Local surroundings with which cells interact by processing various chemical and physical signals, and by contributing their own effects to this environment.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
Methods for maintaining or growing CELLS in vitro.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

Screening the cellular microenvironment: a role for microfluidics. (1/313)

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Murine amniotic fluid stem cells contribute mesenchymal but not epithelial components to reconstituted mammary ducts. (2/313)

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Salivary glands of primary Sjogren's syndrome patients express factors vital for plasma cell survival. (3/313)

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Evolving homeostatic tissue using genetic algorithms. (4/313)

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Mathematical descriptions of biochemical networks: stability, stochasticity, evolution. (5/313)

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More than a feeling: discovering, understanding, and influencing mechanosensing pathways. (6/313)

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Toll-like receptors are key players in neurodegeneration. (7/313)

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Nattectin a fish C-type lectin drives Th1 responses in vivo: licenses macrophages to differentiate into cells exhibiting typical DC function. (8/313)

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TY - JOUR. T1 - Switchable substrates for analyzing and engineering cellular functions. AU - Nakanishi, Jun. PY - 2014/2/1. Y1 - 2014/2/1. N2 - Cellular activity is highly dependent on the extracellular environment, which is composed of surrounding cells and extracellular matrices. This focus review summarizes recent advances in chemically and physically engineered switchable substrates designed to control such cellular microenvironments by application of an external stimulus. Special attention is given to their molecular design, switching strategies, and representative examples for bioanalytical and biomedical applications.. AB - Cellular activity is highly dependent on the extracellular environment, which is composed of surrounding cells and extracellular matrices. This focus review summarizes recent advances in chemically and physically engineered switchable substrates designed to control such cellular microenvironments by application of an external stimulus. Special attention is given to ...
The colon cancer stem cell microenvironment holds keys to future cancer therapy.s profile, publications, research topics, and co-authors
Abstract While most of the in vitro cultures are carried out on bi-dimensional (2D) substrates, most of the in vivo extracellular matrices are threedimensional (3D). Consequently cells behave differently on 2D substrates as a way to self-adaptation to a non-physiological environment. This fact has encouraged the development of more relevant culture conditions seeking to provide more representative models for biomedicine (e.g. cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. Different 3D culture systems have been established though their variability and complexity hinder their standardisation in common cell culture procedures. So, this thesis deals with the dimensionality issue in cell/material interactions and introduces sandwich-like microenvironments as a versatile tool to study cell behaviour. Cells cultured within this system use both dorsal and ventral receptors to adhere and spread, undergoing important changes with ...
The objective of the presented research is to examine the relationship between the cellular microenvironment and biochemical response of metastatic cells. Clinically recognized as a trait of cancer progression, the cellular microenvironment can have variable and distinct mechanical properties that are processed via cellular mechanosensing, resulting in a cellular biochemical response. A range of studies investigating the interactions between the cellular micromechanical environment and the cells molecular response during disease progression have been made, yet remain absent of quantitative characterization of many of these coordinated responses. The fundamental inquiry that drives the following research attempts to elucidate how a cell perceives the physical microenvironment and converts that signal to a biochemical response. With the goal of providing insight to such responses, the presented research seeks to elucidate the following questions: (1) What are the integrated effects of ECM ...
Solid tumors are composed of heterogeneous cell populations that interact in complex networks. As is the case in developing organs, tumor cells interact with and in turn are regulated by these components in the microenvironment. Metastatic tumor cells also recreate complex cellular microenvironments at metastatic sites. More than 120 years ago, Paget proposed the seed and soil hypothesis of tumor metastasis (40). Reframed in a modern context, the seeds are the CSCs and the soil is the rich microenvironment, which is composed of diverse cell types that interact with tumor cells via cytokine networks. These networks regulate CSCs and their progeny, which form the tumor bulk. Elucidation of these pathways may provide new targets for therapeutic development. Examples of such pathways include the cytokines IL-6 and IL-8 and their receptors IL-6R and CXCR1. Blockade of these cytokine pathways reduced breast CSCs in preclinical models (33). Clinical trials using IL-6-blocking antibodies have been ...
Several reports have pointed out that the ECM inhibits regeneration and suppresses plasticity in the adult CNS (Berardi et al., 2004; Fitch and Silver, 2008; Gervasi et al., 2008). By comparison, the role of the ECM in the stem and precursor cell microenvironment remains obscure, although the cellular microenvironment is crucial for stem cell maintenance and self-renewal (Scadden, 2006). Focusing on OPCs as a model, we reveal an inhibitory function of the ECM glycoprotein Tnc on differentiation and disentangle molecular details of ECM pathways designed to suppress the differentiation of a committed neural precursor cell. We show that Tnc suppresses the expression of MBP in oligodendrocytes both in vitro and in vivo during postnatal forebrain development, confirming its biological significance. The underlying signaling cascade requires the cell surface receptor Cntn1 and the associated Fyn kinase, and involves the regulation of the signaling adaptor and splice regulator Sam68. We further document ...
TY - JOUR. T1 - Association of RB/p16-pathway perturbations with DCIS recurrence. T2 - Dependence on tumor versus tissue microenvironment. AU - Witkiewicz, Agnieszka K.. AU - Rivadeneira, Dayana B.. AU - Ertel, Adam. AU - Kline, Jessica. AU - Hyslop, Terry. AU - Schwartz, Gordon F.. AU - Fortina, Paolo. AU - Knudsen, Erik S.. PY - 2011/9/1. Y1 - 2011/9/1. N2 - The prevalence of ductal carcinoma in situ (DCIS) diagnoses has significantly increased as a result of active radiographic screening. Surgical resection and hormone and radiation therapies are effective treatments, but not all DCIS will progress to invasive breast cancer. Therefore, markers are needed to define tumors at low risk of recurrence and progression that can be treated by surgery alone rather than by adjuvant therapies. Initial analyses indicate that retinoblastoma (RB)-pathway perturbations occur at high frequency in DCIS and mirror the molecular alterations observed in invasive breast cancer. Particularly, the elevated ...
Participate in the open-access Research topic entitled The Adipose Tissue Microenvironment in Cancer: Molecular Mechanisms and Targets for Treatment
Evidence shows that It is only when the tissue microenvironment is degraded that cells with cancer-causing mutations find themselves most fit and suddenly able to out-compete healthy cells and so establish themselves in the landscape of the body.
Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression ...
Thanks to the co-financing of S. Spicuglia (U1090) and a mutual request of the units to the region (U1067-U1090-U1097), the common cell culture platform of the building has recently acquired: a sorter BD FACSMelody: 3 lasers, 9 colors and single-cell module a BD LSRFortessa X20 cell analyser : 3 lasers, 14 colors
Today is 04/02/10 and Id mentioned that I would follow up after i perm my hair post BKT treatment. So to recap, Id got the BKT at a high-end dept.
The molecular mechanisms that drive EMT are complex and involve many more biochemical components. These have been reviewed extensively in [10]. In addition to the biochemical signals, mechanical signals, such as the rigidity of the cells microenvironment, also play roles in the regulation of EMT. Lee et al. observed that mammary epithelial cells underwent EMT when placed on hard substrates and treated with matrix metalloproteinase-3 (MMP3). This was not observed in cells grown on soft substrates [13]. Furthermore, treatment with MMP3, caused cells on both substrates to increase their expression of Rac1b. However, only those cells on the hard substrate produced reactive oxygen species (ROS) [13] which is downstream of MMP3 and Rac1b [14]. Interestingly, in cells on hard substrates, Rac1b is recruited to the cell membrane through integrin clustering. This allows Rac1b to assemble with NADPH oxidase and contribute to ROS production. It was determined therefore, that cells growing on rigid ...
J. S. Miller and West, J. L., Biomimetic hydrogels to support and guide tissue formation, in Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications, A. Khademhosseini, Borenstein, J., Toner, M., and Takayama, S. 2008, pp. 101-120. ...
This page of BLACKROCK INCOME CF (BKT) displays a brief signal update and a market outlook, as well as a candlestick chart showing the last detected pattern.
The magic to killing cancer cells might not be in the bullet, but in the gunpowder that accompanies it, according to researchers at the University of Pittsburgh Cancer Institute (UPCI), partner with UPMC CancerCenter.
Living systems are made of complex architectural organization of various cell types in defined microenvironments. The intricate interactions between different cell types control the specific functions of the associated tissues, such as the functions of native liver and cardiac tissues, metastasis and invasion of tumors, and embryonic development.
TY - JOUR. T1 - Biochemical characterization of nuclear receptors for vitamin D3 and glucocorticoids in prostate stroma cell microenvironment. AU - Hidalgo, Alejandro A.. AU - Montecinos, Viviana P.. AU - Paredes, Roberto. AU - Godoy, Alejandro S.. AU - McNerney, Eileen M.. AU - Tovar, Heribelt. AU - Pantoja, Diego. AU - Johnson, Candace. AU - Trump, Donald. AU - Onate, Sergio A.. N1 - Funding Information: This study was supported by the Fondecyt (1080261), American Cancer Society (RGS-012301-TBE), and the Concern Foundation to Sergio A. Onate. The authors would like to acknowledge the support from the Translational Research Tissue Resource and the Pathology core facilities at Roswell Park Cancer Institute for providing prostate tissue from the organ donor program.. PY - 2011/8/19. Y1 - 2011/8/19. N2 - The disruption of stromal cell signals in prostate tissue microenvironment influences the development of prostate cancer to androgen independence. 1α,25-Dihydroxyvitamin D3 (1,25D3) and ...
Elasticity of cellular microenvironments strongly influences cell motility, phagocytosis, growth and differentiation. Currently, the relationship between the cell behaviour and matrix stiffness is being studied for cells seeded on planar substrates, however in three-dimensional (3D) microenvironments cells may experience mechanical signalling that is distinct from that on a two-dimensional matrix. We report a microfluidic approach for high-throughput generation of 3D microenvironments with different elasticity for studies of cell fate. The generation of agarose microgels with different elastic moduli was achieved by (i) introducing into a microfluidic droplet generator two streams of agarose solutions, one with a high concentration of agarose and the other one with a low concentration of agarose, at varying relative volumetric flow rate ratios of the two streams, and (ii) on-chip gelation of the precursor droplets. At 37 °C, the method enabled a ~35-fold variation of the shear elastic modulus ...
Injury-induced stem-cell niche are defined as cellular microenvironments that are generated during tissue injury, these environments are triggered by injury and the local responses of support cells, and enable the possibility of repair by endogenous or transplanted neural stem cells. These environments have been demonstrated in several injury models, most notable in the CNS. The term was coined by Jaime Imitola and Evan Y. Snyder when they demonstrated that astrocytes and endothelial cells during stroke are able to create a permissive environment for neural regeneration,[1] that is most striking for exogenous transplanted neural stem cells. Previous work by the Snyder Laboratory have shown that the interactions between NSCs and local cells is reciprocal, underlying a bystander beneficial effect of neural stem cells without neural differentiation, once thought to be the only mechanism for therapeutical benefit of stem cells in CNS injury.[2]. More recently these findings have been reproduced and ...
It isnt about who discovers the remedy first. Its about getting a cure - fast... BCBC awards $5 million for cell therapy analysis in type 1 diabetes Maike Sander, MD, associate professor of pediatrics and cellular & molecular medicine at the University of California, NORTH PARK School of Medication has been awarded nearly $5 million by the Beta Cell Biology Consortium to business lead an interdisciplinary group in cell therapy analysis for type 1 diabetes. Sander will business lead a united group of domestic and worldwide collaborators, with the purpose of generating substitute insulin-making beta cells from patient-derived induced pluripotent stem cells. The $4,950,000 grant is distributed to Karl Willert, PhD, director of the UCSD Human being Stem Cell Core Service, wholl apply a screening system for cellular microenvironments to beta cell differentiation Additional individuals consist of labs at the University of Pennsylvania and in Barcelona, Spain, in addition to a San Diego-structured ...
2509 Nitric Oxide (NO•), an important signaling molecule and a component of inflammatory response, is also involved in tumorigenesis. However, the quantity of NO• and the nature of the cellular microenvironment influences the role of NO• in tumor development. We used a genetic strategy to test the hypothesis that an inflammatory microenvironment with an enhanced level of NO• accelerates spontaneous tumor development. C. parvum-induced inflammation and increased nitric oxide synthase-2 (NOS2) expression which coincided with accelerated spontaneous tumor development, mostly lymphomas, in p53-/-NOS2+/+ C57BL6 mice as compared to the controls (p=0.001). However, p53-/-NOS2-/- mice did not show any difference in tumor latency between C. parvum-treated and control groups. C. parvum-treated p53-/-NOS2+/+ mice showed that tumor development was preceded by a higher expression of NOS2 and phosphorylated Akt-Ser 473 (pAkt-Ser473) in spleen, increased cell proliferation measured by Ki-67 ...
Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.. ...
The cellular microenvironment plays a crucial role in leading differentiation and proliferation of stem cells. hMSCs within GFOGER-modified degradable skin gels acquired a high growth price likened with cells in peptide-free skin gels (in 15?mL falcon tubes culturing the pellets in 500 then? D control or chondrogenic media as differentiation handles. The mass media was transformed every 3 times. Mechanical properties of hydrogels Degradable and non-degradable hydrogels customized with 100?Meters RGD, 100?Meters GFOGER, or nonmodified were cultured in cell lifestyle moderate in the incubator for 24?l after creation. Compressive moduli of the hydrogels had been tested using a structure analyzer (TA.XTPlus; Steady Microsystems). Examples had been pressurized without preload at a swiftness of 0.01?millimeter/s i9000 and the potent power was measured in 0.1?mm compression, where the gels showed linear behavior. Youngs moduli had been computed regarding to the formulation: where tension is certainly ...
Protein delivery is often used in tissue engineering applications to control differentiation processes, but is limited by protein instability and cost. An alternative approach is to control the cellular microenvironment through biomaterial-mediated sequestration of cell-secreted proteins important to differentiation. Thus, we utilized heparin-based microparticles to modulate cellular differentiation via protein sequestration in an in vitro model system of endochondral ossification. Heparin and poly(ethylene-glycol) (PEG; a low-binding material control)-based microparticles were incorporated into ATDC5 cell spheroids or incubated with ATDC5 cells in transwell culture. Reduced differentiation was observed in the heparin microparticle group as compared to PEG and no microparticle-containing groups. To determine if observed changes were due to sequestration of cell-secreted protein, the proteins sequestered by heparin microparticles were analyzed using SDS-PAGE and mass spectrometry. It was found ...
Clone DB105 recognizes the CD44 antigen. CD44 is a marker for many types of cancer stem cells (CSCs), including breast CSCs that possess higher tumorigenicity and metastatic potential, colorectal, pancreatic, and prostate CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, CD44 plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. - Österreich
However, when cancer cells secrete fresh material into the ECM, it disrupts the chemical and physical composition of the native ECM surrounding healthy cells, degrading the local environment.. Therefore, understanding how the cellular microenvironment is altered due to cancer cells and measuring these changes quantitatively could be vital in understanding the progression of cancer, IISc noted.. In the current study, the researchers discovered that as the nanomotors approached the cancer cell membrane, they stuck to the matrix more strongly than they would to normal cells.. To measure how strongly the nanomotors bound to the matrix, the team calculated the magnetic field strength required to overcome the adhesive force, and move forward, it was stated.. This means that the cancer cells are doing something.. So, we did some measurements and discovered that it [the adhesive force] depended on the type of cells, the strength of interaction and also which side of the cell the nanomotor ...
Organs-on-Chip - Mimicking the in-vivo cellular microenvironment During more than 100 years cell biologists have been culturing cells on flat and hard substrates, called Petri dishes. Plastics replaced glass, but the plates Julius Richard Petri invented in 1887 havent changed and are still widely used today. Although an instrumental tool for life-saving medical breakthroughs, like Sir Alexander Flemings discovery of penicillin, the in-vitro environment provided by Petri dishes widely differs from the native environments the cells are accustomed to in the body. As a result, they often lose their original functions and are no longer suitable to be used as models to test for instance drug candidates. The pharmaceutical industry increasingly recognized this fact and turns more and more to new in-vitro models, such as organs-on-chip, that more accurately represent the milieu in the human body. Organs-on-chip are advanced in-vitro models that mimic the smallest functional unit of an organ. These ...
Scientists describe the optimization of the fabrication process for a novel, detailed, 3D cell culture platform based on direct laser written tubular microtowers and human neuronal cells.
Most cancer deaths are due to spreading of the primary tumor to one or several secondary sites, in a process called metastasis. These metastases are often more difficult to treat than the primary tumor and their presence marks severe progression of the disease. Tumor cell metastasis involves cell migration through heterogenous microenvironments in tissues, along anatomical features such as blood vessels and nerves, and into and out of vasculature. Mounting evidence has accumulated, demonstrating that metastatic tumor cells can migrate via several modes or mechanisms of migration, and can switch between these modes depending on the specific features of the microenvironment. Our research in this area is focused on understanding how distinct physical and biochemical cues from the tumor cells microenvironment affect the phenotype, biological signaling, mechanical properties, and interactions with other cells during metastasis. We are interested in metastasis to the brain, which is especially ...
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
Background:M. tuberculosis infection either induces or inhibits host cell death, depending on the bacterial strain and the cell microenvironment. There is evidence suggesting a role for mitochondria in...
Date: September 24, 2008 at 11:44 am PST. In Reply to: BKT EXPERTS PLEASE HELP! posted by dee on August 10, 2008 at 2:48 pm:. Hey I just read your post. I do both Japanese & Brazilian Straightening. I like both a lot. But there are a few things to take into consideration before making your choise.. 1 - Japanese Straightening is a day trip to the salon. Granted it is fabulous, but I can take upwards of 5 hours depending on the length & texture of your hair.. 2 - With Japanese Straightening you cannot have any other chemical services done. So its either you want it Straight, or you want to have fun with color. 3 - Japanese Starightening can be up to $1500, where as Brazilian straightening is usually about $250. 4 - Brazilian straightening is coating, it does not break and restructure the disulfide bonds in your hair, as Japanese Straightening does.. 5 - With Brazilian you can color your hair, which should be done a week before, as to not strip the straightener out of your hair.. 6 - Thr Brazilian ...
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Enabling investigators to establish stem cell lines in their laboratories is the primary goal of the WiCell Stem Cell Bank. To this aim, WiCell provides protocols for every available stem cell line. The protocols are also shipped with every vial of material. Many cell lines are available in multiple culture platforms (i.e., combinations of medium and matrix). Culture platforms and associated protocols for each cell line are accessible when viewing cell line specific information.
The book describes strategies to exploit chemical cues in cell differentiation processes with emphasis on purification and characterization of exosomes and secreted vesicles, plus micro and non-coding RNAs that have been demonstrated to control the tuning of the in vivo micro and macro environment.
Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of
Engineering cellular microenvironments that more accurately reflect the in vivo situation is now recognized as being crucial for the improvement of the in vitro viability and in vivo-like function of cells or tissues. Microfluidic technologies have been increasingly applied since the late 1990s for this purpose, with a growing number of examples of perfused cell and tissue cultures in microfluidic chambers and channels. The well-defined solution flows provided by microfluidics mean enhanced cell growth and function through improved nutrient delivery and waste removal. Additional benefits include the implementation of well-defined temporal and spatial (bio)chemical gradients, and mechanical signals that cells experience in their natural environment. Because the ability to culture cells and tissue under such controlled conditions leads to cellular function that is distinctly more organ-like, the microfluidic systems used are now referred to as organs-on-a-chip or microphysiological systems. ...
GD-OCM combines high sectioning capability of optical coherence tomography (OCT) and high lateral resolution of confocal microscopy to achieve high-contrast imaging with volumetric cellular resolution of 2 μm across a thickness of up to 2 mm in tissue. The current GD-OCM system fits on a movable cart. The handheld scanning probe is attached to an articulated arm that can be easily adjusted. The light source is a superluminescent diode laser centered at 840 nm with 100 nm FWHM (BroadLighter D-840-HP-I, Superlum®, Ireland). The microscope objective probe incorporates a liquid lens, which allows dynamic-focusing in order to image different depths of the sample. A custom dispersion compensator and a custom spectrometer with a high-speed CMOS line camera (spl4096-70km, Basler Inc.) are used to acquire the spectral information. We recently implemented and will also present a parallelized Multi-Graphic Processing Units (GPU) architecture of the system to allow real-time visualization of the sample. ...
Cecilia Sahlgren is a part-time Associate Professor at the TU/e department of Biomechanical Engineering (research group Soft Tissue Engineering & Mechanobiology) and Professor of Cell Biology at Åbo Akademi University in Turku, Finland. Her research aims at understanding the basic molecular principles of signaling mechanisms regulating cell fate choices of stem cells in cardiovascular tissue engineering. Another important goal is to develop technology to specifically monitor and tune these signals at will in specific cell populations, in order to steer stem cell fate and curtail disease activities. The specific focus of her research is on the role and regulation of the evolutionary conserved Notch signaling pathway, a key regulator of stem cell function. The main objectives are: 1) to understand how the cellular microenvironment influences Notch signaling activities and how this impinges on cell identity and function 2) to understand how Notch activity in stem cells influence remodelling of the ...
Research Summary: Current fibrin matrices/sealants used for wound healing applications utilize high concentrations of fibrinogen and thrombin, forming a dense matrix in order to form a stable clot. We have designed materials to be incorporated into fibrin scaffolds that will create a mechanically robust clot, but allow for increased cell infiltration and regeneration. As a member of the IGERT program and Thomas Barkers Laboratory of Matrix Biology and Engineering, the overall goals of my current research are to engineer fibrin networks to promote endogenous progenitor cell homing and infiltration for enhanced wound healing and tissue regeneration. Through incorporating ultra low cross-linked pNIPAm microgels into fibrin gels, we can modulate the matrix architecture and mechanical properties of the local cellular microenvironment. Additionally, we aim to load microgels with chemokines to recruit specific cell populations to sites of tissue injury. Through harnessing the chemistry and physics of ...
Many forms of solid tumor have a characteristic feature known as hypoxia, which describes a low or non-existent presence of oxygen in the cellular microenvironment. This decrease in oxygen causes activation of the hypoxia inducible factor (HIF) pathway, which activates the transcription of many genes that cause cell proliferation, metastasis, increased glycolysis and angiogenesis. Increased HIF expression has been linked with poor patient prognosis, increased malignancy, and therapeutic resistance. Previous work in our lab has identified 1 and 2 as inhibitors of the HIF pathway, specifically as disrupters of the p300-HIF-1α complex formation. A library of sulfonamide analogs has been designed and synthesized with the intent of examining the SAR of this series of compounds and improving potency and physicochemical properties as compared with lead compounds 1 and 2. At the end, we have achieved a thorough understanding of the structural features critical for future optimization work ...
Conditions leading to unrepaired DNA double-stranded breaks are potent inducers of genetic instability. Systemic conditions may lead to fluctuation of hydrogen ions in the cellular microenvironment and we show that small variations in extracellular pH, termed suboptimal pHe, can decrease the efficiency of DNA repair in the absence of intracellular pH variation. Recovery from bleomycin-induced DNA double-stranded breaks in fibroblasts proceeded less efficiently at suboptimal pHe values ranging from 7.2 to 6.9, as shown by the persistence of repair foci, reduction of H4K16 acetylation and chromosomal instability, while senescence or apoptosis remained undetected. By allowing escape from these protective mechanisms, suboptimal pHe may therefore enhance the genotoxicity of double-stranded breaks, leading to genetic instability. ...
Delta-like 4 (Dll4) is a ligand of the Notch pathway family which has been widely studied in the context of tumor angiogenesis, its blockade shown to result in non-productive angiogenesis and halted tumor growth. As Dll4 inhibitors enter the clinic, there is an emerging need to understand their side effects, namely the systemic consequences of Dll4:Notch blockade in tissues other than tumors. The present study focused on the effects of systemic anti-Dll4 targeting in the bone marrow (BM) microenvironment. Here we show that Dll4 blockade with monoclonal antibodies perturbs the BM vascular niche of sub-lethally irradiated mice, resulting in increased CD31(+), VE-Cadherin(+) and c-kit(+) vessel density, and also increased megakaryocytes, whereas CD105(+), VEGFR3(+), SMA(+) and lectin(+) vessel density remained unaltered. We investigated also the expression of angiocrine genes upon Dll4 treatment in vivo, and demonstrate that IGFbp2, IGFbp3, Angpt2, Dll4, DHH and VEGF-A are upregulated, while FGF1 and CSF2
Bioinspired, Non-Covalent Assembly of Materials: Stem cell activities, including self-renewal and differentiation, are strongly influenced by signals present in their microenvironment. Our group is using novel materials chemistry to control the signals present in the local stem cell microenvironment. Our approaches use non-covalent interactions between biological molecules (e.g. proteins, DNA strands) to assemble signaling complexes on cell culture substrates and within extracellular matrices. These non-covalent assembly approaches are being used to understand/control stem cell differentiation and as a mechanism for targeted drug delivery.. Bio-responsive Materials: Biological macromolecules, including proteins and poly(nucleotides), provide the most basic unit of function in living systems. For example, proteins fold to give structurally well-defined three-dimensional macromolecules and in many cases undergo highly complex changes in response to a broad spectrum of environmental cues, including ...
School of Basic Medical Science, Nanjing Medical University - Cited by 1,347 - Brain Tumor Stem Cells Microenvironment Metabolism
BlackRock Income Trust, Inc.s (BKT) (the Trust) investment objective is to manage a portfolio of high-quality securities to achieve both preservation of capital and high monthly income. The Trust seeks to achieve its investment objective by investing at least 65% of its assets in mortgage-backed securities. The Trust invests at least 80% of its assets in securities that are (i) issued or guaranteed by the US government or one of its agencies or instrumentalities or (ii) rated at the time of investment either AAA by S&P or Aaa by Moodys. The Trust may invest directly in such securities or synthetically through the use of derivatives.No assurance can be given that the Trusts investment objective will be achieved. .carousel-header-text { font: 24px/24px AkkuratProRegular, Arial, sans-serif; text-align: center; margin-top: 20px; float: left; } .carousel-subheader { font: 20px/220x AkkuratProRegular, Arial, sans-serif; text-align: center; } .carousel-text { font: 16px/16px AkkuratProRegular,
Dr. Mullen current research work examines the bone marrow microenvironment of acute lymphoblastic leukemia. The current goal of the work is to identify the molecular mechanisms by which nonmalignant cells in the marrow microenvironment that provide antiapoptotic support for leukemia cells. Ultimately this may identify new drug-able targets that may improve leukemia therapy. ...
While most salon do offer Japanese perm, Daisuke Salon knows the proper techniques needed to make the perfect perm. Our stylists are continuously trained by a Japanese stylist on the latest techniques, styles and fashion.. ...
A new cell culture platform allows researchers to observe never-before-seen behaviors of live cancer cells under the microscope, leading to explanations of long-known cancer characteristics.
chains in the Genus database with same CATH superfamily 2KMC A; 3ONS A; 1WXS A; 4DHZ E; 3APD A; 2Z0D B; 3APC A; 1WM2 A; 4IM2 A; 3SU8 X; 4HVB A; 2REX A; 4GN1 A; 3PZD A; 4ZRK A; 2LXC A; 4BKS D; 4GXB A; 4ZDV A; 2BYF A; 2DAF A; 2KDI A; 2L8J A; 3N32 A; 5IA7 A; 3WAN A; 2V3S A; 3WAM A; 4A3O A; 4EZJ A; 4KSK C; 2KTF A; 1V2Y A; 1V86 A; 4BKT A; 2L0T A; 4HPL B; 1WMH A; 3K9P B; 2GBK A; 4RMA A; 4FJV B; 5FR6 A; 3WAL A; 4AWJ A; 3T8M A; 2KXJ A; 2N9E B; 3I3T B; 3ZNZ B; 4NQK E; 3MJW A; 3RD2 A; 2HTH A; 4G11 A; 3R3M A; 1UZX B; 2KQS A; 3RUI B; 1WE6 A; 4JL9 A; 2KTR A; 2IO2 B; 2LJ5 A; 1WM3 A; 3DVN V; 4WAA A; 5J2R A; 2MLB A; 2D3G A; 2K6Q A; 2DYO A; 3ZUN A; 2JXX A; 4MDK E; 2K8C A; 2KN5 A; 4HXD A; 2KNB A; 4W9L A; 3APF A; 2L7R A; 2N1V A; 1Q0W B; 2ZVO A; 3IVF A; 2BKF A; 3RZF A; 3CSF A; 3L16 A; 3PHX B; 1Q1O A; 3PTF C; 2UWQ A; 3DPD A; 4BFR A; 4DDI D; 3QAQ A; 2JUO A; 2KJ6 A; 2IYD B; 2KC2 A; 1WX8 A; 2L76 A; 3QAR A; 1E8W A; 4MEM A; 5D6J B; 1EF5 A; 3UQA A; 1IP9 A; 2ZEQ A; 4MJS A; 2XEW A; 2J7Q B; 3A9K A; 1JRU A; 2FCN A; 2N4F A; ...
Laboratory of Cellular Biochemistry and Biology. *Namur Research Institute for Life Sciences ... Modérateur-Workshops jeunes chercheurs Télévie : Tumor Microenvironment. Period. 11 Feb 2015. Event type. Other. ...

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