Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION.
Defective nuclei produced during the TELOPHASE of MITOSIS or MEIOSIS by lagging CHROMOSOMES or chromosome fragments derived from spontaneous or experimentally induced chromosomal structural changes.
Tests of chemical substances and physical agents for mutagenic potential. They include microbial, insect, mammalian cell, and whole animal tests.
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.
A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.
Agents that reduce the frequency or rate of spontaneous or induced mutations independently of the mechanism involved.
Agents which affect CELL DIVISION and the MITOTIC SPINDLE APPARATUS resulting in the loss or gain of whole CHROMOSOMES, thereby inducing an ANEUPLOIDY.
A plant family of the order Myrtales, subclass Rosidae, class Magnoliopsida composed of tropical plants with parallel-nerved leaves.
A plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. Bixa contains bixin. Cochlospermum contains arjunolic acid and gum kondagogu (POLYSACCHARIDES).
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
The custard-apple plant family of the order Magnoliales, subclass Magnoliidae, class Magnoliopsida. Some members provide large pulpy fruits and commercial timber. Leaves and wood are often fragrant. Leaves are simple, with smooth margins, and alternately arranged in two rows along the stems.
The smaller, reproductive, transcriptionally inert nucleus in the cells of ciliate protozoans, as distinguished from the larger, vegetative, transcriptionally active MACRONUCLEUS. Micronuclei participate in MEIOSIS and autogamy during GENETIC CONJUGATION.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
An antineoplastic antibiotic produced by Streptomyces caespitosus. It is one of the bi- or tri-functional ALKYLATING AGENTS causing cross-linking of DNA and inhibition of DNA synthesis.
Tests to experimentally measure the tumor-producing/cancer cell-producing potency of an agent by administering the agent (e.g., benzanthracenes) and observing the quantity of tumors or the cell transformation developed over a given period of time. The carcinogenicity value is usually measured as milligrams of agent administered per tumor developed. Though this test differs from the DNA-repair and bacterial microsome MUTAGENICITY TESTS, researchers often attempt to correlate the finding of carcinogenicity values and mutagenicity values.
A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
A vacuum tube equipped with an electron emitting CATHODE and a fluorescent screen which emits visible light when excited by the cathode ray. Cathode ray tubes are used as imaging devises for TELEVISIONS; COMPUTER TERMINALS; TEXT TELECOMMUNICATION DEVICES; oscilloscopes; and other DATA DISPLAY devices.
Input/output devices designed to receive data in an environment associated with the job to be performed, and capable of transmitting entries to, and obtaining output from, the system of which it is a part. (Computer Dictionary, 4th ed.)
The visual display of data in a man-machine system. An example is when data is called from the computer and transmitted to a CATHODE RAY TUBE DISPLAY or LIQUID CRYSTAL display.
Fields representing the joint interplay of electric and magnetic forces.
Materials in intermediate state between solid and liquid.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A trace element that plays a role in glucose metabolism. It has the atomic symbol Cr, atomic number 24, and atomic weight 52. According to the Fourth Annual Report on Carcinogens (NTP85-002,1985), chromium and some of its compounds have been listed as known carcinogens.
Compounds based on imidazolidine dione. Some derivatives are ANTICONVULSANTS.
A group of compounds consisting in part of two rings sharing one atom (usually a carbon) in common.
A purine nucleoside that has guanine linked by its N9 nitrogen to the C1 carbon of ribose. It is a component of ribonucleic acid and its nucleotides play important roles in metabolism. (From Dorland, 28th ed)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A phylum of EUKARYOTES characterized by the presence of cilia at some time during the life cycle. It comprises three classes: KINETOFRAGMINOPHOREA; OLIGOHYMENOPHOREA; and POLYMENOPHOREA.
The chemical reactions involved in the production and utilization of various forms of energy in cells.
The processes by which organisms utilize organic substances as their nutrient sources. Contrasts with AUTOTROPHIC PROCESSES which make use of simple inorganic substances as the nutrient supply source. Heterotrophs can be either chemoheterotrophs (or chemoorganotrophs) which also require organic substances such as glucose for their primary metabolic energy requirements, or photoheterotrophs (or photoorganotrophs) which derive their primary energy requirements from light. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; heterotrophy; chemotrophy; or PHOTOTROPHY) to utilize different sources to meet their nutrients and energy requirements.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
A class of ciliate protozoa. Characteristics include the presence of a well developed oral apparatus and oral cilia being clearly distinct from somatic cilia.
Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
Triazole antifungal agent that is used to treat oropharyngeal CANDIDIASIS and cryptococcal MENINGITIS in AIDS.
Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)
Infection with a fungus of the genus CANDIDA. It is usually a superficial infection of the moist areas of the body and is generally caused by CANDIDA ALBICANS. (Dorland, 27th ed)
A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)
The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.
The formation of micronuclei can only be observed in cells undergoing nuclear division and can be clearly seen using ... Since micronucleus tests must be performed on actively dividing cells, bone marrow stem cells and the erythrocytes they produce ... The cytokinesis-block micronucleus (CBMN) assay was first developed to score micronuclei in cells that completed nuclear ... For instance, many known human carcinogens test positive in mammalian micronucleus tests. In these tests, organisms are treated ...
The micronucleus test in vivo is similar to the in vitro one because it tests for structural and numerical chromosomal ... If this damage is severe enough, it will induce cells to undergo apoptosis. A drawback of treatment is that many genotoxic ... A micronucleus is a small structure separate from the nucleus containing nuclear DNA arisen from DNA fragments or whole ... blood cells. Comet assays are one of the most common tests for genotoxicity. The technique involves lysing cells using ...
The micronucleus test in vivo is similar to the in vitro one because it tests for structural and numerical chromosomal ... If this damage is severe enough, it will induce cells to undergo apoptosis.[12] ... A micronucleus is a small structure separate from the nucleus containing nuclear DNA arisen from DNA fragments or whole ... heritable changes can affect either somatic cells of the organism or germ cells to be passed on to future generations.[1] Cells ...
"A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. ... Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty four tests were ... Those that did survive had increased chromosomal instability in a micronucleus test and numerous skeletal abnormalities by ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ...
The CBMN test is based on the fact that only dividing cells can express micronuclei, which means that only in those cells, ... 2010). "Molecular Mechanisms of Micronucleus, Nucleoplasmic Bridge and Nuclear Bud Formation in Mammalian and Human Cells". ... Another group found that CB inhibits the ability of HeLa cells to undergo cytokinesis by decomposition of the contractile ring ... It appeared that these cells were created through progressive nuclear addition instead of nuclear division. The process by ...
A micronucleus test found a potential increase in DNA damage in mutant mice. A knockout in a human colorectal cancer cell line ... proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest". ... "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ...
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty five tests were ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... a 1.5 fold increase in micronuclei, a reduction in dentate gyrus length and abnormal corneal epithelium and endothelium. A more ... "Centrosomal P4.1-associated protein is a new member of transcriptional coactivators for nuclear factor-kappaB". The Journal of ...
... the micronucleus cell undergoes meiosis. During binary fission, the macronucleus divides amitotically, and the micronucleus ... Another recently experimentally tested difference between micronucleus and macronucleus is the specificity that comes from the ... These include meiosis in micronucleus cells, amitosis in micronucleus cells, and mitosis in micronucleus cells. Micronucleus ... Macronuclei and micronuclei differ in their functions even though they are located within the same cell. The micronucleus is ...
Human embryonic stem cells (hESCs) are able to undergo lineage-specific differentiation into specific types of cells, known as ... "Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression". Molecular Biology of the Cell. 21 (4): 650-663. ... Mitotic cells without functional SON have increased double-stranded DNA breaks and micronuclei formation. Consequently, genome ... However, a formal diagnosis of intellectual disability can only be conducted by a performance of an IQ test score of below 70. ...
Micronucleus test. Normal Heart weight. Normal Brain histopathology. Normal Salmonella infection. Normal[10] ... Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[12][19] Twenty five ... regulation of mitotic nuclear division. • positive regulation of oligodendrocyte differentiation. • regulation of transcription ...
... also tested the effect of citrinin on cell viability, but in an embryonic stem cell line (ESC-B5) in vitro. The ESC-B5 cells ... that this reduction in cell viability was due to apoptosis and not necrosis as CTN exposure led to an increase of nuclear DNA ... radioactivity in the gastrointestinal tract after 30 minutes probably reflected the secreted label by the liver and underwent ... oxidative DNA damage or sister chromatids exchanges but induced micronuclei, aneuploidy and chromosomal aberrations. In vivo it ...
The fourth undergoes mitosis. The two cells exchange a micronucleus. The cells then separate. The micronuclei in each cell fuse ... Cell Res. 166 (2): 475-85. doi:10.1016/0014-4827(86)90492-1. PMID 3743667. Raikov, I.B (1972). "Nuclear phenomena during ... using new sequences of the gene coding for small-subunit rRNA and testing the use of combined data sets to construct ... The cell then divides in two, and each new cell obtains a copy of the micronucleus and the macronucleus. Typically, the cell is ...
Binucleated cells with micronuclei, Inhibition of cell cycle kinetics and proliferation; Sister chromatid induction, ... Nuclear weapons[edit]. Main article: Nuclear weapons design. Depleted uranium can be used as a tamper, or neutron reflector, in ... Epidemiological studies and toxicological tests on laboratory animals point to it as being immunotoxic,[96] teratogenic,[97][98 ... 2004, which found that perhaps a quarter of all UK troops would have been interested in undergoing DU-related monitoring, ...
In micronucleus Test, cells are examined for micronuclei, which are fragments or chromosomes left behind at anaphase, and is ... Mutagens may stimulate unscheduled DNA synthesis that results in more stained nuclear material in cells following exposure to ... Transposon, a section of DNA that undergoes autonomous fragment relocation/multiplication. Its insertion into chromosomal DNA ... Mammalian cell lines such as Chinese hamster V79 cells, Chinese hamster ovary (CHO) cells or mouse lymphoma cells may be used ...
"Catastrophic nuclear envelope collapse in cancer cell micronuclei". Cell. 154 (1): 47-60. doi:10.1016/j.cell.2013.06.007. PMC ... The number of cell divisions undergone by a cell population should be related to the rate of chromosomal change. A chromosomal ... The rates in the cell population being tested should be compared to a reference cell population. This is especially true in low ... Normal cells make errors in chromosome segregation in 1% of cell divisions, whereas cells with CIN make these errors ...
Micronucleus test. Normal Heart weight. Normal Skin Histopathology. Normal Eye Histopathology. Normal ... cell cortex. • brush border. • actomyosin. • myosin complex. • integrin complex. • ruffle. • cell nucleus. • cell leading edge ... Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[76][83] Twenty six ... S100A4 expression is associated with enhanced cell migration through maintenance of cell polarization and inhibition of cell ...
Binucleated cells with micronuclei, Inhibition of cell cycle kinetics and proliferation; Sister chromatid induction, ... Above-ground nuclear tests by the Soviet Union and the United States in the 1950s and early 1960s and by France into the 1970s ... that uses a plutonium-based device to cause a mixture of tritium and deuterium to undergo nuclear fusion. Such bombs are ... Some nuclear fuel comes from nuclear weapons being dismantled,[74] such as from the Megatons to Megawatts Program. ...
The testing is too uncontrolled and unpredictable. Therefore, it has been stated that fMRI has much more testing to do before ... Contrast based on pH depends on changes in the acid/alkaline balance of brain cells when they go active. This too often uses an ... Low persistence of micronucleus frequency in lymphocytes of individuals after cardiac scan". Mutat. Res. Fundam. Mol. Mech. ... nuclear imaging, and X-ray angiography), differences in the mechanism by which this damage takes place suggests that the cancer ...
The testing is too uncontrolled and unpredictable. Therefore, it has been stated that fMRI has much more testing to do before ... Contrast based on pH depends on changes in the acid/alkaline balance of brain cells when they go active. This too often uses an ... Since the early 1990s, fMRI has come to dominate brain mapping research because it does not require people to undergo ... nuclear imaging, and X-ray angiography), differences in the mechanism by which this damage takes place suggests that the cancer ...
... can undergo phenotypic switching and grow as single cells in some environments, and filamentous hyphae in others.[67] ... Parakaryon myojinensis is a unique microorganism larger than a typical prokaryote, but with nuclear material enclosed in a ... Further information: List of microorganisms tested in outer space. A tetrad of Deinococcus radiodurans, a radioresistant ... They evolved from symbiotic bacteria and retain a remnant genome.[59] Like bacteria, plant cells have cell walls, and contain ...
The formation of micronuclei can only be observed in cells undergoing nuclear division and can be clearly seen using ... Since micronucleus tests must be performed on actively dividing cells, bone marrow stem cells and the erythrocytes they produce ... The cytokinesis-block micronucleus (CBMN) assay was first developed to score micronuclei in cells that completed nuclear ... For instance, many known human carcinogens test positive in mammalian micronucleus tests. In these tests, organisms are treated ...
Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. Am J Epidemiol. 1991; (134): 840-850. [ ... DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy. Clin Exp Med. ... Figure 1. Microscopic features of nuclear aspects in nasal epithelial cells. (A) Normal cells; (B) Micronucleus and pyknosis ( ... Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells. Mut Res. ...
In the rat micronucleus test, it was reported that phenacetin induced micronuclei in peripheral blood, but not in bone marrow. ... nuclear area ratio. In addition, we determined cell viability in each chamber using the 3-(4,5-dimethylthiazol-2-yl)-2,5- ... test procedure were defined using a chamber slide that enabled the preparation of fine specimens without undergoing ... In this study, the effects of those foods extracts on the DNA of the Chang liver cells were evaluated using the Single Cell ...
The tests also demonstrated a large number of cells undergoing apoptosis and with nuclear abnormalities related to cell death ... using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have ... Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa ...
The tests also demonstrated a large number of cells undergoing apoptosis and with nuclear abnormalities related to cell death ... using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have ... On the first and last days, the Box and Blocks test, the De Melo Eye-Hand Coordination Test, and transcranial magnetic ... Students t-test with a level of significance of P , 0.05 was employed to verify the existence of standardization in the ...
... appliances through micronuclei test and cytogenetic damage analysis in buccal mucosa epithelial cells of patients undergoing ... Feulgen/Fast Green was used as the staining method, and the number of normal, karyolytic, pyknotic, nuclear buds, bi/ ... t test or ANOVA) and nonparametric tests (Chi-square test, Kruskal-Wallis test, Dunn post-test). The adopted level of ... The sample underwent evaluation for genotoxic effects by means of the micronucleus test (MNT). Bivariate analyses were ...
Histogram represents the fraction of daughter cells with cGAS positive micronuclei or that underwent apoptosis during live-cell ... e, Quantification of aberrant nuclei (nuclear blebs and micronuclei) that are cGAS positive in the transwell migration assay. f ... P-value calculated by log-rank test. e, B16 tumors are injected into opposite flanks of mice and treated as indicated. The ... a, Cell cycle profiles of MCF10A cells were monitored by flow cytometry of Propidium iodide-stained cells treated as indicated ...
Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. Am J Epidemiol 1991;134:840-50. ... However, the same phenomena are also seen in cells undergoing necrosis (5, 32) and cannot be regarded as reliable markers for ... 28). Damage of epithelial cells leads to nuclear abnormalities other than micronuclei (metanucleated cells; ref. 32), such as ... The use of the micronucleus test on exfoliated cells to identify anti-clastogenic action in humans: a biological marker for the ...
Cytogenetic analysis was performed on 2000 cells from each woman and included assessment of micronuclei and nuclear ... rays on epithelial gingival cells during panoramic dental radiography using a differentiated protocol for the micronucleus test ... METHODS: 40 healthy individuals who underwent this procedure for diagnostic purposes on request from their dentists agreed to ... Micronuclei, nuclear projections (broken eggs) and degenerative nuclear alterations (pyknosis, karyolysis, karyorrhexis and ...
... the single-cell alkaline gel electrophoresis (comet) assay, the micronucleus test, and the enumeration of gamma-H2AX nuclear ... It would be unwise to undergo self testing with an online genetic testing service, which then yields a positive BRCA result, ... Additional Testing Needed ?. In order to determine if the BRCA gene mutation is clinically significant, additional testing may ... 16) The-case-against-BRCA-1-and-2-testing.-2(1). Lin, Cheryl, et al. "The case against BRCA 1 and 2 testing." Surgery 149.6 ( ...
The formation of micronuclei can only be observed in cells undergoing nuclear division and can be clearly seen using ... Since micronucleus tests must be performed on actively dividing cells, bone marrow stem cells and the erythrocytes they produce ... The cytokinesis-block micronucleus (CBMN) assay was first developed to score micronuclei in cells that completed nuclear ... For instance, many known human carcinogens test positive in mammalian micronucleus tests. In these tests, organisms are treated ...
Nuclear Envelope Marker (ab16048) has been cited in 456 publications. References for Human, Mouse, Rat, Chk, Xl, Ce, ChHm in ... Genomic Instability Is Induced by Persistent Proliferation of Cells Undergoing Epithelial-to-Mesenchymal Transition. Cell Rep ... Mackenzie KJ et al. cGAS surveillance of micronuclei links genome instability to innate immunity. Nature 548:461-465 (2017). ... Sadaie M et al. Cell-based screen for altered nuclear phenotypes reveals senescence progression in polyploid cells after Aurora ...
The micronucleus test in vivo is similar to the in vitro one because it tests for structural and numerical chromosomal ... If this damage is severe enough, it will induce cells to undergo apoptosis. A drawback of treatment is that many genotoxic ... A micronucleus is a small structure separate from the nucleus containing nuclear DNA arisen from DNA fragments or whole ... blood cells. Comet assays are one of the most common tests for genotoxicity. The technique involves lysing cells using ...
... and as well as CENP-C and CENP-T must undergo active transport via the nuclear pore complexes (Görlich and Kutay, 1999; Stewart ... C) Cells from Fig. 2B plotted depending of the fate of their micronuclei. Chi-squared test: χ2=1.815; d.f.=1; P=0.1779 (n.s., ... 2013). Catastrophic nuclear envelope collapse in cancer cell micronuclei. Cell 154, 47-60. doi:10.1016/j.cell.2013.06.007. ... Cell culture, cell lines and reagents. To construct cell lines stably expressing H2B-Dendra2, HEK293T cells were transfected ...
With a patient, it is necessary to analyze a minimum of 500 metrical figures or cells to see if it had undergone alterations as ... It is not known whether North Korea has tested a smaller nuclear warhead, although it said last year that it had. After one ... The new lab includes an automated microscope and three types of the latest software for testing karyotypes, micronuclei and ... Trump, apparently wanting to emphasize the dangers posed by North Koreas missile tests and nuclear weapons development, used ...
... damage was assessed by examining the frequency of micronuclei in exfoliated buccal cells and the frequency of other nuclear ... that microcomputer workers exposed to VDMs are at risk of significant cytogenetic damage and should periodically undergo ... Keywords : micronucleus test; mutagenicity; electromagnetic fields. · text in English · English ( pdf epdf ) ... CARBONARI, Karina et al. Increased micronucleated cell frequency related to exposure to radiation emitted by computer cathode ...
The micronucleus test in vivo is similar to the in vitro one because it tests for structural and numerical chromosomal ... If this damage is severe enough, it will induce cells to undergo apoptosis.[12] ... A micronucleus is a small structure separate from the nucleus containing nuclear DNA arisen from DNA fragments or whole ... heritable changes can affect either somatic cells of the organism or germ cells to be passed on to future generations.[1] Cells ...
c. Nuclear envelope rupture of micronuclei in G0 phase cells does not result in significant DNA damage. Micronucleation was ... By a multinomial test, the large number of SVs detected in the missegregated chromosome that are unique to each daughter cell ... Because MN3 underwent a 2:1 segregation, the missegregated haplotype was inferred to have a "C" at this polymorphic site. From ... Linking Micronuclei to Chromosome Fragmentation. [Cell. 2015]. *Cell biology: the micronucleus gets its big break. [Nature. ...
... namely increases in micronucleus formation and nuclear areas. Subsequent direct tests involving a subset of prioritized genes ... LBH589 is an oral drug that targets the myelofibrosis cells in the bone marrow and induces cell death by allowing for the ... Genes responsive to rapamycin and serum deprivation are clustered on chromosomes and undergo re-organization within local ... It promotes entry of male GERM CELLS to MEIOSIS, possibly by regulating the translation of mRNAs. DAZ1 occurs within a cluster ...
The two resulting micronucleii in each cell then fuse to produce a diploid micronucleus. The two conjugating cells will then ... Nuclear dimorphism : two functionally distinct kinds of nucleii.. Micronucleus is specialized for sexual exchange (no nucleoli) ... active cells. However, these ideas are not well tested. But lets consider a hypothetical example:. A large ciliate may have ... The surviving (haploid) micronucleii undergo mitotic division, resulting in two identical haploid micronucleii in each ...
The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay ... The addition of Cytochalasin B causes TK6 cells that have undergone more than one nuclear division to be quite large. As a ... Test No. 487: In vitro Mammalian Cell Micronucleus Test. OECD Guidelines for the Testing of Chemicals, Section 4. (2016). ... Aardema, M. J., et al. SFTG international collaborative study on in vitro micronucleus test. III. Using CHO cells. Mutation ...
Light microscope was used to score micronuclei, and independent two-tailed t-test was used for statistical analysis. This was ... The records of 430 patients who underwent cystectomy for transitional cell carcinoma of the bladder between 1981 and 1988 were ... Exploring platelet chemokine antimicrobial activity: nuclear magnetic resonance backbone dynamics of NAP-2 and TC-1. Disruptive ... To address delivery, we tested the hypothesis that ex vivo-cultured bone marrow-derived mesenchymal stem cells (MSCs) can ...
The micronucleus test is a well-established DNA damage assay in human monitoring. The current uses hereby presented and ... Signal transduction in B cells is mediated by B cell receptor (BCR) complexes that are composed of membrane immunoglobulins ( ... Reduction of anabolic signals and alteration of osteoblast nuclear morphology in microgravity. This is the first gene cluster ... NDR1 is a plasma membrane (PM)-localized protein, and undergoes several post-translational modifications including carboxy- ...
Estimation of genomic instability was carried out in blood cultures by test on chromosome aberration and micronuclei test with ... For evaluation of stress expression levels we used the complex of standard psychological tests: questionnaires- for adults and ... fractions of rapidly dividing cells havent contain more MN then bi-nuclear cells. ... MN should be found predominantly in more rapidly dividing cells, which should also undergo more than one mitosis during ...
In an in vivo micronucleus assay, no evidence of chromosomal damage to mouse bone marrow cells was observed following bolus ... The overall response rate in patients whose tumors tested as CTA 3+ was 18% while in those that tested as CTA 2+, it was 6%. ... Patients undergoing a lumpectomy had also completed standard radiotherapy. Patients with ER+ and/or PgR+ disease received ... or histologic and/or nuclear Grade 2 or 3. Patients with a history of CHF, myocardial infarction, Grade 3 or 4 cardiac ...
Micronucleus tests ; Smokeless tobacco ; Chromosome aberrations ; Epidemiologic methods ; Genetic markers ; Epithelial cells ; ... Micronuclei and Other Nuclear Anomalies in Buccal Smears: Methods Development.. Author. Tolbert, P. E. ; Shy, C. M. ; Allen, J ... Exfoliated cells are moribund and undergo degenerative phenomena that can produce extranuclear objects difficult to distinguish ... The exfoliated cell micronucleus assay involves microscope analysis of epithelial smears to determine the prevalence of ...
Moreover, nuclear cefpodoxime tablets brands and prices run-on analysis disclosed that c-jun induction by Ara-C in KG-1 cells ... The possible genotoxic effects of dioxolane and trioxane were studied using the micronucleus test. ... The patient underwent thyroidectomy, and histological examination of the tissue was performed. The roentgen anatomy of the ... These results demonstrate an important role of LRP in smooth muscle cell migration. A pilot study of SPINE test scores and ...
Anti-proliferating cell nuclear antigen (PCNA) antibody was employed for detection of the proliferation rate of enterocytes. ... apoptotic micronuclei). TUNEL-positive cells were counted manually in order to eliminate the nonspecific signals due to the ... the TUNEL method coupled to H&E-stained sections enabled us to univocally identify cells undergoing apoptosis. ... CATC wins vaccine testing licence. 09 Aug 2018. The Center for Aquaculture Technologies Canada (CATC) has received a licence ...
RA treatment caused bleb formation on cell surfaces and lysis of cytoplasmic and nuclear membrane. RA treatment also caused ... Key words: Testicular tumor, cytogenetic test, chromosoma aberration, radiotherapy, chemotherapy.. NEOPLASMA, 47, 1, 2000, 48- ... A third group (7 patients) underwent intraluminal brachytherapy only.. In the first group 17 patients (77%) showed symptomatic ... and the number of micronuclei in binuclear lymphocytes (MN).. In patients treated with ionizing radiation, strong inhibition of ...
... marker test for cancer, traditional medicine for high blood pressure symptoms, chinese medicine in oxford requirements, breast ... two innately trastuzumab resistant HER2 positive breast cancer cell lines. In all cell lines tested, neratinib significantly ... In addition, all cells undergoing combined treatment for 48 h were arrested in metaphase, accompanied by shorter chromosome ... Standardization of counting micronuclei: definition of a protocol to measure genotoxic damage in human exfoliated cells.. ...
decreases in your white blood cell count Your doctor should do a blood test to check your white blood cell count before you ... MMF has been shown to activate the Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway in vitro and in vivo in animals ... Patients with symptoms and signs consistent with any of these infections should undergo prompt diagnostic evaluation and ... DMF was not clastogenic in the in vivo micronucleus assay in the rat. ...

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