Immobilized Cell Technology

Immobilized whole cell

Arthrobacter globiformis

Succinyl coenzyme A synthetase

Cell encapsulation

Biological photovoltaics

Microbial fuel cell

Arthrobacter citreus

Bovine serum albumin

Ribosome profiling

Luciferase

Biomolecular engineering

Milica Radisic

VRLA battery

Barbatic acid

Corynebacterium

Albendazole

Cell signaling

CyTOF

Titanate nanosheet

Biosensor

Climacodon septentrionalis

Rotator cuff tear

Laminaria

Arthrobacter rhombi

Reactive bonding

Cell-free protein array

Ecological competence

Artificial cell

Kinetic-segregation model of T cell activation

Phage display

Substrate inhibition in bioreactors

Extractive metallurgy

RBMX

Sprain

Animal psychopathology

Joseph Katz (professor)

Enzyme inhibitor

TRIM63

Liver support system

Industrial enzymes

Alkaline phosphatase

Single-walled carbon nanohorn

PIKFYVE

TATA box

Epigenomics

Reverse phase protein lysate microarray

Protocatechuic aldehyde

Juliá-Colonna epoxidation

List of Ig Nobel Prize winners

Genous

History of Sri Lanka (1948-present)

ADA (buffer)

Pepper spray

Ligand binding assay

Ascariasis

Phimosis

Potential applications of graphene

Antibodies7

• Recent research in the lab has shown that viral ability to recognize and invade host cells in the presence of immune system antibodies is determined by the viral particle structure. (nih.gov)
• Effects of stimulation with soluble or immobilized CD3 antibodies. (nih.gov)
• Another contraceptive strategy used antibodies to cause sperm to clump together and become immobilized, a process called agglutination. (nih.gov)
• Recombinant antibodies from GC B-cells showed a higher affinity for the variant antigen than antibodies from a primary response, confirming a memory origin. (elifesciences.org)
• Certain B cells can recognise the invader and produce specific proteins, the antibodies, which can target and kill the invader. (elifesciences.org)
• It is an endothelial progenitor cell (EPC) capture stent that was developed with immobilized antibodies targeted at EPC surface antigens with the aim of rapidly forming an endothelial layer that secretes abluminal cytokines, which may reduce smooth muscle cell growth and intimal hyperplasia. (medscape.com)
• The Immobilized Protein A 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. (gbiosciences.com)

Nanoparticles6

• Fabrication of water-dispersible and cell-stimulating calcium phosphate nanoparticles immobilizing basic fibroblast growth factor. (bvsalud.org)
• Biocompatible calcium phosphate (CaP) nanoparticles (NPs) co-immobilizing bFGF, heparin , and ferucarbotran would be useful as a multifunctional delivery carrier of bFGF. (bvsalud.org)
• Recently, a liquid flow cell TEM holder equipped with a miniaturized viewing chamber has been developed and applied to the detection of high atomic number labeling nanoparticles by scanning TEM high-angle annular dark-field imaging. (nist.gov)
• Here we demonstrate the application of the flow cell system for conventional TEM bright-field imaging of immobilized nanoparticles in a liquid of micrometer thickness. (nist.gov)
• Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. (beilstein-journals.org)
• Selectivity can be established using immobilized biomolecules on nanoparticles for interaction with proteins, peptides and nucleic acid sequences. (utoronto.ca)

Biosensor3

• Our aim is to fabricate a sensitive whole-cell oxidative stress biosensor which has improved properties that address the mentioned weaknesses. (elsevierpure.com)
• In this paper, we report a novel high-throughput whole-cell biosensor fabricated by immobilizing roGFP2 expressing Escherichia coli cells in a k-carrageenan matrix, for the detection of oxidative stress challenged by metalloid compounds. (elsevierpure.com)
• Ooi, L, Heng, LY & Mori, IC 2015, ' A high-throughput oxidative stress biosensor based on Escherichia coli rOGFP2 cells immobilized in a k-Carrageenan matrix ', Sensors (Switzerland) , vol. 15, no. 2, pp. 2354-2368. (elsevierpure.com)

Protein15

• Recombinant Protein;20UG;ActiveMax Human IL-4, Tag Free (IL4-H4218) is expressed from human 293 cells (HEK293). (fishersci.com)
• Complementing our research on virus cell entry is our effort to define the mechanisms of virus particle assembly and regulation of the assembly outcomes (i.e., structural features of virus particles such as shape, size, or viral protein incorporation). (nih.gov)
• 1. pim-1 proto-oncogene expression in anti-CD3-mediated T cell activation is associated with protein kinase C activation and is independent of Raf-1. (nih.gov)
• 8. Age-related reductions in the activation of mitogen-activated protein kinases p44mapk/ERK1 and p42mapk/ERK2 in human T cells stimulated via ligation of the T cell receptor complex. (nih.gov)
• 11. The role of protein kinase C in transmembrane signaling by the T cell antigen receptor complex. (nih.gov)
• 14. Induction of glucose-regulated protein 78 by chronic hypoxia in human gastric tumor cells through a protein kinase C-epsilon/ERK/AP-1 signaling cascade. (nih.gov)
• The active when overexpressed from a multicopy plasmid, DinI form of RecA is a protein filament formed on ssDNA in conferred significant UV sensitivity to cells and pre- an ATP-dependent fashion. (nih.gov)
• Variant protein boosts induced GCs with a higher proportion of IgM+ B cells. (elifesciences.org)
• The most variant protein re-stimulated GCs with the highest proportion of IgM+ cells with the most diverse, least mutated V-genes and with a slower but efficient serum antibody response. (elifesciences.org)
• Without the original protein injection, the response without the immature memory B cells was not as efficient. (elifesciences.org)
• The body therefore has a pool of memory B cells that can recognise a wider range of virus protein variants than the ones that caused the first immune reaction. (elifesciences.org)
• Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). (gbiosciences.com)
• Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. (gbiosciences.com)
• Immobilized Human IgG is affinity resin used to purify Protein A and Protein A tagged recombinant proteins and is suitable for tandem affinity purific. (gbiosciences.com)
• In this study, we show in human and frog cells that Plk4 interacts with the centrosome protein Cep152, the orthologue of Drosophila melanogaster Asterless. (rupress.org)

Reactive3

• This reveals a new process of antibody memory, that IgM memory cells with fewer mutations participate in secondary responses to variant antigens, demonstrating how the hierarchical structure of B-cell memory is used and indicating the potential and limits of cross-reactive antibody based immunity. (elifesciences.org)
• Mechanistically, we highlight the significance of immobilized peroxidase reactive intermediates vs diffusible small molecule oxidants (hypochlorous and hypobromous acids) for the overall oxidative biodegradation process in neutrophils and eosinophils. (cdc.gov)
• The activation of XO and reduced Tetrahydrobiopterin (BH 4 ) levels in endothelial cells leads to increased reactive oxygen species (ROS) formation, which in turn leads to endothelial nitric oxide synthase (eNOS) uncoupling and further production of superoxide (Thomas, et al 2010). (justia.com)

Antigen2

• 12. Regulation of gamma T-cell antigen receptor expression by intracellular calcium in acute lymphoblastic leukemia cell line DND41. (nih.gov)
• Lipoprotein Lipase/LPL was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)

Proteins4

• Compared to the fully mature cells, the immature memory B cells were much better at recognizing the variants of the proteins, and these cells then multiplied and mounted an immune response. (elifesciences.org)
• The multiple molecular variant forms of AFP are discussed in relation to published reports of AFP binding proteins and cell surface receptors. (atlasgeneticsoncology.org)
• Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (who.int)
• The Sickled-shaped erythrocytes together with endothelial cells, activated leukocytes, platelets and plasma proteins participate in the multistep vaso-occlusion process (Frenette 2002). (justia.com)

Biol2

• Cell Biol. (nature.com)
• J Cell Biol (2010) 191 (4): 721-729. (rupress.org)

Sickle cell di6

• Dr. Keith Hoots, the Division Director at the Division of Blood Diseases and Resources (DBDR) opened the meeting by greeting the Sickle Cell Disease Advisory committee (SCDAC) members. (nih.gov)
• In 1999 there were 2 major obstacles for curing adults with sickle cell disease. (nih.gov)
• The present invention includes embodiments for treatment and/or prevention of sickle cell disease that employ Hydroxyfasudil or Isocoronarin D alone or either in conjunction with each other or an inducer of HbF production. (justia.com)
• In particular aspects, the field of the present invention includes treatment and/or prevention of a blood disorder, such as sickle cell disease. (justia.com)
• Sickle cell disease (SCD) is the most common life-threatening monogenic disorder in the world with statistics indicating that approximately 80% (230,000) of children affected globally are born in sub-Saharan Africa (Modell and Darlison 2008). (justia.com)
• Sickle cell disease (SCD) can arise from a single point mutation that causes erythrocyte deformation or sickle-shaped erythrocytes (Ingram 1957). (justia.com)

Endothelial cells1

• A coating strategy to enhance endothelial restoration by utilizing 'pro-healing' substances not eluted by the stent, but which attract endothelial cells, may reduce the thrombogenicity of the device. (medscape.com)

Detection1

• Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1. (boku.ac.at)

Interaction3

• 18. Interaction of CD4:lck with the T cell receptor/CD3 complex induces early signaling events in the absence of CD45 tyrosine phosphatase. (nih.gov)
• When the suramin-conjugated beads were added to the cells on cell culture inserts, which preclude an interaction with the cell surface but allow interaction with the culture medium, there was no effect on proliferation. (nih.gov)
• Suramin need not enter NCI-H596 cells to exert its growth-stimulatory effect, nor is this effect mediated by an interaction with soluble growth factors. (nih.gov)

Biology2

• We combine molecular virology, in vitro reconstitution, biophysics, and quantitative cell biology to answer difficult-to-tackle mechanistic questions in virology and build upon those discoveries to ultimately enable prediction and prevention of undesirable viral adaptation (e.g., drug resistance, immune escape, or pandemic adaptation). (nih.gov)
• The field of the present invention includes at least biology, cell biology, and medicine. (justia.com)

Membrane1

• Viruses initiate infection by delivering the genomes from within the viral capsid or a membrane envelope to the target cell. (nih.gov)

Peptides1

• We further observed that the addition of cell-penetrating peptides, Tat and T7, did not increase BBB penetration above the aptamer-loaded LNPs alone. (beilstein-journals.org)

Stimulation2

• 4. Rapid activation of C-Raf-1 after stimulation of the T-cell receptor or the muscarinic receptor type 1 in resting T cells. (nih.gov)
• Mechanisms of growth stimulation by suramin in non-small-cell lung cancer cell lines. (nih.gov)

Oxidative2

• Here, we review the role and contribution of different oxidative enzymes of inflammatory cells - myeloperoxidase, eosinophil peroxidase, lactoperoxidase, hemoglobin, and xanthine oxidase - to the reactions of nanoparticle biodegradation. (cdc.gov)
• We consider possible involvement of oxidative machinery of other professional phagocytes such as microglial cells, myeloid-derived suppressor cells, in the context of biodegradation relevant to targeted drug delivery. (cdc.gov)

Attachment1

• Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. (nih.gov)

Antigens1

• Vaccines induce memory B-cells that provide high affinity secondary antibody responses to identical antigens. (elifesciences.org)

Receptor1

• 10. Activation of nuclear factor-kappaB via T cell receptor requires a Raf kinase and Ca2+ influx. (nih.gov)

Proliferation3

• No. IL4-H4218) stimulates the proliferation of TF-1 human erythroleukemic cells. (fishersci.com)
• The NPs promoted proliferation of baby hamster kidney fibroblasts (BHK-21 cells ) and mouse osteoblastic MC3T3-E1 cells at a certain dose range, although they inhibited proliferation of rat pheochromocytoma (PC-12) cells . (bvsalud.org)
• These results demonstrated that the effect of the NPs on cell proliferation was dependent on the cell type and dose , the details of which should be investigated in a future study. (bvsalud.org)

20002

• In an effort to target LNPs composed of an ionizable cationic lipid (DLin-MC3-DMA), cholesterol, the phospholipid 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), and 1,2-dimyristoyl- rac -glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG 2000) to particular cell types, as well as to generate LNPs that can cross the BBB, we developed and assessed two approaches. (beilstein-journals.org)
• 2000. N-glycans influence adhesive and invasive behavior in vitro of three metastatic cell. (bchi.lt)

Complementing1

• Complementing the microfluidic cell culture work, surface enhanced Raman spectroscopy (SERS), a label-free vibrational spectroscopic technique that lends itself well to use in aqueous systems, was used to detect bacterial signaling molecules. (umd.edu)

Bacterial2

• This research is expected to provide a new direction in performing high-throughput environmental toxicity screening with living bacterial cells which is capable of measuring the bioavailability and toxicity of environmental stressors in a friction of a second. (elsevierpure.com)
• When a bacterial cell is subjected to the harmful action leading to the termination of the SOS response. (nih.gov)

Receptors1

• We are further working to define how the structural organization of virus particles might enable entry into cells not displaying fitting receptors. (nih.gov)

Gastrointestinal2

• A major factor in alcohol-related disease stems from the ability of alcohol to compromise the natural barrier function of cells in the gastrointestinal tract, increasing the movement of toxins from the intestines to other organs in the body. (nih.gov)
• Such AFP peptide segments could potentially serve as delivery agents which could target vascular, neuroendocrine, or gastrointestinal cells. (atlasgeneticsoncology.org)

Immune3

• When a virus enters the body, a group of immune cells known as B cells gets activated. (elifesciences.org)
• If the same virus enters the organism again, the memory B cells rapidly identify it and produce a quicker and more efficient immune response than during the first attack. (elifesciences.org)
• As a former astronaut, Hughes-Fulford aims to apply lessons learned from studies of immune cells in microgravity to a new model for investigating the loss of immune response in older women and men. (nih.gov)

Suitable1

• Biosensors fabricated with whole-cell bacteria appear to be suitable for detecting bioavailability and toxicity effects of the chemical(s) of concern, but they are usually reported to have drawbacks like long response times (ranging from hours to days), narrow dynamic range and instability during long term storage. (elsevierpure.com)

Behavior1

• The model exhibited a perfect correlation between the predicted and experimental data, and this stipulates that ANFIS can predict characteristic behavior of fuel cell performance with very high accuracy. (mdpi.com)

Mechanism2

• Apoptosis as a mechanism for myonuclear loss with atrophy is controversial, whereas cell death of satellite cells has not been considered. (frontiersin.org)
• This dissertation describes the development of a microfluidic system for immobilizing and culturing of cells that also enables control over the genetic composition of the bacteria and their subsequent response to environmental stimuli via a new nonviral nucleic acid delivery mechanism. (umd.edu)

Recognize4

• Viral outbreaks and pandemics arise when animal viruses adapt so that they can recognize and use human cells as their host. (nih.gov)
• During the infection some of these B cells become 'memory B cells', having gone through a maturation process that hones their ability to specifically recognize this particular microbe. (elifesciences.org)
• However memory B cells may not be able to recognize a previous intruder if it has changed too much. (elifesciences.org)
• Some cells are fully mature and can quickly recognize the original virus. (elifesciences.org)

Enables1

• This insight led to a model where the pre-existing diversity in virus particle size in pleomorphic (mixed-shape) viruses enables viral adaptation to cell-entry pressure in a way that does not require an initiating genetic change. (nih.gov)

Molecular2

• In their pursuit of understanding cellular function, biologists seek to observe the processes that allow cells to maintain homeostasis and react dynamically to internal and external cues-on both a molecular scale and inside structurally intact, ideally living specimens. (nature.com)
• Molecular signaling pathways activated in muscle fibers by disuse are rarely considered within satellite cells themselves despite similar exposure to unloading or low mechanical load. (frontiersin.org)

Activation1

• 20. CD4 ligands inhibit the formation of multifunctional transduction complexes involved in T cell activation. (nih.gov)

Process3

• But others have not finished their maturation process: these cells are less focussed, and cannot target the original microbe with the same exact precision shown by mature memory cells. (elifesciences.org)
• These cells play a key role in bone remodeling, a process that is vital to skeletal health. (nih.gov)
• Endothelial progenitor cells have also been identified as a key factor in the re-endothelialization process after stent implantation. (medscape.com)

High3

• Influenza cell-entry glycoprotein hemagglutinin (HA) is present at a high density on virus particles. (nih.gov)
• Transmission electron microscopy (TEM) provides a method for imaging with the requisite spatial resolution, but such imaging can be accomplished only with a means of isolating the liquid environment from the high vacuum of the instrument column, such as an impermeable cell with electron transparent windows. (nist.gov)
• Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept. (boku.ac.at)

Mechanisms1

• The purpose of this study was to examine the mechanisms by which suramin exerts this growth-stimulatory effect in NSCLC cells. (nih.gov)

Addition1

• NCI-H596 cells were treated with agarose-immobilized suramin, directly or by addition on cell culture inserts, after which growth was determined by [3H]thymidine incorporation. (nih.gov)

Growth3

• Satellite cells are particularly responsive to myostatin and to growth factors. (frontiersin.org)
• We have reported that suramin, at dose concentrations higher than 140 microM, exerts growth-stimulatory effects in several non-small-cell lung cancer (NSCLC) cell lines. (nih.gov)
• Agarose-immobilized suramin stimulated NCI-H596 cell growth, but only when added directly to the cells. (nih.gov)

Patients1

• Paola Divieti, M.D., Ph.D., Massachusetts General Hospital/Harvard Medical School, Boston: Weight-bearing activities contribute to the development and maintenance of bone mass, while weightlessness and immobility - as experienced by the astronauts and bedridden and immobilized patients - can result in bone loss and a weakened skeleton. (nih.gov)

Data1

• Blank cells indicate that no data were available in the literature. (nih.gov)

Effects1

• Electrophysiological studies in mammalian cells transfected with human K v 7.2 and/or K v 7.3 cDNAs revealed that each of these four mutations stabilized the activated state of the channel, thereby producing gain-of-function effects, which are opposite to the loss-of-function effects produced by previously found mutations. (jneurosci.org)

Culture1

• Using microgravity three-dimensional cell culture models, McCole plans to generate insights regarding the barrier properties of the intestines, and to explore how the absence of gravity affects alcohol's ability to diminish this barrier. (nih.gov)

Type2

• Osteocytes, the most common type of bone cell, are believed to have gravity-sensing abilities. (nih.gov)
• The most common type of SCD is sickle cell anemia (SCA) (also referred to as HbSS or SS disease or hemoglobin S) in which there is homozygosity for the mutation that causes HbS. (justia.com)

Line3

• Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. (rndsystems.com)
• Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. (rndsystems.com)
• Lipoprotein Lipase/LPL in SH‑SY5Y Human Cell Line. (rndsystems.com)

Responses1

• Skeletal muscle stem cells (satellite cells) and myonuclei are integrally involved in skeletal muscle responses to environmental changes that induce atrophy. (frontiersin.org)

Body2

• Osteoclasts are the only cells in the body that have a bone-destroying capability. (hindawi.com)
• Mucus trapping can prevent pathogens from infecting cells and accelerate their removal from the body. (nih.gov)

Entry1

• We report herein a CCR5-selective RNA aptamer that acts to facilitate entry through a simplified BBB model and that drives the uptake of LNPs into CCR5-expressing cells, while the gp160 aptamer did not. (beilstein-journals.org)

Free1

• Immobilized ActiveMax Human IL-4, Tag Free (Cat. (fishersci.com)

Single1

• Real- time measurements on the spatial and temporal fluctuations of single molecules in living cells, which are not possible using other methods, are a major goal of this initiative. (nih.gov)