Plant Cells: Basic functional unit of plants.Vanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Taxus: Genus of coniferous yew trees or shrubs, several species of which have medicinal uses. Notable is the Pacific yew, Taxus brevifolia, which is used to make the anti-neoplastic drug taxol (PACLITAXEL).Petroselinum: A plant genus of the family APIACEAE used for flavoring food.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Quinine: An alkaloid derived from the bark of the cinchona tree. It is used as an antimalarial drug, and is the active ingredient in extracts of the cinchona that have been used for that purpose since before 1633. Quinine is also a mild antipyretic and analgesic and has been used in common cold preparations for that purpose. It was used commonly and as a bitter and flavoring agent, and is still useful for the treatment of babesiosis. Quinine is also useful in some muscular disorders, especially nocturnal leg cramps and myotonia congenita, because of its direct effects on muscle membrane and sodium channels. The mechanisms of its antimalarial effects are not well understood.Existentialism: Philosophy based on the analysis of the individual's existence in the world which holds that human existence cannot be completely described in scientific terms. Existentialism also stresses the freedom and responsibility of the individual as well as the uniqueness of religious and ethical experiences and the analysis of subjective phenomena such as anxiety, guilt, and suffering. (APA, Thesaurus of Psychological Index Terms, 8th ed.)Holistic Health: Health as viewed from the perspective that humans and other organisms function as complete, integrated units rather than as aggregates of separate parts.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Great BritainComputer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.PaperTime Factors: Elements of limited time intervals, contributing to particular results or situations.Maze Learning: Learning the correct route through a maze to obtain reinforcement. It is used for human or animal populations. (Thesaurus of Psychological Index Terms, 6th ed)Curriculum: A course of study offered by an educational institution.Problem-Based Learning: Instructional use of examples or cases to teach using problem-solving skills and critical thinking.

The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)

The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen.  (+info)

In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)

Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (6/155046)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

*Cell culture

Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Microcarrier Cell ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... Culture of human stem cells is used to expand the number of cells and differentiate the cells into various somatic cell types ...

*3D cell culture

Cell culture Cell lines Cell culture assay Madin-Darby Canine Kidney cell line Hydrogel Fey, Stephen; Wrzesinski, Krzysztof ( ... cell-cell/cell-matrix interaction, and/or cell migration, but the available assays are not optimized for 3D cell culturing. The ... Lastly, 3D cell cultures have greater stability and longer lifespans than cell cultures in 2D. This means that they are more ... Furthermore, 3D cell cultures provide more accurate depiction of cell polarization, since in 2D, the cells can only be ...

*Spinner (cell culture)

Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment ... usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass ...

*Microfluidic cell culture

"Versatile, Fully Automated, Microfluidic Cell Culture System" (PDF). Versatile, Fully Automated, Microfluidic Cell Culture ... rapidly in microfluidic culture.Non-dividing or slowly dividing cells can be culture continuously in the same cell culture ... store.elsevier.com/Microfluidic-Cell-Culture-Systems/isbn-9781437734591/ "Microfluidic cell culture" (PDF). ScienceDirect. ... Microfluidic cell culture attempts to develop devices and techniques of culturing, maintaining, analyzing and experimenting in ...

*Cell culture assay

A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media is removed The cells are covered ... A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media ([agar] generally) is removed ... Cell culture evaluations are the precursor to whole animal studies and are a way to determine if significant cytotoxicity ... In biomaterials testing, a cell culture assay is any method which is used to assess the cytotoxicity of a material. This refers ...

*Insect cell culture

... this mechanism is not present in prokaryotic system The Sf9 is one of the most commonly used cell lines in insect cell culture ... Insects cell lines are used because post translational modifications of protein are possible in insect cells whereas ... The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are ... Drugmand, Jean-Christophe; Schneider, Yves-Jacques; Agathos, Spiros N (2011). "Insect Cells as Factories for Biomanufacturing ...

*Rotary Cell Culture System

The cell culture bioreactor-known commercially as the Rotary Cell Culture System (RCCS) -- boasts several advantages that ... Lab-grown cell cultures tend to be small, flat and two-dimensional, unlike normal cultures. But tissues grown in the RCCS are ... The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. In the ... A closed tubular cylinder forms the system's cell culture chamber, which is filled with a liquid medium in which cells grow on ...

*European Collection of Authenticated Cell Cultures

The European Collection of Authenticated Cell Cultures houses and supplies cell lines. It is part of the Culture Collections of ...

*3D cell culturing by magnetic levitation

3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high- ... Because of the ability of 3D cell culturing by magnetic levitation to bring cells together, co-culturing different cell types ... Standard monolayer cell culturing on tissue culture plastic has notably improved our understanding of basic cell biology, but ... Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio- ...

*3D cell culture in wood-based nanocellulose hydrogel

Different cell lines and cell types have been cultured in NFC, including e.g. differentiation of human hepatic cells to ... like cell culturing. GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. NFC fiber network structure ... functional organotypic cultures, and proliferation of human pluripotent stem cells. Organotypic liver cell cultures can be used ... Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. As plant based material, it ...

*Stable isotope labeling by amino acids in cell culture

Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing ... While SILAC had been mostly used in studying eukaryotic cells and cell cultures, it had been recently employed in bacteria and ... SILAC (stable isotope labeling by/with amino acids in cell culture) is a technique based on mass spectrometry that detects ... Amanchy R, Kalume DE, Pandey A (2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying dynamics ...

*Hybridoma technology

The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... These isolated B cells are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a ... the B cells are fused with immortalised myeloma cells. The fusion of the B cells with myeloma cells can be done using ... These cells produce antibodies (a property of B cells) and are immortal (a property of myeloma cells). The incubated medium is ...

*Free-living Amoebozoa infection

... mammalian cell cultures; vegetables; human nostrils and throats; and human and animal brain, skin, and lung tissues. B. ...

*Timeline of typhus

Maramorosch, Karl; McIntosh, Arthur H. (1994-06-03). Arthropod Cell Culture Systems. ISBN 9780849376429. Retrieved 4 February ...

*Perfluorodecalin

Due to its gas-carrying capacity, perfluorodecalin has been used to enhance oxygen delivery during cell culture. ... King, A. T.; Mulligan, B. J.; Lowe, K. C. (1989). "Perfluorochemicals and Cell Culture". Nature Biotechnology. 7: 1037-1042. ... Artificial cells, blood substitutes, and immobilization biotechnology. 33 (1): 37-46. PMID 15768564. ...

*Fine chemical

Commonly used cell lines are Chinese hamster ovary (CHO) cells or plant cell cultures. The production volumes are very small. ... When carried out outside the natural habitat, the process is called cell culture. Mammalian cell culture fermentation, also ... Cell Cultures Animal or plant cells, removed from tissues, will continue to grow if cultivated under the appropriate nutrients ... Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells. Humana Press, New York. C. Chassin; P. Pollak ( ...

*Cell-based vaccine

The potential use of cell culture techniques in developing viral vaccines, especially for the Influenza virus, has been widely ... The vaccine was produced by Novartis through culturing of the Madin-Darby canine kidney (MDCK) cell line. Specifically, ... "Is Egg-based Vaccine Manufacturing on its Way Out?". The Cell Culture Dish. Retrieved October 9, 2017. Toovey, Stephen (2007 ... Doroshenko A, Halperin S. Trivalent MDCK cell culture-derived influenza vaccine Optaflu (Novartis Vaccines). Expert Reviews. ...

*Vidarabine

I. Cell Culture Studies, ibid., 1968, 8, 136-147. Komura, H.; Yoshino, T.; Ishido, Y. An Easy Method of Preparing Cyclic ... is three times longer in HSV-infected cells compared with uninfected cells, however the mechanism of selectivity is not known. ... and the isolation of Vidarabine from the fermentation culture broth of Streptomyces antibioticus. In addition to the potential ... Inhibitory Effect of Some Nucleosides on the Growth of Various Human Viruses in Tissue Culture, Antimicrobial Agents and ...

*Budapest Treaty

... plant or animal cell cultures; yeast, algae, protozoa, eukaryotic cells, cell lines, hybridomas, viruses, plant tissue cells, ... cells, for example, bacteria, fungi, eukaryotic cell lines, plant spores; genetic vectors (such as plasmids or bacteriophage ... World Federation for Culture Collections (WFCC) National Collection of Type Cultures (NCTC) WIPO web site, Contracting Parties ... spores, and hosts containing materials such as vectors, cell organelles, plasmids, DNA, RNA, genes and chromosomes; purified ...

*Ptk2 cells

Atlas of Living Cell Cultures. Weinheim: Wiley. p. 465. ISBN 3527669930. "Olympus Microscopy Resource Center , Fluorescence ... PtK2 Cells are a cell line derived from male rat-kangaroo (Potorous tridactylis) epithelial kidney cells. This cell line is ... PtK2 cells are relatively large and when grown in a monolayer stay flat throughout the cell cycle unlike many cells that round ... Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of ...

*Exometabolomics

Cuperlovic-Culf, Miroslava (2014). "Metabolomics in Animal Cell Culture". In Al-Rubeai, M. Animal Cell Culture. Springer ... uninoculated/control culture media; this approach can differentiate different physiological states of wild-type yeast and ... Recently, exometabolomics has been used to design co-culture systems. Because the analysis of extracellular metabolites allows ... Silva, Leslie; Northen, Trent (2015). "Exometabolomics and MSI: deconstructing how cells interact to transform their small ...

*Katarína Horáková

Horáková, K (1999). "The use of cell culture systems for the assessment of general cellular toxicity and to detect the nature ... Horáková, K. (1981). "The Use of Cell Cultures to Monitor the Toxic and Mutagenic Activity of Chemical Compounds Present in ... Horáková, K (1981). "Carcinogenic potential in cell cultures". Československá fysiologie. 30 (6): 517-25. PMID 7032713. ... Jantovà, S.; Horàkovà, K. (1993). "9-Hydroxybenfluron induced inhibition of proliferation and metabolism in hela cells". Cell ...

*Morindone

by Root Cell Culture" (PDF). Chiang Mai University Journal. 1 (1): 66-78. Retrieved 2009-01-05. "Morinda citrifolia". World ... Recent research has examined cell culture as a means of increasing yields. "Morindone". NCBI PubChem. Retrieved 2008-12-27. ... some attempts have been made to speed up production using tissue cultures. Morindone requires a mordant, and the color obtained ...

*Karl Maramorosch

Arthropod Cell culture Systems, 1994. Forest Trees and Palms- Diseases and Control, 1996. Invertebrate Cell Culture-Novel ... and mammalian cell gene delivery. The Mitsuhashi-Maramorosch insect culture medium for culturing insect cells is now a widely ... Advances in Cell Culture, 7 vols., 1981-1989. 57-215. Advances in Virus Research, 56 vols. 1973-2015. 1949-60: Assistant to ... Since 1956, when Maramorosch first cultured insect cells for use in the study of viruses, he has been an active contributor to ...

*Verbascoside

"Verbascoside production by plant cell cultures". Plant Cell Reports. 9 (9). doi:10.1007/BF00232101. Wysokiińska, H; Rózga, M ( ... It can also be produced in plant cell cultures of Leucosceptrum sp (Lamiaceae) and Syringa sp (Oleaceae). It can also be ... 1998). "Establishment of transformed root cultures of Paulownia tomentosa for verbascoside production". Journal of Plant ... produced in hairy roots cultures of Paulownia tomentosa (empress tree, Paulowniaceae). Verbascoside has an antimicrobial ...

*STS-47

Test subjects included the crew, Japanese koi fish (carp), cultured animal and plant cells, chicken embryos, fruit flies, fungi ... Life sciences included experiments on human health, cell separation and biology, developmental biology, animal and human ...
Langford test 1 (from quizlet) - infectious diseases 4180 with at muscles. Human Muscle Cell Can Perform Quizlet pleasant in order to the web site, within this moment I will explain to you with regards to Human muscle cell can perform quizlet.. And from now on, this can be the very first picture, human muscle cells can perform quizlet :. ...
The present study was undertaken to elucidate different requirements for CD2-mediated activation of naive (CD45RO-) and memory (CD45RO+) CD4+ T cells. A mitogenic combination of anti-CD2 (anti-T11(2) and anti-T11(3] mAbs could effectively induce the proliferation of memory CD4+ T cells even in the absence of monocytes. In marked contrast, naive CD4+ T cells did not disclose any proliferative responses to anti-CD2 mAbs, when monocytes were absent in culture. This differential responsiveness of naive and memory CD4+ T cells appeared to be related largely to a difference in IL-6-producing ability between both populations. IL-6 among monocyte-derived cytokines could correct unresponsiveness of naive CD4+ T cells to anti-CD2 stimulation. Unlike naive CD4+ T cells, memory CD4+ T cells produced IL-6 by themselves, with its mRNA being expressed on anti-CD2 stimulation. Anti-IL-6R mAb significantly inhibited proliferation of memory CD4+ T cells seen in the anti-CD2-stimulated cultures without monocytes, ...
TLR3 recognizes double-stranded RNA, a product associated with viral infections. Many details of TLR3-induced mechanisms have emerged from gene-targeted mice or inhibition studies in transformed cell lines. However, the pathways activated in human immune cells or cells from disease tissue are less well understood. We have investigated TLR3-induced mechanisms of human primary cells of the innate immune system, including dendritic cells (DCs), macrophages (MØs), endothelial cells (ECs), and synovial fibroblasts isolated from rheumatoid arthritis joint tissue (RA-SFs). Here, we report that while these cells all express TLR3, they differ substantially in their response to TLR3 stimulation. The key antiviral response chemokine IP-10 was produced by all cell types, while DCs and MØs failed to produce the proinflammatory cytokines TNFalpha and IL-6. Unexpectedly, TNFalpha was found secreted by TLR3-stimulated RA-SF. Furthermore, TLR3 stimulation did not activate NFkappaB, MAPKs, or IRF-3 in DCs and ...
During atherogenesis, EC/SMC cross-communication via secreted paracrine factors is proposed to promote the chronic inflammatory response localized to hemodynamically compromised regions of arteries. As shown previously, VCAM-1 is expressed in human SMCs in proximity to human ECs exposed to atheroprone flow3 and IL-8 was upregulated and secreted at higher levels by ECs exposed to atheroprone flow.3 The functional role of IL-8 in regulating vascular cell phenotype is unknown. A major challenge for the field is that the cytokine IL-8 is not expressed in rodents. Thus, we used a model that recalibrates human in vivo vascular cell phenotypes in vitro to unmask a novel role for IL-8 in regulating SMC phenotype. Herein, we show that IL-8 attenuates a proinflammatory SMC phenotype by reducing VCAM-1 expression via CXCR2, p38, and NF-κB mechanisms. We also demonstrate that atheroprone flow induces VCAM-1-dependent monocyte adhesion.. The proinflammatory phenotype promoted during atheroprone flow ...
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Microbiology culture preparation. Person using a bunsen flame to sterilise the top of a jar containing a bacterial culture. - Stock Image C024/5723
Growth Requirements. The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. To obtain best growth, addition of a small amount of blood serum is usually necessary, and several antibiotics, like penicillin and streptomycin are added to prevent bacterial contamination.. Temperature varies on the type of host cell. Most mammalian cells are maintained at 37oC for optimal growth, while cells derived from cold- blooded animals tolerate a wider temperature range (i.e. 15oC to 26oC). Actively growing cells of log phage should be used which divide rapidly during culture.. Process to obtain primary cell culture. Primary cell cultures are prepared from fresh tissues. Pieces of tissues from the organ are removed aseptically; which are ...
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
CellSeed, in Japan, is developing a series of cell therapies based on its regenerated human cell culture technology (UpCell®) for the potential treatment of
Ongoing advances in quantitative molecular- and cellular-biology highlight the need for correspondingly quantitative methods in tissue-biology, in which the presence and activity of specific cell-subpopulations can be assessed in situ. However, many experimental techniques disturb the natural tissue balance, making it difficult to draw realistic conclusions concerning in situ cell behaviour. In this study, we present a widely applicable and minimally invasive method which combines fluorescence cell labelling, retrospective image analysis and mathematical data processing to detect the presence and activity of cell subpopulations, using adhesion patterns in STRO-1 immunoselected human mesenchymal populations and the homogeneous osteoblast-like MG63 continuous cell line as an illustration.. Adhesion is considered on tissue culture plastic and fibronectin surfaces, using cell area as a readily obtainable and individual cell specific measure of spreading. The underlying statistical distributions of ...
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
Splitting A549 cells - posted in Tissue and Cell Culture: I am new to cell cultures, and I have been assigned the task of splitting someones A549 cells.. I have a T75 flask that I was told to split into 5 flasks, and add 30 mL of media to each. I just wanted to verify something-if I split 1:5, I re-suspend the cell pellet in 5 mL of media and add 1 ml of the cell/media solution to each of the flasks? I am trying to properly grasp the concept of splitting cells in certain ratios, so i...
8. This allows further bacterial multi- plication topama subsequent production of an increased amount of inflammatory mediators via the complement system and other pathways.
A self-contained mini-greenhouse useful for propagating cuttings, germinating seeds, and acclimating tissue-cultured plants intended for transfer to soil. Includes plastic tray …
Study Flashcards On PA 2008- Patho ch. 2 at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Study Flashcards On week 3 patho at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Homogeneous cell populations in less than an hour. Manual and automated sorting, scalable to serve your needs. No need for transgenic selection markers.
The human system, critical infrastructure, environment, human society, human etc. are represented by system of systems, i.e. they consist...
Cell culture media and reagents for the stimulation, expansion, and differentiation of human and mouse primary cells, including immune cells, stem cells, and neural cells. - Deutschland
هدف: سلول‌های بنیادی پرتوان مشتق شده از بافت بیضه، یک منبع بسیار غنی و جدیدی برای سلول درمانی در پزشکی بازساختی هستند. مطالعات اخیر نشانگر آن است که سلول‌های بنیادی اسپرماتوگونی در شرایط آزمایشگاهی می‌توانند به سلول‌های بنیادی شبه جنینی تغییر یابند.استفاده از امواج فراصوت با شدت پایین، آثار مثبتی روی رشد و تمایز سلول‌ها دارد. هدف از این مطالعه تأثیر امواج فراصوت روی کلونی‌زایی سلول‌های بنیادی شبه جنینی است. مواد و روش‌ها: ابتدا، سلول‌های بنیادی اسپرماتوگونی از بیضه نوزاد موش جدا شدند. بعد از کشت دادن این سلول‌ها در محیط DMEM/F12، حاوی 15 درصد سرم جنین
Researchers have found that by putting one or two mutations into the H5N1 bird-flu virus, they were able to give it greater ability to slip into human cells. The new information will give virus-trackers something to watch for as H5N1 expands to new territory.
The purpose of the present investigation was to explore the effects of well-defined flow conditions on the activity of tissue factor (TF) expressed on the surface of cultured rat vascular smooth muscle cells. Cells were cultured to confluence on Permanox brand slides and stimulated to express TF by a 90 min incubation with fresh growth medium containing 10 percent calf serum. The stimulated cells were then placed in a parallel plate flow chamber and perfused with Hanks Balanced Salt Solution containing factor VIIa, factor X (FX), and calcium. The chamber effluent was collected and assayed for factor Xa (FXa) and the steady-state flux of FXa was calculated. The flux values were 68.73, 94.81, 139.75, 138.19, 316.82, and 592.92 fmole/min/cm2 at wall shear rates of 10, 20, 40, 80, 320, and 1280 s−1 respectively. The FXa flux depended on the wall shear rate to a greater degree than predicted by classical mass transport theory. The flux at each shear rate was three to five times less than that ...
I am planning to set up a co-culture system for human endothelial cells and human VASCULAR smooth muscle cells. I would be really interested to find out about methods of extraction and primary culture of human vascular smooth muscle cells, if anyone can help and advise, please mail me! Thanks Pippa Deex ...
Vascular smooth muscle (VSM) cell proliferation contributes to the pathogenesis of atherosclerosis, restenosis after angioplasty and vein graft disease. The regulation of genes involved in VSM cell proliferation, particularly by naturally occurring inhibitors, is therefore of some importance. We have investigated the role of the c-myc proto-oncogene in growth arrest of exponentially proliferating rat VSM cells, following mitogen withdrawal, treatment with heparin (50 micrograms/ml), interferon-gamma (IFN-gamma) (100 i.u./ml), or the cyclic nucleotide analogues, 8-bromo-adenosine-3′5′-cyclic monophosphate (8-Br-cAMP; 0.1 mM) and 8-bromoguanosine-3′5′-cyclic monophosphate (8-Br-cGMP; 0.1 mM). Growth arrest was accompanied by down-regulation of c-Myc protein and mRNA following treatment with all inhibitors. Serum withdrawal or IFN-gamma treatment suppressed c-myc expression by more than 50% within 2 h, and this occurred throughout the cell cycle. Platelet-derived growth factor, epidermal ...
Oral administration of rats to L-2-chloropropionate (L-CPA) causes selective necrosis to the granule cell layer of the cerebellum in vivo and to cultured rat cerebellar granule cells in vitro. The present study was conducted to characterize the involvement of reactive oxygen species (ROS) in cell death of L-CPA to rat cerebellar granule cells in vitro. Exposure to L-CPA (0.625-10 mM) produced a concentration dependent increase in formation of 2,7-dichlorofluorescein (DCF) as a measure of formation of ROS. The elevation of ROS was inhibited after incubation of the cells with the ERK-type of MAP kinases inhibitor U0126, the mitochondrial permeability transition pore inhibitor cyclosporin A (CSA), the antioxidant vitamin E, and the spin trap N-tert-butyl-α-(2-sulfophenyl)-nitrone (S-PBN). Measurements of nitrite (NO2) in the cell culture supernatant using the Griess reagent indicate generation of nitric oxide (NO) after exposure to L-CPA. Incubation with L-CPA (10 mM) for 48 hr lead to cell death ...
The concept of arterial SMC heterogeneity has gained wide acceptance in the last years.1 2 33 The distinct phenotypes of arterial SMCs have been mainly identified in vitro,4 5 6 7 8 9 10 11 12 13 14 15 16 suggesting that specific features of SMC populations arise and are maintained in the particular environment of cell culture. Hence, it was of interest to investigate whether in vitro SMC phenotypes are preserved when SMCs are placed back in an in vivo environment. For this purpose, we have implanted 2 SMC populations exhibiting distinct levels of differentiation in vitro into the rat carotid artery submitted to endothelial injury.24 25 The implanted SMCs were marked with PKH-26, a lipophilic cell membrane linker that is halved with each cell division but is not lost from the cell membrane.34 Our results show that the 2 implanted populations essentially retain for 20 days in vivo the phenotype that they specifically exhibited in vitro.. Spindle-shaped and epithelioid rat SMC populations have ...
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Jochen Meier Ivonne Strmel Radu Iosub Sonja Schmidt and Rosemarie Grantyn Developmental Physiology Johannes Mller Institute Humboldt University Medical School (Charit) Berlin Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance,Effectene,Transfection,Reagent,provides,efficient,gene,delivery,,,,,,,,,,,,to,primary,neuronal,cell,cultures,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
The Establishment of a Primary Culture System of Proximal Tubule Segments Using Specific Markers from Normal Mouse Kidneys. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Jacobs, B B., "In vivo assay of function of mouse ovaries following culture in hormone enriched medium." (1963). Subject Strain Bibliography 1963. 546 ...
Many studies have shown that TRAIL is a potent apoptosis inducer in malignant cells, whereas its role in normal cell physiology is much less well understood.1 We have demonstrated here that both aortic endothelial cells and HUVECs exhibit a similar pattern of surface TRAIL-R expression. Moreover, in vascular endothelial cells, TRAIL stimulates the phosphorylation of the serine/threonine kinase Akt in a manner dependent on PI3K activation.14 The ability of TRAIL to activate the antiapoptotic PI3K/Akt pathway in endothelial cells is a completely new and unexpected finding, also in consideration of the large number of studies underlining the proapoptotic activity of TRAIL, at least in malignant cells.1,4,5 It should be underlined that the PI3K/Akt pathway is of central importance in endothelial cell biology, conferring survival to endothelial cells in response to angiogenic cytokine stimulation, fluid shear stress, and matrix attachment signals. This pathway is essential also for endothelial cell ...
TY - JOUR. T1 - Activation of caspase-8 and Erk-1/2 in domes regulates cell death induced by confluence in MDCK cells. AU - Chang, Yung Heng. AU - Lin, Hsi Hui. AU - Wang, Yang Kao. AU - Chiu, Wen Tai. AU - Su, Hsiao Wen. AU - Tang, Ming Jer. PY - 2007/4/1. Y1 - 2007/4/1. N2 - Under normal culture conditions, cells adhere to culture dish, spread out, proliferate, and finally cover all areas and reach confluence. During the confluent stage, cell proliferation ceases and differentiation is enhanced. Meanwhile, cell death also appears as the monolayer confluence proceeds. To delineate the mechanism of cell death induced by the confluent process, we employed Madin-Darby canine kidney (MDCK) cells. When approaching confluence, MDCK cells exhibited increase the levels of caspase-2 and enhanced the activity of caspase-8. Using various caspase inhibitors to block apoptosis, we found that only z-VAD-fmk and z-IETD-fmk can inhibit confluent cell death, indicating that confluent cell death is mediated by ...
In the present article, we compared the antiangiogenic activity of trans- and cis-resveratrol on endothelial cells of different origin activated by endogenous or exogenous stimuli. The results indicate that trans-resveratrol affects endothelial cell proliferation and various cell proliferation-independent aspects of the angiogenesis process, including endothelial cell sprout formation, collagen gel invasion, and morphogenesis. Thus, trans-resveratrol acts as an angiostatic molecule on multiple targets of the angiogenic process. In all the assays, the cis stereoisomer exerted only a limited effect, if any. In keeping with the in vitro observations, trans-resveratrol was more effective than the cis isomer in inhibiting angiogenesis and tumor growth in vivo.. The molecular bases of the antiangiogenic activity of resveratrol are poorly understood (16). trans-Resveratrol is endowed with a significant antioxidant and anti-inflammatory activity (8, 9). However, no direct relationship exists between the ...
hi, i am first time in forum,. my concern is,. can serum free medium inactivate trypsin?? i didnot have this idea so mistakenly i used serum free medium to inactivate trypsin which resulted transfected cells(gfp +ve) lost gfp(confirmed by florescence microscope) but still cells are alive. can anyone help pls.. ...
The effects of interferons (IFNs) on lymphocyte adhesion to cultured human vascular endothelial cells (EC) were investigated using an in vitro assay. Endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (CM) from 12-O-tetra decanoylphorbol 13-acetate-concanavalin A (TPA-Con A) stimulated human peripheral blood lymphocytes (PBL), or with recombinant (r) gamma interferon (IFN-gamma) or r alpha interferon (IFN-alpha), and then were incubated with freshly isolated PBL. Natural IFN-gamma in the TPA-Con A CM and rIFN-gamma (12.5-500 U/ml) induced major histocompatibility complex-class II antigens (HLA-DR, HLA-DP, and HLA-DQ) and significant lymphocyte adhesion to the EC, whereas rIFN-alpha did not. The lymphocyte adhesion to the EC and the expression of DR antigens on the EC were well correlated in terms of both kinetics and the dose-response pattern of rIFN-gamma. When EC expressing I region associated (Ia) antigen were preincubated with ...
The effects of interferons (IFNs) on lymphocyte adhesion to cultured human vascular endothelial cells (EC) were investigated using an in vitro assay. Endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (CM) from 12-O-tetra decanoylphorbol 13-acetate-concanavalin A (TPA-Con A) stimulated human peripheral blood lymphocytes (PBL), or with recombinant (r) gamma interferon (IFN-gamma) or r alpha interferon (IFN-alpha), and then were incubated with freshly isolated PBL. Natural IFN-gamma in the TPA-Con A CM and rIFN-gamma (12.5-500 U/ml) induced major histocompatibility complex-class II antigens (HLA-DR, HLA-DP, and HLA-DQ) and significant lymphocyte adhesion to the EC, whereas rIFN-alpha did not. The lymphocyte adhesion to the EC and the expression of DR antigens on the EC were well correlated in terms of both kinetics and the dose-response pattern of rIFN-gamma. When EC expressing I region associated (Ia) antigen were preincubated with ...
The fully sequenced pCERI is transformed into E.coli BL21 and E.coli Nissle 1917 that are used for the characterization of our regulatory system. The expression efficiency of the genes of interest is characterized by a fluorescence-based assay (Results) Overnight cultures of both recombinant strains are inoculated with single colonies. The overnight cultures are diluted with LB-Amp to an OD of 0.02. 200 μ of the dilutions are pipetted in microtiter plates and incubated at 37 °C in the plate reader. Every 15 minutes mRFP fluorescence (Ex. 584 nm, Em. 607 nm), CFP fluorescence (Ex. 439 nm, Em. 476) and OD600 is measured over a period of 13 h. The measured RFU (relative fluorescence unit) is either blotted against time or against OD600 to evaluate the expression behavior of pCERI. Additionally several samples are either induced with 0.1 -50 nM of 3OC6-HSL and 0.1-100 nM of C8-HSL to explore putative advantages of higher HSL-synthesis, that might be possible by positioning each HSL-synthase, ...
This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for ...
Background: Hepatocellular carcinoma (HCC), is characterized by severe vessel anomalies with an intense arterial blood supply, and acquisition of a basal membrane rich in laminin by sinusoids. A role of the VEGF-A/Dll4-Notch4/ephrin B2 cascade in tumor vessel anomalies has been demonstrated, raising the question of the nature of the VEGF receptor pathway implicated in triggering this cascade. This study was undertaken to determinate the role of PlGF and its receptor VEGFR-1 in the development of vascular anomalies in HCC and to evaluate the role of the downstream Notch4 pathway.. Material and Methods: In vitro, we used primary culture of HUVECs, isolated in our laboratory from human umbilical cords. HUVECs were cultured in EBM2 medium, with 20% of FBS and 2ng/ml of FGF-2. The expression of the active form of Notch4 and Dll4 in HUVECs were assessed by Western Blot. In vivo, the differential expression of VEGF-A, PlGF and their receptors were evaluated by qRT-PCR and immunostaining in transgenic ...
I am studying the differentiation of leukemic cells along the monocytic lineage using calcitriol as differentiative agent and others vitamins as possible stimulating compounds on differentiation. In my work I have used HL-60 cells as in vitro culture cells but now I have some problems because these cells have changed their response and develop expontaneous differentiation in normal culture conditions. Now, I am thinking on NB4 cells as a better cell line to study differentiation. I have ask some information to cell banks as ATCC and ECA but they dont have this cell line. Could anybody tell me if anyone can send me this cell line?, I have very gratefull if anyone could send me HL-60 cells too. Waiting positive responses and acknowledging in advance. Guillermo Lopez-Lluch Departamento de Biología Celular Facultad de Ciencias Universidad de Córdoba Avda. S. Alberto Magno S/N E-14007 Córdoba, Spain. TFNO: Int + 34-57-218634 FAX: Int + 34-57-218634 e-mail: bc2biced at lucano.uco.es ...
The MCF 10 cell line is a non-tumorigenic epithelial cell line. The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF 10 was derived from adherent cells in the population. Cells derived from a floating population are available. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera endotoxin, and epidermal growth factor (EGF). By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. The calcium content of the medium exerts a strong effect on the morphology of the cells ...
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One group of projects in the lab concerns "co-receptors", cell-surface molecules that regulate the interaction between soluble growth factors and their cell-surface receptors. We have identified a novel co-receptor for BMPs (bone morphogenetic proteins) and are studying the roles of Heparan Sulfate Proteoglycans (HSPGs) as co-receptors for a variety of growth factors, including those that drive tumor development and pattern the developing nervous system. We have used "knockout" technology to generate mice that are mutant for particular HSPGs, and are also working with mice that are mutant for some of the biosynthetic enzymes required to make HSPGs. We are also using modeling approaches to understand how HSPGs influences the kinetic parameters of growth factor-receptor interaction ...
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Tissue culture. SC cultures were prepared from neonatal rat sciatic nerves according to the methods of Brockes et al. (1979) and modified as described previously (Pareek et al., 1993). SC were maintained in medium supplemented with 5 μmforskolin to stimulate myelin-related gene expression (Lemke and Chao, 1988; Pareek et al., 1993).. Dissociated cultures of sensory neurons from Sprague Dawley rat embryos were established as described previously (Kleitman et al., 1991). Briefly, embryonic day 15 dorsal root ganglia (DRG) were enzymatically and mechanically dissociated. Cells were plated on 12 mm collagen-coated glass coverslips for morphological studies or on collagen-coated tissue culture plastic for biochemical analysis. The day after the dissection, the cultures were treated with fluorodeoxyuridine (10 μm) for at least three cycles to remove non-neuronal cells.. Co-cultures of DRG neurons and SC (DRG-SC) were established as described by Einheber et al. (1993). Briefly, SC were harvested by ...
Recent evidence supports a strong role for immune suppression in facilitating cancer progression. Transforming growth factor beta (TGFß) regulates a diverse array of biological events, including neoangiogenesis, stromal formation, and immune responses. Both TGFß and a small molecule inhibitor of TGFßR-1, LY364947, were effective regulators of these processes in vitro. Using quantitative RT-PCR and fluorescence imaging, TGFß increased collagen production by pancreatic stellate cells while addition of LY364947 suppressed production below basal levels. Endothelial cell proliferation, measured as a function of endothelial tube formation in Geltrex® matrix, was similarly increased by TGFß and reduced to basal levels by LY364947. To explore the in vivo effects of these molecules, LY364947 was encapsulated in liposomes to encourage tumor accumulation and to avoid administration of DMSO vehicle. Liposomes were prepared with a molar ratio of 6:3:1 1,2-dipalmitoyl-sn-glycero-3-phosphocholine ...
Conventional biology expects the process of mammalian cell division, mitosis, to occur by the equal partition of a mother cell into two daughter cells. Bioengineers at UCLA Engineering have developed a platform that mechanically ...
A growing body of evidence suggests that transiently activated NF-κB in noninflammatory states and persistently activated NF-κB during inflammation play different pathophysiological roles in vivo. During the initial phase of inflammation, proinflammatory cytokines and mediators induce prolonged NF-κB activation in various inflammatory cells and endothelium. The activated NF-κB further upregulates the expression of several proinflammatory molecules, such as endothelial cell adhesion molecules and macrophage inflammatory protein-2, which triggers neutrophilic infiltration and tissue injury (1, 27, 34). Thus persistent NF-κB activation during the early phase of inflammation amplifies inflammatory response in vivo.. In contrast, transient activation of NF-κB before inflammatory stimulation results in the anti-inflammatory response. For example, several investigators (41, 54, 56) have found that transient activation of NF-κB is required for the heart to tolerate ischemia-reperfusion-induced ...
Time-dependent induction of connected cells by overexpression of cdc5ΔN. (A) Strain KLY1083 expressing three copies of GAL1-EGFP-cdc5ΔN homogeneously induced
floating THP-1 cells - medium change advice - posted in Tissue and Cell Culture: Hello, I was adamantly asked to culture THP-1 cells and did so, by thawing a cryovial and seeding the cells in a 25 cm flask. 3 days later 99% of cells are floating, which I reckon is OK. Isnt it? So, do I need to change the medium before they reach ~8x 10^5 cells/ml and are ready to be passed? Since these are floating cells, how do I do that? Also, would a 1/3 split ratio be OK for these cells? Th...
1) The main issue with this paper is this: these cell lines, although originally human, are all immortalized cancer cell lines characterised by markedly different biological properties when compared to normal human cells of the same tissue/cell type. They cant be readily used a surrogates for normal human tissue/cell types. None were primary cells nor were any even from recently acquired tissue samples from biopsies etc. People have infected primary human airway epithelial cultures with hCoV-EMC - so this can be done successfully - , although it would be more difficult for other tissue types as these cultures havent been developed. Some of these cell lines used may by chance lack key viral repressors of infection present in normal primary cells, which could skew results from cell culture infection experiments. Plus, a human tissue is not just a single cell type - they are composed of diverse kinds of cells that could together behave much, much differently than cell lines in culture ...
Accepted name: raucaffricine β-glucosidase. Reaction: raucaffricine + H2O = D-glucose + vomilenine. For diagram click here.. Other name(s): raucaffricine β-D-glucosidase; raucaffricine glucosidase. Systematic name: raucaffricine β-D-glucohydrolase. Comments: Highly specific; some other ajmalan glucoside alkaloids are hydrolysed, but more slowly.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 102925-37-1. References:. 1. Schübel, H., Stöckigt, J., Feicht, R. and Simon, H. Partial-purification and characterization of raucaffricine β-D-glucosidase from plant cell-suspension cultures of Rauwolfia serpentina benth. Helv. Chim. Acta 69 (1986) 538-547.. ...
Finally, our article on human and mouse neurons has been published! The picture above shows the neurons of a human and a mouse. Human and mouse neurons are similar overall, but they also have…
CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics ...
483279889 - EP 3108256 A4 2017-10-04 - METHOD OF DETECTING CANCER - [origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.[origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.
Results ICAM-1 expression was significantly up-regulated upon the introduction of TNF-α under all conditions in HUVEC (figure 1). However, baseline expression was increased when co-cultured with both NHDF (2.0 vs 1.3, p,0.001) and HUASMC (6.5 vs 1.3, p,0.001). This meant that ICAM-1 expression at 12 hours was also significantly higher in co-culture with NHDF (8.3 vs 5.2, p,0.001) and HUASMC (11.0 vs 5.2, p,0.001). Moreover, there was a moderate relationship between HUVEC ICAM-1 expression and the cell ratio when in co-culture with NHDF, where decreasing NHDF resulted in decreased ICAM-1 in HUVEC (R2=0.45).. TNF-α caused an increase in ICAM-1 expression in NHDF under monoculture conditions (Fig. 2); this up-regulation was significantly reduced in co-culture conditions with HUVEC (1.7 vs 5.1, p,0.001). A similar trend was observed when in co-culture with HUASMC (2.7 vs 5.1, p,0.001), except the baseline expression of ICAM-1 was also increased (2.8 vs 1.1, p,0.001).. Constitutive production of ...
Mouse IL-4 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
Mouse IL-10 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
2010) (Fig. 1A). Fibroblasts were seeded at 1.5 × 105 cells/filter and HUVEC were seeded at 1.0 × 105 cells/filter to yield confluent monolayers within 24 h. After 24 h, culture media were removed and the 24-well inserts were fitted into the 12-well inserts, with 200 μl fibroblast medium added to the surface of each filter and 1.5 ml to the lower chamber. Cells were co-cultured together for 48 h, with 100 U/ml TNF alpha (R&D Systems, Abingdon, UK) in combination with 10 ng/ml IFN gamma (Peprotech Inc., London, UK) added for the second 24 h when desired. For. comparison, parallel cultures of HUVEC or fibroblasts were maintained alone on their Selleckchem AC220 original filters. To form collagen gels, ice-cold rat-tail type 1 collagen PD-166866 cell line dissolved in acetic acid (2.15 mg/ml; First Link Ltd, West Midlands, UK) was mixed with ice cold 10 × concentrated M199 in the ratio 830:170 and the pH was neutralised by addition of ice cold 1 N NaOH. For each 1 ml of gel, 160 μl FCS was ...
You know its funny that you mention tube like structures...... do they look fairly long and rectangular? I have something growing in tissue culture that looks like that. These cells started off as hemangiomas. We used collangenase to break it up, and are growing these cells in DMEM with added penicillin/streptomyacin and fetal bovine serum. Ive seen quite a few of these, one person thinks they look like myocytes....heart muscle cells in particular, they arent beating or anythying though. And if that is what they are, id be really really surprised. Most of the cells look like fibroblasts (Im thinking that what look like fibroblasts might be pericytes) with some scattered possible endothelial cells. We transfected a few dishes with a vector to induce division, but we dont see these cells in any of the tissue culture dishes containing the transformed cells. I wonder if we are looking at the same thing. Ive ordered a camera for our inverted scope, ill have to take a picture when it comes in. ...
Cell culture medium. A precision micropipette is used to transfer cell culture medium (Dulbeccos Modified Eagles medium, DMEM) to a multi-well plate. Cell culture medium (or growth medium) is commonly used in biological research to maintain and grow cells in tissue culture. Studio shot. - Stock Image C028/0709
In normal cultures, the transformation of monocytes into fibroblasts generally occurred when cells became packed together through some mechanical factors that prevented their free migration and determined their accumulation. Various modifications of the medium, the addition of dead tissue, and of trypsin or the products of trypsin digestion, failed to bring about the transformation. The inoculation af cultures of monocytes with filtered extract of Rous sarcoma frequently determined the appearance of fibroblasts. The first change undergone by the monocytes cultivated in vitro was a large increase in their size. Later, the giant monocytes became transformed into cells that did not differ essentially from those that grow from a fragment of adult connective tissue.. ...
HSV-1 infected keratinocytes release IL-1αPrimary human keratinocytes were treated with medium only, 25 μg ml-1 poly(I:C), or HSV-1 as indicated. After one ho
STAT-6 is a central mediator of IL-4-induced gene responses. It has been shown that STAT-6 is expressed in B cells and T cells upon stimulation with IL-4. Interleukin-12 (IL-12) and IL-18 are the key factors for the induction of Th1 Cells, early signals being involved in Th2 differentiation if any by IL-18 are less well characterized. We have investigatedthe mechanisms employed by IL-18 and IL-4 to control expression of STATs. Since we have shown in earlier studies that Th2 cytokines protect cells from apoptosis, we explored the possible role of the controversial cytokine IL-18 on Th2 shift and analyzed the effects of IL-18 on CD4+ T cells. Cells were cultured either with IL-18, IL-4, IL-18 + CD28 for different time points. The optimum time required for the phosphorylation of STAT-6 when stimulated with IL-18 was 72 hours. This effect of STAT-6 phosphorylation was blocked by addition of anti-IL-18 antibody in the culture. However, phosphorylation of STAT-1 and STAT-4 was seen by 24 hours. ...
Eslaminejad Mohamadreza Baghaban , Nadri Samad , Hosseini Reza Hajji Development, growth & differentiation 49(4), 351-364, 2007-05-01 医中誌Web 参考文献45件 被引用文献1件 ...
It is absolutely essential to plan and execute the experiments with utmost care. It is important to begin the planning of the microarray experiment with a proper question. The experimental model/system should be well-characterized or well-defined with an independent experimental verification. For example, if a growth factor was added which induces differentiation in 24-48 hours, but you are collecting RNA at three hours post-treatment, you should still check a parallel culture for verification that differentiation occurred at the 24-48 hours period. If possible, a quick check for a gene that is known to be affected by the treatment should be performed.. It is recommended that all experimental treatments be carried out in triplicates to compensate for biological and experimental variation. In vitro experiments using cultured cells should be conducted three different times (not three replicates performed on the same day) strictly following the same experimental procedures. Tumor specimens should ...
Prostaglandin E2 has potent effects on T cell differentiation, proliferation, survival, adhesion, migration, expression of membrane Rs and diverse synthetic responses to antigens and mitogens (Goetzl et al., 1995a). Expression of a characteristic pattern of different subtypes of EP Rs by each subset of T cells is the principal determinant of specificity of the response of those T cells to PGE2. Aspects of ligand specificity and transductional biochemical pathways have been partially defined for each of the subtypes of EP Rs expressed by T cells. The HSB.2 T cell is a "double-negative" thymocyte (Adams et al., 1970; Hara et al., 1988), that coexpresses a total of 7000 EP3, EP2 and EP4 Rs per cell with a Kdof 3.7 nM (Zeng et al., 1996a). The results of ligand binding studies (Zeng et al., 1996a) and RT-PCR analyses (fig. 1) suggested that the levels of expression of EP R mRNA and protein by unstimulated HSB.2 T cells are in the order of prevalence of EP3 Rs = EP2 Rs ,EP4 Rs, without EP1 Rs. The ...
Add 200-800 μl of the overnight culture to 350 ml of YPD medium in a 1 liter flask and grow 14-18 hours to a final concentration of 5 x 107 cells/ml (mid log phase ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
Fibroblasts synthesize the extracellular matrix of connective tissue and play an essential role in maintaining the structural integrity of most tissues. Researchers have long suspected that fibroblasts exhibit functional specialization according to their organ of origin, body site, and spatial location. In recent years, a number of approaches have revealed the existence of fibroblast subtypes in mice. Here, we discuss fibroblast heterogeneity with a focus on the mammalian dermis, which has proven an accessible and tractable system for the dissection of these relationships. We begin by considering differences in fibroblast identity according to anatomical site of origin. Subsequently, we discuss new results relating to the existence of multiple fibroblast subtypes within the mouse dermis. We consider the developmental origin of fibroblasts and how this influences heterogeneity and lineage restriction. We discuss the mechanisms by which fibroblast heterogeneity arises, including intrinsic ...
3D-Tissue Culture Reveals New Targets for Cancer Therapy 3D-Tissue Culture Reveals New Targets for Cancer Therapy (from National Cancer Institute) Over the past few years, research has shown clearly that tumor cells behave differently when allowed to grow.... Read more. ...
The NucleoSpin Multi-96 Plus Plasmid Kits are designed for purifying up to 20 µg of high-copy plasmids from up to 5 ml E. coli overnight culture. DNA is ready to use for PCR, Southern blotting, or any kind of enzymatic reaction.
A-Z Index, View All and Submit New Entry #cn-names #cn-list-body { -moz-column-count: 4; -moz-column-gap: 10px; -webkit-column-count: 4; -webkit-column-gap: 10px; column-count: 4; column-gap: 10px; } @media only screen and (max-width : 1199px), only screen and (max-device-width : 1199px){ #cn-names #cn-list-body { -moz-column-count: 3; -webkit-column-count: 3; column-count: 3; } } @media only screen and (max-width : 999px), only screen and (max-device-width : 999px){ #cn-names #cn-list-body { -moz-column-count: 2; -webkit-column-count: 2; column-count: 2; } } @media only screen and (max-width : 799px), only screen and (max-device-width : 799px){ #cn-names #cn-list-body { -moz-column-count: 2; -webkit-column-count: 2; column-count: 2; } } @media only screen and (max-width : 599px), only screen and (max-device-width : 599px){ #cn-names #cn-list-body { -moz-column-count: 1; -webkit-column-count: 1; column-count: 1; } }. ...
Thank you for your interest in spreading the word about Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
The termination of merger talks between Linde AG and Praxair Inc. over differences in corporate culture is a rare move, according to analysts and management experts.
The availability of human peripheral blood mononuclear cells (PBMC) from healthy individuals and from patients with various diseases allows many studies on normal and abnormal functions of human immune cells. Because human and murine immune biology differs in many ways, it is important that various methodologies for studying human immunology are established. The two reports highlighted in this article demonstrate the usage of human PBMCs for mechanistic and pre-clinical human immune cell studies. Read more... ...
organism. One instance that exemplifies this argument is that cells can produce protein. It isnt as simple as just making protein, and it involves
Abstract   Background & aim: Easy access, rapid recovery and high potency of monocyte cell therapy have led to special attention in cell therapy research. Monocytes are considered as sticky cells to the flask. Therefore, finding the appropriate isolation method that has the least damage to the cell and its ...
Abstract   Background & aim: Easy access, rapid recovery and high potency of monocyte cell therapy have led to special attention in cell therapy research. Monocytes are considered as sticky cells to the flask. Therefore, finding the appropriate isolation method that has the least damage to the cell and its ...
Cellular senescence, a stress induced growth arrest of somatic cells, was first documented in cell cultures over 40 years ago, however its physiological significance has only recently been demonstrated. Using novel biomarkers of cellular senescence w
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
When injected intravenously into newborn rats, eight human tissue-cultured cancer cell lines-H.Ep. #2, Detroit 6, J-111, RP 41, RP 212, Adeno Cx 1, Ovary 2, and MAC 21-grew progressively in lung and other organs of more than 50 per cent of the animals and commonly cuased death after 5-8 weeks. Two other human cell lines-the carcinoma H.Ep. #1 and the presumably normal Amnion B-grew less frequently and rarely caused death or illness.. ...
Also known as: Tnfalpha, Tnf-alpha, TNFalpha., TNFalpha+, TNFalpha When, TNFalpha ARE, TNFalpha, TNFAlpha, TNFALPHA, TNF-alpha., TNF-alpha+, TNF-alpha With, TNF-alpha The, TNF-alpha OR, TNF-alpha ARE, TNF-alpha, TNF-Alpha, TN-Falpha, T-NF-alpha, IT TNF-alpha, AT TNF-alpha ...
T-cells respond to signals from the macrophages that warn of infection. These signals are very specific, and report which type of germ is causing the infection.. For example a virus infection will cause a different signal than a bacterial infection. The signal tells the T-cell what the body is infected with and how many invaders there are.. There are millions of macrophages in the human body. If a large amount of them go to the lymph node with germs, chances are you have a pretty big infection. This is when the T-cell must call on other cells for help.. ...
Traditional methods of proteomic discovery and analysis involve breaking and rebuilding samples. Snapshot Proteomics™ by AVMBioMed provides for the high-throughput screening of whole cell lysate, allowing interactions to occur in the full presence of endogenous factors. This method of testing favors the collection of protein data from its more natural state.. ...
Every cell in the human body contains approximately the same 30,000 genes. They are located in every heart cell, every brain cell, every immune system cell, every skin cell, etc. While the majority of these genes are responsible for passing on hereditary traits to offspring, most people are unaware that there is an extremely important group of about 4,000 genes active at this moment controlling - guiding - instructing - any particular cells function. For example, in the human liver, an estimated 200 liver genes are turned-on to guide the function of each liver cell. In our immune systems white blood cells, these 200 liver genes are of course not necessary and therefore, are literally turned-off. However, on a different part of the DNA molecule there is another set of approximately 200 genes which are turned-on (active) controlling all aspects of the immune system cells. These 200 genes are controlling such things as - how each immune cell communicates with other immune cells - how each ...
With proprietary technology, cell line development capabilities, internal know-how and access to a wide range of primary cell types, we can develop industry-leading assays across a wide range of different formats to support your molecule at all stages of development.
Symbols, of particular relevance to Chemistry and Science, continued. See my uploads for similar Chemistry illustrations. Not yet collected.
Cell culture dishes / petri dishes: We focused on improved handling and stacking performance of the cell culture dishes to ensure a new level of safe
RESULTS Participants with CLS in SAT (n = 16) were similar to those without CLS in terms of ethnicity, sex, and total body fat. Individuals with CLS had greater VAT (3.7 ± 1.3 vs. 2.6 ± 1.6 L; P = 0.04), HFF (9.9 ± 7.3 vs. 5.8 ± 4.4%; P = 0.03), tumor necrosis factor-α (20.8 ± 4.8 vs. 16.2 ± 5.8 pg/mL; P = 0.01), fasting insulin (20.9 ± 10.6 vs. 9.7 ± 6.6 mU/mL; P , 0.001) and glucose (94.4 ± 9.3 vs. 86.8 ± 5.3 mg/dL; P = 0.005), and lower DI (1,559 ± 984 vs. 2,024 ± 829 ×10−4 min−1; P = 0.03). Individuals with CLS in SAT exhibited upregulation of matrix metalloproteinase-9 and monocyte antigen CD14 genes, as well as several other genes belonging to the nuclear factor-κB (NF-κB) stress pathway. ...
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture growth and scaling.
Fomin, Victor P., Blair E. Cox, and R. Ann Word. Effect of progesterone on intracellular Ca21 homeostasis in human myometrial smooth muscle cells. Am. J. Physiol. 276 (Cell Physiol. 45): C379-C385, 1999.-Although it is well known that progesterone alters uterine contractility and plays an important role in maintenance of pregnancy, the biochemical mechanisms by which progesterone alters uterine contractility in human gestation are less clear. In this investigation we sought to identify progesterone-induced adaptations in human myometrial smooth muscle cells that may alter Ca21 signaling in response to contractile agents. Cells were treated with vehicle or the progesterone analog medroxyprogesterone acetate (MPA) for 5 days, and intracellular free Ca21 concentration ([Ca]i) was quantified after treatment with oxytocin (OX) or endothelin (ET)-1. OXand ET-1-induced increases in [Ca]i were significantly attenuated in cells pretreated with MPA in a dose-dependent manner. Progesterone receptor antagonists
muscle cell in human body human coronary artery smooth muscle cells 2 photo, muscle cell in human body human coronary artery smooth muscle cells 2 image, muscle cell in human body human coronary artery smooth muscle cells 2 gallery
Nitric oxide (NO), which is known to inhibit systemic vascular smooth muscle cell proliferation, is used in the management of neonatal pulmonary hypertension. Our objectives were to determine: (1) if endogenous NO production by neonatal porcine pulmonary artery smooth muscle cells (PASMCs) varied with oxygen tension in vitro, and (2) the effect of exogenous NO and inducible NO synthase (iNOS) stimulators and inhibitors on PASMC proliferation and apoptosis. PASMCs were exposed to different conditions (varying PO2, NO donors and scavengers, iNOS stimulators and inhibitors) and proliferation, apoptosis, and cyclic guanosine 5-monophosphate (cGMP) assessed. PASMCs proliferated best between 5 and 10% O2 but cGMP levels were similar at all oxygen levels. NO donors (S-nitroso-N-acetyl-penicillamine, NOC-12, NOC-18) inhibited PASMC proliferation in a dose-dependent manner with associated cGMP increases, while NO scavengers (carboxy-PTIO), iNOS stimulators (interleukin-1β, lipopolysaccharide), and iNOS ...
TY - JOUR. T1 - Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. AU - Oh, Jin Soo. AU - Ha, Yoon. AU - An, Sung Su. AU - Khan, Momin. AU - Pennant, William A.. AU - Kim, Hyo Jin. AU - Yoon, Do Heum. AU - Lee, Minhyung. AU - Kim, Keung Nyun. PY - 2010/3/26. Y1 - 2010/3/26. N2 - Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for ...
Human muscle cell line - human body diagram at muscles. Human Muscle Cell Line encouraged for you to our website, on this period I am going to teach you with regards to Human muscle cell line.. Now, this can be a very first image, human muscle cell line, human muscle cell line atcc, human smooth muscle cell line, human skeletal muscle cell line atcc, human cardiac muscle cell line, human heart muscle cell line, human vascular smooth muscle cell line, human airway smooth muscle cell line, human skeletal muscle myoblast cell line, immortalized human skeletal muscle cell line :. ...
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. multipotent adult progenitor cells along mesodermal lineages and exhibited the enhanced expression of alkaline phosphatase and production of calcium-containing mineral debris by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells exhibited enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrixConly control groups. The potent combination of angiogenic and osteogenic properties provided by multipotent adult progenitor cells appears to create a synergistic amplification of the bone healing process. Our results indicate that multipotent adult progenitor cells have the potential to better promote tissue regeneration and healing and to be ...
TY - JOUR. T1 - Transforming growth factor-β1 mediates migration in cultured human control and exstrophy bladder smooth muscle cells. AU - Suson, Kristina D.. AU - Stec, Andrew A.. AU - Gearhart, John Phillip. AU - Shimoda, Larissa. PY - 2012/10. Y1 - 2012/10. N2 - Purpose: Transforming growth factor-β1 regulates extracellular matrix composition, and impacts function and proliferation in multiple cell types, including bladder smooth muscle cells. In this study we evaluated the response to transforming growth factor-β1 in cultured exstrophy and control bladder smooth muscle cells. Materials and Methods: Primary bladder smooth muscle cell cultures were established from patients with bladder exstrophy or vesicoureteral reflux. Smooth muscle specific α-actin and heavy chain myosin expression was determined using immunohistochemistry. Cell migration, intracellular calcium concentration and proliferation were determined after incubation for 24 to 48 hours in basal media, with or without ...
Objective: Calcific aortic valve disease is a progressive condition that shares some common pathogenic features with atherosclerosis. Transforming growth factor-β1 is a recognized mediator of atherosclerosis and is expressed in aortic valve lesions. Transforming growth factorβ1 stimulates glycosaminoglycan elongation of proteoglycans that is associated with increased lipid binding. We investigated the presence of transforming growth factor-β1 and downstream signaling intermediates in diseased human aortic valves and the effects of activated transforming growth factor-β1 receptor signaling on aortic valve interstitial cell proteoglycan synthesis and lipid binding as a possible mechanism for the initiation of the early lesion of calcific aortic valve disease. Methods and results: Diseased human aortic valve leaflets demonstrated strong immunohistochemical staining for transforming growth factor-β1 and phosphorylated Smad2/3. In primary porcine aortic valve interstitial cells, Western blots ...

3D Cell Culture : Pharmaceuticals : UK3D Cell Culture : Pharmaceuticals : UK

Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... 14:10 PANEL DISCUSSION: Will 3D Cell Culture replace animal models?. * Advantages and disadvantages of 3D Cell Culture vs ... He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell ...
more infohttps://www.smi-online.co.uk/pharmaceuticals/uk/conference/3D-Cell-Culture

A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon.  - PubMed - NCBIA method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon. - PubMed - NCBI

A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon.. Merolli A1, Mao Y1, Voronin ... Schwann cell wrapping.. Top left: a schematic drawing of a Schwann cell wrapping around an axon (black) in transverse section ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/30762018

Cell CultureCell Culture

3D Cell Culture. 3D Cell Culture. Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ... Cell Culture. Cell Culture. Create a more in vivo-like cell culture to advance your discoveries right from the start. ... 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many ... Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ...
more infohttp://www.corning.com/au/en/products/life-sciences/applications/cell-culture.html

Cell CultureCell Culture

3D Cell Culture. 3D Cell Culture. Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ... Cell Culture. Cell Culture. Create a more in vivo-like cell culture to advance your discoveries right from the start. ... 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many ... Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ...
more infohttps://www.corning.com/in/en/products/life-sciences/applications/cell-culture.html

Cell culture studyCell culture study

Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly by macrophages. Our cell ... Cell culture study * 1. Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly ... 2. Cell Culture StudiesCell Culture Studies We have Comparison of the 3 different SPIOs in the cell culture, and their effect ... Our cell culture studies showed a transient elevation in free radical of macrophages with a rapid return to the baseline. -Next ...
more infohttps://www.slideshare.net/SHAPE_Society/cell-culture-study

Cell culturesCell cultures

... ELIZ at CCIT.ARIZONA.EDU ELIZ at CCIT.ARIZONA.EDU Thu Nov 12 11:08:14 EST 1992 *Previous message: ct protein ... Networkers, I am interested in knowing how easy it would be to set up cell cultures from any transformed line of Arabidopsis. ...
more infohttp://bio.net/bionet/mm/arab-gen/1992-November/000781.html

Cell cultureCell culture

The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor ... www.concordia.ca/content/concordia/en/research/genomics/facilities-instruments/cell-culture.html ... Dedicated culture rooms. GE S110.01, SP 550.04. SP 430.01 (Whiteway).. Incubation Shakers. GE S110.05, GE S110.09, GE 230.00, ... Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated ...
more infohttp://www.concordia.ca/research/genomics/facilities-instruments/cell-culture.html

early-passage primary human cells culturedearly-passage primary human cells cultured

KudoZ) English to Spanish translation of early-passage primary human cells cultured [cell culturing - Biology (-tech,-chem, ... Medical - Biology (-tech,-chem,micro-) / cell culturing. English term or phrase: early-passage primary human cells cultured. ... Containing early-passage primary human cells cultured +/- stimulus combinations. -Concentrations covered reported clinical ... BIOMAP systems: Model complex signaling networks in primary human cell types to validate compounds and targets, identify ...
more infohttps://www.proz.com/kudoz/english_to_spanish/biology_tech_chemmicro/5384438-early_passage_primary_human_cells_cultured.html

Human Cell Culture Protocols | SpringerLinkHuman Cell Culture Protocols | SpringerLink

... a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques f ... New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. ... Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach ... Well-Differentiated Human Airway Epithelial Cell Cultures M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. ...
more infohttps://link.springer.com/book/10.1385%2F1592598617

Epithelial Cell Culture Protocols | SpringerLinkEpithelial Cell Culture Protocols | SpringerLink

In Epithelial Cell Culture Protocols, a team of well-versed experimenters and cli ... Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. ... Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium ... and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use ...
more infohttps://link.springer.com/book/10.1385/159259185X

Cell Culture FundamentalsCell Culture Fundamentals

Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). ... This webinar will introduce basic concepts of cell cultur... ... Cell culture is a fundamental technique for a variety of ... Cell Culture Fundamentals. Cell culture is a fundamental technique for a variety of applications. Cells are cultured in ... Introduction to cell culture. •Critical factors for successful cell culture. •Cell culture tips and techniques. Presenter Bio: ...
more infohttps://www.brighttalk.com/webcast/6639/365754?utm_campaign=channel-feed&utm_source=brighttalk-portal&utm_medium=web

Plant cell culturePlant cell culture

... , Date: 3 Sep 93 05:03:13 GMT , I know that plant cell and tissue culture has been around for long time. , , ... How is it that high value products such as vanilla, taxol, quinine etc , are not widely produced by plant cell culture ? ... Plant cell culture. SPLUHAR at CROP.UOGUELPH.CA SPLUHAR at CROP.UOGUELPH.CA Sun Sep 5 23:19:15 EST 1993 *Previous message: ... An example of such unstable types of cells is Alfalfa suspension cells, which frequently loose chromosomes and double others, ...
more infohttp://www.bio.net/bionet/mm/plantbio/1993-September/001703.html

Drosophila cell cultureDrosophila cell culture

... Mark D. Garfinkel garfinkl at iitmax.iit.edu Tue Feb 15 10:30:39 EST 1994 *Previous message: ... Does anybody know the origins of the Schneider cell and Kc cell lines? ,I would really appreciate any references on the ... Chapter 7 of Michael Ashburners Drosophila, A Laboratory Handbook (the big grey one) is called tissue culture. On page 118 ... he gives in-text citation of the original papers describing the origins of the cell lines, on pages 133-138 he gives the ...
more infohttp://www.bio.net/bionet/mm/dros/1994-February/000277.html

Cell Culture MediaCell Culture Media

Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and ... Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used ... Used for culture of variety of cell types under low serum conditions. Corning™ DMEM/Ham's F-12 50/50 Mix, Powder ensures ...
more infohttps://www.fishersci.com/us/en/products/I9C8K0EE/cell-culture-media.html

Cell Culture MediaCell Culture Media

Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and ... Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used ... Used for culture of variety of cell types under low serum conditions. Corning™ DMEM/Ham's F-12 50/50 Mix, Powder ensures ...
more infohttps://www.fishersci.com/us/en/products/cell-culture/cell-culture-media.html

Cell Culture LabCell Culture Lab

Tissue culture *Two separate TC labs for ML-I and ML-II tissue culture ...
more infohttps://www.utwente.nl/en/tnw/nbp/laboratories/Cell%20Culture%20Lab/

Cell Culture Flow Systems | Science | AAASCell Culture Flow Systems | Science | AAAS

Cell Culture Flow Systems. Lonza For info: 800-638-8174 www.lonza.com ... The system consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over ... Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve ... including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. Not only is the system ...
more infohttps://www.sciencemag.org/custom-publishing/new-products/cell-culture-flow-systems

Cell Culture : Pharmaceuticals : AllCell Culture : Pharmaceuticals : All

Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. The ... Day twos speaking faculty will focus on cell culture process development and cell line development including the use of high ... 11:00 Discriminating drug effects in multi-cell type 3D cell culture models. ... Day one will focus on the recent advances in 3D cell culture. Case studies will demonstrate how this method is providing highly ...
more infohttp://www.smi-online.co.uk/pharmaceuticals/archive/7-2013/conference/cell-culture?o=login&dl=br&p1=4068

PharmaVision, partnered with | Cell CulturePharmaVision, partnered with | Cell Culture

Europes leading Cell Culture and Downstream Processing conference for upstream and downstream manufacture ... Cell Culture & Downstream World Congress 2017. 21 - 22 February. Sofitel Munich Bayerpost, Munich ...
more infohttp://www.terrapinn.com/conference/cell-culture/partner-pharmavision.stm

Cell culture medium - Genetix LimitedCell culture medium - Genetix Limited

... in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability ... We describe a method of growing an animal cell in a culture medium, ... Specific examples of cells include CHO cells, HeLa cells, HEK cells, HEK 293 cells, COS1 cells, embryonic stem cells, African ... Animal Cell The improved cell culture medium is suitable for the culture of any cell or colony or cell line preferably of ...
more infohttp://www.freepatentsonline.com/y2007/0212778.html

Cell Culture | SartoriusCell Culture | Sartorius

... the filtration of cell culture media, biological and other aqueous solutions with ready-to-use vacuum filters and pressure ... filters; cell cultivation via incubators, incubation and benchtop shakers and cell harvest via homogenisers and centrifuges ... Cell culture products for measuring the pH value and redox voltages, ... Cell culture products for measuring the pH value and redox voltages, the filtration of cell culture media, biological and other ...
more infohttps://www.sartorius.com/shop/us/en/usd/applications-laboratory-cell-culture/c/TAXONOMY00127

Cell CultureCell Culture

... & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Cell Culture Flasks. Enhance cell attachment, growth and differentiation with Thermo Scientific™ Nunc™ Cell Culture Treated ... Thermo Scientific™ BioLite Cell Culture Treated Dishes. Perform general cell culture procedures more economically with Thermo ... Corning™ U-Shaped Cell Culture Flasks. Designed to improve usability while maintaining the same environment for cell growth as ...
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Cell culture questions | Physics ForumsCell culture questions | Physics Forums

How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth? #If medium with ... I wash my cells with some PBS and then do the digestion in some PBS with trypsin. Just dont leave the PBS on the cells too ... How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth?. #If medium with ... That means less oxygen (which is a stress factor to the cells) and more CO2, cells wont grow without it. CO2 is also part of ...
more infohttps://www.physicsforums.com/threads/cell-culture-questions.67890/

3D Cell Culture : Pharmaceuticals : UK3D Cell Culture : Pharmaceuticals : UK

Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... 14:10 PANEL DISCUSSION: Will 3D Cell Culture replace animal models?. * Advantages and disadvantages of 3D Cell Culture vs ... He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell ...
more infohttp://smi-online.co.uk/pharmaceuticals/uk/conference/3D-Cell-Culture

Cell CultureCell Culture

These disposable, space saving containers are specifically designed for optimal plant cell culture applications. ... These disposable, space saving containers are specifically designed for optimal plant cell culture applications. ... Sterilized Cover for PLANTCON ™ Plant Tissue Culture Container. The PlantCon™ container consists of a cover and a bottom, and ... Sterilized Base for PLANTCON ™ Plant Tissue Culture Container. The PlantCon™ container consists of a cover and a bottom, and ...
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  • Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, and discover novel 3D cell culture systems, organ-on-chip, 3D imaging of organoids and other technologies in development. (smi-online.co.uk)
  • Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano- and submicron-scale polystyrene and polycarbonate fibrous mats (now known as scaffolds) specifically intended for use as in vitro cell substrates. (wikipedia.org)
  • Oxygen-sensing transcription systems, such as hypoxia-inducible factors, play an important role in regulating the differentiation of stem cells ( 3 ), as does the distribution of diffusing growth factors. (sciencemag.org)
  • However, the complexity, variability, and ill-defined nature of these interactions make it difficult to understand the proliferation, differentiation, and migration of cells embedded within these natural gels. (sciencemag.org)
  • New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. (springer.com)
  • The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. (springer.com)
  • Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. (springer.com)
  • Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (springer.com)
  • Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (springer.com)
  • cell cultivation via incubators, incubation and benchtop shakers and cell harvest via homogenisers and centrifuges with varoius volumes as well as PCR-based tests for mycoplasma contamination control. (sartorius.com)
  • One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
  • Click here to register for free and to view the entire webinar: http://bit.ly/kosi_136 Since 2008 Kaiser Optical has worked closely with industry leading bioprocessing companies to develop a Raman solution enabled to deliver in situ, real-time measurement to a host of bioprocessing applications including but not limited to cell culture, fermentation, and purification. (environmental-expert.com)
  • Corning™ U-Shaped Cell Culture Flasks have an ergonomic shape that reduces the number of corners, improves cell scraping and allows the use of a larger pipet. (fishersci.ca)
  • Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap offer a convenient solution to prepare laboratory samples. (fishersci.ca)
  • We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. (freepatentsonline.com)
  • 18. A method according to claim 1, in which cell growth or cell viability is enhanced by at least 50% compared to growth in a cell culture medium without an elevated concentration of thymidine family member. (freepatentsonline.com)
  • 19. A method according to claim 1, in which apoptosis of the animal cell in the cell culture medium is reduced to enhance cell viability. (freepatentsonline.com)
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • Physiological relevance is a key parameter to improve predictivity of cell-based assays in drug discovery and toxicity testing for compound. (dechema.de)
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • Case studies will demonstrate how this method is providing highly physiologically accurate preclinical data and current approaches for culture in relevant diseases. (smi-online.co.uk)
  • Researchers are now bridging the gap between natural and synthetic gels by combining well-characterized synthetic materials with biomimetic cues to support physiologically relevant cell-gel interactions (see the figure). (sciencemag.org)
  • Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (springer.com)
  • A team of scientists led by Prof. Antonella Consiglio from the IDIBELL and the University of Barcelona, and Prof. Angel Raya from the Center of Regenerative Medicine of Barcelona have discovered that defective versions of human brain cells called astrocytes are linked to the buildup of a toxic protein that is one the hallmarks of Parkinson's disease. (news-medical.net)
  • The spread of pathogens like the human immunodeficiency virus (HIV) is often studied in a test tube, i.e. in two-dimensional cell cultures, even though it hardly reflects the much more complex conditions in the human body. (news-medical.net)
  • Article by Leonard Hayflick and Robert M Chanock on the general properties of the genus Mycoplasma, the human species, their role in human disease and how they often contaminate cell cultures. (dmoztools.net)
  • Tina Stoschek and Marcus Gerlach discuss maximizing mAb generation using lab-scale clarification of cell cultures expressing recombinant antibodies. (news-medical.net)
  • Mycoplasma is a genus of bacteria the cells of which lack a cell wall and are unaffected by many common antibiotics. (dmoztools.net)
  • Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. (corning.com)
  • Conference on 3D cell cultures as advanced model systems for understanding of diseases, compound testing, successful translation from models to clinical and industrial solutions, and on enabling technologies. (dechema.de)
  • The continually rising numbers of compound failures and increasing costs of drug and substance development are fostering the use of biologically more complex cell models from target identification to safety testing in substance development. (dechema.de)
  • Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
  • Our 3-day event, formed of two half day post-conference workshops and a two-day conference will provide useful insights into the latest developments within cell culture. (smi-online.co.uk)
  • A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon. (nih.gov)
  • To uncouple these effects and focus on the direct response of Schwann cells, we developed an in vitro model where an electroconductive carbon fiber acts as an artificial axon. (nih.gov)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Regardless of the nature of the hydrogel, challenges must be overcome related to the general approach of three-dimensional cell culture. (sciencemag.org)
  • The cover can be snapped on tight, for long-term culture, or loosely fitted during acclimation to external conditions prior to transplantation. (mpbio.com)
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (wikipedia.org)
  • Culture conditions vary widely for each cell type, and variation of conditions for a particular cell type can result in different phenotypes. (wikipedia.org)
  • To maximize cell yield, choose from our comprehensive line of standard and custom culture media, including basal salt solutions, antibiotics, sera, and specialty media. (corning.com)
  • The lipid bilayer membrane of the Schwann cell (yellow) expands and wraps around the axon keeping a very tiny cytoplasmic space (green). (nih.gov)
  • Moreover, cells grown in 3D exhibit different gene expression than those grown in 2D. (wikipedia.org)
  • Often following diagnosis and treatment for cancer and other diseases, patients' organs and cells can remain healed but damaged from the medical condition. (news-medical.net)
  • Conference on 3D cell cultures as advanced model systems for understanding of diseases, compound testing and screening, and on enabling technologies. (dechema.de)
  • With the advent of automated cell counters 12-15 years ago, disposable slides became widespread. (news-medical.net)
  • AXT is pleased to announce it has further enhanced its cellular research product portfolio through the addition of stem cell solutions from StemBioSys. (news-medical.net)
  • First, even in two-dimensional culture, heterogeneities exist in the cellular microenvironment, and these will only be further exaggerated in three-dimensional gels. (sciencemag.org)