Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Stem Cell Niche: A particular zone of tissue composed of a specialized microenvironment where stem cells are retained in a undifferentiated, self-renewable state.Hematopoietic Stem Cell Mobilization: The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Mice, Inbred C57BLColony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Blood Cells: The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.Multipotent Stem Cells: Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Fetal Blood: Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Graft vs Host Disease: The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.Cell SeparationCell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Transplantation Conditioning: Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Neural Stem Cells: Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.Hematopoietic System: The blood-making organs and tissues, principally the bone marrow and lymph nodes.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Induced Pluripotent Stem Cells: Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.Transplantation, Autologous: Transplantation of an individual's own tissue from one site to another site.Hematologic Neoplasms: Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.Mesonephros: One of a pair of excretory organs (mesonephroi) which grows caudally to the first pair (PRONEPHROI) during development. Mesonephroi are the permanent kidneys in adult amphibians and fish. In higher vertebrates, proneprhoi and most of mesonephroi degenerate with the appearance of metanephroi. The remaining ducts become WOLFFIAN DUCTS.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Time Factors: Elements of limited time intervals, contributing to particular results or situations.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Fetal Stem Cells: Cells derived from a FETUS that retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Homeodomain Proteins: Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).Proto-Oncogene Proteins c-kit: A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.Transplantation Chimera: An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Mice, Inbred NOD: A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cord Blood Stem Cell Transplantation: Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Lentivirus: A genus of the family RETROVIRIDAE consisting of non-oncogenic retroviruses that produce multi-organ diseases characterized by long incubation periods and persistent infection. Lentiviruses are unique in that they contain open reading frames (ORFs) between the pol and env genes and in the 3' env region. Five serogroups are recognized, reflecting the mammalian hosts with which they are associated. HIV-1 is the type species.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Whole-Body Irradiation: Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Graft Survival: The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Myeloid Progenitor Cells: Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Neoplastic Stem Cells: Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.Blood Cell Count: The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.Granulocyte Colony-Stimulating Factor: A glycoprotein of MW 25 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.Regeneration: The physiological renewal, repair, or replacement of tissue.Granulocytes: Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Chimerism: The occurrence in an individual of two or more cell populations of different chromosomal constitutions, derived from different individuals. This contrasts with MOSAICISM in which the different cell populations are derived from a single individual.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Lymphopoiesis: Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Stromal Cells: Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Hematopoiesis, Extramedullary: The formation and development of blood cells outside the BONE MARROW, as in the SPLEEN; LIVER; or LYMPH NODES.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Tretinoin: An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).Peripheral Blood Stem Cell Transplantation: Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Tissue Donors: Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Basic Helix-Loop-Helix Transcription Factors: A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Radiation Chimera: An organism whose body contains cell populations of different genotypes as a result of the TRANSPLANTATION of donor cells after sufficient ionizing radiation to destroy the mature recipient's cells which would otherwise reject the donor cells.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Antigens, Differentiation: Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.Receptors, Notch: A family of conserved cell surface receptors that contain EPIDERMAL GROWTH FACTOR repeats in their extracellular domain and ANKYRIN repeats in their cytoplasmic domains. The cytoplasmic domain of notch receptors is released upon ligand binding and translocates to the CELL NUCLEUS where it acts as transcription factor.Thrombopoietin: A humoral factor that stimulates the production of thrombocytes (BLOOD PLATELETS). Thrombopoietin stimulates the proliferation of bone marrow MEGAKARYOCYTES and their release of blood platelets. The process is called THROMBOPOIESIS.Fetus: The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.Myeloid Cells: The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).Antigens, CD38: A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Antigens, Ly: A group of lymphocyte surface antigens located on mouse LYMPHOCYTES. Specific Ly antigens are useful markers for distinguishing subpopulations of lymphocytes.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Myelopoiesis: Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Immunophenotyping: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Yolk Sac: The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Spleen: An encapsulated lymphatic organ through which venous blood filters.Epigenesis, Genetic: A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.Megakaryocytes: Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Antigens, Thy-1: A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.Busulfan: An alkylating agent having a selective immunosuppressive effect on BONE MARROW. It has been used in the palliative treatment of chronic myeloid leukemia (MYELOID LEUKEMIA, CHRONIC), but although symptomatic relief is provided, no permanent remission is brought about. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), busulfan is listed as a known carcinogen.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Histocompatibility Testing: Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)Thymus Gland: A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Gonads: The gamete-producing glands, OVARY or TESTIS.Erythropoiesis: The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.Lymphoid Progenitor Cells: Stem cells from which B-LYMPHOCYTES; T-LYMPHOCYTES; NATURAL KILLER CELLS; and some DENDRITIC CELLS derive.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Hematologic Diseases: Disorders of the blood and blood forming tissues.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.GATA2 Transcription Factor: An essential GATA transcription factor that is expressed primarily in HEMATOPOIETIC STEM CELLS.Octamer Transcription Factor-3: An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION.Chemokine CXCL12: A CXC chemokine that is chemotactic for T-LYMPHOCYTES and MONOCYTES. It has specificity for CXCR4 RECEPTORS. Two isoforms of CXCL12 are produced by alternative mRNA splicing.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Transplantation, Heterologous: Transplantation between animals of different species.Histocompatibility: The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.Plant Stems: Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)Core Binding Factor Alpha 2 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Mesoderm: The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.Receptor, Notch1: A notch receptor that interacts with a variety of ligands and regulates SIGNAL TRANSDUCTION PATHWAYS for multiple cellular processes. It is widely expressed during EMBRYOGENESIS and is essential for EMBRYONIC DEVELOPMENT.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Mice, Mutant Strains: Mice bearing mutant genes which are phenotypically expressed in the animals.Severe Combined Immunodeficiency: Group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. It is inherited as an X-linked or autosomal recessive defect. Mutations occurring in many different genes cause human Severe Combined Immunodeficiency (SCID).Antigens, CD45: High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.Myelodysplastic Syndromes: Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Erythroid Precursor Cells: The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.Mice, Congenic: Mouse strains constructed to possess identical genotypes except for a difference at a single gene locus.Mice, Inbred BALB CCell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Hematopoietic Cell Growth Factors: These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.Cell Transplantation: Transference of cells within an individual, between individuals of the same species, or between individuals of different species.Cell Aging: The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Retrospective Studies: Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.HL-60 Cells: A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)Cellular Microenvironment: Local surroundings with which cells interact by processing various chemical and physical signals, and by contributing their own effects to this environment.Interleukin Receptor Common gamma Subunit: An interleukin receptor subunit that was originally discovered as a component of the INTERLEUKIN 2 RECEPTOR. It was subsequently found to be a component of several other receptors including the INTERLEUKIN 4 RECEPTOR, the INTERLEUKIN 7 RECEPTOR, the INTERLEUKIN-9 RECEPTOR, the INTERLEUKIN-15 RECEPTOR, and the INTERLEUKIN-21 RECEPTOR. Mutations in the gene for the interleukin receptor common gamma chain have been associated with X-LINKED COMBINED IMMUNODEFICIENCY DISEASES.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Leukemia, Myelogenous, Chronic, BCR-ABL Positive: Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.Cell Line, Tumor: A cell line derived from cultured tumor cells.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Cell Adhesion: Adherence of cells to surfaces or to other cells.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Graft vs Leukemia Effect: Immunological rejection of leukemia cells following bone marrow transplantation.Receptors, Thrombopoietin: Cell surface receptors that are specific for THROMBOPOIETIN. They signal through interaction with JANUS KINASES such as JANUS KINASE 2.Myeloablative Agonists: Agents that destroy bone marrow activity. They are used to prepare patients for BONE MARROW TRANSPLANTATION or STEM CELL TRANSPLANTATION.Cell Dedifferentiation: A reverse developmental process in which terminally differentiated cells with specialized functions revert back to a less differentiated stage within their own CELL LINEAGE.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Nerve Tissue ProteinsGene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Wnt Proteins: Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.Erythroid Cells: The series of cells in the red blood cell lineage at various stages of differentiation.Cell- and Tissue-Based Therapy: Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.Receptors, CXCR4: CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.Morphogenesis: The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.Embryonic Development: Morphological and physiological development of EMBRYOS.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.ADP-ribosyl Cyclase: A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.Muscle Development: Developmental events leading to the formation of adult muscular system, which includes differentiation of the various types of muscle cell precursors, migration of myoblasts, activation of myogenesis and development of muscle anchorage.Regenerative Medicine: A field of medicine concerned with developing and using strategies aimed at repair or replacement of damaged, diseased, or metabolically deficient organs, tissues, and cells via TISSUE ENGINEERING; CELL TRANSPLANTATION; and ARTIFICIAL ORGANS and BIOARTIFICIAL ORGANS and tissues.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Myeloproliferative Disorders: Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.Chimera: An individual that contains cell populations derived from different zygotes.

*Nucleated red blood cell

Blood cell lineage Hematopoiesis Erythropoiesis Haematopoiesis Hematopoietic stem cell Hartenstein, V (2006). "Blood cells and ... and the nucleus is ejected as a normal part of cellular differentiation before the cell is released into the bloodstream. Thus ... all vertebrate organisms have hemoglobin-containing cells in their blood and all of these red blood cells contain a nucleus. ... In contrast, a nucleated red blood cell (NRBC), also known by several other names, is a mammalian RBC that contains a cell ...

*MiR-155

Hematopoiesis is defined as the formation and development of blood cells, all of which are derived from hematopoietic stem- ... is expressed in hematopoietic cells it was hypothesized that this miRNA plays a critical role in these cellular differentiation ... Mayani H (Mar 2010). "Biological differences between neonatal and adult human hematopoietic stem/progenitor cells". Stem Cells ... "CD34+ hematopoietic stem-progenitor cell microRNA expression and function: a circuit diagram of differentiation control". ...

*Cellular differentiation

Hematopoietic stem cells (adult stem cells) from the bone marrow that give rise to red blood cells, white blood cells, and ... adult stem cells) from the bone marrow that give rise to stromal cells, fat cells, and types of bone cells Epithelial stem ... Examples of stem and progenitor cells include:[citation needed] Radial glial cells (embryonic neural stem cells) that give rise ... The transduction of the stem cells into these cells types is not directed solely by chemokine cues and cell to cell signaling. ...

*Haematopoiesis

... is the formation of blood cellular components. All cellular blood components are derived from haematopoietic stem cells. In a ... a stem cell factor, subdivides the population into groups exhibiting variable rates of cellular differentiation. For example, ... All blood cells are divided into three lineages. Erythroid cells are the oxygen carrying red blood cells. Both reticulocytes ... For the stem cells and other undifferentiated blood cells in the bone marrow, the determination is generally explained by the ...

*ABCG2

... and at the blood-testis barrier, the blood-brain barrier, and the membranes of hematopoietic progenitor and other stem cells. ... ABCG2 has also been designated as CDw338 (cluster of differentiation w338). The membrane-associated protein encoded by this ... ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct ... Abbott BL (2006). "ABCG2 (BCRP): a cytoprotectant in normal and malignant stem cells". Clin Adv Hematol Oncol. 4 (1): 63-72. ...

*Endothelial stem cell

... form the blood circulatory system. Hematopoietic stem cells can of course undergo self-renewal, and are multipotent cells that ... ESCs have the characteristic properties of a stem cell: self-renewal and differentiation. These parent stem cells, ESCs, give ... These cell types accelerate the healing process and prevent further complications such as hypoxia by gathering the cellular ... It is characteristic of hematopoietic stem cells, as well as muscle stem cells. The two lineages arising from the EPC and the ...

*NOV (gene)

It is essential for self-renewal of CD34+ hematopoietic stem cells from umbilical cord blood. NOV can bind BMP2 and inhibit its ... NOV regulates multiple cellular activities including cell adhesion, migration, proliferation, differentiation, and survival. It ... functions as a regulator of human hematopoietic stem or progenitor cells". Science. 316 (5824): 590-3. doi:10.1126/science. ... stimulated secretion of CCN3 is involved in growth and differentiation regulation". Blood. 108 (5): 1716-23. doi:10.1182/blood- ...

*Stem cell laws

... multi-cellular organisms. A common example of a stem cell is the hematopoietic stem cell (HSC) which are multipotent stem cells ... that give rise to cells of the blood lineage. In contrast to multipotent stem cells, embryonic stem cells are pluripotent and ... The laws surrounding the two differ because while both have similar capacities in differentiation, their modes of derivation ... "Stem Cell Research in Spain: If Only They Were Windmills …". Cell Stem Cell. 4 (6): 483-6. doi:10.1016/j.stem.2009.05.016. PMID ...

*CD117

... blood) progenitors in the bone marrow. To be specific, hematopoietic stem cells (HSC), multipotent progenitors (MPP), and ... Signaling through CD117 plays a role in cell survival, proliferation, and differentiation. Hematopoietic progenitor cells are ... KIT was first described by the German biochemist Axel Ullrich in 1987 as the cellular homolog of the feline sarcoma viral ... Mobilization is used clinically as a source of hematopoietic stem cells for hematopoietic stem cell transplantation (HSCT). ...

*CLEC11A

... stem cell factor and rhIL-4 stimulate differentiation and proliferation of CD3+ cells from umbilical cord blood and CD3+ cells ... "Serum stem cell growth factor for monitoring hematopoietic recovery following stem cell transplantation". Bone Marrow ... enhance FcepsilonR1 expression on fetal liver-derived mast cells in the presence of rhIL-4". Cellular Immunology. 226 (1): 30-6 ... "In vitro differentiation of endothelial cells from AC133-positive progenitor cells". Blood. 95 (10): 3106-12. PMID 10807776. ...

*Mesenchymal stem cell

These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell ... "Non-hematopoietic bone marrow stem cells: Molecular control of expansion and differentiation". Experimental Cell Research. ... The International Society for Cellular Therapy (ISCT) has proposed a set of standards to define MSCs. A cell can be classified ... Mesenchymal stem cells, but not red blood cells or haematopoetic progenitors, are adherent to tissue culture plastic within 24 ...

*Cell therapy

"Stem cell c-KIT and HOXB4 genes: Critical roles and mechanisms in self-renewal, proliferation and differentiation". Stem Cells ... Cell therapy can be defined as therapy in which cellular material is injected into a patient. There are two branches of cell ... Wilson A, Trumpp A (2006). "Bone-marrow haematopoietic stem-cell niches". Nature Immunology. 6: 93-106. doi:10.1038/nri1779. ... In addition to BM derived HSCs, the use of alternative sources such as umbilical cord blood (UCB) and peripheral blood stem ...

*Martin Zenke

These conditional oncogenes were used to study differentiation of hematopoietic progenitor cells into red blood cells and ... Current work also includes studies on embryonic stem cells and reprogramming of somatic cells towards pluripotency (cellular ... In following up on this work the Zenke laboratory now focuses on stem cells, in particular on hematopoietic stem cells and ... Professor for Cell Biology and scientist, who is conducting research on stem cells and biomedical engineering. Martin Zenke ...

*T cell

All T cells originate from haematopoietic stem cells in the bone marrow. Haematopoietic progenitors (lymphoid progenitor cells ... T helper cells (TH cells) assist other white blood cells in immunologic processes, including maturation of B cells into plasma ... as well as differentiation into mature T cells. The process of positive selection takes a number of days.[34] ... "IL-10 Induces T Cell Exhaustion During Transplantation of Virus Infected Hearts". Cellular Physiology and Biochemistry. 38 (3 ...

*Stem cell marker

"Flk-2 is a marker in hematopoietic stem cell differentiation: A simple method to isolate long-term stem cells". Proceedings of ... June 2005). "Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells". Blood. 105 ... August 2004). "Cellular senescence induced loss of stem cell proportion in the skin in vitro". Journal of Dermatological ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ...

*GDF11

... activates the TGF-β pathway in cardiomyocytes derived from pluripotent hematopoietic stem cells and suppresses the ... "GDF11 modulates NGN3+ islet progenitor cell number and promotes beta-cell differentiation in pancreas development". Development ... Age-reversal effects of 'young blood' molecule GDF-11 called into question, retrieved 20 May 2015 'Young blood' anti-ageing ... "TGFbeta ligands promote the initiation of retinal ganglion cell dendrites in vitro and in vivo". Molecular and Cellular ...

*Optical stretcher

An exemplary study in stem cell research sheds light on the process of cell differentiation: Hematopoietic stem cells residing ... red blood cells and different types of white blood cells. In this study, it was shown that the white blood cell types show ... Cell mechanics and cell rheology play a crucial role in cellular development and also in many diseases. Due to its high ... among them studies on the development of cancer and stem cell differentiation. ...

*White blood cell

... by hematopoietic lineage (cellular differentiation lineage). Lymphocytes can be further classified as T cells, B cells, and ... All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... T cells: CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ...

*Induced stem cells

"The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiation". Blood. 122 (3): 376-85. doi ... although not white blood cells. The glycans on the stem cell surface respond rapidly to alterations in cellular state and ... Human Pluripotent Stem Cell Differentiation and Direct Reprogramming". Cell Stem Cell. 10 (1): 16-28. doi:10.1016/j.stem. ... "Reprogramming of Human Peripheral Blood Cells to Induced Pluripotent Stem Cells". Cell Stem Cell. 7 (1): 20-4. doi:10.1016/j. ...

*T cell

All T cells originate from c-kit+Sca1+ haematopoietic stem cells (HSC) which reside in the bone marrow. In some cases the ... B or NK cells.[2] These CLP cells then migrate via the blood to the thymus, where they engraft. The earliest cells which ... CD4 and CD8 T cells are selected in the thymus, but undergo further differentiation in the periphery to specialized cells which ... "IL-10 Induces T Cell Exhaustion During Transplantation of Virus Infected Hearts". Cellular Physiology and Biochemistry. 38 (3 ...

*MECOM

... has also been shown in vitro to upregulate the cell cycle and block granulocytic differentiation of murine hematopoietic cells ... cellular development and differentiation in the bone marrow leading to dramatic alterations in the normal population of blood ... mouse embryos have been shown to have defects in both the development and proliferation of hematopoietic stem cells (HSCs). It ... Cell cycle and differentiation In vitro experiments using both human and mouse cell lines have shown that EVI1 prevents the ...

*ETV6

... of blood platelet-forming megakaryocytes and blocking the Cellular differentiation of erythroblasts into red blood cells; this ... These translocation mutations commonly occur in pluripotent hematopoietic stem cells that differentiate into various types of ... ETV6 interacts with other proteins that regulate the differentiation and growth of cells. It binds to and thereby inhibits FLI1 ... Sanchez-Martin M, Ferrando A (2017). "The NOTCH1-MYC highway toward T-cell acute lymphoblastic leukemia". Blood. 129 (9): 1124- ...

*MAFB (gene)

"Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells". Cell ... "Balance of MafB and PU.1 specifies alternative macrophage or dendritic cell fate". Blood. 105 (7): 2707-16. doi:10.1182/blood- ... "MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation". Cell. 85 (1): 49-60. doi: ... Molecular and Cellular Biology. 24 (10): 4534-45. doi:10.1128/MCB.24.10.4534-4545.2004. PMC 400447 . PMID 15121870. Petersen HH ...

*Ostra białaczka szpikowa, wolna encyklopedia

DOI: 10.1182/blood-2002-12-3714. PMID: 12714526. *↑ M. Duval, JP. Klein, W. He, JY. Cahn i inni. Hematopoietic stem-cell ... myeloid leukemia does not deplete normal hematopoietic stem cells but induces cytopenias by impeding their differentiation, „ ... Attenuation of AML1-ETO cellular dysregulation correlates with increased leukemogenic potential. „Blood". 121 (18), s. 3714-7, ... preleukemic stem cells: molecular biology and clinical implications of the precursors to leukemia stem cells.. „Stem Cells ...

*Hematopoietic stem cell niche

Blood. 116 (9): 1422-1432. doi:10.1182/blood-2009-08-239194. PMID 20472830. Kohler A; et al. (2009). "Altered cellular dynamics ... "Dynamic niches in the origination and differentiation of haematopoietic stem cells". Nat. Rev. Mol. Cell Biol. 12 (10): 643-655 ... the hematopoietic stem cell (HSC). As these cells are short-lived, there needs to be a steady turnover of new blood cells and ... "Regulation of the HIF-1α level is essential for hematopoietic stem cells". Stem Cell. 7 (3): 391-402. doi:10.1016/j.stem. ...

*Microglia

For a long time it was thought that microglial cells differentiate in the bone marrow from hematopoietic stem cells, the ... "Origin and differentiation of microglia". Frontiers in Cellular Neuroscience. 7: 45. doi:10.3389/fncel.2013.00045. PMID ... During inflammation, T-cells cross the blood-brain barrier thanks to specialized surface markers and then directly bind to ... While moving through its set region, if the microglial cell finds any foreign material, damaged cells, apoptotic cells, ...

*Carboxyfluorescein succinimidyl ester

"High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro ... "Helper T cell differentiation is controlled by the cell cycle". Immunity. 9 (2): 229-37. doi:10.1016/S1074-7613(00)80605-6. ... Gett AV, Hodgkin PD (September 2000). "A cellular calculus for signal integration by T cells". Nature Immunology. 1 (3): 239-44 ... doi:10.1182/blood-2003-06-2070. PMID 14630824.. *^ Hermans IF, Silk JD, Yang J, et al. (February 2004). "The VITAL assay: a ...
Bone marrow transplantation, 2016; doi:10.1038/bmt.2016.123. Authors: Green D J, Bensinger W I, Holmberg L A, Gooley T, Till B G Green D J, Bensinger W I, Holmberg L A, Gooley T, Till B G, Budde L E, Pagel J M, Frayo S L, Roden J E, Hedin L, Press O W, Gopal A K et al.(7) Affiliation: Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; City of Hope National Medical Center, Duarte, CA, United States; Fred Hutchinson Cancer Research Center, United States; Fred Hutchinson Cancer Research Center, United States; ...
OBJECTIVE: To explore the outcome of human leukocyte antigen (HLA)-mismatched/haploidentical hematopoietic stem cell transplantation (HSCT) for refractory/relapsed acute leukemia (AL) patients and its related risk factors.. METHODS: 96 refractory/relapsed AL patients who received HLA-mismatched/haploidentical HSCT following conditioning regimen comprised of modified busulfan/cyclophosphamide (BU/CY) plus thymoglobulin (ATG) from Jan 2003 to Jun 2011 were analyzed retrospectively.. RESULTS: Of the 96 patients, 61 suffered from acute myeloid leukemia (AML), and 35 acute lymphoid leukemia (ALL), all of them in non-remission (NR) or relapse before transplantation. With a median follow-up of 373 (34 - 3157) d, 33 cases (34%) survived, 31 survived without leukemia, and 35 relapsed. The estimated 3-year overall survival (OS) and disease-free survival (DFS) rate was 30.2% and 29.0%, respectively. The 3-year OS rate was significantly higher for AML patients (39.2%) than for ALL ...
According to a recently published report by Brisk Insights, the Global Hematopoietic Stem Cells Transplantation Market is expected to grow at the CAGR of 10.4 % during 2017-2025. The global hematopoietic stem cells transplantation market is segmented on the basis of application, transplant type, and geography. The report on global hematopoietic stem cells transplantation market (by application, transplant type, and geography) provides a detailed overview and predictive analysis of the market.. Full report available on Hematopoietic Stem Cells Transplantation Market: Global Industry Size, Growth, Share and Forecast to 2025 report at ...
Геморрагический энтерит, ассоциированный с парвовирусом В19 после трансплантации гемопоэтических стволовых клеток: клинический случай и данные литературыHaemorrhagic enteritis associated with parvovirus B19 following hematopoietic stem cell transplantation: a case report and literature data
TY - JOUR. T1 - Rapid immune reconstitution following autologous hematopoietic stem cell transplantation in children. T2 - A single institution experience. AU - Hoepfner, S.. AU - Haut, Paul. AU - OGorman, M.. AU - Kletzel, M.. PY - 2003/2. Y1 - 2003/2. N2 - In this retrospective study, we review the immune reconstitution of children undergoing autologous hematopoietic stem cell transplantation. A total of 125 patients underwent autologous transplantation between 1992 and 2000. The report includes data on 58 patients. Data were not available on the remaining patients who either died before testing or data were not obtained. The parameters evaluated include: (a) immunophenotype by flow cytometry to quantify lymphocyte subpopulations (b) mitogen stimulation assays, and (c) quantitative immunoglobulins. The analysis reveals that CD3+ cells did not reach the normal range during the first year post-transplant. ...
A nosocomial pneumonia caused by Legionella pneumophilaserogroup 2oc-14oc curred in a 7-year-old patient following allogeneic hematopoietic stem cell transplantation for thalassemia major. The patient was diagnosed as nosocomial Legionella pneumonia by a polymerase chain reaction (PCR) from bronchoalveolar lavage and a culture of L. pneumophilaserogroup 2-14 from patients room faucet water. Legionella was eradicated from our hospitals water distribution system by superheating and chemical eradication methods (hyper-chlorination and hydrogen peroxide). We did not detect any case after this event. Early recognition of contamination of hospital water system with Leigonella proves the importance of prevention new cases. ...
A 60-year-old man presented with impaired consciousness and psychomotor agitation after a second allogeneic haematopoietic stem cell transplantation (HSCT) from a matched unrelated donor for acute myeloid leukaemia. Clinical, biological and radiological evidence suggested a diagnosis of central nervous system graft-versus-host disease (CNS-GvHD). After intrathecal infusion of methylprednisolone, the clinical symptoms as well as the radiological abnormalities disappeared. The present report illustrates the difficulties in the diagnosis and the management of CNS-GvHD, a very rare and still challenging neurological complication that can occur after allogeneic HSCT. ...
TY - JOUR. T1 - Spinal osteomyelitis due to Aspergillus flavus in a child. T2 - A rare complication after haematopoietic stem cell transplantation. AU - Beluffi, Giampiero. AU - Bernardo, Maria Ester. AU - Meloni, Giulia. AU - Spinazzola, Angelo. AU - Locatelli, Franco. PY - 2008/6. Y1 - 2008/6. N2 - We report the case of a child affected by acute myeloid leukaemia who was treated with allogeneic haematopoietic stem cell transplantation and developed cervicothoracic spinal osteomyelitis due to Aspergillus flavus. The diagnosis was difficult on a clinical basis, but made possible by conventional radiography and MRI.. AB - We report the case of a child affected by acute myeloid leukaemia who was treated with allogeneic haematopoietic stem cell transplantation and developed cervicothoracic spinal osteomyelitis due to Aspergillus flavus. The diagnosis was difficult on a clinical basis, but made possible by conventional radiography and MRI.. KW - Aspergillus. KW ...
Background. Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Marrow mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunosuppressive effects. We launched a multi-center clinical study to examine the safety and feasibility of co-transplantation of MSCs (from third party donors) and haploidentical HSCs into 35 children with severe aplastic anemia (SAA). Methods. A total of 35 children with SAA were enrolled in this multi-center study between January 2014 and December 2016. All patients met the criteria of HLA-mismatched with ⩾5/10 HLA-matched loci. The conditioning regimen for haploidentical hematopoietic stem cell transplantation consisted of busulfan (Bu), cyclophosphamide and ATG. BM and peripheral blood CD34+ ...
With the expression "bone marrow hematopoietic stem cell transplant" we intend a complex procedure used especially, but not only, in the treatment of leukimias and lymphomas. Stem cells can be obtained not only from bone marrow but also from peripheral blood after a specific preconditioning of the patient, or from umbilical-cord blood.. Indications for hematopoietic stem cell transplant are acute leukimias, chronic leukimias, different forms of bone marrow insufficiency, thalassemias, Hodgkin lymphoma, non Hodgkin lymphomas, myelomas, other chronic myeloproliferative diseases, numerous genetic disorders and, as a recent indication, some autoimmune illnesses.. ...
Purpose of reviewCytomegalovirus (CMV) infection is a common opportunistic infection after allogeneic haematopoietic stem cell transplantation (HSCT). CMV surveillance-preemptive therapy is the current preferred approach for preventing CMV disease after HSCT. In contrast, antiviral prophylaxis is no
TY - JOUR. T1 - CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages. AU - Matsuoka, Sahoko. AU - Ebihara, Yasuhiro. AU - Xu, Ming Jiang. AU - Ishii, Takefumi. AU - Sugiyama, Daisuke. AU - Yoshino, Hiroshi. AU - Ueda, Takahiro. AU - Manabe, Atsushi. AU - Tanaka, Ryuhei. AU - Ikeda, Yasuo. AU - Nakahata, Tatsutoshi. AU - Tsuji, Kohichiro. PY - 2001/1/15. Y1 - 2001/1/15. N2 - The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34. Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC. In fetuses and ...
HemoGenyx is a preclinical-stage biotechnology company focused on the discovery, development and commercialisation of novel therapies and treatments for blood diseases, like leukemia and lymphoma. The companys leading technologies aim to change the way in which bone marrow/hematopoietic stem cell (BM/HSC) transplants are performed and improve their efficacy.
TY - JOUR. T1 - Respiratory virus infections in pediatric hematopoietic stem cell transplantation. AU - Luján-Zilbermann, Jorge. AU - Benaim, Ely. AU - Tong, Xin. AU - Srivastava, Deo K.. AU - Patrick, Christian C.. AU - Devincenzo, John. PY - 2001/10/1. Y1 - 2001/10/1. N2 - Respiratory virus infections (RVI) have become an increasingly appreciated problem in the hematopoietic stem cell transplant (HSCT) population. A retrospective analysis of 274 patients undergoing 281 HSCT at St. Jude Childrens Research Hospital from January 1994 through December 1997 was performed. Medical and clinical laboratory records were reviewed beginning at the onset of conditioning through the year following each HSCT, and the analysis was done for the first RVI only. Thirty-two (11%) of 281 HSCT cases developed a RVI during the first year post-HSCT. The most frequent cause of RVI was human parainfluenza virus type 3. Univariate analysis was performed to ...
TY - JOUR. T1 - The effect of pretransplant interferon therapy on the outcome of unrelated donor hematopoietic stem cell transplantation for patients with chronic myelogenous leukemia in first chronic phase. AU - Lee, Stephanie J.. AU - Klein, John P.. AU - Anasetti, Claudio. AU - Antin, Joseph H.. AU - Loberiza, Fausto R.. AU - Bolwell, Brian J.. AU - Lemaistre, Charles F.. AU - Litzow, Mark R. AU - Marks, David. AU - Waller, Edmund K.. AU - Matlack, Marie. AU - Giralt, Sergio. AU - Horowitz, Mary M.. PY - 2001/12/1. Y1 - 2001/12/1. N2 - Various therapeutic options are available for patients with chronic myelogenous leukemia. Allogeneic stem cell transplantation, though often curative, is associated with high nonrelapse mortality and long-term morbidity, particularly when cells from unrelated donors are used. Many physicians and patients opt for a trial of interferon-α (IFN)-based therapy first, reserving transplantation ...
TY - JOUR. T1 - Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. AU - North, Trista E.. AU - De Bruijn, Marella F T R. AU - Stacy, Terryl. AU - Talebian, Laleh. AU - Lind, Evan. AU - Robin, Catherine. AU - Binder, Michael. AU - Dzierzak, Elaine. AU - Speck, Nancy A.. PY - 2002. Y1 - 2002. N2 - Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal ...
TY - JOUR. T1 - Pulmonary graft-versus-host disease after autologous hematopoietic stem cell transplantation. AU - Choi, Sue In. AU - Lee, Eun Joo. AU - Kang, Dong Oh. AU - Lee, Sang Yeub. AU - In, Kwang Ho. AU - Kim, Han Kyeom. AU - Park, Sanghoon. PY - 2016/11/1. Y1 - 2016/11/1. KW - Autologous graft-versus-host disease. KW - Hematopoietic stem cell transplantation. KW - Lung. UR - http://www.scopus.com/inward/record.url?scp=84994388396&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84994388396&partnerID=8YFLogxK. U2 - 10.3904/kjim.2014.110. DO - 10.3904/kjim.2014.110. M3 - Letter. C2 - 26701227. AN - SCOPUS:84994388396. VL - 31. SP - 1181. EP - 1183. JO - Korean Journal of Internal Medicine. JF - Korean Journal of Internal Medicine. SN - 0494-4712. IS - 6. ER - ...
Objectives: Autologous haematopoietic stem cell transplantation (HSCT) with CD34+ cell selection has recently been used in the treatment of refractory Crohns disease, showing good safety and promising efficacy. We investigated the safety and efficacy of HSCT with unselected peripheral blood stem cells (PBSCs) in moderate-severe refractory Crohns disease.. Patients: Four patients (three male, one female; age range 26-45 years) with active moderate-severe Crohns disease (median Crohns Disease Activity Index (CDAI) 319, range 272-345), refractory or intolerant to multiple drugs including infliximab, were enrolled.. Interventions: Unselected PBSCs were collected after mobilisation with cyclophosphamide (CTX) 1.5 g/m2 and granulocyte-colony stimulating factor (G-CSF) 10 μg/kg. The conditioning regimen included CTX 50 mg/kg on days −5 to −2 and rabbit anti-thymocyte globulin (ATG) 2.5 mg/kg on days −4 to −2.. Main outcome ...
TY - JOUR. T1 - The repopulation potential of fetal liver hematopoietic stem cells in mice exceeds that of their adult bone marrow counterparts. AU - Rebel, Vivienne I.. AU - Miller, Cindy L.. AU - Eaves, Connie J.. AU - Lansdorp, Peter M.. PY - 1996/4/15. Y1 - 1996/4/15. N2 - Varying, limiting numbers of unseparated or purified cells (Ly-5.1), either from 14.5-day-old fetal liver (FL) or from adult bone marrow (BM) were coinjected with 105 unseparated BM cells (Ly-5.2) into lethally irradiated adult C57B1/6 recipients (Ly-5.2). The kinetics of donor cell repopulation of the lymphoid and myeloid compartments by Ly-5.1+ donor hematopoietic stem cells (ie, competitive repopulation units [CRU]) were monitored at various time points after the transplantation by Ly-5 analysis of the peripheral white ...
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TY - JOUR. T1 - Thalidomide plus dexamethasone as a maintenance therapy after autologous hematopoietic stem cell transplantation improves progression-free survival in multiple myeloma. AU - Maiolino, Angelo. AU - Hungria, Vania T.M.. AU - Garnica, Marcia. AU - Oliveira-Duarte, Gislaine. AU - Oliveira, Luciana C.O.. AU - Mercante, Daniel R.. AU - Miranda, Eliana C.. AU - Quero, Adriana A.. AU - Peres, Ana L.M.. AU - Barros, José C.. AU - Tanaka, Paola. AU - Magalhães, Roberto P.. AU - Rego, Eduardo M.. AU - Lorand-Metze, Irene. AU - Lima, Carmen S.P.. AU - Renault, Ilana Z.. AU - Braggio, Esteban. AU - Chiattone, Carlos. AU - Nucci, Marcio. AU - de Souza, Carmino A.. PY - 2012/10/1. Y1 - 2012/10/1. N2 - Despite the good response of stem cell transplant (SCT) in the treatment of multiple myeloma (MM), most patients relapse or do not achieve complete remission, suggesting that additional treatment is needed. We assessed the impact of thalidomide in maintenance ...
Leonardo Javier Arcuri published a systematic review comparing patient outcomes when treated with haploidentical vs unrelated donor hematopoietic stem cell transplantation (HSCT)
RATIONALE: Drugs used in chemotherapy, such as carboplatin, thiotepa, and etoposide, work in different ways to stop tumor cells from dividing so they stop growing or die. Combining chemotherapy with autologous stem cell transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells. Isotretinoin may be effective in preventing recurrence of glioma. It is not yet known which regimen of chemotherapy plus autologous stem cell transplantation with or without isotretinoin is more effective in treating recurrent high-grade glioma.. PURPOSE: This randomized phase III trial is studying high-dose chemotherapy or intermediate-dose chemotherapy followed by autologous stem cell transplantation to see how well it works compared to high-dose chemotherapy or intermediate-dose chemotherapy followed by autologous stem cell transplantation and ...
TY - JOUR. T1 - Risk Assessment in Adult T Cell Leukemia/Lymphoma Treated with Allogeneic Hematopoietic Stem Cell Transplantation. AU - ATL Working Group of the Japan Society for Hematopoietic Cell Transplantation. AU - Yoshimitsu, Makoto. AU - Tanosaki, Ryuji. AU - Kato, Koji. AU - Ishida, Takashi. AU - Choi, Ilseung. AU - Takatsuka, Yoshifusa. AU - Fukuda, Takahiro. AU - Eto, Tetsuya. AU - Hidaka, Michihiro. AU - Uchida, Naoyuki. AU - Miyamoto, Toshihiro. AU - Nakashima, Yasuhiro. AU - Moriuchi, Yukiyoshi. AU - Nagafuji, Koji. AU - Miyazaki, Yasuhiko. AU - Ichinohe, Tatsuo. AU - Takanashi, Minoko. AU - Atsuta, Yoshiko. AU - Utsunomiya, Atae. N1 - Copyright © 2017 The American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.. PY - 2017/11/16. Y1 - 2017/11/16. N2 - Disease status at allogeneic hematopoietic cell transplantation (HCT) is an important pretransplant prognostic factor ...
TY - JOUR. T1 - Treatment of thrombotic microangiopathy after hematopoietic stem cell transplantation with recombinant human soluble thrombomodulin. AU - Fujiwara, Hideaki. AU - Maeda, Yoshinobu. AU - Sando, Yasuhisa. AU - Nakamura, Makoto. AU - Tani, Katsuma. AU - Ishikawa, Tatsunori. AU - Nishimori, Hisakazu. AU - Matsuoka, Ken-ichi. AU - Fujii, Nobuharu. AU - Kondo, Eisei. AU - Tanimoto, Mitsune. PY - 2015. Y1 - 2015. N2 - BACKGROUND: Transplant-associated thrombotic microangiopathy (TA-TMA) after hematopoietic stem cell transplantation (HSCT) remains a severe complication associated with underlying endothelial damage. TMA has a high mortality rate with no definite treatments and effective treatments are needed. STUDY DESIGN AND METHODS: The study objective was to retrospectively analyze the outcome of patients receiving recombinant human soluble thrombomodulin (rTM), which has cytoprotective effects against calcineurin inhibitor-induced ...
The Wiskott Aldrich syndrome protein is an essential cytoskeleton regulator found in cells of the hematopoietic lineage and controlling the motility of leukocytes. The impact of WAS gene deficiency on the mobilization of hematopoietic progenitor/stem cells in circulation has remained unexplored but information would be pertinent in the context of autologous gene therapy of the Wiskott Aldrich syndrome disease. The response to granulocyte-colony stimulating factor mobilization was investigated in a murine WAS knock-out model of the disease, by measuring haematological parameters, circulation and engraftment of hematopoietic progenitor/stem cells. In the steady-state, adult WAS knock-out mice have B cell lymphopenia, marked neutrophilia, increased counts of circulating haematopoietic progenitor ...
TY - JOUR. T1 - Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance. AU - Ito, Kyoko. AU - Turcotte, Raphaël. AU - Cui, Jinhua. AU - Zimmerman, Samuel E.. AU - Pinho, Sandra. AU - Mizoguchi, Toshihide. AU - Arai, Fumio. AU - Runnels, Judith M.. AU - Alt, Clemens. AU - Teruya-Feldstein, Julie. AU - Mar, Jessica C.. AU - Singh, Rajat. AU - Suda, Toshio. AU - Lin, Charles P.. AU - Frenette, Paul S.. AU - Ito, Keisuke. PY - 2016/12/2. Y1 - 2016/12/2. N2 - A single hematopoietic stem cell (HSC) is capable of reconstituting hematopoiesis and maintaining homeostasis by balancing self-renewal and cell differentiation. The mechanisms of HSC division balance, however, are not yet defined. Here we demonstrate, by characterizing at the single-cell level a purified and minimally heterogeneous murine Tie2+ HSC population, that these top hierarchical HSCs preferentially undergo symmetric ...
One of the worlds leading bone marrow transplant experts is recommending a significant change to current transplant practice for patients who need marrow or adult stem cells from an unrelated donor to treat hematologic malignancies. Fred Appelbaum, M.D., director of the Clinical Research Division at Fred Hutchinson Cancer Research Center, asserts that bone marrow - not circulating, peripheral blood, which is the current norm - should be the source for unrelated donor adult stem cells for most patients who require a transplant. The reason: because there is less incidence of chronic graft-versus-host disease (GVHD), which can be a debilitating side effect of transplantation.
This case series describes 7 patients with severe chronic graft-versus-host disease (cGvHD) associated with bronchiolitis obliterans (BO) after allogeneic hematopoietic stem cell transplantation (HCT) who underwent lung transplantation. About 20% of patients with severe cGVHD develop BO, which portends a poor prognosis. Lung transplantation may be performed for severe refractory BO. In this group, 6 patients underwent bilateral lung transplantation and 1 underwent single lung transplantation. Explanted lung pathology confirmed BO in all patients. The median survival after lung transplantation in this group was 24 months. The authors conclude that lung transplantation is a possible therapeutic option for certain patients with severe therapy-refractory BO.. ...
TY - JOUR. T1 - Recipient and donor JAK2 46/1 haplotypes are associated with acute graft-versus-host disease following allogeneic hematopoietic stem cell transplantation. AU - Balassa, Katalin. AU - Krahling, Tunde. AU - Remenyi, Peter. AU - Batai, Arpad. AU - Bors, Andras. AU - Kiss, Katalin Piroska. AU - Torbagyi, Eva. AU - Gopcsa, Laszlo. AU - Lengyel, Lilla. AU - Barta, Aniko. AU - Varga, Gergely. AU - Tordai, A.. AU - Masszi, T.. AU - Andrikovics, H.. PY - 2016/7/7. Y1 - 2016/7/7. N2 - Several genetic polymorphisms have been implicated to affect the outcome of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The role of cytokines in acute graft-versus-host disease (aGvHD) is well established and many of the involved cytokines signal through the Janus kinase (JAK) pathways. In this study, we assessed the association of recipient and donor JAK2 46/1 haplotypes and allo-HSCT outcome in a cohort of 124 acute myeloid leukemia ...
TY - JOUR. T1 - Functional association between expression of adhesion molecules and marrow repopulating potential of primitive murine hematopoietic progenitor cells (hpc). AU - Travcpff, C. M.. AU - Voder, M. C.. AU - Hiatt, K.. AU - Srour, Edward. PY - 1997. Y1 - 1997. N2 - A number of adhesion molecules have been implicated in the ability of transplanted hematopoietic stem cells (HSC) to engraft. We investigated whether the expression, or lack thereof, of CD11 a, CD43, CD44, CD49d, CD49e, and CD62L on Sca-1 + lin- cells augments or diminishes the bone marrow (BM) repoputatlng potential of these cells. A total of 103 Sca-l+ lin X+ or Sca-1+ lin X- cells (where X is any of the 6 molecules mentioned above) from B6.Hbbd congenic mice were competitively transplanted along with 3 x 104 C57/BI6 ...
Azole therapy is widely utilized in hematopoietic stem cell transplant (HCT) recipients for the treatment of aspergillus. Complications of voriconazole treatment related to its elevated fluoride content have been described in adults, including reports of symptomatic skeletal fluorosis. We review fluoride levels, clinical, and laboratory data in five pediatric HCT recipients on long-term voriconazole therapy, all found to have elevated serum fluoride levels. Two patients had toxic fluoride levels, one infant had symptoms of significant pain with movement and radiographs confirmed skeletal fluorosis. Monitoring fluoride levels in children, especially with skeletal symptoms, should be considered in patients on long-term voriconazole. ...
Hematopoietic stem/progenitor cells (HSPCs) maintain the hematopoietic system by balancing their self-renewal and differentiation events. Hematopoietic stem cells also migrate to various sites and interact with their specific microenvironment to maintain the integrity of the system. Rho GTPases have been found to control the migration of hematopoietic cells and other cell types. Although the role of RAC1, RAC2 and CDC42 has been studied, the role of RHOA in human hematopoietic stem cells is unclear. By utilizing constitutively active and dominant negative RHOA, we show that RHOA negatively regulates both in vitro and in vivo migration and dominant negative RHOA significantly increased the ...
The impact of ABO incompatibility on hematopoietic stem cell transplantation (HSCT) outcome is controversial. As ABH substances are expressed on tissues and secreted in body fluids, they could drive an immune response in minor ABO-incompatible HSCT. The aim of the study was to investigate the prognostic role of the recipients ABH secretor status.; Patients who underwent minor ABO-incompatible HSCT were included. Secretor status was determined either serologically or by molecular genetics.; Between March 1996 and June 2012, a total of 176 patients received minor ABO-incompatible HSCT and 150 (85%) were secretors. Incidence and severity of acute graft-versus-host disease (GVHD) and chronic GVHD did not differ between secretors and nonsecretors (cumulative incidences ± standard errors: acute GVHD on Day 100, 41 ± 11 and 46 ± 5%, p = 0.59; chronic GVHD at 2 years, 52 ± 13 and 56 ± 5%, p = 0.62, for secretors and nonsecretors, respectively). Additionally, nonrelapse mortality ...
Robust and large-scale expansion of umbilical cord blood stem cells in vitro is necessary for widening the usage of transplantation therapies for the treatment of hematological and immune diseases. The lack of understanding of the complex inter-cellular networks regulating stem cell fate in culture explains the low success met so far for the ex vivo expansion of hematopoietic stem cells. The development of a mathematical model of in vitro hematopoiesis coupled with gene expression profiling led to predictions about the secreted factors that play a crucial role in regulating hematopoietic stem cell self-renewal in culture. We tested 18 putative molecules predicted to display effects on primitive progenitor (Long-Term Culture-Initiating Cell; LTC-IC) output, functionally validating three stimulators (VEGF, ...
Objectives: To carry out a phase I-II trial to elucidate the feasibility and efficacy of high dose cyclophosphamide (CY) supported by autologous peripheral blood stem cell transplantation (PBSCT) in the treatment of severe and refractory autoimmune disease (AD).. Methods: Peripheral blood stem cells (PBSCs) were mobilised during haematological recovery after relatively high dose CY (2 g/m2) for 2 days, followed by administration of granulocyte colony stimulating factor. After collecting PBSCs-more than 2×106 CD34+ cells/kg-by apheresis, CD34+ cells were immunologically selected and cryopreserved. Eight patients were enrolled-five had systemic sclerosis (SSc) alone, one had SSc with systemic lupus erythematosus, one amyopathic dermatomyositis (ADM), and one Wegeners granulomatosis (WG). All of the patients ...
Hematopoietic Stem Cell Biology is the newest installment in the Stem Cell Biology and Regenerative Medicine series, to which it adeptly contributes as it offers a selection of carefully chosen topics so that the readers can understand recent advances in the field of the hematopoietic stem cells and hemato/lymphopoiesis. Each chapter is not a simple review of the topic, but rather an in-depth insight, which allows the reader to attain a multifaceted knowledgebase on hematopoietic stem cells. The chapters, which are authored by the leading experts in the field, cover characteristics of the hematopoietic stem cells at the cellular and molecular levels, offers details in regulatory mechanisms of differentiation mechanisms of ...
Hematopoietic Stem Cell Biology is the newest installment in the Stem Cell Biology and Regenerative Medicine series, to which it adeptly contributes as it offers a selection of carefully chosen topics so that the readers can understand recent advances in the field of the hematopoietic stem cells and hemato/lymphopoiesis. Each chapter is not a simple review of the topic, but rather an in-depth insight, which allows the reader to attain a multifaceted knowledgebase on hematopoietic stem cells. The chapters, which are authored by the leading experts in the field, cover characteristics of the hematopoietic stem cells at the cellular and molecular levels, offers details in regulatory mechanisms of differentiation mechanisms of ...
Quiescent long-term hematopoietic stem cells (LT-HSCs) are efficiently activated by type I interferon (IFN-I). However, this effect remains poorly investigated in the context of IFN-I-inducing virus infections. Here we report that both vesicular stomatitis virus (VSV) and murine cytomegalovirus (MCMV) infection induce LT-HSC activation that substantially differs from the effects triggered upon injection of synthetic IFN-I-inducing agents. In both infections, inflammatory responses had to exceed local thresholds within the bone marrow to confer LT-HSC cell cycle entry, and IFN-I receptor triggering was not critical for this activation. After resolution of acute MCMV infection, LT-HSCs returned to phenotypic quiescence. However, non-acute MCMV infection induced a sustained inflammatory milieu within the bone marrow that was associated with long-lasting impairment of LT-HSC function. In conclusion, our results show that ...
The role of autologous peripheral blood stem cell transplantation (APBSCT) in acute myeloid leukaemia (AML) remains controversial. The current study evaluated the application of APBSCT in a large consecutive series of patients with untreated AML, and compared outcome with a predictive model based on MRC AML10 data. Of 148 evaluable patients, 118 patients entered complete remission (CR) after induction therapy comprising three cycles of daunorubicin, cytosine arabinoside and oral 6-thioguanine. Of these patients, 68 (57%) proceeded to consolidation therapy with two courses of intermediate dose cytosine arabinoside, and stem cell mobilisation, and 40 of these patients (34%) underwent the APBSCT procedure after high dose busulphan conditioning. Harvest quality was the main factor precluding APBSCT. Five-year event-free survival (EFS) in patients who achieved CR was 38% and in APBSCT patients was 57%. There were no transplant-related deaths. No significant ...
TY - JOUR. T1 - An outbreak of human parainfluenza virus 3 infection in an outpatient hematopoietic stem cell transplantation clinic. AU - Sydnor, Emily R M. AU - Greer, Amy. AU - Budd, Alicia P.. AU - Pehar, Miriana. AU - Munshaw, Supriya. AU - Neofytos, Dionissios. AU - Perl, Trish M.. AU - Valsamakis, Alexandra. PY - 2012/9/1. Y1 - 2012/9/1. N2 - Background: Parainfluenza viruses cause respiratory tract infections in adults and children, with peak activity during the spring and summer months. Human parainfluenza virus type 3 (hPIV-3) can contribute to significant morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (HSCT). Methods: Automated surveillance software was used to identify an hPIV-3 outbreak in an HSCT clinic. Active surveillance for respiratory illness and infection control measures were instituted. A retrospective molecular investigation of outbreak viral strains was performed by direct ...
The invasive fungal infection (IFI) is the most common cause of mortality related to autologous stem cell transplant. Taking into account that Saprophytic Aspergillus is usually acquired by inhalation, to protect the bronchial tree just before the tissue invasion is quite attractive. In haematologic patients, as well as those ones subjected to an Allogeneic haematopoietic progenitor cell transplant, there is another group of patients at high risk of Invasive Pulmonary Aspergillosis (IPA). These are those patients with acute myeloid leucemia (AML), submitted to induction, intensification or consolidation polychemotherapy. The IPA incidence rate in these patients, whenever during their evolution, reaches 18-20%, with usual treatments. Furthermore, unlike allogeneic haematopoietic progenitor cell transplant patients, neutropenia was the only IPA risk factor. Nowadays, pharmacologic prophylaxis against IPA, in patients with allogeneic haematopoietic progenitor cell transplant and patients ...
PRIMARY OBJECTIVES:. I. To determine whether mixed or full donor hematopoietic chimerism can be safely established using a non-myeloablative conditioning regimen.. II. To determine whether mixed chimerism can be safely converted to full donor hematopoietic chimerism by infusions of donor lymphocytes (DLI).. III. To evaluate potential efficacy of this approach as a treatment for metastatic renal cancer.. OUTLINE:. CONDITIONING REGIMEN: Patients receive fludarabine phosphate intravenously (IV) on days -4 to -2 and undergo low-dose total-body irradiation (TBI) on day 0.. TRANSPLANTATION: Patients undergo allogeneic peripheral blood stem cell transplant on day 0.. IMMUNOSUPRESSION: Patients receive cyclosporine orally (PO) twice daily (BID) or IV once daily (QD) or BID on days -3 to 35 with taper to day 56, and mycophenolate mofetil PO or IV over 2 hours thrice daily (TID) on days 0-40.. DLI: Patients with stable mixed chimerism on day 56 with ...
TY - JOUR. T1 - A phase I trial of high-dose clofarabine, etoposide, and cyclophosphamide and autologous peripheral blood stem cell transplantation in patients with primary refractory and relapsed and refractory Non-Hodgkin lymphoma. AU - Srivastava, Shivani. AU - Jones, David. AU - Wood, Lisa L.. AU - Schwartz, Jennifer E.. AU - Nelson, Robert P.. AU - Abonour, Rafat. AU - Secrest, Angie. AU - Cox, Elizabeth. AU - Baute, Jay. AU - Sullivan, Cheryl. AU - Kane, Kathleen. AU - Robertson, Michael J.. AU - Farag, Sherif S.. PY - 2011/7/1. Y1 - 2011/7/1. N2 - Clofarabine has significant single-agent activity in patients with indolent and aggressive non-Hodgkin lymphoma and synergizes with DNA-damaging drugs. Treatment, however, may be associated with severe and prolonged myelosuppression. We conducted a phase I trial to determine the maximum tolerated dose (MTD) of clofarabine in combination with high-dose etoposide and cyclophosphamide followed by autologous peripheral ...
Unrelated umbilical cord blood stem cell transplant after failure of haploidentical or matched unrelated donor hematopoietic stem cell transplant.
HIV-positive patients with lymphoma were previously excluded from receiving autologous blood stem cell transplants as treatment, because of concern that these patients compromised immune systems would have a higher risk of infection and poor graft function due to their need for HIV medications. Results from the clinical trial, presented at the American Society of Hematology (ASH) meetings on December 8 at 4:45 p.m. PST, show the concern of additional risk is unfounded. Patients with HIV-associated lymphoma (HAL) who meet standard eligibility criteria should receive an autologous blood stem cell transplant as standard of care or be enrolled in a National Institutes of Health (NIH)-funded clinical trial.. The single-arm, multi-institutional clinical trial-conducted by the Blood and Marrow Transplant Clinical Trials Network (BMT CTN) in collaboration with the AIDS Malignancy Clinical Trials Consortium ...
TY - JOUR. T1 - More immune dysregulation. T2 - Sarcoidosis and chronic graft-versus-host disease after allogeneic stem cell transplant. AU - Manalo, Iviensan F.. AU - Miller, India Ashton. AU - Davis, Loretta S. PY - 2016/3/1. Y1 - 2016/3/1. KW - Allogeneic hematopoietic stem cell transplant. KW - Chronic graft-versus-host disease. KW - Cutaneous manifestations of systemic disease. KW - Cutaneous sarcoidosis. KW - Graft-versus-host-disease. KW - Granulomatous dermatitis. KW - Human leukocyte antigen. KW - Noncaseating granulomas. KW - Sarcoidosis. UR - http://www.scopus.com/inward/record.url?scp=84960876408&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84960876408&partnerID=8YFLogxK. U2 - 10.1016/j.jdcr.2016.01.008. DO - 10.1016/j.jdcr.2016.01.008. M3 - Article. AN - SCOPUS:84960876408. VL - 2. SP - 138. EP - 140. JO - JAAD Case Reports. JF - JAAD Case Reports. SN - 2352-5126. IS - 2. ER - ...
Acronyms and Abbreviations: AGM, aorta-gonad-mesonephros; BFU-E, burst-forming unit-erythroid; BFU-MK, burst-forming unit-megakaryocyte; CAFC, cobblestone area-forming cell; CAR, CXCL12-abundant reticular; CFC, colony-formingcell; CFU-E, colony-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte-macrophage; CFU-MK, colony-forming unit-megakaryocyte; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; EBF, early B-cell factor; ECM, extracellular matrix; EGF, epidermal growth factor; EPO, erythropoietin; EPOR, erythropoietin receptor; FAK, focal adhesion kinase; FL, Flt-3 ligand; G-CSF, granulocyte colony-stimulating factor; G-CSF-R, granulocyte colony-stimulating factor receptor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GM-CSF-R, granulocyte-monocyte colony-stimulating factor receptor; GMP, granulocyte-macrophage progenitor; HSC, hematopoietic stem cell; Ig, immunoglobulin; IL, interleukin; IRF4, interferon regulatory ...
The 40-fold increase in childhood megakaryocyte-erythroid and B-cell leukemia in Down syndrome implicates trisomy 21 (T21) in perturbing fetal hematopoiesis. Here, we show that compared with primary disomic controls, primary T21 fetal liver (FL) hematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors are markedly increased, whereas granulocyte-macrophage progenitors are reduced. Commensurately, HSC and megakaryocyte-erythroid progenitors show higher clonogenicity, with increased megakaryocyte, megakaryocyte-erythroid, and replatable blast colonies. Biased megakaryocyte-erythroid-primed gene expression was detected as early as the HSC compartment. In lymphopoiesis, T21 FL lymphoid-primed multipotential progenitors and early lymphoid progenitor numbers are maintained, but there was a 10-fold reduction in committed PreproB-lymphoid progenitors and the functional B-cell potential of HSC and early lymphoid progenitor is severely ...
TY - JOUR. T1 - Prognostic value of FDG-PET prior to autologous stem cell transplantation for relapsed and refractory diffuse large B-cell lymphoma. AU - Sauter, Craig S.. AU - Matasar, Matthew J.. AU - Meikle, Jessica. AU - Schoder, Heiko. AU - Ulaner, Gary A.. AU - Migliacci, Jocelyn C.. AU - Hilden, Patrick. AU - Devlin, Sean M.. AU - Zelenetz, Andrew D.. AU - Moskowitz, Craig. PY - 2015/4/16. Y1 - 2015/4/16. N2 - High-dose chemotherapy (HDT) plus autologous stem cell transplantation (ASCT) is the standard of care for chemosensitive relapsed and refractory diffuse large B-cell lymphoma (rel/ref DLBCL). Interim restaging with functional imaging by positron emission tomography using 18F-deoxyglucose (FDG-PET) has not been established after salvage chemotherapy (ST) and before HDT-ASCT by modern criteria. Herein, we evaluated 129 patients with rel/ref DLBCL proceeding to HDT-ASCT, with ST response assessment by FDG-PET according to the contemporary Deauville 5-point scale. ...
The regulated lysosomal degradation pathway of autophagy prevents cellular damage and thus protects from malignant transformation. Autophagy is also required for the maturation of various hematopoietic lineages, namely the erythroid and lymphoid ones, yet its role in adult hematopoietic stem cells (HSCs) remained unexplored. While normal HSCs sustain life-long hematopoiesis, malignant transformation of HSCs or early progenitors leads to leukemia. Mechanisms protecting HSCs from cellular damage are therefore essential to prevent hematopoietic malignancies. By conditionally deleting the essential autophagy gene Atg7 in the hematopoietic system, we found that autophagy is required for the maintenance of true HSCs and therefore also of downstream hematopoietic progenitors. Loss of autophagy in HSCs leads to the expansion of a ...
Umbilical cord blood (UCB) is an alternative hematopoietic stem cell (HSC) source that can ameliorate several diseases through transplantation. The purpose of this project is to analyze clinical studies comparing HSCs from a single cord blood unit (CBU) to HSCs from bone marrow, and to explore methods of increasing limited amounts of HSCs. It was found that UCB transplantation in adults is a viable method when a matched bone marrow transplant cannot be identified. Further clinical studies using two CBUs suggest better engraftment and lower risk of relapse. However, double cord blood transplantation has been faced with the challenge of single unit dominance in most studies. Ex vivo expansion of UCB HSCs is another promising method to overcome limited HSC counts.
The global Hematopoietic Stem Cells Transplantation (HSCT) Market is one amongst the enormously classified markets. The global Hematopoietic Stem Cells Transplantation (HSCT) market report offers information related to import and export, along with the current business chain in the market at the global level. It also delivers a plan regarding the expansion of supply and demand of the generated products and offering services compared with the key market players Kite Pharma, Thermo Fisher Scientific, CellGenix Technologie Transfer, Cesca Therapeutics, R&D Systems of the Hematopoietic Stem Cells Transplantation (HSCT) market globally.. Apply here for the sample copy of the report @: https://www.reportsbuzz.com/request-for-sample.html?repid=71502. A deep investigation of the ...
PURPOSE OF REVIEW: Hematopoietic stem cells (HSCs) possess two fundamental characteristics, the capacity for self-renewal and the sustained production of all blood cell lineages. The fine balance between HSC expansion and lineage specification is dynamically regulated by the interplay between external and internal stimuli. This review introduces recent advances in the roles played by the stem cell niche, regulatory transcriptional networks, and metabolic pathways in governing HSC self-renewal, commitment, and lineage differentiation. We will further focus on discoveries made by studying hematopoiesis at single-cell resolution. RECENT FINDINGS: HSCs require the support of an interactive milieu with their physical position within the perivascular niche dynamically regulating HSC behavior. In these microenvironments, transcription factor networks and nutrient-mediated regulation of energy ...
article{a4771b6c-1150-427f-a70a-c2fe50a441ba, abstract = {Diamond-Blackfan anemia (DBA) is a congenital bone marrow failure syndrome characterized by a specific deficiency in erythroid progenitors. Since some patients with DBA develop a reduction in thrombocytes and granulocytes with age, we asked whether multipotent hematopoietic progenitors from DBA patients had normal proliferative capacity in liquid expansion cultures. CD34(+) cells derived from DBA patients showed deficient proliferation in liquid culture containing IL-3, IL-6, and SCF. Single CD34(+) CD38(-) cells from DBA patients exhibited deficient proliferation recruitment in a limiting dilution assay containing IL-3, IL-6, SCF, Tpo, FIL, and G-CSF or containing IL-3, IL-6, and SCF. Our findings suggest that the underlying hematopoietic defect in DBA may not be limited to the erythroid lineage. Since a fraction of DBA patients have a deficiency in ...
Bronchiolitis Obliterans (BO) is an obstructive lung disease that can affect individuals that have undergone a lung or hematopoietic stem cell transplant. BO has been studied most extensively in lung transplant recipients, where it is considered to represent chronic lung rejection. It is the leading cause of death after lung transplant, with mortality rates up to 55%. In hematopoietic stem cell transplantation, BO is thought to be a manifestation of chronic graft-vs-host disease (GVHD). Up to 45% of patients undergoing hematopoietic stem cell transplantation at the NHLBI develop a decline in pulmonary function. Conventional therapy for patients who develop BO consists of augmentation of systemic immunosuppressants. Systemic immunosuppression has limited efficacy for BO and is associated with deleterious consequences including increased risk of infections and decreased ...
The newspaper (Yandu Rong media reporter Wang Jingtian correspondent Li Yiran) I think it is okay to participate in blood donation and donate hematopoietic stem cells. It is good for the community to do more things that are good for the body and mind. On December 27, Mr. Han of an iron and steel plant in a Tangshan iron and steel company successfully donated 183 ml of hematopoietic stem cell suspension in Shijiazhuang, the provincial capital, becoming the 9318th case of the Chinese bone marrow bank, the 440th case of the Hebei branch, and the first of the Tangshan Red Cross Society. 21 non-related hematopoietic stem cell donors. Up to now, nearly 9,000 citizens in Tangshan City have joined the China Bone Marrow Bank and 21 cases have been successfully donated. Simple and kind Master Han, a farmer, is a front-line worker at an ironworks in a steel company in ...
I am Associate Professor of Hematology/ Bone Marrow Transplantation in Medical School of University of Patras/ Greece and Clinical Program Director of the Bone Marrow Transplantation and Leukemia Program at Patras University Hospital. I am German Board Certified in Internal Medicine, Hematology and Oncology. I have been working in the field of medical oncology and hematology for ten years in Germany and for the past seven years in Greece. After completing my medical studies in Greece, I moved (1995-2005) to the Dept. of Hematology/Oncology of Freiburg University / Germany (Dir. Prof. R. Mertelsmann), where I completed my PhD Thesis on hematopoietic stem cell biology and engineering, my internal medicine training, my hematology and oncology training and a specialist training in Hematopoietic Stem Cell Transplantation (HSCT). I have been given the Venia Legendi (Assistant Professorship) of the University of Freiburg in 2004. In 2006, I was ...
Hematopoietic stem cells (HSCs) are the cells that can give rise to all different lineages of differentiated/mature blood cells. HSCs are capable of self-renewal and maintaining the homeostasis of differentiated cells for specific functions. The adult type HSCs were first identified in the para-aortic splanchnopleura/Aorta-Gonad-Mesonephros (PSp/AGM) region, the vitelline and umbilical arteries, yolk sac, and placenta. The current hypothesis is that HSCs differentiate from a "hemogenic endothelium". Core binding factors (CBFs), Runx1 and CBFβ, are essential for the generation of definitive hematopoiesis. We hypothesized that Runx1-CBFβ function is specifically required in the hemogenic endothelium per se for HSC emergence. To test this hypothesis, we selectively disrupted or supplied Runx1-CBFβ function in endothelial ...
CD34+ cells may be isolated from blood samples using immunomagnetic or immunofluorescent methods.. Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes.. Cells observed as CD34+ and CD38- are of an undifferentiated, primitive form; i.e., they are multipotential hemopoietic stem cells. Thus, because of their CD34+ expression, such undifferentiated cells can be sorted out.. In tumors, CD34 is found in alveolar soft part sarcoma, preB-ALL (positive in 75%), AML (40%), AML-M7 (most), ...
TY - JOUR. T1 - Infectious complications following nonmyeloablative allogeneic hematopoietic stem cell transplantation. AU - Busca, Alessandro. AU - Locatelli, F.. AU - Barbui, A.. AU - Ghisetti, V.. AU - Cirillo, D.. AU - Serra, R.. AU - Audisio, E.. AU - Falda, M.. PY - 2003/9. Y1 - 2003/9. N2 - Nonmyeloablative hematopoietic stem cell transplantation (NST) has been explored in hematological malignancies and solid tumors in an attempt to minimize treatment-related toxicity. Whether this approach is associated with reduced risk of infectious complications is unclear. The aim of the current study was to evaluate the infectious complications in a series of 32 consecutive adult patients who received NSTat our institution. Peripheral blood stem cell grafts (n = 30) or marrow grafts (n = 2) were infused from human leukocyte antibody (HLA)·matched sibling (n = 30), partially matched related (n = 1), or unrelated ...
Telomere length analysis of donor-derived bone marrow cells (Fig. 3) and HSCs (Fig. 4) shows substantial telomere shortening during serial HSC transplantation. The difference in mean TRF length (ΔTRF) of bone marrow cells after one round of HSC transplantation is ∼1.5 kb (Fig. 3 B). This agrees reasonably well with the predicted reduction in telomere size assuming that ΔTRF is mainly due to the minimum number of extra population doublings (∼12-13) required for expansion of the fraction of the transplanted HSC population which engraft to the size of the HSC pool in adult mice (∼3-5 × 104 cells; references 3, 4), and that the rate of telomere shortening during division of the transplanted HSCs is 50-100 bp per population doubling, as observed for other mouse cells 14,39,40. However, the extent of telomere shortening during the second round of HSC transplantation (ΔTRF ≈ 5.5 ...
0054] In important embodiments of the invention, the umbilical cord blood cells are fractionated in order to generate enriched cell populations. As used herein, an enriched cell population is a cell population that has been manipulated in order to increase the frequency of a particular cell type in the population relative to the frequency of that cell type prior to manipulation. It is to be understood that the cell type being enriched is one that existed in the population prior to manipulation, and that enrichment results from the removal of other cell types from the population rather than addition of the cell type of interest. Of particular interest according to the invention are cell populations enriched in CD34+, CD133+, or CD34+/CD133+ cells. CD34 and CD133 are cell surface protein (or markers) that have been identified previously as present on hematopoietic progenitor ...
TY - JOUR. T1 - Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. AU - Van Os, R. AU - Van Schie, MLJ. AU - Willemze, R. AU - Fibbe, WE. PY - 2004/7. Y1 - 2004/7. N2 - Previous studies from our laboratory indicate that functional, mature neutrophils are essential for interleukin-8 (IL-8)-induced stem cell mobilization. To study a possible role of neutrophils in granulocyte colony-stimulating factor (G-CSF) induced hematopoietic mobilization, we assessed the number of circulating CD34(+) cells in healthy allogeneic stem cell donors on days 3, 4, and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL-8, Flt3 ligand (FL), ...
Hematopoietic stem cells (HSCs) are essential for the maintenance of the hematopoietic system. However, these cells cannot be maintained or created in vitro, and very little is known about their generation during embryogenesis. Many transcription factors and signaling pathways play essential roles at various stages of HSC development. Members of the ETS (E twenty-six) family of transcription factors are recognized as key regulators within the gene regulatory networks governing hematopoiesis, including the ontogeny of HSCs. Remarkably, although all ETS transcription factors bind the same DNA consensus sequence and overlapping tissue expression is observed, individual ETS transcription factors play unique roles in the development of HSCs. Also, these transcription factors are recurrently used throughout development and their functions are context-dependent, increasing the ...
TY - JOUR. T1 - Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. AU - Hess, David A.. AU - Craft, Timothy P.. AU - Wirthlin, Louisa. AU - Hohm, Sarah. AU - Zhou, Ping. AU - Eades, William C.. AU - Creer, Michael H.. AU - Sands, Mark S.. AU - Nolta, Jan A.. PY - 2008/3/1. Y1 - 2008/3/1. N2 - Transplanted adult progenitor cells distribute to peripheral organs and can promote endogenous cellular repair in damaged tissues. However, development of cell-based regenerative therapies has been hindered by the lack of preclinical models to efficiently assess multiple organ distribution and difficulty defining human cells with regenerative function. After transplantation into β-glucuronidase (GUSB)-deficient NOD/SCID/mucopolysaccharidosis type VII mice, we characterized the distribution of lineage-depleted ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in ...
EV Morozova, YuYu Vlasova, MV Barabanshchikova, NN Mamaev, IM Barkhatov, AL Alyanskii, EI Darskaya, MV Vladovskaya, SN Bondarenko, IS Moiseev, BV Afanasyev RM Gorbacheva Scientific Research Institute of Pediatric Oncology, Hematology and Transplantation; IP Pavlov First Saint Petersburg State Medical University, 6/8 Lva Tolstogo str., Saint Petersburg, Russian Federation, 197022 For correspondence: Elena Vladislavovna Morozova, MD, PhD, 6/8 Lva Tolstogo str., Saint Petersburg, Russian Federation, 197022; e-mail: [email protected] For citation: Morozova EV, Vlasova YuYu, Barabanshchikova MV, et al. Chronic Myeloid Leukemia: Role of Allogeneic Hematopoietic Stem Cell Transplantation in the Era of Tyrosine Kinase Inhibitors. Clinical oncohematology. 2020;13(2):193-8 (In Russ). DOI: 10.21320/2500-2139-2020-13-2-193-198 ABSTRACT Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a radical method of chronic myeloid leukemia (CML) ...
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VA Balashova, VI Rugal, SS Bessmeltsev, SV Gritsaev, NYu Semenova, SV Voloshin, ZhV Chubukina, AV Shmidt, AD Garifullin, IM Zapreeva, AA Kuzyaeva, II Kostroma, AYu Kuvshinov, AV Chechetkin Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024 For correspondence: Valentina Andreevna Balashova, MD, PhD, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024; Tel.: +7(812)717-19-37; e-mail: [email protected] For citation: Balashova VA, Rugal VI, Bessmeltsev SS, et al. Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT. Clinical oncohematology. 2018;11(4):368-77. DOI: 10.21320/2500-2139-2018-11-4-368-377 ABSTRACT Aim. To establish correlation between CD34+ autologous ...
0010] Other researchers have also generated RBCs from ESCs; however, these methods either used non-human/non-primate stem cells or used an embryoid body-dependent method (i.e. no direct differentiation). These methods, however, produced a mixture of erythroid and myeloid cells. See Carotta S, et al., "Directed differentiation and mass cultivation of pure erythroid progenitors from mouse embryonic stem cells," Blood 104:1873-1880 (2004); Chadwick K, et al., "Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells," Blood 102:906-915 (2003); Kaufman D, et al., "Hematopoietic colony-forming cells derived from human embryonic stem ...
Osteoblasts are a key component in the regulation of the hematopoietic stem cell (HSC) niche. Manipulating osteoblast numbers results in a parallel change in HSC numbers. We tested the activity of strontium (Sr), a bone anabolic agent that enhances osteoblast function and inhibits osteoclast activity, on hematopoiesis. In vitro treatment of primary murine osteoblasts with Sr increased their ability to form bone nodules, and in vivo it increased osteoblast number, bone volume, and trabecular thickness and decreased trabecular pattern factor. However, the administration of Sr had no influence on primitive HSCs, although the number of hematopoietic progenitors was higher than in control cells. When Sr-treated mice were used as donors for HSC transplantation, no difference in the engraftment ability was observed, whereas hematopoietic recovery was delayed when they were used as recipients. Despite the changes in ...
Infusion reactions are expected to occur and include nausea, vomiting, fever, rigors or chills, flushing, dyspnea, hypoxemia, chest tightness, hypertension, tachycardia, bradycardia, dysgeusia, hematuria, and mild headache. Premedication with antipyretics, histamine antagonists, and corticosteroids may reduce the incidence and intensity of infusion reactions. Severe reactions, including respiratory distress, severe bronchospasm, severe bradycardia with heart block or other arrhythmias, cardiac arrest, hypotension, hemolysis, elevated liver enzymes, renal compromise, encephalopathy, loss of consciousness, and seizure also may occur. Many of these reactions are related to the amount of DMSO administered. Minimizing the amount of DMSO administered may reduce the risk of such reactions, although idiosyncratic responses may occur even at DMSO doses thought to be tolerated. The actual amount of DMSO depends on the method of preparation of the product for infusion. Limiting the amount of DMSO infused ...
Infusion reactions are expected to occur and include nausea, vomiting, fever, rigors or chills, flushing, dyspnea, hypoxemia, chest tightness, hypertension, tachycardia, bradycardia, dysgeusia, hematuria, and mild headache. Premedication with antipyretic, histamine antagonists, and corticosteroids may reduce the incidence and intensity of infusion reactions.. Severe reactions, including respiratory distress, severe bronchospasm, severe bradycardia with heart block or other arrhythmias, cardiac arrest, hypotension, hemolysis, elevated liver enzymes, renal compromise, encephalopathy, loss of consciousness, and seizure also may occur. Many of these reactions are related to the amount of DMSO administered. Minimizing the amount of DMSO administered may reduce the risk of such reactions, although idiosyncratic responses may occur even at DMSO doses thought to be tolerated. The actual amount of DMSO depends on the method of preparation of the product for infusion. Limiting the amount of DMSO infused ...
TY - JOUR. T1 - Baseline profiles of ocular surface and tear dynamics after allogeneic hematopoietic stem cell transplantation in patients with or without chronic GVHD-related dry eye. AU - Wang, Y.. AU - Ogawa, Y.. AU - Dogru, M.. AU - Tatematsu, Y.. AU - Uchino, M.. AU - Kamoi, M.. AU - Okada, N.. AU - Okamoto, S.. AU - Tsubota, K.. PY - 2010/6/1. Y1 - 2010/6/1. N2 - We evaluated ocular surface alterations in allogeneic hematopoietic stem cell transplantation (HSCT) recipients with or without chronic GVHD-related dry eye in a prospective study. Fifty eyes of 25 post-HSCT patients and 28 eyes of 14 age-matched healthy controls were included. Meibomian gland (MG) obstruction, tear evaporation rate, corneal sensitivity (CS), Schirmer test-I, tear break-up time (BUT) and ocular surface vital staining were examined. Conjunctival impression and brush cytology specimens were collected to evaluate the goblet cell density (GCD) and the inflammatory ...
TY - JOUR. T1 - Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices. AU - Joseph, Matthew. AU - Das, Manjusri. AU - Kanji, Suman. AU - Lu, Jingwei. AU - Aggarwal, Reeva. AU - Chakroborty, Debanjan. AU - Sarkar, Chandrani. AU - Yu, Hongmei. AU - Mao, Hai Quan. AU - Basu, Sujit. AU - Pompili, Vincent J.. AU - Das, Hiranmoy. PY - 2014/10. Y1 - 2014/10. N2 - Despite recent advances in cardiovascular medicine, ischemic diseases remain a major cause of morbidity and mortality. Although stem cell-based therapies for the treatment of ischemic diseases show great promise, limited availability of biologically functional stem cells mired the application of stem cell-based therapies. Previously, we reported a PES-nanofiber based ex vivo stem cell ...
Blood, 2001; 98 (12) doi:. Authors: Zaucha J M, Gooley T, Bensinger W I, Heimfeld S, Chauncey T R Zaucha J M, Gooley T, Bensinger W I, Heimfeld S, Chauncey T R, Zaucha R, Martin P J, Flowers M E, Storek J, Georges G, Storb R, Torok-Storb B et al.(7) Affiliation: Fred Hutchinson Cancer Research Center, United States Abstract: A retrospective analysis of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cell (G-PBMC) products harvested from healthy donors indicates significant variability in both the absolute number and relative proportion of CD34, CD3, and CD14 cells obtained. This report examined whether variations in the cellular composition of G-PBMC products correlated with clinical outcomes after myeloablative allogeneic transplantation. The numbers of CD34, CD3, and CD14 cells infused into 181 human leukocyte antigen (HLA)-identical sibling recipients ...
TY - JOUR. T1 - Human herpesvirus-6B infection in pediatric allogenic hematopoietic stem cell transplant patients. T2 - Risk factors and encephalitis. AU - Miura, Hiroki. AU - Kawamura, Yoshiki. AU - Hattori, Fumihiko. AU - Tanaka, Makito. AU - Kudo, Kazuko. AU - Ihira, Masaru. AU - Yatsuya, Hiroshi. AU - Takahashi, Yoshiyuki. AU - Kojima, Seiji. AU - Sakaguchi, Hirotoshi. AU - Yoshida, Nao. AU - Hama, Asahito. AU - Yoshikawa, Tetsushi. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Background: Human herpesvirus-6B (HHV-6B) infection after allogenic hematopoietic stem cell transplantation (allo-HSCT) is known to be associated with post-transplant limbic encephalitis in adults. Meanwhile, the association between HHV-6B infection and central nervous system complications remains unclear in pediatric allo-HSCT patients. Methods: In this study, HHV-6B infection was monitored for more than 50 days after HSCT using virus isolation and ...
TY - JOUR. T1 - Epidemiology and outcomes of clostridium difficile infections in hematopoietic stem cell transplant recipients. AU - Alonso, Carolyn D.. AU - Treadway, Suzanne B.. AU - Hanna, David B.. AU - Huff, Carol Ann. AU - Neofytos, Dionissios. AU - Carroll, Karen C.. AU - Marr, Kieren A.. PY - 2012/4/15. Y1 - 2012/4/15. N2 - Background. Clostridium difficile is the leading cause of infectious diarrhea among hospitalized patients and is a major concern for patients undergoing hematopoietic stem cell transplantation (HSCT). Risk factors and the natural history of C. difficile infection (CDI) are poorly understood in this population.Methods.We performed a retrospective nested case-control study to describe the epidemiology, timing, and risk factors for CDI among adult patients who received HSCTs at our center from January 2003 through December 2008. Results. The overall 1-year incidence of CDI was 9.2% among HSCTs performed (n=999). The ...
TY - JOUR. T1 - Haematopoietic stem cell mobilization with plerixafor and G-CSF in patients with multiple myeloma transplanted with autologous stem cells. AU - Basak, Grzegorz W.. AU - Jaksic, Ozren. AU - Koristek, Zdenek. AU - Mikala, Gabor. AU - Basic-Kinda, Sandra. AU - Mayer, Jiri. AU - Masszi, Tamas. AU - Giebel, Sebastian. AU - Labar, Boris. AU - Wiktor-Jedrzejczak, Wieslaw. PY - 2011/6/1. Y1 - 2011/6/1. N2 - A proportion of patients with multiple myeloma (MM) who have already undergone autologous stem cell transplantation (autoSCT) might benefit from a further transplantation. For this, they might need to undergo another round of stem cell mobilization. We analyzed retrospectively the outcomes of stem cell mobilization with plerixafor and granulocyte colony-stimulating factor (G-CSF) in a group of 30 patients who had undergone autoSCT previously, and in 46 other patients. The ...
TY - JOUR. T1 - E-selectin and very late activation antigen-4 mediate adhesion of hematopoietic progenitor cells to bone marrow endothelium. AU - Rood, P. M L. AU - Dercksen, M. W.. AU - Cazemier, H.. AU - Kerst, J. M.. AU - Von dem Borne, A. E G K. AU - Gerritsen, W. R.. AU - Van der Schoot, C. E.. PY - 2000. Y1 - 2000. N2 - Adhesion of CD34 + hematopoietic progenitor cells (HPCs) to sinusoidal endothelium probably plays a key role in homing of transplanted CD34+ HPCs to the bone marrow (BM). We have investigated the role of various adhesion molecules in the interaction of purified CD34+ HPCs derived from BM or peripheral blood (PB) and a human BM-derived endothelial cell line. Adhesion of CD34+ HPCs to endothelial cells was measured with the use of a double-color flow microfluorimetric adhesion assay. In this assay, adhesion is measured under stirring ...
Murine acquired immunodeficiency syndrome (MAIDS) induced by defective LP-BM5 murine leukemia virus is a disease with many similarities to human AIDS. Previous studies indicated that the depressed hematopoiesis observed in LP-BM5-infected marrow cultures may be attributable to a defect of hematopoietic stroma. We report here the generation of permanent stromal cell lines from noninfected and LP-BM5-infected marrow cultures. Retrovirus infection was confirmed by polymerase chain reaction for viral genome. The ability of these cell lines to support in vitro hematopoiesis was studied. Results indicated that, when cocultured with normal or infected nonadherent mononuclear cells, noninfected cell lines efficiently supported the production of hematopoietic precursors, whereas viral-infected cell lines induced suppression of both normal and viral-infected progenitors. Expression of cytokine genes in stromal cell lines was also examined. All cell ...
The murine stem cell virus (MSCV) promoter exhibits activity in mouse hematopoietic cells and embryonic stem cells. respectively. The strength of the GFP fluorescence in the body was comparable to the proportion of GFP-positive leukocytes. Moreover, the rate of recurrence of the GFP-expressing leukocytes was significantly correlated with the frequency of GFP-expressing Purkinje cells. These results suggest that the MSCV promoter is useful for preferentially expressing a transgene in Purkinje cells. In addition, the proportion of transduced leukocytes in the peripheral circulation reflects the expression level of the transgene in Purkinje Rabbit Polyclonal to PPP2R3B cells, which can be used as a way to monitor transgene expression properties in the cerebellum without invasive techniques. ...
High-dose chemotherapy and autologous stem cell transplantation involves the administration of high doses of anti-cancer drugs and/or radiation therapy for the purpose of killing cancer cells, followed by the infusion of stem cells to "rescue" or restore bone marrow blood cell production. It is important to understand that high-dose chemotherapy is the treatment for your cancer and damage to the bone marrow is a side effect. Fortunately, the bone marrow can be "rescued" or replaced with stem cells. For autologous stem cell transplantation, stem cells are collected from the patient before high-dose chemotherapy is delivered. The stem cells are collected form the bone marrow or peripheral blood, processed, ...
The term chimerism in the setting of hematopoietic stem cell transplantation refers to the engraftment or presence of lymphohematopoietic cells of donor origin, typically detected by using techniques of DNA analysis (18). Mixed chimerism, in contrast to full donor chimerism, indicates the presence of both donor and recipient lymphohematopoietic cells. Because mixed versus total donor chimerism can now be reliably generated with specific NST regimens and avoided with other NST regimens, investigators must address an important question: is the state of mixed chimerism after transplantation desirable?. There are at least 3 theoretical advantages of mixed chimerism over full chimerism as an approach to transplantation tolerance (19), as follows: 1) mixed chimerism can be achieved with nonmyeloablative regimens that are less toxic, 2) mixed chimeras have better immune function, ...
Introduction. Contents 1. Introduction 2. Summary 3. What are stem cells? 3.1 Adult stem cells 3.2 Core blood stem cells 3.3 Embryonic stem cells 4. Potentially of embryonic stem cells 5. UK Stem Cell bank. 6. Controversial Issues 7. Bibliography 1. Introduction This is a report on stem cells and the stem cell bank The aim of this report to overview stem cell research, including stem cell banks and pitched at general readers of non scientific background. 2 Summary This report consists of brief information on what stem cells are and their sources, the UK Stem ...
These experiments explored mechanisms of control of acute lymphoblastic leukemia following allogeneic hematopoietic stem cell transplantation using a murine model of MHC-matched, minor histocompatibility antigen mismatched transplantation. The central hypothesis examined was that addition of active vaccination against leukemia cells would substantially increase the effectiveness of allogeneic donor lymphocyte infusion against ALL present in the host after transplant. While vaccination did increase the magnitude of type I T cell responses against leukemia cells associated with donor lymphocyte infusion, it did not lead to substantial improvement in long term survival. Analysis of immunological mechanisms of leukemia progression demonstrated that the failure of vaccination was not due to antigen loss in leukemia cells. However, analysis of survival provided surprising findings ...
We recently reported that adult acute myeloid leukemia (AML) patients with granulocytic sarcoma (GS) possessed unique clinical features and poor prognosis. However, the optimal therapeutic strategy for this entity has not been established. Therefore, the aim of this study was to assess the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) for the management of AML with GS. We retrospectively analyzed 503 consecutive adult AML patients (median age, 44 years; range, 15-73 years) who received allo-HSCT. A total of 44 patients (8.7%) had GS before transplantation. Patients with GS achieved comparable survival to those without GS (5-year overall survival (OS), 47% vs 44%, respectively, P=0.621). In patients with GS, excellent outcomes were seen in those that underwent allo-HSCT while in complete remission, whereas nine out of ten patients with GS at the time of transplant experienced a relapse within 6 months after allo-HSCT. Local irradiation for GS ...
Pure Red Cell Aplasia in Major ABO-Mismatched Allogeneic Hematopoietic Stem Cell Transplantation Is Associated with Severe Pancytopenia.
The aorta-gonad-mesonephros (AGM) is a region of embryonic mesoderm that develops during embryonic development from the para-aortic splanchnopleura in chick, mouse and human embryos. It has been suggested that this area, in particular the ventral wall of the dorsal aorta, is one of the primary origins of the definitive haematopoietic stem cell. The aorta-gonad-mesonephros (AGM) region is an area derived from splanchnopleura mesoderm identified in embryonic humans, mice, and non-mammalian vertebrates such as birds and zebrafish. It contains the dorsal aorta, genital ridges and mesonephros and lies between the notochord and the somatic mesoderm, extending from the umbilicus to the anterior limb bud of the embryo. The AGM region plays an important role in embryonic development, being the first autonomous intra-embryonic site for definitive haematopoiesis. Definitive haematopoiesis produces hematopoietic stem cells that have the ...
Pediatric patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) often receive intravenous liposomal amphotericin B (L-AmB) as antifungal prophylaxis. There are no guidelines for antifungal prophylaxis in children in this situation. Caspofungin (CAS), a broad-spectrum echinocandin, could be an effective alternative with lower nephrotoxicity than L-AmB. We retrospectively analyzed the safety, feasibility, and efficacy of CAS in our center, and compared the results with L-AmB as antifungal monoprophylaxis in pediatric patients undergoing HSCT. 60 pediatric patients received L-AmB (1 or 3 mg/kg bw/day) and another 60 patients received CAS (50 mg/m2/day) as antifungal monoprophylaxis starting on day one after HSCT. The median ages of patients receiving L-AmB and CAS were 7.5 years and 9.5 years, respectively. No proven breakthrough fungal infection occurred in either group during the median treatment period of 23 days in the L-AmB group and 24 days in the ...
TY - JOUR. T1 - Long-term follow-up of reduced-intensity allogeneic hematopoietic stem cell transplantation for refractory or relapsed follicular lymphoma. AU - Ono, Yukako. AU - Mori, Takehiko. AU - Kato, Jun. AU - Yamane, Akiko. AU - Shimizu, Takayuki. AU - Kikuchi, Taku. AU - Kohashi, Sumiko. AU - Okamoto, Shinichiro. PY - 2012/9. Y1 - 2012/9. UR - http://www.scopus.com/inward/record.url?scp=84865355006&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84865355006&partnerID=8YFLogxK. U2 - 10.1002/ajh.23261. DO - 10.1002/ajh.23261. M3 - Article. C2 - 22674621. AN - SCOPUS:84865355006. VL - 87. SP - 929. EP - 931. JO - American Journal of Hematology. JF - American Journal of Hematology. SN - 0361-8609. IS - 9. ER - ...
ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION IN THERAPY-RELATED MYELOID NEOPLASMS (t-MN) OF THE ADULT: MONOCENTRIC OBSERVATIONAL STUDY AND REVIEW OF THE LITERATURE.
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. multipotent adult progenitor cells along mesodermal lineages and exhibited the enhanced expression of alkaline phosphatase and production of calcium-containing mineral debris by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells exhibited enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrixConly control groups. The potent combination of angiogenic and osteogenic properties provided by multipotent adult ...
Background: Measures were introduced in both the House and Senate to establish a National Cord Blood Stem Cell Bank Network.. Umbilical cord blood stem cells are obtained from the blood contained in the delivered placenta and umbilical cord, which are normally discarded after childbirth. Obtaining these stem cells presents no inherent moral concerns. Through freezing they can be preserved for many years.. According to findings presented in the House measure, cord blood stem cell transplants can be used for bone marrow reconstitution to treat malignancies such as leukemia and lymphoma, genetic disorders such as sickle cell anemia, and acquired diseases. The findings also claim that cord blood stem cells do not have to be matched as closely as ...
Purpose: : To determine if bone marrow-derived stem cells (BMSC) have the capacity in vitro and in vivo to express retinal pigment epithelial (RPE)-like markers. Methods: : In vitro, mouse Sca-1+ GFP+ cells of bone marrow origin were used in coculture with adult mouse RPE cells. The coculture in a 1:1 ratio was performed with and without cell-cell-contact for up to 3 weeks. Mouse fibroblasts served as a control. Immunocytochemical analysis was performed using monoclonal antibodies (mAbs) against specific RPE markers - cytokeratin, RPE65, MITF - as well as non-RPE markers - opsin (photoreceptors) and glial fibrillary acidic protein (GFAP; glia). In vivo, sodium iodate (NaIO3) was used to damage the RPE. For this study, C57BL/6 mice were injected i.v. with 35 mg/kg NaIO3 followed by the subretinal (s.r.) injection of 3x104 Sca-1+ GFP+ BMSC on day 3. The mice were sacrificed on days 7, 14, 21, ...
Mesenchymal stem cells that come from different cell sources can look similar but behave differently. Bone marrow-derived mesenchymal stem cells tend to be the gold standard for isolating and using mesenchymal stem cells, it is not particularly easy to access these cells from the bone marrow. Because there are other, much more easily accessible mesenchymal stem cells, such as those from the umbilical cord, it is important to establish the differences between the different types of stem cells so that each can be used when most appropriate and when most advantageous.. One important difference is how to isolate the cells and how easy it is to do so. Recent work published this year in ...

Hypomethylating agents are effective in myelodysplastic syndrome | Cancer BiologyHypomethylating agents are effective in myelodysplastic syndrome | Cancer Biology

MDS develops as the consequence of a series of genetic changes in a hematopoietic stem cell. These changes alter normal ... in neoplastic cells may restore normal function to genes that are critical for the control of cellular differentiation and ... resulting in too few healthy blood cells and low blood counts. In its mildest form, MDS may be anemia, low platelets or low ... hematopoietic growth and differentiation, resulting in an accumulation of abnormal, immature myeloid cells in the bone marrow ...
more infohttps://blogs.shu.edu/cancer/2016/09/28/hypomethylating-agents-are-effective-in-myelodysplastic-syndrome/

CD34 cell dose in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cell grafts affects engraftment...CD34 cell dose in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cell grafts affects engraftment...

... genotype on outcome following human leucocyte antigen-identical haematopoietic stem cell transplantation." Clinical and ... "Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells with direct and indirect antigen-presenting ... and CD14 cells obtained. This report examined whether variations in the cellular composition of G-PBMC products correlated with ... CD34 cell dose in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cell grafts affects engraftment ...
more infohttps://scicurve.com/paper/11719357

A HaemAtlas: characterizing gene expression in differentiated human blood cells | Blood JournalA HaemAtlas: characterizing gene expression in differentiated human blood cells | Blood Journal

The differentiation of the hematopoietic stem cell (HSC) into the blood cell lineages, which is depicted as a stepwise process ... The hematopoietic system represents one of the best-studied cellular differentiation processes in mammals. ... Hematopoietic fingerprints: an expression database of stem cells and their progeny. Cell Stem Cell 2007;1:578-591. ... Example of CD19+ B cells isolated from peripheral blood mononuclear cells. (A) Peripheral blood mononuclear cells assessed by ...
more infohttp://www.bloodjournal.org/content/113/19/e1?ijkey=5491901052f0241af41d97030780ad1e2a887fd8&keytype2=tf_ipsecsha&sso-checked=true

https://www.thefreelibrary.com/A+Role+of+the+ABCC4+Gene+Polymorphism+in+Airway+Inflammation+of...-a0547075979https://www.thefreelibrary.com/A+Role+of+the+ABCC4+Gene+Polymorphism+in+Airway+Inflammation+of...-a0547075979

ABCC4 is ubiquitously expressed, with particularly high expression in hematopoietic stem cells and blood cells [15]. Limited ... regulates cAMP cellular levels and controls human leukemia cell proliferation and differentiation," The Journal of Biological ... Periostin is a multifunctional protein expressed in many types of inflammatory cells, such as epithelial cells, mast cells, and ... an ABCC4 polymorphism regulates the cellular levels of cAMP and controls human leukemia cell proliferation and differentiation ...
more infohttps://www.thefreelibrary.com/A+Role+of+the+ABCC4+Gene+Polymorphism+in+Airway+Inflammation+of...-a0547075979

Plus itPlus it

... components of the bone marrow niche and essential for the differentiation of new blood cells from Hematopoietic Stem Cells (HSC ... More specifically, these cells display reduced proliferative and differentiation capacities, an increased cellular senescence, ... Our previous research has shown that mesenchymal stromal cells (MSC), one of the main cellular regulatory ... Molecular and Cellular Biology, Genetics Abstract 2408: Epigenomic characterization of MSC from myeloid malignancies. Manuel ...
more infohttp://cancerres.aacrjournals.org/content/77/13_Supplement/2408

Establishment and regulation of the HSC niche: Roles of osteoblastic and vascular compartments.  - PubMed - NCBIEstablishment and regulation of the HSC niche: Roles of osteoblastic and vascular compartments. - PubMed - NCBI

Hematopoietic stem cells (HSC) are multi-potent cells that function to generate a lifelong supply of all blood cell types. ... Within adult bone marrow, HSC self-renewal and differentiation are thought to be regulated by two major cellular components ... Self-renewal and differentiation properties of adult hematopoietic stem cells (HSC) are tightly regulated within their bone ... Blood cells first emerge in the extraembryonic YS at ~E7.0. The placenta and embryonic AGM region exhibit de novo HSC ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?term=21181885

10. Assessing Human Stem Cell Safety | stemcells.nih.gov10. Assessing Human Stem Cell Safety | stemcells.nih.gov

Human stem cells from virtually every source other than blood-derived hematopoietic stem cells are maintained in tissue culture ... and other chemical substances that promote cellular replication and govern the differentiation of the cultured human stem cells ... Assessing Human Stem Cell Safety. Back to top 10. Assessing Human Stem Cell Safety. The isolation of human stem cells offers ... Transplanting hematopoietic stem cells resident in the bone marrow or isolated from cord blood or circulating peripheral blood ...
more infohttps://stemcells.nih.gov/node/110

Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult BloodPhenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood

... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... bone marrow contains very rare precursor cells with the potential to generate hematopoietic stem cells [3]. Human stem cell ... Hematopoiesis is defined as the orderly sequence of events that replenishes the cellular elements of the blood on a daily basis ...
more infohttps://www.hindawi.com/journals/sci/2011/602483/

In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using...In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using...

... derived from UCB into hepatocyte like cells and also to estimate the effects of combination... ... The aim of the study was to develop a new strategy for the differentiation of hematopoietic stem cell (HSC) ... Mayani H, Lansdorp PM (1998) Biology of human umbilical cord blood-derived hematopoietic stem/progenitor cells. Stem Cells 16: ... Mossman T (1983) Rapid colorimetric assay of cellular growth and survival: a application to proliferation and cytotoxicity ...
more infohttps://link.springer.com/article/10.1007/s10616-011-9337-x

Laura M. Calvi, M.D. -  University of Rochester Medical CenterLaura M. Calvi, M.D. - University of Rochester Medical Center

... hematopoietic stem cells (or HSC), which continuously give rise to all cellular blood components, must strictly regulate their ... "The Chemokine CCL3 Regulates Myeloid Differentiation and Hematopoietic Stem Cell Numbers." Scientific reports.. 2018 Oct 2; 8(1 ... Cellular and Molecular Mechanisms of Microenvironmental Regulation of Hematopoietic Stem Cells in the Bone Marrow To survive ... 1) Molecular mechanisms of osteoblastic regulation of hematopoietic stem cells, 2) Role of osteoblasts in coordinating the ...
more infohttps://www.urmc.rochester.edu/people/20891886-laura-m-calvi/researchers

Laura M. Calvi, M.D. -  University of Rochester Medical CenterLaura M. Calvi, M.D. - University of Rochester Medical Center

... hematopoietic stem cells (or HSC), which continuously give rise to all cellular blood components, must strictly regulate their ... These choices include self-renewal, differentiation, quiescence or death. This essential regulation of stem cells is thought to ... Cellular and Molecular Mechanisms of Microenvironmental Regulation of Hematopoietic Stem Cells in the Bone Marrow To survive ... "The aging hematopoietic stem cell niche: Phenotypic and functional changes and mechanisms that contribute to hematopoietic ...
more infohttps://www.urmc.rochester.edu/people/20891886-laura-m-calvi/patients

Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the...Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the...

This controlled system promotes the efficient differentiation of hematopoietic stem/progenitor cells towards B-cells, NK cells ... cell-enriched hematopoietic cell populations from human UC blood," Cytotherapy, vol. 7, no. 4, pp. 334-344, 2005. View at ... we did not pool cell samples for any of the cellular differentiation and proliferation assays. Of note, these rare primitive ... "Foxo3a is essential for maintenance of the hematopoietic stem cell pool," Cell Stem Cell, vol. 1, no. 1, pp. 101-112, 2007. ...
more infohttps://www.hindawi.com/journals/jir/2013/349067/

Chapter 12: Umbilical cord blood (UCB) progenitor and stem cell biology and therapy - Progenitor and Stem Cell Technologies and...Chapter 12: Umbilical cord blood (UCB) progenitor and stem cell biology and therapy - Progenitor and Stem Cell Technologies and...

Umbilical cord blood (UCB) collected from ... - Selection from Progenitor and Stem Cell Technologies and Therapies [Book] ... progenitor and stem cell biology and therapy M. Shahaduzzaman and A.E. Willing, University of South Florida, USA Abstract: ... self-renewal and differentiation. UCB stem/progenitor cell biology could lead to many exciting cellular therapies for diseases ... recent years a greater interest has grown rapidly in use of UCB as an alternate source of hematopoietic stem/progenitor cells ( ...
more infohttps://www.oreilly.com/library/view/progenitor-and-stem/9781845699840/xhtml/B9781845699840500123.htm

The microenvironment in human myeloid malignancies: emerging concepts and therapeutic implications.  - PubMed - NCBIThe microenvironment in human myeloid malignancies: emerging concepts and therapeutic implications. - PubMed - NCBI

doi: 10.1182/blood-2016-11-696070. Epub 2017 Feb 3. Review; Research Support, Non-U.S. Govt ... This environment provides key regulatory signals for and tightly controls cardinal features of hematopoietic stem cells (HSCs ... including self-renewal, quiescence, differentiation, and migration. These features are essential to maintaining cellular ... Similar to their healthy counterpart, malignant hematopoietic stem cells in myeloid malignancies, such as myeloproliferative ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/28159735

A new model for the human bone marrow niche supports the culture of hematopoietic stem and progenitor cells - Hubrecht InstituteA new model for the human bone marrow niche supports the culture of hematopoietic stem and progenitor cells - Hubrecht Institute

... self-renewal and differentiation is highly regulated by a close contact to cellular components (i.e. vascular endothelial cells ... There, HSCs can self-renew or differentiate into hematopoietic progenitor cells (HPCs) to produce daily all mature blood cells ... A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro. Maaike V.J. Braham, Amélie S.P. ... models of the hematopoietic system aim to support stemness and/or differentiation of hematopoietic stem and progenitor cells ( ...
more infohttps://www.hubrecht.eu/a-new-model-for-the-human-bone-marrow-niche-supports-the-culture-of-hematopoietic-stem-and-progenitor-cells/

Cellular and Molecular Mechanisms Governing Bone Marrow Stem Cells in Gaucher Disease - Full Text View - ClinicalTrials.govCellular and Molecular Mechanisms Governing Bone Marrow Stem Cells in Gaucher Disease - Full Text View - ClinicalTrials.gov

... mesenchymal stem cells (with a special focus on osteogenic differentiation), macrophages and osteoclastic differentiation, B ... Experimental: Bone marrow and blood sampling Other: Bone marrow and blood sampling Bone marrow and blood sampling ... topics the investigators plan will then be focused on characterization and functional analysis of hematopoietic stem cells, ... Cellular and Molecular Mechanisms Governing Bone Marrow Stem Cells, Macrophages and Plasma Cell Biology in Gaucher Disease.. ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01439607?cond=%22gaucher+disease%22&rank=15

Cellular and Molecular Mechanisms Governing Bone Marrow Stem Cells in Gaucher Disease - Full Text View - ClinicalTrials.govCellular and Molecular Mechanisms Governing Bone Marrow Stem Cells in Gaucher Disease - Full Text View - ClinicalTrials.gov

... mesenchymal stem cells (with a special focus on osteogenic differentiation), macrophages and osteoclastic differentiation, B ... Experimental: Bone marrow and blood sampling Other: Bone marrow and blood sampling Bone marrow and blood sampling ... topics the investigators plan will then be focused on characterization and functional analysis of hematopoietic stem cells, ... Cellular and Molecular Mechanisms Governing Bone Marrow Stem Cells, Macrophages and Plasma Cell Biology in Gaucher Disease.. ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01439607?cond=%22Gaucher+disease%22&rank=14

Stem Cell Mysteries Being SolvedStem Cell Mysteries Being Solved

A process that isnt understood well is the ability of embryonic stem cells to differentiate into different types of cells with ... Valproic Acid and Cytokines may Induce Hepatic Differentiation of Embryonic Stem Cells. There is now hope of cellular ... Stem Cells - Cord Blood Stem Cells - Fundamentals Parkinsons Disease Surgical Treatment Genetics and Stem Cells Bone Marrow ... Preferred Term is Hematopoietic stem cell transplantation. In this stem cell from bone marrow are injected into a recipient ...
more infohttps://www.medindia.net/news/stem-cell-mysteries-being-solved-109258-1.htm

Petter Woll group | Department of Medicine, Huddinge | Karolinska InstitutetPetter Woll group | Department of Medicine, Huddinge | Karolinska Institutet

... blood cells of multiple lineages are continuously replenished from a rare population of uncommitted hematopoietic stem cells ( ... However, at what cellular stage and the molecular impact of these mutations promoting leukemic stem cell expansion, as well as ... Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing. Nature immunology 2016 17;6 ... Platelet-biased stem cells reside at the apex of the haematopoietic stem-cell hierarchy. Nature 2013 502;7470 232-6 ...
more infohttps://ki.se/en/medh/petter-woll-group

hematopoietic stem cell | China-Mainland | Sigma-Aldrichhematopoietic stem cell | China-Mainland | Sigma-Aldrich

... all cellular blood components are derived from hematopoietic stem cells.. Stromal Cell Lines For T-Cell Differentiation. T ... Stromal Cell Lines For T-Cell Differentiation. Hematopoietic Stem Cell Media and Cytokines. Hematopoietic Stem Cell ... Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myeloid cells ... Hematopoietic Stem Cell Media and Cytokines. Stemline® II Hematopoietic Stem Cell Media ...
more infohttps://www.sigmaaldrich.com/china-mainland/zh/life-science/stem-cell-biology/hematopoietic-stem-cell.html

FoxOs cooperatively regulate diverse pathways governing neural stem cell homeostasisFoxOs cooperatively regulate diverse pathways governing neural stem cell homeostasis

Foxo3a is essential for maintenance of the hematopoietic stem cell pool. Cell stem cell. 2007;1:101-112. [PubMed] ... p53 and Pten control neural and glioma stem/progenitor cell renewal and differentiation. Nature. 2008;455:1129-1133. [PMC free ... to cellular senescence caused by continuous Wnt signaling contributing to suppression of the number of stem/progenitor cells. ... reserves which produce countless blood cells over a life-time. To control these processes across these various systems, the ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC3285492/?lang=en-ca

Hematopoietic Stem and Progenitor Cells (HSPCs)Hematopoietic Stem and Progenitor Cells (HSPCs)

Blood cells are produced by the proliferation and differentiation of a very small population of pluripotent hema ... Mature blood cells have a finite life-span and must be continuously replaced throughout life. ... but more cells are required to achieve engraftment and the cellular output per transplanted stem cell is lower. This suggests ... To learn more about hematopoietic stem cells and HSPC research, please visit our Hematopoietic Stem and Progenitor Cell ...
more infohttps://www.stemcell.com/hematopoietic-stem-and-progenitor-cells-lp.html

Sequential cellular niches control the generation of enucleated erythrocytes from human pluripotent stem cells | HaematologicaSequential cellular niches control the generation of enucleated erythrocytes from human pluripotent stem cells | Haematologica

... that give rise to multi-lineage hematopoietic cells.8,9 Therefore, we firstly initiated hematopoietic differentiation from hPSC ... Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine. Haematologica. 2010 ... Human pluripotent stem cells (hPSC), including human embryonic stem cells (hESC) and human-induced pluripotent stem cells ( ... Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. ...
more infohttp://www.haematologica.org/node/83927.full.print

Ethical and Safety Issues of Stem Cell-Based TherapyEthical and Safety Issues of Stem Cell-Based Therapy

... factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into ... Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position ... cell surface expression of CD105, CD73, CD90 and the absence of hematopoietic markers CD45, CD34, CD14 or CD11b, CD79α or CD19 ... Keywords: embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, stem cell-based therapy. ...
more infohttp://www.medsci.org/v15p0036.htm

Nucleated red blood cell - WikipediaNucleated red blood cell - Wikipedia

Blood cell lineage Hematopoiesis Erythropoiesis Haematopoiesis Hematopoietic stem cell Hartenstein, V (2006). "Blood cells and ... and the nucleus is ejected as a normal part of cellular differentiation before the cell is released into the bloodstream. Thus ... all vertebrate organisms have hemoglobin-containing cells in their blood and all of these red blood cells contain a nucleus. ... In contrast, a nucleated red blood cell (NRBC), also known by several other names, is a mammalian RBC that contains a cell ...
more infohttps://en.wikipedia.org/wiki/Nucleated_red_blood_cell
  • Younger patients with high-risk disease are considered for front-line chemotherapy approaches followed perhaps by allogeneic stem cell transplantation . (shu.edu)
  • In its mildest form, MDS may be anemia, low platelets or low white blood count, but about 10% to 20% of diagnosed cases progress to acute myeloid leukemia (AML). (shu.edu)
  • For example, the use of monoclonal antibodies (mAbs) and the designation of the cluster of differentiation (CD) markers, of which there are now more than 300, 1 allows hematologists to assign detailed phenotypes to malignant blood cells, which form the basis of decisions on therapeutic intervention. (bloodjournal.org)
  • This approach not only suggests a profound impact of the epigenetic aberrancies on gene expression, but also allowed us to identify TGFβ as the soluble factor that, released by the malignant cells in the bone marrow, appears to trigger the above-mentioned defects in the MDS- and AML-derived MSC. (aacrjournals.org)
  • Thus, the central hypothesis pursued by my laboratory is that osteoblastic cells play a central role in orchestrating microenvironmental control of the behavior of both benign and malignant HSC, and that they can be targeted for therapeutic benefit. (rochester.edu)
  • 5) Therapeutic targeting of the HSC niche to improve HSC expansion (in vivo and in vitro), response to toxic injury and malignant stem cell eradication. (rochester.edu)
  • Acute lymphoblastic leukemia (ALL), characterized by the malignant and uncontrolled proliferation of lymphoid precursor cells within bone marrow (BM), is the hematological disorder with the highest frequency in childhood and the most common cause of mortality in children worldwide [ 1 - 5 ]. (hindawi.com)
  • Similar to their healthy counterpart, malignant hematopoietic stem cells in myeloid malignancies, such as myeloproliferative neoplasms, myelodysplastic syndromes, and acute myeloid leukemia, reside in a highly complex and dynamic cellular microenvironment in the bone marrow. (nih.gov)
  • In 2015, Woll started as an Assistant Professor at the Karolinska Institute where his lab that is located at the Center for Hematology and Regenerative Medicine is focused on unravelling cellular and molecular mechanisms underlying malignant hematopoiesis. (ki.se)
  • Unlimited differentiation potential of iPSCs which can be used in human reproductive cloning, as a risk for generation of genetically engineered human embryos and human-animal chimeras, is major ethical issue, while undesired differentiation and malignant transformation are major safety issues. (medsci.org)
  • Others argue that a good deal more information about the basic biology of human stem cells needs to be accumulated before their therapeutic potential in humans can be assessed. (nih.gov)
  • Individuals interested in conducting research on the physiology and stem cell biology within the bone marrow microenvironment are encouraged to apply. (rochester.edu)
  • In this project, the investigators aimed to study the cellular and molecular mechanisms governing the biology of various cell populations of the bone marrow and blood in these patients in order to define their potential role in the physiopathology of the disease. (clinicaltrials.gov)
  • Recent advances in hematopoietic stem cell biology have resulted in the use of HSC transplants in the treatment of cancers and other immune system disorders. (sigmaaldrich.com)
  • Francesca received her MSc degree in Medical, molecular and cellular biotechnologies from Rome "La Sapienza" university in 2010 and her PhD in Biology applied to human health from "RomaTre" university with a fellowship sponsored by "Istituto Superiore di Sanitá" in 2015. (ki.se)
  • In developmental biology, cellular differentiation is the process where a cell changes from one cell type to another. (wikipedia.org)
  • The lecture series aims to stimulate scientific discussions and to foster a growing network of collaborations in a wide range of biomedical research areas such as genomics, proteomics, cell signaling, human malignancies and chemical biology. (uni-halle.de)
  • Biologic therapies derived from such cells-through tissue regeneration and repair as well as through the targeted delivery of genetic material-are expected to be effective in the treatment of a wide range of medical conditions. (nih.gov)
  • Learning more about this process could prove useful for stem cell-based therapies down the road. (medindia.net)
  • HSPCs are also being investigated in cell-based therapies for non-hematopoietic disorders. (stemcell.com)
  • Additionally, cAMP is involved in the induction and regulation of T helper (Th2) immunity, particularly in allergic asthma via dendritic cells . (thefreelibrary.com)
  • This essential regulation of stem cells is thought to be determined at least in part by the environment, or niche, in which these cells reside. (rochester.edu)
  • The PI3K-AKT-FoxO pathway is integral to lifespan regulation in lower organisms and essential for the stability of long-lived cells in mammals. (pubmedcentralcanada.ca)
  • Mechanistically, integrated transcriptomic, promoter and functional analyses of FoxO deficient NSC cultures identified direct gene targets with known links to the regulation of human brain size and the control of cellular proliferation, differentiation, and oxidative defense. (pubmedcentralcanada.ca)
  • Our hope is that stem cell scientists and clinicians will use the information presented herein as a starting point for more in-depth analysis of ethical and safety issues related to clinical translation of stem cells since controversial regulation and application of stem cell therapy has been falsely celebrated not only in countries with lax medical regulations but also in many developed countries. (medsci.org)
  • Stem cell regulation and hierarchical organization ofhuman skeletal progenitors remain largely unexplored. (stanford.edu)
  • Furthermore, data showing that only cells with immature phenotypes are capable of engraftment and leukemia reconstitution in immunodeficient mice models support this [ 9 - 12 ]. (hindawi.com)
  • This regenerative capacity derives from resident neural stem cells (NSC) which contribute directly to neurogenesis throughout development and adulthood. (pubmedcentralcanada.ca)
  • Moreover, neither B nor NK or dendritic lymphoid-cell populations developed recurrently from highly purified ALL-lymphoid progenitors, and their proliferation and cell cycle status revealed limited proliferative capacity. (hindawi.com)
  • Using chromatin and transcriptional profiling, we show that these stem-cell populations gain activity within the focal adhesion kinase (FAK) signalling pathway, and that inhibiting FAK abolishes new bone formation. (stanford.edu)
  • Pharmacological inhibition of ABCC4 reduces the migration of human dendritic cells, indicating an important role for ABCC4 in human immunology . (thefreelibrary.com)
  • The isolation of human stem cells offers the promise of a remarkable array of novel therapeutics. (nih.gov)
  • Efforts to analyze and assess the safety of using human stem cells in the clinical setting are vitally important to this endeavor. (nih.gov)
  • Transplanted human stem cells are dynamic biological entities that interact intimately with-and are influenced by-the physiology of the recipient. (nih.gov)
  • Before they are transplanted, cultured human stem cells are maintained under conditions that promote either the self-renewing expansion of undifferentiated progenitors or the acquisition of differentiated properties indicative of the phenotype the cells will assume. (nih.gov)
  • After incompletely differentiated human stem cells are transplanted, additional fine-tuning occurs as a consequence of instructions received from the cells' physiologic microenvironments within the recipient. (nih.gov)
  • Assessing human stem cell safety requires the implementation of a comprehensive strategy. (nih.gov)
  • Each step in the human stem cell development process-beginning with identifying and evaluating suitable human stem cell sources-must be carefully scrutinized. (nih.gov)
  • A diversity of opinion exists among researchers about the feasibility of initiating pilot clinical studies using human stem cells. (nih.gov)
  • This hesitancy extends to adult human stem cells of non-hematopoietic origin, even though, by contrast, their plasticity is generally considered to be lower than that of their embryo- and fetus-derived counterparts. (nih.gov)
  • The purpose of pedigree evaluation and/or genetic testing is to establish whether the human stem cells in question are suitable for use in the context of a particular clinical situation. (nih.gov)
  • Danet GH, Luongo JL, Butler G et al (2002) C1qRp defines a new human stem cell population with hematopoietic and hepatic potential. (springer.com)
  • Fiegel HC, Lioznov MV (2003) Cortes-Dericks liver-specific gene expression in cultured human hematopoietic stem cells. (springer.com)
  • Glimm H, Tang P, Clark-Lewis I et al (2002) Ex vivo treatment of proliferating human cord blood stem cells with stroma-derived factor-1 enhances their ability to engraft NOD/SCID mice. (springer.com)
  • Ishikawa F, Drake CJ, Yang S et al (2003) Transplanted human cord blood cells give rise to hepatocytes in engrafted mice. (springer.com)
  • These highly purified reagents are developed from an efficient human cell-based technology, and are excellent choices for your critical inflammation, cancer, stem cell, and antibody development research applications. (sigmaaldrich.com)
  • This review provides an overview of the current status of HSPC research with a focus on (i) assays used to detect and enumerate human and mouse stem and progenitor cells, (ii) phenotypic markers and methods used for their identification and isolation, and (iii) culture systems used to amplify stem and progenitor cells or to promote their differentiation in order to produce large numbers of mature blood cells for transfusion. (stemcell.com)
  • Each of the approximately 100 trillion (1014) cells in an adult human has its own copy or copies of the genome except certain cell types, such as red blood cells, that lack nuclei in their fully differentiated state. (wikipedia.org)
  • Such cells, called somatic cells, make up most of the human body, such as skin and muscle cells. (wikipedia.org)
  • The cells of the inner cell mass go on to form virtually all of the tissues of the human body. (wikipedia.org)
  • Although the cells of the inner cell mass can form virtually every type of cell found in the human body, they cannot form an organism. (wikipedia.org)
  • Identification of the Human Skeletal Stem Cell. (stanford.edu)
  • Here, we report the isolation of a self-renewing and multipotent human skeletal stem cell (hSSC) that generates progenitors of bone, cartilage, and stroma, but not fat. (stanford.edu)
  • In addition, hSSC-derived stroma can maintain human hematopoietic stem cells (hHSCs) in serum-free culture conditions. (stanford.edu)
  • With previous derivation of induced pluripotent stem cells (iPSCs) this problem has been overcome, however current perspectives regarding clinical translation of iPSCs still remain. (medsci.org)
  • Substantial progress on understanding the cell hierarchy within ALL bone marrow (BM) has been recorded in the last few years, suggesting that both primitive cell fractions and committed lymphoid blasts with immature stem cell-like properties contain leukemia-initiating cells. (hindawi.com)
  • The aim of the present study was to investigate the ability of lymphoid progenitors from B-cell precursor ALL BM to proliferate and undergo multilineage differentiation. (hindawi.com)
  • Moreover, the understanding of the mechanisms that damage the earliest steps of the lymphoid developmental program in ALL is incomplete, and the existence of specialized cancer stem cells is still a debate [ 8 ]. (hindawi.com)
  • Lymphoid cells include T cells, B cells, and natural killer cells. (sigmaaldrich.com)
  • In fact, it has recently become evident that osteoblasts can both stimulate and limit HSC expansion, promote quiescence, coordinate HSC mobilization and, when destroyed or mutated, initiate hematopoietic dysfunction. (rochester.edu)
  • Interestingly, a number of quiescence-associated transcription factors were elevated, including the transcriptional repressor Gfi-1, which was highly expressed in primitive CD34 + cells. (hindawi.com)
  • Our data, based on 450k DNA methylation microarrays, show that these abnormalities are shared by the MSC from both malignancies and affect key transcription factors like TBX15, PITX2 or HOXB6, formerly linked to differentiation and development. (aacrjournals.org)
  • It was demonstrated that miR-155 over-expression supports the development and aggresivness of B cell malignancies. (wikipedia.org)
  • Neither the precise origins of the leukemic cell, nor the biological behavior of the hematopoietic primitive cells in the leukemic setting are known. (hindawi.com)
  • The identification of leukemic clones with unrelated DJ rearrangements and the presence of cytogenetic abnormalities on lineage negative cells strongly suggest the participation of primitive cells. (hindawi.com)
  • However, at what cellular stage and the molecular impact of these mutations promoting leukemic stem cell expansion, as well as perturbed hematopoietic differentiation, remains poorly understood. (ki.se)
  • Her PhD project is focused on unravelling at the single cell level the molecular dysregulation underlying leukemic stem cell clonal dominance, clonal evolution and resistance to therapy using a method that combines high-sensitivity mutation detection with whole-transcriptome analysis. (ki.se)
  • After further studies this approach has the potency, for widespread cell replacement therapy for liver diseases. (springer.com)
  • More specifically, these cells display reduced proliferative and differentiation capacities, an increased cellular senescence, or the altered expression of key hematopoietic factors, defects that can provide an explanation for the cytopenia associated with these diseases. (aacrjournals.org)
  • A thorough understanding of how stem cells in the skeleton interpret mechanical stimuli and enact regeneration would shed light on how forces are transduced to the nucleus in regenerative processes. (stanford.edu)
  • Such media have less supportive HSPC capacities over time than the feeder cells, proving the importance of cell-cell contact in such culture systems. (hubrecht.eu)
  • Sequence analysis of small RNA clone libraries comparing miRNA expression to all other organ systems examined established that miR-155-5p was one of five miRNAs (i.e. miR-142, miR-144, miR-150, miR-155, and miR-223) that was specific for hematopoietic cells including B-cells, T-cells, monocytes and granulocytes. (wikipedia.org)