Basic functional unit of plants.
A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).
Genus of coniferous yew trees or shrubs, several species of which have medicinal uses. Notable is the Pacific yew, Taxus brevifolia, which is used to make the anti-neoplastic drug taxol (PACLITAXEL).
A plant genus of the family APIACEAE used for flavoring food.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
The process of finding chemicals for potential therapeutic use.
Methods for maintaining or growing CELLS in vitro.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Established cell cultures that have the potential to propagate indefinitely.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A cell line derived from cultured tumor cells.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)
The minute vessels that connect the arterioles and venules.
The initial culturing of cells derived directly from fresh TISSUES.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
Educational institutions for individuals specializing in the field of medicine.
Infection with CHLAMYDOPHILA PSITTACI (formerly Chlamydia psittaci), transmitted to humans by inhalation of dust-borne contaminated nasal secretions or excreta of infected BIRDS. This infection results in a febrile illness characterized by PNEUMONITIS and systemic manifestations.
The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.
Degenerative changes in the RETINA usually of older adults which results in a loss of vision in the center of the visual field (the MACULA LUTEA) because of damage to the retina. It occurs in dry and wet forms.
The single layer of pigment-containing epithelial cells in the RETINA, situated closely to the tips (outer segments) of the RETINAL PHOTORECEPTOR CELLS. These epithelial cells are macroglia that perform essential functions for the photoreceptor cells, such as in nutrient transport, phagocytosis of the shed photoreceptor membranes, and ensuring retinal attachment.
Tumors or cancer of the EYE.
Application of tests and examinations to identify visual defects or vision disorders occurring in specific populations, as in school children, the elderly, etc. It is differentiated from VISION TESTS, which are given to evaluate/measure individual visual performance not related to a specific population.
Defects of color vision are mainly hereditary traits but can be secondary to acquired or developmental abnormalities in the CONES (RETINA). Severity of hereditary defects of color vision depends on the degree of mutation of the ROD OPSINS genes (on X CHROMOSOME and CHROMOSOME 3) that code the photopigments for red, green and blue.
Vitreoretinal membrane shrinkage or contraction secondary to the proliferation of primarily retinal pigment epithelial cells and glial cells, particularly fibrous astrocytes, followed by membrane formation. The formation of fibrillar collagen and cellular proliferation appear to be the basis for the contractile properties of the epiretinal and vitreous membranes.

The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)

The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen.  (+info)

In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)

Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (6/155046)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

TY - JOUR. T1 - Lipopolysaccharide extends the lifespan of mouse primary-cultured microglia. AU - Kaneko, Yoko S.. AU - Nakashima, Akira. AU - Mori, Keiji. AU - Nagatsu, Toshiharu. AU - Nagatsu, Ikuko. AU - Ota, Akira. PY - 2009/7/7. Y1 - 2009/7/7. N2 - Microglial activation has been implicated in the recognition and phagocytic removal of degenerating neurons; however, this process must be tightly regulated in the central nervous system, because prolonged activation could damage normal neurons. We report that mouse primary-cultured microglia, which are destined to die within a few days under ordinary culture conditions, can live for more than 1 month when kept activated by lipopolysaccharide (LPS) treatment. Primary-cultured microglia treated with sublethal doses of LPS remained viable, without any measurable increase in apoptotic or necrotic cell death. LPS-treated microglia had an arborescent shape, with enlarged somata and thickened cell bodies. Although the amount of intracellular ATP in ...
Langford test 1 (from quizlet) - infectious diseases 4180 with at muscles. Human Muscle Cell Can Perform Quizlet pleasant in order to the web site, within this moment I will explain to you with regards to Human muscle cell can perform quizlet.. And from now on, this can be the very first picture, human muscle cells can perform quizlet :. ...
The present study was undertaken to elucidate different requirements for CD2-mediated activation of naive (CD45RO-) and memory (CD45RO+) CD4+ T cells. A mitogenic combination of anti-CD2 (anti-T11(2) and anti-T11(3] mAbs could effectively induce the proliferation of memory CD4+ T cells even in the absence of monocytes. In marked contrast, naive CD4+ T cells did not disclose any proliferative responses to anti-CD2 mAbs, when monocytes were absent in culture. This differential responsiveness of naive and memory CD4+ T cells appeared to be related largely to a difference in IL-6-producing ability between both populations. IL-6 among monocyte-derived cytokines could correct unresponsiveness of naive CD4+ T cells to anti-CD2 stimulation. Unlike naive CD4+ T cells, memory CD4+ T cells produced IL-6 by themselves, with its mRNA being expressed on anti-CD2 stimulation. Anti-IL-6R mAb significantly inhibited proliferation of memory CD4+ T cells seen in the anti-CD2-stimulated cultures without monocytes, ...
Differential responsiveness of naive and activated T cells to TL1A that affect Th17 generation, maintenance and proliferation [Abstract]. Immunology 131 (s1) , p. 102. 10.1111/j.1365-2567.2010.03390.x ...
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Microbiology culture preparation. Person using a bunsen flame to sterilise the top of a jar containing a bacterial culture. - Stock Image C024/5723
We offer 4 types of normal human primary cells in the adipose cell system including endothelial cells, preadipocytes and mesenchymal stem cells.
Author: Sözen, Cécile et al.; Genre: Journal Article; Published in Print: 2020-06; Title: Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics
Growth Requirements. The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. To obtain best growth, addition of a small amount of blood serum is usually necessary, and several antibiotics, like penicillin and streptomycin are added to prevent bacterial contamination.. Temperature varies on the type of host cell. Most mammalian cells are maintained at 37oC for optimal growth, while cells derived from cold- blooded animals tolerate a wider temperature range (i.e. 15oC to 26oC). Actively growing cells of log phage should be used which divide rapidly during culture.. Process to obtain primary cell culture. Primary cell cultures are prepared from fresh tissues. Pieces of tissues from the organ are removed aseptically; which are ...
Human Prostate Microvascular Endothelial Cell Pellet ...
TY - JOUR. T1 - Transforming growth factor-β in GI neoplasia, wound healing and immune response. AU - Pignatelli, Massimo. AU - Gilligan, Christopher J.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - The last decade has been marked by tremendous advances in the biochemical and functional characterization of TGF-βs and their receptors in normal and transformed cells. TGF-βs have been shown to modulate proliferation, differentiation and motility of different cell types in a number of in vitro model systems and in some cases with some intriguing results. It is obvious that there is no simple pattern that explains the TGF-βs biological activity in vitro and their effects on cell behaviour need to be assessed in the context of an appropriate physiological cellular environment. Cell-cell and cell-matrix interactions, the differentiating status of the cell together with the functional activity of other soluble growth factors can influence how TGF-βs modulate cell behaviour. However, the overwhelming interest ...
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
Human Esophageal Fibroblast Cell Pellet ...
In 1994, Cell Applications, Inc. recognized the demand for primary cells and set out to galvanize this paradigm shift from cell lines to primary cells. With years of experience handling numerous notoriously finicky primary cell types, we have honed the craft of isolation, purification, subculture, and growth of primary cells. Our company is built on high purity, strictly quality controlled and characterized primary cells, including over 80 different human and animal primary cell types, as well as cell growth media and cell culture reagents, cell marker and signaling antibodies. These comprise a continuously growing cell culture toolbox. ...
CellSeed, in Japan, is developing a series of cell therapies based on its regenerated human cell culture technology (UpCell®) for the potential treatment of
PromoCells facility remains operational and we continue to support researchers worldwide. We are proud of making a contribution to the fight against SARS-CoV-2 with our products. We are here to help - contact us. Dismiss. ...
Given the fact that IL-10 is especially produced by immune cells, we investigated whether the same is true for the other members of the IL-10 family, IL-19, IL-20, IL-22, mda-7, and AK155, and, if so, to what extent. Monocytes, NK cells, B cells, and T cells from the blood of different healthy donors were investigated for their possible constitutive, as well as activation-induced, expression of these molecules. For this purpose, cells were cultured in a kinetic approach in the absence and presence of the typical cell-specific stimuli LPS (monocytes), IL-2/IL-12 (NK cells), fixed S. aureus (B cells), and anti-CD3 mAb coated on the culture vessel (T cells), respectively, for 2, 6, and 18 h. The activation state of control and stimulated cells was verified by the absence and presence of high TNF-α production in the monocyte culture and elevated expression of different activation markers, e.g., CD69, on the lymphocyte populations (Fig. 1⇑A). Gene expression of the IL-10 family members was ...
Ongoing advances in quantitative molecular- and cellular-biology highlight the need for correspondingly quantitative methods in tissue-biology, in which the presence and activity of specific cell-subpopulations can be assessed in situ. However, many experimental techniques disturb the natural tissue balance, making it difficult to draw realistic conclusions concerning in situ cell behaviour. In this study, we present a widely applicable and minimally invasive method which combines fluorescence cell labelling, retrospective image analysis and mathematical data processing to detect the presence and activity of cell subpopulations, using adhesion patterns in STRO-1 immunoselected human mesenchymal populations and the homogeneous osteoblast-like MG63 continuous cell line as an illustration.. Adhesion is considered on tissue culture plastic and fibronectin surfaces, using cell area as a readily obtainable and individual cell specific measure of spreading. The underlying statistical distributions of ...
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
We are the worlds largest primary cell provider, with a variety of normal human and animal primary cells frozen at early passage, cell culture media and reagents, growth factors, cell-derived molecular biology products, cell-based assay kits, stem cell products and gene analysis tools.
The Knowledge Gateway is our open source access to resources. Here is where we share our accumulated resources with this request: Please use them freely yourself, referencing their source, and let us know how you used them and any improvements you made. ...
The PromoCell immuno-oncology portfolio contains characterised human primary cells, including vascular and microvascular endothelial cells, mononuclear cells, macrophages, monocytes and blood progenitor cells - plus optimized cell culture growth medium for each cell type.
Our ethically sourced human primary cell portfolio includes fresh and cryopreserved products from peripheral blood and cord blood.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
8. This allows further bacterial multi- plication topama subsequent production of an increased amount of inflammatory mediators via the complement system and other pathways.
A self-contained mini-greenhouse useful for propagating cuttings, germinating seeds, and acclimating tissue-cultured plants intended for transfer to soil. Includes plastic tray …
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Study Flashcards On PA 2008- Patho ch. 2 at Quickly memorize the terms, phrases and much more. makes it easy to get the grade you want!
Study Flashcards On patho at Quickly memorize the terms, phrases and much more. makes it easy to get the grade you want!
The human system, critical infrastructure, environment, human society, human etc. are represented by system of systems, i.e. they consist...
Cell culture media and reagents for the stimulation, expansion, and differentiation of human and mouse primary cells, including immune cells, stem cells, and neural cells. - Deutschland
هدف: سلول‌های بنیادی پرتوان مشتق شده از بافت بیضه، یک منبع بسیار غنی و جدیدی برای سلول درمانی در پزشکی بازساختی هستند. مطالعات اخیر نشانگر آن است که سلول‌های بنیادی اسپرماتوگونی در شرایط آزمایشگاهی می‌توانند به سلول‌های بنیادی شبه جنینی تغییر یابند.استفاده از امواج فراصوت با شدت پایین، آثار مثبتی روی رشد و تمایز سلول‌ها دارد. هدف از این مطالعه تأثیر امواج فراصوت روی کلونی‌زایی سلول‌های بنیادی شبه جنینی است. مواد و روش‌ها: ابتدا، سلول‌های بنیادی اسپرماتوگونی از بیضه نوزاد موش جدا شدند. بعد از کشت دادن این سلول‌ها در محیط DMEM/F12، حاوی 15 درصد سرم جنین
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The purpose of the present investigation was to explore the effects of well-defined flow conditions on the activity of tissue factor (TF) expressed on the surface of cultured rat vascular smooth muscle cells. Cells were cultured to confluence on Permanox brand slides and stimulated to express TF by a 90 min incubation with fresh growth medium containing 10 percent calf serum. The stimulated cells were then placed in a parallel plate flow chamber and perfused with Hanks Balanced Salt Solution containing factor VIIa, factor X (FX), and calcium. The chamber effluent was collected and assayed for factor Xa (FXa) and the steady-state flux of FXa was calculated. The flux values were 68.73, 94.81, 139.75, 138.19, 316.82, and 592.92 fmole/min/cm2 at wall shear rates of 10, 20, 40, 80, 320, and 1280 s−1 respectively. The FXa flux depended on the wall shear rate to a greater degree than predicted by classical mass transport theory. The flux at each shear rate was three to five times less than that ...
TY - JOUR. T1 - Modulation of collagen synthesis by tumor necrosis factor alpha in cultured vascular smooth muscle cells. AU - Hiraga, Syouichi. AU - Kaji, Toshiyuki. AU - Ueda, Yoshimichi. AU - Zisaki, Fumiko. AU - Iwata, Kazushi. AU - Koizumi, Fumitomo. AU - Okada, Yasunori. AU - Katsuda, Shogo. AU - Nakanishi, Isao. PY - 1999/12/10. Y1 - 1999/12/10. N2 - Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. The synthesis of collagenase-digestible proteins (CDP) and noncollagenous proteins (NCP) was evaluated by the [3H]proline incorporation. It was shown that TNF-α markedly suppresses the incorporation of [3H]proline into both CDP and NCP in confluent cultures of SMCs but not in sparse cultures of the cells. Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. In confluent SMCs, the synthesis of CDP was more ...
I am planning to set up a co-culture system for human endothelial cells and human VASCULAR smooth muscle cells. I would be really interested to find out about methods of extraction and primary culture of human vascular smooth muscle cells, if anyone can help and advise, please mail me! Thanks Pippa Deex ...
Vascular smooth muscle (VSM) cell proliferation contributes to the pathogenesis of atherosclerosis, restenosis after angioplasty and vein graft disease. The regulation of genes involved in VSM cell proliferation, particularly by naturally occurring inhibitors, is therefore of some importance. We have investigated the role of the c-myc proto-oncogene in growth arrest of exponentially proliferating rat VSM cells, following mitogen withdrawal, treatment with heparin (50 micrograms/ml), interferon-gamma (IFN-gamma) (100 i.u./ml), or the cyclic nucleotide analogues, 8-bromo-adenosine-3′5′-cyclic monophosphate (8-Br-cAMP; 0.1 mM) and 8-bromoguanosine-3′5′-cyclic monophosphate (8-Br-cGMP; 0.1 mM). Growth arrest was accompanied by down-regulation of c-Myc protein and mRNA following treatment with all inhibitors. Serum withdrawal or IFN-gamma treatment suppressed c-myc expression by more than 50% within 2 h, and this occurred throughout the cell cycle. Platelet-derived growth factor, epidermal ...
The concept of arterial SMC heterogeneity has gained wide acceptance in the last years.1 2 33 The distinct phenotypes of arterial SMCs have been mainly identified in vitro,4 5 6 7 8 9 10 11 12 13 14 15 16 suggesting that specific features of SMC populations arise and are maintained in the particular environment of cell culture. Hence, it was of interest to investigate whether in vitro SMC phenotypes are preserved when SMCs are placed back in an in vivo environment. For this purpose, we have implanted 2 SMC populations exhibiting distinct levels of differentiation in vitro into the rat carotid artery submitted to endothelial injury.24 25 The implanted SMCs were marked with PKH-26, a lipophilic cell membrane linker that is halved with each cell division but is not lost from the cell membrane.34 Our results show that the 2 implanted populations essentially retain for 20 days in vivo the phenotype that they specifically exhibited in vitro.. Spindle-shaped and epithelioid rat SMC populations have ...
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbeccos medium supplemented wi
Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
Jochen Meier Ivonne Strmel Radu Iosub Sonja Schmidt and Rosemarie Grantyn Developmental Physiology Johannes Mller Institute Humboldt University Medical School (Charit) Berlin Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance,Effectene,Transfection,Reagent,provides,efficient,gene,delivery,,,,,,,,,,,,to,primary,neuronal,cell,cultures,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Jacobs, B B., In vivo assay of function of mouse ovaries following culture in hormone enriched medium. (1963). Subject Strain Bibliography 1963. 546 ...
Full Text - Hydrogen peroxide (H2O2) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H2O2-treated human osteoblasts were tested. We show that H2O2 significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H2O2-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H2O2-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H2O2 induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124
Many studies have shown that TRAIL is a potent apoptosis inducer in malignant cells, whereas its role in normal cell physiology is much less well understood.1 We have demonstrated here that both aortic endothelial cells and HUVECs exhibit a similar pattern of surface TRAIL-R expression. Moreover, in vascular endothelial cells, TRAIL stimulates the phosphorylation of the serine/threonine kinase Akt in a manner dependent on PI3K activation.14 The ability of TRAIL to activate the antiapoptotic PI3K/Akt pathway in endothelial cells is a completely new and unexpected finding, also in consideration of the large number of studies underlining the proapoptotic activity of TRAIL, at least in malignant cells.1,4,5 It should be underlined that the PI3K/Akt pathway is of central importance in endothelial cell biology, conferring survival to endothelial cells in response to angiogenic cytokine stimulation, fluid shear stress, and matrix attachment signals. This pathway is essential also for endothelial cell ...
TY - JOUR. T1 - Activation of caspase-8 and Erk-1/2 in domes regulates cell death induced by confluence in MDCK cells. AU - Chang, Yung Heng. AU - Lin, Hsi Hui. AU - Wang, Yang Kao. AU - Chiu, Wen Tai. AU - Su, Hsiao Wen. AU - Tang, Ming Jer. PY - 2007/4/1. Y1 - 2007/4/1. N2 - Under normal culture conditions, cells adhere to culture dish, spread out, proliferate, and finally cover all areas and reach confluence. During the confluent stage, cell proliferation ceases and differentiation is enhanced. Meanwhile, cell death also appears as the monolayer confluence proceeds. To delineate the mechanism of cell death induced by the confluent process, we employed Madin-Darby canine kidney (MDCK) cells. When approaching confluence, MDCK cells exhibited increase the levels of caspase-2 and enhanced the activity of caspase-8. Using various caspase inhibitors to block apoptosis, we found that only z-VAD-fmk and z-IETD-fmk can inhibit confluent cell death, indicating that confluent cell death is mediated by ...
In the present article, we compared the antiangiogenic activity of trans- and cis-resveratrol on endothelial cells of different origin activated by endogenous or exogenous stimuli. The results indicate that trans-resveratrol affects endothelial cell proliferation and various cell proliferation-independent aspects of the angiogenesis process, including endothelial cell sprout formation, collagen gel invasion, and morphogenesis. Thus, trans-resveratrol acts as an angiostatic molecule on multiple targets of the angiogenic process. In all the assays, the cis stereoisomer exerted only a limited effect, if any. In keeping with the in vitro observations, trans-resveratrol was more effective than the cis isomer in inhibiting angiogenesis and tumor growth in vivo.. The molecular bases of the antiangiogenic activity of resveratrol are poorly understood (16). trans-Resveratrol is endowed with a significant antioxidant and anti-inflammatory activity (8, 9). However, no direct relationship exists between the ...
TY - JOUR. T1 - Recombinant murine and human IL 1α bind to human endothelial cells with an equal affinity, but have an unequal ability to induce endothelial cell adherence of lymphocytes. AU - Thieme, T. R.. AU - Hefeneider, S. H.. AU - Wagner, C. R.. AU - Burger, D. R.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - Consistent with the reports of others, we have demonstrated that human peripheral blood lymphocytes adhere to cultured human umbilical vein-derived endothelial cells (EC) in vitro. In our studies adherence was increased twofold to threefold by a 6-hr preincubation of the EC with IL 1. Recombinant human IL 1α induced a maximal adherence response at less than 1 U per 2 x 104 EC. In contrast, recombinant murine IL 1α was found to be 250- to 1250-fold less active in the adherence assay, based on units of IL 1 activity defined by the murine thymocyte proliferation assay. Moreover, when EC were preincubated with excess murine IL 1, no inhibition of the adherence-inducing effect of human IL 1 was ...
The effects of interferons (IFNs) on lymphocyte adhesion to cultured human vascular endothelial cells (EC) were investigated using an in vitro assay. Endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (CM) from 12-O-tetra decanoylphorbol 13-acetate-concanavalin A (TPA-Con A) stimulated human peripheral blood lymphocytes (PBL), or with recombinant (r) gamma interferon (IFN-gamma) or r alpha interferon (IFN-alpha), and then were incubated with freshly isolated PBL. Natural IFN-gamma in the TPA-Con A CM and rIFN-gamma (12.5-500 U/ml) induced major histocompatibility complex-class II antigens (HLA-DR, HLA-DP, and HLA-DQ) and significant lymphocyte adhesion to the EC, whereas rIFN-alpha did not. The lymphocyte adhesion to the EC and the expression of DR antigens on the EC were well correlated in terms of both kinetics and the dose-response pattern of rIFN-gamma. When EC expressing I region associated (Ia) antigen were preincubated with ...
The fully sequenced pCERI is transformed into E.coli BL21 and E.coli Nissle 1917 that are used for the characterization of our regulatory system. The expression efficiency of the genes of interest is characterized by a fluorescence-based assay (Results) Overnight cultures of both recombinant strains are inoculated with single colonies. The overnight cultures are diluted with LB-Amp to an OD of 0.02. 200 μ of the dilutions are pipetted in microtiter plates and incubated at 37 °C in the plate reader. Every 15 minutes mRFP fluorescence (Ex. 584 nm, Em. 607 nm), CFP fluorescence (Ex. 439 nm, Em. 476) and OD600 is measured over a period of 13 h. The measured RFU (relative fluorescence unit) is either blotted against time or against OD600 to evaluate the expression behavior of pCERI. Additionally several samples are either induced with 0.1 -50 nM of 3OC6-HSL and 0.1-100 nM of C8-HSL to explore putative advantages of higher HSL-synthesis, that might be possible by positioning each HSL-synthase, ...
This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for ...
Choice of AMP 100 versus AMP 50 - posted in Molecular Cloning: Ok, i know the main difference is the amp concentration. But i wonder if it matters that i use amp 50 for overnight culture versus amp 100 for overnight culture.
I am studying the differentiation of leukemic cells along the monocytic lineage using calcitriol as differentiative agent and others vitamins as possible stimulating compounds on differentiation. In my work I have used HL-60 cells as in vitro culture cells but now I have some problems because these cells have changed their response and develop expontaneous differentiation in normal culture conditions. Now, I am thinking on NB4 cells as a better cell line to study differentiation. I have ask some information to cell banks as ATCC and ECA but they dont have this cell line. Could anybody tell me if anyone can send me this cell line?, I have very gratefull if anyone could send me HL-60 cells too. Waiting positive responses and acknowledging in advance. Guillermo Lopez-Lluch Departamento de Biología Celular Facultad de Ciencias Universidad de Córdoba Avda. S. Alberto Magno S/N E-14007 Córdoba, Spain. TFNO: Int + 34-57-218634 FAX: Int + 34-57-218634 e-mail: bc2biced at ...
TY - JOUR. T1 - Cell interactions in nerve and muscle cell cultures.. AU - Christian, C. N.. AU - Bergey, G. K.. AU - Daniels, M. P.. AU - Nelson, P. G.. PY - 1980/12/1. Y1 - 1980/12/1. N2 - The neurotransmitter synthesized by a given class of neurones is subject to modification and, indeed, a qualitative switchover in transmitter biochemistry recently has been demonstrated (Furshpan, POtter & Landis, 1980; Walicke, Campenot & Patterson, 1977). In conjunction with the specification of transmitter biosynthesis that becomes established in a given neurone, a complementary specification of appropriate receptor production is required in any cell functionally post-synaptic to that neurone. An additional requirement of peculiar force in the nervous system has to do with the spatial organization of the receptors in the surface membrane of the post-synaptic cell once the receptors are synthesized. Inappropriately distributed receptors are useless receptors. The perfect registration of a variety of types ...
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TY - JOUR. T1 - Studies on the mechanism of hrmonal stimulation of zinc uptake in human cell cultures. T2 - hormone-cell interactions and characteristics of zinc accumulation.. AU - Cox, R. P.. AU - Ruckenstein, A.. PY - 1971/2. Y1 - 1971/2. UR - UR - M3 - Article. C2 - 4322943. AN - SCOPUS:0015012917. VL - 77. SP - 71. EP - 81. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 1. ER - ...
One group of projects in the lab concerns co-receptors, cell-surface molecules that regulate the interaction between soluble growth factors and their cell-surface receptors. We have identified a novel co-receptor for BMPs (bone morphogenetic proteins) and are studying the roles of Heparan Sulfate Proteoglycans (HSPGs) as co-receptors for a variety of growth factors, including those that drive tumor development and pattern the developing nervous system. We have used knockout technology to generate mice that are mutant for particular HSPGs, and are also working with mice that are mutant for some of the biosynthetic enzymes required to make HSPGs. We are also using modeling approaches to understand how HSPGs influences the kinetic parameters of growth factor-receptor interaction ...
Were stained with rat endothelial cell antigen. + pixels were counted in glomeruli and tubular fields working with ImageJ Application . To be able to evaluate
How would the cells respond to Aβ? Mancuso injected 5 μL of 10 μM synthetic oligomers into the mouse brain ventricles eight to 10 weeks after transplanting the human microglia. In reaction to this insult, endogenous mouse microglia shifted their transcriptomes sequentially from homeostatic to cytokine responsive, to activated, with the latter partially overlapping with disease-associated microglial (DAM) signatures described previously (Jun 2017 news). Human microglia in the mice underwent a transcriptional transformation as well, but analysis of more than 10,000 orthologous genes indicated poor correlation between human and mouse microglial responses. Of 207 differentially activated genes, 112 were up in human but not mouse microglia; they included GWAS hits BIN1 and PICALM. We saw that human microglia responded very differently to Aβ oligomers. This emphasizes the need to look specifically at human cells in the context of AD, said De Strooper.. Blurton-Jones and colleagues took a ... Amruta Phatak, Mohammad Athar, James A. Crowell, David Leffel, Brittney-Shea Herbert, Allen E. Bale, Levy Kopelovich
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Recent evidence supports a strong role for immune suppression in facilitating cancer progression. Transforming growth factor beta (TGFß) regulates a diverse array of biological events, including neoangiogenesis, stromal formation, and immune responses. Both TGFß and a small molecule inhibitor of TGFßR-1, LY364947, were effective regulators of these processes in vitro. Using quantitative RT-PCR and fluorescence imaging, TGFß increased collagen production by pancreatic stellate cells while addition of LY364947 suppressed production below basal levels. Endothelial cell proliferation, measured as a function of endothelial tube formation in Geltrex® matrix, was similarly increased by TGFß and reduced to basal levels by LY364947. To explore the in vivo effects of these molecules, LY364947 was encapsulated in liposomes to encourage tumor accumulation and to avoid administration of DMSO vehicle. Liposomes were prepared with a molar ratio of 6:3:1 1,2-dipalmitoyl-sn-glycero-3-phosphocholine ...
Simple muscle cell cultures are utilized frequently to investigate the mobile mechanisms of contraction. in Personal computer and RC vs .. Me personally and FC (all < 0.05). Appearance of Gi3, serine/threonine proteins phosphatase-1 -catalytic subunit, and Rho kinase 1 improved in Personal computer and RC vs .. Me personally and FC (all < 0.05). Cell tradition and resuspension downregulated appearance of -actin and calponin, but not really myosin weighty string. The online impact of these molecular adjustments was reductions of cell reactivity to ACh in RC vs .. FC. Overexpression of CPI-17 in Personal computer partly reversed the reductions of contractility in resuspended cells. Methylation-specific PCR demonstrated improved methylation of the gene marketer in Computer vs .. Me personally (< 0.05). We agreed that even muscles cells preserve their contractile phenotype in lifestyle. Nevertheless, reactivity to ACh diminishes because of changed reflection of particular cell-signaling protein ...
Conventional biology expects the process of mammalian cell division, mitosis, to occur by the equal partition of a mother cell into two daughter cells. Bioengineers at UCLA Engineering have developed a platform that mechanically ...
A growing body of evidence suggests that transiently activated NF-κB in noninflammatory states and persistently activated NF-κB during inflammation play different pathophysiological roles in vivo. During the initial phase of inflammation, proinflammatory cytokines and mediators induce prolonged NF-κB activation in various inflammatory cells and endothelium. The activated NF-κB further upregulates the expression of several proinflammatory molecules, such as endothelial cell adhesion molecules and macrophage inflammatory protein-2, which triggers neutrophilic infiltration and tissue injury (1, 27, 34). Thus persistent NF-κB activation during the early phase of inflammation amplifies inflammatory response in vivo.. In contrast, transient activation of NF-κB before inflammatory stimulation results in the anti-inflammatory response. For example, several investigators (41, 54, 56) have found that transient activation of NF-κB is required for the heart to tolerate ischemia-reperfusion-induced ...
Time-dependent induction of connected cells by overexpression of cdc5ΔN. (A) Strain KLY1083 expressing three copies of GAL1-EGFP-cdc5ΔN homogeneously induced
floating THP-1 cells - medium change advice - posted in Tissue and Cell Culture: Hello, I was adamantly asked to culture THP-1 cells and did so, by thawing a cryovial and seeding the cells in a 25 cm flask. 3 days later 99% of cells are floating, which I reckon is OK. Isnt it? So, do I need to change the medium before they reach ~8x 10^5 cells/ml and are ready to be passed? Since these are floating cells, how do I do that? Also, would a 1/3 split ratio be OK for these cells? Th...
1) The main issue with this paper is this: these cell lines, although originally human, are all immortalized cancer cell lines characterised by markedly different biological properties when compared to normal human cells of the same tissue/cell type. They cant be readily used a surrogates for normal human tissue/cell types. None were primary cells nor were any even from recently acquired tissue samples from biopsies etc. People have infected primary human airway epithelial cultures with hCoV-EMC - so this can be done successfully - , although it would be more difficult for other tissue types as these cultures havent been developed. Some of these cell lines used may by chance lack key viral repressors of infection present in normal primary cells, which could skew results from cell culture infection experiments. Plus, a human tissue is not just a single cell type - they are composed of diverse kinds of cells that could together behave much, much differently than cell lines in culture ...
Accepted name: raucaffricine β-glucosidase. Reaction: raucaffricine + H2O = D-glucose + vomilenine. For diagram click here.. Other name(s): raucaffricine β-D-glucosidase; raucaffricine glucosidase. Systematic name: raucaffricine β-D-glucohydrolase. Comments: Highly specific; some other ajmalan glucoside alkaloids are hydrolysed, but more slowly.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 102925-37-1. References:. 1. Schübel, H., Stöckigt, J., Feicht, R. and Simon, H. Partial-purification and characterization of raucaffricine β-D-glucosidase from plant cell-suspension cultures of Rauwolfia serpentina benth. Helv. Chim. Acta 69 (1986) 538-547.. ...
Increasing numbers of studies have demonstrated that PGE1 plays an important role in determining the survival of cells exposed to H/R conditions. PGE1 treatment of liver sinusoidal endothelial cells increases their survival and resistance to apoptosis during H/R injury by suppressing NO production and MMP release [31]. Interestingly, a previous study by Ma et al. [32] reported that the total number of cardiomyocytes was decreased in an H/R group when compared with a group of cardiomyocytes pretreated with PGE1, and the mechanism for that effect probably involved a decrease in Bax expression and an increase in Bcl-2 expression. Caspase 3, which is downstream of Bcl-2 and Bax, is considered to be a critical protease required for apoptosis [39]. In the present study, we observed that inhibition of apoptosis by PGE1 in H/R injured cells involved the inactivation of cleaved-caspase-3. Our results support a model in which PGE1 inhibits apoptosis in cardiomyocytes, at least in part, via a ...
Finally, our article on human and mouse neurons has been published! The picture above shows the neurons of a human and a mouse. Human and mouse neurons are similar overall, but they also have…
CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics ...
483279889 - EP 3108256 A4 2017-10-04 - METHOD OF DETECTING CANCER - [origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.[origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.
Results ICAM-1 expression was significantly up-regulated upon the introduction of TNF-α under all conditions in HUVEC (figure 1). However, baseline expression was increased when co-cultured with both NHDF (2.0 vs 1.3, p,0.001) and HUASMC (6.5 vs 1.3, p,0.001). This meant that ICAM-1 expression at 12 hours was also significantly higher in co-culture with NHDF (8.3 vs 5.2, p,0.001) and HUASMC (11.0 vs 5.2, p,0.001). Moreover, there was a moderate relationship between HUVEC ICAM-1 expression and the cell ratio when in co-culture with NHDF, where decreasing NHDF resulted in decreased ICAM-1 in HUVEC (R2=0.45).. TNF-α caused an increase in ICAM-1 expression in NHDF under monoculture conditions (Fig. 2); this up-regulation was significantly reduced in co-culture conditions with HUVEC (1.7 vs 5.1, p,0.001). A similar trend was observed when in co-culture with HUASMC (2.7 vs 5.1, p,0.001), except the baseline expression of ICAM-1 was also increased (2.8 vs 1.1, p,0.001).. Constitutive production of ...
Mouse IL-10 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
Mouse IL-4 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
2010) (Fig. 1A). Fibroblasts were seeded at 1.5 × 105 cells/filter and HUVEC were seeded at 1.0 × 105 cells/filter to yield confluent monolayers within 24 h. After 24 h, culture media were removed and the 24-well inserts were fitted into the 12-well inserts, with 200 μl fibroblast medium added to the surface of each filter and 1.5 ml to the lower chamber. Cells were co-cultured together for 48 h, with 100 U/ml TNF alpha (R&D Systems, Abingdon, UK) in combination with 10 ng/ml IFN gamma (Peprotech Inc., London, UK) added for the second 24 h when desired. For. comparison, parallel cultures of HUVEC or fibroblasts were maintained alone on their Selleckchem AC220 original filters. To form collagen gels, ice-cold rat-tail type 1 collagen PD-166866 cell line dissolved in acetic acid (2.15 mg/ml; First Link Ltd, West Midlands, UK) was mixed with ice cold 10 × concentrated M199 in the ratio 830:170 and the pH was neutralised by addition of ice cold 1 N NaOH. For each 1 ml of gel, 160 μl FCS was ...
You know its funny that you mention tube like structures...... do they look fairly long and rectangular? I have something growing in tissue culture that looks like that. These cells started off as hemangiomas. We used collangenase to break it up, and are growing these cells in DMEM with added penicillin/streptomyacin and fetal bovine serum. Ive seen quite a few of these, one person thinks they look like myocytes....heart muscle cells in particular, they arent beating or anythying though. And if that is what they are, id be really really surprised. Most of the cells look like fibroblasts (Im thinking that what look like fibroblasts might be pericytes) with some scattered possible endothelial cells. We transfected a few dishes with a vector to induce division, but we dont see these cells in any of the tissue culture dishes containing the transformed cells. I wonder if we are looking at the same thing. Ive ordered a camera for our inverted scope, ill have to take a picture when it comes in. ...
The characterization was performed using overnight cultures of the corresponding part. The optical density of the cell cultures was adjusted to 0.4 and 50 µl of the culture sample was pipette onto a microscope slide and a coverslip was placed over it. A 100X/1.3 Oil immersion objective was used for the light microscopy imaging and filters necessary for CFP fluorescence detection was set. An initial live imaging was done in transmitted light mode to find a field of view with maximum number of cells. The same was then used for capturing the fluorescence signal of the CFP. The same procedure was repeated several times in order to quantify the data. If we need to observe more number of cells under a given field of view, it is recommended to spin down the cell culture and use the resuspended pellet for increased cell density.. ...
The characterization was performed using overnight cultures of the corresponding part. The optical density of the cell cultures was adjusted to 0.4 and 50 µl of the culture sample was pipetted onto a microscope slide and a coverslip was placed over it. A 100X/1.3 Oil immersion objective was used for the light microscopy imaging and filters necessary for CFP fluorescence detection was set. An initial live imaging was done in transmitted light mode to find a field of view with maximum number of cells. The same was then used for capturing the fluorescence signal of the CFP. The same procedure was repeated several times in order to quantify the data. If we need to observe more number of cells under a given field of view, it is recommended to spin down the cell culture and use the resuspended pellet for increased cell density.. ...
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Cell culture[edit]. Puromycin is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic ... Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures ... "Mol Biol Cell. 8 (8): 1559-1573. doi:10.1091/mbc.8.8.1559. PMC 276176. PMID 9285825.. ... "In Hopsu-Havu, Väinö K.; Järvinen, Mikko; Kirschke, Heidrun (eds.). Proteolysis in Cell Functions. IOS Press. pp. 88-95. ISBN ...
... sometimes before other cultures (e.g. blood cultures and sputum cultures) become positive. A Gram stain may give a rough ... Cell count and differential[edit]. The number of white blood cells can give an indication of infection. The specific subtypes ... Cultures and stains[edit]. If the effusion is caused by infection, microbiological culture may yield the infectious organism ... In the case of blood: it will contain some or all plasma proteins, white blood cells, platelets and (in the case of local ...
Cell culture[edit]. Mineral oil of special purity is often used as an overlay covering microdrops of culture medium in petri ... The use of oil presents several advantages over the open culture system: it allows for several oocytes and embryos to be ... as an adjuvant to stimulate a cell-mediated immune response to the vaccinating agent.[citation needed] In the poultry industry ... as few as 20 microlitres per oocyte instead of several millilitres for the batch culture); and it serves as a temperature ...
Cell culture[edit]. Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering ... Cell culture (cell-based) manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines ... In contrast, cell culture manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines (e ... AVI Bio Pharma Inc. has evidence of inhibition of multiple subtypes of influenza A virus in cell culture with Morpholino ...
10.1 Cell-of-origin. *11 Society and culture. *12 Research *12.1 CRPC ... lo Prostate Cancer Cell Population Harbors Self-Renewing Long-Term Tumor-Propagating Cells that Resist Castration". Cell Stem ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
Cell culture. *Model organisms (such as C57BL/6 mice). *Methods *Nucleic acid ... Up-regulation is a process that occurs within a cell triggered by a signal (originating internal or external to the cell), ... Cell. 62 (5): 712-27. doi:10.1016/j.molcel.2016.04.006. PMC 5476208 . PMID 27259203.. ... Cells do this by modulating the capping, splicing, addition of a Poly(A) Tail, the sequence-specific nuclear export rates, and ...
Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... culture worldwide via inhalation or skin lesion Babesiosis Babesia B. divergens, B. bigemina, B. equi, B. microfti, B. duncani ... culture worldwide contact lenses cleaned with contaminated tap water Granulomatous amoebic encephalitis Balamuthia mandrillaris ... red blood cells Giemsa-stained thin blood smear New England (different species have worldwide distribution) tick bites, e.g. ...
"Labeling Cultured Cells with32Piand Preparing Cell Lysates for Immunoprecipitation". Labeling Cultured Cells with 32Pi and ... ACK is used for lysis of red blood cells in biological samples where other cells such as white blood cells are of greater ... "Lysis of Cultured Cells for Immunoprecipitation". Cold Spring Harbor Protocols. 2010 (8): pdb.prot5466. doi:10.1101/pdb. ... "Detergents for Cell Lysis and Protein Extraction". Retrieved 2016-03-16.. ...
... mammalian cell cultures; vegetables; human nostrils and throats; and human and animal brain, skin, and lung tissues. B. ...
Cell behavior[edit]. Main article: Microfluidic cell culture. The ability to create precise and carefully controlled ... cell separation, in particular, blood cell separation, protein analysis, cell manipulation and analysis including cell ... Microfluidic cell culture. References[edit]. *^ S.C.Terry, J.H.Jerman and J.B.Angell:A Gas Chromatographic Air Analyzer ... Fuel cells[edit]. Further information: Electroosmotic pump. Microfluidic fuel cells can use laminar flow to separate the fuel ...
Cell cultureEdit. Petri dishes are also used for cell cultivation of isolated cells from eukaryotic organisms, such as in ... alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to culture ... Axenic Cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish ... In the latter, the cells often grow as a layer attached to the bottom surface of the dish, below the culture medium.[25] ...
"Cell Stem Cell. 10 (5): 556-69. doi:10.1016/j.stem.2012.03.009. PMC 3348510 . PMID 22560078.. ... Society and cultureEdit. People with prostate cancer generally encounter significant disparities in awareness, funding, media ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
Cells are oval-shaped, non-motile and stain Gram-negative. Strictly anaerobic. Chemo-organotrophic. Mucolytic in pure culture.[ ... "Cell Metabolism. 22 (4): 658-68. doi:10.1016/j.cmet.2015.07.026. PMC 4598654. PMID 26321659. Mice that received microbiota from ...
Kampf C; Roomans GM (May 2001). "Effects of hypochlorite on cultured respiratory epithelial cells". Free Radic. Res. 34 (5): ... Nitric oxide can diffuse through the plasma membrane into neighbouring cells, allowing cell signalling, so nitroindazole ...
While the long-term potentiation of synapses in cell culture seems to provide an elegant substrate for learning and memory, the ... The synaptic tagging hypothesis attempts to solve the cell's difficult problem of synthesizing proteins in the cell body but ... When an axon of cell A is near enough to excite a cell B and repeatedly or persistently takes part in firing it, some growth ... in cells of the dentate gyrus. What Lømo unexpectedly observed was that the postsynaptic cells' response to these single-pulse ...
"BRCA1 inhibition of telomerase activity in cultured cells". Molecular and Cellular Biology. 23 (23): 8668-90. PMC 262673 . PMID ... cell cycle arrest. • positive regulation of response to DNA damage stimulus. • positive regulation of cell proliferation. • ... "Cell. 142 (3): 480-93. PMC 2923036 . PMID 20691906. doi:10.1016/j.cell.2010.06.037.. ... "Cell. 151 (1): 68-79. PMC 3471363 . PMID 23021216. doi:10.1016/j.cell.2012.08.033.. ...
Phosphate buffered saline (cell culture). *Tyrode's solution. References[edit]. *^ a b c British national formulary: BNF 69 (69 ...
Narayanaswamy, S. (1994). Plant Cell and Tissue Culture. Tata McGraw-Hill Education. pp. vi. ISBN 978-0-07-460277-5. .. ... Embryonic stem cells incorporate the altered gene, which replaces the already present functional copy. These stem cells are ... As only a single cell is transformed with genetic material, the organism must be regenerated from that single cell. In plants ... DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope ...
For euglenids to reproduce, asexual reproduction takes place in the form of binary fission, and the cells replicate and divide ... This varies from rigid to flexible, and gives the cell its shape, often giving it distinctive striations. In many euglenids the ... which are excavate eukaryotes of the phylum Euglenophyta and their cell structure is typical of that group. They are commonly ... The pellicle is composed of proteinaceous strips underneath the cell membrane, supported by dorsal and ventral microtubules. ...
... are commonly used in cell culture systems to maintain stem and precursor cells in an undifferentiated state during cell culture ... Cell adhesion[edit]. Many cells bind to components of the extracellular matrix. Cell adhesion can occur in two ways; by focal ... Plant cells are tessellated to form tissues. The cell wall is the relatively rigid structure surrounding the plant cell. The ... Fibronectins bind collagen and cell-surface integrins, causing a reorganization of the cell's cytoskeleton to facilitate cell ...
"Acoustic microscopy of cultured cells. Distribution of forces and cytoskeletal elements". Cell Biophys. 18 (3): 279-93. doi: ... "Mechanical basis of cell shape: investigations with the scanning acoustic microscope". Biochem. Cell Biol. 73 (7-8): 337-48. ... SAM can provide data on the elasticity of cells and tissues, which can give useful information on the physical forces holding ... Hildebrand JA; Rugar D; Johnston RN; Quate CF (1981). "Acoustic microscopy of living cells". Proc. Natl. Acad. Sci. U.S.A. 78 ( ...
Cell and tissue physiology • Fundamental health economics • Health policy and public welfare • Academic reading and writing for ... health sciences • Selective study in language subject • World culture • Medical ethics and professional law • Free elective ...
... researchers insert a tiny electrode into the brain to monitor the activity of a single cell. If 90 percent of cells were unused ... In popular culture[edit]. Several books, films, and short stories have been written closely related to this myth. They include ... Synaptic pruning: Brain cells that are not used have a tendency to degenerate. Hence if 90 percent of the brain were inactive, ... Although parts of the brain have broadly understood functions, many mysteries remain about how brain cells (i.e., neurons and ...
Stem cell genomics: Helps in stem cell biology. Aim is to establish stem cells as a leading model system for understanding ... Culture[edit]. Inspired by foundational questions in evolutionary biology, a Harvard team around Jean-Baptiste Michel and Erez ... "Omicum": Building of the Estonian Biocentre which houses the Estonian Genome Centre and Institute of Molecular and Cell Biology ... Nucleomics: Study of the complete set of genomic components which form "the cell nucleus as a complex, dynamic biological ...
Cell culture: Human or animal cell cultures are infected with the microbe of interest. These cultures are then observed to ... These are primarily used to culture bacteria and fungi.. *Liquid culture: Cells are grown inside a liquid media. Microbial ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ... Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ...
... which has been found in one study to be mutagenic and genotoxic in cell cultures which do not respond to β-carotene itself.[30] ... "Toxicity of oxidized β-carotene to cultured human cells". Experimental Eye Research. 81 (2): 239-243. doi:10.1016/j.exer. ...
G. Diets, Culture Media, Food Supplements. Vol. 4. Culture Media for Cells, Organs and Embryos. Cleveland, OH: CRC Press, 1977 ... G. Diets, Culture Media, Food Supplements. Vol. 3. Culture Media for Microorganisms and Plants. Cleveland, OH: CRC Press, 1978 ... G. Diets, Culture Media, Food Supplements. Vol. 1. Dits for Mammals. Cleveland, OH: CRC Press, 1977. 645 pp. CRC Handbook ... G. Diets, Culture Media, Food Supplements. Vol. 2. Food Habits of and Diets for Invertebrates and Vertebrates. Zoo diets. ...
Inhibitory effect on cultures tumor cell 5 Erythropoietin Kidney Stimulate development of erythropoietic cells ... Stimulate growth of mesenchymal cells, promotes wound healing 3 Transforming growth factor (Alpha) Epithelial cell Similar to ... cell membrane. Biological process. • negative regulation of epidermal growth factor receptor signaling pathway. • positive ... regulation of cell motility. • activation of MAPKK activity. • positive regulation of receptor internalization. • positive ...
Predieri, S. (2001). "Mutation induction and tissue culture in improving fruits". Plant Cell, Tissue and Organ Culture. 64 (2/3 ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... Koornneef, M.; Meinke, D. (2010). "The development of Arabidopsis as a model plant". The Plant journal : for cell and molecular ... DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under ...
Ader M., Tanaka E. M. (2014). "Modeling human development in 3D culture". Current Opinion in Cell Biology. 31: 23-28. doi: ... This occurs when individual cells or groups of cells grow longer. Not all plant cells will grow to the same length. When cells ... These cell divisions are usually rapid with no growth so the daughter cells are half the size of the mother cell and the whole ... A single tissue, formed from a single type of progenitor cell or stem cell, often consists of several differentiated cell types ...
If the stem cells and sebaceous gland are destroyed, there is then no possibility for regeneration of the hair follicle, and ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ... The goal of treatment is to decrease or eliminate the lymphocytic inflammatory cells that are attacking and destroying the hair ... However, all cicatricial alopecias involve inflammation directed at the upper part of the hair follicle where the stem cells ...
The brain detects insulin in the blood, which indicates that nutrients are being absorbed by cells and a person is getting full ... The issue of healthy eating has long been an important concern to individuals and cultures. Among other practices, fasting, ... Eating positions vary according to the different regions of the world, as culture influences the way people eat their meals. ... When the glucose levels of cells drop (glucoprivation), the body starts to produce the feeling of hunger. The body also ...
Nebert, D.W. and Gelboin, H.V. (1968). "Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and ... Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins ( ...
Micropropagation of Psiadia arguta through cotyledonary axillary bud culture». Plant Growth Regulation. doi:10.1023/A: ... In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected ...
The cells met to read Marxist texts and hold self-criticism sessions.[51] Sâr joined a cell that met on the rue Lacepède; his ... Sâr made little or no attempt to assimilate into French culture[46] and was never completely at ease in the French language.[41 ... They established party cells, emphasising the recruitment of small numbers of dedicated members, and organized political ... which in Cambodian culture was traditionally associated with monkhood.[256] ...
Invasins, such as pneumolysin, an antiphagocytic capsule, various adhesins, and immunogenic cell wall components are all major ... Diagnosis is generally made based on clinical suspicion along with a positive culture from a sample from virtually any place in ... and white blood cells to fill the alveoli. This condition is called pneumonia.[20] It is susceptible to clindamycin.[21] ... Optochin sensitivity in a culture of Streptococcus pneumoniae ...
They are a class of cell cycle-specific cytotoxic drugs that work by inhibiting the ability of cancer cells to divide: Acting ... Production of Vindoline and Catharanthine in Multiple Shoot Cultures". In Bajaj, Y. P. S. Biotechnology in Agriculture and ... The newer semi-synthetic chemotherapeutic agent vinorelbine is used in the treatment of non-small-cell lung cancer[7][9] and is ... "Safety and efficacy of vinorelbine in the treatment of non-small cell lung cancer". Clinical Medicine Insights: Oncology. 5 ...
B. C. Giovanella u. a.: Development of invasive tumors in the nude mouse after injection of cultured human melanoma cells. In: ... D. Kong u. a.: Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude ... L. Miers u. a.: Implantation of different malignant human cell lines in an athymic mouse does not alter success and growth ... T. Devos u. a.: Occurrence of autoimmunity after xenothymus transplantation in T-cell-deficient mice depends on the thymus ...
Researchers looking at slides of cultures of cells that make monoclonal antibodies. These are grown in a lab and the ... dendritic cells and other cells including liver cells, fibroblasts, and adrenal gland cells.[93] Viral replication triggers ... doi:10.1016/j.cell.2014.10.006. PMC 4243531. PMID 25417101.. *^ a b c d e f g h Kühl A, Pöhlmann S (September 2012). "How Ebola ... liver cells, and several types of immune cells such as macrophages, monocytes, and dendritic cells are the main targets of ...
Cornelius AS, Yerram NR, Kratz DA, Spector AA: Cytotoxic effect of cis-parinaric acid in cultured malignant cells. Cancer Res. ...
Low operating voltages compatible with batteries of only a few cells.. *Circuits with greater energy efficiency are usually ... "groundbreaking invention that transformed life and culture around the world".[62] Its importance in today's society rests on ... Solaristor (from solar cell transistor), a two-terminal gate-less self-powered phototransistor. ...
... and Th1 cells.[45] IL-1α stimulates increased skin cell activity and reproduction, which, in turn, fuels comedo development.[45 ... Society and culture[edit]. The costs and social impact of acne are substantial. In the United States, acne vulgaris is ... and accumulation of skin cells in the hair follicle.[1] In healthy skin, the skin cells that have died come up to the surface ... the increased production of oily sebum causes the dead skin cells to stick together.[10] The accumulation of dead skin cell ...
微生物培養鑑定(英語:Microbiological culture). *血清學 ... 細胞損傷(英語:Cell damage). *傷口
Porter K.R; Claude A. & Fullam E.F. (1945). "A study of tissue culture cells by electron microscopy". J Exp Med. 81 (3): 233- ... The endoplasmic reticulum is in cells that have a nucleus: in eukaryote cells but not in prokaryote cells. It takes these forms ... Similar to the ER is the sarcoplasmic reticulum (SR) found only in muscle cells. The SR stores and pumps calcium ions. The SR ... contains large stores of calcium, which it releases when the muscle cell is stimulated.[1] Another type of cytoplasmic network ...
Polymorphonuclear cells also infiltrate the epithelium, and chronic inflammatory cells infiltrate the lamina propria. Atrophic ... Oral swabs are taken if culture is required. Some recommend that swabs be taken from 3 different oral sites. Oral rinse ... Apart from true hyphae, Candida can also form pseudohyphae - elongated filamentous cells, lined end to end. As a general rule, ... Smears and biopsies are usually stained with periodic acid-Schiff, which stains carbohydrates in fungal cell walls in magenta. ...
... has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ... a novel potent inhibitor of signal transduction and growth in vitro and in vivo in small cell lung cancer cells". Cancer ...
... during which neither showed traces of HIV in their blood plasma and purified CD4 T cells using a sensitive culture method (less ... who have lost their stem cells after birth. Other conditions[13] treated with stem cell transplants include sickle-cell disease ... Peripheral blood stem cells[26] are now the most common source of stem cells for HSCT. They are collected from the blood ... Sources and storage of cells[edit]. To limit the risks of transplanted stem cell rejection or of severe graft-versus-host ...
Technoscience and Environmental Justice: Expert Cultures in a Grassroots Movement. MIT Press. pp. 1-18. ISBN 978-0262015790.. ... In medicine, this era brought innovations such as open-heart surgery and later stem cell therapy along with new medications and ... Hughes, Thomas P. (2004). Human-Built World: How to Think About Technology and Culture. Chicago: University of Chicago Press. ... Technoscience and Environmental Justice: Expert Cultures in a Grassroots Movement. MIT Press. pp. 229-48. ISBN 978-0262015790. ...
In a Dalmau plate culture containing cornmeal agar and Polysorbate 80, K. marxianus forms a rudimentary to branched ... The formation of the ascospores occurs through the conjugation of the haploid cells preceding the formation of the ascus.[8] ... Mukherjee, A; Pramanik, S; Das, D; Roy, R; Therese, KL (2014). "Polymicrobial chronic endophthalmitis diagnosed by culture and ... the yeast cells appear globose, ellipsoidal or cylindrical, 2-6 x 3-11 μm in size.[6] In a glucose-yeast extract broth, K. ...
The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ... Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... Other colour-changing cells are reflective iridophores and white leucophores.[93] This colour-changing ability is also used to ... The lens is suspended behind the pupil and photoreceptive retinal cells cover the back of the eye. The pupil can be adjusted in ...
In cultured mammalian cells, such as the Chinese hamster ovary cell line, a number of genetic loci are present in a functional ... "Evidence obtained by segregation analysis for functional hemizygosity at the Emtr locus in CHO cells". Cell. 14: 1007-1013. doi ... A cell is said to be homozygous for a particular gene when identical alleles of the gene are present on both homologous ... A chromosome in a diploid organism is hemizygous when only one copy is present.[2] The cell or organism is called a hemizygote ...
Society and culture[edit]. Economics[edit]. As of 2011, levothyroxine was the second most commonly prescribed medication in the ... T4 and T3 bind to thyroid receptor proteins in the cell nucleus and cause metabolic effects through the control of DNA ...
... induces a calcium-dependent current in cultured dorsal root ganglion cells". J. Neurophysiol. 85 (3): 1340-5. doi:10.1152/jn. ... This seems to occur via immune cells interacting with the peripheral nervous system and releasing pain-producing chemicals ( ... "Regulation of proinflammatory cytokines gene expression by nociceptin/orphanin FQ in the spinal cord and the cultured ...
The isolated swarmer cells can then be grown as a synchronized cell culture. Detailed study of the molecular development of ... Role of the swarmer cell stageEdit. The Caulobacter stalked cell stage provides a fitness advantage by anchoring the cell to ... Swarmer cells differentiate into stalked cells after a short period of motility. Chromosome replication and cell division only ... What is the offsetting fitness advantage of this motile cell stage? The swarmer cell is thought to provide cell dispersal, so ...
"Culture of Human Embryonic Stem Cells (hESC)". National Institutes of Health. Vaadatud 2013-10-21. ... Lindvall O (2003). "Stem cells for cell therapy in Parkinson's disease". Pharmacol Res 47 (4): 279-87. PMID 12644384. ... "Researchers find new method for turning adult cells into stem cells". Amherst Daily News. Canadian Press. 2009-01-03. Vaadatud ... California Institute for Regenerative Medicine, Tumor Cells Become Drug Resistant by Reverting to a Stem Cell-Like State, New ...
In his speech he used words such as "cell" and "metabolism" in relation to urban design. The Metabolist movement grew out of ... Pritzker Prize, RIBA Gold Medal, AIA Gold Medal, Order of Culture, Praemium Imperiale, Order of Sacred Treasures. ...
Folk medicine consists of the healing practices and ideas of body physiology and health preservation known to some in a culture ... discovered by Paul Ehrlich in 1908 after he observed that bacteria took up toxic dyes that human cells did not. The first major ... Becka J (1980). "The father of medicine, Avicenna, in our science and culture: Abu Ali ibn Sina (980-1037) (Czech title: Otec ... When adopted outside of its traditional culture, traditional medicine is often called alternative medicine.[61] Practices known ...
"The Plant Cell. 3 (11): 1187-1193. doi:10.2307/3869226. JSTOR 3869226. PMC 160085 . PMID 1821764.. ... Andrew F. Smith (October 1994). The tomato in America: early history, culture, and cookery. University of South Carolina. p. 14 ... "Plant Cell Reports. 12: 644-647. doi:10.1007/bf00232816.. CS1 maint: Multiple names: authors list (link) ... "Fruit Cell Wall Proteins Help Fungus Turn Tomatoes From Ripe To Rotten". Science Daily. Jan 31, 2008. Retrieved 29 August 2010. ...
... cell phone strap charms, and Disney character keychains. ... Canadian-American culture. *Canadian culture. *World Showcase. ...
A cultured neuronal network is a cell culture of neurons that is used as a model to study the central nervous system, ... Like most cell cultures, neuron cultures are highly susceptible to infection. They are also susceptible to hyperosmolality from ... They saw bursts throughout the lifetime of the cell cultures, beginning at 4-7 days in vitro (DIV) and continuing until culture ... Cultured networks on traditional MEAs are flat, single-layer sheets of cells with connectivity only two dimensions. Most in ...
Biotech will continue to drive demand for automated cell culture systems market; what other factors will create opportunities ... Automated Cell Culture Systems Market. 2020 Analysis and Review: Automated Cell Culture Systems Market by Cell Culture - Finite ... Automated Cell Culture Systems Market Outlook & Key Findings. *Automated cell culture systems market is expected to witness a ... Automated Cell Culture Systems Market: Key Players. The automated cell culture systems market players are focusing on offering ...
Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... 14:10 PANEL DISCUSSION: Will 3D Cell Culture replace animal models?. * Advantages and disadvantages of 3D Cell Culture vs ... He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell ...
A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon.. Merolli A1, Mao Y1, Voronin ... Schwann cell wrapping.. Top left: a schematic drawing of a Schwann cell wrapping around an axon (black) in transverse section ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ...
Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly by macrophages. Our cell ... Cell culture study * 1. Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly ... 2. Cell Culture StudiesCell Culture Studies We have Comparison of the 3 different SPIOs in the cell culture, and their effect ... Our cell culture studies showed a transient elevation in free radical of macrophages with a rapid return to the baseline. -Next ...
CulturedTumor Cells, CulturedCell Line, TumorA549 CellsCaco-2 CellsHCT116 CellsHeLa Cells +Hep G2 CellsHL-60 CellsHT29 Cells ... Jurkat CellsK562 CellsPC12 CellsTHP-1 CellsU937 CellsEmbryonal Carcinoma Stem Cells ... Tumor Cells, Cultured. Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can ... All MeSH CategoriesAnatomy CategoryCellsCells, ... be propagated in cell culture indefinitely.. Year introduced: ...
3D Cell Culture. 3D Cell Culture. Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ... Cell Culture. Cell Culture. Create a more in vivo-like cell culture to advance your discoveries right from the start. ... 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many ... Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ...
In Epithelial Cell Culture Protocols, a team of well-versed experimenters and cli ... Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. ... Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium ... and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use ...
... a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques f ... New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. ... Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach ... Well-Differentiated Human Airway Epithelial Cell Cultures M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. ...
Cloned calves are grown from cultured cells BMJ 2000; 320 :136 doi:10.1136/bmj.320.7228.136 ... Six cloned calves have been created from cells cultured in vitro for up to three months, confirming previous work showing that ... Cloned calves are grown from cultured cells. BMJ 2000; 320 doi: (Published 15 January ... out specific genes and replacing them with other ones-because this requires prolonged culture of nuclear donor cells. ...
Cell Culture Flow Systems. Lonza For info: 800-638-8174 ... The system consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over ... Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve ... including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. Not only is the system ...
... Stem Cell Training course: Human Pluripotent Stem Cells in Culture. 2017-2018 - Course ... basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). The ... Assessment of PSC culture. • Differentiation of human ES cells: Formation of spontanteous embryoid boides (EB) and defined EBs ... Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells.. Practical ...
Culturing Nerve Cells, Second Edition Gary Banker and Kimberly Goslin ...
... buffer Cancer cell culture cell density cell growth cell lines cell numbers cell population cell suspension cell type ... culture.html?id=g5xqAAAAMAAJ&utm_source=gb-gplus-shareAnimal cell culture. ... Cell culture. Language Arts & Disciplines / Linguistics / General. Medical / Genetics. Science / Life Sciences / Cytology. ... Animal Cell Culture: A Manual of Basic Techniques. Tajamul Islam Shah. Limited preview - 2018. ...
The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor ... ... Dedicated culture rooms. GE S110.01, SP 550.04. SP 430.01 (Whiteway).. Incubation Shakers. GE S110.05, GE S110.09, GE 230.00, ... Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated ...
Cells, cultured synonyms, Cells, cultured pronunciation, Cells, cultured translation, English dictionary definition of Cells, ... cultured. n. 1. The technique or process of growing bacterial or fungal cells or cells derived from tissues of living organisms ... cell culture. (redirected from Cells, cultured). Also found in: Medical, Encyclopedia. cell culture. n.. 1. The technique or ... Cells, cultured - definition of Cells, cultured by The Free Dictionary ...
... and CMOS to provide new content on process development for cell-culture specific scientists. ... Cell Culture World Congress USA connects pharma, biotech, ... The Cell Culture Dish - Media Partner. The Cell Culture Dish is ... Cell Culture World Congress USA 2017. 23 - 24 May. Hilton San Diego Resort and Spa, San Diego ...
... in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability ... We describe a method of growing an animal cell in a culture medium, ... Specific examples of cells include CHO cells, HeLa cells, HEK cells, HEK 293 cells, COS1 cells, embryonic stem cells, African ... Animal Cell The improved cell culture medium is suitable for the culture of any cell or colony or cell line preferably of ...
KudoZ) English to Spanish translation of early-passage primary human cells cultured [cell culturing - Biology (-tech,-chem, ... Medical - Biology (-tech,-chem,micro-) / cell culturing. English term or phrase: early-passage primary human cells cultured. ... Containing early-passage primary human cells cultured +/- stimulus combinations. -Concentrations covered reported clinical ... BIOMAP systems: Model complex signaling networks in primary human cell types to validate compounds and targets, identify ...
The Maxwell RSC Cultured Cells DNA Kit is used with the Maxwell RSC Instrument to provide a simple method for efficient, ... automated extraction of DNA from up to 5 × 10^6 mammalian tissue culture cells and 2 × 10^9 bacterial cells. ... Automated Benchtop DNA Purification from Mammalian and Bacterial Cultured Cells The Maxwell® RSC Cultured Cells DNA Kit is used ... Purification of DNA from 3D cell cultures using the Maxwell® RSC Cultured Cells DNA Kit ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Features a superior cell culture surface with demonstrated performance that elevates stem cell and cell culture research. ... Thermo Scientific™ BioLite Cell Culture Treated Dishes Perform general cell culture procedures more economically with Thermo ... Corning™ TC-Treated Culture Dishes Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. ...
... , Date: 3 Sep 93 05:03:13 GMT , I know that plant cell and tissue culture has been around for long time. , , ... How is it that high value products such as vanilla, taxol, quinine etc , are not widely produced by plant cell culture ? ... Plant cell culture. SPLUHAR at CROP.UOGUELPH.CA SPLUHAR at CROP.UOGUELPH.CA Sun Sep 5 23:19:15 EST 1993 *Previous message: ... An example of such unstable types of cells is Alfalfa suspension cells, which frequently loose chromosomes and double others, ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and ... Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used ... Used for culture of variety of cell types under low serum conditions. Corning™ DMEM/Ham's F-12 50/50 Mix, Powder ensures ...
Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). ... This webinar will introduce basic concepts of cell cultur... ... Cell culture is a fundamental technique for a variety of ... Cell Culture Fundamentals. Cell culture is a fundamental technique for a variety of applications. Cells are cultured in ... Introduction to cell culture. •Critical factors for successful cell culture. •Cell culture tips and techniques. Presenter Bio: ...
... primary cultures, and established cell lines for basic research, drug discovery, and manufacturing. ... Comprehensive cell culture workflow tools and advanced cell culture solutions from cell culture media to sera, supplements, ... Our cell culture workflow portfolio, advanced cell culture solutions, cell counting and imaging equipment, cultureware, ... Cell culture is fundamental in life science research and development. Whether your goal is to establish relevant cell models to ...
I am trying to do primary cell cultures of brain cappilary endothelial ,cells (BBB) and aortic endothelial cells (control). I ... Primary cell cultures. Susan Hubchak hubchak at Fri Jan 27 20:37:53 EST 1995 *Previous message: Primary cell ... BTW, what kind of aortic cells? Bovine? They are easy to isolate and culture. Iwould think there would be plenty of references ... Is there anyone out there who is expert in these sorts of cultures and has ,the time to email me as an advisor on these ...
... cell culture. Includes techniques and descriptions of scaffold and scaffold-free methods matrices for the culture of complex in ... Cell Culture & Cell Culture Analysis. Cell Culture by Technique. 3D Cell Culture ... 3D Cell Culture Tools and Techniques. 3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell ... Stem Cell Culture. An overview of different stem cell types, stem cell culture basics and stem cell applications in basic ...
... including how the heart functions and how cells that create heart tissue behave. Scientists studying these changes on the ...
It cannot regenerate itself, because it is formed by a layer of cells that is lost by the time the tooth appears in the mouth. ... the feeder-layer cells are known as the 3T3-J2 cell line). This crucial step boosts the number of dental epithelial cells ... the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of ... H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 ...
Cell Culture Media and Reagents. Cells from the adult RPE cell line ARPE-19 were obtained from the American Type Culture ... Cell Culture. Adult human retinal pigment epithelial (ARPE-19) cells were routinely cultured in 95% air and 5% CO2 at 37°C in ... Viability of Cultured ARPE-19 Cells following Storage. To study the impact of different temperatures on RPE cell survival, cell ... In the current study, ARPE-19 cells were cultured on glass or plastic culture dishes, reducing the culture variables to a ...
Phthalate ester toxicity in human cell cultures.. Informaci n bibliogr fica. Feb. 1975, Vol.31, No.2, p.283-289. Illus. 21 ref. ... were found to cause significant growth inhibition in cultures of the human diploid cell strain WI-38. The ID50 (dose causing 50 ...
  • New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. (
  • If additional in-house or send-out testing on cultured cells (fibroblasts) is desired, please clearly indicate the desired testing on the requisition form and call Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate testing. (
  • Here, we review techniques and principles involved in preparation and culturing of Big Blue ® mouse embryonic fibroblasts, treatment in vitro with chemical/physical agent(s) of interest, determination of the cII mutant frequency by the λ Select cII assay and establishment of the mutation spectrum by DNA sequencing. (
  • In the 1960s, biologists found that normal human fibroblasts, cells derived from connective tissue , had a predictable limit in their ability to proliferate in culture. (
  • We conclude that lyso-PC can induce growth factor gene expression in cultured endothelial cells and thus may contribute to the migration and proliferation of smooth muscle cells and fibroblasts in various response-to-injury settings in vivo. (
  • Yet with the rise of promising new opportunities within such fields of study as cancer research and stem cell differentiation, using 2D models to obtain useful information about in vivo biological processes has created challenges. (
  • 3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro. (
  • 3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro. (
  • In basic research, 3D cell culture is being used to establish models of disease and unravel the mysteries of differentiation. (
  • With the proper combinations of growth and differentiation factors, mouse ES ( 22 , 23 ) and EG (S.W., unpublished results) cultures can generate cells of the hematopoietic lineage and cardiomyocytes ( 24 , 25 ). (
  • For over 30 years, Corning Matrigel matrix has been widely used in a variety of applications such as general cell culture, in vivo studies, hESC or organoid cultures and differentiation. (
  • Oxygen-sensing transcription systems, such as hypoxia-inducible factors, play an important role in regulating the differentiation of stem cells ( 3 ), as does the distribution of diffusing growth factors. (
  • However, the complexity, variability, and ill-defined nature of these interactions make it difficult to understand the proliferation, differentiation, and migration of cells embedded within these natural gels. (
  • As SCs can only accommodate the differentiation of a finite number of germ cells [ 7 ], a deregulation in SCs apoptosis could affect spermatogenesis, resulting in a lower fertility or even in infertility. (
  • and growth, differentiation and organogenesis in plant tissue and organ cultures. (
  • We are interested in understanding and elucidating transcriptional and/or translational events involved in gene regulation as well as unraveling the genetic, molecular and physiological determinism of growth and differentiation of plant cells, tissues and organs cultured in vitro. (
  • In Jul 2020, Incucyte Live-Cell Analysis System and Incucyte 3D Multi-Tumor Spheroid Assays were used for evaluating 3D spheroids' development with either non-invasive reagents or label-free in real time. (
  • She has worked with xenograft models and many cancer cell lines, and she has expertise in in vitro cell based functional assays and in vivo experiments. (
  • Whether your goal is to establish relevant cell models to probe complex biology, establish drug discovery and drug development assays, or produce recombinant proteins or therapeutics, we have everything you need to advance your science. (
  • Sophisticated models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays. (
  • In addition to spheroids, collagen-based scaffolds that encourage cell aggregation into tumoroids have been used for immunotherapy applications such as natural killer cell cytotoxicity assays. (
  • Physiological relevance is a key parameter to improve predictivity of cell-based assays in drug discovery and toxicity testing for compound. (
  • In culture assays it was possible to test human and sheep agent strain combinations that could not be discriminated in animals. (
  • The µ-Dish 35-mm Quad from Ibidi GmbH is a four-compartment cell culture dish that can be used for simultaneous assays, guaranteeing high optical quality. (
  • Mutation assays in cultured cells of transgenic rodents constitute, in many ways, viable alternatives to in vivo mutagenicity experiments in the corresponding animals. (
  • Nuclear runoff assays documented an increased transcriptional rate for the HB-EGF gene in lyso-PC-treated cells. (
  • This is a hands-on, basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). (
  • Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (
  • To address these limitations, our guest presenter and her team have established a unique model system whereby they can retro-engineer patient-specific GBMs using patient-derived glioma stem cells (GSCs) and human embryonic stem cell (hESC)-derived cerebral organoids. (
  • A pioneering new study led by Florida State University biologists could fundamentally change our understanding of how embryonic stem cells differentiate into specific cell types. (
  • Science Daily reports that for the first time, human embryonic stem cells have been cultured under chemically controlled conditions without the use of animal substances, which is essential for future clinical uses. (
  • Now, for the first time, we can produce large quantities of human embryonic stem cells in an environment that is completely chemically defined," says professor Karl Tryggvason, who led the study at Sweden's Karolinska Institutet. (
  • IPsC are when they take cells from your own body and make them revert back to a similar state to embryonic stem cells, to where they can then be turned into any cell type you want (the advantage there being they're your cells so you wouldn't get tissue rejection like you would with embryonic stem cells. (
  • Over a period of 7-21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. (
  • Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81) that have been used routinely to characterize embryonic stem and EG cells. (
  • Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. (
  • Pluripotent stem cells have been derived from two embryonic sources. (
  • Embryonic stem (ES) cells are derived from the inner cell mass of preimplantation embryos ( 1 , 2 ), and embryonic germ (EG) cells are derived from primordial germ cells (PGCs) ( 3 , 4 ). (
  • Other important characteristics include growth as multicellular colonies, normal and stable karyotypes, the ability to be continuously passaged, and the capability to differentiate into cells derived from all three embryonic germ layers. (
  • This property alone may not be a definitive test of stem cell pluripotency, as it has been demonstrated that rat and mouse visceral (yolk sac) endoderm are capable of forming highly differentiated teratomas containing cells of all three embryonic germ layers ( 29 , 30 ). (
  • While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. (
  • Via homologous recombination in embryonic stem cells (ES cells), it is possible to generate a specific genetic modification at a specific location in the genome. (
  • Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. (
  • 2. The method of claim 1 , wherein the human pluripotent stem cells are embryonic stem cells. (
  • The invention relates to stem cells (SC) in particularly to methods and systems for handling human embryonic stem cells (hESC). (
  • Embryonic stem cell lines derived from human blastocysts. (
  • Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. (
  • Feeder-free growth of undifferentiated human embryonic stem cells. (
  • Embryonic stem cells differentiate more efficiently to blood-forming stem cells when cultured in three-dimensional scaffolds compared to cells cultured in two dimensions ( 2 ). (
  • At the same time, he was propagating und publicizing a new image of life that could be understood as a literal embodiment of durée in the sense of philosopher Henri Bergson: in 1912, Carrel placed tissue from an embryonic chicken heart in a specifically designed flask and maintained this living culture for over 20 years. (
  • In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (
  • Cells grown in vitro from neoplastic tissue. (
  • 2. A culture of cells grown by this technique or process. (
  • When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (
  • Interestingly, spheroids derived from stem cells grown in 3D models show improved "stemness," that is, characteristics that may lead to increased efficacy in regenerative medicine. (
  • The process involves taking tissue from a live animal and extracting stem cells which are then grown into muscle fibres in a bioreactor. (
  • Marianne Ellis is a Biochemical Engineer at Bath University, UK and her team has taken cells grown and matured in a bioreactor and then combined them with other ingredients to make products such as burgers, sausages and meat balls. (
  • EBs from mouse pluripotent stem cells grown on feeder layers or in suspension may contain a variety of cell types. (
  • Studies were performed in both rabbit and rat renal PT cells grown in primary culture, as previously described. (
  • When cells have grown (by dividing) to cover the flasks' surface, the process of enzymic dissociation can be repeated and the cells replanted to additional flasks. (
  • Skin cells have been grown in the laboratory since the mid 1970s, but until now there has not been a significant scientifically controlled study. (
  • The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (
  • Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (
  • Growing incidences of chronic diseases have resulted in upswing in research related to cell biology and cytology. (
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell research. (
  • Scientific advances in cancer cell biology are changing the paradigm in oncology research. (
  • Current preclinical GBM models are limited by the lack of a "normal" human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. (
  • This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians. (
  • Classical cell biology teaches that exocytosis causes the membrane of exocytic vesicles to disperse into the cell surface and that a cell must later retrieve by molecular sorting whatever membrane components it wishes to keep inside. (
  • The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (
  • Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (
  • The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (
  • We propose to apply a systems biology approach to achieve a computational representation of tissue-level physiological responses by integrating empirical data derived from organotypic culture systems with computational models of intracellular pathways to better predict human responses. (
  • 3D cell culture enables cells to grow and interact with their surroundings in all three dimensions, better mimicking the in-vivo conditions in which the cells are naturally present. (
  • Create a more in vivo-like cell culture to advance your discoveries right from the start. (
  • As research demands have become increasingly complex, we have been there every step of the way, creating 3D cell culture solutions that allow you to produce optimal environments for growing cells that exhibit in-vivo like behaviors and functionality. (
  • Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve cellular phenotypes and human in vivo relevance of in vitro cell culture models. (
  • Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (
  • Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (
  • Though these manufactured supports are less like the in vivo ECM, they may enhance reproducibility and facilitate cell retrieval from the culture. (
  • Advanced 3D cell systems allow researchers a hybrid between the accessibility of classical 2D cell culture techniques and the biological relevance of in vivo animal models, with fewer ethical concerns. (
  • Sartorius Stedim Biotech, a leading international supplier for the biopharmaceutical industry today announced the launch of the BIOSTAT RM TX single-use bioreactor, a new wave mixed system developed specifically for closed, automated expansion of consistent quality cell products such as ex vivo cellular immunotherapies. (
  • It is becoming increasingly clear that these models mimic cells in vivo at a greater capacity than traditional cell culture. (
  • Until now it has not been possible to establish progressively growing cultures of these cells in vitro , and cell lines have only been obtained after teratocarcinoma formation in vivo . (
  • These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo . (
  • Culture systems that better mimic the biological milieu are needed to bridge the gap between conventional cultures and complex native in vivo environments. (
  • The pluripotency of ES and EG cells can be demonstrated in vitro and in vivo . (
  • Perhaps the most definitive in vivo test of developmental potential would be a demonstrated contribution to all cell lineages in a chimeric animal, but this test is not practical or possible for all species and cannot be done with human cells. (
  • It uses a novel method to create high-density collagen scaffolds with cells embedded, enabling improved cellular interactions that more closely resemble an in vivo environment. (
  • Cells can be isolated from tissues for ex vivo culture in several ways. (
  • Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers over conventional cell culture methods. (
  • In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. (
  • In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (
  • 3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (
  • The establishment of future retinal pigment epithelium (RPE) replacement therapy is partly dependent on the availability of tissue-engineered RPE cells, which may be enhanced by the development of suitable storage methods for RPE. (
  • With the advancement of RPE cell replacement therapy, and with 20-25 million known sufferers from AMD worldwide [ 17 ], a great need for improved storage methods for cultured RPE is likely to emerge. (
  • b ) Culturing cells on 2D hydrogel films has some of the same disadvantages as conventional methods but permits user-defined control of the substrate stiffness and adhesive ligand presentation. (
  • Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. (
  • Provided are systems and methods for providing human cell cultures. (
  • Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development. (
  • If you are adopting the next-generation technologies to improve your daily lives such as smart phones, then why stick to the old cell culture methods and compromise results in the lab? (
  • After the Second World War, methods of culturing tissue were further improved and provided the basis for a genuine mass (re)production of cells. (
  • Accordingly, most culture methods require sterile conditions. (
  • Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. (
  • GBI Research, the leading business intelligence provider, has released its latest research report, Manufacturing of Vaccines - Cell Culture Technology Gradually Replacing Egg-Based Manufacturing, which provides insights into the production methods of vaccines and into vaccine production capacity. (
  • Previous experience in either microengineering systems or cell culture methods would be advantageous. (
  • Protocols in this series are examples of methods for general cell culture and have not been rigorously validated and optimized by QIAGEN. (
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (
  • This guide contains general technical information for working with animal cells in culture, including media, subculturing, cryopreservation and contamination. (
  • This enhances the ability for scientists to study basic biological mechanisms such as cell number monitoring, cell viability, proliferation and morphology. (
  • We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. (
  • 18. A method according to claim 1, in which cell growth or cell viability is enhanced by at least 50% compared to growth in a cell culture medium without an elevated concentration of thymidine family member. (
  • 19. A method according to claim 1, in which apoptosis of the animal cell in the cell culture medium is reduced to enhance cell viability. (
  • Support non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins with Thermo Scientific™ Nunc™ Dishes with UpCell™ Surface. (
  • The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers high content format for inducing tumor spheroid formation and quantitating cell viability within the spheroids by absorbance using MTT. (
  • The 3-D Spheroid Fluorometric Proliferation/Viability Assay offers a high content format for inducing tumor spheroid formation and quantitating cell viability within the spheroids using resazurin fluorescent detection. (
  • This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (
  • Out of nine temperatures tested between 4°C and 37°C, storage at 12°C, 16°C, and 20°C was optimal for maintenance of RPE cell viability, morphology, and phenotype. (
  • Based on previous publications on storage of cultured epithelial cells [ 20 - 23 ], we hypothesize that differences in storage temperature between 4°C and 37°C affect the viability, morphology, and phenotype of cultured RPE cells. (
  • I personally think that the massive interest in cultured meat brought a lot of interesting scientific ideas to market, but some of them lack a path for commercial viability. (
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (
  • 1. The technique or process of growing bacterial or fungal cells or cells derived from tissues of living organisms in a culture medium. (
  • Bioinks selection guide for 3D bioprinting of cells for tissues engineering applications. (
  • The cell-scaffold approach is based on tissue-engineering technology developed at the Forsyth Institute (MA) and was applied by one of the Tokyo researchers to produce enamel-like tissues in 2002. (
  • The field of biomaterials continues to advance the introduction of such complexity into cell culture systems, providing ways to control mechanical, compositional, and structural cues and thus more accurately represent features of native tissues 2 . (
  • However, hydrogels-water-swollen networks of polymers-have emerged as the most promising option for cell culture ( Fig. 2 ) since they mimic salient elements of native extracellular matrices (ECMs), have mechanics similar to those of many soft tissues, and can support cell adhesion and protein sequestration 3 . (
  • Although native tissues possess subtleties that still elude 3D culture systems, scientists are making progress. (
  • The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. (
  • The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. (
  • The selection is a vital source of data for readers interested in the culture of cells and tissues. (
  • Rapidly isolates primary cells to consistently obtain a uniform single-cell suspension from tissues. (
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (
  • Used as basal medium for supporting the growth of many different mammalian cells. (
  • This webinar will introduce basic concepts of cell culture and provide tips on how to succeed in culturing mammalian cells. (
  • The book is written for Master Students and PhD Candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. (
  • A majority of these complex molecules are produced by genetically modified mammalian cells in large-scale cell cultures. (
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (
  • For example, human breast epithelial cells proliferate abnormally (like tumor cells) when cultured as a two-dimensional monolayer, but display normal cell growth behavior and form structures typical of breast tissue when cultured in three-dimensional membranes that resemble their native environment ( 1 ). (
  • Oxidative stress resulted in a profound influence on advancing the senescence (functional deterioration) of these cells and inhibiting their proliferation. (
  • This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. (
  • This characteristic can be used for the indirect quantification of cell death and to determine differences in proliferation upon stimulation with death-inducing agents. (
  • To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. (
  • To support the proliferation and plating efficiency of renal epithelial cells (e.g. (
  • Interestingly, cells engineered to express more telomerase retained telomeres and the ability for extended proliferation. (
  • In May 2020, HORIBA Medical entered into collaboration with CellaVision regarding automated digital cell morphology solution. (
  • b ) Mouse MSCs cultured in 3D alginate hydrogels display rounded morphology regardless of substrate stiffness. (
  • Conventional 2D culture conditions are highly limited as native tissue morphology and cell composition is not properly reflected. (
  • Another difficulty with some cultured cells is their tendency to change their morphology , functions, or the range of genes they express. (
  • It will also provide managers with insights into the intricacies of cell culture and the necessary laboratory infrastructure requirements. (
  • The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor laboratory areas. (
  • The idea of developing a laboratory instrument that handles and sorts individual, viable cells has been realized by researchers in the Laboratory for MEMS Applications in the Department of Microsystems Engineering at the University of Freiburg. (
  • In a search for alternative sources of meat and in response to concerns over meat consumption, animal welfare and greenhouse gas emissions, scientists have developed the ability to grow meat from animal cells in a laboratory. (
  • Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines. (
  • Cell culture disposables for cell cultivation and cell monitoring in laboratory scale. (
  • With her book Culturing Life , Hannah Landecker contributes an important chapter to the recent history of these laboratory fractals. (
  • Cell culture describes the laboratory growth of cells derived from plants or animals. (
  • The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (
  • Animal cell culture is an important laboratory technique in the biological and medical sciences. (
  • This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. (
  • Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap offer a convenient solution to prepare laboratory samples. (
  • 1998) Cells: a Laboratory Manual. (
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (
  • Delegates will leave the module with practical knowledge to propagate, maintain and characterize feeder and feeder-free stem cell lines. (
  • Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells. (
  • Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. (
  • This group has recently shown that epithelial cells extracted from the developing teeth of 6-month-old pigs continue to proliferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known as the 3T3-J2 cell line). (
  • The idea for the culturing technique originates from 1975, when Dr. J.G. Rheinwald and Dr. H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were gifted by Dr. Green). (
  • culturing them required feeder cells which the article describes why that's bad, and 3. (
  • With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. (
  • 4. The method of claim 1 , wherein the human umbilical cord feeder cells consist of 100% human umbilical cord fibroblast feeder cells. (
  • 7. The method of claim 6 , wherein the human umbilical cord feeder cells consists essentially of the human umbilical cord fibroblast feeder cells. (
  • When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (
  • Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (
  • Incucyte S3 live-cell analysis system was devised lately for defining spontaneous neuronal activity as well as connectivity from hiPSC (human-induced pluripotent stem cells)-derived neurons after transducing GECI (genetically encoded calcium indicator). (
  • The role of patterned electrical impulses has been investigated in the literature using co-cultures of neurons and myelinating cells. (
  • The co-culturing method, however, prevents the uncoupling of the direct effect of patterned electrical impulses on myelinating cells from the indirect effect mediated by neurons. (
  • In addition, mouse ES cells have been used to generate in vitro cultures of neurons ( 26 ), skeletal muscle ( 27 ), and vascular endothelial cells ( 28 ). (
  • Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. (
  • Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. (
  • She has extensive experience in a variety of cell culture techniques including isolation and culture of primary patient cells. (
  • Later: Freezing and DNA isolation of part of the ES cell clones. (
  • This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. (
  • Finally, many standard techniques for analyzing proteins and protein distributions are more difficult to perform, because they require isolation of the cells from the matrix. (
  • Join us in February 2019, as we look at the latest developments in the 3D Cell Culture field. (
  • In the study being reported today, the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of the tooth (dental mesenchymal cells). (
  • The scaffolds were then transferred into the abdominal cavities of rats, where conditions were favorable for the cells in the scaffolds to interact and develop. (
  • Researchers globally are realizing the potential of 3D cell culture for various applications, including testing and discovering new drugs to treat cancer, organ-on-chip models to study the human physiology in an organ specific context, and 3D cell printing to produce organ models. (
  • Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (
  • Today, during the 85thth General Session of the International Association for Dental Research, a team of researchers from the Institute of Medical Science, the University of Tokyo (Japan), reports on a new technique for culturing cells that have the capacity to produce enamel. (
  • Researchers have seen that spheroids display enhanced anti-inflammatory, tissue regenerative, and reparative responses, as well as better post-transplant survival of mesenchymal stem cells. (
  • UPM launches GrowDex-T - a new hydrogel offering researchers exceptional imaging properties for visualization of cells in 3D. (
  • Although most scientists concede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recapitulating native cellular milieus, there is currently a knowledge barrier for many researchers in regard to the application of hydrogels for cell culture. (
  • Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study. (
  • With the increasing incidence of neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, and Parkinson's disease, researchers are testing 3D cellular models that could be superior to animal studies because the cells are derived from humans instead of rodents. (
  • Many researchers routinely use media containing 10 to 20% FBS for growing the cells, but these levels may be higher than cells require. (
  • Researchers are now bridging the gap between natural and synthetic gels by combining well-characterized synthetic materials with biomimetic cues to support physiologically relevant cell-gel interactions (see the figure). (
  • Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. (
  • This comprises advanced 3D cell culture models for efficacy and safety studies, enabling technologies to create and analyze biological reactions as well as their successful implementation in compound de-risking. (
  • The company's exVive3D™ human liver tissue model is constructed from three basic cellular elements: primary hepatocytes, primary endothelial cells, and primary stellate cells. (
  • Two key aspects of this process are initial endothelial cell (EC) dysfunction and subsequent phenotypic change and accumulation of smooth muscle cells (SMCs), with inherent disruption in local cell-cell communication. (
  • Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. (
  • In this study, we have found that lyso-PC increased steady state mRNA levels for two smooth muscle/fibroblast-directed growth factors, the A and B chains of PDGF and heparin-binding EGF-like protein (HB-EGF), in cultured human endothelial cells. (
  • Lyso-PC did not upregulate the expression of certain other inducible endothelial genes, including E-selectin, IL-8, or monocyte chemoattractant protein-1 in the same cells, in contrast to the coordinate pattern of activation typically observed with other stimuli, such as TNF alpha, bacterial endotoxin, or PMA. (
  • The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange. (
  • Adherent cells detach from cell culture plates during cell death. (
  • For adherent cells, it is important to produce a single-cell suspension after trypsinizing. (
  • Applications such as transfection or immunofluorescence staining or live-cell imaging can be conveniently performed. (
  • It is often necessary to count cells, for example, when plating cells for transfection experiments. (
  • From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (
  • Rely on innovative vessels from Corning for quality and consistency in your cell culture applications. (
  • It is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. (
  • Corning™ Untreated 245mm Square Low Profile BioAssay Dishes provide a superior option for applications where cell attachment is not desired. (
  • Using Nunc Glass Bottom Dishes in your research applications gives you the flexibility to convert your culture device into an imaging device. (
  • Gibco™ DMEM, Powder, High Glucose, No Sodium Bicarbonate Buffer is used in cell culture processing applications. (
  • Cell culture is a fundamental technique for a variety of applications. (
  • And in therapeutics, 3D cell culture promises to realize several exciting applications, from patient-specific drug-efficacy screening to the manufacturing of bioprinted organs. (
  • GEN: Are 3D cell culture models as strongly focused as ever on drug safety testing, or are they finding new applications? (
  • We would argue that 3D cell culture models have been used for many years in basic research and disease modeling, notably in cancer research-this was, after all, one of the original applications of Corning® Matrigel®, a naturally occurring extracellular matrix for us in 3D cell culture. (
  • Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. (
  • Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (
  • Fortunately, bioprinters are becoming commonplace, and the applications that they enable-such as 3D cell cultures-are starting to go mainstream. (
  • Click here to register for free and to view the entire webinar: Since 2008 Kaiser Optical has worked closely with industry leading bioprocessing companies to develop a Raman solution enabled to deliver in situ, real-time measurement to a host of bioprocessing applications including but not limited to cell culture, fermentation, and purification. (
  • Since our first symposium in 2009, the 3D Cell Culture conference series has developed into a 'place to be' for experts active in this field, and will highlight successful applications and newest developments. (
  • Success stories of 3D culture applications will be presented as well as newest technologies and innovations. (
  • Relevant disease models, applications of 3D models in clinic and industry, predictive cell models for compound characterization and enabling technologies will be covered. (
  • This textbook provides an overview on current cell culture techniques, conditions and applications, it specifically focuses on human cell culture. (
  • Naturally based" hydrogels such as Matrigel (made of native extracellular matrix proteins) and collagen (the major extracellular matrix protein) have been explored extensively for applications in three-dimensional tissue culture and regenerative medicine. (
  • In this thesis, the macroscopic kinetic model type is the starting point, but the goal is to capture a variety of culture conditions, as will be necessary for future applications in process optimization. (
  • Corning™ Stripette™ Individually Wrapped Plastic or Paper/Plastic Disposable Polystyrene Serological Pipets are ideal for sterile tissue culture applications, especially while wearing latex gloves, as the wrap reduces static cling. (
  • These disposable, space saving containers are specifically designed for optimal plant cell culture applications. (
  • The 'Recombinant Cell Culture Supplements' market can be divided based on product types and It's sub-type, major applications and Third Party usage area, and important regions. (
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (
  • Now that dental epithelial cells can be propagated in culture, the next step will be to achieve the same success with their partners in tooth formation, the dental mesenchymal cells. (
  • Human mesenchymal stem cells (MSCs) cultured on increasingly stiff 2D substrates display increasing spread area. (
  • There have been some breakthroughs on 1, last I heard a group had shown you can just culture with modified proteins to induce pluripotency. (
  • Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses. (
  • One simple method to detect maintained adherence of cells is the staining of attached cells with crystal violet dye, which binds to proteins and DNA. (
  • Physiological processes are guided by interactions between cells and their extracellular matrix, the proteins and polysaccacharides that cells secrete into their environment to support tissue structure and survival. (
  • The advantages of synthetic gels include their consistent composition and predictable manipulation of properties, but they lack functional sites to interact with soluble or cell-surface proteins. (
  • The second approach explored how the kinetics of amperometric spikes changed in intact cells while stimulus patterns varied ( 8 ), or in permeabilized cells while they overexpressed proteins involved in exo- and endocytosis ( 9 ). (
  • d) repeating steps (b) and (c) as necessary to produce extracellular matrix proteins in the three dimensional stromal culture. (
  • Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are possible in insect cells whereas this mechanism is not present in prokaryotic systems. (
  • This section provides useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). (
  • Part V: Counting cells Welcome to the fifth of a series of articles providing useful hints for culturing animal cells. (
  • If tissue from products of conception (POC) is submitted and molecular genetic testing is requested on cultured cells, then submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (
  • cell cultivation via incubators, incubation and benchtop shakers and cell harvest via homogenisers and centrifuges with varoius volumes as well as PCR-based tests for mycoplasma contamination control. (
  • If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (
  • The limitations of cell culture include the finite doubling potential of most normal cells, the possibilities for unexpected infection with viruses or microorganisms, or even cross-contamination with other cell types. (
  • This eliminates the worry of cross-species contamination when using FBS with human cells. (
  • BD Biosciences: Diverse set of tools for stem cell research. (
  • Furthermore, disease research areas are expanding to include neurology, stem cell research, cell therapy , and (potentially) tissue engineering. (
  • As Paul Rabinow does in Making PCR: A Story of Biotechnology (1996), Landecker poses the question of how today's cell manipulations-from cloning techniques to stem cell research and reproductive medicine-have altered our understanding and experience of life. (
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (
  • 21. A method according to claim 1, in which the animal cell expresses a heterologous or recombinant protein. (
  • A team of scientists led by Prof. Antonella Consiglio from the IDIBELL and the University of Barcelona, and Prof. Angel Raya from the Center of Regenerative Medicine of Barcelona have discovered that defective versions of human brain cells called astrocytes are linked to the buildup of a toxic protein that is one the hallmarks of Parkinson's disease. (
  • This group has been working on showing this goes on in human stem cells for at least 2 years, who knows how long it took them to find this out in mice, or narrow down this one specific protein. (
  • I would guess that the next step, maybe one they're already working on, is to show that induced pluripotent stem cells can be cultured on this same protein. (
  • Human MSCs spread more with increasing stiffness, but cells on laminin-coated hydrogels are smaller than those on other ECM protein coatings. (
  • Ellis also argues that cultured meat can bring benefits for food security, in terms of protein, and significant environmental sustainability benefits due to relatively lower greenhouse gas emissions, water use and land use, and less energy, compared to traditional meat production. (
  • 4 Since angiotensinogen protein, mRNA, and ACE have been identified in the PT cell, 5 6 7 8 renin had been, until recently, the only missing component for a complete RAS in the PT. (
  • SCs cultured in insulin deprivation demonstrated a significant decrease on mRNA levels of p53, Bax, caspase-9, and caspase-3 followed by a significant increase of Bax and decrease of caspase-9 protein levels relatively to the control. (
  • Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use. (
  • There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from Bombyx mori, Mamestra brassicae, Spodoptera frugiperda, Trichoplusia ni, and Drosophila melanogaster being of particular interest. (
  • We look forward to meeting you at 3D Cell Culture 2018 in Freiburg! (
  • 2018. "Mutation Analysis in Cultured Cells of Transgenic Rodents. (
  • Page 617 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. (
  • This test is intended for tissue samples that do not have any accompanying cytogenetic test order (e.g. chromosome analysis or microarray analysis) but needs to be cultured for other molecular genetic or biochemical testing (e.g. (
  • It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. (
  • In summary, these results confirm the presence of renin mRNA in cultured proximal tubular cells and suggest that a low-level, constitutive secretion of renin occurs in this system that is decreased by A23187. (
  • Renin secretion from JG cells has been well described: Renin release from these cells is largely regulated by intracellular calcium and cAMP, such that a decline in intracellular calcium or an increase in cAMP stimulates renin secretion in JG cells. (
  • This model type is derived from typical data obtained in the industry, and has been able to simulate cell growth and the uptake/secretion of important metabolites. (
  • The effects of varied availability of amino acids in the culture medium on cell growth, uptake/secretion of metabolites, and product secretion were studied in cell cultures. (
  • We have investigated the hypothesis that fluid secretion by these cells is dependent on cAMP-mediated chloride secretion. (
  • The rate of fluid secretion, cell volume and changes in intracellular Cl- were measured. (
  • These data indicate that cultured ADPKD cells can direct fluid transport in either the absorptive or the secretory direction, and that cAMP stimulates secretion and this secretion is accompanied by a net loss of cell solute. (
  • These data support the thesis that chloride secretion drives fluid secretion by cultured ADPKD cells. (
  • Moreover, development of culture techniques ensuring specificity and specialty growth media, and rising application of high-end technologies to scale up culture production are expected to bolster the market growth over the following decade. (
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (
  • In addition, the third floor culture room is equipped with one lighted incubation shaker and a Coy gloveless anaerobic chamber which was converted into a light chamber (owned by the Martin lab) for the growth of photosynthetic organisms. (
  • The New Brunswick Innova 4430 incubation shakers are used for the growth of microbial cultures in liquid media. (
  • Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. (
  • Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. (
  • Bis(2-ethylhexyl) phthalate - often referred to as di-2-ethylhexyl phthalate (DEHP) - and butyl glycolyl butyl phthalate (BGBP), plasticisers which may be leached into the blood from medical devices with polyvinyl chloride (PVC) parts, were found to cause significant growth inhibition in cultures of the human diploid cell strain WI-38. (
  • Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. (
  • The global market for 3D cell cultures is poised for explosive growth. (
  • Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. (
  • How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth? (
  • Plate different dilutions of your cell line and see were the cut off point is, usually theres a treshold were the cells are suddenly to sparsely plated to give rise to normal growth. (
  • The Ph+ feature avoids any meniscus effect and facilitates homogenous cell growth. (
  • 2. The cell growth apparatus according to claim 1, wherein each compartment comprises opposing gas permeable, liquid impermeable surfaces. (
  • 3. The cell growth apparatus according to claim 1, wherein each compartment comprises a plurality of gas permeable, liquid impermeable surfaces which form the expandable walls of the compartment. (
  • 4. The cell growth apparatus according to claim 1, wherein the gas permeable, liquid impermeable surfaces are selected from the group consisting of polystyrene, polypropylene, polyethylene, polycarbonate, silicone rubber, fluoroethylenepropylene copolymer and a combination thereof. (
  • 6. The cell growth apparatus according to claim 1, wherein the spacer material is selected from the group consisting of a woven mesh and a planar sheet comprising protruding support features. (
  • 7. The cell growth apparatus according to claim 1, further comprising a support structure external to the compartments. (
  • 8. The cell growth apparatus according to claim 7, wherein the support structure comprises a compartment frame perimetrically surrounding each of the compartments for supporting the gas permeable, liquid impermeable surfaces. (
  • 9. The cell growth apparatus according to claim 7, wherein the support structure comprises a top platen and a bottom platen confining the plurality of compartments there-between. (
  • 10. The cell growth apparatus according to claim 7, wherein the support structure is optically transparent. (
  • 11. The cell growth apparatus according to claim 9, wherein the support structure further comprises at least one rod inserted through a supported hole in at least one of the compartments and attached to the top platen and the bottom platen. (
  • 12. The cell growth apparatus according to claim 10, wherein the rod is telescoping to form an expandable support structure. (
  • 13. The cell growth apparatus according to claim 1, wherein the interconnecting passageway comprises ports in the gas permeable, liquid impermeable surface of each compartment. (
  • This protocol describes a quick and reliable screening method that is suitable for the examination of the impact of chemotherapeutics or other compounds on cell survival and growth inhibition. (
  • Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040). (
  • Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040). (
  • Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. (
  • Dispersed cells are then transferred to a suitable growth medium and allowed to attach to the surface of culture flasks. (
  • Cell culture requires careful attention to the growth medium to ensure cells are given all the components they require to grow. (
  • Often the culture medium requires growth factors or hormones to stimulate growth. (
  • Media used to propagate cells are rich in nutrients and, therefore, support growth of a multitude of organisms. (
  • Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (
  • Corning™ Falcon™ Tissue Culture Treated Flasks features vacuum-gas plasma tissue culture treatment to ensure consistent growth surface. (
  • Designed to improve usability while maintaining the same environment for cell growth as previous designs. (
  • Designed to support cell culture growth and biomanufacturing processes. (
  • Resuspend the cells in an appropriate volume of prewarmed growth medium. (
  • The volume and the dry weight of a Tetrahymena cell are determined by the particular medium and the growth temperature. (
  • Large cells are seen in concentrated media and at low growth temperatures resulting in cell volumes up to 17,000 microns 3, whereas starving cells decrease gradually towards 700 microns 3 or even smaller sizes. (
  • Cell growth kinetics, division asymmetry and volume control at division in the marine dinoflagellate. (
  • All plant cell cultures in the PCCL are categorized into three levels of growth, High , Medium and Low . (
  • In the absence of the latter signal, the T cell makes only a partial response and, more importantly, enters an unresponsive state known as clonal anergy in which the T cell is incapable of producing its own growth hormone, interleukin-2, on restimulation. (
  • however, only the white cells are capable of growth in culture. (
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (
  • Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (
  • Individual cultured ADPKD cells were suspended within a polymerized collagen matrix and stimulated to form cysts. (
  • For example, renal organoids derived from pluripotent stem cells have been successfully transplanted under the renal capsules of adult mice. (
  • Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC ® Primary Cell Solutions™ system. (
  • This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. (
  • Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. (
  • Epithelial cells cultured from the renal cysts of patients with autosomal dominant polycystic kidney disease (ADPKD) secrete fluid via a process stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). (
  • The book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover and therefore delivers in a compact and clear way important theoretical as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. (
  • An example of such unstable types of cells is Alfalfa suspension cells, which frequently loose chromosomes and double others, if they are kept growing in culture for a long time. (
  • Day 4: Sub-confluent ES cell colonies are washed twice with PBS and treated with trypsin to obtain a single cell suspension. (
  • see QIAGEN News 2002 No. 3, 20) or by centrifugation at 200 x g for 5 min (suspension cell cultures). (
  • 4. Mix the cell suspension sample thoroughly. (
  • Tip - The cell suspension is drawn under the coverslip and into the chamber by capillary action. (
  • The cell suspension should just fill the chamber. (
  • If the cell density is too high, the cell suspension should be diluted, noting the dilution factor. (
  • If the original cell suspension was diluted for counting, multiply by the dilution factor to obtain the number of cells/ml. (
  • The effects of these agents on renin mRNA were examined in rabbit and rat proximal tubular cells in primary culture with the use of an S 1 nuclease protection assay. (
  • This study, reported in the journal Science, duplicated and extended results of the animal experiments in a neural cell culture assay. (
  • In the first, exocytosis of single granules was detected as step increases in membrane capacitance, an assay of cell surface area. (
  • Furthermore, we highlight representative studies in which the Big Blue ® mouse cell culture model and the λ Select cII assay have been used for mutagenicity testing of diverse carcinogens. (
  • Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, and discover novel 3D cell culture systems, organ-on-chip, 3D imaging of organoids and other technologies in development. (
  • Look to Corning for the right surface for every cell-from biological to synthetic-to provide a cell culture environment that meets your research requirements. (
  • Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated tabletop centrifuge, a basic compound microscope as well as a variety of incubators, water baths and shakers. (
  • Bone marrow contains biological factories, which pump out billions of new blood cells daily. (
  • Her elegantly written and well-documented narrative demonstrates how, within 20th-century biological laboratories, a whole range of vessels and containers were used in order to store, breed, study, and manipulate cells and tissue outside the body of organic [End Page 312] individuals: from hanging-drop preparations in the 1910s to tissue culture flasks in the 1930s and powerful freezers in the 1950s and 1960s. (
  • Culturing Life points to biological research practices in the lab, but at the same time it refers to our contemporary culture that, in more than one way, refers to and relies on developments in the life sciences. (
  • As Landecker shows, however, the rather unexpected adoption of Harrison's technique by the French experimental surgeon Alexis Carrel (from the Rockefeller Institute for Medical Research) crucially contributed to establishing the method of tissue culture in medical and biological contexts. (
  • it's hard to completely differentiate a population of pluripotent cells into one cell type you're trying to get. (
  • And each different cell type seems to differentiate in different ways. (
  • 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many uses. (
  • Due to strict regulatory demands [ 18 , 19 ], the development of a suitable storage method will be essential to enable the transportation of viable cell constructs from centralized laboratories to operating theatres [ 18 ]. (
  • Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (
  • AXT is pleased to announce it has further enhanced its cellular research product portfolio through the addition of stem cell solutions from StemBioSys. (
  • First, even in two-dimensional culture, heterogeneities exist in the cellular microenvironment, and these will only be further exaggerated in three-dimensional gels. (
  • It will enable simultaneous electrical-optical cell monitoring and will be used to characterise communication between cell populations, with modes of cellular communication teased apart by systematically controlling cell-cell interactions. (
  • Cultures of Tetrahymena pyriformis, T. hegewishi and T. malaccensis have been studied with regard to control of cell volume and cellular dry weight. (
  • In all cases the changes in cell volume are paralleled by changes in cellular dry weight. (
  • For this reason, it is exciting to consider the prospect of artificially growing enamel, or even whole teeth, using culturing and transplantation techniques. (
  • With the demonstration of long-term survival of RPE cell transplants both in various animal models and in humans [ 8 - 13 ], transplantation offers the prospect of a single intervention cure. (
  • The transplantation of RPE grafts enables appropriate implantation and orientation of an organized RPE cell layer in the retina [ 5 , 14 , 15 ] and circumvents several of the complications associated with the use of RPE cell suspensions [ 6 , 8 , 15 , 16 ]. (
  • Autologous tissue for transplantation may also come from organoids produced via 3D cell culture. (
  • The cover can be snapped on tight, for long-term culture, or loosely fitted during acclimation to external conditions prior to transplantation. (
  • Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (
  • New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. (
  • The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. (
  • A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. (
  • They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. (
  • It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. (
  • A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. (
  • Although the simplicity of these platforms is attractive, cells cultured in these environments tend to display aberrant behaviors: flattened shape, abnormal polarization, altered response to pharmaceutical reagents, and loss of differentiated phenotype ( Fig. 1 ). (
  • The subdivisions enable up to four parallel, individual experiments in one single dish, which saves time and decreases experimental costs by reducing cell numbers and the amount of reagents needed. (
  • a ) Conventional 2D culture on superphysiologically stiff plastic or glass substrates leads to cells displaying aberrant phenotypes. (
  • Certain plant cell lines can be genetically unstable. (
  • Type 1 diabetes mellitus (T1DM) is characterized by the autoimmune destruction of insulin-producing pancreatic islet beta cells [ 27 , 28 ] in genetically susceptible individuals, resulting in the total lack of insulin. (
  • The lifespan of most cells is genetically determined, but some cell culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (
  • Plant cell, tissue and organ culture. (
  • Plant Cell, Tissue and Organ Culture - incl. (
  • Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. (
  • Here, we introduce hydrogels to those who may be unfamiliar with procedures to culture and study cells with these systems, with a particular focus on commercially available hydrogels. (
  • Introduction: why hydrogels for cell culture? (
  • Figure 1: Cell culture atop 2D hydrogels. (
  • a ) 3D hydrogels can be engineered to present a more realistic microenvironment to cells. (
  • The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. (
  • A major cost component of cell culture is fetal bovine serum (FBS), an often essential and very expensive. (
  • Future Meat does not use animal serum (the liquid part of blood) in its process (some start-ups growing cultured beef, for example, began by using fetal bovine serum, a byproduct of the livestock industry, although they have since claimed to have validated animal-free alternatives). (
  • We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. (
  • Oxidative Stress Induces Senescence in Cultured RPE Cells, Open Neurol. (
  • To create tissue-specific structures and function in vitro, cell models have to be translated into the third dimension. (