Natural History: A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)Leeches: Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Otolaryngology: A surgical specialty concerned with the study and treatment of disorders of the ear, nose, and throat.Forensic Genetics: The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.New Zealand: A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)Plant Cells: Basic functional unit of plants.Vanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Taxus: Genus of coniferous yew trees or shrubs, several species of which have medicinal uses. Notable is the Pacific yew, Taxus brevifolia, which is used to make the anti-neoplastic drug taxol (PACLITAXEL).Petroselinum: A plant genus of the family APIACEAE used for flavoring food.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Cell SeparationChemistry, Analytic: The branch of chemistry dealing with detection (qualitative) and determination (quantitative) of substances. (Grant & Hackh's Chemical Dictionary, 5th ed)Beer: An alcoholic beverage usually made from malted cereal grain (as barley), flavored with hops, and brewed by slow fermentation.Chemistry Techniques, Analytical: Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.IndianaQuackery: The fraudulent misrepresentation of the diagnosis and treatment of disease.Hospices: Facilities or services which are especially devoted to providing palliative and supportive care to the patient with a terminal illness and to the patient's family.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Existentialism: Philosophy based on the analysis of the individual's existence in the world which holds that human existence cannot be completely described in scientific terms. Existentialism also stresses the freedom and responsibility of the individual as well as the uniqueness of religious and ethical experiences and the analysis of subjective phenomena such as anxiety, guilt, and suffering. (APA, Thesaurus of Psychological Index Terms, 8th ed.)Holistic Health: Health as viewed from the perspective that humans and other organisms function as complete, integrated units rather than as aggregates of separate parts.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Great BritainComputer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.PaperTime Factors: Elements of limited time intervals, contributing to particular results or situations.Induced Pluripotent Stem Cells: Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.Germ Cells: The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Carcinoma, Embryonal: A highly malignant, primitive form of carcinoma, probably of germinal cell or teratomatous derivation, usually arising in a gonad and rarely in other sites. It is rare in the female ovary, but in the male it accounts for 20% of all testicular tumors. (From Dorland, 27th ed & Holland et al., Cancer Medicine, 3d ed, p1595)Nuclear Reprogramming: The process that reverts CELL NUCLEI of fully differentiated somatic cells to a pluripotent or totipotent state. This process can be achieved to a certain extent by NUCLEAR TRANSFER TECHNIQUES, such as fusing somatic cell nuclei with enucleated pluripotent embryonic stem cells or enucleated totipotent oocytes. GENE EXPRESSION PROFILING of the fused hybrid cells is used to determine the degree of reprogramming. Dramatic results of nuclear reprogramming include the generation of cloned mammals, such as Dolly the sheep in 1997.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Hepatitis, Infectious Canine: A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Neural Cell Adhesion Molecules: Cell adhesion molecule involved in a diverse range of contact-mediated interactions among neurons, astrocytes, oligodendrocytes, and myotubes. It is widely but transiently expressed in many tissues early in embryogenesis. Four main isoforms exist, including CD56; (ANTIGENS, CD56); but there are many other variants resulting from alternative splicing and post-translational modifications. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, pp115-119)Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)Polycystic Kidney, Autosomal Dominant: Kidney disorders with autosomal dominant inheritance and characterized by multiple CYSTS in both KIDNEYS with progressive deterioration of renal function.Renin: A highly specific (Leu-Leu) endopeptidase that generates ANGIOTENSIN I from its precursor ANGIOTENSINOGEN, leading to a cascade of reactions which elevate BLOOD PRESSURE and increase sodium retention by the kidney in the RENIN-ANGIOTENSIN SYSTEM. The enzyme was formerly listed as EC 188.8.131.52.Kidney Tubules, Proximal: The renal tubule portion that extends from the BOWMAN CAPSULE in the KIDNEY CORTEX into the KIDNEY MEDULLA. The proximal tubule consists of a convoluted proximal segment in the cortex, and a distal straight segment descending into the medulla where it forms the U-shaped LOOP OF HENLE.TRPP Cation Channels: A subgroup of TRP cation channels that are widely expressed in various cell types. Defects are associated with POLYCYSTIC KIDNEY DISEASES.Polycystic Kidney Diseases: Hereditary diseases that are characterized by the progressive expansion of a large number of tightly packed CYSTS within the KIDNEYS. They include diseases with autosomal dominant and autosomal recessive inheritance.Angiotensinogen: An alpha-globulin of about 453 amino acids, depending on the species. It is produced by the liver and secreted into blood circulation. Angiotensinogen is the inactive precursor of natural angiotensins. Upon successive enzyme cleavages, angiotensinogen yields angiotensin I, II, and III with amino acids numbered at 10, 8, and 7, respectively.Captopril: A potent and specific inhibitor of PEPTIDYL-DIPEPTIDASE A. It blocks the conversion of ANGIOTENSIN I to ANGIOTENSIN II, a vasoconstrictor and important regulator of arterial blood pressure. Captopril acts to suppress the RENIN-ANGIOTENSIN SYSTEM and inhibits pressure responses to exogenous angiotensin.
The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen. (+info)
In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage. (+info)
Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps. (+info)
Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking. (+info)
Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s). (+info)
Diphtheria toxin effects on human cells in tissue culture. (6/155046)HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin. (+info)
The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites. (+info)
The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones. (+info)
TY - JOUR. T1 - Lipopolysaccharide extends the lifespan of mouse primary-cultured microglia. AU - Kaneko, Yoko S.. AU - Nakashima, Akira. AU - Mori, Keiji. AU - Nagatsu, Toshiharu. AU - Nagatsu, Ikuko. AU - Ota, Akira. PY - 2009/7/7. Y1 - 2009/7/7. N2 - Microglial activation has been implicated in the recognition and phagocytic removal of degenerating neurons; however, this process must be tightly regulated in the central nervous system, because prolonged activation could damage normal neurons. We report that mouse primary-cultured microglia, which are destined to die within a few days under ordinary culture conditions, can live for more than 1 month when kept activated by lipopolysaccharide (LPS) treatment. Primary-cultured microglia treated with sublethal doses of LPS remained viable, without any measurable increase in apoptotic or necrotic cell death. LPS-treated microglia had an arborescent shape, with enlarged somata and thickened cell bodies. Although the amount of intracellular ATP in ...
Langford test 1 (from quizlet) - infectious diseases 4180 with at muscles. Human Muscle Cell Can Perform Quizlet pleasant in order to the web site, within this moment I will explain to you with regards to Human muscle cell can perform quizlet.. And from now on, this can be the very first picture, human muscle cells can perform quizlet :. ...
The present study was undertaken to elucidate different requirements for CD2-mediated activation of naive (CD45RO-) and memory (CD45RO+) CD4+ T cells. A mitogenic combination of anti-CD2 (anti-T11(2) and anti-T11(3] mAbs could effectively induce the proliferation of memory CD4+ T cells even in the absence of monocytes. In marked contrast, naive CD4+ T cells did not disclose any proliferative responses to anti-CD2 mAbs, when monocytes were absent in culture. This differential responsiveness of naive and memory CD4+ T cells appeared to be related largely to a difference in IL-6-producing ability between both populations. IL-6 among monocyte-derived cytokines could correct unresponsiveness of naive CD4+ T cells to anti-CD2 stimulation. Unlike naive CD4+ T cells, memory CD4+ T cells produced IL-6 by themselves, with its mRNA being expressed on anti-CD2 stimulation. Anti-IL-6R mAb significantly inhibited proliferation of memory CD4+ T cells seen in the anti-CD2-stimulated cultures without monocytes, ...
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Get human primary cells derived from normal and diseased tissue. Replicate the in vivo environment as closely as possible for more relevant results.
Microbiology culture preparation. Person using a bunsen flame to sterilise the top of a jar containing a bacterial culture. - Stock Image C024/5723
We offer 4 types of normal human primary cells in the adipose cell system including endothelial cells, preadipocytes and mesenchymal stem cells.
Growth Requirements. The culture media used for cell cultures are generally quite complex, and culture condition widely varies for each cell type. However, media generally include amino acids, vitamins, salts (maintain osmotic pressure), glucose, a bicarbonate buffer system (maintains a pH between 7.2 and 7.4), growth factors, hormones, O2 and CO2. To obtain best growth, addition of a small amount of blood serum is usually necessary, and several antibiotics, like penicillin and streptomycin are added to prevent bacterial contamination.. Temperature varies on the type of host cell. Most mammalian cells are maintained at 37oC for optimal growth, while cells derived from cold- blooded animals tolerate a wider temperature range (i.e. 15oC to 26oC). Actively growing cells of log phage should be used which divide rapidly during culture.. Process to obtain primary cell culture. Primary cell cultures are prepared from fresh tissues. Pieces of tissues from the organ are removed aseptically; which are ...
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping.
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. ref Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping.
CellSeed, in Japan, is developing a series of cell therapies based on its regenerated human cell culture technology (UpCell®) for the potential treatment of
Given the fact that IL-10 is especially produced by immune cells, we investigated whether the same is true for the other members of the IL-10 family, IL-19, IL-20, IL-22, mda-7, and AK155, and, if so, to what extent. Monocytes, NK cells, B cells, and T cells from the blood of different healthy donors were investigated for their possible constitutive, as well as activation-induced, expression of these molecules. For this purpose, cells were cultured in a kinetic approach in the absence and presence of the typical cell-specific stimuli LPS (monocytes), IL-2/IL-12 (NK cells), fixed S. aureus (B cells), and anti-CD3 mAb coated on the culture vessel (T cells), respectively, for 2, 6, and 18 h. The activation state of control and stimulated cells was verified by the absence and presence of high TNF-α production in the monocyte culture and elevated expression of different activation markers, e.g., CD69, on the lymphocyte populations (Fig. 1⇑A). Gene expression of the IL-10 family members was ...
Ongoing advances in quantitative molecular- and cellular-biology highlight the need for correspondingly quantitative methods in tissue-biology, in which the presence and activity of specific cell-subpopulations can be assessed in situ. However, many experimental techniques disturb the natural tissue balance, making it difficult to draw realistic conclusions concerning in situ cell behaviour. In this study, we present a widely applicable and minimally invasive method which combines fluorescence cell labelling, retrospective image analysis and mathematical data processing to detect the presence and activity of cell subpopulations, using adhesion patterns in STRO-1 immunoselected human mesenchymal populations and the homogeneous osteoblast-like MG63 continuous cell line as an illustration.. Adhesion is considered on tissue culture plastic and fibronectin surfaces, using cell area as a readily obtainable and individual cell specific measure of spreading. The underlying statistical distributions of ...
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
Flask for cell culture are available now at Wheaton in many size and styles, also with customization options. Try the quality of our products simply signing up or calling today at (800) 2251437.
The PromoCell immuno-oncology portfolio contains characterised human primary cells, including vascular and microvascular endothelial cells, mononuclear cells, macrophages, monocytes and blood progenitor cells - plus optimized cell culture growth medium for each cell type.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
XTerra Prep columns bring the benefits of Hybrid Particle Technology to isolation and purification applications in discovery and development laboratories. XTerra Prep MS columns possess our patented Hybrid Particle Technology, with particles engineered specifically for prep. It allows you to routinely operate at pH 1-12 so you can load up to 60X more onto the column. More capacity results in fewer injections for the same amount of pure material or more material with the same number of injections. In the discovery world, this greater capacity means you can use a shorter column, producing the same amount of material in half the time.
8. This allows further bacterial multi- plication topama subsequent production of an increased amount of inflammatory mediators via the complement system and other pathways.
A self-contained mini-greenhouse useful for propagating cuttings, germinating seeds, and acclimating tissue-cultured plants intended for transfer to soil. Includes plastic tray …
MaxATP has what you need to achieve optimum physical performance during work, play, and the demands of the day. Natural performance comes from your bodys natural energy source, cellular production of Adenosine triphosphate (ATP).
Study Flashcards On PA 2008- Patho ch. 2 at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Study Flashcards On week 3 patho at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
The human system, critical infrastructure, environment, human society, human etc. are represented by system of systems, i.e. they consist...
Cell culture media and reagents for the stimulation, expansion, and differentiation of human and mouse primary cells, including immune cells, stem cells, and neural cells. - Deutschland
هدف: سلولهای بنیادی پرتوان مشتق شده از بافت بیضه، یک منبع بسیار غنی و جدیدی برای سلول درمانی در پزشکی بازساختی هستند. مطالعات اخیر نشانگر آن است که سلولهای بنیادی اسپرماتوگونی در شرایط آزمایشگاهی میتوانند به سلولهای بنیادی شبه جنینی تغییر یابند.استفاده از امواج فراصوت با شدت پایین، آثار مثبتی روی رشد و تمایز سلولها دارد. هدف از این مطالعه تأثیر امواج فراصوت روی کلونیزایی سلولهای بنیادی شبه جنینی است. مواد و روشها: ابتدا، سلولهای بنیادی اسپرماتوگونی از بیضه نوزاد موش جدا شدند. بعد از کشت دادن این سلولها در محیط DMEM/F12، حاوی 15 درصد سرم جنین
The purpose of the present investigation was to explore the effects of well-defined flow conditions on the activity of tissue factor (TF) expressed on the surface of cultured rat vascular smooth muscle cells. Cells were cultured to confluence on Permanox brand slides and stimulated to express TF by a 90 min incubation with fresh growth medium containing 10 percent calf serum. The stimulated cells were then placed in a parallel plate flow chamber and perfused with Hanks Balanced Salt Solution containing factor VIIa, factor X (FX), and calcium. The chamber effluent was collected and assayed for factor Xa (FXa) and the steady-state flux of FXa was calculated. The flux values were 68.73, 94.81, 139.75, 138.19, 316.82, and 592.92 fmole/min/cm2 at wall shear rates of 10, 20, 40, 80, 320, and 1280 s−1 respectively. The FXa flux depended on the wall shear rate to a greater degree than predicted by classical mass transport theory. The flux at each shear rate was three to five times less than that ...
TY - JOUR. T1 - Modulation of collagen synthesis by tumor necrosis factor alpha in cultured vascular smooth muscle cells. AU - Hiraga, Syouichi. AU - Kaji, Toshiyuki. AU - Ueda, Yoshimichi. AU - Zisaki, Fumiko. AU - Iwata, Kazushi. AU - Koizumi, Fumitomo. AU - Okada, Yasunori. AU - Katsuda, Shogo. AU - Nakanishi, Isao. PY - 1999/12/10. Y1 - 1999/12/10. N2 - Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. The synthesis of collagenase-digestible proteins (CDP) and noncollagenous proteins (NCP) was evaluated by the [3H]proline incorporation. It was shown that TNF-α markedly suppresses the incorporation of [3H]proline into both CDP and NCP in confluent cultures of SMCs but not in sparse cultures of the cells. Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. In confluent SMCs, the synthesis of CDP was more ...
I am planning to set up a co-culture system for human endothelial cells and human VASCULAR smooth muscle cells. I would be really interested to find out about methods of extraction and primary culture of human vascular smooth muscle cells, if anyone can help and advise, please mail me! Thanks Pippa Deex ...
Vascular smooth muscle (VSM) cell proliferation contributes to the pathogenesis of atherosclerosis, restenosis after angioplasty and vein graft disease. The regulation of genes involved in VSM cell proliferation, particularly by naturally occurring inhibitors, is therefore of some importance. We have investigated the role of the c-myc proto-oncogene in growth arrest of exponentially proliferating rat VSM cells, following mitogen withdrawal, treatment with heparin (50 micrograms/ml), interferon-gamma (IFN-gamma) (100 i.u./ml), or the cyclic nucleotide analogues, 8-bromo-adenosine-3′5′-cyclic monophosphate (8-Br-cAMP; 0.1 mM) and 8-bromoguanosine-3′5′-cyclic monophosphate (8-Br-cGMP; 0.1 mM). Growth arrest was accompanied by down-regulation of c-Myc protein and mRNA following treatment with all inhibitors. Serum withdrawal or IFN-gamma treatment suppressed c-myc expression by more than 50% within 2 h, and this occurred throughout the cell cycle. Platelet-derived growth factor, epidermal ...
The concept of arterial SMC heterogeneity has gained wide acceptance in the last years.1 2 33 The distinct phenotypes of arterial SMCs have been mainly identified in vitro,4 5 6 7 8 9 10 11 12 13 14 15 16 suggesting that specific features of SMC populations arise and are maintained in the particular environment of cell culture. Hence, it was of interest to investigate whether in vitro SMC phenotypes are preserved when SMCs are placed back in an in vivo environment. For this purpose, we have implanted 2 SMC populations exhibiting distinct levels of differentiation in vitro into the rat carotid artery submitted to endothelial injury.24 25 The implanted SMCs were marked with PKH-26, a lipophilic cell membrane linker that is halved with each cell division but is not lost from the cell membrane.34 Our results show that the 2 implanted populations essentially retain for 20 days in vivo the phenotype that they specifically exhibited in vitro.. Spindle-shaped and epithelioid rat SMC populations have ...
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Jochen Meier Ivonne Strmel Radu Iosub Sonja Schmidt and Rosemarie Grantyn Developmental Physiology Johannes Mller Institute Humboldt University Medical School (Charit) Berlin Germany Efficient delivery of DNA to primary neuronal cell cultures is of critical importance,Effectene,Transfection,Reagent,provides,efficient,gene,delivery,,,,,,,,,,,,to,primary,neuronal,cell,cultures,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Jacobs, B B., "In vivo assay of function of mouse ovaries following culture in hormone enriched medium." (1963). Subject Strain Bibliography 1963. 546 ...
Full Text - Hydrogen peroxide (H2O2) induces oxidative injury to human osteoblasts. The expression and potential function of circular RNA HIPK3 (circHIPK3) in H2O2-treated human osteoblasts were tested. We show that H2O2 significantly downregulated circHIPK3 in OB-6 cells and primary human osteoblasts. Furthermore, circHIPK3 levels were decreased in the necrotic femoral head tissues of dexamethasone-treated patients. In OB-6 osteoblastic cells and primary human osteoblasts, forced overexpression of circHIPK3 by a lentiviral construct alleviated H2O2-induced viability reduction, cell death and apoptosis. Contrarily, circHIPK3 silencing by targeted shRNA potentiated H2O2-induced cytotoxicity in OB-6 cells and primary human osteoblasts. Moreover, circHIPK3 downregulation by H2O2 induced miR-124 accumulation in OB-6 cells and primary human osteoblasts. On the contrary, miR-124
Many studies have shown that TRAIL is a potent apoptosis inducer in malignant cells, whereas its role in normal cell physiology is much less well understood.1 We have demonstrated here that both aortic endothelial cells and HUVECs exhibit a similar pattern of surface TRAIL-R expression. Moreover, in vascular endothelial cells, TRAIL stimulates the phosphorylation of the serine/threonine kinase Akt in a manner dependent on PI3K activation.14 The ability of TRAIL to activate the antiapoptotic PI3K/Akt pathway in endothelial cells is a completely new and unexpected finding, also in consideration of the large number of studies underlining the proapoptotic activity of TRAIL, at least in malignant cells.1,4,5 It should be underlined that the PI3K/Akt pathway is of central importance in endothelial cell biology, conferring survival to endothelial cells in response to angiogenic cytokine stimulation, fluid shear stress, and matrix attachment signals. This pathway is essential also for endothelial cell ...
TY - JOUR. T1 - Activation of caspase-8 and Erk-1/2 in domes regulates cell death induced by confluence in MDCK cells. AU - Chang, Yung Heng. AU - Lin, Hsi Hui. AU - Wang, Yang Kao. AU - Chiu, Wen Tai. AU - Su, Hsiao Wen. AU - Tang, Ming Jer. PY - 2007/4/1. Y1 - 2007/4/1. N2 - Under normal culture conditions, cells adhere to culture dish, spread out, proliferate, and finally cover all areas and reach confluence. During the confluent stage, cell proliferation ceases and differentiation is enhanced. Meanwhile, cell death also appears as the monolayer confluence proceeds. To delineate the mechanism of cell death induced by the confluent process, we employed Madin-Darby canine kidney (MDCK) cells. When approaching confluence, MDCK cells exhibited increase the levels of caspase-2 and enhanced the activity of caspase-8. Using various caspase inhibitors to block apoptosis, we found that only z-VAD-fmk and z-IETD-fmk can inhibit confluent cell death, indicating that confluent cell death is mediated by ...
In the present article, we compared the antiangiogenic activity of trans- and cis-resveratrol on endothelial cells of different origin activated by endogenous or exogenous stimuli. The results indicate that trans-resveratrol affects endothelial cell proliferation and various cell proliferation-independent aspects of the angiogenesis process, including endothelial cell sprout formation, collagen gel invasion, and morphogenesis. Thus, trans-resveratrol acts as an angiostatic molecule on multiple targets of the angiogenic process. In all the assays, the cis stereoisomer exerted only a limited effect, if any. In keeping with the in vitro observations, trans-resveratrol was more effective than the cis isomer in inhibiting angiogenesis and tumor growth in vivo.. The molecular bases of the antiangiogenic activity of resveratrol are poorly understood (16). trans-Resveratrol is endowed with a significant antioxidant and anti-inflammatory activity (8, 9). However, no direct relationship exists between the ...
TY - JOUR. T1 - Recombinant murine and human IL 1α bind to human endothelial cells with an equal affinity, but have an unequal ability to induce endothelial cell adherence of lymphocytes. AU - Thieme, T. R.. AU - Hefeneider, S. H.. AU - Wagner, C. R.. AU - Burger, D. R.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - Consistent with the reports of others, we have demonstrated that human peripheral blood lymphocytes adhere to cultured human umbilical vein-derived endothelial cells (EC) in vitro. In our studies adherence was increased twofold to threefold by a 6-hr preincubation of the EC with IL 1. Recombinant human IL 1α induced a maximal adherence response at less than 1 U per 2 x 104 EC. In contrast, recombinant murine IL 1α was found to be 250- to 1250-fold less active in the adherence assay, based on units of IL 1 activity defined by the murine thymocyte proliferation assay. Moreover, when EC were preincubated with excess murine IL 1, no inhibition of the adherence-inducing effect of human IL 1 was ...
The effects of interferons (IFNs) on lymphocyte adhesion to cultured human vascular endothelial cells (EC) were investigated using an in vitro assay. Endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (CM) from 12-O-tetra decanoylphorbol 13-acetate-concanavalin A (TPA-Con A) stimulated human peripheral blood lymphocytes (PBL), or with recombinant (r) gamma interferon (IFN-gamma) or r alpha interferon (IFN-alpha), and then were incubated with freshly isolated PBL. Natural IFN-gamma in the TPA-Con A CM and rIFN-gamma (12.5-500 U/ml) induced major histocompatibility complex-class II antigens (HLA-DR, HLA-DP, and HLA-DQ) and significant lymphocyte adhesion to the EC, whereas rIFN-alpha did not. The lymphocyte adhesion to the EC and the expression of DR antigens on the EC were well correlated in terms of both kinetics and the dose-response pattern of rIFN-gamma. When EC expressing I region associated (Ia) antigen were preincubated with ...
The fully sequenced pCERI is transformed into E.coli BL21 and E.coli Nissle 1917 that are used for the characterization of our regulatory system. The expression efficiency of the genes of interest is characterized by a fluorescence-based assay (Results) Overnight cultures of both recombinant strains are inoculated with single colonies. The overnight cultures are diluted with LB-Amp to an OD of 0.02. 200 μ of the dilutions are pipetted in microtiter plates and incubated at 37 °C in the plate reader. Every 15 minutes mRFP fluorescence (Ex. 584 nm, Em. 607 nm), CFP fluorescence (Ex. 439 nm, Em. 476) and OD600 is measured over a period of 13 h. The measured RFU (relative fluorescence unit) is either blotted against time or against OD600 to evaluate the expression behavior of pCERI. Additionally several samples are either induced with 0.1 -50 nM of 3OC6-HSL and 0.1-100 nM of C8-HSL to explore putative advantages of higher HSL-synthesis, that might be possible by positioning each HSL-synthase, ...
This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary mesenchymal cells. Each of eleven mesenchymal cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. Tissue types covered include bone, cartilage, tendons and ligaments, muscle (smooth, skeletal, and cardiac), adipose tissue, and skin (dermis), as well as multi-potent mesenchymal stem cells and fibrocytes. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for ...
Choice of AMP 100 versus AMP 50 - posted in Molecular Cloning: Ok, i know the main difference is the amp concentration. But i wonder if it matters that i use amp 50 for overnight culture versus amp 100 for overnight culture.
I am studying the differentiation of leukemic cells along the monocytic lineage using calcitriol as differentiative agent and others vitamins as possible stimulating compounds on differentiation. In my work I have used HL-60 cells as in vitro culture cells but now I have some problems because these cells have changed their response and develop expontaneous differentiation in normal culture conditions. Now, I am thinking on NB4 cells as a better cell line to study differentiation. I have ask some information to cell banks as ATCC and ECA but they dont have this cell line. Could anybody tell me if anyone can send me this cell line?, I have very gratefull if anyone could send me HL-60 cells too. Waiting positive responses and acknowledging in advance. Guillermo Lopez-Lluch Departamento de Biología Celular Facultad de Ciencias Universidad de Córdoba Avda. S. Alberto Magno S/N E-14007 Córdoba, Spain. TFNO: Int + 34-57-218634 FAX: Int + 34-57-218634 e-mail: bc2biced at lucano.uco.es ...
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TY - JOUR. T1 - Studies on the mechanism of hrmonal stimulation of zinc uptake in human cell cultures. T2 - hormone-cell interactions and characteristics of zinc accumulation.. AU - Cox, R. P.. AU - Ruckenstein, A.. PY - 1971/2. Y1 - 1971/2. UR - http://www.scopus.com/inward/record.url?scp=0015012917&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015012917&partnerID=8YFLogxK. M3 - Article. C2 - 4322943. AN - SCOPUS:0015012917. VL - 77. SP - 71. EP - 81. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 1. ER - ...
One group of projects in the lab concerns "co-receptors", cell-surface molecules that regulate the interaction between soluble growth factors and their cell-surface receptors. We have identified a novel co-receptor for BMPs (bone morphogenetic proteins) and are studying the roles of Heparan Sulfate Proteoglycans (HSPGs) as co-receptors for a variety of growth factors, including those that drive tumor development and pattern the developing nervous system. We have used "knockout" technology to generate mice that are mutant for particular HSPGs, and are also working with mice that are mutant for some of the biosynthetic enzymes required to make HSPGs. We are also using modeling approaches to understand how HSPGs influences the kinetic parameters of growth factor-receptor interaction ...
How would the cells respond to Aβ? Mancuso injected 5 μL of 10 μM synthetic oligomers into the mouse brain ventricles eight to 10 weeks after transplanting the human microglia. In reaction to this insult, endogenous mouse microglia shifted their transcriptomes sequentially from homeostatic to cytokine responsive, to activated, with the latter partially overlapping with disease-associated microglial (DAM) signatures described previously (Jun 2017 news). Human microglia in the mice underwent a transcriptional transformation as well, but analysis of more than 10,000 orthologous genes indicated poor correlation between human and mouse microglial responses. Of 207 differentially activated genes, 112 were up in human but not mouse microglia; they included GWAS hits BIN1 and PICALM. "We saw that human microglia responded very differently to Aβ oligomers. This emphasizes the need to look specifically at human cells in the context of AD," said De Strooper.. Blurton-Jones and colleagues took a ...
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Recent evidence supports a strong role for immune suppression in facilitating cancer progression. Transforming growth factor beta (TGFß) regulates a diverse array of biological events, including neoangiogenesis, stromal formation, and immune responses. Both TGFß and a small molecule inhibitor of TGFßR-1, LY364947, were effective regulators of these processes in vitro. Using quantitative RT-PCR and fluorescence imaging, TGFß increased collagen production by pancreatic stellate cells while addition of LY364947 suppressed production below basal levels. Endothelial cell proliferation, measured as a function of endothelial tube formation in Geltrex® matrix, was similarly increased by TGFß and reduced to basal levels by LY364947. To explore the in vivo effects of these molecules, LY364947 was encapsulated in liposomes to encourage tumor accumulation and to avoid administration of DMSO vehicle. Liposomes were prepared with a molar ratio of 6:3:1 1,2-dipalmitoyl-sn-glycero-3-phosphocholine ...
Cell culture. Puromycin is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic ... Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures ... "Mol Biol Cell. 8 (8): 1559-1573. doi:10.1091/mbc.8.8.1559. PMC 276176. PMID 9285825.. ... "In Hopsu-Havu, Väinö K.; Järvinen, Mikko; Kirschke, Heidrun (eds.). Proteolysis in Cell Functions. IOS Press. pp. 88-95. ISBN ...
... sometimes before other cultures (e.g. blood cultures and sputum cultures) become positive. A Gram stain may give a rough ... Cell count and differential. The number of white blood cells can give an indication of infection. The specific subtypes ... Cultures and stains. If the effusion is caused by infection, microbiological culture may yield the infectious organism ... In the case of blood: it will contain some or all plasma proteins, white blood cells, platelets and (in the case of local ...
Cell culture. Mineral oil of special purity is often used as an overlay covering microdrops of culture medium in petri ... The use of oil presents several advantages over the open culture system: it allows for several oocytes and embryos to be ... as an adjuvant to stimulate a cell-mediated immune response to the vaccinating agent. In the poultry industry ... as few as 20 microlitres per oocyte instead of several millilitres for the batch culture); and it serves as a temperature ...
Cell culture. Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering ... Cell culture (cell-based) manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines ... In contrast, cell culture manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines (e ... AVI Bio Pharma Inc. has evidence of inhibition of multiple subtypes of influenza A virus in cell culture with Morpholino ...
Cell culture. *Model organisms (such as C57BL/6 mice). *Methods *Nucleic acid ... Up-regulation is a process that occurs within a cell triggered by a signal (originating internal or external to the cell), ... Cell. 62 (5): 712-27. doi:10.1016/j.molcel.2016.04.006. PMC 5476208 . PMID 27259203.. ... Cells do this by modulating the capping, splicing, addition of a Poly(A) Tail, the sequence-specific nuclear export rates, and ...
"Labeling Cultured Cells with32Piand Preparing Cell Lysates for Immunoprecipitation". Labeling Cultured Cells with 32Pi and ... ACK is used for lysis of red blood cells in biological samples where other cells such as white blood cells are of greater ... "Lysis of Cultured Cells for Immunoprecipitation". Cold Spring Harbor Protocols. 2010 (8): pdb.prot5466. doi:10.1101/pdb. ... "Detergents for Cell Lysis and Protein Extraction". www.thermofisher.com. Retrieved 2016-03-16.. ...
... mammalian cell cultures; vegetables; human nostrils and throats; and human and animal brain, skin, and lung tissues. B. ...
Cell behavior. Main article: Microfluidic cell culture. The ability to create precise and carefully controlled ... cell separation, in particular, blood cell separation, protein analysis, cell manipulation and analysis including cell ... Microfluidic cell culture. References. *^ S.C.Terry, J.H.Jerman and J.B.Angell:A Gas Chromatographic Air Analyzer ... Fuel cells. Further information: Electroosmotic pump. Microfluidic fuel cells can use laminar flow to separate the fuel ...
10.1 Cell-of-origin. *11 Society and culture. *12 Research *12.1 CRPC ... lo Prostate Cancer Cell Population Harbors Self-Renewing Long-Term Tumor-Propagating Cells that Resist Castration". Cell Stem ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
Kampf C; Roomans GM (May 2001). "Effects of hypochlorite on cultured respiratory epithelial cells". Free Radic. Res. 34 (5): ... Nitric oxide can diffuse through the plasma membrane into neighbouring cells, allowing cell signalling, so nitroindazole ...
While the long-term potentiation of synapses in cell culture seems to provide an elegant substrate for learning and memory, the ... The synaptic tagging hypothesis attempts to solve the cell's difficult problem of synthesizing proteins in the cell body but ... When an axon of cell A is near enough to excite a cell B and repeatedly or persistently takes part in firing it, some growth ... in cells of the dentate gyrus. What Lømo unexpectedly observed was that the postsynaptic cells' response to these single-pulse ...
"BRCA1 inhibition of telomerase activity in cultured cells". Molecular and Cellular Biology. 23 (23): 8668-90. PMC 262673 . PMID ... cell cycle arrest. • positive regulation of response to DNA damage stimulus. • positive regulation of cell proliferation. • ... "Cell. 142 (3): 480-93. PMC 2923036 . PMID 20691906. doi:10.1016/j.cell.2010.06.037.. ... "Cell. 151 (1): 68-79. PMC 3471363 . PMID 23021216. doi:10.1016/j.cell.2012.08.033.. ...
Phosphate buffered saline (cell culture). *Tyrode's solution. References. *^ a b c British national formulary: BNF 69 (69 ...
For euglenids to reproduce, asexual reproduction takes place in the form of binary fission, and the cells replicate and divide ... This varies from rigid to flexible, and gives the cell its shape, often giving it distinctive striations. In many euglenids the ... which are excavate eukaryotes of the phylum Euglenophyta and their cell structure is typical of that group. They are commonly ... The pellicle is composed of proteinaceous strips underneath the cell membrane, supported by dorsal and ventral microtubules. ...
Cell culture: Human or animal cell cultures are infected with the microbe of interest. These cultures are then observed to ... These are primarily used to culture bacteria and fungi.. *Liquid culture: Cells are grown inside a liquid media. Microbial ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ... Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ...
... which has been found in one study to be mutagenic and genotoxic in cell cultures which do not respond to β-carotene itself. ... "Toxicity of oxidized β-carotene to cultured human cells". Experimental Eye Research. 81 (2): 239-243. doi:10.1016/j.exer. ...
G. Diets, Culture Media, Food Supplements. Vol. 4. Culture Media for Cells, Organs and Embryos. Cleveland, OH: CRC Press, 1977 ... G. Diets, Culture Media, Food Supplements. Vol. 3. Culture Media for Microorganisms and Plants. Cleveland, OH: CRC Press, 1978 ... G. Diets, Culture Media, Food Supplements. Vol. 1. Dits for Mammals. Cleveland, OH: CRC Press, 1977. 645 pp. CRC Handbook ... G. Diets, Culture Media, Food Supplements. Vol. 2. Food Habits of and Diets for Invertebrates and Vertebrates. Zoo diets. ...
Predieri, S. (2001). "Mutation induction and tissue culture in improving fruits". Plant Cell, Tissue and Organ Culture. 64 (2/3 ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... Koornneef, M.; Meinke, D. (2010). "The development of Arabidopsis as a model plant". The Plant journal : for cell and molecular ... DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under ...
Ader M., Tanaka E. M. (2014). "Modeling human development in 3D culture". Current Opinion in Cell Biology. 31: 23-28. doi: ... This occurs when individual cells or groups of cells grow longer. Not all plant cells will grow to the same length. When cells ... These cell divisions are usually rapid with no growth so the daughter cells are half the size of the mother cell and the whole ... A single tissue, formed from a single type of progenitor cell or stem cell, often consists of several differentiated cell types ...
Landecker, Hannah (2007). Culturing life: how cells became technologies. Cambridge, MA: Harvard University Press. p. 49. link. ... contrary to the claim that the avian egg cell (in strict sense) and its yolk are a single giant cell. ... The yolk mass, together with the egg cell or ovum properly (after fertilization, the embryo) are enclosed by the vitelline ... The yolk is not living cell material like protoplasm, but largely passive material, that is to say deutoplasm. The food ...
Predieri, S. (2001). "Mutation induction and tissue culture in improving fruits". Plant Cell, Tissue and Organ Culture. 64 (2/3 ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... DNA is bound to tiny particles of gold or tungsten which are subsequently shot into plant tissue or single plant cells under ... The accelerated particles penetrate both the cell wall and membranes. The DNA separates from the metal and is integrated into ...
"Plant Cell, Tissue and Organ Culture. 72 (1): 95-98. doi:10.1023/A:1021203811457. Retrieved 19 May 2016.. ... "The Plant Cell. 14 (suppl 1): S153-S164. doi:10.1105/tpc.000679. ISSN 1532-298X. PMC 151253 . PMID 12045275. Retrieved 26 May ... Induces Apoptosis and Cell Cycle Arrest in A549 Cells through p53 Accumulation via c-Jun NH2-Terminal Kinase-Mediated ... Venus flytraps can also be propagated in vitro using plant tissue culture. Most Venus flytraps found for sale in nurseries ...
Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... culture worldwide via inhalation or skin lesion Babesiosis Babesia B. divergens, B. bigemina, B. equi, B. microfti, B. duncani ... culture worldwide contact lenses cleaned with contaminated tap water Granulomatous amoebic encephalitis Balamuthia mandrillaris ... red blood cells Giemsa-stained thin blood smear New England (different species have worldwide distribution) tick bites, e.g. ...
Cell cultureEdit. Petri dishes are also used for cell cultivation of isolated cells from eukaryotic organisms, such as in ... alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to culture ... Axenic Cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish ... In the latter, the cells often grow as a layer attached to the bottom surface of the dish, below the culture medium. ...
"Cell Stem Cell. 10 (5): 556-69. doi:10.1016/j.stem.2012.03.009. PMC 3348510 . PMID 22560078.. ... Society and cultureEdit. People with prostate cancer generally encounter significant disparities in awareness, funding, media ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
If the stem cells and sebaceous gland are destroyed, there is then no possibility for regeneration of the hair follicle, and ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ... The goal of treatment is to decrease or eliminate the lymphocytic inflammatory cells that are attacking and destroying the hair ... However, all cicatricial alopecias involve inflammation directed at the upper part of the hair follicle where the stem cells ...
Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... Cell culture technologies at that time didnt allow for good data reproducibility, because stem cells were cultured in 2D. ... 14:10 PANEL DISCUSSION: Will 3D Cell Culture replace animal models?. * Advantages and disadvantages of 3D Cell Culture vs ... He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell ...
A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon.. Merolli A1, Mao Y1, Voronin ... Schwann cell wrapping.. Top left: a schematic drawing of a Schwann cell wrapping around an axon (black) in transverse section ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ... A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon ...
Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly by macrophages. Our cell ... Cell culture study * 1. Cell Culture studiesCell Culture studies -We have previously shown SPIO particles are taken up avidly ... 2. Cell Culture StudiesCell Culture Studies We have Comparison of the 3 different SPIOs in the cell culture, and their effect ... Our cell culture studies showed a transient elevation in free radical of macrophages with a rapid return to the baseline. -Next ...
3D Cell Culture. 3D Cell Culture. Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ... Cell Culture. Cell Culture. Create a more in vivo-like cell culture to advance your discoveries right from the start. ... 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many ... Sophisticated in vitro 3D cell culture models have a major impact on advancing cell culture. ...
In Epithelial Cell Culture Protocols, a team of well-versed experimenters and cli ... Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. ... Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium ... and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use ...
... a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques f ... New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. ... Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach ... Well-Differentiated Human Airway Epithelial Cell Cultures M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. ...
Cell Culture Flow Systems. Lonza For info: 800-638-8174 www.lonza.com ... The system consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over ... Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve ... including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. Not only is the system ...
... Stem Cell Training course: Human Pluripotent Stem Cells in Culture. 2017-2018 - Course ... basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). The ... Assessment of PSC culture. • Differentiation of human ES cells: Formation of spontanteous embryoid boides (EB) and defined EBs ... Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells.. Practical ...
Tissue culture *Two separate TC labs for ML-I and ML-II tissue culture ...
... buffer Cancer cell culture cell density cell growth cell lines cell numbers cell population cell suspension cell type ... culture.html?id=g5xqAAAAMAAJ&utm_source=gb-gplus-shareAnimal cell culture. ... Cell culture. Language Arts & Disciplines / Linguistics / General. Medical / Genetics. Science / Life Sciences / Cytology. ... Animal Cell Culture: A Manual of Basic Techniques. Tajamul Islam Shah. Limited preview - 2018. ...
I am interested in staining the cells alive by adding Xgal to the culture ,, media without fixing the cells. Is it possible? ... beta galactosidase in cultured cells. Martin Kennedy mkennedy at chmeds.ac.nz Tue Oct 5 21:23:18 EST 1993 *Previous message: ... Martin Leach Hi, Ive done X-gal staining on fixed mamalian cells expressing lacZ, but can you really do it on live cells ... Does anybody know of a reference for staining mammalian cells transfected ,, with betagalactosidase constructs? ,, ...
The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor ... www.concordia.ca/content/concordia/en/research/genomics/facilities-instruments/cell-culture.html ... Dedicated culture rooms. GE S110.01, SP 550.04. SP 430.01 (Whiteway).. Incubation Shakers. GE S110.05, GE S110.09, GE 230.00, ... Each culture room is equipped with two biological safety cabinets, a stack of three large incubation shakers, a refrigerated ...
... ELIZ at CCIT.ARIZONA.EDU ELIZ at CCIT.ARIZONA.EDU Thu Nov 12 11:08:14 EST 1992 *Previous message: ct protein ... Networkers, I am interested in knowing how easy it would be to set up cell cultures from any transformed line of Arabidopsis. ...
Cells, cultured synonyms, Cells, cultured pronunciation, Cells, cultured translation, English dictionary definition of Cells, ... cultured. n. 1. The technique or process of growing bacterial or fungal cells or cells derived from tissues of living organisms ... cell culture. (redirected from Cells, cultured). Also found in: Medical, Encyclopedia. cell culture. n.. 1. The technique or ... Cells, cultured - definition of Cells, cultured by The Free Dictionary https://www.thefreedictionary.com/Cells%2c+cultured ...
... in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability ... We describe a method of growing an animal cell in a culture medium, ... Specific examples of cells include CHO cells, HeLa cells, HEK cells, HEK 293 cells, COS1 cells, embryonic stem cells, African ... Animal Cell The improved cell culture medium is suitable for the culture of any cell or colony or cell line preferably of ...
KudoZ) English to Spanish translation of early-passage primary human cells cultured [cell culturing - Biology (-tech,-chem, ... Medical - Biology (-tech,-chem,micro-) / cell culturing. English term or phrase: early-passage primary human cells cultured. ... Containing early-passage primary human cells cultured +/- stimulus combinations. -Concentrations covered reported clinical ... BIOMAP systems: Model complex signaling networks in primary human cell types to validate compounds and targets, identify ...
The Maxwell RSC Cultured Cells DNA Kit is used with the Maxwell RSC Instrument to provide a simple method for efficient, ... automated extraction of DNA from up to 5 × 10^6 mammalian tissue culture cells and 2 × 10^9 bacterial cells. ... Automated Benchtop DNA Purification from Mammalian and Bacterial Cultured Cells The Maxwell® RSC Cultured Cells DNA Kit is used ... Purification of DNA from 3D cell cultures using the Maxwell® RSC Cultured Cells DNA Kit ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Features a superior cell culture surface with demonstrated performance that elevates stem cell and cell culture research. ... Thermo Scientific™ BioLite Cell Culture Treated Dishes Perform general cell culture procedures more economically with Thermo ... Corning™ TC-Treated Culture Dishes Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. ...
... , Date: 3 Sep 93 05:03:13 GMT , I know that plant cell and tissue culture has been around for long time. , , ... How is it that high value products such as vanilla, taxol, quinine etc , are not widely produced by plant cell culture ? ... Plant cell culture. SPLUHAR at CROP.UOGUELPH.CA SPLUHAR at CROP.UOGUELPH.CA Sun Sep 5 23:19:15 EST 1993 *Previous message: ... An example of such unstable types of cells is Alfalfa suspension cells, which frequently loose chromosomes and double others, ...
... Steven McClue Genetics sjm at mole.bio.cam.ac.uk Wed Jun 1 04:10:46 EST 1994 *Next message: P-element ... al type cells. Any info on where these can be obtained, along with any special conditions for culturing, would be gratefully ... Can anyone tell me where I can find a source for Drosophila (stable) cell lines ? Im interested in getting hold of some ...
Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and ... Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used ... Used for culture of variety of cell types under low serum conditions. Corning™ DMEM/Ham's F-12 50/50 Mix, Powder ensures ...
Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). ... This webinar will introduce basic concepts of cell cultur... ... Cell culture is a fundamental technique for a variety of ... Cell Culture Fundamentals. Cell culture is a fundamental technique for a variety of applications. Cells are cultured in ... Introduction to cell culture. •Critical factors for successful cell culture. •Cell culture tips and techniques. Presenter Bio: ...
... including how the heart functions and how cells that create heart tissue behave. Scientists studying these changes on the ...
It cannot regenerate itself, because it is formed by a layer of cells that is lost by the time the tooth appears in the mouth. ... the feeder-layer cells are known as the 3T3-J2 cell line). This crucial step boosts the number of dental epithelial cells ... the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of ... H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 ...
Cell Culture Media and Reagents. Cells from the adult RPE cell line ARPE-19 were obtained from the American Type Culture ... Cell Culture. Adult human retinal pigment epithelial (ARPE-19) cells were routinely cultured in 95% air and 5% CO2 at 37°C in ... Viability of Cultured ARPE-19 Cells following Storage. To study the impact of different temperatures on RPE cell survival, cell ... In the current study, ARPE-19 cells were cultured on glass or plastic culture dishes, reducing the culture variables to a ...
DifferentiationTest includes cryopreservation of culturedAssaysBiologyEmbryonicCryopreservationMethodsProliferationVivoTissuesMonolayerViabilityIsolationEndothelialProliferateProteinFeeder2019GrownLaboratoryTransfectionRegenerative medicineApplicationsUseful hints for culturing anScaffolds2018Skeletal muscleBiochemicalContaminationInducesNovel 3D cell cultureDifferentiateViableTransplantationResearchersProtocolsMolecularTissue and organ cultureGlialWebinarFinitePluripotent cell linesMediaGeneticPlant cell culturesSuitableBiolReagentsAirway epithelial cellsSerumStructuralMulticellularAnimalBehaviorSecretion
- Yet with the rise of promising new opportunities within such fields of study as cancer research and stem cell differentiation, using 2D models to obtain useful information about in vivo biological processes has created challenges. (corning.com)
- But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. (nih.gov)
- In basic research, 3D cell culture is being used to establish models of disease and unravel the mysteries of differentiation. (genengnews.com)
- With the proper combinations of growth and differentiation factors, mouse ES ( 22 , 23 ) and EG (S.W., unpublished results) cultures can generate cells of the hematopoietic lineage and cardiomyocytes ( 24 , 25 ). (pnas.org)
- Oxygen-sensing transcription systems, such as hypoxia-inducible factors, play an important role in regulating the differentiation of stem cells ( 3 ), as does the distribution of diffusing growth factors. (sciencemag.org)
- However, the complexity, variability, and ill-defined nature of these interactions make it difficult to understand the proliferation, differentiation, and migration of cells embedded within these natural gels. (sciencemag.org)
- As SCs can only accommodate the differentiation of a finite number of germ cells [ 7 ], a deregulation in SCs apoptosis could affect spermatogenesis, resulting in a lower fertility or even in infertility. (hindawi.com)
- and growth, differentiation and organogenesis in plant tissue and organ cultures. (elsevier.com)
- We are interested in understanding and elucidating transcriptional and/or translational events involved in gene regulation as well as unraveling the genetic, molecular and physiological determinism of growth and differentiation of plant cells, tissues and organs cultured in vitro. (springer.com)
- A couple of weeks ago, I was giving a plate of undifferentiated stem cells to practice passaging and removing unwanted differentiation. (bio-link.org)
- Under terms of the formal agreement, the agencies agreed upon a joint regulatory framework wherein FDA oversees cell collection, cell banks, and cell growth and differentiation. (foodengineeringmag.com)
- She has worked with xenograft models and many cancer cell lines, and she has expertise in in vitro cell based functional assays and in vivo experiments. (brighttalk.com)
- In addition to spheroids, collagen-based scaffolds that encourage cell aggregation into tumoroids have been used for immunotherapy applications such as natural killer cell cytotoxicity assays. (genengnews.com)
- Physiological relevance is a key parameter to improve predictivity of cell-based assays in drug discovery and toxicity testing for compound. (dechema.de)
- In culture assays it was possible to test human and sheep agent strain combinations that could not be discriminated in animals. (medicalnewstoday.com)
- The µ-Dish 35-mm Quad from Ibidi GmbH is a four-compartment cell culture dish that can be used for simultaneous assays, guaranteeing high optical quality. (photonics.com)
- Mutation assays in cultured cells of transgenic rodents constitute, in many ways, viable alternatives to in vivo mutagenicity experiments in the corresponding animals. (mdpi.com)
- Nuclear runoff assays documented an increased transcriptional rate for the HB-EGF gene in lyso-PC-treated cells. (jci.org)
- This week (and a half) in Portland Community College's Bioscience Technology Program we continued work with assays, and had guest speakers in Current Topics and Cell Culture. (bio-link.org)
- He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell research. (smi-online.co.uk)
- Scientific advances in cancer cell biology are changing the paradigm in oncology research. (corning.com)
- Current preclinical GBM models are limited by the lack of a "normal" human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. (brighttalk.com)
- This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians. (wiley-vch.de)
- Membrane and organelle assembly has emerged as a dominant theme in cell biology of the twenty-first century. (nature.com)
- Classical cell biology teaches that exocytosis causes the membrane of exocytic vesicles to disperse into the cell surface and that a cell must later retrieve by molecular sorting whatever membrane components it wishes to keep inside. (pnas.org)
- The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (encyclopedia.com)
- Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (elsevier.com)
- The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (strath.ac.uk)
- We propose to apply a systems biology approach to achieve a computational representation of tissue-level physiological responses by integrating empirical data derived from organotypic culture systems with computational models of intracellular pathways to better predict human responses. (osti.gov)
- This is a hands-on, basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). (ucl.ac.uk)
- Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (fishersci.com)
- To address these limitations, our guest presenter and her team have established a unique model system whereby they can retro-engineer patient-specific GBMs using patient-derived glioma stem cells (GSCs) and human embryonic stem cell (hESC)-derived cerebral organoids. (brighttalk.com)
- A pioneering new study led by Florida State University biologists could fundamentally change our understanding of how embryonic stem cells differentiate into specific cell types. (news-medical.net)
- Science Daily reports that for the first time, human embryonic stem cells have been cultured under chemically controlled conditions without the use of animal substances, which is essential for future clinical uses. (slashdot.org)
- Now, for the first time, we can produce large quantities of human embryonic stem cells in an environment that is completely chemically defined," says professor Karl Tryggvason, who led the study at Sweden's Karolinska Institutet. (slashdot.org)
- IPsC are when they take cells from your own body and make them revert back to a similar state to embryonic stem cells, to where they can then be turned into any cell type you want (the advantage there being they're your cells so you wouldn't get tissue rejection like you would with embryonic stem cells. (slashdot.org)
- Over a period of 7-21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. (pnas.org)
- Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81) that have been used routinely to characterize embryonic stem and EG cells. (pnas.org)
- Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. (pnas.org)
- Pluripotent stem cells have been derived from two embryonic sources. (pnas.org)
- Embryonic stem (ES) cells are derived from the inner cell mass of preimplantation embryos ( 1 , 2 ), and embryonic germ (EG) cells are derived from primordial germ cells (PGCs) ( 3 , 4 ). (pnas.org)
- Other important characteristics include growth as multicellular colonies, normal and stable karyotypes, the ability to be continuously passaged, and the capability to differentiate into cells derived from all three embryonic germ layers. (pnas.org)
- This property alone may not be a definitive test of stem cell pluripotency, as it has been demonstrated that rat and mouse visceral (yolk sac) endoderm are capable of forming highly differentiated teratomas containing cells of all three embryonic germ layers ( 29 , 30 ). (pnas.org)
- While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. (springer.com)
- Via homologous recombination in embryonic stem cells (ES cells), it is possible to generate a specific genetic modification at a specific location in the genome. (dkfz.de)
- 2. The method of claim 1 , wherein the human pluripotent stem cells are embryonic stem cells. (google.es)
- The invention relates to stem cells (SC) in particularly to methods and systems for handling human embryonic stem cells (hESC). (google.es)
- Embryonic stem cell lines derived from human blastocysts. (google.es)
- Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. (google.es)
- Feeder-free growth of undifferentiated human embryonic stem cells. (google.es)
- Embryonic stem cells differentiate more efficiently to blood-forming stem cells when cultured in three-dimensional scaffolds compared to cells cultured in two dimensions ( 2 ). (sciencemag.org)
- At the same time, he was propagating und publicizing a new image of life that could be understood as a literal embodiment of durée in the sense of philosopher Henri Bergson: in 1912, Carrel placed tissue from an embryonic chicken heart in a specifically designed flask and maintained this living culture for over 20 years. (jhu.edu)
- In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (wikipedia.org)
- Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. (springer.com)
- This guide contains general technical information for working with animal cells in culture, including media, subculturing, cryopreservation and contamination. (atcc.org)
- Research activities are focused on the development of methods for cryopreservation and characterisation of cell cultures for the monitoring of genetic and epigenetic stability. (gbif.org)
- Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers over conventional cell culture methods. (smi-online.co.uk)
- In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. (springer.com)
- In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
- The establishment of future retinal pigment epithelium (RPE) replacement therapy is partly dependent on the availability of tissue-engineered RPE cells, which may be enhanced by the development of suitable storage methods for RPE. (hindawi.com)
- With the advancement of RPE cell replacement therapy, and with 20-25 million known sufferers from AMD worldwide [ 17 ], a great need for improved storage methods for cultured RPE is likely to emerge. (hindawi.com)
- Provided are systems and methods for providing human cell cultures. (google.es)
- Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development. (google.es)
- If you are adopting the next-generation technologies to improve your daily lives such as smart phones, then why stick to the old cell culture methods and compromise results in the lab? (lonza.com)
- Primary cell cultures come from the outgrowth of migrating cells from a piece of tissue or from tissue that is disaggregated by enzymatic, chemical, or mechanical methods. (qiagen.com)
- After the Second World War, methods of culturing tissue were further improved and provided the basis for a genuine mass (re)production of cells. (jhu.edu)
- Accordingly, most culture methods require sterile conditions. (encyclopedia.com)
- Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. (elsevier.com)
- GBI Research, the leading business intelligence provider, has released its latest research report, Manufacturing of Vaccines - Cell Culture Technology Gradually Replacing Egg-Based Manufacturing, which provides insights into the production methods of vaccines and into vaccine production capacity. (mynewsdesk.com)
- Previous experience in either microengineering systems or cell culture methods would be advantageous. (strath.ac.uk)
- Protocols in this series are examples of methods for general cell culture and have not been rigorously validated and optimized by QIAGEN. (bio-medicine.org)
- The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
- Other topic areas which will be featured at Cell Culture 2012 are the challenges involved with preventing cell line cross contamination and the methods used to mitigate these risks, as well as novel methods in the use of 3D cell cultures. (selectbiosciences.com)
- This enhances the ability for scientists to study basic biological mechanisms such as cell number monitoring, cell viability, proliferation and morphology. (smi-online.co.uk)
- Oxidative stress resulted in a profound influence on advancing the senescence (functional deterioration) of these cells and inhibiting their proliferation. (eurekalert.org)
- This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. (wiley-vch.de)
- This characteristic can be used for the indirect quantification of cell death and to determine differences in proliferation upon stimulation with death-inducing agents. (chemie.de)
- To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. (atcc.org)
- To support the proliferation and plating efficiency of renal epithelial cells (e.g. (atcc.org)
- This attachment is essential for proliferation - many adherent cell cultures will cease proliferating once they become confluent (i.e., when they completely cover the surface of cell culture vessel), and some will die if they are left in this confluent state for too long. (qiagen.com)
- Interestingly, cells engineered to express more telomerase retained telomeres and the ability for extended proliferation. (encyclopedia.com)
- 3 4 In cultured mesangial cells, ET-1 binds its specific receptors, enhances phosphoinositide turnover, and induces contraction and proliferation of these cells. (ahajournals.org)
- 3D cell culture enables cells to grow and interact with their surroundings in all three dimensions, better mimicking the in-vivo conditions in which the cells are naturally present. (smi-online.co.uk)
- Create a more in vivo-like cell culture to advance your discoveries right from the start. (corning.com)
- As research demands have become increasingly complex, we have been there every step of the way, creating 3D cell culture solutions that allow you to produce optimal environments for growing cells that exhibit in-vivo like behaviors and functionality. (corning.com)
- Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve cellular phenotypes and human in vivo relevance of in vitro cell culture models. (sciencemag.org)
- Sartorius Stedim Biotech, a leading international supplier for the biopharmaceutical industry today announced the launch of the BIOSTAT RM TX single-use bioreactor, a new wave mixed system developed specifically for closed, automated expansion of consistent quality cell products such as ex vivo cellular immunotherapies. (news-medical.net)
- It is becoming increasingly clear that these models mimic cells in vivo at a greater capacity than traditional cell culture. (genengnews.com)
- Until now it has not been possible to establish progressively growing cultures of these cells in vitro , and cell lines have only been obtained after teratocarcinoma formation in vivo . (nature.com)
- These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo . (nature.com)
- The pluripotency of ES and EG cells can be demonstrated in vitro and in vivo . (pnas.org)
- Perhaps the most definitive in vivo test of developmental potential would be a demonstrated contribution to all cell lineages in a chimeric animal, but this test is not practical or possible for all species and cannot be done with human cells. (pnas.org)
- It uses a novel method to create high-density collagen scaffolds with cells embedded, enabling improved cellular interactions that more closely resemble an in vivo environment. (lonza.com)
- Primary cells are considered by many researchers to be more physiologically similar to in vivo cells. (qiagen.com)
- Cells can be isolated from tissues for ex vivo culture in several ways. (wikipedia.org)
- 1. The technique or process of growing bacterial or fungal cells or cells derived from tissues of living organisms in a culture medium. (thefreedictionary.com)
- The cell-scaffold approach is based on tissue-engineering technology developed at the Forsyth Institute (MA) and was applied by one of the Tokyo researchers to produce enamel-like tissues in 2002. (eurekalert.org)
- Although native tissues possess subtleties that still elude 3D culture systems, scientists are making progress. (genengnews.com)
- Most cells derived from tissues are anchorage-dependent. (qiagen.com)
- The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. (elsevier.com)
- The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. (elsevier.com)
- The selection is a vital source of data for readers interested in the culture of cells and tissues. (elsevier.com)
- Rapidly isolates primary cells to consistently obtain a uniform single-cell suspension from tissues. (fishersci.ca)
- Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (wikipedia.org)
- What kinds of structural materials might be used to culture tissues (e.g., scaffolding), and are there any that could not be addressed by ordinary food ingredient safety assessment? (foodengineeringmag.com)
- For example, human breast epithelial cells proliferate abnormally (like tumor cells) when cultured as a two-dimensional monolayer, but display normal cell growth behavior and form structures typical of breast tissue when cultured in three-dimensional membranes that resemble their native environment ( 1 ). (sciencemag.org)
- Depending on their origin, animal cells grow either as an adherent monolayer or in suspension. (qiagen.com)
- Adherent cells are anchorage-dependent and propagate as a monolayer attached to the cell culture vessel. (qiagen.com)
- Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (wikipedia.org)
- We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. (freepatentsonline.com)
- 18. A method according to claim 1, in which cell growth or cell viability is enhanced by at least 50% compared to growth in a cell culture medium without an elevated concentration of thymidine family member. (freepatentsonline.com)
- 19. A method according to claim 1, in which apoptosis of the animal cell in the cell culture medium is reduced to enhance cell viability. (freepatentsonline.com)
- Support non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins with Thermo Scientific™ Nunc™ Dishes with UpCell™ Surface. (fishersci.com)
- This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (hindawi.com)
- Out of nine temperatures tested between 4°C and 37°C, storage at 12°C, 16°C, and 20°C was optimal for maintenance of RPE cell viability, morphology, and phenotype. (hindawi.com)
- Based on previous publications on storage of cultured epithelial cells [ 20 - 23 ], we hypothesize that differences in storage temperature between 4°C and 37°C affect the viability, morphology, and phenotype of cultured RPE cells. (hindawi.com)
- I personally think that the massive interest in cultured meat brought a lot of interesting scientific ideas to market, but some of them lack a path for commercial viability. (foodnavigator.com)
- For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
- Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. (springer.com)
- She has extensive experience in a variety of cell culture techniques including isolation and culture of primary patient cells. (brighttalk.com)
- Mouse Müller Cell Isolation and Culture. (nih.gov)
- Later: Freezing and DNA isolation of part of the ES cell clones. (dkfz.de)
- This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. (nationwidechildrens.org)
- Finally, many standard techniques for analyzing proteins and protein distributions are more difficult to perform, because they require isolation of the cells from the matrix. (sciencemag.org)
- In addition, mouse ES cells have been used to generate in vitro cultures of neurons ( 26 ), skeletal muscle ( 27 ), and vascular endothelial cells ( 28 ). (pnas.org)
- The company's exVive3D™ human liver tissue model is constructed from three basic cellular elements: primary hepatocytes, primary endothelial cells, and primary stellate cells. (genengnews.com)
- Two key aspects of this process are initial endothelial cell (EC) dysfunction and subsequent phenotypic change and accumulation of smooth muscle cells (SMCs), with inherent disruption in local cell-cell communication. (strath.ac.uk)
- Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. (jci.org)
- In this study, we have found that lyso-PC increased steady state mRNA levels for two smooth muscle/fibroblast-directed growth factors, the A and B chains of PDGF and heparin-binding EGF-like protein (HB-EGF), in cultured human endothelial cells. (jci.org)
- Lyso-PC did not upregulate the expression of certain other inducible endothelial genes, including E-selectin, IL-8, or monocyte chemoattractant protein-1 in the same cells, in contrast to the coordinate pattern of activation typically observed with other stimuli, such as TNF alpha, bacterial endotoxin, or PMA. (jci.org)
- Endothelin-1 (ET-1) is a vasoconstrictive peptide of 21 amino acids that was first isolated from porcine vascular endothelial cells. (ahajournals.org)
- 8 11 12 In human endothelial cells, phorbol esters, activators of PKC, cause a marked and immediate stimulation of ET-1 production, accompanied by induction of preproendothelin mRNA. (ahajournals.org)
- This group has recently shown that epithelial cells extracted from the developing teeth of 6-month-old pigs continue to proliferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known as the 3T3-J2 cell line). (eurekalert.org)
- Suspension cells can survive and proliferate without being attached to a substratum. (qiagen.com)
- Primary cultures are formed from cells that survive the disaggregation process, attach to the cell culture vessel (or survive in suspension), and proliferate. (qiagen.com)
- These cultures will proliferate for a limited number of cell divisions, after which they will senesce. (qiagen.com)
- The cells will proliferate for an extended time, but usually the culture will eventually cease dividing, similar to senescent primary cells. (qiagen.com)
- An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
- 21. A method according to claim 1, in which the animal cell expresses a heterologous or recombinant protein. (freepatentsonline.com)
- A team of scientists led by Prof. Antonella Consiglio from the IDIBELL and the University of Barcelona, and Prof. Angel Raya from the Center of Regenerative Medicine of Barcelona have discovered that defective versions of human brain cells called astrocytes are linked to the buildup of a toxic protein that is one the hallmarks of Parkinson's disease. (news-medical.net)
- This group has been working on showing this goes on in human stem cells for at least 2 years, who knows how long it took them to find this out in mice, or narrow down this one specific protein. (slashdot.org)
- I would guess that the next step, maybe one they're already working on, is to show that induced pluripotent stem cells can be cultured on this same protein. (slashdot.org)
- Ellis also argues that cultured meat can bring benefits for food security, in terms of protein, and significant environmental sustainability benefits due to relatively lower greenhouse gas emissions, water use and land use, and less energy, compared to traditional meat production. (fao.org)
- 4 Since angiotensinogen protein, mRNA, and ACE have been identified in the PT cell, 5 6 7 8 renin had been, until recently, the only missing component for a complete RAS in the PT. (ahajournals.org)
- Naturally based" hydrogels such as Matrigel (made of native extracellular matrix proteins) and collagen (the major extracellular matrix protein) have been explored extensively for applications in three-dimensional tissue culture and regenerative medicine. (sciencemag.org)
- SCs cultured in insulin deprivation demonstrated a significant decrease on mRNA levels of p53, Bax, caspase-9, and caspase-3 followed by a significant increase of Bax and decrease of caspase-9 protein levels relatively to the control. (hindawi.com)
- The protein kinase C-activating phorbol ester phorbol myristate acetate stimulated ET-1 production in a concentration-dependent manner in cells of both rat strains, but this stimulation was significantly greater in SHR cells than in WKY cells. (ahajournals.org)
- Increased response of ET-1 production to protein kinase C activation appears to contribute in part to the observed enhancement of ET-1 production in SHR mesangial cells. (ahajournals.org)
- While cell lines have already been used safely to produce enzymes, oils and transgenic proteins, several concerns remain in how to keep cultured meat/protein products safe, according to the FDA and USDA. (foodengineeringmag.com)
- Delegates will leave the module with practical knowledge to propagate, maintain and characterize feeder and feeder-free stem cell lines. (ucl.ac.uk)
- Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells. (ucl.ac.uk)
- Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. (fishersci.com)
- The idea for the culturing technique originates from 1975, when Dr. J.G. Rheinwald and Dr. H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were gifted by Dr. Green). (eurekalert.org)
- culturing them required feeder cells which the article describes why that's bad, and 3. (slashdot.org)
- With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. (pnas.org)
- EBs from mouse pluripotent stem cells grown on feeder layers or in suspension may contain a variety of cell types. (pnas.org)
- 4. The method of claim 1 , wherein the human umbilical cord feeder cells consist of 100% human umbilical cord fibroblast feeder cells. (google.es)
- 7. The method of claim 6 , wherein the human umbilical cord feeder cells consists essentially of the human umbilical cord fibroblast feeder cells. (google.es)
- When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (atcc.org)
- Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (atcc.org)
- 2. A culture of cells grown by this technique or process. (thefreedictionary.com)
- Interestingly, spheroids derived from stem cells grown in 3D models show improved "stemness," that is, characteristics that may lead to increased efficacy in regenerative medicine. (genengnews.com)
- The process involves taking tissue from a live animal and extracting stem cells which are then grown into muscle fibres in a bioreactor. (fao.org)
- Marianne Ellis is a Biochemical Engineer at Bath University, UK and her team has taken cells grown and matured in a bioreactor and then combined them with other ingredients to make products such as burgers, sausages and meat balls. (fao.org)
- Studies were performed in both rabbit and rat renal PT cells grown in primary culture, as previously described. (ahajournals.org)
- Hematopoietic cells (derived from blood, spleen, or bone marrow) as well as some transformed cell lines and cells derived from malignant tumors can be grown in suspension. (qiagen.com)
- When cells have grown (by dividing) to cover the flasks' surface, the process of enzymic dissociation can be repeated and the cells replanted to additional flasks. (encyclopedia.com)
- Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (wikipedia.org)
- Skin cells have been grown in the laboratory since the mid 1970s, but until now there has not been a significant scientifically controlled study. (rxpgnews.com)
- The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
- It will also provide managers with insights into the intricacies of cell culture and the necessary laboratory infrastructure requirements. (ucl.ac.uk)
- The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor laboratory areas. (concordia.ca)
- In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Müller cells. (nih.gov)
- The idea of developing a laboratory instrument that handles and sorts individual, viable cells has been realized by researchers in the Laboratory for MEMS Applications in the Department of Microsystems Engineering at the University of Freiburg. (news-medical.net)
- In a search for alternative sources of meat and in response to concerns over meat consumption, animal welfare and greenhouse gas emissions, scientists have developed the ability to grow meat from animal cells in a laboratory. (fao.org)
- Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines. (routledge.com)
- Cell culture disposables for cell cultivation and cell monitoring in laboratory scale. (sartorius.com)
- With her book Culturing Life , Hannah Landecker contributes an important chapter to the recent history of these laboratory fractals. (jhu.edu)
- Cell culture describes the laboratory growth of cells derived from plants or animals. (encyclopedia.com)
- The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (encyclopedia.com)
- Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap offer a convenient solution to prepare laboratory samples. (fishersci.ca)
- 1998) Cells: a Laboratory Manual. (bio-medicine.org)
- The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
- The book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover and therefore delivers in a compact and clear way important theoretical as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. (powells.com)
- From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (corning.com)
- Rely on innovative vessels from Corning for quality and consistency in your cell culture applications. (corning.com)
- It is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. (sciencemag.org)
- Corning™ Untreated 245mm Square Low Profile BioAssay Dishes provide a superior option for applications where cell attachment is not desired. (fishersci.com)
- Using Nunc Glass Bottom Dishes in your research applications gives you the flexibility to convert your culture device into an imaging device. (fishersci.com)
- Gibco™ DMEM, Powder, High Glucose, No Sodium Bicarbonate Buffer is used in cell culture processing applications. (fishersci.com)
- Cell culture is a fundamental technique for a variety of applications. (brighttalk.com)
- And in therapeutics, 3D cell culture promises to realize several exciting applications, from patient-specific drug-efficacy screening to the manufacturing of bioprinted organs. (genengnews.com)
- GEN: Are 3D cell culture models as strongly focused as ever on drug safety testing, or are they finding new applications? (genengnews.com)
- We would argue that 3D cell culture models have been used for many years in basic research and disease modeling, notably in cancer research-this was, after all, one of the original applications of Corning® Matrigel®, a naturally occurring extracellular matrix for us in 3D cell culture. (genengnews.com)
- Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. (wiley-vch.de)
- Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (wiley-vch.de)
- Fortunately, bioprinters are becoming commonplace, and the applications that they enable-such as 3D cell cultures-are starting to go mainstream. (genengnews.com)
- Click here to register for free and to view the entire webinar: http://bit.ly/kosi_136 Since 2008 Kaiser Optical has worked closely with industry leading bioprocessing companies to develop a Raman solution enabled to deliver in situ, real-time measurement to a host of bioprocessing applications including but not limited to cell culture, fermentation, and purification. (environmental-expert.com)
- Since our first symposium in 2009, the 3D Cell Culture conference series has developed into a 'place to be' for experts active in this field, and will highlight successful applications and newest developments. (dechema.de)
- Success stories of 3D culture applications will be presented as well as newest technologies and innovations. (dechema.de)
- Relevant disease models, applications of 3D models in clinic and industry, predictive cell models for compound characterization and enabling technologies will be covered. (dechema.de)
- Optimization of cell culture conditions is necessary to ensure that cells are healthy and in optimal condition for downstream applications. (qiagen.com)
- Corning™ Stripette™ Individually Wrapped Plastic or Paper/Plastic Disposable Polystyrene Serological Pipets are ideal for sterile tissue culture applications, especially while wearing latex gloves, as the wrap reduces static cling. (fishersci.ca)
- These disposable, space saving containers are specifically designed for optimal plant cell culture applications. (mpbio.com)
- One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
- This Request for Applications (RFA), Papillomavirus In Vitro Cell Culture Systems, is related to the priority area of sexually transmitted diseases. (nih.gov)
- ELIGIBILITY REQUIREMENTS Applications may be submitted by domestic and foreign for-profit or nonprofit organizations, public and private, such as universities, medical colleges, hospitals, laboratories, units of State and local governments, and eligible agencies of the Federal government having adequate resources or access to resources to support papillomavirus in vitro cell culture research. (nih.gov)
- In the study being reported today, the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of the tooth (dental mesenchymal cells). (eurekalert.org)
- The scaffolds were then transferred into the abdominal cavities of rats, where conditions were favorable for the cells in the scaffolds to interact and develop. (eurekalert.org)
- The confluence of morphologic, genetic and biochemical approaches laid the foundations for study in this area, and they continue to strengthen our understanding of this essential aspect of cell structure and function. (nature.com)
- This test is intended for tissue samples that do not have any accompanying cytogenetic test order (e.g. chromosome analysis or microarray analysis) but needs to be cultured for other molecular genetic or biochemical testing (e.g. (nationwidechildrens.org)
- If tissue from products of conception (POC) is submitted and molecular genetic testing is requested on cultured cells, then submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (nationwidechildrens.org)
- cell cultivation via incubators, incubation and benchtop shakers and cell harvest via homogenisers and centrifuges with varoius volumes as well as PCR-based tests for mycoplasma contamination control. (sartorius.com)
- If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (nationwidechildrens.org)
- The limitations of cell culture include the finite doubling potential of most normal cells, the possibilities for unexpected infection with viruses or microorganisms, or even cross-contamination with other cell types. (encyclopedia.com)
- In the development of the portfolio we put a special focus on your needs for excellent cell performance and reliability as well as for advanced protection against contamination. (eppendorf.com)
- Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, and discover novel 3D cell culture systems, organ-on-chip, 3D imaging of organoids and other technologies in development. (smi-online.co.uk)
- 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many uses. (corning.com)
- Due to strict regulatory demands [ 18 , 19 ], the development of a suitable storage method will be essential to enable the transportation of viable cell constructs from centralized laboratories to operating theatres [ 18 ]. (hindawi.com)
- For this reason, it is exciting to consider the prospect of artificially growing enamel, or even whole teeth, using culturing and transplantation techniques. (eurekalert.org)
- With the demonstration of long-term survival of RPE cell transplants both in various animal models and in humans [ 8 - 13 ], transplantation offers the prospect of a single intervention cure. (hindawi.com)
- The transplantation of RPE grafts enables appropriate implantation and orientation of an organized RPE cell layer in the retina [ 5 , 14 , 15 ] and circumvents several of the complications associated with the use of RPE cell suspensions [ 6 , 8 , 15 , 16 ]. (hindawi.com)
- Autologous tissue for transplantation may also come from organoids produced via 3D cell culture. (genengnews.com)
- The cover can be snapped on tight, for long-term culture, or loosely fitted during acclimation to external conditions prior to transplantation. (mpbio.com)
- Today, during the 85thth General Session of the International Association for Dental Research, a team of researchers from the Institute of Medical Science, the University of Tokyo (Japan), reports on a new technique for culturing cells that have the capacity to produce enamel. (eurekalert.org)
- Researchers have seen that spheroids display enhanced anti-inflammatory, tissue regenerative, and reparative responses, as well as better post-transplant survival of mesenchymal stem cells. (genengnews.com)
- UPM launches GrowDex-T - a new hydrogel offering researchers exceptional imaging properties for visualization of cells in 3D. (news-medical.net)
- Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study. (springer.com)
- With the increasing incidence of neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, and Parkinson's disease, researchers are testing 3D cellular models that could be superior to animal studies because the cells are derived from humans instead of rodents. (genengnews.com)
- Researchers are now bridging the gap between natural and synthetic gels by combining well-characterized synthetic materials with biomimetic cues to support physiologically relevant cell-gel interactions (see the figure). (sciencemag.org)
- Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (springer.com)
- Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (springer.com)
- New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. (wiley-vch.de)
- The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. (springer.com)
- It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. (qiagen.com)
- A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. (google.it)
- They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. (google.it)
- A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. (worldcat.org)
- Plant cell, tissue and organ culture. (worldcat.org)
- Plant Cell, Tissue and Organ Culture - incl. (springer.com)
- Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. (springer.com)
- Primary cells, finite cultures, and continuous cell lines differ in their proliferative potential (see below). (qiagen.com)
- Finite cell cultures are formed after the first subculturing (passaging) of a primary cell culture. (qiagen.com)
- The phenotype of these cultures is intermediate between finite cultures and continuous cultures. (qiagen.com)
- Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. (qiagen.com)
- To maximize cell yield, choose from our comprehensive line of standard and custom culture media, including basal salt solutions, antibiotics, sera, and specialty media. (corning.com)
- The system consists of interconnected cell culture chambers and a peristaltic pump to create a continuous flow of media over cells. (sciencemag.org)
- media without fixing the cells. (bio.net)
- Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). (brighttalk.com)
- Sartolab ® BT with polyethersulfone (PES) is designed for cell culture media and other solutions. (sartorius.com)
- Of key importance were specifically designed flasks and appropriate nutrient media, as well as the meticulous handling ("washing") of cells. (jhu.edu)
- cellTRAY forms the basis for lab-on-a-chip technology as it provides the means to automatically dispense and treat cells continually with reagents and cell culture media. (nsti.org)
- What kinds of substances used in cell culture media would be present in meaningful amounts in the finished food product, and are ordinary food ingredient evaluation procedures sufficient? (foodengineeringmag.com)
- Topics covered include aseptic technique, media preparation, cell culture evaluation as well as maintenance and storage of cell lines. (triton.edu)
- 3D cell culture, besides being more convenient than animal models (and relatively free of ethical issues), permits extraordinary control-from the genetic level upward. (genengnews.com)
- the genetic manipulation of stem cells to create mini-brains, and the development of tools and imaging technologies for high-content phenotypic screening and validation. (genengnews.com)
- This will provide novel mechanistic insights into cardiovascular disease and a well-characterised in vitro model of vascular cell-cell communication suitable for upscaling into an array format for multiplexed, label-free drug screening. (strath.ac.uk)
- The plan is to release hybrid products combining plant-based and cell based meat in 2021 and a second line of 100% cultured ground meat products suitable for things like burgers and nuggets at a cost of " less than $10 per pound " by 2022 (current small-scale production costs are c. $150/lb for chicken and $200/lb for beef). (foodnavigator.com)
- Preferably, the cell culture medium comprises a semi-solid medium, which is a serum free or chemically defined medium. (freepatentsonline.com)
- 7. A method according to claim 1, in which the cell culture medium is substantially free of serum. (freepatentsonline.com)
- Used for culture of variety of cell types under low serum conditions. (fishersci.com)
- In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
- Tissue-level effects can now be probed in vitro thanks to recently developed systems of three-dimensional (3D), multicellular, "organotypic" cell cultures, which mimic functional responses of living tissue. (osti.gov)
- 20. A method according to claim 1, in which the animal cell is transformed with an expression vector, the expression vector being a member of a nucleic acid library. (freepatentsonline.com)
- A more comprehensive reference on animal cell culture can be found in Culture of Animal Cells: A Manual of Basic Technique, 5th edition, by R. Ian Freshney (24). (atcc.org)
- Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses. (routledge.com)
- A central advantage of the cell culture technique is its simplicity compared to the difficulties of studies using whole plant or animal organs, which are usually composed of many different cell types. (encyclopedia.com)
- 1. Freshney, R.I. (1993) Culture of Animal Cells: a Manual of Basic Technique. (bio-medicine.org)
- The Food and Drug Administration (FDA) held a public meeting July 12 on " Foods Produced Using Animal Cell Culture Technology " to give the public an opportunity to provide comments on federal regulation of the future production of foods using animal cell culture technology. (lexology.com)
- How likely is it that cultured animal cells could produce harmful substances as a result of errors in the culture process? (foodengineeringmag.com)
- What departures from these characteristics would be expected in food products of animal cell culture technology derived from their respective sources? (foodengineeringmag.com)
- In summary, these results confirm the presence of renin mRNA in cultured proximal tubular cells and suggest that a low-level, constitutive secretion of renin occurs in this system that is decreased by A23187. (ahajournals.org)
- Renin secretion from JG cells has been well described: Renin release from these cells is largely regulated by intracellular calcium and cAMP, such that a decline in intracellular calcium or an increase in cAMP stimulates renin secretion in JG cells. (ahajournals.org)
- 3 4 5 Previous studies demonstrated constitutive expression of ET-1 transcripts and peptide secretion in cultured glomerular mesangial cells. (ahajournals.org)