Cellulase: An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Cellulose: A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.Trichoderma: A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.Cellulose 1,4-beta-Cellobiosidase: An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.Cellobiose: A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.Carboxymethylcellulose Sodium: A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.Hypocrea: A genus of fungus in the family Hypocreaceae, order HYPOCREALES. Anamorphs include TRICHODERMA.Glycoside Hydrolasesbeta-Glucosidase: An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.Clostridium thermocellum: A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.DextrinsPolyporaceae: A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.Cellulosomes: Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.Clostridium cellulolyticum: A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Endo-1,4-beta Xylanases: Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.Glucan 1,4-beta-Glucosidase: An exocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages of 1,4-beta-D-glucans resulting in successive removal of GLUCOSE units.Glucosidases: Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.Xylans: Polysaccharides consisting of xylose units.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Isoptera: An order of insects, restricted mostly to the tropics, containing at least eight families. A few species occur in temperate regions of North America.Tylenchida: An order of nematodes consisting of many species which are plant parasites. Female worms lay eggs that hatch either in soil or in the host plant.Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Lignin: The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Sporangia: A structure found in plants, fungi, and algae, that produces and contains spores.Neocallimastix: A genus of fungi in the family Neocallimasticaceae, order NEOCALLIMASTIGALES. They contain polyflagellate zoospores and grow on a range of simple and complex carbohydrates in the rumen of sheep and cattle.Actinomycetales: An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.Biomass: Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.Clostridium cellulovorans: A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.Panicum: A plant genus of the family POACEAE. The seed is one of the EDIBLE GRAINS used in millet cereals and in feed for birds and livestock (ANIMAL FEED). It contains diosgenin (SAPONINS).Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Gossypium: A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.Persea: A plant genus in the LAURACEAE family. The tree, Persea americana Mill., is known for the Avocado fruit, the food of commerce.Glucan 1,3-beta-Glucosidase: An exocellulase with specificity for 1,3-beta-D-glucasidic linkages. It catalyzes hydrolysis of beta-D-glucose units from the non-reducing ends of 1,3-beta-D-glucans, releasing GLUCOSE.Gene Expression Regulation, Fungal: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.Glucans: Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Fungal Proteins: Proteins found in any species of fungus.beta-Mannosidase: An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.Fibrobacter: A genus of gram-negative, anaerobic bacteria in the family Fibrobacteraceae, isolated from the human GASTROINTESTINAL TRACT.Endo-1,3(4)-beta-Glucanase: An endocellulase with specificity for the hydrolysis of 1,3- or 1,4-linkages in beta-D-glucans. This enzyme specifically acts on sites where reducing glucose residues are substituted at the 3 position.Polygalacturonase: A cell wall-degrading enzyme found in microorganisms and higher plants. It catalyzes the random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. EC 3.2.1.15.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Ascomycota: A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.Nematoda: A class of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.Schizophyllum: A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Lactose: A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.Penicillium: A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Phanerochaete: A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Bacterial Proteins: Proteins found in any species of bacterium.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Gram-Negative Anaerobic Straight, Curved, and Helical Rods: A group of anaerobic, rod-shaped bacteria that show up as pink (negative) when treated by the Gram-staining method.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

A cold-active glucanase from the ruminal bacterium Fibrobacter succinogenes S85. (1/1106)

We previously characterized two endoglucanases, CelG and EGD, from the mesophilic ruminal anaerobe Fibrobacter succinogenes S85. Further comparative experiments have shown that CelG is a cold-active enzyme whose catalytic properties are superior to those of several other intensively studied cold-active enzymes. It has a lower temperature optimum, of 25 degrees C, and retains about 70% of its maximum activity at 0 degrees C, while EGD has a temperature optimum of 35 degrees C and retains only about 18% of its maximal activity at 0 degrees C. When assayed at 4 degrees C, CelG exhibits a 33-fold-higher kcat value and a 73-fold-higher physiological efficiency (kcat/Km) than EGD. CelG has a low thermal stability, as indicated by the effect of temperature on its activity and secondary structure. The presence of small amino acids around the putative catalytic residues may add to the flexibility of the enzyme, thereby increasing its activity at cold temperatures. Its activity is modulated by sodium chloride, with an increase of over 1.8-fold at an ionic strength of 0.03. Possible explanations for the presence of a cold-active enzyme in a mesophile are that cold-active enzymes are more broadly distributed than previously expected, that lateral transfer of the gene from a psychrophile occurred, or that F. succinogenes originated from the marine environment.  (+info)

Sequence analysis of scaffolding protein CipC and ORFXp, a new cohesin-containing protein in Clostridium cellulolyticum: comparison of various cohesin domains and subcellular localization of ORFXp. (2/1106)

The gene encoding the scaffolding protein of the cellulosome from Clostridium cellulolyticum, whose partial sequence was published earlier (S. Pages, A. Belaich, C. Tardif, C. Reverbel-Leroy, C. Gaudin, and J.-P. Belaich, J. Bacteriol. 178:2279-2286, 1996; C. Reverbel-Leroy, A. Belaich, A. Bernadac, C. Gaudin, J. P. Belaich, and C. Tardif, Microbiology 142:1013-1023, 1996), was completely sequenced. The corresponding protein, CipC, is composed of a cellulose binding domain at the N terminus followed by one hydrophilic domain (HD1), seven highly homologous cohesin domains (cohesin domains 1 to 7), a second hydrophilic domain, and a final cohesin domain (cohesin domain 8) which is only 57 to 60% identical to the seven other cohesin domains. In addition, a second gene located 8.89 kb downstream of cipC was found to encode a three-domain protein, called ORFXp, which includes a cohesin domain. By using antiserum raised against the latter, it was observed that ORFXp is associated with the membrane of C. cellulolyticum and is not detected in the cellulosome fraction. Western blot and BIAcore experiments indicate that cohesin domains 1 and 8 from CipC recognize the same dockerins and have similar affinity for CelA (Ka = 4.8 x 10(9) M-1) whereas the cohesin from ORFXp, although it is also able to bind all cellulosome components containing a dockerin, has a 19-fold lower Ka for CelA (2.6 x 10(8) M-1). Taken together, these data suggest that ORFXp may play a role in cellulosome assembly.  (+info)

Molecular cloning and expression of the novel fungal beta-glucosidase genes from Humicola grisea and Trichoderma reesei. (3/1106)

A novel fungal beta-glucosidase gene (bgl4) and its homologue (bgl2) were cloned from the cellulolytic fungi Humicola grisea and Trichoderma reesei, respectively. The deduced amino acid sequences of H. grisea BGL4 and T. reesei BGL2 comprise 476 and 466 amino acids, respectively, and share 73.1% identity. These beta-glucosidases show significant homology to plant beta-glucosidases belonging to the beta-glucosidase A (BGA) family. Both genes were expressed in Aspergillus oryzae, and the recombinant beta-glucosidases were purified. Recombinant H. grisea BGL4 is a thermostable enzyme compared with recombinant T. reesei BGL2. In addition to beta-glucosidase activity, recombinant H. grisea BGL4 showed a significant level of beta-galactosidase activity, while recombinant T. reesei BGL2 showed weak beta-galactosidase activity. Cellulose saccharification by Trichoderma cellulases was improved by the addition of recombinant H. grisea BGL4.  (+info)

Cellulolytic enzymes in culture filtrates of Rhizoctonia lamellifera. (4/1106)

During growth in a liquid culture containing a single soluble or an insoluble cellulosic carbon source, Rhizoctonia lamellifera released cellulolytic enzymes into the medium. These enzymes were separated by gel filtration and ion-exchange chromatography into seven components, three of high and four of low molecular weight. One of the components had the character of a C1 cellulase. When the components were combined they released more reducing sugars from cellulosic substrates than when used singly.  (+info)

kdgREcc negatively regulates genes for pectinases, cellulase, protease, HarpinEcc, and a global RNA regulator in Erwinia carotovora subsp. carotovora. (5/1106)

Erwinia carotovora subsp. carotovora produces extracellular pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt). The concerted actions of these enzymes largely determine the virulence of this plant-pathogenic bacterium. E. carotovora subsp. carotovora also produces HarpinEcc, the elicitor of the hypersensitive reaction. We document here that KdgREcc (Kdg, 2-keto-3-deoxygluconate; KdgR, general repressor of genes involved in pectin and galacturonate catabolism), a homolog of the E. chrysanthemi repressor, KdgREch and the Escherichia coli repressor, KdgREco, negatively controls not only the pectinases, Pel and Peh, but also Cel, Prt, and HarpinEcc production in E. carotovora subsp. carotovora. The levels of pel-1, peh-1, celV, and hrpNEcc transcripts are markedly affected by KdgREcc. The KdgREcc- mutant is more virulent than the KdgREcc+ parent. Thus, our data for the first time establish a global regulatory role for KdgREcc in E. carotovora subsp. carotovora. Another novel observation is the negative effect of KdgREcc on the transcription of rsmB (previously aepH), which specifies an RNA regulator controlling exoenzyme and HarpinEcc production. The levels of rsmB RNA are higher in the KdgREcc- mutant than in the KdgREcc+ parent. Moreover, by DNase I protection assays we determined that purified KdgREcc protected three 25-bp regions within the transcriptional unit of rsmB. Alignment of the protected sequences revealed the 21-mer consensus sequence of the KdgREcc-binding site as 5'-G/AA/TA/TGAAA[N6]TTTCAG/TG/TA-3'. Two such KdgREcc-binding sites occur in rsmB DNA in a close proximity to each other within nucleotides +79 and +139 and the third KdgREcc-binding site within nucleotides +207 and +231. Analysis of lacZ transcriptional fusions shows that the KdgR-binding sites negatively affect the expression of rsmB. KdgREcc also binds the operator DNAs of pel-1 and peh-1 genes and represses expression of a pel1-lacZ and a peh1-lacZ transcriptional fusions. We conclude that KdgREcc affects extracellular enzyme production by two ways: (i) directly, by inhibiting the transcription of exoenzyme genes; and (ii) indirectly, by preventing the production of a global RNA regulator. Our findings support the idea that KdgREcc affects transcription by promoter occlusion, i.e., preventing the initiation of transcription, and by a roadblock mechanism, i.e., by affecting the elongation of transcription.  (+info)

Characterization of two divergent endo-beta-1,4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit. (6/1106)

Two cDNAs clones (Cel1 and Cel2) encoding divergent endo-beta-1, 4-glucanases (EGases) have been isolated from a cDNA library obtained from ripe strawberry (Fragaria x ananassa Duch) fruit. The analysis of the amino acid sequence suggests that Cel1 and Cel2 EGases have different secondary and tertiary structures and that they differ in the presence of potential N-glycosylation sites. By in vitro translation we show that Cel1 and Cel2 bear a functional signal peptide, the cleavage of which yields mature proteins of 52 and 60 kD, respectively. Phylogenetic analysis revealed that the Cel2 EGase diverged early in evolution from other plant EGases. Northern analysis showed that both EGases are highly expressed in fruit and that they have different temporal patterns of accumulation. The Cel2 EGase was expressed in green fruit, accumulating as the fruit turned from green to white and remaining at an elevated, constant level throughout fruit ripening. In contrast, the Cel1 transcript was not detected in green fruit and only a low level of expression was observed in white fruit. The level of Cel1 mRNA increased gradually during ripening, reaching a maximum in fully ripe fruit. The high levels of Cel1 and Cel2 mRNA in ripe fruit and their overlapping patterns of expression suggest that these EGases play an important role in softening during ripening. In addition, the early expression of Cel2 in green fruit, well before significant softening begins, suggests that the product of this gene may also be involved in processes other than fruit softening, e.g. cell wall expansion.  (+info)

Design of a pH-dependent cellulose-binding domain. (7/1106)

Protein-carbohydrate interactions typically rely on aromatic stacking interactions of tyrosine, phenylalanine and tryptophan side chains with the sugar rings whereas histidine residues are rarely involved. The small cellulose-binding domain of the Cel7A cellobiohydrolase (formerly CBHI) from Trichoderma reesei binds to crystalline cellulose primarily using a planar strip of three tyrosine side chains. Binding of the wild-type Cel7A CBD is practically insensitive to pH. Here we have investigated how histidine residues mediate the binding interaction and whether the protonation of a histidine side chain makes the binding sensitive to pH. Protein engineering of the Cel7A CBD was thus used to replace the tyrosine residues in two different positions with histidine residues. All of the mutants exhibited a clear pH-dependency of the binding, in clear contrast to the wild-type. Although the binding of the mutants at optimal pH was less than for the wild-type, in one case, Y31H, this binding almost reached the wild-type level.  (+info)

Characterization and cloning of celR, a transcriptional regulator of cellulase genes from Thermomonospora fusca. (8/1106)

CelR, a protein that regulates transcription of cellulase genes in Thermomonospora fusca (Actinomycetaceae) was purified to homogeneity. A 6-kilobase NotI-SacI fragment of T. fusca DNA containing the celR gene was cloned into Esherichia coli and sequenced. The celR gene encodes a 340-residue polypeptide that is highly homologous to members of the GalR-LacI family of bacterial transcriptional regulators. CelR specifically binds to a 14-base pair inverted repeat, which has sequence similarity to the binding sites of other family members. This site is present in regions upstream of all six cellulase genes in T. fusca. The binding of CelR to the celE promoter is inhibited specifically by low concentrations of cellobiose (0.2-0.5 mM), the major end product of cellulases. The other sugars tested did not affect binding at equivalent or 50-fold higher concentrations. The results suggest that CelR may act as a repressor, and that the mechanism of induction involves a direct interaction of CelR with cellobiose.  (+info)

Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill. Luis A. Ordaz-Díaz,a Juan A. Rojas-Contreras,b Felipe Flores-Vichi,δc Mónica Y. Flores-Villegas,a Carlos Álvarez-Álvarez,a Pryscila Velasco-Vázquez,a and Ana M. Bailón-Salas b,*. The aim of this study was to identify cellulolytic fungal strains capable of degrading cellulose from an aerated lagoon in a pulp and paper mill. Four fungal strains that were found to be highly active were isolated on carboxymethyl cellulose (CMC) and suggested to be CMCase/endoglucanase. The identified strains were Aspergillus niger, Penicillium sp.,Aspergillus fumigatus, and Mucor sp. All the strains were studied in terms of cultural morphological characteristics and microscopic examinations. The endoglucanase with the highest isolate production was Penicillium sp., which also showed the highest qualitative endoglucanase activity (1.3 cm), in addition to the main ...
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Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic ...
Results: It was observed that cellulase activity of T. reesei strains varies from 0.73 to 3.11 IU and T. reesei 5A shown maximum cellulase activity after 96 h of growth perod at 37 °C. Total genomic DNA was isolated from T. reesei 5A and then it was partially digested with Sau 3A and ligated to vector YEpFLAG-1, linearized with BamH1. The construct was used to transform Escherichia coli and recombinant clone(s) were screened on Reese medium supplemented with carboxymethyl cellulose and ampicillin. The E. coli recombinant clones were further confirmed by gene specific amplification using PCR. S. cerevisiae was transformed with the recombinant plasmids YEpFLAG-1-cel-exo and YEpFLAG-1-cel-endo isolated from E. coli transformants. Carboxymethyl cellulase (CMCase) activity was observed in three yeast transformants Tr-2, Tr-4 and Tr-6 as 0.50, 0.70 and 0.80 IU, respectively. no FPase (exoglucanse) activity was observed in any of the yeast transformants ...
Allcosmeticsource.com Cellulase 100,000u/g,1kg/bag,free shipping [EP170508014]- Cellulase 100,000u/g,1kg/bag,free shipping What is Cellulase 100,000u/g Cellulase refers to the total name of multiple enzymes which can catalyze and hydrolyze cellulose. Generally, cellulase which can hydrolyze natural cellulose contains three activity constituents: endoglucanase, exoglucanase and glucuroide. Function of Cellulase 100,000u/g (1) The effect of snowflake, stereoscopic and color brightness can be further improved (2)
Disclosed are improved methods for treating cotton-containing fabrics as well as the fabrics produced from these methods. In particular, the disclosed methods are directed to contacting cotton-containing fabrics with a cellulase solution containing a fungal cellulase composition which is substantially,free of all CBH I type cellulase components. Cotton-containing fabrics so treated possess decreased strength loss as compared to fabrics treated with a cellulase solution containing a complete cellulase composition.
Deletion of Cel48S from C. thermocellum led to a decrease in the enzymatic hydrolysis rate, a decrease in microbial hydrolysis rate, and a decrease in biomass formation during growth on Avicel.. The similarity of enzyme saturation curves for the WT and parent strains suggests that the ΔpyrF mutation in the parent strain has no effect on cellulosome function, as expected. The S mutant strain, however, exhibited a reduction in both specific activity and saturation rate. A reduction in specific activity is indicative of impaired function and consistent with decreased synergy among components of the cellulosome in the absence of Cel48S (3).. The role of GH families in cellulose solubilization is a topic of much debate. Family 48 cellulases are a prominent component of many bacterial cellulase systems and, due to their ubiquity, are thought to play an important role in cellulose solubilization (21). On one hand, disruption of the single family 9 GH in C. phytofermentans eliminated its ability to ...
Cellulases have numerous applications in several industries, including biofuel production, food and feed industry, brewing, pulp and paper, textile, laundry, and agriculture.Cellulose-degrading bacteria are widely spread in nature, being isolated from quite different environments. Cellulose degradation is the result of a synergic process between an endoglucanase, an exoglucanase and a,β-glucosidase. Bacterial endoglucanases degrade ß-1,4-glucan linkages of cellulose amorphous zones, meanwhile exoglucanases cleave the remaining oligosaccharide chains, originating cellobiose, which is hydrolyzed by ß-glucanases. Bacterial cellulases (EC 3.2.1.4) are comprised in fourteen Glycosil Hydrolase families. Several advantages, such as higher growth rates and genetic versatility, emphasize the suitability and advantages of bacterial cellulases over other sources for this group of enzymes. This review summarizes the main known cellulolytic bacteria and the best strategies to optimize their cellulase
cellulase for bio polishing Manufacturers & cellulase for bio polishing Suppliers Directory - choose cellulase for bio polishing from 183 cellulase for bio polishing Super Suppliers of China.
A novel hemicellulase-producing fungal strain was isolated from a local soil sample. The organism is identified as Aspergillus fumigatus based on ribosomal RNA analyses. The Aspergillus strain, designated as 2NB, produces both enzymes acting on xylan backbone (xylanase and β-xylosidase), and those acting on side chains (or accessory enzymes) notably α-arabinofuranosidase and acetyl-xylan esterase. The Asperigillus hemicellulases are characterized as having relatively low xylanase and β-xylosidase activities but high side chain removal activities. The activity ratio of side-chain acting enzymes to xylanase is higher than that of the Multifect enzyme, a commercial hemicellulase product. The potential of the novel hemicellulases in lignocelluloses bioprocessing was demonstrated with alkaline-pretreated switchgrass as lignocellulose substrate with hemicellulase supplemented with a ratio of xylanase activity to filter paper unit of 2:1. Supplement of Aspergillus hemicellulases to commercial ...
Utilization of cellulases as substitute of chemical process gained huge momentum in the field of biotechnology Now there is dire need to find out un explore reveres of fungi possessing greater potential for efficient cellulase production. This boosted isolation of novel thermo tolerant fungal strains capable of producing the targeted product. In this investigation 70 thermophilic cellulolytic fungal strains were isolated. All the strains were screened via submerged fermentation. The strain showing highest CMCase activity was identified by conventional method i.e. based on morphology and microscopic features and confirmed by 18S rDNA gene sequencing, using specific ITS primers. The modified CTAB method was used for rapid extraction of DNA from thermo tolerant strain. The selected strain subsequently subjected to sequencing and phylogenetic analysis. The result indicates the selected strain was found to be T. dupontii. For strain improvement the T. dupontii was subjected to random mutagenesis by ...
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The structures of the Glu140--|Gln mutant of the Clostridium thermocellum endoglucanase CelC in unliganded form (CelC(E140Q)) and in complex with
Several alkalophilic Bacillus spp. strains were selected for their capacity to produce alkaline cellulases. Culture supernatants of these strains showed optimal cellulase activities between pH 8 and 9 and they were stable from pH 6 to pH 12. A cellul
Summary of Facts and Submissions. I. The appeal lies from a decision of the Opposition Division revoking European patent 0 699 248, granted on European application No. 95 913 647.4.. II. The application as filed comprised 8 claims, reading, respectively, as follows:. 1. In a method for enhancing the feel and/or appearance and/or for providing color enhancement to a non-cotton containing cellulosic fabrics during manufacture of the fabric by treatment of the fabric with a composition comprising a naturally complete fungal cellulase composition which comprises exo-cellobiohydrolase type component(s) and endoglucanase type component(s) wherein the improvement comprises modifying the naturally complete fungal cellulase composition to comprise at least 10 weight percent of endoglucanase type components based on the total weight of protein in the fungal cellulase composition and be free of all CBH I type cellulase components.. 2. The method according to Claim 1 wherein said fungal cellulase ...
Non-cellulosomal processive endoglucanase 9I (Cel9I) from Clostridium thermocellum is a modular protein, consisting of a family-9 glycoside hydrolase (GH9) catalytic module and two family-3 carbohydrate-binding modules (CBM3c and CBM3b), separated by linker regions. GH9 does not show cellulase activity when expressed without CBM3c and CBM3b and the presence of the CBM3c was previously shown to be essential for endoglucanase activity. Physical reassociation of independently expressed GH9 and CBM3c modules (containing linker sequences) restored 60-70% of the intact Cel9I endocellulase activity. However, the mechanism responsible for recovery of activity remained unclear. In this work we independently expressed recombinant GH9 and CBM3c with and without their interconnecting linker in Escherichia coli. We crystallized and determined the molecular structure of the GH9/linker-CBM3c heterodimer at a resolution of 1.68 Å to understand the functional and structural importance of the mutual spatial orientation
Hyper-Productivity, Characterization, and Exploitation of a Cellulase Complex from a Novel Isolate of Aspergillus tubingenesis S2 using Lignocellulose-based Material
Effect of pH on the activity of (■) free cellulase, (●) immobilized cellulase, and (▲) immobilized cellulase + ionic liquid. Morphologies of immobilized c
Cellulase produced byTrichoderma viride acted on carboxymethyl cellulose with a Km of 4.9 g substrate per litre, showing a pH optimum at 4.5 and a temperature optimum at 55 °C. Ag+, Hg2+, Zn2+, Cu2+ and N3- were inhibitory.
glucosidases. A gene encoding endoglucanase, designated as cel12, was cloned from total RNA prepared from F. palustris grown at the expense of Avicel. The gene encoding Cel12 has an open reading frame of 732 bp, encoding a putative protein of 244 amino acid residues with a putative signal peptide residing at the first 18 amino acid residues of the N-terminus of the protein. Sequence analysis of Cel12 identified three consensus regions, which are highly conserved among fungal cellulases belonging to GH family 12. However, a cellulose-binding domain was not found in Cel12, like other GH family 12 fungal cellulases. Northern blot analysis showed a dramatic increase of cel12 mRNA levels in F. palustris cells cultivated on Avicel from the early to late stages of growth and the maintenance of a high level of expression in the late stage, suggesting that Cel12 takes a significant part in endoglucanase activity throughout the growth of F. palustris. Adventitious expression of cel12 in the yeast Pichia ...
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored...
The microbial degradation of cellulose and xylans requires several types of enzyme such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [(PUBMED:1886523)]. Structurally, cellulases and xylanases generally consist of a catalytic domain joined to a cellulose-binding domain (CBD) by a short linker sequence rich in proline and/or hydroxy-amino acids.. The CBD domain is found either at the N-terminal or at the C-terminal extremity of these enzymes. As it is shown in the following schematic representation, there are two conserved cysteines in this CBD domain - one at each extremity of the domain - which have been shown [(PUBMED:1761039)] to be involved in a disulphide bond. There are also four conserved tryptophan, two are involved in cellulose binding. The CBD of a number of bacterial cellulases has been shown to consist of about 105 amino acid residues [(PUBMED:1812490), (PUBMED:10973978)].. ...
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Hemicellulase is used to clarify the juice of mandarin oranges. Although pectinase is used to clarify the juices of apples and grapes, the juice of mandarin oranges are clarified by the simultaneous use of pectinase and hemicellulase. In food processing, it is rare that the desired result can be obtained with the use of hemicellulase alone. Hemicellulases work in conjunction with cellulases and pectinases ...
Paper sludge is the largest solid waste stream produced by pulp and paper industry, and is also an attractive feedstock for emergent technologies based on processing of cellulosic biomass featuring enzymatic hydrolysis. This study focuses on investigating conversion of paper sludge to ethanol under industrially relevant conditions. A solids-fed simultaneous saccharification and fermentation laboratory reactor system capable of aseptic, semi-continuous metered delivery of paper sludge was developed to carry out experiments with hydrolysis mediated by commercial cellulase preparations and fermentation of glucose to ethanol mediated by Saccharomyces cerevisiae. Economically recoverable concentrations of ethanol were produced, and good material balance closure was achieved. Decreasing feeding frequency (feed additions per residence time) was found to allow the cellulase loading to be decreased at least two-fold with no decrease in cellulose conversion. Although decreasing feeding frequency results ...
The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family D [3] or as the glycosyl hydrolases family 8 [4,E1]. The enzymes which are currently known to belong to this family are listed below. ...
Books and Book Chapters. 1. Lactase production by Aspergillis Oryzae , Lambert publications, Germany, 2012. 2. Cellulase production by Aspergillus niger , Lambert publications , Germany, 2012. 3. Biological synthesis, Characterization and antimicrobial activity of silver nanoparticles from beetle leaves, In Nanoscience and drugdelivery. Apple science publication USA. Projects:. 1. Major Research project on Cellulase production by mutant fungal strain Aspergillus niger Sanctioned by University Grants Commision 2009. New Delhi, India. Abstracts. Indian Science Abstracts:. ...
The present invention provides a novel cellulase composition obtainable from Bacillus sp. CBS 669.93. A preferred cellulase has a calculated molecular weight of approximately 63 kD, a calculated isoelectric point of about 5 and a pH optimum on CMC of about 6 at 40 C. and 60 C.
Cellualse is one of the most important enzymes used in textile, detergent, paper, food and feed industries. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples. 24 soil samples were analyzed and 54 presumptive Bacillus isolates were isolated after heating the soil samples at 80°C for 10 min. Among them 45 isolates showed enzyme activity ranging from 0.003 to 0.17 U/ml in test tubes containing 5 ml medium composed of (g/L) glucose 0.5 gm, peptone 0.75 gm, FeSO4 0.01 gm, KH2PO4 0.5 gm, and MgSO4 0.5 gm at 120 rpm, 37° C and pH 7. Among them 1RW, 2WS, 3YR, 4WT, 6 RR, and 9SS showed 0.17, 0.15, 0.14, 0.15, 0.147 and 0.14U/ml enzyme activities, respectively. Production of cellulase by these isolates was further scaled up to shake culture containing 50 ml medium similar to that used in test tube culture. Among the isolates 1 RW showed the maximum activity. This 1 RW was identified by API kit and showed that 59 % belongs to ...
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Omega Pharma Cellulase Anticellulite Mousse 150ml. Cellulase Crackling Mousse is a cellulite treatment that improves skin vasodilation by stimulating the microcirculation, improves penetration of active ingredients and provides a pleasant feeling of...
Cellulase is often a plant enzyme that aids during the digestion of fibrous substances. Cellulase is utilized as a digestive aid and for the management of flatulence.Cellulase Cellulase is surely an enzyme which breaks down cellulose to beta-glucose. Human beings will not make cellulase of their bodies, and so are therefore struggling to use a lot … Read More. ...
Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.
endo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.- ...
endo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.- ...
Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression
Spratling, B.M., R.D. Wiedmeier, D.R. ZoBell, C.A. Fitzgerald, and B.A. Kent. 2001. Exogenous cellulase enhances the utilization of ammoniated wheat straw in pregnant and lactating beef cows. Proceedings, Western Section, American Society of Animal Science. Vol. 52:349-352.. ...
Read "Expression and import of an active cellulase from a thermophilic bacterium into the chloroplast both in vitro and in vivo, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related pol
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related pol
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Produced primarily by fungi, bacteria, and protozoans to catalyze cellulolysis, or the decomposition of cellulose and its related polysaccharides.
Shell said it will pay Codexis $7.5m for the remaining full-time employee equivalents (FTEs) and milestone payments that would have been due under the original agreement. Shell also agreed not to sell any cellulase enzymes to third party biofuel customers using technology developed by Codexis after the end of the Shell Research deal.. Shell retains its right to use and manufacture the enzymes including those enzymes that result from Codexis development in the span of a ten-year period beginning August 31, 2012. The enzymes will be for Shells and its affiliates own use only. Shell can also sub-license the right to manufacture the enzymes to third parties but only for Shells own use.. Codexis, on the other hand, was able to get a royalty-bearing, non-exclusive license to develop, manufacture, use and sell cellulase enzymes for biofuels use worldwide (except in Brazil) developed during the companies research collaboration since November 1, 2006. Codexis, however, has the exclusive rights to ...
Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). According to sequence and protein domain analyses, EGLB was assigned to glycosyl hydrolase family 5 of the cellulase superfamily. Several binding sites were found in the promoter region. The purified recombinant enzyme was induced by 0.5% methanol, and it exhibited optimal activity at 70 °C and pH 4. EGLB was stable for 3 h at temperatures below 60 °C, with more than 90% of its activity remaining. The enzyme was specific for substrates with β-1,3 and β-1,4 linkages. In Lineweaver-Burk plot analysis, the Km and Vmax values of EGLB for β-D-glucan were 134 mg/mL and 4.68 U/min/mg, respectively. The enzyme activity was increased by 1.86-fold by Co2+ and by 2-fold by Triton X-100 and
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A method for preparing an aqueous solution enriched in EG III from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The method involves adding an amount of a low molecular weight alcohol selected from the group consisting of ethanol, methanol, propanol and mixtures thereof to the aqueous mixture containing cellulase proteins, xylanase and EG III and an organic salt under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The method then involves removing the precipitate from the aqueous mixture so as to recover an aqueous supernate enriched in EG III. Next, the method involves adding an amount of an inorganic salt to the supernate produced in step b) so as to form a second precipitate and a second supernate and then finally collecting the second supernate from the second precipitate to obtain a supernate enriched in EG III.
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The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Candex is a potent cellulase enyzme product formulated specifically for the treatment of Candida albicans. Since the cell wall of candida are mostly made up of fiber, digestive enyzmes such as cellulase and hemicellulase can effectively kill candida by breaking it down. Candex does not cause herxheimer (or die-off) reaction and yeast cannot become resistant to this type of treatment. Fiber digesting enzymes are safe and work most effectively when .... Continue Reading → ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
The present invention provides an endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
Thermal stability of FPase (triangles), endoglucanase (squares), and β-glucosidase (circles) produced by T. harzianum at 37°C (black and white symbols), 50°C
Video articles in JoVE about thermotoga maritima include FtsZ Polymerization Assays: Simple Protocols and Considerations, Protein Crystallization for X-ray Crystallography, High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli.
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Macerating enzyme for premium reds Lallzyme EX-V TM is a pectinase with cellulase and hemicellulase side activities for red wines destined for aging. It has a specific action on both grape cell walls and cell membranes. This action allows for a complete and rapid release of anthocyanins and a more efficient release of tannins leading to stable anthocyanin-tannin bonding. The end result of this bonding is a more
Exposure of cellulosic biomass to temperatures of about 120-210°C can remove most of the hemicellulose and produce cellulose-rich solids from which high glucose yields are possible with cellulase enzy
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Infinite Enzymes produces cellulases for converting plant biomass into sugars for fermentation into ethanol or other biobased products.
The present invention relates to the production of edible wafers having a density of at least 35 mg/cm3 the wafer comprising (i) 100 parts by weight of a flour; (ii) a low amount of water, in an amount so the weight ratio of total amount of water to total amount of flour in the batter (denoted herein as R[w/f]) is no more than 0.8; and (iii) at least one enzyme comprising a cellulase in an amount of at least 0.0001 parts by weight; where the batter has a viscosity of from 200 to 1900 cps so it can be both pumped and baked on a heated surface without spillage.
Concentrated multi-enzyme liquid blend containing lipase, amylase and cellulase which can remove various types of stains when used in low alkali pH detergent formulation. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Several significant hits in PSI-BLAST to predicted endoglucanases, but far more hits to hypothetical protein sequences. E.g. residues 14-345 are 32% similar to Y555_METJA, a hypothetical protein. Residues 6-347 are 33% similar to GUNM_CLOTH, an englucanase ...
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An analysis of the recently published genome sequence of Cytophagahutchinsonii revealed an unusual collection of genes for an organism that can attackcrystalline cellulose. Consequently, questions were being raised by cellulase scientists, as towhat mechanism this organism uses to degrade its insoluble substrates. Cellulose, being ahighly polymeric compound and insoluble in water, cannot enter the cell walls ofmicroorganisms. Cellulose-degrading enzymes have therefore to be located on the surface ofthe cell wall or released extracellularly. The location of most cellulase enzymes has beenstudied. However, basic information on C. hutchinsonii cellulases is almost non-existent. Inthe present study, the location, formation and biosynthetic regulation of cellulases in C.hutchinsonii were demonstrated on different substrates. Various fractions isolated from C.hutchinsonii after cell rupture were assayed for carboxymethyl-cellulase activity (CMC).The cellulases were found to be predominantly cell-free during
Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may be used for the production of industrial materials from plant biomass. These enzymes may also improve the fiber properties of cotton for manufacturing or clothes. The simple sugars ...
Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) [ME-CELBA] - High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. EC 3.2.1.4 CAZy Family: GH5 Recombinant. From Bacillus amyloliquefaciens. In 3.2 M ammonium sulphate. Specific activity: ~ 80 U/mg (40oC, pH 6.0 on CM-cellulose 4M); ~ 160 U/mg (60oC, pH 6.0 on CM-cellulose 4M). Stability: | 2 years at 4oC.
Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases) enzymes. One approach to separating these enzymes is affinity chromatography. A new ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose. The property of APTC in affinity chromatography is demonstrated using Trichoderma reesei cellulases. The behavior of these enzymes on APTC-affinity column was essentially equivalent to that reported for the same enzymes on p-aminobenzyl 1-thio-β- D-cellobioside (ABTC)-columns; ABTC being the traditional ligand for affinity chromatography of exocellulases. The primary advantage of the APTC ligand is its ease of preparation. The affinity between CBHs and APTC may be considerably affected by nonspecific interactions. In this study, the significance of nonspecific protein/matrix interactions in affinity chromatography of cellulolytic enzymes is evaluated. The role of pH, ...
Effect of Natural and Pretreated Soybean Hulls on Enzyme Production by Trichoderma reesei. (A. M. Coffman, Q. Li, L.-K. Ju) Journal of the American Oil Chemists Society 91 (8), 1331-1338 (2014). View Article. Promoting Pellet Growth of Trichoderma reesei Rut C30 by Surfactants for Easy Separation and Enhanced Cellulase Production. (N.V. Callow and L.-K. Ju) Enzyme and Microbial Technology 50(6-7), 311-317 (2012). View Article. Cellulase production by continuous culture of Trichoderma reesei Rut C-30 using acid hydrolysate prepared to retain more oligosaccharides for induction. (C.-M. Lo, Q. Zhang, N. V. Callow and L.-K. Ju) Bioresource Technology. 101(2), 717-23 (2010). View Article. Cell immobilization with polyurethane foam for retaining Trichoderma reesei cells during foam fractionation for cellulase collection. (Q. Zhang, C.-M. Lo, and L.-K. Ju) Applied Biochemistry and Biotechnology 156, 12-23 (2009). View Article. Cellulase production by cocultures of Hypocrea jecorina Rut C30 and Candida ...
phdthesis{a42f4c58-3e50-41b9-87ac-a3c37465f8bc, abstract = {The enzymatic degradation of wood polysaccharides such as cellulose and hemicellulose is an important process in nature. In addition, cellulases and hemicellulases can be used in industrial applications. Fuel ethanol can potentially be produced from wood by enzymatic hydrolysis of cellulose followed by yeast fermentation of the formed sugars.,br/,,br, ,br/,,br, In this thesis, fungal glycoside hydrolases, cellulases and hemicellulases were studied with the aim of increasing our knowledge of the mechanisms involved in the enzymatic hydrolysis of cellulose and lignocellulose. The focus was mainly on cellulases from the filamentous fungus Trichoderma reesei. However, lignocellulose also contains hemicellulose and studies of hemicellulases are included,br/,,br, ,br/,,br, In Paper I-IV the mechanisms involved in cellulose degradation were investigated. Features of enzymatic cellulose hydrolysis such as synergism, decreasing hydrolysis rate ...
The interaction between cellulase enzymes and their substrates is of central importance to several technological and scientific challenges. Here we report that the binding of cellulose binding modules (CBM) from Trichoderma reesei cellulases Cel6A and Cel7A show a major difference in how they interact with substrates originating from wood compared to bacterial cellulose. We found that the CBM from TrCel7A recognizes the two substrates differently and as a consequence shows an unexpected way of binding. We show that the substrate has a large impact on the exchange rate of the studied CBM, and moreover, CBM-TrCel7A seems to have an additional mode of binding on wood derived cellulose but not on cellulose originating from bacterial source. This mode is not seen in double CBM (DCBM) constructs comprising both CBM-TrCel7A and CBM-TrCel6A. The linker length of DCBMs affects the binding properties, and slows down the exchange rates of the proteins and thus, can be used to analyze the differences ...
Non-specific binding of cellulases to lignin has been implicated as a major factor in the loss of cellulase activity during biomass conversion to sugars. It is believed that this binding may strongly impact process economics through loss of enzyme activities during hydrolysis and enzyme recycling scenarios. The current model suggests glycoside hydrolase activities are lost though non-specific/non-productive binding of carbohydrate-binding domains to lignin, limiting catalytic site access to the carbohydrate components of the cell wall. In this study, we have compared component enzyme affinities of a commercial Trichoderma reesei cellulase formulation, Cellic CTec2, towards extracted corn stover lignin using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and p-nitrophenyl substrate activities to monitor component binding, activity loss, and total protein binding. Protein binding was strongly affected by pH and ionic strength. β-d-glucosidases and xylanases, which do not have carbohydrate
Various enzymatic cocktails were produced from two Trichoderma reesei strains, a cellulase hyperproducer strain and a strain with β-glucosidase activity overexpression. By using various carbon sources (lactose, glucose, xylose, hemicellulosic hydrolysate) for strains growth, contrasted enzymatic activities were obtained. The enzymatic cocktails presented various levels of efficiency for the hydrolysis of cellulose Avicel into glucose, in presence of xylans, or not. These latter were also hydrolyzed with different extents according to cocktails. The most efficient cocktails (TR1 and TR3) on Avicel were richer in filter paper activity (FPU) and presented a low ratio FPU/β-glucosidase activity. Cocktails TR2 and TR5 which were produced on the higher amount of hemicellulosic hydrolysate, possess both high xylanase and β-xylosidase activities, and were the most efficient for xylans hydrolysis. When hydrolysis of Avicel was conducted in presence of xylans, a decrease of glucose release occurred for all
Plasma-assisted pretreated wheat straw was investigated for cellulase and xylanase production by Trichoderma reesei fermentation. Fermentations were conducted with media containing washed and unwashed plasma-assisted pretreated wheat straw as carbon source which was sterilized by autoclavation. To account for any effects of autoclavation, a comparison was made with unsterilized media containing antibiotics. It was found that unsterilized washed plasma-assisted pretreated wheat straw (which contained antibiotics) was best suited for the production of xylanases (110 IU ml(-1)) and cellulases (0.5 filter paper units (FPU) ml(-1)). Addition of Avicel boosted enzyme titers with the highest cellulase titers (1.5 FPU ml(-1)) found with addition of 50 % w/w Avicel and with the highest xylanase production (350 IU ml(-1)) reached in the presence of 10 % w/w Avicel. Comparison with enzyme titers from other nonrefined feedstocks suggests that plasma pretreated wheat straw is a promising and suitable ...
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. The name is also used for any naturally occurring mixture or complex of various such enzymes, that act serially or synergistically to decompose cellulosic material. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. Cellulose breakdown is of considerable economic importance, because it makes a major constituent of plants available for consumption and use in chemical reactions. The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal beta-D-glucans. Because cellulose molecules bind strongly to each other, cellulolysis is relatively difficult compared to the breakdown of other polysaccharides such as starch. Most mammals have only very ...
Method of preparation and some properties of amorphous cellulose nanoparticles (ANP) have been described in this paper. It was shown that ANP have spherical shape and are characterized by high degree of pantamorphia, low DP and increased content of sulfonic groups. The amorphous nanoparticles of cellulose are completely hydrolyzed by cellulolytic enzymes with forming of glucose. Concentrated paste of ANP has expressed thickening properties and therefore its additive can prevent phase separation of water dispersions of various substances. Low-acidic and soft nanoparticles can be used in cosmetic formulation for gentle skin peeling. Moreover, due to increased content of acidic functional groups, ANP can immobilize various therapeutically-active substances (TAS) containing basic functional groups. The ANP-TAS complexes can be used in remedies aimed for effective care and cure of the skin.
Background The in-depth understanding of the enzymatic hydrolysis of cellulose with heterogeneous morphology (that is, crystalline versus amorphous) may help develop better cellulase cocktail mixtures and biomass pretreatment, wherein cost-effective release of soluble sugars from solid cellulosic materials remains the largest obstacle to the economic viability of second generation biorefineries. Results In addition to the previously developed non-hydrolytic fusion protein, GC3, containing a green fluorescent protein (GFP) and a family 3 carbohydrate-binding module (CBM3) that can bind both surfaces of amorphous and crystalline celluloses, we developed a new protein probe, CC17, which contained a mono-cherry fluorescent protein (CFP) and a family 17 carbohydrate-binding module (CBM17) that can bind only amorphous cellulose surfaces. Via these two probes, the surface accessibilities of amorphous and crystalline celluloses were determined quantitatively. Our results for the enzymatic hydrolysis of ...
Hydrolysis of cellulose to glucose is the most critical step in bioconversion of lignocellulosic biomass to fuels and chemicals. Cellulases and homogenous acids are widely used for cellulose hydrolysis. However, cellulases can only be used at moderate conditions and need longer time to achieve satisfactory cellulose hydrolysis. Homogenous acids can tolerate higher temperature, but they have limitations and issues such as equipment corrosion, recycling and wastewater treatment. To address these issues, heterogeneous solid acids have recently drawn a lot of attention for hydrolyzing cellulose. Traditional solid acids such as sulfonated carbon and resins, however, are not as effective as homogenous acids and cellulases in hydrolyzing cellulose because they have poor access/affinity to cellulose. In this study, a series of porous polymeric solid acids were synthesized for cellulose hydrolysis. These cellulase-mimetic solid acids have hydroxyl, halide, or boronic acid as cellulose-binding group in ...
Abdulrahman AO, Huisingh D (2018). The role of biomass as a cleaner energy source in Egypts energy mix. Journal of Cleaner Production 172:3918-3930. Ahmed S, Bashir A, Saleem H, Mubshara S, Jamil A (2009). Production and purification of cellulose - degrading enzymes from a filamentous fungus. Pakistan Journal of Botany 41(3):1411-1419. Bakker RRC, Elbersen HW, Poppens RP, Lesschen JP (2013). Rice straw and wheat straw. Potential feedstocks for the biobased economy. NL Agency. Report No. 448025. Retrieved from https://library.wur.nl/WebQuery/wurpubs/448025. Bayer EA, Morag E, Lamed R (1991). The cellulosome - a treasure- trove for biotechnology. Journal of Biological Chemistry 266(253):9241-9250. Béguin P, Aubert J-P (1994). The biological degradation of cellulose. Federation of European Microbiological Societies Microbiology Reviews 13(1):25-58. Benoit I, Culleton H, Zhou M, Difalco M, Osorio GA, Battaglia E, … Vries R P (2015). Closely related fungi employ diverse enzymatic strategies to ...
Trichoderma reesei is a key fungus for industrial production of lignocellulolytic enzymes. The genome sequences of the T. reesei hyper-cellulolytic strain RUT-C30 and its parental strain QM6a were compared at the nucleotide level. Approximately 97% of the 87 genomic-sequence scaffolds of T. reesei QM6a (33Mb) were found to have the corresponding nucleotide in the 182 genome-sequence scaffolds of RUT-C30 (32Mb). There are 455 loci within the QM6 sequence not detected in the RUT-C30 sequence. Regions at the termini of QM6a scaffolds as well as 14 small scaffolds do not have corresponding regions in RUT-C30 genomic scaffolds. Seventy-eight protein-encoding genes are included within these regions. Mutated nucleotide(s) in 2,371 positions, including short insertion/deletions (indels), were detected in the aligned regions. The predicted protein-coding regions of 97 gene models contain mutations, 34 of which were not previously described. Twenty-seven out of 34 newly discovered genes were found to have ...
The 2018 Gordon Research Seminar on Cellulosomes, Cellulases and Other Carbohydrate Modifying Enzymes (GRS) will be held in Andover, NH. Apply today to reserve your spot.
Meruliporia incrassata ATCC ® 11236™ Designation: Madison 563 Application: fungus resistance testing produces endoglucanase Cel 25 produces endoglucanase Cel 49 produces endoglucanase Cel 57 testing wood preservatives
One.Product presentation Cellulase is extracted from the fermentation solution which is produced by fermenting Trichoderma koningii which is a fruitful strain. This enzyme is being used in textile,feed,alcohol,fuel alcohol,beer fermentation,extract medicine,etc. Two.Product specification and quality index Item International Trade Leads - Importers And Exporters. INTERNATIONAL TRADE DIRECTORY for b2b trade. Suppliers and business opportunities in USA, EUROPE, ASIA and around the world..
... produces cellulase. Da Vinha, FN; Gravina-Oliveira, MP; Franco, MN; Macrae, A; da Silva Bon, EP; ... "Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic biomass as inducer substrate". Applied ... Nascimento, RP; Coelho, RR (June 2011). "Cellulase production by Streptomyces viridobrunneus SCPE-09 using lignocellulosic ...
The cellulase family of enzymes have a one to two order smaller magnitude of efficiency. Therefore, it requires 40 to 100 times ... For cellulase produced offsite, enzyme production amounts to 36% of cash cost. For enzyme produced onsite in a separate plant, ... Cellulases and hemicellulases used in the production of cellulosic ethanol are more expensive compared to their first ... A recent paper estimates the range at 13-36% of cash costs, with a key factor being how the cellulase enzyme is produced. ...
... cellulases; lipases; xylanases; pectinases; chitinases and their metabolites, including: 2-phenylamine; carotenoids; ...
Cellulase activities of Ulocladium chartarum were found to be influenced by the mode of incubation as well as by the age of the ... CS1 maint: Extra text (link) Sallam, Lotfy; H.El-Refai, Abdel-Monem; El-Sayed, Samia M. (1988). "Cellulase production by ... The role of some cultural conditions on cellulase production by Ulocladium chartarum". Zentralblatt für Mikrobiologie. 143 (8 ...
The cellulase activity of C. globosum functions best at temperatures ranging from 25-32 degrees Celsius and is stimulated by ... Cellulase is inhibited by ethyl malonate. Like many fungal species, C. globosum obtains their energy from carbon sources such ... Like most Chaetomium species, C. globosum decomposes plant cells using hyphal cellulase activity. Even though they are known to ...
"A cellulase gene of termite origin". Nature. 394 (6691): 330-31. doi:10.1038/28527. PMID 9690469. Retrieved 16 May 2017. ...
It is a source of cellulase. 5-Hydroxymaltol and mycophenolic acid are substances that can be found in P. echinulatum. " ... "Comparison of Penicillium echinulatum and Trichoderma reesei cellulases in relation to their activity against various ...
One soft-rot Ascomycete, Trichoderma reesei, is used extensively in industrial applications as a source for cellulases and ... Wilson, David B (2009). "Cellulases and biofuels". Current Opinion in Biotechnology. 20 (3): 295-299. doi:10.1016/j.copbio. ... Extracellular enzymes target macromolecules such as carbohydrates (cellulases), lignin (oxidases), organic phosphates ( ... production of extracellular enzymes and characterization of the major cellulases". Microbiology. 152 (12): 3613-3622. doi: ...
One of these families is known as the cellulase family F or as the glycosyl hydrolases family 10. Henrissat B, Callebaut I, ... Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon JP (September 1989). "Cellulase families revealed by hydrophobic cluster ... cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. ... These enzymes were formerly known as cellulase family F. The microbial degradation of cellulose and xylans requires several ...
The mutant Pol6 produces a very high amount of cellulase and pectinase. This mutant might be used for industrial use. Belghith ... Ellouz Chaabouni, S.; Belguith, H.; Hassairi, I.; m'Rad, K.; Ellouz, R. (1995). "Optimization of cellulase production by ... Jain, S.; Parriche, M.; Durand, H.; Tiraby, G. (1990). "Production of polysaccharidases by a cellulase-pectinase hyperproducing ... Belghith, H.; Ellouz-Chaabouni, S.; Gargouri, A. (2001). "Biostoning of denims by Penicillium occitanis (Pol6) cellulases". ...
Harchand, R. K.; Singh, S (1994). "Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus". Journal of ... Harchand, R. K.; Singh, S (1997). "Extracellular cellulase system of a thermotolerant streptomycete: Streptomyces albaduncus". ... "Characterization of cellulase complex of Streptomyces albaduncus". Journal of basic microbiology. 37 (2): 93-103. PMID 9151422 ...
Most animals, however, do not produce cellulase; the enzyme needed to digest cellulose. However some animal and species have ... and cellulase. These enzymes improve the overall bioassimilation in the digestive tract but are still not proven to increase ... developed symbiotic relationships with cellulase-producing bacteria (see termites and metamonads.) This allows termites to use ...
Henrissat B, Tomme P, Claeyssens M, Mornon JP, Lemesle L (1989). "Cellulase families revealed by hydrophobic cluster analysis ... These enzymes were formerly known as cellulase family D. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995 ...
One of these families is known as the cellulase family A or as the glycosyl hydrolases family 5. One of the conserved regions ... Haiech J, Chippaux M, Barras F, Py B, Bortoli-German I (1991). "Cellulase EGZ of Erwinia chrysanthemi: structural organization ... Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence ... Badieyan, S; Bevan DR; Zhang C (January 2012). "Study and design of stability in GH5 cellulases". Biotechnology and ...
... "was drawn into the cellulase area by some comments I was asked to present at a symposium dealing with cellulase of the fungus ... the inhibitor of cellulase action." An important theory proposed by Demain, which he showed to be valid, was that antibiotics ... "the first demonstration of a true cellulase produced by a bacterium." This enzyme later proved to be "useful in the conversion ... cellulases and ethanologenic clostridia; vitamin B12; amino acids, organic acids and polymers; the immunosuppressant, rapamycin ...
Gilbert, H. J.; Hazlewood, G. P. (1993). "Bacterial cellulases and xylanases". Journal of General Microbiology. 139 (2): 187- ...
Szakács, György; Réczey, Kati; Hernádi, Pál; Dobozi, Mária (1981). "Penicillium verruculosum WA 30 a new source of cellulase". ... "Cellulases of Penicillium verruculosum". Biotechnology Journal. 5 (8): 871-80. doi:10.1002/biot.201000050. PMID 20540109. Shah ... "Cellulases of Penicillium verruculosum". Biotechnology Journal. 5 (8): 871. doi:10.1002/biot.201000050. PMID 20540109. Wigney, ... "Enzymatic hydrolysis of pre-treated lignocellulose with Penicillium verruculosum cellulases". Journal of Molecular Catalysis B ...
Their plan was to see if they could stick the four enzymes used in washing powder - á-amylase, cellulase, protease and lipase- ... A biological detergent can contain a cocktail of enzymes: α-amylase - to break down starch based stains; cellulase - to clean ...
Cellulase was already used in the paper pulp, food processing industry and currently in the fermentation of biomass for biofuel ... "Cellulase enzymes won't leave your laundry washed up." Bobbin Dec. 1993: 62+. General OneFile. Web. 9 Aug. 2013 Men's fashion ... Cellulase is produced primarily by fungi, bacteria and protozoan that catalyze the hydrolysis of cellulose. Since the enzyme ... Claude Blankiet with American Garment Finishers from Texas promoted the use of cellulase enzymes in the finishing industry. ...
nov., a cellulase-free endo-xylanase-producing streptomycete". International Journal of Systematic and Evolutionary ... nov., a cellulase-free endo-xylanase-producing streptomycete". International Journal of Systematic and Evolutionary ...
Gupta, D. P.; Heale, J. B. (1970). "Induction of Cellulase (Cx) in Verticillium albo-atrum". Journal of General Microbiology. ...
This is consistent with cellulase not being toxic to yeast and no mention was made of any toxic effect of cellulases on the ... Enzymes are known to be highly specific and in general cellulases have not been found to be active on chitin. To quote one ... This again suggests cellulases do not in general contain significant chitinase activity. Similarly, although many internet ... Indeed, scientists have even modified yeast cells so they can make their own cellulases. This allows the modified yeast to ...
"Enzyme exhibiting cellulase activity - US Patent 5916796 Full Text". Patentstorm.us. Archived from the original on 11 October ... Hansen has filed numerous patent applications in the fields of genetics of bacteria, cellulases and other enzymes, osteoporosis ...
Kasulke, U.; Philipp, B.; Polter, E. (1987). "Zur Wechselwirkung zwischen einer Penicillium-Cellulase und wasserlöslichen ...
1967) Senescence, abscission and cellulase activity in Phaseolus vulgaris. Nature 214: 1086-1088 (abstract) Ellis PE, Carlisle ...
... cellulase A, cellulosin AP, alkali cellulase, cellulase A 3, 9.5 cellulase, and pancellase SS. Enzymes that cleave lignin have ... Cellulose 1,4-beta-cellobiosidase, an efficient cellulase Cellulase unit, a unit for quantifying cellulase activity PDB: 1NLR​ ... Cellulase action is considered to be synergistic as all three classes of cellulase can yield much more sugar than the addition ... Cellulases are produced by a few types of animals, such as some termites. Several different kinds of cellulases are known, ...
Dissolve 1 g cellulase in 99 ml sterile distilled water. Filter sterilize through a 0.45 µm filter. Cellulase solution may be ...
A cellulase unit (CU) is a unit of measurement for the enzyme cellulase. One cellulase unit is that activity that will produce ... Cellulases enzymatically split cellulose into a variety of hexoses, this is called hydrolysis. Eagle Pack Archived February 21 ...
Cellulases (Endoglucanases) EC:3.2.1.4 catalyse the endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose. This is the ... N-terminal ig-like domain of cellulase, enzymes containing this domain belong to family 9 of the glycoside hydrolases (GH9). ...
Family 48 cellulases are a prominent component of many bacterial cellulase systems and, due to their ubiquity, are thought to ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ... 1993) Cellulase Ss (CelS) is synonymous with the major cellobiohydrolase (subunit S8) from the cellulosome of Clostridium ...
... and coconut cake stimulates the production of cellulase. Among bacteria, Pseudomonas fluorescens is the best cellulase producer ... Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and ... Optimization of Cellulase Production from Bacteria Isolated from Soil. Sonia Sethi, Aparna Datta, B. Lal Gupta, and Saksham ... The optimum conditions found for cellulase production were 40°C at pH 10 with glucose as carbon source and ammonium sulphate as ...
Cellulase is used in many places, primarily the agricultural sector. Its often used as a food additive and to produce juices ... The primary role of the enzyme cellulase is to split cellulose.. ... Cellulase is found in all plants and is quite abundant. In fact ... The primary role of the enzyme cellulase is to split cellulose.. Cellulase is used in many places, primarily the agricultural ... Cellulase naturally occurs in plant tissues; there, it acts as a reinforcing material. It is thick and viscous in its physical ...
NOTERelationship Between Cellulase Treatment and Direct Dye Dyeing for Cotton INTRODUCTION ... ... treated with cellulase for 24 h and then the WL was ex- could be attacked by cellulase with more difficulty. amined. The dyeing ... with Cellulase by cellulase-catalyzed hydrolytic cleavage. If the cleavage causes a change in -Ape, -Apo would monotonously WL ... rics caused by cellulase treatment. Voand V, are effective volume terms of dyeing for untreated and cellulase-treated We would ...
Model of cellulase induction mediated by CLR-1 and CLR-2 in N. crassa. When de-repressed, genes encoding cellulases, β- ... Despite the complete loss of induced cellulase gene expression and cellulase secretion in Δclr-1 and Δclr-2 strains, growth and ... Fungal cellulase gene expression and secretion are tightly controlled at the transcriptional level (4⇓-6). The most extensive ... From these data we conclude that clrA has a less important role in cellulase induction in A. nidulans compared with clr-1 in N ...
A Moderate Drug Interaction exists between cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and Zymine HC. View ... Applies to: cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and cellulase / hyoscyamine / pancrelipase / ... Drug Interactions between cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and Zymine HC. This report displays the ... Applies to: cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and Zymine HC (hydrocodone / pseudoephedrine / ...
The maximum cellulase activity was observed at 4.59 g/L of KH2PO4. Figures 6 and 7 show the dependency of cellulase activity on ... were found to be significant for the production of cellulase. The coefficient of determination for cellulase activity was ... The maximum cellulase activity (8.61 IU/mL) was obtained from experiments which was very close to the cellulase production ( ... The effect of carbon source (avicel) on cellulase production has been shown in Figure 2 in which the cellulase activity is ...
A preferred cellulase has a calculated molecular weight of approximately 63 kD, a calculated isoelectric point of about 5 and a ... The present invention provides a novel cellulase composition obtainable from Bacillus sp. CBS 669.93. ... Thus cellulase with an identical amino acid sequence to the 63 kD cellulase of the invention derived from a different Bacillus ... A cellulase is "obtainable from" Bacillus 668.93 if such cellulase has an amino acid sequence which corresponds to the amino ...
A Moderate Drug Interaction exists between cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and Ricobid-D. View ... Drug interactions between cellulase / hyoscyamine / pancrelipase / phenyltoloxamine and Ricobid-D. Edit this list ...
... dispersed in water by a commercial crude cellulase was studied. Products crude cellulase-treated for 0-7 days were separated ... Biodegradation Cellulase Hydrolase Nanofibril TEMPO-oxidized cellulose This is a preview of subscription content, log in to ... newly exposed on the microfibril surfaces were rapidly hydrolyzed by cellulases predominantly present in the crude cellulase to ... but TOCN having free carboxyl groups had clearly low biodegradability by the crude cellulase. Thus, biodegradation behavior may ...
Accepted Manuscript: Interrelationships between cellulase activity and cellulose particle morphology. Title: Interrelationships ... plays a major role in the ubiquitous rate loss observed for cellulases.« less ...
Cellulase, thermostable from Clostridium thermocellum recombinant, expressed in E. coli, ≥90% (SDS-PAGE) * pricing ... Cellulase, thermostable from Dictyoglomus turgidum recombinant, expressed in E. coli, ≥90% (SDS-PAGE) * pricing ...
Find user ratings and reviews for amylase-lipase-protease-cellulase oral on WebMD including side effects and drug interactions ... Read user comments about the side effects, benefits, and effectiveness of amylase-lipase-protease-cellulase oral. ...
... cellulase / hyoscyamine / lipase / phenyltoloxamine / protease and Nytol QuickCaps. View detailed information regarding this ... Drug interactions between amylase / cellulase / hyoscyamine / lipase / phenyltoloxamine / protease and Nytol QuickCaps. Edit ... Applies to: Nytol QuickCaps (diphenhydramine) and amylase / cellulase / hyoscyamine / lipase / phenyltoloxamine / protease ... Applies to: Nytol QuickCaps (diphenhydramine) and amylase / cellulase / hyoscyamine / lipase / phenyltoloxamine / protease ...
Cellulase Y-C enzyme retains very high filter paper decomposing activity and showed an appreciable amount of hemicellulase. ... Cellulase Y-C (From Trichoderma Viride). Synonyms 1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase ... Cellulase is used for protoplast preparations. This enzyme shows a very high filter paper decomposing activity, as well as, ... Cellulase is used for protoplast preparations. This enzyme shows a very high filter paper decomposing activity, as well as, ...
CELLULASE FROM TRICHODERMA LONGIBRACHIATUM First draft prepared by Dr D.L. Grant and Dr E. Vasavour Health and Welfare Canada ... Some additional studies on cellulase from T. longibrachiatum (reesei) have been submitted and are presented in this monograph ... OSBORNE, B.E., RUSHTON, A.K.A. & DENT, N.J. (19777) Cellulase SP-122, toxicity study in beagle dogs (oral administration for 13 ... Cellulase enzyme preparation derived from Trichoderma longibrachiatum. Unpublished report from Toxicol Laboratories Ltd., ...
Cellulase (Onozuka R-10), Cellulase, SIGMA Cellulase from Aspergillus niger powder, ,=0.3 units/mg solid, SIGMA Cellulase from ... Cellulase RS RPI (Research Products International). From mutant Trichoderma viride derived from the parent strain for Cellulase ... Synonym(s): Cellulase Storage: −20°C Included Fees: C957K78 has a Dry/Wet Ice Fee included in the price. To see the value of ... SIGMA Cellulase from Aspergillus niger powder, >=0.3 units/mg solid MilliporeSigma. Synonym(s): 1,4-(1,3:1,4)-β-D-Glucan 4- ...
Cellulase production medium for the growth and cellulase production had the following composition: KH2PO4 2 g L−1; (NH4)2SO4 ... Cellulase Activity. Cellulase, as filter paper activity (FPA), was determined according to the 96 μL microplate-based (MPB) ... Cellulase Production in Liquid Culture. In order to determine the cellulase production capacity of the 11 selected strains, ... It was found that pH did not affect cellulase activity corroborating that all strains are true cellulase producers and that ...
A was found to produce cellulases and polyphenol oxidase. In time course analyses of cellulase production in batch cultures, ... Analysis of cellulase and polyphenol oxidase production by southern pine beetle associated fungi. Symbiosis, Vol. 49: 37-42. ... Cellulase and polyphenol oxidase production were analyzed for three beetle associated fungi. Only the mutualistic symbiont ... Ceratocystiopsis, Entomocorticium, Ophiostoma, cellulase, tyrosinase/catechol oxidase. Related Search. *From Attack to ...
... Karin Vega, Gretty K. Villena, ... LM-HP37 were the best producers of FP cellulase (,3 U mL−1) with higher specific productivities (,30 U g−1 h−1). Three strains ... Alkaline cellulase producing fungi were isolated from soils of an undisturbed rain forest of Peru. The soil dilution plate ... Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry. ...
Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for ... Cellobiase, cellulase β-glucosidase industrial enzyme preparations. This is a preview of subscription content, log in to check ... Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger. ... Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for ...
  • Other industrial uses include using the various cellulases for staining fabrics in the textile industry, increasing digestibility of poultry feed, and potentially in the generation of biofuels. (wikipedia.org)
  • Purification and characterization of a cellulase (CMCase) from a newly isolated thermophilic aerobic bacterium Caldibacillus cellulovorans gen. nov., sp. (springer.com)
  • Shu G-W, He Y-X, Lei N, Cao J-L, Chen H, Chen L. Cellulase-Assisted Extraction of Polysaccharides from White Hyacinth Bean: Characterization of Antioxidant Activity and Promotion for Probiotics Proliferation. (mdpi.com)
  • The characterization of cellulase produced from MSL2 strain was described here. (ejbiotechnology.info)
  • Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance", Kern et al, PNAS June 3, 2013 R. J. Menzies. (wikipedia.org)
  • Banana peels are also used for water purification, to produce ethanol, cellulase, laccase, as fertilizer and in composting. (wikipedia.org)
  • Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. (patents.com)
  • Project researchers will focus on the prevalent biological route to cellulose conversion: enzymatic hydrolysis by cellulases. (anl.gov)
  • We present a coarse-grained stochastic model for capturing the key events associated with the enzymatic degradation of cellulose at the mesoscopic level.Importantly, it captures the endo-exo synergism of cellulase enzyme cocktails.This model constitutes a critical step towards testing hypotheses and understanding approaches for maximizing synergy and substrate properties with a goal of cost effective enzymatic hydrolysis. (nih.gov)
  • Avicelase has almost exclusively exo-cellulase activity, since avicel is a highly micro-crystalline substrate. (wikipedia.org)
  • One cellulase unit is that activity that will produce a relative fluidity change of one in 5 minutes in a defined carboxymethylcellulose substrate under the conditions of an assay (50 deg Celsius, pH 4.5). (wikipedia.org)
  • Carboxymethyl- treatment on the dyeability of the materials must be stud- cellulase activity was 233,000 units/g as determined by ied. (scribd.com)
  • 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment. (hindawi.com)
  • Cellulase RS contains a high activity of decomposing natural celluloses. (thomassci.com)
  • Cellulases have recently been found with optimal activity at neutral to alkaline pH, and these are gradually being incorporated into fiber treatment processes, especially in the polishing and washing of denim processing because the alkaline or neutral pH reduces textile backstaining [ 9 - 11 ]. (hindawi.com)
  • A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. (springer.com)
  • Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. (scielo.br)
  • Super thermostable cellulase maintains its activity at temperatures up to 90°C, which is consistent with our data illustrating that the thermal denaturation of the protein can only be seen for temperatures above 100°C. (jascoinc.com)
  • Callus tissue, in which no vascular differentiation occurs, contains only low cellulase activity. (sheldrake.org)
  • Phloem tissue also contains a soluble, cytoplasmic cellulase of high activity at pH 7.1 and xylem tissue contains cytoplasmic cellulase with an optimum at pH 6.5. (sheldrake.org)
  • Low cellulase activity with a pH optimum similar to that of xylem homogenates was found in xylem sap. (sheldrake.org)
  • Cellulase activity in abscission zones increases greatly just before leaf abscission. (sheldrake.org)
  • It is a compound cellulase preparation with some activity of hemi- cellulase . (chinacsw.com)
  • 24. The isolated chimeric fungal Cel7A polypeptide of claim 21, wherein the chimeric fungal Cel7A polypeptide has a cellulase activity at least 1.5-fold greater than the wild-type Cel7A polypeptide. (patents.com)
  • Importantly, misexpression of clr-2 under noninducing conditions was sufficient to drive cellulase gene expression, secretion, and activity in N. crassa, to a level comparable to wild type exposed to Avicel. (biomedsearch.com)
  • However, misexpression of clrB in A. nidulans was not sufficient to drive cellulase gene expression under noninducing conditions, although an increase in cellulase activity was observed under crystalline cellulose conditions. (biomedsearch.com)
  • The pH and temperature profile showed optimal activity at pH 3.0 and 60 ∘ C . As for thermostability, the cellulase was most tolerant at 60 ∘ C , retaining more than 59.6% of maximal activity even after 4 hours of incubation. (hindawi.com)
  • On glucose, Δpgi1 and cre1+Δpgi1 mutants showed higher cellulase activity than Rut-C30 and QM6a, respectively. (unboundmedicine.com)
  • The lower cellulase activity of these mutants in media with lactose could be attributed to a reduced ability to hydrolyse this sugar but not to an effect on the expression of xyr1. (unboundmedicine.com)
  • Hankin L, Anagnostakis S (1977) Solid media containing carboxy methyl cellulose to detect CM cellulase activity of microorganisms. (springer.com)
  • In the presence of the IL, 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) concentration of 1 M, the cellulase activity retained 77.7% of non-IL condition. (ejbiotechnology.info)
  • However, this cellulase retained its activity more than 90% at 55°C, and pH 4.0. (ejbiotechnology.info)
  • Cellulase-lignophenol complex was available as an immobilized cellulase with 20-60% of enzymatic activity of native cellulase with wider optimum pH range. (nii.ac.jp)
  • The concentration (or actually dilution) of enzyme preparation required to effect this level of depolymerization is converted, through a somewhat indirect procedure, to the cellulase activity in filter paper units (FPU) per milliliter. (asmscience.org)
  • Like most Chaetomium species, C. globosum decomposes plant cells using hyphal cellulase activity. (wikipedia.org)
  • The cellulase activity of C. globosum functions best at temperatures ranging from 25-32 degrees Celsius and is stimulated by nitrogen and biotin. (wikipedia.org)
  • This again suggests cellulases do not in general contain significant chitinase activity. (wikipedia.org)
  • The larvae feed on decaying wood, absorbing substances liberated by microbial activity-they lack their own cellulase or endosymbionts. (wikipedia.org)
  • "Determination of Product Inhibition of CBH1, CBH2, and EG1 using a Novel Cellulase Activity Assay" Zhu, J. Y. et. (wikipedia.org)
  • It was essential to be able to measure cellulase activity, and Mandels developed an assay to predict the quantity of enzyme required. (wikipedia.org)
  • Lin YS, Yang CC, Hsu CC, Hsu JT, Wu SC, Lin CJ, Cheng WT (2015) "Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes. (cosmobio.co.jp)
  • Gram's iodine formed a bluish-black complex with cellulose but not with hydrolyzed cellulose, giving a sharp and distinct zone around the cellulase-producing microbial colonies within 3 to 5 minutes. (springer.com)
  • The genetic improvement study will make the isolate a good source of cellulase. (nepjol.info)
  • It is a source of cellulase. (wikipedia.org)
  • This application note describes the thermal denaturation analysis of super thermostable cellulase using the TC-700PC with Denaturation Analysis program. (jascoinc.com)
  • 1.2 mg/mL of super thermostable cellulase in 20 mM Tris-HCl buffer (pH 8.0) was diluted with distilled water to the desired concentrations and measured using the J-1500 and TC-700PC. (jascoinc.com)
  • The CD spectra of 0.01 mg/mL super thermostable cellulase was measured at 45, 80, 100 and 120°C and is shown in Figure 1. (jascoinc.com)
  • Far-UV (195-260 nm) CD spectra of 0.01 mg/mL of super thermostable cellulase at 45°C (red), 80°C (blue), 100°C (yellow) and 120°C (green). (jascoinc.com)
  • CD measurements were also obtained at 220 nm to probe the thermal denaturation of super thermostable cellulase. (jascoinc.com)
  • Consistent with Figure 1, there is a sharp decrease in the CD value at temperatures over 100°C, indicating the loss of secondary structure and denaturation of the protein above 100°C. The melting temperature (Tm) of super thermostable cellulase was calculated by the Denaturation Analysis software and found to be 106.3°C. (jascoinc.com)
  • Lamppa, Gayle 2004-10-07 00:00:00 A bacterial thermostable cellulase, the endo-1,4-β-D-glucanase E1 from Acidothermus cellulolyticus, was imported into chloroplasts, and an active enzyme was recovered both in vitro and in vivo. (deepdyve.com)
  • Other genus-specific transcriptional activators (such as ACE2 in H. jecorina ) appear to have some specificity for particular inducers, but deletion strains for these transcription factors still induce significant cellulase secretion ( 15 ). (pnas.org)
  • All 11 strains produced cellulases in liquid culture with activities at alkaline pH values without an apparent decrease of them indicating that they are true alkaline cellulase producers. (hindawi.com)
  • Such HAX1 overexpression on the one hand was suitable for regaining the function in hax1 disruption strains, and on the other hand resulted in notably higher cellulase activities in QM6a, especially by the expression of longer HAX1 versions. (springer.com)
  • strains were selected for their capacity to produce alkaline cellulases. (biomedsearch.com)
  • Culture supernatants of these strains showed optimal cellulase activities between pH 8 and 9 and they were stable from pH 6 to pH 12. (biomedsearch.com)
  • These "improvement programs" originally consisted of classical (ionising-radiation-based and chemical-based) mutagenesis, which led to strains capable of producing 20 times as much cellulase as the QM6a isolate. (wikipedia.org)
  • Some of the highest performing industrial strains produce up to 100 grams of cellulases per litre, more than 3 times as much as the RUT-C30 strain (which itself produces twice as much as the parent strain NG14 from which it was derived). (wikipedia.org)
  • The results of this study should provide a basis for the development of a more effective fungal strain capable of producing cellulase cocktails with enhanced hydrolytic performance against lignocellulosic materials. (springer.com)
  • Disruption of CipA by random integration of insertion elements has been reported in C. thermocellum ( 16 ), and targeted gene inactivation mediated by group II introns has been used to evaluate family 9 cellulase function in the mesophile C. phytofermentans ( 11 ). (pnas.org)
  • Fungal cellulase gene expression and secretion are tightly controlled at the transcriptional level ( 4 ⇓ - 6 ). (pnas.org)
  • GMLF7, and cellulase encoding bifunctional gene celA-5 from Orpinomyces sp. (scielo.br)
  • Regulated control of cellulase gene expression across divergent fungal species would allow for high productivity of varied enzyme mixtures under a range of growth conditions and media compositions. (biomedsearch.com)
  • Moreover, in contrast to the previously mentioned fact that yeast bioengineered to make cellulases remain healthy, when a chitinase gene was bioengineered into the same yeast strain it resulted in complete growth inhibition. (wikipedia.org)
  • Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry. (hindawi.com)
  • The quantitative description of cellulose degradation is calculated on a spatial model by including free and bound states of both endo- and exo-cellulases with explicit reactive surface terms (e.g., hydrogen bond breaking, covalent bond cleavages) and corresponding reaction rates. (nih.gov)
  • It appears likely that P. placenta employs a combination of oxidative mechanisms and endo-acting cellulases to degrade cellulose efficiently in the absence of a significant processive component. (usda.gov)