Leukemia, Basophilic, Acute: A rare acute myeloid leukemia in which the primary differentiation is to BASOPHILS. It is characterized by an extreme increase of immature basophilic granulated cells in the bone marrow and blood. Mature basophils are usually sparse.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Histamine: An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.Histamine Release: The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Receptors, Histamine H1: A class of histamine receptors discriminated by their pharmacology and mode of action. Most histamine H1 receptors operate through the inositol phosphate/diacylglycerol second messenger system. Among the many responses mediated by these receptors are smooth muscle contraction, increased vascular permeability, hormone release, and cerebral glyconeogenesis. (From Biochem Soc Trans 1992 Feb;20(1):122-5)Receptors, Histamine H2: A class of histamine receptors discriminated by their pharmacology and mode of action. Histamine H2 receptors act via G-proteins to stimulate ADENYLYL CYCLASES. Among the many responses mediated by these receptors are gastric acid secretion, smooth muscle relaxation, inotropic and chronotropic effects on heart muscle, and inhibition of lymphocyte function. (From Biochem Soc Trans 1992 Feb;20(1):122-5)Receptors, Histamine: Cell-surface proteins that bind histamine and trigger intracellular changes influencing the behavior of cells. Histamine receptors are widespread in the central nervous system and in peripheral tissues. Three types have been recognized and designated H1, H2, and H3. They differ in pharmacology, distribution, and mode of action.Histamine Antagonists: Drugs that bind to but do not activate histamine receptors, thereby blocking the actions of histamine or histamine agonists. Classical antihistaminics block the histamine H1 receptors only.Histamine Agonists: Drugs that bind to and activate histamine receptors. Although they have been suggested for a variety of clinical applications histamine agonists have so far been more widely used in research than therapeutically.Histamine H1 Antagonists: Drugs that selectively bind to but do not activate histamine H1 receptors, thereby blocking the actions of endogenous histamine. Included here are the classical antihistaminics that antagonize or prevent the action of histamine mainly in immediate hypersensitivity. They act in the bronchi, capillaries, and some other smooth muscles, and are used to prevent or allay motion sickness, seasonal rhinitis, and allergic dermatitis and to induce somnolence. The effects of blocking central nervous system H1 receptors are not as well understood.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Receptors, Histamine H3: A class of histamine receptors discriminated by their pharmacology and mode of action. Histamine H3 receptors were first recognized as inhibitory autoreceptors on histamine-containing nerve terminals and have since been shown to regulate the release of several neurotransmitters in the central and peripheral nervous systems. (From Biochem Soc Trans 1992 Feb;20(1):122-5)Cell Degranulation: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Histamine H2 Antagonists: Drugs that selectively bind to but do not activate histamine H2 receptors, thereby blocking the actions of histamine. Their clinically most important action is the inhibition of acid secretion in the treatment of gastrointestinal ulcers. Smooth muscle may also be affected. Some drugs in this class have strong effects in the central nervous system, but these actions are not well understood.p-Methoxy-N-methylphenethylamine: A potent mast cell degranulator. It is involved in histamine release.Leukemia, Lymphoid: Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.Leukemia, Experimental: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.Histamine N-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to histamine, forming N-methylhistamine, the major metabolite of histamine in man. EC 2.1.1.8.Tryptases: A family of neutral serine proteases with TRYPSIN-like activity. Tryptases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.Leukemia, Myelogenous, Chronic, BCR-ABL Positive: Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.Receptors, IgE: Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).Pyrilamine: A histamine H1 antagonist. It has mild hypnotic properties and some local anesthetic action and is used for allergies (including skin eruptions) both parenterally and locally. It is a common ingredient of cold remedies.Chymases: A family of neutral serine proteases with CHYMOTRYPSIN-like activity. Chymases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.Immunoglobulin E: An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Histidine Decarboxylase: An enzyme that catalyzes the decarboxylation of histidine to histamine and carbon dioxide. It requires pyridoxal phosphate in animal tissues, but not in microorganisms. EC 4.1.1.22.Methylhistamines: Histamine substituted in any position with one or more methyl groups. Many of these are agonists for the H1, H2, or both histamine receptors.Basophils: Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Anaphylaxis: An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.Dimaprit: A histamine H2 receptor agonist that is often used to study the activity of histamine and its receptors.Precursor Cell Lymphoblastic Leukemia-Lymphoma: A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.Cromolyn Sodium: A chromone complex that acts by inhibiting the release of chemical mediators from sensitized mast cells. It is used in the prophylactic treatment of both allergic and exercise-induced asthma, but does not affect an established asthmatic attack.Mastocytosis: A heterogenous group of disorders characterized by the abnormal increase of MAST CELLS in only the skin (MASTOCYTOSIS, CUTANEOUS), in extracutaneous tissues involving multiple organs (MASTOCYTOSIS, SYSTEMIC), or in solid tumors (MASTOCYTOMA).Histamine H3 Antagonists: Drugs that selectively bind to but do not activate HISTAMINE H3 RECEPTORS. They have been used to correct SLEEP WAKE DISORDERS and MEMORY DISORDERS.Peer Group: Group composed of associates of same species, approximately the same age, and usually of similar rank or social status.Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Videotape Recording: Recording of visual and sometimes sound signals on magnetic tape.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Peer Review: An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.Thymus Gland: A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Stromal Cells: Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.Mediastinum: A membrane in the midline of the THORAX of mammals. It separates the lungs between the STERNUM in front and the VERTEBRAL COLUMN behind. It also surrounds the HEART, TRACHEA, ESOPHAGUS, THYMUS, and LYMPH NODES.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Mediastinal Neoplasms: Tumors or cancer of the MEDIASTINUM.Lymphoid Tissue: Specialized tissues that are components of the lymphatic system. They provide fixed locations within the body where a variety of LYMPHOCYTES can form, mature and multiply. The lymphoid tissues are connected by a network of LYMPHATIC VESSELS.

*Mast cell

... including phospholipase Cγ1 and the receptor βγ2 complex, in RBL-2H3 rat basophilic leukemia cells". Molecular and Cellular ... and allergic rhinitis and allergic conjunctivitis. Antihistamine drugs act by blocking histamine action on nerve endings. ... The activities of the latter are dependent on T-cells. Mast cells are present in most tissues characteristically surrounding ... Other neoplastic disorders associated with mast cells include mast cell sarcoma and mast cell leukemia. Mast cell activation ...

*Thymus

The ability of T cells to recognize foreign antigens is mediated by the T-cell receptor. The T-cell receptor undergoes genetic ... Allergies involve mainly IgE, antibodies, and histamine. Mast cells release the histamine. Sometimes an allergen may cause a ... Patients may present with a profound immunodeficiency disease, due to the lack of T cells. No other immune cell lineages are ... Lymphomas or leukemias of thymocyte origin are classified as Precursor T acute lymphoblastic leukemia/lymphoma (T-ALL). People ...

*White blood cell

T cells: CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Histiocytes Dendritic cells (Although these will often migrate to local lymph nodes upon ingesting antigens) Mast cells ... Neutrophils are active in phagocytosing bacteria and are present in large amount in the pus of wounds. These cells are not able ...

*Eosinophil

Zheutlin L, Ackerman S, Gleich G, Thomas L (1984). "Stimulation of basophil and rat mast cell histamine release by eosinophil ... Eosinophils, along with basophils and mast cells, are important mediators of allergic responses and asthma pathogenesis and are ... PDGF-BB in hypereosinophilic leukemia Monoclonal antibodies such as dupilumab and lebrikizumab target IL-13 and its receptor, ... which reduces eosinophilic inflammation in pateints with asthma due to lowering the number of adhesion molecules present for ...

*Innate immune system

These cells present receptors contained on the surface or within the cell, named pattern recognition receptors (PRRs), which ... When activated, mast cells rapidly release characteristic granules, rich in histamine and heparin, along with various hormonal ... Leukemia. 8: 652-58. Klingemann H-G. Development and testing of NK cell lines. In Lotze MT & Thompson AW (eds): Natural killer ... histamine-releasing basophils are important in the defense against parasites and play a role in allergic reactions, such as ...

*Antibody

For example, IgE is responsible for an allergic response consisting of mast cell degranulation and histamine release. IgE's Fab ... It is part of the B cell receptor (BCR), which allows a B cell to detect when a specific antigen is present in the body and ... Williams, CM; Galli, SJ (May 2000). "The diverse potential effector and immunoregulatory roles of mast cells in allergic ... expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia". Blood. ...

*Inflammation

... dendritic cells, histiocytes, Kupffer cells and mast cells. These cells possess surface receptors known as pattern recognition ... "Mast cell proteases as pharmacological targets". European Journal of Pharmacology. Pharmacological modulation of Mast cells and ... Pre-sensitised mast cells respond by degranulating, releasing vasoactive chemicals such as histamine. These chemicals propagate ... leads to a progressive shift in the type of cells present at the site of inflammation, such as mononuclear cells, and is ...

*White blood cell

T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Neutrophils are active in phagocytosing bacteria and are present in large amount in the pus of wounds. These cells are not able ... Blood cell dysfunction - megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement, acute leukemia ...

*White blood cell

T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Neutrophils are active in phagocytosing bacteria and are present in large amount in the pus of wounds. These cells are not able ... Blood cell dysfunction - megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement, acute leukemia ...

*White blood cell

T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Neutrophils are active in phagocytosing bacteria and are present in large amount in the pus of wounds. These cells are not able ... Blood cell dysfunction - megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement, acute leukemia ...

*White blood cell

T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Neutrophils are active in phagocytosing bacteria and are present in large amount in the pus of wounds. These cells are not able ... Blood cell dysfunction - megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement, acute leukemia ...

*Antibody

IgE is responsible for an allergic response consisting of mast cell degranulation and histamine release. IgE's Fab paratope ... It is part of the B cell receptor (BCR), which allows a B cell to detect when a specific antigen is present in the body and ... Binds to allergens and triggers histamine release from mast cells and basophils, and is involved in allergy. Also protects ... expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia". Blood. ...

*Leukocyte

Mast cell precursors. Dendritic cells (Langerhans cells, Follicular dendritic cells). Monocytes/Macrophages (Histiocytes, ... dang papil deng basophil ya pin ing reaksiyon a allergic ampong antigen kapamilatan ning pamagpalual king kemikal a histamine, ... Deng γδ T cell atin lang alternatibung T cell receptor, e anti kareng CD4+ and CD8+ αβ T cell, at kayawig do reng helper T cell ... present) lang kapirasu da reng mikrobius kareng T cell, ba rong akilalang pasibayu at paten deti, o ba yang makapanablas ing ...
We have studied the cytotoxic activity of rat basophilic leukemia (RBL) cells transfected with cDNAs for the cytotoxic T lymphocyte (CTL) granule components, cytolysin (perforin), granzyme A, and granzyme B. With red cell targets, cytolysin expression conferred potent hemolytic activity, which was not influenced by coexpression of granzymes. With tumor targets, RBL cells expressing cytolysin alone were weakly cytotoxic, but both cytolytic and nucleolytic activity were enhanced by coexpression of granzyme B. RBL cells expressing all three CTL granule components showed still higher cytotoxic activities, with apoptotic target death. Analysis of the cytotoxic activity of individual transfectant clones showed that cytolytic and nucleolytic activity correlated with granzyme expression but was independent of cytolysin expression within the range examined. A synergism between ...
By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results ...
TY - JOUR. T1 - Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons. AU - Laeng, H.. AU - Harris, David T.. AU - Schindler, R.. PY - 1985. Y1 - 1985. N2 - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, ...
TY - JOUR. T1 - A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells. AU - Barth, Erin E.. AU - Hallworth, Richard J.. AU - Nichols, Michael G.. PY - 2005/5. Y1 - 2005/5. N2 - Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint will necessarily depend on the characteristics of the damage induced by the agent. By using multiple biological probes, it is possible to get a more complete description of the type of damage induced. Photodamage was induced in rat basophilic leukemia cells by either 254-nm UVC light exposure or rose bengal photosensitization. Damage was measured by three quantitative assays employing fluorescent probes: calcein, to measure nonspecific ...
Background: Mast cells infiltrate the bronchial smooth muscle (BSM) in asthmatic patients, but the mechanism of mast cell adhesion is still unknown. The adhesion molecules CD44 (i.e. hyaluronate receptor) and CD51 (i.e. vitronectin receptor) are widely expressed and bind to many extracellular matrix (ECM) proteins. The aims of the study are (i) to identify the role of ECM in mast cell adhesion to BSM and (ii) to examine the role of CD51 and CD44 in this adhesion.. Methods: Human lung mast cells, human mast cell line (HMC-1), and BSM cells from control donors or asthmatic patients were cultured in the presence/absence of various cytokines. Mast cell-BSM interaction was assessed using 3H-thymidine-pulsed mast ...
TY - JOUR. T1 - Mechanism of bradykinin-induced histamine release from rat peritoneal mast cells. AU - Zhao, Qiu E.. AU - Mihara, Takuma. AU - Sugimoto, Yukio. AU - Kamei, Chiaki. PY - 1996/2. Y1 - 1996/2. N2 - Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same ...
Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat basophilic leukaemia cell line RBL-2H3, and compared their properties with each other and with plasma-membrane vesicles. DRM fractions were isolated as sealed unilamellar vesicles of similar size (135-170 nm diameter), using either sucrose-density-gradient sedimentation or gel-filtration chromatography. Lipid rafts isolated using Brij-96 and Triton X-100 ...
Interactions between products of the mouse W locus, which encodes the c-kit tyrosine kinase receptor, and the Sl locus, which encodes a ligand for c-kit receptor, which we have designated stem cell factor (SCF), have a critical role in the development of mast cells. Mice homozygous for mutations at either locus exhibit several phenotypic abnormalities including a virtual absence of mast cells. Moreover, the c-kit ligand SCF can induce the proliferation and maturation of normal mast cells in vitro or in vivo, and also can result in repair of the mast cell deficiency of Sl/Sld mice in vivo. We now report that administration of SCF intradermally in vivo results in dermal mast cell activation and a mast cell-dependent acute inflammatory ...
c-kit ligand (KL) activated mouse bone marrow-derived mast cells (BMMC) for the dose- and time-dependent release of arachidonic acid from cell membrane phospholipids, with generation of leukotriene (LT) C4 in preference to prostaglandin (PG)D2. KL at concentrations of 10 ng/ml elicited half-maximal eicosanoid generation and at concentrations of , 50 ng/ml elicited a maximal generation of approximately 15 ng LTC4 and 1 ng PGD2 per 10(6) cells, with 20% net beta-hexosaminidase release 10 min after stimulation. Of the other cytokines tested, none, either alone or in combination with KL, elicited or modulated the immediate phase of mediator release by BMMC, indicating strict specificity for KL. Activation of BMMC in response to KL was accompanied by transient phosphorylation of cytosolic phospholipase A2 and reversible translocation of 5-lipoxygenase to a cell membrane fraction 2-5 min after ...
Resveratrol, a polyphenol abundant in peanuts, red wine and the skin of grapes, has been shown to have anti-cancer, anti-oxidant and anti-inflammatory activities, and may also have beneficial effects on allergic inflammation. We investigated the effects of resveratrol on human mast cell activation in comparison to the anti-allergy drug tranilast. In LAD2 mast cells, both resveratrol and tranilast inhibited degranulation induced by the mast cell activators substance P, IgE/anti-IgE, and compound 48/80. Resveratrol inhibition was immediate, preventing degranulation when added simultaneously to physiological stimuli, and the effect was sustained for up to 24 hrs. The inhibitory effect was not cAMP dependent, but may be attributable to calcium modulation, as resveratrol, and to a lesser extent tranilast, prevented substance P-induced increases in intracellular ...
TY - JOUR. T1 - Leflunomide inhibits PDK1/Akt pathway and induces apoptosis of human mast cells. AU - Sawamukai, Norifumi. AU - Saito, Kazuyoshi. AU - Yamaoka, Kunihiro. AU - Nakayamada, Shingo. AU - Ra, Chisei. AU - Tanaka, Yoshiya. PY - 2007/11/15. Y1 - 2007/11/15. N2 - Mast cells release many inflammatory mediators that play an important role not only in allergic diseases but also in chronic inflammatory diseases, autoimmune diseases, and others. A lot of mast cells exist in synovium of rheumatoid arthritis, and it is known that synovitis does not occur in mast cell-deficient mice. Thus, it is thought that mast cells play a very important role in rheumatoid arthritis pathogenesis. Leflunomide is a drug used clinically in the treatment of rheumatoid ...
Immune cells like NK cells, T cells, neutrophils and mast cells store high amounts of granule serine proteases, graspases. Graspases are encoded from the mast cell chymase locus. The human locus holds four genes: α-chymase, cathepsin G, and granzymes H and B. In contrast, the mouse locus contains at least 14 genes. Many of these belong to subfamilies not found in human, e.g. the Mcpt8-family. These differences hamper functional comparisons of graspases and of immune cells in the two species. Studies of the mast cell chymase locus are therefore important to better understand the mammalian immune system. In this thesis, the evolution of the mast cell chymase locus was analysed by mapping the locus in all available mammalian ...
Exposure to monomeric IgE in vitro markedly upregulated the ability of mature mouse peritoneal mast cells or mouse BMCMCs or cloned mast cells to bind IgE. Two separate lines of evidence indicate that this response largely, if not entirely, reflected the increased surface expression of FcεRI. First, while mouse mast cells also express FcγRII/ III ((17), (18)), which can bind IgE immune complexes ((18)), virtually all of the binding of monomeric IgE to mouse mast cells that is detectable under the conditions used in our experiments reflects binding of the ligand to a single class of high affinity binding sites, i.e., FcεRI ((18)). Second, we used anti-IgE to immunoprecipitate surface-bound IgE, and associated IgE receptors, from lysates of BMCMCs that had been first incubated with or without ...
TY - JOUR. T1 - Progress in allergy signal research on mast cells. T2 - Signal regulation of multiple mast cell responses through FcεRI. AU - Yamasaki, Shou. AU - Saito, Takashi. PY - 2008/4/8. Y1 - 2008/4/8. N2 - The crosslinking of FcεRI by IgE and antigen (Ag) on mast cells initiates activation cascades that lead to allergic responses. Although it was thought that IgE binding to FcεRI is a passive "sensitization", recent reports suggest that IgE actively promotes mast cell survival in the absence of Ag. However, it is largely unknown how these distinct responses are delivered through the same receptor, FcεRI, depending on the types of stimli. As an underlying molecular mechanism for the generation of diverse responses through FcεRI, we found that the quantity and the duration of the signal through the FcεRI γ chain (FcRγ) ...
In this study the diversity of mast cell proteases and some of the factors regulating mast cell growth and protease expression were examined in rodents. Five proteases were isolated from mouse small intestinal mucosa and their substrate specificities defined. The isolated proteases were all of mast cell origin and were chymotrypsin-like in their substrate specificities. The proteases were all identified as variants of mouse mast cell protease-1 which differed only in their carbohydrate moieties. Despite the fact that these enzymes shared a common core polypeptide they all differed significantly in the rate at which they hydrolysed synthetic substrates and in the rates at which they were inhibited by α1-proteinase inhibitor. A related, but distinct protease was isolated from peritoneal cavity mast cells of mice. This enzyme, ...
Mast cells are best known for their function in hypersensitive reactions, where aggregation of FcRI leads to the release of mast cell mediators leading to hypersensitive symptoms. although activation-induced success is certainly suffered, suggesting a minimal function for Bcl-XL, Bcl-2, Mcl-1 and Bcl-w. Reducing but not really amounts by siRNA inhibited activation-induced mast cell success. We also demonstrate that mast cell phrase of Bfl-1 is certainly raised in birch-pollen-provocated epidermis and in lesions of atopic dermatitis and psoriasis sufferers. Used jointly, our outcomes high light Bfl-1 as a main effector in activation-induced individual mast cell success. Launch Mast cells are known to end up being central regulators and effectors in allergic ...
Mast cell sarcoma is an extremely aggressive form of sarcoma made up of neoplastic mast cells. A sarcoma is a tumor made of cells from connective tissue. Mast cell sarcoma is an extremely rare tumor. Only 3 cases are reported so far. Prognosis is extremely poor. People with a mast cell sarcoma have no skin lesions, and pathology examination of the tumor shows it to be very malignant with an aggressive growth pattern. Mast cell sarcoma should not be confused with extracutaneous mastocytoma, a rare benign mast cell tumor without destructive growth. In the cases observed, mast cell sarcoma terminated quickly as mast cell leukemia; one of the most aggressive ...
By using the combination of reverse-transcription PCR and rapid amplification of cDNA ends methods, a cDNA encoding mast cell tryptase was successfully cloned from the small intestine of Mongolian gerbil, Meriones unguiculatus, infected with Nippostrongylus brasiliensis. The cDNA was 1219 bp long including 810 bp of an open reading frame. Based on the deduced amino acid sequences of known mast cell tryptases of other species, the gerbil mast cell tryptase (gMCT) was highly similar to mouse mast cell protease (mMCP)-7, and seems to be translated as a prepro-enzyme with 25 amino acids of signal and activation peptides and 245 amino acids of mature enzyme. The gMCT mRNA was preferentially transcribed in the intestinal mucosa and to a far lesser extent in the connective tissue such as skin and tongue. Moreover, kinetic study after infection revealed that the amount of gMCT mRNA ...
Tryptase (EC 3.4.21.59, ) is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation. Club cells contain tryptase which is believed to be responsible for cleaving the hemagglutinin surface protein of influenza A virus, thereby activating it and causing the symptoms of flu. Tryptase is also known by mast cell tryptase, mast cell protease II, skin tryptase, lung tryptase, pituitary tryptase, mast cell neutral proteinase, mast cell serine proteinase II, mast cell proteinase II, mast cell serine proteinase tryptase, rat mast cell protease II, and tryptase M. Serum levels are ...
Mutations in TGFBRI, encoding the receptor for TGFβ, cause Loeys-Dietz syndrome (LDS), a Mendelian disorder associated with an increased risk of developing nearly all forms of allergic disease, including food allergy. To better understand the mechanisms responsible for this association, knock-in mice harboring LDS mutations have been developed, which recapitulate nearly all aspects of the human phenotype. Mast cells are the main effector cells of allergic disease and several studies have identified TGFβ as a critical mediator of mast cell development and function. We hypothesized that LDS mast cells are altered in a manner that would promote greater susceptibility to allergic outcomes. To address this possibility, in vitro assays were performed with peritoneal mast ...
Five experiments were conducted comparing differential intestinal immune responses to two isolates of Eimeria acervulina (EA), EA1 and EA2. In three experiments, broiler chicks were divided into control (non-challenged), EA1, or EA2 challenged (14 days of age) groups. On day 6 post-challenge (PC), changes in body weight were determined, intestinal lesions were scored, and duodenal tissue was evaluated for morphometric alterations and mucosal mast cell responses. EA1 produced duodenal lesions and reduced villus height to crypt depth ratios when compared to controls; however, no differences were found in mast cell counts. EA2 produced differing results, and observed data were suggestive of an intestinal secretory response when compared to EA1 or controls. In Experiment 4, tissues were analyzed from day 2 through day 6 PC. Villus atrophy and crypt hyperplasia were heightened on day 5 PC in both challenged groups. Mast ...
The acidic granules of natural killer (NK) cells, T cells, mast cells, and neutrophils store large amounts of serine proteases. Functionally, these proteases are involved, e.g., in the induction of apoptosis, the recruitment of inflammatory cells, and the remodeling of extra-cellular matrix. Among the granule proteases are the phylogenetically related mast cell chymases, neutrophil cathepsin G, and T-cell granzymes (Gzm B to H and Gzm N), which share the characteristic absence of a Cys191-Cys220 bridge. The genes of these proteases are clustered in one locus, the mast cell chymase locus, in all previously investigated mammals. In this paper, we present a detailed analysis of the chymase locus in cattle (Bos taurus) and opossum (Monodelphis ...
Mast cells are responsible for the majority of allergic conditions. It was originally thought that almost all allergic events were mediated directly only via the high-affinity immunoglobulin E receptors. However, recent evidence showed that many other receptors, such as G protein-coupled receptors and ligand-gated ion channels, are also directly involved in mast cell degranulation, the release of inflammatory mediators such as histamine, serine proteases, leukotrienes, heparin, and serotonin. These mediators are responsible for the symptoms in allergic conditions such as allergic asthma. In recent years, it has been realized that purinergic signaling, induced via the activation of G protein-coupled adenosine receptors and P2Y nucleotide receptors, as well as ...
Methods: First of all, we injected histamine or anti-histamine agent-diphenhydramine into Zu Sanli (ST36) of the acute gastric injury mice model. As an indicator of the gastric emptying rate, we observe the histamine effects of acupuncture process at Zu Sanli (ST36). Next step, as indicator of the pain threshold change rate, we observe the acupuncture analgesia effect of histamine into Zu Sanli (ST36) on the mast cell deficiency mice.. Results: Injection of histamine into the point has a tendency to promote the gastric emptying rate of the gastric injury mice. But after injecting histamine into the acu-point, needling attenuated the acupuncture effect, and injecting anti-histamine agent -- diphenhydramine into the point, needling has no promotion effects on gastric emptying rate of the gastric injury mice. Injection of histamine into the ...
TY - JOUR. T1 - Inhibitory effect of nifedipine and cromolyn sodium on skin reactions and 45ca uptake and histamine release in rat mast cells induced by various stimulating agents. AU - Tanizaki, Yoshiro. AU - Akagi, Katsumi. AU - Lee, Kyung Nam. AU - Townley, Robert G.. PY - 1983. Y1 - 1983. N2 - The effect of nifedipine, one of the calcium channel antagonists, was examined in allergic and nonspecific reactions using rat skin and peritoneal mast cells. Rat passive cutaneous anaphylaxis response mediated by IgE antibody was inhibited by nifedipine (100 μg/rat) and also by cromolyn sodium (5 mg/rat). Skin reactions induced by histamine or methacholine were not inhibited, and skin reaction induced by serotonin was slightly inhibited by these drugs. The 45Ca uptake and histamine release in sensitized mast ...
Experimental autoimmune encephalomyelitis (EAE) is a mouse model that reproduces cardinal signs of clinical, histopathological, and immunological features found in Multiple Sclerosis (MS). Mast cells are suggested to be involved in the main inflammatory phases occurring during EAE development, possibly by secreting several autacoids and proteases. Among the latter, the chymase mouse mast cell protease 4 (mMCP-4) can contribute to the inflammatory response by producing endothelin-1 (ET-1). The aim of this study was to determine the impact of mMCP-4 on acute inflammatory stages in EAE. C57BL/6 wild type (WT) or mMCP-4 knockout (KO) mice were immunized with MOG(35-55) plus complete Freunds adjuvant followed by pertussis toxin. Immunized WT mice presented an initial acute phase characterized by progressive increases in clinical score, which were significantly reduced in mMCP-4 KO mice. In addition, higher levels ...
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Concern about the use of nanomaterials has increased significantly in recent years due to potentially hazardous impacts on human health. Mast cells are critical for innate and adaptive immune responses, often modulating allergic and pathogenic conditions. Mast cells are well known to act in response to danger signals through a variety of receptors and pathways including IL-33 and the IL-1-like receptor ST2. Here, the involvement of mast cells and the IL-33/ST2 axis in pulmonary and cardiovascular responses to multi-walled carbon nanotube (MWCNT) exposure are examined. Toxicological effects of MWCNTs are observed only in mice with a sufficient population of mast cells and are not observed when mast cells are absent or ...
This work reveals that an active form of human chymase can be captured by α2M, in which form it can cleave small peptide substrates, including angiotensin I, and is protected from irreversible inactivation by serpins and other antipeptidases in biological fluids. We exploited α2M binding to develop a sensitive and specific assay for chymase activity in the serum of subjects with mastocytosis. These studies reveal that chymase, after secretion by mast cells and capture by α2M, can cleave small peptides for longer than once thought possible. Extravascular chymase captured and protected by α2M may be an important source of non-ACE-generated angiotensin II near tissue sites of mast cell degranulation. The portion of α2M-caged chymase making its way to the bloodstream provides the basis of our serum assay and may be a mobile source of angiotensin II-generating chymase in blood and tissues remote from original ...
TY - JOUR. T1 - Effect of loratadine on histamine release induced by antigen-antibody reaction. AU - Kamei, Chiaki. AU - Sugimoto, Yukio. AU - Yamaji, Masako. AU - Takada, Miho. PY - 1996. Y1 - 1996. N2 - Loratadine caused an inhibition of histamine release from rat peritoneal mast culls induced by passively sensitized mast cells, and IC 50 was 9.57 μM. SCH 34117, a metabolite of loratadine, also inhibited histamine release from mast cells, and its potency was more than that of loratadine. Moreover, in ex vivo experiments, loratadine (5 mg/kg, p.o.) as well as terfenadine provided a relatively potent inhibitory effect on histamine release from lung pieces of actively sensitized guinea pigs exposed to antigen.. AB - Loratadine caused an inhibition of histamine release from rat peritoneal mast ...
This study assesses the activity and safety profile of twice-daily oral doses of midostaurin in patients with aggressive systemic mastocytosis (ASM) or mast cell leukemia (MCL) with or without associated clonal hematological non-mast cell lineage disease (AHNMD).. Aggressive systemic mastocytosis (ASM) and mast cell leukemia (MCL) are characterized by excessive bone marrow production of mast cells which can can infiltrate tissues and release harmful substances, resulting in organ damage. These diseases have very limited treatment options and poor prognosis. Existing treatments for in advanced mast cell disease, eg, interferon-alpha; corticosteroids; and/or cladribine, exhibit low response rates that are usually partial in nature. ...
TY - JOUR. T1 - Effective mast cell degranulating peptide inhibitors of the IgE/FcεRI receptor interaction. AU - Buku, Angeliki. AU - Keselman, Inna. AU - Lupyan, Dmitry. AU - Mezei, Mihaly. AU - Price, Joseph. PY - 2008/8/1. Y1 - 2008/8/1. N2 - Previous studies with mast cell degranulating (MCD) peptide have shown that peptide [Ala12]MCD 8 was an inhibitor of IgE binding to mast cell receptors. In an attempt to produce increased inhibition, analogs were synthesized that maintained the alanine residue in position 12 in the MCD peptide sequence and were further modified at both termini. Analogs modified at the C-terminus were [Ala12,desLys21]MCD 2 and [Ala 12,d-Lys21]MCD 4. N-terminus modifications were [desLys6-Arg7-His8,Ala12]MCD 1, [Ala6, Ala12]MCD 6, and [Val6,Ala 12]MCD 7. To assess the role of the Proline12, analogs [d-Ala12]MCD 3 and [Meleu12]MCD 5 were also synthesized. The analogs ...
TY - JOUR. T1 - Mast cell growth factor (c-kit ligand) enhances cytokine stimulation of proliferation of the human factor-dependent cell line, M07e. AU - Hendrie, P. C.. AU - Miyazawa, K.. AU - Yang, Y. C.. AU - Langefeld, C. D.. AU - Broxmeyer, Hal. PY - 1991. Y1 - 1991. N2 - Murine mast cell growth factor (muMGF), a c-kit ligand, has additive to greater-than-additive effects on in vitro colony formation of murine and human myeloid progenitor cells stimulated with erythropoietin, granulocyte-macrophage colony-stimulating factor (GM-CSF), and/or interleukin (IL)-3. To confirm direct-acting effects on responding cells, MGF was assessed alone and in combination with other cytokines for effects on the proliferation of the human factor-dependent cell line, M07e. Proliferation was assayed in liquid culture by [3H]thymidine uptake and in semisolid ...
Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional ...
Mast Cell Mediator Symptoms. The myriad symptoms patients with mast cell disorders experience during mast cell activation can wreak havoc on patients on a daily basis, and multiple organ systems, including pulmonary, cardiovascular, dermatologic, gastrointestinal, musculoskeletal, and neurologic can be involved. Table 1 lists some potential effects linked to specific mediators.5-13 Symptoms (Table 2) may include, but are not limited to: flushing of the face, neck, and chest; headache; tachycardia and chest pain; abdominal pain, bloating, gastroesophageal reflux disease (GERD), diarrhea, vomiting; uterine cramps or bleeding; rashes, including maculopapular cutaneous mastocytosis (MPCM)/urticaria pigmentosa (UP), telangiectatic lesions; bone/muscle pain, osteosclerosis, osteopenia, osteoporosis; itching, +/- rash; blood pressure instability; brain fog, cognitive dysfunction; ...
Parekh, A. B.; Penner, R.: Activation of store-operated calcium influx at resting InsP(3) levels by sensitization of the InsP(3) receptor in rat basophilic leukaemia cells. Journal of Physiology-London 489 (2), pp. 377 - 382 (1995 ...
The therapeutic effect of mitochondria-targeted antioxidant 10-(6´-plastoquinonyl)decyltriphenylphosphonium bromide (SkQ1) in experimental models of acute inflammation and wound repair has been shown earlier. It was suggested that the antiinflammatory activity of SkQ1 is related to its ability to suppress inflammatory activation of the vascular endothelium and neutrophil migration into tissues. Here, we demonstrated that SkQ1 inhibits activation of mast cell (MCs) followed by their degranulation and histamine release in vivo and in vitro. Intraperitoneal injections of SkQ1 in the mouse air-pouch model reduced the number of leukocytes in the air-pouch cavity and significantly decreased the histamine content in it, as well as suppressing MC degranulation in the air-pouch tissue. The direct effect of SkQ1 on MCs was studied in vitro in the rat basophilic leukemia RBL-2H3 cell line. SkQ1 inhibited induced ...
The effect of Ephedrae herba(Mao) on histamine content was investigated. When rat basophilic leukemia (RBL-2H3) cells were incubated for 48 h with Mao, Mao decreased histamine content in a...
The fact that psychiatric symptoms occur as a function of mast cell disease on the nervous system is common knowledge to patients but less acknowledged by providers. A significant population of mast cells is found in the brain in close association with both blood vessels and nerve cells. Mast cells are present in large numbers in the hypothalamus, which regulates stress response, emotion and cognition; the amygdales, near the pituitary gland; and the thalamus. Lesions and structural changes in the thalamus have previously been associated with altered perception of pain and emotional reactivity.. One study found that in a group of 88 patients with indolent systemic mastocytosis (ISM) and cutaneous mastocytosis (CM), 75% reported depressive symptoms. In another study, a group of 288 ...
Histopathology, with the associated pathology report will give your veterinarian the diagnosis of tumor type and status that helps to indicate how the tumor is likely to behave. The veterinary pathologist usually adds a prognosis that describes the probability of local recurrence or metastasis (distant spread).. Well-differentiated, benign, solitary mast cell tumors are cured by surgical removal, even in elderly cats. However, there is a tendency for cats to develop multiple, apparently primary, sequential mast cell tumors, some of which are benign and some not. Multiple tumors disappear within a few months in young cats but the situation in older cats is less certain.. Some feline mast cell tumors have features indicating potential malignancy. Multiple tumors are common and the tumors usually recur or spread to other sites in 2 to 3 months. Tumors often involve the lymph nodes but do not necessarily spread ...
41. Can my mast cell disease go away? Will it ever not be a problem?. There are several common questions that basically all distill down to these sentiments. Im going to answer them all here.. I have previously answered the question "Can mast cell disease be cured?" in this series but I think this question is a little different. When people ask if mast cell disease can go away, they mean can it become no longer a problem even if its not cured. Thats what Im answering here.. This answer is very complicated so Im just going to give my thoughts lets about all sides of this situation.. Yes, it is possible for mast cell disease to be controlled enough to no longer be a problem in your life. But there are a lot of caveats.. The most common presentation of mast cell disease in cutaneous mastocytosis ...
Mast cell tumors (MCT) represent a cancer of mast cells, which are a type of white blood cell involved in allergy and inflammation. Mast cell tumors are the most common skin tumor in dogs. Older dogs are most commonly affected. Some predisposed breeds of dogs include Boxers, Boston terriers, Bulldogs, pugs, Labrador retrievers, golden retrievers, Weimaraners, Rhodesian Ridgebacks, cocker spaniels, schnauzers, Staffordshire Terriers, beagles and Shar Peis.
The discovery that the high affinity IgE receptor (Fc epsilon RI) is expressed on APCs of patients with atopic diseases raised the possibility that the functional importance of Fc epsilon RI in the pathogenesis of atopy may extend beyond its role in type I allergic reactions. Here we show that, following removal of in vivo-bound IgE by lactic acid treatment, targeting of allergens to monocytes by Ag-specific IgE critically depends on Fc epsilon RI expression. Even more importantly, lactic acid-treated, monocyte-enriched PBMCs present allergen to T cells 100- to 1000-fold more effectively if the allergen has been targeted to Fc epsilon RI on these cells via allergen-specific IgE. This mechanism may critically lower the atopic individuals threshold to mount allergen-specific T cell responses capable of promoting IgE production and delayed-type hypersensitivity reactions. ...
Intracellular expression of Ab fragments has been efficiently used to inactivate therapeutic targets, oncogene products, and to induce viral resistance in plants. Ab fragments expressed in the appropriate cell compartment may also help to elucidate the functions of a protein of interest. We report in this study the successful targeting of the protein tyrosine kinase Syk in the RBL-2H3 rat basophilic leukemia cell line. We isolated from a phage display library human single-chain variable fragments (scFv) directed against the portion of Syk containing the Src homology 2 domains and the linker region that separates them. Among them, two scFv named G4G11 and G4E4 exhibited the best binding to Syk in vivo in a yeast two-hybrid selection system. Stable transfectants of RBL-2H3 cells expressing cytosolic G4G11 and G4E4 were established. Immunoprecipitation experiments showed that intracellular ...
Crosslinking of Fc epsilon RI on rat basophilic leukemia (RBL 2H3) cells leads to an increase in Phosphatidylinositol 4-kinase activity. This increase in Ptdlns 4-kinase activity is strongly correlated with its tyrosyl phosphorylation state. Characterization of the enzyme activity in anti phosphotyrosine immunoprecipitates suggests it as a type II Ptdlns 4-kinase. Membrane cholesterol depletion studies showed a reduction in type II Ptdlns 4-kinase activity suggesting that lipid rafts play an important role in activation of the enzyme. The enzyme activity was inhibited by resveratrol. In situ inhibition of type II Ptdlns 4-kinase activity showed a reduction in beta-hexosaminidase release upon Fc epsilon RI cross-linking. These studies suggest that a type II Ptdlns 4-kinase is an integral component of Fc epsilon RI mediated signal transduction mechanisms. (c) ...
Looking for online definition of basophilic aggregation test in the Medical Dictionary? basophilic aggregation test explanation free. What is basophilic aggregation test? Meaning of basophilic aggregation test medical term. What does basophilic aggregation test mean?
View list of generic and brand names of drugs used for treatment of Mastocytosis (Rare Diseases - Excess Mast Cell Production ). You can find more information including dosage, side effects of the Mastocytosis (Rare Diseases - Excess Mast Cell Production ) medicine.
Distinct from mastocytosis and simple allergy and characterized by constitutive mast cell (MC) activation and aberrant MC reactivity with little to no excessive MC accumulation, MC activation syndrome (MCAS) presents as acute-on-chronic multisystem polymorbidity of generally inflammatory ± allergic theme and may be epidemically prevalent (PLoS ONE 2013;8(9):e76241). Occasional patients (pts) suffer nearly continuous anaphylactoid and/or dysautonomic states poorly controlled by intermittently dosed epinephrine (epi), antihistamines, steroids, etc. Many medications (meds) have helped MCAS pts, but there are not yet any biomarkers predictive of effective therapy. Pts often try many meds, but med trials in pts suffering very frequent flares are impractical in that a flaring soon after dosing of a new med cannot be distinguished from "baseline" flaring from yet-uncontrolled disease vs. flaring in specific ...
Pain is something that doesnt get talked about enough when you have Mast Cell Activation Syndrome and Histamine Intolerance, but is incredibly common.
Acute basophilic leukemia is a rare form of acute myeloid leukemia where blasts are accompanied by abnormal basophils in all stages of differentiation. It would most likely be classified as M0 without electron microscopic confirmation of basophil lineage. Differentiated (basophilic granules by light microscopy) and poorly differentiated cases ; Majority are poorly differentiated. MPO negative by light microscopy; granules positive in a speckled pattern by electron microscopy. Myeloid antigens are expressed. Diagnosis of poorly differentiated cases made by electron microscopy. May manifest basophil and mast cell granules by EM. Cytogenetically heterogeneous but frequently associated with Philadelphia chromosome. There is no clinically distinguishing features but may be more common in children and young adults and carry a poor prognosis. Liu, P.P.; A. Hajara; C. Wijmengac; F.S. Collins (1995). "Molecular pathogenesis of chromosome 16 inversion ...
Definition of basophilic line in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is basophilic line? Meaning of basophilic line as a finance term. What does basophilic line mean in finance?
Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain-a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins-targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling networks. ...
Staal-Viliare A, Latger-Cannard V, Rault JP, Didion J, Grégoire MJ, Bologna S, Witz B, Jonveaux P, Lecompte T, Rio Y: [A case of de novo acute basophilic leukaemia: diagnostic criteria and review of the literature]. Ann Biol Clin (Paris); 2006 Jul-Aug;64(4):361-5 ...
Mast cells play a central role in the pathogenesis of allergic reaction. Activation of mast cells by antigens is strictly dependent on the influx of extracellular calcium that involves a complex interaction between signalling molecules located within the cells. We have previously reported that tHGA, an active compound originally isolated from a local shrub known as Melicope ptelefolia, prevented IgE-mediated mast cell activation and passive systemic anaphylaxis by suppressing the release of interleukin-4 (IL-4) and tumour necrosis factor (TNF)-α from activated rat basophilic leukaemia (RBL)-2H3 cells. However, the mechanism of action (MOA) as well as the molecular target underlying the mast cell stabilising effect of tHGA has not been ...
The chemokinetic inhibitory factor (CIF) is a recently described B-cell derived lymphokine that mediates a chemokinetic inhibitory effect on human polymorphonuclear leukocyte (PMN) migration. In the present report the interaction of CIF with the neutrophil plasma membrane was studied. Normal human peripheral blood neutrophils and purified neutrophil plasma membranes selectively removed biologic activity from CIF-containing concentrates obtained during the purification procedure from conditioned medium. Removal was obtained at both 4 degrees C and 37 degrees C. Furthermore, HL-60 cells treated with dimethyl sulfoxide removed CIF activity (granulocyte-like cells) but HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (macrophage-like cells) did not. Purified human blood monocytes, cells from the ...
The dendritic cell (DC) lineage encompasses a diverse population of cells with unique subtype-specific functions. In peripheral blood, four DC subsets have been identified based on their distinct expression of CD1c, CD141, CD16, and CD123, and these subpopulations exhibit functional properties in immune responses. However, their respective roles in allergic diseases, such as rhinitis, are unclear. In this study, we have performed comparative assessments of DC subset frequencies and investigated their Fc epsilon RI expression levels in patients with allergic rhinitis. We demonstrate that the frequencies of CD1c(+) and CD141(+) DCs are elevated in grass pollen-allergic subjects compared with healthy controls, irrespectively of allergen stimulation. Among the DC subsets, CD1c(+) DCs expressed the highest levels of Fc epsilon RI mRNA, and a large proportion expressed surface Fc epsilon RI. Furthermore, the Fc ...
The purpose of this study was to evaluate the role of two major allergic pathways - IgE and non-IgE antibody mediated food allergy reaction (FAR) and lung inflammation disease using crustacean extracts. Although IgE antibody mediated food allergy reaction (IFAR) are defined as major mechanism in the past several decades, proteases from different sources acting similar to allergens as well as the long-term inflammatory stimulus through activation of protease activation receptors (PARs) are important. This opens new insight into non-IgE antibody mediated food allergy (NFAR) due to its dual effects. Therefore, it is necessary to investigate the interactions of these two pathways for a better understanding of the currently poor correlation between clinical symptoms and IgE specific diagnostics.. The current studies employed a humanized rat basophilic leukemia (RBL) cell line as well as a human lung epithelial cell line (A549) ...
Resveratrol (RSV), a natural polyphenol found in grapes and other herbal plants, has been reported to possess anti‑inflammatory, anti‑oxidative and anti‑proliferative activities. The aim of the present study was to investigate the effect of RSV on interleukin (IL)‑33‑induced inflammatory responses in mast cells and identify the underlying molecular mechanisms. Rat basophilic leukemia (RBL‑2H3) cells were stimulated with IL‑33 in the presence or absence of RSV. MTT, ELISA, reverse transcription‑quantitative PCR and western blot analyses were then performed in order to assess cytotoxicity, inflammatory cytokine production, suppression of tumorigenicity 2 receptor expression, protein expression involved in mitogen‑activated protein kinase (MAPK) and nuclear factor (NF)‑κB signaling, respectively. Finally, rats were used to determine the biological effect of RSV in vivo. The results revealed ...
The aim of this study was to investigate the influence of the β chain of the high affinity IgE receptor on the development of the atopic phenotype, as postulated by Cookson et al.8 9 11 In order to make the comparison more valuable, we have chosen the same phenotypes for the study as the group from Oxford, we used the same microsatellite probes for linkage analysis, and checked for the same polymorphisms. We studied two populations, one recruited through a population based study and the other through our outpatient department. Thus, the much higher number of clinically affected children in the second population (45.8% versus 5.4%) was expected. Assuming that atopy represents a genetically heterogeneous disease, it might well be associated with a different set of genes involved or a different degree of involvement of each gene in our populations.. We could not find definite evidence for involvement of the the β chain of the high affinity IgE receptor on enhanced IgE responsiveness in a ...
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FINAL DIAGNOSIS:. MOTOR NEURON DISEASE WITH BASOPHILIC INCLUSIONS. DISCUSSION:. Motor neuron disease (MND) is associated with a number of intracellular inclusion bodies within surviving motor neurons. In both sporadic and familial MND, Bunina bodies, skein-like inclusions and hyaline inclusions, some of which resemble Lewy bodies, may be seen (1). In Guam-type MND (2), post encephalitis lethargica-related MND (3) and occasional cases of familial MND with mutations in the SOD-1 gene (4), neurofibrillary tangles have been demonstrated. In addition, there have been a few reports (mainly in young individuals) of MND with basophilic cytoplasmic inclusions (5-11). MND with basophilic inclusions appears to be a distinctive histopathological subtype, characterized by circumscribed intraneuronal basophilic inclusions within motor neurons, but also widely distributed within the cerebral cortex, basal ganglia, brainstem and cerebellar nuclei. Unlike the more common MND-inclusions, these basophilic ...
Identification of basophilic meta chromatic cells (BMCS) and relationships between BMCS, bacteria in nasal cavity and specific immunoglobulin E against house dust mites with the severity of allergic rhinitis / Yuwatida ...
The Ins(1,4,5)P3 regioisomers, Ins(1,4,6)P3 and Ins(1,3,6)P3, which can mimic the 1,4,5-arrangement on the inositol ring of Ins(1,4,5)P3, were examined for Ca2+ release by using four types of saponin-permeabilized cell possessing various abundances of receptor subtypes, with special reference to the relation of potency to receptor subtype. Ins(1,4,6)P3 and Ins(1,3,6)P3 were weak agonists in rat basophilic leukaemic cells (RBL cells), which possess predominantly subtype II receptors, with respective potencies of 1/200 and less than 1/500 that of Ins(1,4,5)P3 [the EC50 values were 0.2, 45 and more than 100 microM for Ins(1,4,5)P3, Ins(1,4,6)P3 and Ins(1,3,6)P3 respectively]. Similar rank order potencies were also evaluated for the displacement of [3H]Ins(1,4,5)P3 bound to RBL cell membranes by these regioisomers. However, they caused Ca2+ release from GH3 rat pituitary ...
Background Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+(CRAC) channels, promoting an immune response to pathogens. Defects in a CRAC (Orai) channel in...
Description: Rat monoclonal anti-IgE antibodies that was generated by fusion of plasmacytoma (84.1C) or myeloma (EM953) cells with splenocytes of rat immunized with purified murine IgE mAb. The antibodies react with various IgE mAb of different specificities and not with immunoglobulins of other classes, and recognize an epitope on the murine Fc epsilon region. Were shown to block IgE-Fc?R interactions and inhibit passive cutaneous anaphylaxis. Clone 84.1c recognizes a site on IgE, which is identical or very close to the Fc?R binding site. May be used for detection and manipulation of the IgE response in mice. Reference: Schwarzbaum S, Nissim A, Alkalay I, Ghozi MC, Schindler DG, Bergman Y, Eshhar Z. 1989. Mapping of murine IgE epitopes involved in IgE-Fc epsilon receptor interactions. Eur J Immunol 19(6):1015-23. M182, M185, M186. ...
MS4A2 of 244 aas and 4 TMSs. High affinity receptor that binds to the Fc region of immunoglobulins epsilon. Aggregation of Fc epsilon receptor (FCERI) by multivalent antigens is required for the full mast cell response, including the release of preformed mediators (such as histamine) by degranulation and de novo production of lipid mediators and cytokines (Penhallow et al. 1995). Also mediates the secretion of important lymphokines. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators responsible for the manifestations of allergy. ...
Urticaria is a group of disorders that share a distinct skin reaction pattern, namely the occurrence of itchy wheals anywhere on the skin. Wheals are short-lived elevated erythematous lesions ranging from a few millimetres to several centimetres in diameter and can become confluent. The itching can be pricking or burning and is usually worse in the evening or night time [1]. Nearly half of the patients report sleep disturbances [2]. Typically, the lesions are rubbed and not scratched; therefore, usually excoriated skin is not a consequence of urticaria. Itchy wheals and also angioedema, which occur in about half of the patients from time to time, are the result of the degranulation of mast cells and basophils with release of mediators, predominantly histamine. Possible direct and indirect mechanisms by which degranulation is induced include autoreactivity including autoimmunity mediated by functional autoantibodies directed against the high ...
The allergic response involves the binding of allergen to receptor-bound IgE followed by cell activation and the release of mediators responsible for the manifestations of allergy. The IgE-receptor, a tetramer composed of an alpha, beta, and 2 disulfide-linked gamma chains, is found on the surface of mast cells and basophils. This gene encodes the beta subunit of the high affinity IgE receptor which is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This family member is localized to 11q12, among a cluster of membrane-spanning 4A gene family members. Alternative splicing results in multiple transcript variants encoding distinct proteins. Additional transcript variants have ...
With multiple species, Fc epsilon RI cDNA ORF Clone, Mouse in pGEM-T Vector is confirmed by Full-length sequence verification, in stock
Other than a role for Ca2+ store depletion, the molecular mechanisms that regulate antigen-stimulated Ca2+ influx into mast cells are not well-understood. The observation that CT dramatically enhances 45Ca2+ influx into RBL-2H3 cells suggests that this reagent might be a useful tool to study the Ca2+ entry pathway (Narasimhan et al., 1988). That CT amplifies both antigen-evoked ICRAC and 45Ca2+ influx to a similar extent bolsters the idea that CRAC channels are a major pathway for FcεRI-mediated Ca2+ uptake into RBL-2H3 mast cells (Zhang and McCloskey, 1995).. Two hypotheses to explain the effect of CT on 45Ca2+ influx are immediately testable by patch clamping. First, it is possible that CT activates Cl− or K+ channels, and thereby increases the electrical force propelling Ca2+ entry. This indirect mechanism cannot explain the enhancement of Ca2+ influx currents that we ...
II.E.001. Normal basophil. Blood film. The field contains a basophil and lymphocyte. The dark blue basophilic granules characteristically obscure the nucleus. The granules are relatively large and easily resolved with the light microscope. In this case some cytoplasmic basophilic granules were lost in film preparation and the cell is partially degranulated as a result of the trauma of preparation. This finding is common in blood films.. ...
Define basophil: a basophilic substance or structure; especially : a white blood cell containing basophilic granules that is similar in function to a…
To explore the clinical significance of genetic detection and changes of red cell enzyme activities of pyrimidine 5 nucleotidase (P5N), pyruvate kinase (PK) and glucose-6-phosphate dehydrogenase (G-6-PD) in patients with α-thalassaemia (α-thal). Three α-thal patients were further processed to gene detection by PCR-trans-dot blot and gap-PCR, and red cell enzymes ...
A) On a Wright stained peripheral blood smear, those cells have a faint bluish shade within the cytoplasm (polychromatophilia) that displays staining of residual messenger RNA directing the synthesis of hemoglobin. those cells can also include punctate blue staining, known as basophilic stippling, which represents staining of brought about ribosomes. Basophilic stippling is mostly visible while theres irregular heme or globin synthesis as obvious within the microcytic anemias (see bankruptcy 4). it might even be visible whilst theres irregular bone functionality as within the myelodysplastic syndromes (see bankruptcy 18).. G. , rituximab) or mixture of those brokers. such a lot havent been without delay in comparison. just like indolent lymphoma, reaction premiums increase whilst rituximab is mixed with preliminary chemotherapy. a call among those cures is made established upon sufferer features and targets of treatment. ...
The British Society for Haematology is registered in England and Wales as a Company Limited by Guarantee, No 2645706 and as a Charity, No 1005735 Registered Office and correspondence address: 100 White Lion Street London N1 9PF. Phone: 020 7713 0990 ...
Among the cattle admitted to the II. Medizinische Tierklinik of the University of Munich during the years 1982-86, 38 calves of up to 6 weeks of age had a dystrophy (n=5), pre-cirrhosis (n=21) or cirrhosis (n=12) of the liver. Similar hepatic lesions were found in three 1- to 7-day-old calves ...
Sigma-Aldrich offers abstracts and full-text articles by [Nitasha R Bennett, Daniel B Zwick, Adam H Courtney, Laura L Kiessling].
Armenian hamster monoclonal Fc epsilon RI antibody [MAR-1] validated for IP, IHC, FuncS, Flow Cyt and tested in Mouse. Immunogen corresponding to the details…
Quantity100 testsVolume0.4ImmunogenEBV-transformed human cellsBackground InformationCD23 (Fc ε RII), the low affinity IgE receptor, is a 45 kDa typ...
Observation of fluorescently labeled IgE receptors and Lyn-EGFP kinase on RBL mast cells. (A) Schematic showing the transmembrane receptor, FcɛRI (purple), IgE
Complete information for RBL2 gene (Protein Coding), RB Transcriptional Corepressor Like 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Of clinical interest is also the quantification of the histamine release from basophilic leucocytes in allergies (please refer to the add-on kit Histamine Release; reference no. BA E-1100).. ...
This is a basophil (white blood cell or granular leukocyte) which is moderately advanced. Its diameter is approximately 10 micrometers. Again the basophilic granules tend to obscure the shape of the nucleus, however, there is a suggestion that the nucleus is, at this stage, bilobed. Wrights stain. x2150. - Stock Image P248/0294
Cells with conspicuous eosinophilic cytoplasm and centrally located nuclei (sometimes paired) are the parietal cells (P in the figure above).. Cells with basal nuclei, basally basophilic cytoplasm, and apical vesicles are the chief cells (C in the figure above).. The glands are separated by thinner strands of lamina propria, visible as strands of denser, flattened or irregular nuclei. ...
Common variant: intermediate-sized lymphocyte, high N/C ratio, round or irregular nuclear contour, moderately condensed chromatin, prominent central nucleoli, deeply basophilic cytoplasm with protrusions, no ...
BioAssay record AID 785333 submitted by ChEMBL: Open TG-GATES: Regimen: Daily Repeat; Time: 4 day; Dose: High; Route: Gavage | Dataset: Pathology; Tissue: Kidney; Assay: Tubular Epithelium, Change, Basophilic; Study_ID: 172/13.
Huang EJ, Zhang J, Geser F, Trojanowski JQ, Strober JB, Dickson DW, Brown RH, Shapiro BE, Lomen-Hoerth C. Extensive FUS-immunoreactive pathology in juvenile amyotrophic lateral sclerosis with basophilic inclusions ...
BADAN HOWELL-JOLLY Badan ini adalah sisa inti yang mengandung DNA.Ukurannya sekitar 1-2u. dapat ditemukan tunggal atau ganda, letaknya ekstentrik di dekat membran eritrosit. Dapat dijumlai pada: pasca splenektomi thalassemia anemia hemolitik anemia megaloblastik hiposplenia fungsional TITIK-TITIK BASOFIL (BASOPHILIC STIPPLING) Titik basofil ditemukan di dalam eritrosit sebagai titik-titik kecil berwarna biru tua (basofilik),diduga merupakan sisa RNA…
Dot plots showing immunoreactivity pattern of blast cells. Basophilic cells account for 38% of the total nucleated cells. Blast cells showing positivity for (A)
We are analyzing the initial molecular events stimulated by the high-affinity receptor for IgE, FcR I, one of the multichain immune response receptors that play a central role in the initiation and regulation of immune responses. When a multivalent antigen interacts with specific IgE firmly bound to the receptor, the aggregated FcRI initiate a cascade of biochemical events.
cyclo(L-262): inhibits the interaction between immunoglobulin E (IgE) and its high affinity receptor, Fc epsilon RI; structure given in first source
RBL Champion E Tha Real vs. Alamo IV 10-14-2005 Alamo IV RBL wow . . E the Champ?, u nothing but a faggot scrub, Caz Im known to blow up on E...
WELCOME TO WEEK 6 OF THE RBL HEAVYWEIGHT DIVISION Heavyweight Champion Severity 5-0 Vs. Heavyweight #1 Contender Allen KNight 3-0 #3 Coup...
Summary of RBL1 (cp107, p107, PRB1) expression in human tissue. Nuclear and cytoplasmic expression in several different tissue types, including lymphoid tissue.
Los Angeles, California, U.S.A. vacations: find accommodation, things to do, maps, attractions, hotels, photos, flights, weather, travel advice and more.
Montana Fish, Wildlife & Parks and the Idaho Department of Fish and Game will intervene if a threatened lawsuit is filed against the U.S. Fish and Wildlife Service and its recent decision not to list the westslope cutthroat trout as a threatened species, FWP officials announced today.. Montanas successful and on-going efforts to protect and restore native westslope cutthroat trout has made it unnecessary to add Montanas state fish to the nations growing list of endangered species, said FWP director Pat Graham. Montanas cutthroat trout restoration effort is a model of species protection, planning, action, and tireless commitment to a natural resource preservation effort that is producing real-life results. The recovery and protection of our state fish will continue to be one of the major conservation efforts of this agency, our governor, dozens of landowners and many state, federal and tribal cooperators.. Last week, some Montana-based conservation interests challenged the U.S. Fish and ...

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Mast cell function has been dissected in detail with the use of rat basophilic leukemia mast cells (RBL-2H3), a widely accepted ... Mast cell granule component and the allergic mediator β-hexosaminidase, which is released linearly in tandem with histamine ... where it binds to GABAA receptors (GABAARs) present at the plasma membrane of the synapse-receiving, postsynaptic neurons. ... cells and T cells1. Unlike classical T cells, NKT cells recognize lipid antigen in the context of CD1 molecules2. NKT cells ...
more infohttps://www.jove.com/visualize/abstract/24223188/identification-diversity-killer-cell-ig-like-receptors-aotus

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Mast cell function has been dissected in detail with the use of rat basophilic leukemia mast cells (RBL-2H3), a widely accepted ... Mast cell granule component and the allergic mediator β-hexosaminidase, which is released linearly in tandem with histamine ... thyrocytes and C-cells differentiate, and (iv) endothelial cells present in the microdissected tissue proliferate, migrate into ... Medicine, Issue 88, rat brain endothelial cells (RBEC), mouse, spinal cord, tight junction (TJ), receptor-mediated transport ( ...
more infohttps://www.jove.com/visualize/abstract/21209948/pge2-induces-il-6-orbital-fibroblasts-through-ep2-receptors-increased

Mast cell - WikipediaMast cell - Wikipedia

... including phospholipase Cγ1 and the receptor βγ2 complex, in RBL-2H3 rat basophilic leukemia cells". Molecular and Cellular ... and allergic rhinitis and allergic conjunctivitis. Antihistamine drugs act by blocking histamine action on nerve endings. ... The activities of the latter are dependent on T-cells. Mast cells are present in most tissues characteristically surrounding ... Other neoplastic disorders associated with mast cells include mast cell sarcoma and mast cell leukemia. Mast cell activation ...
more infohttps://en.wikipedia.org/wiki/Mast_cell

Thymus - WikipediaThymus - Wikipedia

The ability of T cells to recognize foreign antigens is mediated by the T-cell receptor. The T-cell receptor undergoes genetic ... Allergies involve mainly IgE, antibodies, and histamine. Mast cells release the histamine. Sometimes an allergen may cause a ... Patients may present with a profound immunodeficiency disease, due to the lack of T cells. No other immune cell lineages are ... Lymphomas or leukemias of thymocyte origin are classified as Precursor T acute lymphoblastic leukemia/lymphoma (T-ALL). People ...
more infohttps://en.wikipedia.org/wiki/Thymus

Antibodies  | Free Full-Text | Role and Redirection of IgE against Cancer | HTMLAntibodies | Free Full-Text | Role and Redirection of IgE against Cancer | HTML

Because of its peculiar immune features, IgE may present a superior anti-tumor performance as compared to IgG. However, extreme ... A common doubt is based on whether IgE mode of action should only be relegated to anti-parasite immunity and allergic ... Tryptase-positive mast cells correlate positively with bone marrow angiogenesis in B-cell chronic lymphocytic leukemia. ... of the FcεRI-bound antigen-specific IgE and the consequent receptor activation leads to the early phase of the allergic ...
more infohttp://mdpi.com/2073-4468/2/2/371/htm

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Mast cell function has been dissected in detail with the use of rat basophilic leukemia mast cells (RBL-2H3), a widely accepted ... Mast cell granule component and the allergic mediator β-hexosaminidase, which is released linearly in tandem with histamine ... primary CD4+ T cells from pre-diabetic NOD mice transgenic for the islet-specific T cell receptor (TCR) BDC2.5 into NOD.SCID ... Here we present a method for the efficient preparation of hepatic stellate cells from mouse liver. Due to their perisinusoidal ...
more infohttps://www.jove.com/visualize/abstract/23755289/cooperative-anti-diabetic-effects-deoxynojirimycin-polysaccharide

IgE Enhances Mouse Mast Cell FcεRI Expression In Vitro and In Vivo: Evidence for a Novel Amplification Mechanism in IgE...IgE Enhances Mouse Mast Cell FcεRI Expression In Vitro and In Vivo: Evidence for a Novel Amplification Mechanism in IgE...

1985) The receptor for immunoglobulin E on rat basophilic leukemia cells: Effect of ligand binding on receptor expression. Proc ... Compared with mast cells that had been incubated with levels of monomeric IgE similar to those present in the sera of some ... and antigen-specific mast cell activation and mediator production is thought to be critical to the pathogenesis of allergic ... Receptor cross-linking and histamine release: the quantitative dependence of basophil degranulation on the number of receptor ...
more infohttp://jem.rupress.org/content/185/4/663

Nicotinic Acid Receptors | Small Molecule Inhibitors of Statin-Induced APP C-terminal Toxic Fragment ProductionNicotinic Acid Receptors | Small Molecule Inhibitors of Statin-Induced APP C-terminal Toxic Fragment Production

CD22 antigen is present in the most B-cell leukemias and lymphomas but not T-cell leukemias. In contrast with CD10, CD19 and ... The fact that mast cells accumulate at sites of inflammation, such as the nasal mucosa of patients with allergic rhinitis [4], ... histamine, cytokines, and chemokines [1,2]. Mast cells have been implicated in acute and chronic inflammatory responses and in ... Category Archives: Nicotinic Acid Receptors. Nicotinic Acid Receptors Background Individual mast cells can handle a multitude ...
more infohttp://ivachtin.com/category/nicotinic-acid-receptors/

Ala<sup>12</sup>]MCD peptide: A lead peptide to inhibitors of immunoglobulin E binding to mast cell...Ala<sup>12</sup>]MCD peptide: A lead peptide to inhibitors of immunoglobulin E binding to mast cell...

... inhibition of IgE binding to the FcεRIα mast cell receptor by using rat basophilic leukemia (RBL-2H3) mast cells and ... bind with high affinity to mast cell receptors without triggering secretion of histamine or other mediators of the allergic ... The present results show that [Ala12]MCD may provide a base for designing agents to prevent IgE/FcεRIα interactions and, ... inhibition of IgE binding to the FcεRIα mast cell receptor by using rat basophilic leukemia (RBL-2H3) mast cells and ...
more infohttps://scholars.okstate.edu/en/publications/alasup12supmcd-peptide-a-lead-peptide-to-inhibitors-of-immunoglob

Systemic mastocytosis - ONASystemic mastocytosis - ONA

Mast cell hyperactivity may mimic SM, but tissue accumulation of mast cells is not present and secondary organ dysfunction does ... The initial laboratory studies include serum tryptase and urinary histamine and PGD2 levels. An acute allergic reaction may ... Systemic mastocytosis with associated hematologic non-mast cell lineage disorder), and MCL (mast cell leukemia) have median ... Hypereosinophilia occurs with mutations in platelet-derived growth factor receptors (PDGF-R) which code for a response to ...
more infohttps://www.oncologynurseadvisor.com/hematology/systemic-mastocytosis/article/597056/

The Diagnosis and Management of Anaphylaxis
            
                Medical Education on ReachMDThe Diagnosis and Management of Anaphylaxis Medical Education on ReachMD

... not only systemic mast cell disease, but also some types of basophilic leukemia and acute myelocytic leukemia. So thats one ... where allergen binds to or actually bridges IgE molecules attached to high affinity receptors on the surface of mast cells and ... They can present with just dermatologic manifestations aside from when they have anaphylactic episode which are often salmon ... For example, patient who knows that they have had an allergic reaction to penicillin and is given penicillin, again for example ...
more infohttps://reachmd.com/programs/focus-on-allergy/the-diagnosis-and-management-of-anaphylaxis/3919/

Acute Urticaria: Background, Pathophysiology, EtiologyAcute Urticaria: Background, Pathophysiology, Etiology

... which are present on many cell types. The activation of the H1 histamine receptors on endothelial and smooth muscle cells leads ... is initiated by antigen-mediated IgE immune complexes that bind and cross-link Fc receptors on the surface of mast cells and ... Theoretically, almost any drug can cause an allergic reaction (see the images below); thus, allergic reactions to a wide ... Lymphoreticular malignancies (eg, chronic lymphocytic leukemia). * Pregnancy (ie, pruritic urticarial papules and plaques of ...
more infohttps://emedicine.medscape.com/article/137362-overview

Frontiers | Purinergic Signaling in Mast Cell Degranulation and Asthma | PharmacologyFrontiers | Purinergic Signaling in Mast Cell Degranulation and Asthma | Pharmacology

... originally thought that almost all allergic events were mediated directly only via the high-affinity immunoglobulin E receptors ... are also directly involved in mast cell degranulation, the release of inflammatory mediators such as histamine, serine ... as well as by ATP-gated P2X receptors, plays a significant role in mast cell degranulation. Both adenosine and ATP can induce ... as well as by ATP-gated P2X receptors, plays a significant role in mast cell degranulation. Both adenosine and ATP can induce ...
more infohttps://www.frontiersin.org/articles/10.3389/fphar.2017.00947/full

US8110591B2 - 2-aminoaryloxazole compounds as tyrosine kinase inhibitors 
        - Google PatentsUS8110591B2 - 2-aminoaryloxazole compounds as tyrosine kinase inhibitors - Google Patents

... allergic and degenerative disorders. More particularly, these compounds are potent and selective c-kit, bcr-abl, FGFR3 and/or ... mediated by certain native and/or mutant tyrosine kinases implicated in a variety of human and animal diseases such as cell ... The present invention relates to novel compounds selected from 2-aminoaryloxazoles that selectively modulate, regulate, and/or ... the category IV of mastocytosis includes the mast cell leukemia, characterized by the presence of circulating mast cells and ...
more infohttps://patents.google.com/patent/US8110591?oq=inassignee%3Adoubleclick

Dual Roles of IL-27 in Cancer Biology and ImmunotherapyDual Roles of IL-27 in Cancer Biology and Immunotherapy

... biological response modifiers and the family of cell adhesion-promoting molecules. ... IL-27Rα is present on T, B, and NK lymphocytes, neutrophils, monocytes, and mast cells, although it is found at lower levels in ... dendritic cells, and microglial cells, in response to stimuli acting through Toll-like receptors [65] or TNF-R-family members, ... T cell Th2 cytokine production, independently from Th1-related cytokines [93]. In addition, experimental allergic asthma was ...
more infohttps://www.hindawi.com/journals/mi/2017/3958069/

BLT2 Is Upregulated in Allergen-Stimulated Mast Cells and Mediates the Synthesis of Th2 Cytokines | The Journal of ImmunologyBLT2 Is Upregulated in Allergen-Stimulated Mast Cells and Mediates the Synthesis of Th2 Cytokines | The Journal of Immunology

In allergic reactions, cross-linking of the surface receptors for IgE on mast cells results in the synthesis of Th2 cytokines ... Peritoneal cell-derived mast cells: an in vitro model of mature serosal-type mouse mast cells. J. Immunol. 178: 6465-6475. ... In the present study, we found that Ag-stimulated bone marrow-derived mast cells (BMMCs) highly upregulated the expression of ... including histamine, proteases, arachidonic acid metabolites, and various cytokines (2). Th2 cytokines (e.g., IL-4 and IL-13) ...
more infohttp://www.jimmunol.org/content/185/10/6329?ijkey=555cb6f4562ebd40bd500cc4fbde183e52a70ff6&keytype2=tf_ipsecsha

Systemic mastocytosisSystemic mastocytosis

Mast cell hyperactivity may mimic SM, but tissue accumulation of mast cells is not present and secondary organ dysfunction does ... The initial laboratory studies include serum tryptase and urinary histamine and PGD2 levels. An acute allergic reaction may ... Systemic mastocytosis with associated hematologic non-mast cell lineage disorder), and MCL (mast cell leukemia) have median ... Hypereosinophilia occurs with mutations in platelet-derived growth factor receptors (PDGF-R) which code for a response to ...
more infohttps://www.thecardiologyadvisor.com/oncology/systemic-mastocytosis/article/619073/

Mechanisms of Hypersensitivity | Springer for Research & DevelopmentMechanisms of Hypersensitivity | Springer for Research & Development

Mediator release may occur via cross-linking of cell-bound IgE by... ... Allergic reactions to drugs are not always the result of the drugs protein-binding capacity, biotransformation, or degradation ... It is now known that mast cells express the H4 but not the H3 receptor, but exposure to histamine, or histamine in combination ... mast cells, and nasal polyps of allergic subject (see also Sects. 9.4.1, 9.4.3 and Fig. 9.3). IL-4-primed human mast cells ...
more infohttps://rd.springer.com/chapter/10.1007%2F978-1-4614-7261-2_3

Patent US7704506 - Fcε-PE chimeric protein for targeted treatment of allergy responses a method ... - Google PatentsPatent US7704506 - Fcε-PE chimeric protein for targeted treatment of allergy responses a method ... - Google Patents

... specifically and efficiently kills mouse mast cell lines expressing the FcεRI receptor, as well as primary mast cells derived ... The present invention also provides pharmaceutical compositions, for the treatment of allergic diseases and for the treatment ... The said chimeric protein is comprised of a cell targeting moiety for FcεRI expressing cells and a cell killing moiety. The ... The present invention provides a chimeric protein for targeted elimination of FcεRI expressing cells especially useful for the ...
more infohttp://www.google.com/patents/US7704506

Frontiers | Endocrine Factors Modulating Immune Responses in Pregnancy | ImmunologyFrontiers | Endocrine Factors Modulating Immune Responses in Pregnancy | Immunology

Furthermore, they are involved in the recruitment of mast cells and regulatory T cells into the fetal-maternal interface ... Furthermore, they are involved in the recruitment of mast cells and regulatory T cells into the fetal-maternal interface ... T and B cells. Pregnancy hormones also support the proliferation of pregnancy supporting uterine killer cells, retain ... T and B cells. Pregnancy hormones also support the proliferation of pregnancy supporting uterine killer cells, retain ...
more infohttps://www.frontiersin.org/articles/10.3389/fimmu.2014.00196/full

From IgE to Omalizumab | The Journal of ImmunologyFrom IgE to Omalizumab | The Journal of Immunology

Mast cells modulate allergic pulmonary eosinophilia in mice. Am. J. Respir. Cell Mol. Biol. 12: 404-409. ... IgE receptors, FcεRI, and CD23. Using rat basophilic leukemia cells, the binding properties of IgE and its FcεRI were ... Another known heterogeneity among IgE molecules is their reactivity to histamine-releasing factor (HRF). HRF can activate mast ... of membrane IgE on human IgE-switched B cells that is not present in serum IgE (108). FcεRI could also be targeted to ...
more infohttp://www.jimmunol.org/content/197/11/4187

World Allergy OrganizationWorld Allergy Organization

The growth , differentiation and survival of mast cells depends on stem cell factor (SCF) which binds KIT (CD117), a receptor ... Mast cell leukemia: This is the rarest and the most advanced form of SM. It is characterized by presence of ,20% immature mast ... Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions. Nature. 2015 Mar 12;519(7542):237- ... Mast Cell Mediators Related Symptoms:. Patients with cutaneous and systemic mastocytosis present with symptoms related to the ...
more infohttp://www.worldallergy.org/professional/allergic_diseases_center/mastocytosis/

Papular urticaria, urticaria papulosa | definition of papular urticaria, urticaria papulosa by Medical dictionaryPapular urticaria, urticaria papulosa | definition of papular urticaria, urticaria papulosa by Medical dictionary

Drugs that block histamine-1 (H1) receptors (antihistamines) are the primary treatment for urticaria. The use of both H1 and H2 ... The mast cell degranulation is due to an immunoglobulin E-mediated reaction to allergens (e.g., foods, drugs, or drug additives ... Urticaria is caused by vasodilation and increased permeability of capillaries of the skin due to the release by mast cells of ... with allergic reactions and histamine release or defects in the complement or kinin systems Risk factors Prior allergic ...
more infohttps://medical-dictionary.thefreedictionary.com/papular+urticaria%2C+urticaria+papulosa

Suppression of allergic and inflammatory responses by essential oils derived from herbal plants and citrus fruitsSuppression of allergic and inflammatory responses by essential oils derived from herbal plants and citrus fruits

Cells. RBL-2H3 rat basophilic leukemia and RAW264.7 murine macrophage cell lines were obtained from the American Type Culture ... Mast cell degranulation-mediated histamine release is accompanied by the release of β-hexosaminidase (13). Therefore, we ... Immunologically active mast cells and basophils express the high-affinity receptor for immunoglobulin E (IgE) on their surface ... In the present study, 20 EOs were selected as known herbal plant- and citrus fruit-derived EOs. Among the plant-derived EOs ...
more infohttps://www.spandidos-publications.com/ijmm/33/6/1643?text=fulltext

GENE RECOMBINANT ANTIBODY AND ANTIBODY FRAGMENT THEREOF - Patent applicationGENE RECOMBINANT ANTIBODY AND ANTIBODY FRAGMENT THEREOF - Patent application

... mast cell and the like as final inflammatory cells, such as a humanized IL-5 receptor antibody (WO 97/10354), a CC chemokine ... histamine by binding to an antigen immune complex with IgE produced from B cells to thereby induce an immediate allergic ... cancers including blood cancers such as leukemia. [0248] Examples of the present invention are shown below, but the present ... leukemia cells, particularly T cell leukemia cells, at a high frequency and reduces the number of CCR4-positive Leukemia cells ...
more infohttp://www.patentsencyclopedia.com/app/20120276090
  • The intense pruritus of urticaria is a result of histamine released into the dermis. (medscape.com)
  • urticaria pigmentosa, juvenile urticaria pigmentosa present at birth or in the first few weeks of life, usually disappearing before puberty, taking the form of a single nodule or tumor or of a disseminated eruption of yellowish brown to yellowish red macules, plaques, or bullae. (thefreedictionary.com)
  • However, conventional urticaria and angioedema are generally encountered in non-clonal, IgE mediated mast cell activation and are rare in clonal mast cell disorders such as mastocytosis. (thecardiologyadvisor.com)
  • Presence of these cardiovascular symptoms, particularly in the absence of urticaria and angioedema raises the index of suspicion for clonal mast cell disorders. (thecardiologyadvisor.com)
  • The intense pruritis of urticaria is a result of histamine released into the dermis. (oncologynurseadvisor.com)
  • ABBR: APUD cell Any of the constituent cells of a diffuse neuroendocrine system, having metabolic pathways that make and utilize serotonin (5-HT). (tabers.com)
  • These cells release histamine and serotonin that increase the permeability of the blood vessels and cause local oedema. (weebly.com)
  • PD-L1 expressed on tumor cells upon engagement with PD-1 at the cell surface of previously activated CTLs provides an inhibitory signal, which suppresses their functions and may induce apoptosis [ 9 ]. (hindawi.com)
  • As most tumors contain inflammatory cell infiltrates, which often include plentiful MCs, the question as to the possible contribution of MCs to tumor development has progressively been emerging. (springer.com)
  • In this chapter, the specific involvement of MCs in tumor biology and tumor fate will be considered, with particular emphasis on the capacity of these cells to stimulate tumor growth by promoting angiogenesis and lymphangiogenesis. (springer.com)
  • CD8+ cytotoxic T cells are able to kill virus-infected and tumor cells. (wikidoc.org)
  • Hyperplastic neuroretinopathy and disorders of pigment epithelial cells with a high incidence of subretinal tumor is present at 9 days ( Caffe et al 1993 ). (jax.org)
  • Isolated cells are then washed, counted using a hemocytometer and stained with fluorescent antibodies for subsequent analysis by flow cytometry. (jove.com)
  • This was shown in murine studies by using mast cell specific antibodies to separate them out immunomagnetically. (edu.au)
  • Those antibodies attach themselves to mast cells. (action-inter.net)
  • B cells make antibodies that bind to pathogens to enable their destruction. (wikidoc.org)
  • B cells not only make antibodies that bind to pathogens , but after an attack, some B cells will retain the ability to produce an antibody to serve as a 'memory' system. (wikidoc.org)
  • FcεRI), physical injury through pattern recognition receptors for damage-associated molecular patterns (DAMPs), microbial pathogens through pattern recognition receptors for pathogen-associated molecular patterns (PAMPs), and various compounds through their associated G-protein coupled receptors (e.g., morphine through opioid receptors) or ligand-gated ion channels. (wikipedia.org)
  • 2 More recent studies recognized additional, alternative, activating pathways including complement and signaling through microbial pattern recognition receptors, toll-like receptors (TLR). (asnjournals.org)
  • 8 The pattern recognition receptors, TLR, serve as an important link between innate and adaptive immunity. (asnjournals.org)
  • Previous studies have reported that both BLT1 and BLT2 are expressed in human and murine mast cells and also that both receptors contribute to the chemotactic migration of mast cells toward LTB 4 ( 12 , 23 ). (jimmunol.org)
  • Complement proteins can activate membrane receptors on mast cells to exert various functions as well. (wikipedia.org)
  • Histamine is the ligand for 2 membrane-bound receptors, the H1 and H2 receptors, which are present on many cell types. (medscape.com)
  • In this study, we show that receptor reorganization within the contacting mast cell membrane is markedly different upon binding of mobile and immobilized ligands. (jimmunol.org)
  • Receptors engaged with mobile ligand coalesce into large, cholesterol-rich clusters that occupy the central portion of the contacting membrane. (jimmunol.org)
  • Activated receptors are phosphorylated by Lyn, a membrane-tethered Src-family kinase, on tyrosine residues within ITAM domains of the FcεRI β and γ subunits. (jimmunol.org)
  • Multidrug resistance protein 4 (ABCC4) is a member of the family of ATP-binding cassette transporters required for the active transport of many bioactive substrates across the cell membrane . (thefreelibrary.com)
  • The membrane phospholipid phosphatidylinositol 4, 5-bisphosphate [PI(4,5)P 2 ] is a critical signal transducer in eukaryotic cells. (rupress.org)
  • Effective therapy often consists simply of antihistamines and mast cell membrane-stabilising compounds supplemented with medications targeted at specific symptoms and complications. (biomedcentral.com)
  • Close to two decades of research has established that astrocytes in situ and in vivo express numerous G protein-coupled receptors (GPCRs) that can be stimulated by neuronally-released transmitter. (jove.com)
  • We now report that exposure to IgE results in a striking (up to 32-fold) upregulation of surface expression of FcεRI on mouse mast cells in vitro or in vivo. (rupress.org)
  • The data from the present study demonstrated that RSV played a regulatory role in antagonizing the effects of IL‑33 on mast cells both in vitro and in vivo, suggesting that it has therapeutic potential in IL‑33‑mediated inflammatory diseases that are associated with mast cells. (spandidos-publications.com)
  • We hypothesize that, in vivo, mast cells protrude through epithelial cell barriers to provide distal sensors for relevant ligands. (biologists.org)
  • Structure of native agonists of the purinergic receptors, including both P2Rs (1-8) and ARs (9), and structures of agonist (10-20) and antagonist (21-37) ligands developed for the ARs, as described in the text. (frontiersin.org)
  • A mutein of the invention is a C5a receptor antagonist wherein the amino acid residue naturally occurring at sequence position 69 is mutated. (freepatentsonline.com)
  • Effects of a new C5a receptor antagonist on C5a- and endotoxin-induced neutropenia in the rat," (1999) Br. (freepatentsonline.com)
  • Transcriptional regulators AIRE and FEZ2 are expressed by the thymic medullary epithelium, and drives the transcription of organ-specific genes such as insulin to allow maturing thymocytes to be exposed to a more complex set of self-antigens than is present in the cortex. (wikipedia.org)
  • there are 25,400 microns in an inch), are arranged in patterns that contain antigens and cause IgE-receptors to cluster when the cells attach to the surface. (bio-medicine.org)
  • The alarming increase in the prevalence of allergic diseases over the past decade has led to a clear need for more effective treatment. (google.com)