BooksAllied Health Occupations: Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.Library Collection Development: Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.Textbooks as Topic: Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.Book SelectionPhenylpyruvic Acids: A group of compounds that are derivatives of phenylpyruvic acid which has the general formula C6H5CH2COCOOH, and is a metabolite of phenylalanine. (From Dorland, 28th ed)Phenylalanine Ammonia-Lyase: An enzyme that catalyzes the deamination of PHENYLALANINE to form trans-cinnamate and ammonia.Phenylalanine: An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.Anabaena variabilis: A species of ANABAENA that can form SPORES called akinetes.Phenylalanine Hydroxylase: An enzyme of the oxidoreductase class that catalyzes the formation of L-TYROSINE, dihydrobiopterin, and water from L-PHENYLALANINE, tetrahydrobiopterin, and oxygen. Deficiency of this enzyme may cause PHENYLKETONURIAS and PHENYLKETONURIA, MATERNAL. EC 1.14.16.1.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Antilymphocyte Serum: Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.Antigens, Viral: Substances elaborated by viruses that have antigenic activity.Idoxuridine: An analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent.Epstein-Barr Virus Nuclear Antigens: Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.Optical Tweezers: A technique that uses LASERS to trap, image, and manipulate small objects (biomolecules, supramolecular assembles, DENDRIMERS) in three dimensional space. (From Glossary of Biotechnology and Nanobiotechnology Terms, 4th ed.)Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.Potoroidae: A family of rat kangaroos found in and around Australia. Genera include Potorous and Bettongia.Micromanipulation: The performance of dissections, injections, surgery, etc., by the use of micromanipulators (attachments to a microscope) that manipulate tiny instruments.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Drug Carriers: Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Cells, Immobilized: Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. A common use of immobilized cells is in biotechnology for the bioconversion of a substrate to a particular product. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Drug Delivery Systems: Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.Bacteriological Techniques: Techniques used in studying bacteria.Yeasts: A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.SwedenNanowires: Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.Vitallium: An alloy of 60% cobalt, 20% chromium, 5% molybdenum, and traces of other substances. It is used in dentures, certain surgical appliances, prostheses, implants, and instruments.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.Polyesters: Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Arthrobacter: A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.Pentanes: Five-carbon saturated hydrocarbon group of the methane series. Include isomers and derivatives.Polystyrenes: Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.Demeclocycline: A TETRACYCLINE analog having a 7-chloro and a 6-methyl. Because it is excreted more slowly than TETRACYCLINE, it maintains effective blood levels for longer periods of time.Glycine Hydroxymethyltransferase: A pyridoxal phosphate enzyme that catalyzes the reaction of glycine and 5,10-methylene-tetrahydrofolate to form serine. It also catalyzes the reaction of glycine with acetaldehyde to form L-threonine. EC 2.1.2.1.Fructose-Bisphosphate Aldolase: An enzyme of the lyase class that catalyzes the cleavage of fructose 1,6-biphosphate to form dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. The enzyme also acts on (3S,4R)-ketose 1-phosphates. The yeast and bacterial enzymes are zinc proteins. (Enzyme Nomenclature, 1992) E.C. 4.1.2.13.Threonine Dehydratase: A pyridoxal-phosphate protein that catalyzes the deamination of THREONINE to 2-ketobutyrate and AMMONIA. The role of this enzyme can be biosynthetic or biodegradative. In the former role it supplies 2-ketobutyrate required for ISOLEUCINE biosynthesis, while in the latter it is only involved in the breakdown of threonine to supply energy. This enzyme was formerly listed as EC 4.2.1.16.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Iridium: A metallic element with the atomic symbol Ir, atomic number 77, and atomic weight 192.22.Spectroscopy, Electron Energy-Loss: A technique for analysis of the chemical composition of molecules. A substance is bombarded with monochromatic ELECTRONS. Some of the electrons passing through the specimen will lose energy when they ionize inner shell electrons of the atoms in the specimen. The energy loss is element dependent. Analysis of the energy loss spectrum reveals the elemental composition of a specimen. ENERGY-FILTERED TRANSMISSION ELECTRON MICROSCOPY is a type of electron energy loss spectroscopy carried out in electron microscopes specially outfitted to analyze the spectrum of electron energy loss.Glycolysis: A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Cell Line, Tumor: A cell line derived from cultured tumor cells.Chemical EngineeringMicroalgae: A non-taxonomic term for unicellular microscopic algae which are found in both freshwater and marine environments. Some authors consider DIATOMS; CYANOBACTERIA; HAPTOPHYTA; and DINOFLAGELLATES as part of microalgae, even though they are not algae.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Technical Report

Immobilization patterns and dynamics of acetate-utilizing methanogens immobilized in sterile granular sludge in upflow anaerobic sludge blanket reactors. (1/189)

Sterile granular sludge was inoculated with either Methanosarcina mazeii S-6, Methanosaeta concilii GP-6, or both species in acetate-fed upflow anaerobic sludge blanket (UASB) reactors to investigate the immobilization patterns and dynamics of aceticlastic methanogens in granular sludge. After several months of reactor operation, the methanogens were immobilized, either separately or together. The fastest immobilization was observed in the reactor containing M. mazeii S-6. The highest effluent concentration of acetate was observed in the reactor with only M. mazeii S-6 immobilized, while the lowest effluent concentration of acetate was observed in the reactor where both types of methanogens were immobilized together. No changes were observed in the kinetic parameters (Ks and mumax) of immobilized M. concilii GP-6 or M. mazeii S-6 compared with suspended cultures, indicating that immobilization does not affect the growth kinetics of these methanogens. An enzyme-linked immunosorbent assay using polyclonal antibodies against either M. concilii GP-6 or M. mazeii S-6 showed significant variations in the two methanogenic populations in the different reactors. Polyclonal antibodies were further used to study the spatial distribution of the two methanogens. M. concilii GP-6 was immobilized only on existing support material without any specific pattern. M. mazeii S-6, however, showed a different immobilization pattern: large clumps were formed when the concentration of acetate was high, but where the acetate concentration was low this strain was immobilized on support material as single cells or small clumps. The data clearly show that the two aceticlastic methanogens immobilize differently in UASB systems, depending on the conditions found throughout the UASB reactor.  (+info)

Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (2/189)

Viable cells of Pseudomonas aeruginosa were entrapped in alginate gel layers and incubated in a minimal glucose (15 g/L)-yeast extract (2 g/L)-salt medium to form artificial biofilm-like structures. After cultivation for 2 days, the biomass distribution inside the polymer was highly heterogeneous. The cell number reached approximately 1011 cells/g gel in the outer regions of the gel structures whereas the inner areas were less colonized (c. 10(8) cells g/gel). Killing of immobilized organisms by imipenem and tobramycin were compared with free-cell experiments (inoculum c. 10(9) cells/mL). Sessile-like bacteria displayed a higher resistance to the two antibiotics used alone or in combination than did suspended cells. Exposure for 10 h to 20 x MIC imipenem and 15 x MIC tobramycin reduced the number of viable immobilized bacteria to 0.3% and 3%, respectively, of the initial cell population, whereas these antibiotic concentrations were much more efficient (bactericidal) against free-cell cultures (5 log kill in 6 h). A synergic effect of tobramycin and imipenem was detected on bacterial suspensions but not on biofilm-like structures. Effective diffusivity measurements showed that the diffusion of imipenem in the alginate layer was not hindered. A slight but significant enhancement of beta-lactamase induction in immobilized cells as compared with their suspended counterparts was insufficient to explain the high resistance of sessile-like bacteria.  (+info)

Distribution of Nitrosomonas europaea and Paracoccus denitrificans immobilized in tubular polymeric gel for nitrogen removal. (3/189)

To improve the cooperative removal of nitrogen by Nitrosomonas europaea and Paracoccus denitrificans, we controlled their distribution in a tubular gel. When ethanol was supplied inside the tubular gel as an electron donor, their distributions overlapped in the external region of the gel. By changing the electron donor from ethanol to gaseous hydrogen, the distribution of P. denitrificans shifted to the inside of the tube and was separated from that of N. europaea. The separation resulted in an increase of the oxidation rate of ammonia by 25%.  (+info)

Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. (4/189)

Bifidobacterium longum KCTC 3128 and HLC 3742 were independently immobilized (entrapped) in calcium alginate beads containing 2, 3, and 4% sodium alginate. When the bifidobacteria entrapped in calcium alginate beads were exposed to simulated gastric juices and a bile salt solution, the death rate of the cells in the beads decreased proportionally with an increase in both the alginate gel concentration and bead size. The initial cell numbers in the beads affected the numbers of survivors after exposure to these solutions; however, the death rates of the viable cells were not affected. Accordingly, a mathematical model was formulated which expressed the influences of several parameters (gel concentration, bead size, and initial cell numbers) on the survival of entrapped bifidobacteria after sequential exposure to simulated gastric juices followed by a bile salt solution. The model proposed in this paper may be useful for estimating the survival of bifidobacteria in beads and establishing optimal entrapment conditions.  (+info)

Identification of a novel glycoprotein-binding activity in Streptococcus pyogenes regulated by the mga gene. (5/189)

The interaction between Streptococcus pyogenes and the host cell surface is not completely understood. Characterization of the adhesion mechanisms of the bacterium to the host cell surface is needed in order to develop new vaccines and anti-adhesion drugs. The presence of glycoprotein-binding activities among streptococcal strains was investigated. An activity binding to thyroglobulin, fetuin, asialofetuin and mucin but not non-glycosylated proteins was found to be present in the majority of the S. pyogenes strains studied. Cross-inhibition experiments suggested that the glycoproteins share a common structure recognized by the bacteria. The glycoprotein-binding activity was found to be proteinaceous, tightly attached to the bacterial surface and it also mediated the adherence of bacteria to solid surfaces coated with glycoproteins. The activity was found by transposon mutagenesis and complementation to be regulated by the multiple-gene regulator Mga, which has been implicated as a regulator of S. pyogenes virulence factors.  (+info)

Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface. (6/189)

Adhesion of Candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. C. albicans cells also adhere to several species of oral streptococci including Streptococcus gordonii, Streptococcus oralis and Streptococcus sanguinis in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of C. albicans cells by up to 2-3-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which C. albicans cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of S. gordonii cells but not to S. oralis cells. Parotid saliva that was pre-adsorbed with S. gordonii cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of C. albicans compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. C. albicans cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of C. albicans to S. gordonii cells. The results demonstrate that C. albicans cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote C. albicans adhesion and oral colonization.  (+info)

Response kinetics of tethered Rhodobacter sphaeroides to changes in light intensity. (7/189)

Rhodobacter sphaeroides can swim toward a wide range of attractants (a process known as taxis), propelled by a single rotating flagellum. The reversals of motor direction that cause tumbles in Eschericia coli taxis are replaced by brief motor stops, and taxis is controlled by a complex sensory system with multiple homologues of the E. coli sensory proteins. We tethered photosynthetically grown cells of R. sphaeroides by their flagella and measured the response of the flagellar motor to changes in light intensity. The unstimulated bias (probability of not being stopped) was significantly larger than the bias of tethered E. coli but similar to the probability of not tumbling in swimming E. coli. Otherwise, the step and impulse responses were the same as those of tethered E. coli to chemical attractants. This indicates that the single motor and multiple sensory signaling pathways in R. sphaeroides generate the same swimming response as several motors and a single pathway in E. coli, and that the response of the single motor is directly observable in the swimming pattern. Photo-responses were larger in the presence of cyanide or the uncoupler carbonyl cyanide 4-trifluoromethoxyphenylhydrazone (FCCP), consistent with the photo-response being detected via changes in the rate of electron transport.  (+info)

Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system. (8/189)

A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain GCH1 grew on propachlor with a generation time of 4.2 h and a rate of substrate utilization of 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but was subject to catabolite expression. Acetanilide was a growth substrate with a yield of 0.56 +/- 0.02 mg of protein micromol(-1). GCH1 strain cells were immobilized by adsorption onto a ceramic support and were used as biocatalysts in an immobilized cell system. Propachlor elimination reached 98%, with a retention time of 3 h and an initial organic load of 0.5 mM propachlor. The viability of immobilized cells increased 34-fold after 120 days of bioreactor operation.  (+info)

*Immobilized Cell Technology

... is a method of air filtration and purification that uses whole cell immobilization. It is a process ... Oxidation through immobilized cell technology is 12 times more efficient than natural oxidation is under the same conditions. ... As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that ... The key difference between traditional air filtration systems and those that employ immobilized cell technology is that the ...

*Immobilized whole cell

... in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required ... The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is ... However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. Zaushitsyna, O.; ... Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience.This is only ...

*Streptomyces avellaneus

Wijffels, edited by René H. (2001). Immobilized Cells. Berlin, Heidelberg: Springer Berlin Heidelberg. ISBN 3-642-56891-2. CS1 ... proceedings of a satellite symposium of the 3rd International Congress on Cell Biology, Gifu, Japan, 22-24 August 1984. Utrecht ...

*Arthrobacter globiformis

ISBN 3-662-06285-2. CS1 maint: Extra text: authors list (link) al.], edited by R.H. Wijffels ... [et (1996). Immobilized Cells ...

*Arthrobacter citreus

Karigar, C; Mahesh, A; Nagenahalli, M; Yun, DJ (February 2006). "Phenol degradation by immobilized cells of Arthrobacter ... "Phenol degradation by immobilized cells of Arthrobacter citreus". Biodegradation. 17 (1): 47-55. doi:10.1007/s10532-005-3048-y ...

*Lipase

"Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support ... As biological membranes are integral to living cells and are largely composed of phospholipids, lipases play important roles in ... Thus, some lipase activities are confined to specific compartments within cells while others work in extracellular spaces. In ... Spiegel S; Foster D; R Kolesnick (1996). "Signal transduction through lipid second messengers". Current Opinion in Cell Biology ...

*Microbial fuel cell

"Biochemical cells utilizing immobilized cells of Clostridium butyricum". Biotechnology and Bioengineering. 19 (11): 1727-1733. ... A microbial fuel cell (MFC), or biological fuel cell, is a bio-electrochemical system that drives an electric current by using ... 1) Microbial fuel cells use inorganic mediators to tap into the electron transport chain of cells and channel electrons ... Most microbial cells are electrochemically inactive. Electron transfer from microbial cells to the electrode is facilitated by ...

*Laminaria

Nestle, Nikolaus F.E.I.; Kimich, Rainer (March 1996). "NMR Imaging of Heavy Metal Absorption in Alginate, Immobilized Cells, ... These structures are very similar to mesophyll cells found in higher plant leaves. A laminaria stick may be used to slowly ...

*Biological photovoltaics

"A photoelectrochemical cell using immobilized photosynthetic membranes". Bioelectrochemistry and Bioenergetics. 22 (3): 391-401 ... Like other fuel cells, biological photovoltaic systems are divided into anodic and cathodic half-cells. Oxygenic photosynthetic ... The insulating outer membranes of whole cells impedes electron transfer from the sites of electron generation inside the cell ... and are sometimes also called photo-microbial fuel cells or "living solar cells". In a biological photovoltaic system, ...

*Arthrobacter rhombi

Elangovan, R.; Philip, Ligy; Chandraraj, K. (23 January 2009). "Hexavalent Chromium Reduction by Free and Immobilized Cell-free ...

*Pseudevernia furfuracea

Blanch M, Blanco Y, Fontaniella B, Legaz ME, Vicente C (2001). "Production of phenolics by immobilized cells of the lichen ...

*Pramod Tandon

"Manipulation of culture strategies to enhance capsaicin biosynthesis in suspension and immobilized cell cultures of Capsicum ... Tandon has worked on biotechnological enhancement of Capsaicin biosynthesis in cell cultures of Capsicum chinense (Naga King ...

*Electrophysiology

BERA is used primarily in biosensor applications in order to assay analytes that can interact with the immobilized cells by ... This technique is used to study how a cell responds when electric current enters a cell; this is important for instance for ... However, this invasive setup reduces the life of the cell and causes a leak of substances across the cell membrane. ... Instead of positioning a pipette on an adherent cell, cell suspension is pipetted on a chip containing a microstructured ...

*Bovine serum albumin

Zhang M, Desai T, Ferrari M (May 1998). "Proteins and cells on PEG immobilized silicon surfaces" (PDF). Biomaterials. 19 (10): ... BSA is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes ... During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often ... "Bovine serum albumin adsorption onto immobilized organotrichlorosilane surface: influence of the phase separation on protein ...

*Streptomyces varsoviensis

"Continuous Production of Oxytetracycline in Fluidized Bed Bioreactor by Immobilized Cells of Streptomyces varsoviensis MTCC- ...

*Industrial dye degradation

Immobilized fungal cells are more resistant to environmental stress and cells can be used repeatedly. Many dyes, specifically ... Kinetic Study of an Immobilized Iron Oxide for Catalytic Degradation of Azo Dye Reactive Black B with Catalytic Decomposition ...

*RBMX

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207 . PMID 23870131. "Infection and Immunity Immunophenotyping ( ... "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ...

*KIRREL

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell. Biol. 21 (14): 4829-36. doi:10.1128/MCB.21.14.4829-4836.2001. PMC 87176 . PMID 11416156. Huber TB, Schmidts M, Gerke P, ... cells involved in ensuring size- and charge-selective ultrafiltration of blood (Sellin et al., 2003). [supplied by OMIM] KIRREL ...

*RBM3

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... "Cell atlas - RBM3". The Human Protein Atlas. "Expression of RBM3 in cancer - Summary". The Human Protein Atlas. Danno S, ... Based on patient survival data, high levels of RBM3 protein in tumor cells is a favourable prognostic biomarker in colorectal ... Journal of Cell Science. 117 (Pt 9): 1785-94. doi:10.1242/jcs.01026. PMID 15075239. Brill LM, Salomon AR, Ficarro SB, Mukherji ...

*GIT2

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Ku GM, Yablonski D, Manser E, Lim L, Weiss A (Feb 2001). "A PAK1-PIX-PKL complex is activated by the T-cell receptor ... The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA ...

*ARPP-21

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ...

*USP6NL

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. Lanzetti L, Margaria V, Melander F, Virgili L, Lee MH, ... deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Research. 1 (1): 27-35. ... "Regulation of the Rab5 GTPase-activating protein RN-tre by the dual specificity phosphatase Cdc14A in human cells". The Journal ...

*EPS15L1

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell Biol. 153 (5): 1111-20. doi:10.1083/jcb.153.5.1111. PMC 2174325 . PMID 11381094. Poupon V, Polo S, Vecchi M, Martin G, ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. ... Cell Biol. 147 (7): 1379-84. doi:10.1083/jcb.147.7.1379. PMC 2174238 . PMID 10613896. Offenhäuser N, Santolini E, Simeone A, Di ...

*Drebrin-like

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... Cell. Biol. 19 (2): 1539-46. PMC 116082 . PMID 9891087. Ensenat D, Yao Z, Wang XS, Kori R, Zhou G, Lee SC, Tan TH (1999). "A ... Han J, Shui JW, Zhang X, Zheng B, Han S, Tan TH (2005). "HIP-55 is important for T-cell proliferation, cytokine production, and ... Cell. Biol. 25 (16): 6869-78. doi:10.1128/MCB.25.16.6869-6878.2005. PMC 1190228 . PMID 16055701. Tao WA, Wollscheid B, O'Brien ...

*PARD3

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ... "Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing ... "Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing ... "The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42". Nat Cell Biol. 2 (8): 531-9. doi:10.1038/ ...

*Phage display

By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein ... The use of a helper phage can be eliminated by using 'bacterial packaging cell line' technology. Elution can be done combining ... Then the expression of single chain Fv's (scFv), and single chain T cell receptors (scTCR) were expressed both with and without ... Endemann H, Model P (July 1995). "Location of filamentous phage minor coat proteins in phage and in infected cells". J. Mol. ...
O:13:PanistOpenUrl:36:{s:10:\u0000*\u0000openUrl;N;s:6:\u0000*\u0000idc;N;s:6:\u0000*\u0000fmt;s:7:journal;s:6:\u0000*\u0000doi;s:0:;s:6:\u0000*\u0000pii;s:0:;s:7:\u0000*\u0000pmid;s:0:;s:9:\u0000*\u0000atitle;s:73:A NOVEL APPROACH TO CELL IMMOBILIZATION WITH FILAMENTOUS ESCHERICHIA COLI;s:9:\u0000*\u0000jtitle;s:0:;s:9:\u0000*\u0000stitle;s:0:;s:7:\u0000*\u0000date;s:4:1980;s:9:\u0000*\u0000volume;s:0:;s:8:\u0000*\u0000issue;s:0:;s:8:\u0000*\u0000spage;s:0:;s:8:\u0000*\u0000epage;s:0:;s:8:\u0000*\u0000pages;s:0:;s:7:\u0000*\u0000issn;s:0:;s:8:\u0000*\u0000eissn;s:0:;s:9:\u0000*\u0000aulast;s:3:TSO;s:10:\u0000*\u0000aufirst;s:2:WW;s:9:\u0000*\u0000auinit;N;s:10:\u0000*\u0000auinitm;N;s:5:\u0000*\u0000au;a:1:{i:0;s:6:TSO ...
Whole cell immobilization of thermostable ?-amylase from phosphate solubilizing Bacillus thuringiensis A5-BRSC-IJMR-Print ISSN No:-2394-546X Online ISSN No:-2394-5478Article DOI No:-10.18231/2394-5478.2018.0055,Indian Journal of Microbiology Research-IP Innovative Publication Pvt Limited, Medical Journals Publication
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A biosensor is proposed that contains bacteria that naturally effluxes potassium ions when threatened by electrophilic species. Pseudomonas aeruginosa is an activated sludge isolate and possesses the characteristic potassium efflux response. It has been immobilized in calcium alginate beads, photopolymer disks, and a thermally reversible gel in order to ultimately incorporate the immobilized system into a functional biosensor. The potassium efflux and cell viability were measured in the immobilized matrices. Wastewater treatment is of utmost importance; however, processes are easily upset. Upsets can be caused by various electrophiles found in the environment, and can cause serious health effects to people or the environment downstream from an upset. Electrophiles can cause the activated sludge in wastewater treatment facilities to deflocculate, and untreated water can be lost downstream. Devising a detection system for proactively sensing electrophiles prior to an upset is an important ...
This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed …
Figure 1:Fluorescence images of PC3-GFP cells immobilized on ECM micro patterns of various shapes (the patterns are depicted by dashed lines).. Mastering the adhesion landscape of living cells on a surface is emerging as a new tool for investigating a lot of fundamental mechanisms of cell biology such as shape control, differentiation, division, polarity or motility. The common point of all these studies is the production of micro-patterns of various shapes and dimensions along which, adherent cells are immobilized in a deterministic way. The control of cell adhesion through technology allows the biologists to design specific scenarii and record how various living cells respond (neural, epithelial, tumoral and stem cells). Because technology allows reproducing identical precise patterns along well arranged periodical arrays, these experimental observations can be made systematically over large population of cells thus reaching a high level of representativeness. The micro-contact printing of ...
Selim, N., Hamed, H., Fathy, R., Gamal, M., El Kareem, H.A., Breheny, M., Farahmand, N., Bowman, K., Kyazze, G., Keshavarz, T. and Gomaa, O.M. 2018. Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation, salinity and endogenous biosurfactant induction. 4th European Meeting of the International Society for Microbial Electrochemistry and Technology. Newcastle University, Newcastle upon Tyne 12 - 14 Sep 2018 Design of a single chamber air cathode microbial fuel cell using a stainless steel spiral anode and 3D printing techniques for continuous flow dye decolourisation ...
Cells of a commercial Saccharomyces cerevisiae strain (Uvaferme 299) were immobilized on dried raisin berries (Sultanina variety) to produce an immobilized biocatalyst for use in dry white wine-making. The immobilised biocatalyst was found to be suitable for wine making at ambient temperatures (15-25 °C). The wines produced had low volatile acidities and low methanol and acetaldehyde contents, while volatile by-products showed no statistically significant differences from wines produced by free cells. The immobilized cell system had a good operational stability for more than 4 months. Sensory evaluation revealed differences between wines produced by immobilized and free cells ...
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Earlier this week, I published a brief article in this newsletter (at http://goo.gl/8oF3Nn) about a new cell phone service from Google. I mentioned that I thought it was overpriced at $20 to $30 a month or more. For more than two years, I have been using a similar service from a small-time cellular service provider…
Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in the inner part of a gel, gel fragility, cell leakage from the support matrix, and adverse effects on cell viability and catalytic activity. We previously showed a new method for bacterial cell immobilization using AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5. This approach is expected to solve the drawbacks of conventional immobilization methods. However, similar to all other immobilization methods, the use of support materials increases the cost of bioprocesses and subsequent waste materials. We found that the stickiness of the AtaA molecule isolated from Tol 5 cells is
Fermented rice noodle is a major source of food industry generating highly complex organic content (starch) wastewater. This study investigated the treatment of fermented rice noodle wastewater using calcium alginate entrapped yeast cells compared to the free cells. The treatment includes a two-step process: acid hydrolysis for breaking down starch to glucose and fermentation for degrading glucose to ethanol. Yeast culture, Saccharomyces cerevisiae, was used in this study. The experiment was conducted to examine optimum acid concentration and cell entrapment condition for fermentation. Sulfuric acid concentrations ranged from 0.25 to 1.00% by volume were tested while the cells entrapped in calcium alginate at cell-to-matrix (alginate) ratios (by volume) of 1:5, 1:10, and 1:20 were varied. The result showed that the optimum acid concentration of 1.00% provided 5-time higher glucose concentration compared to that in raw wastewater. After the batch fermentation, the entrapped cells reduced total ...
The performance of a novel reverse membrane bioreactor (RMBR) with encased microorganisms for syngas bio-methanation as well as a co-digestion process of syngas and organic substances was examined. The sachets were placed in the reactors and examined in repeated batch mode. Different temperatures and short retention time were studied. The digesting sludge encased in the PVDF membranes was able to convert syngas into methane at a retention time of 1 day and displayed a similar performance as the free cells in batch fermentation. The co-digestion of syngas and organic substances by the RMBR (the encased cells) showed a good performance without any observed negative effects. At thermophilic conditions, there was a higher conversion of pure syngas and co-digestion using the encased cells compared to at mesophilic conditions.[on SciFinder (R)]. ...
IMMOBILIZED CELLS + CELL IMMOBILIZATION (CYTOLOGICAL TECHNIQUES); MICROINJECTION (CYTOLOGICAL TECHNIQUES); ISOLATED CELLS (CYTOLOGICAL METHODS); ISOLIERTE ZELLEN (CYTOLOGISCHE METHODEN); MIKROINJEKTION (CYTOLOGISCHE METHODEN); IMMOBILISIERTE ZELLEN + IMMOBILISIERUNG VON ZELLEN (CYTOLOGISCHE METHODEN ...
Water is one of Ohios most important natural resources, and the State has an adequate supply to meet its immediate needs. Most of Ohios water problems are associated with water quality. Of primary concern are the sediments, nutrients and acids in the surface waters from urban, agricultural and mining areas, and the toxic and hazardous wastes that threaten the ground and surface waters. The focus of the 1988 State Water Research Program was directed at some of these needs. One project explored the design criteria for an innovative two-stage fluidized bed bioreator in which the three major processes of cell immobilization, biodegradation, and biofilm control were combined in a single unit. This wastewater treatment process is felt to be a substantial evolution in the operational technology of bioreactor design and the successful completion of this project could result in the development of an innovative, reliable and considerably less costly wastewater treatment system. Another projects ...
The ectoderm of the Xenopus embryo is permeated by a network of channels that appear in histological sections as interstitial gaps. We characterized this interstitial space by measuring gap sizes, angles formed between adjacent cells, and curvatures of cell surfaces at gaps. From these parameters, and from surface-tension values measured previously, we estimated the values of critical mechanical variables that determine gap sizes and shapes in the ectoderm, using a general model of interstitial gap mechanics. We concluded that gaps of 1-4 μm side length can be formed by the insertion of extracellular matrix fluid at three-cell junctions such that cell adhesion is locally disrupted and a tension difference between cell-cell contacts and the free cell surface at gaps of 0.003 mJ/m(2) is generated. Furthermore, a cell hydrostatic pressure of 16.8 ± 1.7 Pa and an interstitial pressure of 3.9 ± 3.6 Pa, relative to the central blastocoel cavity of the embryo, was found to be consistent with the ...
A soluble allogeneic effect factor (AEF) was produced by using H-2 congenic mouse strains and a serum.free cell culture medium. An AEF derived from untreated activated responder cells and irradiated stimulator cells provided helper cell function in a primary and secondary antibody response for both T-cell-depleted responder B cells and stimulator B cells. This interaction may be determined by genes situated in the I-A and I-B regions: additional K-region control was not excluded. Ia antigens, but neither H-2 nor Ig determinants are molecular constituents of AEF. The active components of this AEF consist, in part, of Ia antigens derived from both the activated responder cell population and irradiated stimulator cell population. An AEF derived from Ia negative responder cells and irradiated T-cell- depleted stimulator cells helps a secondary antibody response of T-cell- depleted stimulator B cells but not responder B cells. This genetically restricted AEF contains Ia antigens determined by the ...
Cell junctions are stably maintained in mature epithelial cells. When DAAM1 was depleted in EpH4 cells, however, the cell membranes forming LCs became mobile, similar to the leading edges of migrating cells. This enhancement of motility depended on the Rac-WRC signaling system, which is generally important for the formation of leading edges or lamellipodia in migrating cells (Krause and Gautreau, 2014). These results suggest that the membranes of LCs are motile by nature, like free cell edges, but this ability is normally restrained by DAAM1 (Fig. S5 F). Consistent with our observations, a previous study using DAAM1 gene trap mice showed that, in cardiomyocytes with a marked reduction of DAAM1, the distributions of F-actin, N-cadherin, and α-catenin were perturbed, leading to abnormal sarcomere organization and cell misalignment (Li et al., 2011).. DAAM1, as a member of the formin family, is thought to nucleate and/or accelerate the elongation of actin filaments. DAAM1 depletion induced ...
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Save 42% Jarrow Formulas - Ubiquinol QH-absorb 100 mg 60 Softgels Ubiquinol QH-absorb 100 mg Reduced Form Co-Q10 High Absorption & Enhanced Stability Q+ Kaneka - Made with Quality Ubiquinol Gluten Free Cells produce Ubiquinol, the active antioxidant state of CoQ10, which is significantly better absorbed, particularly as we age.* QH-absorb® is a proprietary enhanced-stability formula, clinicallly shown in humans to increase Co-Q10 levels by 215% at 100 mg per day and by 777% over baseline at 300 mg per day.
What we really come down to is our views on government spending and taxation. Is the money being spent on food stamps our money? Or is it the governments? Most of us here likely believe that it is our money being taken from us (by force) by the government and spent to take care of those who cant, or very often, wont take care of themselves. Few begrudge those who cant work through no fault of their own. The problem is the vast number who could, but wont. Esp., yes, the single mothers who start having kids without a husband, often with multiple males, for the benefits. It doesnt help that the takers were mobilized by the Dems so effectively to win this last election by promises of even more largess at the expense of the makers, those who do work hard for their money, taken to support the takers, the leech class. The fact that so few were troubled by ObamaPhones, where the Obama people took credit for giving free cell phones to so many of these people is a bit scary, paid for by, of course, ...
What we really come down to is our views on government spending and taxation. Is the money being spent on food stamps our money? Or is it the governments? Most of us here likely believe that it is our money being taken from us (by force) by the government and spent to take care of those who cant, or very often, wont take care of themselves. Few begrudge those who cant work through no fault of their own. The problem is the vast number who could, but wont. Esp., yes, the single mothers who start having kids without a husband, often with multiple males, for the benefits. It doesnt help that the takers were mobilized by the Dems so effectively to win this last election by promises of even more largess at the expense of the makers, those who do work hard for their money, taken to support the takers, the leech class. The fact that so few were troubled by ObamaPhones, where the Obama people took credit for giving free cell phones to so many of these people is a bit scary, paid for by, of course, ...
Big Pharmas goal is out to get every person on the planet on at least two prescription drugs. Hows that. And with all the many debilitating side effects of practically all Big Pharma drugs requiring more Big Pharma drugs for treatment, it appears as though the Drug Trust are well on their way, doesnt it? After all, the head honchos at the CDC all work in the revolving door system, moving from Big Pharma companies back and forth into the CDC and FDA to keep the fraud going. This is big business that has very little interest in keeping Americans safe and healthy, but who in reality are most interested in getting their poisons sold.. Now with Obamacare, (the intentionally socialist unaffordable Affordable Care Act) not only can poor folk get a free cell phone, there is easier access to get vaccines, for all, for FREE. This is also being advertised right outside your local Walgreens. ...
We have an entitlement system that keeps people addicted to the government dole," he said. "Its a culture of dependency that is funded, propagated and advertised by the federal government." Dasher uses the federal governments marketing of free cell phones to those who qualify as an example.. And he said the "culture of dependency" contributes to Americas illegal immigration issue.. "The demand for illegal labor has been created because we subsidize a large part of the (citizen) population not to work," he said.. As for what to do with those illegal immigrants already here, including unaccompanied children, Dasher said, "We should actively encourage the churches to get involved, but we dont as a country have the capacity to house and clothe millions of illegal immigrants.". Dasher has leveraged his link to the "Duck Dynasty" Robertson family - patriarch Phil Robertson is his uncle - into national conservative TV and radio talk show appearances and as a fundraising tool.. "Theyre very ...
Bottcher, I; Hammerling, G; and Kapp, J, "Continuous production of monoclonal mouse ige antibodies with known allergenic spec- ificity by a hybrid cell line." (1978). Subject Strain Bibliography 1978. 865 ...
The fact that JGL made a great leap in modernising production processes by introducing continuous production at its new location Svilno 2, thus recognising…
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Biocatalytic asymmetric reductions with whole cells can offer high enantioselectivity, environmentally benign processes and energy-effective operations and thus are of great interest. The application of whole cell-mediated bioreduction is often restricted if substrate and product have low water solubility and/or high toxicity to the biocatalyst. Many studies have shown that a biphasic system is often useful in this instance. Hence, we developed efficient biphasic reaction systems with biocompatible water-immiscible ionic liquids (ILs), to improve the biocatalytic anti-Prelog enantioselective reduction of acetyltrimethylsilane (ATMS) to (R)-1-trimethylsilylethanol {(R)-1-TMSE}, which is key synthon for a large number of silicon-containing drugs, using immobilized Candida parapsilosis CCTCC M203011 cells as the biocatalyst. It was found that the substrate ATMS and the product 1-TMSE exerted pronounced toxicity to immobilized Candida parapsilosis CCTCC M203011 cells. The biocompatible water-immiscible ILs
BIOLOGISCHE REAKTOREN (BIOTECHNOLOGIE); CYTOLOGISCHE BIOTECHNOLOGIE; IMMOBILISIERTE ZELLEN + IMMOBILISIERUNG VON ZELLEN (CYTOLOGISCHE METHODEN); SEKUNDÄRMETABOLITEN (PFLANZENCHEMIE); MEMBRANREAKTOREN (CHEMISCHE VERFAHRENSTECHNIK); BIOLOGICAL REACTORS (BIOTECHNOLOGY); CYTOLOGICAL BIOTECHNOLOGY; IMMOBILIZED CELLS + CELL IMMOBILIZATION (CYTOLOGICAL TECHNIQUES); SECONDARY METABOLITES (PHYTOCHEMISTRY); MEMBRANE REACTORS (CHEMICAL ENGINEERING ...
A method of recycling a support material for supporting images thereon, at least part of the support material including a paper layer which contains cellulose fibers as the main component and bears th
A support material for solid phase synthesis is provided having an amine-containing organic group attached to it through a linker. The support material is of the following general formula (Formula I): ##STR1##
Described is a photographic support material for black-and-white and color photographic emulsions, which support material is coated at least on one side with a layer containing a white pigment ad a bi
The internal structure of aerobic granules has a significant impact on the hydrodynamic performance and mass transfer process, and severely affects the efficiency and stability of granules-based reactors for wastewater treatment. In this study, for the first time the granule complex structure was correlated with the hydrodynamic performance and substrates reactions process. First, a series of multiple fluorescence stained confocal laser scanning microscopy images of aerobic granules were obtained. Then, the form and structure of the entire granule was reconstructed. A three-dimensional computational fluid dynamics study was carried out for the hydrodynamic analysis. Two different models were developed on the basis of different fluorescence stained confocal laser scanning microscopy images to elucidate the roles of the granule structure in the hydrodynamic and mass transfer processes of aerobic granules. The fluid flow behavior, such as the velocity profiles, the pathlines and hence the ...
The biotransformation of water insoluble substrates by mammalian and bacterial cells has been problematic, since these whole cell reactions are primarily performed in an aqueous environment The implementation of a twophase or encapsulated system has the advantages of providing a low water system along with the physiological environment the cells require to sustain themselves. Encapsulation of mammalian cells by formation of polyamide capsules via interfacial polymerization illustrated that the cells could not survive this type of encapsulation process. Biotransformation of the steroid spironolactone [3] by human kidney carcinoma cells was performed in a substrate-encapsulated system, yielding canrenone [4] in 70% yield. Encapsulation of nitrile-metabolizing Rhodococcus rhodochrous cells using a polyamide membrane yielded leaky capsules, but biotransformation of 2-(4- chlorophenyl)-3-methylbutyronitrile (CPIN) [6] in a free cell system yielded CPIN amide [7] in 40% yield and 94% ee. A two-phase ...
Stirred-tank bioreactors are gaining more and more importance in the industrial production of pharmaceutical products, biofuels and food. When compared with 2D-cultivation systems, bioreactors are easy to handle, and multiple bioreactors can be controlled in parallel without a considerable increase of workload. In stirred-tank bioreactors cells can be cultivated as free cells in solution, cell aggregates or on microcarriers in batch, fed-batch or continuous cultivation modes ...
E-cadherin interacts with other E-cadherin molecules on neighboring cells to form cell-cell adhesions. E-cadherin that is not involved in these trans interactions is removed from the cell surface and replaced with newly synthesized molecules to maintain dynamic adhesions. Now, Izumi et al. (page 237) show that E-cadherins that are involved in trans interactions are excused from this endocytosis by small GTPases. Disruption of this system may free cells for migration.. By reconstituting endocytosis in membrane bilayers, the group shows that clathrin-dependent endocytosis removes E-cadherin that is not interacting in trans with other E-cadherins. E-cadherins engaged in trans interactions, however, activated Rac and Cdc42, which blocked their internalization. So far it is unclear how the trans interactions activate the G proteins.. The endocytic block is enhanced by IQGAP1, an effector of Rac and Cdc42. IQGAP1 cross-links actin filaments into bundles, and the group shows that F-actin is needed to ...
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By Greg Mills. As I write this Sunday morning early, all the Apple news sites and a lot of regular news sites are running leaked information about the iPhone launch, scheduled for Tuesday. The normally tight new product security has been breeched by even Apple in a couple of ways. What seems clear is that Apple is moving to capture greater market share with more cellular carriers to be offering iPhones and with both a lower end and higher end offering. The iPhone 4S and iPhone 5 appear to be the two new iPhone models to be announced Tuesday.. There are two main markets the cellular carriers all support. They call the two plans "post-paid" and "pre-paid." Generally, the post-paid segment is the cream of the crop and the real money for the networks. This is the plan with a contract, generally for two years, where you get a red hot deal on a phone, or a free cell phone in exchange for signing up. With iPhones, you just pay a portion of the cost of the.... , Read more » ...
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So, not only are taxpayers funding free cell phones, were also paying for street teams to hawk them at hospitals, soup kitchens, carnivals and shelters. Apparently a cell phone is now a basic essential, like food, water and shelter, that is so necessary for a persons survival it must be provided for those who cant afford it. Whats next? An Obama car? An Obama computer? An Obama iPad? An Obama Espresso machine? I better stop. I dont want to give them any ideas ...
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Things to See and Do Downtown and the Waterfront A Contemporary Theatre (700 Union St.) Located just around the corner from the Convention Center, this regional theatre has showcased avant garde productions since 1965. Seattle Art Museum (1300 First Ave.) Even if you dont check out the exhibits in the recently expanded museum, you might want to consider checking out the fantastic cafe and gift shop. Seattle Public Librarys Central Library (1000 Fourth Ave.) Loved by many, scoffed at by some - if you havent seen it, you really should. Look for the Free Cell Phone Tour signs inside the building for a self-guided tour. Pike Place Market (First Ave. and Pike St.) More than just flying fish and crowds at Starbucks, the market is a vibrant hive of activity. Check out the many local merchants, including the anarchist bookstore Left Bank Books and the Anglophile Crumpet Shop. Top your shopping off with a dozen mini donuts made right in front of you at the Daily Dozen Doughnut Company. Seattle Great ...
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This invention relates to a process and a laminator for the continuous production of laminates of foam with facings. An upper facing and a lower facing and a web of meshwork are introduced into the foaming space of a laminator and a fluid reaction mixture is spread over the lower facing before it runs into the foaming space. The reaction mixture penetrates the web of meshwork as it foams up on its journey through the foaming space and becomes bonded to the facings.
article{960e9b68-d84e-438d-87fb-ee35adc2a226, abstract = {The complexes formed by the reaction of [Ti(OiPr)(4)] and bicyclo-octanediols (BODOLs) 1 and 2 (1:1) are useful as chiral catalysts in asymmetric reductions and were investigated by H-1 NMR spectroscopy and computational methods. A consistent picture emerged of head-to-tail dimers being kept together via a Ti-O-Ti-O mu-oxo bridge similar to the Ti-tartrates but different from the corresponding Ti-BINOLates and Ti-TADDOLates.}, author = {Sarvary, Ian and Norrby, P-O and Frejd, Torbjörn}, issn = {1521-3765}, language = {eng}, number = {1}, pages = {182--189}, publisher = {John Wiley & Sons}, series = {Chemistry: A European Journal}, title = {Structure investigation of Ti-IV-BODOLates involved in the catalytic asymmetric reduction of ketones using catecholborane}, url = {http://dx.doi.org/10.1002/chem.200305202}, volume = {10}, year = {2004 ...
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A new nad-dependent alcohol-dehydrogenase with opposite facial selectivity useful for asymmetric reduction and cofactor regeneration ...
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Iron Fit Nutrition strives to produce distinctive, affordable, premium-quality products while making every effort to anticipate ever-changing customer needs and meet the demands of a dynamic market with innovative products, effective support materials, and unsurpassed service ...
Microinnova Engineering GmbH is a pioneer in the field of continuous manufacturing. Our Process Development and Engineering departments in a combined effort provide process development, engineering services, scale-up and delivery of skid-based turnkey plants for continuous production ranging from kilo-lab to pilot- and industrial quantities. We are known as batch-to-conti experts applying the appropriate technology for each unique process challenge ...
In the early 1860s three Swedes, Nils Wilhelm Mitander,Julius Ramsay and Gustaf Wittenström, were engaged by theBritish to build and run charcoal-based ironworks in India.These works, the Burwai Iron Works of the British Government inthe case of Mitander and the privately owned Kumaon Iron Worksin the case of Ramsay and Wittenström, were both to bebased on the most modern European technology. The projects werepioneering in Indian ironmaking. The ambitions were high andstakes big, but after only a few years the projects were closedand the Swedes returned home.Landscapes of Technology Transferpresents a detailedstudy of the Kumaon and Burwai Iron Works, from their firstconception to their final closure. The investigation isbasically empirical and a fundamental question is: Why were theworks never brought into full and continuous production?. The ironworks projects should be considered as processes oftechnology transfer rather than fully fledged and completedtransfers. In spite of this lack of ...
Biological degradation of phenol by Alcaligenes faecalis with high biodegradation activity and high tolerance was investigated at 25C. Phenol could be utilized by the bacteria as the sole carbon and energy sources. The cell growth and substrate degradation of phenol as single substrates for Alcaligenes faecalis in batch cultures (shaking flasks) were investigated at different initial phenol concentrations. Phenol was observed to be an inhibitory compound. Particularly, when free cells grew on a high concentration of phenol, substrate inhibition was observed and the higher the concentration of phenol, the longer was the lag period. The lag time and whole required time for phenol biodegradation was considerably decreased by immobilized cells due to improvement of cells resistance against high Phenol concentration by cell immobilization compared to free cells. Required time for completely degrade initial phenol of 700 and 1000 ppm by free and immobilized cells is 56, 101 and 35, 72 hours ...
What is enzyme immobilization? How whole cells can be immobilized on a support? What are the advantages and disadvantages of immobilization? What are the applications of enzyme immobilization? The Supports and matrix used in enzyme immobilization technique, Different Types or methods of enzyme immobilization: Adsorption, Covalent bonding, Entrapment, Co-polymerization/cross linking and Encapsulation. Advantages and disadvantages of whole cell immobilization, Methods of whole cell immobilization. Learn more: Lecture Note in Enzyme Immobilization. You can DOWNLOAD the PPT by clicking on the download link below the preview…. ...
Sweet sorghum stalks were used as a low cost carrier for immobilization of Saccharomyces cerevisiae NP 01 to produce ethanol from sweet sorghum juice. The effects on ethanol production of carrier size (6 × 6 × 6 to 20 × 20 × 20 mm3) and initial cell concentrations (5 × 107 to 2 × 108 cells mL−1) for cell immobilization were investigated. The ethanol production medium was the juice containing 230 g L−1 of total sugar without nutrient supplementation. The fermentations were carried out under static conditions in 500-mL air-locked Erlenmeyer flasks at 30 °C. The results showed that the optimum size of sorghum stalk pieces for repeated-batch ethanol production was 6 × 6 × 6 mm3, while the optimum initial cell concentration for the immobilization was 1.0 × 108 cells mL−1. The immobilized yeast under these conditions could be used for at least eight successive batches without any losses of ethanol production efficiencies. The average ethanol concentration, productivity and yield of the eight
Introduction. There are numerous biotechnological processes that make use of immobilisation techniques to manipulate cells. These techniques can be divided into four major categories based on the physical mechanism at play in bringing about the immobilisation of the cells. These categories are (1) attachment or adsorption on solid carrier surfaces, (2) entrapment within a porous matrix, (3) natural aggregation by flocculation, and (4) artificially induced cross-linking by agents and/ or cell containment behind barriers.1 Amongst the available yeast cell immobilisation techniques, the flocculation of microorganisms is very attractive, because of its simplicity and low cost. Flocculation does not involve any complex and costly mechanical devices or any supporting material in its operation, which represents a significant advantage over other immobilisation techniques.2. Flocculation is defined as the phenomenon of loose aggregation of free yeast cells as a result of random collisions by Brownian ...
Introduction. There are numerous biotechnological processes that make use of immobilisation techniques to manipulate cells. These techniques can be divided into four major categories based on the physical mechanism at play in bringing about the immobilisation of the cells. These categories are (1) attachment or adsorption on solid carrier surfaces, (2) entrapment within a porous matrix, (3) natural aggregation by flocculation, and (4) artificially induced cross-linking by agents and/ or cell containment behind barriers.1 Amongst the available yeast cell immobilisation techniques, the flocculation of microorganisms is very attractive, because of its simplicity and low cost. Flocculation does not involve any complex and costly mechanical devices or any supporting material in its operation, which represents a significant advantage over other immobilisation techniques.2. Flocculation is defined as the phenomenon of loose aggregation of free yeast cells as a result of random collisions by Brownian ...
Near field generated by plasmonic structures has recently been proposed to trap small objects. We report the first integration of plasmonic trapping with microfluidics for lab-on-a-chip applications. A three-layer plasmo-microfluidic chip is used to demonstrate the trapping of polystyrene spheres and yeast cells. This technique enables cell immobilization without the complex optics required for conventional optical tweezers. The benefits of such devices are optical simplicity, low power consumption and compactness; they have great potential for implementing novel functionalities for advanced manipulations and analytics in lab-on-a-chip applications.. ©2009 Optical Society of America. Full Article , PDF Article ...
TY - JOUR. T1 - Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation. AU - Kuang, Liangju. AU - Damayanti, Nur P.. AU - Jiang, Chunhui. AU - Fei, Xing. AU - Liu, Wenjie. AU - Narayanan, Naagarajan. AU - Irudayaraj, Joseph Maria Kumar. AU - Campanella, Osvaldo. AU - Deng, Meng. PY - 2019/2/5. Y1 - 2019/2/5. N2 - Cell encapsulation within 3D hydrogels is an attractive approach to develop effective cell-based therapies. However, little is known about how cells respond to the dynamic microenvironment resulting from hydrogel gelation-based cell encapsulation. Here, a tunable biomimetic hydrogel system that possesses alterable gelation kinetics and biologically relevant matrix stiffness is developed to study 3D dynamic cellular responses during encapsulation. Hydrogels are synthesized by crosslinking thiolated hyaluronic acid and thiolated chondroitin sulfate with poly(ethylene glycol) diacrylate under cell-compatible conditions. Hydrogel properties are ...
Guinea pigs injected with heterologous renal basement membranes (RBM) and complete Freunds adjuvant (FA) develop a new renal cortical tubular disease and form antibodies that react with renal tubular basement membrane (TBM) (1-3). We now document the characteristics and immunopathology of this renal tubular disease and the presence of anti-TBM antibodies in the serum and along the cortical TBM. Finally, we demonstrate a positive correlation between the incidence of severe tubular disease and large amounts of anti-cortical TBM autoantibodies.. Materials and Methods. RBM, rich in TBM, were prepared from frozen New Zealand White rabbit kidneys3 by a modification of Krakower and Greenspons method (4). Briefly, rabbit cortex was buttered through a 100-mesh metal screen. The screenings were washed by centrifugation in saline to remove free cells. By repeated shaking and washing of the residue, glomeruli settled faster than tubules. Supernatants rich in tubules were examined microscopically (4, ...
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The aim of this thesis is to find catalysts which show high activity and selectivity for lean NOx reduction in the low-temperature regime relevant for diesel and lean-burn engines and to investigate methanol as reducing agent. The lean NOx reduction over supported silver catalysts is studied, focusing on the influence of the support material, silver loading, addition of hydrogen and the C/N molar ratio. Alumina based (sol-gel) and ZSM-5 based (ion-exchange) silver catalysts were prepared, characterized and evaluated in flow reactor experiments. Comparisons of Ag-Al2O3 (2 wt% Ag) and Ag-ZSM-5 (5 wt% Ag) during methanol- SCR conditions show higher NOx reduction over Ag-Al2O3. In studies of the influence of the silver loading in Ag-Al2O3, increasing the silver loading (0 - 4 wt%) extends or shifts the active temperature window towards lower temperatures, at the same time as the NOx reduction in the high-temperature region decreases. Ag-Al2O3 with 3 wt% Ag shows the most promising results. It is active for
Low health literacy is a massive problem in the UK, Europe and United States. The medical community in general has an ongoing problem with low patient adherence to treatments, and this is one problem that could be effectively tackled with better patient support materials. Better in this case means support materials that patients with lower health literacy can understand more readily.
Creating support materials that give plenty of information as well as comprehensive instructions or advice on living with a condition can be a difficult undertaking. Medical writers natural language will include terms outside general education levels, and a fine line exists between simplification and dumbing down. | Social Marketing - Newsletter 151
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A cell system was developed for the reproducible detection of human T-lymphotropic retroviruses (HTLV family) from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS). The cells are specific clones from a permissive human neoplastic T-cell line. Some of the clones permanently grow and continuously produce large amounts of virus after infection with cytopathic (HTLV-III) variants of these viruses. One cytopathic effect of HTLV-III in this system is the arrangement of multiple nuclei in a characteristic ring formation in giant cells of the infected T-cell population. These structures can be used as an indicator to detect HTLV-III in clinical specimens. This system opens the way to the routine detection of HTLV-III and related cytopathic variants of HTLV in patients with AIDS or pre-AIDS and in healthy carriers, and it provides large amounts of virus for detailed molecular and immunological analyses. ...
Continuous Processing Continuous production is a flow production method used to manufacture, produce, or process materials without interruption. Continuous production is called a continuous process or a continuous flow process because the materials, either dry bulk or fluids that are being processed are continuously in motion, undergoing chemical reactions or subject to mechanical or heat…
The survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. As an alternative strategy, bioaugmentation of a group of...
You are viewing an interactive 3D depiction of the molecule n,n,n-trimethyl-4-(2-oxo-1-pyrrolidinyl)-2-butyn-1-aminium (C11H20N2O) from the PQR.
The adsorption of CO2 and CO was investigated on a pure γ-Al2O3 support material that has been used in Pd and Ru catalysts for the reduction of CO2. The adsorption of CO2 resulted in the formation of carbonates, bicarbonates and linearly adsorbed CO2 species. The amount and the nature of the adsorbed species
A system responsive to coordinate information for automatically providing a three-dimensional physical model of a desired geometry and comprising apparatus for selectably solidifying a solidifiable material on a sequential layer by layer basis characterized in that following selectable solidification of a given layer, the non-solidified portions thereof are removed and replaced by a removable support material which is not solidifiable under the same conditions as the solidifiable material.
This pack is the DNA and DNA ladder refill for the HNPCC: Detecting Inherited Forms of Cancer CarolinaBLU™ Kit (item #214820). Pack does not come with any additional instructions or support material. It is designed to be used in conjunction with the kit.
Data (Southern) Enterprises Ltd Registered Office: DS Medical, Eagle Building - E2, Daedalus Park, Daedalus Drive, Lee-on-the-Solent, PO13 9FX, ...
The increasing production of several plastics such as expanded polystyrene, widely used as packaging and building materials, has caused the release of considerable amounts of pentane employed as an expanding agent. Today many microorganisms are used to degrade hydrocarbons in order to minimize contamination caused by several industrial activities. The aim of our work was to identify a suitable microorganism to degrade pentane. We focused our attention on a strain of Arthrobacter sp. which in a shake-flask culture produced 95% degradation of a 10% mixture of pentane in a minimal medium after 42days of incubation at 20°C. Arthrobacter sp. cells were immobilized on a macroporous polystyrene particle matrix that provides a promising novel support for cell immobilization. The method involved culturing cells with the expanded polystyrene in shake-flasks, followed by in situ growth within the column. Scanning electron microscopy analysis showed extensive growth of Arthrobacter sp. on the polymeric ...
Effect of Natural and Pretreated Soybean Hulls on Enzyme Production by Trichoderma reesei. (A. M. Coffman, Q. Li, L.-K. Ju) Journal of the American Oil Chemists Society 91 (8), 1331-1338 (2014). View Article. Promoting Pellet Growth of Trichoderma reesei Rut C30 by Surfactants for Easy Separation and Enhanced Cellulase Production. (N.V. Callow and L.-K. Ju) Enzyme and Microbial Technology 50(6-7), 311-317 (2012). View Article. Cellulase production by continuous culture of Trichoderma reesei Rut C-30 using acid hydrolysate prepared to retain more oligosaccharides for induction. (C.-M. Lo, Q. Zhang, N. V. Callow and L.-K. Ju) Bioresource Technology. 101(2), 717-23 (2010). View Article. Cell immobilization with polyurethane foam for retaining Trichoderma reesei cells during foam fractionation for cellulase collection. (Q. Zhang, C.-M. Lo, and L.-K. Ju) Applied Biochemistry and Biotechnology 156, 12-23 (2009). View Article. Cellulase production by cocultures of Hypocrea jecorina Rut C30 and Candida ...
Puritan Calgiswab Sterile Calcium Alginate Mini-Tip (Nasopharyngeal) Swabs with Flexible Aluminum Shaft offer better culture isolation from areas such as the eyes, ears, nasopharynx, urethra and pediatric ENT Nasopharyngeal Tip Swabs. Non-irritating & non-sensitizing tips. Made in USA. Save at Harmony.
Bulky substituents in the endo-3-position of tropanes ( 1 ) can lead to adoption of the boat form (2) or to flattening15J of the chair. The endo-3-methyl compound ( 1 d) can be prepared by reaction of tropinone with methyllithium, dehydration of the resulting tertiary alcohol, and subsequent hydrogenation. Its existence in the boat form (2) can be ruled out in the same way as for tropine ( I b ) on the basis of the I3C-NMR shifts, which in particular for C3 should appear some -7 ppm upfield for (2). In fact the signals of C3 and (C3)-CH3 recorded above 230K display a broadening of 1-2 Hz which must be attributed to some slight participation of the boat form (2). The flattening of the piperidine chair reduces hindrance by the axial hydrogen atoms on C2 and C4 and thus shifts the N-invertomer equilibrium in favor of a-(I) (see Table 1). Preliminary measurements confirm the shift of the invertomer equilibrium by protic solvents which is frequently discussed for piperidine. It appears that the ...
An extrusion system that can create a polymer/gas solution rapidly for continuous processing of microcellular plastics is presented. Microcellular plastics are characterized by cell densities greater than 109 cells/cm3 and fully grown cells smaller than 10 μm. Previously these microcellular structures have been produced in a batch process by saturating a polymeric material with an inert gas under high pressure followed by inducing a rapid drop in the gas solubility. The diffusion phenomena encountered in this batch processing is typically slow, resulting in long cycle times. In order to produce microcellular plastics at industrial production rates, a means for the rapid solution formation is developed. The processing time required for completing the solution formation in the system was estimated from experimental data and the dispersive mixing theory based on an order-of-magnitude analysis. A means for promoting high bubble nucleation rates in the gas-saturated polymer via rapid heating is also ...
High molecular weight linear polycarbonates are prepared by continuous condensation of organic dihydroxy compounds and phosgene in two reaction zones, a mixture of an aqueous-alkaline solution of the organic dihydroxy compound and an aqueous catalyst solution being introduced at the rate of from 1 to 50 meters per second and the phosgene at the rate of from 30 to 300 meters per second into the first reaction zone and condensed, an organic solvent being incorporated into the resultant reaction mixture containing oligomer and polycarbonate and the condensation of the aqueous-organic reaction mixture carried to completion in the second reaction zone by the two-phase interfacial method.
Comprehensive profiles of industry players that are currently engaged in the preclinical / clinical development of their proprietary encapsulated cell therapies, featuring an overview of the company, its financial information (if available), and a detailed description of its product(s), highlighting mechanism of action, current development status, and key preclinical / clinical trial results. Each profile also includes a list of recent developments, highlighting the key milestones achieved, partnership activity, and the likely strategies that may be adopted by these players to fuel growth in the foreseeable future ...
Figure 1: (Top) Synthesis of SHINERS as a support material for catalysts, starting from bare gold nanoparticles, coated with an oxide layer and subsequently fixing catalyst particles on the surface. (Bottom) SHINERS used to study the Fischer-Tropsch process in realistic reaction conditions [3]. [1] T. Hartman, C. S. Wondergem, N. Kumar, A. Van Den Berg, B. M. Weckhuysen, J. Phys. Chem. Lett. 2016, 7, 1570-1584.. [2] J. F. Li, Y. F. Huang, Y. Ding, Z. L. Yang, S. B. Li, X. S. Zhou, F. R. Fan, W. Zhang, Z. Y. Zhou, D. Y. Wu, B. Ren, Z. L. Wang, Z. Q. Tian, Nature 2010, 464, 392-395.. [3] T. Hartman, B. M. Weckhuysen, Chem. Eur. J. 2018, 24, 3733-3741. ...
Description: Subject matter in which the assay system requires at least one separation step which allows differentiation of reacted from unreacted material or requires that at least one of the immunochemicals in the system be bound to an insoluble support material ...
Description: Subject matter in which the assay system requires at least one separation step which allows differentiation of reacted from unreacted material or requires that at least one of the immunochemicals in the system be bound to an insoluble support material ...
This Eagle Books Toolkit is designed to help you maximize the effectiveness of the Eagle Books series. By enlisting Eagle Books engaging activities and support materials for use in homes, classrooms, and communities, you help build upon the foundation of the series and sustain the valuable messages far beyond a simple reading of each story ...
As you learn more about breastfeeding, you may come across some unfamiliar terms. Here is a handy list of definitions for terms often found in breastfeeding support materials. This glossary of common breastfeeding terms is a great resource for when you come across unfamiliar terms while learning about feeding your bab
alcanoic acids (PHB) from substrates which are potentially ecotoxic. The inventive method for the continuous production of PHB is characterised in that strains of micro-organisms which are known to form PHB are bred chemostatically with maximum heat production on substrates which present growth inhibition with a substrate surplus. The advantages are, compared to other methods, that the invention represents a relatively cost-efficient way of microbial synthesis of polyhydroxyalcanoic acids. Waste products from chemical industries and agricultulture may be used for synthesis of PHB (utilizing phenol or benzoate as alternative carbon-sources). Thus, enabling simultaneous synthesis of useful materials as well as disposal of hazardous substances. The maximum heat produced is adjusted according to a maximum proportion of PHB contained in the biomass ...
Looking for online definition of calcium alginate in the Medical Dictionary? calcium alginate explanation free. What is calcium alginate? Meaning of calcium alginate medical term. What does calcium alginate mean?
The high cost of soluble enzymes can limit their use for commercial and industrial purposes. Immobilization can enhance enzyme reusability, thereby reducing product isolation costs and overcoming this economic barrier. In the current study, two novel, purified lipases from Pseudomonas sp. (Pseudomonas reinekei and Pseudomonas brenneri) were entrapped in a calcium alginate matrix, with the aim of simultaneously enhancing enzyme reusability and stability. Following entrapment, the retained activity of the enzyme-alginate composite was verified by an enzymatic hydrolysis reaction of a p-nitrophenol palmitate substrate. The effect of the enzyme-alginate entrapment against various physiochemical parameters such as pH, temperature, metal ions, and solvents were subsequently examined. The entrapment was found to have minimal beneficial stability gains. However, enhanced enzyme reusability (up to 3 cycles) and stability (up to 18 days at 4°C) of the calcium alginate entrapped lipase, as indicated by
China High Absorption Silver Antibacterial Alginate Dressing, Find details about China Antibacterial Alginate Dressing, Silver Calcium Alginate from High Absorption Silver Antibacterial Alginate Dressing - Huizhou Foryou Medical Devices Co., Ltd.
Fermented food, enjoyed across the globe, conveys health benefits through lactic acid fermentation. The fermentation process can transform the flavor of food from the plain and mundane to a mouth-puckering sourness enlivened by colonies of beneficial bacteria and enhanced micronutrients. While fermented food like yogurt, sauerkraut and kefir are well-known many other lesser-known foods also benefit from the lactic acid fermentation process. Indeed, virtually every food with a complex or simple sugar content can be successfully fermented.. Born of both necessity and practicality, lactic acid fermentation proved to be not only an efficient method of preserving food for our ancestors, but also a critical one. Indeed, fermented food like sauerkraut, cheese, wine, kvass, soured grain porridge and breads often sustained tribes and villages during harsh winters when fresh food simply wasnt available let alone plentiful.. In many societies including our own where yogurt has been heralded as a health ...
Propionic acid fermentation is carried out by several bacteria that belong to the genus Propionibacterium and to the species Clostridium propionicum. During propionic acid fermentation, both sugar and lactate can be used as the initial substrate. When sugar is available, these bacteria use the EMP pathway to produce pyruvate; the pyruvate is carboxylated to oxalacetate by methyl malonyl coen-zyme-A (CoA) and then reduced to propionate via malate, fumarate, and succinate. The other end products of propionic fermentation are acetic acid and CO2 (Figure 3). In particular, the propionic acid fermentation of 3 mol of glucose produces 4 mol of propionic acid, 2 mol of acetic acid, 2 mol of CO2, and 12 mol of ATP. When lactate is the initial substrate, propionic fermentation results in the production of 2 mol of propionic acid, 1 mol of acetic acid, and 1 mol of CO2. In this process, 1 mol of ATP is generated per nine carbons, and because of this, propionic bacteria generally grow very slowly.. The ...
Catalyst support materials exhibit great influence on the performance and durability of proton exchange membrane (PEM) fuel cells. This minireview article summarises recent developments into carbon nanotube-based support materials for PEM fuel cells, including the membrane electrode assembly (MEA). The advan

Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(iii) complexes - Chemical...Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(iii) complexes - Chemical...

Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes J. Cao, C. ... Targeting cancer cell metabolism with mitochondria-immobilized phosphorescent cyclometalated iridium(III) complexes ... Cancer cell metabolism is reprogrammed to sustain the high metabolic demands of cell proliferation. Recently, emerging studies ...
more infohttp://pubs.rsc.org/en/content/articlelanding/2016/sc/c6sc02901a

Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads.Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads.

The fermentation capacity of the immobilized yeast cells was found to be practically independent of the hydrogen ion ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. ... Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. The fermentation capacity of the ... The fermentation capacity was maximal at 30 degrees C. The immobilized yeast cells were used for continuous production of ...
more infohttp://www.biomedsearch.com/nih/Continuous-production-ethanol-using-yeast/8987641.html

Immobilized Cells: Basics and Applications, Volume 11 - 1st EditionImmobilized Cells: Basics and Applications, Volume 11 - 1st Edition

Purchase Immobilized Cells: Basics and Applications, Volume 11 - 1st Edition. Print Book & E-Book. ISBN 9780444819840, ... Immobilized-cell reactors. Scale up of immobilized-cell reactors (J.J. Heijnen). PEGASUS: innovative biological nitrogen ... Industrial applications of immobilized biocatalysts in Japan (T. Shibatani). Immobilized cell technology in food processing (C. ... Immobilized-cell growth: diffusion limitation in expanding micro-colonies (R.H. Wijffels et al.). Competition and cooperation ...
more infohttps://www.elsevier.com/books/immobilized-cells-basics-and-applications/buitelaar/978-0-444-81984-0

Patent US4600692 - Immobilized cells for preparing phenylalanine - Google PatentsPatent US4600692 - Immobilized cells for preparing phenylalanine - Google Patents

The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free ... The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also ... for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having ... as well as other forms of immobilized cells, provided the immobilized cells have transaminase activity. According to the ...
more infohttp://www.google.ca/patents/US4600692

Immobilized cells | definition of immobilized cells by Medical dictionaryImmobilized cells | definition of immobilized cells by Medical dictionary

... immobilized cells explanation free. What is immobilized cells? Meaning of immobilized cells medical term. What does immobilized ... Looking for online definition of immobilized cells in the Medical Dictionary? ... Immobilized yeast cells were found to be superior to the free yeast cells since immobilized cells are more tolerant to ethanol ... Alginate immobilized cells have been used in cell transplantation where it acts as the barrier between transplant (immobilized ...
more infohttps://medical-dictionary.thefreedictionary.com/immobilized+cells

Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier...Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier...

BRIN-BD11 cells immobilized on cytodex-1 or cultispher-Gout-performed single cell suspensions and pseudoislets in terms ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ... Similar growth profiles were recorded for eachmicrocarrier with densities of 4.4×105±0.3 cells/ml and4.2×105±0.2 cells/ml ... Culture and Function of Electrofusion-Derived Clonal Insulin-Secreting Cells Immobilized on Solid and Macroporous Microcarrier ...
more infohttps://portlandpress.com/bioscirep/article-abstract/20/3/167/54636/Culture-and-Function-of-Electrofusion-Derived?redirectedFrom=fulltext

Oenococcus oeni cells immobilized on delignified cellulosic material for malolactic fermentation of wine. - Semantic ScholarOenococcus oeni cells immobilized on delignified cellulosic material for malolactic fermentation of wine. - Semantic Scholar

... oeni cells immobilized on DCM took place at 27 degrees C. From the 3rd MLF batch up to 10th, the malic acid degradation was ... and the cfu of the immobilized cells/g of biocatalyst remained stable. The produced lactic acid was less than the ... batches of white must of 11-12 degrees Be initial density were performed by Saccharomyces cerevisiae cells immobilized on ... Oenococcus oeni ATCC 23279 cells immobilized on delignified cellulosic material (DCM) were used for malolactic fermentation ( ...
more infohttps://www.semanticscholar.org/paper/Oenococcus-oeni-cells-immobilized-on-delignified-of-Agouridis-Kopsahelis/1e3ce6d9d06d8c691247105f81692e8aa5b5478b

Immobilized Cell Technology - WikipediaImmobilized Cell Technology - Wikipedia

Immobilized cell technology is a method of air filtration and purification that uses whole cell immobilization. It is a process ... Oxidation through immobilized cell technology is 12 times more efficient than natural oxidation is under the same conditions. ... As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that ... The key difference between traditional air filtration systems and those that employ immobilized cell technology is that the ...
more infohttps://en.wikipedia.org/wiki/Immobilized_Cell_Technology

The spontaneous and induced synthesis of Epstein-Barr virus antigens in Raji cells immobilized on surface coated with anti...The spontaneous and induced synthesis of Epstein-Barr virus antigens in Raji cells immobilized on surface coated with anti...

... of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen ... of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen ... Immobilized Raji cells proved to be suitable targets for superinfection with EBV derived from P3HR1 cultures. ... The spontaneous and induced synthesis of Epstein-Barr virus antigens in Raji cells immobilized on surface coated with anti- ...
more infohttps://epub.uni-regensburg.de/20710/

Marine Drugs  | Free Full-Text | Biotransformation and Detoxification of Xylidine Orange Dye Using Immobilized Cells of Marine...Marine Drugs | Free Full-Text | Biotransformation and Detoxification of Xylidine Orange Dye Using Immobilized Cells of Marine...

Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-( ... the cells were immobilized in sodium alginate beads since immobilized cells had several advantages over suspended cells [16]. ... Such immobilized and stored cells can regain their viability upto several months (1-3 months) which can be an important ... The method of using L. sphaericus D3 cells immobilized in natural gel alginate beads was stable for longer periods, and with ...
more infohttps://www.mdpi.com/1660-3397/15/2/30/htm

Malic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe | American Journal of Enology and ViticultureMalic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe | American Journal of Enology and Viticulture

All experiments were carried out using dry immobilized yeast cells instead of wet immobilized yeast cells as in previous works ... the high malate fermentation activity of the immobilized yeast cells was similar to that of the non-immobilized cells. ... Alginate bead quality tests showed that immobilized cells could be recycled up to five times without liberation of yeast cells ... Malic Acid Consumption by Dry Immobilized Cells of Schizosaccharomyces pombe Sofía Silva, Felipe Ramón-Portugal, Patricia ...
more infohttp://www.ajevonline.org/content/54/1/50

Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells...Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells...

Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ... Fermentation of Groundnut Shell Enzymatic Hydrolysate for Fuel Ethanol Production by Free and Sorghum Stalks Immobilized Cells ...
more infohttps://www.degruyter.com/view/j/ijcre.2011.9.issue-1/1542-6580.2514/1542-6580.2514.xml

Nanoscopy of bacterial cells immobilized by holographic optical tweezersNanoscopy of bacterial cells immobilized by holographic optical tweezers

... Diekmann R, Wolfson D, Spahn C, Heilemann M, ... Diekmann R, Wolfson D, Spahn C, Heilemann M, Schüttpelz M, Huser T. Nanoscopy of bacterial cells immobilized by holographic ... "Nanoscopy of bacterial cells immobilized by holographic optical tweezers". Nature Communications 7 (2016): 13711. ... Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nature Communications, 7: 13711. ...
more infohttps://pub.uni-bielefeld.de/publication/2907008

Immobilized whole cell - WikipediaImmobilized whole cell - Wikipedia

... in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required ... The immobilized whole cell system is an alternative to enzyme immobilization. Unlike enzyme immobilization, where the enzyme is ... However, some enzymes may be used for the metabolic needs of the cell, leading to reduced yield of the cell. Zaushitsyna, O.; ... Also, if a series of enzymes are required in the reaction; whole cell immobilization may be used for convenience.This is only ...
more infohttps://en.wikipedia.org/wiki/Immobilized_whole_cell

High Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum | SpringerLinkHigh Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum | SpringerLink

The use of immobilized cells o. N. tabacum in sodium alginate gel much improved the yield of the products; the glycosylation of ... Glycosylation β-thujaplicin β-glucoside β-gentiobiosid Nicotiana tabacum immobilized cells This is a preview of subscription ... High Production of β-Thujaplicin Glycosides by Immobilized Plant Cells of Nicotiana Tabacum. ... were obtained through the biotransformation with immobilized cells in the medium without iron ions. In comparison with the case ...
more infohttps://link.springer.com/article/10.1556%2FABiol.59.2008.3.8

Immobilized-cell membrane bioreactor for high-strength phenol wastewater | ScholarBank@NUSImmobilized-cell membrane bioreactor for high-strength phenol wastewater | [email protected]

In the immobilized system, it was observed that cells diffused from the membranes when phenol concentrations reached ... An immobilized-cell membrane bioreactor was fabricated to investigate degradation of phenol at high concentrations using ... On the other hand, cells immobilized in 25% by weight polysulfone fibers degraded phenol at concentrations above 1,000 mg/L. At ... Loh, K.-C., Chung, T.-S., Ang, W.-F. (2000-01). Immobilized-cell membrane bioreactor for high-strength phenol wastewater. ...
more infohttps://scholarbank.nus.edu.sg/handle/10635/92043

Carrier-bound or immobilized enzyme or microbial cell; carrier-bound or immobilized cell; preparation thereof > Enzyme or...Carrier-bound or immobilized enzyme or microbial cell; carrier-bound or immobilized cell; preparation thereof > Enzyme or...

... carrier-bound or immobilized cell; preparation thereof > Enzyme or microbial cell is immobilized on or in an organic carrier > ... Method for detection of specific target cells in specialized or mixed cell population and solutions containing mixed cell ... Name: Chemistry: molecular biology and microbiology > Carrier-bound or immobilized enzyme or microbial cell; ... Electron mediator, electron mediator immobilized electrode, and biofuel cell using the electrode. Jun. 9, 2009. ...
more infohttp://www.patentgenius.com/class/435/181.html

Cell growth on immobilized cell-growth factor. 10. Insulin and polyallylamine co-immobilized materials. - Semantic ScholarCell growth on immobilized cell-growth factor. 10. Insulin and polyallylamine co-immobilized materials. - Semantic Scholar

The co-immobilized membrane can be applied for a new technique for protein-free cell culture. ... was co-immobilized with a cell growth factor, insulin, onto a surface-hydrolysed poly(methyl methacrylate) membrane. The ... The adhesion and growth of the cells were both markedly accelerated by the co-immobilization of the polyallylamine and insulin ... adhesion of mouse STO fibroblast cells was accelerated by the immobilization of polyallylamine. ...
more infohttps://www.semanticscholar.org/paper/Cell-growth-on-immobilized-cell-growth-factor.-10.-Zheng-Ito/446f5668cbe22828f62b9ba1d23725d3e879894e

Frontiers | Simultaneous Alcoholic and Malolactic Fermentations by Saccharomyces cerevisiae and Oenococcus oeni Cells Co...Frontiers | Simultaneous Alcoholic and Malolactic Fermentations by Saccharomyces cerevisiae and Oenococcus oeni Cells Co...

The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized cells could be efficiently reused for the wine fermentation at least three times without any apparent loss of ... The immobilized-cell system, that we here describe for the first time in our knowledge, offers many advantages over ... In recent years, the use of immobilized cell systems has been investigated, with interesting results, for the production of ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.00943/full

Effects of infrared and ultraviolet radiation on the viability of cells immobilized in porous TiNi-bEffects of infrared and ultraviolet radiation on the viability of cells immobilized in porous TiNi-b

IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, ... Effects Of Infrared And Ultraviolet Radiation On The Viability Of Cells Immobilized In Porous TiNi-based Alloy Scaffold ... In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell ... Cell cultures, cell tissue engineering, porous TiNi scaffold, IR and UV radiation. ...
more infohttps://www.vbripress.com/aml/articles/details/706

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells | Protocol (Translated to Swedish)A Rapid Technique for the Visualization of Live Immobilized Yeast Cells | Protocol (Translated to Swedish)

Zawadzki, K., Broach, J. A Rapid Technique for the Visualization of Live Immobilized Yeast Cells. J. Vis. Exp. (1), e84, doi: ... Cell 2006.. Protocol. * Immobiliserade celler för tidsförloppet epifluorescence mikroskopi. Observera att detta protokoll är ... Min mikroskopi visade att det inte finns något samband mellan den epigenetiska tillstånd HML och HMR i en enskild cell. sir1 ... Detta är en snabb teknik som immobilizes jästceller samtidigt tillåta tillväxt. Vi har följt jäst tillväxt för upp till tio ...
more infohttps://www.jove.com/video/84/en-snabb-teknik-fr-visualisering-av-live-immobiliserade-jstceller?language=Swedish

NDL India: Microbial fermentations with immobilized cellsNDL India: Microbial fermentations with immobilized cells

Ministry of Human Resource Development (MHRD) under its National Mission on Education through Information and Communication Technology (NMEICT) has initiated the National Digital Library of India (NDL India) pilot project to develop a framework of virtual repository of learning resources with a single-window search facility. Filtered and federated searching is employed to facilitate focused searching so that learners can find out the right resource with least effort and in minimum time. NDL India is designed to hold content of any language and provides interface support for leading Indian languages. It is being arranged to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular form of access devices and differently-abled learners. It is being developed to help students to prepare for entrance and competitive examination, to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to ...
more infohttps://ndl.iitkgp.ac.in/document/xolZJuS5Y-0gUz-mvR-omZWd5TEyr_QjUa-x7F6VupP9NSYjeiP-ZlhhBZ-CvtBI6sauYY2NOvdqF1qYiVESdA

A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells.A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells.

A rapid biosensor working with immobilized human U-937 cells was developed and applied to environmentally relevant chemicals ... Cells, Immobilized / drug effects*, pathology. Diclofenac / toxicity. Dose-Response Relationship, Drug. Environmental ... A rapid biosensor working with immobilized human U-937 cells was developed and applied to environmentally relevant chemicals ... Respiration of cells was relied upon as a comprehensive biochemical effect for screening purposes. Analytical parameter ( ...
more infohttp://www.biomedsearch.com/nih/new-respirometric-endpoint-based-biosensor/18499252.html

Immobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide Detection | Science...Immobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide Detection | Science...

This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and ... The immobilized cells were used as a bio-component for spectrophotometric detection of OP compounds, with MP as a ... Scanning electron microscope and cell count analyses revealed highly dense, rod shaped bacterial cells stably attached to the ... Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading ...
more infohttps://thescipub.com/abstract/10.3844/ojbsci.2018.332.340
  • The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. (degruyter.com)
  • Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles. (degruyter.com)
  • Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB , gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were immobilized onto the polystyrene surface of the wells of microtiter plates using adsorption method, followed by cross-linking with glutaraldehyde. (thescipub.com)
  • The method involved culturing cells with the expanded polystyrene in shake-flasks, followed by in situ growth within the column. (eugris.info)
  • In view of the advantages of the bulk production of clonal pancreaticbeta cells, an investigation was made of the growth and insulin secretoryfunctions of an electrofusion-derived cell line (BRIN-BD11) immobilizedon a solid microcarrier, cytodex-1 or a macroporous microcarrier,cultispher-G. For comparison, similar tests were performed usingBRIN-BD11 cells present in single cell suspensions or allowed toform pseudoislets. (portlandpress.com)
  • Insulin concentration in the culture medium reached a peak of26±2.0 ng/ml and 24±2.2 ng/ml for cells grown oncytodex-1 and cultispher-G, respectively. (portlandpress.com)
  • 10. Insulin and polyallylamine co-immobilized materials. (semanticscholar.org)
  • Subject matter wherein the enzyme or microbial cell is bonded to the polymeric carrier through an intermediate compound which attaches to both the enzyme and the carrier. (patentgenius.com)
  • 2, 3 and 500 sodium alginate concentrations were used for production and reusability of immobilized cells for 5 more trials. (thefreedictionary.com)
  • A rapid biosensor working with immobilized human U-937 cells was developed and applied to environmentally relevant chemicals with different structures and toxicological pathways, i.e. benzalkonium chloride, clofibric acid, diclofenac, mercury nitrate, ofloxacin, and sodium dodecyl sulphate. (biomedsearch.com)
  • In the immobilized system, it was observed that cells diffused from the membranes when phenol concentrations reached noninhibitory levels in a few experiments. (nus.edu.sg)
  • 17. The process of claim 16 wherein the immobilized cells are Rhodotorula glutinis cells and wherein the polyazetidine polymer is coated onto a particulate support. (google.ca)
  • On the other hand, 4-isopropyltropolone 2-O-β-D-glucoside (14%), 4-isopropyltropolone 2-O-β-D-gentiobioside (7%), 6-isopropyltropolone 2-O-β-D-glucoside (33%), and 6-isopropyltropolone 2-O-β-D-gentiobioside (13%) were obtained through the biotransformation with immobilized cells in the medium without iron ions. (springer.com)
  • A phosphorylcholine-modified chitosan polymer as an endothelial progenitor cell supporting matrix. (semanticscholar.org)
  • Is the Subject Area "Endothelial cells" applicable to this article? (plos.org)
  • As air moves through an immobilized cell technology system, those charged particles are attracted to a water cascade, that functions as neutral ground, and that pulls the particulates into the bioreactor. (wikipedia.org)
  • Alginate immobilized cells have been used in cell transplantation where it acts as the barrier between transplant ( immobilized cell ) and host immune system. (thefreedictionary.com)
  • This immobilized cell microtiter plate system has a great potential to be utilized as an alternative for OP detection and screening with the advantages of simplicity, stability, ease and convenience for assembly and use, and low cost. (thescipub.com)
  • At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. (uni-regensburg.de)
  • BRIN-BD11 cells immobilized on cytodex-1 or cultispher-Gout-performed single cell suspensions and pseudoislets in terms ofinsulin-secretory responses to glucose and amino acids. (portlandpress.com)
  • The aim of the present study was to explore the effects of ultraviolet (UV) and infrared (IR) irradiation of low intensity on cultured cells derived from different biological tissues (spleen, bone marrow, and Ehrlich's adenocarcinoma), which were immobilized in a porous TiNi-based alloy scaffold. (vbripress.com)
  • The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. (elsevier.com)
  • Scanning electron microscopy results reveal that SMCs on nanostructured surfaces are more elongated and that cells are interacting with the nano-features on the surface. (mdpi.com)
  • The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that SMCs on nanostructured surfaces may be differentiated and slowly dividing. (mdpi.com)