Local surroundings with which cells interact by processing various chemical and physical signals, and by contributing their own effects to this environment.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
Methods for maintaining or growing CELLS in vitro.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Theory and development of COMPUTER SYSTEMS which perform tasks that normally require human intelligence. Such tasks may include speech recognition, LEARNING; VISUAL PERCEPTION; MATHEMATICAL COMPUTING; reasoning, PROBLEM SOLVING, DECISION-MAKING, and translation of language.
A cell line derived from cultured tumor cells.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Any of several processes in which undesirable impurities in water are removed or neutralized; for example, chlorination, filtration, primary treatment, ion exchange, and distillation. It includes treatment of WASTE WATER to provide potable and hygienic water in a controlled or closed environment as well as provision of public drinking water supplies.
The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
Preparations made from animal tissues or organs (ANIMAL STRUCTURES). They usually contain many components, any one of which may be pharmacologically or physiologically active. Tissue extracts may contain specific, but uncharacterized factors or proteins with specific actions.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Aggressive T-Cell malignancy with adult onset, caused by HUMAN T-LYMPHOTROPIC VIRUS 1. It is endemic in Japan, the Caribbean basin, Southeastern United States, Hawaii, and parts of Central and South America and sub-Saharan Africa.
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
A malignant disease of the T-LYMPHOCYTES in the bone marrow, thymus, and/or blood.
Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Heat production, or its measurement, of an organism at the lowest level of cell chemistry in an inactive, awake, fasting state. It may be determined directly by means of a calorimeter or indirectly by calculating the heat production from an analysis of the end products of oxidation within the organism or from the amount of oxygen utilized.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.
Degenerative changes in the RETINA usually of older adults which results in a loss of vision in the center of the visual field (the MACULA LUTEA) because of damage to the retina. It occurs in dry and wet forms.

Screening the cellular microenvironment: a role for microfluidics. (1/313)

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Murine amniotic fluid stem cells contribute mesenchymal but not epithelial components to reconstituted mammary ducts. (2/313)

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Salivary glands of primary Sjogren's syndrome patients express factors vital for plasma cell survival. (3/313)

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Evolving homeostatic tissue using genetic algorithms. (4/313)

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Mathematical descriptions of biochemical networks: stability, stochasticity, evolution. (5/313)

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More than a feeling: discovering, understanding, and influencing mechanosensing pathways. (6/313)

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Toll-like receptors are key players in neurodegeneration. (7/313)

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Nattectin a fish C-type lectin drives Th1 responses in vivo: licenses macrophages to differentiate into cells exhibiting typical DC function. (8/313)

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TY - JOUR. T1 - Switchable substrates for analyzing and engineering cellular functions. AU - Nakanishi, Jun. PY - 2014/2/1. Y1 - 2014/2/1. N2 - Cellular activity is highly dependent on the extracellular environment, which is composed of surrounding cells and extracellular matrices. This focus review summarizes recent advances in chemically and physically engineered switchable substrates designed to control such cellular microenvironments by application of an external stimulus. Special attention is given to their molecular design, switching strategies, and representative examples for bioanalytical and biomedical applications.. AB - Cellular activity is highly dependent on the extracellular environment, which is composed of surrounding cells and extracellular matrices. This focus review summarizes recent advances in chemically and physically engineered switchable substrates designed to control such cellular microenvironments by application of an external stimulus. Special attention is given to ...
The colon cancer stem cell microenvironment holds keys to future cancer therapy.s profile, publications, research topics, and co-authors
Abstract While most of the in vitro cultures are carried out on bi-dimensional (2D) substrates, most of the in vivo extracellular matrices are threedimensional (3D). Consequently cells behave differently on 2D substrates as a way to self-adaptation to a non-physiological environment. This fact has encouraged the development of more relevant culture conditions seeking to provide more representative models for biomedicine (e.g. cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. Different 3D culture systems have been established though their variability and complexity hinder their standardisation in common cell culture procedures. So, this thesis deals with the dimensionality issue in cell/material interactions and introduces sandwich-like microenvironments as a versatile tool to study cell behaviour. Cells cultured within this system use both dorsal and ventral receptors to adhere and spread, undergoing important changes with ...
The objective of the presented research is to examine the relationship between the cellular microenvironment and biochemical response of metastatic cells. Clinically recognized as a trait of cancer progression, the cellular microenvironment can have variable and distinct mechanical properties that are processed via cellular mechanosensing, resulting in a cellular biochemical response. A range of studies investigating the interactions between the cellular micromechanical environment and the cells molecular response during disease progression have been made, yet remain absent of quantitative characterization of many of these coordinated responses. The fundamental inquiry that drives the following research attempts to elucidate how a cell perceives the physical microenvironment and converts that signal to a biochemical response. With the goal of providing insight to such responses, the presented research seeks to elucidate the following questions: (1) What are the integrated effects of ECM ...
Solid tumors are composed of heterogeneous cell populations that interact in complex networks. As is the case in developing organs, tumor cells interact with and in turn are regulated by these components in the microenvironment. Metastatic tumor cells also recreate complex cellular microenvironments at metastatic sites. More than 120 years ago, Paget proposed the seed and soil hypothesis of tumor metastasis (40). Reframed in a modern context, the seeds are the CSCs and the soil is the rich microenvironment, which is composed of diverse cell types that interact with tumor cells via cytokine networks. These networks regulate CSCs and their progeny, which form the tumor bulk. Elucidation of these pathways may provide new targets for therapeutic development. Examples of such pathways include the cytokines IL-6 and IL-8 and their receptors IL-6R and CXCR1. Blockade of these cytokine pathways reduced breast CSCs in preclinical models (33). Clinical trials using IL-6-blocking antibodies have been ...
Several reports have pointed out that the ECM inhibits regeneration and suppresses plasticity in the adult CNS (Berardi et al., 2004; Fitch and Silver, 2008; Gervasi et al., 2008). By comparison, the role of the ECM in the stem and precursor cell microenvironment remains obscure, although the cellular microenvironment is crucial for stem cell maintenance and self-renewal (Scadden, 2006). Focusing on OPCs as a model, we reveal an inhibitory function of the ECM glycoprotein Tnc on differentiation and disentangle molecular details of ECM pathways designed to suppress the differentiation of a committed neural precursor cell. We show that Tnc suppresses the expression of MBP in oligodendrocytes both in vitro and in vivo during postnatal forebrain development, confirming its biological significance. The underlying signaling cascade requires the cell surface receptor Cntn1 and the associated Fyn kinase, and involves the regulation of the signaling adaptor and splice regulator Sam68. We further document ...
TY - JOUR. T1 - Association of RB/p16-pathway perturbations with DCIS recurrence. T2 - Dependence on tumor versus tissue microenvironment. AU - Witkiewicz, Agnieszka K.. AU - Rivadeneira, Dayana B.. AU - Ertel, Adam. AU - Kline, Jessica. AU - Hyslop, Terry. AU - Schwartz, Gordon F.. AU - Fortina, Paolo. AU - Knudsen, Erik S.. PY - 2011/9/1. Y1 - 2011/9/1. N2 - The prevalence of ductal carcinoma in situ (DCIS) diagnoses has significantly increased as a result of active radiographic screening. Surgical resection and hormone and radiation therapies are effective treatments, but not all DCIS will progress to invasive breast cancer. Therefore, markers are needed to define tumors at low risk of recurrence and progression that can be treated by surgery alone rather than by adjuvant therapies. Initial analyses indicate that retinoblastoma (RB)-pathway perturbations occur at high frequency in DCIS and mirror the molecular alterations observed in invasive breast cancer. Particularly, the elevated ...
Participate in the open-access Research topic entitled The Adipose Tissue Microenvironment in Cancer: Molecular Mechanisms and Targets for Treatment
Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression ...
Thanks to the co-financing of S. Spicuglia (U1090) and a mutual request of the units to the region (U1067-U1090-U1097), the common cell culture platform of the building has recently acquired: a sorter BD FACSMelody: 3 lasers, 9 colors and single-cell module a BD LSRFortessa X20 cell analyser : 3 lasers, 14 colors
Today is 04/02/10 and Id mentioned that I would follow up after i perm my hair post BKT treatment. So to recap, Id got the BKT at a high-end dept.
J. S. Miller and West, J. L., Biomimetic hydrogels to support and guide tissue formation, in Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications, A. Khademhosseini, Borenstein, J., Toner, M., and Takayama, S. 2008, pp. 101-120. ...
The magic to killing cancer cells might not be in the bullet, but in the gunpowder that accompanies it, according to researchers at the University of Pittsburgh Cancer Institute (UPCI), partner with UPMC CancerCenter.
Living systems are made of complex architectural organization of various cell types in defined microenvironments. The intricate interactions between different cell types control the specific functions of the associated tissues, such as the functions of native liver and cardiac tissues, metastasis and invasion of tumors, and embryonic development.
Elasticity of cellular microenvironments strongly influences cell motility, phagocytosis, growth and differentiation. Currently, the relationship between the cell behaviour and matrix stiffness is being studied for cells seeded on planar substrates, however in three-dimensional (3D) microenvironments cells may experience mechanical signalling that is distinct from that on a two-dimensional matrix. We report a microfluidic approach for high-throughput generation of 3D microenvironments with different elasticity for studies of cell fate. The generation of agarose microgels with different elastic moduli was achieved by (i) introducing into a microfluidic droplet generator two streams of agarose solutions, one with a high concentration of agarose and the other one with a low concentration of agarose, at varying relative volumetric flow rate ratios of the two streams, and (ii) on-chip gelation of the precursor droplets. At 37 °C, the method enabled a ~35-fold variation of the shear elastic modulus ...
Injury-induced stem-cell niche are defined as cellular microenvironments that are generated during tissue injury, these environments are triggered by injury and the local responses of support cells, and enable the possibility of repair by endogenous or transplanted neural stem cells. These environments have been demonstrated in several injury models, most notable in the CNS. The term was coined by Jaime Imitola and Evan Y. Snyder when they demonstrated that astrocytes and endothelial cells during stroke are able to create a permissive environment for neural regeneration,[1] that is most striking for exogenous transplanted neural stem cells. Previous work by the Snyder Laboratory have shown that the interactions between NSCs and local cells is reciprocal, underlying a bystander beneficial effect of neural stem cells without neural differentiation, once thought to be the only mechanism for therapeutical benefit of stem cells in CNS injury.[2]. More recently these findings have been reproduced and ...
It isnt about who discovers the remedy first. Its about getting a cure - fast... BCBC awards $5 million for cell therapy analysis in type 1 diabetes Maike Sander, MD, associate professor of pediatrics and cellular & molecular medicine at the University of California, NORTH PARK School of Medication has been awarded nearly $5 million by the Beta Cell Biology Consortium to business lead an interdisciplinary group in cell therapy analysis for type 1 diabetes. Sander will business lead a united group of domestic and worldwide collaborators, with the purpose of generating substitute insulin-making beta cells from patient-derived induced pluripotent stem cells. The $4,950,000 grant is distributed to Karl Willert, PhD, director of the UCSD Human being Stem Cell Core Service, wholl apply a screening system for cellular microenvironments to beta cell differentiation Additional individuals consist of labs at the University of Pennsylvania and in Barcelona, Spain, in addition to a San Diego-structured ...
2509 Nitric Oxide (NO•), an important signaling molecule and a component of inflammatory response, is also involved in tumorigenesis. However, the quantity of NO• and the nature of the cellular microenvironment influences the role of NO• in tumor development. We used a genetic strategy to test the hypothesis that an inflammatory microenvironment with an enhanced level of NO• accelerates spontaneous tumor development. C. parvum-induced inflammation and increased nitric oxide synthase-2 (NOS2) expression which coincided with accelerated spontaneous tumor development, mostly lymphomas, in p53-/-NOS2+/+ C57BL6 mice as compared to the controls (p=0.001). However, p53-/-NOS2-/- mice did not show any difference in tumor latency between C. parvum-treated and control groups. C. parvum-treated p53-/-NOS2+/+ mice showed that tumor development was preceded by a higher expression of NOS2 and phosphorylated Akt-Ser 473 (pAkt-Ser473) in spleen, increased cell proliferation measured by Ki-67 ...
Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.. ...
Protein delivery is often used in tissue engineering applications to control differentiation processes, but is limited by protein instability and cost. An alternative approach is to control the cellular microenvironment through biomaterial-mediated sequestration of cell-secreted proteins important to differentiation. Thus, we utilized heparin-based microparticles to modulate cellular differentiation via protein sequestration in an in vitro model system of endochondral ossification. Heparin and poly(ethylene-glycol) (PEG; a low-binding material control)-based microparticles were incorporated into ATDC5 cell spheroids or incubated with ATDC5 cells in transwell culture. Reduced differentiation was observed in the heparin microparticle group as compared to PEG and no microparticle-containing groups. To determine if observed changes were due to sequestration of cell-secreted protein, the proteins sequestered by heparin microparticles were analyzed using SDS-PAGE and mass spectrometry. It was found ...
Clone DB105 recognizes the CD44 antigen. CD44 is a marker for many types of cancer stem cells (CSCs), including breast CSCs that possess higher tumorigenicity and metastatic potential, colorectal, pancreatic, and prostate CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, CD44 plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. - Österreich
However, when cancer cells secrete fresh material into the ECM, it disrupts the chemical and physical composition of the native ECM surrounding healthy cells, degrading the local environment.. Therefore, understanding how the cellular microenvironment is altered due to cancer cells and measuring these changes quantitatively could be vital in understanding the progression of cancer, IISc noted.. In the current study, the researchers discovered that as the nanomotors approached the cancer cell membrane, they stuck to the matrix more strongly than they would to normal cells.. To measure how strongly the nanomotors bound to the matrix, the team calculated the magnetic field strength required to overcome the adhesive force, and move forward, it was stated.. This means that the cancer cells are doing something.. So, we did some measurements and discovered that it [the adhesive force] depended on the type of cells, the strength of interaction and also which side of the cell the nanomotor ...
Organs-on-Chip - Mimicking the in-vivo cellular microenvironment During more than 100 years cell biologists have been culturing cells on flat and hard substrates, called Petri dishes. Plastics replaced glass, but the plates Julius Richard Petri invented in 1887 havent changed and are still widely used today. Although an instrumental tool for life-saving medical breakthroughs, like Sir Alexander Flemings discovery of penicillin, the in-vitro environment provided by Petri dishes widely differs from the native environments the cells are accustomed to in the body. As a result, they often lose their original functions and are no longer suitable to be used as models to test for instance drug candidates. The pharmaceutical industry increasingly recognized this fact and turns more and more to new in-vitro models, such as organs-on-chip, that more accurately represent the milieu in the human body. Organs-on-chip are advanced in-vitro models that mimic the smallest functional unit of an organ. These ...
Scientists describe the optimization of the fabrication process for a novel, detailed, 3D cell culture platform based on direct laser written tubular microtowers and human neuronal cells.
Most cancer deaths are due to spreading of the primary tumor to one or several secondary sites, in a process called metastasis. These metastases are often more difficult to treat than the primary tumor and their presence marks severe progression of the disease. Tumor cell metastasis involves cell migration through heterogenous microenvironments in tissues, along anatomical features such as blood vessels and nerves, and into and out of vasculature. Mounting evidence has accumulated, demonstrating that metastatic tumor cells can migrate via several modes or mechanisms of migration, and can switch between these modes depending on the specific features of the microenvironment. Our research in this area is focused on understanding how distinct physical and biochemical cues from the tumor cells microenvironment affect the phenotype, biological signaling, mechanical properties, and interactions with other cells during metastasis. We are interested in metastasis to the brain, which is especially ...
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
Background:M. tuberculosis infection either induces or inhibits host cell death, depending on the bacterial strain and the cell microenvironment. There is evidence suggesting a role for mitochondria in...
Date: September 24, 2008 at 11:44 am PST. In Reply to: BKT EXPERTS PLEASE HELP! posted by dee on August 10, 2008 at 2:48 pm:. Hey I just read your post. I do both Japanese & Brazilian Straightening. I like both a lot. But there are a few things to take into consideration before making your choise.. 1 - Japanese Straightening is a day trip to the salon. Granted it is fabulous, but I can take upwards of 5 hours depending on the length & texture of your hair.. 2 - With Japanese Straightening you cannot have any other chemical services done. So its either you want it Straight, or you want to have fun with color. 3 - Japanese Starightening can be up to $1500, where as Brazilian straightening is usually about $250. 4 - Brazilian straightening is coating, it does not break and restructure the disulfide bonds in your hair, as Japanese Straightening does.. 5 - With Brazilian you can color your hair, which should be done a week before, as to not strip the straightener out of your hair.. 6 - Thr Brazilian ...
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Enabling investigators to establish stem cell lines in their laboratories is the primary goal of the WiCell Stem Cell Bank. To this aim, WiCell provides protocols for every available stem cell line. The protocols are also shipped with every vial of material. Many cell lines are available in multiple culture platforms (i.e., combinations of medium and matrix). Culture platforms and associated protocols for each cell line are accessible when viewing cell line specific information.
The book describes strategies to exploit chemical cues in cell differentiation processes with emphasis on purification and characterization of exosomes and secreted vesicles, plus micro and non-coding RNAs that have been demonstrated to control the tuning of the in vivo micro and macro environment.
Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of
Engineering cellular microenvironments that more accurately reflect the in vivo situation is now recognized as being crucial for the improvement of the in vitro viability and in vivo-like function of cells or tissues. Microfluidic technologies have been increasingly applied since the late 1990s for this purpose, with a growing number of examples of perfused cell and tissue cultures in microfluidic chambers and channels. The well-defined solution flows provided by microfluidics mean enhanced cell growth and function through improved nutrient delivery and waste removal. Additional benefits include the implementation of well-defined temporal and spatial (bio)chemical gradients, and mechanical signals that cells experience in their natural environment. Because the ability to culture cells and tissue under such controlled conditions leads to cellular function that is distinctly more organ-like, the microfluidic systems used are now referred to as organs-on-a-chip or microphysiological systems. ...
GD-OCM combines high sectioning capability of optical coherence tomography (OCT) and high lateral resolution of confocal microscopy to achieve high-contrast imaging with volumetric cellular resolution of 2 μm across a thickness of up to 2 mm in tissue. The current GD-OCM system fits on a movable cart. The handheld scanning probe is attached to an articulated arm that can be easily adjusted. The light source is a superluminescent diode laser centered at 840 nm with 100 nm FWHM (BroadLighter D-840-HP-I, Superlum®, Ireland). The microscope objective probe incorporates a liquid lens, which allows dynamic-focusing in order to image different depths of the sample. A custom dispersion compensator and a custom spectrometer with a high-speed CMOS line camera (spl4096-70km, Basler Inc.) are used to acquire the spectral information. We recently implemented and will also present a parallelized Multi-Graphic Processing Units (GPU) architecture of the system to allow real-time visualization of the sample. ...
Cecilia Sahlgren is a part-time Associate Professor at the TU/e department of Biomechanical Engineering (research group Soft Tissue Engineering & Mechanobiology) and Professor of Cell Biology at Åbo Akademi University in Turku, Finland. Her research aims at understanding the basic molecular principles of signaling mechanisms regulating cell fate choices of stem cells in cardiovascular tissue engineering. Another important goal is to develop technology to specifically monitor and tune these signals at will in specific cell populations, in order to steer stem cell fate and curtail disease activities. The specific focus of her research is on the role and regulation of the evolutionary conserved Notch signaling pathway, a key regulator of stem cell function. The main objectives are: 1) to understand how the cellular microenvironment influences Notch signaling activities and how this impinges on cell identity and function 2) to understand how Notch activity in stem cells influence remodelling of the ...
Research Summary: Current fibrin matrices/sealants used for wound healing applications utilize high concentrations of fibrinogen and thrombin, forming a dense matrix in order to form a stable clot. We have designed materials to be incorporated into fibrin scaffolds that will create a mechanically robust clot, but allow for increased cell infiltration and regeneration. As a member of the IGERT program and Thomas Barkers Laboratory of Matrix Biology and Engineering, the overall goals of my current research are to engineer fibrin networks to promote endogenous progenitor cell homing and infiltration for enhanced wound healing and tissue regeneration. Through incorporating ultra low cross-linked pNIPAm microgels into fibrin gels, we can modulate the matrix architecture and mechanical properties of the local cellular microenvironment. Additionally, we aim to load microgels with chemokines to recruit specific cell populations to sites of tissue injury. Through harnessing the chemistry and physics of ...
Many forms of solid tumor have a characteristic feature known as hypoxia, which describes a low or non-existent presence of oxygen in the cellular microenvironment. This decrease in oxygen causes activation of the hypoxia inducible factor (HIF) pathway, which activates the transcription of many genes that cause cell proliferation, metastasis, increased glycolysis and angiogenesis. Increased HIF expression has been linked with poor patient prognosis, increased malignancy, and therapeutic resistance. Previous work in our lab has identified 1 and 2 as inhibitors of the HIF pathway, specifically as disrupters of the p300-HIF-1α complex formation. A library of sulfonamide analogs has been designed and synthesized with the intent of examining the SAR of this series of compounds and improving potency and physicochemical properties as compared with lead compounds 1 and 2. At the end, we have achieved a thorough understanding of the structural features critical for future optimization work ...
Delta-like 4 (Dll4) is a ligand of the Notch pathway family which has been widely studied in the context of tumor angiogenesis, its blockade shown to result in non-productive angiogenesis and halted tumor growth. As Dll4 inhibitors enter the clinic, there is an emerging need to understand their side effects, namely the systemic consequences of Dll4:Notch blockade in tissues other than tumors. The present study focused on the effects of systemic anti-Dll4 targeting in the bone marrow (BM) microenvironment. Here we show that Dll4 blockade with monoclonal antibodies perturbs the BM vascular niche of sub-lethally irradiated mice, resulting in increased CD31(+), VE-Cadherin(+) and c-kit(+) vessel density, and also increased megakaryocytes, whereas CD105(+), VEGFR3(+), SMA(+) and lectin(+) vessel density remained unaltered. We investigated also the expression of angiocrine genes upon Dll4 treatment in vivo, and demonstrate that IGFbp2, IGFbp3, Angpt2, Dll4, DHH and VEGF-A are upregulated, while FGF1 and CSF2
Bioinspired, Non-Covalent Assembly of Materials: Stem cell activities, including self-renewal and differentiation, are strongly influenced by signals present in their microenvironment. Our group is using novel materials chemistry to control the signals present in the local stem cell microenvironment. Our approaches use non-covalent interactions between biological molecules (e.g. proteins, DNA strands) to assemble signaling complexes on cell culture substrates and within extracellular matrices. These non-covalent assembly approaches are being used to understand/control stem cell differentiation and as a mechanism for targeted drug delivery.. Bio-responsive Materials: Biological macromolecules, including proteins and poly(nucleotides), provide the most basic unit of function in living systems. For example, proteins fold to give structurally well-defined three-dimensional macromolecules and in many cases undergo highly complex changes in response to a broad spectrum of environmental cues, including ...
School of Basic Medical Science, Nanjing Medical University - Cited by 1,347 - Brain Tumor Stem Cells Microenvironment Metabolism
BlackRock Income Trust, Inc.s (BKT) (the Trust) investment objective is to manage a portfolio of high-quality securities to achieve both preservation of capital and high monthly income. The Trust seeks to achieve its investment objective by investing at least 65% of its assets in mortgage-backed securities. The Trust invests at least 80% of its assets in securities that are (i) issued or guaranteed by the US government or one of its agencies or instrumentalities or (ii) rated at the time of investment either AAA by S&P or Aaa by Moodys. The Trust may invest directly in such securities or synthetically through the use of derivatives.No assurance can be given that the Trusts investment objective will be achieved. .carousel-header-text { font: 24px/24px AkkuratProRegular, Arial, sans-serif; text-align: center; margin-top: 20px; float: left; } .carousel-subheader { font: 20px/220x AkkuratProRegular, Arial, sans-serif; text-align: center; } .carousel-text { font: 16px/16px AkkuratProRegular,
Dr. Mullen current research work examines the bone marrow microenvironment of acute lymphoblastic leukemia. The current goal of the work is to identify the molecular mechanisms by which nonmalignant cells in the marrow microenvironment that provide antiapoptotic support for leukemia cells. Ultimately this may identify new drug-able targets that may improve leukemia therapy. ...
While most salon do offer Japanese perm, Daisuke Salon knows the proper techniques needed to make the perfect perm. Our stylists are continuously trained by a Japanese stylist on the latest techniques, styles and fashion.. ...
A new cell culture platform allows researchers to observe never-before-seen behaviors of live cancer cells under the microscope, leading to explanations of long-known cancer characteristics.
chains in the Genus database with same CATH superfamily 2KMC A; 3ONS A; 1WXS A; 4DHZ E; 3APD A; 2Z0D B; 3APC A; 1WM2 A; 4IM2 A; 3SU8 X; 4HVB A; 2REX A; 4GN1 A; 3PZD A; 4ZRK A; 2LXC A; 4BKS D; 4GXB A; 4ZDV A; 2BYF A; 2DAF A; 2KDI A; 2L8J A; 3N32 A; 5IA7 A; 3WAN A; 2V3S A; 3WAM A; 4A3O A; 4EZJ A; 4KSK C; 2KTF A; 1V2Y A; 1V86 A; 4BKT A; 2L0T A; 4HPL B; 1WMH A; 3K9P B; 2GBK A; 4RMA A; 4FJV B; 5FR6 A; 3WAL A; 4AWJ A; 3T8M A; 2KXJ A; 2N9E B; 3I3T B; 3ZNZ B; 4NQK E; 3MJW A; 3RD2 A; 2HTH A; 4G11 A; 3R3M A; 1UZX B; 2KQS A; 3RUI B; 1WE6 A; 4JL9 A; 2KTR A; 2IO2 B; 2LJ5 A; 1WM3 A; 3DVN V; 4WAA A; 5J2R A; 2MLB A; 2D3G A; 2K6Q A; 2DYO A; 3ZUN A; 2JXX A; 4MDK E; 2K8C A; 2KN5 A; 4HXD A; 2KNB A; 4W9L A; 3APF A; 2L7R A; 2N1V A; 1Q0W B; 2ZVO A; 3IVF A; 2BKF A; 3RZF A; 3CSF A; 3L16 A; 3PHX B; 1Q1O A; 3PTF C; 2UWQ A; 3DPD A; 4BFR A; 4DDI D; 3QAQ A; 2JUO A; 2KJ6 A; 2IYD B; 2KC2 A; 1WX8 A; 2L76 A; 3QAR A; 1E8W A; 4MEM A; 5D6J B; 1EF5 A; 3UQA A; 1IP9 A; 2ZEQ A; 4MJS A; 2XEW A; 2J7Q B; 3A9K A; 1JRU A; 2FCN A; 2N4F A; ...
TY - JOUR. T1 - Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. AU - Nguyen, Aaron N.. AU - Stebbins, Elizabeth G.. AU - Henson, Margaret. AU - OYoung, Gilbert. AU - Choi, Sun J.. AU - Quon, Diana. AU - Damm, Debby. AU - Reddy, Mamatha. AU - Ma, Jing Y.. AU - Haghnazari, Edwin. AU - Kapoun, Ann M.. AU - Medicherla, Satyanarayana. AU - Protter, Andy. AU - Schreiner, George F.. AU - Kurihara, Noriyoshi. AU - Anderson, Judy. AU - Roodman, G. David. AU - Navas, Tony A.. AU - Higgins, Linda S.. PY - 2006/6/10. Y1 - 2006/6/10. N2 - The multiple myeloma (MM) bone marrow (BM) microenvironment plays a critical role in supporting tumor growth and survival as well as in promoting formation of osteolytic lesions. Recent results suggest that the p38 mitogen-activated protein kinase (MAPK) is an important factor in maintaining this activated environment. In this report, we demonstrate that the p38α ...
MicroRNAs: Novel Crossroads between Myeloma Cells and the Bone Marrow Microenvironment. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Edwin L. Steele Laboratory for Tumor Biology. Research Summary. Bench to Bedside and Back. As part of the Edwin L. Steele Laboratory for Tumor Biology, research at the Jain Laboratory focuses on normalizing tumor vessels and microenvironment. A solid tumor is an organ composed of cancer cells and host stromal cells, which are nourished by the vasculature and embedded in an extracellular matrix. The interaction among these cells, the surrounding matrix, and the local cellular microenvironment influences the expression of certain genes, whose products control the pathophysiological characteristics of the tumor, govern tumor progression, and affect the tumors response to various therapies. Blood and lymphatic vessels also serve as conduits for metastatic spread. The over arching goal of our research is to dissect the pathophysiology of the vascular and extra-vascular components of tumors, to determine the role of tumor-host interactions in tumor biology, and ultimately to translate this knowledge ...
TY - JOUR. T1 - Determination of cytokine regulated glycan expression by using molecularly imprinted polymers targeting sialic acid. AU - Shinde, Sudhirkumar. AU - El-Schich, Zahra PY - 2019/7/11. Y1 - 2019/7/11. N2 - Cancer cells often have an increased amount of glycans, such as sialic acid (SA), on the cell surface, which normallyplay an important role in cell growth, proliferation and differentiation. In this study, SA expression is determinedby fluorescent nanoprobes, molecularly imprinted polymers, SA-MIPs. The nanoprobes are synthesized with animprinting approach to produce tailor-made fluorescent core-shell particles with high affinity for cell surface SA.Inflammation and cytokine production are well known tumor promoters, modulating the cellular microenvironment,including an aberrant cell surface glycan pattern. The recombinant cytokines IL-4, IL-6, IL-8 and a cocktail ofcytokines collected from stimulated T leukemia Jurkat cells were used to induce in vitro inflammation in two ...
ATF6, a membrane-anchored transcription factor from the endoplasmic reticulum (ER) that modulates the cellular response to stress as an effector of the unfolded-protein response (UPR), is a key player in the development of tumors of different origin. reticulum (ER) can be particularly affected by the presence of mutations in secretory proteins or by dynamic changes in the cellular microenvironment, events which are often encountered in cancers. In the ER, these events are sensed by specific sensors, which in turn trigger select Rabbit Polyclonal to CPB2 signaling pathways, collectively named the unfolded-protein response (UPR) (1). The UPR is an adaptive response that allows the cells to either overcome the stress or promote cell death in the case of overwhelming burden (1). Three ER-resident proteins, namely, the protein kinase PKR-like ER kinase (PERK), the inositol-requiring protein 1 alpha (IRE1), and the activating transcription factor 6 alpha (ATF6), have been identified as the major ...
The VHL−/− human RCC cell line 786-O forms tumors in the nude mouse xenograft assay. We showed previously that reintroduction of pVHL into these cells markedly inhibited the ability of these cells to grow as tumors in nude mice (8, 9) without altering their growth characteristics in vitro. These observations led to the hypothesis that the cellular microenvironment participates in pVHL-mediated tumor suppression possibly through regulation of hypoxia-inducible genes and angiogenesis (11). RCC cells lacking wild-type pVHL inappropriately overproduce hypoxia-inducible mRNA under both hypoxic (1% ambient O2) and normoxic (21% ambient O2) conditions (8, 9). Reintroduction of pVHL into these cells restores the normal profile of hypoxia-inducible mRNA regulation and leads to tumor suppression.. The overexpression of hypoxia-inducible genes in VHL−/− cells has been attributed to transcriptional and posttranscriptional events (8, 9), implying that the effects of pVHL could be attributed to ...
Inflammation in obese adipose tissue can be modulated by alterations of the composition and/or interactions of local immune cells. Our finding that CD40KO mice in the DIO model exhibited exacerbated local inflammation in EAT, insulin resistance, and metabolic abnormalities was initially surprising, as CD40 itself is a costimulatory receptor and its deficiency impairs general immune responses. Of particular interest, we found that CD40-deficient CD8+ T cells in EAT produced more proinflammatory cytokines and CCL5 and CCL3, and this was critical for immune cell infiltration and insulin resistance induction in DIO CD40KO mice. We propose that CD40 may play a special role in the microenvironment of obese EAT through regulating CD8+ T-cell function.. In contrast to our observations with CD40KO mice, a previous study showed that CD154/CD40L is a requirement to maintain adipose tissue inflammation in mice (24) and CD154 deficiency attenuates diet-induced steatosis and improves insulin resistance (28). ...
has been shown in mice that enhanced tonus of vegetative nervous system regulates mobilization of hematopoietic stem and progenitor cells (HSPCs) into peripheral blood (PB). also mobilized into PB in an enforced manner by administration of granulocyte-colony stimulating element (G-CSF) and it has also been shown in mice that launch of these cells into PB depends critically within the vegetative nervous system.4 5 Moreover UDP-galactose:ceramide galactosyltransferase-deficient Rabbit Polyclonal to GUF1. mice which show aberrant nerve conduction and dont launch norepinephrine (NE) into the BM microenvironment do not mobilize HSPCs in response to G-CSF. 4 To explain how NE signaling influences HSPC mobilization it has been postulated that it modulates manifestation of stromal derived factor-1 JTP-74057 (SDF-1) in the BM microenvironment and such a mechanism would be consistent with the finding that administration of β2-adrenergic agonists enhances mobilization of HSPCs in both control and ...
Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System. The Hyperion Imaging System brings proven CyTOF® technology together with imaging capability to empower simultanous interrogation of 4 to 37 protein markers using Imaging Mass Cytometry™ (IMC™). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future. ...
Define microenvironment: a small or relatively small usually distinctly specialized and effectively isolated habitat (such as a forest canopy) or…
A sample processing system that may be configured to achieve rapid sample processing such as rapid histochemical processing may involve a wave element that can use angular microscopic slide movements to cause repeated elimination and reapplication of a fluidic substance perhaps through the action of capillary motion in order to refresh a microenvironment adjacent to a sample such as a biopsy or other such sample. Through such refreshing of a microenvironment, depletion of the microenvironment is avoided and the time necessary for slide processing may be dramatically shortened.
"The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... "Lysosomal-mediated degradation of apoptotic thymocytes within thymic nurse cells." Cellular immunology 197.2 (1999): 108-115. ... "The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... "The thymus microenvironment in regulating thymocyte differentiation." Cell adhesion & migration 4.3 (2010): 382-390. Nakagawa, ...
Cellular Microbiology. 9 (10): 2315-22. doi:10.1111/j.1462-5822.2007.00995.x. hdl:2027.42/73608. PMID 17587328. Chaudhari, ... "Lactate and Acidity in the Cancer Microenvironment". Annual Review of Cancer Biology. 4: 141-158. doi:10.1146/annurev-cancerbio ...
"Reactive Oxygen Species Regulate T Cell Immune Response in the Tumor Microenvironment". Oxidative Medicine and Cellular ... Cellular transformation leads to cancer and interaction of atypical PKC-ζ isoform with p47phox controls ROS production and ... ROS are intrinsic to cellular functioning, and are present at low and stationary levels in normal cells. In vegetables, ROS are ... Moreover, high inducers of ROS such as 2-deoxy-D-glucose and carbohydrate-based inducers of cellular stress induce cancer cell ...
Mcnairn, AJ; Guasch, G (2011). "Epithelial transition zones: merging microenvironments, niches, and cellular transformation". ...
If cellular automata rules have not been formulated from the first principles of the subject, then such a model may have a weak ... Ch'ng, E. (2012) Macro and Micro Environment for Diversity of Behaviour in Artificial Life Simulation, Artificial Life Session ... The idea intrigued von Neumann, who drew it up-creating the first of the devices later termed cellular automata. Another ... The deterministic logical cellular automata are necessary but not sufficient condition of a white-box model. The second ...
The Centre focuses on cellular matrix microenvironment, matrix immunobiology, and mechanobiology of the matrix. As of 2016[ ...
This micro-environment acidification can lead to cellular stress, which would lead to autophagy. Autophagy is activated in ... and during certain cellular developmental stages, such as during cellular differentiation of red blood cells. Organelles and ... Additionally, in the microenvironment of cancer cells, there is an increase in hypoxia-inducible transcription factor 1-alpha ( ... Disorders in energy creation by mitochondria can cause cellular degeneration, like those seen in the substantia nigra. ...
Chen has used his insights in cellular microenvironments to develop strategies to engineer tissue assembly. He has advocated ... how 3D culture microenvironments alter cellular cues". Blakely, B. L.; Dumelin, C. E.; Trappmann, B.; McGregor, L. M.; Choi, C ... engineered cellular micro-environments, micro-electromechanical systems and micro-fabrication technologies. Chen has been ... where he has made contributions in cellular microenvironments, tissue assembly, and vascular biology. Throughout these studies ...
Moreover, they also need to provide the appropriate microenvironment for vessel ingrowth and cellular proliferation. Polymers ...
Furthermore, antigen transfer enables TRA processing and presentation by different cellular microenvironments. Despite ... Koble C, Kyewski B (July 2009). "The thymic medulla: a unique microenvironment for intercellular self-antigen transfer". The ... "processing of TRAs by different microenvironments". N.B.: BM derived APCs don´t express TRAs, this process is uniquely ...
... precise control of cellular microenvironment, as well as controlled integration of cells into appropriate multi-cellular ... Microfluidics have been applied in these technologies to better mimic the in vivo microenvironment with patterned topographic ... Flaim, Christopher J; Chien, Shu; Bhatia, Sangeeta N (2005). "An extracellular matrix microarray for probing cellular ... Rosenthal, Adam; Macdonald, Alice; Voldman, Joel (2007). "Cell patterning chip for controlling the stem cell microenvironment ...
In this area, he has worked on disrupting CC-CAF crosstalk and abrogating the key cellular components participating in ... Mukherjee's primary area of research revolves around tumor and tumor microenvironments. His research also deals with protein- ... Some of Mukherjee's research focuses on reprogramming tumor microenvironments. ... ". "Gold Nanoparticle Reprograms Pancreatic Tumor Microenvironment and Inhibits Tumor Growth". "Reprogramming Tumor ...
Various integrin receptors monitor the mechanical properties of the cellular microenvironment and can influence and initiate ... Podosomes are thought to be intimately connected to cellular motility within tissue microenvironments through coordinating ... immune systems do not manifest podosome formations and demonstrate defects in cellular movement within tissue microenvironments ... Their primary purpose is connected to cellular motility and invasion; therefore, they serve as both sites of attachment and ...
She is a senior investigator and head of the tumor microenvironment section at the National Cancer Institute. Yang received a ... Her research approaches include cellular and molecular biology, cancer biology, immunology, as well as integrated genomic-wide ... As head of the tumor microenvironment Section, Yang is interested in how inflammation in the premetastatic environment modifies ... She investigated TGF-β regulation of inflammation and tumor microenvironment as a postdoctoral researcher research with Harold ...
The gap junction had been shown to create a micro-environment between the two cells in the extra-cellular space or "gap". This ... Tissues in this section have well known functions observed to be coordinated by gap junctions with inter-cellular signaling ... The recently characterized pannexin family, which was originally thought to form inter-cellular channels (with an amino acid ... Robertson, J. D. (1963). Locke, Michael (ed.). Cellular membranes in development. New York: Academic Press. OCLC 261587041.[ ...
These functions have not been well characterized and the effect of the extracellular microenvironment on their function is also ... More specifically, they are involved in several cellular functions, including proliferation and migration of neural stem cells ...
Cellular uptake of certain small molecule drugs is also increased. Very high temperatures, above 50 °C (122 °F), are used for ... However, the vascular disorganization of a solid tumor results in an unfavorable microenvironment inside tumors. Consequently, ...
... is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free ... Additionally, several types of cells in the microenvironment of cancer may change their phenotypes to support cancer growth. ... Moreover, generation of the canonical and non-canonical dimers, viz RelA:p50 and RelB:p52, within the cellular milieu are ... NF-κB is important in regulating cellular responses because it belongs to the category of "rapid-acting" primary transcription ...
Staudt MR, Kanan Y, Jeong JH, Papin JF, Hines-Boykin R, Dittmer DP (July 2004). "The tumor microenvironment controls primary ... "Distinct subsets of primary effusion lymphoma can be identified based on their cellular gene expression profile and viral ...
Cellular dormancy refers to the cell entering a state of quiescence where growth is arrested in G0-G1 of the cell cycle, and ... The signaling most likely comes from the microenvironment. The switch seems to be mediated by interactions between surface ... The ERK pathway has a major role in many cellular processes, but in cancer dormancy it is thought to be involved in mitogenic ... Cancer dormancy can refer to two different types: tumor mass dormancy and cellular dormancy. In tumor mass dormancy, the tumor ...
Cellular level: describe how geometrical, biochemical or mechanical cues can be integrated at the cellular level to influence ... These experiments rely on physical approaches to change and control the cell microenvironment in a precise and refined manner. ... Microscopy: The DEEP laboratory depends greatly on optical imaging and image analysis to measure the dynamics of cellular ... we obtain quantitative descriptions of the cellular properties. Emphasis is put on studying membrane remodeling. ...
There is some evidence that HIFU can be applied to cancers to disrupt the tumor microenvironment and trigger an immune response ... Thermal doses of 120-240 min at 43 °C coagulate cellular protein and leads to irreversible tissue destruction. ... "Effect of acoustic standing waves on cellular viability and metabolic activity". Scientific Reports. 10 (1): 8493. Bibcode: ...
In the extreme case of starvation, the breakdown of cellular components promotes cellular survival by maintaining cellular ... to recycle vital molecules and generate a pool of building blocks to help the cell respond to a changing microenvironment. ... Cellular autophagic machinery also play an important role in innate immunity. Intracellular pathogens, such as Mycobacterium ... Deter RL, De Duve C (May 1967). "Influence of glucagon, an inducer of cellular autophagy, on some physical properties of rat ...
... has made significant contributions to the understanding of the cellular and molecular biology of the tumour microenvironment ... Hodivala-Dilke's research goal is to study how the tumour microenvironment can control tumour progression and cancer treatment ... She is Professor of Angiogenesis and the Tumour Microenvironment and Deputy Institute Director of Barts Cancer Institute, Queen ...
... and cellular basis of neurofibromatosis. They are employing these preclinical mouse models to better understand the cellular ... origins of tumors, the contribution of the tumor microenvironment to tumor formation and growth, and the major growth control ...
... where diffusion of cellular products was restricted to inside a small closed culture. Her first success in duplicating an ... where single cells could be picked up and isolated from under the microscope and placed in a detailed microenvironment, ... serving as head of Laboratory of Cellular and Molecular Biology. In the 1940s she became the first person to successfully clone ... Sanford was further promoted to the head of the in-vitro carcinogenesis section of the Laboratory of Cellular and Molecular ...
Rothlin's lab also explores the role TAMs play in cellular immunology and innate immune checkpoints in cancer. Immune ... to enhance immunogenicity of the tumor microenvironment and cytotoxicity tumor infiltrating cells. Rothlin and her team are ... and cellular crosstalk in the context of injury and cell turnover. She has made fundamental discoveries about the roles of TAM ... checkpoint blockade therapy has historically focused on the adaptive immune system, blocking the cellular breaking system on T ...
Apparently, the cellular elements of the alveolar structures differ from tumor cells of other structures by a set of biological ... These blebs allow the cell to investigate the microenvironment to find the most suitable route of movement to bypass various ... 1). This is the main feature of this type of invasion, since the underlying cellular mechanisms are the same key processes that ... 1). In this case, the migration type is largely determined by tissue microenvironment features and depends on molecular changes ...
... sensory-motor adjustments after the visual field has been artificially shifted Cellular adaptation, changes by cells/tissues in ... response to changed microenvironments High-altitude adaptation, organisms and their specializations for life in high altitudes ...
In those with healthy or preserved SSCs but without a cellular environment to support them, in vitro spermatogenesis could be ... These include creating a similar microenvironment to that of the testis as well as supporting endocrine and paracrine ... but Leydig and peritubular myoid cells both contribute to the microenvironment that encourage stem cells to remain pluripotent ... fragment/3D cultures are able to establish in vitro conditions that can somewhat resemble the testicular microenvironment to ...
This is due to the accumulation of oxidative damage to DNA by aging and cellular metabolic activity and the shortening of ... Lefebvre JS, Maue AC, Eaton SM, Lanthier PA, Tighe M, Haynes L (2012). "The aged microenvironment contributes to the age- ... Cambier, J. (2005). "Immunosenescence: a problem of lymphopoiesis, homeostasis, microenvironment, and signaling". Immunological ...
Carol Greider, who in 1992 discovered a relationship between cellular aging and damage to the ends of chromosomes, called ... Research foci: drug resistance; cancer genomics; tumor microenvironment; growth control in mammalian cells; transcriptional and ...
Chanmee T, Ontong P, Itano N (May 2016). "Hyaluronan: A modulator of the tumor microenvironment". Cancer Letters. 375 (1): 20- ... "Targeting the tumor microenvironment in cancer: why hyaluronidase deserves a second look". Cancer Discovery. 1 (4): 291-6. doi ... Molecular and Cellular Biology portal. Retrieved from "https://en.wikipedia.org/w/index.php?title=Hyaluronidase&oldid=891224674 ...
cellular response to drug. • cell cycle arrest. • learning or memory. • cellular copper ion homeostasis. • cellular response to ... These interfaces allow specific sets of cells to communicate based on level of expression and the surrounding microenvironment ... Cellular component. • mitochondrial outer membrane. • membrane. • mitochondrion. • integral component of membrane. • cytoplasm ... CellularEdit. Varying expression of PrP through the cell cycle has led to speculation on involvement in development. A wide ...
Such effects on cellular and physiological phenotypic traits may result from external or environmental factors, or be part of ... This supports the notion that microenvironment at early stages of embryonic development can be quite important for the ... One example of an epigenetic change in eukaryotic biology is the process of cellular differentiation. During morphogenesis, ... This might suggest that plant cells do not use or require a cellular memory mechanism and just respond to positional ...
... of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment ... By using deep sequencing of DNA and RNA from a single cell, cellular functions can be investigated extensively.[1] Like typical ... However, both methods require detaching cells from their microenvironments first, thereby causing perturbation to the ...
Cellular component. • cytoplasm. • cytosol. • nuclear membrane. • membrane. • nuclear matrix. • nuclear envelope lumen. • cell ... "Deletion of 5-Lipoxygenase in the Tumor Microenvironment Promotes Lung Cancer Progression and Metastasis through Regulating T ... or are the cellular receptors responsible for mediating the cellular responses to the down-stream ALOX products LTC4 and LTD4 ( ... Hu Y, Li S (2016). "Survival regulation of leukemia stem cells". Cellular and Molecular Life Sciences. 73 (5): 1039-50. doi: ...
Cellular & Molecular Immunology. 1 (2): 95-104. PMID 16212895.. *^ a b Graham GJ, Locati M (January 2013). "Regulation of the ... constitutively produced in the bone marrow promotes proliferation of progenitor B cells in the bone marrow microenvironment.[4] ... or guide cells to tissues that provide specific signals critical for cellular maturation. Other chemokines are inflammatory and ...
Smith JA, Francis SH, Corbin JD (Nov 1993). "Autophosphorylation: a salient feature of protein kinases". Molecular and Cellular ... Addition of a negatively charged phosphate group brings about a change in the microenvironment that may lead to attraction or ... It will therefore be inaccessible to cellular substrates. In response to DNA damage, the kinase domain of one monomer ... Protein kinases, many of which are regulated by autophosphorylation, are vital in controlling the cellular proliferation, ...
"Molecular and Cellular Biology. 13 (12): 7708-17. doi:10.1128/mcb.13.12.7708. PMC 364842. PMID 8246987.. ... PIK3CA participates in a complex interaction within the tumor microenvironment in this phenomenon.[12] ... Cellular component. • phosphatidylinositol 3-kinase complex. • cytosol. • phosphatidylinositol 3-kinase complex, class IA. • ... regulation of cellular respiration. • T cell receptor signaling pathway. • activation of protein kinase activity. • regulation ...
"Molecular and Cellular Biology. 23 (7): 2225-38. doi:10.1128/MCB.23.7.2225-2238.2003. PMC 150734. PMID 12640109.. / ... angiogenesis and migration of cancer cells by influencing the tumor microenvironment.[66][67] Oncogenes build up an ... The improved understanding of molecular biology and cellular biology due to cancer research has led to new treatments for ... inflammatory pro-tumorigenic microenvironment.[68] Hormones. Some hormones play a role in the development of cancer by ...
See homeostasis). Cellular theory of ageing can be categorized as telomere theory, free radical theory (free-radical theory of ... that age-dependent accumulation of DNA damage in both stem cells and cells that comprise the stem cell microenvironment is ... The stem cell theory of aging is also a sub-category of cellular theories. Smith J., A., Daniel R. "Stem Cells and Aging: A ... Aging of the hair follicle appears to be primed by a sustained cellular response to the DNA damage that accumulates in renewing ...
cellular response to lithium ion. • cell adhesion. • extracellular matrix organization. • response to drug. • cellular response ... The mesenchymal state cancer cells migrate to new sites and may undergo METs in certain favorable microenvironment. For example ... Cellular component. • cell-cell adherens junction. • apical junction complex. • trans-Golgi network. • extracellular region. • ... "Molecular and Cellular Biology. 23 (7): 2287-97. doi:10.1128/MCB.23.7.2287-2297.2003. PMC 150740. PMID 12640114.. ...
Immune modulation of the tumor microenvironment for enhancing cancer immunotherapy. „Oncoimmunology". 2 (8), s. e25961, ... T cell proliferation in cow's milk allergy after CD25+ regulatory T cell removal suggests a role for casein-specific cellular ...
In a murine air pouch model of carrageenan/IL-1-induced inflammation, HA was observed to enhance cellular infiltration.[26][27] ... This may contribute to the hydrated microenvironment at sites of synthesis, and is essential for cell migration by facilitating ... They are round-shaped with rounder nuclei, and have less elongated cellular processes when compared with fibroblasts. ...
... others believe that the less challenging collection techniques and the multi-cellular microenvironment already present in ... Cellular and Molecular Life Sciences. 70 (20): 3871-3882. doi:10.1007/s00018-013-1290-8. hdl:1822/25128. ISSN 1420-682X. PMID ... Viral entry into CD4+ cells is mediated by the interaction with a cellular chemokine receptor, the most common of which are ... These cells involved in the secondary damage response secrete factors that promote scar formation and inhibit cellular ...
disease of cellular proliferation?[1] և Մկանուռուցք. Պատճառ. Ժառանգական նախատրամադրվածություն, միջավայրի վնասակար գործոններ, ... Interaction of tumor cells with the microenvironment»։ Cell Communication and Signaling 9 (18): 18։ September 2011։ PMC 3180438 ... Molecular and Cellular Biology 23 (7): 2225-38։ April 2003։ PMC 150734։ PMID 12640109։ doi:10.1128/MCB.23.7.2225-2238.2003 , ...
... others believe that the less challenging collection techniques and the multi-cellular microenvironment already present in ... Viral entry into CD4+ cells is mediated by the interaction with a cellular chemokine receptor, the most common of which are ... These cells involved in the secondary damage response secrete factors that promote scar formation and inhibit cellular ... Because subsequent viral replication requires cellular gene expression processes, activated CD4+ cells are the primary targets ...
9,0 9,1 Abbas AK and Lichtman AH (2003). Cellular and Molecular Immunology (trükk: 5th). Saunders, Philadelphia. ISBN 0-7216- ... Eitan Yefenof, Innate and Adaptive Immunity in the Tumor Microenvironment, ISBN: 978-1-4020-6749-5, 2008, Springer Science + ... Alberts B, Johnson A, Lewis J, et al., Lymphocytes and the Cellular Basis of Adaptive Immunity, Molecular Biology of the Cell, ...
The microscopic cellular characteristics of the tumor are reported from the analysis of tissue taken from a biopsy or surgery. ... pre-neoplastic cells but also influences of exogenous environmental factors and molecular changes in the local microenvironment ... cellular pleomorphism, and mucosecretion of the predominant pattern, adenocarcinoma may present three degrees of ...
So, WK; Cheung, TH (2018). Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches. ... Anti-inflammatory effects on the local microenvironment, which promote tissue healing, are also observed. The inflammatory ...
Injury-induced stem-cell niche are defined as cellular microenvironments that are generated during tissue injury, these ...
2001). "Versican interacts with chemokines and modulates cellular responses.". J. Biol. Chem. 276 (7): 5228-34. PMID 11083865. ... 2002). "Influence of the mucosal epithelium microenvironment on Langerhans cells: implications for the development of squamous ... "Human B cells become highly responsive to macrophage-inflammatory protein-3 alpha/CC chemokine ligand-20 after cellular ...
If present on a super-cellular scale, biominerals are usually deposited by a dedicated organ, which is often defined very early ... These composites are often not only as hard as the pure mineral but also tougher, as the micro-environment controls ... Boskey, A. L. (1998). "Biomineralization: conflicts, challenges, and opportunities". Journal of Cellular Biochemistry. ...
A cellular receptor, generally CCR5 or CXCR4 is required in order for HIV entry into CD4 cells. Cells of individuals homozygous ... "Sex Hormones Selectively Impact the Endocervical Mucosal Microenvironment: Implications for HIV Transmission". PLOS ONE. 9 (5 ... the cellular immune response, and the immune exhaustion as the infection progresses.[3] ...
This creates a tumor microenvironment that is short of blood vessels (hypovascular) and so of oxygen (tumor hypoxia).[2] It is ... based on their degree of cellular differentiation (from "NET G1" through to the poorly differentiated "NET G3").[20] The U.S. ... Still others aim to affect the non-neoplastic stroma and microenvironment of the tumor, which is known to influence cell ...
Kimelberg HK, Jalonen T, Walz W (1993). "Regulation of the brain microenvironment:transmitters and ions.". In Murphy S. ... and exhibit long unbranched cellular processes. This type often has "vascular feet" that physically connect the cells to the ...
2006). "Cancer progression by non-clonal chromosome aberrations". Journal of Cellular Biochemistry. 98 (6): 1424-1435. doi: ... The surviving cells might be in a special microenvironment, which protects them from adverse effects of treatment.[90] ... Driver mutation = a mutation that gives a selective advantage to a clone in its microenvironment, through either increasing its ... 2006). "Stochastic cancer progression driven by non-clonal chromosome aberrations". Journal of Cellular Physiology. 208 (2): ...
... scientists are able to manipulate the cellular microenvironment in order to simulate physiological conditions in vivo. ... Using cell cultures, scientists are able to manipulate the cellular microenvironment in order to simulate physiological ... www.news-medical.net/life-sciences/Controlling-the-Cellular-Microenvironment-in-vitro.aspx. (accessed August 13, 2020). ... www.news-medical.net/life-sciences/Controlling-the-Cellular-Microenvironment-in-vitro.aspx. ...
One major component of the tumor microenvironment is the immune system which has spawned a new and hopeful generation of cancer ... Understanding the network of communication between multiple cell types within the microenvironment is key to understanding how ... The tumor microenvironment is composed of a variety of different cell types that work in concert to support or limit the ... within the TME can be hijacked by factors expressed by tumor cells or by alternative cell types in the microenvironment, such ...
Besides cellular microenvironments with systematically altered single-GAG concentrations, matrices containing both low and high ... Bioinspired Collagen/Glycosaminoglycan-Based Cellular Microenvironments for Tuning Osteoclastogenesis.. Rother S1, Salbach- ... In multi-GAG microenvironments, atomic force microscopy revealed intermediate collagen fibril structures with thin fibrils and ...
Amperometric oxygen sensors were used to monitor cellular respiration with different incubation conditions. Cellular ... is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical ... Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask. Jochen Kieninger 1,* , Yaara Tamari 1. ... The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell ...
... in this proceeding volume reflects the latest research presented at the Aegean meeting on Tumor Microenvironment and Cellular ... in this proceeding volume reflects the latest research presented at the Aegean meeting on Tumor Microenvironment and Cellular ... The book provides critical insight to how the tumor microenvironment affects tumor metabolism, cell stemness, cell viability, ... Imaging Angiogenesis, Inflammation, and Metastasis in the Tumor Microenvironment with Magnetic Resonance Imaging ...
This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for ... Screening strategy to identify optimal cellular microenvironments for regulating cellular functions. (A) Overview of the ... Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array. doi: 10.3791/57377 Published: September 7, 2018 ... In recent years, an increasing number of studies have been conducted to reconstruct cellular microenvironments in vitro7,8. ...
Molecular & Cellular Proteomics April 1, 2004, First published on January 30, 2004, 3 (4) 327-344; DOI: 10.1074/mcp.M400009- ... Molecular & Cellular Proteomics April 1, 2004, First published on January 30, 2004, 3 (4) 327-344; DOI: 10.1074/mcp.M400009- ... Your Name) has sent you a message from Molecular & Cellular Proteomics Message Body (Your Name) thought you would like to see ... Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment. Julio E. Celis, Pavel Gromov ...
Importance of Cellular Microenvironment and Circulatory Dynamics in B Cell Immunotherapy. Qian Gong, Qinglin Ou, Shiming Ye, ... Importance of Cellular Microenvironment and Circulatory Dynamics in B Cell Immunotherapy. Qian Gong, Qinglin Ou, Shiming Ye, ... Importance of Cellular Microenvironment and Circulatory Dynamics in B Cell Immunotherapy. Qian Gong, Qinglin Ou, Shiming Ye, ... Importance of Cellular Microenvironment and Circulatory Dynamics in B Cell Immunotherapy Message Subject (Your Name) has ...
New findings presented today by CytoReason reveals possible new cellular players in the tumor microenvironment that could ... CytoReason: New Machine-Learning Driven Findings Uncover New Cellular Players in Tumor Microenvironment That Might Point the ... CytoReasons unique machine learning platform uncovers new cellular players in tumor microenvironment - new potential ... CytoReasons unique machine learning platform uncovers new cellular players in tumor microenvironment - new potential ...
This study aimed to further characterize the role of local microenvironment on tumor progression, with an emphasis on ... This study aimed to further characterize the role of local microenvironment on tumor progression, with an emphasis on ... and transplanted them into various in vivo microenvironments. We used immunohistochemistry to document the response of iSCs and ... and transplanted them into various in vivo microenvironments. We used immunohistochemistry to document the response of iSCs and ...
Detection of response to tumor microenvironment-targeted cellular immunotherapy using nano-radiomics ... Detection of response to tumor microenvironment-targeted cellular immunotherapy using nano-radiomics ... Detection of response to tumor microenvironment-targeted cellular immunotherapy using nano-radiomics ... Detection of response to tumor microenvironment-targeted cellular immunotherapy using nano-radiomics ...
The Centre for the Cellular Microenvironment (The CEMI). *The Centre for the Cellular Microenvironment (The CEMI) ...
The Centre for the Cellular Microenvironment (The CEMI). *The Centre for the Cellular Microenvironment (The CEMI) ...
Regulation of the endothelial barrier function: a filum granum of cellular forces, Rho-GTPase signaling and microenvironment. ... Beningo KA, Wang Y-L (2002) Flexible substrata for the detection of cellular traction forces. Trends Cell Biol 12:79-84PubMed ... Olofsson B (1999) Rho guanine dissociation inhibitors: pivotal molecules in cellular signalling. Cell Signal 11:545-54PubMed ... Wessells NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wrenn JT, Yamada K (1971) Microfilaments in cellular and ...
Abstract 2262: Dendrimer nanoparticle effects on the microenvironment: Examination of cellular motility, mitosis and ... Abstract 2262: Dendrimer nanoparticle effects on the microenvironment: Examination of cellular motility, mitosis and ... Abstract 2262: Dendrimer nanoparticle effects on the microenvironment: Examination of cellular motility, mitosis and ... Abstract 2262: Dendrimer nanoparticle effects on the microenvironment: Examination of cellular motility, mitosis and ...
Illinois BioNanotechnology Seminar Series Spring 2011: Engineering Cellular Microenvironments for Biomedicine By Brendan Harley ... Home › Online Presentations › Illinois BioNanotechnology Seminar Series Spring 2011: Engineering Cellular Microenvironments for ... Center for Cellular Mechanics (CCM). Cellular and Molecular Mechanics and BioNanotechnology Integrative Graduate Education and ... Brendan Harley (2011), "Illinois BioNanotechnology Seminar Series Spring 2011: Engineering Cellular Microenvironments for ...
Tumor microenvironment*. Footnotes. *. Author contributions: E.V.N., B.A.P., R.A.T., N.L.L., and R.D. designed research; E.V.N ... Your Name) has sent you a message from Molecular & Cellular Proteomics Message Body (Your Name) thought you would like to see ... Molecular & Cellular Proteomics May 6, 2019, mcp.RA119.001496; DOI: 10.1074/mcp.RA119.001496 ... Molecular & Cellular Proteomics May 6, 2019, mcp.RA119.001496; DOI: 10.1074/mcp.RA119.001496 ...
... Bianca Rodrigues da Cunha1,2*, Célia Domingos3,4*, ... Cellular Interactions in the Tumor Microenvironment: The Role of Secretome. J Cancer 2019; 10(19):4574-4587. doi:10.7150/jca. ... Journal of cellular physiology. 2009;219:563-71 114. Park YS, Hwang S, Jin YM, Yu Y, Jung SA, Jung SC. et al. CCN1 secreted by ... Molecular & cellular proteomics: MCP. 2018;17:901-12 31. Greaves M, Maley CC. Clonal evolution in cancer. Nature. 2012;481:306- ...
Genomic (In)stability of the Breast Tumor Microenvironment Seth Rummel, Allyson L. Valente, Jennifer L. Kane, Craig D. Shriver ...
Formation of Microenvironment, and Role of Soluble CD30 in Development of Adult T-Cell Leukemia/Lymphoma Abstract. ... A Lack of Cellular Senescence, Formation of Microenvironment, and Role of Soluble CD30 in Development of Adult T-Cell Leukemia/ ... Citation: Pornkuna R, Takemoto S (2014) A Lack of Cellular Senescence, Formation of Microenvironment, and Role of Soluble Cd30 ... It was implied that aberrant cellular proteins such as p53, JAK-STAT and Bcl-xL were associated with the development of ATL. ...
Deconstructing the third dimension - how 3D culture microenvironments alter cellular cues Message Subject (Your Name) has sent ... Deconstructing the third dimension - how 3D culture microenvironments alter cellular cues. Brendon M. Baker, Christopher S. ... Nanometer-scale features can also be used to alter the mechanical properties of the cellular microenvironment when this is ... Deconstructing the third dimension - how 3D culture microenvironments alter cellular cues. Brendon M. Baker, Christopher S. ...
Interplay of Substrate Conductivity, Cellular Microenvironment, and Pulsatile Electrical Stimulation toward Osteogenesis of ... defined chemical composition of cellular microenvironment, and intermittent delivery of electric pulses to drive mesenchymal ... which mimics the native microenvironment of bone tissue. Further, bone marrow derived human mesenchymal stem cells (hMSCs) were ...
Here the authors investigate the role of CaMKK2 expression in the tumour microenvironment and show that CaMKK2 expression in ... and facilitates a favorable reprogramming of the immune cell microenvironment. These data, credential CaMKK2 as a myeloid- ... 7d). Taken together, these studies define an important role for CaMKK2 in the regulation of cellular processes involved in the ... 9). Graph reports kinetics of OTI CD8+ T cells migrating toward 3D E0771-microenvironment infiltrated by WT or Camkk2−/− BMDM ( ...
Read our brief introduction to the tumor microenvironment (TME) covering its effects on angiogenesis, tumor immunology and ... Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists ... The tumor microenvironment (TME) is made up of a variety of support cells, which are subverted by the cancer cells to assist ... Roles of the tumor microenvironment during vascularization. Just as organs within the body require constant blood supply to ...
Stover DG, Bierie B, Moses HL (July 2007). "A delicate balance: TGF-beta and the tumor microenvironment". Journal of Cellular ... The tumor microenvironment is often hypoxic. As the tumor mass increases, the interior of the tumor becomes farther away from ... The tumor microenvironment (TME) is the environment around a tumor, including the surrounding blood vessels, immune cells, ... The interplay between the tumor and its microenvironment was part of Stephen Pagets 1889 "seed and soil" theory, in which he ...
Current, Local ECM Microenvironment Determines the Cellular Strategy of Invasion for Primary Human Mammary Carcinomas.. We next ... ECM Microenvironment Has Minor Effects on Average Gene Expression.. Taken together, our results suggest that the pattern of ... ECM microenvironment regulates collective migration and local dissemination in normal and malignant mammary epithelium. Kim-Vy ... 5 F and G). Thus, the ECM microenvironment had a relatively small impact on average RNA expression, despite its large effects ...
2. The Bone Marrow Microenvironment. 2.1. Cellular Compartment. 2.1.1. Bone Marrow Stromal Cells (BMSCs) in MM Progression. MM ... Bone Marrow Microenvironment in Multiple Myeloma Progression. S. Manier,1,2 A. Sacco,1 X. Leleu,2 I. M. Ghobrial,1 and A. M. ... The non-cellular compartment is composed of the extracellular matrix (ECM) and the liquid milieu including cytokines, growth ... C. M. Edwards, J. R. Edwards, S. T. Lwin et al., "Increasing wnt signaling in the bone marrow microenvironment inhibits the ...
Here we summarize our current knowledge of the roles of B cells and antibodies in the tumour microenvironment. Moreover, we ... Antibody-dependent cellular cytotoxicity mediated by cetuximab against lung cancer cell lines. Clin. Cancer Res. 13, 1552-1561 ... Wherry, E. J. & Kurachi, M. Molecular and cellular insights into T cell exhaustion. Nat. Rev. Immunol. 15, 486-499 (2015). ... B cells, plasma cells and antibody repertoires in the tumour microenvironment. *George V. Sharonov ORCID: orcid.org/0000-0001- ...
Cellular Microbiology. 9 (10): 2315-22. doi:10.1111/j.1462-5822.2007.00995.x. hdl:2027.42/73608. PMID 17587328. Chaudhari, ... "Lactate and Acidity in the Cancer Microenvironment". Annual Review of Cancer Biology. 4: 141-158. doi:10.1146/annurev-cancerbio ...
Cellular components of the tumor microenvironment. Developing tumors recruit a diverse collection of cells that make up the ... Metastatic tumor cells also recreate complex cellular microenvironments at metastatic sites. Over 120 years ago, Paget proposed ... as well as multiple cellular elements in the microenvironment, co-evolve during the process of carcinogenesis (22). Bi- ... Tumour microenvironment: TGFbeta: the molecular Jekyll and Hyde of cancer. Nat Rev Cancer. 2006;6(7):506-520. doi: 10.1038/ ...
  • Cellular microenvironments consist of a variety of cues, such as growth factors, extracellular matrices, and intercellular interactions. (jove.com)
  • Over the past years, it has become evident that cancer initiation and progression depends on several components of the tumor microenvironment, including inflammatory and immune cells, fibroblasts, endothelial cells, adipocytes, and extracellular matrix. (jcancer.org)
  • For this, conducting polyaniline (PANI) substrates were coated with collagen type 1 (Coll) alone or in association with sulfated hyaluronan (sHya) to form artificial extracellular matrix (aECM), which mimics the native microenvironment of bone tissue. (nih.gov)
  • The zone in which these cells interact with one another and the extracellular matrix (ECM) is known as the tumor microenvironment (TME). (abcam.com)
  • The BM niche is composed of a cellular compartment (stromal cells, osteoblasts, osteoclasts, endothelial cells, and immune cells) and a noncellular compartment including the extracellular matrix (ECM) and the liquid milieu (cytokines, growth factors, and chemokines). (hindawi.com)
  • The non-cellular compartment is composed of the extracellular matrix (ECM) and the liquid milieu including cytokines, growth factors, and chemokines. (hindawi.com)
  • The extracellular matrix (ECM) has a profound influences on individual cells and influences and/or controls several basic cellular processes such as adhesion, differentiation, survival, growth, and migration. (hindawi.com)
  • We focus on physical strategies (e.g., geometrical constraint, mechanical stimulation, extracellular matrix (ECM) stiffness, and topography) and biochemical approaches (e.g., genetic engineering, soluble bioactive factors, coculture, and synthetic small molecules), and highlight the three-dimensional (3D) hydrogel-based microenvironment for directed ESC differentiation. (asme.org)
  • The tumor microenvironment (TME) is the environment around a tumor, including the surrounding blood vessels, immune cells, fibroblasts, signaling molecules and the extracellular matrix (ECM). (wikipedia.org)
  • Tumors can influence the microenvironment by releasing extracellular signals, promoting tumor angiogenesis and inducing peripheral immune tolerance, while the immune cells in the microenvironment can affect the growth and evolution of cancerous cells. (wikipedia.org)
  • No matter the cause, this leaves the extracellular microenvironment acidic (pH 6.5-6.9), while the cancer cells themselves are able to remain neutral (ph 7.2-7.4). (wikipedia.org)
  • To reach this goal, we have recently acquired BIOMIMESYS ® technology , which is a unique and natural hyaluronic acid-based hydroscaffold, biofunctionalized with other extracellular matrix (ECM) components to better mimic the microenvironment of every organ. (selectscience.net)
  • We are broadly interested in manipulating the extracellular matrix (ECM) microenvironment to induce cell fate commitment or to modulate other cellular functions. (stanford.edu)
  • The extracellular matrix (ECM), one of the key components of tumor microenvironment, has a tremendous impact on cancer development and highly influences tumor cell features. (biomedcentral.com)
  • The microenvironment of tumors contains numerous cell types in addition to cancer cells, which include bone marrow-derived inflammatory cells, lymphocytes, blood vessels, fibroblastic cells, and the extracellular matrix composed of collagen and proteoglycans ( 1 , 2 ). (sciencemag.org)
  • Microenvironment extracellular matrices (ECMs) impact cell adhesion, differentiation and proliferation. (exposed-skin-care.net)
  • Solid tumors have inhibitory microenvironments that limit the efficacy of targeted therapies ( 3 ). (sciencemag.org)
  • Myeloid-derived suppressor cells (MDSCs) play a central role in maintaining the tumor microenvironment (TME) of these solid tumors by suppressing host immunity, establishing new vasculature, and remodeling tissue with tumor-supportive stromal elements ( 4 ). (sciencemag.org)
  • Therefore, we tested whether deletion of an adhesion gene could induce sustained dissemination of nontransformed cells into collagen I. We found that deletion of P-cadherin was sufficient for sustained dissemination, but exclusively into collagen I. Our data reveal that metastatic tumors preferentially disseminate in specific ECM microenvironments. (pnas.org)
  • In the tumor microenvironment, "Fas counterattack" mounted by tumors against immune cells is mediated by tumor-associated FasL. (biomedsearch.com)
  • Dr. Courtney Crane is developing novel cellular therapies for solid tumors, particularly brain cancers such as gliomas. (seattlechildrens.org)
  • This observation suggested the hypothesis that AMPK is important for the adaptive responses of energetically stressed cells in the hypoxic and glucose-deprived microenvironments present in solid tumors (e.g., reviewed in references 35 and 59 ). (asm.org)
  • Angiogenic Characteristics of Radiological Hypervascular Liver Tumors (NET, Hepatocellular Carcinoma) and Hypovascular Colorectal Liver Metastases and Their Microenvironment. (enets.org)
  • Hypothesis: Expression of angiogenic molecules of the VEGF-family and Angiopoietin/Tie-2 system in tumor and microenvironment are (1) comparable in two types of hypervascular liver tumors (hepatocellular carcinoma (HCC) and neuroendocrine liver metastases (GEP-NETM)), and (2) are lower in hypovascular colorectal liver metastases (CRLM) than in hypervascular GEP-NETM. (enets.org)
  • Understanding the cellular, molecular, and biochemical interactions of tumors within their microenvironment is essential to improving cancer diagnosis and treatment. (cancer.gov)
  • However, detailed analysis at the molecular level of 2D/3D cell cultures and tumors in vivo is still needed to elucidate cellular pathways most promising for the development of targeted therapies. (biomedcentral.com)
  • To examine the effect of the microenvironment, SRC tumors were initiated at different transplantation sites. (biomedcentral.com)
  • This report describes the first global molecular characterization of the SRC model, and it demonstrates that the tumor microenvironment can induce epigenetic alterations and changes in gene expression in the SRC tumors. (biomedcentral.com)
  • Since tumors grown at different transplantation sites were initiated from the same primary tumor, the increase in malignancy observed with the SRC tibia tumor is likely to result from the interaction between the tumor and its microenvironment. (biomedcentral.com)
  • In this study, we investigated the cell motility and the cell mitosis of proliferating human endothelial cells, critical components of the tumor microenvironment, while they are treated with PAMAM dendrimer nanoparticles using time-lapsed video microscopy. (aacrjournals.org)
  • These components of the tumor microenvironment and the neoplastic cells interact with each other providing pro and antitumor signals. (jcancer.org)
  • These cancer stem cells (CSCs) are regulated by complex interactions with the components of the tumor microenvironment - including mesenchymal stem cells, adipocytes, tumor associated fibroblasts, endothelial cells, and immune cells - through networks of cytokines and growth factors. (pubmedcentralcanada.ca)
  • Adipocytes are known to be involved in regulating the tumor microenvironment. (businesswire.com)
  • However, only few nanoparticles are capable of spreading throughout the entire tumor because of several biological barriers in the tumor microenvironment, such as the dense tumor stroma, abnormal angiogenesis, and elevated tumor interstitial fluid pressure (IFP). (thno.org)
  • In these experiments, which are a subset of all the experiments reported in the original publication, Goetz and colleagues show in a subcutaneous xenograft model that stromal caveolin-1 remodels the intratumoral microenvironment, which is correlated with increased metastasis formation. (elifesciences.org)
  • In this book, we discussed characteristics of breast cancer cell, role of microenvironment, stem cells and metastasis for this deadly cancer. (intechopen.com)
  • Because metastasis to bone often occurs after prostate cancer becomes androgen independent, we used mouse xenograft models to investigate the effect of androgens and the bone microenvironment on the nuclear translocation of ErbB3 in prostate cancer. (aacrjournals.org)
  • Studies of prostate cancer cells and stromal cells from both prostate and bone, the major site for prostate cancer metastasis, showed that cancer cells and a subset of stromal cells increased production of perlecan in response to cytokines present in the tumor microenvironment. (rice.edu)
  • Because galectin-3 is associated with various tissue remodeling events, this galectin-3-induced epithelial-stromal paracrine network may also be relevant in other pathophysiological contexts in which microenvironment remodeling plays a critical role, such as cancer cell invasion and metastasis. (sciencemag.org)
  • Here we present a novel and highly promising source of biomarkers, the tumor interstitial fluid (TIF) that perfuses the breast tumor microenvironment. (mcponline.org)
  • General culture dishes and microtiter plates, however, are not designed to enable precise physical and chemical cell manipulation at the cellular level with the range of nano- to micro-meters, which is a critical factor for cellular expansion, self-renewal, and differentiation. (jove.com)
  • Much of our understanding of the biological mechanisms that underlie cellular functions, such as migration, differentiation and force-sensing has been garnered from studying cells cultured on two-dimensional (2D) glass or plastic surfaces. (biologists.org)
  • In this context, a fundamentally different approach was adopted, and we have demonstrated the interplay of inherent substrate conductivity, defined chemical composition of cellular microenvironment, and intermittent delivery of electric pulses to drive mesenchymal stem cell differentiation toward osteogenesis. (nih.gov)
  • Although tumor heterogeneity has been long recognized, the existence of cellular hierarchies of tumor cells resulting from differentiation has only been more recently appreciated. (pubmedcentralcanada.ca)
  • In contrast, differentiation of ESCs in vitro, affords more controlled methods to present morphogenic cues in the stem cell microenvironment and directly assess differentiated cell phenotypes. (wiley.com)
  • 2010). HIF-1a regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment. (springer.com)
  • Engineering and biomaterial-based approaches to recreate a biomimetic cellular microenvironment provide valuable strategies for directing ESCs differentiation to specific lineages in vitro. (asme.org)
  • The ability to modulate stem cell differentiation in a three dimensional (3D) microenvironment for bone tissue engineering in the absence of exogenous pharmaceutical agents such as bone morphogenic protein (BMP-2) remains a challenge. (rsc.org)
  • She is particularly interested in how the microenvironment controls cell proliferation, differentiation and inflammation. (uci.edu)
  • Hypoxia-inducible factor (HIF) 1α was found to be primarily responsible for the observed effects of the tumor microenvironment on MDSC differentiation and function. (rupress.org)
  • Thus, hypoxia via HIF-1α dramatically alters the function of MDSC in the tumor microenvironment and redirects their differentiation toward tumor-associated macrophages, hence providing a mechanistic link between different myeloid suppressive cells in the tumor microenvironment. (rupress.org)
  • ECM affects vital cellular functions such as cell differentiation, migration, survival and proliferation. (biomedcentral.com)
  • Caspase 3 is responsible for cellular differentiation, although it is unclear how this kind of protein can promote the cell apoptosis. (wikipedia.org)
  • Targeting of p21 promoter is responsible for inducing cell differentiation, which is promoted by modifying the DNA nuclear microenvironment. (wikipedia.org)
  • As is the case with their normal counterparts, CSCs are regulated by intrinsic signals as well as extrinsic signals originating in the tumor microenvironment ( 13 ), and epigenetic as well as genetic changes which occur during carcinogenesis modulate these regulatory signals ( 14 , 15 ). (pubmedcentralcanada.ca)
  • NFkappaB, a key link between inflammation and cancer, regulates dual activities of death ligands, depending on molecular signals in the tumor microenvironment. (biomedsearch.com)
  • For over a century, two-dimensional (2D) cell cultures were used to study cellular response to biophysical and biochemical cues. (news-medical.net)
  • This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling. (jove.com)
  • These niches provide instructive cues to maintain GSCs and induce cellular plasticity towards a stem-like phenotype. (nih.gov)
  • At the fundamental level, Liu studies how the microenvironment influences cellular behavior, particularly how cells adhere to the underlying substrate and neighboring cells, and the mechanical forces present, by looking at physical cues in the microenvironment. (uci.edu)
  • His research focused on understanding the physical and chemical cues of the cellular microenvironment and their impact on stem cell function. (selectbiosciences.com)
  • Today there is evidence indicating that tumor growth and progression is dependent on the malignant potential of the tumor cells as well as on the multidirectional interactions of local factors produced by all the cell types-tumor, stroma, endothelial cells, and immune and inflammatory cells-present in the local microenvironment (1-9 and references therein). (mcponline.org)
  • Although the precise cause of MPNST remains unknown, studies suggest that dysregulation of Schwann cells, mediated by the microenvironment, plays a key role in tumor progression. (frontiersin.org)
  • This study aimed to further characterize the role of local microenvironment on tumor progression, with an emphasis on identifying factors within tumor suppressive environments that have potential for therapeutic application. (frontiersin.org)
  • In this paper we discuss how the interaction between the malignant plasma cell and the BM microenvironment allowed myeloma progression through cell homing and the new concept of premetastatic niche. (hindawi.com)
  • The importance of the tumor microenvironment in cancer progression is well established. (elifesciences.org)
  • It is a disease of various systems and components that interact at both a molecular and cellular level to lead to initiation and progression of the disease. (nih.gov)
  • Growing evidence suggests that extratumoral microenvironment may play a role in cancer progression. (biomedcentral.com)
  • For any type of cancer, the microenvironment is critical for the metastatic progression of cancer cells to distant sites ( 13 ). (aacrjournals.org)
  • The propensity of prostate cancer to metastasize to bone suggests that interactions between the metastatic prostate cancer cells and the tumor microenvironment are important in the progression of prostate cancer ( 15 - 18 ). (aacrjournals.org)
  • Tumor and microenvironment are important for tumor progression. (enets.org)
  • The aim of this Request for Applications (RFA) is to foster application of recent advances in molecular and cellular biology, using appropriate model systems, to study the effects of tumor cell-stroma interactions relevant to tumor development and progression in human breast or prostatic tissues. (nih.gov)
  • However, in the context of cancer, these relationships within the TME can be hijacked by factors expressed by tumor cells or by alternative cell types in the microenvironment, such as blood or lymphatic endothelial cells, adipocytes and fibroblasts. (frontiersin.org)
  • The cellular compartment is composed of hematopoietic cells and nonhematopoietic cells including fibroblasts/BMSC, endothelial cells (ECs), osteoclasts, and osteoblasts. (hindawi.com)
  • Endothelial outgrowth from aligned nanofibrillar scaffolds.HumanECsseededon fibronectin-precoated control or aligned scaffold were encapsulated into a 3D hydrogel for tracking cellular outgrowth. (stanford.edu)
  • Specifically, the liver microenvironment induces metabolic adaptations via upregulating expression of endothelial lipase in leukemia cells, which not only stimulates tumor cell proliferation through polyunsaturated fatty acid-mediated pathways, but also promotes survival by stabilizing antiapoptotic proteins. (aacrjournals.org)
  • In cancer biology, the stroma is defined as the nonmalignant cells which are present in the tumor microenvironment. (wikipedia.org)
  • accordingly, we hypothesize that specific targeting of the tumor microenvironment may constitute an alternative or additional strategy to conventional tumor-directed chemotherapy. (bloodjournal.org)
  • In this review, we expatiate on various responsive modes triggered by the tumor microenvironment stimuli, introduce recent advances in multistage nanoparticle systems, especially the multi-stimuli responsive delivery systems, and discuss their functions, effects, and prospects. (thno.org)
  • This dataset describes a novel approach for identifying biologically relevant therapeutic targets, such as CNTF, and highlights the complex relationship that tumor cells have with their local microenvironment. (frontiersin.org)
  • A common form of communication between these cells involves the production and secretion of paracrine signals, such as growth factors and cytokines, that initiate changes in cellular behavior and transform the local microenvironment. (sciencemag.org)
  • Here we summarize our current knowledge of the roles of B cells and antibodies in the tumour microenvironment. (nature.com)
  • In addition, tumour-associated macrophages and myeloid-derived suppressor cells are found to be highly enriched in the tumour microenvironment. (bmj.com)
  • Here, we review the evidence that stromal cells of the tumor microenvironment mediate this restriction by excluding T cells from the vicinity of cancer cells. (sciencemag.org)
  • In recent years, an increasing number of studies have been conducted to reconstruct cellular microenvironments in vitro 7 , 8 . (jove.com)
  • Using in vivo and in vitro models of AML, we show that bone marrow adipocytes from the tumor microenvironment support the survival and proliferation of malignant cells from patients with AML. (bloodjournal.org)
  • Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). (scielo.org.ar)
  • Thus, we demonstrate, for the first time, the presence of stem cells in hfSFs, and that modulation of the in vitro microenvironment can enhance hfSDSC chondrogenesis. (ebscohost.com)
  • The biotechnological start-up is focused on in vitro preclinical research and development with a specialization in cellular imaging: high-content analysis (HCA) and high-content screening (HCS). (selectscience.net)
  • In this SelectScience ® interview, Maubon provides insight into the development of in vitro models and assays to advance phenotypic screening, and the cellular imaging technology enabling her work. (selectscience.net)
  • Altogether, we develop new in vitro models and assays by taking into account the cellular microenvironment, because it plays a fundamental role in cell homeostasis and in pathological conditions. (selectscience.net)
  • We have been investigating the relationship between the activation of hypoxia-inducible factor 1 (HIF-1), the primary transcriptional regulator of the mammalian response to hypoxia, and 5′-AMP-activated protein kinase (AMPK), another regulatory system important for controlling cellular energy metabolism. (asm.org)
  • We obtained evidence that AMPK is activated in authentic hypoxic tumor microenvironments and that this activity overlaps with regions of hypoxia detected by a chemical probe. (asm.org)
  • While exploring cellular mechanisms of ATP regulation, we observed that 5′-AMP-activated protein kinase (AMPK) activity was induced in both cell types, particularly under conditions of hypoxia and glucose deprivation. (asm.org)
  • 1. To study the effect of the microenvironment on the expression of chondrogenic markers by mouse embryonic fibroblasts, when cultured in the self-assembling peptide RAD16-I. (prezi.com)
  • We demonstrate in this study that factors derived from the microenvironment, including signals from the B cell-activating factor belonging to the TNF family/BLyS survival factor, integrin-regulated homeostasis, and circulatory dynamics of B cells define distinct in vivo mechanism(s) and sensitivities of cells in anti-hCD20 mAb-directed therapies. (jimmunol.org)
  • These therapeutic mAbs neutralize pathogenic cytokines (e.g., anti-TNF mAbs for rheumatoid arthritis), inhibit cellular proliferation (e.g., herceptin for Her-2 + breast cancers), induce agonistic or inhibitory receptor activities to regulate cellular functions (e.g., anti-CD3 mAb for transplantation rejection), and eliminate malignant and pathogenic cells by targeting cell surface Ags ( 1 , 2 , 3 ). (jimmunol.org)
  • In this study, we demonstrate a greater complexity involving the circulatory dynamics of B cells and survival factors from the microenvironment as critical regulators that determine the mechanistic basis for immunodepletion through complement or the reticuloendothelial system (RES) 3 . (jimmunol.org)
  • We investigated a nano-radiomics approach (quantitative analysis of nanoparticle contrast-enhanced three-dimensional images) for detection of tumor response to cellular immunotherapy directed against myeloid-derived suppressor cells (MDSCs), a key component of TME. (sciencemag.org)
  • Cellular immunotherapies such as chimeric antigen receptor (CAR)-bearing T cells have shown efficacy in clinical trials of hematologic malignancies ( 1 ). (sciencemag.org)
  • During the remodeling of the pulmonary tissue, CD30+ ATL cells and matrix metalloproteinases can play an important role in forming tumor microenvironment. (omicsonline.org)
  • Furthermore, soluble cytokine receptors may support the clonal proliferation and invasion of ATL cells and tumor microenvironment is created in extra nodal lesions such as lung. (omicsonline.org)
  • Accordingly, sCD30 level could be applied to detection of microenvironment formation for tumor stem cells and their monitoring in ATL. (omicsonline.org)
  • The striking disparity between traditional monolayer culture and the in vivo scenario has been a double-edged sword: the simplicity of 2D culture has enabled reductionist approaches to understanding individual cellular phenomena but these findings have come with the caveat that the 2D model might not faithfully capture the physiological behavior of cells in vivo. (biologists.org)
  • To develop a robust support system, malignant cells must recruit non-malignant cells from within the stromal tissue, the cellular milieu in which a tumor develops, that would otherwise act to inhibit cancer development. (abcam.com)
  • In addition to these oncogenic events, the tumor cells are strongly dependent on the bone marrow microenvironment [ 6 ]. (hindawi.com)
  • However the new host microenvironment is not well adapted to the cancer cells that metastasized into it, leading to the new concept of premetastatic niche [ 8 ]. (hindawi.com)
  • Although much research has focused on the genetic abnormalities that initiate and drive cancer, there is now overwhelming evidence that the behavior of tumorigenic cells is also highly influenced by their microenvironment. (pubmedcentralcanada.ca)
  • Analogous to the regulation of normal stem cells by their "niche," CSCs are regulated by, and in turn regulate, cells within the tumor microenvironment. (pubmedcentralcanada.ca)
  • Indeed, these therapeutic approaches can be further revisited with the advent of nanotechnology that can target the key cellular components of TME and tumor cells more precisely. (springer.com)
  • Exosomes are involved in this inter-cellular communication by shuttling messages between NBL cells and monocytes, neighboring white blood cells that are part of the body's innate immune system. (eurekalert.org)
  • The unique tumor microenvironment of endometriosis is composed of epithelial, stromal, and immune cells, which adapt to survive in hypoxic conditions with high levels of iron, estrogen, and inflammatory cytokines and chemokines. (mdpi.com)
  • Similar to their healthy counterpart, malignant hematopoietic stem cells in myeloid malignancies, such as myeloproliferative neoplasms, myelodysplastic syndromes, and acute myeloid leukemia, reside in a highly complex and dynamic cellular microenvironment in the bone marrow. (nih.gov)
  • To use isolated cells or cells substitutes as cellular replacement parts. (prezi.com)
  • The penetration of anticancer drugs through tumor tissue as a function of cellular adhesion and packing density of tumor cells. (springer.com)
  • Dr. Crane's laboratory engineers immune cells to be tissue-resident, persistent, nonproliferating sources of proteins that alter the tumor microenvironment to promote antitumor immunity. (seattlechildrens.org)
  • These vectors sense the microenvironment and express the genes they carry only when cells encounter certain conditions. (seattlechildrens.org)
  • Dr. Crane's use of immune-stimulating proteins, locally delivered by engineered immune cells, may synergize with other types of immune-based therapies to overcome immune suppression in the solid tumor microenvironment, including adoptive CAR T cells, checkpoint blockades, and small molecules or peptides. (seattlechildrens.org)
  • Because of this, we speculated that dental pulp cells would be capable of expressing dentin-specific genes and proteins inside the diffusion chamber in the host microenvironment. (scielo.org.ar)
  • Thus, to clarify the influence of the microenvironment in protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1cells (bone marrow stromal cell line) were seeded either in cell culture or in intra-abdominal diffusion chambers. (scielo.org.ar)
  • Targeted delivery to single cells in precisely controlled microenvironments. (sigmaaldrich.com)
  • These interacting systems include interactions between genes in the cancer cells, between signal transduction pathways within a cancer cell, between cells in the tumor, between the tumor and its microenvironment, and between the individual and the macro-environment. (nih.gov)
  • These findings suggest that the bone microenvironment and androgen status influence the subcellular localization of ErbB-3 in prostate cancer cells. (aacrjournals.org)
  • Our findings suggest that the cellular localization of ErbB-3 in prostate cancer cells is a dynamic process that can be influenced by the tumor microenvironment and androgen status. (aacrjournals.org)
  • Duvelisib complements Verastem's oncology pipeline by augmenting our strategic focus of developing small molecule agents that target malignant cells both directly and through modulation of the tumor microenvironment. (corporate-ir.net)
  • I am really convinced that to find effective drugs, we must reproduce the pathology as a whole, taking into account many (if not all) the cells of the diseased organ and their microenvironment. (selectscience.net)
  • Research in this area seeks to understand the role of tumor cells and the tumor microenvironment in driving cancer processes. (cancer.gov)
  • Using what is observed about how cells respond to their microenvironment, Liu is working to develop materials and scaffolds that have a controlled host response after implantation into the body. (uci.edu)
  • Little is known of the contribution of this mosaic of cells to the malignant process at the cellular or molecular level. (nih.gov)
  • these non-transformed cells forming the microenvironment in which tumour cell growth is contained and supported. (biomedcentral.com)
  • The tumor microenvironment caused rapid and dramatic up-regulation of arginase I and inducible nitric oxide synthase in MDSC, which was accompanied by down-regulation of nicotinamide adenine dinucleotide phosphate-oxidase and reactive oxygen species in these cells. (rupress.org)
  • Due to the disseminated nature of leukemia, malignant cells are exposed to many different tissue microenvironments, including a variety of extramedullary sites. (aacrjournals.org)
  • Together, these studies demonstrate a unique role for liver in modulating the pathogenesis of leukemic disease and suggest that the hepatic microenvironment may protect leukemia cells from chemotherapeutic challenge. (aacrjournals.org)
  • The studies presented herein demonstrate that the liver provides a microenvironment in which leukemia cells acquire unique metabolic properties. (aacrjournals.org)
  • Global gene and miRNA expression analysis in LLC1 cells under ECM microenvironment indicated altered immune response, adhesion and MAP kinase pathways. (biomedcentral.com)
  • Both epithelial cells and fibroblasts produce large amounts of matrix-degrading enzymes after the establishment of heterotypic cellular interactions, but the precise paracrine mechanisms responsible for the stimulation of these proteases remain mostly uncharacterized. (sciencemag.org)
  • The importance of a stromal microenvironment, especially one that has characteristics of a "wound" or regenerating tissue, has been recognized for at least a century ( 3 ), but its possible role in blunting an immune attack of cancer cells awaited the discovery of adaptive cellular immunity. (sciencemag.org)
  • Immunoediting, or the elimination of immunogenic cancer cells ( 7 ), could be excluded, which left the possibility of immune suppression by the tumor microenvironment (TME). (sciencemag.org)
  • Although the microenvironment has recently been demonstrated to play critical roles in B cell homeostasis, its contribution to immunotherapy is unknown. (jimmunol.org)
  • CytoReason's unique machine learning platform uncovers new cellular players in tumor microenvironment - new potential directions for improved immunotherapy. (businesswire.com)
  • TEL AVIV, Israel--( BUSINESS WIRE )--New findings presented today by CytoReason reveals possible new cellular players in the tumor microenvironment that could impact the treatment process for the most in-need patients - those who have already failed to respond to ipilimumab (anti-CTLA4) immunotherapy. (businesswire.com)
  • Our study shows that TME-directed cellular immunotherapy causes subtle changes not effectively gauged by conventional imaging metrics but revealed by nano-radiomics. (sciencemag.org)
  • Using cell cultures, scientists are able to manipulate the cellular microenvironment in order to simulate physiological conditions in vivo . (news-medical.net)
  • We created GFP-tagged adult induced tumorigenic Schwann cell lines (iSCs) and transplanted them into various in vivo microenvironments. (frontiersin.org)
  • Many studies indicate that this lineage commitment is precisely controlled by the ESC microenvironment in vivo. (asme.org)
  • Using bioluminescent and fluorescent in vivo imaging tools, Liu examines the biomaterials for compatibility, and is also developing better tools to visualize specific cellular processes that are involved in the function and integration of these biocompatible materials. (uci.edu)
  • The cellular heterogeneity of glioblastomas is manifest in genetic and epigenetic variation with both stochastic and hierarchical models informing cellular phenotypes. (nih.gov)
  • The programme explores the cellular, genetic and epigenetic basis of cancer, and also covers the scientific and clinical challenges pertinent to the management of site-specific cancers. (tcd.ie)
  • 6. Predicting and optimizing cellular responses to therapeutic strategies that target the TME. (frontiersin.org)
  • These results indicate that CAF-derived LOXL2 is an important mediator of intercellular communication within the prostate tumor microenvironment and is a potential therapeutic target. (mcponline.org)
  • The interaction between CSCs and their microenvironment provides new targets for therapeutic intervention. (pubmedcentralcanada.ca)
  • The microenvironment in human myeloid malignancies: emerging concepts and therapeutic implications. (nih.gov)
  • Our results indicate that modifying the tumor microenvironment using intratumoral Ad-Flt3L/Ad-TK-mediated gene therapy potentiates therapeutic efficacy and antitumor immunity induced by DC vaccination. (aacrjournals.org)
  • We tested the hypothesis that modifying the tumor microenvironment using in situ Ad-Flt3L/Ad-TK-mediated gene therapy, which involves expression of fms-like tyrosine kinase 3 ligand (Flt3L) and thymidine kinase (TK) within the tumor mass with concomitant release of tumor antigens and high-mobility group B1 protein (HMGB1), would potentiate the therapeutic efficacy and antitumor immunity induced by DC vaccination. (aacrjournals.org)
  • Wu T, Dai Y. Tumor microenvironment and therapeutic response. (springer.com)
  • Other areas of research in the Crane lab include capitalizing on new knowledge about tumor microenvironments to identify biomarkers and therapeutic targets. (seattlechildrens.org)
  • Substantial advances have been made in understanding the biology of multiple myeloma (MM) through the study of the bone marrow (BM) microenvironment. (hindawi.com)
  • Indeed significant changes occur in the microenvironment even before the first tumor cell homes to the bone marrow, as already described in solid tumor models [ 8 ]. (hindawi.com)
  • A large number of studies have extensively addressed the composition of the bone marrow niche in mouse models, as well as the cellular and molecular communication modalities at play under both normal and pathogenic situations. (nih.gov)
  • We thus assessed the effect of androgens and the bone microenvironment on the nuclear translocation of ErbB-3 by using xenograft tumor models generated from bone-derived prostate cancer cell lines, MDA PCa 2b, and PC-3. (aacrjournals.org)
  • while a numbers of key growth factors that regulates hematopoiesis have been identified and some of them are currently used for clinical purpose, how bone marrow microenvironment that presents those signals to the hematopoietic progenitors is constructed remains totally obscure. (nii.ac.jp)
  • These findings imply that HIF-1 and AMPK are components of a concerted cellular response to maintain energy homeostasis in low-oxygen or ischemic-tissue microenvironments. (asm.org)
  • This new MR imaging framework-which we term "magnetic resonance microdynamic imaging (MRMI)"-permits the simultaneous noninvasive and model-free quantification of multiple subcellular, cellular, and interstitial tissue microenvironments within a voxel. (ovid.com)
  • Tissue microenvironments images correlated with immunohistochemistry findings. (ovid.com)
  • There remains a need for new oral medicines, and the targeted inhibition of PI3K-delta and PI3K-gamma brings a unique approach designed to address both the malignant B cell and its supportive microenvironment. (corporate-ir.net)
  • The tumor microenvironment is composed of a variety of different cell types that work in concert to support or limit the growing tumor. (frontiersin.org)
  • Understanding the network of communication between multiple cell types within the microenvironment is key to understanding how to block immune evasion and to stimulate anti-tumor immune responses. (frontiersin.org)
  • The Sensing Cell Culture Flask (SCCF) is a cell culture monitoring system accessing the cellular microenvironment in 2D cell culture using electrochemical microsensors. (mdpi.com)
  • Kieninger J, Tamari Y, Enderle B, Jobst G, Sandvik JA, Pettersen EO, Urban GA. Sensor Access to the Cellular Microenvironment Using the Sensing Cell Culture Flask. (mdpi.com)
  • The book provides critical insight to how the tumor microenvironment affects tumor metabolism, cell stemness, cell viability, genomic instability and more. (springer.com)
  • To address this drawback, studies have investigated the roles of cellular microenvironments in regulating cell-fate decisions and cell functions 4 . (jove.com)
  • This approach provided for the first time a snapshot of the protein components of the TIF, which we show consists of more than one thousand proteins-either secreted, shed by membrane vesicles, or externalized due to cell death-produced by the complex network of cell types that make up the tumor microenvironment. (mcponline.org)
  • With the advent of enabling technologies within proteomics, it is now feasible to undertake a systematic characterization of the proteins that are released to the interstitial space by all the cell types resident in the tumor microenvironment. (mcponline.org)
  • However, approximately 90% of the specific pattern of cellular gene expression signature is driven by the cell composition of the sample. (businesswire.com)
  • In this study, using a single published data set*, CytoReason was able to apply its knowledge base and technologies to rebuild cellular composition and cell specific expression. (businesswire.com)
  • This enabled CytoReason to undertake a cell level analysis, uncovering hidden cellular activity that was mapped back to specific genes that can be shown to emerge only when therapy is showing and effect. (businesswire.com)
  • Immunotherapies, including cell-based therapies, targeting the tumor microenvironment (TME) result in variable and delayed responses. (sciencemag.org)
  • Pornkuna R, Takemoto S (2014) A Lack of Cellular Senescence, Formation of Microenvironment, and Role of Soluble Cd30 in Development of Adult T-Cell Leukemia/Lymphoma. (omicsonline.org)
  • Thus, the focus of this Commentary will be on identifying and describing the fundamental features of 3D cell culture systems that influence cell structure, adhesion, mechanotransduction and signaling in response to soluble factors, which - in turn - regulate overall cellular function in ways that depart dramatically from traditional 2D culture formats. (biologists.org)
  • Treatment with CaMKK2 inhibitors blocks tumor growth in a CD8 + T cell-dependent manner, and facilitates a favorable reprogramming of the immune cell microenvironment. (nature.com)
  • Wherry, E. J. & Kurachi, M. Molecular and cellular insights into T cell exhaustion. (nature.com)
  • Notably, GL261 neurospheres produced 3.7-fold more TGF-β per cell compared with conventional culture, suggesting that TGF-β production by GIC promotes effective DNA damage response and self-renewal, which creates microenvironment-mediated resistance. (aacrjournals.org)
  • It turns out this is because MiR155 doesn't derive from the cancer cell line, but from the tumor microenvironment, and is secreted by the exosome back to the cancer cell where it silences an important gene - TERF1. (eurekalert.org)
  • Molecular connections between cancer cell metabolism and the tumor microenvironment. (springer.com)
  • It is for this reason that a basic understanding of critical cellular processes, such as cell cycle, DNA repair, apoptosis, transcription, cell migration, and matrix structure, is so critical to our understanding and treatment of cancer. (nih.gov)
  • Unsupervised clustering identified two distinct gene expression subgroups that differed in expression of genes involved in activation of fibrosis, cellular movement, cell adhesion and cell-cell contact. (biomedcentral.com)
  • We evaluated the prognostic relevance of extratumoral subtype (comparing the Active group, defined by high expression of fibrosis and cellular movement genes, to the Inactive group, defined by high expression of claudins and other cellular adhesion and cell-cell contact genes) using clinical data. (biomedcentral.com)
  • As a result of these beneficial changes within the tumor microenvironment, we observed a striking enhancement by duvelisib of the anti-tumor efficacy of immune checkpoint or co-stimulatory antibodies in this preclinical B cell lymphoma model. (corporate-ir.net)
  • Using customizable ECM microenvironments, we are interested in studying mechanotransduction pathways that induce cell fate commitment. (stanford.edu)
  • By gaining fundamental insights in the role of ECM-mediated mechanotransduction pathways on cell fate commitment, we will engineer three-dimensional vascular conduits, cardiac patches, and skeletal muscle grafts with physiologically relevant cellular and ECM compositions. (stanford.edu)
  • The rate of unsuccessful clinical trials, based on cell culture research models lacking the ECM microenvironment, indicates the need for alternative models and determines the shift to three-dimensional (3D) laminin rich ECM models, better simulating tissue organization. (biomedcentral.com)
  • Tumor microenvironment (TME) was categorized into three classes by using tissue single cell analysis and multiplex immunohistochemistry (IHC) of primary human cancer tissues. (nii.ac.jp)
  • He is currently developing methods to enable single cell manipulation via sub-cellular scaled features of various biomolecules. (selectbiosciences.com)
  • Secretome alterations are often associated with atypical cellular phenotypes that are indicative of diseases such as cancer. (jcancer.org)
  • CytoReason is unique in that our disease models are specifically designed on a cellular level - replicating biology to crack key biological challenges, while learning from every data set", said David Harel, CEO, CytoReason. (businesswire.com)
  • Important factors in this improved prognosis include discovery at early age and early stage disease in women with endometriosis at time of ovarian cancer staging [ 21 , 22 , 23 , 24 ], but may also represent the unique biology from the endometriotic tumor microenvironment. (mdpi.com)
  • With this Funding Opportunity Announcement (FOA), the National Cancer Institute (NCI) encourages new collaborative projects between investigators associated with the Integrative Cancer Biology Program (ICBP) or Tumor Microenvironment Network (TMEN) and researchers who are not involved with the program with which they propose to collaborate. (nih.gov)
  • This FOA is designed to facilitate new projects in integrative cancer biology and/or tumor microenvironment research and to extend current research conducted in the ICBP and TMEN programs through collaborations with a broader research community. (nih.gov)
  • The purpose of this Funding Opportunity Announcement (FOA) is to encourage new research into the complexity of cancer by fostering new interactions with two existing and related programs within the NCI: the Integrative Cancer Biology Program (ICBP) and/or the Tumor Microenvironment Network (TMEN). (nih.gov)
  • In particular, the proposed projects are expected to bring new expertise, methods, ideas, and resources to the fields of integrative cancer biology and tumor microenvironment research. (nih.gov)
  • In Genome Biology this week: features affecting gut microbiome and parasite patterns, cellular interactions in lung tumor microenvironment, and more. (genomeweb.com)
  • This mechanism of paracrine control has broad importance for our understanding of how the proteolytic microenvironment is modified in epithelial-stromal interactions. (sciencemag.org)
  • These results indicates that KL-expression in hematopoietic microenvironment is mediated by yet unknown cis-regulatory elements of KL-gene. (nii.ac.jp)
  • Bioinspired Collagen/Glycosaminoglycan-Based Cellular Microenvironments for Tuning Osteoclastogenesis. (nih.gov)
  • In multi-GAG microenvironments, atomic force microscopy revealed intermediate collagen fibril structures with thin fibrils and microfibrils. (nih.gov)
  • We used basement membrane gels to model the normal microenvironment and collagen I to model the stromal ECM. (pnas.org)
  • We also assess another critical component of the microenvironment, the "irritability" or inflammability of nanoparticles toward human lung macrophages using rocket immuno-electrophoresis, a powerful and quantitative immuno-analytical technique consists of a two-dimensional immunoelectrophoresis with a combination of conventional electrophoretic separation and electro-immunodiffusion. (aacrjournals.org)
  • Also, a balance needs to be found between the stability of the nanoparticles during the transport in blood circulation and the ideal tumor cellular uptake after extravasation into tumor tissue. (thno.org)
  • Biopsies taken from individual tumours exhibit extensive differences in their cellular composition due to the inherent heterogeneity of cancers and vagaries of sample collection. (biomedcentral.com)
  • Cellular localization was analyzed by immunohistochemistry. (enets.org)
  • Duvelisib is well aligned with Verastem's strategic focus of developing novel anti-cancer therapeutics that modulate the tumor microenvironment. (businesswire.com)
  • It was implied that aberrant cellular proteins such as p53, JAK-STAT and Bcl-xL were associated with the development of ATL. (omicsonline.org)
  • Exosomes are vesicles, or "envelopes," that deliver their cargo of microRNA (miRNA) - small molecules that are not translated into working proteins, but may regulate basic cellular processes. (eurekalert.org)
  • More targeted delivery of microenvironment-altering proteins could reduce treatment toxicity and side effects. (seattlechildrens.org)
  • 2015). Cancer prevention and therapy through the modulation of the tumor microenvironment. (springer.com)
  • After traumatic injuries to the nervous system, regrowing axons encounter a complex microenvironment where mechanisms that promote regeneration compete with inhibitory processes. (hindawi.com)
  • One major component of the tumor microenvironment is the immune system which has spawned a new and hopeful generation of cancer treatment. (frontiersin.org)
  • At the core of its capabilities is a unique and singular focus on re-defining understanding of the immune system at a cellular level. (businesswire.com)
  • As we move towards more-physiological culture systems for studying fundamental cellular processes, it is crucial to define exactly which factors are operative in 3D microenvironments. (biologists.org)
  • Crucial to these processes is the production of matrix metalloproteinases (MMPs) that modify the microenvironment. (sciencemag.org)
  • We hypothesized that gene expression subtypes of breast cancer microenvironment can be defined and that these microenvironment subtypes have clinical relevance. (biomedcentral.com)
  • The Active subtype was also associated with expression of TGF-β induced fibroblast activation signatures, but there was no significant association between Active/Inactive microenvironment and desmoid type fibrosis or estrogen response gene expression signatures. (biomedcentral.com)
  • These results suggested that cisplatin could deteriorate the immunosuppressive microenvironment through increasing PD-L1, CCL2, IL-1β by upregulated YAP1 expression. (visualsonics.com)
  • Therefore, the study suggested that YAP1 blockade destroyed the immunosuppressive microenvironment of cancer to improve the effect of chemotherapy in HCC. (visualsonics.com)
  • In order to mount an effective regenerative response after axonal injury, the injured neuron must be able to initiate a number of changes at the cellular level including an increase in expression of relevant genes and protein transport with the aim of forming an extruding process known as a growth cone [ 1 - 3 ]. (hindawi.com)
  • The interplay between the tumor and its microenvironment was part of Stephen Paget's 1889 "seed and soil" theory, in which he postulated that metastases of a particular type of cancer ("the seed") often metastasizes to certain sites ("the soil") based on the similarity of the original and secondary tumor sites. (wikipedia.org)
  • The cellular microenvironment and metastases. (medlineplus.gov)