Cellular Microenvironment: Local surroundings with which cells interact by processing various chemical and physical signals, and by contributing their own effects to this environment.Microtechnology: Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Tumor Microenvironment: The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Artificial Intelligence: Theory and development of COMPUTER SYSTEMS which perform tasks that normally require human intelligence. Such tasks may include speech recognition, LEARNING; VISUAL PERCEPTION; MATHEMATICAL COMPUTING; reasoning, PROBLEM SOLVING, DECISION-MAKING, and translation of language.Cell Line, Tumor: A cell line derived from cultured tumor cells.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Water Purification: Any of several processes in which undesirable impurities in water are removed or neutralized; for example, chlorination, filtration, primary treatment, ion exchange, and distillation. It includes treatment of WASTE WATER to provide potable and hygienic water in a controlled or closed environment as well as provision of public drinking water supplies.Waste Disposal, Fluid: The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.Leukemia-Lymphoma, Adult T-Cell: Aggressive T-Cell malignancy with adult onset, caused by HUMAN T-LYMPHOTROPIC VIRUS 1. It is endemic in Japan, the Caribbean basin, Southeastern United States, Hawaii, and parts of Central and South America and sub-Saharan Africa.Cell Aging: The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.Human T-lymphotropic virus 1: A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).Leukemia, T-Cell: A malignant disease of the T-LYMPHOCYTES in the bone marrow, thymus, and/or blood.HTLV-I InfectionsGene Products, tax: Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.Lymphoma, T-Cell: A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.Glioma: Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)Brain Neoplasms: Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Glioblastoma: A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Microwaves: That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.Electromagnetic Radiation: Waves of oscillating electric and MAGNETIC FIELDS which move at right angles to each other and outward from the source.Radio Frequency Identification Device: Machine readable patient or equipment identification device using radio frequency from 125 kHz to 5.8 Ghz.Radio Waves: Electromagnetic waves with frequencies between about 3 kilohertz (very low frequency - VLF) and 300,000 megahertz (extremely high frequency - EHF). They are used in television and radio broadcasting, land and satellite communications systems, radionavigation, radiolocation, and DIATHERMY. The highest frequency radio waves are MICROWAVES.Electromagnetic Fields: Fields representing the joint interplay of electric and magnetic forces.National Academy of Sciences (U.S.): A United States organization of distinguished scientists and engineers established for the purpose of investigating and reporting upon any subject of art or science as requested by any department of government. The National Research Council organized by NAS serves as the principal operating agency to stimulate and support research.Communism: A totalitarian system of government in which a single authoritarian party controls state-owned means of production with the professed aim of establishing a classless society.Neutron Diffraction: The scattering of NEUTRONS by matter, especially crystals, with accompanying variation in intensity due to interference effects. It is useful in CRYSTALLOGRAPHY and POWDER DIFFRACTION.Caves: Geological formations consisting of underground enclosures with access from the surface.Neutrons: Electrically neutral elementary particles found in all atomic nuclei except light hydrogen; the mass is equal to that of the proton and electron combined and they are unstable when isolated from the nucleus, undergoing beta decay. Slow, thermal, epithermal, and fast neutrons refer to the energy levels with which the neutrons are ejected from heavier nuclei during their decay.Scattering, Small Angle: Scattering of a beam of electromagnetic or acoustic RADIATION, or particles, at small angles by particles or cavities whose dimensions are many times as large as the wavelength of the radiation or the de Broglie wavelength of the scattered particles. Also know as low angle scattering. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Small angle scattering (SAS) techniques, small angle neutron (SANS), X-ray (SAXS), and light (SALS, or just LS) scattering, are used to characterize objects on a nanoscale.Scattering, Radiation: The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Cellular Phone: Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.Hospitals, AnimalMotor Vehicles: AUTOMOBILES, trucks, buses, or similar engine-driven conveyances. (From Random House Unabridged Dictionary, 2d ed)Aviation: Design, development, manufacture, and operation of heavier-than-air AIRCRAFT.Facility Design and Construction: Architecture, exterior and interior design, and construction of facilities other than hospitals, e.g., dental schools, medical schools, ambulatory care clinics, and specified units of health care facilities. The concept also includes architecture, design, and construction of specialized contained, controlled, or closed research environments including those of space labs and stations.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Neoplastic Stem Cells: Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.Antigens, CD24: A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)

Screening the cellular microenvironment: a role for microfluidics. (1/313)

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Murine amniotic fluid stem cells contribute mesenchymal but not epithelial components to reconstituted mammary ducts. (2/313)

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Salivary glands of primary Sjogren's syndrome patients express factors vital for plasma cell survival. (3/313)

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Evolving homeostatic tissue using genetic algorithms. (4/313)

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Mathematical descriptions of biochemical networks: stability, stochasticity, evolution. (5/313)

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More than a feeling: discovering, understanding, and influencing mechanosensing pathways. (6/313)

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Toll-like receptors are key players in neurodegeneration. (7/313)

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Nattectin a fish C-type lectin drives Th1 responses in vivo: licenses macrophages to differentiate into cells exhibiting typical DC function. (8/313)

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The colon cancer stem cell microenvironment holds keys to future cancer therapy.s profile, publications, research topics, and co-authors
Abstract While most of the in vitro cultures are carried out on bi-dimensional (2D) substrates, most of the in vivo extracellular matrices are threedimensional (3D). Consequently cells behave differently on 2D substrates as a way to self-adaptation to a non-physiological environment. This fact has encouraged the development of more relevant culture conditions seeking to provide more representative models for biomedicine (e.g. cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. Different 3D culture systems have been established though their variability and complexity hinder their standardisation in common cell culture procedures. So, this thesis deals with the dimensionality issue in cell/material interactions and introduces sandwich-like microenvironments as a versatile tool to study cell behaviour. Cells cultured within this system use both dorsal and ventral receptors to adhere and spread, undergoing important changes with ...
The objective of the presented research is to examine the relationship between the cellular microenvironment and biochemical response of metastatic cells. Clinically recognized as a trait of cancer progression, the cellular microenvironment can have variable and distinct mechanical properties that are processed via cellular mechanosensing, resulting in a cellular biochemical response. A range of studies investigating the interactions between the cellular micromechanical environment and the cells molecular response during disease progression have been made, yet remain absent of quantitative characterization of many of these coordinated responses. The fundamental inquiry that drives the following research attempts to elucidate how a cell perceives the physical microenvironment and converts that signal to a biochemical response. With the goal of providing insight to such responses, the presented research seeks to elucidate the following questions: (1) What are the integrated effects of ECM ...
Several reports have pointed out that the ECM inhibits regeneration and suppresses plasticity in the adult CNS (Berardi et al., 2004; Fitch and Silver, 2008; Gervasi et al., 2008). By comparison, the role of the ECM in the stem and precursor cell microenvironment remains obscure, although the cellular microenvironment is crucial for stem cell maintenance and self-renewal (Scadden, 2006). Focusing on OPCs as a model, we reveal an inhibitory function of the ECM glycoprotein Tnc on differentiation and disentangle molecular details of ECM pathways designed to suppress the differentiation of a committed neural precursor cell. We show that Tnc suppresses the expression of MBP in oligodendrocytes both in vitro and in vivo during postnatal forebrain development, confirming its biological significance. The underlying signaling cascade requires the cell surface receptor Cntn1 and the associated Fyn kinase, and involves the regulation of the signaling adaptor and splice regulator Sam68. We further document ...
Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression ...
J. S. Miller and West, J. L., "Biomimetic hydrogels to support and guide tissue formation", in Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications, A. Khademhosseini, Borenstein, J., Toner, M., and Takayama, S. 2008, pp. 101-120. ...
The magic to killing cancer cells might not be in the bullet, but in the gunpowder that accompanies it, according to researchers at the University of Pittsburgh Cancer Institute (UPCI), partner with UPMC CancerCenter.
Living systems are made of complex architectural organization of various cell types in defined microenvironments. The intricate interactions between different cell types control the specific functions of the associated tissues, such as the functions of native liver and cardiac tissues, metastasis and invasion of tumors, and embryonic development.
Elasticity of cellular microenvironments strongly influences cell motility, phagocytosis, growth and differentiation. Currently, the relationship between the cell behaviour and matrix stiffness is being studied for cells seeded on planar substrates, however in three-dimensional (3D) microenvironments cells may experience mechanical signalling that is distinct from that on a two-dimensional matrix. We report a microfluidic approach for high-throughput generation of 3D microenvironments with different elasticity for studies of cell fate. The generation of agarose microgels with different elastic moduli was achieved by (i) introducing into a microfluidic droplet generator two streams of agarose solutions, one with a high concentration of agarose and the other one with a low concentration of agarose, at varying relative volumetric flow rate ratios of the two streams, and (ii) on-chip gelation of the precursor droplets. At 37 °C, the method enabled a ~35-fold variation of the shear elastic modulus ...
Injury-induced stem-cell niche are defined as cellular microenvironments that are generated during tissue injury, these environments are triggered by injury and the local responses of support cells, and enable the possibility of repair by endogenous or transplanted neural stem cells. These environments have been demonstrated in several injury models, most notable in the CNS. The term was coined by Jaime Imitola and Evan Y. Snyder when they demonstrated that astrocytes and endothelial cells during stroke are able to create a permissive environment for neural regeneration,[1] that is most striking for exogenous transplanted neural stem cells. Previous work by the Snyder Laboratory have shown that the interactions between NSCs and local cells is reciprocal, underlying a bystander beneficial effect of neural stem cells without neural differentiation, once thought to be the only mechanism for therapeutical benefit of stem cells in CNS injury.[2]. More recently these findings have been reproduced and ...
It isnt about who discovers the remedy first. Its about getting a cure - fast... BCBC awards $5 million for cell therapy analysis in type 1 diabetes Maike Sander, MD, associate professor of pediatrics and cellular & molecular medicine at the University of California, NORTH PARK School of Medication has been awarded nearly $5 million by the Beta Cell Biology Consortium to business lead an interdisciplinary group in cell therapy analysis for type 1 diabetes. Sander will business lead a united group of domestic and worldwide collaborators, with the purpose of generating substitute insulin-making beta cells from patient-derived induced pluripotent stem cells. The $4,950,000 grant is distributed to Karl Willert, PhD, director of the UCSD Human being Stem Cell Core Service, wholl apply a screening system for cellular microenvironments to beta cell differentiation Additional individuals consist of labs at the University of Pennsylvania and in Barcelona, Spain, in addition to a San Diego-structured ...
2509 Nitric Oxide (NO•), an important signaling molecule and a component of inflammatory response, is also involved in tumorigenesis. However, the quantity of NO• and the nature of the cellular microenvironment influences the role of NO• in tumor development. We used a genetic strategy to test the hypothesis that an inflammatory microenvironment with an enhanced level of NO• accelerates spontaneous tumor development. C. parvum-induced inflammation and increased nitric oxide synthase-2 (NOS2) expression which coincided with accelerated spontaneous tumor development, mostly lymphomas, in p53-/-NOS2+/+ C57BL6 mice as compared to the controls (p=0.001). However, p53-/-NOS2-/- mice did not show any difference in tumor latency between C. parvum-treated and control groups. C. parvum-treated p53-/-NOS2+/+ mice showed that tumor development was preceded by a higher expression of NOS2 and phosphorylated Akt-Ser 473 (pAkt-Ser473) in spleen, increased cell proliferation measured by Ki-67 ...
Mammalian cell culture has long been an invaluable tool in cell biology, drug discovery, and regenerative medicine. When cell culture techniques were first developed, 3-Dimensional (3D) systems were utilized. That rapidly changed due to cost and efficiency concerns resulting in cell culture being performed today with adherent cells grown on flat and rigid two-dimensional (2D) substrates, including polystyrene or glass. Advances in our understanding of cell physiology and failures in clinical trials have provided the impetus to move away from 2D systems and back to a more in-vivo-like 3D environment. The advance of these new technologies and screening methodologies have allowed scientists to assess more realistic functional capabilities of cells. The following course will focus on the cellular microenvironment and its importance when developing and screening cell-based assays using primary, stem cell, and immortalized cultures in 3D systems.. ...
Protein delivery is often used in tissue engineering applications to control differentiation processes, but is limited by protein instability and cost. An alternative approach is to control the cellular microenvironment through biomaterial-mediated sequestration of cell-secreted proteins important to differentiation. Thus, we utilized heparin-based microparticles to modulate cellular differentiation via protein sequestration in an in vitro model system of endochondral ossification. Heparin and poly(ethylene-glycol) (PEG; a low-binding material control)-based microparticles were incorporated into ATDC5 cell spheroids or incubated with ATDC5 cells in transwell culture. Reduced differentiation was observed in the heparin microparticle group as compared to PEG and no microparticle-containing groups. To determine if observed changes were due to sequestration of cell-secreted protein, the proteins sequestered by heparin microparticles were analyzed using SDS-PAGE and mass spectrometry. It was found ...
Clone DB105 recognizes the CD44 antigen. CD44 is a marker for many types of cancer stem cells (CSCs), including breast CSCs that possess higher tumorigenicity and metastatic potential, colorectal, pancreatic, and prostate CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, CD44 plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. - Österreich
Organs-on-Chip - Mimicking the in-vivo cellular microenvironment During more than 100 years cell biologists have been culturing cells on flat and hard substrates, called Petri dishes. Plastics replaced glass, but the plates Julius Richard Petri invented in 1887 havent changed and are still widely used today. Although an instrumental tool for life-saving medical breakthroughs, like Sir Alexander Flemings discovery of penicillin, the in-vitro environment provided by Petri dishes widely differs from the native environments the cells are accustomed to in the body. As a result, they often lose their original functions and are no longer suitable to be used as models to test for instance drug candidates. The pharmaceutical industry increasingly recognized this fact and turns more and more to new in-vitro models, such as organs-on-chip, that more accurately represent the milieu in the human body. Organs-on-chip are advanced in-vitro models that mimic the smallest functional unit of an organ. These ...
Scientists describe the optimization of the fabrication process for a novel, detailed, 3D cell culture platform based on direct laser written tubular microtowers and human neuronal cells.
Most cancer deaths are due to spreading of the primary tumor to one or several secondary sites, in a process called metastasis. These metastases are often more difficult to treat than the primary tumor and their presence marks severe progression of the disease. Tumor cell metastasis involves cell migration through heterogenous microenvironments in tissues, along anatomical features such as blood vessels and nerves, and into and out of vasculature. Mounting evidence has accumulated, demonstrating that metastatic tumor cells can migrate via several modes or mechanisms of migration, and can switch between these modes depending on the specific features of the microenvironment. Our research in this area is focused on understanding how distinct physical and biochemical cues from the tumor cells microenvironment affect the phenotype, biological signaling, mechanical properties, and interactions with other cells during metastasis. We are interested in metastasis to the brain, which is especially ...
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
CYTOO is a Contract Research Organization developing more physiological cell-based assays fully compatible with High-Content Screening and High-Content Analysis. CYTOO has pioneered the ability to control cells microenvironment and has developed a platform of micropattern technologies to enable significantly greater physiologically relevant cellular models in comparison to
Background:M. tuberculosis infection either induces or inhibits host cell death, depending on the bacterial strain and the cell microenvironment. There is evidence suggesting a role for mitochondria in...
Date: September 24, 2008 at 11:44 am PST. In Reply to: BKT EXPERTS PLEASE HELP! posted by dee on August 10, 2008 at 2:48 pm:. Hey I just read your post. I do both Japanese & Brazilian Straightening. I like both a lot. But there are a few things to take into consideration before making your choise.. 1 - Japanese Straightening is a day trip to the salon. Granted it is fabulous, but I can take upwards of 5 hours depending on the length & texture of your hair.. 2 - With Japanese Straightening you cannot have any other chemical services done. So its either you want it Straight, or you want to have fun with color. 3 - Japanese Starightening can be up to $1500, where as Brazilian straightening is usually about $250. 4 - Brazilian straightening is coating, it does not break and restructure the disulfide bonds in your hair, as Japanese Straightening does.. 5 - With Brazilian you can color your hair, which should be done a week before, as to not strip the straightener out of your hair.. 6 - Thr Brazilian ...
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Enabling investigators to establish stem cell lines in their laboratories is the primary goal of the WiCell Stem Cell Bank. To this aim, WiCell provides protocols for every available stem cell line. The protocols are also shipped with every vial of material. Many cell lines are available in multiple culture platforms (i.e., combinations of medium and matrix). Culture platforms and associated protocols for each cell line are accessible when viewing cell line specific information.
Engineering cellular microenvironments that more accurately reflect the in vivo situation is now recognized as being crucial for the improvement of the in vitro viability and in vivo-like function of cells or tissues. Microfluidic technologies have been increasingly applied since the late 1990s for this purpose, with a growing number of examples of perfused cell and tissue cultures in microfluidic chambers and channels. The well-defined solution flows provided by microfluidics mean enhanced cell growth and function through improved nutrient delivery and waste removal. Additional benefits include the implementation of well-defined temporal and spatial (bio)chemical gradients, and mechanical signals that cells experience in their natural environment. Because the ability to culture cells and tissue under such controlled conditions leads to cellular function that is distinctly more organ-like, the microfluidic systems used are now referred to as "organs-on-a-chip" or "microphysiological systems". ...
GD-OCM combines high sectioning capability of optical coherence tomography (OCT) and high lateral resolution of confocal microscopy to achieve high-contrast imaging with volumetric cellular resolution of 2 μm across a thickness of up to 2 mm in tissue. The current GD-OCM system fits on a movable cart. The handheld scanning probe is attached to an articulated arm that can be easily adjusted. The light source is a superluminescent diode laser centered at 840 nm with 100 nm FWHM (BroadLighter D-840-HP-I, Superlum®, Ireland). The microscope objective probe incorporates a liquid lens, which allows dynamic-focusing in order to image different depths of the sample. A custom dispersion compensator and a custom spectrometer with a high-speed CMOS line camera (spl4096-70km, Basler Inc.) are used to acquire the spectral information. We recently implemented and will also present a parallelized Multi-Graphic Processing Units (GPU) architecture of the system to allow real-time visualization of the sample. ...
Cecilia Sahlgren is a part-time Associate Professor at the TU/e department of Biomechanical Engineering (research group Soft Tissue Engineering & Mechanobiology) and Professor of Cell Biology at Åbo Akademi University in Turku, Finland. Her research aims at understanding the basic molecular principles of signaling mechanisms regulating cell fate choices of stem cells in cardiovascular tissue engineering. Another important goal is to develop technology to specifically monitor and tune these signals at will in specific cell populations, in order to steer stem cell fate and curtail disease activities. The specific focus of her research is on the role and regulation of the evolutionary conserved Notch signaling pathway, a key regulator of stem cell function. The main objectives are: 1) to understand how the cellular microenvironment influences Notch signaling activities and how this impinges on cell identity and function 2) to understand how Notch activity in stem cells influence remodelling of the ...
Research Summary: Current fibrin matrices/sealants used for wound healing applications utilize high concentrations of fibrinogen and thrombin, forming a dense matrix in order to form a stable clot. We have designed materials to be incorporated into fibrin scaffolds that will create a mechanically robust clot, but allow for increased cell infiltration and regeneration. As a member of the IGERT program and Thomas Barkers Laboratory of Matrix Biology and Engineering, the overall goals of my current research are to engineer fibrin networks to promote endogenous progenitor cell homing and infiltration for enhanced wound healing and tissue regeneration. Through incorporating ultra low cross-linked pNIPAm microgels into fibrin gels, we can modulate the matrix architecture and mechanical properties of the local cellular microenvironment. Additionally, we aim to load microgels with chemokines to recruit specific cell populations to sites of tissue injury. Through harnessing the chemistry and physics of ...
Many forms of solid tumor have a characteristic feature known as hypoxia, which describes a low or non-existent presence of oxygen in the cellular microenvironment. This decrease in oxygen causes activation of the hypoxia inducible factor (HIF) pathway, which activates the transcription of many genes that cause cell proliferation, metastasis, increased glycolysis and angiogenesis. Increased HIF expression has been linked with poor patient prognosis, increased malignancy, and therapeutic resistance. Previous work in our lab has identified 1 and 2 as inhibitors of the HIF pathway, specifically as disrupters of the p300-HIF-1α complex formation. A library of sulfonamide analogs has been designed and synthesized with the intent of examining the SAR of this series of compounds and improving potency and physicochemical properties as compared with lead compounds 1 and 2. At the end, we have achieved a thorough understanding of the structural features critical for future optimization work ...
Delta-like 4 (Dll4) is a ligand of the Notch pathway family which has been widely studied in the context of tumor angiogenesis, its blockade shown to result in non-productive angiogenesis and halted tumor growth. As Dll4 inhibitors enter the clinic, there is an emerging need to understand their side effects, namely the systemic consequences of Dll4:Notch blockade in tissues other than tumors. The present study focused on the effects of systemic anti-Dll4 targeting in the bone marrow (BM) microenvironment. Here we show that Dll4 blockade with monoclonal antibodies perturbs the BM vascular niche of sub-lethally irradiated mice, resulting in increased CD31(+), VE-Cadherin(+) and c-kit(+) vessel density, and also increased megakaryocytes, whereas CD105(+), VEGFR3(+), SMA(+) and lectin(+) vessel density remained unaltered. We investigated also the expression of angiocrine genes upon Dll4 treatment in vivo, and demonstrate that IGFbp2, IGFbp3, Angpt2, Dll4, DHH and VEGF-A are upregulated, while FGF1 and CSF2
BlackRock Income Trust, Inc.s (BKT) (the Trust) investment objective is to manage a portfolio of high-quality securities to achieve both preservation of capital and high monthly income. The Trust seeks to achieve its investment objective by investing at least 65% of its assets in mortgage-backed securities. The Trust invests at least 80% of its assets in securities that are (i) issued or guaranteed by the US government or one of its agencies or instrumentalities or (ii) rated at the time of investment either AAA by S&P or Aaa by Moodys. The Trust may invest directly in such securities or synthetically through the use of derivatives.No assurance can be given that the Trusts investment objective will be achieved. .carousel-header-text { font: 24px/24px AkkuratProRegular, Arial, sans-serif; text-align: center; margin-top: 20px; float: left; } .carousel-subheader { font: 20px/220x AkkuratProRegular, Arial, sans-serif; text-align: center; } .carousel-text { font: 16px/16px AkkuratProRegular,
Dr. Mullen current research work examines the bone marrow microenvironment of acute lymphoblastic leukemia. The current goal of the work is to identify the molecular mechanisms by which nonmalignant cells in the marrow microenvironment that provide antiapoptotic support for leukemia cells. Ultimately this may identify new "drug-able" targets that may improve leukemia therapy. ...
While most salon do offer Japanese perm, Daisuke Salon knows the proper techniques needed to make the perfect perm. Our stylists are continuously trained by a Japanese stylist on the latest techniques, styles and fashion.. ...
A new cell culture platform allows researchers to observe never-before-seen behaviors of live cancer cells under the microscope, leading to explanations of long-known cancer characteristics.
TY - JOUR. T1 - Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. AU - Nguyen, Aaron N.. AU - Stebbins, Elizabeth G.. AU - Henson, Margaret. AU - OYoung, Gilbert. AU - Choi, Sun J.. AU - Quon, Diana. AU - Damm, Debby. AU - Reddy, Mamatha. AU - Ma, Jing Y.. AU - Haghnazari, Edwin. AU - Kapoun, Ann M.. AU - Medicherla, Satyanarayana. AU - Protter, Andy. AU - Schreiner, George F.. AU - Kurihara, Noriyoshi. AU - Anderson, Judy. AU - Roodman, G. David. AU - Navas, Tony A.. AU - Higgins, Linda S.. PY - 2006/6/10. Y1 - 2006/6/10. N2 - The multiple myeloma (MM) bone marrow (BM) microenvironment plays a critical role in supporting tumor growth and survival as well as in promoting formation of osteolytic lesions. Recent results suggest that the p38 mitogen-activated protein kinase (MAPK) is an important factor in maintaining this activated environment. In this report, we demonstrate that the p38α ...
MicroRNAs: Novel Crossroads between Myeloma Cells and the Bone Marrow Microenvironment. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Edwin L. Steele Laboratory for Tumor Biology. Research Summary. Bench to Bedside and Back. As part of the Edwin L. Steele Laboratory for Tumor Biology, research at the Jain Laboratory focuses on normalizing tumor vessels and microenvironment. A solid tumor is an organ composed of cancer cells and host stromal cells, which are nourished by the vasculature and embedded in an extracellular matrix. The interaction among these cells, the surrounding matrix, and the local cellular microenvironment influences the expression of certain genes, whose products control the pathophysiological characteristics of the tumor, govern tumor progression, and affect the tumors response to various therapies. Blood and lymphatic vessels also serve as conduits for metastatic spread. The over arching goal of our research is to dissect the pathophysiology of the vascular and extra-vascular components of tumors, to determine the role of tumor-host interactions in tumor biology, and ultimately to translate this knowledge ...
ATF6, a membrane-anchored transcription factor from the endoplasmic reticulum (ER) that modulates the cellular response to stress as an effector of the unfolded-protein response (UPR), is a key player in the development of tumors of different origin. reticulum (ER) can be particularly affected by the presence of mutations in secretory proteins or by dynamic changes in the cellular microenvironment, events which are often encountered in cancers. In the ER, these events are sensed by specific sensors, which in turn trigger select Rabbit Polyclonal to CPB2 signaling pathways, collectively named the unfolded-protein response (UPR) (1). The UPR is an adaptive response that allows the cells to either overcome the stress or promote cell death in the case of overwhelming burden (1). Three ER-resident proteins, namely, the protein kinase PKR-like ER kinase (PERK), the inositol-requiring protein 1 alpha (IRE1), and the activating transcription factor 6 alpha (ATF6), have been identified as the major ...
The VHL−/− human RCC cell line 786-O forms tumors in the nude mouse xenograft assay. We showed previously that reintroduction of pVHL into these cells markedly inhibited the ability of these cells to grow as tumors in nude mice (8, 9) without altering their growth characteristics in vitro. These observations led to the hypothesis that the cellular microenvironment participates in pVHL-mediated tumor suppression possibly through regulation of hypoxia-inducible genes and angiogenesis (11). RCC cells lacking wild-type pVHL inappropriately overproduce hypoxia-inducible mRNA under both hypoxic (1% ambient O2) and normoxic (21% ambient O2) conditions (8, 9). Reintroduction of pVHL into these cells restores the normal profile of hypoxia-inducible mRNA regulation and leads to tumor suppression.. The overexpression of hypoxia-inducible genes in VHL−/− cells has been attributed to transcriptional and posttranscriptional events (8, 9), implying that the effects of pVHL could be attributed to ...
Inflammation in obese adipose tissue can be modulated by alterations of the composition and/or interactions of local immune cells. Our finding that CD40KO mice in the DIO model exhibited exacerbated local inflammation in EAT, insulin resistance, and metabolic abnormalities was initially surprising, as CD40 itself is a costimulatory receptor and its deficiency impairs general immune responses. Of particular interest, we found that CD40-deficient CD8+ T cells in EAT produced more proinflammatory cytokines and CCL5 and CCL3, and this was critical for immune cell infiltration and insulin resistance induction in DIO CD40KO mice. We propose that CD40 may play a special role in the microenvironment of obese EAT through regulating CD8+ T-cell function.. In contrast to our observations with CD40KO mice, a previous study showed that CD154/CD40L is a requirement to maintain adipose tissue inflammation in mice (24) and CD154 deficiency attenuates diet-induced steatosis and improves insulin resistance (28). ...
Changing the course of how diseases are treated and ultimately cured requires a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment. To meet this great challenge, we developed the Hyperion™ Imaging System. The Hyperion Imaging System brings proven CyTOF® technology together with imaging capability to empower simultanous interrogation of 4 to 37 protein markers using Imaging Mass Cytometry™ (IMC™). Using this system, you can deeply interrogate tissues and tumors at subcellular resolution while preserving the information in tissue architecture and cellular morphology to uncover new biomarker correlations and cell interactions. With the ability to utilize up to 135 channels to detect additional parameters, the Hyperion Imaging System is ideal to meet your image analysis needs today and well into the future. ...
Define microenvironment: a small or relatively small usually distinctly specialized and effectively isolated habitat (such as a forest canopy) or…
A sample processing system that may be configured to achieve rapid sample processing such as rapid histochemical processing may involve a wave element that can use angular microscopic slide movements to cause repeated elimination and reapplication of a fluidic substance perhaps through the action of capillary motion in order to refresh a microenvironment adjacent to a sample such as a biopsy or other such sample. Through such refreshing of a microenvironment, depletion of the microenvironment is avoided and the time necessary for slide processing may be dramatically shortened.
TY - JOUR. T1 - Role of the bone marrow microenvironment in multiple myeloma. AU - Roodman, G. David. PY - 2002/11/1. Y1 - 2002/11/1. N2 - On June 26-27, 2001, the Sixth Research Roundtable in Multiple Myeloma, entitled "The Role of the Bone Microenvironment in Multiple Myeloma," was held and focused on the biology of cell-to-cell interactions, the mediators of bone disease, and novel treatment strategies for myeloma. Studies on cell-cell interactions showed that vascular cell adhesion molecule 1, expressed by local endothelial and stromal cells, binds to tumor cell surface integrins in which expression may be increased by tumor cell-derived chemokines such as macrophage inflammatory protein (MIP) 1α. These adhesive interactions increase production and release of vascular endothelial growth factor (VEGF). Studies on myeloma bone disease showed the ligand for receptor activator of nuclear transcription factor-κB (RANKL) was expressed on tumor cells and stromal cells associated with myeloma ...
Extracellular matrix (ECM)1 serves as the immediate microenvironment for interactions with the cell surface, besides providing the structural support for all tissues. The ECM is not static. Rather, it is dynamic in nature with a continuous turnover of its protein constituents and growth factor pools. A major determinant of ECM turnover and integrity is the extracellular proteolytic balance between secreted matrix metalloproteinases (MMPs) and their biological inhibitors (TIMPs) (for reviews see Matrisian 1992; Denhardt et al. 1993; Mignatti and Rifkin 1993). The function of extracellular proteolysis extends beyond ECM degradation to the processing of cell surface receptors and ligands and release of protein-bound growth factors (for review see Werb 1997). Therefore, it is conceivable that extracellular proteolytic activity within the cellular microenvironment can directly impact cell proliferation. Despite transgenic studies showing that cellular proliferation is altered by ectopic expression of ...
Traumatic and nontraumatic brain injury results from severe disruptions in the cellular microenvironment leading to massive loss of neuronal populations and increased neuroinflammation. The progressive cascade of secondary events, including ischemia, inflammation, excitotoxicity, and free-radical release, contribute to neural tissue damage. NLRX1 is a member of the NLR family of pattern recognition receptors and is a potent negative regulator of several pathways that significantly modulate many of these events. Thus, we hypothesized that NLRX1 limits immune system signaling in the brain following trauma. To evaluate this hypothesis, we used Nlrx1−/− mice in a controlled cortical impact (CCI) injury murine model of traumatic brain injury (TBI). In this article, we show that Nlrx1−/− mice exhibited significantly larger brain lesions and increased motor deficits following CCI injury. Mechanistically, our data indicate that the NF-κB signaling cascade is significantly upregulated in ...
The conditions of the cellular microenvironment in complex multicellular organisms fluctuate, enforcing permanent adaptation of cells at multiple regulatory levels. Covalent post-translational modifications of proteins provide the short-term response tools for cellular adjustment and growing evidence supports the possibility that protein tyrosine nitration is part of this cellular toolkit and not just a marker for oxidative damage. We have demonstrated that protein tyrosine nitration fulfils the major criteria for signalling and suggest that the normally highly regulated process may lead to disease upon excessive or inappropriate nitration.. ...
Baccin, C.*, Al-Sabah*, J., Velten, L.*@, Helbling, P. M., Grunschlager, F., Hernandez-Malmierca, P., Nombela-Arrieta, C., Steinmetz, L. [email protected], Trumpp, [email protected], & Haas, [email protected] (2019). Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization. Nature Cell Biology. doi: 10.1038/s41556-019-0439-6 *Shared first authors, @ Joint Supervising authors. ...
wrote: > MSE Undergraduate Research Program Invited Talk > > Friday, August 26, 2011 > Paul G. Allen Auditorium (CIS-X 101) > Lecture at 3:30PM > > Bioinspired Soft Materials to Direct Stem Cell Function and Tissue > Regeneration > > *Kevin E. Healy* > > Jan Fandrianto Distinguished Professor in Engineering > > Departments of Materials Science and Engineering, and Bioengineering > > University of California at Berkeley > > > > Highly regulated signals in the stem cell microenvironment, such as growth > factor presentation and concentration, matrix stiffness, and ligand adhesion > density have been implicated in modulating stem cell proliferation and > maturation. Therefore, it is desirable to have independent control over both > the biochemical and mechanical cues presented to cells to analyze their > relative and combined effects on stem cell function. Accordingly, we have > developed synthetic hydrogels and biointerfaces to assess the effects of > adhesion ligand presentation and material ...
Our research goal is to understand how specific tissues, such as bone, control the fate of cancer cells - regulating their survival, dormancy and subsequent activation into metastatic tumours. We aim to generate new therapies for early intervention in metastatic disease and prevention of disease recurrence.
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Interactions Between Stem and Progenitor Cells and the Microenvironment (R21) PAS-06-208. NINDS
New findings presented today by CytoReason reveals possible new cellular players in the tumor microenvironment that could impact the treatment process
TY - JOUR. T1 - Control of stem cell fate and function by polymer nanofibers. AU - Rana, Deepti. AU - Leena, Maria. AU - Nithyananth, Manasseh. AU - Pasricha, Renu. AU - Manivasagam, Geetha. AU - Ramalingam, Murugan. PY - 2016/9. Y1 - 2016/9. N2 - Stem cells are considered as an integral part of tissue engineering and regenerative medicine. Cellular functions of stem cells, which are responsible for tissue organization, can be controlled and regulated by providing an appropriate microenvironment, which mimics native stem cell niche. Nanotechnology is a powerful tool for engineering cellular microenvironment in the form of scaffolds. The scaffolds that have nanoscale features, for example, nanofiber, are considered as an effective substratum for tissue regenerative applications because they structurally mimic the native extracellular matrix (ECM). Electrospinning is a technique which produces polymer nanofiber scaffolds with controlled size and orientation of the fibrous structure. These polymer ...
Nicolas Szitas research interests focus on the translation of bioprocessing concepts into microfluidic systems (or Lab-on-a-chip systems). He has particular expertise in the use of advanced microfabrication techniques for polymers (rapid prototyping), glass and silicon. He co-ordinates the research activities of the Microfluidics Laboratory in Biochemical Engineering, and collaborates with the London Centre for Nanotechnology (LCN). Microfluidic devices provide an environment conducive to cell growth, operate with much smaller volumes, reducing the amount of cells and media required, and can be fitted with instrumentation for the real-time monitoring of assays. Additionally, they offer advantages unavailable at the larger scales, such as precise temporal and spatial control over the cellular microenvironment, and the scale-out to platforms of multiple microfluidic systems for high-throughput applications. Microfluidic systems are thus particularly suitable for the development of novel ...
Collagen is a natural and abundant polymer that serves multiple functions in both invertebrates and vertebrates. As collagen is the natural scaffolding for cells, collagen-based hydrogels are regarded as ideal materials for tissue engineering applications since they can mimic the natural cellular microenvironment. Chondrosia reniformis is a marine demosponge particularly rich in collagen, characterized by the presence of labile interfibrillar crosslinks similarly to those described in the mutable collagenous tissues (MCTs) of echinoderms. As a result single fibrils can be isolated using calcium-chelating and disulphide-reducing chemicals. In the present work we firstly describe a new extraction method that directly produces a highly hydrated hydrogel with interesting self-healing properties. The materials obtained were then biochemically and rheologically characterized. Our investigation has shown that the developed extraction procedure is able to extract collagen as well as other proteins and
Using a mouse model developed to study the basal-like subtype, the research team discovered that obesity radically alters the cellular microenvironment of mammary glands in ways favorable to the growth of basal-like tumors. One major change is that obesity promotes a growth factor signaling pathway between the hepatocyte growth factor (HGF) protein and an oncogene known as c-Met that is linked with basal-like cancer formation. In animals with elevated levels of HGF, the development of basal-like tumors increased.. "Our study was fairly unique in that we focused on the role that the surrounding tissue in the breast, known as the stroma, plays in breast cancer onset. Many scientists study the tumor alone, but the stroma soil where the cancer seed grows is important in helping that tumor grow," said Makowski.. Since HGF levels are increased with obesity, the study indicates that public health efforts to prevent obesity in at-risk populations may be a clinically useful way of preventing the ...
Lung cancer cells express different chemokines and chemokine receptors that modulate leukocyte infiltration within tumor microenvironment. The released CXCL1 was functionally linked to recruiting monocytes into lung cancer cell microenvironment. and in Lewis lung carcinomas (LLC) [10]. In human airway epithelium and bronchoalveolar macrophages, monocyte chemoattractant protein-1 (MCP-1) and CXCL1 were buy BAM 7 constitutively expressed and upregulated buy BAM 7 by TNF- but not by lipopolysaccharide (LPS) [11]. In pathological conditions, various cancer and/or cancer cells express different chemokines and chemokine receptor that modulate leukocyte infiltration within tumor microenvironment, tumor growth and metastasis. For example, CXCL1 has been reported to be expressed in melanoma, breast, colon and ovarian cancer [3]. Non-small cell TSPAN11 lung cancer (NSCLC) biopsy specimens have high buy BAM 7 intratumoral concentrations of CXCR2 ligands (CXCL1, CXCL5, and CXCL8) and type 2 cytokines ...
If you have a question about this talk, please contact Ilana Spilka.. Increasing evidence suggests that the tumor heterogeneity is rooted in the existence of a very small sub-population of stem-like cells (CSCs) within the population of tumor cells. Since cancer takes many years to grow, it is important to develop in vitro models to study tumor initiation and progression. In an effort to regulate the cancer cell microenvironment, my laboratory has merged material synthesis, microscale technologies, and cancer biology to develop an engineered 3D matrix to delineate the role of individual factors in the tumor microenvironment on CSC maintenance, self-renewal, and differentiation. I will demonstrate in my talk that engineered matrices can enrich the CSC subpopulation of cancer cells which is potentially useful in cancer drug screening and personalized cancer treatment.. This talk is part of the Three-dimensional cell culture: Innovations in tissue scaffolds and biomimetic systems series.. ...
In response to antigen presentation, helper T lymphocytes (TH cells) initiate store-operated Ca2+ entry (SOCE) and differentiate into effector subtypes such as TH1 and TH2 cells. These cells play essential roles in adaptive immunity and the pathogenesis of various autoimmune and allergic diseases. The differentiation and activity of TH cells are also critically regulated by paracrine and autocrine soluble factors in the cell microenvironment. Previous studies have reported that TH cells produce gamma-aminobutyric acid (GABA) via glutamic acid decarboxylase (GAD) and express A-type GABA receptors (GABAARs), forming an autocrine GABA signaling system. In addition, GABA executes anti-inflammatory actions in TH1-autoimmune diseases. This project sought to examine whether autocrine GABA signaling distinctively regulates the function of different TH effector cells using two unique lines of human TH cells: Jurkat and CCRF-CEM. Our results showed that Jurkat and CCRF-CEM cells exhibited features of TH1 and TH2
Aim 1 will elucidate the impact of variable RNA processing on bovine macrophage response to IFNG stimulation or infection with Toxoplasma or Neospora. It will also include maturation of the bioinformatics pipeline to analyze resulting sequence data.. Aim 2 will decipher the parasites response to changes in the host cell microenvironment. It will exploit dual RNA- and Ribo-seq to reveal Toxoplasma and Neospora transcriptional and translational reprogramming in naïve and IFNG-stimulated macrophages.. The proposed work will yield a comprehensive view of the role for RNA processing in the host and pathogen, as well as new information on the extent of translation of individual gene products. In addition, it will reveal the functions of the numerous recently identified short open-reading frames (sORFs) in cattle, Toxoplasma and Neospora.. Through this project, the student should acquire a solid understanding of molecular biology, immunology, and computational biology. He/she will become proficient ...
Overcoming drug resistance in the bone marrow microenvironment is an unresolved challenge in therapy of multiple myeloma. Despite numerous therapeutic approaches with new agents targeting the bone marrow microenvironment multiple myeloma still remains an incurable condition. Multiple myeloma cells exhibit numerous mechanisms of drug resistance. Constitutive activation of the Phosphatidylinositol-3-kinase (PI3K) is associated with tumour progression in multiple myeloma. Targeting the PI3K/Akt pathway can induce apoptosis in multiple myeloma cells. Therefore the use of PI3K inhibitors is predicted to increase the susceptibility of myeloma cells to anticancer therapy. We investigated the novel selective PI3K inhibitor BAY 80-6946 in four human myeloma cell lines. BAY 80-6946 induced significant inhibition of Akt phosphorylation in a dose-dependent manner being accompanied with an increase of apoptosis and inhibition of cell cycle progression. Moreover BAY 80-6946 could overcome the stimulation ...
Researchers from the University of Illinois have developed a new synthetic tissue microenvironment that may give cancer researchers the next-best look at tumor growth and behavior. The 3D patterned vasculature tumor microenvironment is created by changing the flow rates of fluid channels and the structures are made of alginate that was mixed with breast cancer cells in the outer layer and macrophages on the inner layer. The 3D vascularization, a network of capillaries that carry drugs and other materials are created can go from straight, to snakelike, to any shape, that offers modelling for metastasis, because the vessel architecture can be tuned on the fly ...
Orthogonal cancer targeting strategy using nanomicelles. a Schematic of the process of photoactivation of Titanocene in disseminated cancer cells in the bone marrow microenvironment. The various phases are numbered: 1. Administration of targeted NM-TC; 2. The targeted NM enter the bone marrow from the vasculature and bind to α4β1 receptor on the cancer cells and subsequently deliver the drug to the cell; 3. Administration of radiopharmaceuticals (18FDG), which is typically 1.5-2 h after phase (1); 4. 18FDG enters the cancer cells through the overexpressed Glut transporters on cancer cells; 5. Once the drug and radiopharmaceutical are co-localized in the cancer cells, the former is photoactivated by the latter through CR leading to cell death (6). Notice that since the other vital cells in the bone marrow, such as stem cells and stromal cells, do not express the combination of α4β1 and glut receptors essential for the treatment to work, they would largely remain unaffected causing minimal ...
From the information i have gathered and observed on this popular hair treatment is that, it takes an average of 2-3 hours (plus minus) to complete the whole process from applying the formula to the ultrasonic infrared treatment that helps with the penetration (takes up to 50 mins), and trust me that you will eventually fall sleep because its that relaxing just lying there doing nothing lol. But the end result of it is amazing, it tames your hair the natural way, providing up to 2 months of mess free hair (for me at least) but results do varies from customer to customer as everyone has different types of hair texture ...
Mawi DNA Technologies has launched the iSWAB-Microbiome, a biosampling tool designed to collect and stabilize microbial content and diversity within gut, rectal, vaginal, skin, oral, or soil samples for processing. The tool was developed to produce an accurate representation of the microbiota composition without loss of any taxa from the point of collection to processing, to provide researchers with the ability to analyze microbiome with minimal host gDNA contamination, and to enable analysis of the microbiome DNA and RNA from the same sample, the company said.
Neoplastic disease is inextricably associated with aging. Five out of six cancer-related deaths occur in patients aged 60 years and older. However, the intimate nature of this association is yet to be fully clarified. An important concept emerging from the literature is that aging and cancer do not merely represent two chronologically parallel processes, but they share relevant pathogenetic mechanisms. Along these lines, in a recent study we have provided evidence to indicate that aging promotes the growth of pre-neoplastic cells through alterations imposed on the tissue microenvironment, i.e. by generating an age-associated, neoplastic-prone tissue landscape. Similarly, it has been reported that aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors; it was proposed that cell competition may in fact drive the emergence of oncogenically altered cells in a background of age-induced decline in tissue fitness, in a process that has been referred to as ...
Our lab seeks to understand and control mechanical and other biophysical communication between cells and their surroundings. Three recent important recent directions for our work have been to: (1) Develop and apply biomaterial platforms and micro/nanoscale technologies to probe cellular force generation and migration; (2) Engineer new tissue-mimetic culture platforms for discovery, molecular analysis, and screening (often in collaboration with clinical colleagues); and (3) Explore mechanobiological signaling systems as targets for limiting the invasion of brain tumors and enhancing stem cell neurogenesis. Finally, we have recently begun to develop new materials based on cellular structural networks that may have broader applications in synthetic biology and soft materials science.. ...
Successful bone tissue engineering strategy relies on robust cell culturing technique as well as profitable cellular microenvironment. For the current clinical treatment, the expansion of cells in vitro has been a promising alternative method for the bone tissue engineering strategy. In order to enhance the culture efficiency, bioreactor systems are widely researched for the different clinical purposes. Currently, rotating wall bioreactors, biaxial bioreactors, perfusion bioreactors and spinner flask systems should be widely used in the experiments and each of them should has its own advantages and disadvanges. In this project, I would like to achieve the enhanced efficiency of human fetal mesenchymal stem cells (hfMSCs) by the parametric studies in biaxial bioreactor systems for the bone tissue engineering. The hypothesis include: 1). the osteogenesis of hfMSCs can be improved by mechano-induction effect; 2) chemical stimulation can safely enhance the proliferation and differentiation of ...
These dormant HSCs are thought to exist in a microenvironment termed the bone marrow niche. However, the location of the niche and the mechanism by which hibernation is maintained were almost completely unknown.. Professor Hiromitsu Nakauchi and his research group at the Institute of Medical Science have demonstrated for the first time that the bone marrow niche includes nonmyelinating Schwann cells, a type of glial nerve cell, and have described the mechanism by which hibernation is maintained. Until now it was thought that the nervous and hematopoietic systems were independent.. The research group determined that glial cells suppress HSC division by activating the protein TGF-β, and that HSCs in the bone marrow niche are affected by TGF-β through contact with glial cells.. In recent years, it has been pointed out that even leukemia stem cells exist in a state of hibernation in the bone marrow niche. This research result is important not only for understanding the mechanism of hematopoiesis ...
Sarkar, A, Parikh, N, Hearn, S. A., Fuller, M. T., Tazuke, S. I., Schulz, C. (2007) Antagonistic Roles of Rac and Rho in Organizing the Germ Cell Microenvironment. Current Biology, 17 (14). pp. 1253-1258. Sarkar, A. K., Luijten, M., Miyashima, S., Lenhard, M., Hashimoto, T., Nakajima, K., Scheres, B., Heidstra, R., Laux, T. (2007) Conserved factors regulate signalling in Arabidopsis thaliana shoot and root stem cell organizers. Nature, 446 (7137). pp. 811-4. ISSN 1476-4687 (Electronic) Sartorius, A., Henn, F. A. (2007) Deep brain stimulation of the lateral habenula in treatment resistant major depression. Med Hypotheses, 69 (6). pp. 1305-8. ISSN 0306-9877 (Print)0306-9877 (Linking) Sartorius, A., Mahlstedt, M. M., Vollmayr, B., Henn, F. A., Ende, G. (2007) Elevated spectroscopic glutamate/gamma-amino butyric acid in rats bred for learned helplessness. Neuroreport, 18 (14). pp. 1469-73. ISSN 0959-4965 (Print)0959-4965 (Linking) Sasaki, D., Kondo, S., Maeda, N., Gingeras, T. R., Hasegawa, Y., ...
Multiple myeloma (MM), a bone marrow-resident hematological malignancy of plasma cells, has remained largely incurable despite dramatic improvements in patient outcomes in the era of myeloma-targeted and immunomodulatory agents. It has recently become clear that T cells from MM patients are able to recognize and eliminate myeloma, although this is subverted in the majority of patients who eventually succumb to progressive disease. T cell exhaustion and a suppressive bone marrow microenvironment have been implicated in disease progression, and once these are established, immunotherapy appears largely ineffective. Autologous stem cell transplantation (ASCT) is a standard of care in eligible patients and results in immune effects beyond cytoreduction, including lymphodepletion, T cell priming via immunogenic cell death, and inflammation; all occur within the context of a disrupted bone marrow microenvironment. Recent studies suggest that ASCT reestablishes immune equilibrium and thus represents a ...
Age-related changes in the hematopoietic compartment are primarily attributed to cell-intrinsic alterations in hematopoietic stem cells (HSCs); however, the contribution of the aged microenvironment has not been adequately evaluated. Understanding the role of the bone marrow (BM) microenvironment in supporting HSC function may prove to be beneficial in treating age-related functional hematopoietic decline. Here, we determined that aging of endothelial cells (ECs), a critical component of the BM microenvironment, was sufficient to drive hematopoietic aging phenotypes in young HSCs. We used an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating activity of young HSCs and impart a myeloid bias. Conversely, young ECs restored the repopulating capacity of aged HSCs but were unable to reverse the intrinsic myeloid bias. Infusion of young, ...
Age-related changes in the hematopoietic compartment are primarily attributed to cell-intrinsic alterations in hematopoietic stem cells (HSCs); however, the contribution of the aged microenvironment has not been adequately evaluated. Understanding the role of the bone marrow (BM) microenvironment in supporting HSC function may prove to be beneficial in treating age-related functional hematopoietic decline. Here, we determined that aging of endothelial cells (ECs), a critical component of the BM microenvironment, was sufficient to drive hematopoietic aging phenotypes in young HSCs. We used an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating activity of young HSCs and impart a myeloid bias. Conversely, young ECs restored the repopulating capacity of aged HSCs but were unable to reverse the intrinsic myeloid bias. Infusion of young, ...
Age-related changes in the hematopoietic compartment are primarily attributed to cell-intrinsic alterations in hematopoietic stem cells (HSCs); however, the contribution of the aged microenvironment has not been adequately evaluated. Understanding the role of the bone marrow (BM) microenvironment in supporting HSC function may prove to be beneficial in treating age-related functional hematopoietic decline. Here, we determined that aging of endothelial cells (ECs), a critical component of the BM microenvironment, was sufficient to drive hematopoietic aging phenotypes in young HSCs. We used an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating activity of young HSCs and impart a myeloid bias. Conversely, young ECs restored the repopulating capacity of aged HSCs but were unable to reverse the intrinsic myeloid bias. Infusion of young, ...
Age-related changes in the hematopoietic compartment are primarily attributed to cell-intrinsic alterations in hematopoietic stem cells (HSCs); however, the contribution of the aged microenvironment has not been adequately evaluated. Understanding the role of the bone marrow (BM) microenvironment in supporting HSC function may prove to be beneficial in treating age-related functional hematopoietic decline. Here, we determined that aging of endothelial cells (ECs), a critical component of the BM microenvironment, was sufficient to drive hematopoietic aging phenotypes in young HSCs. We used an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating activity of young HSCs and impart a myeloid bias. Conversely, young ECs restored the repopulating capacity of aged HSCs but were unable to reverse the intrinsic myeloid bias. Infusion of young, ...
Zeilinger, Sylvia wed Thomas Zeilinger. CHJ 27 Apr 1939. Z453 Zelinder, Richard c Elizabeth Lee. Z455. Zillman, Pauls SON, nee 14 Apr KC; m Marybelle Burch. CHJ 17 Apr 1944. Zollman, Mabel Severns, b 28 Aug 1884 nr Ulysses, Butler co, Ne; d Jan 1974; wed 27 May 1908 Frank Zollman; i Bkt. B 1974. Zollman, Mary J., b 03 Sep 1832 nr Columbus, Oh; d 12 Nov 1917; wed 05 Apr 1853 Alfred Boyd, d 01 Jan 1894, 4 ch; wed Amos Cox, d 12 Jul 1898. SW 15 Nov 1917 (2) Zollman, Albert c Lucille Farshaw Zollman, Bertha (Ms) of Rockbridge co, Va vis bro Reece and aunt Mrs John Supinger. BFP 25 Jun 1908 Zollman, Bertha c John Richard Supinger Zollman, Frank Alexander, b 30 Jul 1886 nr Lxtn, Va; d 14 May 1969 MtAyr hosp; wed 27 May 1908 Mable Severns at Claremore, Oh, 6 ch; f William Zollman; m Ida Cunningham; i Bkt. B 22 May 1969 Zolman, Frank c Mabel Severns Zollman, Frank c Ora Alice Bell Zellman, Harry, d Grant City?; f Reese Zellman; i Bkt. B 25 Mar 1937 Zollman, Lenore c Everett King Zollman, Lucile, b 14 ...
The tissue microenvironment is dynamically modeled with portal to centrilobular blood flow. Each agent autonomously processes inputs from the microenvironment using biological rules to generate a response. The biological rules and agent responses are derived from prior knowledge and experimental data on hepatocyte (HC) and Kupffer cell (KC) physiology. This includes a range of cellular inputs leading to HC adaptation, HC injury, KC migration, HC necrosis, apoptosis and proliferation. The v-Liver system enables in silico investigation of liver lesions as emergent tissue-level outcomes. We demonstrate the use of the v-Liver to analyze the formation of pre-neoplastic and neoplastic lesions by: (a) selecting non-genotoxic carcinogens, (b) encoding knowledge about carcinogenic MoA as biological rules, and (c) calibrating quantitative agent responses using cell-level in vitro data from ToxCast and ex vivo tissue samples. ...
Soluble molecules (Cytokines) that are found in BM microenvironment such as IL6, IGF1, VEGF, TNF-α, and SDF-1α trigger RAS and activate two different signalling pathways: 1) RAF/MEK/MAPK inducing proliferation of MM cells and 2) PI3K/AKT which prevents apoptosis induced by both ionizing radiation and drugs (see below). The RAS pathway in turn activates NF-KB and endogenous production of IL6. Cytokines such as IL-6 and IL-21 can trigger a third route, the JAK/STAT cascade, that also prevents apoptosis with overexpression of Bcl-XL and inhibition of CD95 (FAS) induced apoptosis. In addition, TNF triggers other transduction signalling routes such as NF-kB and JNK/Ap-1. VEGF and SDF-1α are important in migration. The migration induced by VEGF is mediated by PI3K, which induces downstream activation of PKC. ...
FederalGrants.com opportunity listing for the Interactions Between Stem and Progenitor Cells and the Microenvironment in Vivo (R01) federal grant. Includes information on eligibility, deadlines, requirements, and guidelines.
The eigenvalue assignment problem involves finding a state feedback vector k such that the eigenvalues of A - bkT are in the desired locations. The authors consider the case in which the system is not completely controllable and the solution for the feedback gain vector k is not unique. The set of p.... Full description. ...
Combat or Surveillance? Why morphology and spatial relationships are key to describe the functional state of the immune system. Understanding the cellular and
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TY - JOUR. T1 - NADPH oxidase 2 regulates bone marrow microenvironment following hindlimb ischemia. T2 - Role in reparative mobilization of progenitor cells. AU - Urao, Norifumi. AU - McKinney, Ronald D.. AU - Fukai, Tohru. AU - Ushio-Fukai, Masuko. PY - 2012/5/1. Y1 - 2012/5/1. N2 - Bone marrow (BM) microenvironment, which is regulated by hypoxia and proteolytic enzymes, is crucial for stem/progenitor cell function and mobilization involved in postnatal neovascularization. We demonstrated that NADPH oxidase 2 (Nox2)-derived reactive oxygen species (ROS) are involved in postischemic mobilization of BM cells and revascularization. However, role of Nox2 in regulating BM microenvironment in response to ischemic injury remains unknown. Here, we show that hindlimb ischemia of mice increases ROS production in both the endosteal and central region of BM tissue in situ, which is almost completely abolished in Nox2 knockout (KO) mice. This Nox2-dependent ROS production is mainly derived from Gr-1+myeloid ...
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The spread of multiple myeloma (MM) involves (re)circulation into the peripheral blood and (re)entrance or homing of MM cells into new sites of the BM. bloodstream and home into new BM niches. The conversation of MM cells with the BM microenvironment plays a crucial role in MM progression and dissemination.3C5 We showed previously that disrupting the interaction between MM cells and the BM microenvironment through inhibition of CXCR4 or its downstream signaling altered MM cell trafficking, including inhibition of MM cell homing to the BM, decreased MM proliferation, tumor progression, and drug resistance.6C8 However, the driving force that leads MM cells to egress from one site of the BM to disseminate and home to the next BM site is still not fully understood. Regions of low oxygenation (hypoxia) arise in tumors because of rapid cell division and aberrant blood vessel formation.9 Several studies have indicated that the hypoxic microenvironment contributes to cancer progression by activating ...
Sigma-Aldrich offers abstracts and full-text articles by [Peng Zhang, Zizhen Chen, Rong Li, Ying Guo, Hui Shi, Jie Bai, Hui Yang, Mengyao Sheng, Zhaomin Li, Zhuo Li, Jianping Li, Shi Chen, Weiping Yuan, Tao Cheng, Mingjiang Xu, Yuan Zhou, Feng-Chun Yang].
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Tumor cells have to overcome challenges in the host tissue microenvironment to metastasize successfully to distant organs. In a recent Nature study, a genome-wide functional screen demonstrated that deficiency of the sphingosine-1-phoshate (S1P) transporter gene Spns2 in endothelium increased immune-mediated cell killing by T cells and natural killer (NK) cells, thereby suppressing metastatic colonization.
A class action against the microenvironment: do cancer cells cooperate in metastasis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
S2M Science to Medicine Seminar Series - Metabolic Conditions in the Tumor Microenvironment Promote the Development of Myeloid Derived Suppressor Cells - New Therapeutic ...
American-made, FDA-approved, and BPA-free: this high-density white polyethylene pail has a 2-gallon capacity, secure lid and strong handle, so its a happy home for your bag of Just Protein, making it easy to access and keeping it out of harms way. Its also a perfect container for your gym chalk. And you can even toss some ice in one, add your favorite cold beverages, and take them for a ride. No. 1261-BKT (2 lb.) $11.95. ...
Tumors are organ-like structures composed of different cell types whose interactions are required to promote their maintenance, growth and metastasis.
Dr. John M. Collins received his PhD degree in Bioengineering from the University of Illinois at Chicago where he worked in the Physiology and Biophysics department in Professor Brenda Russells laboratory. His research focused on understanding the physical and chemical cues of the cellular microenvironment and their impact on stem cell function. He is a Senior Applications Scientist at NanoInk, Inc. and has been responsible for the development of cell based assays using tip-based lithography. He is currently developing methods to enable single cell manipulation via sub-cellular scaled features of various biomolecules. ...
The molecular mechanisms by which iron is physiologically transported trough the cellular membranes are still only partially understood. Several studies indicate that a reduction step of ferric iron to ferrous is necessary, both in the case of transferrin-mediated and transferrin-independent iron uptake. Recent studies from our laboratory described gamma-glutamyltransferase activity (GGT) as a factor capable to effect iron reduction in the cell microenvironment. GGT is located on the outer aspect of plasma membrane of most cell types, and is often expressed at high levels in malignant tumors and their metastases. The present study was aimed at verifying the possibility that GGT-mediated iron reduction may participate in the process of cellular iron uptake. Four distinct human tumor cell lines, exhibiting different levels of GGT activity, were studied. The uptake of transferrin-bound iron was investigated by using 55Fe-loaded transferrin, as well as by monitoring fluorimetrically the intracellular iron
The tumor immune microenvironment (TIME) is the cellular environment in which tumors exist. This includes: surrounding blood vessels, immune cells, fibroblasts, bone marrow-derived inflammatory cells, lymphocytes, signaling molecules, immune checkpoint proteins and the extracellular matrix (ECM). The TIME plays a critical role in cancer progression and regulation. Tumors can influence the microenvironment by releasing extracellular signals, promoting tumor angiogenesis and inducing peripheral immune tolerance, while the immune cells in the microenvironment can affect the growth and evolution of cancerous cells. The molecules and cells in the TIME influence disease outcome by altering the balance of suppressive versus cytotoxic responses in the vicinity of the tumor. Having a better understanding of the tumor immune microenvironment will pave the way for identifying new targets for immunotherapies that promote cancer elimination.
Biobanking 2018 will bring together the worlds leading experts in biorepositories, policy makers, and scientific actors to strengthen knowledge in biobanking as well as explore future advances in the field of biosampling for precision medicine, drug development and health research.
Rapidly growing tumors and tumor metastases are exposed to ongoing hypoxic conditions in the tissue microenvironment. Hypoxia, in turn, has a profound influence on tumor pathophysiology. The survival of the tumor and its aggressiveness depend on its ability to respond quickly to hypoxia cues. Cells undergo a variety of biological responses when placed under hypoxic conditions, including activation of signaling pathways that regulate proliferation, angiogenesis, and death (1-3). Cancer cells have adapted these pathways, allowing tumors to survive and even grow under hypoxic conditions (2). Although tissue hypoxia is a well-documented phenomenon, our understanding of the response of tumor cells to hypoxia is far from being complete. The response to hypoxia may be divided into two mechanisms: the first one sensing the level of oxygen and the second one activating the hypoxia-induced genes, some of which contribute to tumor angiogenesis. A large body of evidence accumulated with regard to the role ...
In this issue, Singh et al. set out to decipher the mechanism by which CD26 relays G-CSF-induced HSPC mobilization (16, 17). This was achieved by performing sophisticated experiments to determine the extent to which CD26 expression by hematopoietic cells or by stromal cells in the BM microenvironment is essential for the stem cell mobilization process (1). Contrary to the prior hypothesis suggesting that CXCL12 cleavage by hematopoietic CD26 promotes HSPC migration out of the BM, Singh and colleagues showed that hematopoietic expression of CD26 is not essential for HSPC mobilization. Moreover, CD26 expression was reduced in mobilized HSPCs, and deletion of CD26 in murine HSPCs did not alter HSPC mobilization in a WT environment (1). These unexpected results align with recent reports indicating that CD26 truncates inflammatory cytokines into a nonactive form (18, 19) and, as such, may interfere with the hematopoietic response during alert and stress conditions. However, CD26 expression by niche ...
Yorba Linda, CA (PRWEB) September 07, 2016 -- Biomedical research has gravitated towards 3D cell culture platforms due to their better representation of in
If cellular automata rules have not been formulated from the first principles of the subject, then such a model may have a weak ... Ch'ng, E. (2012) Macro and Micro Environment for Diversity of Behaviour in Artificial Life Simulation, Artificial Life Session ... The idea intrigued von Neumann, who drew it up-creating the first of the devices later termed cellular automata. Another ... The deterministic logical cellular automata are necessary but not sufficient condition of a white-box model. The second ...
... precise control of cellular microenvironment, as well as controlled integration of cells into appropriate multi-cellular ... Microfluidics have been applied in these technologies to better mimic the in vivo microenvironment with patterned topographic ... Flaim, Christopher J; Chien, Shu; Bhatia, Sangeeta N (2005). "An extracellular matrix microarray for probing cellular ... Rosenthal, Adam; Macdonald, Alice; Voldman, Joel (2007). "Cell patterning chip for controlling the stem cell microenvironment ...
"The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... "Lysosomal-mediated degradation of apoptotic thymocytes within thymic nurse cells." Cellular immunology 197.2 (1999): 108-115. ... "The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... "The thymus microenvironment in regulating thymocyte differentiation." Cell adhesion & migration 4.3 (2010): 382-390. Nakagawa, ...
in Symbiosis with Didemnid Ascidians". In Seckbach, J. Cellular origin and life in extreme habitats (PDF). 3: Symbiosis, ... Kühl, Micahel; Larkum, Anthony W.D. (2002). "The Microenvironment and Photosynthetic Performance of Prochloron sp. ...
If cellular automata rules have not been formulated from the first principles of the subject, then such a model may have a weak ... Macro and Micro Environment Simon, Herbert A. The sciences of the artificial. MIT press, 1996. Situngkir, Hokky (2004). " ... The deterministic logical cellular automata are necessary but not sufficient condition of a white-box model. The second ... The idea intrigued von Neumann, who drew it up-creating the first of the devices later termed cellular automata. Another ...
... merging microenvironments, niches, and cellular transformation". European Journal of Dermatology. doi:10.1684/ejd.2011.1267. ...
Stem cells are also tightly regulated by their respective cellular microenvironment or niche; PcG function can be inhibited by ... Cellular and Molecular Life Sciences". CMLS. 71 (2): 257-69. doi:10.1007/s00018-013-1426-x. Schuringa, JJ; Vellenga, E (2010 ... Hence, these data present a model in which c-Myc and Bmi-1 alter cellular apoptosis via cell cycle regulator genes. Conversely ... In mammals, the composition of PRC1 is much more diverse and varies depending on the cellular context. All PRC1 complexes ...
Do, J. T.; Han, D. W.; Gentile, L; Sobek-Klocke, I; Stehling, M; Lee, H. T.; Schöler, H. R. (2007). "Erasure of cellular memory ... Lin, Ji; Mei-rong Li, Dong-dong Ti; Wei-dong, Han (2013). "Microenvironment-evoked cell lineage conversion: Shifting the focus ... For example, cellular therapy employing genetic modification with Pim-1 kinase (a downstream effector of Akt, which positively ... 2013). "Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived ...
This approach depends more on the field control and less on the cellular microenvironment. In vitro and in vivo (on mice) ... "Lipoic acid affects cellular migration into the central nervous system and stabilizes blood-brain barrier integrity". J. ...
The Centre focuses on cellular matrix microenvironment, matrix immunobiology, and mechanobiology of the matrix. As of 2016[ ...
This micro-environment acidification can lead to cellular stress, which would lead to autophagy. Autophagy is activated in ... and during certain cellular developmental stages, such as during cellular differentiation of red blood cells. Organelles and ... Additionally, in the microenvironment of cancer cells, there is an increase in hypoxia-inducible transcription factor 1-alpha ( ... Disorders in energy creation by mitochondria can cause cellular degeneration, like those seen in the substantia nigra. ...
Therefore, this classification may be paraphyletic because cellular life might have evolved from non-cellular life, or ... Cells have evolved methods to perceive and respond to their microenvironment, thereby enhancing their adaptability. Cell ... The individual members of a colony are capable of surviving on their own, whereas the members of a true multi-cellular organism ... As microbiology, molecular biology and virology developed, non-cellular reproducing agents were discovered, such as viruses and ...
Therefore, it is necessary to understand the microenvironment, the nature of the biological forces in this microenvironment, ... In some processes, the cells are encapsulated in cellular spheroids 500μm in diameter. This aggregation of cells does not ... It demands a deeper understanding of how embryonic tissues mechanisms develop as well as the microenvironment surrounded to ... Biomimicry requires duplication of the shape, framework, and the microenvironment of the organs and tissues. The application of ...
Cellular uptake of certain small molecule drugs is also increased. Very high temperatures, above 50 °C (122 °F), are used for ... However, the vascular disorganization of a solid tumor results in an unfavorable microenvironment inside tumors. Consequently, ...
... and C6 gene products within the human thymic cellular microenvironment". In Vivo. 14 (3): 419-24. PMID 10904875. Giannola DM, ... Molecular and Cellular Biology. 21 (21): 7509-22. doi:10.1128/MCB.21.21.7509-7522.2001. PMC 99922 . PMID 11585930. Kömüves LG, ...
The gap junction had been shown to create a micro-environment between the two cells in the extra-cellular space or "gap". This ... Cellular membranes in development. New York: Academic Press. OCLC 261587041.. [page needed] ... Tissues in this section have well known functions observed to be coordinated by gap junctions with inter-cellular signaling ... which was originally thought to form inter-cellular channels (with an amino acid sequence similar to innexins[13]), in fact ...
Composed of cellular membranes with multiple adhesive proteins on their surface, exosomes are known to specialize in cell-cell ... suggesting that tumor cells adapt to a hypoxic microenvironment by secreting exosomes to stimulate angiogenesis or facilitate ... In that study, the differences in cellular and exosomal mRNA and miRNA content was described, as well as the functionality of ... 1] Increasingly, exosomes are being recognized as potential therapeutics as they have the ability to elicit potent cellular ...
... a tumour supportive micro-environment through the production of matri-cellular proteins.The up regulation of matri-cellular ... The cellular response to hypoxia is mediated by the transcription factor HIF-1, which is a heterodimer protein composed of α ... Cellular extensions also enable the cells to sense their environment. Following inflammation or injury to the pancreas, ... Matri-cellular proteins may therefore directly contribute to the development of pancreatic cancer through stimulating cancer ...
2010). "Impact of interactions of cellular components of the bone marrow microenvironment on hematopoietic stem and progenitor ... The landscape of the HSC niche in the bone marrow is constantly changing, and the acellular factors, as much as the cellular ... 2009). "Altered cellular dynamics and endosteal location of aged early hematopoietic progenitor cells revealed by time-lapse ... Martin, MA, Bhatia & M (2005). "Analysis of the human fetal liver hematopoietic microenvironment". Stem Cells Dev. 14 (5): 493- ...
Kumari, I; Ahmed, M; Akhter, Y (2017). "Evolution of catalytic microenvironment governs substrate and product diversity in ... Cellular and Molecular Life Sciences. 9 (10): 357-367. doi:10.1007/BF02167631. ...
Cellular level: describe how geometrical, biochemical or mechanical cues can be integrated at the cellular level to influence ... These experiments rely on physical approaches to change and control the cell microenvironment in a precise and refined manner. ... Microscopy: The DEEP laboratory depends greatly on optical imaging and image analysis to measure the dynamics of cellular ... we obtain quantitative descriptions of the cellular properties. Emphasis is put on studying membrane remodeling. ...
Reactive oxygen species plays a role in cellular transformation. Various PKC isoforms were implicated in p47phox/Nox1 axis ... Lactic acid, a tumor derived metabolite, altering pH of tumor microenvironment enhances sphere formation from blebbishields ... Blebbishields in turn fuse to each other and generate cancer stem cell spheres/cellular transformation, essentially shifting ... Blebbishield emergency program: an apoptotic route to cellular transformation. Cell Death Differ. 2016 In Press. Jinesh GG, & ...
Various integrin receptors monitor the mechanical properties of the cellular microenvironment and can influence and initiate ... Podosomes are thought to be intimately connected to cellular motility within tissue microenvironments through coordinating ... immune systems do not manifest podosome formations and demonstrate defects in cellular movement within tissue microenvironments ... Their primary purpose is connected to cellular motility and invasion; therefore, they serve as both sites of attachment and ...
Mechanisms of control via regulation of extracellular matrix proteins, cellular surface markers, cellular adhesion molecules, ... Landscaper genes encode gene products that control the microenvironment in which cells grow. Growth of cells depends both on ... Cellular senescence, also encoded by a gatekeeper gene, is arrest of the cell cycle in the G1 phase. Qualitative differences ... This is because the products of gatekeeper genes are directly involved in coding for cellular growth and proliferation. It is ...
cellular response to drug. • cell cycle arrest. • learning or memory. • cellular copper ion homeostasis. • cellular response to ... These interfaces allow specific sets of cells to communicate based on level of expression and the surrounding microenvironment ... Cellular component. • mitochondrial outer membrane. • membrane. • mitochondrion. • integral component of membrane. • cytoplasm ... CellularEdit. Varying expression of PrP through the cell cycle has led to speculation on involvement in development. A wide ...
This is due to the accumulation of oxidative damage to DNA by aging and cellular metabolic activity and the shortening of ... Lefebvre JS, Maue AC, Eaton SM, Lanthier PA, Tighe M, Haynes L (2012). "The aged microenvironment contributes to the age- ... Cambier, J. (2005). "Immunosenescence: a problem of lymphopoiesis, homeostasis, microenvironment, and signaling". Immunological ...

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