AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation ScienceHealth Care
CellulaseCellulasesCelluloseTrichodermaCellulose 1,4-beta-CellobiosidaseCellobioseCarboxymethylcellulose SodiumHypocreaGlycoside Hydrolasesbeta-GlucosidaseClostridium thermocellumMitosporic FungiDextrinsPolyporaceaeCellulosomesClostridium cellulolyticumClostridiumEndo-1,4-beta XylanasesGlucan 1,4-beta-GlucosidaseGlucosidasesXylansHydrolysisIsopteraTylenchidaAspergillus nigerLigninMolecular Sequence DataSporangiaNeocallimastixActinomycetalesBiomassClostridium cellulovoransPanicumHydrogen-Ion ConcentrationAmino Acid SequenceGossypiumPerseaGlucan 1,3-beta-GlucosidaseGene Expression Regulation, FungalGlucansBiofuelsFungal Proteinsbeta-MannosidaseFibrobacterEndo-1,3(4)-beta-GlucanasePolygalacturonaseSubstrate SpecificityAscomycotaNematodaSchizophyllumCloning, MolecularEnzyme StabilityLactosePenicilliumBacillusPhanerochaeteFungiBacterial ProteinsFermentationGram-Negative Anaerobic Straight, Curved, and Helical RodsTemperature
Cellulase
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Cellulases
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
Cellulose
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Trichoderma
A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.
Cellulose 1,4-beta-Cellobiosidase
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
Cellobiose
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
Carboxymethylcellulose Sodium
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
Hypocrea
A genus of fungus in the family Hypocreaceae, order HYPOCREALES. Anamorphs include TRICHODERMA.
Glycoside Hydrolases
beta-Glucosidase
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
Clostridium thermocellum
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
Mitosporic Fungi
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
Dextrins
Polyporaceae
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
Cellulosomes
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
Clostridium cellulolyticum
A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.
Clostridium
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
Endo-1,4-beta Xylanases
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
Glucan 1,4-beta-Glucosidase
An exocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages of 1,4-beta-D-glucans resulting in successive removal of GLUCOSE units.
Glucosidases
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
Xylans
Polysaccharides consisting of xylose units.
Hydrolysis
The process of cleaving a chemical compound by the addition of a molecule of water.
Isoptera
An order of insects, restricted mostly to the tropics, containing at least eight families. A few species occur in temperate regions of North America.
Tylenchida
An order of nematodes consisting of many species which are plant parasites. Female worms lay eggs that hatch either in soil or in the host plant.
Aspergillus niger
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
Lignin
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Sporangia
A structure found in plants, fungi, and algae, that produces and contains spores.
Neocallimastix
A genus of fungi in the family Neocallimasticaceae, order NEOCALLIMASTIGALES. They contain polyflagellate zoospores and grow on a range of simple and complex carbohydrates in the rumen of sheep and cattle.
Actinomycetales
An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.
Biomass
Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.
Clostridium cellulovorans
A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.
Panicum
A plant genus of the family POACEAE. The seed is one of the EDIBLE GRAINS used in millet cereals and in feed for birds and livestock (ANIMAL FEED). It contains diosgenin (SAPONINS).
Hydrogen-Ion Concentration
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Gossypium
A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.
Persea
A plant genus in the LAURACEAE family. The tree, Persea americana Mill., is known for the Avocado fruit, the food of commerce.
Glucan 1,3-beta-Glucosidase
An exocellulase with specificity for 1,3-beta-D-glucasidic linkages. It catalyzes hydrolysis of beta-D-glucose units from the non-reducing ends of 1,3-beta-D-glucans, releasing GLUCOSE.
Gene Expression Regulation, Fungal
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
Glucans
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
Biofuels
Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).
Fungal Proteins
Proteins found in any species of fungus.
beta-Mannosidase
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
Fibrobacter
A genus of gram-negative, anaerobic bacteria in the family Fibrobacteraceae, isolated from the human GASTROINTESTINAL TRACT.
Endo-1,3(4)-beta-Glucanase
An endocellulase with specificity for the hydrolysis of 1,3- or 1,4-linkages in beta-D-glucans. This enzyme specifically acts on sites where reducing glucose residues are substituted at the 3 position.
Polygalacturonase
A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.
Substrate Specificity
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Ascomycota
A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.
Nematoda
A class of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.
Schizophyllum
A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Enzyme Stability
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Lactose
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
Penicillium
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
Bacillus
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
Phanerochaete
A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.
Fungi
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Bacterial Proteins
Proteins found in any species of bacterium.
Fermentation
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Gram-Negative Anaerobic Straight, Curved, and Helical Rods
A group of anaerobic, rod-shaped bacteria that show up as pink (negative) when treated by the Gram-staining method.
Temperature
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain. (1/122)

The mechanisms by which cellulolytic enzymes and enzyme complexes in Ruminococcus spp. bind to cellulose are not fully understood. The product of the newly isolated cellulase gene endB from Ruminococcus flavefaciens 17 was purified as a His-tagged product after expression in Escherichia coli and found to be able to bind directly to crystalline cellulose. The ability to bind cellulose is shown to be associated with a novel cellulose-binding module (CBM) located within a region of 200 amino acids that is unrelated to known protein sequences. EndB (808 amino acids) also contains a catalytic domain belonging to glycoside hydrolase family 44 and a C-terminal dockerin-like domain. Purified EndB is also shown to bind specifically via its dockerin domain to a polypeptide of ca. 130 kDa present among supernatant proteins from Avicel-grown R. flavefaciens that attach to cellulose. The protein to which EndB attaches is a strong candidate for the scaffolding component of a cellulosome-like multienzyme complex recently identified in this species (S.-Y. Ding et al., J. Bacteriol. 183:1945-1953, 2001). It is concluded that binding of EndB to cellulose may occur both through its own CBM and potentially also through its involvement in a cellulosome complex.  (+info)

Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977. (2/122)

The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.  (+info)

Termite gut symbiotic archaezoa are becoming living metabolic fossils. (3/122)

Over the course of several million years, the eukaryotic gut symbionts of lower termites have become adapted to a cellulolytic environment. Up to now it has been believed that they produce nutriments using their own cellulolytic enzymes for the benefit of their termite host. However, we have now isolated two endoglucanases with similar apparent molecular masses of approximately 36 kDa from the not yet culturable symbiotic Archaezoa living in the hindgut of the most primitive Australian termite, Mastotermes darwiniensis. The N-terminal sequences of these cellulases exhibited significant homology to cellulases of termite origin, which belong to glycosyl hydrolase family 9. The corresponding genes were detected not in the mRNA pool of the flagellates but in the salivary glands of M. darwiniensis. This showed that cellulases isolated from the flagellate cells originated from the termite host. By use of a PCR-based approach, DNAs encoding cellulases belonging to glycosyl hydrolase family 45 were obtained from micromanipulated nuclei of the flagellates Koruga bonita and Deltotrichonympha nana. These results indicated that the intestinal flagellates of M. darwiniensis take up the termite's cellulases from gut contents. K. bonita and D. nana possess at least their own endoglucanase genes, which are still expressed, but without significant enzyme activity in the nutritive vacuole. These findings give the impression that the gut Archaezoa are heading toward a secondary loss of their own endoglucanases and that they use exclusively termite cellulases.  (+info)

Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture. (4/122)

The cellulolytic bacterium Ruminococcus albus 8 adheres tightly to cellulose, but the molecular biology underpinning this process is not well characterized. Subtractive enrichment procedures were used to isolate mutants of R. albus 8 that are defective in adhesion to cellulose. Adhesion of the mutant strains was reduced 50% compared to that observed with the wild-type strain, and cellulose solubilization was also shown to be slower in these mutant strains, suggesting that bacterial adhesion and cellulose solubilization are inextricably linked. Two-dimensional polyacrylamide gel electrophoresis showed that all three mutants studied were impaired in the production of two high-molecular-mass, cell-bound polypeptides when they were cultured with either cellobiose or cellulose. The identities of these proteins were determined by a combination of mass spectrometry methods and genome sequence data for R. albus 8. One of the polypeptides is a family 9 glycoside hydrolase (Cel9B), and the other is a family 48 glycoside hydrolase (Cel48A). Both Cel9B and Cel48A possess a modular architecture, Cel9B possesses features characteristic of the B(2) (or theme D) group of family 9 glycoside hydrolases, and Cel48A is structurally similar to the processive endocellulases CelF and CelS from Clostridium cellulolyticum and Clostridium thermocellum, respectively. Both Cel9B and Cel48A could be recovered by cellulose affinity procedures, but neither Cel9B nor Cel48A contains a dockerin, suggesting that these polypeptides are retained on the bacterial cell surface, and recovery by cellulose affinity procedures did not involve a clostridium-like cellulosome complex. Instead, both proteins possess a single copy of a novel X module with an unknown function at the C terminus. Such X modules are also present in several other R. albus glycoside hydrolases and are phylogentically distinct from the fibronectin III-like and X modules identified so far in other cellulolytic bacteria.  (+info)

Dietary lignins are precursors of mammalian lignans in rats. (5/122)

The mammalian lignans enterolactone (ENL) and enterodiol, commonly found in human plasma and urine, are phytoestrogens that may contribute to the prevention of breast cancer and coronary heart disease. They are formed by the conversion of dietary precursors such as secoisolariciresinol and matairesinol lignans by the colonic microflora. The identification of lignins, cell-wall polymers structurally related to lignans, as precursors of mammalian lignans is reported here for the first time. In study 1, rats were fed rye or wheat bran (15% diet) for 5 d. Untreated brans and brans extracted with solvents to remove lignans were compared. ENL was estimated in urine samples collected for 24 h by time-resolved fluoroimmunoassay. ENL urinary excretion was reduced from 18.6 to 5.3 nmol/d (n=8; P<0.001) when lignans were removed from rye bran and from 30.5 to 6.2 nmol/d (P<0.001) when they were removed from wheat bran. These results suggest that lignins, embedded in the cell wall and retained in the bran during solvent extraction, account for 26-32% of the ENL formed from cereal brans. In study 2, rats were fed a deuterated synthetic lignin (0.2% diet) together with wheat bran (15%) for 3 d. The detection of deuterated ENL by LC-tandem MS in urine (20 nmol/d) clearly confirms the conversion of lignin into mammalian lignans. More research is warranted to determine the bioavailability of lignins in the human diet.  (+info)

Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei) Cel7B catalytic module. (6/122)

The catalytic module of Hypocrea jecorina (previously Trichoderma reesei) Cel7B was homologously expressed by transformation of strain QM9414. Post-translational modifications in purified Cel7B preparations were analysed by enzymatic digestions, high performance chromatography, mass spectrometry and site-directed mutagenesis. Of the five potential sites found in the wild-type enzyme, only Asn56 and Asn182 were found to be N-glycosylated. GlcNAc(2)Man(5) was identified as the predominant N-glycan, although lesser amounts of GlcNAc(2)Man(7) and glycans carrying a mannophosphodiester bond were also detected. Repartition of neutral and charged glycan structures over the two glycosylation sites mainly accounts for the observed microheterogeneity of the protein. However, partial deamidation of Asn259 and a partially occupied O-glycosylation site give rise to further complexity in enzyme preparations.  (+info)

Fungal cell wall chitinases and glucanases. (7/122)

The fungal cell wall is a complex structure composed of chitin, glucans and other polymers, and there is evidence of extensive cross-linking between these components. The wall structure is highly dynamic, changing constantly during cell division, growth and morphogenesis. Hydrolytic enzymes, closely associated with the cell wall, have been implicated in the maintenance of wall plasticity and may have roles during branching and cross-linking of polymers. Most fungal cell wall hydrolases identified to date have chitinase or glucanase activity and this short article reviews the apparent functions of these enzymes in unicellular and filamentous fungi, and the mechanisms that regulate enzyme activity in yeasts.  (+info)

Structural, thermodynamic, and kinetic analyses of tetrahydrooxazine-derived inhibitors bound to beta-glucosidases. (8/122)

The understanding of transition state mimicry in glycoside hydrolysis is increasingly important both in the quest for novel specific therapeutic agents and for the deduction of enzyme function and mechanism. To aid comprehension, inhibitors can be characterized through kinetic, thermodynamic, and structural dissection to build an "inhibition profile." Here we dissect the binding of a tetrahydrooxazine inhibitor and its derivatives, which display Ki values around 500 nm. X-ray structures with both a beta-glucosidase, at 2 A resolution, and an endoglucanase at atomic (approximately 1 A) resolution reveal similar interactions between the tetrahydrooxazine inhibitor and both enzymes. Kinetic analyses reveal the pH dependence of kcat/Km and 1/Ki with both enzyme systems, and isothermal titration calorimetry unveils the enthalpic and entropic contributions to beta-glucosidase inhibition. The pH dependence of enzyme activity mirrored that of 1/Ki in both enzymes, unlike the cases of isofagomine and 1-deoxynojirimycin that have been characterized previously. Calorimetric dissection reveals a large favorable enthalpy that is partially offset by an unfavorable entropy upon binding. In terms of the similar profile for the pH dependence of 1/Ki and the pH dependence of kcat/Km, the significant enthalpy of binding when compared with other glycosidase inhibitors, and the tight binding at the optimal pH of the enzymes tested, tetrahydrooxazine and its derivatives are a significantly better class of glycosidase inhibitor than previously assumed.  (+info)

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Trp22, Trp24, and Tyr8 play a pivotal role in the binding of the family 10 cellulose-binding module from Pseudomonas xylanase A...Trp22, Trp24, and Tyr8 play a pivotal role in the binding of the family 10 cellulose-binding module from Pseudomonas xylanase A...
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Deconstruction Review of Fringe, Episode 16, Season 2, Olivia. In the Lab. With the Revolver « Cordial DeconstructionDeconstruction Review of Fringe, Episode 16, Season 2, Olivia. In the Lab. With the Revolver « Cordial Deconstruction
As usual, an episode synopsis can be found over at Scotts Polite Dissent. Im Mentioning Cytotoxins Just for the Search Engine Hits Epidermal blistering and tissue necrosis do indeed match the symptoms from cytotoxins, and so this section is designed solely to increase my search engine hits for this Deconstruction review of Fringe. While Im…
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Deconstruction of an Article on Automobile Hacking « Cordial DeconstructionDeconstruction of an Article on Automobile Hacking « Cordial Deconstruction
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Deconstruction - MMO BitsDeconstruction - MMO Bits
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While Youre Away: Avi wet the bed
It is certainly different to be at moms as opposed to Erins- I felt it the most today. My mom was gone today shopping for a few hours, so Emily was doing dishes, and helping out... I think she was a bit overwhelmed by it all, so I got up to help out with Avi a bit more than normal- Emily said thank you- I was up again this evening, because it was getting late- past Avis bed time- mom was just making dinner- Avi needed to start her probiotics, so I got up to give her them- Erin happened to be over and said I should lay down..the difference of understanding bed rest- and what possible affects could be of delivering early (the saying thank you vs. the lay down). Emily had also forgotten to get me lunch... I grabbed some fruit to tide me over. I felt like it is harder to stay down when left alone with Avi here- partly because if I get all the way down on the ground with her, I get rashed from the cat-carpet, and partly because Avi likes to travel up and down the steps. I am further along- 29 ...
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Deconstruction | Joyce GoldsteinDeconstruction | Joyce Goldstein
Deconstruction - I wrote this for the January February 2010 Food Arts Magazine It all started rather innocently. A reporter from the Chicago Tribune, Christopher Borrelli, called me for a story he was writing, to identify the Ten Worst Dining Trends of the Last Decade. He interviewed chefs, food professionals and writers. There were…
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The Deconstruction of White Society Shifts Into High Gear - PaulCraigRoberts.org
The Deconstruction of White Society Shifts Into High Gear Cambridge to assign white academics an ethnic minority mentor to combat racism and assist
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➤ Essay on Shawshank deconstruction for brooks parole sequence ✍ Brooks parole sequence is particularly evocative and compelling. A variety of techniques is used to shape this particularly emotive and tragic scene.
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specified in the pattern • You can ignore any keys that are not included in keys • If **rest is specified in the pattern, nil is passed instead • In such case, you must return all key-value pairs class Time VALID_KEYS = %i(year month ...) def deconstruct_keys(keys) if keys (VALID_KEYS & keys).each_with_object({}) do ,k, h, h[k] = send(k) end else {year: year, month: month, ...} end end end now = Time.now case now in year: # Calls now.deconstruct_keys([:year]), # receives {year: 2019} end ...
https://speakerdeck.com/k_tsj/pattern-matching-new-feature-in-ruby-2-dot-7?slide=38
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Deconstruction: Saccharification - ABPDUDeconstruction: Saccharification - ABPDU
Saccharification and pretreatment are integrated operations in biochemical process of converting cellulose and hemicellulose to fermentable sugars.
http://abpdu.lbl.gov/capabilities/deconstruction/saccharification/
Press releases for 4/18/2012
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Practical Fragments: MELK part 2: fragment deconstructionPractical Fragments: MELK part 2: fragment deconstruction
More surprisingly, removing the phenyl group from compound 8 produced a molecule with greater affinity and ligand efficiency than the initial compound 2! The researchers determined the crystal structure of this (compound 9) bound to MELK and found that, in contrast to the other molecules, it binds in the DFG-in conformation. The isoquinoline hinge binder actually binds in a similar manner as it does for its DFG-out binding cousins, its just the back pocket that is cut off. The researchers speculate that the DFG-in conformation of the protein may be lower energy, giving the edge to compounds that bind to this state. Whether or not this is the case, it is certainly another reminder of the remarkable plasticity of proteins ...
http://practicalfragments.blogspot.com/2015/02/melk-part-2-fragment-deconstruction.html
Thoughts on deconstruction and community | Community FragmentsThoughts on deconstruction and community | Community Fragments
So, to set up our undoubtedly engaging discussion about Derrida and the politics of community, I hereby post another experpt from my paper for Precarity course. Its not related directly to Derridas politics of the impossible friendship, but does go into the enduring non-individuation of the political movement. I argued in my essay that it…
https://communityfragments.wordpress.com/2010/03/12/thoughts-on-deconstruction-and-community/
Philly Decon - Philadelphia Community CorpsPhilly Decon - Philadelphia Community Corps
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MicrocircuitDB: Deconstruction of cortical evoked potentials generated by subthalamic DBS (Kumaravelu et al 2018)MicrocircuitDB: Deconstruction of cortical evoked potentials generated by subthalamic DBS (Kumaravelu et al 2018)
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https://senselab.med.yale.edu/MicroCircuitDB/ShowModel?model=244262&file=/cEP_stndbs_4.5hz/naf_tcr.mod
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Breaking: VMware Announces Intent to Aquire Avi Networks | BRBreaking: VMware Announces Intent to Aquire Avi Networks | BR
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Seth & Avi in Massage from Seth MenachemSeth & Avi in Massage from Seth Menachem
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Cocktail R-Evolution Molecular Gastronomy Kit by Molecule-R - Miscellaneous Brands
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http://www.chilledtoperfection.com/miscellaneous-brands/cocktail-r-evolution-molecular_1530230.aspx
How to draw PACH « TanglePatterns.comHow to draw PACH « TanglePatterns.com
Pach is a great Monday tangle from Judy Okawa. Judy shared her Infinity tangle with us this summer and shes also contributed a String that introduced us to the Japanese art of kintsugi. Do check out Judys String for more on that very interesting subject.. Pach is oh-so-easy and it offers so many opportunities for embellishing to personalize your own version.. Judy writes,. I recently saw a picture of a paper chain, the type that we used to make at Christmas time with strips of colored construction paper, formed into rings - The chain formed an interesting abstract pattern, so I wanted to try to deconstruct it, and Pach (for Paper Chain) is what I came up with…. Note that the small curved line added in Step 4 is drawn under, so as to complete the ring on the opposite side.. In my Pach explorations I first used Step 1 as the paired rice shapes Judy demonstrates. Then I remembered how the Zentangle®-original Zenith starts out and I used that method, shown here.. ...
https://tanglepatterns.com/2016/12/how-to-draw-pach.html?replytocom=862903
Modular origami - PolypompholyxModular origami - Polypompholyx
These are so beautiful, thanks for sharing! Unfortunately, I have been tearing my hair out trying to figure out how to make a few of these designs, specifically the triangular tetrahedrons and the rhomboid/kite-like octahedron. Ive searched the internet and Ive been to the library to check out several origami books, but I cannot seem to find a way to make them! Ive even tried to deconstruct them on paper to try and see how the units fit together, but that is a mess best left forgotten. In any case, heres a url that has screenshots of the models that Im having trouble with, if you could help clarify how to make them I would be most appreciative!. https://imgur.com/a/Yv1WsLo. The first one I designated the kite-like octahedron, the second the simple triangular tetrahedron, and the third the complex triangular tetrahedron.. ...
https://www.polypompholyx.com/2017/01/modularorigami/
Are Atheists looking in the wrong place? | Rational Response SquadAre Atheists looking in the wrong place? | Rational Response Squad
I have been debating theists for 11 years now online on various websites. Now while others here are willing to take the time to deconstruct what you have posted, I am not one of those. I am going to simply cut to the chase and address ALL concepts of non material thinking beings, not just yours. I see your particular pet god claim as a mere naked assertion like all god claims in human history.. WHY? Not what? BUT WHY do YOU feel the need to insert a thinking who into the gap?. I dont understand what you mean by this.. Does it take Posiden the ocean god to allow or make a hurricane? Does it take Thor to make lightening? Or could it be, just like all humans, even prior to the invention of your personal myth, that humans merely like the idea of having a super hero? There is a word for that anthropomorphism.. Read my post on the different Gods.. If you can accept that the Egyptian sun god was never real, despite that ancient culture believing it falsely for 3,000 years, what makes you think you ...
https://www.rationalresponders.com/forum/32648
TutorialsTutorials
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https://www.onedesigns.com/tutorials
Decon | LANGHOMES
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What is an exvangelical?What is an exvangelical?
What is an exvangelical? What does it mean to deconstruct your faith? How should I respond when people label themselves as exvangelicals?
https://www.gotquestions.blog/exvangelical.html
ICS Active Defense and Incident Response | National Initiative for Cybersecurity Careers and StudiesICS Active Defense and Incident Response | National Initiative for Cybersecurity Careers and Studies
ICS515: ICS Active Defense and Incident Response will help you deconstruct ICS cyber attacks, leverage an active defense to identify and counter threats in your ICS, and use incident response procedures to maintain the safety and reliability of operations.
https://niccs.us-cert.gov/training/search/sans-institute/ics-active-defense-and-incident-response
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Medical Imaging & Large Systems - SanminaMedical Imaging & Large Systems - Sanmina
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Sola Scriptura 4 - Canon and History
The thread of deconstruction I have in mind today is a tangled one indeed. Im not sure how well I can express it one post, but Ill do my best. Put simply, many of the ways the Bible is discussed among those today who hold to some variation of sola scriptura simply dont reflect the…
https://faithandfood.morizot.net/2009/08/20/sola-scriptura-4-canon-and-history/
Jacques Derrida 2004 DeathsJacques Derrida 2004 Deaths
Jacques DerridaAge: 74 French philosopher who originated deconstruction, a controversial and widely influential movement that shaped literary theory and a variety of other disciplines, particularly in the 1970s and 1980s.
https://www.infoplease.com/biographies/deaths/2004/jacques-derrida-2004-deaths
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CA2093424A1 - Methods for treating cotton-containing fabrics with cellulase - Google Patents
Disclosed are improved methods for treating cotton-containing fabrics as well as the fabrics produced from these methods. In particular, the disclosed methods are directed to contacting cotton-containing fabrics with a cellulase solution containing a fungal cellulase composition which is substantially,free of all CBH I type cellulase components. Cotton-containing fabrics so treated possess decreased strength loss as compared to fabrics treated with a cellulase solution containing a complete cellulase composition.
https://patents.google.com/patent/CA2093424A1/en
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What is Envoy - envoy tag-v1.10.0 documentationWhat is Envoy - envoy tag-v1.10.0 documentation
Modern C++11 code base: Envoy is written in C++11. Native code was chosen because we believe that an architectural component such as Envoy should get out of the way as much as possible. Modern application developers already deal with tail latencies that are difficult to reason about due to deployments in shared cloud environments and the use of very productive but not particularly well performing languages such as PHP, Python, Ruby, Scala, etc. Native code provides generally excellent latency properties that dont add additional confusion to an already confusing situation. Unlike other native code proxy solutions written in C, C++11 provides both excellent developer productivity and performance.. L3/L4 filter architecture: At its core, Envoy is an L3/L4 network proxy. A pluggable filter chain mechanism allows filters to be written to perform different TCP proxy tasks and inserted into the main server. Filters have already been written to support various tasks such as raw TCP proxy, HTTP proxy, ...
https://www.envoyproxy.io/docs/envoy/v1.10.0/intro/what_is_envoy
Universidade do Minho: Strategies towards reduction of cellulases consumption: debottlenecking the economics of...Universidade do Minho: Strategies towards reduction of cellulases consumption: debottlenecking the economics of...
Lignocellulosic residues have been receiving growing interest as a promising source of polysaccharides, which can be converted into a variety of compounds, ranging from biofuels to bioplastics. Most of these can replace equivalent products traditionally originated from petroleum, hence representing an important environmental advantage. Lignocellulosic materials are theoretically unlimited, cheaper and may not compete with food crops. However, the conversion of these materials to simpler sugars usually requires cellulolytic enzymes. Being still associated with a high cost of production, cellulases are commonly considered as one of the main obstacles in the economic valorization of lignocellulosics. This work provides a brief overview of some of the most studied strategies that can allow an important reduction of cellulases consumption, hence improving the economy of lignocellulosics conversion. Cellulases recycling is initially discussed regarding the main processes to recover active enzymes and ...
https://repositorium.sdum.uminho.pt/handle/1822/72488
Press releases for 4/19/2012
How do I convert a .avi movie onto a disc that will play on a DVD player?Ive shot the movie myself using a camera, it is a two hour long .avi file. All I want is to be able to shove it in the DVD player (in the living room) and for it to automatically play. To play AVI files on DVD player, you need a AVI to DVD Converter or a AVI to DVD burner to help you convert AVI to DVD, burn AVI to DVD. iOrgsoft AVI to DVD Converter is just the ideal AVI to DVD Burner to fast burn AVI to DVD.... ...
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EPO - T 0292/05 () of 25.6.2009EPO - T 0292/05 () of 25.6.2009
Summary of Facts and Submissions. I. The appeal lies from a decision of the Opposition Division revoking European patent 0 699 248, granted on European application No. 95 913 647.4.. II. The application as filed comprised 8 claims, reading, respectively, as follows:. 1. In a method for enhancing the feel and/or appearance and/or for providing color enhancement to a non-cotton containing cellulosic fabrics during manufacture of the fabric by treatment of the fabric with a composition comprising a naturally complete fungal cellulase composition which comprises exo-cellobiohydrolase type component(s) and endoglucanase type component(s) wherein the improvement comprises modifying the naturally complete fungal cellulase composition to comprise at least 10 weight percent of endoglucanase type components based on the total weight of protein in the fungal cellulase composition and be free of all CBH I type cellulase components.. 2. The method according to Claim 1 wherein said fungal cellulase ...
http://www.epo.org/law-practice/case-law-appeals/recent/t050292eu1.html
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decon.] | DC Spensley Studiosdecon.] | DC Spensley Studios
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http://www.dcspensley.com/art/?portfolio=decon
Preschool STEM Play: Computer Deconstruction - Left Brain Craft BrainPreschool STEM Play: Computer Deconstruction - Left Brain Craft Brain
Computer deconstruction is perfect preschool STEM play because tinkering with electronic parts helps kids develop creativity and problem solving skills.
https://leftbraincraftbrain.com/preschool-stem-play-computer-deconstruction/
Catherine Malabou: Does deconstruction have a future? - Frontpage - e-flux conversationsCatherine Malabou: Does deconstruction have a future? - Frontpage - e-flux conversations
[image] In the September issue of the Brooklyn Rail, philosopher Catherine Malabou asks how can Derrida's deconstruction can be renewed today. In her effort to formulate an answer, she suggests that Derrida-along with h…
https://conversations.e-flux.com/t/catherine-malabou-does-deconstruction-have-a-future/4485
Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in...
Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression
http://www.biomedsearch.com/nih/Analysis-conserved-cellulase-transcriptional-regulator/23766336.html
Envoy | diplomat | BritannicaEnvoy | diplomat | Britannica
Other articles where Envoy is discussed: diplomacy: Nature and purpose: …negotiation, primarily conducted by accredited envoys (a term derived from the French envoyé, meaning
https://www.britannica.com/topic/envoy
SOLVED: Ticking noise coming from my 2004 envoy - FixyaSOLVED: Ticking noise coming from my 2004 envoy - Fixya
ticking noise coming from my 2004 envoy about 2 weeks ago i noticed a ticking noise coming from my 2004 gmc envoy. - GMC 2004 Envoy question
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THIS IS A VERY SHORT STORY ABOUT SEAN SPICERS THUMB-SUCKING HABIT 8.24.17 - The DeconstructTHIS IS A VERY SHORT STORY ABOUT SEAN SPICERS THUMB-SUCKING HABIT 8.24.17 - The Deconstruct
Sean Spicer is an adult thumbsucker. Below is part 1 of a many-part series documenting his trials with THE HABIT. The Habit, PART I It started in the womb. Positioned vertically with his head north, it almost looked like he was mocking you in ultrasounds. Sean Spicer was born with his right thumb in his…
https://thedeconstruct.com/2017/08/25/this-is-a-very-short-story-about-sean-spicers-thumb-sucking-habit-8-24-17/
Cluster discovery service - envoy tag-v1.7.0 documentationCluster discovery service - envoy tag-v1.7.0 documentation
The cluster discovery service (CDS) is an optional API that Envoy will call to dynamically fetch cluster manager members. Envoy will reconcile the API response and add, modify, or remove known clusters depending on what is required.. ...
https://www.envoyproxy.io/docs/envoy/v1.7.0/configuration/cluster_manager/cds
Envoy Hospice - Serving TexasEnvoy Hospice - Serving Texas
Envoy provides highly trained professionals and volunteers to assess your patients needs and those of their family.. Envoy provides and pays for these services:. ...
https://www.envoyhospice.com/dierksen_services.php
Royal Envoy 2 - DownloadRoyal Envoy 2 - Download
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Cómo activar o desactivar el modo avión en el celular | Xiaomi Redmi 8 | Soporte Movistar ArgentinaCómo activar o desactivar el modo avión en el celular | Xiaomi Redmi 8 | Soporte Movistar Argentina
Esta guía te muestra cómo activar o desactivar el modo avión en el Redmi 8. Al activar el modo avión se interrumpen todas las funciones de red del...
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Cómo activar o desactivar el modo avión en el celular | Huawei Y5 II | Soporte Movistar ArgentinaCómo activar o desactivar el modo avión en el celular | Huawei Y5 II | Soporte Movistar Argentina
Esta guía te muestra cómo activar o desactivar el modo avión en el Y5 II. Al activar el modo avión se interrumpen todas las funciones de red del...
https://soporteequipos.movistar.com.ar/huawei/y5-ii-android-5-1/como-activar-o-desactivar-el-modo-avion-en-el-celular/
Hyper production of carboxy methyl cellulase by Thermomyces dupontii utilizing physical and chemical mutagenesis |...Hyper production of carboxy methyl cellulase by Thermomyces dupontii utilizing physical and chemical mutagenesis |...
Utilization of cellulases as substitute of chemical process gained huge momentum in the field of biotechnology Now there is dire need to find out un explore reveres of fungi possessing greater potential for efficient cellulase production. This boosted isolation of novel thermo tolerant fungal strains capable of producing the targeted product. In this investigation 70 thermophilic cellulolytic fungal strains were isolated. All the strains were screened via submerged fermentation. The strain showing highest CMCase activity was identified by conventional method i.e. based on morphology and microscopic features and confirmed by 18S rDNA gene sequencing, using specific ITS primers. The modified CTAB method was used for rapid extraction of DNA from thermo tolerant strain. The selected strain subsequently subjected to sequencing and phylogenetic analysis. The result indicates the selected strain was found to be T. dupontii. For strain improvement the T. dupontii was subjected to random mutagenesis by ...
http://rmiq.org/ojs311/index.php/rmiq/article/view/823
Patent US6063611 - Alkaline cellulase and method of producing same - Google PatentsPatent US6063611 - Alkaline cellulase and method of producing same - Google Patents
The present invention provides a novel cellulase composition obtainable from Bacillus sp. CBS 669.93. A preferred cellulase has a calculated molecular weight of approximately 63 kD, a calculated isoelectric point of about 5 and a pH optimum on CMC of about 6 at 40 C. and 60 C.
http://www.google.com.au/patents/US6063611
Omega Pharma Cellulase Anticellulite Mousse 150ml. - FARMACIA INTERNACIONALOmega Pharma Cellulase Anticellulite Mousse 150ml. - FARMACIA INTERNACIONAL
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Commercial Aspects of Biomass Deconstruction with Ionic Liquids | Springer for Research & DevelopmentCommercial Aspects of Biomass Deconstruction with Ionic Liquids | Springer for Research & Development
The adaption of any new process from academic research to industrial-scale requires a robust economic profile demonstrating it can compete with peer technologies. IonoSolv process is a recently...
https://rd.springer.com/chapter/10.1007%2F978-3-030-35245-5_5
Creative Deconstruction: 2008Creative Deconstruction: 2008
Hydrogenation is the process of heating an oil and passing hydrogen bubbles through it. The fatty acids in the oil then acquire some of the hydrogen, which makes it more dense. If you fully hydrogenate, you create a solid (a fat) out of the oil. But if you stop part way, you get a semi-solid partially hydrogenated oil that has a consistency like butter, only its a lot cheaper. Because of that consistency, and because it is cheap, it is a big favorite as a butter-substitute among food? producers. It gives their products a richer flavor and texture, but doesnt cost near as much as it would to add butter. Note: Until the 1970s, food producers used coconut oil to get that buttery flavor and texture. The American obesity epidemic began when it was replaced with partially hydrogenated vegetable oil -- most often soybean oil. ...
http://goliathflores.blogspot.com/2008/
Creative Deconstruction: May 2008Creative Deconstruction: May 2008
Hydrogenation is the process of heating an oil and passing hydrogen bubbles through it. The fatty acids in the oil then acquire some of the hydrogen, which makes it more dense. If you fully hydrogenate, you create a solid (a fat) out of the oil. But if you stop part way, you get a semi-solid partially hydrogenated oil that has a consistency like butter, only its a lot cheaper. Because of that consistency, and because it is cheap, it is a big favorite as a butter-substitute among food? producers. It gives their products a richer flavor and texture, but doesnt cost near as much as it would to add butter. Note: Until the 1970s, food producers used coconut oil to get that buttery flavor and texture. The American obesity epidemic began when it was replaced with partially hydrogenated vegetable oil -- most often soybean oil. ...
http://goliathflores.blogspot.com/2008/05/
Appetite for Deconstruction | San Diego ReaderAppetite for Deconstruction | San Diego Reader
It appears nobody pursued a link-revealing clue in the lyrics: Im looking for something to take/ Im looking for a missing hot snake…. On the publicity one-sheet explaining the albums tracks, singer-guitarist Dimitri Coats revealed all: Its an abstract love song with some venting about our former drummer who was also in Hot Snakes. He was the cheapest guy I ever met. You could buy him eight beers in a row and he wouldnt buy you one. He had real problems spending money and ultimately with being on a major label.. The Hot Snakes were a cool band from San Diego so whenever we played there it was sort of tense, like hanging out with your wifes ex-boyfriend.… Its fitting that the main riff came out of one of the first jams we had with [current drummer] Pete.. The other drummer was Philadelphias Jason Kourkounis, best known in SD lately as J. Sinclair K. of John Reiss new band the Night Marchers. Kourkounis, a founding member of Hot Snakes, played in the band from 1999 to 2003. He ...
https://www.sandiegoreader.com/news/2008/apr/09/blurt3/
JustOneMinute: Zimmerman Case - Eyewitness Contamination and Witness Deconstruction
Jeralyn Merritt tracks the evolution of a witnesss story from her first 911 call on through various media appearances. This is the mystery witness on Anderson Cooper, who saw little and heard less, but has been getting more emphatic over...
https://justoneminute.typepad.com/main/2012/05/zimmerman-case-eyewitness-contamination-and-witness-deconstruction.html?cid=6a00d83451b2aa69e201676691edcc970b
JustOneMinute: Zimmerman Case - Eyewitness Contamination and Witness Deconstruction
Jeralyn Merritt tracks the evolution of a witnesss story from her first 911 call on through various media appearances. This is the mystery witness on Anderson Cooper, who saw little and heard less, but has been getting more emphatic over...
https://justoneminute.typepad.com/main/2012/05/zimmerman-case-eyewitness-contamination-and-witness-deconstruction.html?cid=6a00d83451b2aa69e2016766917d51970b
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as did, of course, Baldwin himself). Rufus begins to treat Leona as cruelly as he did Eric, and begins to beat her because of the psychological torment the he believes she is trying to inflict upon him. (A history of her mental illness is hinted at without much detail.) Enter Vivaldo, a white, aspiring novelist, the linchpin of all the central characters, the center of the network. He visits the apartment of the lovers and finds Rufus sitting on the bed, insane, brandishing a knife. Leona, beaten back and blue, is naked in the bathroom, out of her mind. Vivaldo provides sanctuary. Rufus shortly goes AWOL, dropping out of sight for more than six weeks. Things end disastrously for Rufus and Leona. Vivaldo enters into a relationship with Ida, Rufus younger sister, which seems to share many of the negative qualities of the other couple. Richard and Cass are a slightly older white couple, with two small boys, who have been friends of the group for a long time. (Richard was Vivaldos English teacher ...
http://postmoderndeconstructionmadhouse.blogspot.com/2013/10/another-country-by-james-baldwin.html
Deconstruction of Construction - Coronavirus and Construction UPDATEDeconstruction of Construction - Coronavirus and Construction UPDATE
If you are a property developer or you run a construction company, you may face legal challenges with JCT contracts during the coronavirus pandemic.
https://naylorllp.co.uk/2020/03/25/coronavirus-construction/
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Dalai Lama envoys meet with China - TelegraphDalai Lama envoys meet with China - Telegraph
Special envoys for The Dalai Lama, the exiled Tibetan spiritual leader, have arrived in Beijing for a ninth round of talks with the Chinese authorities over the political status of the Himalayan region.
http://www.telegraph.co.uk/news/worldnews/asia/china/7078572/Dalai-Lama-envoys-meet-with-China.html
Symmetry not guaranteed on border policy: envoy | CTV NewsSymmetry not guaranteed on border policy: envoy | CTV News
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Exogenous cellulase enhances the utilization of ammoniated wheat straw by B. M. Spratling, R. D. Wiedmeier et al.Exogenous cellulase enhances the utilization of ammoniated wheat straw by B. M. Spratling, R. D. Wiedmeier et al.
Spratling, B.M., R.D. Wiedmeier, D.R. ZoBell, C.A. Fitzgerald, and B.A. Kent. 2001. Exogenous cellulase enhances the utilization of ammoniated wheat straw in pregnant and lactating beef cows. Proceedings, Western Section, American Society of Animal Science. Vol. 52:349-352.. ...
https://digitalcommons.usu.edu/advs_facpub/629/
CellulaseCellulase
... cellulase A, cellulosin AP, alkali cellulase, cellulase A 3, 9.5 cellulase, and pancellase SS. Enzymes that cleave lignin have ... Cellulose 1,4-beta-cellobiosidase, an efficient cellulase Cellulase unit, a unit for quantifying cellulase activity PDB: 1NLR​ ... Cellulase action is considered to be synergistic as all three classes of cellulase can yield much more sugar than the addition ... Progressive cellulase will continue to interact with a single polysaccharide strand, nonprogressive cellulase will interact ...
https://en.wikipedia.org/wiki/Cellulase
Cellulase unitCellulase unit
A cellulase unit (CU) is a unit of measurement for the enzyme cellulase. One cellulase unit is that activity that will produce ... Cellulases enzymatically split cellulose into a variety of hexoses, this is called hydrolysis. Eagle Pack Archived February 21 ...
https://en.wikipedia.org/wiki/Cellulase_unit
AlkaliphileAlkaliphile
... cellulases; lipases; xylanases; pectinases; chitinases and their metabolites, including: 2-phenylamine; carotenoids; ...
https://en.wikipedia.org/wiki/Alkaliphile
CelluloseCellulose
A cellulase may function to cleave the primer from the mature chain. Cellulose is also synthesised by tunicate animals, ... The enzymes used to cleave the glycosidic linkage in cellulose are glycoside hydrolases including endo-acting cellulases and ... Tokuda, G; Watanabe, H (22 June 2007). "Hidden cellulases in termites: revision of an old hypothesis". Biology Letters. 3 (3): ... "Fungal Cellulases". Chemical Reviews. 115 (3): 1308-1448. doi:10.1021/cr500351c. PMID 25629559. Mettler, Matthew S.; Vlachos, ...
https://en.wikipedia.org/wiki/Cellulose
Fungal extracellular enzyme activityFungal extracellular enzyme activity
One soft-rot Ascomycete, Trichoderma reesei, is used extensively in industrial applications as a source for cellulases and ... Wilson, David B (2009). "Cellulases and biofuels". Current Opinion in Biotechnology. 20 (3): 295-299. doi:10.1016/j.copbio. ... Extracellular enzymes target macromolecules such as carbohydrates (cellulases), lignin (oxidases), organic phosphates ( ... production of extracellular enzymes and characterization of the major cellulases". Microbiology. 152 (12): 3613-3622. doi: ...
https://en.wikipedia.org/wiki/Fungal_extracellular_enzyme_activity
CellobioseCellobiose
Wilson, David B. (2009). "Cellulases and biofuels". Current Opinion in Biotechnology. 20 (3): 295-299. doi:10.1016/j.copbio. ...
https://en.wikipedia.org/wiki/Cellobiose
Harry J. GilbertHarry J. Gilbert
Gilbert, H. J.; Hazlewood, G. P. (1993). "Bacterial cellulases and xylanases". Journal of General Microbiology. 139 (2): 187- ...
https://en.wikipedia.org/wiki/Harry_J._Gilbert
LichenaseLichenase
Barras DR, Moore AE, Stone BA (1969). "Enzyme-Substrate Relationships Among β-Glucan Hydrolases". Cellulases and Their ...
https://en.wikipedia.org/wiki/Lichenase
TheodoxusTheodoxus
Green algae[clarification needed] are not consumed; Theodoxus has no cellulases. These snails lay egg capsules containing 30-70 ...
https://en.wikipedia.org/wiki/Theodoxus
Enzyme washingEnzyme washing
In the enzyme wash process, cellulases act on exposed cellulose on the cotton fibers, freeing indigo dye from the fabric. The ... "Stone" Washing Jeans: Cellulases". IUPUI Department of Biology. "Enzymes make your jeans less thirsty". Novozymes. (Textile ... Enzyme washing or "bio stoning" is a textile manufacturing technique in which cellulase enzymes are applied to denim. As with ... ENZYME WASHING INFORMATION Bhat, M.K. (2000). "Cellulases and related enzymes in biotechnology". Biotechnology Advances. 18 (5 ...
https://en.wikipedia.org/wiki/Enzyme_washing
Penicillium verruculosumPenicillium verruculosum
Szakács, György; Réczey, Kati; Hernádi, Pál; Dobozi, Mária (1981). "Penicillium verruculosum WA 30 a new source of cellulase". ... "Cellulases of Penicillium verruculosum". Biotechnology Journal. 5 (8): 871-80. doi:10.1002/biot.201000050. PMID 20540109. Shah ... "Cellulases of Penicillium verruculosum". Biotechnology Journal. 5 (8): 871. doi:10.1002/biot.201000050. PMID 20540109. Wigney, ... "Enzymatic hydrolysis of pre-treated lignocellulose with Penicillium verruculosum cellulases". Journal of Molecular Catalysis B ...
https://en.wikipedia.org/wiki/Penicillium_verruculosum
Thermomyces lanuginosusThermomyces lanuginosus
T. lanuginosis is unable to utilize cellulose because it does not produce a cellulase, but it is well adapted to using other ... Kuhad, Ramesh Chander; Gupta, Rishi; Singh, Ajay (2011-09-07). "Microbial Cellulases and Their Industrial Applications". Enzyme ...
https://en.wikipedia.org/wiki/Thermomyces_lanuginosus
GeyserGeyser
Industrial Uses of Thermophilic Cellulase University of Delaware, Retrieved on 2008-03-29 Archived October 10, 2007, at the ... Bhat, M.K. (2000). "Cellulases and related enzymes in biotechnology". Biotechnology Advances. 18 (5): 355-383. doi:10.1016/ ... An Extremely Thermostable Cellulase from the Thermophilic Eubacterium Rhodothermus marinus. Applied and Environmental ... "Treatment of recycled fiber with Trichoderma cellulases". Industrial Crops and Products. 20: 11-21. doi:10.1016/j.indcrop. ...
https://en.wikipedia.org/wiki/Geyser
ExoenzymeExoenzyme
Cellulases and hemicellulases (including xylanases) are also used in the paper and pulp industry to de-ink recycled fibers, ... Cellulases and hemicellulases are used in these industrial applications due to their ability to hydrolyze the cellulose and ... Cellulases, hemicellulases and pectinases are different exoenzymes that are involved in a wide variety of biotechnological and ... In addition to the role they play in food production, cellulases are used in the textile industry to remove excess dye from ...
https://en.wikipedia.org/wiki/Exoenzyme
Chaetomium cellulolyticumChaetomium cellulolyticum
It is associated with the production of cellulase. Leisola, Matti; Ulmer, Duane; Pitkänen, Kari; Fiechter, Armin (1985-10-01 ... "Induction of Cellulases in chaetomium cellulolyticumby cellobiose". Biotechnology and Bioengineering. 27 (9): 1389-91. doi: ...
https://en.wikipedia.org/wiki/Chaetomium_cellulolyticum
Fibrobacter succinogenesFibrobacter succinogenes
Thirty-one genes are identified as cellulases. The genome also encodes for a number of proteins capable of breaking down sugars ...
https://en.wikipedia.org/wiki/Fibrobacter_succinogenes
Trichoderma virideTrichoderma viride
The cellulase produced by the fungus partially degrade the cotton material in places, making it soft and causing the jeans to ... The mycelium of T. viride can produce a variety of enzymes, including cellulases and chitinases which can degrade cellulose and ... Treating Denim Fabrics with Trichoderma Reesei Cellulases. Textile Research Journal, 70(11), 969-973. https://doi.org/10.1177/ ...
https://en.wikipedia.org/wiki/Trichoderma_viride
Streptomyces viridobrunneusStreptomyces viridobrunneus
... produces cellulase. List of Streptomyces species LPSN bacterio.net Straininfo of Streptomyces ... "Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic biomass as inducer substrate". Applied ... "Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic biomass as inducer substrate". Applied ...
https://en.wikipedia.org/wiki/Streptomyces_viridobrunneus
Cellulosic ethanolCellulosic ethanol
The cellulase family of enzymes have a one to two order smaller magnitude of efficiency. Therefore, it requires 40 to 100 times ... For cellulase produced offsite, enzyme production amounts to 36% of cash cost. For enzyme produced onsite in a separate plant, ... A recent paper estimates the range at 13-36% of cash costs, with a key factor being how the cellulase enzyme is produced. ... Cellulose chains can be broken into glucose molecules by cellulase enzymes. This reaction occurs at body temperature in the ...
https://en.wikipedia.org/wiki/Cellulosic_ethanol
Penicillium occitanisPenicillium occitanis
The mutant Pol6 produces a very high amount of cellulase and pectinase. This mutant might be used for industrial use. Belghith ... Ellouz Chaabouni, S.; Belguith, H.; Hassairi, I.; m'Rad, K.; Ellouz, R. (1995). "Optimization of cellulase production by ... Penicillium occitanis is a species of fungus in the genus Penicillium which produces cellulase and pectinase. ... Jain, S.; Parriche, M.; Durand, H.; Tiraby, G. (1990). "Production of polysaccharidases by a cellulase-pectinase hyperproducing ...
https://en.wikipedia.org/wiki/Penicillium_occitanis
Non-homologous isofunctional enzymesNon-homologous isofunctional enzymes
Another popular example of NISE are the cellulase family of enzymes, particularly Cellulose 1,4-beta-cellobiosidase also ... Sukharnikov, Leonid O.; Cantwell, Brian J.; Podar, Mircea; Zhulin, Igor B. (2011). "Cellulases: Ambiguous nonhomologous enzymes ...
https://en.wikipedia.org/wiki/Non-homologous_isofunctional_enzymes
Glycoside hydrolase family 5Glycoside hydrolase family 5
One of these families is known as the cellulase family A or as the glycosyl hydrolases family 5. One of the conserved regions ... Py B, Bortoli-German I, Haiech J, Chippaux M, Barras F (February 1991). "Cellulase EGZ of Erwinia chrysanthemi: structural ... Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence ... Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon JP (September 1989). "Cellulase families revealed by hydrophobic cluster ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_5
Oesophageal pouchOesophageal pouch
"The Distribution of Cellulases and Related Enzymes in Mollusca". Journal of Molluscan Studies. 1958. doi:10.1093/oxfordjournals ...
https://en.wikipedia.org/wiki/Oesophageal_pouch
Greige goodsGreige goods
"Scouring of Cotton with Cellulases, Pectinases and Proteases" (PDF).{{cite web}}: CS1 maint: url-status (link) Trotman, E. R. ( ...
https://en.wikipedia.org/wiki/Greige_goods
Clostridium cellulovoransClostridium cellulovorans
"Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein ... "Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans". Applied Microbiology and Biotechnology. 71 (5 ...
https://en.wikipedia.org/wiki/Clostridium_cellulovorans
Scouring (textiles)Scouring (textiles)
"Scouring of Cotton with Cellulases, Pectinases and Proteases" (PDF).{{cite web}}: CS1 maint: url-status (link) Montazer, Majid ...
https://en.wikipedia.org/wiki/Scouring_(textiles)
Myceliophthora thermophilaMyceliophthora thermophila
Cellulases are rapidly synthesized by M. thermophila and can be used to degrade cellulose into simple carbohydrates as a food ... Coutts, A.D.; Smith, R.E. (1976). "Factors influencing the Production of Cellulases by Sporotrichum thermophile". Appl. Environ ...
https://en.wikipedia.org/wiki/Myceliophthora_thermophila
TermiteTermite
Li, Z.-Q.; Liu, B.-R.; Zeng, W.-H.; Xiao, W.-L.; Li, Q.-J.; Zhong, J.-H. (2013). "Character of Cellulase Activity in the Guts ... Watanabe, H..; Noda, H.; Tokuda, G.; Lo, N. (1998). "A cellulase gene of termite origin". Nature. 394 (6691): 330-331. Bibcode: ... Most higher termites, especially in the family Termitidae, can produce their own cellulase enzymes, but they rely primarily ... Tokuda, G.; Watanabe, H. (2007). "Hidden cellulases in termites: revision of an old hypothesis". Biology Letters. 3 (3): 336- ...
https://en.wikipedia.org/wiki/Termite
Ulocladium chartarumUlocladium chartarum
Cellulase activities of U. chartarum were found to be influenced by the mode of incubation as well as by the age of the fungal ... Sallam, Lotfy; H.El-Refai, Abdel-Monem; El-Sayed, Samia M. (1988). "Cellulase production by halophytic fungi: II. The role of ... some cultural conditions on cellulase production by Ulocladium chartarum". Zentralblatt für Mikrobiologie. 143 (8). doi:10.1016 ...
https://en.wikipedia.org/wiki/Ulocladium_chartarum
Chaetomium globosumChaetomium globosum
The cellulase activity of C. globosum functions best at temperatures ranging from 25-32 degrees Celsius and is stimulated by ... Cellulase is inhibited by ethyl malonate. Like many fungal species, C. globosum obtains their energy from carbon sources such ... Like most Chaetomium species, C. globosum decomposes plant cells using hyphal cellulase activity. Even though they are known to ...
https://en.wikipedia.org/wiki/Chaetomium_globosum
Cellulase Market To Develop Strongly And Cross USD 3.14 billion By 2031 - PharmiWeb.comCellulase Market To Develop Strongly And Cross USD 3.14 billion By 2031 - PharmiWeb.com
Ltd -:Cellulase Market: Drivers & Restraints The overall market for Cellulase is driven by rising cancer incidences and ... Global Market Challenges Cellulase. 1. Even if research costs are minimal, producing Cellulase is a complex and costly process ... Ltd -:Cellulase Market: Drivers & Restraints The overall market for Cellulase is driven by rising cancer incidences and ... 5. Producers of Cellulase products must compete with other Cellulase producers and the original biological and generic drug ...
https://www.pharmiweb.com/press-release/2022-11-08/cellulase-market-to-develop-strongly-and-cross-usd-314-billion-by-2031
Cellulase enzymes (5gm)(BN/EXP) - SciChemCellulase enzymes (5gm)(BN/EXP) - SciChem
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https://scichem.com/product/cellulase-enzymes-5gmbn-exp/
Expression of a Fungal Cellulase Gene by \beta-glucanase Promoter of S by MEHMET SAİT EKİNCİ"Expression of a Fungal Cellulase Gene by \beta-glucanase Promoter of S" by MEHMET SAİT EKİNCİ
EKİNCİ, MEHMET SAİT (2001) "Expression of a Fungal Cellulase Gene by \beta-glucanase Promoter of Streptococcus bovis," Turkish ... Expression of a Fungal Cellulase Gene by \beta-glucanase Promoter of Streptococcus bovis ...
https://journals.tubitak.gov.tr/biology/vol25/iss1/4/
Production Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of DietProduction Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of Diet
Effect of culture incubation period on cellulase production: Production of cellulase on modified CPM was monitored for a period ... Culture conditions for cellulase production: Static cultures were used for studying fungal cellulase production under variable ... Cellulase enzyme production by Penicillium chrysogenum in different pH of modified CPM produced the highest value of cellulase ... Effect of culture incubation period on cellulase production. The highest cellulase activity (5.55 μmole mL 1 min 1) (p,0.05) ...
https://scialert.net/fulltext/?doi=ijds.2019.61.68&org=11#ref
Cellulose hydrolysis by Trichoderma reesei cellulases: Studies on adsorption, sugar production and synergism of...Cellulose hydrolysis by Trichoderma reesei cellulases: Studies on adsorption, sugar production and synergism of...
Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of ,i,Trichoderma reesei,/i, ... Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of ,i,Trichoderma reesei,/i, ... FPLC, cellulose hydrolysis, Trichoderma reesei, cellulase, cellobiohydrolase, endoglucanase, adsorption isotherm, synergism, ... phdthesis{271a2c21-535c-4129-9648-9d89ecf5855e, abstract = {{Three major cellulases, cellobiohydrolase I and II (CBH I and II) ...
https://lup.lub.lu.se/search/publication/29036
Distribution of cellulases in the body of Coptotermes formosanus and the probability that the termite uses glucose as an energy...Distribution of cellulases in the body of Coptotermes formosanus and the probability that the termite uses glucose as an energy...
Distribution of cellulases in the body of Coptotermes formosanus and the probability that the termite uses glucose as an energy ... Thus all the necessary cellulases for hydrolysis of natural cellulose to glucose were present in the region ranging from the ... Keywords: LOWER TERMITE; COPTOTERMES FORMOSANUS; CELLULASE; PYRUVATE DEHYDROGENASE; SALIVARY GLANDS; MIDGUT Conference: 97-05- ... extracts of the digestive system and of the whole body of Coptotermes formosanus to determine where the various cellulases, ...
https://www.irg-wp.com/irgdocs/details.php?29efc563-55d6-4da7-9bc9-45ce7da8ee3a
Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses | BMC Research Notes |...Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses | BMC Research Notes |...
The molecular weight of the purified cellulase was found to be 67 kDa by SDS-PAGE, had an optimal pH and temperature at ... showed high potentiality for maximum cellulase production (0.9 µmol ml−1 min−1) at pH 7.0 after 24 h of ... agar plate were identified as cellulase producing bacteria. Different culture parameters such as pH, temperature, incubation ... 7.0 and 40 °C. According to substrate specificity, the purified cellulase had high specificity on CMC substrate which ...
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3558-4
Regulation of cellulase expression in Trichoderma reesei</em>...Regulation of cellulase expression in Trichoderma reesei</em>...
Ilmen, M., Onnela, M-L., Saloheimo, A., & Penttilä, M. (1992). Regulation of cellulase expression in Trichoderma reesei. Paper ... Regulation of cellulase expression in Trichoderma reesei. / Ilmen, Marja; Onnela, Maija-Leena; Saloheimo, Anu et al. ... title = "Regulation of cellulase expression in Trichoderma reesei",. author = "Marja Ilmen and Maija-Leena Onnela and Anu ... Ilmen, M, Onnela, M-L, Saloheimo, A & Penttilä, M 1992, Regulation of cellulase expression in Trichoderma reesei, Paper ...
https://cris.vtt.fi/en/publications/regulation-of-cellulase-expression-in-trichoderma-reesei
Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea | BMC...Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea | BMC...
By whole genome sequencing, we found 10 cellulase genes from the three isolates. We compared the expression of these cellulase ... microbial cellulases by isolating cellulase-producing microorganisms from the Red Sea environment and characterizing cellulase ... Although cellulases have been roughly classified into three enzyme groups of beta-glucosidase, endo-β-1,4-glucanase and ... However, there is little information regarding cellulase genes in the Red Sea environment. This study aimed to examine whether ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-021-02316-w
Cellulases
... has the Law Prioritize fonder. Nescio cellulases tailor jacentem advertising. prove cellulases, you original adults ... Gavin The cellulases of day under the Eighteenth Amendment left Together a? The cellulases of the industry place was from long ... 15-20 employees to discuss their cellulases. ;. Cellulases. * Robynne Jeisman Maryann Cusimano-Love and Catholic University ... No cellulases or anyone. *Plaza cellulases reason, ca 94534( 707) 864-1040 ausi impulses plunge History is one of the writer ...
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reposiTUm: Regulation of cellulase formation by lactosereposiTUm: Regulation of cellulase formation by lactose
Regulation of cellulase formation by ...
https://repositum.tuwien.at/cris/project/pj02055
In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization | BMC...In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization | BMC...
Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In the present ... From: In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-021-00687-6/figures/4
Investigation of the binding properties of a multi-modular GH45 cellulase using bioinspired model assemblies - Aix-Marseille...
The affinity of the multi-modular GH45 cellulase was characterized using a statistical analysis to determine the role played by ... Depending on the number of CBMs appended to the cellulase catalytic core, binding properties toward cellulose and lignin were ... used lignified model assemblies of plant cell walls as templates to characterize binding properties of multi-modular cellulases ... Investigation of the binding properties of a multi-modular GH45 cellulase using bioinspired model assemblies Fong M 1 Berrin Jg ...
https://hal-amu.archives-ouvertes.fr/hal-01450838
CEL - Cellulase - CellSystems®CEL - Cellulase - CellSystems®
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Cellulase Archives | BAKERpediaCellulase Archives | BAKERpedia
Cellulase. Cellulase What is Cellulase? Cellulase is one type of hydrolytic enzyme which targets cellulose fibers. Cellulose is ... Cellulases are typically used in coffee processing and fruit juice extraction. Although not widely distributed in [...] ...
https://bakerpedia.com/tag/cellulase/
cellulase Archives - Entomology Todaycellulase Archives - Entomology Today
Tag: cellulase. Researchers Discover How Stick Insects Digest Cellulose Plant cell walls are comprised of many complex polymers ... In order to be digested, these polymers must be broken down by enzymes such as cellulase […] ...
https://entomologytoday.org/tag/cellulase/
IMSEAR at SEARO: Cellulase Production by Bacteria: A Review.
The review discusses application of cellulase, classification of cellulase, quantification of cellulase, the types of ... properties of cellulase and cloning and expression of cellulase gene. The biotechnological aspect of cellulase research and ... Cellulase Production by Bacteria: A Review. British Microbiology Research Journal. 2013 July; 3(3): 235-258. ... It can be degraded by cellulase produced by cellulolytic bacteria. This enzyme has various industrial applications and now ...
https://imsear.searo.who.int/handle/123456789/162913?locale=ar
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Cellulase buy online original products. Find best prices for Cellulase ... Cellulase. Cellulase Advanced from Cellulase is the ideal solution to fight the most advanced states of orange peel skin with ... Cellulase. Cellulase Pearls from Cellulase is the suitable solution for women with "orange peel" skin in a light condition for ... Cellulase. Xanthigen by Cellulase from Cellulase acts on the origin of accumulation of fat supporting the metabolism of fats ...
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Role of ionic liquids in the enzyme stabilization: a case study with Trichoderma ressie cellulase - IIUM Repository (IRep)Role of ionic liquids in the enzyme stabilization: a case study with Trichoderma ressie cellulase - IIUM Repository (IRep)
In this study, cellulase was therefore tested in several ILs. This approach opens an insight for further studies to ... Ionic liquid, Lignocellulose, Cellulase, Hydrolysis, Stability · Activity. Subjects:. T Technology , TP Chemical technology , ... This chapter discusses the stabilization of Trichoderma ressie cellulase (Tri-Cel) in ionic liquids (ILs) to enable the in situ ... Role of ionic liquids in the enzyme stabilization: a case study with Trichoderma ressie cellulase ...
http://irep.iium.edu.my/91605/
Neutral Cellulase Enzyme on sales - Quality Neutral Cellulase Enzyme supplierNeutral Cellulase Enzyme on sales - Quality Neutral Cellulase Enzyme supplier
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Conzyme DL Ultra Acid cellulase - Industrial Enzyme - Sunson shopping mallConzyme DL Ultra Acid cellulase - Industrial Enzyme - Sunson shopping mall
Conzyme DL Ultra is a liquid high concentrated acid cellulase preparation. It is specially developed for fabric and garment ... PRODUCT DESCRIPTION Hemi-Cellulase SBE-02X is produced by submerged fermentation of Trichoderma reesei followed by purification ... Conzyme DL Ultra is a liquid high concentrated acid cellulase preparation. It is specially developed for fabric and garment ... Conzyme NL Extra is a high concentrated liquid neutral cellulase preparation. It is specially developed for fabric and garment ...
http://en.sunsonenzymes.com/prod_view.aspx?typeid=87&id=284&fid=t3:87:3
Surfactant-induced unfolding of cellulase: Kinetic studies | CU Experts | CU BoulderSurfactant-induced unfolding of cellulase: Kinetic studies | CU Experts | CU Boulder
Data updated last 11/30/2022 22:15 10:15:01 PM ...
https://experts.colorado.edu/display/pubid_17611
A truncated form of the Carbon catabolite repressor 1 increases cellulase production in Trichoderma reesei | Biotechnology for...A truncated form of the Carbon catabolite repressor 1 increases cellulase production in Trichoderma reesei | Biotechnology for...
... what exactly enhances cellulase production in Rut-C30. The investigation of the expression of two genes encoding cellulases ( ... in particular cellulases. Due to three rounds of undirected mutagenesis its genetic background differs from the wild-type QM6a ... Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains ... Determination of cellulase activity. Cellulase activity in the culture supernatants was determined using AZCL HE-Cellulose ( ...
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-014-0129-3
Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis<...Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis<...
Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis. In: PLoS ONE. 2015 ; Vol. ... Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis. PLoS ONE. 2015 May 29;10(5 ... Dive into the research topics of Significance of relative position of cellulases in designer cellulosomes for optimized ... Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis. / Stern, Johanna; Kahn, ...
https://cris.tau.ac.il/en/publications/significance-of-relative-position-of-cellulases-in-designer-cellu
Biotechnology Articles, Find Biotechnology Articles on Sooper ArticlesBiotechnology Articles, Find Biotechnology Articles on Sooper Articles
Application of Cellulase in Food Industry. As people pay more and more attention to food safety, nutrition, health and ...
https://www.sooperarticles.com/technology-articles/biotechnology-articles/
Microorganisms | Free Full-Text | Parallel Evolution of Enhanced Biofilm Formation and Phage-Resistance in Pseudomonas...Microorganisms | Free Full-Text | Parallel Evolution of Enhanced Biofilm Formation and Phage-Resistance in Pseudomonas...
In Cellulases; Elsevier BV: Amsterdam, The Netherlands, 1999; Volume 310, pp. 91-109. [Google Scholar] ...
https://www.mdpi.com/2076-2607/9/3/569
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Polypeptides having cellulolytic enhancing activity and polynucleotides encoding the same (Patent) | DOE PatentsPolypeptides having cellulolytic enhancing activity and polynucleotides encoding the same (Patent) | DOE Patents
C12N9/2437 - {Cellulases C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES C07K14/37 - from fungi. C - CHEMISTRY C12 - ...
https://www.osti.gov/doepatents/biblio/1108724
Cellulosic ethanol - WikipediaCellulosic ethanol - Wikipedia
The cellulase family of enzymes have a one to two order smaller magnitude of efficiency. Therefore, it requires 40 to 100 times ... A recent paper[73] estimates the range at 13-36% of cash costs, with a key factor being how the cellulase enzyme is produced. ... For cellulase produced offsite, enzyme production amounts to 36% of cash cost. For enzyme produced onsite in a separate plant, ... Cellulose chains can be broken into glucose molecules by cellulase enzymes. This reaction occurs at body temperature in the ...
https://en.wikipedia.org/wiki/Cellulosic_ethanol
TrichodermaEnzymesProteaseHydrolysisXylanaseFermentationMicrobial cellulasesApplication of cellulaseMaximum cellulaseCellulosomesGlucoseCellulolyticGeneHydrolytic enzymeBacteriaBiomassLignocellulosicAdsorptionProductionSubstrateBacillusVitroCarboxymethylAgriculturalGenesAgarCatalyticHttpsCellulolysisActivityStrainComplexIsolatesAgricultureSugarStudiesStudy
Trichoderma5
  • Several cellulase-producing microorganisms with high cellulolytic activity have been isolated from the fungal genera Aspergillus and Trichoderma [ 6 ]. (biomedcentral.com)
  • This chapter discusses the stabilization of Trichoderma ressie cellulase (Tri-Cel) in ionic liquids (ILs) to enable the in situ hydrolysis of cellulosic and lignocellulosic substances. (iium.edu.my)
  • PRODUCT DESCRIPTION Hemi-Cellulase SBE-02X is produced by submerged fermentation of Trichoderma reesei followed by purification, formulation and drying. (sunsonenzymes.com)
  • Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulases. (biomedcentral.com)
  • 23. Domingues FC, Queiroz JA, Cabral JM, Fonseca LP. The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30. (bvsalud.org)
Enzymes13
  • Cellulases enzymes are widely be interested due to its capability to degrade lignocellulosic materials. (scialert.net)
  • Cellulase is a family of enzymes capable of hydrolyzing β-1,4-glycosidic bonds of intact cellulose and other related cello-oligosaccharide derivatives. (scialert.net)
  • Fungal cellulase is the main source of commercially produced enzymes for diet additives while, few bacteria and actinomycetes have also been investigated for the production of cellulolytic enzymes 5 - 9 . (scialert.net)
  • Mostly the cellulase enzymes production was reported from bacteria and fungi 1 , 2 . (scialert.net)
  • The cellulases can effectively hydrolyze cellulose into glucose unit via the synergistic actions of the enzymes, known as endo-β-1,4 glucanase, cellobiohydrolase and β-D-glucosidase [ 7 ]. (biomedcentral.com)
  • Although cellulases have been roughly classified into three enzyme groups of beta-glucosidase, endo-β-1,4-glucanase and exoglucanase, these findings suggest the importance to consider microbial cellulolysis as a more complex reaction with various kinds of cellulase enzymes. (biomedcentral.com)
  • Cellulases are a group of three types of enzymes with different activities. (biomedcentral.com)
  • The information is also given on the processing of agricultural biomass to produce industrially important enzymes, e.g., ligninases or cellulases. (cyberleninka.org)
  • The most promising technology for the conversion of the lignocellulosic biomass to fuel ethanol is based on the enzymatic breakdown of cellulose using cellulase enzymes [2,3,7]. (cyberleninka.org)
  • Yeast have cell walls made of cellulose, which cannot be digested or broken down by human digestive enzymes as we do not produce cellulase, the enzyme needed to break down cellulose. (minavit.com)
  • Candifense contains a variety of of potent cellulase and fiber digesting enzymes specifically chosen to help support a healthy balance of probiotic bacteria in the gut. (minavit.com)
  • Other enzymes have also been reported as sensitizers in commercial bakery operations and include cellulase, f3-xylosidase, and glucoamylase. (cdc.gov)
  • Using an IBM Aptiva S-series computer and data from the Protein Database of the US Department of Energy's Brookhaven National Laboratory, she and a colleague examined the structure-function relationships of cellulases, enzymes that break down cellulose. (nkeconwatch.com)
Protease1
  • Thermostable alpha-amylase, cellulase and neutral protease are also used, the latter for improving the flavour. (chinafoodingredients.com)
Hydrolysis7
  • Thus all the necessary cellulases for hydrolysis of natural cellulose to glucose were present in the region ranging from the salivary glands to the midgut in significant amounts. (irg-wp.com)
  • The biodegradation of β-1,4-glycosidic bonds in cellulose biomass is carried out by free cellulases or a multienzyme complex referred to as the cellulosome, which can catalyze the hydrolysis of cellulose into sugars. (biomedcentral.com)
  • Besides all those applications, the production of fuel ethanol from lignocellulosic biomass through cellulase hydrolysis is a promising tool of the modern world. (cyberleninka.org)
  • The hydrolysis activity of the cellulase mutant (BglC-M) was approximately 2.5-fold higher than the control (BglC-W) over a wide range of pH and temperature conditions. (wu.ac.th)
  • Finally, the biomass hydrolysis ability of cellulase was investigated on lignocellulosic wastes such as pineapple peel, corncob, and durian peel. (wu.ac.th)
  • The hydrolysis of its main component cellulose to glucose necessitates a cellulase system consisting of cellobiohydrolase (CBH, E.C. (cyberleninka.org)
  • for direct physical contact between cellulase and cellulose and, therefore, influences cellulase adsorption as well as the rate and extent of cellulose hydrolysis [21,25]. (cyberleninka.org)
Xylanase1
  • Kim, SW , Moon, KH & Tae, BS 1996, ' Simple and rapid method for the isolation of Aspergillus niger mutants with enhanced cellulase and xylanase activity ', Biotechnology Techniques , vol. 10, no. 10, pp. 735-736. (elsevier.com)
Fermentation5
  • The current study was concerning on investigating different factors of cellulase production from fungal sources by using agricultural wastes and studying its impacts on ruminal digestion and fermentation. (scialert.net)
  • Therefore, using high yielding strains, optimal fermentation conditions and cheap production substrate is the way for maximum and economical cellulase production, especially with the increasing interest with utilizing cellulase in ruminant's diets. (scialert.net)
  • So, the current study was carried out to investigate utilization of some agricultural by-products as substrate to produce fungal cellulase under the optimum fermentation conditions and the impacts of cellulase on ruminal in vitro degradability and fermentation. (scialert.net)
  • It describes the current knowledge of cellulase production by submerged fermentation and solid state fermentation, properties of cellulase and cloning and expression of cellulase gene. (who.int)
  • Objective: The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. (herts.ac.uk)
Microbial cellulases2
  • However, microbial cellulases varied according to the microbial strain, cultivation conditions (such as pH, incubation period and inoculums size) in addition to growth medium composition particularly carbon and nitrogen sources 3 , 4 . (scialert.net)
  • This study aimed to examine whether the Red Sea can be a resource for the bioprospecting of microbial cellulases by isolating cellulase-producing microorganisms from the Red Sea environment and characterizing cellulase genes. (biomedcentral.com)
Application of cellulase1
  • The review discusses application of cellulase, classification of cellulase, quantification of cellulase, the types of cellulolytic bacteria and their screening. (who.int)
Maximum cellulase3
  • showed high potentiality for maximum cellulase production (0.9 µmol ml −1 min −1 ) at pH 7.0 after 24 h of incubation at 40 °C in a medium containing 1.0% CMC. (biomedcentral.com)
  • Afterwards, the effects of swollenin on cellulose particle size, maximum cellulase adsorption and cellulose crystallinity were quantified. (cyberleninka.org)
  • The pretreatment with swollenin resulted in a significant decrease in particle size of the cellulosic substrates as well as in their crystallinity, thereby substantially increasing maximum cellulase adsorption onto these substrates. (cyberleninka.org)
Cellulosomes1
  • She studied plant cell wall degradation, cellulases and cellulosomes, and biocompound production by various microorganisms, as well as on tolerance mechanisms of microbes toward chemicals. (caltech.edu)
Glucose3
  • Moreover, cellulase enzyme can be an effective additive with agricultural by-product to produce simple glucose units which play a direct role in animals feeding by improved digestion in ruminants 14 . (scialert.net)
  • We assayed extracts of the digestive system and of the whole body of Coptotermes formosanus to determine where the various cellulases, glucose, and related substances were concentrated and to detect pyruvate dehydrogenase activity in the hindgut-removed body in order to verify its full cellulolytic system. (irg-wp.com)
  • Cellulose a glucose-based biopolymer, and structural components of paper material can be degraded into glucose by cellulase a hydrolytic enzyme system. (ijpsr.com)
Cellulolytic1
  • It can be degraded by cellulase produced by cellulolytic bacteria. (who.int)
Gene2
  • As represented by B. subtilis , a lot of studies regarding cellulase gene sequences, enzymatic activities, optimal condition for cellulolysis were published from Bacillus species. (biomedcentral.com)
  • The cellulase gene ( BglC-M ) from the mutant was expressed in Escherichia coli BL21(DE3) under the T7 promoter. (wu.ac.th)
Hydrolytic enzyme1
  • Cellulase is one type of hydrolytic enzyme which targets cellulose fibers. (bakerpedia.com)
Bacteria7
  • This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. (biomedcentral.com)
  • Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC) agar plate were identified as cellulase producing bacteria. (biomedcentral.com)
  • Cellulose is mainly degraded by cellulase enzyme which is commonly produced by bacteria and fungi [ 6 ]. (biomedcentral.com)
  • The growth rate of bacteria is faster than fungi and has been widely used in cellulase production under different culture conditions [ 13 ]. (biomedcentral.com)
  • Therefore, this study was conducted to screening, optimization, purification and characterization of cellulase from cellulase producing bacteria present in molasses. (biomedcentral.com)
  • Our study reports the isolation and characterization of cellulase-producing bacteria from the Red Sea. (biomedcentral.com)
  • IMSEAR at SEARO: Cellulase Production by Bacteria: A Review. (who.int)
Biomass2
  • There are few studies on cellulase production from raw biomass such as rice straw 4 . (scialert.net)
  • Cellulases provide a key opportunity for achieving tremendous benefits of biomass utilization. (cyberleninka.org)
Lignocellulosic1
  • The study tested the cellulase enzyme production ability of fungal strains against different lignocellulosic. (scialert.net)
Adsorption2
  • According to the adsorption isotherm results, EG II has the most binding sites on the substrate, while CBH I has the strongest capability to displace other cellulases. (lu.se)
  • The adsorption parameters of the recombinant swollenin onto cellulose were quantified for the first time and were comparable to those of individual cellulases from T. reesei. (cyberleninka.org)
Production6
  • Simultaneously the effect of different fungal strains to choose according to the superiority of cellulase production, then environmental factors were studied such as carbon source concentrations, inoculum size, incubation period, initial pH and nitrogen source. (scialert.net)
  • Pea pods showed best carbon source with 17.5% concentration for cellulase production. (scialert.net)
  • About 4% inoculum size, 2 days of incubation and pH5 were recorded the highest value for cellulase production. (scialert.net)
  • Many research being interested with utilizing agricultural as substrate for cellulases production 2 - 4 . (scialert.net)
  • Agriculture sector produces a large amount of by-products and wastes that can be used for cellulase production including rice straw, wheat straw , bean straw, pea pods, corn stover and palm fronds 2 , 10 . (scialert.net)
  • However, it is still unclear, what exactly enhances cellulase production in Rut-C30. (biomedcentral.com)
Substrate3
  • The current findings showed potential possibilities to utilize agricultural wastes as a substrate for producing cellulase enzyme from Penicillium chrysogenum fugal strain which could be an effective additive to improve ruminant diet digestion and utilization. (scialert.net)
  • The molecular weight of the purified cellulase was found to be 67 kDa by SDS-PAGE, had an optimal pH and temperature at 7.0 and 40 °C. According to substrate specificity, the purified cellulase had high specificity on CMC substrate which indicated it to be an endo-β-1,4-glucanase. (biomedcentral.com)
  • Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. (expasy.org)
Bacillus3
  • The isolated strains were identified as Bacillus paralicheniformis and showed strong cellulase activity. (biomedcentral.com)
  • Our whole-genome sequencing classified our three isolates as Bacillus paralicheniformis , and we revealed the presence of ten cellulase orthologues in each of three isolates' genomes. (biomedcentral.com)
  • From our earlier work, we modified the carbohydrate-binding module (CBM) of Bacillus amyloliquefaciens to increase cellulase activity using cold plasma technology. (wu.ac.th)
Vitro1
  • Finally, produced cellulase was evaluated using in vitro batch culture technique. (scialert.net)
Carboxymethyl1
  • The bacterial surface display method was used to selectively screen for improved variants of carboxymethyl cellulase (CMCase). (kribb.re.kr)
Agricultural1
  • seminal cellulases, the furor of agricultural questions Edited in the English form by chickens of the British Isles( commenting Ireland) from the sickly psychoanalysis to the same poem. (beck-68.de)
Genes5
  • However, there is little information regarding cellulase genes in the Red Sea environment. (biomedcentral.com)
  • By whole genome sequencing, we found 10 cellulase genes from the three isolates. (biomedcentral.com)
  • We compared the expression of these cellulase genes under cellulase-inducing and non-inducing conditions and found that most of the cellulase genes were generally upregulated during cellulolysis in the isolates. (biomedcentral.com)
  • Our operon structure analysis also showed that cellulase genes form operons with genes involved in various kinds of cellular reactions, such as protein metabolism, which suggests the existence of crosstalk between cellulolysis and other metabolic pathways in the bacterial isolates. (biomedcentral.com)
  • Our comparative expression analysis also identified that most of the cellulase genes were upregulated under the inducing conditions in general. (biomedcentral.com)
Agar1
  • Ease the gel strength from the resultant cellulase could act on the algal cell wall, promoting the dissolution of agar during hot agar, cellulase could act around the algal cell wall, advertising the dissolution of agar during water extraction. (icbinhibitor.com)
Catalytic1
  • Depending on the number of CBMs appended to the cellulase catalytic core, binding properties toward cellulose and lignin were highly contrasted. (archives-ouvertes.fr)
Https1
  • In brief, the cyst wall was permeabilized by incubating stool specimen in 1 mL of cellulase (Sigma Aldrich, https://www.sigmaaldrich.com ) for 48 hours at 45°C ( 4 ). (cdc.gov)
Cellulolysis1
  • These results suggest that multiple cellulases are playing important roles in cellulolysis. (biomedcentral.com)
Activity3
  • Penicillium chrysogenum recorded the highest value for cellulase activity. (scialert.net)
  • It was derived from the T. reesei wild-type isolate QM6a through three rounds of mutagenesis (ultraviolet (UV) light and N-nitroguanidine) followed by a screening for the release from carbon catabolite repression (CCR) and high cellulase activity [ 8 ]-[ 10 ]. (biomedcentral.com)
  • After cellulase activity was stopped by washing with physiologic water and 5 minutes of centrifugation at 3,000 g, the pellet was incorporated into 4',6-diamidino-2-phenylindole-FISH staining. (cdc.gov)
Strain1
  • Methods: Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain Lactobacillus casei (L. casei) TH 14 and Acremonium cellulase (AC) were used as additives in sorghum silage preparation. (herts.ac.uk)
Complex1
  • 1. Even if research costs are minimal, producing Cellulase is a complex and costly process. (pharmiweb.com)
Isolates2
  • These results suggested that these three isolates secreted active cellulases. (biomedcentral.com)
  • Urbana, IL: University of Illinois Press, 1982, cellulases perfect Conflict and Civic Life: veteres and isolates in India. (beck-68.de)
Agriculture1
  • In such scenario, cellulase is being used in many of the industrial applications mainly but not limited in the field of cotton processing, paper recycling, agriculture and in the field of research and development [2-7]. (cyberleninka.org)
Sugar1
  • Cellulase is a fibre-digesting bone at birth it hellish confusion beyond brain in the Lake Mungo area of Australia dated natural sugar (fibre) in nature cellulose. (dridiesel.com)
Studies2
  • 2. Unlike generic drug creation, Cellulase producers, will also need to invest in clinical studies and safety controls procedures comparable to those performed by original patent-holding firms. (pharmiweb.com)
  • This approach opens an insight for further studies to discovermore about the effects of ILs on cellulase and their interactions in the aqueous system. (iium.edu.my)
Study1
  • In this study, cellulase was therefore tested in several ILs. (iium.edu.my)