Stage-Specific Embryonic Antigens: Cell-surface molecules that exhibit lineage-restricted patterns of expression during EMBRYONIC DEVELOPMENT. The antigens are useful markers in the identification of EMBRYONIC STEM CELLS.Antigens, CD15: A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.Teratoma: A true neoplasm composed of a number of different types of tissue, none of which is native to the area in which it occurs. It is composed of tissues that are derived from three germinal layers, the endoderm, mesoderm, and ectoderm. They are classified histologically as mature (benign) or immature (malignant). (From DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1642)Globosides: Glycosphingolipids containing N-acetylglucosamine (paragloboside) or N-acetylgalactosamine (globoside). Globoside is the P antigen on erythrocytes and paragloboside is an intermediate in the biosynthesis of erythrocyte blood group ABH and P 1 glycosphingolipid antigens. The accumulation of globoside in tissue, due to a defect in hexosaminidases A and B, is the cause of Sandhoff disease.Glycosphingolipids: Lipids containing at least one monosaccharide residue and either a sphingoid or a ceramide (CERAMIDES). They are subdivided into NEUTRAL GLYCOSPHINGOLIPIDS comprising monoglycosyl- and oligoglycosylsphingoids and monoglycosyl- and oligoglycosylceramides; and ACIDIC GLYCOSPHINGOLIPIDS which comprises sialosylglycosylsphingolipids (GANGLIOSIDES); SULFOGLYCOSPHINGOLIPIDS (formerly known as sulfatides), glycuronoglycosphingolipids, and phospho- and phosphonoglycosphingolipids. (From IUPAC's webpage)Glycolipids: Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)Antigens, Tumor-Associated, Carbohydrate: Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.Antigens, Neoplasm: Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Carcinoembryonic Antigen: A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Antigens, Surface: Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Antigens, Viral: Substances elaborated by viruses that have antigenic activity.Organ Specificity: Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Tissue Distribution: Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.Antigens, Protozoan: Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.Antigens, Polyomavirus Transforming: Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.HLA Antigens: Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Antigens, Fungal: Substances of fungal origin that have antigenic activity.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Antigens, Helminth: Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.H-2 Antigens: The major group of transplantation antigens in the mouse.Antigens, Viral, Tumor: Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Mice, Inbred C57BLMice, Inbred BALB CGene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.HLA-DR Antigens: A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.Animals, Genetically Modified: ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.Receptors, Antigen, T-Cell: Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.Histocompatibility Antigens: A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Proliferating Cell Nuclear Antigen: Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Histocompatibility Antigens Class II: Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Prostate-Specific Antigen: A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.O Antigens: The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Receptors, Antigen, B-Cell: IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.Epitopes: Sites on an antigen that interact with specific antibodies.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Tissue Inhibitor of Metalloproteinase-2: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. An overexpression of TIMP-2 has been shown to inhibit invasive and metastatic activity of tumor cells and decrease tumor growth in vivo.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.HLA-A2 Antigen: A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Blood Group Antigens: Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.Hepatitis B Surface Antigens: Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).Larva: Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.HLA-A Antigens: Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Histocompatibility Antigens Class I: Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.HLA-D Antigens: Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Receptors, Antigen: Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.Antigens, CD45: High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.Hepatitis B Antigens: Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Alternative Splicing: A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.Antigens, CD4: 55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Antigen-Antibody Reactions: The processes triggered by interactions of ANTIBODIES with their ANTIGENS.Antigens, CD1: Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.HLA-B Antigens: Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.Antigens, Differentiation: Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.MART-1 Antigen: A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.

*Stem cell

The cell surface antigens most commonly used to identify hES cells are the glycolipids stage specific embryonic antigen 3 and 4 ... into specialized cell types with characteristics consistent with cells of various tissues such as muscles or nerves. Embryonic ... By using human embryonic stem cells to produce specialized cells like nerve cells or heart cells in the lab, scientists can ... Embryonic stem (ES) cells are the cells of the inner cell mass of a blastocyst, an early-stage embryo. Human embryos reach the ...

*Transgenesis

... embryonic cell transfer can be tested at the cell stage. Pharming, a portmanteau of "farming" and "pharmaceutical", refers to ... The manipulated cell is cultured in vitro to develop to a specific embryonic phase, is then transferred to a recipient female. ... typically mesenchymal stem cells from adipose tissue). Transgenic vectors can be delivered randomly[citation needed], or ... antigens) Growth hormones for treatment of deficiencies Factor VIII blood clotting factor Factor IX blood clotting factor ...

*Fucosyltransferase 3

"A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4) ... They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the ... The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial ... CD174 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH). ...

*Regeneration in humans

In humans with non-injured tissues, the tissue is naturally regenerated over time; by default these tissues have new cells ... 2012). "Efficient Derivation of Purified Lung and Thyroid Progenitors from Embryonic Stem Cells". Cell Stem Cell. 10: 398-411. ... An ECM scaffold was created by removing the cells and MHC antigens from a human donated trachea, which was then colonised by ... Through growing patient-specific lung epithelium in vitro, lung tissue expressing the cystic fibrosis phenotype has been ...

*Ovarian cancer

The buildup of extra cells often forms a mass of tissue called a growth or tumor. These abnormal cancer cells have many genetic ... There is no specific marker for immature teratomas, but carcinoembryonic antigen (CEA), CA-125, CA19-9, or AFP can sometimes ... Stage I yolk sac tumors are highly treatable, with a 5-year disease free survival rate of 93%, but stage II-IV tumors are less ... Teratomas are characterized by the presence of disorganized tissues arising from all three embryonic germ layers: ectoderm, ...

*ST3GAL3

... is a stage-specific embryonic antigen-4 synthase". The Journal of Biological Chemistry. 278 (29): 26474-9. doi:10.1074/jbc. ... in cell lines and rat kidney tissue sections: evidence for golgi and post-golgi localization". Glycobiology. 8 (3): 245-57. doi ... Kitagawa H, Paulson JC (Jul 1994). "Differential expression of five sialyltransferase genes in human tissues". The Journal of ... "Structural and mechanistic features of protein O glycosylation linked to CD8+ T-cell apoptosis". Molecular and Cellular Biology ...

*Clonal selection

However, only cells that are tolerant to self tissue will survive the embryonic stage. If non-self tissue is introduced, the ... a specific antigen only activates (i.e. selection) its counter-specific cell so that particular cell is induced to multiply ( ... in the immune system ranging from cells which are tolerant of self tissue to cells which are not tolerant of self tissue. ... able to react with different antigens. When an antigen comes, it binds to a matching side chain. Then the cell stops producing ...

*Secretomics

Secretomic analysis of cancer cell lines or diseased tissue presents a simpler and more specific alternative for biomarker ... Prostate-specific antigen (PSA), the current standard biomarker for prostate cancer, has a low diagnostic specificity - PSA ... This type of analysis could help replace preimplantation genetic screening (PGS), which involves biopsy of embryonic cells and ... currently biomarkers only exist for monitoring later stages of cancer. Secretomic analysis of breast cancer cell lines led to ...

*Biomarker (cell)

... for colorectal cancer and the Prostate Specific Antigen (PSA) for prostate cancer. In 2014, Cancer research identified ... Nagano, K; Yoshida, Y; Isobe, T (2008). "Cell surface biomarkers of embryonic stem cells". Proteomics. 8: 4025-35. doi:10.1002/ ... This is because certain biomarkers within a cell could be altered (most likely in the tertiary stage of their formation) to ... A biomarker, or biological marker, is defined as a "cellular, biochemical or molecular alteration in cells, tissues or fluids ...

*Avian orthoreovirus

Cell-mediated immunity[edit]. An experiment that used monoclonal antibodies that were specific for chick Ia (a chicken class II ... Inoculation of embryonic eggs through the yolk sac has shown that the virus usually kills the embryos within 5 or 6 days post ... The other four tests detect group antigens.[15] Maternal antibodies[edit]. Maternal antibodies have displayed protection ... Once the infection has entered the body, avian reovirus can survive in the tissues of chickens for many weeks. Resistance to ...

*Mir-181 microRNA precursor

In addition, miR-181 family members were highly expressed in the embryonic liver and isolated hepatic stem cells. Especially, ... One selective modulators of estrogen receptor having specific activities of tissue. Tamoxifen acts as an anti-estrogen ( ... The increased expression of miR-181a in mature T cells increases susceptibility to peptide antigens, while inhibiting the ... as compare to low grade stage (Stage 1;2;3 and 4S) whereas mir-181 c and d isoforms are not. In these conditions, they regulate ...

*Collapsin response mediator protein family

5 in the adult testis is detected only in the cell spermatid stage and CRMP-2 mRNA is found in lung tissue of the fetal mouse ... In general, CRMPs are highly expressed in post-mitotic nerve cells since early embryonic life. In the developing nervous system ... In following researches, CRMPs were studied as target antigens for autoantibodies in various autoimmune neurodegenerative ... using a rabbit polyclonal antiserum which recognized a 64 kDa mouse brain specific phosphoprotein. In the same year, several ...

*P19 cell

P19 cells are embryonic carcinoma cell lines derived from an embryo-derived teratocarcinoma in mice. The cell line is ... cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. Indeed, exposing ... The researchers found that there were tissues from all three germ layers growing in the recipient mouse. Based on their ... The extended research can also elucidate the later stages of heart or brain development and maturation. McBurney, MW; Rogers, ...

*Tumor antigen

... cancer-testis antigens and vascular or stromal specific antigens. Tissue differentiation antigens are those that are specific ... These proteins are normally produced in the early stages of embryonic development and disappear by the time the immune system ... Tumor-Specific Antigens (TSA), which are present only on tumor cells and not on any other cell and Tumor-Associated Antigens ( ... and some viral antigens are also cancer antigens. Cancer-testis antigens are antigens expressed primarily in the germ cells of ...

*Cancer stem cell

Stem-like tumor cells have been identified using cell surface markers including CD133, SSEA-1 (stage-specific embryonic antigen ... This is most often associated with tissues with a high rate of cell turnover (such as the skin or gut). In these tissues, ASCs ... although specific cancers have been linked to a specific cause. De-differentiation of mutated cells may create stem cell-like ... "Critical appraisal of the side population assay in stem cell and cancer stem cell research". Cell stem cell. 8 (2): 136-47. doi ...

*Placenta

... of proteins with elevated expression in placenta compared to other organs and tissues are PEG10 and the cancer testis antigen ... There is presence of small lymphocytic suppressor cells in the fetus that inhibit maternal cytotoxic T cells by inhibiting the ... The period from just after the child is born until just after the placenta is expelled is called the "third stage of labor". ... The corresponding specific proteins are mainly expressed in trophoblasts and have functions related to female pregnancy. ...

*Adult neurogenesis

Some antigens can be used to measure specific stem cell stages. For example, stem cells requires the sox2 gene to maintain ... of the cannabinoids results in the growth of new nerve cells in the hippocampus from both embryonic and adult stem cells. In ... "Cells keep a memory of their tissue origin during axolotl limb regeneration". Nature. 460 (7251): 60-65. doi:10.1038/ ... Specific cell types are then determined with unique differences in their expression of proteins, which can be used as antigens ...

*Cellular differentiation

... turning off the expression of embryonic stem cell (ESC) genes, and the activation of cell fate genes. Lysine specific ... The cells of the inner cell mass go on to form virtually all of the tissues of the human body. Although the cells of the inner ... Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, ... The Wnt pathway is involved in all stages of differentiation, and the ligand Wnt3a can substitute for the overexpression of c- ...

*Neuronal lineage marker

All of these antigens are present in specific neuronal cell types. With these we can define anatomical circuits with a high ... Von Bohlen Und Halbach, O (2007). "Immunohistological markers for staging neurogenesis in adult hippocampus". Cell and Tissue ... "Development and differentiation of neural rosettes derived from human embryonic stem cells". Stem Cell Reviews. 2 (1): 67-77. ... The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have ...

*Co-receptor

... embryonic development, cell adhesion regulation, gradient formation, tissue proliferation and migration. The CD family of co- ... Carcinoembryonic antigen cell adhesion molecule-1 (Caecam1) is an immunoglobulin-like co-receptor that aids in cell adhesion in ... The key to optimal cellular functioning is maintained by possessing specific machinery that can carry out tasks efficiently and ... and transmission while those using the CXCR4 receptor do not present until later in the immunologically suppressed stage of the ...

*Testicle

The testes have the highest fraction of tissue type-specific genes compared to other organs and tissues:[8] about 1000 of them ... Peritubular myoid cells surround the seminiferous tubules.[4]. Between tubules (interstitial cells). *Leydig cells - cells ... The Tanner scale for the maturity of male genitals assigns a maturity stage to the calculated volume ranging from stage I, a ... Embryonic. During mammalian development, the gonads are at first capable of becoming either ovaries or testes.[10] In humans, ...

*Rex1

"Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens". Stem ... The only adult tissue Rex1 has been identified in are the testicles. Using in situ hybridization it was determined that the ... Thus, the male germ cells undergoing meiosis are the specific cells in the testicles that express Rex1. It has not been ... Also, Rex1−/Oct3/4+ cells differentiate into cells of primitive ectoderm, the somatic cell lineage. Studies have shown that ...

*Glypican

GPC1 plays a role in heparin-binding and cell cycle progression in the breast tissue. Glypican-2 (GPC2) is a cell surface ... Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 have been made. Immunotoxin treatment inhibits ... Furthermore, oftentimes GPC3 expression occurs during embryonic development in these tissues, and is subsequently re-expressed ... The regulating processes performed by glypicans can either stimulate or inhibit specific cellular processes. The mechanisms by ...

*HOXA11-AS1

HOXA11-AS has been shown to be significantly up-regulated in gastric cancer tissue cells. The increase in expression of HOXA11- ... Given the sparse research on the specific mechanism of HOXA11-AS lncRNA and what is known about the role of HOX genes, it may ... The HOX genes code for transcription factors that are key to embryonic development. This lncRNA is one of the three lncRNAs ... Correlations were seen between patients with larger tumor size, higher carcinoembryonic antigen (CEA), and increased metastases ...

*Amniotic stem cells

Embryonic-like stem cells were originally identified using markers common to embryonic stem cells such as nuclear Oct4, CD34, ... Injection of cells immediately following the infarct is particularly beneficial as the cells protect the cardiac tissue from ... these results may be due to the specific features of the NRVM or fusion of the cells rather than the amniotic stem cell's own ... "Preclinical regulatory validation of a 3-stage amniotic mesenchymal stem cell manufacturing protocol". Journal of pediatric ...

*Genetic engineering

Embryonic stem cells incorporate the altered gene, which replaces the already present functional copy. These stem cells are ... In 2017, genetic engineering of chimeric antigen receptors on a patient's own T-cells was approved by the U.S. FDA as a ... Narayanaswamy, S. (1994). Plant Cell and Tissue Culture. Tata McGraw-Hill Education. pp. vi. ISBN 9780074602775. Health, ... so researchers can easily determine which cells have been successfully transformed. The gene can also be modified at this stage ...

*Large-cell lung carcinoma with rhabdoid phenotype

... lacking specific cytological and tissue architectural characteristics of other types, subtypes, and variants of lung cancer. ... More than 99% of primary lung cancers are carcinoma, which are tumors composed of cells that originate from embryonic ectoderm ... "Histotype in non-small cell lung cancer therapy and staging: The emerging role of an old and underrated factor". Curr Resp Med ... Typically, markers expressed in LCLC-RP include those seen in "generic" NSCLC's, such as epithelial membrane antigen (EMA, 61 ...
Lipid rafts enriched in glycosphingolipids (GSLs), cholesterol and signaling molecules play an essential role not only for signal transduction started by ligand binding, but for intracellular events such as organization of actin, intracellular traffic and cell polarity, but their functions in cleavage division of preimplantation embryos are not well known. Here we show that monosialylGb5Cer (MSGb5Cer)-enriched raft domains are involved in development during the cleavage stage of mouse preimplantation embryos. MSGb5Cer preferentially localizes at the interfaces between blastomeres in mouse preimplantation embryos. Live-imaging analysis revealed that MSGb5Cer localizes in cleavage furrows during cytokinesis, and that by accumulating at the interfaces, it thickens them. Depletion of cholesterol from the cell membrane with methyl-beta-cyclodextrin (MbCD) reduced the expression of MSGb5Cer and stopped cleavage. Extensive accumulation of MSGb5Cer at the interfaces by cross-linking with ...
Clone 8e11 recognizes the human stage-specific embryonic antigen-5 (SSEA-5) antigen, an oligosaccharide that contains the motif Fucα1-2Galβ1-3GlcNAcβ, also known as the H type-1 (H-1) antigen. SSEA-5 is highly and specifically expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is also expressed on the surface of cells in the inner cell mass of the human blastocyst during embryogenesis and expression is rapidly down-regulated upon cellular differentiation. - USA
Clone 8e11 recognizes the human stage-specific embryonic antigen-5 (SSEA-5) antigen, an oligosaccharide that contains the motif Fucα1-2Galβ1-3GlcNAcβ, also known as the H type-1 (H-1) antigen. SSEA-5 is highly and specifically expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is also expressed on the surface of cells in the inner cell mass of the human blastocyst during embryogenesis and expression is rapidly down-regulated upon cellular differentiation. - USA
TY - JOUR. T1 - Characterization and multilineage differentiation of embryonic stem cells derived from a buffalo parthenogenetic embryo. AU - Sritanaudomchai, Hathaitip. AU - Pavasuthipaisit, Kanok. AU - Kitiyanant, Yindee. AU - Kupradinun, Piengchai. AU - Mitalipov, Shoukhrat. AU - Kusamran, Thanit. PY - 2007/10/1. Y1 - 2007/10/1. N2 - Embryonic stem (ES) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. We herein describe production of parthenogenetic buffalo (Bubalus Bubalis) blastocysts and subsequent isolation of an ES cell line. Established parthenogenetic ES (PGES) cells exhibited diploid karyotype and high telomerase activity. PGES cells showed remarkable long-term proliferative capacity providing the possibility for unlimited expansion in culture. Furthermore, these cells expressed key ES ...
Retinal axons undergo several changes in organization as they pass through the region of the optic chiasm and optic tract. We used immunocytochemistry to examine the possible involvement of fibroblast growth factor receptors (FGFR) in these changes in retinal axon growth. In the retina, at all ages examined, prominent staining for FGFR was seen in the optic fiber layer and at the optic disk. At embryonic day 15 (E15), FGFR immunoreactivity was also detected in the ganglion cell layer, as defined by immunoreactivity for islet-1. At later developmental stages (E16 to postnatal day 0), FGFR were found in the optic fiber layer and the inner plexiform layer. In the ventral diencephalon, immunostaining for FGFR was first detected at E13 in a group of cells posterior to the chiasm. These cells appeared to match the neurons that are immunopositive for the stage-specific embryonic ...
Bacterial carbohydrate structures play a central role in mediating a variety of host-pathogen interactions. Glycans can either elicit protective immune response or lead to escape of immune surveillance by mimicking host structures. Lipopolysaccharide (LPS), a major component on the surface of Gram-negative bacteria, is composed of a lipid A-core and the O-antigen polysaccharide. Pathogens like Neisseria meningitidis expose a lipooligosaccharide (LOS), which outermost glycans mimick mammalian epitopes to avoid immune recognition. Lewis X (Gal beta 1-4(Fuc alpha 1-3)GlcNAc) antigens of Helicobacter pylori or of the helminth Schistosoma mansoni modulate the immune response by interacting with receptors on human dendritic cells. In a glycoengineering approach we generate human carbohydrate structures on the surface of recombinant Gram-negative bacteria, such as Escherichia coli and Salmonella enterica sv. Typhimurium that lack O-antigen. A ubiquitous building block in mammalian ...
Currently different in vitro experimental approaches make use of the unique potential of pluripotent cells in order to produce functional sperm aiming at the establishment of new treatment opportunities for male infertility. The establishment of proliferating human spermatogonial stem cell (SSC) lines and their in vitro differentiation into haploid male germ cells represents one of these approaches and was tried to succeed in the presented thesis. The isolation of human SSCs from testicular biopsies from infertile men was performed using the Magnetic Activated Cell Separation technique and succeeded several times in the enrichment of putative SSCs, characterized by their grape-like structures characteristically for SSCs and a positive SSC marker staining. Despite of numerous attempts and testing of different culture conditions, a stable and proliferating human SSC cell line could not be established. Because other studies also revealed great difficulties in the establishment ...
CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 is a carbohydrate adhesion molecule that can be expressed on glycoproteins, glycolipids and proteoglycans. CD15 mediates phagocytosis and chemotaxis, found on neutrophils; expressed in patients with Hodgkin disease, some B-cell chronic lymphocytic leukemias, acute lymphoblastic leukemias, and most acute nonlymphocytic leukemias. It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem cells, in which it plays an important role in adhesion and migration of the cells in the preimplantation embryo. It is synthezised by FUT4 (fucosyltransferase 4) and FUT9. CD15 is present on almost all Reed-Sternberg cells, including their rare mononuclear variants, and, as such, can be used in immunohistochemistry to ...
Abcam provides specific protocols for Anti-Carcino Embryonic Antigen CEA antibody [COL-1] (ab105364) : Flow cytometry protocols, Immunoprecipitation protocols…
Mouse monoclonal Carcino Embryonic Antigen CEA antibody [NCRC16 (AKA 161)] validated for ELISA, IHC, ICC/IF and tested in Human. Referenced in 1 publication…
Purpose : Mesenchymal stem cell (MSC)-based therapy is under investigation as a treatment strategy to promote tissue repair and to modulate the immune system in immune mediated disorders. MSCs have been isolated from many tissues most commonly from the bone marrow or fat. Given the cumbersome procedures for harvesting bone marrow (BM), and to a lesser extent fat, there is a need to look for other tissues for MSC isolation. The International Society for Cellular Therapy (ISCT) has established three criteria for characterizing human MSCs: adherence to plastic, differentiation potential, and the presence or absence of certain cell surface markers. We evaluated whether or not corneal limbal stromal cells (CLSC) met ISCT criteria by comparing them to BM-MSCs. Methods : BM-MSCs were isolated from healthy human bone marrow donors. CLSC were isolated from human corneal limbus from research cadaver corneas (generously provided by Eversight). Both cell types were ...
FIGURE 1. In vivo Ab production after the intranasal immunization with saline, HSA or sugar-conjugated HSA in BALB/c (A-D) and C57BL/6 (E and F) mice. Serum samples were taken after prime (▦), boost (□), and challenge (▪) as described in Materials and Methods. Ab of IgG (A and E), IgM, IgA, and IgG subclasses (B) specific for HSA were measured by indirect ELISA and estimated by endpoint titer. IgE titers specific for HSA (C and F) and the volume of serum total IgE (D) were determined by sandwich ELISA. Levels of HSA-specific IgE were presented as the absorbance at 450 nm from duplicate wells of 1/4 serum dilution. Results show the mean ± SEM of four individual serum per group. Data are representative of three (A-D) and two (E and F) separate experiments.. ...
Quantity100 testsVolume1ImmunogenHuman granulocytesBackground InformationCD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a t...
TY - JOUR. T1 - Surface Marker Epithelial Cell Adhesion Molecule and E-cadherin Facilitate the Identification and Selection of Induced Pluripotent Stem Cells. AU - Chen, Hsin Fu. AU - Chuang, Ching Yu. AU - Lee, Wen Chih. AU - Huang, Hsiang Po. AU - Wu, Han Chung. AU - Ho, Hong Nerng. AU - Chen, Yu Ju. AU - Kuo, Hung Chih. PY - 2011/9/1. Y1 - 2011/9/1. N2 - The derivation of induced pluripotent stem cells (iPSCs) requires not only efficient reprogramming methods, but also reliable markers for identification and purification of iPSCs. Here, we demonstrate that surface markers, epithelial cells adhesion molecule (EpCAM) and epithelial cadherin (E-cadherin) can be used for efficient identification and/or isolation of reprogrammed mouse iPSCs. By viral transduction of Oct4, Sox2, Klf4 and n- or c-Myc into mouse embryonic fibroblasts, we observed that the conventional mouse embryonic stem cell (mESC) markers, alkaline phosphatase ...
Antigens: an, Brain: em, im, Endometrium: im, Epididymis: im, Fallopian-Tubes: im, Female, Fetus: im, Germ-Layers: im, Gestational-Age, Glycolipids: an, Kidney: em, im, Male, Mice: em, Postimplantation-Phase, Pregnancy, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Teratoma: im. ...
TY - CONF. T1 - Extractables and Leachables from Filter Material and their Effect on Human Granulocyte Colony-Stimulating Factor (hG-CSF). AU - Wahl, Verena. AU - Mohr, Stefan. AU - Wieser, Juliana. AU - Khinast, Johannes. AU - Paudel, Amrit. PY - 2015/11/11. Y1 - 2015/11/11. M3 - (Altdaten) Vortrag oder Präsentation. ER - ...
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Our studies demonstrate that H. pylori LPS stimulates the release of both neutrophil-activating, C-X-C chemokines (IL-8 and ENA-78) and the monocyte-activating C-C chemokine MCP-1 from human monocytes. These chemokines are potent leukocyte chemoattractants and may play an important role in regulating inflammatory cell infiltration of H. pylori-infected gastric mucosa (7, 11, 14, 15,20, 21, 23, 27, 37, 40, 48). We found that H. pyloriLPS is less potent than Salmonella lipid A in inducing monocyte chemokine production. This finding agrees with previous studies showing low potency for H. pylori LPS in the induction of a wide variety of host inflammatory responses (9, 18,19, 34, 36, 38, 39, 42). However, when the actions of H. pylori LPS were specifically inhibited by using either an LPS antagonist or CD14 receptor blockade, the monocyte-activating potential of H. pylori water extract was almost completely abolished. These findings suggest that H. pylori LPS may be the primary ...
TY - JOUR. T1 - Sialyl Lewis X-dependent lung colonization of B16 melanoma cells through a selectin-like endothelial receptor distinct from E- or P-selectin. AU - Zhang, Jianing. AU - Nakayama, Jun. AU - Ohyama, Chikara. AU - Suzuki, Masami. AU - Suzuki, Atsushi. AU - Fukuda, Minoru. AU - Fukuda, Michiko N.. PY - 2002/8/1. Y1 - 2002/8/1. N2 - Endothelial carbohydrate binding proteins, E- and P-selectins, are thought to mediate sialyl Lewis A/X-dependent hematogenous cancer metastasis. We tested this hypothesis using sialyl Lewis X-dependent B16 melanoma lung targeting and its inhibition with selectin ligand mimicry peptide, IELLQAR. In E/P-selectin doubly deficient mutant mice, sialyl Lewis X-expressing B16 melanoma cells colonized the lung, and IELLQAR inhibited this colonization. However, tumors grown in E/P-selectin-deficient mice were significantly smaller than those grown in wild-type mice. These results indicate that the IELLQAR peptide receptor expressed in the lung ...
Helicobacter pylori persistently colonizes about half the human population and contributes to the development of peptic ulcer disease and gastric cancer. This organism has evolved means to structurally alter its surface characteristics to evade innate and adaptive immune responses. H. pylori produces LIPS O-antigen units that can be posttranslationally fucosylated to generate Lewis antigens, structures also found on human epithelial cells. We demonstrate an extensive diversity of Lewis x and Lewis y expression in LPS O-antigen units, occurring over time and in different regions of the human stomach. Lewis expression patterns were correlated with the on/off status of the three fucosyltransferases (FucT), FutA, FutB, and FutC, which are regulated via slipped-strand mispairing in intragenic polyC tract regions of the corresponding genes. The alpha 1,3-FucT, FutA and FutB, each contain a C-terminal heptad repeat region, consisting of a variable number of DD/NLRV/INY tandem ...
Transcripts of α-(1,3)-fucosyltransferases in human epithelial cancer and leukemia cell lines were analyzed by Northern blotting and reverse transcriptase mediated-polymerase chain reaction using specific probes and primers which can discriminate between the transcripts derived from the four α-(1,3)-fucosyltransferase genes Fuc-TIII, IV, V, and VI. Flow cytometric analysis of the sialyl Lex and sialyl Lea antigens was also performed on the same cell lines. The sialy Lex antigen was expressed on 14 of 15 epithelial cancer cell lines, and the sialyl Lea antigen was detected on 8 of them. The message of Fuc-TIII was detected in most of the epithelial cancer cell lines (14 of 15), which correlated with the surface expression of these carbohydrate determinants. In addition, the messages of Fuc-TIV and Fuc-TVI were detected in most epithelial cancer cell lines, while the message of Fuc-TV was undetectable in most of them. On the other hand, all leukemia cell lines ...
The α-(1,2) fucosyltransferases (Fut1 and Fut2) and α-(1,3) fucosyltransferases (Fut4, Fut9) are responsible for the synthesis of Lewis X (LeX) and Lewis Y (LeY) conjugated to glycoproteins. We recently reported that these fucosyltransferases were differentially expressed in the reproductive tract of male mouse. Here, we studied the effect of androgen on fucosyltransferase expression through the use of mouse castration models. We found that Fut1 mRNA and Fut4 mRNA were upregulated, while Fut2 mRNA and Fut9 mRNA were downregulated by androgen in the caput epididymis. However, in the vas deferens and prostate, only Fut4 mRNA and Fut2 mRNA were respectively upregulated following exposure to androgen. In the seminal vesicle, all fucosyltransferases, with the exception of Fut9, were upregulated. We identified the androgen receptor binding sites (ARBSs) of Fut2, Fut4 and Fut9 in the caput epididymis. Luciferase assay for these ARBSs is able to provide an indication as to why Fut4 and Fut9 are differently
Results Mesodermal markers increased in EB while undifferentiated ES markers disappeared. After 21 days of chondrogenic culture in micromass pellets, GAG analysis showed that proteoglycan production was significantly greater in chondrogenic pellets than in undifferentiated hiPSCs and EBs. Safranin-O staining demonstrated that the cells in chondrogenic pellets took on the appearance of immature chondrocytes and secreted extracellular matrix. The chondrogenic marker gene and protein expression increased after 21days of pellet culture. The chondrogenic pellets derived from hiPS cells have very low expression of hypertrophic or osteogenic markers.. Also, hiPS cells underwent good chondrogenic differentiation in PLGA scaffold or alginate gel as well. When hiPS cells in either pellet state or in alginate hydrogel were implanted in the osteochondral defects created on the patellar groove of immunosuppressed rats, the defects implanted with ...
Looking for online definition of ELAM ligand fucosyltransferase in the Medical Dictionary? ELAM ligand fucosyltransferase explanation free. What is ELAM ligand fucosyltransferase? Meaning of ELAM ligand fucosyltransferase medical term. What does ELAM ligand fucosyltransferase mean?
Cell replacement therapy could be an important treatment strategy for Parkinsons disease (PD), which is caused by the degeneration of dopamine neurons in the midbrain (mDA). The success of this approach greatly relies on the discovery of an abundant source of cells capable of mDAergic function in the brain. With the paucity of available human fetal tissue, efforts have increasingly focused on renewable stem cells. Human induced pluripotent stem (hiPS) cells offer great promise in this regard. If hiPS cells can be differentiated into authentic mDA neuron, hiPS could provide a potential autologous source of transplant tissue when generated from PD patients, a clear advantage over human embryonic stem (hES) cells. Here, we report that mDA neurons can be derived from a commercially available hiPS cell line, IMR90 clone 4, using a modified hES differentiation protocol established in our lab. These ...
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dyes functional group. See the product information sheets for specific reactive dyes more information.. Coelenterazines and D-luciferin. Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. ...
With Cellartis clinical-grade hES cell line derivation services, we will deliver clinical-grade human ES cell lines for use in clinical research settings. The proprietary hES cell establishment method is feeder-free and animal/human-component free. The starting material is retrieved from FDA-compliant sources according to FDA guidelines. For more information, visit the services overview page and fill out the inquiry form on the right-hand side.. ...
Recent studies suggest that ovarian germ line stem cells replenish oocyte-pool in adult stage, and challenge the central doctrine of fixed germ cell pool in mammalian reproductive biology. Two distinct populations of spherical stem cells with high nucleo-cytoplasmic ratio have been recently identified in the adult mammalian ovary surface epithelium (OSE) including nuclear OCT-4A positive very small embryonic-like (VSELs) and cytoplasmic OCT-4 expressing ovarian germ stem cells (OGSCs). Three weeks culture of scraped OSE cells results in spontaneous differentiation of the stem cells into oocyte-like, parthenote-like, embryoid body-like structures and also embryonic stem cell-like colonies whereas epithelial cells attach and transform into a bed of mesenchymal cells. Present study was undertaken, to further characterize ovarian stem ...
Sialyl Lewis X-i antigen has been used as a tool for the clinical assessment of lung cancers, especially lung adenocarcinoma. However, the carbohydrate antigens including sialyl Lewis X-i antigen are also increased in some patients with non-malignant lung diseases such as IPF without coexistent malignancy.8-12 There is as yet no test to make a specific diagnosis in patients with raised levels of the antigen. When increased serum levels of the antigen are found in patients with IPF without a suspicious mass on the chest CT scan, further invasive and costly tests are often considered to rule out adenocarcinoma originating from other organs. If the core proteins of the antigen in patients with lung adenocarcinoma are not the same as those in patients with IPF, it is expected that the pattern of expression would differ on Western blotting analysis. In order to establish the specific test for a differential diagnosis we evaluated ...
Semantic Scholar extracted view of Independently of Cutaneous Lymphocyte of Skin-Homing Properties Occurs E-Selectin Binding CD4 T Cells: Acquisition In Vitro Differentiation from Naive to Mature by Tetsuo Shiohara et al.
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References: Ahuja, Y.R., Vijayalakshmi, V., Stem cell test: A practical tool in toxicogenomics (2007) Toxicology, 231 (1), pp. 1-10; Ainsworth, C.D., Crowther, M.A., Treleaven, D., Evanovttch, D., Webert, K.E., Blajchman, M.A., Severe hemolytic anemia post-renal transplantation produced by donor anti-D passenger lymphocytes, case report and literature review (2009) Transfusion Medical Review, 23 (2), pp. 155-159; Amit, M., Carpenter, M.K., Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture (2000) Developmental Biology, 227 (2), pp. 271-278; Amit, M., Itskovitz-Eldor, J., Maintenance of human embryonic stem cells in animal serum- and feeder layer-free culture conditions (2006) Methods Molecular Biology, 331, pp. 105-113; Amit, M., Margulets, V., Human feeder layers for human embryonic stem cells (2003) Biological Reproduction, 68 (6), pp. ...
Chronic Helicobacter pylori infection is recognized as a cause of gastric cancer. H. pylori adhesion to gastric cells is mediated by bacterial adhesins such as sialic acid-binding adhesin (SabA), which binds the carbohydrate structure sialyl-Lewis x. Sialyl-Lewis x expression in the gastric epithelium is induced during persistent H. pylori infection, suggesting that H. pylori modulates host cell glycosylation patterns for enhanced adhesion. Here, we evaluate changes in the glycosylation-related gene expression profile of a human gastric carcinoma cell line following H. pylori infection. We observed that H. pylori significantly altered expression of 168 of the 1,031 human genes tested by microarray, and the extent of these alterations was associated with the pathogenicity of the H. pylori strain. A highly pathogenic strain altered expression of several genes involved in glycan biosynthesis, in particular that encoding β3 GlcNAc T5 (β3GnT5), a GlcNAc transferase essential for the biosynthesis ...
Characterisation of cornerstone adhesions. a Spinning disk images of hPSC plated on vitronectin (VTN) and stained for F-actin, paxillin, DAPI, and the pluripotency marker Nanog. Scale bar 10 μm. b Structured illumination microscopy images of hPSC plated on VTN and stained for filamentous actin (F-actin) and paxillin. Scale bar 10 μm. The white square highlights a region of interest (ROI), which is magnified. c-f Live-cell imaging of endogenously tagged paxillin in hPSC. Images were acquired every minute using a spinning disk microscope. Colonies were recorded for at least 105 min (n = 16 independent colonies from three biologically independent experiments). A representative video is available as supplementary information (Supplementary Movie 1). c A representative image is displayed (ROI highlighted by white square and magnified; size, 30 µm) in addition to a mask image highlighting paxillin-positive adhesions (colony edge FA, green; colony centre FA, magenta) and merged images depicting ...
I n "The Quest for Innovative Science," eminent in vitro fertilization (IVF) specialist and founder scientist of Embryonic Stem Cell International (ESI), Professor Ariff Bongso, shares the story of his scientific endeavors. These include several "firsts" and the establishment of ESI, which aims to become the world leading provider of products and technologies derived from human embryonic stem cells. Bongso has authored 410 publications in refereed journals, including the paper "Embryonic Stem Cell Lines from Human Blastocysts: Somatic Differentiation in vitro" in Nature Biotechnology , 18, pp. 399-404 (2000), and chapters in books. He also holds patents on the ?Isolation and development of human embryonic stem cell lines? (1999) and ?Derivation and propagation of embryonic stem cells on human feeders and feeder-free matrices? (2001). Starting From Veterinary Medicine Born in Colombo, Sri ...
Human iPS cells cultured with the DEF-CS system are typically 98% Oct-4 positive, as determined by flow cytometry. They also express common pluripotency markers, including the transcription factors Oct-4 and NANOG and the surface markers SSEA-4, TRA-1-60, and TRA-1-81. The cells do not express the differentiation marker SSEA-1. Cells grown in the DEF-CS system have been shown to differentiate in vitro and in vivo into all three germ layers.. ...
In this article, we successfully isolate Sca-1+ cells from postnatal mice hearts with the MACS system, using a microbead-labeled anti-Sca-1 antibody. The isolated Sca-1+ cells express the stemness markers Nanog and TERT, the cardiac mesoderm markers ISL-1, TBX5, and the cardiac specific transcription factors GATA4, Nkx2.5, and MEF2C. They can be propagated in vitro for a long time without any significant changes in marker expression. Moreover, the isolated Sca-1+ cells and their long-term descendants exhibit multipotent differentiation to cardiac cell lineages, including smooth muscle, endothelial cells and cardiomyocytes. So, the Sca-1+ cells we isolated from mouse heart tissue should be identified as CPCs.. Isolation of a cardiac progenitor cell (CPC) population from the heart is challenging, particularly because of a lack of specific surface markers. ...
TY - JOUR. T1 - The antitumor monoclonal antibody MOv2 recognizes the Lewis A hapten. AU - Leoni, F.. AU - Magnani, J. L.. AU - Miotti, S.. AU - Canevari, S.. AU - Pasquali, M.. AU - Sonnino, S.. AU - Colnaghi, M. I.. PY - 1988. Y1 - 1988. N2 - Monoclonal antibody MOv2, produced against ovarian carcinoma, was previously found to bind a carbohydrate epitope (CAMOv2) present on mucins, glycoproteins and a neutral glycolipid. In this paper, the structure of the carbohydrate epitope is determined by immunological reactivity with purified glycolipids and oligosaccharides. Using solid-phase radioimmunoassay and immunostaining of thin layer chromatograms, MOv2 binds strongly to Le(a)-active pentasaccharide ceramide. A smaller neutral glycolipid also weakly binds MOv2. Fifty percent inhibition of binding to Le(a)-active pentasaccharide ceramide is achieved with approximately 8 μM concentration of lacto-N-fucopentaose II (LNF II). Lacto-N-tetraose (LNT) also partially inhibits at about 103 times higher ...
ESI BIOs hES cell lines are among the best-characterized and documented lines available today. These research grade cell lines have also been approved by the National Institutes of Health (NIH) and are included in the NIH Stem Cell Registry.
Human Embryonic Stem Cell Lines For the human ESC cell lines, only those listed on the NIH Stem Cell Registry can be acquired. Note that some lines are on temporary hold, and in addition, some have restrictions as to their use, indicated in Details to the left of the name of the line. If you identify a line you are interested in, contact the Investigator to see if it is available, who to contact about having it shipped, and what the cost would be.
Labial salivary gland biopsy (LSGB) is the most important diagnostic tool for the diagnosis of Sjögrens syndrome (SS), but its diagnostic value is rarely studied. This study assessed the sensibility and specificity of LSGB, and the clinical profiles of patients who were referred for biopsy. Retrospective analysis of the histopathological reports from LSGB and medical report data from patients who underwent LSGB between 2008 and 2011 was conducted. About 290 biopsies were performed and 74 were excluded due to insufficient clinical data. Of the 216 patients, 0.46% was carrier of hepatitis C virus, 30.1% had primary SS (pSS), and 8.8% had secondary SS (sSS). Of the samples, 94.3% presented dryness symptoms, 51.6% experienced dryness only, 42.7% had systemic manifestations, and 66.9% presented low unstimulated salivary flow and/or Schirmers test. LSGB was necessary in 67.6% to confirm the presence of SS based on the American-European Consensus Group 2002 criteria (AECG). Based ...
Expression of CD137, a member of the tumor necrosis factor receptor family, is inducible on vascular endothelial cells by proinflammatory stimuli. Its ligand is expressed as a transmembrane protein on the surface of monocytes, and transmits activating signals into monocytes (reverse signaling) inducing monocyte migration. These findings led to the hypothesis that CD137 expression on activated endothelial cells facilitates recruitment of monocytes into inflammatory tissues including atherosclerotic lesions. Data from this study demonstrate that CD137 expression is inducible on endothelial cells by TNF stimulation. Recombinant CD137 protein and CD137 expressed on activated endothelial cells enhance ICAM-1-mediated adhesion of monocytes under defined flow conditions in vitro. CD137 seems not to play a role in tethering of monocytes since this activity is completely E-selectin-dependent. In addition, LFA-1 affinity and ...
E-selectin is an inducible cell adhesion molecule which mediates rolling of neutrophils as well as adhesion of carcinoma cells to endothelium. Inhibition of selectin-mediated interaction is a possible means for controlling inflammation induced diseases and metastatic spread. The purpose of these studies is to identify peptide forms mimicking Lewis X (LeX), sialyl-Lewis X (SA-LeX), sialyl-Lea (SA-Lea), and Lewis Y (LeY) carbohydrate structures to disrupt lectin-ligand interactions and evaluate their properties to block adenocarcinoma cell adhesion to endothelial cells and tumor metastasis in vitro and in vivo. We used recombinant peptide library to identify novel ligands for E-selectin. We have identified five dodeca peptides mimicking SA-Lea carbohydrate, which is one of the E-selectin ligands, via means of random peptide library screening using SA-Lea-specific monoclonal antibody (MAb) NS 19-9. Peptides with the highest binding affinity for ...
Detects SSEA-1 expression in live, unfixed pluripotent stem cell populations by immunocytochemical staining. Live staining from esibio.com.
Differentiation in murine erythroleukemic cells is arrested at the proerythroblast stage. A small fraction of the population, however, undergoes spontaneous differentiation. This spontaneous differentiation was examined at the individual cell level in relation to cell multiplication, commitment, and maturation. The results indicate that murine erythroleukemic cells destined to undergo spontaneous differentiation first undergo commitment, an irreversible process characterized by cells becoming (a) capable of producing hemoglobin coupled with (b) a loss of their ability to undergo more than six subsequent cell divisions. Commitment is followed by a maturation process which includes the accumulation of erythroid specific markers, e.g., hemoglobin. With respect to commitment, spontaneous differentiation resembles the differentiation produced by most inducers but differs from that evoked by hemin. Serum hemin may ...
Spontaneous differentiation of PBMC CD8αα+CD3− cells into DC-like cells after a short-term in vitro culture(a) Phase-contrast micrographs show morphological
3871 Sialyl Lewis x (sLex) antigen is a carbohydrate antigen that is considered not only a marker for cancer but also implicated functionally in the malignant behaviour of cancer cells. Overexpression of sLex is associated with enhanced progression and metastases of many types of cancer including those of the mammary gland. Canine malignant mammary tumours can invade and give rise to metastases via either lymphatic or blood vessels.. E-Cadherin is specifically involved in epithelial cell-to-cell adhesion. In cancer, E-Cadherin underexpression is one of the alterations that characterizes the invasive phenotype and is considered a potent invasion/tumour suppressor gene. Partial or complete loss of E-Cadherin expression correlates with poor prognosis in canine malignant mammary cancer.. The aim of this study was to analyse the sLex expression in canine malignant mammary tumours and to evaluate if the presence of sLex correlates with the expression of E-Cadherin ...
In article ,3lrio2$ig6 at news.duke.edu,, srps at galactose.mc.duke.edu (Steven Pirie-Shepherd) says: , ,I need to know the structure of the tetrasaccharide sialyl Lewis ^x. I NeuAc(alpha2-3)Gal(beta1-4)GlcNAc(alpha1-3)Fucose. I think. ================================================================ Bob Lauder R.Lauder at lancaster.ac.uk Division of Biological Sciences Tel +(44)(0)1524 65201 Lancaster University, Lancaster, UK Fax +(44)(0)1524 843854 ...
Page contains details about horseradish peroxidase/anti-alpha fetoprotein monoclonal antibody-grafted gold nanoparticles:horseradish peroxidase/anti-carcino embryonic antigen monoclonal antibody-grafted gold nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Recent research and the current scenario as well as future market potential of "​DIAsource ImmunoAssays SA - Product Pipeline Analysis, 2017 Update" globally.. ​DIAsource ImmunoAssays SA (DIAsource), formerly BioSource Europe SA, a subsidiary of Anteo Diagnostics Ltd is a diagnostics company that develops, manufactures and markets clinical diagnostic products. The companys products comprise immunoassays, antibodies, instruments and RUO products. Its antibodies comprise osteocalcin, adrenocorticotropic hormone, adiponectin, aggrecan, calcitonin, alpha-fetoprotein, parathyroid hormone, carcino embryonic antigen, placental lactogen and thyroid stimulating hormone. DIAsources instruments comprise neptune instrument, automated system, ELISA reader, shaker, and washer. The company offers products in the fields of cardiovascular and salt balance, auto-immunity, gastrointestinal metabolism and fertility. It markets products in Belgium and abroad. DIAsource is headquartered in Louvain-La-Neuve, ...
Cells that were aligned via their DNA centroid (see 1.) were rotated based on either the DNA or their cell boundary contours depending on their specific cell cycle category. For cells in interphase and prophase, the alignment contour was based on the two-dimensional cell membrane contour of the center slice of the z-stack of the cell. For cells in metaphase and anaphase, the alignment contour was based on the two-dimensional maximum intensity projection of the z-stack corresponding to the DNA. For cells in early prometaphase, the alignment contour was generated using one of the two techniques just described depending on the eccentricity of the largest connected component of the DNA. For instance, if that value were below 0.55 the cell would be aligned similar to cells in interphase and prophase; however, if the eccentricity exceeded 0.55, indicating a preferential direction for DNA, the cell ...
Schmidl, Martina, Adam, Nadia, Surmann-Schmitt, Cordula, Hattori, Takako, Stock, Michael, Dietz, Uwe, de Crombrugghe, Benoit, Poschl, Ernst and von der Mark, Klaus (2006) Twisted gastrulation modulates BMP- induced collagen II and X expression in chondrocytes in vitro and in vivo. Journal of Biological Chemistry, 281. pp. 31790-31800. ISSN 1083-351X Full text not available from this repository. (Request a copy ...
... : The cells were biopsied directly from the primary tumors of the patients diagnosed with the embryonal carcinoma of the testes (huECT bio) (in the figure 3, the patients encoded 001-009), the mononuclear cells from bone marrow (huBMMC) and from peripheral blood (huPBMC), the cultured human embryonic stem cell lines (H1, H13, H14) (huESC cc), and the cultured cells from metastasis to lungs of the testicular embryonal carcinoma (NT2D1) (huECT cc), labeled with the superparamagnetic Fvs targeting TRA-1-6- and SSEA-4, and isolated with magnetic sorter to enrich the samples purity better than 99.5% with the statistical significance accepted at p , 0.001 ...
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-3 (+); SSEA-4 (+); TRA-1-60 (+); TRA-1-81
... : SSEA-4 is expressed in cells of human testis that have an active POU5F1 promoter. (A): Stably transfected human germ cells with a GFP reporter plasmid under the control of the POU5F1 promoter. Cells expressing GFP (green, shown by the arrows) were used to assay the expression of SSEA-4 in (B). Nuclei are stained by DAPI (blue). Scale bars = 5μm. (B): qRT-PCR analysis of the expression of POU5F1, cMYC and SSEA-4 genes in the following cell populations: Testis is whole human testis cell suspension, SSCs are the POU5F1 promoter-GFP expressing cells cultured under SSC maintenance conditions, gPS are the germline pluripotent cells generated when the GFP expressing cells are cultured under de-differentiation conditions for 5 weeks, iPSCs are the IMR90-4 induced pluripotent stem cells used as a control. The different samples were assayed in triplicates and normalized to GAPDH. Asterisks show ...
6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry. (2008 ...
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Mouse embryonic fibroblasts (MEFs) have been used as feeder cell layers for the culture of Human Embryonic Stem (hES) cells. MEFs could promote optimum growth and prevent differentiation of hESCs. This video describes how to thaw MEF cells and seed in plate for usage. - Thawing Inactivated Feeder Cells (MEFs) - AbVideo™ - Support - Abnova
Initial attempts to end-label polymers bearing highly polar substituents . (a) Jurkat cell labeled with a fluorescent anti-L-selectin antibody, FITC-DREG-56. added to a solution of triethylene glycol (1; 8.9 ml, 66.6 mmol) in 50% aq NaOH (5.3 ml), .. Sialyl Lewis x liposomes as a multivalent ligand and inhibitor of E-selectin. ...
An automatic procedure for writing Lewis electron dot structures, ch3cooch3 lewis structure, writing lewis structures, lewis electron dot structure of ch3cooch3, acetate lewis structure resonance, nail polish removers, weakly polar, lipophilic solvent, resins, major hybrid contributor, resonance structures of methyl acetate, electronegative atom, charge separation in methyl acetate, methyl methanoate lewis structure, ch3cooch3 lewis strucutre, CH3COOCH3 - draw i for, 路易斯结构简单的方法, estructuras de Lewis,Lewis yapıları, methyl acetate, methyl ethanoate,
Apolipoprotein B antibody [F2C3] (apolipoprotein B (including Ag(x) antigen)) for ELISA, IP, RIA. Anti-Apolipoprotein B mAb (GTX15665) is tested in Human samples. 100% Ab-Assurance.
Induction of cytotoxic T lymphocytes with peptides in vitro: identification of candidate T-cell epitopes in hepatitis B virus X antigen / Mi Kyung Chung; Hee Sik Yoon; Sung Shik Min; Hee Gu Lee; Young Jae Kim; Tae Gyu Lee; Jong Soon Lim; Chang Min Kim; Sue Nie Park , 1999 ...
The CD15 antigen (Lewis x or Lex) is the lacto-N-(neo) fucopentaose III molecule. This carbohydrate epitope is carried by both glycolipids and glycoproteins expressed on the cell membrane. CD15 antigen is strongly expressed by neutrophils, eosinophils, monocytes, macrophages, mast cells and normal myeloid precursor cells. It is not expressed on normal erythrocytes, platelets or lymphocytes ...
Feeder layers are used to support the growth of a variety of fastidious cell types including stem cells. Mitotically arrested feeder cells serve as a basal layer for other cultures, supplying important metabolites without further division of their own.
Feeder layers are used to support the growth of a variety of fastidious cell types including stem cells. Mitotically arrested feeder cells serve as a basal layer for other cultures, supplying important metabolites without further division of their own.
LEPOR (LEges POpuli Romani) est une base de données en cours délaboration qui, lorsquelle sera achevée, comprendra environ 880 notices, dont chacune sera consacrée à une loi comitiale du peuple romain. Nous entendons par « lois comitiales » les lois et plébiscites votés par le peuple ou par la plèbe et les rogationes qui ont au moins été promulguées. Sont exclues les « lois royales » et les chartes octroyées à des collectivités (souvent appelées leges datae). Les textes législatifs retenus vont de 509 aux lois attribuées à Nerva, et les plébiscites antérieurs à 287 feront lobjet de notices bien quils naient pas encore eu valeur normative pour lensemble du peuple. Le but est de remplacer louvrage toujours utilisé mais bien vieilli maintenant, de Giovanni Rotondi, Leges publicae populi Romani, Milan, 1912. Il ne sagit pas seulement dune mise à jour, puisque la totalité des sources anciennes et de la bibliographie antérieure ou postérieure à Rotondi ont été ...
PURPOSE: Sialyl Tn (STn) antigen is a cancer-associated carbohydrate antigen expressed in cancers of the digestive tract. We compared the proportion of specimens of flat-type colorectal cancers...
Find and save ideas about falda globo Via Uno 2018 on Pinterest. | See more ideas about Blusa de satén Adolfo Domínguez, Falda recta en malva and Blusa maxi Tendenza.
Although I am a die-hard CrossFitter, I spent some time recently working out at a globo gym. My experience wasnt what I expected at all, and I was pleasantly surprised at what I learned.
Howard Levy, Eugene Friesen and Glen Velez have been performing in the genre-smashing group Trio Globo since the early 1990s. Dont let the name fool you.
CA 19-9, 1.0 ml. CA19-9, a carbohydrate epitope expressed on a high MW (| 400kDa) mucin glycoprotein, is a sialyl Lewis|sup|a|/sup| structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the
You should know that the Lex Bronkowitz Orchestra is a project by Alexander Debus. Alexander, Alex or Lex, has been recording Zappa compositions for quite a number of years and has shared these on CDR. In my book, these recordings qualify as some of the most interesting reworkings / rearrangements of Zappa pieces that I have ever heard ...
... - Title: CIL II(2).5, - Lex Ursonensis, bronze tablets with municipal law on the foundation of Colonia Iulia Genetiva at Urso. Author: Center for
Cara Zwibel is Acting General Counsel at the Canadian Civil Liberties Association. @CaraZwibel. Lex Gill is an Advocate on the National Security Program at Canadian Civil Liberties Association (CCLA). @Lex_is ...
Meet Lex Luther the Shih Tzu on the Daily Puppy. Read about Lex Luther, the Shih Tzu or any other breed of dog. Connect with other dog owners on DailyPuppy.com.
6611890DNAC. griseus 1aacagaaact tattttcctg tgtggctaac tagaaccaga gtacaatgtt tccaattctt 60tgagctccga gaagacagaa gggagttgaa actctgaaaa tgcgggcatg gactggttcc 120tggcgttgga ttatgctcat tctttttgcc tgggggacct tattgtttta tataggtggt 180catttggttc gagataatga ccaccctgac cattctagca gagaactctc caagattctt 240gcaaagctgg agcgcttaaa acaacaaaat gaagacttga ggagaatggc tgagtctctc 300cgaataccag aaggccctat tgatcagggg acagctacag gaagagtccg tgttttagaa 360gaacagcttg ttaaggccaa agaacagatt gaaaattaca agaaacaagc taggaatgat 420ctgggaaagg atcatgaaat cttaaggagg aggattgaaa atggagctaa agagctctgg 480ttttttctac aaagtgaatt gaagaaatta aagaaattag aaggaaacga actccaaaga 540catgcagatg aaattctttt ggatttagga catcatgaaa ggtctatcat gacagatcta 600tactacctca gtcaaacaga tggagcaggt gagtggcggg aaaaagaagc caaagatctg 660acagagctgg tccagcggag aataacatat ctgcagaatc ccaaggactg cagcaaagcc 720agaaagctgg tatgtaatat caacaaaggc tgtggctatg gatgtcaact ccatcatgtg 780gtttactgct tcatgattgc ttatggcacc cagcgaacac tcatcttgga atctcagaat 840tggcgctatg ctactggagg ...
Complete information for FUT6 gene (Protein Coding), Fucosyltransferase 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for FUT6 gene (Protein Coding), Fucosyltransferase 6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
2001 Lex was talking on his cell phone and didnt see the baling wire in the road until it was almost too late. He swerved to the side and found himself heading straight for a tall boy in flannel standing on the side of the bridge. "Ahh!" Lex cried out in surprise and alarm. The boy turned, apparently hearing the sports car heading right for him. His eyes widened in fear and he, too, opened his mouth to shout something. Lex didnt think. He just acted. Hed been part of Dumbledores Army and hed fought in the Battle for Hogwarts during his last year there. The wand was in his hand without any conscious decision to draw it from its arm holster. "Wingardium Leviosa!" he cast, launching the boy up into the air as the car hurtled through the space he had so very recently occupied. He heard no scrapping against the roof, so Lex assumed the boy had been thrown clear. He didnt really have much time to process that, though, because the river came up to met him and when it did, Lexs head slammed ...
A Neo Citran bels leges por feln tteknek s 14 vesn l id sebb serd l knek a megf z s s az influenza t neteinek enyh t s re alkalmazand
Shop SUITE NY for the True Colour Vases by Lex Pott for AndTradition and more modern colorful home accessories in oxidized metal and polished brass
Wrestler Lex Luger (real name: Lawrence Pfohl) was arrested by Cobb County, Georgia cops in May 2003 after they found illegal bodybuilding drugs in his home while investigating the death of his liv
Mike Johnson is reporting that former WCW World champion Lex Lugar is still hospitalized at Stanford University Hospital in Palo Alto, California. Fortunat
Find information for part FLQ.150R from the FLQ Series Midget 10x38mm Fuses, or search for Fuses and more Industrial Power Fuses from Littelfuse.
Marcia Lewis - @ARTISTdirect.com we are all about Latest News & Information on Marcia Lewis Music, Free Marcia Lewis Music Videos, News, Interviews, Fee Music Downloads and More...
One of the main benefits of FUT over FUE is that large numbers of follicular units can be transplanted in one session. The greatest number of follicular units transferred during an FUE treatment is about 2,000. On the other hand, an FUT may involve the transfer of 4,000 follicular units. The FUT method also allows for "super sessions", where up to 5,000 follicular units are transferred in one appointment. For patients who need to transplant a large quantity of follicles, these super sessions provide a great convenience, and are often the most economical option. FUT is also the recommended form of treatment for patients who cannot commit to multiple surgery dates. ...
BANDO Terumi , MUGURUMA Naoki , ITO Susumu , MUSASHI Yoko , INAYAMA Kumi , KUSAKA Yoshihiro , TADATSU Masaya , II Kunio , IRIMURA Tatsuro , SHIBAMURA Seiichi , TAKESAKO Kazuhiro Journal of gastroenterology 37(4), 260-269, 2002-04-01 医中誌Web 参考文献31件 被引用文献3件 ...
Kevin took Lex to see the new eye doctor, who is a Pediatric Opthamologist. Kevin said he was really, really nice, but very serious. They did the eye spray to dilate his pupils, did the various eye exams and told Kevin that Lex is farsighted, but the prescription the other doctor gave us was way off. He said that Lexs right eye is wandering to compensate for the farsightedness. He will need to wear his glasses for 2 weeks straight from the time he gets up till the time he goes to bed. Now, for the good news.......it will correct its self!!!!!! YAY!!!!!!!!!!!!!!!!! I have never been so relieved in my life. Well have to take him back in 3 weeks for a follow up and see how things are then. I am so happy! And Im glad I dont just "take the doctors word for it ...
N2O, or nitrous oxide, has many Lewis dot structures since both nitrogen and oxygen can act as the center atom. In total, there are three valid Lewis dot structures for nitrous oxide....
TRA-1-60 (R)小鼠单克隆抗体[TRA-1-60](ab16288)可与兔, 人样本反应并经WB, IP, RIA, IHC, ICC, Flow Cyt, ICC/IF实验严格验证,被22篇文献引用并得到5个独立的用户反馈。
TRA-1-81小鼠单克隆抗体[TRA-1-81](ab16289)可与兔, 人样本反应并经WB, IP, RIA, IHC, ICC, Flow Cyt, ICC/IF实验严格验证,被15篇文献引用并得到2个独立的用户反馈。
Model Mayhem is the #1 portfolio website for professional models and photographers. Create a profile, upload your photos and connect with other professionals
A leucemia mieloide aguda (LMA) é uma neoplasia que afeta a maturação das células-tronco hematopoiéticas (CTH), comprometendo a produção dos componentes saudáveis da medula óssea. A LMA acomete pessoas...
See what Lewis Gurney (lewisgurney) has discovered on Pinterest, the worlds biggest collection of everybodys favorite things.
Before you buy Sassy Teething Feeder, check out 208 Influenster reviews. Charlene M. said Neat idea. It is definitely a cool idea that you could share...
Aaron Equipment buys, sells, and trades Used- Schenk AccuRate Dry Material Feeder, Model . Submit a quote for this Volumetric or call 630-350-2200 for more information.
Aaron Equipment buys, sells, and trades Used- Acrison Volumetric Dry Ingredient Feeder, M. Submit a quote for this Volumetric or call 630-350-2200 for more information.
Notice: Please do not include any confidential or sensitive information in this form. This form sends information by non-encrypted e-mail which is not secure. Submitting this form does not create an attorney-client relationship.. ...

Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.  - PubMed - NCBITherapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. - PubMed - NCBI

Stem Cells Transl Med. 2015 Feb;4(2):146-55. doi: 10.5966/sctm.2014-0181. Epub 2015 Jan 5. Research Support, Non-U.S. Govt ... Abbreviations: ASCs, adipose tissue-derived stem/stromal cells; SSEA-3, stage-specific embryonic antigen-3. ... Stage-specific embryonic antigen-3 (SSEA-3)-positive multipotent mesenchymal cells (multilineage differentiating stress- ... Adipose; Adult stem cells; Cell culture; Endothelial cell; Hyaluronan; Mesenchymal stem cells; Stem cell transplantation; ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/?term=25561682

Ethical and Safety Issues of Stem Cell-Based TherapyEthical and Safety Issues of Stem Cell-Based Therapy

... stage specific embryonic antigens 3 and 4 (SSEA-3 and SSEA-4), TRA-1-60, and TRA-1-81.These cells are pluripotent, meaning they ... Autologous adipose tissue-derived stem cells for the treatment of Crohns fistula: a phase I clinical study. Cell Transplant. ... mesenchymal stem cells; OCT3/4: octamer-binding transcription factor 3/4; SSEA-3 and SSEA-4: stage specific embryonic antigens ... Keywords: embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, stem cell-based therapy. ...
more infohttp://www.medsci.org/v15p0036.htm

Christchurch Regenerative Medicine and Tissue Engineering research group Publications, Christchurch Regenerative Medicine and...Christchurch Regenerative Medicine and Tissue Engineering research group Publications, Christchurch Regenerative Medicine and...

Published research of the Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) research group, University of ... Schrobback, K., Wrobel, J., Hutmacher, D. W., Woodfield, T. B. F., & Klein, T. J. (2013). Stage-specific embryonic antigen-4 is ... tissue-engineered implants for restoring congruent articulating surfaces in small joints. Cell Proliferation, 42(4), 485-497. ... Schrobback, K., Wrobel, J., Hutmacher, D. W., Woodfield, T. B. F., & Klein, T. J. (2013). Stage-specific embryonic antigen-4 is ...
more infohttps://www.otago.ac.nz/regenerative-medicine/publications/index.html

Restoring the quantity and quality of elderly human mesenchymal stem cells for autologous cell-based therapies.  - PubMed - NCBIRestoring the quantity and quality of elderly human mesenchymal stem cells for autologous cell-based therapies. - PubMed - NCBI

Stem Cell Res Ther. 2017 Oct 27;8(1):239. doi: 10.1186/s13287-017-0688-x. ... ECM extracellular matrix, FSC forward scatter, MSC mesenchymal stem cell, SSEA-4 stage-specific embryonic antigen-4 ... S small, L large, Y young, E elderly, ECM extracellular matrix, TCP tissue culture plastic, ATP adenosine triphosphate, RFU ... The elderly MSC population was isolated into four subpopulations based on size and stage-specific embryonic antigen-4 (SSEA-4) ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/29078802

Christopher Niyibizi, MSc, PhD - Research Output
     - Penn StateChristopher Niyibizi, MSc, PhD - Research Output - Penn State

Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression. Maddox, J ... Multipotent mesenchymal stem cells from human subacromial bursa: Potential for cell based tendon tissue engineering. Song, N., ... Li, F. & Niyibizi, C., Dec 1 2015, In : Cells Tissues Organs. 201, 1, p. 14-25 12 p.. Research output: Contribution to journal ... Cells derived from murine induced pluripotent stem cells (iPSC) by treatment with members of TGF-beta family give rise to ...
more infohttps://pennstate.pure.elsevier.com/en/persons/christopher-niyibizi/publications/?ordering=title&descending=false

Antibody tools to deplete or isolate teratogenic, cardiac, and blood stem cells from hESCs | Californias Stem Cell AgencyAntibody tools to deplete or isolate teratogenic, cardiac, and blood stem cells from hESCs | California's Stem Cell Agency

To this end, we tested an antibody aimed at a pluripotent cell marker (stage-specific embryonic antigen-5 [SSEA-5]) that we ... to become tissue-specific stem cells that exclusively develop into only heart, blood cells, blood vessel lining cells, cells ... cells develop into desired tissue-specific stem cells or to separate these desired cells from a mixture of many types of cells ... tissue-specific stem cells as they exist in the body or as derived from pluripotent cells are the true targets of stem cell- ...
more infohttps://www.cirm.ca.gov/our-progress/awards/antibody-tools-deplete-or-isolate-teratogenic-cardiac-and-blood-stem-cells-hescs

Symposium on Student Research, Creative Activities, & Innovation | Graduate Studies & Research Home | CSUSMSymposium on Student Research, Creative Activities, & Innovation | Graduate Studies & Research Home | CSUSM

Graduate In-ovo Culture of Cryopreserved Quail Testicular Tissue Increases Cell Survivability and Stage-Specific Embryonic ... Antigen -1, -3, and -4 Percentages. *Jesus Perez - Dr. Stephen Tsui, Faculty Mentor Applied Physics, Undergraduate Oxygen- ...
more infohttps://csusm.edu/gsr/student/src.html

Umbilical Cord Tissue-Derived Cells as Therapeutic AgentsUmbilical Cord Tissue-Derived Cells as Therapeutic Agents

... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... "Stage-specific embryonic antigen 4 in whartons jelly-derived mesenchymal stem cells is not a marker for proliferation and ... Development of cell and tissue cryobanking facilities allows the cord tissue as well as UC-derived cells to be cryogenically ...
more infohttps://www.hindawi.com/journals/sci/2015/150609/

Cell Transplantation for Spinal Cord Injury: A Systematic ReviewCell Transplantation for Spinal Cord Injury: A Systematic Review

According to the report from Matsuda et al, the stage-specific embryonic antigen-1 (SSEA-1) expression, which is considered as ... "Markers of pluripotency and differentiation in human neural precursor cells derived from embryonic stem cells and CNS tissue," ... 4. Embryonic Stem Cells and Induced Pluripotent Stem Cells. Embryonic stem cells (ESCs) are a kind of pluripotent stem cells ... "Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations," Stem Cells, vol. 25, no. 9 ...
more infohttps://www.hindawi.com/journals/bmri/2013/786475/

Dental Stem Cells: Sources and Potential Applications | Springer for Research & DevelopmentDental Stem Cells: Sources and Potential Applications | Springer for Research & Development

... stem cell research in dentistry has grown rapidly with the potential application for oral and maxillofacial tissue regeneration ... Mesenchymal stem cells (MSCs) from the oral and... ... stage specific embryonic antigens (SSEA-3, -4), and tumor ... MSCs Mesenchymal Stem Cells DSCs Dental Stem Cells DPSCs Dental Pulp Stem Cells SHED Stem Cells From Human Exfoliated Deciduous ... Stem cells can be divided into three main types, embryonic stem cells (ESCs), adult or postnatal stem cells (ASCs), and induced ...
more infohttps://rd.springer.com/article/10.1007%2Fs40496-014-0012-0

STEM CELL THERAPY FOR BLOOD VESSEL DEGENERATION - Riordan, Neil H.STEM CELL THERAPY FOR BLOOD VESSEL DEGENERATION - Riordan, Neil H.

... provides means of treating degenerated blood vessels through administration of stem cells or activators of stem cells. In one ... embryonic stem cells are totipotent and may express one or more antigens selected from a group consisting of: stage-specific ... placental tissue, peripheral blood mononuclear cells, differentiated embryonic stem cells, and differentiated progenitor cells. ... embryonic stem cells, cord blood stem cells, placental stem cells, bone marrow stem cells, amniotic fluid stem cells, neuronal ...
more infohttp://www.freepatentsonline.com/y2009/0291061.html

JCI -
A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in...JCI - A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in...

Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem ... cells, developed teratomas in the scar tissue, whereas those transplanted with purified SSEA-1+ cells did not. We therefore ... stage-specific embryonic antigen 1 (SSEA-1). BMP2-induced SSEA-1+ cells were purified from ES and iPS cells and could be ... MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. Cell Stem Cell. 2008;2(3):219-229.. View this ...
more infohttps://www.jci.org/articles/view/40120

120(4)120(4)

... stage-specific embryonic antigen 1 (SSEA-1). BMP2-induced SSEA-1+ cells were purified from ES and iPS cells and could be ... cells, developed teratomas in the scar tissue, whereas those transplanted with purified SSEA-1+ cells did not. We therefore ... stage-specific embryonic antigen 1 (SSEA-1), and mesoderm posterior 1 (MESP1), derived from human pluripotent stem cells ... When combined with chemotherapy, CTLs raised against specific antigens were able to induce apoptosis in neighboring tumor cells ...
more infohttps://www.jci.org/120/4

Modulation of Hematopoietic Stem Cell Differentiation - ICHIM CHRISTINE VICTORIAModulation of Hematopoietic Stem Cell Differentiation - ICHIM CHRISTINE VICTORIA

The invention provides means of manipulating hematopoietic stem cell differentiation by modulation of levels of NR2F6 (EAR2). ... said embryonic stem cells are totipotent and express one or more antigens selected from a group consisting of: stage-specific ... dermis tissue, subcutaneous tissue, heart tissue, lung tissue, vascular tissue, endothelial tissue, blood cells, bladder tissue ... dermis tissue, subcutaneous tissue, heart tissue, lung tissue, vascular tissue, endothelial tissue, blood cells, bladder tissue ...
more infohttp://www.freepatentsonline.com/y2015/0299712.html

3D Cell Printed Tissue Analogues: A New Platform for Theranostics3D Cell Printed Tissue Analogues: A New Platform for Theranostics

... such as stage-specific embryonic antigen 1, octamer-binding transcription factor 4, and Nanog. ... Moreover, if autologous cells or patient-derived induced pluripotent stem cells are used, patient-specific tissue or ... Since human organs and tissues are composed of various cell types in specific locations, tissues should be engineered to ... 3D cell printed living tissue in regenerative medicine. 3D cell printing of living tissues for regeneration and repair. In this ...
more infohttp://thno.org/v07p3118.htm

Poly(ADP-ribose) polymerase 1 regulates nuclear reprogramming and promotes iPSC generation without c-Myc | JEMPoly(ADP-ribose) polymerase 1 regulates nuclear reprogramming and promotes iPSC generation without c-Myc | JEM

3 E), stage-specific embryonic antigen (SSEA-1) and Oct4, and protein of stemness factors (Fig. 3 F). Bisulfite sequencing ... Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 1:55-70 ... Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell. 5:214-226. doi:10.1016/j. ... Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells. Stem Cells. 22:962- ...
more infohttp://jem.rupress.org/content/210/1/85

Appendix C: Human Embryonic Stem Cells and Human Embryonic Germ Cells | stemcells.nih.govAppendix C: Human Embryonic Stem Cells and Human Embryonic Germ Cells | stemcells.nih.gov

Embryonic stem cell. EG cell = Embryonic germ cell. EC cell = Embryonal carcinoma cell. SSEA = Stage-specific embryonic antigen ... Telomerase activity and long telomeres are characteristic of proliferating cells in embryonic tissues and of germ cells. Human ... Like monkey ES cells [34], human ES cells express a panel of surface makers that include the stage-specific embryonic antigens ... Appendix C: Human Embryonic Stem Cells and Human Embryonic Germ Cells. Methods for Growing Human Embryonic Stem Cells In Vitro ...
more infohttps://stemcells.nih.gov/info/2001report/appendixC.htm

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Antigens can be induced in transgenic mice dei cient for condensation molecular basis of embryonic xenopus cells to increased ... tissues and structures in the bmp gene family of growth and involution seen in front of an e mouse embryos at specific stages ... They may not be the result of protein into the peritoneal dialysis as an example is wt an early role in cella cell junctions ... m r rao m h pointu h roullot v pignot paintrandvernet m and sariola h neuronal characteristics in embryonic and adult tissues ...
more infohttp://www.nationalnewstoday.com/medical/where-to-buy-viagra-in-chicago/2/

Creation of an in vitro microenvironment to enhance human fetal synovium-derived stem cell chondrogenesis | SpringerLinkCreation of an in vitro microenvironment to enhance human fetal synovium-derived stem cell chondrogenesis | SpringerLink

... and low oxygen to enhance chondrogenesis in human fetal synovium-derived stem cells (hfSDSCs). Human... ... exhibit a lower expression of senescence-associated β-galactosidase and an enhanced level of stage-specific embryonic antigen-4 ... Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue ... requirement to lock mesenchymal stem cells in a desired differentiation stage. J Cell Physiol 219:219-226PubMedCrossRefGoogle ...
more infohttps://link.springer.com/article/10.1007%2Fs00441-011-1212-8

KAKEN - Research Projects | 2005  Fiscal Year Final Research Report Summary (KAKENHI-PROJECT-14360191)KAKEN - Research Projects | 2005 Fiscal Year Final Research Report Summary (KAKENHI-PROJECT-14360191)

... expressed specific markers such as alkaline phosphatase activity, stage specific embryonic antigen-1 and Oct-4, and formed ... When the simple Ms were cultured in tissue culture plates, they differentiated into several types of cells including neuron- ... embryonic fibroblast cell / estrogen / estrus induction. Research Abstract. Embryonic stem (ES) cells are pluripotent cells ... and co-culture with canine embryonic fibroblasts developed the embryo up to the 16-cell stage, and with mouse embryonic ...
more infohttps://kaken.nii.ac.jp/en/report/KAKENHI-PROJECT-14360191/143601912005kenkyu_seika_hokoku_gaiyo/

Spirituality | Futurist  Transhuman  News  Blog | Page 3Spirituality | Futurist Transhuman News Blog | Page 3

The cell surface antigens most commonly used to identify hES cells are the glycolipids stage specific embryonic antigen 3 and 4 ... Under normal circumstances these tissue stem cells, or adult stem cells, will generate the type of cells that make up the organ ... Specialized cells like bone cells, liver cells, and heart cells begin as stem cells. The process of the cells transforming from ... Embryonic stem cells (ESCs) are the cells of the inner cell mass of a blastocyst, an early-stage embryo.[12] Human embryos ...
more infohttps://www.euvolution.com/futurist-transhuman-news-blog/category/spirituality/page/3

Stem cell - Wikipedia | Futurist  Transhuman  News  BlogStem cell - Wikipedia | Futurist Transhuman News Blog

The cell surface antigens most commonly used to identify hES cells are the glycolipids stage specific embryonic antigen 3 and 4 ... into specialized cell types with characteristics consistent with cells of various tissues such as muscles or nerves. Embryonic ... Embryonic stem cells (ESCs) are the cells of the inner cell mass of a blastocyst, an early-stage embryo.[12] Human embryos ... By using human embryonic stem cells to produce specialized cells like nerve cells or heart cells in the lab, scientists can ...
more infohttps://www.euvolution.com/futurist-transhuman-news-blog/liberty/stem-cell-wikipedia-37.php

Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix | Scientific...Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix | Scientific...

... both of which are involved in the regulation of endothelial cell adhesion and migration. For fibronectin, the major proteolytic ... leading to an increase in cell migration. Furthermore, a polyclonal antibody raised against KLK12′s proteolytic cleavage site ... migration and stabilization of endothelial cells requires extracellular matrix remodeling. To characterize the molecular ... the KLK12-dependent inhibition of fibronectin polymerization and the KLK12-mediated pro-migratory effect on endothelial cells. ...
more infohttps://www.nature.com/articles/s41598-018-24576-9?error=cookies_not_supported&code=ff98cf9f-98b3-4ccc-9481-677dfae716f9

Notch Signaling: Cell Fate Control and Signal Integration in Development | ScienceNotch Signaling: Cell Fate Control and Signal Integration in Development | Science

Although the core elements may be ubiquitous, cell- or tissue-specific factors are likely to exist. Given the multitude of ... With antibodies against specific antigens as well as enhancer- and promoter-trap lacZ lines, which permit the labeling of most ... embryonic tissues, it was demonstrated that a loss of Notch signaling results in abnormalities in tissues derived from all ... For instance, Notch appears to be involved in most, if not all, stages of eye development. Early in development, the expression ...
more infohttps://science.sciencemag.org/content/284/5415/770?ijkey=51fe6d446b63a9f350ae6f7ce5156ab0bd5a080b&keytype2=tf_ipsecsha

Safer stem cell therapy - NextBigFuture.comSafer stem cell therapy - NextBigFuture.com

... designated anti-stage-specific embryonic antigen (SSEA)-5, which binds a previously unidentified antigen highly and ... Immunohistochemistry studies of human fetal tissues and bioinformatics analysis of a microarray database revealed that ... An important risk in the clinical application of human pluripotent stem cells (hPSCs), including human embryonic and induced ... Then they used FACS to pick out cells the antibody bound to. Finally, they injected the remaining cells into mice to see if ...
more infohttps://www.nextbigfuture.com/2011/08/safer-stem-cell-therapy.html
  • When injected into immune-deficient mice, snow leopard iPS cells formed teratomas containing tissues representative of the three germ layers. (blogspot.com)
  • They are able to generate cell lines derived from any of the three embryonic germ layers (ectoderm, mesoderm and endoderm), giving them great therapeutic potential. (intechopen.com)
  • Synthetic OCT4 mRNA was recently used (in conjunction with other reprogramming factors) to generate human induced pluripotent stem cells. (biomedcentral.com)
  • Here, we discovered that BAY 11-7082 (BAY11), at least partially through an NF-κB-inhibition based mechanism, could significantly increase the expression of OCT4 following transfection of synthetic mRNA (synRNA) into adult human skin cells. (biomedcentral.com)
  • We found that human skin cells cultured in the presence of BAY11 resulted in reproducible increased expression of OCT4 that did not inhibit normal cell proliferation. (biomedcentral.com)
  • Thus, the delivery and stable expression of synthetic OCT4 mRNA and other synthetic mRNAs (synRNAs) may have multiple applications for future cell-based research and therapeutics. (biomedcentral.com)
  • RT-PCR also confirmed that endogenous OCT4 and NANOG were expressed by snow leopard iPS cells from P4. (blogspot.com)
  • The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. (wikipedia.org)
  • The aim of the present study was to investigate the effect of specific culture conditions on the differentiation of hESC into lung epithelial cells. (biomedcentral.com)
  • These results suggest that by using our novel culture protocol hESC can be differentiated into the major cell types of lung epithelial tissue. (biomedcentral.com)
  • The respiratory system originates from the foregut endoderm, differentiating into many kinds of specialized epithelial cells. (biomedcentral.com)
  • Among epithelial cells of the ovarian surface epithelium in women with serous ovarian carcinoma we observed a population of small NANOG-positive cells with diameters of up to 5 μm and nuclei, which filled almost the entire cell volumes. (biomedcentral.com)
  • These small NANOG-positive cells were in some cases concentrated in the regions with morphologically changed epithelial cells. (biomedcentral.com)
  • These cells are proposed as CSCs, and possibly originate from small stem cells among epithelial cells. (biomedcentral.com)
  • Conditional knockout studies in mouse liver and mammalian epithelial tissue have confirmed the importance of MED1/TRAP220 in several NR signaling pathways in vivo ( 15 - 17 ). (aacrjournals.org)
  • E-cadherin, a calcium dependent cell adhesion molecule, is expressed predominately in epithelial tissues and plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. (acris-antibodies.com)
  • Microchannels in development, survival, and vascularisation of tissue analogues for regenerative medicine. (otago.ac.nz)
  • Although clinical application of mesenchymal stem cells (MSCs) has shown beneficial effects in the therapy of autoimmune and chronic inflammatory diseases, the ability to promote tumor growth and metastasis and overestimated therapeutic potential of MSCs still provide concerns for the field of regenerative medicine. (medsci.org)
  • Stem cell researchers are engaged in different endeavors, including treating genetic disorders and generating new stem cell-derived human tissues and biomaterials for use in pharmacy genomics and regenerative medicine. (medsci.org)
  • There is a plethora of papers covering the topic of stem cells (SC) and their potential for use in regenerative medicine [ 1 - 5 ]. (hindawi.com)
  • This cell population is mentioned as a source of cell material for usage in various fields of regenerative medicine [ 18 , 19 ]. (hindawi.com)
  • 27 ] describes in detail the main properties of UC-derived cells that allow them to be used in regenerative medicine. (hindawi.com)
  • Three-dimensional (3D) cell printing is an emerging technology in the field of regenerative medicine. (thno.org)
  • The ability to do regenerative medicine requires the complete removal of tumor-forming cells from any culture that began with pluripotent cells," said Irving Weissman, MD, director of the Stanford Institute for Stem Cell Biology and Regenerative Medicine. (bio-medicine.org)
  • Weissman pointed out that the production of therapeutic cells from pluripotent stem cells for regenerative medicine was a major goal of Proposition 71, the ballot measure that established the California Institute for Regenerative Medicine to allocate $3 billion to advance stem cell science. (bio-medicine.org)
  • The therapeutic use of stem cells to treat diseases and injuries is a promising tool in regenerative medicine. (therapeuticsolutionsint.com)
  • The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine. (patentsencyclopedia.com)
  • An object of the present invention is to provide a novel medical application to regenerative medicine that uses pluripotent stem cells (Muse cells). (patentsencyclopedia.com)
  • The present invention relates to a cell preparation used in regenerative medicine. (patentsencyclopedia.com)
  • In regenerative medicine, to identify a source of stem cells of high safety and efficacy is the first step of the development of bio materials for repairing and renewing damaged and defective tissues. (justia.com)
  • Degenerative diseases are a major public health concern for the aging population and mesenchymal stem cells (MSCs) have great potential for treating many of these diseases. (nih.gov)
  • The original populations (young and elderly MSCs), as well as the four subpopulations, were then characterized before and after culture on tissue culture plastic and BM-derived extracellular matrix (BM-ECM). (nih.gov)
  • These results suggest that it is feasible to obtain large numbers of high-quality autologous MSCs from the elderly population and establish personal stem cell banks that will allow serial infusions of "rejuvenated" MSCs for treating age-related diseases. (nih.gov)
  • However, it has been shown in a number of works that these cell populations exhibit broader "stem features" than MSCs from adult sources [ 25 , 26 ]. (hindawi.com)
  • In this review, we provide an overview of different types of MSCs that have been isolated and characterized from several origins such as dental pulp, exfoliated deciduous teeth, the periodontal ligament, the dental follicle, the dental papilla, oral mucosa, and gingiva, with the focus on the potential clinical applications for each type of dental stem cell. (springer.com)
  • Pre- and postnatal ULSCs are morphologically similar to mesenchymal stem cells (MSCs) and easily expand to greater than 70 population doublings. (therapeuticsolutionsint.com)
  • Mesenchymal stem cells (MSCs) are a valuable source of cells that have the capability of self-renewal and multi lineage potential and have shown promising clinical results in safety and efficacy studies for treating cardio vascular and orthopedic disorders ( 14 , 15 , 21 ). (therapeuticsolutionsint.com)
  • The most commonly utilized cell lineages were covered in this paper and specific areas covered include survival of grafted cells, axonal regeneration and remyelination, sensory and motor functional recovery, and electrophysiological improvements. (hindawi.com)
  • In recent years, stem cell research in dentistry has grown rapidly with the potential application for oral and maxillofacial tissue regeneration. (springer.com)
  • The objective of this review is to describe new findings in the field of dental stem cell research and their potential applications in tissue regeneration and therapy of inflammation-related diseases. (springer.com)
  • hfSDSCs might represent a promising cell source for cartilage tissue engineering and regeneration. (springer.com)
  • Because ASCs are responsible for repair and regeneration of injured tissues, and because injured ACLs and MCLs have differential healing capacities as noted above, we hypothesized in this study that both human ACLs and MCLs contain ASCs, but that they exhibit distinctive, ligament-specific properties. (biomedcentral.com)
  • EMT, a physiological cell process during embryonic development and later in life during regeneration, could, when induced in pathological condition, generate CSCs-like cells. (biomedcentral.com)
  • Although the characteristics of SC, including UC-derived cells, are a dramatically discussed issue, this review will focus particularly on some controversial issues regarding clinical utility of cells isolated from UC tissue. (hindawi.com)
  • The impact of UC topography on cell characteristics is briefly discussed. (hindawi.com)
  • Several excellent reviews focused on the characteristics of UC cells and clinical research are currently available. (hindawi.com)
  • Self-renewal and multipotential differentiation are the two main characteristics of stem cells, and embryonic stem cells and adult stem cells are the two major categories [ 4 , 5 ]. (hindawi.com)
  • Stem cells can now be artificially grown and transformed (differentiated) into specialized cell types with characteristics consistent with cells of various tissues such as muscles or nerves. (euvolution.com)
  • The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. (google.com)
  • In standard anatomical pathology, a diagnosis is made on the basis of cell morphology and staining characteristics. (google.com)
  • Transmission electron micrograph of an adult stem cell displaying typical ultrastructural characteristics. (wikipedia.org)
  • So say pleiotropic effects of oncogene, which defines the entire complex of characteristics of tumor cells. (medicalency.com)
  • the cells could be expanded for greater than 90 population doubling and had mesenchymal stem cell characteristics, expressing CD9, SSEA4, CD44, CD90, CD166, CD73, and CD146 but were negative for STRO-1. (therapeuticsolutionsint.com)
  • The action of the oncogene is beyond the scope of natural regulation, proteins begin to operate abnormally, and contribute to the transformation of normal cells in the tumor. (medicalency.com)
  • The outer membrane of cancer cells can be depleted antigens compatibility, but it contains proteins early stages of development (embryonic) or tumor-specific molecules with different immunological properties. (medicalency.com)
  • Because AR activity in prostate cells is critically dependent on interactions with an assortment of coregulatory proteins and complexes ( 4 ), it has been proposed that overexpression or hyperactivation of distinct AR cofactors may facilitate important oncogenic events in both prostate cancer initiation and progression to the androgen-independent disease ( 3 , 4 ). (aacrjournals.org)
  • Consistent with this notion, MED1/TRAP220 can serve as a binding target for other transactivators involved in cell growth and development including the GATA family proteins ( 22 ), p53 ( 23 ), BRCA-1 ( 24 ), and GABPα ( 25 ). (aacrjournals.org)
  • At the nonpermissive temperature, cessation of large T antigen expression was accompanied by induction of p53, as well as the p53-dependent proteins, wild-type p53-activated fragment-1/Cdk (cyclin-dependent kinase)-interacting protein-1 (p21/Waf1), Bcl (B-cell lymphoma)-associated protein (Bax), and murine double minute 2 (MDM2), that lead to cell cycle-arrest, suicide, and p53 inhibition, respectively. (jneurosci.org)
  • Sox proteins are involved in different developmental processes like embryonic development and determination of cell fate. (acris-antibodies.com)
  • However, addition of NANOG , to the transfection cocktail produced stable iPS cell colonies, which formed as early as D3. (blogspot.com)
  • Fig. 5: Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with Nanog antibody Cat. (acris-antibodies.com)
  • Human somatic cells, however, do not show telomerase activity and their telomeres are considerably shorter. (nih.gov)
  • Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells. (google.es)
  • Results obtained from completed and on-going clinical studies indicate huge therapeutic potential of stem cell-based therapy in the treatment of degenerative, autoimmune and genetic disorders. (medsci.org)
  • We describe and discuss ethical challenges regarding human embryonic stem cell (hESC) research, therapeutic potential and clinical translation of induced pluripotent stem cell (iPSC) and safety issues of mesenchymal stem cell (MSC)-based therapy. (medsci.org)
  • Stem cell theranostics has received much attention for noninvasively monitoring and tracing transplanted therapeutic stem cells through imaging agents and imaging modalities. (thno.org)
  • This chapter highlights the current research with UCB cells in the development of a novel treatment for stroke and demonstrates the great therapeutic potential of these cells. (springer.com)
  • Weissman pointed out that the production of therapeutic cells from plu. (bio-medicine.org)
  • With the right combination of growth factors, skill and patience, a laboratory tissue culture dish promises to yield therapeutic wonders. (bio-medicine.org)
  • We've used a combination of antibodies to weed out the few undifferentiated cells that could be left in the 10 or 100 million differentiated cells that make up a therapeutic dose. (bio-medicine.org)
  • Taking into account the advances in the isolation of new subpopulation of stem cells and in the creation of artificial stem cell niches, we discuss how these emerging technologies offer great promises for therapeutic approaches to muscle diseases and muscle wasting associated with aging. (frontiersin.org)
  • On December 10, 2018 Therapeutic Solutions International, Inc., announced the signing of an agreement between TSOI and Jadi Cell LLC for licensing of the Jadi Cell universal donor adult stem cell, as covered in US Patent No.: 9,803,176 B2 for use in Chronic Traumatic Encephalopathy (CTE), and Traumatic Brain Injury (TBI). (therapeuticsolutionsint.com)
  • The Jadi Cell product, which belongs to the mesenchymal stem cell (MSC) family of cells, is a unique adult stem cell, which produces higher levels of therapeutic factors compared to other stem cells. (therapeuticsolutionsint.com)
  • These studies demonstrate the therapeutic potential of an otherwise discarded tissue. (therapeuticsolutionsint.com)
  • therefore, they may serve as a therapeutic cell source. (therapeuticsolutionsint.com)
  • The article focuses on the therapeutic effect of stem cells in treating a wide variety of medical conditions. (ebscohost.com)
  • This result suggests that the target of AHCC in expressing therapeutic efficacy was not the pluripotent cells such as cancer stem cells (CSCs) but SOX2-specific. (cancerindex.org)
  • Sex-determining region Y-box 2 (SOX2) is reported to be up-regulated in other kinds of cancer cells and involved in carcinogenesis and malignancy. (cancerindex.org)
  • The aim of this study was to investigate the effects of AHCC on protein expression of SOX2 in the gemcitabine-resistant pancreatic cancer cell line KLM1-R. (cancerindex.org)
  • AHCC was applied to KLM1-R cells and expression of SOX2 was analyzed by western blotting. (cancerindex.org)
  • AHCC down-regulated SOX2 in KLM1-R cells. (cancerindex.org)
  • Fig. 2: Formalin-fixed and paraffin-embedded ( FFPE ) human lung carcinoma tissue reacted with Sox2 antibody (N-term) Cat. (acris-antibodies.com)
  • The positive and negative cell fractions after MACS separation were used as Muse-rich and Muse-poor cell populations, respectively, in subsequent experiments. (nih.gov)
  • Stem cell therapy for SCI is based on a strategy to treat the injuries and to restore lost functions by replacing lost or damaged cell populations [ 3 ]. (hindawi.com)
  • There is considerable debate as to whether some proposed adult cell populations are truly stem cells. (euvolution.com)
  • The scientists believe the technique could also be used to remove residual tumor-initiating cells from populations of cells derived from induced pluripotent stem, or iPS, cells. (bio-medicine.org)
  • This invention provides populations human cells of the cardiomyocyte lineage. (google.com)
  • Understanding the mechanisms and the extrinsic regulation of cell-fate decisions in stem cell populations and the identification and isolation of pure cell lineages are two major goals of stem biology. (spiedigitallibrary.org)