Immobilization patterns and dynamics of acetate-utilizing methanogens immobilized in sterile granular sludge in upflow anaerobic sludge blanket reactors. (1/189)
Sterile granular sludge was inoculated with either Methanosarcina mazeii S-6, Methanosaeta concilii GP-6, or both species in acetate-fed upflow anaerobic sludge blanket (UASB) reactors to investigate the immobilization patterns and dynamics of aceticlastic methanogens in granular sludge. After several months of reactor operation, the methanogens were immobilized, either separately or together. The fastest immobilization was observed in the reactor containing M. mazeii S-6. The highest effluent concentration of acetate was observed in the reactor with only M. mazeii S-6 immobilized, while the lowest effluent concentration of acetate was observed in the reactor where both types of methanogens were immobilized together. No changes were observed in the kinetic parameters (Ks and mumax) of immobilized M. concilii GP-6 or M. mazeii S-6 compared with suspended cultures, indicating that immobilization does not affect the growth kinetics of these methanogens. An enzyme-linked immunosorbent assay using polyclonal antibodies against either M. concilii GP-6 or M. mazeii S-6 showed significant variations in the two methanogenic populations in the different reactors. Polyclonal antibodies were further used to study the spatial distribution of the two methanogens. M. concilii GP-6 was immobilized only on existing support material without any specific pattern. M. mazeii S-6, however, showed a different immobilization pattern: large clumps were formed when the concentration of acetate was high, but where the acetate concentration was low this strain was immobilized on support material as single cells or small clumps. The data clearly show that the two aceticlastic methanogens immobilize differently in UASB systems, depending on the conditions found throughout the UASB reactor. (+info)Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (2/189)
Viable cells of Pseudomonas aeruginosa were entrapped in alginate gel layers and incubated in a minimal glucose (15 g/L)-yeast extract (2 g/L)-salt medium to form artificial biofilm-like structures. After cultivation for 2 days, the biomass distribution inside the polymer was highly heterogeneous. The cell number reached approximately 1011 cells/g gel in the outer regions of the gel structures whereas the inner areas were less colonized (c. 10(8) cells g/gel). Killing of immobilized organisms by imipenem and tobramycin were compared with free-cell experiments (inoculum c. 10(9) cells/mL). Sessile-like bacteria displayed a higher resistance to the two antibiotics used alone or in combination than did suspended cells. Exposure for 10 h to 20 x MIC imipenem and 15 x MIC tobramycin reduced the number of viable immobilized bacteria to 0.3% and 3%, respectively, of the initial cell population, whereas these antibiotic concentrations were much more efficient (bactericidal) against free-cell cultures (5 log kill in 6 h). A synergic effect of tobramycin and imipenem was detected on bacterial suspensions but not on biofilm-like structures. Effective diffusivity measurements showed that the diffusion of imipenem in the alginate layer was not hindered. A slight but significant enhancement of beta-lactamase induction in immobilized cells as compared with their suspended counterparts was insufficient to explain the high resistance of sessile-like bacteria. (+info)Distribution of Nitrosomonas europaea and Paracoccus denitrificans immobilized in tubular polymeric gel for nitrogen removal. (3/189)
To improve the cooperative removal of nitrogen by Nitrosomonas europaea and Paracoccus denitrificans, we controlled their distribution in a tubular gel. When ethanol was supplied inside the tubular gel as an electron donor, their distributions overlapped in the external region of the gel. By changing the electron donor from ethanol to gaseous hydrogen, the distribution of P. denitrificans shifted to the inside of the tube and was separated from that of N. europaea. The separation resulted in an increase of the oxidation rate of ammonia by 25%. (+info)Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. (4/189)
Bifidobacterium longum KCTC 3128 and HLC 3742 were independently immobilized (entrapped) in calcium alginate beads containing 2, 3, and 4% sodium alginate. When the bifidobacteria entrapped in calcium alginate beads were exposed to simulated gastric juices and a bile salt solution, the death rate of the cells in the beads decreased proportionally with an increase in both the alginate gel concentration and bead size. The initial cell numbers in the beads affected the numbers of survivors after exposure to these solutions; however, the death rates of the viable cells were not affected. Accordingly, a mathematical model was formulated which expressed the influences of several parameters (gel concentration, bead size, and initial cell numbers) on the survival of entrapped bifidobacteria after sequential exposure to simulated gastric juices followed by a bile salt solution. The model proposed in this paper may be useful for estimating the survival of bifidobacteria in beads and establishing optimal entrapment conditions. (+info)Identification of a novel glycoprotein-binding activity in Streptococcus pyogenes regulated by the mga gene. (5/189)
The interaction between Streptococcus pyogenes and the host cell surface is not completely understood. Characterization of the adhesion mechanisms of the bacterium to the host cell surface is needed in order to develop new vaccines and anti-adhesion drugs. The presence of glycoprotein-binding activities among streptococcal strains was investigated. An activity binding to thyroglobulin, fetuin, asialofetuin and mucin but not non-glycosylated proteins was found to be present in the majority of the S. pyogenes strains studied. Cross-inhibition experiments suggested that the glycoproteins share a common structure recognized by the bacteria. The glycoprotein-binding activity was found to be proteinaceous, tightly attached to the bacterial surface and it also mediated the adherence of bacteria to solid surfaces coated with glycoproteins. The activity was found by transposon mutagenesis and complementation to be regulated by the multiple-gene regulator Mga, which has been implicated as a regulator of S. pyogenes virulence factors. (+info)Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface. (6/189)
Adhesion of Candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. C. albicans cells also adhere to several species of oral streptococci including Streptococcus gordonii, Streptococcus oralis and Streptococcus sanguinis in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of C. albicans cells by up to 2-3-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which C. albicans cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of S. gordonii cells but not to S. oralis cells. Parotid saliva that was pre-adsorbed with S. gordonii cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of C. albicans compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. C. albicans cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of C. albicans to S. gordonii cells. The results demonstrate that C. albicans cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote C. albicans adhesion and oral colonization. (+info)Response kinetics of tethered Rhodobacter sphaeroides to changes in light intensity. (7/189)
Rhodobacter sphaeroides can swim toward a wide range of attractants (a process known as taxis), propelled by a single rotating flagellum. The reversals of motor direction that cause tumbles in Eschericia coli taxis are replaced by brief motor stops, and taxis is controlled by a complex sensory system with multiple homologues of the E. coli sensory proteins. We tethered photosynthetically grown cells of R. sphaeroides by their flagella and measured the response of the flagellar motor to changes in light intensity. The unstimulated bias (probability of not being stopped) was significantly larger than the bias of tethered E. coli but similar to the probability of not tumbling in swimming E. coli. Otherwise, the step and impulse responses were the same as those of tethered E. coli to chemical attractants. This indicates that the single motor and multiple sensory signaling pathways in R. sphaeroides generate the same swimming response as several motors and a single pathway in E. coli, and that the response of the single motor is directly observable in the swimming pattern. Photo-responses were larger in the presence of cyanide or the uncoupler carbonyl cyanide 4-trifluoromethoxyphenylhydrazone (FCCP), consistent with the photo-response being detected via changes in the rate of electron transport. (+info)Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system. (8/189)
A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain GCH1 grew on propachlor with a generation time of 4.2 h and a rate of substrate utilization of 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but was subject to catabolite expression. Acetanilide was a growth substrate with a yield of 0.56 +/- 0.02 mg of protein micromol(-1). GCH1 strain cells were immobilized by adsorption onto a ceramic support and were used as biocatalysts in an immobilized cell system. Propachlor elimination reached 98%, with a retention time of 3 h and an initial organic load of 0.5 mM propachlor. The viability of immobilized cells increased 34-fold after 120 days of bioreactor operation. (+info)
Immobilized Cell Technology
... is a method of air filtration and purification that uses whole cell immobilization. It is a process ... Oxidation through immobilized cell technology is 12 times more efficient than natural oxidation is under the same conditions. ... As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that ... The key difference between traditional air filtration systems and those that employ immobilized cell technology is that the ...
Immobilized whole cell
... in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required ... The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is ... However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. Zaushitsyna, O.; ... Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience. This is only ...
Arthrobacter globiformis
1996). Immobilized Cells Basics and Applications. Burlington: Elsevier. ISBN 0-08-053447-3. Emanuel Goldman; Lorrence H. Green ... They may also become large, oval-shaped cells called cystite by growing them in very high carbon to nitrogen ratio environments ... These bacteria have cell walls that contain polysaccharides (with monomers glucose, galactose, and rhamnose), peptidoglycan, ... one of the few soluble osmotic regulators used by most cells. The complete genome of A. globiformis has been sequenced using ...
Succinyl coenzyme A synthetase
Since the cells do not have a functional citric acid cycle, acidosis results because cells are forced to choose lactic acid ... Wider de Xifra E, del C Batlle AM (Mar 1978). "Porphyrin biosynthesis: immobilized enzymes and ligands. VI. Studies on succinyl ... An interesting study using pigeon cells showed that GTP specific SCSs were located in pigeon liver cells, and ATP specific SCSs ... and it is located within the mitochondrial matrix of a cell. Succinyl CoA synthetase catalyzes the following reversible ...
Cell encapsulation
Yuet PK, Harris TJ, Goosen MF (1995). "Mathematical modelling of immobilized animal cell growth". Artif Cells Blood Substit ... autologous cells), from another donor (allogeneic cells) or from other species (xenogeneic cells). The use of autologous cells ... The cell type chosen for this technique depends on the desired application of the cell microcapsules. The cells put into the ... of extensive research is the use of stem cells such as mesenchymal stem cells for long term cell microencapsulation and cell ...
Biological photovoltaics
"A photoelectrochemical cell using immobilized photosynthetic membranes". Bioelectrochemistry and Bioenergetics. 22 (3): 391-401 ... Like other fuel cells, biological photovoltaic systems are divided into anodic and cathodic half-cells. Oxygenic photosynthetic ... The insulating outer membranes of whole cells impedes electron transfer from the sites of electron generation inside the cell ... and are sometimes also called photo-microbial fuel cells or "living solar cells". In a biological photovoltaic system, ...
Microbial fuel cell
"Biochemical cells utilizing immobilized cells of Clostridium butyricum". Biotechnology and Bioengineering. 19 (11): 1727-1733. ... Microbial fuel cell (MFC) is a type of bioelectrochemical fuel cell system that generates electric current by diverting ... Most microbial cells are electrochemically inactive. Electron transfer from microbial cells to the electrode is facilitated by ... The United States Navy is considering microbial fuel cells for environmental sensors. The use of microbial fuel cells to power ...
Arthrobacter citreus
Karigar, C; Mahesh, A; Nagenahalli, M; Yun, DJ (February 2006). "Phenol degradation by immobilized cells of Arthrobacter ... "Phenol degradation by immobilized cells of Arthrobacter citreus". Biodegradation. 17 (1): 47-55. doi:10.1007/s10532-005-3048-y ...
Bovine serum albumin
Zhang M, Desai T, Ferrari M (May 1998). "Proteins and cells on PEG immobilized silicon surfaces". Biomaterials. 19 (10): 953- ... BSA is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes ... BSA is also the main constituent of fetal bovine serum, a common cell culture medium. Acceptable daily intake Protein allergy ... During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often ...
Ribosome profiling
Lyse the cells or tissue and isolate the mRNA molecules bound to ribosomes. Immobilize complexes. This is commonly performed ... 2008). "A translational profiling approach for the molecular characterization of CNS cell types". Cell. 135 (4): 738-48. doi: ... This allows the starting codon of the mRNAs throughout the cell lysate to be analyzed, which has been used to determine non-AUG ... It produces a "global snapshot" of all the ribosomes actively translating in a cell at a particular moment, known as a ...
Luciferase
Biotinylation will immobilize luciferase on the cell-surface by binding to a streptavidin-biotin complex. This allows ... Different types of cells (e.g. bone marrow stem cells, T-cells) can be engineered to express a luciferase allowing their non- ... "Cell-surface-localized ATP detection with immobilized firefly luciferase". Analytical Biochemistry. 352 (1): 61-67. doi:10.1016 ... Huh S, Lee J, Jung E, Kim SC, Kang JI, Lee J, Kim YW, Sung YK, Kang HK, Park D (Jun 2009). "A cell-based system for screening ...
Biomolecular engineering
Examples of types of biomolecules that are immobilized are enzymes, organelles, and complete cells. Biomolecules can be ... Cellular engineering - Design of new cells using recombinant DNA and development of procedures to allow normal cells to adhere ... Attention is also given to the rudiments of engineered biomolecules in cell signaling, cell growth kinetics, biochemical ... Zhou, Quinn Z. K; Chen, Xiao Dong (2001). "Effects of temperature and pH on the catalytic activity of the immobilized β- ...
Milica Radisic
Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells ... Her work centers on using human embryonic and induced pluripotent stem cells to develop a heart patch that could be used to ... Radisic is known for creating beating heart tissue in a dish using human-induced pluripotent stem cells. Her research has led ... Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes SS Nunes, JW Miklas, J Liu, R Aschar- ...
VRLA battery
VRLA cells have the same chemistry, except the electrolyte is immobilized. In AGM this is accomplished with a fiberglass mat; ... When a cell discharges, the lead and diluted acid undergo a chemical reaction that produces lead sulfate and water. When a cell ... The gel cells were less likely to leak when the portable set was handled roughly. A modern gel battery is a VRLA battery with a ... The first AGM cell was the Cyclon, patented by Gates Rubber Corporation in 1972 and now produced by EnerSys. The cyclon is a ...
Barbatic acid
"Production of barbatic acid by immobilized cells of Cladonia miniata var. parvipes in calcium alginate". Journal of the Hattori ... Immobilised cells of the lichen Cladonia miniata var. parvipes have been used to synthesise barbatic acid. The cells were given ... Other research has shown that it is non-toxic to human peripheral blood mononuclear cells at concentrations that are effective ... It has cytotoxic and genotoxic activity against some tumour cell lines. And in vitro and in vivo experiments using various ...
Corynebacterium
"Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells". Biotechnology and ... It is an acute, contagious infection characterized by pseudomembranes of dead epithelial cells, white blood cells, red blood ... Keddie, R. M.; Cure, G. L. (1977). "The Cell Wall Composition and Distribution of Free Mycolic Acids in Named Strains of ... afermentans, C. auris, C. pseudodiphtheriticum, and C. propinquum). The cell wall is distinctive, with a predominance of ...
Albendazole
Cell motility, maintenance of cell shape, and intracellular transport are also disrupted. At higher concentrations, it disrupts ... Due to diminished ATP production, the parasite is immobilized and eventually dies. Some parasites have evolved some resistance ... drop in white blood cells, red blood cells, and platelets), hepatitis, acute liver failure, acute kidney injury, irreversible ... September 2012). "A cell-based screen reveals that the albendazole metabolite, albendazole sulfone, targets Wolbachia". PLOS ...
Cell signaling
"Capture of endothelial cells under flow using immobilized vascular endothelial growth factor". Biomaterials. 51: 303-312. doi: ... Cell biology/Pollard et al, The Cell/ G.M. Cooper Cell biology/Pollard et al, The Cell/ G.M. Cooper Cooper GM (2000). " ... In biology, cell signaling (cell signalling in British English) or cell communication is the ability of a cell to receive, ... Many cell signals are carried by molecules that are released by one cell and move to make contact with another cell. Signaling ...
CyTOF
Tagged antibodies may be in solution, conjugated to beads, or surface immobilized. The cell staining follows the same ... Spitzer MH, Nolan GP (May 2016). "Mass Cytometry: Single Cells, Many Features". Cell. 165 (4): 780-91. doi:10.1016/j.cell. ... cells can be probed with rhodium, an intercalator which can only penetrate dead cells. Then all cells are fixed and stained ... The cell introduction method of the mass cytometer is an aerosol splitter injection. The cells are then captured in a stream of ...
Titanate nanosheet
TiNSs may also be used for immobilizing biomolecules. When a monolayer of hemoglobin is intercalated into TiNSs, the electron ... Further, TiNSs potential as an electrocatalyst may enhance fuel cell efficiency during fuel oxidation. Similarly, intercalated ...
Biosensor
Cells tend to attach to the surface so they can be easily immobilized. Compared to organelles they remain active for longer ... A live-cell biosensor for cAMP can be used in non-lysed cells with the additional advantage of multiple reads to study the ... Advantages of tissues as biosensors include the following: easier to immobilize compared to cells and organelles the higher ... There is very high selectivity for transferrin receptors because they are over-expressed in cancer cells. If cells have high ...
Climacodon septentrionalis
Tanney, Joey B.; Hutchison, Leonard J. (2012-01-01). "The production of nematode-immobilizing secretory cells by Climacodon ... The mycelium of this species grows secretory cells the protrude outwards and develop branches that produce the substance in ... septentrionalis was the first observed species fungus not in the genus Agaricus to secrete a toxin that it uses to immobilize ...
Rotator cuff tear
Mesenchymal stem cells have no convincing evidence for their use overall, with quality human trials lacking. The greater ... First, the arm is immobilized so that the muscle can heal. Second, when appropriate, a therapist assists with passive exercises ... Pas HI, Moen MH, Haisma HJ, Winters M (July 2017). "No evidence for the use of stem cell therapy for tendon disorders: a ... These include injecting an individual's own stem cells, growth factors or platelet rich plasma (PRP) into the repair site, and ...
Laminaria
Nestle, Nikolaus F.E.I.; Kimich, Rainer (March 1996). "NMR Imaging of Heavy Metal Absorption in Alginate, Immobilized Cells, ... These structures are very similar to mesophyll cells found in higher plant leaves. A laminaria stick may be used to slowly ...
Arthrobacter rhombi
Elangovan, R.; Philip, Ligy; Chandraraj, K. (23 January 2009). "Hexavalent Chromium Reduction by Free and Immobilized Cell-free ...
Reactive bonding
In addition disposable microfluidic devices with sensing function and immobilized cells can be fabricated. Wafer bonding Direct ...
Cell-free protein array
Here the DNA was immobilized in the well together with an anti-GST antibody. Then cell-free expression mix was added and the ... Common sources of cell extracts used in cell-free protein array technology include wheat germ, Escherichia coli, and rabbit ... Cell extracts from other sources such as hyperthermophiles, hybridomas, Xenopus oocytes, insect, mammalian and human cells have ... Many of the advantages of cell-free protein array technology address the limitations of cell-based expression system used in ...
Ecological competence
Controlled release of immobilized cells as a strategy to regulate ecological competence of inocula. In Biotechnics/wastewater ( ... Increased plasmid stability leads to a higher ecological competence due to added spatial organization and regulated cell ...
Artificial cell
"Microcapsules prepared with different biomaterials to immobilize GDNF secreting 3T3 fibroblasts". International Journal of ... An artificial cell, synthetic cell or minimal cell is an engineered particle that mimics one or many functions of a biological ... The drop method is used for the creation of large artificial cells which encapsulate biological cells, stem cells and ... The most common method of preparation of artificial cells is through cell encapsulation. Encapsulated cells are typically ...
Kinetic-segregation model of T cell activation
Geppert, TD; Lipsky, PE (15 March 1987). "Accessory cell independent proliferation of human T4 cells stimulated by immobilized ... The TCR/peptide-MHC complex, formed when a T cell recognises its ligand on an antigen presenting cell (APC) and the T-cell-APC ... Immobilized superagonistic antibodies bound to CD28 exclude CD45 phosphatases completely and the signal leading to T-cell ... However, when these antibodies are immobilized (either by secondary antibody bound to plastic or by Fc receptors on other cells ...
Phage display
"CAR T Cells: Engineering Patients' Immune Cells to Treat Their Cancers". National Cancer Institute. 2013-12-06. Retrieved 9 ... By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein ... The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into E. coli bacterial cells such as ... These are made into synthetic receptors for T-Cells collected from the patient that are used to combat the disease. Competing ...
Substrate inhibition in bioreactors
... immobilizing the cells or increasing the biomass concentration. A fed-batch process is the most common way to decrease the ... The cell immobilization method the bioreactor works by encapsulating the cells into a material that makes the removal of ... With regards to bioreactor cell growth, substrate refers to the nutrients that the cells consume and is contained within the ... is the cell concentration, and P {\displaystyle P} is the product concentration. The product formation and cell growth are both ...
Extractive metallurgy
Electrometallurgy involves metallurgical processes that take place in some form of electrolytic cell. The most common types of ... "Electrochemical dissolution of chalcopyrite studied by voltammetry of immobilized microparticles". Chemical Papers. 60 (4): 293 ... and the valuable metal collects on the cathode of the cell. The fused salt electrolysis process is conducted at temperatures ... is continuously recovered in situ from the reduced iodide by electrochemical oxidation at the anode of an electrochemical cell ...
RBMX
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131. "Infection and Immunity Immunophenotyping ( ... "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...
Sprain
Protect: The injured site should be protected and immobilized, as there is an increased risk of recurrent injury to the ... Current Stem Cell Research & Therapy. 10 (1): 56-63. doi:10.2174/1574888x09666140710112535. PMID 25012736. Retrieved 2020-04-20 ... When a ligament is immobilized, the ligament has been shown to rapidly weaken. Normal daily activity is important for ... Rest: The joint affected should be immobilized and bearing weight should be minimized. For example, walking should be limited ...
Animal psychopathology
Tasty food can activate opioid receptors in the ventral tegmental area and thereby stimulate cells that release dopamine in the ... the dogs were immobilized using curare, a paralyzing drug while they were being shocked. Both the control group and group A ... Cortisol levels rise with age and hippocampal cells express fewer hormone receptors on their surface to protect themselves from ...
Joseph Katz (professor)
Cell Reports 20(11) (2017) 2612-2625. H. Ling, M. Fu, M. Hultmark, J. Katz, Effect of Reynolds Number and Saturation Level on ... A dinoflagellate exploits toxins to immobilize prey prior to ingestion, Proc. Natl. Acad. Sci. U. S. A. 107(5) (2010) 2082-2087 ...
Enzyme inhibitor
Mobley H (13 March 2006). "How do antibiotics kill bacterial cells but not human cells?". Scientific American. 294 (6): 98. ... in that they can be used to repel predators or competing organisms or immobilize prey. In addition, many drugs are small ... This causes the cell wall to lose strength and the bacteria to burst. In the figure, a molecule of penicillin (shown in a ball- ... MAIs have also been observed to be produced in cells by reactions of pro-drugs such as isoniazid or enzyme inhibitor ligands ( ...
TRIM63
... in order to immobilize it). All of these settings cause skeletal muscle atrophy. TRIM63/MuRF1 has been shown to interact with ... Journal of Cell Science. 115 (Pt 23): 4469-4482. doi:10.1242/jcs.00131. PMID 12414993. Kedar V, McDonough H, Arya R, Li HH, ... Cell Metabolism. 6 (5): 376-385. doi:10.1016/j.cmet.2007.09.009. PMID 17983583. McElhinny AS, Kakinuma K, Sorimachi H, Labeit S ... The Journal of Cell Biology. 157 (1): 125-136. doi:10.1083/jcb.200108089. PMC 2173255. PMID 11927605. Stitt TN, Drujan D, ...
Liver support system
... immortalized human cell lines and stem cells. The purpose of BAL-type devices, currently, is not to permanently replace liver ... "Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin ... Liver cells obtained from an animal were used instead of developing a piece of equipment for each function of the liver. The ... However, in the cell-seeded reactor there was an increase in the levels of ammonia, which the researchers stated could be ...
Industrial enzymes
Whole cells are typically used when a reaction requires a co-factor. Although co-factors may be generated in vitro, it is ... The complex is introduced into a support matrix for which the ligand has high binding affinity, and the enzyme is immobilized ... Typically, intracellular enzymes require cell lysis and separation of complex biochemical mixtures. Extracellular enzymes are ... Guisan, Jose (2006). Immobilization of Enzymes and Cells. Springer Science & Business Media. Jesionowski, Teofil; Zdarta, Jakub ...
Alkaline phosphatase
"Appendix E: Stem Cell Markers". Stem Cell Information. National Institutes of Health, U.S. Department of Health and Human ... Iqbal J (July 2011). "An enzyme immobilized microassay in capillary electrophoresis for characterization and inhibition studies ... One study compared the enzymes of liver metastases of giant-cell lung carcinoma and nonmalignant placental cells. The two were ... Leukocyte alkaline phosphatase is found within mature white blood cells. White blood cell levels of LAP can help in the ...
Single-walled carbon nanohorn
Sano, N; Ukita SI (2006). "One-step synthesis of Pt-supported carbon nanohorns for fuel cell electrode by arc plasma in liquid ... Tetracationic water-soluble porphyrin (H2P4+) could be immobilized by π-π stacking interactions onto the skeleton of SWNHs. The ... 2010). "A biofuel cell with a single-walled carbon nanohorn-based bioanode operating at physiological condition". Biosens. ... Also, carbon nanohorns possess strong catalytic property, which can be applied to fuel cell fabrication. Due to their ...
PIKFYVE
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... PIKfyve inhibitors cause cell death also in A-375 melanoma cells, which depend on autophagy for growth and proliferation, due ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. (Genes on human chromosome 2). ... "PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death". The Journal of Cell Biology. 187 (2): 279-94. doi: ...
TATA box
In specific cell types or on specific promoters TBP can be replaced by one of several TBP-related factors (TRF1 in Drosophila, ... The result is to immobilize the TATA-binding protein (TBP) on DNA in order to down-regulate transcription initiation. ... However, a change can be seen in HeLa cells with a TATAAAA to TATACAA which leads to a 20 fold decrease in transcription. Some ... CAAT boxes are rarely found in genes that express proteins ubiquitous in all cell types. The TATA box is a component of the ...
Epigenomics
A paradox exists, however, in that cells exhibit diverse responses to varying stimuli and that cells sharing identical sets of ... both immunoprecipitated DNA and non-immunoprecipitated onto a microarray containing immobilized gDNA. Analysis of the relative ... May 2007). "High-resolution profiling of histone methylations in the human genome". Cell. 129 (4): 823-37. doi:10.1016/j.cell. ... Kouzarides T (February 2007). "Chromatin modifications and their function". Cell. 128 (4): 693-705. doi:10.1016/j.cell.2007.02. ...
Reverse phase protein lysate microarray
Pellets from cells collected through any of the above means are lysed with a cell lysis buffer to obtain high protein ... AMAs immobilize antibodies that capture analytes from the sample applied on the microarray. The target protein is detected ... In addition, since RPMA can utilize whole-cell or undissected or microdissected cell lysates, it can provide direct ... Technically, minuscule amounts of (a) cellular lysates, from intact cells or laser capture microdissected cells, (b) body ...
Protocatechuic aldehyde
... of protocatechuic aldehyde and caffeic acid to vanillin and capsaicin in freely suspended and immobilized cell cultures of ... This molecule can be used as a precursor in the vanillin synthesis by biotransformation by cell cultures of Capsicum frutescens ...
Juliá-Colonna epoxidation
utilized the biphasic Juliá-Colonna epoxidation with immobilized poly-L-leucine (I-PLL) and urea hydrogen peroxide (UHP), and 8 ... have demonstrated cytotoxic activity against human tumor cells. Prilezhaev reaction Jørgensen epoxidation Asymmetric ... utilized the Juliá-Colonna epoxidation with PEG-immobilized poly-L-leucine (PEG-PLL) and DABCO hydrogen peroxide (DABCO-H2O2) ... Furthermore, in biphasic systems the polymer may be immobilized on polystyrene, polyethylene glycol (PEG), or silica gel and ...
List of Ig Nobel Prize winners
CD25+ cells". Journal of Cardiothoracic Surgery. 7: 26. doi:10.1186/1749-8090-7-26. PMC 3338095. PMID 22445281. "In Belarus, ... "The Pulmonary and Metabolic Effects of Suspension by the Feet Compared with Lateral Recumbency in Immobilized Black ... for electrically monitoring the activity of a brain cell in a locust while that locust was watching selected highlights from ... used as cryotherapy during high-dose melphalan conditioning reduces oral mucositis after autologous hematopoietic stem cell ...
Genous
The Genous Stent is a bio-engineered coronary stent coated with immobilized anti-CD34 monoclonal antibodies specific to the ... The pro-healing technology has an antibody surface coating that captures circulating CD34+ endothelial progenitor cells to the ... Genous is an endothelial progenitor cell (EPC) capture technology manufactured by OrbusNeich that promotes the accelerated ... endothelial progenitor cell-capturing stent system: a novel stent technology". Expert Review of Medical Devices. 6 (4): 365-375 ...
History of Sri Lanka (1948-present)
While developing secret cells and regional commands, Wijeweera's group also began to take a more public role during the ... Although their group was relatively small, the members hoped to immobilize the government by selective kidnapping and sudden, ...
ADA (buffer)
It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 ... and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors ...
Pepper spray
The Scoville rating of these sprays are 500,000 (sabre MK9 HVS unit) and 2,000,000 (Sabre, cell buster fog delivery). This was ... The definition under regulation states "any device designed to be used for the purpose of injuring, immobilizing or otherwise ... immobilizing or otherwise incapacitating any person" is a prohibited weapon. Only law enforcement officers may legally carry or ...
Ligand binding assay
... cells transfected with cloned receptors, and cells that are either in culture or isolated prior to analysis. Saturation binding ... The immobilized protein complex can be accomplished either in a single step or successively. IP can also be used in conjunction ... The device for which SPR is derived includes a sensor chip, a flow cell, a light source, a prism, and a fixed angle position ... In this type of assay the binding of a ligand to cells is followed over time. The obtained signal is proportional to the number ...
Ascariasis
White blood cell counts may demonstrate peripheral eosinophilia; this is common in many parasitic infections and is not ... which immobilizes the worm. It prevents migration when treatment is accomplished with weak drugs such as thiabendazole. If used ...
Phimosis
In this condition, the glans is swollen and painful, and the foreskin is immobilized by the swelling in a partially retracted ... Skin that is under tension expands by growing additional cells. There are different ways to stretch the phimosis. If the ...
Potential applications of graphene
Receptors on the cancer cell membrane bind TRAIL and cell surface enzymes clip the peptide thus release the drug onto the cell ... and carboxyl on its basal surface and edges that can be also used to immobilize or load various biomolecules for biomedical ... A graphene 'flying carpet' was demonstrated to deliver two anti-cancer drugs sequentially to the lung tumor cells (A549 cell) ... An advantage of graphene electrodes is the ability to produce inexpensive Schottky junction solar cells. Graphene solar cells ...
Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein...
Production of Peptides by Immobilized Growing Yeast Cells<...
1988). Production of Peptides by Immobilized Growing Yeast Cells. Annals of the New York Academy of Sciences, 542(1), 319-322. ... Production of Peptides by Immobilized Growing Yeast Cells. In: Annals of the New York Academy of Sciences. 1988 ; Vol. 542, No ... Production of Peptides by Immobilized Growing Yeast Cells. Annals of the New York Academy of Sciences. 1988 Dec;542(1):319-322 ... Production of Peptides by Immobilized Growing Yeast Cells. / TANAKA, ATSUO; SONOMOTO, KENJI; TAKASHIMA, YOSHIKI et al. In: ...
Immobilized microbial cells : based on a symposium jointly sponsored by the ACS Divisions of Microbial and Biochemical...
A high-throughput oxidative stress biosensor based on Escherichia coli rOGFP2 cells immobilized in a k-Carrageenan matrix<...
... we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a ... we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a ... we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a ... we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a ...
Super-resolution microscopy demystified | Nature Cell Biology
Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nat. Commun. 7, 13711 (2016). ... Nature Cell Biology (Nat Cell Biol) ISSN 1476-4679 (online) ISSN 1465-7392 (print) ... Sahl, S. J., Hell, S. W. & Jakobs, S. Fluorescence nanoscopy in cell biology. Nat. Rev. Mol. Cell Biol. 18, 685-701 (2017). ... Fernandez-Suarez, M. & Ting, A. Y. Fluorescent probes for super-resolution imaging in living cells. Nat. Rev. Mol. Cell Biol. 9 ...
ACROBiosystems Biotinylated Human NKG2A, Quantity: Each of 1
| Fisher Scientific
Nitrogen-Coordinated Single Cobalt Atom Catalysts for Oxygen Reduction in Proton Exchange Membrane Fuel Cells (Journal Article)...
Co/CoO nanoparticles immobilized on Co-N-doped carbon as trifunctional electrocatalysts for oxygen reduction, oxygen evolution ... PGM‐Free Cathode Catalysts for PEM Fuel Cells: A Mini‐Review on Stability Challenges journal, December 2018 * Shao, Yuyan; ... Carbon‐Based Metal‐Free ORR Electrocatalysts for Fuel Cells: Past, Present, and Future journal, January 2019 * Yang, Lijun; ... Iron-Based Catalysts with Improved Oxygen Reduction Activity in Polymer Electrolyte Fuel Cells journal, April 2009 * Lefèvre, ...
Tenascin-R Is an Intrinsic Autocrine Factor for Oligodendrocyte Differentiation and Promotes Cell Adhesion by a...
The fraction of brain polar GLs or individual polar GLs was immobilized in the wells of microtiter plates, and the binding of ... 1988) The L2/HNK-1 carbohydrate of neural cell adhesion molecules is involved in cell interactions. J Cell Biol 106:213-223. ... This procedure yielded a cell population containing a low number of GD3+O4−GFAP− OL precursor cells (∼15% of the total cell ... 1992) Cell death and control of cell survival in the oligodendrocyte lineage. Cell 70:31-46. ...
Protein protein interactions.pptx
Experimental Infection of Horses with West Nile virus - Volume 8, Number 4-April 2002 - Emerging Infectious Diseases journal -...
Briefly, 0.1 mL of sample was added to a monolayer of Vero cells in a six-well cell culture plate (Costar, Corning Incorporated ... Mosquitoes were immobilized by removing their legs and wings. Individual mosquito proboscises were placed in a capillary tube ... Virus titers ranged from 106.5 to 108.0 Vero cell PFU per mosquito and averaged 107.5,107.3, 107.3, 107.3, 107.6,107.3, 107.7, ... Cells were overlaid with 3 mL per well of 0.5% agarose in M-199 medium, supplemented with 350 mg/L of sodium bicarbonate, 29.2 ...
Diagnostic and Prognostic Value of Soluble Syndecan-1 in Pleural Malignancies
... but often with different functions and signalling properties than the cell-bound form. ,i,Aim,/i,. This study aimed to ... Syndecan-1 is a proteoglycan involved in various cellular functions and is cleaved from the cell surface in a regulated manner ... Cells were immobilized on SuperFrost Plus Slides (Thermo Fisher Scientific Inc., Waltham, MA, USA), using cytospin preparations ... Relation between Cell-Bound and Soluble Syndecan-1. To assess the relationship between cell-bound and soluble syndecan-1, we ...
Foamy Cells | The New York Academy of Sciences
Is the buildup of fatty molecules called phospholipids inside cells a harmless side effect or a sign of toxicity? And what does ... Rapid-gradient HPLC method for measuring drug interactions with immobilized artificial membrane: comparison with other ... A cell-based approach for the early assessment of the phospholipidogenic potential in pharmaceutical research and drug ... The occurrence of huge cells in pulmonary alveoli of rats treated by an anorexic drug. Arzneimittelforschung. 20: 417.. Kacew, ...
Sustainability | Free Full-Text | Performance Prediction of Proton Exchange Membrane Fuel Cells (PEMFC) Using Adaptive Neuro...
The training and testing of the model were then utilized in the prediction of the cell-characteristic performance. The model ... and this stipulates that ANFIS can predict characteristic behavior of fuel cell performance with very high accuracy. ... In-situ synthesis of Ni/N-doped CNFs-supported graphite disk as effective immobilized catalyst for methanol electrooxidation. ... Fuel Cell Testing Procedure. Fuel cells obtained from fuel cell store United States with an active area of 11.46 cm2 was used ...
Project A06 - Improving intravital microscopy of inflammatory cell response by active motion compensation with controlled...
Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nat Commun 2016;7: 13711. Abstract Muller M, ... Cell-to-cell transfer of HIV-1 via virological synapses leads to endosomal virion maturation that activates viral membrane ... Cell Host Microbe 2011;10: 551-562. Abstract Rumpf M, Wirth B. An Elasticity-Based Covariance Analysis of Shapes. Int J Comput ... Cell Rep 2017;18: 1256-1269. Abstract. 2016. Absil P-A, Gousenbourger P-Y, Striewski P, Wirth B. Differentiable Piecewise- ...
Frontiers | Interaction of the Factor H Family Proteins FHR-1 and FHR-5 With DNA and Dead Cells: Implications for the...
FHR-1 and FHR-5 bound to late apoptotic and necrotic cells and recruited monomeric C-reactive protein and pentraxin 3, and vice ... FHR-1 and FHR-5 bound to late apoptotic and necrotic cells, and recruited monomeric C-reactive protein and pentraxin 3, and ... Factor H (FH), a major soluble complement inhibitor, binds to dead cells and inhibits excessive complement activation on their ... Factor H (FH), a major soluble complement inhibitor, binds to dead cells and inhibits excessive complement activation on their ...
AA FG battery
Journal name: World journal of microbiology & biotechnology - PubAg Search Results
bioaccumulation; immobilized cells. Abstract:. ... Thirty species of microorganisms (8 bacteria, 9 actinomycetes, 8 fungi and 5 ... acid value; beers; brewers yeast; cell wall components; cell walls; decanoic acid; fermentation; flavor; freshness; genes; ... Escherichia coli; adhesion; bacteria; blood flow; cell culture; chickens; epithelial cells; epithelium; models; respiratory ... 8-hydroxyquinoline; Streptomyces; apoptosis; biotechnology; caspase-3; caspase-8; cell proliferation; cell viability; ...
Tranmission Electron Microscopy with a Liquid Flow Cell | NIST
Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized ... such as an impermeable cell with electron transparent windows. Recently, a liquid flow cell TEM holder equipped with a ... Klein, K. , Anderson, I. and de, N. (2011), Tranmission Electron Microscopy with a Liquid Flow Cell, Journal of Microscopy, [ ...
Sarah Sherman, Ph.D. - Senior Associate - Biomechanics | Exponent
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells | STEMCELL Technologies
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional ... During semi-solid cloning, individual cells are immobilized within a semi-solid medium and grow into discrete, monoclonal ... Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells Cloning hybridomas and CHO cells can be done faster and more ... Human Primary Cells. It All Starts With the Right Cells View Products , ...
Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory | eLife
Resistance to 7M Urea is measuring the avidity of a divalent binding of antibody to immobilized target. The competition assay ... Similar memory T-cell stimulation by variant dengue E-proteins. Memory T-cells are necessary for memory B-cell responses ... When a virus enters the body, a group of immune cells known as B cells gets activated. Certain B cells can recognise the ... the proportion of IgM+ GC B-cells fell over time (Figure 4C) and levels of VH mutation in all B-cells and IgM+ B cells ...
Human/Mouse Lipoprotein Lipase/LPL Antibody AF7197: R&D Systems
Once secreted, the circulating enzyme ultimately becomes immobilized on the surface of endothelium by binding to cell surface ... Journal of Cell Science, 2021;0(0):.. Species: Mouse. Sample Types: Cell Lysates. Applications: Western Blot ... It is produced by multiple cell types, including adipocytes, skelelal muscle cells and macrophages. ... Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF ...
The state of carrying a developing embryo or fetus within the uterus. Medically, someone is considered to be pregnant when an...
If cell development continues and a blastocyst implants in the uterus, it will become an embryo and create a pregnancy. ... A chemical agent used for birth control which works by immobilizing or killing sperm. ... The initial cell formed when a sperm has fertilized an ovum, which then travels down the fallopian tube for a few days, ... A reproductive cell, sometimes also called an egg. Ova (plural) are stored in the ovaries and released into the fallopian tubes ...
Biotin Anti-Mouse CD3e, 145-2C11 | SouthernBiotech
... is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen ... receptor (TCR). It is expressed on all T cells of all mous ... a member of the immunoglobulin superfamily of cell surface ... Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell ... In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145- ...
Flashcards - Medical Micro Exam 3
Penetrates endothelial cells lining small blood vessels*Multiplication in endothelial cells*Cell rupture*Leakage thrombosis and ... Immobilized T. pallidum , patient serum , fluorescently tagged anti-human Ab.* Treponema pallidum particle agglutination (TP-PA ... Kills all cells in path - creates more necrosis *If untreated - bacteremia & death* ... False membrane of fibrin, mucus and inflammatory cells line intestinal mucosa *-White plaques*-Profuse diarrhea, abdominal ...
Mapping of Structure-Function Peptide Sites on the Human Alpha-fetoprotein Amino Acid Sequence
... defined to include essentially all secreted molecules that are immobilized in the matrix outside of cells. The functions of ... The cell adhesion molecules (CAMs) mediate cell-cell and ECM interactions during embryonic development, maintenance of adult ... it might inhibit cell migration (spreading), cell adhesion, and cell proliferation. In other words, AFP may be a partial ... cell junction formation, and polarity by targeting cell-surface adhesion to specific ECM proteins/ligands on adjacent cells. ...
AntibodiesNanoparticlesBiosensorProteinReactiveAntigenProteinsBiolSickle cell diEndothelial cellsDetectionInteractionBiologyMembranePeptidesStimulationOxidativeAttachmentAntigensReceptorProliferation2000ComplementingBacterialReceptorsGastrointestinalImmuneSuitableBehaviorMechanismRecognizeEnablesMolecularActivationProcessHighMechanismsAdditionGrowthPatientsDataEffectsCultureTypeLineResponsesBodyEntryFreeSingle
Antibodies7
- Recent research in the lab has shown that viral ability to recognize and invade host cells in the presence of immune system antibodies is determined by the viral particle structure. (nih.gov)
- Effects of stimulation with soluble or immobilized CD3 antibodies. (nih.gov)
- Another contraceptive strategy used antibodies to cause sperm to clump together and become immobilized, a process called agglutination. (nih.gov)
- Recombinant antibodies from GC B-cells showed a higher affinity for the variant antigen than antibodies from a primary response, confirming a memory origin. (elifesciences.org)
- Certain B cells can recognise the invader and produce specific proteins, the antibodies, which can target and kill the invader. (elifesciences.org)
- It is an endothelial progenitor cell (EPC) capture stent that was developed with immobilized antibodies targeted at EPC surface antigens with the aim of rapidly forming an endothelial layer that secretes abluminal cytokines, which may reduce smooth muscle cell growth and intimal hyperplasia. (medscape.com)
- The Immobilized Protein A 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. (gbiosciences.com)
Nanoparticles6
- Fabrication of water-dispersible and cell-stimulating calcium phosphate nanoparticles immobilizing basic fibroblast growth factor. (bvsalud.org)
- Biocompatible calcium phosphate (CaP) nanoparticles (NPs) co-immobilizing bFGF, heparin , and ferucarbotran would be useful as a multifunctional delivery carrier of bFGF. (bvsalud.org)
- Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. (nist.gov)
- Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness. (nist.gov)
- Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. (beilstein-journals.org)
- Selectivity can be established using immobilized biomolecules on nanoparticles for interaction with proteins, peptides and nucleic acid sequences. (utoronto.ca)
Biosensor3
- Our aim is to fabricate a sensitive whole-cell oxidative stress biosensor which has improved properties that address the mentioned weaknesses. (elsevierpure.com)
- In this paper, we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a k-carrageenan matrix, for the detection of oxidative stress challenged by metalloid compounds. (elsevierpure.com)
- Ooi, L, Heng, LY & Mori, IC 2015, ' A high-throughput oxidative stress biosensor based on Escherichia coli rOGFP2 cells immobilized in a k-Carrageenan matrix ', Sensors (Switzerland) , vol. 15, no. 2, pp. 2354-2368. (elsevierpure.com)
Protein15
- Recombinant Protein;20UG;ActiveMax Human IL-4, Tag Free (IL4-H4218) is expressed from human 293 cells (HEK293). (fishersci.com)
- Complementing our research on virus cell entry is our effort to define the mechanisms of virus particle assembly and regulation of the assembly outcomes (i.e., structural features of virus particles such as shape, size, or viral protein incorporation). (nih.gov)
- 1. pim-1 proto-oncogene expression in anti-CD3-mediated T cell activation is associated with protein kinase C activation and is independent of Raf-1. (nih.gov)
- 8. Age-related reductions in the activation of mitogen-activated protein kinases p44mapk/ERK1 and p42mapk/ERK2 in human T cells stimulated via ligation of the T cell receptor complex. (nih.gov)
- 11. The role of protein kinase C in transmembrane signaling by the T cell antigen receptor complex. (nih.gov)
- 14. Induction of glucose-regulated protein 78 by chronic hypoxia in human gastric tumor cells through a protein kinase C-epsilon/ERK/AP-1 signaling cascade. (nih.gov)
- The active when overexpressed from a multicopy plasmid, DinI form of RecA is a protein filament formed on ssDNA in conferred significant UV sensitivity to cells and pre- an ATP-dependent fashion. (nih.gov)
- Variant protein boosts induced GCs with a higher proportion of IgM+ B cells. (elifesciences.org)
- The most variant protein re-stimulated GCs with the highest proportion of IgM+ cells with the most diverse, least mutated V-genes and with a slower but efficient serum antibody response. (elifesciences.org)
- Without the original protein injection, the response without the immature memory B cells was not as efficient. (elifesciences.org)
- The body therefore has a pool of memory B cells that can recognise a wider range of virus protein variants than the ones that caused the first immune reaction. (elifesciences.org)
- Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). (gbiosciences.com)
- Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. (gbiosciences.com)
- Immobilized Human IgG is affinity resin used to purify Protein A and Protein A tagged recombinant proteins and is suitable for tandem affinity purific. (gbiosciences.com)
- In this study, we show in human and frog cells that Plk4 interacts with the centrosome protein Cep152, the orthologue of Drosophila melanogaster Asterless. (rupress.org)
Reactive3
- This reveals a new process of antibody memory, that IgM memory cells with fewer mutations participate in secondary responses to variant antigens, demonstrating how the hierarchical structure of B-cell memory is used and indicating the potential and limits of cross-reactive antibody based immunity. (elifesciences.org)
- Mechanistically, we highlight the significance of immobilized peroxidase reactive intermediates vs diffusible small molecule oxidants (hypochlorous and hypobromous acids) for the overall oxidative biodegradation process in neutrophils and eosinophils. (cdc.gov)
- The activation of XO and reduced Tetrahydrobiopterin (BH 4 ) levels in endothelial cells leads to increased reactive oxygen species (ROS) formation, which in turn leads to endothelial nitric oxide synthase (eNOS) uncoupling and further production of superoxide (Thomas, et al 2010). (justia.com)
Antigen2
- 12. Regulation of gamma T-cell antigen receptor expression by intracellular calcium in acute lymphoblastic leukemia cell line DND41. (nih.gov)
- Lipoprotein Lipase/LPL was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
Proteins4
- Compared to the fully mature cells, the immature memory B cells were much better at recognizing the variants of the proteins, and these cells then multiplied and mounted an immune response. (elifesciences.org)
- The multiple molecular variant forms of AFP are discussed in relation to published reports of AFP binding proteins and cell surface receptors. (atlasgeneticsoncology.org)
- Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (who.int)
- The Sickled-shaped erythrocytes together with endothelial cells, activated leukocytes, platelets and plasma proteins participate in the multistep vaso-occlusion process (Frenette 2002). (justia.com)
Biol2
- Cell Biol. (nature.com)
- J Cell Biol (2010) 191 (4): 721-729. (rupress.org)
Sickle cell di6
- Dr. Keith Hoots, the Division Director at the Division of Blood Diseases and Resources (DBDR) opened the meeting by greeting the Sickle Cell Disease Advisory committee (SCDAC) members. (nih.gov)
- In 1999 there were 2 major obstacles for curing adults with sickle cell disease. (nih.gov)
- The present invention includes embodiments for treatment and/or prevention of sickle cell disease that employ Hydroxyfasudil or Isocoronarin D alone or either in conjunction with each other or an inducer of HbF production. (justia.com)
- In particular aspects, the field of the present invention includes treatment and/or prevention of a blood disorder, such as sickle cell disease. (justia.com)
- Sickle cell disease (SCD) is the most common life-threatening monogenic disorder in the world with statistics indicating that approximately 80% (230,000) of children affected globally are born in sub-Saharan Africa (Modell and Darlison 2008). (justia.com)
- Sickle cell disease (SCD) can arise from a single point mutation that causes erythrocyte deformation or sickle-shaped erythrocytes (Ingram 1957). (justia.com)
Endothelial cells1
- A coating strategy to enhance endothelial restoration by utilizing 'pro-healing' substances not eluted by the stent, but which attract endothelial cells, may reduce the thrombogenicity of the device. (medscape.com)
Detection1
- Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1. (boku.ac.at)
Interaction3
- 18. Interaction of CD4:lck with the T cell receptor/CD3 complex induces early signaling events in the absence of CD45 tyrosine phosphatase. (nih.gov)
- When the suramin-conjugated beads were added to the cells on cell culture inserts, which preclude an interaction with the cell surface but allow interaction with the culture medium, there was no effect on proliferation. (nih.gov)
- Suramin need not enter NCI-H596 cells to exert its growth-stimulatory effect, nor is this effect mediated by an interaction with soluble growth factors. (nih.gov)
Biology2
- We combine molecular virology, in vitro reconstitution, biophysics, and quantitative cell biology to answer difficult-to-tackle mechanistic questions in virology and build upon those discoveries to ultimately enable prediction and prevention of undesirable viral adaptation (e.g., drug resistance, immune escape, or pandemic adaptation). (nih.gov)
- The field of the present invention includes at least biology, cell biology, and medicine. (justia.com)
Membrane1
- Viruses initiate infection by delivering the genomes from within the viral capsid or a membrane envelope to the target cell. (nih.gov)
Peptides1
- We further observed that the addition of cell-penetrating peptides, Tat and T7, did not increase BBB penetration above the aptamer-loaded LNPs alone. (beilstein-journals.org)
Stimulation2
Oxidative2
- Here, we review the role and contribution of different oxidative enzymes of inflammatory cells - myeloperoxidase, eosinophil peroxidase, lactoperoxidase, hemoglobin, and xanthine oxidase - to the reactions of nanoparticle biodegradation. (cdc.gov)
- We consider possible involvement of oxidative machinery of other professional phagocytes such as microglial cells, myeloid-derived suppressor cells, in the context of biodegradation relevant to targeted drug delivery. (cdc.gov)
Attachment1
- Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. (nih.gov)
Antigens1
- Vaccines induce memory B-cells that provide high affinity secondary antibody responses to identical antigens. (elifesciences.org)
Receptor1
- 10. Activation of nuclear factor-kappaB via T cell receptor requires a Raf kinase and Ca2+ influx. (nih.gov)
Proliferation3
- No. IL4-H4218) stimulates the proliferation of TF-1 human erythroleukemic cells. (fishersci.com)
- The NPs promoted proliferation of baby hamster kidney fibroblasts (BHK-21 cells ) and mouse osteoblastic MC3T3-E1 cells at a certain dose range, although they inhibited proliferation of rat pheochromocytoma (PC-12) cells . (bvsalud.org)
- These results demonstrated that the effect of the NPs on cell proliferation was dependent on the cell type and dose , the details of which should be investigated in a future study. (bvsalud.org)
20002
- In an effort to target LNPs composed of an ionizable cationic lipid (DLin-MC3-DMA), cholesterol, the phospholipid 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), and 1,2-dimyristoyl- rac -glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG 2000) to particular cell types, as well as to generate LNPs that can cross the BBB, we developed and assessed two approaches. (beilstein-journals.org)
- 2000. N-glycans influence adhesive and invasive behavior in vitro of three metastatic cell. (bchi.lt)
Complementing1
- Complementing the microfluidic cell culture work, surface enhanced Raman spectroscopy (SERS), a label-free vibrational spectroscopic technique that lends itself well to use in aqueous systems, was used to detect bacterial signaling molecules. (umd.edu)
Bacterial2
- This research is expected to provide a new direction in performing high-throughput environmental toxicity screening with living bacterial cells which is capable of measuring the bioavailability and toxicity of environmental stressors in a friction of a second. (elsevierpure.com)
- When a bacterial cell is subjected to the harmful action leading to the termination of the SOS response. (nih.gov)
Receptors1
- We are further working to define how the structural organization of virus particles might enable entry into cells not displaying fitting receptors. (nih.gov)
Gastrointestinal2
- A major factor in alcohol-related disease stems from the ability of alcohol to compromise the natural barrier function of cells in the gastrointestinal tract, increasing the movement of toxins from the intestines to other organs in the body. (nih.gov)
- Such AFP peptide segments could potentially serve as delivery agents which could target vascular, neuroendocrine, or gastrointestinal cells. (atlasgeneticsoncology.org)
Immune3
- When a virus enters the body, a group of immune cells known as B cells gets activated. (elifesciences.org)
- If the same virus enters the organism again, the memory B cells rapidly identify it and produce a quicker and more efficient immune response than during the first attack. (elifesciences.org)
- As a former astronaut, Hughes-Fulford aims to apply lessons learned from studies of immune cells in microgravity to a new model for investigating the loss of immune response in older women and men. (nih.gov)
Suitable1
- Biosensors fabricated with whole-cell bacteria appear to be suitable for detecting bioavailability and toxicity effects of the chemical(s) of concern, but they are usually reported to have drawbacks like long response times (ranging from hours to days), narrow dynamic range and instability during long term storage. (elsevierpure.com)
Behavior1
- The model exhibited a perfect correlation between the predicted and experimental data, and this stipulates that ANFIS can predict characteristic behavior of fuel cell performance with very high accuracy. (mdpi.com)
Mechanism2
- Apoptosis as a mechanism for myonuclear loss with atrophy is controversial, whereas cell death of satellite cells has not been considered. (frontiersin.org)
- This dissertation describes the development of a microfluidic system for immobilizing and culturing of cells that also enables control over the genetic composition of the bacteria and their subsequent response to environmental stimuli via a new nonviral nucleic acid delivery mechanism. (umd.edu)
Recognize4
- Viral outbreaks and pandemics arise when animal viruses adapt so that they can recognize and use human cells as their host. (nih.gov)
- During the infection some of these B cells become 'memory B cells', having gone through a maturation process that hones their ability to specifically recognize this particular microbe. (elifesciences.org)
- However memory B cells may not be able to recognize a previous intruder if it has changed too much. (elifesciences.org)
- Some cells are fully mature and can quickly recognize the original virus. (elifesciences.org)
Enables1
- This insight led to a model where the pre-existing diversity in virus particle size in pleomorphic (mixed-shape) viruses enables viral adaptation to cell-entry pressure in a way that does not require an initiating genetic change. (nih.gov)
Molecular2
- In their pursuit of understanding cellular function, biologists seek to observe the processes that allow cells to maintain homeostasis and react dynamically to internal and external cues-on both a molecular scale and inside structurally intact, ideally living specimens. (nature.com)
- Molecular signaling pathways activated in muscle fibers by disuse are rarely considered within satellite cells themselves despite similar exposure to unloading or low mechanical load. (frontiersin.org)
Activation1
- 20. CD4 ligands inhibit the formation of multifunctional transduction complexes involved in T cell activation. (nih.gov)
Process3
- But others have not finished their maturation process: these cells are less focussed, and cannot target the original microbe with the same exact precision shown by mature memory cells. (elifesciences.org)
- These cells play a key role in bone remodeling, a process that is vital to skeletal health. (nih.gov)
- Endothelial progenitor cells have also been identified as a key factor in the re-endothelialization process after stent implantation. (medscape.com)
High3
- Influenza cell-entry glycoprotein hemagglutinin (HA) is present at a high density on virus particles. (nih.gov)
- Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. (nist.gov)
- Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept. (boku.ac.at)
Mechanisms1
- The purpose of this study was to examine the mechanisms by which suramin exerts this growth-stimulatory effect in NSCLC cells. (nih.gov)
Addition1
- NCI-H596 cells were treated with agarose-immobilized suramin, directly or by addition on cell culture inserts, after which growth was determined by [3H]thymidine incorporation. (nih.gov)
Growth3
- Satellite cells are particularly responsive to myostatin and to growth factors. (frontiersin.org)
- We have reported that suramin, at dose concentrations higher than 140 microM, exerts growth-stimulatory effects in several non-small-cell lung cancer (NSCLC) cell lines. (nih.gov)
- Agarose-immobilized suramin stimulated NCI-H596 cell growth, but only when added directly to the cells. (nih.gov)
Patients1
- Paola Divieti, M.D., Ph.D., Massachusetts General Hospital/Harvard Medical School, Boston: Weight-bearing activities contribute to the development and maintenance of bone mass, while weightlessness and immobility - as experienced by the astronauts and bedridden and immobilized patients - can result in bone loss and a weakened skeleton. (nih.gov)
Data1
- Blank cells indicate that no data were available in the literature. (nih.gov)
Effects1
- Electrophysiological studies in mammalian cells transfected with human K v 7.2 and/or K v 7.3 cDNAs revealed that each of these four mutations stabilized the activated state of the channel, thereby producing gain-of-function effects, which are opposite to the loss-of-function effects produced by previously found mutations. (jneurosci.org)
Culture1
- Using microgravity three-dimensional cell culture models, McCole plans to generate insights regarding the barrier properties of the intestines, and to explore how the absence of gravity affects alcohol's ability to diminish this barrier. (nih.gov)
Type2
- Osteocytes, the most common type of bone cell, are believed to have gravity-sensing abilities. (nih.gov)
- The most common type of SCD is sickle cell anemia (SCA) (also referred to as HbSS or SS disease or hemoglobin S) in which there is homozygosity for the mutation that causes HbS. (justia.com)
Line3
- Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. (rndsystems.com)
- Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. (rndsystems.com)
- Lipoprotein Lipase/LPL in SH‑SY5Y Human Cell Line. (rndsystems.com)
Responses1
- Skeletal muscle stem cells (satellite cells) and myonuclei are integrally involved in skeletal muscle responses to environmental changes that induce atrophy. (frontiersin.org)
Body2
- Osteoclasts are the only cells in the body that have a bone-destroying capability. (hindawi.com)
- Mucus trapping can prevent pathogens from infecting cells and accelerate their removal from the body. (nih.gov)
Entry1
- We report herein a CCR5-selective RNA aptamer that acts to facilitate entry through a simplified BBB model and that drives the uptake of LNPs into CCR5-expressing cells, while the gp160 aptamer did not. (beilstein-journals.org)
Free1
- Immobilized ActiveMax Human IL-4, Tag Free (Cat. (fishersci.com)
Single1
- Real- time measurements on the spatial and temporal fluctuations of single molecules in living cells, which are not possible using other methods, are a major goal of this initiative. (nih.gov)