Cementogenesis: The formation of DENTAL CEMENTUM, a bone-like material that covers the root of the tooth.Pseudomonadaceae: A family of gram-negative bacteria usually found in soil or water and including many plant pathogens and a few animal pathogens.beta-MSH: An 18-amino acid peptide that is the C-terminal fragment of gamma-lipotropin which is the N-terminal fragment of BETA-LIPOTROPIN. Beta-MSH is shown to regulate skin pigmentation, steroid production, and feeding.Cellulomonas: A genus of aerobic or facultatively anaerobic BACTERIA, in the family Cellulomonadaceae. It is found in the SOIL and capable of hydrolyzing CELLULOSE.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Xylans: Polysaccharides consisting of xylose units.Glycoside HydrolasesCellulose: A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.Pseudomonas fluorescens: A species of nonpathogenic fluorescent bacteria found in feces, sewage, soil, and water, and which liquefy gelatin.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Cellvibrio: A genus of aerobic, gram-negative, motile, slightly curved, rod-shaped bacteria. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Endo-1,4-beta Xylanases: Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.Xylosidases: A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.Cryptococcus: A mitosporic Tremellales fungal genus whose species usually have a capsule and do not form pseudomycellium. Teleomorphs include Filobasidiella and Fidobasidium.Xylan Endo-1,3-beta-Xylosidase: A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.beta-Mannosidase: An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.Lotus: A plant genus of the family FABACEAE. This genus was formerly known as Tetragonolobus. The common name of lotus is also used for NYMPHAEA and NELUMBO.Mannosidases: Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.United States Food and Drug Administration: An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.Mannans: Polysaccharides consisting of mannose units.Research Report: Detailed account or statement or formal record of data resulting from empirical inquiry.Collectins: A class of C-type lectins that target the carbohydrate structures found on invading pathogens. Binding of collectins to microorganisms results in their agglutination and enhanced clearance. Collectins form trimers that may assemble into larger oligomers. Each collectin polypeptide chain consists of four regions: a relatively short N-terminal region, a collagen-like region, an alpha-helical coiled-coil region, and carbohydrate-binding region.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Mannose-Binding Lectin: A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.Germanium: A rare metal element with a blue-gray appearance and atomic symbol Ge, atomic number 32, and atomic weight 72.63.Biometric Identification: A method of differentiating individuals based on the analysis of qualitative or quantitative biological traits or patterns. This process which has applications in forensics and identity theft prevention includes DNA profiles or DNA fingerprints, hand fingerprints, automated facial recognition, iris scan, hand geometry, retinal scan, vascular patterns, automated voice pattern recognition, and ultrasound of fingers.Radio Frequency Identification Device: Machine readable patient or equipment identification device using radio frequency from 125 kHz to 5.8 Ghz.Doping in Sports: Illegitimate use of substances for a desired effect in competitive sports. It includes humans and animals.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Brief Psychiatric Rating Scale: A scale comprising 18 symptom constructs chosen to represent relatively independent dimensions of manifest psychopathology. The initial intended use was to provide more efficient assessment of treatment response in clinical psychopharmacology research; however, the scale was readily adapted to other uses. (From Hersen, M. and Bellack, A.S., Dictionary of Behavioral Assessment Techniques, p. 87)Bacterial Proteins: Proteins found in any species of bacterium.Cytoskeletal Proteins: Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Cellobiose: A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.DextrinsClostridium thermocellum: A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.Kinetics: The rate dynamics in chemical or physical systems.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Volvariella: A genus of edible mushrooms in the family Pluteaceae. They have pink gills and a volva at the stem base, and species can be confused with those of the poisonous genus AMANITA.Glycosyltransferases: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.Glycosides: Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.Desert Climate: A type of climate characterized by insufficient moisture to support appreciable plant life. It is a climate of extreme aridity, usually of extreme heat, and of negligible rainfall. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Metagenomics: The genomic analysis of assemblages of organisms.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Actinobacteria: Class of BACTERIA with diverse morphological properties. Strains of Actinobacteria show greater than 80% 16S rDNA/rRNA sequence similarity among each other and also the presence of certain signature nucleotides. (Stackebrandt E. et al, Int. J. Syst. Bacteriol. (1997) 47:479-491)

A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions. (1/33)

Hydrolysis of the plant cell wall polysaccharides cellulose and xylan requires the synergistic interaction of a repertoire of extracellular enzymes. Recently, evidence has emerged that anaerobic bacteria can synthesize high levels of periplasmic xylanases which may be involved in the hydrolysis of small xylo-oligosaccharides absorbed by the micro-organism. Cellvibrio mixtus, a saprophytic aerobic soil bacterium that is highly active against plant cell wall polysaccharides, was shown to express internal xylanase activity when cultured on media containing xylan or glucose as sole carbon source. A genomic library of C. mixtus DNA, constructed in lambdaZAPII, was screened for xylanase activity. The nucleotide sequence of the genomic insert from a xylanase-positive clone that expressed intracellular xylanase activity in Escherichia coli revealed an ORF of 1137 bp (xynC), encoding a polypeptide with a deduced M(r) of 43413, defined as xylanase C (XylC). Probing a gene library of Pseudomonas fluorescens subsp. cellulosa with C. mixtus xynC identified a xynC homologue (designated xynG) encoding XylG; XylG and xynG were 67% and 63% identical to the corresponding C. mixtus sequences, respectively. Both XylC and XylG exhibit extensive sequence identity with family 10 xylanases, particularly with non-modular enzymes, and gene deletion studies on xynC supported the suggestion that they are single-domain xylanases. Purified recombinant XylC had an M(r) of 41000, and displayed biochemical properties typical of family 10 polysaccharidases. However, unlike previously characterized xylanases, XylC was particularly sensitive to proteolytic inactivation by pancreatic proteinases and was thermolabile. C. mixtus was grown to late-exponential phase in the presence of glucose or xylan and the cytoplasmic, periplasmic and cell envelope fractions were probed with anti-XylC antibodies. The results showed that XylC was absent from the culture media but was predominantly present in the periplasm of C. mixtus cells grown on glucose, xylan, CM-cellulose or Avicel. These data suggest that C. mixtus can express non-modular internal xylanases whose potential roles in the hydrolysis of plant cell wall components are discussed.  (+info)

The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan. (2/33)

The microbial degradation of xylan is a key biological process. Hardwood 4-O-methyl-D-glucuronoxylans are extensively decorated with 4-O-methyl-D-glucuronic acid, which is cleaved from the polysaccharides by alpha-glucuronidases. In this report we describe the primary structures of the alpha-glucuronidase from Cellvibrio mixtus (C. mixtus GlcA67A) and the alpha-glucuronidase from Pseudomonas cellulosa (P. cellulosa GlcA67A) and characterize P. cellulosa GlcA67A. The primary structures of C. mixtus GlcA67A and P. cellulosa GlcA67A, which are 76% identical, exhibit similarities with alpha-glucuronidases in glycoside hydrolase family 67. The membrane-associated pseudomonad alpha-glucuronidase released 4-O-methyl-D-glucuronic acid from 4-O-methyl-D-glucuronoxylooligosaccharides but not from 4-O-methyl-D-glucuronoxylan. We propose that the role of the glucuronidase, in combination with cell-associated xylanases, is to hydrolyze decorated xylooligosaccharides, generated by extracellular hemicellulases, to xylose and 4-O-methyl-D-glucuronic acid, enabling the pseudomonad to preferentially utilize the sugars derived from these polymers.  (+info)

Convergent evolution sheds light on the anti-beta -elimination mechanism common to family 1 and 10 polysaccharide lyases. (3/33)

Enzyme-catalyzed beta-elimination of sugar uronic acids, exemplified by the degradation of plant cell wall pectins, plays an important role in a wide spectrum of biological processes ranging from the recycling of plant biomass through to pathogen virulence. The three-dimensional crystal structure of the catalytic module of a "family PL-10" polysaccharide lyase, Pel10Acm from Cellvibrio japonicus, solved at a resolution of 1.3 A, reveals a new polysaccharide lyase fold and is the first example of a polygalacturonic acid lyase that does not exhibit the "parallel beta-helix" topology. The "Michaelis" complex of an inactive mutant in association with the substrate trigalacturonate/Ca2+ reveals the catalytic machinery harnessed by this polygalacturonate lyase, which displays a stunning resemblance, presumably through convergent evolution, to the tetragalacturonic acid complex observed for a structurally unrelated polygalacturonate lyase from family PL-1. Common coordination of the -1 and +1 subsite saccharide carboxylate groups by a protein-liganded Ca2+ ion, the positioning of an arginine catalytic base in close proximity to the alpha-carbon hydrogen and numerous other conserved enzyme-substrate interactions, considered in light of mutagenesis data for both families, suggest a generic polysaccharide anti-beta-elimination mechanism.  (+info)

The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants. (4/33)

alpha-Glucuronidases are key components of the ensemble of enzymes that degrade the plant cell wall. They hydrolyze the alpha1,2-glycosidic bond between 4-O-methyl-d-glucuronic acid (4-O-MeGlcA) and the xylan or xylooligosaccharide backbone. Here we report the crystal structure of an inactive mutant (E292A) of the alpha-glucuronidase, GlcA67A, from Cellvibrio japonicus in complex with its substrate. The data show that the 4-O-methyl group of the substrate is accommodated within a hydrophobic sheath flanked by Val-210 and Trp-160, whereas the carboxylate moiety is located within a positively charged region of the substrate-binding pocket. The carboxylate side chains of Glu-393 and Asp-365, on the "beta-face" of 4-O-MeGlcA, form hydrogen bonds with a water molecule that is perfectly positioned to mount a nucleophilic attack at the anomeric carbon of the target glycosidic bond, providing further support for the view that, singly or together, these amino acids function as the catalytic base. The capacity of reaction products and product analogues to inhibit GlcA67A shows that the 4-O-methyl group, the carboxylate, and the xylose sugar of aldobiouronic acid all play an important role in substrate binding. Site-directed mutagenesis informed by the crystal structure of enzyme-ligand complexes was used to probe the importance of highly conserved residues at the active site of GlcA67A. The biochemical properties of K288A, R325A, and K360A show that a constellation of three basic amino acids (Lys-288, Arg-325, and Lys-360) plays a critical role in binding the carboxylate moiety of 4-O-MeGlcA. Disruption of the apolar nature of the pocket created by Val-210 (V210N and V210S) has a detrimental effect on substrate binding, although the reduction in affinity is not reflected by an inability to accommodate the 4-O-methyl group. Replacing the two tryptophan residues that stack against the sugar rings of the substrate with alanine (W160A and W543A) greatly reduced activity.  (+info)

Reclassification of 'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. (5/33)

'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 has been demonstrated by a polyphasic taxonomic approach to be a member of the genus Cellvibrio. 16S rDNA sequence analysis suggests that this is the only genus that could accept this specimen. The sequence is 95.5% similar to that of Cellvibrio mixtus subsp. mixtus ACM 2601T (the type strain of the type species of the genus), which is its closest relation. The genomic DNA G + C content was determined to be 53.3 mol%, which is similar to the values obtained for the validly described Cellvibrio species. DNA-DNA hybridization experiments have shown that strain NCIMB 10462T (= NCDO 2697T) represents a novel species; therefore, it is proposed that it be designated as the type strain of the novel species Cellvibrio japonicus sp. nov. This study also used 16S rDNA analysis, DNA-DNA hybridization experiments and phenotypic testing to revive the species Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. C. vulgaris NCIMB 8633T (=LMG 2848T) and C. fulvus NCIMB 8634T (=LMG 2847T) are the proposed type strains.  (+info)

Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp. nov., Cellvibrio fibrivorans sp. nov. and Cellvibrio gandavensis sp. nov. (6/33)

Thirty-one cellulolytic bacterial isolates from soils that were phenotypically very similar and phylogenetically highly related to Cellvibrio strains were further characterized using a polyphasic taxonomic approach. By using repetitive extragenic palindromic DNA-PCR fingerprinting, six different fingerprints could be recognized among the isolates. Representative strains and four reference strains of the genus Cellvibrio were used for DNA-DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70% DNA binding. One group was formed by three isolates and Cellvibrio vulgaris LMG 2848T and a second group consisted of Cellvibrio mixtus strains ACM 2601T and ACM 2603. Two isolates and Cellvibrio fulvus LMG 2847T constituted single-member groups. For the remaining groups, three novel species are proposed: Cellvibrio fibrivorans sp. nov. (six strains, type strain LMG 18561T =ACM 5172T), Cellvibrio ostraviensis sp. nov. (eight strains, type strain LMG 19434T =ACM 5173T) and Cellvibrio gandavensis sp. nov. (12 strains, type strain LMG 18551T =ACM 5174T). The novel Cellvibrio species could be differentiated from each other and from C. mixtus, C. vulgaris and C. fulvus on the basis of phenotypic features, their fatty acid compositions and the G + C content of their DNA.  (+info)

Parallel induction of D-arabitol and D-sorbitol dehydrogenases. (7/33)

Scolnick, Edward M. (Harvard Medical School, Boston, Mass.) and Edmund C. C. Lin. Parallel induction of d-arabitol and d-sorbitol dehydrogenases. J. Bacteriol. 84:631-637. 1962.-Two inducible diphosphopyridine nucleotide-linked dehydrogenases are described in a bacterium isolated from the soil, Cellvibrio polyoltrophicus ATCC 14774. The first enzyme catalyzes the dehydrogenation of d-arabitol to d-xylulose and d-mannitol to d-fructose. The data suggest that in vivo this enzyme has the dual function of the utilization of both of these polyhydric alcohols. The second enzyme was found to act only on d-sorbitol, converting it to d-fructose. Evidence for its physiological function as a d-sorbitol dehydrogenase is also given. Both of these enzymes were found to be induced in parallel by any of the three polyhydric alcohols, d-arabitol, d-mannitol, and d-sorbitol. A common stereoconfiguration of the inducers for these enzymes is suggested. The parallel evolution of substrate specificity and inducer specificity is discussed with respect to the functional advantage that such a selective process might offer.  (+info)

The mechanisms by which family 10 glycoside hydrolases bind decorated substrates. (8/33)

Endo-beta-1,4-xylanases (xylanases), which cleave beta-1,4 glycosidic bonds in the xylan backbone, are important components of the repertoire of enzymes that catalyze plant cell wall degradation. The mechanism by which these enzymes are able to hydrolyze a range of decorated xylans remains unclear. Here we reveal the three-dimensional structure, determined by x-ray crystallography, and the catalytic properties of the Cellvibrio mixtus enzyme Xyn10B (CmXyn10B), the most active GH10 xylanase described to date. The crystal structure of the enzyme in complex with xylopentaose reveals that at the +1 subsite the xylose moiety is sandwiched between hydrophobic residues, which is likely to mediate tighter binding than in other GH10 xylanases. The crystal structure of the xylanase in complex with a range of decorated xylooligosaccharides reveals how this enzyme is able to hydrolyze substituted xylan. Solvent exposure of the O-2 groups of xylose at the +4, +3, +1, and -3 subsites may allow accommodation of the alpha-1,2-linked 4-O-methyl-d-glucuronic acid side chain in glucuronoxylan at these locations. Furthermore, the uronic acid makes hydrogen bonds and hydrophobic interactions with the enzyme at the +1 subsite, indicating that the sugar decorations in glucuronoxylan are targeted to this proximal aglycone binding site. Accommodation of 3'-linked l-arabinofuranoside decorations is observed in the -2 subsite and could, most likely, be tolerated when bound to xylosides in -3 and +4. A notable feature of the binding mode of decorated substrates is the way in which the subsite specificities are tailored both to prevent the formation of "dead-end" reaction products and to facilitate synergy with the xylan degradation-accessory enzymes such as alpha-glucuronidase. The data described in this report and in the accompanying paper indicate that the complementarity in the binding of decorated substrates between the glycone and aglycone regions appears to be a conserved feature of GH10 xylanases.  (+info)

The microbial degradation of the plant cell wall is a pivotal biological process that is of increasing industrial significance. One of the major plant structural polysaccharides is mannan, a beta-1,4-linked d-mannose polymer, which is hydrolyzed by endo- and exo-acting mannanases. The mechanisms by which the exo-acting enzymes target the chain ends of mannan and how galactose decorations influence activity are poorly understood. Here we report the crystal structure and biochemical properties of CjMan26C, a Cellvibrio japonicus GH26 mannanase. The exo-acting enzyme releases the disaccharide mannobiose from the nonreducing end of mannan and mannooligosaccharides, harnessing four mannose-binding subsites extending from -2 to +2. The structure of CjMan26C is very similar to that of the endo-acting C. japonicus mannanase CjMan26A. The exo-activity displayed by CjMan26C, however, reflects a subtle change in surface topography in which a four-residue extension of surface loop creates a steric block at ...
Buy high purity recombinant enzyme endo-1-4-beta-Mannanase Cellvibrio japonicus for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Purchase high purity recombinant enzyme endo-1-4-beta-Xylanase (Cellvibrio japonicas) for research, biochemical enzyme assays in vitro diagnostic analysis.
ID A0A1U9NAH2_9GAMM Unreviewed; 119 AA. AC A0A1U9NAH2; DT 07-JUN-2017, integrated into UniProtKB/TrEMBL. DT 07-JUN-2017, sequence version 1. DT 11-DEC-2019, entry version 9. DE RecName: Full=Cell division protein FtsL {ECO:0000256,HAMAP-Rule:MF_00910}; GN Name=ftsL {ECO:0000256,HAMAP-Rule:MF_00910}; GN ORFNames=B0D95_10625 {ECO:0000313,EMBL:AQT60495.1}; OS Cellvibrio sp. PSBB023. OC Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; OC Cellvibrionaceae; Cellvibrio; unclassified Cellvibrio. OX NCBI_TaxID=1945512 {ECO:0000313,EMBL:AQT60495.1, ECO:0000313,Proteomes:UP000189646}; RN [1] {ECO:0000313,EMBL:AQT60495.1, ECO:0000313,Proteomes:UP000189646} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PSBB023 {ECO:0000313,EMBL:AQT60495.1, RC ECO:0000313,Proteomes:UP000189646}; RA Peterson S.W.; RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Essential cell division protein. May link together the CC upstream cell division proteins, which are ...
Enzymes of family GH31 are retaining α-glycosidases, as was first demonstrated by a combination of polarimetric and reducing sugar measurement [2]. GH31 enzymes (except for the α-glucan lyases) are believed to follow the classical Koshland double-displacement mechanism. [3] This has been strongly supported by labeling of the catalytic nucleophile of several GH31 enzymes using conduritol B epoxide [4], with early examples including rabbit intestinal sucrase/isomaltase [5] and human lysosomal α-glucosidase [6]. Later studies on an α-glucosidase from Aspergillus niger [7], an α-xylosidase from Escherichia coli [8], YihQ sulfoquinovosidase from Escherichia coli [1], and an α-xylosidase from Cellvibrio japonicus [9] used the more reliable 5-fluoroglycosyl fluoride trapping reagents, which form catalytically competent intermediates. Subsequently, retention of the anomeric configuration was directly observed by H-1 NMR during the hydrolysis of a natural xylogluco-oligosaccharide substrate by C. ...
Modules of approx. 130 residues. A module that is conserved in three Cellvibrio xylan-degrading enzymes binds to xylan and the interaction is calcium dependent, while a module from a Cellvibrio mannanase binds to decorated soluble mannans and mannooligosaccharides. A module in a Phanerochaete chrysosporium galactan 1,3-β-galactosidase binds to β-galactan ...
Two intracellular enzymes, cellobiose phosphorylase (CBP) and cellodextrin phosphorylase (CDP) are involved in the phosphorolytic pathway in cellulose degradation. Those enzymes are considered to be useful in syntheses of oligosaccharides because the reactions are reversible. CBP from Cellvibrio gilvus and Clostridium thermocellum YM4, and CDP from C. thermocellum YM4 were cloned and over-expressed in Escherichia coli. All the three enzymes showed ordered bi bi mechanism. However the orders of the substrate binding of the CBPs were different. It was found that CBP from C. gilvus strictly recognized the hydroxyl groups at positions β-1, 3, and 4 of the acceptor molecule in the reverse reaction. On the other hand, the recognition of the hydroxyl groups at positions 2 and 6 was not so strict. Three branched β-1, 4-glucosyl trisaccharides were synthesized by using the reverse reaction of C. gilvus CBP. A new substrate inhibition pattern, competitive substrate inhibition, was also found in the ...
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Aktiivsemateks õhulämmastiku sidujateks mullas on aeroobsed asotobakterid(Azotobacter chroococcum, Azotobacter vinelandii, Azotobacter aglis jt). Nad on polümorfsed ja seotud lämmastiku (nitritite, nitraatide, aminohapete jt.) puudumisel omastavad õhulämmastikku. Osa seotud lämmastikust eritatakse eksosmoosil ümbritsevasse keskkonda kas amiinohapetena või ammoniaagina. Energeetilise materjalina võivad nad kasutada nii mono-, di- kui ka mõningaid teisi polüsahhariide ja mitmeid alkohole, orgaanilisi happeid, sh. ka aromaatseid (näiteks bensoehape). Nad ei kasuta tselluloosi, kuid tselluloosirikas materjal (põhk, põhurikas sõnnik) intensiivistab nende paljunemist. Põhjendatav on see sellega, et tselluloos lõhustatakse mullas tsellulolüütiliste bakterite (Cellvibrio spp., Cellulomonas spp., Cellfalcicula spp. jt) poolt lihtsamateks süsivesikuteks, mida saavad kasutada asotobakterid ...
Genome sequencing of Catenovulum agarivorans YM01T reveals 15 open-reading frames (ORFs) encoding various agarases. In this study, extracellular proteins of YM01T were precipitated by ammonium sulfate and separated by one-dimensional gel electrophoresis. The results of in-gel agarase activity assay and mass spectrometry analysis revealed that the protein, YM01-3, was an agarase with the most evident agarolytic activity. Agarase YM01-3, encoded by the YM01-3 gene, consisted of 420 amino acids with a calculated molecular mass of 46.9 kDa and contained a glycoside hydrolase family 16 β-agarase module followed by a RICIN superfamily in the C-terminal region. The YM01-3 gene was cloned and expressed in Escherichia coli. The recombinant agarase, YM01-3, showed optimum activity at pH 6.0 and 60 °C and had a Km of 3.78 mg mL−1 for agarose and a Vmax of 1.14 × 104 U mg−1. YM01-3 hydrolyzed the β-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products.
Looking for Glycone? Find out information about Glycone. A large important class of sugar derivatives in which the sugar is combined with a nonsugar. In their cyclic forms, monosaccharides possess one carbon atom... Explanation of Glycone
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
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The development of sustainable technologies for plant cell wall degradation greatly depends on enzymes with hydrolytic activities against carbohydrates. The waste by-products of agricultural cereals are important biomass sources because they contain large amounts of saccharides. Achieving efficient debranching and depolymerization are two important objectives for increasing the utilization of such renewable bioresources. GH51 α-l-arabinofuranosidases are important in biomass pretreatment because they act synergistically with other enzymes during hemicellulose hydrolysis. A GH51 α-l-arabinofuranosidase from Talaromyces leycettanus JCM12802 was heterologously expressed in Pichia pastoris GS115 and characterized. The recombinant α-l-arabinofuranosidase, TlAbf51, showed an optimum temperature and pH of 55-60 °C and 3.5-4.0, respectively, and remained stable at 50 °C and pH 3.0-9.0. TlAbf51 showed a higher catalytic efficiency (5712 mM−1 s−1) than most fungal α-l-arabinofuranosidases towards the
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I wanted to take the tree and everything else down yesterday. DH said to wait until Jan 1st. Sigh. We have the same debate every year. I really, really want to tidy up and take everything down today but I feel like a grinch! When do you - page 2
Celebrate the grace of those born under a year of the Rabbit with this lovable character. Designed by Hiroshi Yoshii and crafted in pink crystal, it will be a treasured keepsake. Decoration object. Not a toy. Not suitable for children under 15.
Cladanthus mixtus, commonly known as the Moroccan chamomile, is a Mediterranean species of flowering plant in the Asteraceae family. The plant is often considered a weed but is used to gather oils that are useful for calming people down and soothing the skin. The species oils change in composition depending on the area which it occupies. Cladanthus mixtus is mostly found in North Africa (Morocco, Algeria, Libya and Tunisia). This species has also been known to occur on the northern side of the Mediterranean (Spain, Italy, Greece, France and Albania). The species is sparingly naturalized in a few widely scattered locations in North America. The Moroccan Chamomile likes moist rich soil to grow and thrive in, but it also has the ability to grow in soils that are more salty around the Mediterranean Sea. Cladanthus mixtus does not need extreme amounts of water as it can retain water because of its thicker cuticle, allowing the species to hold water for longer durations. Cladanthus mixtus can ...
The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal structure of MZ0003, which reveals that residues comprising the catalytic triad differ from previously-characterized fungal homologs, and resolves three large loop regions that are unique to this bacterial sub-clade. The catalytic triad of the bacterial CE15, which includes Asp 332 as its third member, closely resembles that of family 1 carbohydrate esterases (CE1), despite the overall lower structural similarity with members of this family ...
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A. tumefaciens is used in the construction of genetically engineered plants, as it is able to transfer DNA to plant hosts. Knowledge of the mechanisms of DNA transfer and the genes required will aid in the understanding of this process. Manipulation of glycoside hydrolases may increase transformation and widen the host range of the bacterium.... ...
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Glycosides are classified based on the following: 1) Chemical nature of aglycone part 2)Therapeutic activity of aglycone part. 3)Type of linkage between glycone and aglycone part. REF:PHARMACOGNOSY:NEERALI PRAKASHAN PUBLICATIONS. 34 edition, page-167 ...
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Morning, yall. Been pretty productive for the last two days, which feels really good. I decided we werent going to really decorate for the holidays, but after pulling everything out, I changed my mind. One of my girlfriends borrowed all of my tasteful decorations to decorate her store, so Ive dec...
Scope and method of study: To study the plant cell wall degradation process and changes in overall physiology during the growth of A. nidulans on sorghum stover at proteomic and genomic level, A. nidulans was grown on sorghum stover under solid-state culture conditions for 1, 2, 3, 5, 7 and 14 days. Semi-quantitative extracellular proteome analysis (1-D PAGE LC-MS/MS), whole genome microarray analysis, scanning and transmission electron microscopy, along with qualitative and quantitative analysis of extracellular hydrolytic enzyme activities, and analysis of the breakdown products by enzymes was used to study sorghum cell wall degradation by A. nidulans.Findings and conclusions: Based on analysis of chitin content, A. nidulans grew to be 4-5% of the total biomass in the culture. A hyphal mat developed on the surface of the sorghum by day one and as seen by scanning electron microscopy the hyphae enmeshed the sorghum particles by day 5. A total of 294 extracellular proteins were identified with ...
Pectin lyases (PNLs) are important enzymes that are involved in plant cell wall degradation during the infection process. Colletotrichum is a diverse genus of fungi, which allows the study of the evolution of PNLs and their possible role in pathogen-host interactions and lifestyle adaptations. The phylogenetic reconstruction of PNLs from Colletotrichum and analysis of selection pressures showed the formation of protein lineages by groups of species with different selection pressures and specific patterns. The analysis of positive selection at individual sites using different methods allowed for the identification of three codons with evidence of positive selection in the oligosaccharide-binding region and two codons on the antiparallel sheet, which may influence the interaction with the substrate ...
The warbling vireo, Vireo gilvus, is known for its unique song, which the Cornell Lab of Ornithology describes as "a rapid jumble of rising and falling notes." This description also fits the featured writing by Neil de la Flor, which creates a poetic warbling, constantly changing and varying notes, tone, mood, and approach. The warbling of de la Flors poem, however, is not the lyric beauty of the stereotypical poems inspired by birdsong. This poem does not have the lines, rhythm, and most importantly song expected of lyricism. Instead of the perfect arrival of a beautiful spring, we read: "Meta enjoyed sex and a lack of context". The food is not the scrumptious, juicy berries found in the bushes at the side of the dirt road in the forest at the edge of the meadow, but burgers from McDonalds ...
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Dont wait for happiness to happen, create it! Heres the secret to changing your life for the better. Claim your free 10 chapter e-book. Your email address will not be passed on to third parties ...
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Xylan, a major component of plant hemicellulose, is a complex, highly branched heteropolymer. Endo-β-1,4-xylanases (EC 3.2.1.8) are glycosidases that cleave the internal β-1,4-xylosidic bonds of the main chain of xylan and produce unbranched or branched xylooligosaccharides (1). Due to the complexity of the xylan structure, endo-β-1,4-xylanases are diverse in sequences, structures, hydrolytic activities, and enzymatic properties. They are distributed in glycoside hydrolase (GH) families 5, 7, 8, 10, 11, and 43. Among endo-β-1,4-xylanases, those from GH family 10 (GH10) and GH11 have been studied in more detail. GH10 xylanases have an (α/β)8 barrel structure (2), while GH11 xylanases have a β-jelly roll structure (3). Despite their different structures, both enzyme families adopt the catalytic mechanism of anomeric retention to release xylooligosaccharides from xylan (2, 3).. The endo-β-1,4-xylanases in GH family 8 reported so far include XylY from Bacillus sp. strain KK-1 (4), PhXyl from ...
In molecular biology, Glycoside hydrolase family 14 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. Glycoside hydrolase family 14 CAZY GH_14 comprises enzymes with only one known activity; beta-amylase (EC 3.2.1.2). A Glu residue has been proposed as a catalytic residue, but it is not known if it is the nucleophile or the proton donor. Beta-amylase is an enzyme that hydrolyzes 1,4-alpha-glucosidic linkages in starch-type polysaccharide substrates so as to remove successive maltose units from the non-reducing ends of ...
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1GWL: Promiscuity in Ligand-Binding: The Three-Dimensional Structure of a Piromyces Carbohydrate-Binding Module,Cbm29-2,in Complex with Cello- and Mannohexaose
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Nakabo, T., 2002. Fishes of Japan with pictorial keys to the species, English edition I. Tokai University Press, Japan, pp v-866. (Ref. 41299 ...
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ニホンザリガニ(Cambaroides japonicus)の小河川における生息環境の選好性と効率的な調査手法について(予報) (2008 ...
article{9b276fde-3141-41ff-ba06-611da1a392ac, abstract = {,p,Lipases and glycoside hydrolases have large similarities concerning reaction mechanisms. Acyl-enzyme intermediates are formed during lipase-catalyzed reactions and in an analogous way, retaining glycoside hydrolases form glycosyl-enzyme intermediates during catalysis. In both cases, the covalent enzyme intermediates can react with water or other nucleophiles containing hydroxyl groups. Simple alcohols are accepted as nucleophiles by both types of enzymes. Lipases are used very successfully in synthesis applications due to their efficiency in catalyzing reversed hydrolysis and transesterification reactions. On the other hand, synthesis applications of glycoside hydrolases are much less developed. Here, important similarities and differences between the enzyme groups are reviewed and approaches to reach high synthesis yields are discussed. Useful strategies include the use of low-water media, high nucleophile concentrations, as well as ...
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3VUS: The structure of the deacetylase domain of Escherichia coli PgaB, an enzyme required for biofilm formation: a circularly permuted member of the carbohydrate esterase 4 family
Cellulomonas flavigena ATCC ® 482D-5™ Designation: Genomic DNA from Cellulomonas flavigena strain NRS 134 TypeStrain=True Application:
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Ouch. There is an all-too-obvious visual difference for something that should be an implementation detail, and the possibility of users encountering both styles in the same session doesnt help either.. Of course it would be easy to just blame the GNOME design team - after all, while the metacity-1 theme format used by the window manager is hugely different from CSS as used by GTK+, it would still be possible to create a more consistent look from the lowest common denominator of both formats. But if the goal is to have both types of decorations match as closely as possible, using different themes and expecting an already overworked team to put additional work into papering over the differences is hardly the best solution.. Did I mention that the metacity-1 format is also plainly unpleasant?. So from GNOME 3.16 on, the window manager theme will be gone. Instead, window manager decorations will use the same theme information GTK+ uses for its client-side decorations. As you would expect, both ...
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TY - JOUR. T1 - Asp271 is critical for substrate interaction with the surface binding site in β-agarase A from Zobellia galactanivorans. AU - Wilkens, Casper. AU - Tiwari, Manish K.. AU - Webb, Helen. AU - Jam, Murielle. AU - Czjzek, Mirjam. AU - Svensson, Birte. PY - 2019. Y1 - 2019. N2 - In the marine environment agar degradation is assured by bacteria that contain large agarolytic systems with enzymes acting in various endo- and exo-modes. Agarase A (AgaA) is an endo-glycoside hydrolase of family 16 considered to initiate degradation of agarose. Agaro-oligosaccharide binding at a unique surface binding site (SBS) in AgaA from Zobellia galactanivorans was investigated by computational methods in conjunction with a structure/sequence guided approach of site-directed mutagenesis probed by surface plasmon resonance binding analysis of agaro-oligosaccharides of DP 4-10. The crystal structure has shown that agaro-octaose interacts via H-bonds and aromatic stacking along 7 subsites (L through R) of ...
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As a rule, about 1% of genes in a given genome encode glycoside hydrolases and their homologues. On the basis of sequence similarity they have been grouped into more than ninety GH families during the last 15 years. The GH97 family has been established very recently and initially included only 18 bacterial proteins. However, the evolutionary relationship of the genes encoding proteins of this family remains unclear, as well as their distribution among main groups of the living organisms. The extensive search of the current databases allowed us to double the number of GH97 family proteins. Five subfamilies were distinguished on the basis of pairwise sequence comparison and phylogenetic analysis. Iterative sequence analysis revealed the relationship of the GH97 family with the GH27, GH31, and GH36 families of glycosidases, which belong to the α-galactosidase superfamily, as well as a more distant relationship with some other glycosidase families (GH13 and GH20). The results of this study show an
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Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Cobalt atom in PDB 1h41: Pseudomonas Cellulosa E292A Alpha-D-Glucuronidase Mutant Complexed With Aldotriuronic Acid
A baby room, or nursery, is a room that will be remembered long after the baby has grown into adulthood. Decorating the nursery is, in one way, planning ahead for how the months and years after birth will be reminisced. Also, the decorations in a nursery will be some of the earliest things a newborn will set eyes upon, and parents rightly want to make those decorations … [Read more...] about How to Decorate a Baby Room ...
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cellulosa and Cellvibrio mixtus". Biochem. J. 312 (1): 39-48. PMC 1136224 . PMID 7492333. Fanutti C, Ponyi T, Black GW, ... Cellvibrio mixtus endoglucanase 5A contains two CBM6 domains, the CBM6 domain at the C-terminus displays distinct ligand ...
Ccl3; TC# 2.A.46.1.6), or as many as 14 TMSs (i.e., BenE of Cellvibrio gilvus; TC# 2.A.46.1.4). BenE family members exhibit ...
2002). "Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold". Nat Struct Biol. 9 (9): 665-8 ... The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove ...
"Structural and enzymatic characterisation of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in ...
Taxonomy of the psychrophile Flavobacterium frigidarium and the mesophile Cellvibrio japonicus, and comparative analyses of ...
Cellvibrio, Mesophilobacter, Pseudomonas (the type genus), Rhizobacter, Rugamonas, and Serpens. The family Azotobacteriaceae ...
Cellvibrio MeSH B03.440.400.425.292 --- comamonadaceae MeSH B03.440.400.425.292.150 --- Comamonas MeSH B03.440.400.425.292.150. ... Cellvibrio MeSH B03.660.250.580.590 --- Pseudomonas MeSH B03.660.250.580.590.050 --- Pseudomonas aeruginosa MeSH B03.660. ...
... is a genus of Gammaproteobacteria. The cells are slender curved rods. Cellvibrio is (like all Proteobacteria) Gram- ... Springer, New York, 2005, Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria Cellvibrio J.P. Euzéby: List of ...
Cellvibrio is a genus of Gammaproteobacteria. The cells are slender curved rods. Cellvibrio is (like all Proteobacteria) Gram- ... Springer, New York, 2005, Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria Cellvibrio J.P. Euzéby: List of ...
sp,B3PKE3,SYR_CELJU Arginine--tRNA ligase OS=Cellvibrio japonicus (strain Ueda107) GN=argS PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Cellvibrio › ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa). ,p>This subsection of the ,a href="http://www ...
sp,B3PF20,EX7S_CELJU Exodeoxyribonuclease 7 small subunit OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=xseB PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Cellvibrio › ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa). ,p>This subsection of the ,a href="http://www ...
Cellvibrio sp. BR. Polaribacter atrinae. Polaribacter vadi. Rheinheimera sp. KL1. Cellvibrio mixtus. Saccharophagus degradans ( ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)Imported. ,p>Information which has been ... tr,B3PIY5,B3PIY5_CELJU Beta-galactosidase, putative, bgl2A OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=bgl2A PE=3 SV= ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Cellvibrio › ...
Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with Glc-4Glc-3Glc-4Glc. *DOI: 10.2210/ ... The Crystal Structure of the Family 6 Carbohydrate Binding Module from Cellvibrio Mixtus Endoglucanase 5A in Complex with ...
Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus.. ... Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus.. ... Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus.. ... unravel the intricate repertoire of plant cell wall-degrading enzymes synthesized by the saprophytic soil bacterium Cellvibrio ...
Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp. nov., Cellvibrio fibrivorans sp. nov. and ... One group was formed by three isolates and Cellvibrio vulgaris LMG 2848T and a second group consisted of Cellvibrio mixtus ... Two isolates and Cellvibrio fulvus LMG 2847T constituted single-member groups. For the remaining groups, three novel species ... 12 strains, type strain LMG 18551T =ACM 5174T). The novel Cellvibrio species could be differentiated from each other and from C ...
... and functional analysis of a lytic polysaccharide monooxygenase important for efficient utilization of chitin in Cellvibrio ...
Arabinogalactan endo-beta-1,4-galactanase is an enzyme with system name arabinogalactan 4-beta-D-galactanohydrolase. The enzyme specifically hydrolyses (1->4)-beta-D-gala
The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification ... Dedication: This article is dedicated to Cellvibrio japonicus vanguard Prof. Harry J. Gilbert on the occasion of his retirement ... The Gram-negative bacterium, Cellvibrio japonicus Ueda107 (formerly, Pseudomonas fluorescens subsp. cellulosa) has emerged as a ... Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus. J Bacteriol. ...
Cellvibrio japonicus Ueda107. Summary assignment statistics followed by sortable table of detailed assignments with number ... Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Cellvibrio; Cellvibrio japonicus; Cellvibrio ... Cellvibrio japonicus Ueda107 superfamily assignments. Taxonomy. Taxonomy browser entry. ...
The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification ... From: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent ...
Cellvibrio japonicas) for research, biochemical enzyme assays in vitro diagnostic analysis. ... These enzymes, in combination with either Cellvibrio mixtus or Neocallimastix patriciarum β -xylanase, were used to produce ... High purity recombinant endo-1,4-β-Xylanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro ... Recombinant. Catalytic domain from Cellvibrio japonicus. In 3.2 M ammonium sulphate.. Supplied at ~ 500 U/mL. ...
Buy high purity recombinant enzyme endo-1-4-beta-Mannanase Cellvibrio japonicus for use in research, biochemical enzyme assays ... High purity recombinant endo-1,4 β-Mannanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro ... From Cellvibrio japonicus.. In 3.2 M ammonium sulphate.. Supplied at ~ 5,000 U/mL. ...
Regulation of the xylan-degrading apparatus of Cellvibrio japonicus by a novel two-component system. *Lookup NU author(s) ... of two genes that modulate the expression of xylan side chain-degrading enzymes in the saprophytic bacterium Cellvibrio ...
The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the ... Here we report the crystal structure and biochemical properties of CjMan26C, a Cellvibrio japonicus GH26 mannanase. The exo- ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Cellvibrio japonicus Fragment: RESIDUES 197-328 Details: MANNAN SPECIFIC CARBOHYDRATE BINDING MODULE Gene Name(s): man5C ...
1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., ... Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp. nov., Cellvibrio fibrivorans sp. nov. and ... Cellvibrio fulvus NCIMB 8634 16S ribosomal RNA gene, partial sequence. ATCC 12120 T ... Electronmicroscopic observations on the degradation of cellulose fibres by Cellvibrio fulvus and Sporocytophaga myxococcoides ...
Cellvibrio japonicus Ueda107. Mutation(s): 0 Gene Names: agd31B, CJA_3248. EC: 2.4.1.161. ...
Cellvibrio japonicus (strain Ueda107). Mutation(s): 0 Gene Names: agd31B. EC: 2.4.1.161. ...
Native full-length Gal53 from Cellvibrio japonicus. In 3.2 M ammonium sulphate. Specific activity: ~ 100 U/mg (40oC, pH 8.0 on ... Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. EC 3.2.1.89 CAZy Family ... endo-1,4-β-Galactanase (Cellvibrio japonicus) [ME-GALCJ] - High purity recombinant endo-1,4-beta-Galactanase ( ... endo-1,4-β-Galactanase (Cellvibrio japonicus). Price(USD):$282.49. *Ask Promo Code: Equl SKU. Product Name. UOM. Specification ...
B3PK56 (RL15_CELJU) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa). 50S ribosomal protein L15 ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa) ...
B3PK33 (RS7_CELJU) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa). 30S ribosomal protein S7 ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa) ...
B3PK38 (RL4_CELJU) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa). 50S ribosomal protein L4 ... Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subspcellulosa) ...
  • To unravel the intricate repertoire of plant cell wall-degrading enzymes synthesized by the saprophytic soil bacterium Cellvibrio japonicus, we sequenced and analyzed its genome, which predicts that the bacterium contains the complete repertoire of enzymes required to degrade plant cell wall and storage polysaccharides. (jcvi.org)
  • Here we report the biochemical properties of the GH43 enzymes of a saprophytic soil bacterium, Cellvibrio japonicus, and a human colonic symbiont, Bacteroides thetaiotaomicron. (ubc.ca)
  • For the remaining groups, three novel species are proposed: Cellvibrio fibrivorans sp. (nih.gov)
  • Cellvibrio is a genus of Gammaproteobacteria. (wikipedia.org)
  • Representative strains and four reference strains of the genus Cellvibrio were used for DNA-DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70% DNA binding. (nih.gov)
  • Analysis of the same samples-incubated with fungi Cellvibrio japonicus , Streptomyces cellulosae, and Trichoderma reseei- showed that the more complex carbon in fine pores is not more stable - that is, it is at least as easily decomposed as the simpler forms of C found in coarse pores. (pnnl.gov)