Phenylpyruvic Acids
Phenylalanine Ammonia-Lyase
Phenylalanine
Phenylalanine Hydroxylase
Transaminases
Allied Health Occupations
Library Collection Development
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
Textbooks as Topic
Nanowires
Vitallium
Nanostructures
Polyesters
Collagen
Surface Properties
Microalgae
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Biofuels
Formate Dehydrogenases
Flavoproteins that catalyze reversibly the reduction of carbon dioxide to formate. Many compounds can act as acceptors, but the only physiologically active acceptor is NAD. The enzymes are active in the fermentation of sugars and other compounds to carbon dioxide and are the key enzymes in obtaining energy when bacteria are grown on formate as the main carbon source. They have been purified from bovine blood. EC 1.2.1.2.
Epinephrine
The active sympathomimetic hormone from the ADRENAL MEDULLA. It stimulates both the alpha- and beta- adrenergic systems, causes systemic VASOCONSTRICTION and gastrointestinal relaxation, stimulates the HEART, and dilates BRONCHI and cerebral vessels. It is used in ASTHMA and CARDIAC FAILURE and to delay absorption of local ANESTHETICS.
Adrenal Glands
Malaria
A protozoan disease caused in humans by four species of the PLASMODIUM genus: PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM OVALE; and PLASMODIUM MALARIAE; and transmitted by the bite of an infected female mosquito of the genus ANOPHELES. Malaria is endemic in parts of Asia, Africa, Central and South America, Oceania, and certain Caribbean islands. It is characterized by extreme exhaustion associated with paroxysms of high FEVER; SWEATING; shaking CHILLS; and ANEMIA. Malaria in ANIMALS is caused by other species of plasmodia.
Cell Movement
Sympathetic Nervous System
The thoracolumbar division of the autonomic nervous system. Sympathetic preganglionic fibers originate in neurons of the intermediolateral column of the spinal cord and project to the paravertebral and prevertebral ganglia, which in turn project to target organs. The sympathetic nervous system mediates the body's response to stressful situations, i.e., the fight or flight reactions. It often acts reciprocally to the parasympathetic system.
Leukocytes
Milk
Dairy Products
Triticum
Lactation
Fermentation
Dietary Fiber
Probiotics
Live microbial DIETARY SUPPLEMENTS which beneficially affect the host animal by improving its intestinal microbial balance. Antibiotics and other related compounds are not included in this definition. In humans, lactobacilli are commonly used as probiotics, either as single species or in mixed culture with other bacteria. Other genera that have been used are bifidobacteria and streptococci. (J. Nutr. 1995;125:1401-12)
Spectroscopy, Electron Energy-Loss
A technique for analysis of the chemical composition of molecules. A substance is bombarded with monochromatic ELECTRONS. Some of the electrons passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The energy loss is element dependent. Analysis of the energy loss spectrum reveals the elemental composition of a specimen. ENERGY-FILTERED TRANSMISSION ELECTRON MICROSCOPY is a type of electron energy loss spectroscopy carried out in electron microscopes specially outfitted to analyze the spectrum of electron energy loss.
Glycolysis
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Neoplasms
Iridium Radioisotopes
Glycine Hydroxymethyltransferase
Fructose-Bisphosphate Aldolase
Threonine Dehydratase
A pyridoxal-phosphate protein that catalyzes the deamination of THREONINE to 2-ketobutyrate and AMMONIA. The role of this enzyme can be biosynthetic or biodegradative. In the former role it supplies 2-ketobutyrate required for ISOLEUCINE biosynthesis, while in the latter it is only involved in the breakdown of threonine to supply energy. This enzyme was formerly listed as EC 4.2.1.16.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Threonine
Tromethamine
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
Immobilization patterns and dynamics of acetate-utilizing methanogens immobilized in sterile granular sludge in upflow anaerobic sludge blanket reactors. (1/189)
Sterile granular sludge was inoculated with either Methanosarcina mazeii S-6, Methanosaeta concilii GP-6, or both species in acetate-fed upflow anaerobic sludge blanket (UASB) reactors to investigate the immobilization patterns and dynamics of aceticlastic methanogens in granular sludge. After several months of reactor operation, the methanogens were immobilized, either separately or together. The fastest immobilization was observed in the reactor containing M. mazeii S-6. The highest effluent concentration of acetate was observed in the reactor with only M. mazeii S-6 immobilized, while the lowest effluent concentration of acetate was observed in the reactor where both types of methanogens were immobilized together. No changes were observed in the kinetic parameters (Ks and mumax) of immobilized M. concilii GP-6 or M. mazeii S-6 compared with suspended cultures, indicating that immobilization does not affect the growth kinetics of these methanogens. An enzyme-linked immunosorbent assay using polyclonal antibodies against either M. concilii GP-6 or M. mazeii S-6 showed significant variations in the two methanogenic populations in the different reactors. Polyclonal antibodies were further used to study the spatial distribution of the two methanogens. M. concilii GP-6 was immobilized only on existing support material without any specific pattern. M. mazeii S-6, however, showed a different immobilization pattern: large clumps were formed when the concentration of acetate was high, but where the acetate concentration was low this strain was immobilized on support material as single cells or small clumps. The data clearly show that the two aceticlastic methanogens immobilize differently in UASB systems, depending on the conditions found throughout the UASB reactor. (+info)Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (2/189)
Viable cells of Pseudomonas aeruginosa were entrapped in alginate gel layers and incubated in a minimal glucose (15 g/L)-yeast extract (2 g/L)-salt medium to form artificial biofilm-like structures. After cultivation for 2 days, the biomass distribution inside the polymer was highly heterogeneous. The cell number reached approximately 1011 cells/g gel in the outer regions of the gel structures whereas the inner areas were less colonized (c. 10(8) cells g/gel). Killing of immobilized organisms by imipenem and tobramycin were compared with free-cell experiments (inoculum c. 10(9) cells/mL). Sessile-like bacteria displayed a higher resistance to the two antibiotics used alone or in combination than did suspended cells. Exposure for 10 h to 20 x MIC imipenem and 15 x MIC tobramycin reduced the number of viable immobilized bacteria to 0.3% and 3%, respectively, of the initial cell population, whereas these antibiotic concentrations were much more efficient (bactericidal) against free-cell cultures (5 log kill in 6 h). A synergic effect of tobramycin and imipenem was detected on bacterial suspensions but not on biofilm-like structures. Effective diffusivity measurements showed that the diffusion of imipenem in the alginate layer was not hindered. A slight but significant enhancement of beta-lactamase induction in immobilized cells as compared with their suspended counterparts was insufficient to explain the high resistance of sessile-like bacteria. (+info)Distribution of Nitrosomonas europaea and Paracoccus denitrificans immobilized in tubular polymeric gel for nitrogen removal. (3/189)
To improve the cooperative removal of nitrogen by Nitrosomonas europaea and Paracoccus denitrificans, we controlled their distribution in a tubular gel. When ethanol was supplied inside the tubular gel as an electron donor, their distributions overlapped in the external region of the gel. By changing the electron donor from ethanol to gaseous hydrogen, the distribution of P. denitrificans shifted to the inside of the tube and was separated from that of N. europaea. The separation resulted in an increase of the oxidation rate of ammonia by 25%. (+info)Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. (4/189)
Bifidobacterium longum KCTC 3128 and HLC 3742 were independently immobilized (entrapped) in calcium alginate beads containing 2, 3, and 4% sodium alginate. When the bifidobacteria entrapped in calcium alginate beads were exposed to simulated gastric juices and a bile salt solution, the death rate of the cells in the beads decreased proportionally with an increase in both the alginate gel concentration and bead size. The initial cell numbers in the beads affected the numbers of survivors after exposure to these solutions; however, the death rates of the viable cells were not affected. Accordingly, a mathematical model was formulated which expressed the influences of several parameters (gel concentration, bead size, and initial cell numbers) on the survival of entrapped bifidobacteria after sequential exposure to simulated gastric juices followed by a bile salt solution. The model proposed in this paper may be useful for estimating the survival of bifidobacteria in beads and establishing optimal entrapment conditions. (+info)Identification of a novel glycoprotein-binding activity in Streptococcus pyogenes regulated by the mga gene. (5/189)
The interaction between Streptococcus pyogenes and the host cell surface is not completely understood. Characterization of the adhesion mechanisms of the bacterium to the host cell surface is needed in order to develop new vaccines and anti-adhesion drugs. The presence of glycoprotein-binding activities among streptococcal strains was investigated. An activity binding to thyroglobulin, fetuin, asialofetuin and mucin but not non-glycosylated proteins was found to be present in the majority of the S. pyogenes strains studied. Cross-inhibition experiments suggested that the glycoproteins share a common structure recognized by the bacteria. The glycoprotein-binding activity was found to be proteinaceous, tightly attached to the bacterial surface and it also mediated the adherence of bacteria to solid surfaces coated with glycoproteins. The activity was found by transposon mutagenesis and complementation to be regulated by the multiple-gene regulator Mga, which has been implicated as a regulator of S. pyogenes virulence factors. (+info)Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface. (6/189)
Adhesion of Candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. C. albicans cells also adhere to several species of oral streptococci including Streptococcus gordonii, Streptococcus oralis and Streptococcus sanguinis in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of C. albicans cells by up to 2-3-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which C. albicans cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of S. gordonii cells but not to S. oralis cells. Parotid saliva that was pre-adsorbed with S. gordonii cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of C. albicans compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. C. albicans cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of C. albicans to S. gordonii cells. The results demonstrate that C. albicans cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote C. albicans adhesion and oral colonization. (+info)Response kinetics of tethered Rhodobacter sphaeroides to changes in light intensity. (7/189)
Rhodobacter sphaeroides can swim toward a wide range of attractants (a process known as taxis), propelled by a single rotating flagellum. The reversals of motor direction that cause tumbles in Eschericia coli taxis are replaced by brief motor stops, and taxis is controlled by a complex sensory system with multiple homologues of the E. coli sensory proteins. We tethered photosynthetically grown cells of R. sphaeroides by their flagella and measured the response of the flagellar motor to changes in light intensity. The unstimulated bias (probability of not being stopped) was significantly larger than the bias of tethered E. coli but similar to the probability of not tumbling in swimming E. coli. Otherwise, the step and impulse responses were the same as those of tethered E. coli to chemical attractants. This indicates that the single motor and multiple sensory signaling pathways in R. sphaeroides generate the same swimming response as several motors and a single pathway in E. coli, and that the response of the single motor is directly observable in the swimming pattern. Photo-responses were larger in the presence of cyanide or the uncoupler carbonyl cyanide 4-trifluoromethoxyphenylhydrazone (FCCP), consistent with the photo-response being detected via changes in the rate of electron transport. (+info)Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system. (8/189)
A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain GCH1 grew on propachlor with a generation time of 4.2 h and a rate of substrate utilization of 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but was subject to catabolite expression. Acetanilide was a growth substrate with a yield of 0.56 +/- 0.02 mg of protein micromol(-1). GCH1 strain cells were immobilized by adsorption onto a ceramic support and were used as biocatalysts in an immobilized cell system. Propachlor elimination reached 98%, with a retention time of 3 h and an initial organic load of 0.5 mM propachlor. The viability of immobilized cells increased 34-fold after 120 days of bioreactor operation. (+info)
High alcohol production by repeated batch fermentation using an immobilized osmotolerant Saccharomyces cerevisiae
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Immobilized Cells. Berlin, Heidelberg: Springer Berlin Heidelberg. ISBN 3-642-56891-2.CS1 maint: extra text: authors list (link ... proceedings of a satellite symposium of the 3rd International Congress on Cell Biology, Gifu, Japan, 22-24 August 1984. Utrecht ...
Arthrobacter globiformis
1996). Immobilized Cells Basics and Applications. Burlington: Elsevier. ISBN 0-08-053447-3. Emanuel Goldman; Lorrence H. Green ...
Succinyl coenzyme A synthetase
Since the cells do not have a functional citric acid cycle, acidosis results because cells are forced to choose lactic acid ... Wider de Xifra E, del C Batlle AM (Mar 1978). "Porphyrin biosynthesis: immobilized enzymes and ligands. VI. Studies on succinyl ... An interesting study using pigeon cells showed that GTP specific SCSs were located in pigeon liver cells, and ATP specific SCSs ... and it is located within the mitochondrial matrix of a cell. Succinyl CoA synthetase catalyzes the following reversible ...
Biological photovoltaics
"A photoelectrochemical cell using immobilized photosynthetic membranes". Bioelectrochemistry and Bioenergetics. 22 (3): 391-401 ... Like other fuel cells, biological photovoltaic systems are divided into anodic and cathodic half-cells. Oxygenic photosynthetic ... The insulating outer membranes of whole cells impedes electron transfer from the sites of electron generation inside the cell ... and are sometimes also called photo-microbial fuel cells or "living solar cells". In a biological photovoltaic system, ...
Arthrobacter citreus
Karigar, C; Mahesh, A; Nagenahalli, M; Yun, DJ (February 2006). "Phenol degradation by immobilized cells of Arthrobacter ... "Phenol degradation by immobilized cells of Arthrobacter citreus". Biodegradation. 17 (1): 47-55. doi:10.1007/s10532-005-3048-y ...
Ribosome profiling
Lyse the cells or tissue and isolate the mRNA molecules bound to ribosomes. Immobilize complexes. This is commonly performed ... 2008). "A translational profiling approach for the molecular characterization of CNS cell types". Cell. 135 (4): 738-48. doi: ... This allows the starting codon of the mRNAs throughout the cell lysate to be analyzed, which has been used to determine non-AUG ... It produces a "global snapshot" of all the ribosomes actively translating in a cell at a particular moment, known as a ...
Luciferase
Biotinylation will immobilize luciferase on the cell-surface by binding to a streptavidin-biotin complex. This allows ... Different types of cells (e.g. bone marrow stem cells, T-cells) can be engineered to express a luciferase allowing their non- ... "Cell-surface-localized ATP detection with immobilized firefly luciferase". Analytical Biochemistry. 352 (1): 61-67. doi:10.1016 ... Huh S, Lee J, Jung E, Kim SC, Kang JI, Lee J, Kim YW, Sung YK, Kang HK, Park D (Jun 2009). "A cell-based system for screening ...
Bovine serum albumin
Zhang M, Desai T, Ferrari M (May 1998). "Proteins and cells on PEG immobilized silicon surfaces" (PDF). Biomaterials. 19 (10): ... BSA is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes ... BSA is also the main constituent of fetal bovine serum, a common cell culture medium. Acceptable daily intake Protein allergy ... During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often ...
Biomolecular engineering
Examples of types of biomolecules that are immobilized are enzymes, organelles, and complete cells. Biomolecules can be ... Cellular engineering - Design of new cells using recombinant DNA and development of procedures to allow normal cells to adhere ... Attention is also given to the rudiments of engineered biomolecules in cell signaling, cell growth kinetics, biochemical ... Zhou, Quinn Z. K; Chen, Xiao Dong (2001). "Effects of temperature and pH on the catalytic activity of the immobilized β- ...
Milica Radisic
Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells ... Her work centers on using human embryonic and induced pluripotent stem cells to develop a heart patch that could be used to ... Radisic is known for creating beating heart tissue in a dish using human-induced pluripotent stem cells. Her research has led ... Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes SS Nunes, JW Miklas, J Liu, R Aschar- ...
VRLA battery
VRLA cells have the same chemistry, except the electrolyte is immobilized. In AGM this is accomplished with a fiberglass mat; ... When a cell discharges, the lead and diluted acid undergo a chemical reaction that produces lead sulfate and water. When a cell ... There are two primary types of VRLA batteries, absorbent glass mat (AGM) and gel cell. Gel cells add silica dust to the ... The gel cells were less likely to leak when the portable set was handled roughly. A modern gel battery is a VRLA battery with a ...
Corynebacterium
"Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells". Biotechnology and ... It is an acute and contagious infection characterized by pseudomembranes of dead epithelial cells, white blood cells, red blood ... The cell wall is distinctive, with a predominance of mesodiaminopimelic acid in the murein wall and many repetitions of ... Keddie, R. M.; Cure, G. L. (1977). "The Cell Wall Composition and Distribution of Free Mycolic Acids in Named Strains of ...
Electric battery
... is popular in the automotive industry as a replacement for the lead-acid wet cell. The VRLA battery uses an immobilized ... Other primary wet cells are the Leclanche cell, Grove cell, Bunsen cell, Chromic acid cell, Clark cell, and Weston cell. The ... From top to bottom: a large 4.5-volt 3R12 battery, a D Cell, a C cell, an AA cell, an AAA cell, an AAAA cell, an A23 battery, a ... fuel cells, flow cells and voltaic piles.[29] Wet cell. A wet cell battery has a liquid electrolyte. Other names are flooded ...
Albendazole
Cell motility, maintenance of cell shape, and intracellular transport are also disrupted. At higher concentrations, it disrupts ... Due to diminished ATP production, the parasite is immobilized and eventually dies. Some parasites have evolved to have some ... drop in white blood cells, red blood cells, and platelets), hepatitis, acute liver failure, and acute kidney injury, ... September 2012). "A cell-based screen reveals that the albendazole metabolite, albendazole sulfone, targets Wolbachia". PLOS ...
VRLA battery
"Wet cell" type VRLA's contain acid in liquid form. In AGM and gel type VRLA's, the electrolyte is immobilized. In AGM this is ... There are three primary types of VRLA batteries, sealed VR wet cell, absorbent glass mat (AGM) and gel cell.[2] Gel cells add ... The immobilized electrolyte in VRLA batteries (AGM and gel types) addresses these problems. Conversely, VRLA cells can't be ... Rectangular cells may have valves set to operate as low as 1 or 2 psi; round spiral cells, with metal external containers, can ...
CyTOF
Tagged antibodies may be in solution, conjugated to beads, or surface immobilized. The cell staining follows the same ... Spitzer MH, Nolan GP (May 2016). "Mass Cytometry: Single Cells, Many Features". Cell. 165 (4): 780-91. doi:10.1016/j.cell. ... cells can be probed with rhodium, an intercalator which can only penetrate dead cells. Then all cells are fixed and stained ... The cell introduction method of the mass cytometer is an aerosol splitter injection. The cells are then captured in a stream of ...
Titanate nanosheet
TiNSs may also be used for immobilizing biomolecules. When a monolayer of hemoglobin is intercalated into TiNSs, the electron ... Further, TiNSs potential as an electrocatalyst may enhance fuel cell efficiency during fuel oxidation. Similarly, intercalated ...
Rotator cuff tear
Mesenchymal stem cells have no convincing evidence for their use overall, with quality human trials lacking. The greater ... First, the arm is immobilized so that the muscle can heal. Second, when appropriate, a therapist assists with passive exercises ... Pas (2017). "No evidence for the use of stem cell therapy for tendon disorders: a systematic review". Br J Sports Med. 51 (13 ... These include injecting an individual's own stem cells, growth factors or platelet rich plasma (PRP) into the repair site, and ...
Laminaria
Nestle, Nikolaus F.E.I.; Kimich, Rainer (March 1996). "NMR Imaging of Heavy Metal Absorption in Alginate, Immobilized Cells, ... These structures are very similar to mesophyll cells found in higher plant leaves. A laminaria stick may be used to slowly ...
Arthrobacter rhombi
Elangovan, R.; Philip, Ligy; Chandraraj, K. (23 January 2009). "Hexavalent Chromium Reduction by Free and Immobilized Cell-free ...
Reactive bonding
In addition disposable microfluidic devices with sensing function and immobilized cells can be fabricated. Wafer bonding Direct ...
Chlorella vulgaris
... and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth- ... Morris, H. J., Almarales, A., Carrillo, O., & Bermúdez, R. C. (2008). "Utilisation of Chlorella vulgaris cell biomass for the ... "Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum ...
Ecological competence
Controlled release of immobilized cells as a strategy to regulate ecological competence of inocula. In Biotechnics/wastewater ( ... Increased plasmid stability leads to a higher ecological competence due to added spatial organization and regulated cell ...
Industrial dye degradation
Immobilized fungal cells are more resistant to environmental stress and cells can be used repeatedly. Many dyes, specifically ... Kinetic Study of an Immobilized Iron Oxide for Catalytic Degradation of Azo Dye Reactive Black B with Catalytic Decomposition ...
Contactin 2
Rader C, Stoeckli ET, Ziegler U, Osterwalder T, Kunz B, Sonderegger P (Jul 1993). "Cell-cell adhesion by homophilic interaction ... Kuhn TB, Stoeckli ET, Condrau MA, Rathjen FG, Sonderegger P (Nov 1991). "Neurite outgrowth on immobilized axonin-1 is mediated ... Pise-Masison CA, Dittmer J, Clemens KE, Brady JN (Mar 1997). "Physical and functional interaction between the human T-cell ... Pise-Masison CA, Dittmer J, Clemens KE, Brady JN (Mar 1997). "Physical and functional interaction between the human T-cell ...
Phosphoproteomics
In SILAC, cells are cultured separately in the presence of different isotopically labeled amino acids for several cell ... IMAC enrichment is based on phosphate affinity for immobilized metal chelated to the resin. SCX separates phosphorylated from ... In one study design, cells are exposed to SILAC labelling and then stimulated by a specific growth factor. The cells are ... A sample large-scale phosphoproteomic analysis includes cultured cells undergo SILAC encoding; cells are stimulated with factor ...
Phospholipase A2
Venom phospholipases help to immobilize prey by promoting cell lysis[citation needed]. In mice, group III sPLA2 are involved in ... In normal brain cells, PLA2 regulation accounts for a balance between arachidonic acid's conversion into proinflammatory ... PLA2 action can release histamine from rat peritoneal mast cells. It also causes histamine release in human basophils. Due to ... Argiolas A, Pisano JJ (Nov 1983). "Facilitation of phospholipase A2 activity by mastoparans, a new class of mast cell ...
Coral
The polyp's tentacles immobilize or kill prey using stinging cells called nematocysts. These cells carry venom which they ... The body of the polyp may be roughly compared in a structure to a sac, the wall of which is composed of two layers of cells. ... The mesoglea can contain skeletal elements derived from cells migrated from ectoderm. The sac-like body built up in this way is ... Although some corals are able to catch plankton and small fish using stinging cells on their tentacles, most corals obtain the ...
Biosensor
Cells tend to attach to the surface so they can be easily immobilized. Compared to organelles they remain active for longer ... A live-cell biosensor for cAMP can be used in non-lysed cells with the additional advantage of multiple reads to study the ... Advantages of tissues as biosensors include the following: easier to immobilize compared to cells and organelles the higher ... There is very high selectivity for transferrin receptors because they are over-expressed in cancer cells. If cells have high ...
Streptomyces phaeochromogenes
Alfred, Olson (1974). Immobilized Enzymes in Food and Microbial Processes. Boston, MA: Springer US. ISBN 1-4684-2088-7. Atta-ur ... Kumakura, Minoru; Yoshida, Masaru; Kaetsu, Isao (1978). "Immobilization of Streptomyces phaeochromogenes cells at a high ...
Muscle atrophy
... when the patient has a primary injury such as an immobilized broken bone (set in a cast or immobilized in traction), for ... "satellite cells" which help to regenerate skeletal muscle fibers, and a decrease in sensitivity to or the availability of ... Disuse of the muscles, such as when muscle tissue is immobilized for even a few days of unuse - ... accompanied by a smaller number and size of the muscle cells as well as lower protein content. In humans, prolonged periods of ...
PDLIM1 - ويكيبيديا، الموسوعة الحرة
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... cell-cell adhesion. • positive regulation of nucleic acid-templated transcription. • heart development. • actin cytoskeleton ... cadherin binding involved in cell-cell adhesion. • actin binding. • muscle alpha-actinin binding. ... Pitx2 pathway mediating cell-type-specific proliferation during development.". Cell. 111 (5): 673-85. PMID 12464179. doi: ...
Activated carbon
The carbon is either mixed with the solution then filtered off or immobilized in a filter. ... to higher temperatures and either combusted to do work or in the case of hydrogen gas extracted for use in a hydrogen fuel cell ...
TATA box
"Molecular Cell. 68 (1): 130-143.e5. doi:10.1016/j.molcel.2017.08.016. PMC 5632562. PMID 28918903.. ... The end result is to immobilize the TATA-binding protein (TBP) on DNA in order to down-regulate transcription initiation. ... In specific cell types or on specific promoters TBP can be replaced by one of several TBP-related factors (TRF1 in Drosophila, ... Compounds that trap the protein-DNA intermediate could result in it being toxic to the cell once they encounter a DNA ...
Biofuel
Syngas may be burned directly in internal combustion engines, turbines or high-temperature fuel cells.[27] The wood gas ... "Bioconversion of corn straw to ethanol by cellulolytic yeasts immobilized in Mucuna urens matrix". Journal of King Saud ... Electricity from wood through the combination of gasification and solid oxide fuel cells, Ph.D. Thesis by Florian Nagel, Swiss ...
Pepper spray
The Scoville rating of these sprays are 500,000 (sabre MK9 HVS unit) and 2,000,000 (Sabre, cell buster fog delivery). This was ... The definition under regulation states "any device designed to be used for the purpose of injuring, immobilizing or otherwise ... immobilizing or otherwise incapacitating any person" is a prohibited weapon.[47] ...
Pasteur Institute
The phagocytosis theory is based on the notion that phagocytes are cells that have the power to englobe foreign bodies - and ... was immobilized by the antiquated mechanical equipment. ... human vaccine obtained by genetic engineering from animal cells ...
Phimosis
In this condition, the glans is swollen and painful, and the foreskin is immobilized by the swelling in a partially retracted ... Skin that is under tension expands by growing additional cells.. Surgical[edit]. ...
Vitamin C
... distributes readily in high concentrations into immune cells, has antimicrobial and natural killer cell activities, ... and is partially immobilized. In advanced scurvy there are open, suppurating wounds and loss of teeth and, eventually, death. ... Cell. 132 (6): 1039-48. doi:10.1016/j.cell.2008.01.042. PMID 18358815. Lay summary - Science Daily (March 21, 2008).. ... Plant and Cell Physiology. 45 (9): 1271-1279. doi:10.1093/pcp/pch152. PMID 15509850.. ...
Intravenous therapy
An arm board is recommended for immobilizing the extremity for cannulation of the hand, the foot or the antecubital fossa in ... People with sickle-cell disease may require frequent blood transfusions. Early blood transfusions consisted of whole blood, but ...
Lektiny - Wikipedie
a b c „Exposure of {alpha}2,6-sialylated lactosaminic chains marks apoptotic and necrotic death in different cell types"; ... Immobilized Lotus tetragonolobus agglutinin binds oligosaccharides containing the Lex determinant „;Liying Yan, Patricia P ... Tateno H, Uchiyama N, Kuno A, Togayachi A, Sato T, Narimatsu H, Hirabayashi J."A novel strategy for mammalian cell surface ... "O-acetyl sialic acid binding lectin as a probe for detection of subtle change on cell surface induced during acute ...
Meir Wilchek
In 1975, they applied drug-conjugated antibodies for the targeted delivery of cytotoxic compounds to cancer cells.[10] They ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... or immunotoxins is a biorecognition-based approach to selectively deliver a cytotoxic drug or toxin to a specific target cell. ... as exemplified by the removal of heme peptides from blood using immobilized human serum albumin, thus laying the grounds for ...
Anterior cruciate ligament reconstruction
In the case of mesenchymal stem cells, these cell types include osteoblasts (bone cells), adipocytes (fat cells), and ... Therefore, a brace is often used to immobilize the knee for one to two weeks. Evidence suggests that the hamstring tendon graft ... Stem cell treatment[edit]. Autologous stem-cell transplantation using mesenchymal stem cells (MSCs) has been used to improve ... Ligament cells differ in size, respond to different cues in the cell environment, and express different cell surface markers, ...
ATXN2L - ويكيبيديا، الموسوعة الحرة
2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell. 125 (4): 801-14. PMID 16713569. doi:10.1016/j.cell.2006.03.032. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم ... Cell. 18 (4): 1385-96. PMC 1838996. . PMID 17392519. doi:10.1091/mbc.E06-12-1120. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط , ... doi:10.1016/j.cell.2006.09.026. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم تجاهله (مساعدة); الوسيط ,العنوان=. تم ...
DNA sequencing
50,000 per SMRT cell, or 500-1000 megabases[69][70]. 30 minutes to 4 hours[71]. $0.13-$0.60. Fast. Detects 4mC, 5mC, 6mA.[72]. ... A non-radioactive method for transferring the DNA molecules of sequencing reaction mixtures onto an immobilizing matrix during ... ruled that individuals have no property rights to discarded cells or any profits made using these cells (for instance, as a ... In this method, DNA molecules and primers are first attached on a slide or flow cell and amplified with polymerase so that ...
List of Attack on Titan characters
When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Gerald was seen in a prison cell.. Roderich (ロー ... As a small Titan begins eating her mother alive, Kaya is immobilized by a state of shock. She is rescued when Sasha (who is ... When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Roderich was seen in a prison cell.. Civilians[ ... When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Aurille was seen in a prison cell.. Deltoff ( ...
Smart polymer
This technique has been used to control ligand and cell binding activity, based on a variety of triggers including temperature ... The dressing design presents proprietary super-absorbent synthetic smart polymers immobilized in the 3-dimensional fiber matrix ... reactions using smart polymers is to prepare recombinant proteins with built-in polymer binding sites close to ligand or cell ...
Ion chromatography
... where membranes are cut into squares and immobilized. A more recent method involved the use of live cells that are attached to ... It works because ions from the mobile phase interact with the immobilized ions on the stationary phase, thus "shielding" the ... Ionizable molecules that are to be purified compete with these exchangeable counterions for binding to the immobilized charges ... Among the most common immobilized charged groups used are trimethylaminoethyl (TAM), triethylaminoethyl (TEAE), diethyl-2- ...
AMPA receptor
"Cell. 135 (3): 535-48. doi:10.1016/j.cell.2008.09.057. PMC 2585749 . PMID 18984164.. ... stargazin is essential for immobilizing AMPA receptors in the PSD by interacting with PSD-95.[44] PSD-95 stabilizes AMPA ... Shepherd JD, Huganir RL (2007). "The cell biology of synaptic plasticity: AMPA receptor trafficking". Annu. Rev. Cell Dev. Biol ... doi:10.1016/j.cell.2006.08.037. PMID 17018279.. *^ Kim DY, Kim SH, Choi HB, Min C, Gwag BJ (2001). "High abundance of GluR1 ...
Lipase, a enciclopedia libre
"Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support ... Spiegel S, Foster D, and R Kolesnick (1996). "Signal transduction through lipid second messengers". Current Opinion in Cell ...
Snake
"Cell. 167 (3): 633-642.e11. doi:10.1016/j.cell.2016.09.028. PMC 5484524. PMID 27768887.. ... Cobras, vipers, and closely related species use venom to immobilize, injure or kill their prey. The venom is modified saliva, ... The thymus is located in fatty tissue above the heart and is responsible for the generation of immune cells in the blood. The ...
European mantis
Caught prey gets trapped between the spikes on femur and tibia and is now immobilized. The mantis then starts eating the still ... The eyes of M. religiosa are apposition eyes with eight types of photoreceptor cells and therefore best adapted for daylight ... Yager, David; Hoy, Ronald (December 1987). "The midline metathoracic ear of the praying mantis, Mantis religiosa". Cell and ...
SNP array
An array containing immobilized allele-specific oligonucleotide (ASO) probes.. *Fragmented nucleic acid sequences of target, ... DNA copy number profile for the T47D breast cancer cell line (Affymetrix SNP Array) ... LOH profile for the T47D breast cancer cell line (Affymetrix SNP Array) ... "Integrative genomic deconvolution of rheumatoid arthritis GWAS loci into gene and cell type associations". Genome Biology. 17 ( ...
Tendon
There is a three-dimensional network of cell processes associated with collagen in the tendon. The cells communicate with each ... In rabbits, collagen fascicles that are immobilized have shown decreased tensile strength, and immobilization also results in ... Mechanical forces can be transmitted by focal adhesion sites, integrins, and cell-cell junctions. Changes in the actin ... "Tendon cells in vivo form a three dimensional network of cell processes linked by gap junctions". Journal of Anatomy. 189 (Pt 3 ...
Immunotherapy
"Adoptive immunotherapy of lung cancer with immobilized anti-TCRgammadelta antibody-expanded human gammadelta T-cells in ... Immune effector cells such as lymphocytes, macrophages, dendritic cells, natural killer cells (NK Cell), cytotoxic T ... CD4+ helper T cells, cytotoxic CD8+ T cells and B cells). This initiates a cytotoxic response against tumor cells expressing ... T-cell adoptive transfer[edit]. Adoptive cell transfer in vitro cultivates autologous, extracted T cells for later transfusion. ...
Venous thrombosis
Red blood cells and fibrin are the main components of venous thrombi,[6] and the thrombi appear to attach to the blood vessel ... In adults who have had their lower leg casted, braced, or otherwise immobilized for more than a week, LMWH may decrease the ... and this state is linked to the activation of white blood cells (leukocytes) and the endothelium. Specifically, the two ...
Chemical compound microarray
The first form is to covalently immobilize the organic compounds on the solid surface with diverse linking techniques; this ... also been directed towards studying the surface chemistry of these arrays to determine which surface chemistries control cell ...
Cat anatomy
This behavior also extends into adulthood, when a male will grab the female by the scruff to immobilize her while he mounts, ... This depolarization sensitizes muscle cells so they are more likely to contract. The stomach is not only a muscular structure, ...
Concanavalina A, a enciclopedia libre
... a model for a quantitative study of cell adhesion". J Cell Sci 56: 21-48. PMID 7166565.. ... GE Healthcare Life Sciences, Immobilized lectin. *↑ Min W, Dunn AJ, Jones DH (1992). "Non-glycosylated recombinant pro- ... "J Cell Biol 65 (1): 123-146. PMC 2111157. PMID 1092699. doi:10.1083/jcb.65.1.123.. ... "J Cell Biol 59 (1): 134-142. PMC 2110924. PMID 4201706. doi:10.1083/jcb.59.1.134.. ...
Patent US4600692 - Immobilized cells for preparing phenylalanine - Google Patents
The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free ... The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also ... for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having ... as well as other forms of immobilized cells, provided the immobilized cells have transaminase activity. According to the ...
Immobilized Cells: Basics and Applications, Volume 11 - 1st Edition
Purchase Immobilized Cells: Basics and Applications, Volume 11 - 1st Edition. Print Book & E-Book. ISBN 9780444819840, ... Immobilized-cell reactors. Scale up of immobilized-cell reactors (J.J. Heijnen). PEGASUS: innovative biological nitrogen ... Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). Immobilized cell technology in food processing (C. ... Immobilized-cell growth: diffusion limitation in expanding micro-colonies (R.H. Wijffels et al.). Competition and cooperation ...
Immobilized Cell Technology - Wikipedia
Immobilized cell technology is a method of air filtration and purification that uses whole cell immobilization. It is a process ... Oxidation through immobilized cell technology is 12 times more efficient than natural oxidation is under the same conditions. ... As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that ... The key difference between traditional air filtration systems and those that employ immobilized cell technology is that the ...
Immobilized whole cell - Wikipedia
... in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required ... The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is ... However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. Zaushitsyna, O.; ... Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience. This is only ...
Immobilized culture and transfection microarray of non-adherent cells.
... but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. Herein ... Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, ... Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the ... Previous Document: Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon .... ...
JFB | Free Full-Text | Smooth Muscle Cell Functionality on Collagen Immobilized Polycaprolactone Nanowire Surfaces
... as well as cell elongation, while collagen immobilized surfaces greatly affect cell differentiation. ... are upregulated on collagen immobilized surfaces. These results suggest that nanotopography affects cell adhesion, ... The nanowire surfaces were fabricated from polycaprolactone and were immobilized with collagen. The objective of this study is ... The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that ...
Article Metrics - Molecular weight of surface immobilized hyaluronic acid influences CD44-mediated binding of gastric cancer...
Optimizing Immobilized Enzyme Performance in Cell-Free Environments to Produce Liquid Fuels (Technical Report) | SciTech Connect
... an alternate simplified biofuel production scheme was tested based on a cell-free immobilized enzyme system. Using this cell ... 09 BIOMASS FUELS; biofuel; cell-free; enzymes; immobilized; isobutanol; pervaporation; brush membranes.. ... Title: Optimizing Immobilized Enzyme Performance in Cell-Free Environments to Produce Liquid Fuels ... Technical Report: Optimizing Immobilized Enzyme Performance in Cell-Free Environments to Produce Liquid Fuels ...
High Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum | SpringerLink
The use of immobilized cells o. N. tabacum in sodium alginate gel much improved the yield of the products; the glycosylation of ... Glycosylation β-thujaplicin β-glucoside β-gentiobiosid Nicotiana tabacum immobilized cells This is a preview of subscription ... High Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum. ... were obtained through the biotransformation with immobilized cells in the medium without iron ions. In comparison with the case ...
Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads.
The fermentation capacity of the immobilized yeast cells was found to be practically independent of the hydrogen ion ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. The fermentation capacity of the ... The fermentation capacity was maximal at 30 degrees C. The immobilized yeast cells were used for continuous production of ...
Adrenaline release immobilizes immune cell responses | 2021-04-30 | BioWorld
... that the neurotransmitter noradrenaline produced by the sympathetic nervous system causes a dramatic paralysis of immune cell ... Using advanced intravital microscopy to visualize immune cell movement within the tissues, investigators at the Peter Doherty ... Using advanced intravital microscopy to visualize immune cell movement within the tissues, investigators at the Peter Doherty ... that the neurotransmitter noradrenaline produced by the sympathetic nervous system causes a dramatic paralysis of immune cell ...
Probiotic Sour Milk Production Using Cells Immobilized On Wheat Bran | 17152
Probiotic sour milk production using cells immobilized on wheat bran. 5th World Congress on Biotechnology. A Terpou, M ... Compared to a sour milk product produced with free cells, the batch sour milk production is more costly effective since the ... The results of the present study demonstrated the successful use of freeze dried lactobacilli immobilized on wheat bran for ... Biology JournalBiomaterial journalBiotechnology JournalCell Science JournalChromosome Abnormalities JournalEvolutionary Biology ...
Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(iii) complexes - Chemical...
Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes J. Cao, C. ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes ... Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ...
Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells. |...
Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells.. [ ... Cell uptake studies showed significant uptake of HA-coated nanosensors in HeLa cells and no uptake under the same conditions ... It is not clear if NP size, surface chemistry, and/or targeting of cell surface receptors changes the intracellular fate of NPs ... The pH distribution profile in cells was measured for nanosensors with HA, cationic, and noncharged NP surface coatings giving ...
Preparation of optically active β-hydroxy-α-amino acid by immobilized Escherichia coli cells with serine hydroxymethyl...
... and 0.1 g/ml of immobilized SHMT cells at pH 7.5 and 45°C. Under these conditions, the immobilized cells were continuously used ... Preparation of optically active β-hydroxy-α-amino acid by immobilized Escherichia coli cells with serine hydroxymethyl ... The immobilized cells exhibited favourable operational stability.. Keywords. d-threo-Amino acid Enzymatic resolution ... serine were used in the resolution process catalyzed by immobilized Escherichia coli cells with SHMT activity. It was observed ...
Marine Drugs | Free Full-Text | Biotransformation and Detoxification of Xylidine Orange Dye Using Immobilized Cells of Marine...
Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-( ... the cells were immobilized in sodium alginate beads since immobilized cells had several advantages over suspended cells [16]. ... Such immobilized and stored cells can regain their viability upto several months (1-3 months) which can be an important ... The method of using L. sphaericus D3 cells immobilized in natural gel alginate beads was stable for longer periods, and with ...
Immobilized chemoattractant peptides mediate adhesion and distinct calcium-dependent cell signaling in human neutrophils
... ... The reference peptide generated no such activation, and the cells maintained a more spherical morphology, suggesting that we ... Chemotaxis is the stimulated directional migration of cells in response to chemotactic factors, manifested for instance during ... To accomplish defined circumstances for recruitment and activation of cells, we fabricated semitransparent gold-coated glass ...
Frontiers | Simultaneous Alcoholic and Malolactic Fermentations by Saccharomyces cerevisiae and Oenococcus oeni Cells Co...
The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized-cell system, that we here describe for the first time in our knowledge, offers many advantages over ... In recent years, the use of immobilized cell systems has been investigated, with interesting results, for the production of ...
A Rapid Technique for the Visualization of Live Immobilized Yeast Cells | Protocol (Translated to Swedish)
Zawadzki, K., Broach, J. A Rapid Technique for the Visualization of Live Immobilized Yeast Cells. J. Vis. Exp. (1), e84, doi: ... Cell 2006.. Protocol. * Immobiliserade celler för tidsförloppet epifluorescence mikroskopi. Observera att detta protokoll är ... Min mikroskopi visade att det inte finns något samband mellan den epigenetiska tillstånd HML och HMR i en enskild cell. sir1 ... Detta är en snabb teknik som immobilizes jästceller samtidigt tillåta tillväxt. Vi har följt jäst tillväxt för upp till tio ...
Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells...
Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ...
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation
... hydrogel platform to evaluate the effects of substrate stiffness and cell adhesive ligands on VEC phenotype and expression of ... and 20 kDa were polymerized into platforms of different rigidities and thiol-modified cell adhesive peptides were covalently ... have unique phenotypic responses relative to other types of vascular endothelial cells and have highly sensitive hemostatic ... Endothelial cells Is the Subject Area "Endothelial cells" applicable to this article? Yes. No. ...
Human Prekallikrein & Kallikrein Clone 10G3-42 Immobilized mAb | Cell Sciences
Formation of a Mast Cell Synapse: FcεRI Membrane Dynamics upon Binding Mobile or Immobilized Ligands on Surfaces | The Journal...
Evidence for direct contacts between mast cells and other immune cells (21-23) indicates that mast cell synapses may play a ... Given recent evidence for mast cells as bona fide immune modulatory cells (21-23), synapses between mast cells and other ... 3). TIRF imaging of GFP-actin cells indicated that actin became more concentrated at the cell periphery (ring-like) after cells ... and linker for activation of T cells, were also analyzed. Stimulation of mast cells with immobilized polyvalent ligand resulted ...
Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier...
BRIN-BD11 cells immobilized on cytodex-1 or cultispher-Gout-performed single cell suspensions and pseudoislets in terms ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ... Similar growth profiles were recorded for eachmicrocarrier with densities of 4.4×105±0.3 cells/ml and4.2×105±0.2 cells/ml ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ...
Synergistic enhancement of cytotoxicity against cancer cells by incorporation of rectorite into the paclitaxel immobilized...
Synergistic enhancement of cytotoxicity against cancer cells by incorporation of rectorite into the paclitaxel immobilized ... Batteries Fine Art Materials Fuel Cells Glass Investment Grade Metals Jewelry & Fashion Lasers Lighting Medical Devices Museums ... Supercapacitor Materials Catalysts COVID-19 Prevention Materials Dental Materials Electronics Materials Fuel Cell Materials ... the cytotoxicity of human gastric adenocarcinoma SGC7901 cells grown on the mats was enhanced, which suggested that rectorite ...
Malic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe | American Journal of Enology and Viticulture
All experiments were carried out using dry immobilized yeast cells instead of wet immobilized yeast cells as in previous works ... the high malate fermentation activity of the immobilized yeast cells was similar to that of the non-immobilized cells. ... Alginate bead quality tests showed that immobilized cells could be recycled up to five times without liberation of yeast cells ... Malic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe Sofía Silva, Felipe Ramón-Portugal, Patricia ...
Bioactive compounds immobilized on Ti and TiNbHf: AFM-based investigations of biofunctionalization efficiency and cell adhesion
... cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell ... In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment ... Our results show that bioactive moieties, particularly fibronectin fragment III8-10, improve cell adhesion on titanium and ... Implant materials require optimal biointegration, including strong and stable cell-material interactions from the early stages ...
Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re...
Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ...
Carrier-bound or immobilized enzyme or microbial cell; carrier-bound or immobilized cell; preparation thereof > Enzyme or...
... carrier-bound or immobilized cell; preparation thereof > Enzyme or microbial cell is immobilized on or in an organic carrier > ... Method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell ... Name: Chemistry: molecular biology and microbiology > Carrier-bound or immobilized enzyme or microbial cell; ... Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode. Jun. 9, 2009. ...
Immobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide Detection | Science...
This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and ... The immobilized cells were used as a bio-component for spectrophotometric detection of OP compounds, with MP as a ... Scanning electron microscope and cell count analyses revealed highly dense, rod shaped bacterial cells stably attached to the ... Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading ...
FermentationEnzymesBiocatalystAffinity chromatographyAbstractATCCEnzymeImmobilizationReactorYeastBacillus2016SaccharomycesBiocatalystsAdhesionSubstratesCharacterization of immobilizedProduction by immobilizedActivity of immobilizedPseudomonasBeadsProliferationMicrobialCovalently immobilizedSurfacesSubstrateBatchViabilityDifferentiationReceptorsMembranesProteinsStrainsLigandBacteriaMembrane bioreactorDegradationCollagenPeptideEnzymaticHyaluronicEthanol productionLigandsPeptidesSurfaceMammalianMicroorganismStrainStabilityFreeWastewaterVitroCelluloseCulturesAlginateBasics and ApplicationsKineticCatalystsSodiumEfficiencyEnrichmentGrowthEndothelial cellsReaction
Fermentation19
- The fermentation capacity of the immobilized yeast cells was found to be practically independent of the hydrogen ion concentration between pH 3.1 and 6.25. (biomedsearch.com)
- The fermentation capacity was maximal at 30 degrees C. The immobilized yeast cells were used for continuous production of ethanol in a fluidized-bead reactor. (biomedsearch.com)
- Co-immobilization of S. cerevisiae and O. oeni allowed to perform an efficient fermentation process, producing low volatile acidity levels and ethanol and glycerol concentrations comparable with those obtained by cell sequential inoculum and co-inoculum of yeast and bacteria cells in free form. (frontiersin.org)
- The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of cell metabolic activities. (frontiersin.org)
- A maximum of ethanol production (17.83 g/L, yield 0.44 g/g and 20.45 g/L, yield 0.47 g/g) was observed with free and immobilized cells of P. stipitis respectively in batch fermentation conditions. (degruyter.com)
- Immobilized cells were then removed from the fermentation tank and the must was re-inoculated with a commercial strain of Saccharomyces cerevisiae to achieve alcoholic fermentation. (ajevonline.org)
- Experiments showed that in terms of kinetics, the high malate fermentation activity of the immobilized yeast cells was similar to that of the non-immobilized cells. (ajevonline.org)
- Oenococcus oeni cells immobilized on delignified cellulosic material for malolactic fermentation of wine. (semanticscholar.org)
- Oenococcus oeni ATCC 23279 cells immobilized on delignified cellulosic material (DCM) were used for malolactic fermentation (MLF). (semanticscholar.org)
- The aim of this research was to study how the yeast cell immobilization technique influences the growth and fermentation profiles of Kluyveromyces marxianus cultivated on apple/chokeberry and apple/cranberry pomaces. (comu.edu.tr)
- In this work Lactobacillus casei ATCC 393 cells immobilized on delignified cellulosic material (DCM) were used for malolactic fermentation (MLF) of wine. (ulster.ac.uk)
- Wine was produced using yeast cells immobilized on DCM at 20 degrees C, and after alcoholic fermentation, MLF at 27 degrees C followed using immobilized L. casei ATCC 393 cells. (ulster.ac.uk)
- In this study, Box-Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. (springeropen.com)
- Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. (springeropen.com)
- Immobilized cell fermentation has attracted great attention in the fields of scientific research and industry. (springeropen.com)
- Compared with free cell fermentation, immobilized cell fermentation has many advantages, such as higher cell density in the fermenter, higher yield and production rate during fermentation process, high biological activity maintained for a long fermentation period, and the convenience of product recovery (Kumar et al. (springeropen.com)
- 2013 ). In addition, immobilized cells with excellent reusability can be easily separated from the fermentation medium, so they can be used in repeated batch fermentations to reduce the time and cost of cell culture (Djukić-Vuković et al. (springeropen.com)
- A biocatalyst was prepared by immobilizing a commercial Saccharomyces cerevisiae strain (baker's yeast) on orange peel pieces for use in alcoholic fermentation and for fermented food applications. (elsevier.com)
- Cell immobilization was shown by electron microscopy and by the efficiency of the immobilized biocatalyst for alcoholic fermentation of various carbohydrate substrates (glucose, molasses, raisin extracts) and at various temperatures (30-15 degrees C). Fermentation times in all cases were low (5-15 h) and ethanol productivities were high (av. 150.6 g/ld) showing good operational stability of the biocatalyst and suitability for commercial applications. (elsevier.com)
Enzymes16
- However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. (wikipedia.org)
- To increase stability and conversion efficiency with easy separations, the first two enzymes were immobilized onto methacrylate resin. (osti.gov)
- Immobilized enzymes from Geotrichum spp. (semanticscholar.org)
- An enzymatic biofuel cell is a specific type of fuel cell that uses enzymes as a catalyst to oxidize its fuel, rather than precious metals. (wikipedia.org)
- This offers a couple of advantages for enzymatic biofuel cells: Enzymes are relatively easy to mass-produce and so benefit from economies of scale , whereas precious metals must be mined and so have an inelastic supply . (wikipedia.org)
- if not immobilized, the enzymes will diffuse into the cell's fuel and most of the liberated electrons will not reach the electrodes, compromising its effectiveness. (wikipedia.org)
- The former technique is possible only with certain types of enzymes whose activation sites are close to the enzyme's surface, but doing so presents fewer toxicity risks for fuel cells intended to be used inside the human body. (wikipedia.org)
- [1] Research on using enzymes directly for oxidation in biofuel cells began in the early 1960s, with the first enzymatic biofuel cell being produced in 1964. (wikipedia.org)
- While the early cells did successfully produce electricity , there was difficulty in transporting the electrons liberated from the glucose fuel to the fuel cell's electrode and further difficulties in keeping the system stable enough to produce electricity at all due to the enzymes' tendency to move away from where they needed to be in order for the fuel cell to function. (wikipedia.org)
- Increased quantities of synthesized enzymes were proportional to the increment of initial biomass present in beads, ant to a lesser degree, depended on quantities of immobilized inoculum. (olsztyn.pl)
- In repeated batch culture, the bacteria immobilized in alginate were able to synthesize 32% more enzymes than those immobilized in chitosane. (olsztyn.pl)
- Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. (bioportfolio.com)
- Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields. (bioportfolio.com)
- In this work, using benzydamine as a model drug, two approaches were tested and optimized for the synthesis of benzydamine N -oxide: an enzyme immobilization system using two HisTrap HP columns connected in series carrying His 6 -GDH and His 6 -FMO3 enzymes respectively, and a two-strain-mixed-culture strategy using cell suspensions of E. coli JM109 (containing a FMO3 gene) and BL21 (containing a GDH gene). (biomedcentral.com)
- This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. (springer.com)
- Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field. (springer.com)
Biocatalyst4
- Compared to a sour milk product produced with free cells, the batch sour milk production is more costly effective since the biocatalyst can be used several times. (omicsonline.org)
- The immobilized cell-based transaminase biocatalyst proved to be durable and easy-to-use in kinetic resolution of four racemic amines 1a-d. (elsevier.com)
- As the repeated MLF batches proceeded, the MLF activity of the immobilized biocatalyst was reduced. (ulster.ac.uk)
- The objective of this study is to determine optimum pH and temperature of hydrolysis process using lipase of whole-cell biocatalyst Mucor circinelloides immobilized in Poly-Urethane Foam (PUF). (ubaya.ac.id)
Affinity chromatography5
- ARTICLE{Nühse03largescaleanalysis, author = {Thomas S. Nühse and Allan Stensballe and Ole N. Jensen and Scott C. Peck}, title = {Largescale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry}, journal = {Mol. (psu.edu)
- To date, immobilized metal ion affinity chromatography (IMAC) for phos-phopeptides has shown great promise for large-scale studies, but has a reputation for poor specificity. (psu.edu)
- Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity. (nih.gov)
- We carried out phosphoproteomic analysis of rat inner medullary collecting duct cells by using a combination of phosphopeptide enrichment by immobilized metal affinity chromatography and phosphorylation site identification by liquid chromatography-mass spectrometry n neutral loss scanning. (pnas.org)
- Recent studies have introduced two new innovations, namely, immobilized metal affinity chromatography (IMAC) ( 1 - 3 ) and liquid chromatography-mass spectrometry (LC-MS) 3 neutral loss scanning ( 1 - 4 ), to increase the efficiency of phosphopeptide identification. (pnas.org)
Abstract1
- abstract = "Immobilization of Candida rugosa cells on a solid support for extracellular lipase production has been explored. (uab.cat)
ATCC1
- The aim of the present study was to evaluate the production of sour milk fermented with the use of the probiotic microorganism Lactobacillus casei ATCC 393 immobilized on wheat bran. (omicsonline.org)
Enzyme14
- The immobilized whole cell system is an alternative to enzyme immobilization. (wikipedia.org)
- Unlike enzyme immobilization, where the enzyme is attached to a solid support (such as calcium alginate or activated PVA or activated PEI), in immobilized whole cell systems, the target cell is immobilized. (wikipedia.org)
- Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. (google.ca)
- 14. A composition according to claim 13 wherein the enzyme is present in the form of whole cells. (google.ca)
- 15. A composition according to claim 13 wherein the enzyme is present in the form of cells which have been ruptured or permeabilized. (google.ca)
- To overcome these challenges, an alternate simplified biofuel production scheme was tested based on a cell-free immobilized enzyme system. (osti.gov)
- Subject matter wherein the enzyme or microbial cell is bonded to the polymeric carrier through an intermediate compound which attaches to both the enzyme and the carrier. (patentgenius.com)
- [2] These difficulties led to an abandonment by biofuel cell researchers of the enzyme-catalyst model for nearly three decades in favor of the more conventional metal catalysts (principally platinum), which are used in most fuel cells. (wikipedia.org)
- However, cell lysis is one of the major drawbacks during the excretion of recombinant enzyme when using Escherichia coli (E. coli) as a host. (ump.edu.my)
- Cell immobilization is a promising solution for the enhancement of enzyme excretion with reduction of cell lysis. (ump.edu.my)
- In the present study, a recombinant E. coli was immobilized using hollow fiber membrane to improve the enzyme excretion, cell viability and plasmid stability. (ump.edu.my)
- In this work, using benzydamine as a model drug, two easy-to-perform approaches (whole cell catalysis and enzyme immobilization) were investigated for the synthesis of FMO3-generated drug metabolites. (biomedcentral.com)
- Kinetic constants for the immobilized enzyme have been identified. (inrae.fr)
- Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. (bvsalud.org)
Immobilization17
- Immobilized cell technology is a method of air filtration and purification that uses whole cell immobilization. (wikipedia.org)
- whole cell immobilization may be used for convenience. (wikipedia.org)
- Porous silicone rubber as an immobilization matrix for microbial and mammalian cells: natural immobilization of a mass transfer limited culture environment (A.J. Knights).Local mass transfer coefficients in bacterial biofilms using fluorescence recovery after photobleaching (FRAP) biofilms (J.D. Bryers, F. Drummond). (elsevier.com)
- Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. (biomedsearch.com)
- Complexes 2 and 4 , containing reactive chloromethyl groups for mitochondrial fixation, show much higher cytotoxicity than complexes 1 and 3 without mitochondria-immobilization properties against the cancer cells screened. (rsc.org)
- Immobilization of yeast cells on sorghum stalks were confirmed by scanning electron microscopy (SEM). (degruyter.com)
- The adhesion of mouse STO fibroblast cells was accelerated by the immobilization of polyallylamine. (semanticscholar.org)
- The adhesion and growth of the cells were both markedly accelerated by the co-immobilization of the polyallylamine and insulin. (semanticscholar.org)
- Further biodegradation studies at phenol concentrations of 2,000 and 3,500 mg/L were also performed to evaluate the effectiveness of cell immobilization for delaying the effects of substrate inhibition. (nus.edu.sg)
- Immobilization of Raji cells on surface coated with anti-lymphocyte globulin (ALG) at low cell densities lead to the synthesis of Epstein-Barr virus (EBV) early antigen (EA) in up to 5% of the cells. (uni-regensburg.de)
- Hence, the immobilization of E. coli on hollow fiber membrane proved to be valuable for the excretion of recombinant proteins in E. coli with high cell stability. (ump.edu.my)
- cells were immobilized on a macroporous polystyrene particle matrix that provides a promising novel support for cell immobilization. (eugris.info)
- Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. (frontiersin.org)
- 2017 ). It has been reported that SA-PVA hydrogel for cell encapsulation has significant advantages over cell immobilization using a single material. (springeropen.com)
- Therefore, SA-PVA hydrogel can be regarded as a proper material for cell immobilization (Zhan et al. (springeropen.com)
- The project encompasses complex solution of a bioremediation technology:microbial degradation of organic pollutants,whole cell immobilization, reactor design and environmental engineering enabling tailor-made bioremedial actions. (eurekanetwork.org)
- Purpose: This study examined the effects of the immobilization of Arg-Gly-Asp (RGD) peptide (CAAALLLKERGDSK) on anodized titanium (Ti) via chemical grafting or physical adsorption methods on cell adhesion and osteoblast differentiation. (elsevier.com)
Reactor10
- Sucrose hydrolysis was studied in a continuously immobilized cell reactor. (ije.ir)
- This fact demonstrates that the reactor system is limited in terms of organic substrate dispersion and direct contact with cells. (uab.cat)
- The Advanced 'Immobilized Cell Reactor' technology performs at a credible level for the removal of organics estimated as BOD and COD from wastewater generated in garment leather manufacturing industry. (mirchmasalatv.com)
- The wastewater treated using the immobilized cell reactor supported the growth of vegetative plants and aquatic bred animals. (mirchmasalatv.com)
- The immobilized cell reactor treated wastewater met the discharging standards reused within the industry or used for irrigation purposes. (mirchmasalatv.com)
- The suggested treatment technology for the treatment of wastewater was anaerobic treatment followed by Advanced 'Immobilized Cell Reactor' treatment. (mirchmasalatv.com)
- Biotrans- formation has been carried out in a fluidized bed reactor specially designed to obtain good contact conditions between cells entrapped into small calcium alginate beads (2-3 mm) and low-concentration acrylamide so- lutions (10~40 g 9 1-1). (inrae.fr)
- Advanced 'Immobilized Cell Reactor' technology for treatment of wastewater. (speakerdeck.com)
- Advanced 'Immobilized Cell Reactor' technology applied to leather industry. (speakerdeck.com)
- Advanced 'Immobilized Cell Reactor' technology applied to textile industry. (speakerdeck.com)
Yeast9
- Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads. (biomedsearch.com)
- Immobilized cells of Schizosaccharomyces pombe yeast were used for total or partial deacidification of grape must. (ajevonline.org)
- All experiments were carried out using dry immobilized yeast cells instead of wet immobilized yeast cells as in previous works. (ajevonline.org)
- Deacidification activity of immobilized yeast cells was maintained for at least 20 months after their encapsulation, at which point they were stored at 4°C. Alginate bead quality tests showed that immobilized cells could be recycled up to five times without liberation of yeast cells into wine must. (ajevonline.org)
- Sucrose Inversion by Saccharomyces Yeast Cells Immobilized on to Corn Grits', International Journal of Engineering , 2(3), pp. 84-87. (ije.ir)
- Culture media with fruit waste produced good growth of free as well as immobilized yeast cells. (comu.edu.tr)
- The most varied aroma profiles were noted for immobilized yeast cultivated on apple/chokeberry pomace. (comu.edu.tr)
- Quantification of phosphopeptides in this setting has been challenging and has been successful so far in cultured cells ( 5 ) and yeast ( 6 ) but not in native mammalian cells and tissues. (pnas.org)
- All fermenta- tions with immobilized yeast cells had a shorter lag phase and faster utilization of sugars and ethanol production than those fermented with suspended cells. (vtt.fi)
Bacillus3
- In addition, the production of biosurfactants by immobilized cells of Bacillus subtilis was well studied [13, 14]. (thefreedictionary.com)
- The aim of the study was to determine optimal conditions for amylases biosynthesis by immobilized Bacillus polymyxa B-20 cells. (olsztyn.pl)
- Hydrogen peroxide degradation by immobilized cells of alkaliphilic Bacillus halodurans. (ac.ke)
20161
- 2016. Nanoscopy of bacterial cells immobilized by holographic optical tweezers. (uni-bielefeld.de)
Saccharomyces2
- Limitations on biofuel production using cell culture (Escherichia coli, Clostridium, Saccharomyces cerevisiae, brown microalgae, blue-green algae and others) include low product (alcohol) concentrations (≤0.2 vol%) due to feedback inhibition, instability of cells, and lack of economical product recovery processes. (osti.gov)
- Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. (biomedsearch.com)
Biocatalysts4
- Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). (elsevier.com)
- E. coli cells containing overexpressed transaminases of various selectivities and hollow silica microspheres as supporting agent were immobilized by an improved sol-gel process to produce immobilized transaminase biocatalysts with suitable stability and mechanical properties for continuous-flow applications. (elsevier.com)
- By using the most suitable immobilized transaminase biocatalysts, this study describes the first transaminase-based approach for the production of both pure enantiomers of 1-(3,4-dimethoxyphenyl)ethan-1-amine 1d. (elsevier.com)
- Wackett, LP 2021, ' Immobilized microbial cell biocatalysts: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology ', Microbial biotechnology , vol. 14, no. 2, pp. 752-753. (umn.edu)
Adhesion16
- These results suggest that nanotopography affects cell adhesion, proliferation, as well as cell elongation, while collagen immobilized surfaces greatly affect cell differentiation. (mdpi.com)
- Work in the 1980s by the McConnell and McCloskey laboratories ( 18 , 19 ) established that liposomes bearing haptens for IgE could mediate adhesion to rat basophilic leukemia 2H3 cell line (RBL-2H3) mast cells and provide lateral forces that induced both FcεRI clustering and serotonin release. (jimmunol.org)
- In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment III8-10 coated on titanium versus a novel low-modulus TiNbHf alloy. (upc.edu)
- The influence of the different bioactive coatings on the adhesion of rat mesenchymal stem cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell force spectroscopy. (upc.edu)
- Our results show that bioactive moieties, particularly fibronectin fragment III8-10, improve cell adhesion on titanium and TiNbHf and that the covalent tethering of such molecules provides the most promising strategy to biofunctionalize these materials. (upc.edu)
- Polyallylamine, which is cationic and enhances cell adhesion electrostatically (non-specifically), was co-immobilized with a cell growth factor, insulin, onto a surface-hydrolysed poly(methyl methacrylate) membrane. (semanticscholar.org)
- Specific cell-cell recognition and adhesion may involve cell surface glycoconjugates on one cell binding the complementary carbohydrate receptors on an apposing cell surface. (elsevier.com)
- This carbohydrate-directed cell adhesion may depend on cell surface carbohydrate receptors which mediate both the initial specific adhesion and complex postrecognition cellular responses. (elsevier.com)
- While the relationship of the cell adhesion demonstrated here to cell-cell recognition in vivo has yet to be determined, this well-controlled biochemical approach may reveal new information on the way in which cells analyze and respond to their immediate external environment. (elsevier.com)
- Cell adhesive peptides, GRGDS and YIGSR, were found to promote adhesion and spreading of ECs and showed a synergistic effect when both of them were used. (aminer.cn)
- In vitro studies indicate that both adhesion to epithelial cells and auto aggregation are mostly mediated by type IV pili (Carbonnelle et al , 2006 ). (embopress.org)
- The invention discloses a cell culture support which provides for the adhesion and culturing of one or more adhesive cells using a photoresist in which to provide a particular patterned design on a surface of the support. (google.com)
- Also a reagent can be added to pretreat the support surface in order to facilitate adhesion at the photoresist prior to applying the photoresist into the cell culture support. (google.com)
- Collagen specifically affects the cell adhesion rate or the morphology of the cells to be adhered to the support. (google.com)
- 3. The cell culture support of claim 1 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface. (google.com)
- Results: Cell adhesion was enhanced on the RGD-immobilized Ti substrates compared to the anodized Ti surfaces. (elsevier.com)
Substrates6
- Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface. (biomedsearch.com)
- A series of new substrates, β-phenylserine, β-(nitrophenyl) serine and β-(methylsulfonylphenyl) serine were used in the resolution process catalyzed by immobilized Escherichia coli cells with SHMT activity. (springer.com)
- This work demonstrated the potential of collagen IV-immobilized polyimide membranes as substrates for the growth of ARPE-19 cells. (tut.fi)
- Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. (elsevier.com)
- In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity. (elsevier.com)
- 05). Conclusion: RGD-immobilized Ti substrates might be effective in improving the osseointegration of dental implants. (elsevier.com)
Characterization of immobilized3
- Guidelines for the characterization of immobilized cells (R.H. Wijffels et al. ). (elsevier.com)
- Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given. (elsevier.com)
- The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. (elsevier.com)
Production by immobilized1
- Ferrer, P & Solà, C 1992, ' Lipase production by immobilized Candida rugosa cells ', Applied Microbiology and Biotechnology , vol. 37, no. 6, pp. 737-741. (uab.cat)
Activity of immobilized2
- Nitrification activity of immobilized activated sludge evaluated by respiration rate (H. Nakamura et al. ). (elsevier.com)
- The uricase activity of immobilized cells had a value of KM and [V.sub.max], respectively of 2.86 mM and 0.0018 mA. (thefreedictionary.com)
Pseudomonas5
- An immobilized-cell membrane bioreactor was fabricated to investigate degradation of phenol at high concentrations using Pseudomonas putida American Type Culture Collection 49451. (nus.edu.sg)
- Aerobic biodegradation of phenol by Immobilized Pseudomonas sp. (sciepub.com)
- Fe(II) speciation and its uptake by free and immobilized cells of Pseudomonas fluorescens from industrial waste water. (bvsalud.org)
- Studies are carried out to remove Fe(II) from wastewater using free and immobilized cells of Pseudomonas fluorescens . (bvsalud.org)
- Fe(II) uptake with Pseudomonas fluorescens is also investigated after the addition of NaCl and MgCl2 to the cells . (bvsalud.org)
Beads5
- 2. The process of claim 1 wherein the immobilized cells are coated onto beads or other particulate material. (google.ca)
- Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-(dimethylphenylazo)-2-naphthol-6-sulfonic acid sodium salt in the laboratory. (mdpi.com)
- The process was monitored by chemical and sensorial analyses and dominance of starters and cell leaking from beads were also checked. (frontiersin.org)
- PVA effectively improves the stability of immobilized cell beads with a promoted mechanical strength, while SA improves the surface properties of immobilized cell beads to keep from the tendency to agglomeration. (springeropen.com)
- In contrast, the maximum rate of hydrolysis is considerably decreased, as if only cells on the outskirts of beads were involved in the transformation. (inrae.fr)
Proliferation6
- Inhibition of smooth muscle cell (SMC) proliferation and preservation of a differentiated state are important aspects in the management, avoidance and progression of vascular diseases. (mdpi.com)
- Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. (rsc.org)
- In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell proliferation activity. (vbripress.com)
- Indirect immobilized Jagged1 significantly reduced cell proliferation, colony forming unit ability, and the number of cells in S phase. (elsevier.com)
- Ligand binding to cell surface receptors activates multiple protein tyrosine phosphorylation-mediated signaling cascades that regulate many cell biological processes, including proliferation, differentiation, migration, and cell death ( 1 - 3 ). (pnas.org)
- The level of cell proliferation was investigated via tetrazolium (XTT) assay. (elsevier.com)
Microbial3
- fuel cells that use whole cells to catalyze fuel are called microbial fuel cells ). (wikipedia.org)
- Early work with biofuel cells, which began in the early 20th century, was purely of the microbial variety. (wikipedia.org)
- Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. (bioportfolio.com)
Covalently immobilized3
- Fusion proteins of labile KdcA (fKdcA) were expressed to stabilize the covalently immobilized KdcA. (osti.gov)
- Covalently immobilized ADH exhibited long-term stability and efficient conversion of aldehyde to alcohol over multiple batch cycles without fusions. (osti.gov)
- In this work, collagen IV was covalently immobilized to the surface of polyimide membranes, with the purpose of developing scaffold materials for RPE cell culture. (tut.fi)
Surfaces12
- In this study, we have developed collagen immobilized nanostructured surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of human aortic SMCs. (mdpi.com)
- The nanowire surfaces were fabricated from polycaprolactone and were immobilized with collagen. (mdpi.com)
- The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that SMCs on nanostructured surfaces may be differentiated and slowly dividing. (mdpi.com)
- Scanning electron microscopy results reveal that SMCs on nanostructured surfaces are more elongated and that cells are interacting with the nano-features on the surface. (mdpi.com)
- After providing differentiation cues, heavy chain myosin and calponin, specific to a contractile SMC phenotype, are upregulated on collagen immobilized surfaces. (mdpi.com)
- Rat basophilic leukemia mast cells primed with fluorescent anti-DNP IgE were engaged by surfaces presenting either bilayer-incorporated, monovalent DNP-lipid (mobile ligand), or chemically cross-linked, multivalent DNP (immobilized ligand). (jimmunol.org)
- In this study, we explore the potential for mast cells to form synapses when presented with Ag on surfaces, analogous to the responses of T cells and B cells. (jimmunol.org)
- To overcome this problem, newly modified surfaces were designed and evaluated for the cell-rolling surface. (nii.ac.jp)
- Hydrophilic polymer grafting, silane coupling reagents, and SAM on Au were selected and evaluated for the cell-rolling surfaces. (nii.ac.jp)
- Such interactions have been modeled by immobilizing simple synthetic glycosides, glycoproteins, glycosaminoglycans, and glycolipids on otherwise inert plastic surfaces and incubating them with intact cells. (elsevier.com)
- We have developed a microfluidic flow cell where stepwise enzymatic digestion is performed on immobilized proteoliposomes and the resulting cleaved peptides are analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The flow cell channels consist of two parallel gold surfaces mounted face to face with a thin spacer and feature an inlet and an outlet port. (chalmers.se)
- Covalently modified surfaces showed cell attachment and cell properties comparable to the uncoated and dip-coated controls. (tut.fi)
Substrate3
- A common use of immobilized cells is in biotechnology for the bioconversion of a substrate to a particular product. (bioportfolio.com)
- The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. (elsevier.com)
- The chemical composition of each RGD-immobilized Ti substrate was examined by x-ray photoelectron spectroscopy. (elsevier.com)
Batch2
- The immobilized cells were successfully reused for BDS over seven batch cycles but the uncoated free cells could be used only once. (thefreedictionary.com)
- Moreover, in repeated-batch operations, immobilized Arthrobacter sp. (eugris.info)
Viability5
- Viability of immobilized cells: use of specific ATP levels and oxygen uptake rates (P. Gikas, A.G. Livingston). (elsevier.com)
- Cell viability began to decline on day 5 ofculture. (portlandpress.com)
- Accordingly, the following objectives were set: i) to evaluate the impact of low-intensity radiation on cell suspensions, and ii) to carry out a comparative analysis of the viability of cells immobilized in porous TiNi-based alloy and IR- and UV-irradiated. (vbripress.com)
- The data show that the extracellular environment of bone marrow, tumor and spleen cell populations affects their viability and proliferative potency in porous TiNi-based scaffolds. (vbripress.com)
- IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, tumor, and spleen cells. (vbripress.com)
Differentiation2
- However, immature myeloid cells are also an intrinsic part of the normal process of myeloid cell differentiation, and they are present in relatively small numbers in naive hosts. (jimmunol.org)
- In conclusion, indirect immobilized Jagged1 suppresses cell cycle progression and induces the odonto/osteogenic differentiation of hDPs via the canonical Notch signaling pathway. (elsevier.com)
Receptors1
- These data indicate that FcεRI cross-linking is not an obligatory step in triggering mast cell signaling and suggest that dense populations of mobile receptors are capable of initiating low-level degranulation upon ligand recognition. (jimmunol.org)
Membranes1
- In the immobilized system, it was observed that cells diffused from the membranes when phenol concentrations reached noninhibitory levels in a few experiments. (nus.edu.sg)
Proteins7
- The evidence was getting stronger that cells had evolved a fine-tuned mechanism for organizing some of their internal structure and processes through phase separation-that is, letting proteins self-assemble into structures that could perform distinct functions. (scientificamerican.com)
- To investigate the relationship between NF-κB and GM-CSF transcription in a chromatin context, we analyzed the chromatin structure of the GM-CSF gene in T cells and the role of NF-κB proteins in chromatin remodeling. (rupress.org)
- These results suggest that chromatin remodeling across the GM-CSF promoter in T cells is a result of recruitment of SWI/SNF type remodeling complexes by NF-κB proteins binding to the CD28 response region of the promoter. (rupress.org)
- Organisms use reversible phosphorylation of proteins to control many cellular processes including signal transduction, gene expression, cell cycle, cytoskeletal regulation, and apoptosis. (mcponline.org)
- Anti-TMT Resin is agarose-immobilized anti-TMT monoclonal antibody, which is effective to capture and affinity purify peptides or proteins that have been labeled using any TMT Reagent. (fishersci.com)
- Recent developments in MS have also enabled the quantitative analysis of protein phosphorylation on hundreds of proteins, information that has been correlated to cell fate in recent studies ( 4 - 6 ). (pnas.org)
- Conventional tissue markers include cell morphology and metabolism, the presence of certain enzymic activities or proteins or other molecules of biological import, the accumulation or disappearance of such molecules, etc. (google.ca)
Strains1
- These two strains showed Michaelien Kinetics in the sucrose concentration up to 0.2 M and inhibitory effect in the concentration beyond 0.2 M. These two strains were immobilized on to corn - grits by an adsorption technique. (ije.ir)
Ligand4
- Cell uptake studies showed significant uptake of HA-coated nanosensors in HeLa cells and no uptake under the same conditions without the HA targeting ligand. (sigmaaldrich.com)
- Stimulation of mast cells with immobilized polyvalent ligand resulted in typical levels of degranulation. (jimmunol.org)
- Remarkably, degranulation also followed interaction of mast cells, with bilayers presenting mobile, monovalent ligand. (jimmunol.org)
- The present study investigated the response of human dental pulp cells (hDPs) to the indirect immobilized Notch ligand Jagged1 in vitro. (elsevier.com)
Bacteria1
- The method proposed in this work is based on conversion of the waste glycerol to dihydroxyacetone in the reaction catalyzed by an immobilized cell extract with the activity of glycerol dehydrogenase, produced from Gluconobacter oxydans bacteria and it could be an effective way to utilize the waste glycerol into a valuable product. (ejbiotechnology.info)
Membrane bioreactor1
- The membrane bioreactor also showed 2.5-fold increase in the CGTase excretion (473 x 103 U/ml) with 75% reduction of cell lysis compared to shake flask culture (190 x 103 U/ml of CGTase activity). (ump.edu.my)
Degradation5
- On the other hand, cells immobilized in 25% by weight polysulfone fibers degraded phenol at concentrations above 1,000 mg/L. At an initial phenol concentration of 1,200 mg/L, phenol was fully degraded within 95 h in the immobilized system, whereas no cell growth and phenol degradation were observed in the free suspension system at 1,000 mg/L phenol. (nus.edu.sg)
- In such cases, the time taken for complete degradation was shorter with cell diffusion because suspension cells were responsible for the rapid phenol degradation. (nus.edu.sg)
- Environmental Expert.com/Microbiological degradation of pentane by immobilized cells of Arthrobacter sp. (eugris.info)
- Year: 2005 Title: Microbiological degradation of pentane by immobilized cells of Arthrobacter sp. (eugris.info)
- cells were able to maintain 85-95% pentane degradation during a 2month period. (eugris.info)
Collagen1
- The objective of this study is to reveal how SMCs interact with collagen immobilized nanostructures. (mdpi.com)
Peptide2
- Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. (semanticscholar.org)
- Materials and Methods: The RGD peptide was immobilized on the anodized Ti surface by means of physical adsorption or chemical grafting. (elsevier.com)
Enzymatic7
- The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. (degruyter.com)
- Enzymatic biofuel cells, while currently confined to research facilities, are widely prized for the promise they hold in terms of their relatively inexpensive components and fuels, as well as a potential power source for bionic implants. (wikipedia.org)
- A general diagram for an enzymatic biofuel cell using Glucose and Oxygen . (wikipedia.org)
- Enzymatic biofuel cells work on the same general principles as all fuel cells: use a catalyst to separate electrons from a parent molecule and force it to go around an electrolyte barrier through a wire to generate an electric current. (wikipedia.org)
- What makes the enzymatic biofuel cell distinct from more conventional fuel cells are the catalysts they use and the fuels that they accept. (wikipedia.org)
- [1] Because sugars and other biofuels can be grown and harvested on a massive scale, the fuel for enzymatic biofuel cells is extremely cheap and can be found in nearly any part of the world, thus making it an extraordinarily attractive option from a logistics standpoint, and even more so for those concerned with the adoption of renewable energy sources . (wikipedia.org)
- Enzymatic biofuel cells also have operating requirements not shared by traditional fuel cells. (wikipedia.org)
Hyaluronic1
- Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells. (sigmaaldrich.com)
Ethanol production1
- Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles. (degruyter.com)
Ligands1
- In this study, we show that receptor reorganization within the contacting mast cell membrane is markedly different upon binding of mobile and immobilized ligands. (jimmunol.org)
Peptides1
- Non-covalent immobilized artificial membrane reverse-phase high performance liquid chromatography was previously evaluated as a means whereby elution times for antimicrobial peptides from columns mimicking the lipid bilayers of different membrane systems might be used as a fast-screening method to compare relative binding effectiveness. (ualberta.ca)
Surface14
- To give new insight into the intracellular trafficking of NPs that target the CD44 receptor, which is overexpressed on the surface of a broad variety of cancer cells, we have synthesized an NP pH sensor system that targets CD44. (sigmaaldrich.com)
- The pH distribution profile in cells was measured for nanosensors with HA, cationic, and noncharged NP surface coatings giving a clear indication of the intracellular pH environment that the different NPs experience after internalization. (sigmaaldrich.com)
- Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB , gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were immobilized onto the polystyrene surface of the wells of microtiter plates using adsorption method, followed by cross-linking with glutaraldehyde. (thescipub.com)
- Scanning electron microscope and cell count analyses revealed highly dense, rod shaped bacterial cells stably attached to the well surface. (thescipub.com)
- We successfully developed a stem cell rolling column with a soft-interface bearing anti cell surface marker molecules antibody. (nii.ac.jp)
- As the results, zwitterionic telomer brush containing 2-methacryloyloxyethyl phosphorylcholine (MPC) efficiency suppressed the non-specific interaction between cells and antibody-immobilized surface. (nii.ac.jp)
- Atomic force microscopy (AFM) studies showed an even distribution of immobilized proteoliposomes on the surface. (chalmers.se)
- Further, the cell culture support is produced by pretreating the support surface with a reagent to provide hydrophobicity to the support surface. (google.com)
- The steps of applying a photoresist, partially removing it, providing hydrophobicity to the cell support surface, applying a solution to affect cell adhesiveness, film formation and dissolving the remaining photoresist are carried out repeatedly. (google.com)
- iv) dissolving the photoresist remaining on the support surface from (iii) to produce said cell culture support. (google.com)
- 2. The cell culture support of claim 1 wherein the partial removal of the photoresist by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing a irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface. (google.com)
- 4. The cell culture support of claim 1 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface. (google.com)
- 5. The cell culture support of claim 1 wherein the photoresist layer is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water. (google.com)
- 4. The method of claim 1 , wherein the device comprises a plurality of welds for maximizing surface area to volume ratio of the human PDX1-positive pancreatic progenitor cell population in the device. (google.es)
Mammalian4
- We are interested in the external and internal cues that regulate mammalian stem cell division and cell fate choice. (kcl.ac.uk)
- MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. (nature.com)
- A plausible explanation for the lack of agreement could be the different sources of BRAK used by investigators, which included a synthetic polypeptide, a murine homologue, and unpurified conditioned supernatants from transfected mammalian cells. (aacrjournals.org)
- This study reveals the practicality of liquid chromotography-mass spectrometry n neutral loss scanning for large-scale identification and quantification of protein phosphorylation in the analysis of cell signaling in a native mammalian system. (pnas.org)
Microorganism1
- The immobilized microorganism was able to actively degrade a 10% mixture of pentane, allowing us to obtain a bioconversion yield of 90% after 36h. (eugris.info)
Strain1
- Slower fer- mentation ki netics were observed in all the samples that were fermented with strain RC212 (suspende d and immobilized) than in samples fermented with strain EC1118. (vtt.fi)
Stability5
- The immobilized cells exhibited favourable operational stability. (springer.com)
- This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and screening with the advantages of simplicity, stability, ease and convenience for assembly and use, and low cost. (thescipub.com)
- The effects of different polymers of hollow fiber membrane and culture conditions on the cyclodextrin glucanotransferase (CGTase) excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. (ump.edu.my)
- The immobilized cells on a polyvinylidene fluoride polymer exhibited a 2.0-4.5-fold increase in the CGTase excretion with 18-95% reduction of cell lysis and over 100% increment of plasmid stability compared to the free cells. (ump.edu.my)
- The use of low tryptone concentration (5 g/1) reduced the occurrence of cell lysis (90% reduction) and increased the plasmid stability (86% increment) without significant change in CGTase excretion in comparison with initial tryptone concentration (20 g/1). (ump.edu.my)
Free13
- Using this cell free system, we were able to obtain about 2.6 times higher concentrations of iso-butanol using our non-optimized system as compared with live cell systems. (osti.gov)
- This integrated biocatalytic system is able to perform simultaneously AF and MLF, producing wines similar in organoleptic traits in comparison with wines fermented following traditional sequential AF and MLF with free cell starters. (frontiersin.org)
- Through phase separation, they spontaneously self-assemble into dynamic, membrane-free, dropletlike structures that can perform needed tasks in cells . (scientificamerican.com)
- The biota of all three beverages was subsequently supplemented with free and immobilized cells of B. (thefreedictionary.com)
- Figure 4 illustrates the growth curves for free and immobilized cells of Nannochloropsis sp. (thefreedictionary.com)
- The growth and metabolic profiles were evaluated for both free and immobilized cells. (comu.edu.tr)
- In this study we asked whether ImC from tumor-free and tumor-bearing hosts differ in their ability to suppress CD8 + T cells, and what could be a mechanism of Ag-specific inhibition of T cell response by ImC in cancer. (jimmunol.org)
- It appears that the Michaelis constant does not change with operating conditions and remains roughly equal to the value obtained for free cells. (inrae.fr)
- Better results were observed in effluent sample remediated with immobilized cells rather than free cells. (mahendrapublications.com)
- when assayed either as free or immobilized cells showed chlorpyrifos removal percentages of 40.17% and 71.05%, respectively, and for pentachlorophenol 5.24% and 14.72%, respectively, suggesting better removal of both pesticides by using immobilized cells. (hindawi.com)
- These results reveal that environments contaminated with mixtures of xenobiotics could be successfully cleaned up by using either free or immobilized cultures of Streptomyces , through in situ or ex situ remediation techniques. (hindawi.com)
- Experiments are carried out with free cells between 6 and 8 pH and the uptake of Fe(II) is observed to be maximum at pH 7. (bvsalud.org)
- Studies with free and immobilized cells revealed that immobilized cells are more efficient for the removal of Fe(II) than free cells . (bvsalud.org)
Wastewater1
- The anaerobically treated wastewater was treated further in immobilized Cell bioreactor of height 5 m and dia. 1 m. (mirchmasalatv.com)
Vitro2
- In addition, using an immobilized template assay we found that the ATPase component of the SWI/SNF chromatin remodeling complex, brg1, is recruited to the GM-CSF proximal promoter in an NF-κB-dependent manner in vitro. (rupress.org)
- In vitro , rBRAK blocked endothelial cell chemotaxis at concentrations as low as 1 nmol/L, suggesting this was a major mechanism for angiogenesis inhibition. (aacrjournals.org)
Cellulose1
- Synergistic enhancement of cytotoxicity against cancer cells by incorporation of rectorite into the paclitaxel immobilized cellulose acetate nanofibers. (americanelements.com)
Cultures3
- (Citrus paradisi) cell cultures. (springer.com)
- Addition of iododeoxyuridine (IdUrd) to low density cultures increased the expression of EA to 20%, whereas in confluent cultures the cells could not be induced to synthesize EA. (uni-regensburg.de)
- Immobilized Raji cells proved to be suitable targets for superinfection with EBV derived from P3HR1 cultures. (uni-regensburg.de)
Alginate4
- In cell transplantation, alginate immobilized cells are utilized to act as the barrier between the host immune system and the transplanted cell [4]. (thefreedictionary.com)
- Alginate immobilized cells have been used in cell transplantation where it acts as the barrier between transplant ( immobilized cell ) and host immune system. (thefreedictionary.com)
- Cells co-entrapped together with polyurethane into Ca-alginate did not show higher lipase production levels than the cells entrapped in Ca-alginate gels. (uab.cat)
- Encapsulation of the cells was performed by droplet formation from a foamed alginate solution. (comu.edu.tr)
Basics and Applications1
- This publication contains full papers of both oral and poster presentations of the symposium 'Immobilized Cells: Basics and Applications' that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. (elsevier.com)
Kinetic1
- The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase , as well as its kinetic parameters (K M and Vmax). (bvsalud.org)
Catalysts1
- Most organic compounds cannot be used as fuel by fuel cells with metal catalysts because the carbon monoxide formed by the interaction of the carbon molecules with oxygen during the fuel cell's functioning will quickly "poison" the precious metals that the cell relies on, rendering it useless. (wikipedia.org)
Sodium1
- It is found that the uptake has increased when Sodium chloride (NaCl) and Magnesium Chloride ( MgCl2 ) mixed cells are used. (bvsalud.org)
Efficiency1
- We came up with a big problem about the non-specific cell entrapment on the antibody-immobilized column, and it reduces the efficiency of specific cell purification based on cell-rolling. (nii.ac.jp)
Enrichment1
- The ICAT quantitative labeling strategy (3, 4) is perhaps the best-characterized method for relative protein quantitation using MS. Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine (5), lysine (6), tyrosine (7), and leucine (8), for in vivo incorporation of a mass difference to support relative quantitation. (psu.edu)
Growth8
- Immobilized-cell growth: diffusion limitation in expanding micro-colonies (R.H. Wijffels et al. ). (elsevier.com)
- In view of the advantages of the bulk production of clonal pancreaticbeta cells, an investigation was made of the growth and insulin secretoryfunctions of an electrofusion-derived cell line (BRIN-BD11) immobilizedon a solid microcarrier, cytodex-1 or a macroporous microcarrier,cultispher-G. For comparison, similar tests were performed usingBRIN-BD11 cells present in single cell suspensions or allowed toform pseudoislets. (portlandpress.com)
- Similar growth profiles were recorded for eachmicrocarrier with densities of 4.4×10 5 ±0.3 cells/ml and4.2×10 5 ±0.2 cells/ml achieved using cytodex-1 andcultispher-G, respectively. (portlandpress.com)
- Cell growth on immobilized cell-growth factor. (semanticscholar.org)
- article{Zheng1994CellGO, title={Cell growth on immobilized cell-growth factor. (semanticscholar.org)
- The method involved culturing cells with the expanded polystyrene in shake-flasks, followed by in situ growth within the column. (eugris.info)
- Tumor growth is associated with the accumulation of immature myeloid cells (ImC), which in mice are characterized by the expression of Gr-1 and CD11b markers. (jimmunol.org)
- There is now ample evidence that tumor growth in cancer patients and tumor-bearing mice is associated with an accumulation of immature myeloid cells (ImC) 3 ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ). (jimmunol.org)
Endothelial cells3
- Is the Subject Area "Endothelial cells" applicable to this article? (plos.org)
- GM-CSF is induced in a variety of cell types, including T cells, macrophages, endothelial cells, and fibroblasts in response to immune and inflammatory signals ( 1 - 3 ). (rupress.org)
- Polyurethane (PU) is widely used as a cardiovascular biomaterial due to its good mechanical properties and hemocompatibility, but it is not adhesive to endothelial cells (ECs). (aminer.cn)
Reaction1
- 1. A process for preparing phenylalanine which comprises preparing an aqueous reaction mixture consisting essentially of phenylpyruvic acid or phenylpyruvate, pyridoxal -5-phosphate as a cofactor and an amine donor selected from the group consisting of L-glutamic acid and L-aspartic acid, and contacting said reaction mixture with cells immobilized with a polyazetidine polymer, the cells having transaminase activity to obtain said phenylalanine in high yield. (google.ca)