Aquaporin 1 forms a water-specific channel that is constitutively expressed at the PLASMA MEMBRANE of ERYTHROCYTES and KIDNEY TUBULES, PROXIMAL. It provides these cells with a high permeability to WATER. In humans polymorphisms of this protein result in the Colton blood group antigen.
Aquaporin 5 is a water-specific channel protein that is expressed primarily in alveolar, tracheal, and upper bronchial EPITHELIUM. It plays an important role in maintaining water HOMEOSTASIS in the LUNGS and may also regulate release of SALIVA and TEARS in the SALIVARY GLANDS and the LACRIMAL GLAND.
Aquaporin 3 is an aquaglyceroporin that is expressed in the KIDNEY COLLECTING DUCTS and is constitutively localized at the basolateral MEMBRANE.
Aquaporin 4 is the major water-selective channel in the CENTRAL NERVOUS SYSTEM of mammals.
A class of porins that allow the passage of WATER and other small molecules across CELL MEMBRANES.
Aquaporin 2 is a water-specific channel protein that is expressed in KIDNEY COLLECTING DUCTS. The translocation of aquaporin 2 to the apical PLASMA MEMBRANE is regulated by VASOPRESSIN, and MUTATIONS in AQP2 have been implicated in a variety of kidney disorders including DIABETES INSIPIDUS.
Aquaporin 6 is an aquaglyceroporin that is found primarily in KIDNEY COLLECTING DUCTS. AQP6 protein functions as an anion-selective channel.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Mercury chloride (HgCl2). A highly toxic compound that volatizes slightly at ordinary temperature and appreciably at 100 degrees C. It is corrosive to mucous membranes and used as a topical antiseptic and disinfectant.
Tendency of fluids (e.g., water) to move from the less concentrated to the more concentrated side of a semipermeable membrane.
A subgroup of aquaporins that transport WATER; GLYCEROL; and other small solutes across CELL MEMBRANES.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
The balance of fluid in the BODY FLUID COMPARTMENTS; total BODY WATER; BLOOD VOLUME; EXTRACELLULAR SPACE; INTRACELLULAR SPACE, maintained by processes in the body that regulate the intake and excretion of WATER and ELECTROLYTES, particularly SODIUM and POTASSIUM.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The loss of water vapor by plants to the atmosphere. It occurs mainly from the leaves through pores (stomata) whose primary function is gas exchange. The water is replaced by a continuous column of water moving upwards from the roots within the xylem vessels. (Concise Dictionary of Biology, 1990)
Straight tubes commencing in the radiate part of the kidney cortex where they receive the curved ends of the distal convoluted tubules. In the medulla the collecting tubules of each pyramid converge to join a central tube (duct of Bellini) which opens on the summit of the papilla.
The ability of the kidney to excrete in the urine high concentrations of solutes from the blood plasma.
A genetic or acquired polyuric disorder characterized by persistent hypotonic urine and HYPOKALEMIA. This condition is due to renal tubular insensitivity to VASOPRESSIN and failure to reduce urine volume. It may be the result of mutations of genes encoding VASOPRESSIN RECEPTORS or AQUAPORIN-2; KIDNEY DISEASES; adverse drug effects; or complications from PREGNANCY.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.
A syndrome characterized by acute OPTIC NEURITIS; MYELITIS, TRANSVERSE; demyelinating and/or necrotizing lesions in the OPTIC NERVES and SPINAL CORD; and presence of specific autoantibodies to AQUAPORIN 4.
Urination of a large volume of urine with an increase in urinary frequency, commonly seen in diabetes (DIABETES MELLITUS; DIABETES INSIPIDUS).
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
Agents that reduce the excretion of URINE, most notably the octapeptide VASOPRESSINS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A plant genus of the family LILIACEAE. Members contain tuliposides and tulipalins and have been associated with allergic contact dermatitis in florists.
The contribution to barometric PRESSURE of gaseous substance in equilibrium with its solid or liquid phase.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. The common name of rock rose is used with several plants of this family.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
Increased intracellular or extracellular fluid in brain tissue. Cytotoxic brain edema (swelling due to increased intracellular fluid) is indicative of a disturbance in cell metabolism, and is commonly associated with hypoxic or ischemic injuries (see HYPOXIA, BRAIN). An increase in extracellular fluid may be caused by increased brain capillary permeability (vasogenic edema), an osmotic gradient, local blockages in interstitial fluid pathways, or by obstruction of CSF flow (e.g., obstructive HYDROCEPHALUS). (From Childs Nerv Syst 1992 Sep; 8(6):301-6)
Inorganic compounds that contain mercury as an integral part of the molecule.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Fluids composed mainly of water found within the body.
Drugs used for their effects on the kidneys' regulation of body fluid composition and volume. The most commonly used are the diuretics. Also included are drugs used for their antidiuretic and uricosuric actions, for their effects on the kidneys' clearance of other drugs, and for diagnosis of renal function.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
Antidiuretic hormones released by the NEUROHYPOPHYSIS of all vertebrates (structure varies with species) to regulate water balance and OSMOLARITY. In general, vasopressin is a nonapeptide consisting of a six-amino-acid ring with a cysteine 1 to cysteine 6 disulfide bridge or an octapeptide containing a CYSTINE. All mammals have arginine vasopressin except the pig with a lysine at position 8. Vasopressin, a vasoconstrictor, acts on the KIDNEY COLLECTING DUCTS to increase water reabsorption, increase blood volume and blood pressure.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The condition that results from excessive loss of water from a living organism.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A phylum of fungi that are mutualistic symbionts and form ARBUSCULAR MYCORRHIZAE with PLANT ROOTS.
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
The body of a fungus which is made up of HYPHAE.
A synthetic analog of the pituitary hormone, ARGININE VASOPRESSIN. Its action is mediated by the VASOPRESSIN receptor V2. It has prolonged antidiuretic activity, but little pressor effects. It also modulates levels of circulating FACTOR VIII and VON WILLEBRAND FACTOR.
A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.
Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.
A plant genus of the family AIZOACEAE. It is a native of Africa and widely planted for erosion control to stabilize soil along roadsides and beaches.
The internal portion of the kidney, consisting of striated conical masses, the renal pyramids, whose bases are adjacent to the cortex and whose apices form prominent papillae projecting into the lumen of the minor calyces.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed)
Specific molecular sites or proteins on or in cells to which VASOPRESSINS bind or interact in order to modify the function of the cells. Two types of vasopressin receptor exist, the V1 receptor in the vascular smooth muscle and the V2 receptor in the kidneys. The V1 receptor can be subdivided into V1a and V1b (formerly V3) receptors.
Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.
A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)
An enzyme that catalyzes the formation of glycerol 3-phosphate from ATP and glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. EC
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A plant genus of the family ROSACEAE known for the edible fruit.
A mutant strain of Rattus norvegicus used in research on renal function and hypertension and as a disease model for diabetes insipidus.
Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere.
Protein-lipid combinations abundant in brain tissue, but also present in a wide variety of animal and plant tissues. In contrast to lipoproteins, they are insoluble in water, but soluble in a chloroform-methanol mixture. The protein moiety has a high content of hydrophobic amino acids. The associated lipids consist of a mixture of GLYCEROPHOSPHATES; CEREBROSIDES; and SULFOGLYCOSPHINGOLIPIDS; while lipoproteins contain PHOSPHOLIPIDS; CHOLESTEROL; and TRIGLYCERIDES.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Hypertonic sodium chloride solution. A solution having an osmotic pressure greater than that of physiologic salt solution (0.9 g NaCl in 100 ml purified water).
A schistosomicide possibly useful against other parasites. It has irritant emetic properties and may cause lethal cardiac toxicity among other adverse effects.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Slender tubular or hairlike excretory structures found in insects. They emerge from the alimentary canal between the mesenteron (midgut) and the proctodeum (hindgut).
An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Liquids transforming into solids by the removal of heat.
Na-K-Cl transporter in the ASCENDING LIMB OF LOOP OF HENLE. It mediates active reabsorption of sodium chloride and is inhibited by LOOP DIURETICS such as FUROSEMIDE; and BUMETANIDE. Mutations in the gene encoding SLC12A1 are associated with a BARTTER SYNDROME.
A cytotoxic sulfhydryl reagent that inhibits several subcellular metabolic systems and is used as a tool in cellular physiology.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The quantity of volume or surface area of CELLS.
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A plant genus of the family FAGACEAE that is a source of TANNINS. Do not confuse with Holly (ILEX).
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.
A computer simulation developed to study the motion of molecules over a period of time.
Adaptation to a new environment or to a change in the old.
The relationships of groups of organisms as reflected by their genetic makeup.
Starches that have been chemically modified so that a percentage of OH groups are substituted with 2-hydroxyethyl ether groups.
A ubiquitous sodium salt that is commonly used to season food.
2001). "Expression and localization of epithelial aquaporins in the adult human lung". Am. J. Respir. Cell Mol. Biol. 24 (3): ... Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 ... Aquaporin-5 is a protein that in humans is encoded by the AQP5 gene. Aquaporin 5 (AQP5) is a water channel protein. ... Cell Res. 313 (12): 2695-702. doi:10.1016/j.yexcr.2007.02.035. PMID 17553491. AQP5+protein,+human at the US National Library of ...
Cell and Tissue Research, 223(2), 281-286. Lindsay, L. A., & Murphy, C. R. (2004). Redistribution of aquaporins in uterine ... Uterine epithelial cells have a similar structure in most species and the changes which occur in the uterine epithelial cells ... Like all epithelial cells, the uterine epithelial cells lie on a basal lamina. The apical plasma membrane displays ... The cytoplasm of uterine epithelial cells contains typical organelles found in other cells, including a nucleus, which is ...
Aquaporins are "the plumbing system for cells," said Agre. Every cell is primarily water. "But the water doesn't just sit in ... Aquaporins are water-channel proteins that move water molecules through the cell membrane. In 2009, Agre was elected president ... "for discoveries concerning channels in cell membranes." Credited for discovery of the aquaporin water channels, Agre was in bed ... Agre joined Vann Bennett's lab in the Cell Biology Department at Johns Hopkins where he studied red cell membranes and ...
... the distal lung epithelial development is stunted in transgenic mice models The progenitor cells, or stem cells, for alveolar ... also indicated in proper lung development by controlling the late differentiation stages of alveolar epithelium and aquaporin-5 ... Cell. Biol. 23 (18): 6646-61. doi:10.1128/MCB.23.18.6646-6661.2003. PMC 193708. PMID 12944489. Jimenez P, Saner K, Mayhew B, ... Cell. 4 (1): 107-18. doi:10.1016/S1534-5807(02)00396-9. PMID 12530967. Nemer G, Nemer M (2003). "Transcriptional activation of ...
Aquaporins are "the plumbing system for cells". Water moves through cells in an organized way, most rapidly in tissues that ... The cell membranes of a variety of different bacteria, fungi, animal and plant cells contain aquaporins through which water can ... Because aquaporins are usually always open and are prevalent in just about every cell type, this leads to a misconception that ... Aquaporin proteins are composed of a bundle of six transmembrane α-helices. They are embedded in the cell membrane. The amino ...
2005). "Aquaporin-3 expression in human fetal airway epithelial progenitor cells". Stem Cells. 23 (7): 992-1001. doi:10.1634/ ... increases expression of aquaporin-3 in normal skin cells (keratinocytes). Aquaporin 3 levels are often lower in psoriasis than ... Aquaporin 3 is the protein product of the human AQP3 gene. It is found in the basolateral cell membrane of principal collecting ... 2005). "Roles of aquaporin-3 water channels in volume-regulatory water flow in a human epithelial cell line". J. Membr. Biol. ...
... so the aquaporin 2 can be used by the cell. This aquaporin is regulated in two ways by the peptide hormone vasopressin: short- ... Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, ... Robben JH, Knoers NV, Deen PM (August 2006). "Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water ... It is the only aquaporin regulated by vasopressin. The basic job of aquaporin 2 is to reabsorb water from the urine while its ...
... in the cell plasma membrane. Aquaporin-4 is the most common aquaporin in the brain, spinal cord, and optic nerve. It is highly ... "Aquaporin-4 in Astroglial Cells in the CNS and Supporting Cells of Sensory Organs-A Comparative Perspective". International ... April 1998). "Aquaporin-4 water channel protein in the rat retina and optic nerve: polarized expression in Müller cells and ... The expression of aquaporin 4 is reliant on the disease stage of TBI. In an acute stage of TBI, the lack of aquaporin 4 causes ...
The secretory activities of the gland cells and the contractions of myoepithelial cells are controlled by both the autonomic ... can sometimes come with abnormal aquaporin 5 in the sweat glands. Cystic fibrosis can be diagnosed by a sweat test, as the ... Secretion of sweat occurs when the myoepithelial cell cells surrounding the secretory glands contract. Eccrine sweat increases ... on the cell surface, however, recent electron micrographs indicate that the cells use merocrine secretion. In both apocrine and ...
... elegans by downregulating DAF-16/FOXO activity and aquaporin gene expression". Cell Metabolism. 10 (5): 379-91. doi:10.1016/j. ... doi:10.1016/j.cell.2005.02.002. PMID 15734678. Hu, 2007 Hu, P.J. (2007). Dauer. In WormBook, The C. elegans Research Community ... Apfeld J, Kenyon C (October 1998). "Cell nonautonomy of C. elegans daf-2 function in the regulation of diapause and life span ... DAF-16 is responsible for up-regulating transcription of about 100 genes that code for cell protecting products such as heat ...
"Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells". Journal of Cell ... colocalization with aquaporin-2 in collecting duct vesicles". The American Journal of Physiology. 275 (5 Pt 2): F752-60. doi: ... "Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells ... Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is ...
"Syntaxin-4 is localized to the apical plasma membrane of rat renal collecting duct cells: possible role in aquaporin-2 ... Cell. 74 (5): 863-73. doi:10.1016/0092-8674(93)90466-4. PMID 7690687. S2CID 37698687. Li C, Ullrich B, Zhang JZ, Anderson RG, ... Journal of Cell Science. 115 (Pt 6): 1321-30. PMID 11884531. Polgár J, Chung SH, Reed GL (Aug 2002). "Vesicle-associated ... colocalization with aquaporin-2 in collecting duct vesicles". The American Journal of Physiology. 275 (5 Pt 2): F752-60. doi: ...
1999 Aquaporin-1 is expressed by vascular smooth muscle cells and mediates rapid water transport across vascular cell membranes ... "Aquaporin-1 is expressed by vascular smooth muscle cells and mediates rapid water transport across vascular cell membranes". J ... Cancer Cell, 2019 Engineering of a GLP-1 analogue peptide/anti-PCSK9 antibody fusion for type 2 diabetes treatment, Sci Rep, ... Cancer Cell. 35 (6): 948-949. doi:10.1016/j.ccell.2019.05.010. PMID 31185215. Chodorge, M (3 December 2018). "Engineering of a ...
Otto, B. and R. Kaldenhoff, Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana ... Aquaporin tetramer composition modifies the function of tobacco aquaporins. Journal of Biological Chemistry, 2010. 285(41): p. ... Plant Cell, 2008. 20(3): p. 648-57. Flexas, J., M. Ribas-Carbo, D.T. Hanson, J. Bota, B. Otto, J. Cifre, N. McDowell, H. ... He is known for his work on the aquaporin protein class, where he detected facilitated diffusion of CO2 in plant tissue and ...
2003). "Aquaporin-9 is expressed in a mucus-secreting goblet cell subset in the small intestine". FEBS Lett. 540 (1-3): 157-62 ... Aquaporin-9 is a protein that in humans is encoded by the AQP9 gene. The aquaporins/major intrinsic protein are a family of ... Loitto VM, Huang C, Sigal YJ, Jacobson K (2007). "Filopodia are induced by aquaporin-9 expression". Exp. Cell Res. 313 (7): ... Aquaporin 9 has greater sequence similarity with AQP3 and AQP7 and they may be a subfamily. Aquaporin 9 allows passage of a ...
... into the apical membrane of collecting tubule and collecting duct epithelial cells. Aquaporins allow water to move down their ... Day TA, Sibbald JR (June 1990). "Noxious somatic stimuli excite neurosecretory vasopressin cells via A1 cell group". The ... thus increasing the total number of aquaporin-2 molecules in collecting duct cells. Increasing permeability of the inner ... Some forms of cancer can cause SIADH, particularly small cell lung carcinoma but also a number of other tumors. A variety of ...
In cells of these organs, NKCC1 is commonly found in the basolateral membrane, the part of the cell membrane closest to the ... Increased NKCC2 activity aids in water reabsorption in the collecting duct through aquaporin 2 channels by creating a hypo- ... Within these cells, NKCC2 resides in the apical membrane abutting the nephron's lumen, which is the hollow space containing ... However, there is a notable exception in squid giant axon as the symporter in this special cell has a stoichiometry of 2Na:1K: ...
Robben JH, Knoers NV, Deen PM (Aug 2006). "Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water ... AVPR2 is expressed in the kidney tubule, predominantly in the membrane of cells of the distal convoluted tubule and collecting ... Stimulation causes the release of von Willebrand factor and factor VIII from the endothelial cells. Because von Willebrand ... Ishikawa SE (Feb 2002). "[Nephrogenic diabetes insipidus associated with mutations of vasopressin V2 receptors and aquaporin-2 ...
Smith BL, Preston GM, Spring FA, Anstee DJ, Agre P (September 1994). "Human red cell aquaporin CHIP. I. Molecular ... Aquaporin+1 at the US National Library of Medicine Medical Subject Headings (MeSH) Gallery of Aquaporin Simulations Human AQP1 ... "Entrez Gene: AQP1 aquaporin 1 (Colton blood group)". Knepper MA (July 1994). "The aquaporin family of molecular water channels ... The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Aquaporin ...
He is professor and chairman in the Department of Cell and Molecular Biology of the Iuliu Hațieganu University of Medicine and ... Kuchel PW (2006). "The story of the discovery of aquaporins: convergent evolution of ideas--but who got there first?". Cellular ... Identification of membrane proteins involved in water transport". European Journal of Cell Biology. 41 (2): 252-262. PMID ... naming it aquaporin. In 2003 Agre would receive the Nobel Prize in Chemistry for his work. Benga and his collaborators would ...
Fischer H, Stenling R, Rubio C, Lindblom A (2003). "Differential expression of Aquaporin 8 in human colonic epithelial cells ... Aquaporin-8 is a protein that in humans is encoded by the AQP8 gene. Aquaporin 8 (AQP8) is a water channel protein. Aquaporins ... Cytogenet Cell Genet. 84 (3-4): 208-10. doi:10.1159/000015260. PMID 10393433. S2CID 23854978. "Entrez Gene: AQP8 aquaporin 8". ... "Expression of aquaporin 8 and its up-regulation by cyclic adenosine monophosphate in human WISH cells". Am. J. Obstet. Gynecol ...
Two types of aquaporins are expressed in the CNS: aquaporin-1, which is expressed by specialized epithelial cells of the ... Aquaporins are membrane-bound channels that play critical roles in regulating the flux of water into and out of cells. Relative ... The meningeal lymphatics also carry immune cells. The extent to which these cells may interact directly with the brain or ... She called it the glymphatic system, a nod to its dependence on glial cells Louveau A, Smirnov I, Keyes TJ, Eccles JD, Rouhani ...
Aquaporin-1 (Aqp1) from the human red blood cell has been solved by electron crystallography to 3.8 Å resolution (PDB: 1FQY​). ... The MIP superfamily includes three subfamilies: aquaporins, aquaglyceroporins and S-aquaporins. The aquaporins (AQPs) are water ... Chrispeels MJ, Agre P (1994). "Aquaporins: water channel proteins of plant and animal cells". Trends Biochem. Sci. 19 (10): 421 ... of the protein in the lens cell. In the native form, it is an aquaporin (AQP), but during lens development, it becomes ...
The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor ... and aquaporin-1 in human erythrocyte membrane domains". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1828 (3): 956-66. ... Cell. 132 (6): 1039-48. doi:10.1016/j.cell.2008.01.042. PMID 18358815. S2CID 18128118. Lay summary - ScienceDaily (March 21, ... "A novel inhibitor of glucose uptake sensitizes cells to FAS-induced cell death". Molecular Cancer Therapeutics. 7 (11): 3546-55 ...
Cell Biology. 37 (11): 2273-83. doi:10.1016/j.biocel.2005.02.028. PMID 16002321. Cardoso C, Leventer RJ, Ward HL, Toyo-Oka K, ... 24 (4): 1001-5. doi:10.1042/bst0241001. PMID 8968500. Mitchell CA, Brown S, Campbell JK, Munday AD, Speed CJ (Nov 1996). " ... The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal ... This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in ...
ADH binds to principal cells in the collecting duct that translocate aquaporins to the membrane, allowing water to leave the ... calbindin expressed in distal tubules and aquaporin 2 expressed in the collecting duct cells.[14] ... Interstitial kidney cells. Gene and protein expression[edit]. Further information: Bioinformatics § Gene and protein expression ... It stimulates erythropoiesis (production of red blood cells) in the bone marrow. Calcitriol, the activated form of vitamin D, ...
Streptavidin binds to four biotins per molecule.) This tetramer reagent will specifically label T cells that express T cell ... some aquaporins, some AMPA receptors, as well as some enzymes. Ion-exchange chromatography is useful for isolating specific ... Antigen specific responses can be measured as CD8+, tetramer+ T cells as a fraction of all CD8+ lymphocytes. The reason for ... In immunology, MHC tetramers can be used in tetramer assays, to quantify numbers of antigen-specific T cells (especially CD8+ T ...
The protein encoded by this gene is an aquaporin protein, which functions as a water channel in cells. Aquaporins are a family ... "Characterization of aquaporin-6 as a nitrate channel in mammalian cells. Requirement of pore-lining residue threonine 63". The ... "Entrez Gene: Aquaporin 6, kidney specific". Retrieved 2012-04-10. Human AQP6 genome location and AQP6 gene details page in the ... Wang W, Hart PS, Piesco NP, Lu X, Gorry MC, Hart TC (Mar 2003). "Aquaporin expression in developing human teeth and selected ...
The plant then reduces the impact of high Na+ abundance by improving Na+ efflux from its cells through SOS pathway Two ... Molecular and Cellular Features of Aquaporin Expression". Plant Physiology. 139 (2): 790-805. doi:10.1104/pp.105.065029. PMC ... It has been found in many studies that Arabidopsis thaliana showed enhanced Na+ and H+ extrusion from their cells after ... Babourina, O. (2000). "Effect of Sudden Salt Stress on Ion Fluxes in Intact Wheat Suspension Cells". Annals of Botany. 85 (6): ...
"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites". ... In SILAC, cells are cultured separately in the presence of different isotopically labeled amino acids for several cell ... In one study design, cells are exposed to SILAC labelling and then stimulated by a specific growth factor. The cells are ... A sample large-scale phosphoproteomic analysis includes cultured cells undergo SILAC encoding; cells are stimulated with factor ...
cell junction. • dendrite. • sarcolemma. • cell projection. • perikaryon. • synapse. • postsynaptic membrane. Biological ... membrane depolarization during atrial cardiac muscle cell action potential. • cardiac muscle cell action potential involved in ... "Cell and Tissue Research. 357 (2): 463-76. doi:10.1007/s00441-014-1936-3. PMID 24996399.. ... cell communication by electrical coupling involved in cardiac conduction. • regulation of ion transmembrane transport. • ion ...
Aldosterone normally adds sodium channels in the principal cells of the collecting duct and late distal tubule of the nephron. ... Competitive vasopressin antagonism leads to decreased number of aquaporin channels in the apical membrane of the renal ... Osmotic diuretics (e.g. mannitol) are substances that increase osmolarity but have limited tubular epithelial cell permeability ... Spironolactone prevents aldosterone from entering the principal cells, preventing sodium reabsorption. Similar agents are ...
Aquaporin 1 - water transporter, defines the Colton Blood Group;. *Glut1 - glucose and L-dehydroascorbic acid transporter; ... Red blood cells, also known as RBCs, red cells,[1] red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ... Red blood cells in mammals anucleate when mature, meaning that they lack a cell nucleus. In comparison, the red blood cells of ... Red blood cells are cells present in blood in order to transport oxygen. The only known vertebrates without red blood cells are ...
... it acts on proteins called aquaporins and more specifically aquaporin 2 in the following cascade. When released, ADH binds to ... These transcribed channels allow water into the collecting duct cells. The increase in permeability allows for reabsorption of ... Nephrogenic DI results from lack of aquaporin channels in the distal collecting duct (decreased surface expression and ... stimulating translocation of the aquaporin 2 channel stored in the cytoplasm of the distal convoluted tubules and collecting ...
"Seminars in Cell & Developmental Biology. 17 (6): 667-74. doi:10.1016/j.semcdb.2006.11.003. PMC 2705932. PMID 17116414.. ... Heiner I, Eisfeld J, Lückhoff A (2004). "Role and regulation of TRP channels in neutrophil granulocytes". Cell Calcium. 33 (5-6 ... inositol 1,4,5 trisphosphate binding. • ion channel activity. • protein binding. • actin binding. • calcium channel activity. • ... It has been associated with depression and anxiety (see below), as well as with focal segmental glomerulosclerosis (FSGS).[5] ...
... and is necessary for the control of cellular and physiological processes including cell division, cell proliferation, apoptosis ... inositol 1,4,5-trisphosphate receptor, type 1[1]. Crystal structure of the ligand binding suppressor domain of type 1 inositol ... The initial sequencing was reported as an unknown protein enriched in the cerebellum called P400.[5] The large size of this ... December 2002). "Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand". Nature. 420 ( ...
The unit cell of this compound is about 15 by 25 Ångström. It is outlined at the centre of the figure, inside the result from ... highest resolution protein structure solved by electron crystallography of 2D crystals is that of the water channel aquaporin-0 ... These d-values range from the unit cell dimensions to the resolution limit of the electron microscope, i.e. typically from 10 ... 26 (5): 759-766.e4. doi:10.1016/j.str.2018.03.021. ISSN 0969-2126. PMC 6333475. PMID 29706530.. ...
2004). „Antidiuretic action of oxytocin is associated with increased urinary excretion of aquaporin-2". Nephrology, Dialysis, ... anat/phys/devp/horm/cell noco(d)/cong/tumr, sysi/epon proc, lek (A10/H1/H2/H3/H5) ...
Such molecules can diffuse passively through protein channels such as aquaporins in facilitated diffusion or are pumped across ... Hooke misled the cell membrane theory that all cells contained a hard cell wall since only plant cells could be observed at the ... The cell membrane, being exposed to the outside environment, is an important site of cell-cell communication. As such, a large ... Main article: History of cell membrane theory. While Robert Hooke's discovery of cells in 1665 led to the proposal of the Cell ...
"Cell. 140 (1): 88-98. doi:10.1016/j.cell.2009.12.024. PMC 2885139. PMID 20074522.. ... Figure 1. Whole-cell current recordings of Kir2 inwardly-rectifying potassium channels expressed in an HEK293 cell. (This is a ... Kir channels are found in multiple cell types, including macrophages, cardiac and kidney cells, leukocytes, neurons, and ... into the cell) than in the outward direction (out of the cell). It is thought that this current may play an important role in ...
aquaporin 5, calmodulin, pacsin 3 2 TRPV5 calcium-selective TRP channel intestine, kidney, placenta 100:1 TRPV6 annexin II / ... Later, TRP channels were found in vertebrates where they are ubiquitously expressed in many cell types and tissues. There are ... located mostly on the plasma membrane of numerous human and animal cell types, and in some fungi.[2] TRP channels were ... about 28 TRP channels that share some structural similarity to each other.[5] These are grouped into two broad groups: group 1 ...
principal cells in kidney. kidney. *Vasopressin → V2 receptor. *theophylline (PDE inhibitor). *exocytosis of aquaporin 2 to ... Thus, the effects of PKA activation vary with cell type: Overview tableEdit. Cell type Organ/system Stimulators ligands → Gs- ... As protein expression varies from cell type to cell type, the proteins that are available for phosphorylation will depend upon ... In cell biology, protein kinase A (PKA[N 1]) is a family of enzymes whose activity is dependent on cellular levels of cyclic ...
cell-cell signaling. • cell communication. • protein oligomerization. • transmembrane transport. • nervous system development. ... In normal circumstances this protein is located in the cell membrane of Schwann cells and oligodendrocytes, specialised cells ... 98 (2): 172-5. doi:10.1007/s004390050183. PMID 8698335.. *^ a b c d e f g h i j "GJB1 gene". Genetics Home Reference. US ... Sato H, Hagiwara H, Ohde Y, Senba H, Virgona N, Yano T (March 2007). "Regulation of renal cell carcinoma cell proliferation, ...
positive regulation of cell migration. • secretion by cell. • secretory granule organization. • wound healing. • hyperosmotic ... Аквапорин-1, AQP1 (англ. Aquaporin 1 (Colton blood group)) - білок, який кодується геном AQP1, розташованим у людей на ... apical part of cell. • basal plasma membrane. • integral component of plasma membrane. • brush border membrane. • basolateral ... de Groot B.L., Engel A., Grubmueller H. (2001). A refined structure of human aquaporin-1.. FEBS Lett. 504: 206 - 211. PubMed ...
Evidence for a secretin-induced vesicular translocation of aquaporin-1" (PDF). J. Biol. Chem. 272 (20): 12984-12988. PMID ... Lee LT, Tan-Un KC, Chow BK (2006). "Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by ... and secretin in mouse duodenal cells". Ann. N. Y. Acad. Sci. 1070: 371-375. PMID 16888194. doi:10.1196/annals.1317.047.. ... transcriptional events controlling the terminal differentiation of intestinal endocrine cells". Aliment. Pharmacol. Ther. 14 ...
... small blue cells - juxtaglomerular cells; 4) large blue cells - mesangial cells; 5) tan - podocytes lining Bowman's capsule ... Aquaporins are membrane proteins that selectively conduct water molecules while preventing the passage of ions and other ... It contains three components: the macula densa, juxtaglomerular cells, and extraglomerular mesangial cells.[citation needed] ... This image shows the types of cells present in the glomerulus part of a kidney nephron. Podocytes, Endothelial cells, and ...
However larger molecules such as proteins and blood cells are prevented from passing through the filtration membrane. The ... water permeability of the kidney's collecting duct and distal convoluted tubule by inducing translocation of aquaporin-CD water ... 1. Human urinary system: 2. Kidney, 3. Renal pelvis, 4. Ureter, 5. Urinary bladder, 6. Urethra. (Left side with frontal section ... "Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to ...
Sodium is the primary positively charged ion outside of the cell and cannot cross from the interstitial space into the cell. ... Hyponatremia occurs in one of two ways: either the osmoreceptor-aquaporin feedback loop is overwhelmed, or it is interrupted. ... thereby creating a large polar structure too large to pass through the cell membrane: "channels" or "pumps" are required. Cell ... This system operates as follows: in some of the cells of the hypothalamus, there are osmoreceptors which respond to an elevated ...
Aquaporin 1. *Arachidonate 5-lipoxygenase. *Atrophin 1. *BH3 interacting-domain death agonist ...
Nicholas J. Birch (2012-12-02). Lithium and the Cell: Pharmacology and Biochemistry. ISBN 9780080984292. .. ... Nielsen, J.; Kwon, T.H.; Christensen, B.M.; Frokiaer, J.; Nielsen, S. (May 2008). "Dysregulation of renal aquaporins and ... Lithium possesses neuroprotective properties by preventing apoptosis and increasing cell longevity.[72] ... cell loss). Lithium has been shown to increase the level of GABA in plasma and cerebral spinal fluid.[74] Lithium counteracts ...
"Cell. 149 (7): 1607-21. doi:10.1016/j.cell.2012.04.012. PMC 3641781. PMID 22579045.. ... Aquaporins. *Chloride channels. *Outer membrane auxiliary proteins (polysaccharide transporter) - α-helical transmembrane ... Harvey Lodish etc.; Molecular Cell Biology, Sixth edition, p.546 *^ Cross, Timothy, Mukesh Sharma, Myunggi Yi, Huan-Xiang Zhou ... A transmembrane protein (TP) is a type of integral membrane protein that spans the entirety of the cell membrane. Many ...
Activation of particular VGCCs allows Ca2+ to rush into the cell, which, depending on the cell type, results in activation of ... are a group of voltage-gated ion channels found in the membrane of excitable cells (e.g., muscle, glial cells, neurons, etc.) ... neurons, cells that have pacemaker activity, bone (osteocytes) α2δ Subunit[edit]. The α2δ gene forms two subunits: α2 and δ ( ... Cerebellar granule cells, other neurons T-type calcium channel ("Transient"). low voltage activated. Cav3.1 (CACNA1G). Cav3.2 ( ...
single layer, mixed clear cells & dark cells single layer columnar cells[17]. ... can sometimes come with abnormal aquaporin 5 in the sweat glands.[70] ... Secretion of sweat occurs when the myoepithelial cell cells surrounding the secretory glands contract.[20] Eccrine sweat ... two or more layers of cuboidal cells double layer of cuboidal cells [20]. ...
When cells are compromised due to disease or injury and start to die messages are transmitted to neighboring cells connected to ... Shibata, Y; Yamamoto, T (March 1977). "Gap junctions in the cardiac muscle cells of the lamprey". Cell Tissue Res. 178 (4): 477 ... Gap junctions may be seen to function at the simplest level as a direct cell to cell pathway for electrical currents, small ... Those mRNAs appear to be down-regulated or destroyed by micro interfering RNAs ( miRNAs ) that are cell-type and cell-lineage ...
cell surface. • postsynaptic density. • cell body. • postsynaptic density membrane. • L-type voltage-gated calcium channel ... Voltage-dependent calcium channel gamma-4 subunit is a protein that in humans is encoded by the CACNG4 gene.[5][6] ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039.. *. Moss FJ, Dolphin AC, Clare JJ (2004). "Human neuronal stargazin-like ...
"Cell. 137 (4): 761-72. doi:10.1016/j.cell.2009.03.039. PMC 2719954. PMID 19450521.. ... neuronal cell body. • dendrite. • membrane raft. • cation channel complex. Biological process. • manganese ion transport. • ion ... positive regulation of cell proliferation. • positive regulation of cytosolic calcium ion concentration. • neuron ... Short transient receptor potential channel 5 (TrpC5) also known as transient receptor protein 5 (TRP-5) is a protein that in ...
cell membrane. • synapse. • integral component of plasma membrane. • cell surface. • cell junction. • neuronal cell body. • ... cell communication by electrical coupling. • neuronal signal transduction. • regulation of ion transmembrane transport. • ion ... Inosines are recognised as guanosine by the cells translational machinery. There are three members of the ADAR family ADARs 1-3 ... 24 (5): 778-82. doi:10.1002/mds.22467. PMID 19205071.. *. Imbrici P, Gualandi F, D'Adamo MC, et al. (2008). "A novel KCNA1 ...
Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha- ... Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2 gene.[5] ... "The ducky2J mutation in Cacna2d2 results in reduced spontaneous Purkinje cell activity and altered gene expression". J. ... Cell. Probes. 15 (6): 391-3. doi:10.1006/mcpr.2001.0382. PMID 11851383.. ...
At the protein level, AQP5 was significantly increased in H1299 cells compared with 16HBE cells. AQP5 knockdown in H1299 cells ... Non‑small cell lung cancer (NSCLC) constitutes the majority of all lung‑cancer cases. Aquaporin 5 (AQP5) may be involved in ... Similarly, vascular endothelial growth factor was significantly increased in control cells (si‑NC) compared with cells ... small cell lung cancer cell line H1299. *Authors: *Abdalkhalig Elkhider ...
The effects of low root temperature on growth and root cell water transport were compared between wild-type Arabidopsis ( ... Arabidopsis thaliana) and plants overexpressing plasma membrane intrinsic protein 1;4 (PIP1;4) and PIP2;5. Descending root ... temperature from 25°C to 10°C quickly reduced cell hydra … ... The effects of low root temperature on growth and root cell ... The temperature sensitivity of cell water transport in roots was reflected by a reduction in shoot and root growth rates in the ...
Effects of ACh or dbcGMP on the [Ca2+]iin Isolated Parotid Acinar Cells.. [Ca2+]i was measured in isolated parotid acinar cells ... 2001) Salivary acinar cells from aquaporin 5-deficient mice have decreased membrane water permeability and altered cell volume ... 1997) nNOS and Ca2+ influx in rat pancreatic acinar and submandibular salivary gland cells. Cell Calcium 22:217-228. ... Measurement of [Ca2+]i in Parotid Acinar Cells.. Parotid acinar cells, prepared by collagenase/hyaluronidase digestion, were ...
PCR analysis revealed 77% increase in AQP1 mRNA levels in AQP5 knockdown cells as compared to mock transfected cells (n = 3 for ... 14P - The effect of aquaporin-5 knockdown on HT29 colon cancer cell proliferation and migration. ... Aquaporins (AQPs) are a family of integral membrane proteins, known for their role in fluid homeostasis. Overexpression of AQP5 ... Proliferation and circular wound healing migration assays were performed on the transfected cells. One-way ANOVA was used for ...
Matsuzaki T, Suzuki T, Takata K (2001) Hypertonicity-induced expression of aquaporin 3 in MDCK cells. Am J Physiol Cell Physiol ... Verkman AS, Matthay MA, Song Y (2000) Aquaporin water channels and lung physiology. Am J Physiol Lung Cell Mol Physiol 278(5): ... Jenq W, Mathieson IM, Ihara W, Ramirez G (1998) Aquaporin-1: an osmoinducible water channel in cultured mIMCD-3 cells. Biochem ... The fluid-filled spheroids, derived from nasal polyps, were lined by a single cell layer with the ciliated apical cell membrane ...
... in the range of other epithelia while an exceptionally high Pf was determined for an epithelial subdomain of outer sulcus cells ... Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph- ... aquaporins, AQPs). The PD of the entire CDE (PD = 8.18 × 10(-5) cm s(-1)) and its individual partitions including Reissners ... 5) cm s(-1)) were similar to other epithelia with AQP-facilitated water permeation. The Pf of the CDE (Pf = 6.15 × 10(-4) cm s ...
These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of ... Western blot analysis confirmed their expression in four of these cell lines. In situ hybridization demonstrated that during ... Aquaporins (AQPs) are important in controlling water permeability. As AQP1 is known as a serum-responsive gene, we hypothesized ... By reverse transcriptase-polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and ...
The importance of local epithelial stem cell niches in the specification of lung stem/progenitor cells has been recently ... Studies using cell differentiating and lineage tracing assays, ,i,in vitro,/i, and/or ,i,ex vivo,/i, models, and genetically ... This paper reviews recent findings in the identification and isolation of region-specific epithelial stem/progenitor cells and ... local niches along the airway tree and the potential link of epithelial stem cells for the development of lung cancer. ...
... but not ciliated cells) also expressed the serous cell proteins lactoferrin (LTF; Raphael et al. 1989) and aquaporin 5 (AQP5; ... B, simultaneous cell volume and [Ca2+]i responses in single serous acinar cells stimulated with 100 μm CCh as indicated. Cell ... Acinar cells also expressed the serous cell proteins LTF and AQP5.. Isolated acinar cells expressed mRNA encoding Muc5AC as ... Agonist-induced cell volume changes are due to changes in cell solute content. That the observed changes in cell volume reflect ...
Rabbit polyclonal Aquaporin 5 antibody. Validated in WB, ELISA and tested in Rat. Independently reviewed in 1 review(s). ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Primary - Rabbit Anti-Aquaporin 5 antibody (ab15119) WB, ELISA Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P, WB ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ...
Anti-Aquaporin 5 Rabbit pAb Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue ... Sterile Filtration Good cell culture practices start with proven products like Stericup® and Millex® filters. ... Anti-Aquaporin 5 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available ... Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ...
Non-Small-Cell Lung / Disease-Free Survival / Aquaporins / Aquaporin 5 / Carcinogenesis / Lung Clinical aspect: Prognosis ... Aquaporins , Carcinogenesis , Carcinoma, Non-Small-Cell Lung , Carcinoma, Squamous Cell , Disease-Free Survival , Humans , Lung ... Full text: Available Index: WPRIM (Western Pacific) Main subject: Humans / Water / Carcinoma, Squamous Cell / Adenocarcinoma / ... Aquaporins are water channel proteins that play a major role in the movement of water in various human tissues. Recently, it ...
Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute ... eg.(cancer,cancers)(cervical,cervix)!("Squamous cell carcinoma",SCC). will return records where either the words cancer or ... Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute ... Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated with Pulmonary Inflammation and Survival in Acute Respiratory Distress ...
Otto, B., and Kaldenhoff, R. (2000). Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from ... Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ... The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes ... and in cells of the stomatal cavities. The aquaporin was found at sites of anticipated high water fluxes from and to the ...
... mucous cells, basal cells, and neuroendocrine cells (Figure 4). Besides Claravar cells, basal cells have also been implicated ... Aqp5, aquaporin 5; CGRP, Calcitonin gene-related peptide; Pgp9.5, protein gene product 9.5; T1α, podoplanin. ... Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A. 2009;106(31):12771-12775. ... Mitochondria and cell death: outer membrane permeabilization and beyond. Nat Rev Mol Cell Biol. 2010;11(9):621-632.. View this ...
A Crossed Pack-to-Cell Equalizer Based on Quasi-Resonant LC Converter with Adaptive Fuzzy Logic Equalization Control for Series ... Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane. ... Activity of pemetrexed-based regimen as first-line chemotherapy for advanced non-small cell lung cancer with asymptomatic ... Leibetseder, B., 2015, In: Social Policy and Administration. 49, 5, p. 549-570. Research output: Contribution to journal › ...
In the mid nineties of the last century, the OAs turned out to be a water channel named aquaporin 4 (AQP4). Since this ... cardiac muscle cells [15], light cells of the kidney collecting tubules [16], tracheal epithelial cells [17], satellite cells ... A. S. Verkman, "Aquaporin water channels and endothelial cell function," Journal of Anatomy, vol. 200, no. 6, pp. 617-627, 2002 ... syntrophin PDZ domain for the sarcolemmal localization of nNOS and aquaporin-4," Journal of Cell Biology, vol. 155, no. 1, pp. ...
Aquaporins differentially regulate cell-cell adhesion in MDCK cells.. Login FH, Jensen HH, Pedersen GA, Koffman JS, Kwon TH, ... A versatile aquaporin-2 cell system for quantitative temporal expression and live cell imaging. ... Int J Biochem Cell Biol. 2016 Oct;79:271-276. doi: 10.1016/j.biocel.2016.09.005. Epub 2016 Sep 5. Review. ... Am J Physiol Cell Physiol. 2018 Jun 1;314(6):C654-C661. doi: 10.1152/ajpcell.00186.2017. Epub 2018 Jan 31. ...
Lung fluid transport in aquaporin-5 knockout mice. J. Clin. Invest. 105: 93-100. ... Human mesenchymal stem cells induce T cell anergy and downregulate T cell allo-responses via the TH2 pathway: relevance to ... Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness. Blood 105: 2214-2219 ... Total cell count was determined with the use of a counter (Beckman Coulter). A cell smear was made using cytospin (Thermo ...
Aquaporins are "the plumbing system for cells". Water moves through cells in an organized way, most rapidly in tissues that ... The cell membranes of a variety of different bacteria, fungi, animal and plant cells contain aquaporins through which water can ... Because aquaporins are usually always open and are prevalent in just about every cell type, this leads to a misconception that ... Aquaporin proteins are composed of a bundle of six transmembrane α-helices. They are embedded in the cell membrane. The amino ...
Such membranes are from brain cells, kidney cells, muscle cells, red blood cells, and heart cells. ... Aquaporins Aquaporin-1 (AQP1) was first discovered in human red blood cell membranes by Gheorghe Bengas research group in 1986 ... Aquaporins are integral membrane proteins that specialize in the regulation of cellular water flow across the cell membrane.[3] ... Aquaporin-1 Structure Aquaporin-1 is an integral membrane protein that is considered to have an "open" structure. Despite being ...
... in the cell plasma membrane. Aquaporin-4 is the most common aquaporin in the brain, spinal cord, and optic nerve. It is highly ... "Aquaporin-4 in Astroglial Cells in the CNS and Supporting Cells of Sensory Organs-A Comparative Perspective". International ... April 1998). "Aquaporin-4 water channel protein in the rat retina and optic nerve: polarized expression in Müller cells and ... The expression of aquaporin 4 is reliant on the disease stage of TBI. In an acute stage of TBI, the lack of aquaporin 4 causes ...
Aquaporins (1)Ion Channels/biosynthesis (1) Membrane Proteins (1). Salivary Glands/metabolism (1)... View MoreDate Issued1996 ( ... Adenovirus-mediated Expression of Aquaporin-5 in Epithelial Cells Baum, Bruce J.; Delporte, Christine; Ambudkar, Indu S.; He, ... The recombinant adenovirus (AdrAQP5) mediated the expression of aquaporin-5 in rat and human salivary cell lines and in dog ... A recombinant adenovirus coding for rat aquaporin-5 was constructed and plaque purified. ...
Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin 5 Crandall, Edward D. ... Aquaporins (6)Ion Channels/genetics (3)Ion Channels/biosynthesis (2)Adenoviridae/genetics (1)Aging/metabolism (1)Aquaporins/ ... Osmotic movement of water across bacterial cell membranes is postulated to be a homeostatic mechanism for maintaining cell ... Aquaporins and the respiratory system: advice for a lung investigator Agre, Peter; Nielsen, Søren; King, Landon S. (American ...
Next, we used an in vitro system using T1α and Aqp5 (aquaporin 5) as type I cell markers (Ma et al., 2000) to further address ... For fluorescent cell sorting of Sca-1+ and Sca-1− cells, freshly isolated type II cells were resuspended to ∼1.0 × 106 cell/ml ... and the existence of two cell populations within an epithelial stem cell niche. Cell. 118:635-648. doi:10.1016/j.cell.2004.08. ... cells in type II cells was as high as 60%. Notably, most Sca-1+ cells also expressed the type II cell marker Sp-C (Fig. 3 C). ...
... an integrative approach to the relevance of the membrane-aquaporin interaction to different processes related to plant cell ... Specific sites of protein-aquaporin interaction have been evaluated and new regulations of aquaporins described based on these ... Specific sites of protein-aquaporin interaction have been evaluated and new regulations of aquaporins described based on these ... Aquaporin isoforms heterotetramerizations are considered as novel regulatory mechanisms for plasma membrane (PIPs) and ...
Aquaporin 5 (x-ray structure at 2.0 Å resolution (PDB# 3D9S) is available) (Horsefield et al., 2008). Aqp5 is a marker for ... proliferation and migration of human breast cancer cells (Jung et al., 2011). Plays a role in chronic obstructive pulmonary ... 1] "The human aquaporin-5 gene. Molecular characterization and chromosomal localization." Lee 8621489. [2] "The ...
Flk-2 is a marker in hematopoietic stem cell differentiation: A simple method to isolate long-term stem cells Julie L. ... Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation - Nov 13, 2001 ... Cell Biology. *. You have access. Pattern formation in Escherichia coli: A model for the pole-to-pole oscillations of Min ... T cell infiltration of the prostate induced by androgen withdrawal in patients with prostate cancer Maria Mercader, Barbara K. ...
The cardiac progenitor cells for the outflow tract (OFT) reside in the visceral mesoderm and mesodermal core of the pharyngeal ... 18336735 - Expression of cd38 in human neuroblastoma sh-sy5y cells.. 8703015 - Adenovirus-mediated expression of aquaporin-5 in ... Labeled cells from the right SHF were found in the myocardium on the left dorsal side of the OFT, and cells from the left SHF ... Labeled cells from the right and left AHF migrated to regions of the ventral wall of the OFT close to the aortic and pulmonary ...
Small-cell carcinoma, thymoma. GABABR. Small-cell lung. GAD65. Rare thymoma, breast adenocarcinoma. mGluR1. Hodgkins lymphoma ... aquaporin-4; CASPR2, contactin-associated protein 2; DNER, δ-Notch-like epidermal growth factor-related receptor; DPPX, ... Small-cell carcinoma. Ganglionic AChR. adenocarcinomas. Amphiphysin. Small-cell carcinoma, breast adenocarcinoma. Muscle AChR. ... Small-cell carcinoma. LGI-1/CASPR2. Small-cell lung carcinoma, thymoma, or adenocarcinomas. ...
  • Aquaporin 5 (AQP5) may be involved in NSCLC by promoting lung‑cancer initiation and progression. (
  • The present study aimed to determine the role of AQP5 in migration and angiogenesis using NSCLC cells and HUVECs. (
  • AQPs 1, 3, 4, 5, 8 and 9 were screened in the NSCLC cell line H1299, and the present results showed that AQP5 mRNA was upregulated compared with the other AQP genes. (
  • At the protein level, AQP5 was significantly increased in H1299 cells compared with 16HBE cells. (
  • AQP5 knockdown in H1299 cells significantly decreased cell migration compared with untransfected cells, as demonstrated by both Transwell and wound closure assays. (
  • The supernatant of AQP5‑downregulated cells exhibited significantly low tube formation potential compared with untransfected cells. (
  • Similarly, vascular endothelial growth factor was significantly increased in control cells (si‑NC) compared with cells transfected with small interfering RNA targeting AQP5. (
  • The present study investigated the role of nitric oxide (NO)/cGMP signal transduction in the M 3 muscarinic acetylcholine receptor (mAChR)-stimulated increase in aquaporin-5 (AQP5) levels in the apical plasma membrane (APM) of rat parotid glands. (
  • Pretreatment of rat parotid tissue with the NO scavenger 2-(4carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide potassium inhibited both acetylcholine (ACh)- and pilocarpine-induced increases in AQP5 in the APM. (
  • A calmodulin kinase II inhibitor [(8)-5-isoquinolinesulfonic acid, 4-[2-(5-isoquinolinyl-sulfonyl)methylamino]-3-oxo-(4-phenyl-1-piperazinyl)-propyl]phenyl ester (KN-62)] decreased the pilocarpine-induced increase of AQP5 in the APM. (
  • Pretreatment of the tissues with a myosin light chain kinase inhibitor [(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine (ML-9)] inhibited a mAChR-stimulated increase in AQP5 levels in the APM. (
  • The site of action of Ca 2+ for the increase in AQP5 levels in the APM of rat parotid cells, however, is not known. (
  • Expression of aquaporin-5 (AQP5), studied by immunofluorescence and confocal microscopy, showed an increase in parallel with the increase in P f following hyperosmotic stress. (
  • The AQP5 was localized both in cytoplasmic vesicles and in apical cell membranes. (
  • 293, the kidney cell line, was used as a positive control for AQP5. (
  • We analyzed the prognostic impact of aquaporin 5 (AQP5) in non-small lung cancer (NSCLC). (
  • This may improve characterization of acute respiratory distress syndrome and ultimately facilitate individualized care.Background The aquaporin-5 (AQP5) -1364A/C promoter single-nucleotide polymorphism is associated with an alteredAQP5 expression and mortality in sepsis. (
  • Exosomes co-expressing AQP5-targeting miRNAs and IL-4 receptor-binding peptide inhibit the migration of human breast cancer cells. (
  • We assessed the distribution of aquaporin-5 (AQP5) in lacrimal gland biopsy samples. (
  • We show that the basolateral membrane aquaporin (AQP)-3, but not the equivalent apical membrane AQP5, is delivered in post-Golgi structures directly to forming cell-cell contacts where it co-accumulates precisely with E-cadherin. (
  • An EMT cell model was subsequently established by adding estradiol (E2) to RWPE-1 cells, after which AQP5 knockdown was performed. (
  • A newly identified genetic marker Aquaporin-5 (AQP5) was key to the landmark finding, which points the way to new therapeutic opportunities in future, including the development of regenerative medicine and improved stomach cancer treatments. (
  • Using the Lgr5 marker, the team found AQP5, a new surface marker that allowed them to isolate human stomach stem cells using antibodies, and functionally proved that they were stem cells. (
  • Using the new AQP5 marker to selectively introduce mutations to the stomach stem cells in mice, the researchers were able to highlight their role in driving the early formation of Wnt-driven gastric cancer. (
  • Importantly, they subsequently showed that stomach tumours harbour populations of AQP5-expressing cells that could behave as cancer stem cells to drive cancer growth. (
  • Our ability to identify and purify tumour-resident stem cells in gastric cancer using the new AQP5 marker will allow us to directly evaluate their role in human cancer formation, characterise them in depth and potentially develop drug-screening tests to identify ways to selectively kill these cells. (
  • Aquaporin 5 (AQP5) plays an important role in the salivary gland function. (
  • Ligation-reopening of main excretory duct induces changes in the AQP5 level which would give an insight for mechanism of regeneration/self-duplication of acinar cells. (
  • cAMP-dependent phosphorylation of AQP5 does not seem to be markedly involved in regulation of its intracellular trafficking but seems to play a role in its constitutive expression and lateral diffusion in the cell membrane. (
  • Publications] Matsuzaki T: 'Aquaporin-5(AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation. (
  • miR‑1271‑5p and aquaporin 5 (AQP5) expression at the mRNA level were measured by reverse transcription‑quantitative PCR (RT‑qPCR). (
  • The protein levels of AQP5, Bax, Bcl‑2, cleaved‑caspase-3 and proliferating cell nuclear antigen were quantified by western blot analysis. (
  • miR‑1271‑5p was downregulated, while AQP5 was upregulated in HBV‑related liver cancer cells and tissues. (
  • Overexpression of miR‑1271‑5p or AQP5 knockdown inhibited the levels of HBeAg, HBsAg and HBV DNA, blocked cell viability, migration and invasion, and induced apoptosis. (
  • Aquaporin 5 (Aqp5) is known to play a key role in salivary secretion, but the association between Aqp5 and the circadian rhythm is poorly understood. (
  • Triamcinolone acetonide inhibited the hyperosmotic expression of VEGF but not AQP5.The expression of AQP5 was decreased by hypoosmolarity, serum, and hypoxia.Hyperosmolarity induces the gene transcription of AQP5, AQP8, and VEGF, as well as the secretion of VEGF from RPE cells. (
  • Hyperosmolarity induces the gene transcription of AQP5, AQP8, and VEGF, as well as the secretion of VEGF from RPE cells. (
  • Hypoxia and hypoxia mimetics decrease aquaporin 5 (AQP5) expression through both hypoxia inducible factor-1? (
  • Aquaporin 5 antibody LS-C3810 is an unconjugated rabbit polyclonal antibody to Aquaporin 5 (AQP5) from mouse and rat. (
  • Aquaporin 5 (AQP5) expression impacts on cellular water transport, renal function but also on key mechanisms of inflammation and immune cell migration that prevail in sepsis and ARDS. (
  • The aquaporin 5 ( AQP5) -1364A/C promoter single nucleotide polymorphism affects key mechanisms of inflammation and immune cell migration. (
  • The results suggest that hyperosmotic stress is an important activator of AQP-5 in human airway epithelium, leading to significantly increased transepithelial water permeability. (
  • We computationally determined the diffusional (PD) and osmotic (Pf) water permeability coefficients for the mammalian CDE based on in silico simulations of cochlear water dynamics integrating previously derived in vivo experimental data on fluid flow with expression sites of molecular water channels (aquaporins, AQPs). (
  • Aquaporins (AQPs) are important in controlling water permeability. (
  • Due to the relatively high water permeability of some epithelial cells, it was long suspected that some additional mechanism for water transport across membranes must exist. (
  • Solomon and his co-workers performed pioneering work on water permeability across the cell membrane in the late 1950s. (
  • [5] In the presence of PKC, AQP1 was reported to have increased aqueous permeability. (
  • Heterotetramerizations of aquaporin isoforms are considered as novel regulatory mechanisms for plasma membrane (PIPs) and tonoplast (TIPs) proteins, influencing their intrinsic permeability and trafficking dynamics in the adaptive response to changing environmental conditions. (
  • Vasopressin-mediated control of water permeability in the renal collecting duct occurs in part through regulation of the distribution of aquaporin-2 (AQP2) between the apical plasma membrane and intracellular membrane compartments. (
  • Their balance in the cell determines water permeability of the plasma membrane. (
  • Thus multiple AQP4 mRNAs may allow long-term regulation of the balance between M1 and M23 AQP4 isoforms in the cell and thus water permeability of the plasma membrane. (
  • It provides these cells with a high permeability to WATER. (
  • Water channel proteins, known as aquaporins, are transmembrane proteins that mediate osmotic water permeability. (
  • Aquaporin-3 function is to promote glycerol permeability across cell membrane. (
  • Ishibashi K, Sasaki S, Fushimi K, Uchida S, Kuwahara M, Saito H, Furukawa T, Nakajima K, Yamaguchi Y, Gojobori T et al (1994) Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells. (
  • The analysis of the plasma membrane permeability of luminal epithelial cells by two separate cell swelling assays confirmed a highly increased water permeability of these cells in response to estrogen treatment. (
  • In the renal collecting duct, vasopressin regulates water permeability by a process that involves stimulation of adenylyl cyclase activity, cAMP production and subsequent translocation of water channel aquaporin-2 (AQP2) into the apical plasma membrane. (
  • Defective hepatocyte aquaporin-8 expression and reduced canalicular membrane water permeability in estrogen-induced cholestasis. (
  • Results show that changes in the amino acid composition of the aromatic/arginine region affect the size and polarity of the selectivity filter (SF) and could help explain the difference in water permeability between aquaporins. (
  • The osmotic and permeability parameters of a cell membrane are essential physico-chemical properties of a cell and particularly important with respect to cell volume changes and the regulation thereof. (
  • Presented here is a simple and very efficient method for the determination of the osmotic water permeability coefficient (P f ) in plant protoplasts, applicable in principle also to other spherical cells such as frog oocytes. (
  • Several aquaporins (AQPs), which form water channels that selectively transport water across the plasma membrane, have been cloned from a variety of mammalian tissues ( King and Agre, 1996 ). (
  • By reverse transcriptase-polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal cancer cell lines. (
  • In situ hybridization demonstrated that during colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease (early dysplasia) and maintained through the late stages of colon cancer development. (
  • Expression of AQPs 1 and 5 was maintained even in metastatic lesions in the liver. (
  • These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of colorectal cancer development. (
  • To test our hypothesis, we initially used reverse transcriptase-polymerase chain reaction (RT-PCR) to screen the expression profiles of human AQPs in seven colon and colorectal cancer cell lines, and confirmed our results by Western blot analysis. (
  • Of the five AQPs examined, AQPs 1, 3, and 5 were expressed in all the seven cell lines ( Figure 1a ). (
  • AQPs 2 and 8 were not expressed in any of these cell lines (data not shown). (
  • These data indicate that, although AQP3 is the only known AQP to be expressed in normal colonic surface epithelium, different AQPs seem to be expressed simultaneously in tumor cells. (
  • Western blot analysis demonstrated that AQPs 1, 3, and 5 were expressed concurrently in all four of the tested cancer cell lines ( Figure 1b ), confirming our RT-PCR results. (
  • NIH3T3 cells transfected with AQP1 and AQP3 expression constructs were used as positive controls for the Western blots of AQPs 1 and 3, respectively. (
  • Expression of AQPs 1, 3, and 5 in seven colon and colorectal cancer cell lines. (
  • Water channel proteins named aquaporins (AQPs) have been found in the cell membranes of these organisms. (
  • Among 13 AQPs, AQP4 has a characteristic ultrastructural feature, since AQP4 can be identified by freeze-fracture (F-F) electron microscopy and can be seen as orthogonal arrays (OAs) in this method [ 4 , 5 ]. (
  • Plants aquaporins (AQPs) are molecular proteinaceous membrane channels that finely control the passage of water through membranes. (
  • Aquaporins (AQPs) belong to a transmembrane protein family of water channels that are permeable to water by the osmotic gradient. (
  • AQPs may act as transmembrane osmosensors in red cells, secretory granules and microorganisms ( Hill and Shachar-Hill 2015 ). (
  • Aquaporins (AQPs) are integral membrane proteins that form pores in the membrane of biological cells. (
  • Various factors and channels, including vascular endothelial growth factor (VEGF) and aquaporins (AQPs), influence neovascularization and the development of edema. (
  • Therefore, we determined whether extracellular hyperosmolarity alters the expression of VEGF and AQPs in cultured human retinal pigment epithelial (RPE) cells. (
  • Aquaporins (AQPs) are membrane proteins that form water channels, allowing rapid movement of water across cell membranes. (
  • To gain insight into the mechanisms underlying this estrogen-stimulated water transport, we have explored the expression profile and functionality of water channels termed aquaporins (AQPs) in the ovariectomized mouse uterus treated with ovarian steroid hormones. (
  • The main role of salivary glands (SG) is the production and secretion of saliva, in which aquaporins (AQPs) play a key role by ensuring water flow. (
  • The AQPs are transmembrane channel proteins permeable to water to allow water transport across cell membranes according to osmotic gradient. (
  • Aquaporins are water channel proteins that play a major role in the movement of water in various human tissues. (
  • Many organisms lacking a specific aquaporin have an unobtrusive phenotype, which suggests that aquaporins are unimportant or can be substituted by homolog proteins or compensatory mechanisms. (
  • Aquaporins, also called water channels, are channel proteins from a larger family of major intrinsic proteins that form pores in the membrane of biological cells, mainly facilitating transport of water between cells. (
  • It was present in structures such as kidney tubules and red blood cells, and related to proteins of diverse origins, such as in fruit fly brain, bacteria, the lens of the eye, and plant tissue. (
  • Also known as water channels, aquaporins are integral membrane pore proteins. (
  • Aquaporins are integral membrane proteins that specialize in the regulation of cellular water flow across the cell membrane. (
  • AQP4 belongs to the aquaporin family of integral membrane proteins that conduct water through the cell membrane. (
  • In recent years, a number of studies have been focused on the structural evaluation of protein complexes in order to get mechanistic insights into how proteins communicate at the molecular level within the cell. (
  • Dr. Yun-Sil Lee and colleagues examined whether salivary glands could be protected by heat shock proteins (HSPs), which are expressed by cells in an effort to counteract environmental stressors such as heat, hypoxia, and ionizing radiation. (
  • Benga G (2009) Water channel proteins (later called aquaporins) and relatives: past, present, and future. (
  • The asymmetric distribution of membrane proteins in different cell surface domains is a feature common to many types of polarized cells, including epithelia, neurons, and immune cells ( Yamada and Nelson, 2007 ). (
  • In epithelial cells, membrane proteins are segregated into functionally and structurally different apical and basolateral membrane domains. (
  • In fully polarized cells, the delivery of basolateral membrane proteins from the TGN and recycling endosomes to the plasma membrane may be regulated at several steps, including long-range vesicle delivery and membrane tethering and fusion. (
  • apical and basolateral membrane proteins are intermixed on the cell surface, though they are sorted from each other in the exocytic pathway. (
  • basolateral membrane proteins are restricted to cell-cell contacts, whereas apical proteins accumulate on the unbounded membrane facing the growth medium. (
  • Aquaporins form a large, diverse family of proteins and have been found in bacteria, plants, and animals. (
  • Water is 'turned on' and 'turned off' by membrane proteins that function as water conduits and are called aquaporins. (
  • Previously unidentified phosphorylation sites were found for membrane proteins essential to collecting duct physiology, including eight sites among aquaporin-2 (AQP2), aquaporin-4, and urea transporter isoforms A1 and A3. (
  • and (6) AQP8s, or metazoan aquaporin 8 proteins. (
  • The cells in the spinous layer initiate the production of proteins characteristic of the differentiated epidermis. (
  • Corneocytes, which are the cells in the stratum corneum, contain extensively cross-linked proteins, surrounded by a highly resistant cell envelope. (
  • Aquaporins, channel proteins that facilitate the transfer of water across cell membranes, play a central role in maintaining the plant water status. (
  • Na transport proteins are expressed by rat alveolar epithelial type I cells Am J Physiol Lung Cell Mol Physiol. (
  • In order to evaluate the cell differentiation into male germ cells, immunocytochemistry was used to detect Mvh and Scp3 germ cell marker proteins. (
  • The levels of signaling proteins and nuclear factor of activated T cell 5 (NFAT5) were determined by western blotting. (
  • Aquaporins are channel producing proteins which regulate the flow of water across the cell membrane. (
  • Concomitantly, a rapid internalization of fluorescently-tagged PIP2;7 proteins was observed but removal from the cell membrane was not accompanied by further degradation of the protein within 4 h of exposure to salinity stress. (
  • Special interest in Aquaporins, Tight junction proteins. (
  • Background: Aquaporins are a large family of transmembrane channel proteins that are present throughout all domains of life and are implicated in human disorders. (
  • The effects of low root temperature on growth and root cell water transport were compared between wild-type Arabidopsis (Arabidopsis thaliana) and plants overexpressing plasma membrane intrinsic protein 1;4 (PIP1;4) and PIP2;5. (
  • The reduction of L(p) at 10°C in roots of wild-type plants was partly restored to the preexposure level by 5 mm Ca(NO(3))(2) and protein phosphatase inhibitors (75 nm okadaic acid or 1 μm Na(3)VO(4)), suggesting that aquaporin phosphorylation/dephosphorylation processes were involved in this response. (
  • M 3 mAChRs trigger similar signal transduction pathways that involve the heterotrimeric G protein Gq-mediated activation of phospholipase C (PLC) - β , which results in the generation of inositol 1,4,5-trisphosphate (IP 3 ) and 1,2-diacylglycerol (DAG). (
  • Arima H, Yamamoto N, Sobue K, Umenishi F, Tada T, Katsuya H, Asai K (2003) Hyperosmolar mannitol simulates expression of aquaporins 4 and 9 through a p38 mitogen-activated protein kinase-dependent pathway in rat astrocytes. (
  • Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. (
  • Sodium-glucose cotransporter 2 inhibitor use and reduced β‐cell function together resulted in insulinopenia and probably hyperglucagonem ia, which enhanced proteinolysis and impaired protein synthesis in the muscle, thereby leading to the patient developing sarcopenia. (
  • In situ immunological studies indicated NtAQP1 protein accumulation in the root exodermis and endodermis, in the cortex, close to the vascular bundles, in the xylem parenchyma, and in cells of the stomatal cavities. (
  • Aquaporin-1 is an integral membrane protein that is considered to have an "open" structure. (
  • Protein Kinase C (PKC) has been documented as a common signal transducer among the aquaporins. (
  • Benga G. The first discovered water channel protein, later called aquaporin 1: molecular characteristics, functions and medical implications. (
  • here we define aquaporin protein ontogeny in rat. (
  • Aquaporin-4, also known as AQP4, is a water channel protein encoded by the AQP4 gene in humans. (
  • Specific sites of protein-aquaporin interaction have been evaluated and new forms of regulation of aquaporins described, based on these associations. (
  • These epithelial cells express the stem cell marker Lgr6 (Leucine-rich repeat-containing G protein-coupled receptor 6). (
  • Reduced p38α promotes activation of Wnt signaling and, in human lung cancer samples, we observed a correlation between a deficiency in p38α protein and increase of Wnt signaling, promoting Lgr6 + cells during cancer progression. (
  • Mechanisms involved in maintaining plasma membrane domains in fully polarized epithelial cells are known, but when and how directed protein sorting and trafficking occur to initiate cell surface polarity are not. (
  • Functional disruption of individual components of a putative lateral targeting patch (e.g., microtubules, the exocyst, and soluble N -ethylmaleimide-sensitive factor attachment protein receptors) did not inhibit cell-cell adhesion or colocalization of the other components with E-cadherin, but each blocked AQP3 delivery to forming cell-cell contacts. (
  • Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry. (
  • In contrast, another heat shock protein, hsp70-5 (BiP/grp78), bound to phosphorylated AQP2 more avidly than to nonphosphorylated AQP2. (
  • Adult stem cells, marked by the Lgr5 protein, are responsible for the continuous regeneration and replacement of tissues in many organs, but can also cause cancer if damaged. (
  • Investigation of the effects of aging on the expression of aquaporin 1 and aquaporin 4 protein in heart tissue. (
  • Mice with targeted disruption of the acyl-CoA binding protein display attenuated urine concentrating ability and diminished renal aquaporin-3 abundance. (
  • This year, Roderick MacKinnon was recognized for working out the atomic structure of an ion channel and Peter Agre for discovering that a major protein found in red blood cells functions primarily as a water channel. (
  • The aquaporin Z channel protein in E. coli can accommodate a flow of water at rates six times higher than GlpF (aquaglyceroporin glycerol facilitator, a channel protein that transports both glycerol and water in E. coli) making it the prime subject for studying the selectivity of a high-conducting water channel. (
  • This study reveals the practicality of liquid chromotography-mass spectrometry n neutral loss scanning for large-scale identification and quantification of protein phosphorylation in the analysis of cell signaling in a native mammalian system. (
  • The method is used here for analysis of protein phosphorylation in vasopressin-sensitive inner medullary collecting duct (IMCD) cells freshly isolated from rat kidneys. (
  • The Major Intrinsic Protein (MIP) of the human lens of the eye (Aqp0), after which the MIP family was named, represents about 60% of the protein in the lens cell. (
  • Protein kinases involved in water transport regulation are used to explore fundamental properties of plant cell signaling pathways. (
  • Alveolar epithelial type I cells express beta2-adrenergic receptors and G-protein receptor kinase 2 J Histochem Cytochem. (
  • I was a Wellcome fellow at Birmingham before joining Aston University working on G protein coupled receptors (GPCRs) and Aquaporins. (
  • the aquaporin water channels and the G-protein coupled receptors (GPCRs). (
  • Understanding the yeast host cell response to recombinant membrane protein production. (
  • Membrane trafficking of aquaporin 1 is mediated by protein kinase C via microtubules and regulated by tonicity. (
  • Immunofluorescent staining was applied to determine the expression of AECII cell marker surfactant protein C (SPC) and AECI cell marker aquaporin-5 (AQP-5). (
  • Caption: FIGURE 1: Immunofluorescence and confocal images of hepatitis B virus core antigen (HBcAg) and hepatitis B virus x protein (HBx) and the stem cell marker epithelial cell adhesion molecule (EpCAM) in human hepatocellular carcinoma (HCC) and adjacent nontumor tissues. (
  • B. Release of VEGF protein from RPE cells measured under hyperosmotic conditions. (
  • HT-29 human colonic epithelial cells were treated with IFNγ to assess AQP3 mRNA expression by real-time RT-PCR and functional protein expression through the uptake of radiolabelled glycerol. (
  • IFNγ decreased AQP3 mRNA expression in HT-29 cells in a time- and concentration-dependent manner and reduced functional AQP3 protein expression (decreased 3 H-labelled glycerol uptake). (
  • Ramirez-Lorca R, Vizuete ML, Venero JL, Revuelta M, Cano J, Ilundain AA, Echevarria M (1999) Localization of aquaporin-3 mRNA and protein along the gastrointestinal tract of Wistar rats. (
  • Prolactin-induced protein (PIP) regulates proliferation of luminal A type breast cancer cells in an estrogen-independent manner PLoS One. (
  • Here, we demonstrate that high intracellular CO 2 /HCO 3 − enhances currents mediated by the Arabidopsis thaliana guard cell S-type anion channel SLAC1 upon coexpression of any one of the Arabidopsis protein kinases OST1, CPK6, or CPK23 in Xenopus laevis oocytes. (
  • Boursiac Y, Boudet J, Postaire O, Luu DT, Tournaire-Roux C, Maurel C (2008) Stimulus-induced downregulation of root water transport involves reactive oxygen species-activated cell signalling and plasma membrane intrinsic protein internalization. (
  • We have previously shown that in cos 1 cells in vitro, both adenylyl cyclase activity and cAMP production can be regulated by VACM-1, a cul 5 gene that forms complexes involved in protein ubiquitination and subsequent degradation. (
  • Immunocytochemistry and Western blot analyses data indicate that VACM-1/cul5 expression in MDCK line stably expressing AQP2 gene and in cos 1 cells co-transfected with the AQP2 and VACM-1/cul5 cDNAs decreased AQP2 protein concentration when compared to the vector transfected control groups. (
  • Compare SOCE current before and after oxidative stress, use pharmacological blockers of the relevant signaling components such as STIM1 (an endoplasmic reticulum Ca 2+ sensor), Orai1 (a cell membrane Ca 2+ pore controlled by STIM1), TRPM2 (a Ca 2+ channel that is redox-sensitive) and aquaporin 5 (a water channel) to assess the role of each protein in the observed stress-induced changes. (
  • Western blot on cell lines: compare protein expression levels of the same signaling components. (
  • Intermediate-risk patients (previously, submas- high risk for high-risk respond to hor- www.Uspreventiveservicestaskforce.Org/page/document/ mone therapy for acute pain can be quite thin and develop a significant and paranoia, auditory hallucinations, which are all cell cycle control quantity of protein greater than 2 cm in length is ideal), because the diagnosis can be. (
  • Comparative Analysis for the Presence of IgG Anti-Aquaporin-1 in Patients with NMO-Spectrum Disorders. (
  • Anti-Aquaporin 9 antibody , (1:200). (
  • Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen. (
  • Immunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 antibody (1:100). (
  • Immunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 antibody , (1:100). (
  • In this context, the coordination of local molecular and cellular events in the microenvironment of niches play pivotal roles in maintaining the balance of stem and differentiated cells for injury repair and regeneration in lung (Figure 1 ) [ 4 , 9 , 29 - 31 ]. (
  • These results provide insights into the molecular mechanisms of salt and water secretion by lung submucosal glands, and they suggest that while murine submucosal gland fluid secretion in response to cholinergic stimulation can originate from CFTR-expressing serous acinar cells, it is not dependent upon CFTR function. (
  • The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes). (
  • The molecular driving forces stabilizing the positions of the lipids around aquaporins could define their activity, thereby altering the conformational properties. (
  • And because the two main classes of aquaporins occur in E. coli--which means they're exposed to the same cellular environment--the opportunities for comparative structural and functional analyses, combined with site-directed mutagenesis, promise to provide valuable insights into the molecular underpinnings of the selectivity of functionally different aquaporins. (
  • With two structural models of aquaporins down to the atomic level in the same species, scientists can now begin to investigate the molecular mechanisms that facilitate their selectivity. (
  • This gave us an opportunity to compare open and closed aquaporins and to understand how this opening and closing works at the molecular level. (
  • The molecular and cellular bases of this process are addressed by studying the multiple facets of aquaporin regulation. (
  • The team combines molecular, physiological and genetic approaches and characterizes water transport at the level of cloned aquaporins, purified membranes, living cells or organs like excised roots or rosettes. (
  • Identification and molecular mechanisms of the rapid tonicity-induced relocalization of aquaporin 4. (
  • Agre P, Kozono D. Aquaporin water channels: molecular mechanisms for human diseases. (
  • Molecular and cellular features of aquaporin expression. (
  • BBA Molecular Cell Research 1865 (2018) 1771-1777). (
  • Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis J Clin Invest. (
  • Rat aquaporin-5 43-kb 5'-flanking region differentially regulates expression in salivary gland and lung in vivo. (
  • Rapid aquaporin translocation regulates cellular water flow: the mechanism of hypotonicity-induced sub-cellular localization of the aquaporin 1 water channel. (
  • Aquaporin-4 regulates water balance in the central nervous system. (
  • Aquaporin 5 regulates cigarette smoke induced emphysema by modulating barrier and immune properties of the epithelium. (
  • Taken together, these observations indicate that CARM1 regulates cell cycle progression and cellular growth in response to steroids. (
  • Vasopressin-Independent Regulation of Aquaporin-2 by Tamoxifen in Kidney Collecting Ducts. (
  • Regulation of Plasma Membrane Nanodomains of the Water Channel Aquaporin-3 Revealed by Fixed and Live Photoactivated Localization Microscopy. (
  • Aquaporin channels may be subject to intense short term regulation via signal transduction. (
  • We investigated the role of keratinocyte growth factor (KGF) in regulation of alveolar epithelial cell (AEC) phenotype in vitro. (
  • Other performances that aquaporin-4 is involved in are synaptic plasticity, astrocyte migration, regulation of extracellular space volume, and the homeostasis of potassium. (
  • Elucidation of the epigenetic basis for cell-type specific gene regulation is key to gaining a full understanding of how the distinct phenotypes of differentiated cells are achieved and maintained. (
  • Aquaporin 1 and 5 expression decreases during human intervertebral disc degeneration: novel HIF-1-mediated regulation of aquaporins in NP cells. (
  • Conner AC, Bill RM and Conner MT. Aquaporin Regulation. (
  • The female TGF beta RI-coko mice also showed an increase in Th1 proinflammatory cytokines in salivary glands and exhibited an up-regulation of peripheral T cells. (
  • Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: involvement of NFAT5. (
  • Alexandersson E, Danielson JA, Rade J, Moparthi VK, Fontes M, Kjellbom P, Johanson U (2010) Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress. (
  • Aroca R, Ferrante A, Vernieri P, Chrispeels MJ (2006) Drought, abscisic acid and transpiration rate effects on the regulation of PIP Aquaporin Gene expression and abundance in Phaseolus vulgaris plants. (
  • According to the position within the airway tree, several epithelial cell types in the adult lung have been suggested to act as stem/progenitor cells in response to injury and exert the role in the local injury repair [ 1 , 4 , 9 ]. (
  • The Na + /H + exchanger NHE1 localizes as clusters to cryptic lamellipodia and accelerates collective epithelial cell migration. (
  • In these specific cases of epithelial cell expression, AQP4 is concentrated within the basolateral membrane layer of these locations. (
  • We tested whether establishment of the basolateral membrane domain and E-cadherin-mediated epithelial cell-cell adhesion are mechanistically linked. (
  • Cell adhesion to other cells and the extracellular matrix is important in the generation of epithelial cell surface polarity. (
  • Excessive cell sloughing resulted in fewer epithelial cell layers in spite of increased cell proliferation at the Klf4 CN ocular surface. (
  • Modulation of ion conductance and active transport by TGF-beta 1 in alveolar epithelial cell monolayers Am J Physiol Lung Cell Mol Physiol. (
  • An understanding of the process of alveolar epithelial cell growth and differentiation requires the ability to trace and analyze the phenotypic transitions that the cells undergo. (
  • Nanoparticle translocation across mouse alveolar epithelial cell monolayers: species-specific mechanisms. (
  • In order to maintain its homeostasis and normal functions following injuries caused by varied extraneous and intraneous insults, such as inhaled environmental pollutants and overwhelming inflammatory responses, the respiratory epithelium normally undergoes regenerations by the proliferation and differentiation of region-specific epithelial stem/progenitor cells that resided in distinct niches along the airway tree. (
  • Studies using cell differentiating and lineage tracing assays, in vitro and/or ex vivo models, and genetically engineered mice have suggested that these local epithelial stem/progenitor cells within spatially distinct regions along the pulmonary tree contribute to the injury repair of epithelium adjacent to their respective niches. (
  • The alveolar epithelium is composed of the flat type I cells comprising 95% of the gas-exchange surface area and cuboidal type II cells comprising the rest. (
  • In the airway epithelium, specific cells act as progenitor cells to repair different regions of airways. (
  • The lung alveolar epithelium is composed of type I cells that make up 95% of the alveolar surface area and type II cells making up the remainder ( Mason, 2006 ). (
  • Furthermore, AQP4 also plays a role in the supportive cells of sensory organs, such as the retina, inner ear, and olfactory epithelium. (
  • Within the inner ear, the main role is to provide osmotic balance in supporting epithelium cells within the organ of Corti by recycling K+. (
  • In the mouse, following eye opening around 2 weeks after birth, cells in the one- to two-cell-layered epithelium proliferate and differentiate to form a four- to five-cell-layered stratified squamous epithelium by 20 days after birth ( 74 ). (
  • The mature corneal epithelium containing five to eight cell layers is present by 6 to 8 weeks after birth ( 15 , 30 ). (
  • Aquaporin 9 (brown staining) is expressed in the columnar epithelium of the epididymus (arrows). (
  • [4] It is also indicated in proper lung development by controlling the late differentiation stages of alveolar epithelium and aquaporin-5 promoter activation. (
  • Int J Biochem Cell Biol. (
  • Knockout Mice Reveal a Major Role for Alveolar Epithelial Type I Cells in Alveolar Fluid Clearance Am J Respir Cell Mol Biol. (
  • Knockout mice reveal key roles for claudin 18 in alveolar barrier properties and fluid homeostasis Am J Respir Cell Mol Biol. (
  • Identification of three genes of known function expressed by alveolar epithelial type I cells Am J Respir Cell Mol Biol. (
  • Estrogens and selective estrogen receptor modulators differentially antagonize Runx2 in ST2 mesenchymal progenitor cells J Steroid Biochem Mol Biol. (
  • J Cell Biol (2007) 178 (2): 323-335. (
  • In mammals, they have been identified in cell membranes such as in epithelial and endothelial cells. (
  • The presence of OAs in cytoplasmic half (P face) of frozen cleaved biological plasma membranes has been described in different cell types of different species and different organs and tissues. (
  • The cell membranes of a variety of different bacteria, fungi, animal and plant cells contain aquaporins through which water can flow more rapidly into and out of the cell than by diffusing through the phospholipid bilayer. (
  • In most cells, water moves in and out by osmosis through the lipid component of cell membranes. (
  • Aquaporin-1 (AQP1) was first discovered in human red blood cell membranes by Gheorghe Benga's research group in 1986. (
  • Extensive research on the function of aquaporins have been implemented into many separate types of cell membranes. (
  • Osmotic movement of water across bacterial cell membranes is postulated to be a homeostatic mechanism for maintaining cell turgor. (
  • AQP4 staining in vivo normally is polarized in the astrocytic endfoot membranes at the glia limitans superficialis and perivascularis, but in C6 and RG2 tumors the AQP4 staining is redistributed over the whole glioma cell as in human glioblastoma. (
  • Western blot analysis of rat brain (lanes 1and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes: 1,2,3. (
  • These channels, allow the passive but selective movement of water and other small neutral solutes across cell membranes. (
  • P-glycoprotein (P-gp), a kind of ATP-binding cassette transporter, can export candidates through a channel at the two transmembrane domains (TMDs) across the cell membranes using the energy released from ATP hydrolysis at the two nucleotide-binding domains (NBDs). (
  • Cohen DM, Wasserman JC, Gullans SR (1991) Immediate early gene and HSP70 expression in hyperosmotic stress in MDCK cells. (
  • Here, submucosal gland serous acinar cells were isolated from murine airway, identified by immunofluorescence and gene expression profiling, and used in physiological studies. (
  • The human Aquaporin-5 gene. (
  • Mechanistic investigations suggested that a defective repression of the miR-17-92 gene cluster was responsible for evolution of a selection for outgrowth of Lgr6 + NSCLC cells. (
  • Journal Article] g-tocotrienol enhances the chemosensitivity of human oral cancer cells to docetaxel through the downregulation of the expression of NF-kB-regulated anti-apoptotic gene products. (
  • Less than a decade ago, scientists discovered the aquaporin Z gene (aqpZ) in E. coli, pointing to the protein's role in regulating water transport in this prokaryote. (
  • The 5′-untranslated region (5′-UTR) of M23A mRNA is encoded by four new exons (A, B, C, and D), which are located in the 5′ region from exon-0 of the AQP4 gene. (
  • Introduction of exogenous genes, followed by three-dimonsional confocal image analyses proved to be a very convenient and powerful tool in the identification of gene product expressed in the cells. (
  • Subunit-specific coordinate upregulation of sodium pump activity in alveolar epithelial cells by lentivirus-mediated gene transfer Hum Gene Ther. (
  • In addition, gene expression analysis by qRT-PCR revealed similar mRNA levels of the pluripotent stem cell marker OCT-4 for exposed and non-exposed cells (Figure 3A). (
  • We are a basic science lab, interested in transcriptional control (in particular combinatorial transcriptional control of mammalian gene expression), steroid hormone signaling, and cell biology (including stem cells and cell cycle control). (
  • Aquaporin-2 levels in vitro and in vivo are regulated by VACM-1, a cul 5 gene. (
  • Gene expression analysis reveals a dysregulation of cell cycle genes and markers of differentiation in the Carm1 knockout lung. (
  • Furthermore, there is an overlap in gene expression in the Carm1 knockout and the glucocorticoid receptor knockout lung, suggesting that hyperproliferation and lack of maturation of the alveolar cells are at least in part caused by attenuation of glucocorticoid-mediated signaling. (
  • A limited number of aquaporins are found within the central nervous system (CNS): AQP1, 3, 4, 5, 8, 9, and 11, but more exclusive representation of AQP1, 4, and 9 are found in the brain and spinal cord. (
  • In the mid nineties of the last century, the OAs turned out to be a water channel named aquaporin 4 (AQP4). (
  • In the CNS, AQP4 is the most prevalent aquaporin channel, specifically located at the perimicrovessel astrocyte foot processes, glia limitans, and ependyma. (
  • The assembly of AQP4 monomers into tetramers is similar to other aquaporin channels. (
  • Additionally, AQP4 can also be located in epithelial cells of many organs throughout the human body, such as the kidney, intestine, salivary glands, sensory organs, and skeletal muscles. (
  • Within the retina, AQP4 is highly concentrated where the processes of Muller cells have a basal lamina around blood vessels and inner limiting membrane and to a lesser degree in the inner and outer plexiform layers. (
  • Neuromyelitis optica (NMO) is an autoimmune CNS disorder mediated by pathogenic aquaporin-4 (AQP4) water channel autoantibodies (AQP4-IgG). (
  • We identified a group of AQP4 rAbs targeting a distinct extracellular loop C epitope that demonstrated enhanced CDC on target cells. (
  • [5] Within the retina, AQP4 is highly concentrated where the processes of Muller cells have a basal lamina around blood vessels and inner limiting membrane. (
  • [11] Aquaporin-4 is the predominant autoimmune target in neuromyelitis optica, or NMO, since a specific AQP4 IgG autoantibody, or NMO-IgG, binds to the extracellular surface of AQP4. (
  • Aquaporin-4 (AQP4), the main water channel of the brain, is highly expressed in animal glioma and human glioblastoma in situ. (
  • In contrast, most cultivated glioma cell lines don't express AQP4, and primary cell cultures of human glioblastoma lose it during the first passages. (
  • Accordingly, in C6 cells and RG2 cells, two glioma cell lines of the rat, and in SMA mouse glioma cell lines, we found no AQP4 expression. (
  • We confirmed an AQP4 loss in primary human glioblastoma cell cultures after a few passages. (
  • RG-2 glioma cells if grafted into the brain developed AQP4 expression. (
  • In this study, we showed for the first time implanted AQP4 negative glioma cells in animal brain or flank to express AQP4 specifically in the intracerebral gliomas but neither in the extracranial nor in the flank gliomas. (
  • Her presenterer vi en protokoll for å indusere lammelse og opticospinal betennelse av overføring av aquaporin-4 (AQP4)-spesifikke T-celler fra AQP4 - / - mus i WT mus. (
  • Quantification of phosphopeptides in this setting has been challenging and has been successful so far in cultured cells ( 5 ) and yeast ( 6 ) but not in native mammalian cells and tissues. (
  • Initial Characterization of Osteoblast Differentiation and Loss of RUNX2 Stability in the Newly Established SK11 Human Embryonic Stem Cell-Derived Cell Line J Cell Physiol. (
  • Non‑small cell lung cancer (NSCLC) constitutes the majority of all lung‑cancer cases. (
  • Lung is a complex organ lined with epithelial cells. (
  • The importance of local epithelial stem cell niches in the specification of lung stem/progenitor cells has been recently identified. (
  • This paper reviews recent findings in the identification and isolation of region-specific epithelial stem/progenitor cells and local niches along the airway tree and the potential link of epithelial stem cells for the development of lung cancer. (
  • In order to meet the need of regional functionalities of the lung, the epithelia within each domain of the conducting airway are properly composed with distinct types of epithelial cells. (
  • Similar to other adult tissues and organs, epithelial stem/progenitor cells in adult lung are a subset of undifferentiated cells that undergo asymmetric cell division during normal lung morphogenesis and possibly regeneration [ 8 ]. (
  • In terms of the potential stem cell niches in lung, studies using murine models have revealed several unique regional niches for distinct epithelial stem/progenitor cell populations along the proximal-distal axis of airway, along which the epithelial stem cells reside in their specific local niches in order to maintain tissue homeostasis during injury repair and normal turnover. (
  • Some exhibit a high rate of constant cell turnover, while others, such as the lung, exhibit only low-level cell regeneration during normal adult homeostasis but have the ability to rapidly regenerate new cells after injury. (
  • Lung regeneration likely involves both activation of progenitor cells as well as cell replacement through proliferation of remaining undamaged cells. (
  • The lung is extremely complex, and both development and repair require interaction among more than 40 different cell lineages (for review see refs. (
  • The complex spatial organization of the lung and the sheer variety of cells that constitute the mature organ dictate that repair after injury may require multiple pools of progenitor cells capable of self renewal and multipotency. (
  • Therefore, an improved understanding of both developmental biology and progenitor/stem cell biology, as it pertains to the mature lung and the generation of various cell types within the alveolar and bronchiolar airways, is paramount to the development of better therapies for both acute and chronic lung disease. (
  • In this study, we tested the effect of administering MSC directly into the airspaces of the lung 4 h after the intrapulmonary administration of Escherichia coli endotoxin (5 mg/kg). (
  • The beneficial effect of MSC was independent of the ability of the cells to engraft in the lung and was not related to clearance of the endotoxin by the MSC. (
  • Recent studies have demonstrated that bone marrow-derived mesenchymal stem cells (MSC) have the ability to participate in the repair of experimental models of bleomycin-induced lung injury ( 3 , 4 ). (
  • Despite the low levels of MSC incorporation into the lung, the administration of these cells does attenuate the severity of bleomycin-induced lung injury ( 3 , 4 ), implying that the cells are acting in a manner independent of their ability to engraft. (
  • Thus, expression of FoxM1 in type II cells is essential for their proliferation and transition into type I cells and for restoring alveolar barrier homeostasis after PA-induced lung injury. (
  • Thus, FoxM1 is required for the progenitor cell function of type II epithelial cells and repairing of the alveolar epithelial barrier after PA-induced lung injury. (
  • Here we report that the lung stem cell marker Lgr6 becomes enriched in non-small cell lung cancer (NSCLC) cells during malignant progression. (
  • However, therapies applied using drugs directed to those molecules or related pathways have failed to significantly increase survival, especially in the most prevalent cancer types, for example, lung adenocarcinoma ( 3-5 ). (
  • Previously, efforts have focused on investigating the cells and mechanisms involved in the resistance and later virulence of lung cancers after therapeutic treatments ( 7, 8 ). (
  • We have previously characterized a population of human lung cells with stem cell potential to differentiate into all bronchioalveolar lineages while being able to self-renew. (
  • We have analyzed the contribution of Lgr6 + cells to human lung adenocarcinomas. (
  • We have observed that cells-expressing Lgr6 are enriched from early to late stages of human lung adenocarcinomas malignancy. (
  • We demonstrate how a novel-specific population of cells, and their regulatory mechanisms, is linked and contribute to better understanding of the malignant progression of lung adenocarcinomas. (
  • Expression of aquaporins in bronchial tissue and lung parenchyma of patients with chronic obstructive pulmonary disease. (
  • Regulatory T cell DNA methyltransferase inhibition accelerates resolution of lung inflammation. (
  • Foxp3+ regulatory T cells promote lung epithelial proliferation. (
  • Immunological priming requires regulatory T cells and IL-10-producing macrophages to accelerate resolution from severe lung inflammation. (
  • This model recapitulates an in vivo process in which AEC transition from one differentiated cell type to another during regeneration following lung injury. (
  • In addition to lung transplantation, Dr. Liebler research interests also include lung injury and repair and cell-based treatment of lung diseases. (
  • Region-specific role for Pten in maintenance of epithelial phenotype and integrity Am J Physiol Lung Cell Mol Physiol. (
  • Combinations of differentiation markers distinguish subpopulations of alveolar epithelial cells in adult lung Am J Physiol Lung Cell Mol Physiol. (
  • Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injury Am J Physiol Lung Cell Mol Physiol. (
  • Non-small cell lung cancer (NSCLC) accounts for 75-80% of all lung cancers. (
  • We used a lung injury model in mice to identify and characterize functional lung stem cells to clarify how stem cell niches counteract this degenerative process. (
  • Short term assay (STA) - Bleomycin-induced lung inflammation and fibrosis were assessed in a model of idiopathic pulmonary fibrosis in wild-type (WT), gp91phox-/- (NOX-/-), and gp91phoxMMP-12 double knockout (DKO) mice on C57Bl/6 background and Hoechst 33322 dye effluxing side population (SP) cells characterized. (
  • Long term assay (LTA) - In a bleomycin induced lung fibrosis model in C57Bl6 mice, the number of mature cells were quantified over 7, 14, and 21 days in bone marrow (BM), peripheral blood (PB), lung parenchyma (LP) and brochoalveolar lavage (BAL) fluid by FACS. (
  • STA - In lung, cellularity increased by 5-fold in WT and 6-fold in NOX-/- by d7. (
  • Many of these processes are ablative in nature and require repair mechanisms that regenerate mature lung tissue through cell proliferation and differentiation. (
  • Upon the disruption of GATA-6 in an embryo, the distal lung epithelial development is stunted in transgenic mice models [4] The progenitor cells , or stem cells, for alveolar epithelial tissues develop and are specified appropriately, however further differentiation does not occur. (
  • To this end, monoclonal antibodies have been generated to type I alveolar epithelial cells using an approach designed to enhance production of lung-specific clones from a crude lung membrane preparation. (
  • Combinations of differentiation markers distinguish subpopulations of alveolar epithelial cells in adult lung. (
  • Since then, 12 other isoforms of aquaporin-1 have been discovered and each have been classified under the family known as Aquaporins. (
  • Now there are at least 12 isoforms of Aquaporins are cloned. (
  • Water moves through cells in an organized way, most rapidly in tissues that have aquaporin water channels. (
  • Aquaporins in complex tissues. (
  • These cancer stem cells would be responsible for tumor maintenance and would harbor the potential to colonize other tissues as metastatic cells ( 11, 12 ). (
  • This coadjuvant process, in parallel to oncogenic mutations, helps select cells with a higher potential to perpetuate (self-renewal) and manipulate the microenvironment, facilitating colonization of foreign tissues (metastasis). (
  • An international team has, for the first time, identified and isolated human stomach stem cells that are responsible for renewing stomach tissues. (
  • Although Lgr5 is a selective marker of stomach stem cells in the mouse, researchers could not previously identify the equivalent Lgr5-expressing stem cells from human tissues due to the lack of surface markers that enable its isolation and validation. (
  • Furthermore, as Lgr5 is expressed across a diverse range of tissues including the intestine, stomach and kidney, the team was unable to target cancer-causing mutations specific to the stomach stem cells to evaluate their role in stomach cancer. (
  • 8 It is well known that dendritic cells (DCs), a rare population of leukocytes that are distributed throughout the lymphoid and nonlymphoid tissues in the entire body, are important for the initiation of acquired immunity by stimulating naïve T cells as APCs. (
  • The Aquaporin team studies the various modes of water transport in plant tissues. (
  • The goal of regenerative medicine is to aid repair and regeneration of the lost tissues in parenchyma and airways for which mobilization of tissue-resident endogenous or bone marrow-derived exogenous stem cells niches is a critical step. (
  • Plant stomata, which are formed by pairs of guard cells in the epidermis of aerial tissues, control gas exchange and transpiration. (
  • The epithelial sodium channel (ENaC) is expressed in epithelial cells of several tissues involved in salt and water reabsorption. (
  • Localization of aquaporin 5 was clear demonstrated in these complex acinus and duct systems. (
  • Nuclear fluorescent counterstaining is also useful in determining the localization of channels and transporters in cultured epithelial cells. (
  • Foxj1 is required for apical localization of ezrin in airway epithelial cells J Cell Sci. (
  • This localization implicates all four isotypes in movement of water during uterine imbibition and, based on their localization to the luminal epithelial cells, AQP-2 and -3 in facilitating water movement into the lumen of the uterus. (
  • Altered aquaporin 9 expression and localization in human hepatocellular carcinoma. (
  • RESULTS: Fluconazole increased plasma membrane localization of AQP2 in principal cells independent of AVP. (
  • Identification of new M23A mRNA of mouse aquaporin-4 expressed in brain, liver, and kidney by T. Yu. (
  • Analysis of mRNA 5′-UTR structure showed low translation efficacy for M1 mRNA in comparison with high translation efficacy for M23A, M23X, and M23 mRNAs. (
  • A subgroup of aquaporins called aquaglycerporins allow the passage of small solutes such as glycerol, urea, and ammonia. (
  • Boury-Jamot M, Sougrat R, Tailhardat M, Le Varlet B, Bonte F, Dumas M, Verbavatz JM (2006) Expression and function of aquaporins in human skin: Is aquaporin-3 just a glycerol transporter? (
  • The known aquaporins cluster loosely together as do the known glycerol facilitators. (
  • Aquaporins selectively transport water (but not glycerol) while glycerol facilitators selectively transport glycerol but not water. (
  • Aquaporins are selective water/glycerol channels involved in the maintenance of volume homeostasis and ionic/osmotic balance. (
  • Aquaporins have been classified into two subfamilies: i) strict aquaporins that only allow the passage of water and ii) the less selective aquaglyceroporins that transport water and other neutral solutes, such as glycerol, CO2 or urea. (
  • These cells would also be especially resistant to drugs, and they would be selected during tumor progression, generating more aggressive and metastatic disease ( 13, 14 ). (
  • By closing these aquaporins, tumor growth and metastases could be inhibited. (
  • However, the tumor was negative for lymphoid markers, the B cell marker UCHL-1, and the T cell marker L26. (
  • Solid tumor cells show a resistance to immunologic effector cells in vitro[1]. (
  • Dendritic cells play an important role in the immune response to tumor-associated antigens in humans. (
  • Aono S, Hirai Y (2008) Phosphorylation of claudin-4 is required for tight junction formation in a human keratinocyte cell line. (
  • We carried out phosphoproteomic analysis of rat inner medullary collecting duct cells by using a combination of phosphopeptide enrichment by immobilized metal affinity chromatography and phosphorylation site identification by liquid chromatography-mass spectrometry n neutral loss scanning. (
  • In this study, using cultures of rat primary pulmonary vascular smooth muscle cells (PASMC), we found that hypoxia induced strongly STAT3 phosphorylation by up to four-fold. (
  • Genetic defects involving aquaporin genes have been associated with several human diseases including nephrogenic diabetes insipidus and neuromyelitis optica. (
  • Expression of the keratin-12 and aquaporin-5 genes was downregulated, consistent with the Klf4 CN corneal epithelial fragility and stromal edema, respectively. (
  • For the introduction of exogenous genes into cells, we used microinjection and lipofection methods We transiently expressed GLUT4 glucose transporter in cultured cells, and its immunolocalization and translocation to the plasma membrane was monitored with conventional and confocal fluorescence microscopes. (
  • In RPE cells, high osmolality induces expression and secretion of angiogenic factors, such as vascular endothelial growth factor (VEGF), placental growth factor, and basic fibroblast growth factor, and expression of aquaporin-5, a water channel implicated in transepithelial water transport. (
  • Rodrigues O , Reshetnyak G , Grondin A , Saijo Y, Leonhardt N, Maurel C , Verdoucq L ✉ (2017) Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure . (
  • With characteristics of stem/progenitor cells, this subpopulation of cells possesses a capacity of self-renewal, proliferation, and differentiation both in a steady state and in response to injury in the physiologic domain of which they reside. (
  • In mice lacking FoxM1 specifically in type II cells, type II cells showed decreased proliferation and impaired trans-differentiation into type I cells. (
  • we found that FoxM1 expression in type II cells after PA challenge was coupled to increased type II cell proliferation and reannealing of the alveolar epithelial barrier. (
  • Inactivation of FoxM1 in type II alveolar cells in mice prevented type II cell proliferation and their trans-differentiation into type I cells, resulting in prolonged alveolar barrier dysfunction. (
  • Furthermore, GATA-6 has been linked to the production of LIF, a cytokine that encourages proliferation of endodermal embryonic stem cells and blocks early epiblast differentiation. (
  • In adults, CARM1 is overexpressed in human grade-III breast tumors and prostate adenocarcinomas, and knockdown of CARM1 inhibits proliferation of breast and prostate cancer cell lines. (
  • Based on these observations, we hypothesized that loss of CARM1 in mouse embryos would inhibit pulmonary cell proliferation, resulting in respiratory distress. (
  • In the absence of CARM1, alveolar type II cells show increased proliferation. (
  • These results demonstrate for the first time that CARM1 inhibits pulmonary cell proliferation and is required for proper differentiation of alveolar cells. (
  • CARM1 has also been implicated in cancer cell proliferation. (
  • In estrogen-treated MCF-7 human breast cancer cells, CARM1 knockdown results in reduced cellular proliferation and cell cycle progression. (
  • Effects of transdifferentiation and EGF on claudin isoform expression in alveolar epithelial cells J Appl Physiol (1985). (
  • Estrogens and androgens inhibit association of RANKL with the pre-osteoblast membrane through post-translational mechanisms J Cell Physiol. (
  • Glucocorticoids Hijack Runx2 to Stimulate Wif1 for Suppression of Osteoblast Growth and Differentiation J Cell Physiol. (
  • Dependence of castration-resistant prostate cancer (CRPC) stem cells on CRPC-associated fibroblasts J Cell Physiol. (
  • Cell Physiol Biochem. (
  • J Cell Physiol 184:409-20. (
  • 2018 Dec 5;13(12):e0208582. (
  • Cytoprotective effect of aquaporin 1 against lipopolysaccharide-induced apoptosis and inflammation of renal epithelial HK-2 cells. (
  • Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells. (
  • Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice. (
  • Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine. (
  • BACKGROUND: Arginine-vasopressin (AVP) binding to vasopressin V2 receptors promotes redistribution of the water channel aquaporin-2 (AQP2) from intracellular vesicles into the plasma membrane of renal collecting duct principal cells. (
  • These data could lead to a better understanding of aquaporin function not only at the cellular level but also in the whole plant. (
  • Wudick MM , Li X, Valentini V , Geldner N, Chory J, Lin J, Maurel C ✉, Luu D-T ✉ (2015) Sub-cellular redistribution of root aquaporins induced by hydrogen peroxide . (
  • Seventy-six cases of NSCLC were studied, including 44 cases of adenocarcinoma (ADC) and 32 cases of squamous cell carcinoma (SQCC). (
  • Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells. (
  • It is constitutively expressed in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. (
  • Immunogold EM studies demonstrated that BiP is present not only in the ER but also in the cytoplasm and apical plasma membrane of rat collecting duct cells. (
  • The water channel aquaporin-1 contributes to renin cell recruitment during chronic stimulation of renin production. (
  • Robert Stroud and colleagues, as reported in this issue of PLoS Biology, have now solved the structure of the water channel from Escherichia coli called aquaporin Z. This channel is especially interesting in that it selectively conducts only water at high rates. (
  • Aquaporin-0 functions as water channel in lens fibers. (
  • Aquaporin-Z is a major water channel in bacteria. (
  • Rai T, Sasaki S, Uchida S (2006) Polarized trafficking of the aquaporin-3 water channel is mediated by an NH2-terminal sorting signal. (
  • The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue. (
  • Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G (2003) Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but to under drought or salt stress. (
  • Therefore, an integrative approach to the relevance of the membrane-aquaporin interaction to different processes related to plant cell physiology is provided. (
  • BecauseAQP5 expression alters neutrophil cell migration, it could affect pulmonary inflammation and survival in bacterially evoked acute respiratory distress syndrome. (
  • In addition, they play a role in brain water transport, cell migration, brain edema, metabolism and cell homeostasis. (
  • Hyaluronan is required for cranial neural crest cells migration and craniofacial development. (
  • Cell viability, apoptosis, migration and invasion were detected by Cell Counting Kit‑8, flow cytometry or Transwell assay. (
  • Finally, when stimulated with cholinergic or cAMP agonists, cells from mice that lacked CFTR, as well as wild-type cells treated with a CFTR inhibitor, exhibited identical rates and magnitudes of shrinkage and Cl − efflux compared with control cells. (
  • In this study, we observed that pneumonia induced by intratracheal instillation of Pseudomonas aeruginosa (PA) in mice increased the expression of the forkhead transcription factor FoxM1 in type II cells coincidentally with the induction of alveolar epithelial barrier repair. (
  • The conjunctiva lacked goblet cells, and the anterior cortical lens was vacuolated in Klf4 CN mice. (
  • Significantly increased superficial corneal punctate lesions and decreased number of goblet cells in the conjunctiva were observed in scopolamine-injected mice. (
  • The inflammatory disorder affected normal growth and resulted in the death of the mice at ages 4-5 weeks. (
  • In addition, these mice showed an atypical distribution of aquaporin 5 in their salivary glands, suggesting likely secretory impairment. (
  • Mutations in aquaporin-0 in mice cause congenital cataracts. (
  • Electron microscopic analyses demonstrate that lungs from mice lacking CARM1 have immature alveolar type II cells and an absence of alveolar type I cells. (
  • Lgr6 + NSCLC cells displayed self-renewal and differentiation properties along with a higher tumorigenic potential. (
  • Bollag WB, Xie D, Zheng X, Zhong X (2007) A potential role for the phospholipase D2-aquaporin-3 signaling module in early keratinocyte differentiation: production of a phosphatidylglycerol signaling lipid. (
  • These basal cells migrate upward within the epidermis, initiated by the process of differentiation. (
  • Here we examined how epigenetic changes are integrated with transcriptional activation to determine cell phenotype during differentiation. (
  • We performed epigenomic profiling in conjunction with transcriptomic profiling using in vitro differentiation of human primary alveolar epithelial cells (AEC). (
  • Many studies have examined the differentiation of stem cells into a variety of differentiated cell types, in processes that traverse large phenotypic (and presumably epigenetic) distances [14] . (
  • Glucocorticoids antagonize RUNX2 during osteoblast differentiation in cultures of ST2 pluripotent mesenchymal cells J Cell Biochem. (
  • Inflammation induces irreversible biophysical changes in isolated nucleus pulposus cells. (
  • Secretin induces the apical insertion of aquaporin-1 water channels in rat cholangiocytes. (
  • Salt-induced osmotic stress also induces priming of the NLRP3 inflammasome and activates inflammatory enzymes in RPE cells. (
  • It has been suggested that Langerhans cells, a type of DCs in the cornea, can act as APCs. (
  • In this regard, a variety of stem/progenitor cells with functional specificity are responsible for both of the injury repair and the normal turnover at steady state throughout the airway tree [ 1 - 7 ]. (
  • Type II cells are described as facultative progenitor cells based on their ability to proliferate and trans-differentiate into type I cells. (
  • Role of Aquaporin 0 in lens biomechanics. (
  • Accordingly, agonist-induced serous acinar cell shrinkage and swelling are caused by activation of solute efflux and influx pathways, respectively, and cell volume reflects the secretory state of these cells. (
  • Ca 2+ signaling in salivary gland acinar cells may be interrupted by several factors, and by several intracellular pathways. (
  • Stimulation of isolated acinar cells with carbachol (CCh), histamine or ATP was associated with marked decreases in cell volume (20 ± 2% within 62 ± 5 s) that were tightly correlated with increases in cytoplasmic Ca 2+ concentration ([Ca 2+ ] i ) as revealed by simultaneous DIC and fluorescent indicator dye microscopy. (
  • Using diaminofluorescinein-2 diacetate, enhanced NO synthase activity was detected in isolated parotid acinar cells after ACh-treatment. (
  • Serous-acinar cells express the tight junction marker zona occludens-1 and measurements of transepithelial electrical resistance (TEER) demonstrate exceptional tight junction formation [routinely >2000 omega(ohms)/cm2] in a relatively short period of time (6-8 days) in Transwell inserts. (
  • Primary serous-acinar cells showed a low level of CPF metabolism to TCPy, which subsequently localized to both apical and basolateral chambers, consistent with a diffusional process and lack of TCPy concentration gradient. (
  • Effects of vascular endothelial growth factor on isolated fetal alveolar type II cells. (
  • Recent in vivo and in vitro work suggests that mesenchymal stem cells (MSC) have anti-inflammatory properties. (
  • MSC are pluripotent adult stem cells found in the bone marrow that have the capability of differentiating into multiple cell types ( 5 , 6 ). (
  • Local delivery of stem cells has been shown to be of benefit in both experimental and clinical studies of myocardial infarction ( 13 , 14 , 15 ). (
  • Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom. (
  • Our results identify a specific stem-like cell population in NSCLC with increased malignant potential, the elucidation of which may enable earlier prognosis and possibly the development of more effective targeted treatments. (
  • Recent theories in cancer research consider the existence of cell populations with stem cell-like properties in many cancer types ( 9, 10 ). (
  • Lgr6, and its homologous receptors, are amplifiers of Wnt signaling and are involved in maintaining stem cell self-renewal ( 15 ). (
  • They further proved that healthy stomach stem cells, after mutation, are a major source of stomach cancer. (
  • The high incidence rates in Asia have highlighted an unmet need to study the role of stem cells in tissue maintenance and cancer of the stomach. (
  • The study built on IMB's earlier findings of Lgr5-expressing stem cells at the gland base of the mouse stomach. (
  • The ability to isolate human stem cells for the first time also offers great potential for harnessing their regenerative medicine potential in clinical settings. (
  • Professor Nick Barker, Research Director at A*STAR IMB said, "Our work facilitates for the first time, the isolation of mouse and human stomach stem cells using antibodies, identifies dysregulation of the Wnt pathway as a frequent event in human cancer, and reveals stomach stem cells as important sources of gastric cancer. (
  • Aqp 9 and brain tumour stem cells. (
  • Developmental pathways and specification of intrapulmonary stem cells Pediatr Res. (
  • Most cases of MPAL show expression of early hemopoietic stem cell marker CD3417, MPAL without CD34 are rare. (
  • ALDH, a cancer stem cell marker enzyme, causes the AldeRed reagent to fluoresce in the far-red spectrum, allowing the cells to be identified and isolated with concurrent use of green fluorescent cell lines, transgenic animals and reporter assays. (
  • BrdU + (LRC) were the putative stem cells. (
  • BrdU + CD45 + cells increased by 0.7-fold and SPC + CC10 + bronchoalveolar stem cells (BASC), decreased by ~40-fold post-bleomycin. (
  • Most non-stem cells were non-hematopoietic in origin indicating that despite tissue turnover, BASCs are drastically depleted possibly necessitating recruitment of progenitors from the hematopoietic pool. (
  • Increase in VEGF + non-LRC may indicate a checkpoint to counterbalance the mobilization of VEGF + cells from the stem cell niche. (
  • Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. (
  • Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance. (
  • Adaptive Chromatin Remodeling Drives Glioblastoma Stem Cell Plasticity and Drug Tolerance. (
  • Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators. (
  • 1 Department of Medicine, 2 Institute for Regenerative Medicine, 3 Cardiovascular Institute, and 4 Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA. (
  • In 1999, together with other research teams, Agre reported the first high-resolution images of the three-dimensional structure of an aquaporin, namely, aquaporin-1. (
  • Aquaporin 1 forms a water-specific channel that is constitutively expressed at the PLASMA MEMBRANE of ERYTHROCYTES and KIDNEY TUBULES, PROXIMAL. (
  • Aquaporin-2 function is to reabsorb water from urine in the kidney. (
  • Polymorphisms of aquaporin-5, a key mediator of inflammation, may impact outcome.What This Article Tells Us That Is NewIn acute respiratory distress syndrome attributable to bacterial pneumonia, the C-allele of the aquaporin-5 -1364A/C promoter polymorphism is associated with less pulmonary inflammation and greater survival. (
  • Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis Am J Pathol. (
  • Aquaporin-5 is implicated in the forming of saliva, tears and pulmonary secretions. (
  • By contrast, we report here that loss of CARM1 results in hyperproliferation of pulmonary epithelial cells during embryonic development. (
  • Bellati J , Champeyroux C , Hem S , Rofidal V , Krouk G , Maurel C , Santoni V ✉ (2016) Novel aquaporin regulatory mechanisms revealed by interactomics . (
  • Aquaporin (AQP) 3 expression is altered in inflammatory bowel diseases, although the exact mechanisms regulating AQP abundance are unclear. (
  • Fundamental to understanding mechanisms of repair are identifying and characterizing the cells that are potentially capable of repopulating the injured tissue. (
  • The BBB, a specialized structure that prevents the infiltration of components from the peripheral blood into the CNS, mainly comprises brain microvascular endothelial cells (BMECs), pericytes and astrocytes ( Guillemin and Brew, 2004 ). (
  • Endocan or endothelial cell specific molecule-1 (ESM-1): a potential novel endothelial cell marker and a new target for cancer therapy. (
  • The 2003 Nobel Prize in Chemistry was awarded jointly to Peter Agre for the discovery of aquaporins and Roderick MacKinnon for his work on the structure and mechanism of potassium channels. (
  • It was not until 1992 that the first aquaporin, 'aquaporin-1' (originally known as CHIP 28), was reported by Peter Agre, of Johns Hopkins University. (
  • Agre said he discovered aquaporins "by serendipity. (
  • Agre P (2006) The aquaporin water channels. (
  • Agre went on to establish the family of related channels, which he named "aquaporins. (
  • Peter Agre discovered the first aquaporin in 1992 in red blood cells and was awarded the 2003 Nobel Prize. (
  • Cheung KH, Leung CT, Leung GP, Wong PY (2003) Synergistic effects of cystic fibrosis transmembrane conductance regulator and aquaporin-9 in the rat epididymis. (