The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)

The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen.  (+info)

In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)

Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (6/155046)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

TY - JOUR. T1 - Lipopolysaccharide extends the lifespan of mouse primary-cultured microglia. AU - Kaneko, Yoko S.. AU - Nakashima, Akira. AU - Mori, Keiji. AU - Nagatsu, Toshiharu. AU - Nagatsu, Ikuko. AU - Ota, Akira. PY - 2009/7/7. Y1 - 2009/7/7. N2 - Microglial activation has been implicated in the recognition and phagocytic removal of degenerating neurons; however, this process must be tightly regulated in the central nervous system, because prolonged activation could damage normal neurons. We report that mouse primary-cultured microglia, which are destined to die within a few days under ordinary culture conditions, can live for more than 1 month when kept activated by lipopolysaccharide (LPS) treatment. Primary-cultured microglia treated with sublethal doses of LPS remained viable, without any measurable increase in apoptotic or necrotic cell death. LPS-treated microglia had an arborescent shape, with enlarged somata and thickened cell bodies. Although the amount of intracellular ATP in ...
Langford test 1 (from quizlet) - infectious diseases 4180 with at muscles. Human Muscle Cell Can Perform Quizlet pleasant in order to the web site, within this moment I will explain to you with regards to Human muscle cell can perform quizlet.. And from now on, this can be the very first picture, human muscle cells can perform quizlet :. ...
The present study was undertaken to elucidate different requirements for CD2-mediated activation of naive (CD45RO-) and memory (CD45RO+) CD4+ T cells. A mitogenic combination of anti-CD2 (anti-T11(2) and anti-T11(3] mAbs could effectively induce the proliferation of memory CD4+ T cells even in the absence of monocytes. In marked contrast, naive CD4+ T cells did not disclose any proliferative responses to anti-CD2 mAbs, when monocytes were absent in culture. This differential responsiveness of naive and memory CD4+ T cells appeared to be related largely to a difference in IL-6-producing ability between both populations. IL-6 among monocyte-derived cytokines could correct unresponsiveness of naive CD4+ T cells to anti-CD2 stimulation. Unlike naive CD4+ T cells, memory CD4+ T cells produced IL-6 by themselves, with its mRNA being expressed on anti-CD2 stimulation. Anti-IL-6R mAb significantly inhibited proliferation of memory CD4+ T cells seen in the anti-CD2-stimulated cultures without monocytes, ...
Differential responsiveness of naive and activated T cells to TL1A that affect Th17 generation, maintenance and proliferation [Abstract]. Immunology 131 (s1) , p. 102. 10.1111/j.1365-2567.2010.03390.x ...
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Microbiology culture preparation. Person using a bunsen flame to sterilise the top of a jar containing a bacterial culture. - Stock Image C024/5723
We offer 4 types of normal human primary cells in the adipose cell system including endothelial cells, preadipocytes and mesenchymal stem cells.
Author: Sözen, Cécile et al.; Genre: Journal Article; Published in Print: 2020-06; Title: Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics
Growth Requirements. The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. To obtain best growth, addition of a small amount of blood serum is usually necessary, and several antibiotics, like penicillin and streptomycin are added to prevent bacterial contamination.. Temperature varies on the type of host cell. Most mammalian cells are maintained at 37oC for optimal growth, while cells derived from cold- blooded animals tolerate a wider temperature range (i.e. 15oC to 26oC). Actively growing cells of log phage should be used which divide rapidly during culture.. Process to obtain primary cell culture. Primary cell cultures are prepared from fresh tissues. Pieces of tissues from the organ are removed aseptically; which are ...
Human Prostate Microvascular Endothelial Cell Pellet ...
TY - JOUR. T1 - Transforming growth factor-β in GI neoplasia, wound healing and immune response. AU - Pignatelli, Massimo. AU - Gilligan, Christopher J.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - The last decade has been marked by tremendous advances in the biochemical and functional characterization of TGF-βs and their receptors in normal and transformed cells. TGF-βs have been shown to modulate proliferation, differentiation and motility of different cell types in a number of in vitro model systems and in some cases with some intriguing results. It is obvious that there is no simple pattern that explains the TGF-βs biological activity in vitro and their effects on cell behaviour need to be assessed in the context of an appropriate physiological cellular environment. Cell-cell and cell-matrix interactions, the differentiating status of the cell together with the functional activity of other soluble growth factors can influence how TGF-βs modulate cell behaviour. However, the overwhelming interest ...
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. 
Human Esophageal Fibroblast Cell Pellet ...
In 1994, Cell Applications, Inc. recognized the demand for primary cells and set out to galvanize this paradigm shift from cell lines to primary cells. With years of experience handling numerous notoriously finicky primary cell types, we have honed the craft of isolation, purification, subculture, and growth of primary cells. Our company is built on high purity, strictly quality controlled and characterized primary cells, including over 80 different human and animal primary cell types, as well as cell growth media and cell culture reagents, cell marker and signaling antibodies. These comprise a continuously growing cell culture toolbox. ...
CellSeed, in Japan, is developing a series of cell therapies based on its regenerated human cell culture technology (UpCell®) for the potential treatment of
PromoCells facility remains operational and we continue to support researchers worldwide. We are proud of making a contribution to the fight against SARS-CoV-2 with our products. We are here to help - contact us. Dismiss. ...
Given the fact that IL-10 is especially produced by immune cells, we investigated whether the same is true for the other members of the IL-10 family, IL-19, IL-20, IL-22, mda-7, and AK155, and, if so, to what extent. Monocytes, NK cells, B cells, and T cells from the blood of different healthy donors were investigated for their possible constitutive, as well as activation-induced, expression of these molecules. For this purpose, cells were cultured in a kinetic approach in the absence and presence of the typical cell-specific stimuli LPS (monocytes), IL-2/IL-12 (NK cells), fixed S. aureus (B cells), and anti-CD3 mAb coated on the culture vessel (T cells), respectively, for 2, 6, and 18 h. The activation state of control and stimulated cells was verified by the absence and presence of high TNF-α production in the monocyte culture and elevated expression of different activation markers, e.g., CD69, on the lymphocyte populations (Fig. 1⇑A). Gene expression of the IL-10 family members was ...
Ongoing advances in quantitative molecular- and cellular-biology highlight the need for correspondingly quantitative methods in tissue-biology, in which the presence and activity of specific cell-subpopulations can be assessed in situ. However, many experimental techniques disturb the natural tissue balance, making it difficult to draw realistic conclusions concerning in situ cell behaviour. In this study, we present a widely applicable and minimally invasive method which combines fluorescence cell labelling, retrospective image analysis and mathematical data processing to detect the presence and activity of cell subpopulations, using adhesion patterns in STRO-1 immunoselected human mesenchymal populations and the homogeneous osteoblast-like MG63 continuous cell line as an illustration.. Adhesion is considered on tissue culture plastic and fibronectin surfaces, using cell area as a readily obtainable and individual cell specific measure of spreading. The underlying statistical distributions of ...
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
We are the worlds largest primary cell provider, with a variety of normal human and animal primary cells frozen at early passage, cell culture media and reagents, growth factors, cell-derived molecular biology products, cell-based assay kits, stem cell products and gene analysis tools.
Click HERE to find out ⭐ SeedInvest Lists 2nd Potential Regulation A+ Offer with Snapwire. $5 Million in Indicated Interest in Just 2 Days. | Crowdfund Insider: Global Fintech News, including Crowdfunding, Blockchain and more.
The Knowledge Gateway is our open source access to resources. Here is where we share our accumulated resources with this request: Please use them freely yourself, referencing their source, and let us know how you used them and any improvements you made. ...
The PromoCell immuno-oncology portfolio contains characterised human primary cells, including vascular and microvascular endothelial cells, mononuclear cells, macrophages, monocytes and blood progenitor cells - plus optimized cell culture growth medium for each cell type.
Our ethically sourced human primary cell portfolio includes fresh and cryopreserved products from peripheral blood and cord blood.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
8. This allows further bacterial multi- plication topama subsequent production of an increased amount of inflammatory mediators via the complement system and other pathways.
A self-contained mini-greenhouse useful for propagating cuttings, germinating seeds, and acclimating tissue-cultured plants intended for transfer to soil. Includes plastic tray …
MaxATP has what you need to achieve optimum physical performance during work, play, and the demands of the day. Natural performance comes from your bodys natural energy source, cellular production of Adenosine triphosphate (ATP).
Study Flashcards On PA 2008- Patho ch. 2 at Quickly memorize the terms, phrases and much more. makes it easy to get the grade you want!
Study Flashcards On patho at Quickly memorize the terms, phrases and much more. makes it easy to get the grade you want!
The human system, critical infrastructure, environment, human society, human etc. are represented by system of systems, i.e. they consist...
Cell culture media and reagents for the stimulation, expansion, and differentiation of human and mouse primary cells, including immune cells, stem cells, and neural cells. - Deutschland
هدف: سلول‌های بنیادی پرتوان مشتق شده از بافت بیضه، یک منبع بسیار غنی و جدیدی برای سلول درمانی در پزشکی بازساختی هستند. مطالعات اخیر نشانگر آن است که سلول‌های بنیادی اسپرماتوگونی در شرایط آزمایشگاهی می‌توانند به سلول‌های بنیادی شبه جنینی تغییر یابند.استفاده از امواج فراصوت با شدت پایین، آثار مثبتی روی رشد و تمایز سلول‌ها دارد. هدف از این مطالعه تأثیر امواج فراصوت روی کلونی‌زایی سلول‌های بنیادی شبه جنینی است. مواد و روش‌ها: ابتدا، سلول‌های بنیادی اسپرماتوگونی از بیضه نوزاد موش جدا شدند. بعد از کشت دادن این سلول‌ها در محیط DMEM/F12، حاوی 15 درصد سرم جنین
Духи ★★★ «EX NIHILO» ★★★ (Экс Нихило) ☎ (044) 38-38-209 в Украине! Только оригинальная парфюмерия EX NIHILO! Суперцены!
The purpose of the present investigation was to explore the effects of well-defined flow conditions on the activity of tissue factor (TF) expressed on the surface of cultured rat vascular smooth muscle cells. Cells were cultured to confluence on Permanox brand slides and stimulated to express TF by a 90 min incubation with fresh growth medium containing 10 percent calf serum. The stimulated cells were then placed in a parallel plate flow chamber and perfused with Hanks Balanced Salt Solution containing factor VIIa, factor X (FX), and calcium. The chamber effluent was collected and assayed for factor Xa (FXa) and the steady-state flux of FXa was calculated. The flux values were 68.73, 94.81, 139.75, 138.19, 316.82, and 592.92 fmole/min/cm2 at wall shear rates of 10, 20, 40, 80, 320, and 1280 s−1 respectively. The FXa flux depended on the wall shear rate to a greater degree than predicted by classical mass transport theory. The flux at each shear rate was three to five times less than that ...
TY - JOUR. T1 - Modulation of collagen synthesis by tumor necrosis factor alpha in cultured vascular smooth muscle cells. AU - Hiraga, Syouichi. AU - Kaji, Toshiyuki. AU - Ueda, Yoshimichi. AU - Zisaki, Fumiko. AU - Iwata, Kazushi. AU - Koizumi, Fumitomo. AU - Okada, Yasunori. AU - Katsuda, Shogo. AU - Nakanishi, Isao. PY - 1999/12/10. Y1 - 1999/12/10. N2 - Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. The synthesis of collagenase-digestible proteins (CDP) and noncollagenous proteins (NCP) was evaluated by the [3H]proline incorporation. It was shown that TNF-α markedly suppresses the incorporation of [3H]proline into both CDP and NCP in confluent cultures of SMCs but not in sparse cultures of the cells. Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. In confluent SMCs, the synthesis of CDP was more ...
I am planning to set up a co-culture system for human endothelial cells and human VASCULAR smooth muscle cells. I would be really interested to find out about methods of extraction and primary culture of human vascular smooth muscle cells, if anyone can help and advise, please mail me! Thanks Pippa Deex ...
Vascular smooth muscle (VSM) cell proliferation contributes to the pathogenesis of atherosclerosis, restenosis after angioplasty and vein graft disease. The regulation of genes involved in VSM cell proliferation, particularly by naturally occurring inhibitors, is therefore of some importance. We have investigated the role of the c-myc proto-oncogene in growth arrest of exponentially proliferating rat VSM cells, following mitogen withdrawal, treatment with heparin (50 micrograms/ml), interferon-gamma (IFN-gamma) (100 i.u./ml), or the cyclic nucleotide analogues, 8-bromo-adenosine-3′5′-cyclic monophosphate (8-Br-cAMP; 0.1 mM) and 8-bromoguanosine-3′5′-cyclic monophosphate (8-Br-cGMP; 0.1 mM). Growth arrest was accompanied by down-regulation of c-Myc protein and mRNA following treatment with all inhibitors. Serum withdrawal or IFN-gamma treatment suppressed c-myc expression by more than 50% within 2 h, and this occurred throughout the cell cycle. Platelet-derived growth factor, epidermal ...
The concept of arterial SMC heterogeneity has gained wide acceptance in the last years.1 2 33 The distinct phenotypes of arterial SMCs have been mainly identified in vitro,4 5 6 7 8 9 10 11 12 13 14 15 16 suggesting that specific features of SMC populations arise and are maintained in the particular environment of cell culture. Hence, it was of interest to investigate whether in vitro SMC phenotypes are preserved when SMCs are placed back in an in vivo environment. For this purpose, we have implanted 2 SMC populations exhibiting distinct levels of differentiation in vitro into the rat carotid artery submitted to endothelial injury.24 25 The implanted SMCs were marked with PKH-26, a lipophilic cell membrane linker that is halved with each cell division but is not lost from the cell membrane.34 Our results show that the 2 implanted populations essentially retain for 20 days in vivo the phenotype that they specifically exhibited in vitro.. Spindle-shaped and epithelioid rat SMC populations have ...
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbeccos medium supplemented wi
Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
Jochen Meier Ivonne Strmel Radu Iosub Sonja Schmidt and Rosemarie Grantyn Developmental Physiology Johannes Mller Institute Humboldt University Medical School (Charit) Berlin Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance,Effectene,Transfection,Reagent,provides,efficient,gene,delivery,,,,,,,,,,,,to,primary,neuronal,cell,cultures,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Jacobs, B B., In vivo assay of function of mouse ovaries following culture in hormone enriched medium. (1963). Subject Strain Bibliography 1963. 546 ...
Full Text - Hydrogen peroxide (H2O2) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H2O2-treated human osteoblasts were tested. We show that H2O2 significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H2O2-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H2O2-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H2O2 induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124
Many studies have shown that TRAIL is a potent apoptosis inducer in malignant cells, whereas its role in normal cell physiology is much less well understood.1 We have demonstrated here that both aortic endothelial cells and HUVECs exhibit a similar pattern of surface TRAIL-R expression. Moreover, in vascular endothelial cells, TRAIL stimulates the phosphorylation of the serine/threonine kinase Akt in a manner dependent on PI3K activation.14 The ability of TRAIL to activate the antiapoptotic PI3K/Akt pathway in endothelial cells is a completely new and unexpected finding, also in consideration of the large number of studies underlining the proapoptotic activity of TRAIL, at least in malignant cells.1,4,5 It should be underlined that the PI3K/Akt pathway is of central importance in endothelial cell biology, conferring survival to endothelial cells in response to angiogenic cytokine stimulation, fluid shear stress, and matrix attachment signals. This pathway is essential also for endothelial cell ...
TY - JOUR. T1 - Activation of caspase-8 and Erk-1/2 in domes regulates cell death induced by confluence in MDCK cells. AU - Chang, Yung Heng. AU - Lin, Hsi Hui. AU - Wang, Yang Kao. AU - Chiu, Wen Tai. AU - Su, Hsiao Wen. AU - Tang, Ming Jer. PY - 2007/4/1. Y1 - 2007/4/1. N2 - Under normal culture conditions, cells adhere to culture dish, spread out, proliferate, and finally cover all areas and reach confluence. During the confluent stage, cell proliferation ceases and differentiation is enhanced. Meanwhile, cell death also appears as the monolayer confluence proceeds. To delineate the mechanism of cell death induced by the confluent process, we employed Madin-Darby canine kidney (MDCK) cells. When approaching confluence, MDCK cells exhibited increase the levels of caspase-2 and enhanced the activity of caspase-8. Using various caspase inhibitors to block apoptosis, we found that only z-VAD-fmk and z-IETD-fmk can inhibit confluent cell death, indicating that confluent cell death is mediated by ...
In the present article, we compared the antiangiogenic activity of trans- and cis-resveratrol on endothelial cells of different origin activated by endogenous or exogenous stimuli. The results indicate that trans-resveratrol affects endothelial cell proliferation and various cell proliferation-independent aspects of the angiogenesis process, including endothelial cell sprout formation, collagen gel invasion, and morphogenesis. Thus, trans-resveratrol acts as an angiostatic molecule on multiple targets of the angiogenic process. In all the assays, the cis stereoisomer exerted only a limited effect, if any. In keeping with the in vitro observations, trans-resveratrol was more effective than the cis isomer in inhibiting angiogenesis and tumor growth in vivo.. The molecular bases of the antiangiogenic activity of resveratrol are poorly understood (16). trans-Resveratrol is endowed with a significant antioxidant and anti-inflammatory activity (8, 9). However, no direct relationship exists between the ...
TY - JOUR. T1 - Recombinant murine and human IL 1α bind to human endothelial cells with an equal affinity, but have an unequal ability to induce endothelial cell adherence of lymphocytes. AU - Thieme, T. R.. AU - Hefeneider, S. H.. AU - Wagner, C. R.. AU - Burger, D. R.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - Consistent with the reports of others, we have demonstrated that human peripheral blood lymphocytes adhere to cultured human umbilical vein-derived endothelial cells (EC) in vitro. In our studies adherence was increased twofold to threefold by a 6-hr preincubation of the EC with IL 1. Recombinant human IL 1α induced a maximal adherence response at less than 1 U per 2 x 104 EC. In contrast, recombinant murine IL 1α was found to be 250- to 1250-fold less active in the adherence assay, based on units of IL 1 activity defined by the murine thymocyte proliferation assay. Moreover, when EC were preincubated with excess murine IL 1, no inhibition of the adherence-inducing effect of human IL 1 was ...
The effects of interferons (IFNs) on lymphocyte adhesion to cultured human vascular endothelial cells (EC) were investigated using an in vitro assay. Endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (CM) from 12-O-tetra decanoylphorbol 13-acetate-concanavalin A (TPA-Con A) stimulated human peripheral blood lymphocytes (PBL), or with recombinant (r) gamma interferon (IFN-gamma) or r alpha interferon (IFN-alpha), and then were incubated with freshly isolated PBL. Natural IFN-gamma in the TPA-Con A CM and rIFN-gamma (12.5-500 U/ml) induced major histocompatibility complex-class II antigens (HLA-DR, HLA-DP, and HLA-DQ) and significant lymphocyte adhesion to the EC, whereas rIFN-alpha did not. The lymphocyte adhesion to the EC and the expression of DR antigens on the EC were well correlated in terms of both kinetics and the dose-response pattern of rIFN-gamma. When EC expressing I region associated (Ia) antigen were preincubated with ...
The fully sequenced pCERI is transformed into E.coli BL21 and E.coli Nissle 1917 that are used for the characterization of our regulatory system. The expression efficiency of the genes of interest is characterized by a fluorescence-based assay (Results) Overnight cultures of both recombinant strains are inoculated with single colonies. The overnight cultures are diluted with LB-Amp to an OD of 0.02. 200 μ of the dilutions are pipetted in microtiter plates and incubated at 37 °C in the plate reader. Every 15 minutes mRFP fluorescence (Ex. 584 nm, Em. 607 nm), CFP fluorescence (Ex. 439 nm, Em. 476) and OD600 is measured over a period of 13 h. The measured RFU (relative fluorescence unit) is either blotted against time or against OD600 to evaluate the expression behavior of pCERI. Additionally several samples are either induced with 0.1 -50 nM of 3OC6-HSL and 0.1-100 nM of C8-HSL to explore putative advantages of higher HSL-synthesis, that might be possible by positioning each HSL-synthase, ...
This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for ...
Choice of AMP 100 versus AMP 50 - posted in Molecular Cloning: Ok, i know the main difference is the amp concentration. But i wonder if it matters that i use amp 50 for overnight culture versus amp 100 for overnight culture.
I am studying the differentiation of leukemic cells along the monocytic lineage using calcitriol as differentiative agent and others vitamins as possible stimulating compounds on differentiation. In my work I have used HL-60 cells as in vitro culture cells but now I have some problems because these cells have changed their response and develop expontaneous differentiation in normal culture conditions. Now, I am thinking on NB4 cells as a better cell line to study differentiation. I have ask some information to cell banks as ATCC and ECA but they dont have this cell line. Could anybody tell me if anyone can send me this cell line?, I have very gratefull if anyone could send me HL-60 cells too. Waiting positive responses and acknowledging in advance. Guillermo Lopez-Lluch Departamento de Biología Celular Facultad de Ciencias Universidad de Córdoba Avda. S. Alberto Magno S/N E-14007 Córdoba, Spain. TFNO: Int + 34-57-218634 FAX: Int + 34-57-218634 e-mail: bc2biced at ...
TY - JOUR. T1 - Cell interactions in nerve and muscle cell cultures.. AU - Christian, C. N.. AU - Bergey, G. K.. AU - Daniels, M. P.. AU - Nelson, P. G.. PY - 1980/12/1. Y1 - 1980/12/1. N2 - The neurotransmitter synthesized by a given class of neurones is subject to modification and, indeed, a qualitative switchover in transmitter biochemistry recently has been demonstrated (Furshpan, POtter & Landis, 1980; Walicke, Campenot & Patterson, 1977). In conjunction with the specification of transmitter biosynthesis that becomes established in a given neurone, a complementary specification of appropriate receptor production is required in any cell functionally post-synaptic to that neurone. An additional requirement of peculiar force in the nervous system has to do with the spatial organization of the receptors in the surface membrane of the post-synaptic cell once the receptors are synthesized. Inappropriately distributed receptors are useless receptors. The perfect registration of a variety of types ...
I have provided this website and/or the information for educational and community purposes only. Nothing contained in here is intended to be instructional for medical diagnosis or treatment. Although I make a conscientious effort to provide high quality information, I disclaim any implied guarantee about the accuracy, completeness, timeliness or relevance of any information. For any questions concerning medical needs, visit or call your CF Center or health care professional. This web site is meant for the community for support and information. I do not give medical instruction or treatment. All health information found on this blog including search engine resources, may not be right for your medical conditions and needs. Always consult your doctor, healthcare specialist/practitioner or medical center when determining which course of health care regime is best for your individual care.. ...
TY - JOUR. T1 - Studies on the mechanism of hrmonal stimulation of zinc uptake in human cell cultures. T2 - hormone-cell interactions and characteristics of zinc accumulation.. AU - Cox, R. P.. AU - Ruckenstein, A.. PY - 1971/2. Y1 - 1971/2. UR - UR - M3 - Article. C2 - 4322943. AN - SCOPUS:0015012917. VL - 77. SP - 71. EP - 81. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 1. ER - ...
One group of projects in the lab concerns co-receptors, cell-surface molecules that regulate the interaction between soluble growth factors and their cell-surface receptors. We have identified a novel co-receptor for BMPs (bone morphogenetic proteins) and are studying the roles of Heparan Sulfate Proteoglycans (HSPGs) as co-receptors for a variety of growth factors, including those that drive tumor development and pattern the developing nervous system. We have used knockout technology to generate mice that are mutant for particular HSPGs, and are also working with mice that are mutant for some of the biosynthetic enzymes required to make HSPGs. We are also using modeling approaches to understand how HSPGs influences the kinetic parameters of growth factor-receptor interaction ...
Were stained with rat endothelial cell antigen. + pixels were counted in glomeruli and tubular fields working with ImageJ Application . To be able to evaluate
How would the cells respond to Aβ? Mancuso injected 5 μL of 10 μM synthetic oligomers into the mouse brain ventricles eight to 10 weeks after transplanting the human microglia. In reaction to this insult, endogenous mouse microglia shifted their transcriptomes sequentially from homeostatic to cytokine responsive, to activated, with the latter partially overlapping with disease-associated microglial (DAM) signatures described previously (Jun 2017 news). Human microglia in the mice underwent a transcriptional transformation as well, but analysis of more than 10,000 orthologous genes indicated poor correlation between human and mouse microglial responses. Of 207 differentially activated genes, 112 were up in human but not mouse microglia; they included GWAS hits BIN1 and PICALM. We saw that human microglia responded very differently to Aβ oligomers. This emphasizes the need to look specifically at human cells in the context of AD, said De Strooper.. Blurton-Jones and colleagues took a ... Amruta Phatak, Mohammad Athar, James A. Crowell, David Leffel, Brittney-Shea Herbert, Allen E. Bale, Levy Kopelovich
Informationen über Tissue Culture Plates, adherent, treated, 24 well, working vol: 1-3.5 ml,Growth area 1.9 cm2 (NEF-TPP24-1X200NO)
Gentaur molecular products has all kinds of products like :Cell_Technology , Cell Technology \ aCella - TOX 500 Tests (with 5 Lumi Plates + 5 Tissue Culture Plates) \ CLATOX100-3P for more molecular products just contact us
Recent evidence supports a strong role for immune suppression in facilitating cancer progression. Transforming growth factor beta (TGFß) regulates a diverse array of biological events, including neoangiogenesis, stromal formation, and immune responses. Both TGFß and a small molecule inhibitor of TGFßR-1, LY364947, were effective regulators of these processes in vitro. Using quantitative RT-PCR and fluorescence imaging, TGFß increased collagen production by pancreatic stellate cells while addition of LY364947 suppressed production below basal levels. Endothelial cell proliferation, measured as a function of endothelial tube formation in Geltrex® matrix, was similarly increased by TGFß and reduced to basal levels by LY364947. To explore the in vivo effects of these molecules, LY364947 was encapsulated in liposomes to encourage tumor accumulation and to avoid administration of DMSO vehicle. Liposomes were prepared with a molar ratio of 6:3:1 1,2-dipalmitoyl-sn-glycero-3-phosphocholine ...
Simple muscle cell cultures are utilized frequently to investigate the mobile mechanisms of contraction. in Personal computer and RC vs .. Me personally and FC (all < 0.05). Appearance of Gi3, serine/threonine proteins phosphatase-1 -catalytic subunit, and Rho kinase 1 improved in Personal computer and RC vs .. Me personally and FC (all < 0.05). Cell tradition and resuspension downregulated appearance of -actin and calponin, but not really myosin weighty string. The online impact of these molecular adjustments was reductions of cell reactivity to ACh in RC vs .. FC. Overexpression of CPI-17 in Personal computer partly reversed the reductions of contractility in resuspended cells. Methylation-specific PCR demonstrated improved methylation of the gene marketer in Computer vs .. Me personally (< 0.05). We agreed that even muscles cells preserve their contractile phenotype in lifestyle. Nevertheless, reactivity to ACh diminishes because of changed reflection of particular cell-signaling protein ...
Conventional biology expects the process of mammalian cell division, mitosis, to occur by the equal partition of a mother cell into two daughter cells. Bioengineers at UCLA Engineering have developed a platform that mechanically ...
A growing body of evidence suggests that transiently activated NF-κB in noninflammatory states and persistently activated NF-κB during inflammation play different pathophysiological roles in vivo. During the initial phase of inflammation, proinflammatory cytokines and mediators induce prolonged NF-κB activation in various inflammatory cells and endothelium. The activated NF-κB further upregulates the expression of several proinflammatory molecules, such as endothelial cell adhesion molecules and macrophage inflammatory protein-2, which triggers neutrophilic infiltration and tissue injury (1, 27, 34). Thus persistent NF-κB activation during the early phase of inflammation amplifies inflammatory response in vivo.. In contrast, transient activation of NF-κB before inflammatory stimulation results in the anti-inflammatory response. For example, several investigators (41, 54, 56) have found that transient activation of NF-κB is required for the heart to tolerate ischemia-reperfusion-induced ...
Time-dependent induction of connected cells by overexpression of cdc5ΔN. (A) Strain KLY1083 expressing three copies of GAL1-EGFP-cdc5ΔN homogeneously induced
floating THP-1 cells - medium change advice - posted in Tissue and Cell Culture: Hello, I was adamantly asked to culture THP-1 cells and did so, by thawing a cryovial and seeding the cells in a 25 cm flask. 3 days later 99% of cells are floating, which I reckon is OK. Isnt it? So, do I need to change the medium before they reach ~8x 10^5 cells/ml and are ready to be passed? Since these are floating cells, how do I do that? Also, would a 1/3 split ratio be OK for these cells? Th...
1) The main issue with this paper is this: these cell lines, although originally human, are all immortalized cancer cell lines characterised by markedly different biological properties when compared to normal human cells of the same tissue/cell type. They cant be readily used a surrogates for normal human tissue/cell types. None were primary cells nor were any even from recently acquired tissue samples from biopsies etc. People have infected primary human airway epithelial cultures with hCoV-EMC - so this can be done successfully - , although it would be more difficult for other tissue types as these cultures havent been developed. Some of these cell lines used may by chance lack key viral repressors of infection present in normal primary cells, which could skew results from cell culture infection experiments. Plus, a human tissue is not just a single cell type - they are composed of diverse kinds of cells that could together behave much, much differently than cell lines in culture ...
Accepted name: raucaffricine β-glucosidase. Reaction: raucaffricine + H2O = D-glucose + vomilenine. For diagram click here.. Other name(s): raucaffricine β-D-glucosidase; raucaffricine glucosidase. Systematic name: raucaffricine β-D-glucohydrolase. Comments: Highly specific; some other ajmalan glucoside alkaloids are hydrolysed, but more slowly.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 102925-37-1. References:. 1. Schübel, H., Stöckigt, J., Feicht, R. and Simon, H. Partial-purification and characterization of raucaffricine β-D-glucosidase from plant cell-suspension cultures of Rauwolfia serpentina benth. Helv. Chim. Acta 69 (1986) 538-547.. ...
Increasing numbers of studies have demonstrated that PGE1 plays an important role in determining the survival of cells exposed to H/R conditions. PGE1 treatment of liver sinusoidal endothelial cells increases their survival and resistance to apoptosis during H/R injury by suppressing NO production and MMP release [31]. Interestingly, a previous study by Ma et al. [32] reported that the total number of cardiomyocytes was decreased in an H/R group when compared with a group of cardiomyocytes pretreated with PGE1, and the mechanism for that effect probably involved a decrease in Bax expression and an increase in Bcl-2 expression. Caspase 3, which is downstream of Bcl-2 and Bax, is considered to be a critical protease required for apoptosis [39]. In the present study, we observed that inhibition of apoptosis by PGE1 in H/R injured cells involved the inactivation of cleaved-caspase-3. Our results support a model in which PGE1 inhibits apoptosis in cardiomyocytes, at least in part, via a ...
Finally, our article on human and mouse neurons has been published! The picture above shows the neurons of a human and a mouse. Human and mouse neurons are similar overall, but they also have…
CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics ...
483279889 - EP 3108256 A4 2017-10-04 - METHOD OF DETECTING CANCER - [origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.[origin: WO2015120523A1] The disclosure provides methods for resolving an inconclusive cytological assessment of clinically relevant cells in a sample obtained from a patient based on the cytological detection of antibody binding to telomerase in clinically relevant cells in the sample, wherein binding of the antibody to clinically relevant cells indicates the presence of malignant or cancerous cells.
Results ICAM-1 expression was significantly up-regulated upon the introduction of TNF-α under all conditions in HUVEC (figure 1). However, baseline expression was increased when co-cultured with both NHDF (2.0 vs 1.3, p,0.001) and HUASMC (6.5 vs 1.3, p,0.001). This meant that ICAM-1 expression at 12 hours was also significantly higher in co-culture with NHDF (8.3 vs 5.2, p,0.001) and HUASMC (11.0 vs 5.2, p,0.001). Moreover, there was a moderate relationship between HUVEC ICAM-1 expression and the cell ratio when in co-culture with NHDF, where decreasing NHDF resulted in decreased ICAM-1 in HUVEC (R2=0.45).. TNF-α caused an increase in ICAM-1 expression in NHDF under monoculture conditions (Fig. 2); this up-regulation was significantly reduced in co-culture conditions with HUVEC (1.7 vs 5.1, p,0.001). A similar trend was observed when in co-culture with HUASMC (2.7 vs 5.1, p,0.001), except the baseline expression of ICAM-1 was also increased (2.8 vs 1.1, p,0.001).. Constitutive production of ...
Mouse IL-4 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
Mouse IL-10 ELISA kit (5 plate). The U-CyTech sandwich ELISA kits have been developed for cytokine determinations in culture supernatant, serum and plasma. The kit is supplied with all necessary reagents to perform 480 ELISA determinations. Cytokines are a group of regulatory proteins critically involved in many physiological processes such as immune recognition, cell differentiation and cell proliferation. They have been identified in many vertebrate species and are produced by a variety of different cell types. Cytokines are usually produced transiently and locally, acting in a paracrine or autocrine manner. They interact with high affinity cell surface receptors specific for each cytokine or cytokine group and are active at very low concentrations mostly in the picogram range. It is well known now that the type of an antigen-specific immune response largely depends on the selection or preferential activation of defined CDM|sub|4|sup|+ T cell subsets (i.e. Th1 and Th2). Activation of these subsets is
2010) (Fig. 1A). Fibroblasts were seeded at 1.5 × 105 cells/filter and HUVEC were seeded at 1.0 × 105 cells/filter to yield confluent monolayers within 24 h. After 24 h, culture media were removed and the 24-well inserts were fitted into the 12-well inserts, with 200 μl fibroblast medium added to the surface of each filter and 1.5 ml to the lower chamber. Cells were co-cultured together for 48 h, with 100 U/ml TNF alpha (R&D Systems, Abingdon, UK) in combination with 10 ng/ml IFN gamma (Peprotech Inc., London, UK) added for the second 24 h when desired. For. comparison, parallel cultures of HUVEC or fibroblasts were maintained alone on their Selleckchem AC220 original filters. To form collagen gels, ice-cold rat-tail type 1 collagen PD-166866 cell line dissolved in acetic acid (2.15 mg/ml; First Link Ltd, West Midlands, UK) was mixed with ice cold 10 × concentrated M199 in the ratio 830:170 and the pH was neutralised by addition of ice cold 1 N NaOH. For each 1 ml of gel, 160 μl FCS was ...
You know its funny that you mention tube like structures...... do they look fairly long and rectangular? I have something growing in tissue culture that looks like that. These cells started off as hemangiomas. We used collangenase to break it up, and are growing these cells in DMEM with added penicillin/streptomyacin and fetal bovine serum. Ive seen quite a few of these, one person thinks they look like myocytes....heart muscle cells in particular, they arent beating or anythying though. And if that is what they are, id be really really surprised. Most of the cells look like fibroblasts (Im thinking that what look like fibroblasts might be pericytes) with some scattered possible endothelial cells. We transfected a few dishes with a vector to induce division, but we dont see these cells in any of the tissue culture dishes containing the transformed cells. I wonder if we are looking at the same thing. Ive ordered a camera for our inverted scope, ill have to take a picture when it comes in. ...
The characterization was performed using overnight cultures of the corresponding part. The optical density of the cell cultures was adjusted to 0.4 and 50 µl of the culture sample was pipette onto a microscope slide and a coverslip was placed over it. A 100X/1.3 Oil immersion objective was used for the light microscopy imaging and filters necessary for CFP fluorescence detection was set. An initial live imaging was done in transmitted light mode to find a field of view with maximum number of cells. The same was then used for capturing the fluorescence signal of the CFP. The same procedure was repeated several times in order to quantify the data. If we need to observe more number of cells under a given field of view, it is recommended to spin down the cell culture and use the resuspended pellet for increased cell density.. ...
The characterization was performed using overnight cultures of the corresponding part. The optical density of the cell cultures was adjusted to 0.4 and 50 µl of the culture sample was pipetted onto a microscope slide and a coverslip was placed over it. A 100X/1.3 Oil immersion objective was used for the light microscopy imaging and filters necessary for CFP fluorescence detection was set. An initial live imaging was done in transmitted light mode to find a field of view with maximum number of cells. The same was then used for capturing the fluorescence signal of the CFP. The same procedure was repeated several times in order to quantify the data. If we need to observe more number of cells under a given field of view, it is recommended to spin down the cell culture and use the resuspended pellet for increased cell density.. ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Microcarrier Cell ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... A 3D cell culture model showed cell growth similar to that of in vivo than did a monolayer culture, and all three cultures were ...
Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment ... usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass ... dependent cell types. v t e (Articles lacking sources from December 2009, All articles lacking sources, Laboratory equipment, ...
The Sf9 cell line is one of the most commonly used lines in insect cell culture. Drugmand, Jean-Christophe; Schneider, Yves- ... v t e (Cell culture techniques, Biopharmaceuticals, All stub articles, Pharmacology stubs). ... Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are ... The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are ...
... and cell loading would need to be added for the cell culture maintenance. Perfusion in microfluidic cell culture is important ... Gel free 3D cell cultures have been developed to allow cells to grow in either a cell dense environment or an ECM poor ... Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... These reduced cell numbers make studying non-dividing or slow dividing cells (e.g., stem cells) easier than traditional culture ...
Cell culture Cell lines Cell culture assay Hydrogel Madin-Darby Canine Kidney cell line Microphysiometry Fey S, Wrzesinski K ( ... cell-cell/cell-matrix interaction, and/or cell migration, but the available assays are not optimized for 3D cell culturing. ... Lastly, 3D cell cultures have greater stability and longer lifespans than cell cultures in 2D. This means that they are more ... Furthermore, 3D cell cultures provide more accurate depiction of cell polarization, since in 2D, the cells can only be ...
... through the inhibition of cell metabolic pathways. Cell culture evaluations are the precursor to whole animal studies and are a ... A cell culture assay is any method used to assess the cytotoxicity of a material. This refers to the in vitro assessment of a ... Cell culture assays are standardized by ASTM, ISO, and BSI (British Standards Institution.) Biology portal Technology portal ... "A cell culture assay for the detection of cardiotoxicity". Journal of Pharmacological Methods. 25 (2): 133-45. doi:10.1016/0160 ...
... is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture ... More specific terms include organotypic culture, tissue slices and explants. 3D cell culture Induced pluripotent stem cell ... primary cell cultures did not become widespread until the 2000s. These cultures present several advantages over cell lines, ... In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized ...
The cell culture bioreactor-known commercially as the Rotary Cell Culture System (RCCS) -- boasts several advantages that ... Lab-grown cell cultures tend to be small, flat and two-dimensional, unlike normal cultures. But tissues grown in the RCCS are ... The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. In the ... A closed tubular cylinder forms the system's cell culture chamber, which is filled with a liquid medium in which cells grow on ...
Another type of material that is used for open-microfluidic cell culturing is paper-based microfluidics. Cell culturing on ... such as quorum sensing or co-culturing of several types of cells. A majority of cell culturing has been carried out by ... The adsorption of small biological molecules from cell culturing samples as well as the release of oligomers into the culture ... where the device is open for the introduction and culturing of cells, and can be sealed prior to analysis. Cells and proteins ...
Air liquid interface cell culture (ALI) is a method of cell culture by which basal stem cells are grown with their basal ... This method of cell culture aims to be used to study fundamental aspects of the respiratory epithelium, such as cell-to-cell ... The cell culture method used involves the isolation of cells into culturing with growth factors to grow over time. Once the ... Air-liquid interface cell culture compares to standard cell culture practices by specifically aiming to restore the ...
3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high- ... Because of the ability of 3D cell culturing by magnetic levitation to bring cells together, co-culturing different cell types ... Standard monolayer cell culturing on tissue culture plastic has notably improved our understanding of basic cell biology, but ... Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio- ...
The European Collection of Authenticated Cell Cultures houses and supplies cell lines. It is part of the Culture Collections of ... ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 species ... the culture collections website alongside a range of services such as Mycoplasma testing and training courses for cell culture ... Culture Collections Official home page v t e (Use dmy dates from April 2022, Articles with topics of unclear notability from ...
Different cell lines and cell types have been cultured in NFC, including e.g. differentiation of human hepatic cells to ... like cell culturing. GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. NFC fiber network structure ... functional organotypic cultures, and proliferation of human pluripotent stem cells. Organotypic liver cell cultures can be used ... Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. As plant based material, it ...
Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing ... While SILAC had been mostly used in studying eukaryotic cells and cell cultures, it had been recently employed in bacteria and ... Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects ... Amanchy R, Kalume DE, Pandey A (January 2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying ...
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed ... Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding ... and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue ... Cells. 6 (1): 4. doi:10.3390/cells6010004. PMC 5371869. PMID 28157165. (Cell culture). ...
Dawn news election cells. Dawn news election cells. Retrieved 21 February 2015.- Hoodbhoy, Pervez. "Noam Chomsky interviewed by ... The Culture of Pakistan (Urdu: پاکستانی ثقافت Pākistāni S̱aqāfat) is very unique in terms of its social values revolving around ... The consumption of tea in Pakistan, called chai (چائے), in Urdu, is of central significance to Pakistani culture. It is one of ... With the beginning of the Indus civilization around the middle of the 3rd millennium BCE, an advanced urban culture developed ...
Marchi, Nina (2022). "The genomic origins of the world's first farmers". Cell. 185 (11): 1842-1859. doi:10.1016/j.cell.2022.04. ... Neolithic cultures of Europe, Archaeological cultures in Austria, Archaeological cultures in Belgium, Archaeological cultures ... Archaeological cultures in Germany, Archaeological cultures in Hungary, Archaeological cultures in Moldova, Archaeological ... cultures in the Netherlands, Archaeological cultures in Poland, Archaeological cultures in Romania, Archaeological cultures in ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. ISSN 1097-4172. "History of Buratia Culture", Ulan-Ude, 2003 ... Archaeological cultures of Northern Asia, Bronze Age cultures of Asia, Iron Age cultures of Asia, Archaeological cultures of ... The Slab-Grave culture is an archaeological culture of the Late Bronze Age and Early Iron Age Mongols. The ethnogenesis of ... The slab-grave culture art has many common features with cultures of Southern Siberia: Karasuk, Tagar, and others. Thousands of ...
Cell. 184 (18): 4612-4625.e14. doi:10.1016/j.cell.2021.07.013. ISSN 0092-8674. PMC 8445022. PMID 34352227. Broushaki, F; Thomas ... Epipalaeolithic cultures, Archaeological cultures in Israel, Archaeological cultures in Jordan, Archaeological cultures in ... Natufian culture, Industries (archaeology), Archaeological cultures of West Asia, Archaeological cultures of the Near East, ... The Natufian culture (/nəˈtuːfiən/) is a Late Epipaleolithic archaeological culture of the Levant, dating to around 15,000 to ...
Cell. 163 (3): 571-582. doi:10.1016/j.cell.2015.10.009. PMC 4644222. PMID 26496604., S14-15. This sample is marked "RISE505". ... 2009) published a study of the ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and the ... They differ slightly from the skulls of the Yamnaya culture, Poltavka culture, Catacomb culture and Potapovka culture, which ... Andronovo skulls are very similar to those of the preceding Fatyanovo-Balanovo culture, Abashevo culture and Sintashta culture ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. Savinov D.G., Early ... Archaeological cultures in Russia, Iron Age cultures of Asia, Iranian archaeological cultures, Scytho-Siberian world). ... Archaeological cultures of Central Asia, Archaeological cultures of Northern Asia, Archaeological cultures in Kazakhstan, ... The culture is a continuation of the nomadic Pazyryk culture in the area of Altai Mountains in South Siberia. The monuments of ...
Cell Press. 27 (14): 2185-2193. doi:10.1016/j.cub.2017.06.022. PMID 28712569. Zvelebil, Marek (2004). "Pitted Ware And Related ... The Comb Ceramic culture or Pit-Comb Ware culture, often abbreviated as CCC or PCW, was a northeast European culture ... Pitted Ware culture Dnieper-Donets culture Rzucewo culture Pre-Finno-Ugric substrate Zvelebil 2004, pp. 431-435. Helle, Knut; ... It would include the Narva culture of Estonia and the Sperrings culture in Finland, among others. They are thought to have been ...
Cell Press. 19 (20): 1758-62. doi:10.1016/j.cub.2009.09.017. PMID 19781941. S2CID 9487217. Malmström, Helena; et al. (January ... Western Linear Pottery culture Pit-Comb Ware culture Cardium Pottery culture Vlaardingen culture Prehistory and protohistory of ... Archaeological cultures of Central Europe, Archaeological cultures of Northern Europe, Archaeological cultures in the Czech ... Archaeological cultures in Germany, Archaeological cultures in the Netherlands, Archaeological cultures in Norway, ...
Cell Press. 27 (4): 576-582. doi:10.1016/j.cub.2016.12.060. PMC 5321670. PMID 28162894. Mathieson, Iain (February 21, 2018). " ... Mesolithic cultures of Europe, Archaeological cultures in Belarus, Archaeological cultures in Estonia, Archaeological cultures ... The oldest known settlement of the Kunda culture in Estonia is Pulli. The Kunda culture was succeeded by the Narva culture, who ... The Kunda culture appears to have undergone a transition from the Palaeolithic Swiderian culture located previously over much ...
Cell Press. 27 (14): 2185-2193. doi:10.1016/j.cub.2017.06.022. PMID 28712569. Overview of Neolithic sites on Narva River in ... Mesolithic cultures of Europe, Archaeological cultures in Estonia, Archaeological cultures in Latvia, Archaeological cultures ... A successor of the Mesolithic Kunda culture, Narva culture continued up to the start of the Bronze Age. The culture spanned ... Pitted Ware culture Rzucewo culture Dnieper-Donets culture Zinkevičius, Zigmas; Luchtanas, Aleksiejus; Česnys, Gintautas (2007 ...
Cell Press. 19 (20): 1758-62. doi:10.1016/j.cub.2009.09.017. PMID 19781941. Malmström, Helena (30 March 2010). "High frequency ... Germanic substrate hypothesis Narva culture Rzucewo culture Dnieper-Donets culture Swifterbant culture Zvejnieki burial ground ... From around 2,800 BC, the Pitted Ware culture co-existed for some time with the Battle Axe culture and the Single Grave culture ... During its initial years, the Pitted Ware culture co-existed with the Funnelbeaker culture. Although the two cultures exchanged ...
Cell Press. 25 (4): 104094. doi:10.1016/j.isci.2022.104094. PMC 8983337. PMID 35402880. Garrow, Duncan (ed), Rethinking Celtic ... La Tène culture, Celtic archaeological cultures, Iron Age cultures of Europe, Archaeological cultures of Central Europe, ... Gaulish culture quickly assimilated to Roman culture, giving rise to the hybrid Gallo-Roman culture of Late Antiquity. The ... Archaeological cultures of Southern Europe, Archaeological cultures of Southwestern Europe, Archaeological cultures of Western ...
Cell Press. 25 (4): 104094. Bibcode:2022iSci...25j4094F. doi:10.1016/j.isci.2022.104094. PMC 8983337. PMID 35402880. Leskovar, ... Archaeological cultures of West Asia, Iron Age cultures of Europe, Celtic archaeological cultures, Iron Age of Slovenia, ... Archaeological cultures of Central Europe, Archaeological cultures of Southeastern Europe, Archaeological cultures of Southern ... Archaeological cultures in Bulgaria, Archaeological cultures in Croatia, Archaeological cultures in the Czech Republic, ...
Cell Press. 27 (14): 2185-2193. doi:10.1016/j.cub.2017.06.022. PMID 28712569. Sjögren, Karl-Göran (2020), "Kinship and social ... Funnelbeaker culture Fatyanovo-Balanovo culture Middle Dnieper culture Bell Beaker culture Ertebølle culture Gimbutas uses the ... the Narva culture, the Funnelbeaker culture, the Linear Pottery culture, the Cardium pottery culture, the Vinča culture, the ... 2019) found the Sintashta culture, the Potapovka culture, the Andronovo culture and the Srubnaya culture to be closely related ...
2. High cell density (High cell concentration)[1] In a batch culture, to achieve very high cell concentrations, e.g. 50-100 g ... An alternative description of the method is that of a culture in which "a base medium supports initial cell culture and a feed ... When a specified chemical compound exists in the culture liquid, the compound (or its metabolite) in the cells combines as co- ... L. Yee, Harvey W. Blanch: Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli. Bio/ ...
It is known that the most frequently colonized sites are epithelial cell surfaces and red and white blood cells inside of the ... There have been frequent unsuccessful attempts at isolating a culture of M. incognitus because it is more fastidious in ... Since M. incognitus is a mycoplasma, it does not have a cell wall, which means that it is naturally immune to many different ... This mycoplasma acts by entering into the individual cells of the body where it can lie dormant for 10, 20, or 30 years. If the ...
"Effect of 5-phosphoribosyl-a-pyrophosphate on de novo pyrimidine biosynthesis in cultured Ehrlich ascites cells made permeable ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. ... Lin T, Suttle DP (May 1995). "UMP synthase activity expressed in deficient hamster cells by separate transferase and ... Somatic Cell Genetics. 9 (3): 359-74. doi:10.1007/BF01539144. PMID 6574608. S2CID 29498380. McClard RW, Black MJ, Livingstone ...
Test subjects included the crew, Japanese koi fish (carp), cultured animal and plant cells, chicken embryos, fruit flies, fungi ... Life sciences included experiments on human health, cell separation and biology, developmental biology, animal and human ...
There are things to learn from cultures of the past, but the current culture is the most important. -Ryan Bundy On January 19, ... Other cell phone video footage shot by Ryan Bundy, another passenger, also showed Finicum taunting officers and daring them to ... "Ryan Bundy's cell phone video of moments before and after Finicum shooting released". Portland, Oregon: KATU. April 5, 2016. ... Shawna Cox, a passenger in Finicum's truck, recorded cell phone video of Finicum shouting to police that he intended to ignore ...
Cell phones are also very common among all three groups today. Text messaging has made cell phones particularly useful for ... There is a wide range of leadership cultures and styles between the three main colony varieties. In some cases very dominant ...
... "underpinned by a pervasive drug culture", but said the prison was well led, staff appeared committed and there was an apparent ... to provide additional single prison cell accommodation to help counter the spread of coronavirus. "Humber Prison information". ...
p. 8. ISBN 0-8493-6252-0. "6-(γ,γ-Dimethylallylamino)purine BioReagent, suitable for plant cell culture, 1 mg/mL". ... It promotes growth of lateral buds and when sprayed on meristems stimulates cell division to produce bushier plants.[citation ...
In June 2003, Carlos published a collection of writings from his jail cell. The book, whose title translates as Revolutionary ... "Carlos director Olivier Assayas on the terrorist who became a pop culture icon". The Guardian. London. Archived from the ... a supporter of the imprisoned Red Army Faction and a member of the Revolutionary Cells, and Gabriele Kröcher-Tiedemann, from ... Venezuelan president Hugo Chávez had a sporadic correspondence with Carlos from the latter's prison cell in France. Chávez sent ...
The Israeli, Spanish, and an Equatorial Africa pavilion (blending elements of the cultures of countries such as Kenya and Zaire ... The attraction is an electronic scavenger hunt that has guests using special "Kimmunicators" (in actuality, customized cell ... World Showcase is the park's largest neighborhood, reminiscent of a permanent world's fair dedicated to represent the culture, ... while others wanted it to showcase international cultures and customs. At one point, a model of the futuristic park was pushed ...
DIC is used for imaging live and unstained biological samples, such as a smear from a tissue culture or individual water borne ... a cell in water only has a refractive index difference of around 0.05. This small phase difference is important for the correct ...
If the stem cells and sebaceous gland are destroyed, there is then no possibility for regeneration of the hair follicle, and ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ... The goal of treatment is to decrease or eliminate the lymphocytic inflammatory cells that are attacking and destroying the hair ... However, all cicatricial alopecias involve inflammation directed at the upper part of the hair follicle where the stem cells ...
... the zooxanthellate jellyfish Cotylorhiza tuberculata modulates gap junction intercellular communication in human cell cultures ... C. tuberculata have been studied due to their specified cytotoxicity in regards to certain breast cancer cells, which is made ... Since a main objective in cancer research is to create treatments that selectively kill malignant cells while leaving healthy ...
Since the cells contains hydrogenase, it has become a useful material in clean energy production.[citation needed] Fujisawa T, ... The temperature optimum for this organism is around 35 °C. Based on environmental conditions, culture medium often has a pH ...
"Epilepsy not due to injured brain cells is caused by the suppression of hate in childhood". While in D.C., Thompson undertook a ... "much information has been obtained about the culture of the vanished Chamorros, a flourishing race at the time of Magellan's ...
... replication properties and cell culture tropism from the swabs. They made available the virus to the wider scientific community ... Coronaviruses manipulate the cell cycle of the host cell through various mechanisms. In several coronaviruses, including SARS- ... N also has additional functions in manipulating the cell cycle of the host cell. The N protein is highly immunogenic and ... "Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals". Cell. 181 (7 ...
Academic pressure in East Asian culture, Chinese culture, Chinese inventions, Imperial examination, Confucian education, Civil ... When he finally enters his cell and, along with the other candidates, stretches his neck to peer out, he is just like the larva ... This common culture helped to unify the empire, and the ideal of achievement by merit gave legitimacy to imperial rule. The ... The facilities provided for the examinee consisted of an isolated room or cell with a makeshift bed, desk, and bench. Each ...
ISBN 978-0-674-07405-7. Blom, Philipp (2008). The Vertigo Years: Change and Culture in the West, 1900-1914. Toronto: McClelland ... accused the administration in July 2007 of political interference and muzzling him on key issues like embryonic stem cell ...
"Western Culture" credit requirements to "Cultures, Ideas, and Values" in an attempt to encompass non-Western cultures. Kennedy ... demonstrating some of the connection principles among nerve cells that impose the sequences underlying a behavioral event. ...
An unspecific cell culture toxicity test and a feeding test on mice revealed no toxicity in C. gentilis. List of deadly fungi " ...
An interviewer from a tight culture might view the normal behaviors of a loose cultured interviewee as signs of a poor moral ... Items like cell phones, a cup of coffee, and chewing gum are not recommended to bring to an interview, as it can lead to the ... This is why it is important to understand how and why the best answer in one culture is not the best elsewhere. It might even ... Tight cultures have strong social norms and adherence coupled with low tolerance for behavior that deviates from those norms, ...
... and causes cell death in cultured cells". J. Biol. Chem. 275 (4): 2647-53. doi:10.1074/jbc.275.4.2647. PMID 10644725. Li PF, Li ... "Calcium binding of ARC mediates regulation of caspase 8 and cell death". Mol. Cell. Biol. 24 (22): 9763-70. doi:10.1128/MCB. ... Cell. 15 (6): 901-12. doi:10.1016/j.molcel.2004.08.020. PMID 15383280. Jo DG, Jun JI, Chang JW, Hong YM, Song S, Cho DH, Shim ... J. Physiol., Cell Physiol. 284 (6): C1405-10. doi:10.1152/ajpcell.00279.2002. PMID 12734105. Nam YJ, Mani K, Ashton AW, Peng CF ...
Meyer, P.S., Du Preez, J.C., Kilian, S.G. (1992). "Effect of temperature and pH on Candida blankii in chemostat culture". World ... A diploid isolate of C. blankii had an observed "potential for use in single cell protein production from hemicellulose ...
From his cell, Humala reportedly orchestrated the impeachment process with members of congress and his UPP supporters. Edgar ... and for violating the principles of good administration and budget justice by hiring Richard Swing in the Ministry of Culture. ...
At the jail, his cell-block neighbor was Michael Slager, the former North Charleston police officer charged with murder after ... this trope is related to a myth of Southern culture, because in fact, African-American women had been much more frequently ... in a country known for its diversity and blending of various cultures." Many national Muslim organizations and individual imams ...
... and she was imprisoned in an underground cell at Château Gaillard castle. Louis succeeded to the throne later that year after ... The affair would also have an impact in European culture. Scholars studying the theme of courtly love have observed that the ...
Christopher medal in Nicolas' bag when she found her cell phone, and knowing that the only way he could have that was if he was ... Culture Map Houston. Retrieved August 10, 2012. Dallas both TV series every intro, retrieved 2021-10-12 Hibberd, James (July 8 ...
... the forced overexpression of CMTM5-v1 in cultured DU145 cells (a human prostate cancer cell line) reduces, while the forced ... The forced over expression of CMTM5-v1 in Huh7 human hepatic cells also inhibited the ability of these cells to grow in a mouse ... Cai B, Xiao Y, Li Y, Zheng S (August 2017). "CMTM5 inhibits renal cancer cell growth through inducing cell-cycle arrest and ... the forced overexpression of the CMTM5 gene inhibited the proliferation and migration of cultured human endothelial cells, ...
CNN reported that students, often teenagers, sell sex so they can buy consumer goods such as cell phones and clothes. Hong Kong ... Rethinking Popular Culture in the Republican Period, Oxford University Press, ISBN 9780199282715 Hodge, Peter (1980), Community ...
The S-layer is made of a cell-surface glycoprotein that accounts for approximately 50% of the cell surface proteins. These ... H. salinarum is as easy to culture as E. coli and serves as an excellent model system. Methods for gene replacement and ... The primary role of bacterioruberin in the cell is to protect against DNA damage incurred by UV light. This protection is not, ... It is able to protect the cell from reactive oxygen species produced from exposure to UV by acting as a target. The ...
Stephen Murray died in San Francisco, California on August 27, 2019 of complications of a B-cell lymphoma. Murray, Stephen O ( ... transgender and queer culture, GLBTQ and other reference volumes. He was a regular contributor at and ... Culture, history, and literature, New York University Press, ISBN 978-0-8147-7468-7 Murray, Stephen O; Roscoe, Will (1998), Boy ... and Queer Culture. (Articles with short description, Short description is different from Wikidata, Articles with hCards, ...
... has transformed cell-based assays in drug discovery and basic biology by conferring physiologic relevance to in vitro cell- ... Starting Cells Make a Difference. 3D cell cultures have been grown from various cells, including transformed cell lines and ... will require cell culture tools up to the task. Industry collaborations assure that tools for 3D cell culture will continue to ... When cultured under identical HUB protocols adult progenitor cells give rise to organoids with exactly the same cells in the ...
... complete with recipes and protocols.Because neurons and glia in culture are remarkably similar to those in... ... A do-it-yourself manual for culturing nerve cells, ... Culturing Nerve Cells, second edition Edited by Gary Banker and ... experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture ... This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also ...
Cell Culture. InvivoGen provides a wide range of high-quality reagents for tissue and cell culture applications, including ... Cell Transfection Antimicrobial Agents Selective Antibiotics Reporter Detection Kits Endotoxin Detection Mycoplasma Detection ...
... cowboy culture of unregulated, direct-to-consumer marketing of questionable treatments. ... Cite this: DTC Stem Cell Marketing Common in US in Cowboy Culture - Medscape - Jun 30, 2016. ... After using phrases such as "stem cell therapy" and "stem cell treatment" to identify the companies, they visited each ... For example, current discussions of stem cell therapies usually concern "stem cell tourism" to centers outside the United ...
But as 3D cell cultures rise in popularity, they bring unique challenges that scientists are working to fix. ... Many researchers are moving away from using cells grown in monolayers, which do not represent their growth in the body. ... Good cell culture practice is essential. Keeping organoid cultures alive and healthy can be challenging - particularly over ... Entering the third dimension of cell culture. Many researchers are moving away from using cells grown in monolayers, which do ...
Use of cell culture in virology for developing countries in the South-East Asia Region  ... Browsing Regional Publications by Subject "Cell Culture Techniques". 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R ...
Both mouse and human intestinal stem cells can be cultured in medium containing the stem-cell-niche factors WNT, R-spondin, ... Crypt stem cells represent the cells of origin for intestinal neoplasia. ... Both mouse and human intestinal stem cells can be cultured in medium containing the stem-cell-niche factors WNT, R-spondin, ... Sequential cancer mutations in cultured human intestinal stem cells Nature. 2015 May 7;521(7550):43-7. doi: 10.1038/nature14415 ...
CELL 1. a small room in a convent or monastery; 2. the separate individual room of a monk or nun; 3. the cottage of a ... CELL. 1. a small room in a convent or monastery; 2. the separate individual room of a monk or nun; 3. the cottage of a ...
Les cultures cellulaires comme substrats pour la production des vaccins antigrippaux : mémorandum dune réunion de lOMS  ... Plasmodium falciparum : continuous cultivation of erythrocyte stages in plasma-free culture medium / by G. P. Willet, C. J. ...
... and multiply cells for cell therapy. To date, standard cell culture comprises that cells live in a soup of nutrients on the ... Cell culture is the process of taking cells out of the body and growing them in an artificial environment. It is inescapable to ... and multiply cells for cell therapy. To date, standard cell culture comprises that cells live in a soup of nutrients on the ... A second aspect of the project consists in quantifying the benefits of culturing cells on our innovative cell culture system. ...
Here you will find a complete list of all scientific publications. ...
Cell Culture Grade Antibodies : we have 167 product. *List alphabetically by product name : ...
Getting-to-Market barriers for cell-cultured meat discussed in new GAO report. By Dan Flynn on May 11, 2020. ... Continue Reading Getting-to-Market barriers for cell-cultured meat discussed in new GAO report ... "could strengthen existing efforts to prepare for oversight of cell-cultured meat." ... Instead, its happening in labs where cells taken from live chickens, cows, or other livestock are grown… ...
... debris in culture media, cell detachment or clumping, slow growth, and low viability. ... Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, ... Stem Cell Culture. An overview of different stem cell types, stem cell culture basics and stem cell applications in basic ... Cell culture antibiotic selection guide, including antibiotics for mammalian cell culture, plant cell culture, and antibiotic ...
FDA Issues First Approval for Cell-Cultured Chicken. Cultivated meat producer Upside Foods received regulatory approval from ... blending small quantities of lab-grown cells into primarily plant-based products. While affordable, 100% cultivated … ...
... paving the way for manufacturing cell cultured meat and breeding enhanced livestock. ... Scientists have for the first time obtained stem cells from livestock that grow under chemically defined conditions, ... The chemically defined conditions are growth medium suitable for the in vitro cell culture of animal cells in which all of the ... Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS ...
Even though large-scale cell culture is more of an industry than a craft, biomanufacturers shouldnt overlook scale-down ... Tricks of the Trade for Cell Culture Optimization. Cell Culture Knowhow Encompasses Scale-Down Modeling, Inline Sampling, and ... At the Optimizing Cell Culture Technology conference, a presentation entitled "CRISPR Tools for CHO Cell Engineering" was ... which tend to increase in expression under the higher stress conditions of cell culture and activate programmed cell death ...
... a certain epigenetic mark vanishes from mouse cells. ... Culturing Changes Cells. Within days of their transfer to a ... "What we would like to know is, does the same phenomenon occur in human cancer cells taken out of tumors and put in culture, and ... 5hmC (red staining) is lost after mouse skin cells persist in culture.S. PENNINGS LABIn vitro experimentation is a necessary, ... Meehans group found that Tet gene expression declined as cells spent more time in culture. Yun Nancy Huang, an epigenetics ...
Cell Culture job in Cambridge, MA with Pfizer. Apply Today. ... Cell Culture. Employer. Pfizer Location. Cambridge, MA. Start ... Experience working with bacterial and mammalian cell lines and cell culture, transfection, and cell-based assays ... is expected to have a strong background in cell and molecular biology with experience in bacterial and mammalian cell culture. ... The candidate will be responsible for maintaining cells for plasmid production and to drive efforts in developing novel cell- ...
... are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cells. ... C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. ... continuously cultured carcinoma cell line used for tissue cultures). Cytologic diagnosis also is used to evaluate endocervical ... Cytology and Cell Culture. Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through ...
We conclude that lyso-PC can induce growth factor gene expression in cultured endothelial cells and thus may contribute to the ... Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells.. ... Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells.. ... in cultured human endothelial cells. Lyso-PC did not upregulate the expression of certain other inducible endothelial genes, ...
Other topic areas which will be featured at Cell Culture 2012 are the challenges involved with preventing cell line cross ... Cell Culture. Date: Wednesday, 27 June 2012 - Thursday, 28 June 2012. Location: London, England. ... Our inaugural Cell Culture Europe 2012 promises to deliver an array of presentations on the latest techniques and technologies ... as well as novel methods in the use of 3D cell cultures.. Running alongside the conference will be an exhibition covering the ...
The utility model discloses a cell culture medium filter equipment, including casing, base, filter screen and straight-bar, the ... The variety of cell culture media is various, the cell culture modes are also many, how to correctly use the culture media and ... CN209890624U - Cell culture medium filter equipment - Google Patents. Cell culture medium filter equipment Download PDF Info. ... Cell culture medium filter equipment. Technical Field. The utility model relates to a cell culture equipment technical field ...
For this Ask the Expert Session, The Cell Culture Dish assembled a team of experts to answer questions on contamination and ... Mycoplasma contamination is indeed a key factor to evaluate during a cell culture projects, as it can influence cell behavior, ... A first step when starting a culture is cell authentication to determine identity and species of cells ... Periodically check your cells for morphology or genetic changes. Cell authentication to determine identity and species of cells ...
Our ethically sourced human primary cell portfolio includes fresh and cryopreserved products from peripheral blood and cord ... Human Primary Cells. It All Starts With the Right Cells View Products , ... Human Peripheral Blood Pan-T Cells, Fresh Freshly isolated human peripheral blood pan-T cells. ... Human Peripheral Blood NK Cells, Fresh Freshly isolated human peripheral blood NK cells. ...
... and 36 after dose 1 of a 2-dose primary series of inactivated Vero cell culture--derived JE vaccine (JE-VC [manufactured as ... The updated recommendations included information on use of a new inactivated, Vero cell culture--derived JE vaccine (JE-VC [ ... Recommendations for Use of a Booster Dose of Inactivated Vero Cell Culture-Derived Japanese Encephalitis Vaccine --- Advisory ... and month 12 after a booster dose of inactivated Vero cell culture--derived Japanese encephalitis vaccine (JE-VC [manufactured ...
The cells showed an apparent increase in size, diffuse cell rounding, and a refringent aspect (Figure 1B). In Hep-2C cells, the ... Infection of Cultured Human and Monkey Cell Lines with Extract of Penaeid Shrimp Infected with Taura Syndrome Virus On This ... refringent cells. Some cells remained isolated but with altered morphology (Figure 1F). RD, Hep-2C, and BGM cells injected with ... Infection of Cultured Human and Monkey Cell Lines with Extract of Penaeid Shrimp Infected with Taura Syndrome Virus. Emerging ...
The new module handles tissue cultures in microplate-sized, automation friendly cell culture flasks, which have been introduced ... Automated cell culture from Tecan. Tecan, one of the worlds leading players in the growing life sciences industry, has ... splitting and seeding of adherent cell cultures. The Flask Flipper module assists the cell harvesting process by shaking, ... The module eliminates manually tedious cell culturing processes by automating the harvest, passage, ...
A cell culture vessel designed by Taiwanese company CESCO Bioengineering can produce the same amount of cells in a single 500ml ... Related tags: Cells, Protein, Cell culture, Stem cell A cell culture vessel designed by Taiwanese company CESCO Bioengineering ... human mesenchymal stem cells (hMSC), human foreskin fibroblast cells, and human muscle skeleton cells, noted the firm. ... Bellows boosts cell culture yields. 03-Feb-2004. - Last updated on 19-Jul-2008 at 12:29. GMT ...
LIFE SCIENCES > Cell Biology > Cell Culture > Other cell culture tools *Stem and primary cells. ChiScientificTM provide alls ... IcellTis specialty in adipocytes stem cells, and more.. *Cell lines. Interchim source from major cell collections and small ... COVID-19 RESEARCH , Immunodetection Tools , Biochemistry & Proteomics , Molecular Biology - Genetics , Cell Biology , ... the reagents and toold for the primary cell studies.. Sanguine BioSciences offers High Quality Human Primary Cells and Blood ...
  • Malignant transformation of fallopian tube secretory epithelial cells (FTSECs) is a key contributing occasion to the event of high-grade serous ovarian carcinoma (HGSOC). (
  • Early embryonic growth happens within the oviduct, the place a really perfect microenvironment is offered by the epithelial cells and by the oviductal fluid produced by these cells. (
  • It detaches epithelial cells from tissues in a sheet-like structure and has long been used for cell separation and dispersion for primary culture. (
  • DISPASE is characterized by its high ability to degrade type IV collagen and fibronectin, which constitute the basement membrane, and detaches epithelial cells from tissues in a sheet-like structure. (
  • Transcriptional activation of the proto-oncogene c-jun by asbestos and H2O2 is directly related to increased proliferation and transformation of tracheal epithelial cells. (
  • Organoid cell culture has transformed cell-based assays in drug discovery and basic biology by conferring physiologic relevance to in vitro cell-based biological models. (
  • Dr Clevers' technology allowed, for the first time, the expansion of adult stem cell-derived organoids in genetically stable form and ultimately, the generation of in vitro models of any epithelial disease from any patient. (
  • The first in vitro cell culture techniques were developed more than a century ago, and helped transform biological research. (
  • The application of 3D cell culture is enabling researchers to study processes that would otherwise be difficult to recreate in vitro . (
  • Identifying both common and infrequent reasons why cells may fail to thrive can increase lab efficiency, enhance yield of cellular products, and ensure meaningful, reliable downstream data from in vitro models. (
  • The chemically defined conditions are growth medium suitable for the in vitro cell culture of animal cells in which all of the chemical components are known. (
  • Maintenance of mammalian and bacterial cell lines to support in vitro expression of proteins using DNA and RNA modalities. (
  • It is widely assumed among cell biologists that the fibroblast tendency to readily grow and proliferate in vitro is linked to their function in the body, where they are extremely important for wound healing. (
  • The in vitro study of these tissues has been hampered by the impossibility of achieving stable long-term cultures. (
  • Cell culture is an in vitro tool that aids the understanding of cell biology, tissue morphology and disease mechanisms, as well as playing a role in drug discovery and tissue engineering. (
  • The in vitro culture of primary human endothelial cells is a widely established model system used to investigate several cardiovascular diseases and cancer research applications. (
  • IMSEAR at SEARO: Culture of embryonic mouse brain cells in vitro. (
  • Cobalt Borate Neodecanoate Complex: In Vitro Mammalian Chromosome Aberration Test in Chinese Hamster Ovary Cells. (
  • Cobalt Naphthenate: In Vitro Mammalian Chromosome Aberration Test in Chinese Hamster Ovary Cells. (
  • H-26232: In Vitro Mammalian Chromosome Aberration Test in Chinese Hamster Ovary Cells. (
  • In Vitro Chromosome Aberration Study Performed on the Huntsman Product FAT 40'162/B Reported a Clastogenic Effect on Cultured Chinese Hamster V79 Cells. (
  • In Vitro Chromosome Aberration Test in Chinese Hamster Ovary (CHO-WBL) Cells. (
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (
  • Células que se multiplican in vitro en un medio de cultivo especial para su crecimiento. (
  • Cells propagated in vitro in special media conducive to their growth. (
  • Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (
  • Scientists have for the first time obtained stem cells from livestock that grow under chemically defined conditions, paving the way for manufacturing cell cultured meat and breeding enhanced livestock. (
  • We conclude that lyso-PC can induce growth factor gene expression in cultured endothelial cells and thus may contribute to the migration and proliferation of smooth muscle cells and fibroblasts in various response-to-injury settings in vivo. (
  • These cells are of great importance in the development of bladder cancer since their uncontrolled proliferation causes 90% of these tumors. (
  • Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation. (
  • Used as a replacement for traditional basement membrane extracts in drug delivery, surface-modified medical device, stem cell and tissue engineering scaffold applications - MAPtrix™ delivers superior cell plating efficiency, improved cell morphology and enhanced cell proliferation. (
  • AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. (
  • Generally, VEGF leads to higher endothelial cell proliferation in culture. (
  • In accordance with other reports, cell culture experiments confirmed that TTFields reduce the proliferation of different glioma cell lines in a field strength- and frequency-dependent manner. (
  • Proliferation of these cells is limited up to the time of their senescence, which limits their supply, and they may accumulate chromosomal changes throughex vivoculturing. (
  • In this study, we examined the effects of culturing under ambient (20%) or physiologic (5%) O 2 concentrations on the rate of cell proliferation and on the spontaneous transformation of hMSCs in primary culture and after expansion, because it has been reported that culturing under hypoxic conditions accelerates the propagation of hMSCs. (
  • We found that hypoxic conditions promote cell proliferation more favourably than normoxic conditions. (
  • The effect of the materials on the cultivated fibroblasts was analyzed in terms of short-term and long-term cell growth as well as in viability and cell proliferation assays. (
  • The mechanisms and role of c-jun activation in the aberrant proliferation of cells were studied. (
  • The authors conclude that asbestos and H2O2 activate AP-1-dependent gene transcription and that overexpression of c-jun in HTE cells leads to increased proliferation and cellular transformation. (
  • Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (
  • Previously, organoids were generated from embryonic or induced pluripotent stem cells, or from tumor cells which by necessity are genetically modified and therefore unrepresentative of the patient. (
  • The research " Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species" has been published today in the journal Development and was funded by BBSRC, EU (ERC), MRC and Wellcome Trust. (
  • So the team cultured mouse embryonic fibroblasts and measured the levels of 5hmC over time. (
  • We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the 1-L scale. (
  • We can also see how they resemble and how they differ from tumor cells because tumor cells also proliferate inappropriately and they may share some similarities. (
  • When coated with proteins, cells adhere to and grow on our soft surfaces. (
  • Finally, she has used CRISPR-Cas9 to target genes that can interfere with the quality of the protein product, such as host cell proteins that degrade the product or its attached glycan structures. (
  • In addition to targeting genes and regulatory elements that can impact the expression levels and properties of therapeutic proteins, Dr. Kildegaard's group is using CRISPR tools to answer questions about the basic biology of CHO cells. (
  • The bioreactor is specially designed for high-density culture of mammalian and insect cells, for laboratory production of large quantities of proteins, virus, antibody, cell mass and cell components, according to Metabios. (
  • Since the matrix surface is specially treated, BelloCell can grow both anchorage-dependent and anchorage-independent cells and allow easy harvest of whole cells, cell components or secreted proteins. (
  • This portion of the fluorescence digital image gallery features widefield fluorescence images captured from over 30 cell lines stained with a mixture of synthetic probes, antibodies, and fluorescent proteins. (
  • Our recombinant proteins and functional antibodies precisely guide the fate of target cells, and our Cell-Vive™ reagents enhance cell viability and expansion. (
  • MAPtrix™ technology works by incorporating small individual motifs from ECM proteins into recombinant proteins with a backbone of Mussel Adhesive Protein to attach to the substrate, thereby presenting the ECM motif for cultured cells to attach to. (
  • For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. (
  • Most of the systems used to grow cell lines were designed for the production of vaccines or proteins, with no need to harvest the cells at the end of the process. (
  • Previous reports suggest that electrical forces on cell structure proteins interfered with the chromosome separation during mitosis and induced apoptosis. (
  • A major focus here is on the development of new aptamer-based biosensors for the monitoring of cell culture processes as well as the design, production and integration of a new lab-on-a-chip system that enables continuous, transient gene transfer into host cells for the flexible production of recombinant proteins. (
  • The research group uses 3D-printed microfluidic systems for the flexible production of recombinant proteins by enhanced transient transfection of mammalian cells. (
  • In mammalian cell based models of both polyglutamine and polyalanine diseases, the mutant proteins are much more prone to aggregate formation than their wild-type counterparts and cause significantly more cell death. (
  • In the first 30 min of treatment, the response was similar to the cell culture: there was a decrease in metabolites of the TCA cycle and amino acid biosynthesis and the transcriptomic response was dominated by up-regulation of DNA regulatory proteins. (
  • Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. (
  • In this study, we have found that lyso-PC increased steady state mRNA levels for two smooth muscle/fibroblast-directed growth factors, the A and B chains of PDGF and heparin-binding EGF-like protein (HB-EGF), in cultured human endothelial cells. (
  • Lyso-PC did not upregulate the expression of certain other inducible endothelial genes, including E-selectin, IL-8, or monocyte chemoattractant protein-1 in the same cells, in contrast to the coordinate pattern of activation typically observed with other stimuli, such as TNF alpha, bacterial endotoxin, or PMA. (
  • The cells exhibit endothelial morphology and are widely used in studies relating to hypertension, atherosclerosis, and coronary heart disease. (
  • Our comprehensive endothelial cell culture portfolio consists of 12 different types of large vessel and microvascular human endothelial cells with optimized growth media for each cell type. (
  • PromoCell large vessel endothelial cells are available from the umbilical vein and artery, the aorta, the coronary artery, the pulmonary artery, and the saphenous vein. (
  • Our microvascular endothelial cells are available from dermal, lung, cardiac and uterine tissues. (
  • Endothelial cells from diseased donors (e.g. diabetes, myopathies) are available upon request. (
  • At PromoCell we have developed optimized growth media for both large vessel and microvascular endothelial cell types. (
  • In addition, we offer media formulations with and without Endothelial Cell Growth Supplement (ECGS), a bovine hypothalamic extract traditionally used for cultivating endothelial cells without added VEGF. (
  • HMEC-1 is an endothelial-like cell that was isolated from the endothelium of the foreskin of a male patient. (
  • Though this technique is highly accessible, successful propagation of cells for stock expansion or modeling experiments can be plagued by contamination or other conditions that negatively impact cell viability. (
  • Studies in association with the Institute of Chemistry and Biotechnology of the Zurich University of Applied Sciences in Winterthur have shown that various cell types grown to near confluency in Corning RoboFlask vessels showed no statistical differences in cell counts and cell viability when harvested either manually or by the Freedom EVO equipped with the Flask Flipper (data available from Tecan). (
  • Each produced cell lot is tested for cell morphology, adherence rate, cell viability, flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake. (
  • Furthermore, the group deals with biocompatibility studies to ensure an ideal environment for cell viability and recombinant protein production. (
  • The researchers report that the paper's porosity and 3D cellulose fiber network offer a highly protective environment for the cells to retain their viability and content during preservation. (
  • As a result, after their freeze, thawed cells are efficiently released from paper with viability rates comparable to conventional methods. (
  • Unlike other scaffold-based cryopreservation strategies such as fiber meshes and nanofiber sheets, where the substrates must be repeatedly engineered for their use in cell cryopreservation, this paper-based method is solely based on the ready-to-use papers where cells are preserved with no significant effect on their viability and metabolic activity," said Roaa Alnemari, a former Research Assistant with Qasaimeh's lab. (
  • Cell growth and viability were assessed after 7 and 10 days. (
  • 001). Cell viability in all the groups was over 80%, except in group IV at day 7. (
  • Similarly, transformed cells grown on plastic have modified their gene expression to adapt to tissue culture conditions. (
  • Here we utilize CRISPR/Cas9 technology for targeted gene modification of four of the most commonly mutated colorectal cancer genes (APC, P53 (also known as TP53), KRAS and SMAD4) in cultured human intestinal stem cells. (
  • These novel cell lines can differentiate into multiple cell types, they can be genetically manipulated using Crispr/Cas9 gene editing tool and can be used as donors for nuclear transfer. (
  • Using CRISPR, her team generates pools of cells such that each pool has a different gene knockout. (
  • Meehan's group found that Tet gene expression declined as cells spent more time in culture. (
  • Nuclear runoff assays documented an increased transcriptional rate for the HB-EGF gene in lyso-PC-treated cells. (
  • Specific applications include protein and virus production, monoclonal antibody production, making whole cells and cell components for binding assays, gene and cell therapy, proteome research and tissue engineering. (
  • The team also showed that the acquisition of functional properties is dependent on Notch, a gene that in bladder cancer functions as a tumor suppressor: Notch activation causes cells to differentiate, which is a normal function of the cell that serves as a protection mechanism against tumor growth. (
  • For example, experiments to look at cell appearance, gene expression and cell behaviour. (
  • Main objective in this project has been to isolate and characterize the different embryogenic cell types in Brassica napus microspore culture using a set of fluorescently-tagged gene reporters. (
  • The PPL scientists introduced a gene in the chromosomes of livestock cells in culture. (
  • The candidate will be responsible for maintaining cells for plasmid production and to drive efforts in developing novel cell-based assays to evaluate RNA modalities using state-of-the-art technologies and equipment. (
  • That being said, if you have extremely sensitive cells and/or assays, you may choose to filter sterilize your media prior to use. (
  • HUB's is based on pioneering work by Professor Hans Clevers, who in 2009 first described methods for growing and expanding human adult stem cell-derived human epithelial organoids. (
  • In addition HUB generated models for other diseases such as Inflammatory Bowel Disease (IBD), Chronic Obstructive Pulmonary Disease (COPD) and genetic diseases such as cystic fibrosis (CF). The company has also introduced adult stem cell-derived kidney organoids, and continues to investigate the molecular mechanisms of tissue development and cancer using organoids generated from adult Lgr5 stem cells. (
  • Hundreds of companies in the United States are advertising dozens of unapproved stem cell therapies for direct-to-consumer (DTC) sale, according to a new study. (
  • A lax regulatory environment has permitted a "cowboy culture" to flourish, the authors argue in an article published online June 30 and in the August 4 issue of Cell Stem Cell . (
  • In a news release about the study, the authors say their curiosity was piqued when Dr Knoepfler started receiving enquiries about US-based stem cell businesses in a blog he maintains called The Niche . (
  • For example, current discussions of stem cell therapies usually concern "stem cell tourism" to centers outside the United States and rarely acknowledge the US businesses engaged in DTC advertising. (
  • After using phrases such as "stem cell therapy" and "stem cell treatment" to identify the companies, they visited each company's website to learn more about their claims and the stem cell products they used. (
  • The search uncovered 351 businesses marketing stem cell treatments at 570 clinics. (
  • The findings provide "clear grounds for concern" that some of these companies may not be complying with federal regulations covering stem cell therapies, with related questions about the information provided to prospective patients, Dr Turner and Dr Knoepfler warn. (
  • When we start moving into 3D, we see that brain cells just do better," explains stem cell biologist, Alysson Muotri, of the University of California, San Diego. (
  • Both mouse and human intestinal stem cells can be cultured in medium containing the stem-cell-niche factors WNT, R-spondin, epidermal growth factor (EGF) and noggin over long time periods as epithelial organoids that remain genetically and phenotypically stable. (
  • Quadruple mutants grow independently of all stem-cell-niche factors and tolerate the presence of the P53 stabilizer nutlin-3. (
  • Researchers from the University of Nottingham's School of Biosciences, together with colleagues at the Universities of Cambridge, Exeter, Tokyo and Meiji (Japan) have developed stem cell lines from pigs, sheep and cattle embryos grown without the need for serum, feeder cells or antibiotics. (
  • The researchers went a step further and, thanks to single-cell RNA sequencing, were able to identify the nature of the urothelial stem cell population of these organoids that can proliferate and give rise to cancer. (
  • Scaling up stem-cell cultures requires careful consideration of the bioreactor design. (
  • There are two main criteria that must be considered when selecting a system for stem-cell bioprocessing and recovery. (
  • Stem-cell culture and differentiation are sensitive to many different factors. (
  • In this model, the bioreactor enables stem-cell expansion in a closed system with fewer operations needed. (
  • Banking on Your Future, a guest commentary by Dr. Peter Hollands Hardly a day goes by without a new revelation in stem cell technology. (
  • Whatever the present and future applications of stem cell technology may be it is becoming increasingly obvious that stem cell technology is big business in particular in the field of private banking of stem cells . (
  • While the neurosphere assay is a valuable procedure from many biological perspectives, the user should be aware that there are particular relationships between the definitive neural stem cell and the production of neurospheres in culture that the can lead to shortcomings 7 . (
  • Comment: Patients should not take antiretroviral medications for at least 1 month before initiating medications for stem cell mobilization, for the duration of the medications? (
  • A new report from the U.S. Government Accountability Office (GAO) says federal food safety agencies "could strengthen existing efforts to prepare for oversight of cell-cultured meat. (
  • As an expanding number of startups pursue the commercialization of cultivated meat, a select few are targeting the hybrid approach - blending small quantities of lab-grown cells into primarily plant-based products. (
  • This can accelerate the pace of genetic selection of livestock and cultured meat to improve productivity and creation of healthier foods. (
  • It will be considered a win-at least initially-by most of the fledging food technology startups that are looking to bring cell-cultured meat to market. (
  • Clean meat is a meat product grown from animal cells that does not require animal slaughter. (
  • The agency meeting is designed to give cell-cultured meat companies the opportunity to publicly share with FDA officials their cases for why their products should have a place in American grocery stores and restaurants. (
  • A joint letter sent on Monday, 8 March, by the National Fisheries Institute (NFI) and the Alliance for Meat, Poultry, and Seafood Innovation (AMPS Innovation) has called on the U.S. Food and Drug Administration (FDA) to ensure proper labeling of cell-cultured seafood. (
  • AMPS Innovation is an industry coalition of companies growing cell-based meat, poultry, and seafood, and has previously sent a joint letter with the North American Meat Institute calling for similar labeling. (
  • In addition, the labels for cell-cultured seafood should be consistent and uniform within the category, and also consistent with the meat and poultry categories, to reduce confusion. (
  • Mycoplasma contamination is indeed a key factor to evaluate during a cell culture projects, as it can influence cell behavior, morphology and function. (
  • Some cells remained isolated but with altered morphology ( Figure 1F ). (
  • COS-7 cells exhibit fibroblast morphology and are often utilized in transfection experiments. (
  • Aim: The aim of the present study was to assess the toxicity of different chemically activated GICs in fibroblast cell cultures post-agglutination (PA) and post-setting reaction (PS). (
  • The researchers tried again with murine CD4+ T cells and found the same thing-the marks vanished. (
  • Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (
  • The growth of this market is majorly driven by the growing awareness about the benefits of cell-based vaccines, increasing demand for monoclonal antibodies (mAbs), funding for cell-based research, growing preference for single-use technologies, the launch of advanced cell culture products, and the growing focus on personalized medicine. (
  • The system was validated with a random panel of 45 whole-cell culture broths containing recombinant antibodies in the early profiling phase. (
  • C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (
  • Incubation in tissue culture is 40-72 hours, depending on the cell type and specific biovar. (
  • Stem cells and their progeny, from all ages of the CNS, can be stimulated to proliferate when they are exposed to growth factors in tissue culture 1 . (
  • 11- 13 A causal role for aggregation in cell death in tissue culture models of OPMD is supported by complementary data from our lab and Rouleau's group. (
  • Obviously, these studies are done using mouse embryo fibroblasts and not human cancer cells, but the changes in 5hmC. (
  • We demonstrate 3D cell culture of HepG2 hepatocytes under continuous perfusion, a rifampicin toxicity assay and co-culture with fibroblasts. (
  • Results of search for 'su:{Cells, Cultured. (
  • In Genome Biology today (February 3), researchers reported yet another gap between culture and critter: an epigenetic mark, 5-hydroxymethylcytosine (5hmC), all but disappears from certain mouse cells a few days after they're transferred to a dish. (
  • This paper adds substantial fuel to the fire of concern about using cultured cells to study phenotypes associated with cancer in vivo (such as drug resistance)," Michael Gottesman , chief of the Lab of Cell Biology at the National Cancer Institute's Center for Cancer Research, told The Scientist in an e-mail. (
  • Rapid reprogramming of epigenetic and transcriptional profiles in mammalian culture systems," Genome Biology , doi:10.1186/s13059-014-0576-y, 2015. (
  • The candidate is expected to have a strong background in cell and molecular biology with experience in bacterial and mammalian cell culture. (
  • On the other hand, the high cost of cell biology research is restraining the growth of this market. (
  • The research group "Technical Biology", headed by Prof. Dr. Janina Bahnemann, focuses on the fabrication and integration of 3D-printed microfluidic systems and the development of innovative biosensors for applications in the field of cell culture engineering and point-of-care diagnostics. (
  • The U.S. Department of Agriculture (USDA) and the FDA entered a formal agreement on 7 March, 2019, outlining each agency's role in the oversight of cell-cultured technology. (
  • The multifunctional polymer coating adhered strongly to our soft substrates and enabled cell adhesion, growth and differentiation. (
  • The neurosphere assay has proven to be an excellent technique to isolate neural stem cells and progenitor cells to investigate the differentiation and potential of cell lineages. (
  • Bone marrow derived human mesenchymal stem cells (hMSCs) have attracted great interest from both bench and clinical researchers because of their pluripotency and ease of expansionex vivo. (
  • When provided with a suitable growth environment, including appropriate cultureware, growth factors, extracellular matrix, nutrients, and culture media, organ-derived progenitor cells harvested from patients grow and assemble into three-dimensional structures - organoids - which incorporate all cell types normally found in the original tissue, and allow physical and chemical interactions between and among cells. (
  • By providing greater physiologic relevance and a species- or patient-specific test platform, organoids overcome many limitations of conventional 2D cultures and even live-animal disease models. (
  • Organoids arise from organ-derived adult pluripotent stem cells, organ stem cells, or cancer stem cells which possess the innate capacity to expand and differentiate into multiple cell types. (
  • When cultured under identical HUB protocols adult progenitor cells give rise to organoids with exactly the same cells in the same proportions, physical configuration, and genetics, every time, and with broad expansion capabilities. (
  • There is a variety of 3D culture technologies - including spheroids, organoids, scaffolds and hydrogels - that differ in their principles and protocols, yet they all aim to recapitulate the morphological, functional and microenvironmental features of human tissues and organs. (
  • Derived from primary tissue or stem cells, 3D organoids are capable of self-renewal, self-organization, and exhibit organ functionality. (
  • Fuelled by the excitement around cancer immunotherapies, scientists are creating 3D organoids made from a co-culture of tumour and immune cells, to study their interactions 2 . (
  • Mutant organoids can be selected by removing individual growth factors from the culture medium. (
  • Subsequently, they characterized the organoids by sequencing the RNA of individual cells and identified a small population of stem cells -proliferative cells- that gives rise to the organoids and that could not be studied until now. (
  • With support from the Gebert Rüf Stiftung, we could identify and design innovative and potent multifunctional polymers that are cost-effective and can be used as stable coating agent for our in vivo-like cell culture substrates. (
  • Cells in suspension mimic in vivo conditions as singlets. (
  • Incorporating mix-and-match genetically incorporated bioactive peptides - MAPtrix™ provides an in-vivo-like surface structure, that mimics the native extracellular environment, enabling the maintenance of cells under serum and feeder-free conditions. (
  • 11, 15 Members of the HSP70 and HSP40 family members are recruited to polyQ inclusions in vivo and in cell models. (
  • The following article originally appeared on September 8, 2020 in Cell Science from Technology Networks here . (
  • The following article originally appeared on June 15, 2020 in The Cell Culture Dish here . (
  • The company has launched 4 products in the cell culture market since 2020. (
  • Abu Dhabi, UAE - 29 January 2020: Researchers from the Division of Engineering at NYU Abu Dhabi (NYUAD) have developed a new technique that utilizes filter paper to cryopreserve human cells, offering scientists an efficient alternative to conventional, long-term cryopreservation methods. (
  • Some companies marketed a combination of therapies, usually a mixture of autologous cells from adipose tissue and bone marrow. (
  • We investigated whether endoplasmic reticulum (ER) stress, which is characteristic of obese adipose tissue, regulates resistin expression in cultured mouse adipocytes. (
  • Under HUB's commercial development, organoid technology also provides standardization and consistency which is difficult to match, especially with primary cell cultures. (
  • 3D cell cultures have been grown from various cells, including transformed cell lines and primary cells. (
  • Conventional primary cell culture methods expand only for several generations if at all, thus limiting their suitability to biobanking or large-scale manufacturing. (
  • Furthermore, conventional 3D primary-cell culture methods lack consistency and suffer from poor manufacturability conferring a "one-off" character to studies employing them. (
  • Moreover, expression analysis experiments on aggregated primary cell cultures are unreliable since the analysis must occur within the limited timeframe before the aggregate loses integrity. (
  • ChiScientific TM provide alls the reagents and toold for the primary cell studies. (
  • Furthermore, smoothed hazard-function modelling of chromosomal aberrations showed increased hazard after the fourth passage under both sets of culture conditions, and showed a tendency to increase the detection rate of primary karyotypic abnormalities among donors aged 60 years and over. (
  • The next day, S. zooepidemicus was cultured from the CSF and four of the four blood culture bottles, leading to a primary diagnosis of meningitis and sepsis. (
  • Control cell lines were injected with filtered hepatopancreas extracts from healthy shrimp. (
  • Fuse, H 1995, ' Transformation of Dimethyl Sulfide and Related Compounds by Cultures and Cell Extracts of Marine Phyto plankton ', Bioscience,Biotechnology, and Biochemistry , vol. 59, pp. 1773-1775. (
  • It is inescapable to understand biological processes, test potential drugs, assess toxicity of chemical compounds, and multiply cells for cell therapy. (
  • Such processes were highlighted at two recent conferences: Optimizing Cell Culture Technology (part of the Ninth Annual Bioprocessing Summit) and Bioprocess International West. (
  • The module eliminates manually tedious cell culturing processes by automating the harvest, passage, splitting and seeding of adherent cell cultures. (
  • Node Smith, ND Silicone molecules from breast implants can initiate processes in human cells that lead to cell death. (
  • Whether it is to improve processes in your laboratory, extend the life of your equipment or minimize the contamination risk, we have gathered the following top tips for your cell culture equipment. (
  • Although the production processes are similar, a difference is that the virus strains included in the vaccine are grown in animal cells of mammalian origin instead of in eggs. (
  • Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (
  • The COS-7 cell line is a line developed from the standard CV-1 African green monkey kidney line by transforming the normal cells with an origin defective mutant of simian virus 40 that codes for the wild-type large tumor antigen. (
  • Many researchers are moving away from using cells grown in monolayers, which do not represent their growth in the body. (
  • When cells seem viable but fail to expand, learn what you can do to improve culture conditions for optimal growth before heading back to the liquid nitrogen. (
  • COS-7 cells fully permit the lytic growth of SV40 and the replication of populations of SV40 mutants with deletions in the early region. (
  • Unlike the original CHO line, CHO-K1 cells require proline in the medium for growth in culture. (
  • CO 2 incubators provide optimal conditions for cell growth, controlling temperature, humidity and CO 2 levels (O 2 control optional). (
  • The aim of the study was to analyze molecular mechanisms involved in FSH and basic fibroblast growth factor (bFGF) regulation of lactate production in rat Sertoli cells, and showed that bFGF increases phosphorylated PDC levels and that FSH-stimulated lactateProduction is partially inhibited in the presence of a PDK inhibitor. (
  • With its strong product portfolio, continuous research and development activities, and broad geographic reach, Thermo Fisher Scientific is expected to witness significant growth in the global cell culture market. (
  • These pipetting solutions are ideal for applications ranging from large volume, traditional 2D methods - for cell growth and the production of cell-derived biomolecules - to smaller volume scaffold-based (hydrogel) and scaffold-free (spheroid) 3D techniques, as well as drug toxicity testing and pharmacokinetic/pharmacodynamic studies. (
  • The overall market for regenerative medicine has been predicted to reach as much as $20 billion by 2025, according to research from Scientia Advisors, and if this growth is to come about, there must be reliable, efficient methods for scaling up the production of the stem cells that are used in many of these therapies (1). (
  • The growth, integrity, and quality of the cells must be preserved and controlled, and it must be possible to harvest and recover the cells smoothly. (
  • Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. (
  • Tumour-treating fields (TTFields) use alternating electric fields which interfere with dividing cells, thereby reducing tumour growth. (
  • In this study, a novel strategy is proposed, which leverages saturation and sigmoidal relationships, along with an underlying material balance framework, for the automated assembly of hybrid dynamic models of cell growth. (
  • No growth of bacteria, viruses, or fungi in the culture is normal. (
  • The groups having received the materials following the setting reaction had a lower number and percentage of viable cells in comparison to the control, but the materials allowed cell growth throughout the experiment. (
  • Conclusion: All glass ionomer cements tested were toxic prior to the setting reaction.However after setting reaction, allowed cell growth. (
  • The effect of overexpression of c-jun on cell growth rates was studied by determining cell numbers of RSV-O and RSV-jun 24hr, 48hr, and 72hr after low density plating. (
  • InvivoGen provides a wide range of high-quality reagents for tissue and cell culture applications , including mycoplasma detection , prevention and elimination, plus various antimicrobial agents , selective antibiotics , reporter detection kits , the transfection reagent LyoVec™ , the transfection enhancer NATE™ , and an endotoxin detection kit . (
  • Appropriate equipment, qualified reagents, and expert protocols are key to resolving unexpected cell culture outcomes . (
  • We also provide reagents produced under Current Good Manufacturing Practices (cGMP) to support cell manufacturing applications. (
  • In order to recapitulate the ocular fundus functions, neural supporting cells such as retinal pigment epithelial (RPE) cells were cultured within a three-dimensional microfluidic device, and cell responses at the tissue level to changes in the microenvironment were analyzed. (
  • Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (
  • Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an. (
  • However, traditional 2D cultures grown on flat surfaces don't represent how cells live and function in the body where they are surrounded by other cells. (
  • Further experiments have been carried out to characterize the behavior of cells grown on our innovative culture substrates. (
  • The plates are similar to those on which stem cells are grown on multiplate stacks in the laboratory. (
  • Why Use Antibiotics in Cell Culture? (
  • Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents. (
  • In addition, methods for preventing and rescuing contaminated cultures and the use of antibiotics were covered. (
  • Levofloxacin, cefuroxime and azithromycin were the most common antibiotics prescribed for the groups with positive sputum culture and no sputum collection. (
  • And drug discovery researchers are using 3D cell cultures to screen for new immuno-oncology agents. (
  • In fact, the researchers found that adding Tet cofactor vitamin C to the culture rescued some of the loss of 5hmC. (
  • The study points to how researchers might further improve culture conditions, Meehan said. (
  • During the last 8 years, the Cell Culture and Tissue Training Course has become an important tool for students, researchers and all the professionals in the field of biomedical sciences, allowing the implementation of new approaches on their research. (
  • This makes it very difficult for researchers to work with these cells and, consequently, to make progress in the study of diseases resulting from their incorrect functioning. (
  • Our work enables researchers who are investigating other pathologies to have stable cell populations in large numbers to study these diseases. (
  • Experience a countdown through 10 experiments that researchers do using tonsils and the cells they culture from them. (
  • These findings provide a promising prospect for industrial cell banks and researchers engaged in tissue engineering, drug testing, and other fields that rely on long-term, successful preservation of cells. (
  • For example, to enhance the performance of the CHO cell factories, she has used CRISPR-Cas9 to knockout pro-apoptotic genes, which tend to increase in expression under the higher stress conditions of cell culture and activate programmed cell death pathways. (
  • Also, she has used CRISPR tools to overexpress genes that help increase the productivity of CHO cells in culture. (
  • The book is an unintended eye-opener to the relationship between genes and culture. (
  • Kris Wronski, Cell Culture Scientist at Thermo Fisher Scientific, shares his experience of working in a busy cell culture lab and key factors to minimize the risk of contamination. (
  • View our cell culture poster with all essential workflow steps and products from Thermo Fisher Scientific to advance your research. (
  • The global cell culture market is a consolidated market, with Thermo Fisher Scientific, Inc. (US), Merck KGaA (Germany), Danaher Corporation (US), Sartorius AG (Germany) together accounting for ~60 65% of the global market. (
  • Thermo Fisher Scientific (US) offers cell culture products such as consumables, instruments, and equipment through three segments Life Sciences Solutions, Specialty Diagnostics, and Laboratory Products & Services. (
  • From 2019 to 2021, Thermo Fisher Scientific launched 8 cell culture products, including cell culture media, cultureware, and equipment. (
  • We have previously reported that lyso-PC can activate cultured endothelia, resulting in the selective upregulation of adhesion molecules, such as vascular cell adhesion molecule-1 and intercellular adhesion molecule-1. (
  • Their functionality is determined by the interplay between different cell types, their secondary architecture, vascular system and gradients of signaling molecules and metabolites . (
  • This project supported by the Gebert Rüf Stiftung is of high significance since design of our novel cell-adhesive and soft surfaces will greatly advance current cell culture methods. (
  • Other topic areas which will be featured at Cell Culture 2012 are the challenges involved with preventing cell line cross contamination and the methods used to mitigate these risks, as well as novel methods in the use of 3D cell cultures. (
  • IcellTis specialty in adipocytes stem cells, and more. (
  • Abstract: In this work, the design and execution of a mechanistic metabolic model is presented that is capable of simulating extracellular metabolite concentration profiles, particularly cell density and antibody titer, throughout the course of a recombinant protein producing CHO fed-batch culture. (
  • Biochemically defined and animal-free - Mussel Adhesive Protein based matrix (MAPtrix™) from AMSBIO provides a highly defined and controllable recombinant extracellular matrix (ECM) for cell culture, which can be tailored to the specific needs of particular cell types. (
  • The surface composition and geometry affect the cells' connections and the extracellular matrix. (
  • The common practice of sharing cells has led to well-published evidence of cross-contamination of cell stocks whose identity was previously unquestioned. (
  • Contamination with aggressive cell lines: Isoenzyme analysis has demonstrated that many cell lines share a rare enzyme isoform with HeLa cells. (
  • Be informed about common causes of cell line cross-contamination and how to protect your work from its consequences. (
  • When cells die in culture, contamination with microorganisms must first be ruled out. (
  • If it's not contamination, particulates in culture often have an inorganic explanation. (
  • We concluded our Ask the Expert session on how to minimize contamination risk and protect your cultures. (
  • Laboratory and cell culture contamination is a serious risk for research labs, process development labs and biomanufacturing. (
  • Contamination can compromise cell culture and experiment results, thus wasting valuable resources conducting experiments that ultimately aren't accurate. (
  • Contamination can be introduced through human interaction, cross-contamination with an already infected culture, poor lab techniques, and contaminated raw materials, just to name a few. (
  • In addition, contamination isn't always easy to detect, which means that contamination can spread to multiple cultures before the problem is identified. (
  • With contamination lurking everywhere, it may feel daunting to think about protecting precious cultures. (
  • You can minimize the risk of contamination with a combination of good cell culture practices, aseptic techniques, and the advanced capabilities of our lab equipment. (
  • Our outstanding contamination control technologies are designed to provide effective, convenient protection from microorganisms that threaten your valuable cultures. (
  • Cleanability, filtration, UV light, copper surfaces and more features help minimize contamination in your lab and protect your cells. (
  • Microbial contamination of cell culture is a major concern for cell biologists in research laboratories as well as in biotechnological industries. (
  • Merck KGaA (Germany) provides cell culture separation and purification tools through its Research Solutions subsegment, single-use manufacturing and filtration products through its Process Solutions subsegment, and lab water and microbiology solutions through its Applied Solutions subsegment. (
  • Changing the niche environment in which they grow by altering the surface can have a large effect on the cell behavior. (
  • The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (
  • When I first worked in this field nearly 30 years ago there was little interest in stem cells beyond the highly specialized experts working on bone marrow transplantation. (
  • Bone marrow culture is an examination of the soft, fatty tissue found inside certain bones. (
  • The bone marrow tissue produces blood cells. (
  • The authors of the following chapters discuss the culturing of specific cell types. (
  • They all lack vasculature (although next-generation models are under development), some are missing crucial cell types, and many replicate only the earliest stages of organ development. (
  • Within a patient's tumour, there are lots of other cell types, such as immune cells and stromal cells, so you can start adding these and look at how they interact with the cancer cells," explains Natrajan. (
  • Investigation of biological mechanisms in urothelial tissue -one of the three types of tissue that make up the urinary system- has always been very limited due to the lack of technologies that allow working with stable and long-term populations of the cells that compose it. (
  • All our cell types are sourced from healthy, consented donors. (
  • It is, therefore, essential that we know the final fate of the different cell types in culture to be able to link these fates to specific signalling pathways and to understand their role in totipotency. (
  • 1. Define and collect the different embryogenic cell types present in microspore culture using GFP-based reporters, time-lapse imaging, and cell sorting. (
  • Time-lapse imaging of the embryo-expressed GFP reporter lines was used to determine the fate of the different embryogenic cell types in culture. (
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (
  • In the present study, we found that resveratrol dramatically suppressed the replication of vaccinia virus (VACV), the prototypic member of poxviruses, in various cell types. (
  • Dynamic Model of Chinese Hamster Ovary Cell Metabolism in Fed-Batch Culture. (
  • But, because of its multiple effects on the cell metabolism, it may also interfere with certain experimental setups. (
  • This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. (
  • Now, scientists from the Epithelial Carcinogenesis Group led by Paco Real at the Spanish National Cancer Research Centre (CNIO) used innovative organoid technology to achieve stable long-term cultures of mouse urothelial cells. (
  • Organoid technology allows cell cultures to grow in a three-dimensional matrix called Matrigel. (
  • In addition, DISPASE, which is characterized by lower cytotoxicity and gentler cell dispersion than trypsin, collagenase, and other proteases, is also used in regenerative medicine such as ES/iPS cells. (
  • The cells are also often employed in transfection experiments with simian virus 40 ( SV40 ) and recombinant plasmid vectors. (
  • The use of infected cell lines can lead to unreliable experiments and unsafe biologicals and biopharmaceutical drugs, and is costly in time and materials. (
  • Our substrates retain all advantages and the easy handling of conventional culture ware. (
  • The coating does not interfere with conventional biological techniques to retrieve cells for biochemical or molecular analysis. (
  • The product's label should be clear, non-misleading, and communicate what the product is and how it is used in line with FDA regulatory requirements, labeling should follow current allergenicity and nutrition rules, should not disparage either other cell-cultured or conventional seafood products, and should differentiate itself from conventional products through some form of qualifier that modifies the conventional name of the product. (
  • According to an FDA news release, cell culture technology represents an alternative to conventional egg-based influenza vaccine production. (
  • The conventional preservation approach places cells in small vials where their metabolic activities are "frozen" at extremely low temperatures (-80 ºC or below, typically -196 ºC for long-term storage). (
  • As NYUAD Assistant Professor of Mechanical and Biomedical Engineering Mohammad A. Qasaimeh and colleagues explain in the study, "Paper-based Cell Cryopreservation," published in the journal Advanced Biosystems , a conventional filter paper made of cellulose fibers offers a simple and robust alternative, allowing for easy loading and efficient freezing of cells. (
  • Cell culture media and incubation conditions provide the ideal environment for cells, as well as bacterial, fungal, and viral contaminants. (
  • However, because TSV could potentially represent a public health threat, we explored whether this viral agent might be capable of infecting cultured mammalian cells. (
  • RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (
  • The cells have also tested positive for bovine diarrhea virus, an important bovine viral pathogen. (
  • PCR) or viral culture. (
  • Subsequent 16hr incubation with asbestos decreased the percentage of BrdUrd positive transfected cells relative to untreated RSV-O control cells. (
  • By contrast, 3D cultures can model different oxygen-nutrient gradients so that cells in the centre of a spheroid experience hypoxia, explains breast cancer researcher, Rachael Natrajan of the Institute of Cancer Research, London. (
  • More complex 3D models that can mimic the tumour microenvironment are also facilitating research into interactions between cells. (
  • Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential process in life science research labs. (
  • Following up on their observation that 5hmC levels were low to undetectable in various human cancer cell lines, Richard Meehan at the Medical Research Council's Human Genetics Unit in Edinburgh and his colleagues decided to figure out what might contribute to this phenomenon. (
  • Traditionally, research on the replication of shrimp viruses has been based on the use of cultured fish cellular lines ( 6 ). (
  • The cells, which are heavily utilized in biomedical research laboratories around the world, exhibit contact inhibited motility and generally tend to form confluent monolayers. (
  • Activating and expanding target cells are critical early steps to produce a sufficient number of cells for research or therapeutics. (
  • Recent studies estimate misidentification may affect up to one third of all cell lines in use. (
  • Cell lines should only be obtained from reputable cell repositories such as ECACC, the European Collection of Authenticated Cell Cultures at Public Health England. (
  • The cell lines we developed are a step change from previous models as they have the unique ability to permanently grow to make muscle and fat. (
  • Suppliers of cell lines and raw material should conduct extensive testing before releasing a product, and provide documentation listing what tests were done, and indicate the level of sterility. (
  • Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (
  • Fresh cell lines were inoculated with the lysate, incubated, and processed in a similar way. (
  • Similar to other fibroblast lines, A.P. Mongoose cells are relatively easy to grow in culture dishes. (
  • DISPASE is stable at 37°C, the common cell culture temperature, and can be applied to a medium for suspension culture of some cell lines. (
  • Using FACS, we could separate 100% GFP-positive and 100% GFP-negative cell populations in the GRP:GFP and LEC1:GFP lines until day 6 of culture (Figure 3). (
  • Samples were collected from microspore culture with and without TSA, at day 0, 2, 4 and six days after stress treatment, using three replicates from two different Brassica napus lines. (
  • In addition, they are unsuitable for large numbers of patients (eg, in the emergency department [ED]). Nevertheless, in certain clinical situations, cultures are mandatory. (
  • In conjunction with the NIHR BTRU in the Manufacture and Clinical Assessment of Cultured Red Cells this work is now being taken to the clinical evaluation phase. (
  • However, problems appear when the product moves into the later stages of clinical trials and larger numbers of cells are required. (
  • All sorts of claims are made for the current and future clinical applications of these cells based sometimes on fact and sometimes on wishful thinking. (
  • Suspected and confirmed SARS-CoV-2 positive clinical specimens, cultures, or isolates should be packed and shipped as UN 3373 Biological Substance, Category B. (