A group of compounds that are derivatives of phenylpyruvic acid which has the general formula C6H5CH2COCOOH, and is a metabolite of phenylalanine. (From Dorland, 28th ed)
An enzyme that catalyzes the deamination of PHENYLALANINE to form trans-cinnamate and ammonia.
An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.
A species of ANABAENA that can form SPORES called akinetes.
An enzyme of the oxidoreductase class that catalyzes the formation of L-TYROSINE, dihydrobiopterin, and water from L-PHENYLALANINE, tetrahydrobiopterin, and oxygen. Deficiency of this enzyme may cause PHENYLKETONURIAS and PHENYLKETONURIA, MATERNAL. EC 1.14.16.1.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.
Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.
Volume of circulating BLOOD. It is the sum of the PLASMA VOLUME and ERYTHROCYTE VOLUME.
Books in the field of medicine intended primarily for consultation.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
An alloy of 60% cobalt, 20% chromium, 5% molybdenum, and traces of other substances. It is used in dentures, certain surgical appliances, prostheses, implants, and instruments.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The nonstriated involuntary muscle tissue of blood vessels.
Characteristics or attributes of the outer boundaries of objects, including molecules.
A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).
Flavoproteins that catalyze reversibly the reduction of carbon dioxide to formate. Many compounds can act as acceptors, but the only physiologically active acceptor is NAD. The enzymes are active in the fermentation of sugars and other compounds to carbon dioxide and are the key enzymes in obtaining energy when bacteria are grown on formate as the main carbon source. They have been purified from bovine blood. EC 1.2.1.2.
Energy transmitted from the sun in the form of electromagnetic radiation.
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
Raw and processed or manufactured milk and milk-derived products. These are usually from cows (bovine) but are also from goats, sheep, reindeer, and water buffalo.
A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
The processes of milk secretion by the maternal MAMMARY GLANDS after PARTURITION. The proliferation of the mammary glandular tissue, milk synthesis, and milk expulsion or let down are regulated by the interactions of several hormones including ESTRADIOL; PROGESTERONE; PROLACTIN; and OXYTOCIN.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.
Live microbial DIETARY SUPPLEMENTS which beneficially affect the host animal by improving its intestinal microbial balance. Antibiotics and other related compounds are not included in this definition. In humans, lactobacilli are commonly used as probiotics, either as single species or in mixed culture with other bacteria. Other genera that have been used are bifidobacteria and streptococci. (J. Nutr. 1995;125:1401-12)
A metallic element with the atomic symbol Ir, atomic number 77, and atomic weight 192.22.
A technique for analysis of the chemical composition of molecules. A substance is bombarded with monochromatic ELECTRONS. Some of the electrons passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The energy loss is element dependent. Analysis of the energy loss spectrum reveals the elemental composition of a specimen. ENERGY-FILTERED TRANSMISSION ELECTRON MICROSCOPY is a type of electron energy loss spectroscopy carried out in electron microscopes specially outfitted to analyze the spectrum of electron energy loss.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
A cell line derived from cultured tumor cells.
Unstable isotopes of iridium that decay or disintegrate emitting radiation. Ir atoms with atomic weights 182-190, 192, and 194-198 are radioactive iridium isotopes.
A pyridoxal phosphate enzyme that catalyzes the reaction of glycine and 5,10-methylene-tetrahydrofolate to form serine. It also catalyzes the reaction of glycine with acetaldehyde to form L-threonine. EC 2.1.2.1.
An enzyme of the lyase class that catalyzes the cleavage of fructose 1,6-biphosphate to form dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. The enzyme also acts on (3S,4R)-ketose 1-phosphates. The yeast and bacterial enzymes are zinc proteins. (Enzyme Nomenclature, 1992) E.C. 4.1.2.13.
A pyridoxal-phosphate protein that catalyzes the deamination of THREONINE to 2-ketobutyrate and AMMONIA. The role of this enzyme can be biosynthetic or biodegradative. In the former role it supplies 2-ketobutyrate required for ISOLEUCINE biosynthesis, while in the latter it is only involved in the breakdown of threonine to supply energy. This enzyme was formerly listed as EC 4.2.1.16.
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An essential amino acid occurring naturally in the L-form, which is the active form. It is found in eggs, milk, gelatin, and other proteins.
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Chemical substances that attract or repel cells. The concept denotes especially those factors released as a result of tissue injury, microbial invasion, or immunologic activity, that attract LEUKOCYTES; MACROPHAGES; or other cells to the site of infection or insult.

Immobilization patterns and dynamics of acetate-utilizing methanogens immobilized in sterile granular sludge in upflow anaerobic sludge blanket reactors. (1/189)

Sterile granular sludge was inoculated with either Methanosarcina mazeii S-6, Methanosaeta concilii GP-6, or both species in acetate-fed upflow anaerobic sludge blanket (UASB) reactors to investigate the immobilization patterns and dynamics of aceticlastic methanogens in granular sludge. After several months of reactor operation, the methanogens were immobilized, either separately or together. The fastest immobilization was observed in the reactor containing M. mazeii S-6. The highest effluent concentration of acetate was observed in the reactor with only M. mazeii S-6 immobilized, while the lowest effluent concentration of acetate was observed in the reactor where both types of methanogens were immobilized together. No changes were observed in the kinetic parameters (Ks and mumax) of immobilized M. concilii GP-6 or M. mazeii S-6 compared with suspended cultures, indicating that immobilization does not affect the growth kinetics of these methanogens. An enzyme-linked immunosorbent assay using polyclonal antibodies against either M. concilii GP-6 or M. mazeii S-6 showed significant variations in the two methanogenic populations in the different reactors. Polyclonal antibodies were further used to study the spatial distribution of the two methanogens. M. concilii GP-6 was immobilized only on existing support material without any specific pattern. M. mazeii S-6, however, showed a different immobilization pattern: large clumps were formed when the concentration of acetate was high, but where the acetate concentration was low this strain was immobilized on support material as single cells or small clumps. The data clearly show that the two aceticlastic methanogens immobilize differently in UASB systems, depending on the conditions found throughout the UASB reactor.  (+info)

Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (2/189)

Viable cells of Pseudomonas aeruginosa were entrapped in alginate gel layers and incubated in a minimal glucose (15 g/L)-yeast extract (2 g/L)-salt medium to form artificial biofilm-like structures. After cultivation for 2 days, the biomass distribution inside the polymer was highly heterogeneous. The cell number reached approximately 1011 cells/g gel in the outer regions of the gel structures whereas the inner areas were less colonized (c. 10(8) cells g/gel). Killing of immobilized organisms by imipenem and tobramycin were compared with free-cell experiments (inoculum c. 10(9) cells/mL). Sessile-like bacteria displayed a higher resistance to the two antibiotics used alone or in combination than did suspended cells. Exposure for 10 h to 20 x MIC imipenem and 15 x MIC tobramycin reduced the number of viable immobilized bacteria to 0.3% and 3%, respectively, of the initial cell population, whereas these antibiotic concentrations were much more efficient (bactericidal) against free-cell cultures (5 log kill in 6 h). A synergic effect of tobramycin and imipenem was detected on bacterial suspensions but not on biofilm-like structures. Effective diffusivity measurements showed that the diffusion of imipenem in the alginate layer was not hindered. A slight but significant enhancement of beta-lactamase induction in immobilized cells as compared with their suspended counterparts was insufficient to explain the high resistance of sessile-like bacteria.  (+info)

Distribution of Nitrosomonas europaea and Paracoccus denitrificans immobilized in tubular polymeric gel for nitrogen removal. (3/189)

To improve the cooperative removal of nitrogen by Nitrosomonas europaea and Paracoccus denitrificans, we controlled their distribution in a tubular gel. When ethanol was supplied inside the tubular gel as an electron donor, their distributions overlapped in the external region of the gel. By changing the electron donor from ethanol to gaseous hydrogen, the distribution of P. denitrificans shifted to the inside of the tube and was separated from that of N. europaea. The separation resulted in an increase of the oxidation rate of ammonia by 25%.  (+info)

Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. (4/189)

Bifidobacterium longum KCTC 3128 and HLC 3742 were independently immobilized (entrapped) in calcium alginate beads containing 2, 3, and 4% sodium alginate. When the bifidobacteria entrapped in calcium alginate beads were exposed to simulated gastric juices and a bile salt solution, the death rate of the cells in the beads decreased proportionally with an increase in both the alginate gel concentration and bead size. The initial cell numbers in the beads affected the numbers of survivors after exposure to these solutions; however, the death rates of the viable cells were not affected. Accordingly, a mathematical model was formulated which expressed the influences of several parameters (gel concentration, bead size, and initial cell numbers) on the survival of entrapped bifidobacteria after sequential exposure to simulated gastric juices followed by a bile salt solution. The model proposed in this paper may be useful for estimating the survival of bifidobacteria in beads and establishing optimal entrapment conditions.  (+info)

Identification of a novel glycoprotein-binding activity in Streptococcus pyogenes regulated by the mga gene. (5/189)

The interaction between Streptococcus pyogenes and the host cell surface is not completely understood. Characterization of the adhesion mechanisms of the bacterium to the host cell surface is needed in order to develop new vaccines and anti-adhesion drugs. The presence of glycoprotein-binding activities among streptococcal strains was investigated. An activity binding to thyroglobulin, fetuin, asialofetuin and mucin but not non-glycosylated proteins was found to be present in the majority of the S. pyogenes strains studied. Cross-inhibition experiments suggested that the glycoproteins share a common structure recognized by the bacteria. The glycoprotein-binding activity was found to be proteinaceous, tightly attached to the bacterial surface and it also mediated the adherence of bacteria to solid surfaces coated with glycoproteins. The activity was found by transposon mutagenesis and complementation to be regulated by the multiple-gene regulator Mga, which has been implicated as a regulator of S. pyogenes virulence factors.  (+info)

Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface. (6/189)

Adhesion of Candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. C. albicans cells also adhere to several species of oral streptococci including Streptococcus gordonii, Streptococcus oralis and Streptococcus sanguinis in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of C. albicans cells by up to 2-3-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which C. albicans cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of S. gordonii cells but not to S. oralis cells. Parotid saliva that was pre-adsorbed with S. gordonii cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of C. albicans compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. C. albicans cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of C. albicans to S. gordonii cells. The results demonstrate that C. albicans cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote C. albicans adhesion and oral colonization.  (+info)

Response kinetics of tethered Rhodobacter sphaeroides to changes in light intensity. (7/189)

Rhodobacter sphaeroides can swim toward a wide range of attractants (a process known as taxis), propelled by a single rotating flagellum. The reversals of motor direction that cause tumbles in Eschericia coli taxis are replaced by brief motor stops, and taxis is controlled by a complex sensory system with multiple homologues of the E. coli sensory proteins. We tethered photosynthetically grown cells of R. sphaeroides by their flagella and measured the response of the flagellar motor to changes in light intensity. The unstimulated bias (probability of not being stopped) was significantly larger than the bias of tethered E. coli but similar to the probability of not tumbling in swimming E. coli. Otherwise, the step and impulse responses were the same as those of tethered E. coli to chemical attractants. This indicates that the single motor and multiple sensory signaling pathways in R. sphaeroides generate the same swimming response as several motors and a single pathway in E. coli, and that the response of the single motor is directly observable in the swimming pattern. Photo-responses were larger in the presence of cyanide or the uncoupler carbonyl cyanide 4-trifluoromethoxyphenylhydrazone (FCCP), consistent with the photo-response being detected via changes in the rate of electron transport.  (+info)

Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system. (8/189)

A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain GCH1 grew on propachlor with a generation time of 4.2 h and a rate of substrate utilization of 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but was subject to catabolite expression. Acetanilide was a growth substrate with a yield of 0.56 +/- 0.02 mg of protein micromol(-1). GCH1 strain cells were immobilized by adsorption onto a ceramic support and were used as biocatalysts in an immobilized cell system. Propachlor elimination reached 98%, with a retention time of 3 h and an initial organic load of 0.5 mM propachlor. The viability of immobilized cells increased 34-fold after 120 days of bioreactor operation.  (+info)

TY - JOUR. T1 - High alcohol production by repeated batch fermentation using an immobilized osmotolerant Saccharomyces cerevisiae. AU - Sree, NK. AU - Sridhar, M. AU - Suresh, K. AU - Banat, Ibrahim. AU - Rao, LV. PY - 2000/3. Y1 - 2000/3. N2 - A repeated batch fermentation system was used to produce ethanol using an osmotolerant Saccharomyces cerevisiae (VS3) immobilized in calcium alginate beads, For comparison free cells were also used to produce ethanol by repeated batch fermentation. Fermentation was carried for six cycles with 125, 250 or 500 beads using 150, 200 or 250 g glucose L-1 at 30 degrees C, The maximum amount of ethanol produced by immobilized VS, using 150 g L-1 glucose was only 44 g L-1 after 48 h, while the amount of ethanol produced by free cells in the first cycle was 72 g L-1. However in subsequent fed batch cultures more ethanol was produced by immobilized cells compared to free cells. The amount of ethanol produced by free cells decreased from 72 g L-1 to 25 g L-1 after ...
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Whole cell immobilization of thermostable ?-amylase from phosphate solubilizing Bacillus thuringiensis A5-BRSC-IJMR-Print ISSN No:-2394-546X Online ISSN No:-2394-5478Article DOI No:-10.18231/2394-5478.2018.0055,Indian Journal of Microbiology Research-IP Innovative Publication Pvt Limited, Medical Journals Publication
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A biosensor is proposed that contains bacteria that naturally effluxes potassium ions when threatened by electrophilic species. Pseudomonas aeruginosa is an activated sludge isolate and possesses the characteristic potassium efflux response. It has been immobilized in calcium alginate beads, photopolymer disks, and a thermally reversible gel in order to ultimately incorporate the immobilized system into a functional biosensor. The potassium efflux and cell viability were measured in the immobilized matrices. Wastewater treatment is of utmost importance; however, processes are easily upset. Upsets can be caused by various electrophiles found in the environment, and can cause serious health effects to people or the environment downstream from an upset. Electrophiles can cause the activated sludge in wastewater treatment facilities to deflocculate, and untreated water can be lost downstream. Devising a detection system for proactively sensing electrophiles prior to an upset is an important ...
This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed …
Figure 1:Fluorescence images of PC3-GFP cells immobilized on ECM micro patterns of various shapes (the patterns are depicted by dashed lines).. Mastering the adhesion landscape of living cells on a surface is emerging as a new tool for investigating a lot of fundamental mechanisms of cell biology such as shape control, differentiation, division, polarity or motility. The common point of all these studies is the production of micro-patterns of various shapes and dimensions along which, adherent cells are immobilized in a deterministic way. The control of cell adhesion through technology allows the biologists to design specific scenarii and record how various living cells respond (neural, epithelial, tumoral and stem cells). Because technology allows reproducing identical precise patterns along well arranged periodical arrays, these experimental observations can be made systematically over large population of cells thus reaching a high level of representativeness. The micro-contact printing of ...
Selim, N., Hamed, H., Fathy, R., Gamal, M., El Kareem, H.A., Breheny, M., Farahmand, N., Bowman, K., Kyazze, G., Keshavarz, T. and Gomaa, O.M. 2018. Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation, salinity and endogenous biosurfactant induction. 4th European Meeting of the International Society for Microbial Electrochemistry and Technology. Newcastle University, Newcastle upon Tyne 12 - 14 Sep 2018 Design of a single chamber air cathode microbial fuel cell using a stainless steel spiral anode and 3D printing techniques for continuous flow dye decolourisation ...
Cells of a commercial Saccharomyces cerevisiae strain (Uvaferme 299) were immobilized on dried raisin berries (Sultanina variety) to produce an immobilized biocatalyst for use in dry white wine-making. The immobilised biocatalyst was found to be suitable for wine making at ambient temperatures (15-25 °C). The wines produced had low volatile acidities and low methanol and acetaldehyde contents, while volatile by-products showed no statistically significant differences from wines produced by free cells. The immobilized cell system had a good operational stability for more than 4 months. Sensory evaluation revealed differences between wines produced by immobilized and free cells ...
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Description: The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants ...
Description: A polyclonal antibody against SAMM50. Recognizes SAMM50 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1: ...
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Upon ligand binding, Fas forms the death-inducing signaling complex (DISC), a multi-protein complex formed by members of the death receptor family of apoptosis-inducing cellular receptors. The DISC is composed of the death receptor, FADD, and caspase 8.
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Fingerprint Dive into the research topics of Improved lactic acid productivity by an open repeated batch fermentation system using Enterococcus mundtii QU 25. Together they form a unique fingerprint. ...
Biocatalytic asymmetric reductions with whole cells can offer high enantioselectivity, environmentally benign processes and energy-effective operations and thus are of great interest. The application of whole cell-mediated bioreduction is often restricted if substrate and product have low water solubility and/or high toxicity to the biocatalyst. Many studies have shown that a biphasic system is often useful in this instance. Hence, we developed efficient biphasic reaction systems with biocompatible water-immiscible ionic liquids (ILs), to improve the biocatalytic anti-Prelog enantioselective reduction of acetyltrimethylsilane (ATMS) to (R)-1-trimethylsilylethanol {(R)-1-TMSE}, which is key synthon for a large number of silicon-containing drugs, using immobilized Candida parapsilosis CCTCC M203011 cells as the biocatalyst. It was found that the substrate ATMS and the product 1-TMSE exerted pronounced toxicity to immobilized Candida parapsilosis CCTCC M203011 cells. The biocompatible water-immiscible ILs
β-Fructofuranosidase, an hydrolyzing β-1,6-fructosidic linkage, was immobilized covalently on the oxidized microbial cell wall of a Penicillium species (PS-8), which is totally different from the conventional whole cell immobilization. The microbial cells of the PS-8 strain, which has a fluffy characteristic during cultivation, was treated with sodium metaperiodate to form active aldehyde groups on them. The periodate treated cells were reacted with the β-fructofuranosidase. This mixture of cell and the enzyme was again treated with 0.5% glutaraldehyde for 1 hr and finally spinned with a syringe (diameter 1 mm) to form pellets, and dried at air. By determining the effects of periodate concentration on the aldehyde formation of microbial cells, the optimal treating concentration of periodate was 1.2 grams per gram of the dry weight and the formation of the aldehyde was 35% as the % dialdehyde units. The reaction temperature did not give any effect on the formation of aldehyde of the microbial ...
Supported gold nanoparticles are used for a wide range of catalytic processes. In this work, we use dispersion corrected density functional theory (DFT-D) to study the effect of commonly used support materials (MgO, C, CeO2) on small gold particles with up to 19 atoms. Our results show that the preferred cluster shape and morphology is highly dependent on the support material due to different adsorption strength and structural mismatch between the cluster and the surface material. We developed an algorithm to measure the mismatch between the cluster interface and the support surface. Moreover, depending on the support material, the gold clusters exhibit a positive or negative polarisation, which ultimately has strong implications on the catalytic activity of such particles. This behaviour is rationalised by an analysis of the electronic structure of the metal particles and support materials.. ...
BIOLOGISCHE REAKTOREN (BIOTECHNOLOGIE); CYTOLOGISCHE BIOTECHNOLOGIE; IMMOBILISIERTE ZELLEN + IMMOBILISIERUNG VON ZELLEN (CYTOLOGISCHE METHODEN); SEKUNDÄRMETABOLITEN (PFLANZENCHEMIE); MEMBRANREAKTOREN (CHEMISCHE VERFAHRENSTECHNIK); BIOLOGICAL REACTORS (BIOTECHNOLOGY); CYTOLOGICAL BIOTECHNOLOGY; IMMOBILIZED CELLS + CELL IMMOBILIZATION (CYTOLOGICAL TECHNIQUES); SECONDARY METABOLITES (PHYTOCHEMISTRY); MEMBRANE REACTORS (CHEMICAL ENGINEERING ...
A method of recycling a support material for supporting images thereon, at least part of the support material including a paper layer which contains cellulose fibers as the main component and bears th
A support material for solid phase synthesis is provided having an amine-containing organic group attached to it through a linker. The support material is of the following general formula (Formula I): ##STR1##
Described is a photographic support material for black-and-white and color photographic emulsions, which support material is coated at least on one side with a layer containing a white pigment ad a bi
The internal structure of aerobic granules has a significant impact on the hydrodynamic performance and mass transfer process, and severely affects the efficiency and stability of granules-based reactors for wastewater treatment. In this study, for the first time the granule complex structure was correlated with the hydrodynamic performance and substrates reactions process. First, a series of multiple fluorescence stained confocal laser scanning microscopy images of aerobic granules were obtained. Then, the form and structure of the entire granule was reconstructed. A three-dimensional computational fluid dynamics study was carried out for the hydrodynamic analysis. Two different models were developed on the basis of different fluorescence stained confocal laser scanning microscopy images to elucidate the roles of the granule structure in the hydrodynamic and mass transfer processes of aerobic granules. The fluid flow behavior, such as the velocity profiles, the pathlines and hence the ...
The biotransformation of water insoluble substrates by mammalian and bacterial cells has been problematic, since these whole cell reactions are primarily performed in an aqueous environment The implementation of a twophase or encapsulated system has the advantages of providing a low water system along with the physiological environment the cells require to sustain themselves. Encapsulation of mammalian cells by formation of polyamide capsules via interfacial polymerization illustrated that the cells could not survive this type of encapsulation process. Biotransformation of the steroid spironolactone [3] by human kidney carcinoma cells was performed in a substrate-encapsulated system, yielding canrenone [4] in 70% yield. Encapsulation of nitrile-metabolizing Rhodococcus rhodochrous cells using a polyamide membrane yielded leaky capsules, but biotransformation of 2-(4- chlorophenyl)-3-methylbutyronitrile (CPIN) [6] in a free cell system yielded CPIN amide [7] in 40% yield and 94% ee. A two-phase ...
Stirred-tank bioreactors are gaining more and more importance in the industrial production of pharmaceutical products, biofuels and food. When compared with 2D-cultivation systems, bioreactors are easy to handle, and multiple bioreactors can be controlled in parallel without a considerable increase of workload. In stirred-tank bioreactors cells can be cultivated as free cells in solution, cell aggregates or on microcarriers in batch, fed-batch or continuous cultivation modes ...
E-cadherin interacts with other E-cadherin molecules on neighboring cells to form cell-cell adhesions. E-cadherin that is not involved in these trans interactions is removed from the cell surface and replaced with newly synthesized molecules to maintain dynamic adhesions. Now, Izumi et al. (page 237) show that E-cadherins that are involved in trans interactions are excused from this endocytosis by small GTPases. Disruption of this system may free cells for migration.. By reconstituting endocytosis in membrane bilayers, the group shows that clathrin-dependent endocytosis removes E-cadherin that is not interacting in trans with other E-cadherins. E-cadherins engaged in trans interactions, however, activated Rac and Cdc42, which blocked their internalization. So far it is unclear how the trans interactions activate the G proteins.. The endocytic block is enhanced by IQGAP1, an effector of Rac and Cdc42. IQGAP1 cross-links actin filaments into bundles, and the group shows that F-actin is needed to ...
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By Greg Mills. As I write this Sunday morning early, all the Apple news sites and a lot of regular news sites are running leaked information about the iPhone launch, scheduled for Tuesday. The normally tight new product security has been breeched by even Apple in a couple of ways. What seems clear is that Apple is moving to capture greater market share with more cellular carriers to be offering iPhones and with both a lower end and higher end offering. The iPhone 4S and iPhone 5 appear to be the two new iPhone models to be announced Tuesday.. There are two main markets the cellular carriers all support. They call the two plans post-paid and pre-paid. Generally, the post-paid segment is the cream of the crop and the real money for the networks. This is the plan with a contract, generally for two years, where you get a red hot deal on a phone, or a free cell phone in exchange for signing up. With iPhones, you just pay a portion of the cost of the.... , Read more » ...
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So, not only are taxpayers funding free cell phones, were also paying for street teams to hawk them at hospitals, soup kitchens, carnivals and shelters. Apparently a cell phone is now a basic essential, like food, water and shelter, that is so necessary for a persons survival it must be provided for those who cant afford it. Whats next? An Obama car? An Obama computer? An Obama iPad? An Obama Espresso machine? I better stop. I dont want to give them any ideas ...
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Things to See and Do Downtown and the Waterfront A Contemporary Theatre (700 Union St.) Located just around the corner from the Convention Center, this regional theatre has showcased avant garde productions since 1965. Seattle Art Museum (1300 First Ave.) Even if you dont check out the exhibits in the recently expanded museum, you might want to consider checking out the fantastic cafe and gift shop. Seattle Public Librarys Central Library (1000 Fourth Ave.) Loved by many, scoffed at by some - if you havent seen it, you really should. Look for the Free Cell Phone Tour signs inside the building for a self-guided tour. Pike Place Market (First Ave. and Pike St.) More than just flying fish and crowds at Starbucks, the market is a vibrant hive of activity. Check out the many local merchants, including the anarchist bookstore Left Bank Books and the Anglophile Crumpet Shop. Top your shopping off with a dozen mini donuts made right in front of you at the Daily Dozen Doughnut Company. Seattle Great ...
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This invention relates to a process and a laminator for the continuous production of laminates of foam with facings. An upper facing and a lower facing and a web of meshwork are introduced into the foaming space of a laminator and a fluid reaction mixture is spread over the lower facing before it runs into the foaming space. The reaction mixture penetrates the web of meshwork as it foams up on its journey through the foaming space and becomes bonded to the facings.
TY - JOUR. T1 - Promotional Effect of Water in the Clean Continuous Production of Carvacrol from Carvone. AU - Benavente Donayre, Pedro. AU - Cárdenas-lizana, Fernando. AU - Keane, Mark A.. PY - 2017/10/2. Y1 - 2017/10/2. U2 - 10.1016/j.cattod.2017.09.059. DO - 10.1016/j.cattod.2017.09.059. M3 - Article. SP - 1. EP - 22. JO - Catalysis Today. JF - Catalysis Today. SN - 0920-5861. ER - ...
article{960e9b68-d84e-438d-87fb-ee35adc2a226, abstract = {The complexes formed by the reaction of [Ti(OiPr)(4)] and bicyclo-octanediols (BODOLs) 1 and 2 (1:1) are useful as chiral catalysts in asymmetric reductions and were investigated by H-1 NMR spectroscopy and computational methods. A consistent picture emerged of head-to-tail dimers being kept together via a Ti-O-Ti-O mu-oxo bridge similar to the Ti-tartrates but different from the corresponding Ti-BINOLates and Ti-TADDOLates.}, author = {Sarvary, Ian and Norrby, P-O and Frejd, Torbjörn}, issn = {1521-3765}, language = {eng}, number = {1}, pages = {182--189}, publisher = {John Wiley & Sons}, series = {Chemistry: A European Journal}, title = {Structure investigation of Ti-IV-BODOLates involved in the catalytic asymmetric reduction of ketones using catecholborane}, url = {http://dx.doi.org/10.1002/chem.200305202}, volume = {10}, year = {2004 ...
A gentle exfoliating natural loofa scrub with the soap built right inside. These pure vegetable oil soaps are moisturizing and cleansing. You can choose from Almond Cocoa Butter which is extremely lubricating and softening, while the Cucumber is soothing and relaxing. The loofa softens when wet, sloughing off dry rough layers of skin instantly. This loofa will last for months in your shower.The loofa is about 6
A new nad-dependent alcohol-dehydrogenase with opposite facial selectivity useful for asymmetric reduction and cofactor regeneration ...
Primo Vision allows you to follow the whole sequence of embryo development in an undisturbed and ideal environment. With increased information on embryo development based on detailed analysis you can confidently select the best embryos for transfer
IBM Storage Networking SAN512B-6 and SAN256B-6 directors combine hardware, software and instrumentation for operational stability and application performance
Iron Fit Nutrition strives to produce distinctive, affordable, premium-quality products while making every effort to anticipate ever-changing customer needs and meet the demands of a dynamic market with innovative products, effective support materials, and unsurpassed service ...
This video from Ethics Unwrapped introduces the behavioral ethics bias known as the self-serving bias. The self-serving bias causes us to see things in ways that support our best interests and our pre-existing points of view. Support materials include discussion quesions, case study, and teaching tips. This video/case study is provided by Ethics Unwrapped and is a free educational resource from The University of Texas at Austin.
Microinnova Engineering GmbH is a pioneer in the field of continuous manufacturing. Our Process Development and Engineering departments in a combined effort provide process development, engineering services, scale-up and delivery of skid-based turnkey plants for continuous production ranging from kilo-lab to pilot- and industrial quantities. We are known as batch-to-conti experts applying the appropriate technology for each unique process challenge ...
In the early 1860s three Swedes, Nils Wilhelm Mitander,Julius Ramsay and Gustaf Wittenström, were engaged by theBritish to build and run charcoal-based ironworks in India.These works, the Burwai Iron Works of the British Government inthe case of Mitander and the privately owned Kumaon Iron Worksin the case of Ramsay and Wittenström, were both to bebased on the most modern European technology. The projects werepioneering in Indian ironmaking. The ambitions were high andstakes big, but after only a few years the projects were closedand the Swedes returned home.Landscapes of Technology Transferpresents a detailedstudy of the Kumaon and Burwai Iron Works, from their firstconception to their final closure. The investigation isbasically empirical and a fundamental question is: Why were theworks never brought into full and continuous production?. The ironworks projects should be considered as processes oftechnology transfer rather than fully fledged and completedtransfers. In spite of this lack of ...
Continuous production of fatty molecules (lipids) is crucial to the maintenance of the myelin sheath that wraps nerve fibers to protect them, ensuring
... when the patient has a primary injury such as an immobilized broken bone (set in a cast or immobilized in traction), for ... "satellite cells" which help to regenerate skeletal muscle fibers, and a decrease in sensitivity to or the availability of ... Disuse of the muscles, such as when muscle tissue is immobilized for even a few days of unuse - ... accompanied by a smaller number and size of the muscle cells as well as lower protein content. In humans, prolonged periods of ...
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... cell-cell adhesion. • positive regulation of nucleic acid-templated transcription. • heart development. • actin cytoskeleton ... cadherin binding involved in cell-cell adhesion. • actin binding. • muscle alpha-actinin binding. ... Pitx2 pathway mediating cell-type-specific proliferation during development.". Cell. 111 (5): 673-85. PMID 12464179. doi: ...
The carbon is either mixed with the solution then filtered off or immobilized in a filter. ... to higher temperatures and either combusted to do work or in the case of hydrogen gas extracted for use in a hydrogen fuel cell ...
"Molecular Cell. 68 (1): 130-143.e5. doi:10.1016/j.molcel.2017.08.016. PMC 5632562. PMID 28918903.. ... The end result is to immobilize the TATA-binding protein (TBP) on DNA in order to down-regulate transcription initiation. ... In specific cell types or on specific promoters TBP can be replaced by one of several TBP-related factors (TRF1 in Drosophila, ... Compounds that trap the protein-DNA intermediate could result in it being toxic to the cell once they encounter a DNA ...
Syngas may be burned directly in internal combustion engines, turbines or high-temperature fuel cells.[27] The wood gas ... "Bioconversion of corn straw to ethanol by cellulolytic yeasts immobilized in Mucuna urens matrix". Journal of King Saud ... Electricity from wood through the combination of gasification and solid oxide fuel cells, Ph.D. Thesis by Florian Nagel, Swiss ...
The Scoville rating of these sprays are 500,000 (sabre MK9 HVS unit) and 2,000,000 (Sabre, cell buster fog delivery). This was ... The definition under regulation states "any device designed to be used for the purpose of injuring, immobilizing or otherwise ... immobilizing or otherwise incapacitating any person" is a prohibited weapon.[47] ...
The phagocytosis theory is based on the notion that phagocytes are cells that have the power to englobe foreign bodies - and ... was immobilized by the antiquated mechanical equipment. ... human vaccine obtained by genetic engineering from animal cells ...
In this condition, the glans is swollen and painful, and the foreskin is immobilized by the swelling in a partially retracted ... Skin that is under tension expands by growing additional cells.. Surgical[edit]. ...
... distributes readily in high concentrations into immune cells, has antimicrobial and natural killer cell activities, ... and is partially immobilized. In advanced scurvy there are open, suppurating wounds and loss of teeth and, eventually, death. ... Cell. 132 (6): 1039-48. doi:10.1016/j.cell.2008.01.042. PMID 18358815. Lay summary - Science Daily (March 21, 2008).. ... Plant and Cell Physiology. 45 (9): 1271-1279. doi:10.1093/pcp/pch152. PMID 15509850.. ...
An arm board is recommended for immobilizing the extremity for cannulation of the hand, the foot or the antecubital fossa in ... People with sickle-cell disease may require frequent blood transfusions. Early blood transfusions consisted of whole blood, but ...
... is popular in the automotive industry as a replacement for the lead-acid wet cell. The VRLA battery uses an immobilized ... Other primary wet cells are the Leclanche cell, Grove cell, Bunsen cell, Chromic acid cell, Clark cell, and Weston cell. The ... From top to bottom: a large 4.5-volt (3R12) battery, a D Cell, a C cell, an AA cell, an AAA cell, an AAAA cell, an A23 battery ... fuel cells, flow cells and voltaic piles.[30] Wet cell. A wet cell battery has a liquid electrolyte. Other names are flooded ...
a b c „Exposure of {alpha}2,6-sialylated lactosaminic chains marks apoptotic and necrotic death in different cell types"; ... Immobilized Lotus tetragonolobus agglutinin binds oligosaccharides containing the Lex determinant „;Liying Yan, Patricia P ... Tateno H, Uchiyama N, Kuno A, Togayachi A, Sato T, Narimatsu H, Hirabayashi J."A novel strategy for mammalian cell surface ... "O-acetyl sialic acid binding lectin as a probe for detection of subtle change on cell surface induced during acute ...
In 1975, they applied drug-conjugated antibodies for the targeted delivery of cytotoxic compounds to cancer cells.[10] They ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... or immunotoxins is a biorecognition-based approach to selectively deliver a cytotoxic drug or toxin to a specific target cell. ... as exemplified by the removal of heme peptides from blood using immobilized human serum albumin, thus laying the grounds for ...
In the case of mesenchymal stem cells, these cell types include osteoblasts (bone cells), adipocytes (fat cells), and ... Therefore, a brace is often used to immobilize the knee for one to two weeks. Evidence suggests that the hamstring tendon graft ... Stem cell treatment[edit]. Autologous stem-cell transplantation using mesenchymal stem cells (MSCs) has been used to improve ... Ligament cells differ in size, respond to different cues in the cell environment, and express different cell surface markers, ...
2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell. 125 (4): 801-14. PMID 16713569. doi:10.1016/j.cell.2006.03.032. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم ... Cell. 18 (4): 1385-96. PMC 1838996. . PMID 17392519. doi:10.1091/mbc.E06-12-1120. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط , ... doi:10.1016/j.cell.2006.09.026. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم تجاهله (مساعدة); الوسيط ,العنوان=. تم ...
50,000 per SMRT cell, or 500-1000 megabases[69][70]. 30 minutes to 4 hours[71]. $0.13-$0.60. Fast. Detects 4mC, 5mC, 6mA.[72]. ... A non-radioactive method for transferring the DNA molecules of sequencing reaction mixtures onto an immobilizing matrix during ... ruled that individuals have no property rights to discarded cells or any profits made using these cells (for instance, as a ... In this method, DNA molecules and primers are first attached on a slide or flow cell and amplified with polymerase so that ...
When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Gerald was seen in a prison cell.. Roderich (ロー ... As a small Titan begins eating her mother alive, Kaya is immobilized by a state of shock. She is rescued when Sasha (who is ... When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Roderich was seen in a prison cell.. Civilians[ ... When Historia is sworn in as the Queen of the Walls following Rod Reiss' death, Aurille was seen in a prison cell.. Deltoff ( ...
This technique has been used to control ligand and cell binding activity, based on a variety of triggers including temperature ... The dressing design presents proprietary super-absorbent synthetic smart polymers immobilized in the 3-dimensional fiber matrix ... reactions using smart polymers is to prepare recombinant proteins with built-in polymer binding sites close to ligand or cell ...
... where membranes are cut into squares and immobilized. A more recent method involved the use of live cells that are attached to ... It works because ions from the mobile phase interact with the immobilized ions on the stationary phase, thus "shielding" the ... Ionizable molecules that are to be purified compete with these exchangeable counterions for binding to the immobilized charges ... Among the most common immobilized charged groups used are trimethylaminoethyl (TAM), triethylaminoethyl (TEAE), diethyl-2- ...
"Cell. 135 (3): 535-48. doi:10.1016/j.cell.2008.09.057. PMC 2585749 . PMID 18984164.. ... stargazin is essential for immobilizing AMPA receptors in the PSD by interacting with PSD-95.[44] PSD-95 stabilizes AMPA ... Shepherd JD, Huganir RL (2007). "The cell biology of synaptic plasticity: AMPA receptor trafficking". Annu. Rev. Cell Dev. Biol ... doi:10.1016/j.cell.2006.08.037. PMID 17018279.. *^ Kim DY, Kim SH, Choi HB, Min C, Gwag BJ (2001). "High abundance of GluR1 ...
"Cell. 167 (3): 633-642.e11. doi:10.1016/j.cell.2016.09.028. PMC 5484524. PMID 27768887.. ... Cobras, vipers, and closely related species use venom to immobilize, injure or kill their prey. The venom is modified saliva, ... The thymus is located in fatty tissue above the heart and is responsible for the generation of immune cells in the blood. The ...
Caught prey gets trapped between the spikes on femur and tibia and is now immobilized. The mantis then starts eating the still ... The eyes of M. religiosa are apposition eyes with eight types of photoreceptor cells and therefore best adapted for daylight ... Yager, David; Hoy, Ronald (December 1987). "The midline metathoracic ear of the praying mantis, Mantis religiosa". Cell and ...
An array containing immobilized allele-specific oligonucleotide (ASO) probes.. *Fragmented nucleic acid sequences of target, ... DNA copy number profile for the T47D breast cancer cell line (Affymetrix SNP Array) ... LOH profile for the T47D breast cancer cell line (Affymetrix SNP Array) ... "Integrative genomic deconvolution of rheumatoid arthritis GWAS loci into gene and cell type associations". Genome Biology. 17 ( ...
There is a three-dimensional network of cell processes associated with collagen in the tendon. The cells communicate with each ... In rabbits, collagen fascicles that are immobilized have shown decreased tensile strength, and immobilization also results in ... Mechanical forces can be transmitted by focal adhesion sites, integrins, and cell-cell junctions. Changes in the actin ... "Tendon cells in vivo form a three dimensional network of cell processes linked by gap junctions". Journal of Anatomy. 189 (Pt 3 ...
"Adoptive immunotherapy of lung cancer with immobilized anti-TCRgammadelta antibody-expanded human gammadelta T-cells in ... Immune effector cells such as lymphocytes, macrophages, dendritic cells, natural killer cells (NK Cell), cytotoxic T ... CD4+ helper T cells, cytotoxic CD8+ T cells and B cells). This initiates a cytotoxic response against tumor cells expressing ... T-cell adoptive transfer[edit]. Adoptive cell transfer in vitro cultivates autologous, extracted T cells for later transfusion. ...
Red blood cells and fibrin are the main components of venous thrombi,[6] and the thrombi appear to attach to the blood vessel ... In adults who have had their lower leg casted, braced, or otherwise immobilized for more than a week, LMWH may decrease the ... and this state is linked to the activation of white blood cells (leukocytes) and the endothelium. Specifically, the two ...
This behavior also extends into adulthood, when a male will grab the female by the scruff to immobilize her while he mounts, ... This depolarization sensitizes muscle cells so they are more likely to contract. The stomach is not only a muscular structure, ...
... a model for a quantitative study of cell adhesion". J Cell Sci 56: 21-48. PMID 7166565.. ... GE Healthcare Life Sciences, Immobilized lectin. *↑ Min W, Dunn AJ, Jones DH (1992). "Non-glycosylated recombinant pro- ... "J Cell Biol 65 (1): 123-146. PMC 2111157. PMID 1092699. doi:10.1083/jcb.65.1.123.. ... "J Cell Biol 59 (1): 134-142. PMC 2110924. PMID 4201706. doi:10.1083/jcb.59.1.134.. ...
Gordon MT, Anderson DC, Sharpe PT (1991). "Canine distemper virus localised in bone cells of patients with Paget disease". Bone ... occurs when a person with several bones affected by Paget's disease and a high serum alkaline phosphatase level is immobilized. ... The osteolysis is followed by a compensatory increase in bone formation induced by the bone forming cells, called osteoblasts, ... bone cells).[5] However, about 10-15 percent of people that develop the disease without any family history also have a mutation ...
Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell. Lactoferrin binds to the ... such as agarose with the immobilized single-stranded DNA. Lactoferrin's primary role is to sequester free iron, and in doing so ... Such an indirect antiviral effect is achieved by affecting natural killer cells, granulocytes and macrophages - cells, which ... Cell Biol. 80 (1): 125-30. doi:10.1139/o01-208. PMID 11908636. Harmsen MC, Swart PJ, de Béthune MP, Pauwels R, De Clercq E, The ...
... while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells ... while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells ... while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells ... while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells ...
Whole cell immobilization of thermostable ?-amylase from phosphate solubilizing Bacillus thuringiensis A5-BRSC-IJMR-Print ISSN ... amylase produced form the immobilized cells found to be more potent than the enzyme produced by the free cells.. Keywords:Cell ... Whole cell immobilization of thermostable ?-amylase from phosphate solubilizing Bacillus thuringiensis A5-BRSC. Full Text PDF ... Results: Immobilization of Bacillus thuringiensis A5-BRSC cells in calcium alginate resulted in more than 3.5 times higher ?- ...
The immobilized cell system had a good operational stability for more than 4 months. Sensory evaluation revealed differences ... Cells of a commercial Saccharomyces cerevisiae strain (Uvaferme 299) were immobilized on dried raisin berries (Sultanina ... while volatile by-products showed no statistically significant differences from wines produced by free cells. ... variety) to produce an immobilized biocatalyst for use in dry white wine-making. The immobilised biocatalyst was found to be ...
Cells immobilized in alginate beads seemed to efflux four times less potassium than did planktonic controls, while cells in ... This result may indicate a tighter matrix around the alginate immobilized cells, not allowing proper diffusion of potassium out ... The potassium efflux and cell viability were measured in the immobilized matrices. Wastewater treatment is of utmost importance ... It has been immobilized in calcium alginate beads, photopolymer disks, and a thermally reversible gel in order to ultimately ...
... adherent cells are immobilized in a deterministic way. The control of cell adhesion through technology allows the biologists to ... Figure 1:Fluorescence images of PC3-GFP cells immobilized on ECM micro patterns of various shapes (the patterns are depicted by ... Cell immobilization on patterned substrate. Cell immobilization on patterned substrate keywords: cellular growth, microcontact ... As seen in figure 1, adherent cells can be immobilized on-demand on the printed patterns. ...
Immobilized cell technology is a method of air filtration and purification that uses whole cell immobilization. It is a process ... Oxidation through immobilized cell technology is 12 times more efficient than natural oxidation is under the same conditions. ... As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that ... The key difference between traditional air filtration systems and those that employ immobilized cell technology is that the ...
... in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required ... The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is ... However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. Zaushitsyna, O.; ... Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience.This is only ...
The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free ... The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also ... for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having ... as well as other forms of immobilized cells, provided the immobilized cells have transaminase activity. According to the ...
Purchase Immobilized Cells: Basics and Applications, Volume 11 - 1st Edition. Print Book & E-Book. ISBN 9780444819840, ... Immobilized-cell reactors. Scale up of immobilized-cell reactors (J.J. Heijnen). PEGASUS: innovative biological nitrogen ... Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). Immobilized cell technology in food processing (C. ... Immobilized-cell growth: diffusion limitation in expanding micro-colonies (R.H. Wijffels et al.). Competition and cooperation ...
... but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. Herein ... Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, ... Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the ... Previous Document: Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon .... ...
... as well as cell elongation, while collagen immobilized surfaces greatly affect cell differentiation. ... are upregulated on collagen immobilized surfaces. These results suggest that nanotopography affects cell adhesion, ... The nanowire surfaces were fabricated from polycaprolactone and were immobilized with collagen. The objective of this study is ... The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that ...
... an alternate simplified biofuel production scheme was tested based on a cell-free immobilized enzyme system. Using this cell ... 09 BIOMASS FUELS; biofuel; cell-free; enzymes; immobilized; isobutanol; pervaporation; brush membranes.. ... Title: Optimizing Immobilized Enzyme Performance in Cell-Free Environments to Produce Liquid Fuels ... Technical Report: Optimizing Immobilized Enzyme Performance in Cell-Free Environments to Produce Liquid Fuels ...
The use of immobilized cells o. N. tabacum in sodium alginate gel much improved the yield of the products; the glycosylation of ... Glycosylation β-thujaplicin β-glucoside β-gentiobiosid Nicotiana tabacum immobilized cells This is a preview of subscription ... High Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum. ... were obtained through the biotransformation with immobilized cells in the medium without iron ions. In comparison with the case ...
The fermentation capacity of the immobilized yeast cells was found to be practically independent of the hydrogen ion ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. The fermentation capacity of the ... The fermentation capacity was maximal at 30 degrees C. The immobilized yeast cells were used for continuous production of ...
Probiotic sour milk production using cells immobilized on wheat bran. 5th World Congress on Biotechnology. A Terpou, M ... Compared to a sour milk product produced with free cells, the batch sour milk production is more costly effective since the ... The results of the present study demonstrated the successful use of freeze dried lactobacilli immobilized on wheat bran for ... Biology JournalBiomaterial journalBiotechnology JournalCell Science JournalChromosome Abnormalities JournalEvolutionary Biology ...
Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes J. Cao, C. ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes ... Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ...
Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells.. [ ... Cell uptake studies showed significant uptake of HA-coated nanosensors in HeLa cells and no uptake under the same conditions ... It is not clear if NP size, surface chemistry, and/or targeting of cell surface receptors changes the intracellular fate of NPs ... The pH distribution profile in cells was measured for nanosensors with HA, cationic, and noncharged NP surface coatings giving ...
... and 0.1 g/ml of immobilized SHMT cells at pH 7.5 and 45°C. Under these conditions, the immobilized cells were continuously used ... Preparation of optically active β-hydroxy-α-amino acid by immobilized Escherichia coli cells with serine hydroxymethyl ... The immobilized cells exhibited favourable operational stability.. Keywords. d-threo-Amino acid Enzymatic resolution ... serine were used in the resolution process catalyzed by immobilized Escherichia coli cells with SHMT activity. It was observed ...
Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-( ... the cells were immobilized in sodium alginate beads since immobilized cells had several advantages over suspended cells [16]. ... Such immobilized and stored cells can regain their viability upto several months (1-3 months) which can be an important ... The method of using L. sphaericus D3 cells immobilized in natural gel alginate beads was stable for longer periods, and with ...
Immobilized chemoattractant peptides mediate adhesion and distinct calcium-dependent cell signaling in human neutrophils. ... The reference peptide generated no such activation, and the cells maintained a more spherical morphology, suggesting that we ... Chemotaxis is the stimulated directional migration of cells in response to chemotactic factors, manifested for instance during ... To accomplish defined circumstances for recruitment and activation of cells, we fabricated semitransparent gold-coated glass ...
The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized-cell system, that we here describe for the first time in our knowledge, offers many advantages over ... In recent years, the use of immobilized cell systems has been investigated, with interesting results, for the production of ...
Zawadzki, K., Broach, J. A Rapid Technique for the Visualization of Live Immobilized Yeast Cells. J. Vis. Exp. (1), e84, doi: ... Cell 2006.. Protocol. * Immobiliserade celler för tidsförloppet epifluorescence mikroskopi. Observera att detta protokoll är ... Min mikroskopi visade att det inte finns något samband mellan den epigenetiska tillstånd HML och HMR i en enskild cell. sir1 ... Detta är en snabb teknik som immobilizes jästceller samtidigt tillåta tillväxt. Vi har följt jäst tillväxt för upp till tio ...
Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ...
... hydrogel platform to evaluate the effects of substrate stiffness and cell adhesive ligands on VEC phenotype and expression of ... and 20 kDa were polymerized into platforms of different rigidities and thiol-modified cell adhesive peptides were covalently ... have unique phenotypic responses relative to other types of vascular endothelial cells and have highly sensitive hemostatic ... Endothelial cells Is the Subject Area "Endothelial cells" applicable to this article? Yes. No. ...
Cell Sciences ®. 65 Parker Street, Unit 11. Newburyport MA 01950. Toll Free 888-769-1246 ...
Evidence for direct contacts between mast cells and other immune cells (21-23) indicates that mast cell synapses may play a ... Given recent evidence for mast cells as bona fide immune modulatory cells (21-23), synapses between mast cells and other ... 3). TIRF imaging of GFP-actin cells indicated that actin became more concentrated at the cell periphery (ring-like) after cells ... and linker for activation of T cells, were also analyzed. Stimulation of mast cells with immobilized polyvalent ligand resulted ...
BRIN-BD11 cells immobilized on cytodex-1 or cultispher-Gout-performed single cell suspensions and pseudoislets in terms ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ... Similar growth profiles were recorded for eachmicrocarrier with densities of 4.4×105±0.3 cells/ml and4.2×105±0.2 cells/ml ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ...
... mucosal addressin cell adhesion molecule-1)of epithelial cells; NT=Not tested. ... Inhibition of integrin alpha4-beta7 binding to immobilized MAdCAM-1 ( ...
All experiments were carried out using dry immobilized yeast cells instead of wet immobilized yeast cells as in previous works ... the high malate fermentation activity of the immobilized yeast cells was similar to that of the non-immobilized cells. ... Alginate bead quality tests showed that immobilized cells could be recycled up to five times without liberation of yeast cells ... Malic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe Sofía Silva, Felipe Ramón-Portugal, Patricia ...
... cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell ... In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment ... Our results show that bioactive moieties, particularly fibronectin fragment III8-10, improve cell adhesion on titanium and ... Implant materials require optimal biointegration, including strong and stable cell-material interactions from the early stages ...
Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ... Utilization of a waste glycerol fraction using immobilized cell extract from Gluconobacter oxydans ATCC 621 and its possible re ...
... carrier-bound or immobilized cell; preparation thereof > Enzyme or microbial cell is immobilized on or in an organic carrier > ... Method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell ... Name: Chemistry: molecular biology and microbiology > Carrier-bound or immobilized enzyme or microbial cell; ... Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode. Jun. 9, 2009. ...
This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and ... The immobilized cells were used as a bio-component for spectrophotometric detection of OP compounds, with MP as a ... Scanning electron microscope and cell count analyses revealed highly dense, rod shaped bacterial cells stably attached to the ... Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading ...
Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells ... Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells. ru_RU. ... BINASE IMMOBILIZED ON HALLOYSITE NANOTUBES EXERTS ENHANCED CYTOTOXICITY TOWARD HUMAN COLON ADENOCARCINOMA CELLS ... Binase Immobilized on Halloysite Nanotubes Exerts Enhanced Cytotoxicity toward Human Colon Adenocarcinoma Cells//Frontiers in ...
... oeni cells immobilized on DCM took place at 27 degrees C. From the 3rd MLF batch up to 10th, the malic acid degradation was ... and the cfu of the immobilized cells/g of biocatalyst remained stable. The produced lactic acid was less than the ... batches of white must of 11-12 degrees Be initial density were performed by Saccharomyces cerevisiae cells immobilized on ... Oenococcus oeni ATCC 23279 cells immobilized on delignified cellulosic material (DCM) were used for malolactic fermentation ( ...
  • A repeated batch fermentation system was used to produce ethanol using an osmotolerant Saccharomyces cerevisiae (VS3) immobilized in calcium alginate beads, For comparison free cells were also used to produce ethanol by repeated batch fermentation. (ulster.ac.uk)
  • It has been immobilized in calcium alginate beads, photopolymer disks, and a thermally reversible gel in order to ultimately incorporate the immobilized system into a functional biosensor. (vt.edu)
  • The micro-contact printing of proteins of the Extra Cellular Matrix (ECM) on conventional glass slides or coverslips turned out to be an efficient method for fabricating these micro-patterned cell culture substrates. (biosoftlab.com)
  • Fermentation was carried for six cycles with 125, 250 or 500 beads using 150, 200 or 250 g glucose L-1 at 30 degrees C, The maximum amount of ethanol produced by immobilized VS, using 150 g L-1 glucose was only 44 g L-1 after 48 h, while the amount of ethanol produced by free cells in the first cycle was 72 g L-1. (ulster.ac.uk)
  • amylase activity than free cells within 48 hours of batch fermentation. (innovativepublication.com)
  • The technique has several advantages including easy separation of the fermented product, least chance of contamination and no chance of wash out of cells during product recovery. (innovativepublication.com)
  • amylase produced from Calcium alginate entrapped cells of Bacillus thuringiensis A5-BRSC was purified by ammonium sulphate precipitation, followed by DE-52 ion exchange column chromatography. (innovativepublication.com)
  • Immobilization of the whole cell is a modern approach to fermentative production of different enzymes. (innovativepublication.com)
  • The amount of ethanol produced by free cells decreased from 72 g L-1 to 25 g L-1 after the fourth cycle, while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells using 150, 200 and 250 g glucose L-1 was 72.5, 93 and 87 g ethanol L-1 at 30 degrees C. (ulster.ac.uk)
  • However in subsequent fed batch cultures more ethanol was produced by immobilized cells compared to free cells. (ulster.ac.uk)
  • amylase produced form the immobilized cells found to be more potent than the enzyme produced by the free cells. (innovativepublication.com)
  • The control of cell adhesion through technology allows the biologists to design specific scenarii and record how various living cells respond (neural, epithelial, tumoral and stem cells). (biosoftlab.com)
  • Cells immobilized in alginate beads seemed to efflux four times less potassium than did planktonic controls, while cells in thermally reversible gels effluxed a comparable amount of potassium as planktonic controls. (vt.edu)
  • Mastering the adhesion landscape of living cells on a surface is emerging as a new tool for investigating a lot of fundamental mechanisms of cell biology such as shape control, differentiation, division, polarity or motility. (biosoftlab.com)
  • Biosoft has developed an instrument (Innostamp40) and a dedicated protocol enabling the automated production of highly reproducible functional micro-patterns in order to bring this process to a level where it could become a standard in cell biology. (biosoftlab.com)
  • Figure 1:Fluorescence images of PC3-GFP cells immobilized on ECM micro patterns of various shapes (the patterns are depicted by dashed lines). (biosoftlab.com)
  • Because technology allows reproducing identical precise patterns along well arranged periodical arrays, these experimental observations can be made systematically over large population of cells thus reaching a high level of representativeness. (biosoftlab.com)
  • However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. (wikipedia.org)
  • To increase stability and conversion efficiency with easy separations, the first two enzymes were immobilized onto methacrylate resin. (osti.gov)
  • Immobilized enzymes from Geotrichum spp. (semanticscholar.org)
  • Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. (bioportfolio.com)
  • Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields. (bioportfolio.com)
  • An enzymatic biofuel cell is a specific type of fuel cell that uses enzymes as a catalyst to oxidize its fuel, rather than precious metals. (wikipedia.org)
  • This offers a couple of advantages for enzymatic biofuel cells: Enzymes are relatively easy to mass-produce and so benefit from economies of scale , whereas precious metals must be mined and so have an inelastic supply . (wikipedia.org)
  • if not immobilized, the enzymes will diffuse into the cell's fuel and most of the liberated electrons will not reach the electrodes, compromising its effectiveness. (wikipedia.org)
  • The former technique is possible only with certain types of enzymes whose activation sites are close to the enzyme's surface, but doing so presents fewer toxicity risks for fuel cells intended to be used inside the human body. (wikipedia.org)
  • [1] Research on using enzymes directly for oxidation in biofuel cells began in the early 1960s, with the first enzymatic biofuel cell being produced in 1964. (wikipedia.org)
  • While the early cells did successfully produce electricity , there was difficulty in transporting the electrons liberated from the glucose fuel to the fuel cell's electrode and further difficulties in keeping the system stable enough to produce electricity at all due to the enzymes' tendency to move away from where they needed to be in order for the fuel cell to function. (wikipedia.org)
  • Increased quantities of synthesized enzymes were proportional to the increment of initial biomass present in beads, ant to a lesser degree, depended on quantities of immobilized inoculum. (olsztyn.pl)
  • In repeated batch culture, the bacteria immobilized in alginate were able to synthesize 32% more enzymes than those immobilized in chitosane. (olsztyn.pl)
  • Enzymes or whole cells physically confined or localised in a certain defined region of space with retention of their catalytic activities and which can be used repeatedly and continuously. (slideserve.com)
  • A general development of the engineering aspects of the methods will precede the description of specific applications of immobilized enzymes and microbial cells. (springer.com)
  • Zaborsky, O. R.: Immobilized Enzymes. (springer.com)
  • Immobilized Enzymes for Industrial Reactors. (springer.com)
  • During pH stability studies of soluble and immobilized enzymes the activity buffer was replaced with the same buffer used during the stability studies (50 mM sodium acetate buffer (C[H. (thefreedictionary.com)
  • The physical structure and chemical composition of support can influence the micro-environment of the immobilized enzymes, and consequently their biological properties [2, 3]. (thefreedictionary.com)
  • This invention relates to the method for immobilization or fixation of enzymes or enzyme-containing microoganism cells by which enzymes are made insoluble in water and fixed on a vehicle or carrier. (google.com)
  • So, if a method to insolubilize enzymes and immobilize them in a form in which substrates can be continually contacted with them, enzymes can be used repeatedly and enzymatic reaction processes can be carried out continuously. (google.com)
  • In this work, using benzydamine as a model drug, two approaches were tested and optimized for the synthesis of benzydamine N -oxide: an enzyme immobilization system using two HisTrap HP columns connected in series carrying His 6 -GDH and His 6 -FMO3 enzymes respectively, and a two-strain-mixed-culture strategy using cell suspensions of E. coli JM109 (containing a FMO3 gene) and BL21 (containing a GDH gene). (biomedcentral.com)
  • This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. (springer.com)
  • Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field. (springer.com)
  • Bio-fuel cells catalyzed by enzymes are expected to provide next-generation energy supply sources for microelectronic devices because they are safe to use and are light in weight and can be easily miniaturized [ 1 - 4 ]. (omicsonline.org)
  • Porous silicone rubber as an immobilization matrix for microbial and mammalian cells: natural immobilization of a mass transfer limited culture environment (A.J. Knights).Local mass transfer coefficients in bacterial biofilms using fluorescence recovery after photobleaching (FRAP) biofilms (J.D. Bryers, F. Drummond). (elsevier.com)
  • Subject matter wherein the enzyme or microbial cell is bonded to the polymeric carrier through an intermediate compound which attaches to both the enzyme and the carrier. (patentgenius.com)
  • Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. (bioportfolio.com)
  • fuel cells that use whole cells to catalyze fuel are called microbial fuel cells ). (wikipedia.org)
  • Early work with biofuel cells, which began in the early 20th century, was purely of the microbial variety. (wikipedia.org)
  • Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation. (sigmaaldrich.com)
  • The project encompasses complex solution of a bioremediation technology:microbial degradation of organic pollutants,whole cell immobilization, reactor design and environmental engineering enabling tailor-made bioremedial actions. (eurekanetwork.org)
  • Scanning electron microscopy was used to prove that immobilized photosensitizers excited by white light caused irreversible damage to microbial cells. (wiley.com)
  • The aim of the present study was to evaluate the production of sour milk fermented with the use of the probiotic microorganism Lactobacillus casei ATCC 393 immobilized on wheat bran. (omicsonline.org)
  • ARTICLE{Nühse03largescaleanalysis, author = {Thomas S. Nühse and Allan Stensballe and Ole N. Jensen and Scott C. Peck}, title = {Largescale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry}, journal = {Mol. (psu.edu)
  • To date, immobilized metal ion affinity chromatography (IMAC) for phos-phopeptides has shown great promise for large-scale studies, but has a reputation for poor specificity. (psu.edu)
  • Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity. (nih.gov)
  • We carried out phosphoproteomic analysis of rat inner medullary collecting duct cells by using a combination of phosphopeptide enrichment by immobilized metal affinity chromatography and phosphorylation site identification by liquid chromatography-mass spectrometry n neutral loss scanning. (pnas.org)
  • Recent studies have introduced two new innovations, namely, immobilized metal affinity chromatography (IMAC) ( 1 - 3 ) and liquid chromatography-mass spectrometry (LC-MS) 3 neutral loss scanning ( 1 - 4 ), to increase the efficiency of phosphopeptide identification. (pnas.org)
  • Limitations on biofuel production using cell culture (Escherichia coli, Clostridium, Saccharomyces cerevisiae, brown microalgae, blue-green algae and others) include low product (alcohol) concentrations (≤0.2 vol%) due to feedback inhibition, instability of cells, and lack of economical product recovery processes. (osti.gov)
  • Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. (biomedsearch.com)
  • The resultant syrup was then incubated with Saccharomyces cerevisiae L1 cells. (sigmaaldrich.com)
  • 1. The combination comprising a closed container, a water-containing beverage or food product contained in the container and dried Saccharomyces cerevisiae having about 94-96% dry matter immobilized in a solid material comprising a polymer acceptable for use in direct contact with beverages or food products for human consumption, the solid material containing the immobilized dried. (freepatentsonline.com)
  • d) removing oxygen from the closed container by action of the immobilized Saccharomyces cerevisiae being activated by water penetrating the solid material. (freepatentsonline.com)
  • Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). (elsevier.com)
  • E. coli cells containing overexpressed transaminases of various selectivities and hollow silica microspheres as supporting agent were immobilized by an improved sol-gel process to produce immobilized transaminase biocatalysts with suitable stability and mechanical properties for continuous-flow applications. (elsevier.com)
  • By using the most suitable immobilized transaminase biocatalysts, this study describes the first transaminase-based approach for the production of both pure enantiomers of 1-(3,4-dimethoxyphenyl)ethan-1-amine 1d. (elsevier.com)
  • The isomerization of glucose to fructose, the production of amino acids, and the hydrolysis of penicillins to 6-aminopenicillanic acid are some examples of the industrial processes, based on immobilized biocatalysts, that are operative at present. (springer.com)
  • These and other processes utilized in the food and the pharmaceutical industries, as well as a number of potentially useful immobilized biocatalysts, which have been investigated on pilot plant or laboratory scale, are discussed in this review. (springer.com)
  • 2016. Nanoscopy of bacterial cells immobilized by holographic optical tweezers. (uni-bielefeld.de)
  • abstract = "Immobilization of Candida rugosa cells on a solid support for extracellular lipase production has been explored. (uab.cat)
  • In addition, the production of biosurfactants by immobilized cells of Bacillus subtilis was well studied [13, 14]. (thefreedictionary.com)
  • The aim of the study was to determine optimal conditions for amylases biosynthesis by immobilized Bacillus polymyxa B-20 cells. (olsztyn.pl)
  • Hydrogen peroxide degradation by immobilized cells of alkaliphilic Bacillus halodurans. (ac.ke)
  • This fact demonstrates that the reactor system is limited in terms of organic substrate dispersion and direct contact with cells. (uab.cat)
  • The continuous enzymatic conversion of D-galactose to D-tagatose with an immobilized thermostable L-arabinose isomerase in packed-bed reactor and a novel method for D-tagatose purification were studied. (sigmaaldrich.com)
  • Biotrans- formation has been carried out in a fluidized bed reactor specially designed to obtain good contact conditions between cells entrapped into small calcium alginate beads (2-3 mm) and low-concentration acrylamide so- lutions (10~40 g 9 1-1). (inrae.fr)
  • Immobilized cell reactor. (speakerdeck.com)
  • Advanced 'Immobilized Cell Reactor' technology for treatment of wastewater. (speakerdeck.com)
  • Advanced 'Immobilized Cell Reactor' technology applied to leather industry. (speakerdeck.com)
  • Advanced 'Immobilized Cell Reactor' technology applied to textile industry. (speakerdeck.com)
  • CO2 absorption with immobilized CA was optimal with the enzyme load of 1mg and gas flow of 650mlmin−1at 30°C in vertical reactor. (deepdyve.com)
  • Continuous sucrose conversion into fructose and ethanol by immobilized cell reactor of fructose utilization-negative mutant of Zymomonas mobilis was investigated. (who.int)
  • 1 h the immobilized cell reactor of fructose utilization-negative mutant produced 57. (who.int)
  • An immobilized-cell membrane bioreactor was fabricated to investigate degradation of phenol at high concentrations using Pseudomonas putida American Type Culture Collection 49451. (nus.edu.sg)
  • Aerobic biodegradation of phenol by Immobilized Pseudomonas sp. (sciepub.com)
  • Fusion proteins of labile KdcA (fKdcA) were expressed to stabilize the covalently immobilized KdcA. (osti.gov)
  • The evidence was getting stronger that cells had evolved a fine-tuned mechanism for organizing some of their internal structure and processes through phase separation-that is, letting proteins self-assemble into structures that could perform distinct functions. (scientificamerican.com)
  • Organisms use reversible phosphorylation of proteins to control many cellular processes including signal transduction, gene expression, cell cycle, cytoskeletal regulation, and apoptosis. (mcponline.org)
  • To investigate the relationship between NF-κB and GM-CSF transcription in a chromatin context, we analyzed the chromatin structure of the GM-CSF gene in T cells and the role of NF-κB proteins in chromatin remodeling. (rupress.org)
  • These results suggest that chromatin remodeling across the GM-CSF promoter in T cells is a result of recruitment of SWI/SNF type remodeling complexes by NF-κB proteins binding to the CD28 response region of the promoter. (rupress.org)
  • When the researchers examined which genes were active in these cells, they found increased expression in molecular pathways relating to how the cells attach to surfaces and interact with the extracellular matrix, a network of proteins and other molecules outside cells that nurture them and provide physical support. (utsouthwestern.edu)
  • Anti-TMT Resin is agarose-immobilized anti-TMT monoclonal antibody, which is effective to capture and affinity purify peptides or proteins that have been labeled using any TMT Reagent. (fishersci.com)
  • Recent developments in MS have also enabled the quantitative analysis of protein phosphorylation on hundreds of proteins, information that has been correlated to cell fate in recent studies ( 4 - 6 ). (pnas.org)
  • Conventional tissue markers include cell morphology and metabolism, the presence of certain enzymic activities or proteins or other molecules of biological import, the accumulation or disappearance of such molecules, etc. (google.ca)
  • Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads. (biomedsearch.com)
  • PVA effectively improves the stability of immobilized cell beads with a promoted mechanical strength, while SA improves the surface properties of immobilized cell beads to keep from the tendency to agglomeration. (springeropen.com)
  • Guidelines for the characterization of immobilized cells (R.H. Wijffels et al. ). (elsevier.com)
  • Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given. (elsevier.com)
  • The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. (elsevier.com)
  • Ferrer, P & Solà, C 1992, ' Lipase production by immobilized Candida rugosa cells ', Applied Microbiology and Biotechnology , vol. 37, no. 6, pp. 737-741. (uab.cat)
  • 2. The process of claim 1 wherein the immobilized cells are coated onto beads or other particulate material. (google.ca)
  • Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-(dimethylphenylazo)-2-naphthol-6-sulfonic acid sodium salt in the laboratory. (mdpi.com)
  • The process was monitored by chemical and sensorial analyses and dominance of starters and cell leaking from beads were also checked. (frontiersin.org)
  • In contrast, the maximum rate of hydrolysis is considerably decreased, as if only cells on the outskirts of beads were involved in the transformation. (inrae.fr)
  • These results suggest that nanotopography affects cell adhesion, proliferation, as well as cell elongation, while collagen immobilized surfaces greatly affect cell differentiation. (mdpi.com)
  • Work in the 1980s by the McConnell and McCloskey laboratories ( 18 , 19 ) established that liposomes bearing haptens for IgE could mediate adhesion to rat basophilic leukemia 2H3 cell line (RBL-2H3) mast cells and provide lateral forces that induced both FcεRI clustering and serotonin release. (jimmunol.org)
  • In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment III8-10 coated on titanium versus a novel low-modulus TiNbHf alloy. (upc.edu)
  • The influence of the different bioactive coatings on the adhesion of rat mesenchymal stem cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell force spectroscopy. (upc.edu)
  • Our results show that bioactive moieties, particularly fibronectin fragment III8-10, improve cell adhesion on titanium and TiNbHf and that the covalent tethering of such molecules provides the most promising strategy to biofunctionalize these materials. (upc.edu)
  • Polyallylamine, which is cationic and enhances cell adhesion electrostatically (non-specifically), was co-immobilized with a cell growth factor, insulin, onto a surface-hydrolysed poly(methyl methacrylate) membrane. (semanticscholar.org)
  • Specific cell-cell recognition and adhesion may involve cell surface glycoconjugates on one cell binding the complementary carbohydrate receptors on an apposing cell surface. (elsevier.com)
  • This carbohydrate-directed cell adhesion may depend on cell surface carbohydrate receptors which mediate both the initial specific adhesion and complex postrecognition cellular responses. (elsevier.com)
  • While the relationship of the cell adhesion demonstrated here to cell-cell recognition in vivo has yet to be determined, this well-controlled biochemical approach may reveal new information on the way in which cells analyze and respond to their immediate external environment. (elsevier.com)
  • Cell adhesive peptides, GRGDS and YIGSR, were found to promote adhesion and spreading of ECs and showed a synergistic effect when both of them were used. (aminer.cn)
  • In vitro studies indicate that both adhesion to epithelial cells and auto aggregation are mostly mediated by type IV pili (Carbonnelle et al , 2006 ). (embopress.org)
  • The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. (frontiersin.org)
  • Cells co-entrapped together with polyurethane into Ca-alginate did not show higher lipase production levels than the cells entrapped in Ca-alginate gels. (uab.cat)
  • Optimization of olive oil hydrolysis process using immobilized Lipase from Burkholderia cepacia sp. (thefreedictionary.com)
  • The stability of free and immobilized lipase was determined by measuring the residual activity against various temperature ranging from 40-80[degrees]C. (thefreedictionary.com)
  • Be the first to review our Rabbit anti-Goat IgG (H L) Secondary Antibody [Agarose Immobilized] and receive a gift card or discount. (novusbio.com)
  • Covalently immobilized ADH exhibited long-term stability and efficient conversion of aldehyde to alcohol over multiple batch cycles without fusions. (osti.gov)
  • In this work, collagen IV was covalently immobilized to the surface of polyimide membranes, with the purpose of developing scaffold materials for RPE cell culture. (tut.fi)
  • The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. (degruyter.com)
  • Enzymatic biofuel cells, while currently confined to research facilities, are widely prized for the promise they hold in terms of their relatively inexpensive components and fuels, as well as a potential power source for bionic implants. (wikipedia.org)
  • A general diagram for an enzymatic biofuel cell using Glucose and Oxygen . (wikipedia.org)
  • Enzymatic biofuel cells work on the same general principles as all fuel cells: use a catalyst to separate electrons from a parent molecule and force it to go around an electrolyte barrier through a wire to generate an electric current. (wikipedia.org)
  • What makes the enzymatic biofuel cell distinct from more conventional fuel cells are the catalysts they use and the fuels that they accept. (wikipedia.org)
  • [1] Because sugars and other biofuels can be grown and harvested on a massive scale, the fuel for enzymatic biofuel cells is extremely cheap and can be found in nearly any part of the world, thus making it an extraordinarily attractive option from a logistics standpoint, and even more so for those concerned with the adoption of renewable energy sources . (wikipedia.org)
  • Enzymatic biofuel cells also have operating requirements not shared by traditional fuel cells. (wikipedia.org)
  • Previously, many researchers have developed enzymatic biofuel cells [ 5 - 13 ] that were shown to have current and power densities to some extent, but were limited by major issues including low output of the battery because of inefficient redox reaction on the electrode surface and lower active lifetime because of low stability of enzyme [ 14 , 15 ]. (omicsonline.org)
  • The immobilized cells were successfully reused for BDS over seven batch cycles but the uncoated free cells could be used only once. (thefreedictionary.com)
  • Moreover, in repeated-batch operations, immobilized Arthrobacter sp. (eugris.info)
  • In this study, we have developed collagen immobilized nanostructured surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of human aortic SMCs. (mdpi.com)
  • The nanowire surfaces were fabricated from polycaprolactone and were immobilized with collagen. (mdpi.com)
  • The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that SMCs on nanostructured surfaces may be differentiated and slowly dividing. (mdpi.com)
  • Scanning electron microscopy results reveal that SMCs on nanostructured surfaces are more elongated and that cells are interacting with the nano-features on the surface. (mdpi.com)
  • After providing differentiation cues, heavy chain myosin and calponin, specific to a contractile SMC phenotype, are upregulated on collagen immobilized surfaces. (mdpi.com)
  • Rat basophilic leukemia mast cells primed with fluorescent anti-DNP IgE were engaged by surfaces presenting either bilayer-incorporated, monovalent DNP-lipid (mobile ligand), or chemically cross-linked, multivalent DNP (immobilized ligand). (jimmunol.org)
  • In this study, we explore the potential for mast cells to form synapses when presented with Ag on surfaces, analogous to the responses of T cells and B cells. (jimmunol.org)
  • To overcome this problem, newly modified surfaces were designed and evaluated for the cell-rolling surface. (nii.ac.jp)
  • Hydrophilic polymer grafting, silane coupling reagents, and SAM on Au were selected and evaluated for the cell-rolling surfaces. (nii.ac.jp)
  • Such interactions have been modeled by immobilizing simple synthetic glycosides, glycoproteins, glycosaminoglycans, and glycolipids on otherwise inert plastic surfaces and incubating them with intact cells. (elsevier.com)
  • Gene delivery by non-specific adsorption of non-viral vectors to protein-coated surfaces can reduce the amount of DNA required, and also increase transgene expression and the number of cells expressing the transgene. (pubmedcentralcanada.ca)
  • Covalently modified surfaces showed cell attachment and cell properties comparable to the uncoated and dip-coated controls. (tut.fi)
  • Viability of immobilized cells: use of specific ATP levels and oxygen uptake rates (P. Gikas, A.G. Livingston). (elsevier.com)
  • Cell viability began to decline on day 5 ofculture. (portlandpress.com)
  • In the present study, a recombinant E. coli was immobilized using hollow fiber membrane to improve the enzyme excretion, cell viability and plasmid stability. (ump.edu.my)
  • Accordingly, the following objectives were set: i) to evaluate the impact of low-intensity radiation on cell suspensions, and ii) to carry out a comparative analysis of the viability of cells immobilized in porous TiNi-based alloy and IR- and UV-irradiated. (vbripress.com)
  • The data show that the extracellular environment of bone marrow, tumor and spleen cell populations affects their viability and proliferative potency in porous TiNi-based scaffolds. (vbripress.com)
  • IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, tumor, and spleen cells. (vbripress.com)
  • In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell proliferation activity. (vbripress.com)
  • These data indicate that FcεRI cross-linking is not an obligatory step in triggering mast cell signaling and suggest that dense populations of mobile receptors are capable of initiating low-level degranulation upon ligand recognition. (jimmunol.org)
  • Ligand binding to cell surface receptors activates multiple protein tyrosine phosphorylation-mediated signaling cascades that regulate many cell biological processes, including proliferation, differentiation, migration, and cell death ( 1 - 3 ). (pnas.org)
  • Photosensitizers immobilized on a solid phase can be applied for continuous disinfection of wastewater bacteria. (wiley.com)
  • In the immobilized system, it was observed that cells diffused from the membranes when phenol concentrations reached noninhibitory levels in a few experiments. (nus.edu.sg)
  • This work demonstrated the potential of collagen IV-immobilized polyimide membranes as substrates for the growth of ARPE-19 cells. (tut.fi)
  • 1. A process for preparing phenylalanine which comprises preparing an aqueous reaction mixture consisting essentially of phenylpyruvic acid or phenylpyruvate, pyridoxal -5-phosphate as a cofactor and an amine donor selected from the group consisting of L-glutamic acid and L-aspartic acid, and contacting said reaction mixture with cells immobilized with a polyazetidine polymer, the cells having transaminase activity to obtain said phenylalanine in high yield. (google.ca)
  • Production of glutamic acid using whole and immobilized cells of Corynebacterium glutamicum. (thefreedictionary.com)
  • Comparatively, whole cells produced more glutamic acid than immobilized cells. (thefreedictionary.com)
  • On the other hand, cells immobilized in 25% by weight polysulfone fibers degraded phenol at concentrations above 1,000 mg/L. At an initial phenol concentration of 1,200 mg/L, phenol was fully degraded within 95 h in the immobilized system, whereas no cell growth and phenol degradation were observed in the free suspension system at 1,000 mg/L phenol. (nus.edu.sg)
  • In such cases, the time taken for complete degradation was shorter with cell diffusion because suspension cells were responsible for the rapid phenol degradation. (nus.edu.sg)
  • Environmental Expert.com/Microbiological degradation of pentane by immobilized cells of Arthrobacter sp. (eugris.info)
  • Year: 2005 Title: Microbiological degradation of pentane by immobilized cells of Arthrobacter sp. (eugris.info)
  • cells were able to maintain 85-95% pentane degradation during a 2month period. (eugris.info)
  • Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB , gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were immobilized onto the polystyrene surface of the wells of microtiter plates using adsorption method, followed by cross-linking with glutaraldehyde. (thescipub.com)
  • The method involved culturing cells with the expanded polystyrene in shake-flasks, followed by in situ growth within the column. (eugris.info)
  • Methylene blue (MB) and Rose Bengal were immobilized in polystyrene by mixing solutions of the photosensitizers in chloroform with a polymer solution, followed by air evaporation of the solvent. (wiley.com)
  • The method proposed in this work is based on conversion of the waste glycerol to dihydroxyacetone in the reaction catalyzed by an immobilized cell extract with the activity of glycerol dehydrogenase, produced from Gluconobacter oxydans bacteria and it could be an effective way to utilize the waste glycerol into a valuable product. (ejbiotechnology.info)
  • The antimicrobial activity of the immobilized photosensitizers was tested against Gram-positive and Gram-negative bacteria. (wiley.com)
  • The membrane bioreactor also showed 2.5-fold increase in the CGTase excretion (473 x 103 U/ml) with 75% reduction of cell lysis compared to shake flask culture (190 x 103 U/ml of CGTase activity). (ump.edu.my)
  • Non-covalent immobilized artificial membrane reverse-phase high performance liquid chromatography was previously evaluated as a means whereby elution times for antimicrobial peptides from columns mimicking the lipid bilayers of different membrane systems might be used as a fast-screening method to compare relative binding effectiveness. (ualberta.ca)
  • The objective of this study is to reveal how SMCs interact with collagen immobilized nanostructures. (mdpi.com)
  • In contrast, collagen fibers in the immobilized animals had a more random pattern, restricting the MSC stretch, keeping them compact. (utsouthwestern.edu)
  • When the researchers placed MSCs on aligned collagen in lab dishes, they eventually morphed into bone cells. (utsouthwestern.edu)
  • Gene activation patterns in both groups of cells confirmed increased expression of bone or fat genes, depending on whether they grew on aligned or unaligned collagen matrices, respectively. (utsouthwestern.edu)
  • The immobilized cells exhibited favourable operational stability. (springer.com)
  • This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and screening with the advantages of simplicity, stability, ease and convenience for assembly and use, and low cost. (thescipub.com)
  • The effects of different polymers of hollow fiber membrane and culture conditions on the cyclodextrin glucanotransferase (CGTase) excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. (ump.edu.my)
  • The immobilized cells on a polyvinylidene fluoride polymer exhibited a 2.0-4.5-fold increase in the CGTase excretion with 18-95% reduction of cell lysis and over 100% increment of plasmid stability compared to the free cells. (ump.edu.my)
  • The use of low tryptone concentration (5 g/1) reduced the occurrence of cell lysis (90% reduction) and increased the plasmid stability (86% increment) without significant change in CGTase excretion in comparison with initial tryptone concentration (20 g/1). (ump.edu.my)
  • Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface. (biomedsearch.com)
  • A series of new substrates, β-phenylserine, β-(nitrophenyl) serine and β-(methylsulfonylphenyl) serine were used in the resolution process catalyzed by immobilized Escherichia coli cells with SHMT activity. (springer.com)
  • The effect of variation the substrate's temperature on the free and immobilized [beta]-galactosidase activities were investigated, by using lactose as substrates, and the obtained data are shown in Figure 4. (thefreedictionary.com)
  • Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. (elsevier.com)
  • In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity. (elsevier.com)
  • However, cell lysis is one of the major drawbacks during the excretion of recombinant enzyme when using Escherichia coli (E. coli) as a host. (ump.edu.my)
  • Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles. (degruyter.com)
  • All fermenta- tions with immobilized yeast cells had a shorter lag phase and faster utilization of sugars and ethanol production than those fermented with suspended cells. (vtt.fi)
  • In this study, we show that receptor reorganization within the contacting mast cell membrane is markedly different upon binding of mobile and immobilized ligands. (jimmunol.org)
  • The effects of pH and temperature on D-tagatose production reaction catalyzed by free and immobilized TMAI were investigated. (sigmaaldrich.com)
  • The optimum reaction yield with immobilized TMAI increased by four percentage points to 43.9% compared with that of free TMAI. (sigmaaldrich.com)
  • By immobilizing the enzyme with orientation, the accessibility between the enzyme and substrate for enzyme reaction increased because the active site of PQQ-GDH is located opposite the electrode. (omicsonline.org)
  • The principle of bio-fuel cells is based on the redox reaction between the enzyme and substrate. (omicsonline.org)
  • We are interested in the external and internal cues that regulate mammalian stem cell division and cell fate choice. (kcl.ac.uk)
  • MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. (nature.com)
  • A plausible explanation for the lack of agreement could be the different sources of BRAK used by investigators, which included a synthetic polypeptide, a murine homologue, and unpurified conditioned supernatants from transfected mammalian cells. (aacrjournals.org)
  • This study reveals the practicality of liquid chromotography-mass spectrometry n neutral loss scanning for large-scale identification and quantification of protein phosphorylation in the analysis of cell signaling in a native mammalian system. (pnas.org)
  • Quantification of phosphopeptides in this setting has been challenging and has been successful so far in cultured cells ( 5 ) and yeast ( 6 ) but not in native mammalian cells and tissues. (pnas.org)
  • A common use of immobilized cells is in biotechnology for the bioconversion of a substrate to a particular product. (bioportfolio.com)
  • First, transfected cell arrays are based on the parallel delivery of DNA from a substrate, and enable high-throughput studies on gene function or activity [ 9 , 12 , 13 ]. (pubmedcentralcanada.ca)
  • The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. (elsevier.com)
  • A process is disclosed for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. (google.ca)
  • The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed. (google.ca)
  • 12. A composition comprising immobilized cells having transmaminase activity, an amine donor, and phenylpyruvic acid or phenylpyruvate, said cells being immobilized with a polyazetidine polymer. (google.ca)
  • 13. A composition suitable for use in converting phenylpyruvic acid or phenylpyruvate to phenylalanine comprising immobilized cells having transaminase activity, said cells being immobilized with a polyazetidine polymer. (google.ca)
  • Nitrification activity of immobilized activated sludge evaluated by respiration rate (H. Nakamura et al. ). (elsevier.com)
  • Genome-wide transcriptional analysis shows that complex 2 exerts its anticancer activity through metabolism repression and multiple cell death signalling pathways. (rsc.org)
  • Deacidification activity of immobilized yeast cells was maintained for at least 20 months after their encapsulation, at which point they were stored at 4°C. Alginate bead quality tests showed that immobilized cells could be recycled up to five times without liberation of yeast cells into wine must. (ajevonline.org)
  • The uricase activity of immobilized cells had a value of KM and [V.sub.max], respectively of 2.86 mM and 0.0018 mA. (thefreedictionary.com)
  • In addition, the catalytic activity of immobilized enzyme at high temperature and low pH was significantly improved compared with free enzyme. (sigmaaldrich.com)
  • Tengholm, A., Teruel, M.N. & Meyer, T. Single cell imaging of PI3K activity and glucose transporter insertion into the plasma membrane by dual color evanescent wave microscopy. (nature.com)
  • It appears that arginase activity played an important role in H 2 O 2 accumulation in these cells. (jimmunol.org)
  • Nessler ammonia assay method was used for the determination of the urease activity of both free and immobilized enzyme [20]. (thefreedictionary.com)
  • The immobilized microorganism was able to actively degrade a 10% mixture of pentane, allowing us to obtain a bioconversion yield of 90% after 36h. (eugris.info)
  • 16. A process for preparing phenylalanine which comprises contacting cinnamic acid with immobilized cells having phenylalanine ammonia lyase actibity, said cells being immobilized with a polyazetidine polymer. (google.ca)
  • Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells. (sigmaaldrich.com)
  • However, conventional IMAC adsorbents with iminodiacetic acid as the chelating group to immobilize Fe 3+ lack enough specificity for efficient phosphoproteome analysis. (mcponline.org)
  • Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. (google.com)
  • The ICAT quantitative labeling strategy (3, 4) is perhaps the best-characterized method for relative protein quantitation using MS. Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine (5), lysine (6), tyrosine (7), and leucine (8), for in vivo incorporation of a mass difference to support relative quantitation. (psu.edu)
  • Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. (google.ca)
  • To overcome these challenges, an alternate simplified biofuel production scheme was tested based on a cell-free immobilized enzyme system. (osti.gov)
  • Using this cell free system, we were able to obtain about 2.6 times higher concentrations of iso-butanol using our non-optimized system as compared with live cell systems. (osti.gov)
  • This integrated biocatalytic system is able to perform simultaneously AF and MLF, producing wines similar in organoleptic traits in comparison with wines fermented following traditional sequential AF and MLF with free cell starters. (frontiersin.org)
  • Through phase separation, they spontaneously self-assemble into dynamic, membrane-free, dropletlike structures that can perform needed tasks in cells . (scientificamerican.com)
  • The biota of all three beverages was subsequently supplemented with free and immobilized cells of B. (thefreedictionary.com)
  • Figure 4 illustrates the growth curves for free and immobilized cells of Nannochloropsis sp. (thefreedictionary.com)
  • when assayed either as free or immobilized cells showed chlorpyrifos removal percentages of 40.17% and 71.05%, respectively, and for pentachlorophenol 5.24% and 14.72%, respectively, suggesting better removal of both pesticides by using immobilized cells. (hindawi.com)
  • These results reveal that environments contaminated with mixtures of xenobiotics could be successfully cleaned up by using either free or immobilized cultures of Streptomyces , through in situ or ex situ remediation techniques. (hindawi.com)
  • The growth and metabolic profiles were evaluated for both free and immobilized cells. (comu.edu.tr)
  • Culture media with fruit waste produced good growth of free as well as immobilized yeast cells. (comu.edu.tr)
  • In this study we asked whether ImC from tumor-free and tumor-bearing hosts differ in their ability to suppress CD8 + T cells, and what could be a mechanism of Ag-specific inhibition of T cell response by ImC in cancer. (jimmunol.org)
  • The effects of optimum temperature on the free and immobilized enzyme were determined by assaying their activities at different temperatures (0 to 80[degrees]C) in 50 mM sodium phosphate (pH 7. (thefreedictionary.com)
  • It appears that the Michaelis constant does not change with operating conditions and remains roughly equal to the value obtained for free cells. (inrae.fr)
  • Better results were observed in effluent sample remediated with immobilized cells rather than free cells. (mahendrapublications.com)
  • The cells are preferably immobilized with a polyazetidine polymer. (google.ca)
  • 17. The process of claim 16 wherein the immobilized cells are Rhodotorula glutinis cells and wherein the polyazetidine polymer is coated onto a particulate support. (google.ca)
  • Plasma polymer and PEG-based coatings for DNA, protein and cell microarrays. (biomedsearch.com)
  • A phosphorylcholine-modified chitosan polymer as an endothelial progenitor cell supporting matrix. (semanticscholar.org)
  • To give new insight into the intracellular trafficking of NPs that target the CD44 receptor, which is overexpressed on the surface of a broad variety of cancer cells, we have synthesized an NP pH sensor system that targets CD44. (sigmaaldrich.com)
  • The pH distribution profile in cells was measured for nanosensors with HA, cationic, and noncharged NP surface coatings giving a clear indication of the intracellular pH environment that the different NPs experience after internalization. (sigmaaldrich.com)
  • Scanning electron microscope and cell count analyses revealed highly dense, rod shaped bacterial cells stably attached to the well surface. (thescipub.com)
  • We successfully developed a stem cell rolling column with a soft-interface bearing anti cell surface marker molecules antibody. (nii.ac.jp)
  • As the results, zwitterionic telomer brush containing 2-methacryloyloxyethyl phosphorylcholine (MPC) efficiency suppressed the non-specific interaction between cells and antibody-immobilized surface. (nii.ac.jp)
  • 4. The method of claim 1 , wherein the device comprises a plurality of welds for maximizing surface area to volume ratio of the human PDX1-positive pancreatic progenitor cell population in the device. (google.es)
  • The first strategy was to increase the orientation of the enzyme immobilized onto the electrode surface. (omicsonline.org)
  • Scale up of immobilized-cell reactors (J.J. Heijnen). (elsevier.com)
  • In cell transplantation, alginate immobilized cells are utilized to act as the barrier between the host immune system and the transplanted cell [4]. (thefreedictionary.com)
  • Alginate immobilized cells have been used in cell transplantation where it acts as the barrier between transplant ( immobilized cell ) and host immune system. (thefreedictionary.com)
  • L-arabinose isomerase from Thermoanaerobacter mathranii (TMAI) was recombinantly overexpressed and immobilized in calcium alginate. (sigmaaldrich.com)
  • Encapsulation of the cells was performed by droplet formation from a foamed alginate solution. (comu.edu.tr)
  • This publication contains full papers of both oral and poster presentations of the symposium 'Immobilized Cells: Basics and Applications' that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. (elsevier.com)
  • Kinetic constants for the immobilized enzyme have been identified. (inrae.fr)
  • The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase , as well as its kinetic parameters (K M and Vmax). (bvsalud.org)
  • Most organic compounds cannot be used as fuel by fuel cells with metal catalysts because the carbon monoxide formed by the interaction of the carbon molecules with oxygen during the fuel cell's functioning will quickly "poison" the precious metals that the cell relies on, rendering it useless. (wikipedia.org)
  • [2] These difficulties led to an abandonment by biofuel cell researchers of the enzyme-catalyst model for nearly three decades in favor of the more conventional metal catalysts (principally platinum), which are used in most fuel cells. (wikipedia.org)
  • Polyurethane (PU) is widely used as a cardiovascular biomaterial due to its good mechanical properties and hemocompatibility, but it is not adhesive to endothelial cells (ECs). (aminer.cn)
  • Sheet of Colloidal Crystals Immobilized in Resin, Method of Displaying Structural Color Using Same, Method for. (patents.com)
  • A sheet of colloidal crystals immobilized in resin exhibiting intense structural color and allowing easy observation thereof from squarely facing direction. (patents.com)
  • Is the Subject Area "Endothelial cells" applicable to this article? (plos.org)
  • GM-CSF is induced in a variety of cell types, including T cells, macrophages, endothelial cells, and fibroblasts in response to immune and inflammatory signals ( 1 - 3 ). (rupress.org)