The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)

The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen.  (+info)

In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)

Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (6/155046)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Cell Culture ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... A 3D cell culture model showed cell growth similar to that of in vivo than did a monolayer culture, and all three cultures were ...
Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment ... usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass ... dependent cell types. v t e (Articles lacking sources from December 2009, All articles lacking sources, Laboratory equipment, ...
The Sf9 cell line is one of the most commonly used lines in insect cell culture. Drugmand, Jean-Christophe; Schneider, Yves- ... v t e (Cell culture techniques, Biopharmaceuticals, All stub articles, Pharmacology stubs). ... Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are ... The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are ...
... and cell loading would need to be added for the cell culture maintenance. Perfusion in microfluidic cell culture is important ... Gel free 3D cell cultures have been developed to allow cells to grow in either a cell dense environment or an ECM poor ... Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... These reduced cell numbers make studying non-dividing or slow dividing cells (e.g., stem cells) easier than traditional culture ...
Cell culture Cell lines Cell culture assay Hydrogel Madin-Darby Canine Kidney cell line Microphysiometry Fey S, Wrzesinski K ( ... cell-cell/cell-matrix interaction, and/or cell migration, but the available assays are not optimized for 3D cell culturing. ... Lastly, 3D cell cultures have greater stability and longer lifespans than cell cultures in 2D. This means that they are more ... Furthermore, 3D cell cultures provide more accurate depiction of cell polarization, since in 2D, the cells can only be ...
... through the inhibition of cell metabolic pathways. Cell culture evaluations are the precursor to whole animal studies and are a ... A cell culture assay is any method used to assess the cytotoxicity of a material. This refers to the in vitro assessment of a ... Cell culture assays are standardized by ASTM, ISO, and BSI (British Standards Institution.) Biology portal Technology portal ... Löw-Friedrich, I; von Bredow, F; Schoeppe, W (1991). "A cell culture assay for the detection of cardiotoxicity". Journal of ...
... is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture ... More specific terms include organotypic culture, tissue slices and explants. Neuronal primary cell cultures are cells collected ... primary cell cultures did not become widespread until the 2000s. These cultures present several advantages over cell lines, ... In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized ...
The cell culture bioreactor-known commercially as the Rotary Cell Culture System (RCCS) -- boasts several advantages that ... Lab-grown cell cultures tend to be small, flat and two-dimensional, unlike normal cultures. But tissues grown in the RCCS are ... The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. In the ... A closed tubular cylinder forms the system's cell culture chamber, which is filled with a liquid medium in which cells grow on ...
Another type of material that is used for open-microfluidic cell culturing is paper-based microfluidics. Cell culturing on ... such as quorum sensing or co-culturing of several types of cells. A majority of cell culturing has been carried out by ... The adsorption of small biological molecules from cell culturing samples as well as the release of oligomers into the culture ... where the device is open for the introduction and culturing of cells, and can be sealed prior to analysis. Cells and proteins ...
Air liquid interface cell culture (ALI) is a method of cell culture by which basal stem cells are grown with their basal ... This method of cell culture aims to be used to study fundamental aspects of the respiratory epithelium, such as cell-to-cell ... The cell culture method used involves the isolation of cells into culturing with growth factors to grow over time. Once the ... Air-liquid interface cell culture compares to standard cell culture practices by specifically aiming to restore the ...
3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high- ... Because of the ability of 3D cell culturing by magnetic levitation to bring cells together, co-culturing different cell types ... Standard monolayer cell culturing on tissue culture plastic has notably improved our understanding of basic cell biology, but ... Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio- ...
The European Collection of Authenticated Cell Cultures houses and supplies cell lines. It is part of the Culture Collections of ... ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 species ... the culture collections website alongside a range of services such as Mycoplasma testing and training courses for cell culture ... Culture Collections Official home page v t e (Use dmy dates from April 2022, Articles with topics of unclear notability from ...
Different cell lines and cell types have been cultured in NFC, including e.g. differentiation of human hepatic cells to ... like cell culturing. GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. NFC fiber network structure ... functional organotypic cultures, and proliferation of human pluripotent stem cells. Organotypic liver cell cultures can be used ... Additionally, 3D cell culture using wood-based nanocellulose hydrogel can be used for tissue engineering. Ahmed, Enas M. (2015- ...
Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing ... While SILAC had been mostly used in studying eukaryotic cells and cell cultures, it had been recently employed in bacteria and ... Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects ... Amanchy R, Kalume DE, Pandey A (January 2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying ...
Dawn news election cells. Dawn news election cells. Retrieved 21 February 2015.- Hoodbhoy, Pervez. "Noam Chomsky interviewed by ... Pakistani culture, Pakistani social culture, Himalayan culture, South Asian culture). ... The Culture of Pakistan (Urdu: پاکستانی ثقافت Pākistāni S̱aqāfat) is based in the Indo-Persian cultural matrix that constitutes ... The consumption of tea in Pakistan, called chai (چائے), in Urdu, is of central significance to Pakistani culture. It is one of ...
doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. Although the majority of Afanasievo burials ... Chalcolithic cultures of Asia, Bronze Age cultures of Asia, Archaeological cultures of China, Archaeological cultures in ... In particular, contacts between the Afanasievo culture and the Majiayao culture and the Qijia culture are considered for the ... The Afanasievo culture, or Afanasevo culture (Afanasevan culture) (Russian: Афанасьевская культура Afanas'yevskaya kul'tura), ...
Cell. 183 (4): 890-904. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. (Articles with short ... Nearby Saka cultures were the Tagar Culture of the Minusinsk Basin, as well as the Pazyryk Culture (ca. 500-200 BCE) in the ... 93.631836 The Sagly-Bazhy culture or Sagly/Uyuk culture, also known as Chandman culture in Mongolia (Ulaangom cemetery), refers ... It is the last stage of the Uyuk culture. This period of Scythian culture covers a period from the 5th century BCE to the 2nd ...
Cell. 183 (4): 890-904. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. S2CID 214725595. Zhang, Fan ... Dental analysis has shown that the Chemurchek culture consumed ruminant dairy products. The people of the Chemurchek culture ... The Chemurchek culture had various characteristics of West European origin. Chemurchek funerary and ritual structures ... The mortuary position of the deceased (supine position with flexed legs) is similar to that of the Afanasievo culture. ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Lee 2023, ""SlabGrave" was ... Archaeological cultures of East Asia, Archaeological cultures of Northern Asia, Bronze Age cultures of Asia, Iron Age cultures ... The Ulaanzuukh culture (c. 1450-1150 BCE) is an archaeological culture of the Late Bronze Age eastern Mongolia. It likely ... Indeed, the genetic profile of individuals belonging to this culture is genetically identical to the Slab Grave culture ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Antonio Sagona. Rethinking the Kura- ... mun INDUS VALLEY CIVILIZATION Corded Ware Culture Yamnaya Culture Kura- Araxes Afanasievo culture Botai culture Sarazm culture ... The Kura-Araxes culture (also named Kur-Araz culture, Mtkvari-Araxes culture, Early Transcaucasian culture) was an ... Kura-Araxes culture, Bronze Age cultures of Asia, Chalcolithic cultures of Asia, Archaeological cultures of West Asia, ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. Meakin, Annette M. B ... Other post-Xiongnu cultures, such as the Kokel Culture have also been recorded nearby. Tashtyk culture mask Tashtyk culture ... The Tashtyk culture was Late Iron Age archaeological culture that flourished in the Yenisei valley in Siberia from the 1st ... it was preceded by the Tagar culture. The Tashtyk culture was first surveyed by the Russian archaeologist Sergei Teploukhov. ...
Marchi, Nina (2022). "The genomic origins of the world's first farmers". Cell. 185 (11): 1842-1859. doi:10.1016/j.cell.2022.04. ... Archaeological cultures of Europe, Neolithic cultures of Europe, Archaeological cultures in Austria, Archaeological cultures in ... Archaeological cultures in France, Archaeological cultures in Germany, Archaeological cultures in Hungary, Archaeological ... cultures in Moldova, Archaeological cultures in the Netherlands, Archaeological cultures in Poland, Archaeological cultures in ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. Damgaard, P. B.; et ... In 2009, a genetic study of ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and the ... The Tagar culture was a Bronze Age archeological culture which flourished between the 8th and 1st centuries BC in South Siberia ... The Tagar culture was preceded by the Karasuk culture. They are usually considered a mixed population descended from peoples of ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Török, Tibor (July 2023). " ... Karasuk culture (Ural to Altai) Aldy-Bel culture Okunevo culture Pazyryk culture The origins of the Karasuk culture are complex ... 2009) published a genetic study of ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and ... Bronze Age cultures of Asia, Archaeological cultures of Siberia, Iranian archaeological cultures, Archaeological cultures in ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. Rogers, Leland Liu; ... various Saka cultures such as the Tagar culture, the Pazyryk culture and the Aldy-Bel culture for a period of several centuries ... Archaeological cultures of East Asia, Archaeological cultures of Northern Asia, Bronze Age cultures of Asia, Iron Age cultures ... The Slab-Grave culture is an archaeological culture of Late Bronze Age and Early Iron Age Mongolia. The Slab-Grave culture ...
Cell. 184 (18): 4612-4625.e14. doi:10.1016/j.cell.2021.07.013. ISSN 0092-8674. PMC 8445022. PMID 34352227. Broushaki, F; Thomas ... Epipalaeolithic cultures, Archaeological cultures in Israel, Archaeological cultures in Jordan, Archaeological cultures in ... Natufian culture, Industries (archaeology), Archaeological cultures of West Asia, Archaeological cultures of the Near East, ... The Natufian culture (/nəˈtuːfiən/) is a Late Epipaleolithic archaeological culture of the Neolithic prehistoric Levant in ...
Cell. 163 (3): 571-582. doi:10.1016/j.cell.2015.10.009. PMC 4644222. PMID 26496604., S14-15. This sample is marked "RISE505". ... MIDDLE KINGDOM Kerma culture Longshan Culture Qijia culture Baodun culture Shijiahe culture Abashevo Culture Catacomb Culture ... Andronovo culture Sintashta culture BMAC Vakhsh Tarim mummies Okunev culture Karakol culture Samus culture Ulaanzuukh culture ... 2009) published a study of the ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and the ...
Sagly culture Jeong, Choongwon (12 November 2020). "A Dynamic 6,000-Year Genetic History of Eurasia's Eastern Steppe". Cell. ... The Chandman culture, also Chandmani culture, was a nomadic culture that existed in northwestern Mongolia and southern Siberia ... The Chandman culture has been linked to the nearby cultures at Sagly and Uyuk. The Chandman population seems to have been in ... It is associated with the Scythian/Saka horizon, and is part of the more general Saka Uyuk culture. The Chandman culture was ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Török, Tibor (July 2023). " ... MIDDLE KINGDOM Kerma culture Longshan Culture Qijia culture Baodun culture Shijiahe culture Abashevo Culture Catacomb Culture ... Andronovo culture Sintashta culture BMAC Vakhsh Tarim mummies Okunev culture Karakol culture Samus culture Ulaanzuukh culture ... Sintashta material culture also shows the influence of the late Abashevo culture, derived from the Fatyanovo-Balanovo culture, ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. "Okunev Culture". The Great Soviet ... Okunev culture shares some elements of its material culture, including pottery. with a number of local contemporaneous cultures ... Okunev culture'), sometimes also Okunevo culture, was a south Siberian archaeological culture of pastoralists of the early ... The Okunev people of Tuva show stronger influence from the Pits culture and early Catacomb culture of Ukraine. He argues that ...
Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Cell Culture ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... A 3D cell culture model showed cell growth similar to that of in vivo than did a monolayer culture, and all three cultures were ...
... including submerged cell culture, co-culture, and modeling of air–liquid and liquid–liquid interfaces. Not only is ... System consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over cells ... As a result, cultures are exposed to more physiologically relevant conditions, increasing the predictive value of in vitro ... The Quasi Vivo System is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of ...
Single-Cell RNA Sequencing of Blood and Ileal T Cells From Patients With Crohns Disease Reveals Tissue-Specific ... Klarman Cell Observatory The Klarman Cell Observatory is systematically defining mammalian cellular circuits, how they work ... Cell Stem Cell. 2018;23(4):557-571.e8. doi:10.1016/j.stem.2018.09.008. ... Identification of RIOK2 as a master regulator of human blood cell development. Nat Immunol. 2022;23(1):109-121. doi:10.1038/ ...
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Synthesized cell culture process sets stage for more efficient cancer research Peer-Reviewed Publication Tokyo University of ... Synthesized cell culture process sets stage for more efficient cancer research. Tokyo University of Agriculture and Technology ... To study this new cell culture approach, the researchers focused on bladder cancer in dogs, a disease which occurs at a rate ... image: CK5 expression, a basal cell marker, in the 2.5D organoid cells (right) and parental 3D organoids (left). view more ...
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Human Food Made with Cultured Animal Cells. The ability to take a small number of cells from living animals and grow them in a ... Advancements in cell culture technology enable scientists to use animal cells obtained from livestock, poultry, or seafood to ... USDA and FDA Announce a Formal Agreement to Regulate Cell-Cultured Food Products from Cell Lines of Livestock and Poultry. ( ... Statement from USDA Secretary Perdue and FDA Commissioner Gottlieb on the regulation of cell-cultured food products from cell ...
Because it is made with animal cells, many processes for developing and scaling a lab-cultured meat product will mirror those ... What is cell-cultured meat?. Cell cultured meat, also known as cultivated, cultured, or lab-grown meat, is a product of ... Because cell-cultured meat is made with animal cells, many processes for developing and scaling a cell-cultured meat product ... Scale cultured meat production with monoclonal cell lines. Animal cells are usually engineered to produce different biologics, ...
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The market also involves ongoing innovations to improve cell culture conditions and address the evolving requirements of cell- ... and maintenance of cells outside their natural environment. In the context of a market, the cell culture media market ... Cell culture media refers to a complex mixture of nutrients, growth factors, salts, vitamins, and other components essential ... Access a sample of the report and more information about: Cell Culture Media Market Report. About Global Market Studies USA: ...
... an effective method for the isolation of human SC cells and provides a cellular model to study the potential role of SC cells ... Isolation, culture, and characterization of endothelial cells from Schlemms canal Invest Ophthalmol Vis Sci. 1998 Sep;39(10): ... To date, TM cells are routinely cultured and used as a model by several laboratories. However, there has been only limited ... The current objective was to develop a technique for selective isolation and culture of endothelial cells from human SC. ...
USDA and FDA announce a formal agreement to regulate cell-cultured food products from cell lines of livestock and poultry. ... cell-cultured meat. ARTICLES. USDA seeks comments on the labeling of meat and poultry products derived from animal cells. ... Home » Keywords: » cell-cultured meat. Items Tagged with ... Cargill invests in cultured meat company Aleph Farms. Industry ... In July 2019, several states "declared war" on advertising plant- or cell-based products as "meat.". ...
... Originally Published MDDI ... Biomedical engineers at Johns Hopkins performed a study on heart cell cultures and found that the cause of these dangerous ...
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3-D cell culture. What Are Organoids and How Are They Made? ... Using 3-D Cell Culture Systems for Extracellular Vesicle ... Mini-Guts to the Rescue: Introducing 3-D Organoid Cell Cultures. The Scientists Creative Services Team and MilliporeSigma , ... 3-D cell culture recapitulates in vivo environments for generating physiologically relevant extracellular vesicles. ... Scientists fiddle with formulas to boost the growth of their stem cell cultures. ...
The market also involves ongoing innovations to improve cell culture conditions and address the evolving requirements of cell- ... and maintenance of cells outside their natural environment. In the context of a market, the cell culture media market ... Cell culture media refers to a complex mixture of nutrients, growth factors, salts, vitamins, and other components essential ... Access a sample of the report and more information about: Cell Culture Media Market Report. About Global Market Studies USA: ...
COsub2sub Incubator simplifies benchtop cell culturing. - Mar 31, 2010 - Thermo Fisher Scientific ... The easily stackable Thermo Scientific Midi 40 CO2 incubator is ideal for cell culturing scientists who need smaller, discrete ... CO2 Incubator simplifies benchtop cell culturing.. Thermo Fisher Scientific Mar 31, 2010 ... Thermo Scientific Midi 40 CO2 Incubators enable simple, reliable benchtop culturing. MUNICH - Thermo Fisher Scientific Inc., ...
Kelm and others published 3D cell culture for compound de-risking , Find, read and cite all the research you need on ... made of various ratios of PU and PLLA showed similar cell viability to that of cells cultured on a tissue cell culture system ( ... High-Throughput Cancer Cell Sphere Formation for 3D Cell Culture. June 2017 · Methods in molecular biology (Clifton, N.J.) ... Here, we culture primary mouse motor neurons and immortalised CAD cells, a cell line derived from the central nervous system, ...
Monitoring and maintaining robust cell health and protein quality are critical to scale from the lab to commercialization. ... Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK ... The Vi-CELL MetaFLEX is designed to be used with cell cultures of multiple cell lines - important for potentially reducing ... Cell density and viability are critical to the cell culture process and for determining culture physiology in response to ...
In this article, we discuss key considerations before using a shaker in a CO2 incubator for the cultivation of mammalian cells ... How to quantify bacterial cultures - From CFU and OD to counting chamber. ...
For cell-cultured meat to go mainstream, the cost of the media used to grow animal cells must come down. A lot. And that will ... Cultivated meat, cell-cultured meat, cell-based meat? USDA seeks to nail down nomenclature as products get closer to market in ... Cell-cultured may be the best way to label seafood grown from animal cells in bioreactors, according to key stakeholders in ... Dutch cell-cultured meat startup Meatable - which is working with porcine and bovine induced pluripotent stem cells [iPSCs] - ...
What to know when discovering the various types of cells used in cell culture for biomedical research. Learn how to grow and ... Feeder cells. Finally, lets talk about feeder cells. These cells support the growth and maintenance of other cells in culture. ... For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and ... Examples include blood cells and some cancer cells. Culturing suspension cells can be tricky because they need specialized ...
... to counteract undesired effects of chemotherapy. ... Polysaccharides isolated from Echinacea purpurea herba cell cultures to counteract undesired effects of chemotherapy. - ... Polysaccharides isolated from Echinacea purpurea herba cell cultures to counteract undesired effects of chemotherapy--a pilot ... injections of 2 mg of a polysaccharide fraction isolated from Echinacea purpurea herb cell cultures (EPS-EPO VIIa). The median ...
For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concent … ... Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells Acta Diabetol. 1996 Jul;33 ... For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; there- ... These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells. ...
  • Cell culture media filtration prevents bioreactor contamination. (sigmaaldrich.com)
  • Cell cultured meat, also known as cultivated, cultured, or lab-grown meat, is a product of cellular agriculture , that uses tissue samples from living animals to grow sustainable, humane meat in a bioreactor. (moleculardevices.com)
  • Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
  • While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
  • Bioreactor flasks have a large surface area and volume, allowing for increased cell density and greater antibody production. (coleparmer.com)
  • Learn about cell culture media, large volume cell banking, and single-use bioreactor versus stainless steel bioreactor design in cell culture development. (ncsu.edu)
  • Cell-cultured meat pioneer UPSIDE Foods (formerly Memphis Meats) has brought in high-flying PepsiCo exec Amy Chen as its chief operating officer as it prepares to launch its first consumer product - UPSIDE chicken - later this year, pending regulatory. (foodnavigator-usa.com)
  • Advancements in cell culture technology enable scientists to use animal cells obtained from livestock, poultry, or seafood to produce food products. (usda.gov)
  • On March 7, 2019, FDA and FSIS agreed to establish a joint regulatory framework for human foods made from cultured cells of livestock and poultry to help ensure that any such products brought to market are safe, unadulterated, and truthfully labeled. (usda.gov)
  • The USDA has issued an advance notice of proposed rulemaking (ANPR) to solicit comments on how to label cell-cultured meat and poultry as startups in the space edge closer to commercialization, but says it will review labels submitted before the rulemaking. (foodnavigator-usa.com)
  • Mr. Sylvester says, "USDA has not yet invited public comment on the labelling of cell-cultured meat & poultry but is expected to do so in due course. (cov.com)
  • The current objective was to develop a technique for selective isolation and culture of endothelial cells from human SC. (nih.gov)
  • Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)
  • We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
  • endothelial cells. (lu.se)
  • LAS VEGAS, NEVADA, UNITED STATES, November 21, 2023 / EINPresswire.com / -- The Cell Culture Media Market is driven by increasing demand for biopharmaceuticals, rising investments in life sciences research, and advancements in cell-based therapies. (wgntv.com)
  • Discover how to thaw cells and maintain viability. (atcc.org)
  • Producing highly productive cell lines and optimized cell culture conditions requires an understanding of the contributions of many key variables: cell culture media, cell growth rates, cell viability, protein expression levels, and other quality attributes including size, aggregation status, glycosylation and stability. (beckman.com)
  • Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)
  • Such improvements include ensuring greater uniformity of the device, reducing end-user assembly procedures, and enhancing viability for the long-term culturing period that is often needed for neurons. (sbir.gov)
  • Our Mononuclear Cell Medium ensures ideal cell viability under cell culture conditions up to 48 hours. (promocell.com)
  • The effect of the materials on the cultivated fibroblasts was analyzed in terms of short-term and long-term cell growth as well as in viability and cell proliferation assays. (bvsalud.org)
  • Mycoplasmas frequently contaminate cell cultures. (atcc.org)
  • Under this agreement, FDA will oversee the collection, growth, and the differentiation of living cells into various cell types, such as proteins and fats. (usda.gov)
  • Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis. (thermofisher.com)
  • Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. (thermofisher.com)
  • Materials and methods: Two-dimensional (2D) cell cultures, in comparison with original tissues, does not fully reproduce in vivo cell growth and differentiation. (srce.hr)
  • Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces. (bvsalud.org)
  • This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast -like (osteo-1) cells cultured on chemically modified titanium surfaces. (bvsalud.org)
  • Cell differentiation was influenced by rhBMP-2, as demonstrated by a significant increase in collagen content after 7 days of culture (P (bvsalud.org)
  • Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface. (bvsalud.org)
  • Traditional random cultures allow limited access to subcellular compartments (axons, dendrites, synapses) due to extensive and haphazard growth of neurons. (sbir.gov)
  • Our long-term goal is to provide robust, user-friendly, and cost effective culture platforms that can optically, fluidically, and biochemically access neurons and their subcellular compartments. (sbir.gov)
  • The significance of the proposed work is to improve research scientists' ability to visualize, manipulate, and measure cultured neurons leading to greater understanding of the underlying causes of neurological diseases. (sbir.gov)
  • Double-staining studies with specific cell markers to distinguish astrocytes, neurons and oligodendrocytes were performed. (lu.se)
  • COMT immunoreactivity colocalized with a specific oligodendrocyte marker galactocerebroside in cells displaying oligodendrocyte morphology, flat cells displaying type-1 astrocyte morphology and glial fibrillary acidic protein, in branched cells displaying type-2 astrocyte morphology and in cell bodies of neurons, the processes of which displayed neurofilament immunoreactivity. (lu.se)
  • The results suggest that COMT is synthesized by cultured astrocytes, oligodendrocytes and neurons. (lu.se)
  • In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
  • As a result, cultures are exposed to more physiologically relevant conditions, increasing the predictive value of in vitro experiments. (genengnews.com)
  • Organoids are in vitro models that closely replicate the complex physiology of native tissues while also being accessible, reproducible, and scalable like traditional culture methods. (sigmaaldrich.com)
  • Cell culture media refers to a complex mixture of nutrients, growth factors, salts, vitamins, and other components essential for the in vitro growth, proliferation, and maintenance of cells outside their natural environment. (wgntv.com)
  • A major challenge with this technique lies in achieving sufficient T cell growth and survival in vitro to obtain a sufficient dose of reactive T cells. (medgadget.com)
  • Collagen I is used in both 2D and 3D cell culture to make the in vitro cellular environment more in vivo -like. (thermofisher.com)
  • A well-defined in vitro three-dimensional culture of human endometrium and its applicability to endometrial cancer invasion. (thermofisher.com)
  • Therefore, significant efforts have been made toward the development of more realistic three-dimensional (3D) in vitro cell culture models that would better mimic tissue physiology. (srce.hr)
  • Background: The success of in vitro-fertilization (IVF) cycles is determined in large part by the quality of embryo cleavage, which in turn, is dependent on the quality of the embryo culture media (CM). Many factors can influence the quality of embryo CM, one of which is the levels of Cell Free Deoxyribonucleic acid (DNA). (who.int)
  • Six parasites ont montré une résistance in vitro au stibogluconate de sodium en utilisant le test de détection des amastigotes dans les macrophages J774 murins. (who.int)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)
  • Starter cultures and growth media may be purchased from the Collection for academic or commercial research with no IP or licensing restrictions. (umass.edu)
  • Our recombinant cell culture supplements include albumin, transferrin, insulin, trypsin, NAO growth factors and transport proteins for production of mAbs and recombinant proteins, as well as cell and gene therapies. (sigmaaldrich.com)
  • This essential laboratory process covers the thaw, growth, propagation, and cryopreservation of cells that have been isolated from a donor organism. (atcc.org)
  • Additional growth factors and hormones are added to promote growth or attachment to the culture vessel, often via undefined adjuncts such as serum. (atcc.org)
  • This results in a substantial increase in cell growth surface area compared to traditional vessels of comparable cubic footprint. (corning.com)
  • Hear from our experts on their experiences and insights in primary cell culture, single cell culture, cell growth, and more. (corning.com)
  • We offer a broad portfolio of solutions for 3D cell culture including products for supporting spheroid growth, scaffolds, hydrogels, organoids, ULA plates, and other cultureware. (sigmaaldrich.com)
  • There is a long history of scientific advances in biology, biochemistry, and engineering that have led to the innovations enabling the growth of animal cells outside of the animal itself, in a controlled environment, for food. (usda.gov)
  • To make food, a small number of cells are taken from the cell bank and placed in a tightly controlled and monitored environment (e.g., a very large, sealed vessel) that supports growth and cellular multiplication by supplying appropriate nutrients and other factors. (usda.gov)
  • After the cells have multiplied many times over into billions or trillions of cells, additional factors (e.g., protein growth factors, new surfaces for cell attachment, additional nutrients) are added to the controlled environment to enable the cells to differentiate into various cell types and assume characteristics of muscle, fat, or connective tissue cells. (usda.gov)
  • Animal cells are usually engineered to produce different biologics, including growth factors, hormones, and antibodies that are commonly used as therapies today. (moleculardevices.com)
  • The Cell Culture Media Market study includes comprehensive analysis of biotechnological market trends, growth factors, and challenges. (wgntv.com)
  • Scientists fiddle with formulas to boost the growth of their stem cell cultures. (the-scientist.com)
  • Device's rapid recovery rates promote optimal cell growth while direct heating provides temperature control with uniformity of 0.1°C. (thomasnet.com)
  • Rapid recovery rates promote optimal cell growth and highly efficient direct heating design provides extremely accurate temperature control with a uniformity of 0.1°C. (thomasnet.com)
  • Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
  • These cells support the growth and maintenance of other cells in culture. (coleparmer.com)
  • Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
  • For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
  • And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)
  • They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)
  • After conducting thorough research on the historical and current growth parameters of the Cell Culture Market, the growth prospects of the market are obtained with maximum precision. (marketresearch.com)
  • The macroeconomic factors affecting the growth of the Cell Culture Market are provided in this section and the impact of these macroeconomic indicators on the Cell Culture Market is analyzed. (marketresearch.com)
  • The drivers and restraints impacting the growth of the Cell Culture Market are explained in this chapter. (marketresearch.com)
  • Researchers at the Harvard Wyss Institute have developed a biomaterial scaffold that mimics the actions of antigen-presenting cells (APCs) in stimulating T cell growth and survival. (medgadget.com)
  • The researchers designed a biomaterial scaffold that provides pro-survival and pro-growth biological cues to T cells, just like an APC in the body. (medgadget.com)
  • Perform cell culture expansion and experience 500cm 2 total growth area within the footprint of a standard flask using Thermo Scientific™ Nunc™ TripleFlask™ Treated Cell Culture Flasks. (thomassci.com)
  • Three layers of growth surfaces provide a total culture area of 500 cm 2 in the same footprint as a standard Nunc 175cm 2 flask. (thomassci.com)
  • A thin coating of diluted collagen solution supports cell attachment and growth of some primary cells, including hepatocytes. (thermofisher.com)
  • Pro-adhesive and chemotactic activities of thrombospondin-1 for breast carcinoma cells are mediated by alpha3beta1 integrin and regulated by insulin-like growth factor-1 and CD98. (thermofisher.com)
  • Basic concepts and advantages of 3D cell cultures, as well as different approaches in technologies that enable the cell growth in 3D will be presented here. (srce.hr)
  • Possible applications of 3D cell culture in drug discovery will be discussed, and example of formation of spherical growth of three different human breast cancer cells (MDA-MB-231, SK-BR-3 and T-47D cells) in 3D format will be shown. (srce.hr)
  • The firm said that the silicone surfaces adhere to a wide range of extracellular matrices for optimal cell growth and conditions. (scienceboard.net)
  • Gain a fundamental understanding of growth of mammalian and insect cells used for the production of recombinant proteins, antibodies, gene therapy vectors, and exosomes. (ncsu.edu)
  • The groups having received the materials following the setting reaction had a lower number and percentage of viable cells in comparison to the control, but the materials allowed cell growth throughout the experiment. (bvsalud.org)
  • Conclusion: All glass ionomer cements tested were toxic prior to the setting reaction.However after setting reaction, allowed cell growth. (bvsalud.org)
  • These best practices form the basis of several cell culture guides , webinars , and video tutorials that detail the initiation, expansion, and cryopreservation of continuous cell lines, primary cells, hTERT-immortalized primary cells, and organoids. (atcc.org)
  • image: CK5 expression, a basal cell marker, in the 2.5D organoid cells (right) and parental 3D organoids (left). (eurekalert.org)
  • Professor Usui's team set out to create a type of organoids without the use of cell-stimulating supplements and Matrigel, a costly product derived from stem cells harvested from genetically engineered rodents. (eurekalert.org)
  • The team created the organoids by culturing the captured diseased cells in a protein-rich medium. (eurekalert.org)
  • Once the team succeeded in creating organoids from the dogs' diseased cells, they tested their response to three common anti-cancer drugs as well as several antibodies. (eurekalert.org)
  • The cells became tumors as anticipated, giving the team further evidence that 2.5D organoids may be an accurate and effective research medium. (eurekalert.org)
  • Choose colon organoids, lung organoid culture systems, and patient-derived organoids from our biobank. (sigmaaldrich.com)
  • Once founded, the cell line can be propagated into several flasks and frozen. (atcc.org)
  • April 20, 2021 -- Advanced BioMatrix has introduced its new line of CytoSoft flasks for cell culture. (scienceboard.net)
  • The CytoSoft flasks have more surface area than the company's CytoSoft 6, 24, and 96-well plates, enabling greater cell expansion and passaging on an in vivo-like substrate, Advanced BioMatrix said. (scienceboard.net)
  • We are deeply committed to providing you with robust products to meet your mammalian cell culture needs. (sigmaaldrich.com)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
  • Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
  • Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
  • This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)
  • Promotes monolayer adherent cell culture. (thomassci.com)
  • Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (cdc.gov)
  • In Hep-2C cells, the cellular monolayer was partially destroyed. (cdc.gov)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • Regulatory oversight of human foods comprised of or containing cultured animal cells depends on the animal species used as the original source of cultured cells, and it is based on the agencies' existing jurisdiction over products. (usda.gov)
  • Because cell-cultured meat is made with animal cells, many processes for developing and scaling a cell-cultured meat product will mirror those in biologics production, specifically cell line development . (moleculardevices.com)
  • Our highly validated, optimized products, including upstream chemicals, supplements, chemically defined cell culture media, bioreactors and clarification filters, coupled with decades of expertise, provide convenient solutions to even the most complex challenges. (sigmaaldrich.com)
  • A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale. (sigmaaldrich.com)
  • It includes a wide range of products such as classical media, serum-free media, chemically defined media, and specialty media designed to support the specific needs of diverse cell types and applications, such as drug development, vaccine production, and regenerative medicine. (wgntv.com)
  • Aim: The aim of the present study was to assess the toxicity of different chemically activated GICs in fibroblast cell cultures post-agglutination (PA) and post-setting reaction (PS). (bvsalud.org)
  • Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid -etched) titanium surfaces in 3 different types of medium control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. (bvsalud.org)
  • Schlemm's canal cell isolates reacted with antibodies specific for CD44 (hyaluron receptor), CD54 (intercellular adhesion molecule-1, ICAM-1), tissue-type plasminogen activator, and TM-inducible glucocorticoid-responsive protein-myocilin (TIGR-MYOC). (nih.gov)
  • Unlike TM cells, however, TIGR-MYOC protein was not induced in SC cells after long-term dexamethasone treatment. (nih.gov)
  • Cell culture biotech startup Avant has launched a multifunctional protein for the active beauty market made from cultivated fish cells - an ingredient it says will carve out an entirely new segment in anti-ageing and protective skin care. (cosmeticsdesign-europe.com)
  • Monitoring and maintaining robust cell health and protein quality are critical to scale from the lab to commercialization. (beckman.com)
  • They loaded the rods with Interleukin-2, a protein produced by APCs that can enhance T cell survival, and coated them using a lipid layer, to mimic the APC cell membrane. (medgadget.com)
  • Collagen I initiates endothelial cell morphogenesis by inducing actin polymerization through suppression of cyclic AMP and protein kinase A." Journal of Biological Chemistry 278, no. 1 (January 2003): 327-334. (thermofisher.com)
  • Nevertheless, mycoplasmas can have disastrous effects on eukaryotic cells, as they can alter every cellular function (proliferation, protein synthesis, susceptibility to viral infection, etc. (invivogen.com)
  • Scientists also added various protein extracts from animals to make the cells grow. (lu.se)
  • Examining the urine under a microscope may be done to check for the presence of red and white blood cells, crystals, and casts (impressions of the kidney tubules created when urinary cells, protein, or both precipitate out in the tubules and are passed in the urine). (msdmanuals.com)
  • The cells are generally grown in plastic or glass vessels. (atcc.org)
  • To prevent this, it's important to create single-cell suspensions by gently pipetting or using specialized culture vessels. (coleparmer.com)
  • For suspension cells, use conical tubes or specialized culture vessels to keep cells in suspension and prevent aggregation. (coleparmer.com)
  • Best practices for reviving cells from cryopreservation are critical to ensuring unambiguous experimental results in basic research and industrial processes. (atcc.org)
  • FSIS and FDA have held public meetings to better understand the science of animal cell culture technology, potential hazards, labeling considerations, and to listen to consumer concerns. (usda.gov)
  • Regulatory jurisdiction transitions from FDA to FSIS during the harvesting stage of the cell-culturing process. (usda.gov)
  • Because of the invaluable nature and numerous applications of cell lines in life science research, it is critical that they are handled carefully to not only avoid contamination but to protect human safety. (atcc.org)
  • This ebook provides expert guidance on cell culture techniques as well as best practices and was designed to serve as a convenient guide to cell culture basics as well as more advanced topics like troubleshooting problems and avoiding contamination. (corning.com)
  • Say goodbye to cell culture contamination nightmares and hello to happy cells. (coleparmer.com)
  • Mycoplasma contamination of cells often goes unperceived. (invivogen.com)
  • Contamination happens quickly and spreads fast, so labs that rely on cell cultures must test them regularly. (invivogen.com)
  • This universal sensitivity, combined with an easy-to-use format, makes PlasmoTest™ a reliable, simple and cost-effective product for routine testing of all cell cultures at risk of contamination. (invivogen.com)
  • Cell culture is a fundamental tool used in areas as wide-ranging as drug development, toxicity testing, disease modeling, and regenerative medicine. (atcc.org)
  • Corning offers a line of products designed for large-scale of stem cell-derived extracellular vesicles (EVs) to impact regenerative medicine and the treatment of inflammatory conditions. (corning.com)
  • The human embryo is a source of vital cells used in regenerative medicine, as well as a powerful symbol of life. (lu.se)
  • The third part of the bioprocess webinar series, this webinar presents key advancements in immune cell therapy research. (corning.com)
  • The processing overview and technological advancements in the Cell Culture Market are also provided. (marketresearch.com)
  • We used colon carcinoma multicellular spheroids containing hypoxic and quiescent cells in core areas for this purpose. (researchgate.net)
  • Invasion of interstitial matrix by a novel cell line from primary peritoneal carcinosarcoma, and by established ovarian carcinoma cell lines: role of cell-matrix adhesion molecules, proteinases, and E-cadherin expression. (thermofisher.com)
  • Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an. (cdc.gov)
  • Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)
  • Whether you are producing antibodies, recombinant proteins, vaccines or viral vectors for gene therapies, our cell line platforms can accelerate your upstream progress. (sigmaaldrich.com)
  • Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK 293 or CHO cells, an expression or infection system and selection, followed by process and media optimization in small scale systems such as 96-well plates or bench-scale bioreactors. (beckman.com)
  • RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (cdc.gov)
  • Neisseria gonorrhoeae culture is indicated in the diagnosis of N gonorrhoeae infection. (medscape.com)
  • Positive culture indicates active infection with N gonorrhoeae . (medscape.com)
  • Moreover, symptoms showed an association with culture recovery when specimens were collected at urethral and rectal infection sites. (medscape.com)
  • Endocervical culture is a laboratory test that helps identify infection in the female genital tract. (medlineplus.gov)
  • John Pattison, the firm's CEO who has previously worked for US cell-based meat company New Age Meats, founded the business with chief scientific officer Ian Johnson having come up with the idea to create sustainable seafood products with a "dramatically lower" environmental footprint in 2019. (just-food.com)
  • Using its own technology, Cultured Decadence is developing seafood 'meat' using the cells of lobster in the first instance, with potential expansion into other shellfish such as crab, shrimp and scallops. (just-food.com)
  • Brian Sylvester spoke with Food Navigator about the labeling of cell-cultured seafood. (cov.com)
  • As for when we can expect a final decision on cultured seafood labelling, he says, "It's too early to anticipate a precise timeline for FDA's decision-making…The first step will be for FDA to gather and analyze the requested data and information, while concurrently aligning on key principles with USDA's Food Safety and Inspection Service. (cov.com)
  • Using human pluripotent stem cells, scientists engineered an amniotic sac-like structure, potentially useful for uncovering the elusive cellular signals that lead to infertility. (the-scientist.com)
  • To meet volume demands, biopharmaceutical companies have built large-scale facilities with multiple 10,000 L (or even larger) cell culture bioreactors. (beckman.com)
  • This research is innovative because we seek to shift current research paradigms through the development of novel cell-based tools to isolate synapses that maintain intact cell morphology in the absence of somata or glia. (sbir.gov)
  • Some cells remained isolated but with altered morphology ( Figure 1F ). (cdc.gov)
  • This chapter includes a detailed analysis of the historical Cell Culture Market (2014-2021), along with an opportunity analysis for the forecast period (2022-2032). (marketresearch.com)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
  • He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Cells embedded in 3D hydrogels can polarize, differentiate and have functional attributes that more closely resemble physiological tissues. (sigmaaldrich.com)
  • The entire process of going from animal tissues to cultured meat products described above is time-consuming, labor-intensive, and prone to error if done in a manual fashion. (moleculardevices.com)
  • Collagen I is the most common fibrillar collagen for cell culture and is found in skin, bone, tendons, and other connective tissues. (thermofisher.com)
  • The Quasi Vivo ® System consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over cells. (genengnews.com)
  • The Quasi Vivo System is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, co-culture, and modeling of air-liquid and liquid-liquid interfaces. (genengnews.com)
  • 3-D cell culture recapitulates in vivo environments for generating physiologically relevant extracellular vesicles. (the-scientist.com)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Our off-the-shelf and customizable bioprocessing cell culture media (CCM) products enhance productivity in upstream mAb, vaccine, gene/cell therapy processes. (sigmaaldrich.com)
  • The Corning HYPERFlask cell culture vessel helps deliver scalable vaccine production and development. (corning.com)
  • Title : Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived JE vaccine (JE-VC) Personal Author(s) : Walter, Emmanuel B.;Hills, Susan L. (cdc.gov)
  • Discover best practices how to subculture adherent and suspension cultures. (atcc.org)
  • One big challenge with suspension cells is aggregation, which means your cells clump together and make it difficult to maintain single-cell suspensions. (coleparmer.com)
  • This ethnological compilation thesis follows a national branch of a foetal cell transplantation trial through successes as well as challenges in processing foetal material into an effective, transplantable cell suspension. (lu.se)
  • The cell suspension is conceptualized as a bio-object, and explored as something that produces new knowledge, emotions and logistical and ethical negotiations. (lu.se)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • Cells grown on structural scaffolds engineered to mimic the ECM can be powerful, predictive models of physiological processes. (sigmaaldrich.com)
  • Some cells from the sample are selected, screened, and grown to make a "bank" of cells to store for later use. (usda.gov)
  • Conclusions: Although biological significance of obtained data from 2D and 3D cell cultures is still poorly understood, discrepancy of compunds activity illustrated importance of implementation 3D cell culture assays in early part of drug discovery process. (srce.hr)
  • The synthesized tumor cells allow scientists to diagnose cancer and optimize treatment without putting the patient through tiresome rigors of chemotherapy trial and error. (eurekalert.org)
  • Scientists typically start with a sample of cells from the tissue of an animal or fish, a process that typically does not permanently harm or kill the animal. (usda.gov)
  • The easily stackable Thermo Scientific Midi 40 CO2 incubator is ideal for cell culturing scientists who need smaller, discrete workspaces or who require smaller sample throughput. (thomasnet.com)
  • These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. (wikipedia.org)
  • Most freshly isolated, primary cell cultures undergo senescence, the process by which cells stop dividing after undergoing a certain number of cell divisions. (atcc.org)
  • Of the 20 pairs of eyes that were cannulated, primary cells were obtained from 13. (nih.gov)
  • What are the challenges of culturing primary cells compared to established cell lines? (coleparmer.com)
  • Culturing primary cells presents unique challenges compared to established cell lines. (coleparmer.com)
  • Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
  • Our approach closely mimics how APCs present their stimulating cues to primary T cells on their outer membrane and how they release soluble factors that enhance the survival of the T cells," said David Mooney, a researcher who led the study. (medgadget.com)
  • Primary human mononuclear cells (MNC) isolated from umbilical cord blood or peripheral blood of individual donors. (promocell.com)
  • Primary Human Mononuclear Cells isolated from umbilical cord blood and peripheral blood (PBMC). (promocell.com)
  • The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily distinguished with specific markers. (lu.se)
  • COMT immunoreactivity was studied in primary astrocytic cultures from newborn rat cerebral cortex, and in neuronal cultures from rat brain from 18-day-old rat embryos using antisera against rat recombinant COMT made in guinea pig. (lu.se)
  • Critical for the development and production of biotherapeutic products, cell culture for manufacturing requires scalable products that are traceable and pure yet economically sound. (sigmaaldrich.com)
  • We offer the industry's highest quality sterile filtered liquid capabilities, supplying ready-to-use cell culture media, buffers, CIP and SIP products from GMP facilities worldwide to optimize your biopharma production. (sigmaaldrich.com)
  • Our custom cell culture product design experts can offer advice and support as you scale your bioprocess from clinical to commercial supply, assisting with liquid products, dry powder products, selection of raw materials, quality testing, flexible product packaging, and product documentation. (sigmaaldrich.com)
  • Researchers in Japan have replicated cancer cells from diseased bladder tissue in dogs, minimizing the use of costly stem cell products. (eurekalert.org)
  • Building on a long and expert history in cell culture, Corning offers viral vector manufacturing products, such as the HYPERFlask cell culture vessel to better advance your gene therapy applications. (corning.com)
  • In July 2019, several states "declared war" on advertising plant- or cell-based products as "meat. (provisioneronline.com)
  • The advantages of deriving active ingredients for cosmetics products from plant cell cultures were discussed at this year's congress, 'Plants for Human Health in the Post Genomic Era', held in Helsinki. (cosmeticsdesign-europe.com)
  • Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
  • At this point, the creator of the cell line must perform some form of authentication and characterization that identifies it as a unique cell line. (atcc.org)
  • Cell line development workflows consist of a multistep process involving single-cell cloning, formation and isolation of these discrete colonies, and the phenotypic characterization of these colonies. (moleculardevices.com)
  • When the final cell culture process is established, companies move forward with the commercialization process which includes process characterization, scale-up, technology transfer and manufacturing process validation. (beckman.com)
  • Yoshikawa S, Zen Y, Fujii T, Sato Y, Ohta T, Aoyagi Y, Nakanuma Y. Characterization of CD133 + parenchymal cells in the liver: Histology and culture. (wjgnet.com)
  • This includes regular disinfection of all workspaces, cell-culture storage areas and equipment (pipettes, laminar flow hoods, incubators, etc.) [2] . (invivogen.com)
  • Cannulation of SC with gelatin-coated suture material is an effective method for the isolation of human SC cells and provides a cellular model to study the potential role of SC cells in aqueous humor outflow function. (nih.gov)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
  • Cell culture stations are a portable solution for aspirating tissue culture samples. (thomassci.com)
  • C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
  • Incubation in tissue culture is 40-72 hours, depending on the cell type and specific biovar. (medscape.com)
  • Grow large quantities of adherent cells in a compact space with the Corning HYPERFlask cell culture vessel. (corning.com)
  • Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
  • Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)
  • Western blots detected both soluble 24 kDa and membrane-bound 28-kDa COMT proteins in neuronal and astrocyte cultures. (lu.se)
  • But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)
  • Upon completion of this course students should be able to demonstrate the knowledge and skills required to initiate, maintain, and manipulate cells in culture. (ncbionetwork.org)
  • Cell culture is integral to molecular and cellular biology research, and as such, proficient culturing techniques are essential to experimental success. (corning.com)
  • Once the cells have differentiated into the desired type, the cellular material can be harvested from the controlled environment and prepared using conventional food processing and packaging methods. (usda.gov)
  • Here, we highlight various applications and research methods using our CloneSelect Imager and Single-Cell Printer, ImageXpress Cellular Imaging System, and SpectraMax® microplate reader to help scale your cell line development workflow. (moleculardevices.com)
  • Traditionally, research on the replication of shrimp viruses has been based on the use of cultured fish cellular lines ( 6 ). (cdc.gov)
  • The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (cdc.gov)
  • Next, small cellular groups and some isolated round cells were observed. (cdc.gov)
  • The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (cdc.gov)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
  • Our ready-to-use, off-the-shelf cell line systems greatly reduce the time, cost, and risk of developing new cell lines for production. (sigmaaldrich.com)
  • Other important aspects of cell culture include 3D culture, engineering cell lines , and cell line authentication . (atcc.org)
  • While the authentication of human cell lines has been addressed with STR profiling, up until now the validation of mouse cell lines has been limited at the species level. (atcc.org)
  • Watch our on-demand webinar to explore the development of a new STR profiling method for the authentication of mouse cell lines. (atcc.org)
  • Misidentified and contaminated cell lines undermine your experimental results and discredit preclinical studies. (atcc.org)
  • Molecular Devices has a history of success in scaling biologics for regulatory approval and commercialization with life science groups worldwide, and can leverage that expertise with food manufacturers growing animal cell lines in new industries. (moleculardevices.com)
  • 2009, FASEB J). These cells were isolated as three independent immortal lines. (selectbiosciences.com)
  • Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (cdc.gov)
  • Control cell lines were injected with filtered hepatopancreas extracts from healthy shrimp. (cdc.gov)
  • Fresh cell lines were inoculated with the lysate, incubated, and processed in a similar way. (cdc.gov)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. (wikipedia.org)
  • This study is concerned with the detectionof overt and latent viruses in explant cultures of renal biopsies from 72 patients with various forms of renal disease. (karger.com)
  • This study confirms the absence of overt viruses in renal biopsy cultures. (karger.com)
  • Move stem cell therapy research forward with the HYPERFlask cell culture vessel from Corning. (corning.com)
  • Another part of the webinar series, this webinar covers tools and solutions that enable the advancement of stem cell therapy workflows to keep pushing the boundaries of what is possible. (corning.com)
  • Our experienced applications team is at the ready to work with you to ensure you have what you need to deliver viable cells and high yields in your stem cell therapy research. (corning.com)
  • A cloning and expression system to probe T-cell receptor specificity and assess functional avidity to neoantigens. (broadinstitute.org)
  • Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)
  • An anti-ageing active based on an extremely rare form of apple stem cells has been launched by Mibelle Biochemistry, promising to protect skin stem cells and slow the senescence of hair follicles. (cosmeticsdesign-europe.com)
  • In his search for effective therapies for Parkinson's disease, Lorenz Studer is uncovering pluripotency switches and clues to what makes cells age. (the-scientist.com)
  • The scaffold allowed the researchers to significantly expand T cell numbers in a dish, compared with existing culture methods, and could bring T cell therapies, such as anti-cancer treatments, closer to clinical reality. (medgadget.com)
  • Anti-cancer T cell therapies involve culturing and sometimes modifying patient-specific T cells in a dish before administering them to a cancer patient, where they can attack and kill cancer cells. (medgadget.com)
  • In the right SEM image, T cells (in blue) bind to a section of a completed antigen-presenting cell-mimetic scaffold (in brown), where they are instructed to multiply and are kept alive for future use in T cell therapies. (medgadget.com)
  • This webinar provides potential solutions that may be considered to improve cell density and productivity in adherent cell culture in the areas of vaccines and viral vector production. (corning.com)
  • However, because TSV could potentially represent a public health threat, we explored whether this viral agent might be capable of infecting cultured mammalian cells. (cdc.gov)
  • Using foetal cells from aborted embryos for transplantation to the brains of Parkinson patients is an avenue that has been explored by neuroscientists on and off for the last thirty years. (lu.se)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • Results: The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cell embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • Our wide range of advanced 3D cell culture scaffolds includes nanofiber, collagen, polystyrene, and polycaprolactone materials. (sigmaaldrich.com)
  • Why is collagen used in cell culture? (thermofisher.com)
  • When collagen solution is neutralized, it forms a gel that can support 3D cell cultures. (thermofisher.com)
  • Figure 1 shows Gibco liquid collagen for cell culture and collagen from two other suppliers. (thermofisher.com)
  • Gibco liquid collagen for cell culture, Supplier A collagen, and Supplier B collagen were plated into a 24-well plate at 3 mg and 6 mg concentrations. (thermofisher.com)
  • Ritty, T.M., Herzog, J. "Tendon cells produce gelatinases in response to type I collagen attachment. (thermofisher.com)
  • We have established a novel type of pluripotent neural crest-like stem cells (NCLSCs) from neonatal mouse epidermis (Sviderskaya et al. (selectbiosciences.com)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • The right culture vessel is crucial here, and different cell types may require different surfaces, such as plastic, glass, or coated surfaces. (coleparmer.com)
  • Subculturing, or passaging, is a critical part of banking and ensures the propagation of the cell line. (atcc.org)
  • To prevent this from occurring, the new cell line may be immortalized using techniques such as the vinyl chloride immortalization method or transfection with genes such as SV40 T antigen or human telomerase. (atcc.org)
  • Depending on whether the cell line is of human, mouse, or another animal short tandem repeat (STR) profiling or cytochrome oxidase (CO)1 barcoding can be used. (atcc.org)
  • Be a part of the movement to raise credibility in science and order your cell line authentication and mycoplasma testing services today. (atcc.org)
  • or buy on-line from www.bn.com or www.amazon.com Rush me _____copies of the Directory of Toll Fermentation and Cell Culture Facilities, Fourth Edition Name______________________________ Company___________________________ Address____________________________ _____________________________________ City_______________State_____Zip______ Country______________________ *Shipping: Domestic included in price. (bio.net)
  • In men, the best specimen for N gonorrhoeae culture is urethral exudate or urethral scrapings (obtained with a loop or special swab). (medscape.com)
  • During a vaginal examination, the health care provider uses a swab to take samples of mucus and cells from the endocervix. (medlineplus.gov)
  • In infants with suspected chlamydial pneumonia, perform a nasopharyngeal swab for Chlamydia culture. (medscape.com)
  • The Plant Cell Culture Library (PCCL) at UMass Amherst is a globally unique collection of callus cell cultures representing more than 1,000 diverse plant species. (umass.edu)