They still contain some organelles that gradually decrease in number as the cells mature. RIBOSOMES are last to disappear. Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.
Electron Transport Complex IV
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Nucleic Acid Renaturation
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Electrophoresis, Agar Gel
Staining and Labeling
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Centrifugation fluids. The high density of the caesium ion makes solutions of caesium chloride, caesium sulfate, and caesium ...
BEA Westfalia separator Group, 2013, Chamber Bowl separator Berk, Z. (2013). Centrifugation. Food Process Engineering and ...
Centrifugation Methods. John Wiley & Sons, Mar 4, 2004, pp. 247-267. "Svedberg Lecture". Retrieved 2019-02-18. "Beckman ... Analytical ultracentrifugation Gas centrifuge Theodor Svedberg Differential centrifugation Buoyant density ultracentrifugation ...
Partial specific volume
Centrifugation". Biophysical Chemistry of Proteins. New York, Dordrecht, Heidelberg, London: Springer. pp. 237-249. doi:10.1007 ...
Centrifugation Methods. John Wiley & Sons, Mar 4, 2004, pp. 247-267. Frothingham, R (February 1999). "Centrifugation without a ... There are various types of centrifugation: Differential centrifugation, often used to separate certain organelles from whole ... Another potential hazard is the aerosolization of hazardous samples during centrifugation. To prevent contamination of the ... often used to isolate nucleic acids such as DNA Sucrose gradient centrifugation, often used to purify enveloped viruses and ...
"Basics of Centrifugation". Cole-Parmer. Retrieved 11 March 2012. "Plasmid DNA Separation: Fixed-Angle and Vertical Rotors in ... Centrifugation Methods. John Wiley & Sons, Mar 4, 2004, pp. 247-267. Vogel-Prandtl, Johanna Ludwig Prandtl: A Biographical ... Centrifugal force Centrifugation Clearing factor Honey extractor Hydroextractor Lamm equation Sedimentation coefficient ... Protocols for centrifugation typically specify the amount of acceleration to be applied to the sample, rather than specifying a ...
The gas centrifugation process uses a unique design that allows gas to constantly flow in and out of the centrifuge. Unlike ... "Basics of Centrifugation." Cole-Parmer Technical Lab. 14 Mar. 2008 "Gas Centrifuge Uranium Enrichment." Global Security.Org. 27 ... The output lines take these separations to other centrifuges to continue the centrifugation process. The process begins when ...
Solid bowl centrifuge
accessed 12 October 2013) Orris E. A, Eugene E.G. (1969). Centrifugation of Waste Sludges. Journal Water Pollution Control ...
Sedimentation Centrifugation Helmenstine, Todd. "What is decanting?". ThoughtCo. Retrieved 1 March 2018. "Separation and ...
By glycerol gradient centrifugation. By a DNA column. By an ion chromatography column. And also combinations of the above ...
For blood samples, these include centrifugation followed by examination of the buffy coat; mini anion-exchange/centrifugation; ... For other samples, such as spinal fluid, concentration techniques include centrifugation followed by examination of the ...
Centrifugation is the preferred method. Several preventives are available by prescription from a veterinarian to prevent dogs ...
Separates by centrifugation No. Yes. Yes Separates by decantation No. No. Yes ...
Soil salinity control
The extract is obtained by centrifugation. The salinity can more easily be measured, without centrifugation, in a 2:1 or 5:1 ...
The lysis-centrifugation method was introduced in 1917 by Mildred Clough, but it was rarely used in clinical practice until ... "History of Lysis-Centrifugation Blood Culture Methods". Murray, PR; Masur, H (2012). "Current approaches to the diagnosis of ... While lysis-centrifugation offers greater sensitivity than conventional blood culture methods, it is prone to contamination ... A technique called the lysis-centrifugation method can be used for improved isolation of slow-growing or fastidious organisms, ...
... this separation may be achieved by centrifugation. Precipitation by ammonium sulfate is a result of a reduction in solubility ...
Phytoliths must be concentrated using density centrifugation. Sodium polytungstate is a common substance used to aid in this ...
Continuous flow centrifugation: Two venous lines are used. This method requires slightly less blood volume out of the body at ... Discontinuous flow centrifugation: One venous catheter line is required. Typically, a 300 ml batch of blood is removed at a ...
Characterization of nanoparticles
Chromatography, centrifugation, and filtration techniques can be used to separate nanoparticles by size or other physical ... Mechanical separation techniques utilize membranes and/or centrifugation. Chemical separation techniques are liquid-liquid ...
Purification is accomplished by filtration, centrifugation, and bleaching. Carnauba wax can produce a glossy finish and as such ...
After centrifugation and washing, the starch is dried. Co-products account for 34% of wet-milled yield. In fact, 23% of corn ...
This is most easily done by centrifugation, which packs the denser blood cells and platelets to the bottom of the centrifuge ... Plasma is obtained by centrifugation before clotting occurs. The type of test required dictates what type of sample is used. A ...
Washed red blood cells
They can use centrifugation or centrifugation-free methods. The red cells can be re-suspended in saline or other types of ...
Isolation may be achieved by filtration or centrifugation. For some polymers it was demonstrated that thermoresponsive behavior ...
Similarly, so-called swim-up techniques apply a centrifugation step and then sperm is allowed to swim up into a medium, thus ... Density gradient centrifugation (in a continuous or discontinuous gradient) can concentrate semen samples with low ... However, use of sperm centrifugation is detrimental to the sperm viability and elicits production of reactive oxygen species. ... Several conventional techniques of centrifugation or swim-up. Newly applied methods such as flow cytometry expand the ...
Carr, Dr Steven M. "CsCl density-gradient centrifugation". www.mun.ca. Retrieved 2017-04-03. Martinez, Lluis. "Magnetic DNA ...
... generally carried out by centrifugation or ultra-centrifugation. If the product is biomass, then it is recovered for processing ... Ultra filtration is an alternative to the centrifugation. Cell disruption: If the desired product is intra cellular the cell ... The solid-liquid is separated by centrifugation or filtration and cell debris is discarded. Concentration of broth: The spent ...
This is done by filtration, centrifugation or dialysis. A method that does not require separation is the scintillation ...
The insoluble protein can be collected by centrifugation. One of the very effective ways for carrying out this process is the ...
Centrifugation | Sigma-Aldrich
Centrifugation Ergonomics - Eppendorf
second-centrifugation oil - International Olive Council
Centrifugation - LC Linked Data Service: Authorities and Vocabularies | Library of Congress
multiple-ejaculate resuspension and centrifugation | Taber's Medical Dictionary
multiple-ejaculate resuspension and centrifugation answers are found in the Tabers Medical Dictionary powered by Unbound ... "Multiple-ejaculate Resuspension and Centrifugation." Tabers Medical Dictionary, 24th ed., F.A. Davis Company, 2021. Tabers ... Multiple-ejaculate resuspension and centrifugation. In: Venes DD, ed. Tabers Medical Dictionary. F.A. Davis Company; 2021. ... multiple-ejaculate resuspension and centrifugation is a topic covered in the Tabers Medical Dictionary. To view the entire ...
Bio-Rad® Protein Expression and Purification Series: Centrifugation Purification Process | Sargent Welch
Centrifugation, Density Gradient | Profiles RNS
"Centrifugation, Density Gradient" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... This graph shows the total number of publications written about "Centrifugation, Density Gradient" by people in this website by ... Below are the most recent publications written about "Centrifugation, Density Gradient" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Centrifugation, Density Gradient". ...
Principles of Centrifugation - Beckman Coulter
Get your basic centrifugation operating questions answered and learn the fundamentals of separations using centrifugal force ... Principles of Centrifugation. You may have operated a centrifuge before. Or perhaps this is your first day on a new job at a ... We hope to provide an overview of basic centrifugation operating principles. We think youll be a better centrifuge operator if ...
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a) Analis Academy - Education session centrifugation. b) Training for best user practices can be organized in your or our ... BECKMAN COULTER Centrifugation & Ultracentrifugation. The guarantee of satisfaction throughout the life of your investment.. ... Advice on the choice of rotors / tubes / accessories and optimization of centrifugation parameters. - Our centrifuge product ... Analis offers the following services linked to centrifugation:. - Prevention Program - FRIP: Field Rotor Inspection Program. - ...
Stair-stepping and squats during centrifugation: feasibility, biomechanics, and fitness benefits. (Abstract and poster) | HSL
Stair-stepping and squats during centrifugation: feasibility, biomechanics, and fitness benefits. (Abstract and poster). Title ... Stair Stepping And Squats During Centrifugation Feasibility Biomechanics And Fitness Benefits. ... Stair-stepping and squats during centrifugation: feasibility, biomechanics, and fitness benefits. (Abstract and poster). ...
Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential
Density gradient centrifugation (DGC) and swim-up are useful techniques for separation of spermatozoa with longer telomeres. ... Density gradient centrifugation (DGC) and swim-up are useful techniques for separation of spermatozoa with longer telomeres. ... However, one of the disadvantages of this technique is sperm exposure to shear forces during centrifugation which is believed ... A. Comparison of absolute and B. Relative of STL among washed semen samples, density gradient centrifugation (DGC), and zeta- ...
Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro - Open access publications in the...
Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not. been ... Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro ... Brandt, Ylva and Morrell, Jane and Gonzalez Herrero, Raquel (2013). Single layer centrifugation-selected boar spermatozoa are ... METHODS: The semen was prepared either by SLC or by standard centrifugation (control) and used for in vitro. fertilization (IVF ...
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- Centrifugation, Density Gradient" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
- This graph shows the total number of publications written about "Centrifugation, Density Gradient" by people in this website by year, and whether "Centrifugation, Density Gradient" was a major or minor topic of these publications. (childrensmercy.org)
- Below are the most recent publications written about "Centrifugation, Density Gradient" by people in Profiles. (childrensmercy.org)
- Density gradient centrifugation (DGC) and swim-up are useful techniques for separation of spermatozoa with longer telomeres. (ijfs.ir)
- The density of DNA can be calculated using density gradient centrifugation. (news-medical.net)
- From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. (lu.se)
- Figure 1 Isolation of PBMCs and LIMCs from peripheral blood and liver biopsy specimens in the patients with AIH and CH-C by using density gradient centrifugation. (wjgnet.com)
- One day late in the night in our parasitology laboratory upon finishing density gradient centrifugation of Blastocystisculture, I took a layer of Blastocystisand placed over a slide and observed under 40 X objective. (isid.org)
Filtration or centrifugation1
- The immobilized enzymes can be easily separated from the reaction solution via simples filtration or centrifugation. (ncl.edu.tw)
- Ensure that the Amylose Resin drains completely after each spin (some older centrifuge models may require a longer centrifugation time). (zymoresearch.com)
- Centrifuge the supernatant / PEG-it mixture at 1500 × g for 30 minutes at 4 ° C. After centrifugation, the Lentivector particles may appear as a beige or white pellet at the bottom of the boat. (phrconference.org)
- Centrifuge the residual PEGit solution by centrifugation at 1500 × g for 5 minutes. (phrconference.org)
- With centrifugation, material is dewatered by centrifugal force - a force that draws particles in inertia away from the center of rotation. (mclanahan.com)
- To observe the effect of the serum on tomato puree viscosity, the pellet fraction containing the particles was spun out of the tomato purees by centrifugation so that the particle fraction and the serum could be assessed separately. (bl.uk)
- The principle of the centrifugation technique is to separate the particles suspended in liquid media under the influence of a centrifugal field. (renee-online.nl)
- Principles of centrifugation In a solution particles whose density is higher than that of the solvent sink (sediment) and particles that are lighter than it float to the top. (renee-online.nl)
- Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. (slu.se)
- The content of reactive oxygen species (ROS) will be analysed in semen samples from bulls of high and low fertility, and the effect of selecting robust spermatozoa by colloid centrifugation on their ROS-content will be investigated. (lantbruksforskning.se)
- Sperm preparation techniques, such as colloid centrifugation, that select the best quality spermatozoa could help to reduce harmful ROS-production and aid cryosurvival, thus contributing to improved cow fertility. (lantbruksforskning.se)
- In addition, the effect of selecting robust spermatozoa by colloid centrifugation on their ROS-content would be investigated. (lantbruksforskning.se)
- 2021. https://www.tabers.com/tabersonline/view/Tabers-Dictionary/766833/all/multiple_ejaculate_resuspension_and_centrifugation. (tabers.com)
- Centrifugation, the name given to separation applications which involve spinning around an axis to produce a centrifugal force, is a way to increase the magnitude of the gravitational field. (regiosteunpunt.nl)
- Liquid handling (pipettes, dispensers, automated LH workstations etc.) and centrifugation products, as well as plastic consumables such as Eppendorf test tubes and pipette tips. (eppendorf.com)
- The concentrator function enables the quick concentration of samples via vacuum (fast evaporation of the solvent) and centrifugation (prevents foaming over/sample loss) in the rotor. (eppendorf.com)
- We hope to provide an overview of basic centrifugation operating principles. (mybeckman.com.br)
- Principle of Centrifugation. (renee-online.nl)
- The protein fraction is concentrated by additional centrifugation. (fda.gov)
- Too high temperature, centrifugation or tumble can ruin details on the garment. (casall.com)
- Dewatering most often occurs through processes such as wet classification, centrifugation, filtration or similar liquid-solid separation processes. (mclanahan.com)
- 3. The apparatus according to claim 1, wherein said centrifugation baskets are fixed at the bottom at a shaft, at the lower end of which a pulley is fixed, and said shaft in inserted and supported in such a way to freely rotate by suitable bearings, within a ring, fixed to transverse elements, which are bridging provided between said parallel transport chains. (freepatentsonline.com)
- 6. The apparatus according to claim 1, wherein a tunnel is provided about part of the onward run of said transport chain supporting said centrifugation baskets, the inner walls of said tunnel collecting water ejected from the holes of said centrifugation baskets and to convey the same toward suitable lateral drainage. (freepatentsonline.com)
- Entrapment efficiency: Centrifugation of Nano-liposomes (0.5ml) was performed by using Amicon ultra filters (10kDa) at 13000 rpm for 15min. (eventscribe.com)
- It is used to obtain the olive oil - in certified organically grown quality - by mechanical processes such as pressing and centrifugation. (robygge.se)
- Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet. (qiagen.com)
- Intracellular distribution and reactivity of radiolabeled chromium in Chinese-hamster cells was assessed by fractional centrifugation and a combination of anion exchange and ion pair high performance liquid chromatography. (cdc.gov)
- Any of several methods-gaseous diffusion, gas centrifugation, liquid thermal diffusion-can be employed to separate and concentrate the fissile uranium-235 isotope into several grades, from low-enrichment (2 to 3 percent uranium-235) to fully enriched (97 to 99 percent uranium-235). (britannica.com)
- Increase centrifugation time and speed. (zymoresearch.com)
- Thirty mL water is added to the flask, followed by shaking, centrifugation and two hexane rinses. (fda.gov)
- 12. The apparatus according to claim 1, wherein said apparatus comprises water jets for cleaning said centrifugation baskets, provided under the apparatus before the end of the return run. (freepatentsonline.com)
- Harvesting takes place by centrifugation of the mix - water/microalgae. (plexiglas.de)
- Oil extracted continuously in a second centrifugation line from olive paste that has already been centrifuged once. (internationaloliveoil.org)
- Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. (qiagen.com)
- *Trainings a) Analis Academy - Education session centrifugation b) Training for best user practices can be organized in your or our facilities. (analis.be)
- 4. The apparatus according to claim 1, wherein said means for conferring to said centrifugation baskets a fast rotary motion about their own axis comprises a pair of belts, realizing a closed loop about two pulleys, provided side-by-side and rotating according to the same direction by two further belts, operated by two motors rotating according to the same direction. (freepatentsonline.com)
- Stair-stepping and squats during centrifugation: feasibility, biomechanics, and fitness benefits. (mit.edu)