The effect of chelating agents on iron mobilization in Chang cell cultures. (1/155046)

The investigation of chelating agents with potential therapeutic value in patients with transfusional iron overload has been facilitated by the use of Chang cell cultures. These cells have been incubated with [59Fe]transferrin for 22 hr, following which most of the intracellular radioiron is found in the cytosol, distributed between a ferritin and a nonferritin form. Iron release from the cells depends on transferrin saturation in the medium, but when transferrin is 100% saturated, which normally does not allow iron release, desferrioxamine, 2,3-dihydroxybenzoic acid, rhodotorulic acid, cholythydroxamic acid, and tropolone all promote the mobilization of ferritin iron and its release from cells. They are effective to an approximately equal degree. The incubation of [59Fe]transferrin with tropolone in vitro at a molar ratio of 1:500 results in the transfer of most of the labeled iron to the chelator, reflecting the exceptionally high binding constant of this compound. How far these phenomena relate to therapeutic potentially remains to be seen.  (+info)

In vitro effects of 2,4-dichlorophenoxy acetic acid (2,4-D) on bovine cells. (2/155046)

Bovine fetal muscle cells were exposed to culture media containing 2 mg and 20 mg per liter of 2,4-dichlorophenoxy acetic acid (2,4-D) for varying intervals to determine the in vitro response of mammalian cells to this compound. The concentrations of 2,4-D used were comparable to those used in spray programmes although the residues normally found in pasture are much lower since 2,4-D is rapidly degraded under field conditions. Untreated and treated cultures were analyzed for total cell count, mitotic index and the percentages of differentiating and degenerating cells. The response of cultures to treatment was similar irrespective of the concentrations of 2,4-D used although in higher concentrations there was an initial drop in mitotic index. Other changes noted in treated cultures included an increase in differentiating and degenerating cells compared to those in control. The mitotic cells in treated cultures exhibited unipolar and tripolar spindles and a variety of other abnormalities including malorientation of the mitotic apparatus in relation to the axis of the cell. Myoblasts in initial stages of myogenesis were noted to be in mitosis in treated cultures suggesting that 2,4-D may have a stimulatory effect on myoblasts which in normal myogenesis are in post mitotic stage.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (3/155046)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Glycopeptides from the surgace of human neuroblastoma cells. (4/155046)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (5/155046)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (6/155046)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (7/155046)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (8/155046)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Cell Culture ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... A 3D cell culture model showed cell growth similar to that of in vivo than did a monolayer culture, and all three cultures were ...
Spinner type vessels are used for mammalian or plant cell culture. They are adequate for cell suspensions and attachment ... usually a mixture of cells, medium and products like proteins that can be harvested). The vessels are usually made out of glass ... dependent cell types. v t e (Articles lacking sources from December 2009, All articles lacking sources, Laboratory equipment, ...
The Sf9 cell line is one of the most commonly used lines in insect cell culture. Drugmand, Jean-Christophe; Schneider, Yves- ... v t e (Cell culture techniques, Biopharmaceuticals, All stub articles, Pharmacology stubs). ... Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are ... The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are ...
... and cell loading would need to be added for the cell culture maintenance. Perfusion in microfluidic cell culture is important ... Gel free 3D cell cultures have been developed to allow cells to grow in either a cell dense environment or an ECM poor ... Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... These reduced cell numbers make studying non-dividing or slow dividing cells (e.g., stem cells) easier than traditional culture ...
Cell culture Cell lines Cell culture assay Hydrogel Madin-Darby Canine Kidney cell line Microphysiometry Fey S, Wrzesinski K ( ... cell-cell/cell-matrix interaction, and/or cell migration, but the available assays are not optimized for 3D cell culturing. ... Lastly, 3D cell cultures have greater stability and longer lifespans than cell cultures in 2D. This means that they are more ... Furthermore, 3D cell cultures provide more accurate depiction of cell polarization, since in 2D, the cells can only be ...
... through the inhibition of cell metabolic pathways. Cell culture evaluations are the precursor to whole animal studies and are a ... A cell culture assay is any method used to assess the cytotoxicity of a material. This refers to the in vitro assessment of a ... Cell culture assays are standardized by ASTM, ISO, and BSI (British Standards Institution.) Biology portal Technology portal ... Löw-Friedrich, I; von Bredow, F; Schoeppe, W (1991). "A cell culture assay for the detection of cardiotoxicity". Journal of ...
... is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture ... More specific terms include organotypic culture, tissue slices and explants. Neuronal primary cell cultures are cells collected ... primary cell cultures did not become widespread until the 2000s. These cultures present several advantages over cell lines, ... In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized ...
The cell culture bioreactor-known commercially as the Rotary Cell Culture System (RCCS) -- boasts several advantages that ... Lab-grown cell cultures tend to be small, flat and two-dimensional, unlike normal cultures. But tissues grown in the RCCS are ... The Rotary Cell Culture System (RCCS) is a device designed to grow three-dimensional cell clusters in microgravity. In the ... A closed tubular cylinder forms the system's cell culture chamber, which is filled with a liquid medium in which cells grow on ...
Another type of material that is used for open-microfluidic cell culturing is paper-based microfluidics. Cell culturing on ... such as quorum sensing or co-culturing of several types of cells. A majority of cell culturing has been carried out by ... The adsorption of small biological molecules from cell culturing samples as well as the release of oligomers into the culture ... where the device is open for the introduction and culturing of cells, and can be sealed prior to analysis. Cells and proteins ...
Air liquid interface cell culture (ALI) is a method of cell culture by which basal stem cells are grown with their basal ... This method of cell culture aims to be used to study fundamental aspects of the respiratory epithelium, such as cell-to-cell ... The cell culture method used involves the isolation of cells into culturing with growth factors to grow over time. Once the ... Air-liquid interface cell culture compares to standard cell culture practices by specifically aiming to restore the ...
3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high- ... Because of the ability of 3D cell culturing by magnetic levitation to bring cells together, co-culturing different cell types ... Standard monolayer cell culturing on tissue culture plastic has notably improved our understanding of basic cell biology, but ... Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio- ...
The European Collection of Authenticated Cell Cultures houses and supplies cell lines. It is part of the Culture Collections of ... ECACC is one of the first collections of authenticated cell cultures worldwide and now holds cell lines from 45 species ... the culture collections website alongside a range of services such as Mycoplasma testing and training courses for cell culture ... Culture Collections Official home page v t e (Use dmy dates from April 2022, Articles with topics of unclear notability from ...
Different cell lines and cell types have been cultured in NFC, including e.g. differentiation of human hepatic cells to ... like cell culturing. GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. NFC fiber network structure ... functional organotypic cultures, and proliferation of human pluripotent stem cells. Organotypic liver cell cultures can be used ... Additionally, 3D cell culture using wood-based nanocellulose hydrogel can be used for tissue engineering. Ahmed, Enas M. (2015- ...
Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing ... While SILAC had been mostly used in studying eukaryotic cells and cell cultures, it had been recently employed in bacteria and ... Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects ... Amanchy R, Kalume DE, Pandey A (January 2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying ...
Dawn news election cells. Dawn news election cells. Retrieved 21 February 2015.- Hoodbhoy, Pervez. "Noam Chomsky interviewed by ... Pakistani culture, Pakistani social culture, Himalayan culture, South Asian culture). ... The Culture of Pakistan (Urdu: پاکستانی ثقافت Pākistāni S̱aqāfat) is based in the Indo-Persian cultural matrix that constitutes ... The consumption of tea in Pakistan, called chai (چائے), in Urdu, is of central significance to Pakistani culture. It is one of ...
doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. Although the majority of Afanasievo burials ... Chalcolithic cultures of Asia, Bronze Age cultures of Asia, Archaeological cultures of China, Archaeological cultures in ... In particular, contacts between the Afanasievo culture and the Majiayao culture and the Qijia culture are considered for the ... The Afanasievo culture, or Afanasevo culture (Afanasevan culture) (Russian: Афанасьевская культура Afanas'yevskaya kul'tura), ...
Cell. 183 (4): 890-904. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. (Articles with short ... Nearby Saka cultures were the Tagar Culture of the Minusinsk Basin, as well as the Pazyryk Culture (ca. 500-200 BCE) in the ... 93.631836 The Sagly-Bazhy culture or Sagly/Uyuk culture, also known as Chandman culture in Mongolia (Ulaangom cemetery), refers ... It is the last stage of the Uyuk culture. This period of Scythian culture covers a period from the 5th century BCE to the 2nd ...
Cell. 183 (4): 890-904. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. S2CID 214725595. Zhang, Fan ... Dental analysis has shown that the Chemurchek culture consumed ruminant dairy products. The people of the Chemurchek culture ... The Chemurchek culture had various characteristics of West European origin. Chemurchek funerary and ritual structures ... The mortuary position of the deceased (supine position with flexed legs) is similar to that of the Afanasievo culture. ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Lee 2023, ""SlabGrave" was ... Archaeological cultures of East Asia, Archaeological cultures of Northern Asia, Bronze Age cultures of Asia, Iron Age cultures ... The Ulaanzuukh culture (c. 1450-1150 BCE) is an archaeological culture of the Late Bronze Age eastern Mongolia. It likely ... Indeed, the genetic profile of individuals belonging to this culture is genetically identical to the Slab Grave culture ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Antonio Sagona. Rethinking the Kura- ... mun INDUS VALLEY CIVILIZATION Corded Ware Culture Yamnaya Culture Kura- Araxes Afanasievo culture Botai culture Sarazm culture ... The Kura-Araxes culture (also named Kur-Araz culture, Mtkvari-Araxes culture, Early Transcaucasian culture) was an ... Kura-Araxes culture, Bronze Age cultures of Asia, Chalcolithic cultures of Asia, Archaeological cultures of West Asia, ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. Meakin, Annette M. B ... Other post-Xiongnu cultures, such as the Kokel Culture have also been recorded nearby. Tashtyk culture mask Tashtyk culture ... The Tashtyk culture was Late Iron Age archaeological culture that flourished in the Yenisei valley in Siberia from the 1st ... it was preceded by the Tagar culture. The Tashtyk culture was first surveyed by the Russian archaeologist Sergei Teploukhov. ...
Marchi, Nina (2022). "The genomic origins of the world's first farmers". Cell. 185 (11): 1842-1859. doi:10.1016/j.cell.2022.04. ... Archaeological cultures of Europe, Neolithic cultures of Europe, Archaeological cultures in Austria, Archaeological cultures in ... Archaeological cultures in France, Archaeological cultures in Germany, Archaeological cultures in Hungary, Archaeological ... cultures in Moldova, Archaeological cultures in the Netherlands, Archaeological cultures in Poland, Archaeological cultures in ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. hdl:21.11116/0000-0007-77BF-D. ISSN 0092-8674. Damgaard, P. B.; et ... In 2009, a genetic study of ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and the ... The Tagar culture was a Bronze Age archeological culture which flourished between the 8th and 1st centuries BC in South Siberia ... The Tagar culture was preceded by the Karasuk culture. They are usually considered a mixed population descended from peoples of ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Török, Tibor (July 2023). " ... Karasuk culture (Ural to Altai) Aldy-Bel culture Okunevo culture Pazyryk culture The origins of the Karasuk culture are complex ... 2009) published a genetic study of ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and ... Bronze Age cultures of Asia, Archaeological cultures of Siberia, Iranian archaeological cultures, Archaeological cultures in ...
Cell. 183 (4): 890-904.e29. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. PMC 7664836. PMID 33157037. Rogers, Leland Liu; ... various Saka cultures such as the Tagar culture, the Pazyryk culture and the Aldy-Bel culture for a period of several centuries ... Archaeological cultures of East Asia, Archaeological cultures of Northern Asia, Bronze Age cultures of Asia, Iron Age cultures ... The Slab-Grave culture is an archaeological culture of Late Bronze Age and Early Iron Age Mongolia. The Slab-Grave culture ...
Cell. 184 (18): 4612-4625.e14. doi:10.1016/j.cell.2021.07.013. ISSN 0092-8674. PMC 8445022. PMID 34352227. Broushaki, F; Thomas ... Epipalaeolithic cultures, Archaeological cultures in Israel, Archaeological cultures in Jordan, Archaeological cultures in ... Natufian culture, Industries (archaeology), Archaeological cultures of West Asia, Archaeological cultures of the Near East, ... The Natufian culture (/nəˈtuːfiən/) is a Late Epipaleolithic archaeological culture of the Neolithic prehistoric Levant in ...
Cell. 163 (3): 571-582. doi:10.1016/j.cell.2015.10.009. PMC 4644222. PMID 26496604., S14-15. This sample is marked "RISE505". ... MIDDLE KINGDOM Kerma culture Longshan Culture Qijia culture Baodun culture Shijiahe culture Abashevo Culture Catacomb Culture ... Andronovo culture Sintashta culture BMAC Vakhsh Tarim mummies Okunev culture Karakol culture Samus culture Ulaanzuukh culture ... 2009) published a study of the ancient Siberian cultures, the Andronovo culture, the Karasuk culture, the Tagar culture and the ...
Sagly culture Jeong, Choongwon (12 November 2020). "A Dynamic 6,000-Year Genetic History of Eurasia's Eastern Steppe". Cell. ... The Chandman culture, also Chandmani culture, was a nomadic culture that existed in northwestern Mongolia and southern Siberia ... The Chandman culture has been linked to the nearby cultures at Sagly and Uyuk. The Chandman population seems to have been in ... It is associated with the Scythian/Saka horizon, and is part of the more general Saka Uyuk culture. The Chandman culture was ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. Török, Tibor (July 2023). " ... MIDDLE KINGDOM Kerma culture Longshan Culture Qijia culture Baodun culture Shijiahe culture Abashevo Culture Catacomb Culture ... Andronovo culture Sintashta culture BMAC Vakhsh Tarim mummies Okunev culture Karakol culture Samus culture Ulaanzuukh culture ... Sintashta material culture also shows the influence of the late Abashevo culture, derived from the Fatyanovo-Balanovo culture, ...
Cell. 183 (4): 890-904, Figure 1 A, B, C. doi:10.1016/j.cell.2020.10.015. ISSN 0092-8674. "Okunev Culture". The Great Soviet ... Okunev culture shares some elements of its material culture, including pottery. with a number of local contemporaneous cultures ... Okunev culture'), sometimes also Okunevo culture, was a south Siberian archaeological culture of pastoralists of the early ... The Okunev people of Tuva show stronger influence from the Pits culture and early Catacomb culture of Ukraine. He argues that ...
Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Cell Culture ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... A 3D cell culture model showed cell growth similar to that of in vivo than did a monolayer culture, and all three cultures were ...
Authority to move uncleared biosecurity risk goods for Microorganisms/Cell Cultures/GMOs, Invertebrates, Vertebrate Lab ... Authority to move uncleared biosecurity risk goods for Microorganisms/Cell Cultures/GMOs, Invertebrates, Vertebrate Lab ... Forms and templates for importing cell cultures. Forms and templates you may need when importing cell cultures. ...
Compacted cell culture media, like the custom EZMix™ product line, delivers equivalent performance to dry powder media and ... Features and benefits of compacted cell culture media: *Accelerated dissolution streamlines media preperation ... Sterile Filtration Good cell culture practices start with proven products like Stericup® and Millex® filters. ... a technology to produce compacted cell culture media. This dry granulation technique applies compression force and milling to ...
The cells showed an apparent increase in size, diffuse cell rounding, and a refringent aspect (Figure 1B). In Hep-2C cells, the ... Infection of Cultured Human and Monkey Cell Lines with Extract of Penaeid Shrimp Infected with Taura Syndrome Virus On This ... refringent cells. Some cells remained isolated but with altered morphology (Figure 1F). RD, Hep-2C, and BGM cells injected with ... Infection of Cultured Human and Monkey Cell Lines with Extract of Penaeid Shrimp Infected with Taura Syndrome Virus. Emerging ...
The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily ... The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily ... The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily ... Neuronal and non-neuronal catechol-O-methyltransferase in primary cultures of rat brain cells. *Mark ...
In both infected A549 cells and primary human hepatocytes, silvestrol has the potential to exert a significant inhibition of ... Therefore, we investigated the effects of silvestrol on ZIKV replication in A549 cells and primary human hepatocytes. Two ... Cell Culture. Human A549 cells were cultured in DMEM supplemented with 4.5 g/L glucose, 10% fectal calf serum (FCS), 2 mM l- ... For this, Vero cells were seeded in a 6-well plate (3 × 105 cells/well) and infected 6 h later with cell culture supernatant in ...
Copyright © 2024 NovaHep AB normal human cell culture. Theme: Creative Blog by Bishal Napit. Powered by WordPress. ... Twin Results of Non-Coding RNAs (ncRNAs) in Most cancers Stem Cell Biology The identification of most cancers stem cells (CSCs ... Contributions of Electron Microscopy to the Structural Cell Biology * Self-Organizing Human Induced Pluripotent Stem Cell ... Contributions of Electron Microscopy to the Structural Cell Biology * Self-Organizing Human Induced Pluripotent Stem Cell ...
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Anchorage-dependent culture of line 10 guinea-pig hepatoma cells. S. J. Kleinschuster, H. J. Rapp, A. V. Johnston, K. R. Van ... Anchorage-dependent culture of line 10 guinea-pig hepatoma cells. / Kleinschuster, S. J.; Rapp, H. J.; Johnston, A. V. et al. ... Anchorage-dependent culture of line 10 guinea-pig hepatoma cells. In: Experientia. 1980 ; Vol. 36, No. 10. pp. 1239-1240. ... title = "Anchorage-dependent culture of line 10 guinea-pig hepatoma cells",. abstract = "A simple method for the anchorage- ...
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... through cell sorting) obtained 36 h after surgery. Total BMC and cells after depletion of CD4+ DCs were used to set up cultures ... CD11chi cells were gated among SSClo/FSClo cells. Dot plots show the expression of CD4 and MHC class II on gated CD11chi cells ... Cell Sorting. Bone marrow cells were adjusted to a density of 1 × 107 cells/ml in PBS supplemented with 2% FCS and were stained ... An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J Immunol ...
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The Tissue Culture Facility (TCF) is a supply center and provides UNC Lineberger members and UNC colleagues with a wide variety ... cell culture flasks and dishes, and many other in vitro cell culture and molecular biology-related items. ... American Type Culture Collection, NIGMS Human Genetic Mutant Cell Repository). Cell lines include normal and transformed ... Tissue Culture Facility The Tissue Culture Facility (TCF) is UNC Linebergers most comprehensive and widely used core facility ...
Expanding Role of Dopaminergic Inhibition in Hypercapnic Responses of Cultured Rat Carotid Body Cells: Involvement of Type II ... In functional CB-petrosal neuron cocultures, sulpiride potentiated hypercapnia-induced Δ[Ca2+]i in type I cells, type II cells ... i in type I cells. However, these stimuli sometimes triggered a secondary, delayed Δ[Ca2+]i in nearby type II cells, ... Here, we tested the hypothesis that DA may also inhibit type II cell function. When applied alone, DA (10 μM) had negligible ...
Cell culture and transfection. Request a detailed protocol HEK293 and NIH3T3 cells were cultured in DMEM (HyClone, South Logan ... 2012) DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human Cancer cells Journal of ... All cell lines were authenticated using STR profiling method and were tested negative for mycoplasma contamination. All cells ... 2003) Conserved microtubule-actin interactions in cell movement and morphogenesis Nature Cell Biology 5:599-609. ...
... ... Walter, Emmanuel B. and Hills, Susan L. "Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived ... Walter, Emmanuel B. and Hills, Susan L. "Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- derived ... Walter, Emmanuel B. and Hills, Susan L. (2018). Japanese Encephalitis (JE) vaccine = GRADE for inactivated Vero cell culture- ...
Authorities also found nine cell phone chips and one charger."Each one had a cellular with a number of chips," ... Juan Ramon Arevalo, director of the prison known as Zacatras, said the gang members had introduced the cell phones, wrapped in ... but you really want a cell phone and you want it real bad. What to do? Maybe this: Capt. ... Main Blog , Inmates Hid Cell Phones (and Charger!) Insider Their Body. Inmates Hid Cell Phones (and Charger!) Insider Their ...
Our PC-12 (High differentiation) Cells Complete Medium price is reasonable. Check more details about PC-12 (High ... and high-quality products for cell research and cell culture experiments. ... Cells which could promote cell proliferation and maintain cell properties for this cell. ... Cells Complete Medium is a ready-to-use medium that can be directly used for the culture of PC-12 (High differentiation) Cells ...
Fabrication of Ultra-Thin Micro-Porous PDMS Membrane for Cell Co-Culture in Blood Brain Barrier Model on Chip. By Riz Gamuz on ... Home » Fabrication of Ultra-Thin Micro-Porous PDMS Membrane for Cell Co-Culture in Blood Brain Barrier Model on Chip ...
Cell and tissue culture technologies have an increasing importance in the fields of basic and applied life science. New culture ... Cell and tissue culture technologies have an increasing importance in the fields of basic and applied life science. New culture ... Cell culture plates are made of high clarity, 100% virgin polystyrene.. • Vacuum Plasma tissue culture treatment.. • Clear lot ... Permeable supports may effectively improve the culture of polar cells, because these supports allow cells to secrete on and ...
Cells with dendritiform morphology first appeared in cultures by 2-3 wk. The majority of cells produced were large cells which ... Cells with dendritiform morphology first appeared in cultures by 2-3 wk. The majority of cells produced were large cells which ... Cells with dendritiform morphology first appeared in cultures by 2-3 wk. The majority of cells produced were large cells which ... Cells with dendritiform morphology first appeared in cultures by 2-3 wk. The majority of cells produced were large cells which ...
Cell Culture. Chapter 11. DNA Isolation. Chapter 12. PCR and Purification of Amplicon ...
Cell culture and transfection studies. N18-TG2 cells were cultured in DMEM supplemented with 10% fetal calf serum (FCS), 60 nm ... NCB-20 and NG108 cells were cultured in DMEM supplemented with 10% FCS, HeLa cells were cultured in DMEM supplemented with 10% ... HeLa cell extract was used instead of HEK-293S cells. Similar to HEK-293S cells, HeLa cells do not support 5HT3R reporter ... calf serum, and HEK-293 cells were cultured in DMEM supplemented with 5% FCS, 5% bovine serum, and antibiotics. Cultured cells ...
  • Historically, treatment of this condition has required donor corneal tissue coupled with invasive surgery, but recently a new therapeutic option has surfaced: cultured corneal endothelial cells. (
  • A group of researchers in Japan investigated whether cultured human corneal endothelial cells injected into the anterior chamber could restore endothelial function and corneal transparency. (
  • [ 1 ] Eleven patients (mean age, 64.4 years) diagnosed with pseudophakic bullous keratopathy who had no detectable corneal endothelial cells were enrolled. (
  • The degenerated endothelial cells were surgically removed, and then cultured human endothelial cells were injected into the anterior chamber. (
  • The promise of cultured corneal endothelial cells does not come without concerns, such as the fate of those cells that do not attach to the cornea, because of the risk for ectopic tumor formation. (
  • The authors hypothesized that the cultured cells were primarily responsible for the restored endothelial function, but acknowledged that the action of the ROCK inhibitor could have also prompted the recipient's own endothelial cells to replicate. (
  • The safety of cultured endothelial cells with ROCK inhibitors must be confirmed, but this proof-of-concept study should be considered a success. (
  • Identification and characterization of cell-substratum adhesion receptors on cultured human endothelial cells. (
  • A series of immunological approaches was utilized to identify the molecules involved in cell-substratum adhesion of human endothelial cells (EC) derived from adult large vessels, fat capillaries, and umbilical veins. (
  • For example, they are well versed in isolating endothelial cells and epithelial cells from animals (wild-type and knock-out murine strains, rat strains) from any desired tissue. (
  • Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (
  • Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. (
  • Bob Stevenson came to me one day, I knew him personally, of course, from former meetings on tissue culture subjects, and Bob said, 'Len, look, we know that you're struggling to keep up with the demand for these cells, and it's apparent to you and to me that the NIH and our grantees are dependent on you as a source of these cells for their work. (
  • We assessed the genomic fidelity of paired early- and late-passage hESC lines in the course of tissue culture. (
  • o Large quantity production of non-hybridoma cell secreted proteins. (
  • To directly test the importance of these molecules in cell-substratum adhesion, these proteins were purified by a combination of ion exchange, lectin affinity, and immunoaffinity chromatography and used to block the biological activity of the adhesion-disrupting polyclonal antibody. (
  • The quality and amount of therapeutic proteins produced by cultured mammalian cells rely heavily on the culture environment. (
  • The feeder cells secrete growth factors (proteins that stimulate cell growth and differentiation - typically the most expensive part of any growth media) that are then fed into the central tank so that Integriculture doesn't have to buy in growth factors from third parties (which are typically produced via microbial fermentation - ie. (
  • THE NATIONAL CELL CULTURE CENTER NIH GUIDE, Vol, 21, No. 21, June 5, 1992 P.T. 34 Keywords: Biological Resources Cell Lines National Center for Research Resources The National Cell Culture Center is a resource facility that provides large-scale mammalian cell culture services. (
  • This paper describes a method for evaluating gene expression changes for cultured NS0 cells, a mouse-derived myeloma cell line, under culture environment conditions, such as ammonia buildup, known to affect product quality. (
  • Oesterreich and Kaipparettu in cooperation with others in their laboratory found that chicken eggs whites enabled them to grow both normal and tumors cells in three-dimensions. (
  • An interdisciplinary team from NCI, NIBIB, and CIT SPIS laboratories has developed microfabricated synthetic mimetics of vasculature to provide oxygen to 3D cultured tumors and other tissues. (
  • Another important driver of the cell culture market will be the production of seasonal influenza vaccines, as well as pandemic vaccine candidates. (
  • Cell-based vaccine production dramatically reduces the possibility for contamination and promises to be a more reliable, flexible, and expandable platform than egg-based methods. (
  • Cell-based technology offers many benefits over egg-based production, including scalability, production risk mitigation (In the case of an avian flu pandemic, egg-producing flocks could decline, jeopardizing vaccine production capabilities. (
  • Leonard Hayflick (b. 1928), the recipient of several research prizes and awards, including the 1991 Sandoz Prize for Gerontological Research, is known for his research in cell biology, virus vaccine development, and mycoplasmology. (
  • He also made the first oral polio vaccine produced in a continuously propogated cell strain - work which contributed to significant virus vaccine development. (
  • The researchers plan to use the same viral delivery system to genetically modify the cells and assess how impairing cell fusion alters myotube growth. (
  • However, because TSV could potentially represent a public health threat, we explored whether this viral agent might be capable of infecting cultured mammalian cells. (
  • RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (
  • Rockland Inc. is making available its expertise for production of CAR-modified T and other immune cells using both viral and non-viral transfection methodologies. (
  • Specifically, the Cell Culture Center supports basic research by providing investigators with the following customized services: o Large quantity production of mammalian cells in suspension or monolayer cultures. (
  • Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (
  • Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (
  • In Hep-2C cells, the cellular monolayer was partially destroyed. (
  • The generation of 3D tumor spheroids in vitro is a useful model to examine malignant cells and tumorigenesis. (
  • Recently, the first in vitro norovirus cell culture model was reported for a virus that infects mice ( 18 , 19 ). (
  • Studies evaluating potential chemotherapeutics and multi-drug resistance in cancer cells have been established using in vitro 2D culture and in vivo animal models. (
  • The ability of astrocytes to communicate with surrounding cells through gap-junctional coupling or signaling via the release of transmitters makes characterization of these cells difficult in vitro and even more so in vivo . (
  • To simplify the complexity of common in vitro systems, introduced by intercellular communication between astrocytes, we developed a novel cell culturing method, in which purified rat visual cortical astrocytes were grown in spatially defined cell-adhesion wells which we termed micropits. (
  • Hayflick is known for discovering that human cells divide for a limited number of times in vitro (refuting the contention by Alexis Carrel that normal body cells are immortal), which is known as the Hayflick limit, as well as developing the first normal human diploid cell strains for studies on human ageing and for research use throughout the world. (
  • We established a novel, human embryonic stem cell (hESC) in vitro model to examine the radiobiological effects in human cells. (
  • While cells naturally reside in a highly crowded tissue context, in culture they become drowned in a sea of media, and rapidly lose their function and therapeutic potential. (
  • Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. (
  • The use of a three-dimensional cell culture systems has become so important that the National Cancer Institute has launched a new Tumor Microenvironment Network focusing on studies of the cellular microenvironment - relying heavily on three-dimensional culture systems and encouraging initiatives to improve techniques. (
  • 3D cell cultures better simulate the in vivo environment for tumor growth, but is currently limited by insufficient mass transport of oxygen due to a lack of vasculature. (
  • Pimonidazole staining of a tumor organoid (OVCAR8-dsRed2 cells) removed from a micropillar membrane after a 7 d culture in the bioreactor system. (
  • Using an improved cell blot technique, which permits sensitive and rapid comparison of PrP(Sc) levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. (
  • Transmembrane transfectors (carriers) are increasingly being viewed as helpful or even necessary to improve cellular delivery in connection with antisense tumor targeting and other applications requiring cell membrane transport of DNAs, RNAs, and other oligomers. (
  • The results of recent investigations suggest that radiolabeled DNAs and other oligomers for antisense targeting of tumor cells require transmembrane transfectors (carriers) for improved cell membrane transport. (
  • From stem cell culture to cancer cell biology to drug screening, 3D cell culture methods are providing more in vivo -like results across numerous cell culture applications. (
  • In cell biology research, cell screening technology has proved to be important in finding high-producing cell lines, and research is now focused on how to predict growth characteristics of cells at an earlier stage. (
  • We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. (
  • Whether you're just getting started in 3D cell culture, looking for proven ways to scale up, or moving to high throughput screening, Corning can help you break through the barriers to creating more in vivo -like environments and predictive models. (
  • Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (
  • The design of the RWV bioreactor is based on the principle that organs and tissues function in a 3-D environment and that this spatial context is necessary for development of cultures that more realistically act like in vivo tissues and organs ( 25 ). (
  • Without a clear understanding and characterization of solitary astrocytes, we might not be able to fully appreciate their functional contribution when working with other cells in vivo . (
  • In vivo, organs communicate each other by endocrine factors to efficiently proliferate cells. (
  • Fortunately, a large number of potential carriers have been identified, primarily for use in cell culture, but with increasing reports of successful in vivo use as well ( 1 ). (
  • For stem cell applications, serum-free media that lack growth factors, cytokines, or artificial stimulators of proliferation will play an increasingly important role. (
  • In addition, media and serum used in stem cell research are priced higher than similar, non-stem cell products. (
  • The round - backed by Beyond Next Ventures, multinational food company NH Foods, AgFunder and other investors - follows a US$2.7m seed round raised in 2018, and will be used to fund R&D work, build the production facility for foie gras, and support the launch of a cosmetics ingredient based on the company's cultured serum, said CEO Yuki Hanyu. (
  • A second generation prototype, consisting of a bioreactor chamber system accommodating a six-well plate format, has been recently developed, undergone preliminary characterization, and tested under a limited number of cell culture conditions. (
  • The next generation of seasonal influenza and pandemic vaccines will be produced by cell culture-based technology. (
  • Cell Lines - Our partner labs will isolate cells of any description from specific organs or tissues (any animal or human tissues) designated. (
  • Primary Cells - our partner labs are able to isolate cells of any description from specific organs or tissues (any animal or human tissues) designated. (
  • Whether they are provided either the already excised tissues or live mice or rats, the lab will isolate the desired cell type and return a vial containing the prepared cells ready to use. (
  • 20 ) reported production of Norwalk virus particles (norovirus GI.1, the prototype strain of this genera from Norwalk, Ohio) after transinfection of cultured kidney cells. (
  • In other words, if your kidney cancer cell isn't really a kidney cancer line, then how will anyone else be able to reproduce your work? (
  • The diagnosis is ultimately based on the presence of plasma cells in the endometrial stroma upon histopathological examination. (
  • Examination of intracellular Ca 2+ oscillations in solitary astrocytes cultured in micropits revealed less variable oscillations than those of non-micropit grouped astrocytes, which were in contact with their neighbors. (
  • Bone marrow culture is an examination of the soft, fatty tissue found inside certain bones. (
  • Following approval of the application by the Cell Culture Center's Scientific Advisory Board, the applicant's cell line is sent to the Center, and grown to the requested amount. (
  • As the world's population increases, cultivated or lab-grown meat-animal muscle and fat cells grown in laboratory conditions-has emerged as a potential way to satisfy future protein needs. (
  • Cell culture is the process by which cells are grown under controlled conditions. (
  • To confirm the presence of TSV in the cell culture media, a bioassay was performed by using media from the third passage. (
  • We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. (
  • 1998. Bioassay-directed chemical analysis of Los Angeles airborne particulate matter using a human cell mutagenicity assay. (
  • This image shows mouse muscle cells viewed under a microscope. (
  • The team will now exploit the disruptive and transformative power of macromolecular crowding in the applied fields of cell culture technologies, medical devices and regenerative medicine, offering new opportunities for scientific, social and economic impact. (
  • The basal medium (which circulates through the interconnected flasks/tanks transporting the growth factors to the target cells) contains a variety of ingredients including sugars and amino acids, which IntegriCulture is looking to produce from algae (in collaboration with Tokyo Women's Medical University, Japan Aerospace Exploration Agency and Japan Science and Technology Agency). (
  • The cell culture industry, which began in the late 1980s from the utilization of recombinant DNA technology and cell hybridization, is, today, a major underpinning of the biopharmaceutical market. (
  • b) Schematic showing the conceptual diffusion of O2 through oxygen-permeable micropillars to oxygenate surrounding cells suspended in the Matrigel basement membrane extract. (
  • a) Multi-well sandwich assembly design with an oxygen-permeable transwell membrane in the middle layer to hold the cell cultures. (
  • Each well contains the micropillar membrane with cells, Matrigel, and media. (
  • O2 diffuses up through the micropillar membrane into the cell cultures. (
  • These procedures can be easily adapted to other environmental conditions and any mammalian cell lines cultured in suspension, so long as a sufficient number of gene sequences are publicly available. (
  • The new incubator system is designed to provide cell growth and environmental control across a range of micro plate applications comprising of cell-based screening, immuno-capture assays, microbiology, and enzyme testing. (
  • Based primarily out of North America, under USDA regulated facilities, our partnering labs enjoy a long standing and excellent history in cell production. (
  • The Center, available to researchers throughout the United States, has been established to alleviate the current shortage of facilities and expertise required to meet the cell culture needs of the biomedical research community. (
  • Researchers at the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, led by Dr. Yvonne Mast and Dr. Imen Nouioui, have published scientific descriptions for five new antibiotic-producing bacteria. (
  • Egg whites are a good tool because they are easy and cheap to obtain and they are transparent, allowing the researchers to see the cells under a microscope. (
  • A couple of years ago, researchers took a major step toward this goal by coaxing induced pluripotent stem cells (iPSCs), which are made from mature human cells, to differentiate into cells that closely resembled beta cells. (
  • At this workshop, leading experts from academia and industry will discuss factors that impact reproducibility in cell culture studies. (
  • NIH Workshop on Reproducibility in Cell Culture Studies : Day 1-Day 2, Building 35 Conference Center / NIGMS, NIH. (
  • Solitary cells in micropit regions can undergo ATP-mediated astrocyte-microglia signaling, demonstrating that this culturing method can also be used to investigate glial-glial interactions in a spatially well-defined microenvironment. (
  • Some of the complexity observed in such studies can be attributed to astrocytic interactions with other cell types such as neurons, microglia and vasculature, and also to the intercellular communication among astrocytes via gap-junctional coupling and/or extracellularly released neurotransmitters. (
  • Traditionally, the interactions of drugs and toxicants with human tissue have been investigated in a reductionist way-for example, by focusing on specific molecular targets and using single-cell-type cultures before testing compounds in whole organisms. (
  • Quantities vary depending on individual cell lines. (
  • Progress in the future will come from processes, such as metabolic engineering, that will aid in improving cell lines, which will continue to be a main focal point of research. (
  • Along these same lines, stem cell research will also add to the robust growth of the cell culture market. (
  • The ability to develop continuous cell lines has enabled mass production of desired organ cells for research and treatment benefits. (
  • Animal cell culture became a common laboratory technique in the mid-1900s, but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century. (
  • Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (
  • Traditionally, research on the replication of shrimp viruses has been based on the use of cultured fish cellular lines ( 6 ). (
  • Control cell lines were injected with filtered hepatopancreas extracts from healthy shrimp. (
  • Fresh cell lines were inoculated with the lysate, incubated, and processed in a similar way. (
  • In order to derive cell lines producing sufficient quantities of PrP(Sc) for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrP(Sc). (
  • Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. (
  • Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation. (
  • Custom Reporter Cell Lines (e.g. (
  • GFP, RFP, Luciferase, among others) - transcription factor-dependent reporter cell lines can be ideal tools for high-throughput RNAi, peptide, and chemical library screens. (
  • With lentiviral-based transduction, our partner labs can easily engineer stable reporter lines for almost any application-regardless of cell type. (
  • 70% transcript knockdown in standard cell lines as measured by qRT-PCR. (
  • A rho-associated protein kinase inhibitor (ROCK) was injected along with the cultured cells to promote cell survival and adhesion. (
  • Kalorama estimates that stem cell research accounted for nearly $1 billion million in expenditures last year, 10% of which was allocated to consumables, and about 5% of the consumable allocation was spent on media and other cell culture supplies. (
  • NEST has launched over 200 plastic consumable products for cell culture, molecular bislogy, immunoassays, liquid handling and storage. (
  • Biomedical research at the basic levels will become a major driver of innovation for the cell culture market. (
  • Biomedical research and bioprocessing have a wide range of supply needs, including high-quality media and reagents for fermentation and cell culture. (
  • Watch our recent webinar to learn more about new methods for 3D cell culturing and how to apply them to high throughput screening assays. (
  • Responsive reporter cells form the core of cell-based assays for screening and identifying drugs, antibodies, cell factors, and other effectors. (
  • The cells have fused together to form myotubes that have many nuclei (stained blue). (
  • Blue indicates cell nuclei. (
  • The use of undefined raw materials, specifically hydrolysates, often enhances the productivity of a culture, but it may also contribute to variability. (
  • Because each mutant has only a few progeny, the overall biological properties of the cell culture are not altered unless a mutation provides a survival or growth advantage. (
  • The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (
  • Next, small cellular groups and some isolated round cells were observed. (
  • The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (
  • Tokyo-based cell-cultured meat company IntegriCulture has raised JPY 800m (US$ 7.4m) in a Series A round that will help it advance its mission of 'democratizing cellular agriculture' and build a production facility to take slaughter-free foie gras to market in 2021. (
  • It is not certain how low (less than 0.1 Gy) doses of IR affect human cells, and which signaling networks and pathways underlie cellular responses to such IR exposures. (
  • Cytopathic effect and norovirus RNA were detected at each of the 5 cell passages for genogroup I and II viruses. (
  • No growth of bacteria, viruses, or fungi in the culture is normal. (
  • We showed that astrocytes cultured in micropit regions were viable and exhibited similar characteristics of Ca 2+ dynamics and astrocytic marker expression to those of cells cultured in non-micropit regions. (
  • The bioreactor setup allows for multiple 6-well plates increasing experimental throughput, and in particular: to enable multiple parallel culture conditions under controlled hypoxia and to expand the potential application of the system to a variety of problems, including drug-screening studies. (
  • Scaling is on the way, but it is somewhat tricky to say '8kg cell mass per month per bioreactor' is scaled. (
  • Media, sera, and reagents are the fuel that powers these cell culture manufacturing engines, and suppliers are beginning to see sales ramping up accordingly. (
  • According to Kalorama's Cell Culture: The World Market for Media, Sera, and Reagents, the market for these products is growing at an annual rate in excess of 12% and headed for more than $2.6 billion in 2011. (
  • Good manufacturing practice (GMP) guidelines are particularly important for the media and reagents used in the development and manufacture of cell therapies. (
  • 1997. Human cell mutagens in Los Angeles air. (
  • 1996. Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols. (
  • RxPG] More and more laboratories are seeking to develop three-dimensional cell culture systems that allow them to test their new techniques and drugs in a system that more closely mimics the way in which cells grow. (
  • His ERC Proof of Concept project, DECIPHER - Decellularised Extracellular Carpets for the Innovative Production of Human Engineered Replicates , will build on a ERC Consolidator Award he received in 2019 to investigate the fundamentals of macromolecular crowding in cell culture systems. (
  • Preliminary data illustrate that the use of macromolecular crowding in cell culture not only offers control over cell fate, but also allows for the accelerated development of functional and truly three-dimensional tissue-like moduli, using only a fraction of cells and time that traditional approaches require. (
  • The growing use and diverse applications of stem cells are having a significant impact on the media market, as companies work to understand how best to optimize and influence their growth. (
  • The cells are from mouse skeletal muscle stem cells treated with a harmless virus that caused them to glow green. (
  • The green color remained when the stem cells fused into myotubes. (
  • Human pluripotent stem cells are cells that have two very important traits-a seemingly endless proliferative capability and the amazing ability to give rise to any cell in our body. (
  • Genomic alterations in cultured human embryonic stem cells. (
  • Insulin-containing pancreatic beta cells (green) derived from human stem cells. (
  • Recently emerging Chimeric Antigen Receptor (CAR) T cell therapy has shown great promise for cancer treatment. (
  • Nevertheless, these dividing cells, like other cells, probably undergo spontaneous mutation at a rate of 10(-9) per nucleotide. (
  • In our In Focus feature on RegMedNet, we'll look at the benefits of using GMP grade cell culture media, such as ensuring product consistency and potency, and how it can simplify regulatory approval and accelerate time to market for cell therapy products. (
  • RegMedNet interviewed our Material Compliance Manager, Maximilian Gert-Kleint, about what it means for cell culture media to be GMP grade and the importance of this in the context of cell therapy manufacturing. (
  • Our expert also shares his views on the future of cell culture media for cell therapy applications. (
  • From buffer solutions to cell culture media formulations at bench top or production sales, Caisson Labs gives you the products you need in a way that makes sense to you. (
  • And we continue to support you with a diverse and evolving portfolio of innovative 3D cell culture products, like the Corning spheroid microplate , as well as workflow solutions, protocols, and expertise. (
  • Growth in biopharmaceuticals is creating an unprecedented increase in demand for cell culture products. (
  • Furthermore, increasing incidence of breast cancer is expected to drive growth of the market during the forecast period, owing to robust pipeline of cell culture products. (
  • Utilizing GMP grade products for cell therapy manufacturing: Learn more about the impact GMP grade products have on patient safety, product consistency and efficacy, traceability and documentation, and obtaining the necessary regulatory approvals for the clinical application of cell therapies. (
  • In the biotechnology industry, process optimization and performance robustness studies are key to achieving efficient, reliable, and high yield cell culture processes. (
  • and, we examine the magnitude of transcriptional changes in some well-known radiation-responsive set of genes, such as CDKN1A, GADD45A, GDF15, etc. in more focused studies in these cells. (
  • The feeder cells can stay effective for a very long time: at least 250 days have been demonstrated ​[but they could] probably ​[last] more than two years, ​" he added. (
  • At about this time, in fact it was rather early on, a fellow who I had known for many years, a cell culturist, who. (
  • who worked at the National Institutes of Health, a man by the name of Robert Stevenson, who was also a cell culture pioneer, but by this time he had an administrative job at the NIH. (