Trypan Blue: A diazo-naphthalene sulfonate that is widely used as a stain.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Surgical Sponges: Gauze material used to absorb body fluids during surgery. Referred to as GOSSYPIBOMA if accidentally retained in the body following surgery.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Capsulorhexis: The making of a continuous circular tear in the anterior capsule during cataract surgery in order to allow expression or phacoemulsification of the nucleus of the lens. (Dorland, 28th ed)Epiretinal Membrane: A membrane on the vitreal surface of the retina resulting from the proliferation of one or more of three retinal elements: (1) fibrous astrocytes; (2) fibrocytes; and (3) retinal pigment epithelial cells. Localized epiretinal membranes may occur at the posterior pole of the eye without clinical signs or may cause marked loss of vision as a result of covering, distorting, or detaching the fovea centralis. Epiretinal membranes may cause vascular leakage and secondary retinal edema. In younger individuals some membranes appear to be developmental in origin and occur in otherwise normal eyes. The majority occur in association with retinal holes, ocular concussions, retinal inflammation, or after ocular surgery. (Newell, Ophthalmology: Principles and Concepts, 7th ed, p291)Thorium: Thorium. A radioactive element of the actinide series of metals. It has an atomic symbol Th, atomic number 90, and atomic weight 232.04. It is used as fuel in nuclear reactors to produce fissionable uranium isotopes. Because of its radioopacity, various thorium compounds are used to facilitate visualization in roentgenography.Indocyanine Green: A tricarbocyanine dye that is used diagnostically in liver function tests and to determine blood volume and cardiac output.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Evans Blue: An azo dye used in blood volume and cardiac output measurement by the dye dilution method. It is very soluble, strongly bound to plasma albumin, and disappears very slowly.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.L-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Pigment Epithelium of Eye: The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.Serum Albumin, Radio-Iodinated: Normal human serum albumin mildly iodinated with radioactive iodine (131-I) which has a half-life of 8 days, and emits beta and gamma rays. It is used as a diagnostic aid in blood volume determination. (from Merck Index, 11th ed)Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Alcian Blue: A copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.DNA Fragmentation: Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.Suramin: A polyanionic compound with an unknown mechanism of action. It is used parenterally in the treatment of African trypanosomiasis and it has been used clinically with diethylcarbamazine to kill the adult Onchocerca. (From AMA Drug Evaluations Annual, 1992, p1643) It has also been shown to have potent antineoplastic properties.Endothelium, Corneal: Single layer of large flattened cells covering the surface of the cornea.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cell Line, Tumor: A cell line derived from cultured tumor cells.Antimetabolites: Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Vitrectomy: Removal of the whole or part of the vitreous body in treating endophthalmitis, diabetic retinopathy, retinal detachment, intraocular foreign bodies, and some types of glaucoma.Nevus, Blue: Usually a benign tumor, that commonly presents as a solitary blue nodule with spindled MELANOCYTES covered by smooth SKIN. Several variants have been identified, one variant being malignant. The blue color is caused by large, densely packed melanocytes deep in the DERMIS of the nevus. In CHILDREN, they usually occur on the BUTTOCKS and LUMBOSACRAL REGION and are referred to as cellular blue nevi. Malignant blue nevi are more commonly found on the SCALP.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Tetrazolium Salts: Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455)Agglutinins: Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.Retinal Perforations: Perforations through the whole thickness of the retina including the macula as the result of inflammation, trauma, degeneration, etc. The concept includes retinal breaks, tears, dialyses, and holes.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Cell SeparationMicroscopy, Phase-Contrast: A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Hydrogen Peroxide: A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.Cytoprotection: The process by which chemical compounds provide protection to cells against harmful agents.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Tissue Preservation: The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Fluoresceins: A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.Carcinoma, Ehrlich Tumor: A transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in a mouse. It grows in both solid and ascitic forms.Immunochemistry: Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.Light: That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Cryoprotective Agents: Substances that provide protection against the harmful effects of freezing temperatures.Cornea: The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Alamar blue assay; Trypan blue assay. Primary cell lines establishment. Projects: Analysis of K2(B3O3F4OH) bioactive and ... The most frequently used tests in research projects of this lab are based on cell culture and include: chromosome aberrations ... Participation in international collaborative project: HUMNXL - Exfoliated cells micronucleus project; (2009-2011); Analysis of ... the specific chromosomal markers of basal cell carcinoma; (2007-2009) Financed by the Ministry of Education and Science of ...
Vlcková, A; Gasparic, J; Horáková, K (1971). "Investigation of the cytotoxicity of different trypan blue commercial products". ... Jantovà, S.; Horàkovà, K. (1993). "9-Hydroxybenfluron induced inhibition of proliferation and metabolism in hela cells". Cell ... "Degeneration of HeLa-cells induced by the cytotoxic effect of isothiocyanates or by the starvation of cells". Acta morphologica ... "Sacharose density gradient separation of Ehrlich ascites carcinoma and L-5178Y tumor cells in different cell cycle phases". ...
Lysis results in the cell taking up a dye (trypan blue). This allows identification of cell's MHC indirectly based on the ... An embryo can be tissue typed to ensure that the embryo implanted can be a cord-blood stem cell donor for a sick sibling. One ... In this technique a donor's blood cells are MHC typed by mixing them with serum containing the anti-HLA antibodies. If the ... antibodies recognize their epitope on the MHC then complement activation occurs and the cell will be osmotically lysed. ...
... apoptotic and normal cells. Trypan Blue, a living-cell exclusion dye Erythrosine, which is Red No. 3 in food coloring, can be ... I. CORRELATION WITH TRYPAN BLUE AND EOSIN DYE EXCLUSION AND TISSUE CULTURE TRANSFORMATION". Blood. 23 (4): 517-525. PMID ... the living cells exclude the stain i.e. stain negatively and only the dead cells stain positively and thus viability can be ... A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have ...
When a particular cell is lysed it will take up an added dye such as trypan blue allowing for identification. ... MHC Class I molecules are present on all nucleated cells and are responsible for signaling to an immune cell that an antigen ... MHC Class II molecules are only present on antigen presenting cells such as dendritic cells and are responsible for alerting ... The antibodies that bind to the cells activate a complement signaling cascade resulting in cell lysis. ...
Correlation of viability as measured by trypan blue assays were 0.97. Another application is the use of GFP co-transfection as ... GFP-labelled cancer cells have been used to model metastasis, the process by which cancer cells spread to distant organs. Macro ... In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms ... Thus, only those cells in which the tagged gene is expressed, or the target proteins are produced, will fluoresce when observed ...
During this stage the membrane becomes abnormally permeable to trypan blue and propidium iodide, indicating membrane compromise ... Apoptosis, or programmed cell death involves a series of cell shrinking processes, beginning with cell size reduction and ... "Ischemic cell death", or "Oncosis", is a form of accidental cell death. The process is characterized by an ATP depletion within ... The lack of ion transport at the cell membrane leads to an accumulation of sodium and chloride ions within the cell with a ...
... apoptotic and normal cells. Resazurin Trypan Blue, a living-cell exclusion dye (dye only crosses cell membranes of dead cells) ... "Overview of Probes for Cell Viability, Cell Proliferation and Live-Cell Function-Section 15.1". Invitrogen. Life Technologies. ... Examples: Propidium iodide Trypan blue 7-Aminoactinomycin D Mitochondrial activity or caspase assays: Resazurin and Formazan ( ... If the cells do not have high intracellular potassium and low intracellular sodium, then (1) the cell membrane may not be ...
For detection of cells general staining techniques (e.g. trypan blue) or special probes (e.g. mt-dehydrogenase detection with ... Counting of cells: positive responder cells could be counted from the front of migrating cells, after staining or in native ... Counting of cells: positive responder cells are count from the lower chamber (long incubation time) or from the filter (short ... In the original version a container drilled in a peg is filled with cells. Then the peg is twisted and the cells get contact ...
Since the cell viability is determined by electric current exclusion, viability dyes such as Trypan blue and Propidium iodide ... As the cell size is related to the cell volume, a cell size profile in cell population can be produced in terms of pulse height ... Since the cell size of each cell type is varied, before doing gating, it should ensure the correct cell size is included during ... So, for the CASY technology, as the cell flow cytometry, it can present data of each cell as a cell size distribution graph, ...
It can be measured by manual counting of cells under microscopy observation, using the dye exclusion method (i.e. trypan blue) ... where a cell, known as the "mother cell", grows and divides to produce two "daughter cells" (M phase). Cell populations go ... A cell is unable to grow to an abnormally large size because at a certain cell size or cell mass, the S phase is initiated. The ... For some cells, there is a mechanism by which cell division is not initiated until a cell has reached a certain size. If the ...
Vital stains include dyes like trypan blue and propidium iodide, which are either too bulky or too charged to cross the cell ... Supravital dyes for RBCs: New methylene blue Brilliant cresyl blue Crystal violet Methyl violet Nile blue Hoechst stain " ... By counting the number of such cells the rate of red blood cell formation can be determined, providing an insight into bone ... The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes ...
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it ... Determining the viable cell count is important for cell culture in order to calculate the dilution required to passage the ... Viable count is a method used in cell culture to determine the number of living cells in a culture. This is different from ... is taken up by dead cells and actively excluded from live cells. Once the cells have been stained they are counted using a ...
... trypan blue (a stain used to color dead tissues or cells blue), and arsenic may damage the developing fetus and cause ...
"Sigma-Aldrich, 60% Trypan Blue, Product page". Retrieved 2015-07-15. Strober, W (May 2001). "Trypan blue exclusion test of cell ... Azidine blue 3B Benzamine blue 3B Benzo Blue bB Chlorazol blue 3B Diamine blue 3B Dianil blue H3G Direct blue 14 Niagara blue ... Trypan blue is also known as diamine blue and Niagara blue. The extinction coefficient for trypan blue is 6⋅104 M−1 cm−1 at 607 ... Trypan red and trypan blue were first synthesized by the German scientist Paul Ehrlich in 1904. Trypan blue is commonly used in ...
Clemmensen I (1990). "Interaction of tetranectin with sulphated polysaccharides and trypan blue". Scand. J. Clin. Lab. Invest. ... p21.3 by somatic cell hybrid mapping". Cytogenet. Cell Genet. 76 (1-2): 39-40. doi:10.1159/000134509. PMID 9154122. Nielsen BB ... Cell. Proteomics. 3 (4): 311-26. doi:10.1074/mcp.M300127-MCP200. PMID 14718574. Gerhard DS, Wagner L, Feingold EA, et al. (2004 ... 2005). "In the search of potential human islet stem cells: is tetranectin showing us the way?". Transplant. Proc. 37 (2): 1322- ...
... including methylene blue and trypan red. This research did not bear fruit immediately, though the method was later used ... Her work on isolating the islets of Langerhans cells from the pancreas and her discovery that they secreted a substance ...
Once a T cell has recognized its particular antigen on the surface of an aberrant cell, the T cell becomes an activated ... Trypan Blue Exclusion.. Details. Pronunciation. /ˈmakrə(ʊ)feɪdʒ/. System. Immune system. Function. Phagocytosis. ... The processed antigen is then presented in MHCII on the surface of the B-cell. T cells that express the T cell receptor which ... Macrophages are a type of repairing cell that devour dead cells and pathogens, and trigger other immune cells to respond to ...
Malformations in mouse embryos induced by trypan blue. Nature 169(4288):27-28. Waddington, C. H. 1952. Selection of the Genetic ... In a period of great creativity at the end of the 1930s, he also discovered mutations that affected cell phenotypes and wrote ... Cell, 128, 635-638. Allen, Matthew. 2015. "Compelled by the Diagram: Thinking through C. H. Waddington's Epigenetic Landscape ... These points represent the eventual cell fates, that is, tissue types. Waddington coined the term chreode to represent this ...
It is then stained with a dye (trypan blue) and delineated well (Fig 2C, D). The dissected PDEK graft is then placed in a ... Adult population has a count of about 2500 to 2800 cells/Sq mm and loses around 0.6% cells per year. The cells in infant have ... Normal endothelial cell count at birth is about 4000 cells/sq mm. ... In our experience we noticed that the infant donors have maximum viable cells which can expand and cover the entire cornea in a ...
Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or ... Nile blue[edit]. Nile blue (or Nile blue A) stains nuclei blue. It may be used with living cells. ... Methylene blue[edit]. Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more ... Coomassie blue[edit]. Coomassie blue (also brilliant blue) nonspecifically stains proteins a strong blue colour. It is often ...
In nonhuman animals, diminazen (Berenil), imidocarb, or trypan blue would be the drugs of choice for treatment of B. canis ... In life-threatening cases, exchange transfusion is performed.[15] In this procedure, the infected red blood cells are removed ... Babesia parasites reproduce in red blood cells, where they can be seen as cross-shaped inclusions (four merozoites asexually ... Hemoglobinuria is seen due to excretion of red-blood-cell lysis byproducts via the kidneys. Fever of 40.5 °C (105 °F) develops ...
GAD65 and GAD67 synthesize GABA at different locations in the cell, at different developmental times, and for functionally ... Gad65 in red, Gad67 in green, and Tyrosine Hydroxylase (blue) in the Ventral Tegmental Area of the mouse brain. ... β-cells of the pancreas, in varying ratios depending upon the species.[3] Together, these two enzymes maintain the only ... Cerebellar purjinke cells also reported a 40% downregulation, suggesting that affected cerebellar nuclei may disrupt output to ...
Y. Orihara; T. Furuya (1990). "Production of theanine and other γ-glutamyl derivatives by Camellia sinensis cultured cells". ... Reactive blue 2. *Rose bengal. *SITS. *trans-ACDP. *Trypan blue. *Valinomycin. *Xanthurenic acid ... Plant Cell Reports. 9 (2): 65-68. doi:10.1007/BF00231550. PMID 24226431.. ...
Michael C. Flickinger (5 April 2010). Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology ... Reactive blue 2. *Rose bengal. *SITS. *trans-ACDP. *Trypan blue. *Valinomycin. *Xanthurenic acid ... Glutamate acts on ionotropic and metabotropic (G-protein coupled) receptors.[21] In the opposing postsynaptic cell, glutamate ... Ninety-five percent of the dietary glutamate is metabolized by intestinal cells in a first pass.[26] ...
This video demonstrates trypsinizing and cell counting using trypan blue and the Bright-Line Hemocytometer for the purpose of ... Manual Cell Counting Using Bright-Line Hemocytometer and Trypan Blue. h1 { font: bold 1.8em Arial, Helvetica, sans-serif; ... This video demonstrates the dilution of a cell solution in preparation for cell counting on the hemocytometer. Mammalian cell ... The Bright-Line Hemocytometer is the ideal lab instrument for cell counting. ...
... to ensure robust and consistent cell measurement. Manual counting with trypan blue and hemacytometers is still the most ... The need for an accurate and consistent cell counting method is crucial for the development and manufacturing of CAR-T under ... Assays are therefore required to qualify selected cell counting methods, ... Cell counting is an important step in all stages of cellular therapy. ...
Trypan blue inhibition of yolk deposition-a clue to follicle cell function in the cecropia moth ... Trypan blue inhibition of yolk deposition-a clue to follicle cell function in the cecropia moth ... Trypan blue inhibition of yolk deposition-a clue to follicle cell function in the cecropia moth ... Trypan blue inhibition of yolk deposition-a clue to follicle cell function in the cecropia moth ...
Toxicity against human Leukocyte cells assessed as reduction in cell viability by trypan blue exclusion assay. ...
Vision And Machine Learning Based Adaptable Conversıon Method For Any Light Microscope To Automated Cell Counter By Trypan Blue ... Almost all of the cell biology experiments involve counting of cells regularly to monitor cell proliferation and viability. ... Vision And Machine Learning Based Adaptable Conversıon Method For Any Light Microscope To Automated Cell Counter By Trypan Blue ... In addition, two novel data sets are collected to test our proposed method in terms of cell counting (i.e non-stained) and cell ...
Effects of Trypan Blue on Cell Viability and Gene Expression of Retinal Ganglion Cells ... Effects of Trypan Blue on Cell Viability and Gene Expression of Retinal Ganglion Cells ... Effects of Trypan Blue on Cell Viability and Gene Expression of Retinal Ganglion Cells . Invest. Ophthalmol. Vis. Sci. 2005;46( ... trypan blue. Marked reduction of Bcl-2 expression was also noted after exposure to trypan blue for 60 mins or longer (p=0.01). ...
Trypan Blue, Product page". Retrieved 2015-07-15.. *^ Strober, W (May 2001). Trypan blue exclusion test of cell viability. ... Trypan blue is also known as diamine blue and Niagara blue. The extinction coefficient for trypan blue is 6⋅104 M−1 cm−1 at 607 ... in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a ... Trypan blue is derived from toluidine, that is, any of several isomeric bases, C14H16N2, derived from toluene. Trypan blue is ...
The number of apoptotic cells was reduced in the presence of NAC. In untreated cells, endo G distributed in the cytoplasm and ... NAC prevented the increase of apoptotic cells and the translocation of endo G. Knock down of endo G diminished the cell killing ... Dual staining of cells with annexin V and propidium iodide (PI) revealed that apoptotic cell death occurred by treatment with ... cells. Upstream mediators remain largely unknown. The levels of hydrogen peroxide (H2O2) in cultured oral SCC cells were ...
The suspensions were removed and the 0.4% trypan blue were added to cells for 10 min. The trypan blue was removed and the cells ... Optical images of MCF7 cells with trypan blue-stained dead cells (middle panel) and Calcein AM stained live cells (lower panel ... we stained the cells with trypan blue and Calcein as shown Fig. 4D-G and H-K, respectively. The breast cancer cells with ... a fluorescent indicator of esterase activity in viable cells. Trypan blue assay. Cells were cultured in a 35 mm dish overnight ...
The number of blue stained cells and the total number of cells were counted. If cells took up trypan blue, they were considered ... Trypan Blue Staining. Trypan blue solution was added to the culture medium. The cells were examined immediately under an ... cell viability by MTT assay (b), mitochondrial membrane potential determined by JC-1 staining (c), cells death by Trypan Blue ... 2c). Experiments with trypan blue staining demonstrated a significant increase in number of dead cells under the eASN toxicity ...
Results: The addition of trypan blue to MMC or 5FU at concentrations up to 50% did not alter MMC induced cell death (p=0.11; ... Trypan blue is currently used to stain the anterior capsule during cataract surgery. We have recently been adding trypan blue ... The addition of trypan blue to MMC and 5FU does not affect drug cytoxicity for the assays investigated. Trypan blue can be used ... or without trypan blue at various dilutions. A lactate dehydrogenase release assay was used to measure drug-induced cell death ...
... trypan blues cytotoxic effect can further damage cells when incubation is required, thus skewing the samples viability. ... Protocols that use trypan blue to determine viability underestimate cell death. A simple argument is size - Trypan blue is a ... Identify & Resolve Manual Cell Counting Errors through Automation November 8, 2019 * Webinar: Trypan Blue vs Fluorescent Cell ... Trypan Blue: A Bull in a (Freeze-Thaw) China Shop. Scientists working on primary cells also know that TB can be cytotoxic and ...
Positive microbiology and genomic DNA typing results proved that the contaminated trypan blue solutions were the source of ... Cell viability increased after elimination of NO, and negative correlations existed between cell viability ... ... Outbreak of Multidrug-resistant Pseudomonas Aeruginosa Endophthalmitis Due to Contaminated Trypan Blue Solution.. *. ... Conclusion: Positive microbiology and genomic DNA typing results proved that the contaminated trypan blue solutions were the ...
Alamar blue assay; Trypan blue assay. Primary cell lines establishment. Projects: Analysis of K2(B3O3F4OH) bioactive and ... The most frequently used tests in research projects of this lab are based on cell culture and include: chromosome aberrations ... Participation in international collaborative project: HUMNXL - Exfoliated cells micronucleus project; (2009-2011); Analysis of ... the specific chromosomal markers of basal cell carcinoma; (2007-2009) Financed by the Ministry of Education and Science of ...
... live cell imaging and flow cytometry. This non-canonical cell death was not preceded or accompanied by exacerbation of ... live cell imaging and flow cytometry. This non-canonical cell death was not preceded or accompanied by exacerbation of ... but not in U937 cells. We further extended the anti-cancer potential of GEV to 3D cell culture using clonogenic and spheroid ... but not in U937 cells. We further extended the anti-cancer potential of GEV to 3D cell culture using clonogenic and spheroid ...
... such that IGFBP-3 then conferred cell survival. We established that IGFBP-3 activated Rho, the upstream regulator of ROCK and ... such that IGFBP-3 then conferred cell survival. We established that IGFBP-3 activated Rho, the upstream regulator of ROCK and ... We established previously that IGFBP-3 could exert positive or negative effects on cell function depending upon the ... We established previously that IGFBP-3 could exert positive or negative effects on cell function depending upon the ...
Particulate matter released from cell carrier devices during microcatheter transit measured by the number of trypan blue ... The recovered pellets were suspended in 1 mL trypan blue (0.4%) solution and vortexed. We then counted the trypan blue-stained ... Day 7: 107 cells/mL construct versus 106 cells/mL construct (P , .08); 107 cells/mL construct versus 105 cells/mL construct (P ... 20 The cell carrier devices analyzed were those that released the most debris in trypan blue assays (control and ascorbate ...
Viable cell numbers were determined through Trypan blue exclusion using the Vi-CELL Cell Viability Analyzer (Beckman Coulter). ... Cell counting using Trypan blue exclusion.. F15R-Kit cells cytokine starved for 48 h were resuspended in fresh media at a ... Cell culture. F15R-Kit cell culture.. F15R-Kit cells were a gift from the K.D. Wittrup. F15R-Kit cells were maintained at 37°C ... T cells, naive CD8+ T cells, and NK cells. IL-2 and IL-15 stimulation of T cells can result in distinct phenotypic responses ...
C, cells were incubated with various concentrations of olaparib, and after 96 h, cell viability was determined by trypan blue ... Trypan Blue Exclusion Assays. Cells were seeded in 10 mL medium and incubated overnight before treatment with inhibitor or an ... 3B) for 96 h, and viability was assessed by trypan blue exclusion. As expected, the ATM-deficient A-T cell line L3 was more ... Following the indicated incubation time, aliquots were removed and cell density and viability were determined by trypan blue ...
Cell Dyes and Detection Assay Kits (48) * Quick View. Test Trypan Blue. Reagent for counting viable mammalian cells ... The mouse prostate gland consists of three major cell types: luminal cells that secrete prostatic peptides, basal cells that ... See MoreProstate stem cells are believed to differentiate to basal transit-amplifying cells, which can then develop into ... and neuroendocrine cells that secrete peptides to support the growth and viability of luminal cells. ...
Trypan blue staining to visualize dead cells and P. infestans hyphae showed that HR cell death is induced in mesophyll cells ... Trypan Blue Staining. Trypan blue staining was done as described by Yoshioka et al. (2003), but was modified as follows. Leaves ... and 14 and was visualized by trypan blue staining (Figure 4B). Table 1 shows the percentage of leaves developing cell death by ... WRKY-induced cell death was detected by trypan blue staining (right).. (C) Detection of WRKY-HA-StrepII proteins by anti-HA ...
... cell viability determination, cryopreservation, and proliferation and apoptosis assays. ... Get tips and helpful information related to the use of our products for performing cell counting, ... Why is trypan blue solution used to determine cell viability? Trypan blue is a cell impermeant stain used to estimate the ... Remove 20 mL from the vial and dilute the cell suspension in 20 mL of trypan blue solution (for example: Gibco Trypan Blue, Cat ...
... cell viability determination, cryopreservation, and proliferation and apoptosis assays. ... Get troubleshooting help and solutions to common issues dealing with the use of our products for performing cell counting, ... How long can cells be exposed to trypan blue before all cells are stained with the dye? ... What causes trypan blue to form a precipitate? Exposure to light may degrade the dye and these contaminants may promote ...
e Cell viability was analyzed by trypan blue exclusion. Cells were treated with 50 µg/mL PM2.5 f for the indicated times or g ... Cell viability (%) was calculated as: unstained cells/(unstained cells + stained cells) × 100%. ... Trypan blue assay. Cells were seeded in 35-mm culture dishes at a concentration of 1.0 × 105 per mL, cultured for 16 h, and ... PM2.5-treated cells were stained bright blue by the ER‑Tracker Blue-White DPX, indicating the induction of ER stress which was ...
... and total cells and viable cells were counted by trypan blue exclusion as follows. Floating and adherent cells were collected ... Cell Viability: Trypan Blue Exclusion Assay. Cells were seeded in 12-well plates at a density of 5,000 per well and cultured ... trypan blue (Sigma) and scored under a light microscope. Viable (unstained) and nonviable (blue-stained) cells were counted, ... Cell Lines, Chemicals, and Viruses. We used one human epidermoid carcinoma cell line (A-431) and 10 breast cancer cell lines ...
  • Assays are therefore required to qualify selected cell counting methods, to ensure robust and consistent cell measurement. (
  • The Cellometer method has gone through rigorous qualification assays to provide rapid and accurate measurement of PBMCs, splenocytes, and apheresis samples at any stage of CAR-T cell therapy, from patient sample collection, transduction, to patient infusion. (
  • How should we prepare cell samples to perform the qualification assays? (
  • We further extended the anti-cancer potential of GEV to 3D cell culture using clonogenic and spheroid formation assays and validated our findings in vivo by zebrafish xenografts. (
  • The addition of trypan blue to MMC and 5FU does not affect drug cytoxicity for the assays investigated. (
  • Cellular therapy and processing protocols stress the importance of storage conditions and minimal temperature fluctuations to achieve suitable recovery of viable and functional cells for subsequent immune assays. (
  • Using human embryonic kidney-293 cells expressing human GLP-1Rs, we extended this work to consider the impact of compound 2 on G protein activation, Ca 2+ signaling and receptor internalization and particularly to compare compound 2 and GLP-1 across a range of functional assays in intact cells. (
  • Full assessment of the properties of compound 2 was potentially hampered by damaging effects that were particularly manifest in either longer term assays with intact cells or in acute assays with membranes. (
  • In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. (
  • What used to take us 5 people and 7 hours, now takes us only 3 people and 4 hours to process the samples, count the cells and plate them for downstream assays. (
  • Transforming growth factor (TGF)-beta, which inhibits proliferation, migration, and invasiveness of first-trimester EVT cells, also reduced invasion of isolated term EVT cells in transwell assays, whereas epidermal growth factor was a positive modulator. (
  • PGE2 and PGF2 were being dissolved in dimethlysulfoxide to ten mM and stored at 20 C. Cell viability assays Mobile viability was assessed with 3 2,5 diphenyltetrazolium bromide and trypan blue exclusion assays. (
  • C) The FB screen assays 3 distinct subsets of megakaryo/erythroid cells, YS-derived primitive RBCs, FL-derived definitive RBCs, and platelets, by 3-color FACS analysis and cell size (on logarithmic scale). (
  • Poly(ADP-ribose) polymerase-1 (PARP-1) inhibition is toxic to cells with mutations in the breast and ovarian cancer susceptibility genes BRCA1 or BRCA2 , a concept termed synthetic lethality. (
  • Significantly, Granta-519 and UPN2 cells were more sensitive to PARP-1 inhibition than were the ATM-proficient MCL cell lines examined. (
  • It follows that cells that are compromised in one DNA repair pathway may be more susceptible to inhibition of a compensatory repair pathway, leading to new opportunities for therapeutic intervention for a variety of human malignancies. (
  • Although ablation of ATM through RNA interference ( 9 ), genetic means ( 12 , 13 , 22 ), or inhibition of ATM kinase activity using a small-molecule inhibitor sensitizes cells to PARP-1 inhibitors ( 9 ), the importance of this approach for human cancers with alterations in ATM remains unknown. (
  • Wt-p53/KRAS-mutant CRC cells (HCT-116) with stem cell-like properties were treated with 100-, 150- and 200-μM/L DHA, after which cell number, viability, growth inhibition, Wt-p53, survivin and microRNA-16-1 expression, caspase-3 activation and apoptotic-rate were evaluated by different cellular and molecular techniques. (
  • Further research work with RU-486 showed a dose dependent inhibition of human melanoma cell growth. (
  • In addition, co-incubation of progesterone with RU-486 showed an additive effect on the inhibition of mouse as well as human melanoma cell growth, suggesting that the mechanism of actions of the two hormones might be different. (
  • Adherent primary cells are particularly susceptible to contact inhibition, that is, they will stop growing when they have reached confluency. (
  • Suppression of GDH activity with RNA interference or an inhibitor showed that the enzyme is dispensable in cells able to metabolize glucose but is required for cells to survive impairments of glycolysis brought about by glucose deprivation, 2-deoxyglucose, or Akt inhibition. (
  • Thus, inhibition of GDH converted these glutamine-addicted cells to glucose-addicted cells. (
  • This was primarily caused by inhibition of cell cycle progression from G 1 to S phase. (
  • ref. 6 ) in cells bearing mutations in the genes encoding DNA double-strand break (DSB) repair proteins, BRCA1 or BRCA2 ( 7 , 8 ). (
  • From these genes, each cell strain was investigated for genes that are in creased or decreased with exposure to DBP. (
  • These lists were then cross-referenced for all cell strains to determine which genes are affected by exposure in all cell strains, or only in cell strains with the same p53 haplotype. (
  • Gene expression profiles conducted on the hybrid cells confirmed the expression of genes from both of their parent cells, however, the profiles also showed that the merged cells expressed unique genes not expressed in the parent lines. (
  • The proliferative potential of some human finite cell cultures can be extended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). (
  • Among the DNMT genes, we found that mRNA for DNMT3L was specifically expressed in TGCTs, but neither in normal testicular tissues nor in cancer cells of somatic tissue origin. (
  • Genes for metalloproteinases MMP-2 and MMP9 (positive regulators of trophoblast invasiveness) were expressed up to 48 h of culturing, as also the gelatinolytic activity of the isolated cells. (
  • In addition, resveratrol has been reported to have diverse effects on cellular signaling pathways, such as downregulation of angiogenesis associated genes ( 3 ), activation of apoptotic mechanisms ( 5 ) and induction of cell cycle arrest ( 6 ). (
  • For both cell lines, RP215 and anti-antigen receptors were found to regulate similarly and consistently a number of genes including NFκB-1, IgG, P21, Cyclin D1, ribosomal P1 and c-fos with only exceptions for EGFR and ribosomal P0. (
  • Among toll-like receptor genes (TLR-2, -3, -4, -6, -7 and -9), differential levels of gene expressions in different cancer cell lines were observed. (
  • Such phenotype-driven approaches will provide new knowledge of the genes, protein interactions, and regulatory networks that underpin stem cell biology. (
  • We recommend increasing the incubation time of cells with alamarBlue™ reagent, changing the instrument's gain or voltage setting, and checking the instrument filter/wavelength settings. (
  • View popular cell culture reagents such as Dimethyl Sulfoxide (DMSO), Cell Culture reagent , PMA , and Sodium Pyruvate Solution 100 mM (100X), liquid . (
  • For this reason, there is a major emphasis on identifying proteins that can be targeted to kill cancer stem cells or control their growth, and transglutaminase type II (TGM2/TG2) is such a target in epidermal squamous cell carcinoma. (
  • In the present study, TG2 shows promise as a target for anticancer stem cell therapy in human squamous cell carcinoma. (
  • This study suggests that TG2 has an important role in maintaining cancer stem cell survival, invasive, and metastatic behavior and is an important therapeutic target to reduce survival of cancer stem cells in epidermal squamous cell carcinoma. (
  • Epidermal squamous cell carcinoma (SCC) is a common form of skin cancer that develops in response to UV light exposure ( 1 ). (
  • Epithelial tumors, including non-small cell lung cancer (NSCLC) and head and neck squamous cell carcinoma (HNSCC), present clinical challenges. (
  • Epithelial cancers, particularly lung cancer and head and neck squamous cell carcinoma (HNSCC), continue to pose formidable challenges in clinical practice. (
  • Altogether, GEV shows an interesting anticancer profile with the ability to exert cytotoxic effects via induction of different cell death modalities. (
  • IL-2 is critical for the clonal expansion of activated T cells, differentiation of effector and memory cytotoxic T lymphocytes, and regulation of T cell peripheral tolerance ( 4 , 14 , 15 ). (
  • Scientists working on primary cells also know that TB can be cytotoxic and often need to adjust their incubation time and concentration accordingly. (
  • However, after the freeze-thaw cycle, when viability drops, TB's cytotoxic effect can further damage cells when incubation is required, thus skewing the sample's viability. (
  • The cytotoxic effects of YL064 were evaluated on MM cell lines and CD138-positive primary MM cells. (
  • The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts . (
  • CpG oligonucleotides enhance the efficacy of immunization with tumor antigen in a murine B cell lymphoma model ( 2 ), induce antigen-specific cytotoxic T cell responses ( 5 , 6 ), and have utility in the immunotherapy of allergy, infectious disease, and cancer disease models ( 7 - 9 ). (
  • In this study, we investigated the effects of long-term therapy with ARA or docosahexaenoic acid (DHA) on the cytotoxic effects of the NK cells of young rats, which were fed on a nonfish oil diet for two generations. (
  • Control oil, ARA (240 mg/kg BW/day) or DHA (240 mg/kg BW/day) were orally administrated to the rats for 13 weeks before determining the cytotoxic activity of NK cells from the spleen against YAC-1 mouse lymphoma cell line, as well as the plasma levels of docosanoids or eicosanoids and inflammatory cytokines. (
  • Long-term ARA administration significantly suppressed the cytotoxic activity of NK cells. (
  • Recent studies have demonstrated that NK cells have a direct cytotoxic activity against cancer cells, and they can regulate the adaptive immune response via cytokine production [ 3 , 4 , 5 ]. (
  • Cell counting is an important step in all stages of cellular therapy. (
  • During microcatheter transit, cell carrier devices liberated minimal particulate matter and cellular DNA. (
  • Despite signaling through the same receptors, IL-2 and IL-15 have non-redundant roles in T cell biology, both physiologically and at the cellular level. (
  • The PM 2.5 -induced cell damage was attenuated by antioxidant N -acetyl cysteine, confirming that PM 2.5 cellular toxicity was due to oxidative stress. (
  • GLP-1 and compound 2 activated Gα s in cell membranes and increased cellular cAMP in intact cells, with compound 2 being a partial and almost full agonist, respectively. (
  • It has also been shown that HO-1 plays a role in cellular defense mechanism against oxidative stress induced apoptotic cell death [ 5 - 7 ]. (
  • PLD activation is now recognized as an important component of numerous cellular functions, including vesicular trafficking and cell transformation ( 19 , 20 , 21 ). (
  • The Cellaca™ MX utilizes Nexcelom's innovative cellular analysis technology for accurate cell sample concentration, viability and morphology measurements. (
  • Here we present a cellular model in which full-length human TDP-43 or a splicing isoform (TDP-S6) that lacks the C terminus is overexpressed in a human cell line and mouse primary neurons. (
  • Does HBV infection induce acute cellular D... ( Eukaryotic cells employ multiple str. (
  • Furthermore, the invention discloses a method of transfecting and/or transducing a cell, comprising engaging a macromolecule to a nanotube, placing the nanotubes into communication with the cells and applying a force to the nanotubes which causes the nanotubes to collide with the cells and thereby pierce the cellular membrane. (
  • Transcription and translation take place in mitochondria, which also actively import proteins and metabolites from the cytosol, influence programmed cell death, and respond to cellular signals such as oxidative stress ( 1 ). (
  • TNF initiates cellular inflammatory responses through engagement with two cell surface receptors: TNF receptor 1 (TNFR1) and TNFR2. (
  • If you're testing a regular cell line, make sure you have enough cells so you can make samples ranging from very high concentration to very low concentration, giving you a good dynamic range for your cell counting method. (
  • This page details a novel instrument for high-throughput, high-speed cell counting, concentration, and viability. (
  • This ago-allosteric agent, 6,7-dichloro-2-methylsulfonyl-3- N-tert -butylaminoquinoxaline (compound 2), stimulates cAMP production in membranes generated from baby hamster kidney cells expressing the human GLP-1R (hGLP-1R), albeit with a bell-shaped concentration-response curve, and also potentiates glucose-induced insulin release from pancreatic islets isolated from rodents. (
  • Trypsin may also be neutralised by the addition of soyabean trypsin inhibitor, where an equal volume of inhibitor at a concentration of 1mg/ml is added to the trypsinised cells. (
  • Measure the concentration and viability of nucleated cells in a primary SVF sample. (
  • In MCF-7 and MDA-MB231 cells, morphine caused a naloxone (Nx)- and pertussis toxin-sensitive, concentration-dependent increase of GTPase activity, indicating that morphine signals could be transduced by opioid receptors via a G protein. (
  • Right after cells had been cultured with celecoxib for three or five times, MTT was extra to a last concentration of one mgml. (
  • Angiotensin II concentration-dependently regulated basal and insulin-mediated PI3Kinase-AKT-eNOS signalling in cultured endothelial cells. (
  • Since 1cm³ is approximately equivalent to 1ml, the cell concentration per ml will be the average count per square x 104. (
  • Endo G is an endonuclease that is released from the mitochondrial intermembrane space and translocates to the cell nucleus to induce DNA fragmentation in a caspase-independent manner [ 4 - 6 ]. (
  • Freeze-thaw cycles and other manipulations stress primary cells and induce pathways of cell death. (
  • Specifically SAC was found to induce the phosphorylation of Akt and enhance the nuclear localization of Nrf-2 in cells. (
  • Extravillous trophoblast (EVT) cells are of pivotal importance in human embryo implantation and homeostasis of the maternal fetal interface. (
  • From E11.5 to E15.5, there is a massive expansion of the FL, which is initially geared for production of definitive enucleated red blood cells to sustain rapid fetal growth ( Figure 1 A). From approximately E15.5, myeloid lineages are produced and hepatocytes begin to differentiate within the FL as it transforms from a hematopoietic organ into an adult liver. (
  • 6 - 13 In animal studies, fibroblasts implanted into carotid arteries or experimental aneurysms with platinum microcoils, and vascular smooth muscle cells implanted into experimental aneurysms with collagen sponges accelerated thrombus organization and enhanced endovascular healing. (
  • 1 There are 2 waves of hematopoiesis in vertebrates ( Figure 1 A). The primitive wave is derived from hemangioblasts that form in the posterior primitive streak and migrate onto the yolk sac (YS) from embryonic day 7 (E7.0) 2 , 3 where they give rise to primitive erythroid progenitors (EryP) in association with endothelial and vascular smooth muscle cells. (
  • 2011). The released NO dissolves in the aqueous phase of the circulating blood and diffuses to reach vascular smooth muscle cells resulting in smooth muscle relaxation and vasodilation (Zhao et al. (
  • At the end of the treatment period, cells were removed for RNA isolation. (
  • We report here an improved protocol for the isolation of fully differentiated EVT cells from the basal plate of the human term placenta. (
  • This unit describes the isolation of several cell types known for their exocytotic properties, the fabrication and calibration of carbon fiber microelectrodes, as well as some of the equipment and software requirements for obtaining electrochemically generated data. (
  • Their ability to expand T cell numbers upon exogenous stimulation has made both cytokines extremely important in clinical settings as immunomodulatory and cancer immunotherapeutic agents ( 3 - 5 ). (
  • Both cytokines mediate their effects on T cells through a heterotrimeric receptor complex consisting of a cytokine specific α-chain (IL-2Rα or IL-15Rα), the common γ-chain (γ c ), and the IL-2/15R β-chain (β) ( 6 - 9 ). (
  • Of note, constitutive activation STAT3 may play a more crucial role in the pathogenesis of MM. In the bone marrow environment, cytokines such as IL-6, secreted by stromal cells or the myeloma cells, could lead to the constitutive activation of STAT3. (
  • FM significantly inhibited the activation of NF-κB and STAT3 signaling in HCC cells induced by cytokines (TNF-α or IL-6) and in co-culture system with CD8 + NKG2D + cells. (
  • Compared with GMCSF, CpG-treated peripheral blood DCs showed enhanced functional activity in the mixed lymphocyte reaction and induced T cells to secrete increased levels of Th1 cytokines. (
  • Following this, the researchers exposed the hybrid cells to a variety of cell-signalling molecules known as cytokines which help to modulate the immune response. (
  • This behavior of trypan blue was first described in the scorpian fly by Ramamurty (1964), and has since been confirmed in a cricket (Sander & Vollmar, 1967) and in the cecropia moth (Telfer & Anderson, 1968). (
  • the hybrid cells might be taking advantage of the greater growth potential of cancer cells and the greater mobility of macrophages.Silk et al plan on next investigating the behavior of these hybrid cells in the mouse's circulatory system. (
  • Different cell types vary greatly with respect to their growth behavior and nutritional requirements. (
  • Less is known about the behavior of large I/D heterologies in mammalian cells. (
  • These ECS cells are able to generate tumors in immune-compromised mice following subcutaneous injection of as few as 100 cells ( 9 ). (
  • Using the Eμ-myc model of B cell lymphoma, we screened tumors with defined genetic alterations in apoptotic pathways for therapeutic responsiveness to the HDACi vorinostat. (
  • In a final experiment, Silk's team injected the hybrid cells into mice - finding that tumor growth was more rapid than tumors grown from non-fused cells. (
  • Hematopoietic cells (derived from blood, spleen, or bone marrow) as well as some transformed cell lines and cells derived from malignant tumors can be grown in suspension. (
  • However, cell cultures derived from human tumors are often immortal. (
  • Testicular germ cell tumors (TGCT) have a unique epigenetic profile distinct from that of other types of cancer. (
  • Accurate diagnosis of EC is critical for the management of patients with stage I nonseminomatous testicular germ cell tumors because the percentage of EC in the primary tumor is predictive of occult metastasis. (
  • Testicular germ cell tumors (TGCT) are the most common malignancy in young men, with a peak incidence between 20 and 40 years of age. (
  • and administration of therapeutic agents modulating the susceptibility fgjhd, IFN-g, GM-CSF, TNF-a, MIP-1a and MIP-1b compared of tumors to NK-cell lysis has been proposed recently. (
  • Bcl-2 and its homologue Bcl-XL are expressed in a variety of tumors and their expression modulates the sensitivity of tumor cells to a wide spectrum of chemotherapeutic agents and γ-irradiation. (
  • The ataxia telangiectasia mutated ( ATM ) gene is altered in several human cancers including mantle cell lymphoma (MCL). (
  • Human proximal tubular cells (HK-2) were incubated (5 to 60 min) with human albumin and IgG (1 to 30 mg/ml). (
  • Experimental and human data in recent years have suggested that proteins filtered through the glomerular capillary barrier in excessive amount have an intrinsic renal toxicity linked to their over-reabsorption by proximal tubular cells and activation of tubular-dependent pathways of interstitial inflammation ( 1 , 2 ). (
  • CD8 + NKG2D + cells were derived from human peripheral blood and were used for evaluation of immune cells-mediated inflammation and oncolysis on HCCLM3 cells. (
  • 6. The method of claim 1 , wherein said β-cells are human β-cells. (
  • Methods are disclosed for inhibiting the infectivity of HIV-1 in human cells. (
  • contacting HIV-1 infected human cells with an effective HIV-1 replication inhibiting amount of chloroquine or a pharmaceutically acceptable salt thereof. (
  • The present invention is concerned with the use of certain quinolinyl and acridinyl derivatives to inhibit the infectivity of Human Immunodeficiency Virus-1 in human cells. (
  • The present invention is concerned with methods of inhibiting the infectivity of Human Immunodeficiency Virus-1 (HIV-1) in human cells infected therewith. (
  • DNA molecules containing unmethylated CpG-dinucleotides in particular base contexts ("CpG motifs") are excellent adjuvants in rodents, but their effects on human cells have been less clear. (
  • Three different protocols, each using a different cell of origin, are in use to obtain human DCs. (
  • We previously performed a small molecule screen using Fluidigm high throughput qPCR platform to identify compounds that alter globin gene expression in human erythroid cells to identify compounds that produce favourable effects for β-thalassaemia. (
  • Hence, the study was extended to check the effect of RU-486 alone on human melanoma (BLM) cell growth. (
  • Results showed that RU-486 inhibited human melanoma (BLM) cell growth significantly in a dose dependent manner. (
  • In contrast, human primary cell cultures rarely, if ever, become immortal in this way and require additional genetic manipulation to form a continuous cell line. (
  • and wherein said perfusate comprises nucleated placental cells from said human placenta. (
  • Serotonin receptor expression, signal transduction, and 5-HT effects on breast cancer cell phenotype were compared in non-transformed and transformed human breast cells. (
  • Correspondingly, the tightly regulated pattern of 5-HT receptors becomes dysregulated in human breast cancer cells, resulting in both ectopic expression of some isoforms and suppression of others. (
  • In our prior investigations of chemokine-induced signal transduction, we had demonstrated the potent induction of phospholipase D (PLD) activity by the C-X-C chemokine IL-8 in human T lymphocytes and T cell clones ( 18 ). (
  • MCF-7 [wild-type p53, estrogen receptor positive (20) ] and MDA-MB231 [p53 mutation (21) , estrogen receptor negative (22) ] human breast cancer cells were cultured in DMEM and RPMI 1640, respectively, supplemented with 10% FCS and 1% penicillin/streptomycin at 37°C in a 5% CO 2 atmosphere. (
  • Fast analysis for cell count and viability, using a very small amount of sample, makes Cellaca™ MX suitable for droplet-based single-cell transcriptome analysis, utilized to analyze primary samples from human and animal models. (
  • Sigilon Therapeutics encases novel human cells in a proprietary immune-shielding matrix. (
  • Isolated cells were immunoreactive for cytokeratin-7 (CK-7), placental growth factor, placental alkaline phosphatase, human leukocyte antigen G1 (HLA-G1), and α1 and α5 integrins, similarly to the EVT markers from first trimester placental villi. (
  • 18) chromosomal translocation found in human B-cell lymphomas. (
  • mda- 7) was enhanced by OSU-03012 in a diverse panel of primary human GBM cells. (
  • Collectively, our data demonstrate that the induction of autophagy and mitochondrial dysfunction by a combinatorial treatment approach represents a potentially viable strategy to kill primary human GBM cells. (
  • mda- 7, inhibits the growth and kills a broad spectrum of cancer cells, without exerting any harmful effects in normal human epithelial or fibroblast cells. (
  • 5 . The method of claim 1 wherein the cell is a human B cell. (
  • Human Cell 14:244‐247. (
  • In a recent SelectScience ® webinar , Dr. Leo Chan of Nexcelom Biosciences discusses the advantages of Cellometer automated cell counters over traditional trypan blue and manual counting methods. (
  • Development and characterization of cell strains was achieved using standard methods.17 Cells were grown in MEGM media (Clonetics, Cambrex, Pittsburgh, PA) at 37°C and 5% CO2 . (
  • Primary cell cultures come from the outgrowth of migrating cells from a piece of tissue or from tissue that is disaggregated by enzymatic, chemical, or mechanical methods. (
  • Methods and Results- Primary cultures of adult rat ventricular myocytes (ARVMs) were subjected to preconditioning (PC) by exposing the cells to 10 minutes of hypoxia followed by 30 minutes of reoxygenation. (
  • The Cellaca MX revolutionizes how scientists count and characterize cells, providing high accuracy and extremely fast speeds of 2 - 17 seconds per sample using either Trypan Blue or Fluorescent detection methods. (
  • PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. (
  • Santa Cruz Biotechnology now offers a broad range of Cell Culture Reagents. (
  • Cells no longer sit on ice waiting to be counted, making the data output for our proteomic, genomic, and flow cytometry applications more accurate. (
  • Depending on their origin, animal cells grow either as an adherent monolayer or in suspension. (
  • 8. The isolated adherent placental cell population of claim 7 , wherein said perfusate consists of perfusate that has been collected from placental vasculature. (
  • After 1, 30, 60, 120, 360 mins the cultures were processed to assess the effects of trypan blue on cell viability and gene expression. (
  • In addition, stimulation of T cells with IL-2 or IL-15 has been shown to result in the induction of similar gene expression profiles ( 8 , 12 , 13 ). (
  • Integrin alpha4 (alpha4) mediates stromal adhesion of normal and malignant B-cell precursors, and according to gene expression analyses from 207 children with minimal residual disease, is highly associated with poorest outcome. (
  • 1999 ) reported a similar low rate of intrachromosomal gene conversion of a 2.4-kb insertion in mouse cells. (
  • The degree of adhesion varies from cell line to cell line but in the majority of cases proteases, e.g. trypsin, are used to release the cells from the fl ask. (
  • This is important as cell adhesion is related to a cancer cell's mobility, which in turn is related to its metastatic potential. (
  • Integrin alpha4 expression inversely correlates with clinical outcomes of pre-B ALL patients and mediates adhesion-dependent chemoprotection in leukemia cells. (
  • One manifestation of these signals is the secretion of IL-2 and the up-regulation of numerous cell surface adhesion receptors ( 15 , 17 ). (
  • Subsequently, it was further documented that RP215 not only reacts with the epitope of immunoglobulin heavy chains, but also many other immunoglobulin superfamily (IgSF) proteins including antigen receptors, such as immunoglobulins and T-cell receptors, as well as cell adhesion molecules [ 3 , 4 ]. (
  • Glutathione is the most abundant nonprotein sulfhydryl compound and the major intracellular redox buffer in almost all cells. (
  • Western blotting For western blot analyses, cells have been washed with PBS and lysed in lysis buffer as explained previously. (
  • Signal transduction initiated by IGFBP-3 in T47D breast cancer cells, was shown to require TGF-beta receptors and its signaling intermediates, Smad-2 and -3. (
  • Despite signaling through the same receptors and sharing common effector pathways, multiple studies have reported unique and even antagonistic roles for IL-2 and IL-15 in the T cell immune response. (
  • Dendritic cells (DCs) form the link between the innate and the acquired immune system by presenting antigens and by their expression of pattern recognition receptors that detect microbial molecules in their local environment. (
  • Src regulates signals from multiple cell surface molecules, including integrins, growth factors, and G protein-coupled receptors. (
  • RP215 is a monoclonal antibody generated against a carbohydrate-associated epitope of glycoproteins designated as CA215, which consists mainly of immunoglobulin superfamily proteins expressed by cancer cells including antigen receptors such as immunoglobulins and T-cell receptors. (
  • RP215 and anti-antigen receptors were found to up-regulate TLR-2 and/or TLR-3, whereas those of TLR-4 and TLR-9 were down regulated for both cancer cells. (
  • The innate immunity of cancer cells can also be affected by any of these antibodies through unidirectional regulations of certain toll-like receptors. (
  • PlGF preferentially forms heterodimers with VEGF that are mitogenic toward endothelial cells capable of binding with high affinity to both tyrosine kinase receptors VEGF receptors VEGFR-1 and -2 ( 8 ). (
  • SS: Does AO bind to nucleotides that could be present in the media after the lysing cells/long cultures? (
  • Immunostaining and RT-PCR showed a significant increase in Bax expression in cultures treated with either 0.6 mg/ml (p = 0.032) or 4 mg/ml (p = 0.025) trypan blue. (
  • The purpose of this study is to explore the cytoprotective effects of the antioxidant Bucida buceras extract in co-treatment with hydrogen peroxide (H 2 O 2 ) delivery as a single addition or with continuous generation using glucose oxidase (GOx) in ARPE-19 cell cultures. (
  • This is especially necessary for serum-free cell cultures. (
  • Primary cells, finite cultures, and continuous cell lines differ in their proliferative potential (see below). (
  • These cultures are capable of only a limited number of cell divisions, after which they enter a non-proliferative state called senescence and eventually die out. (
  • Primary cell cultures are sometimes preferred over continuous cell lines in experimental systems. (
  • These cultures will proliferate for a limited number of cell divisions, after which they will senesce. (
  • Use of such cells is sometimes easier than use of primary cell cultures, especially for generation of stably transfected clones. (
  • Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. (
  • Elimination of mycoplasma from cell cultures and establishment of mycoplasma‐free cell lines. (
  • The need for an accurate and consistent cell counting method is crucial for the development and manufacturing of CAR-T under GMP/GLP conditions. (
  • Manual counting with trypan blue and hemacytometers is still the most prevalent method, despite several inherent issues with the technique that can cause inaccuracy in both cell count and viability. (
  • This thesis proposes state-of-the-art alternative method (i.e. framework) for the cell counting by defining computer vision and machine learning based conversion methodology. (
  • In addition, two novel data sets are collected to test our proposed method in terms of cell counting (i.e non-stained) and cell viability analysis (i.e. stained). (
  • The experimental results also validate that the proposed method can be a powerful alternative to the current cell counting approaches. (
  • Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. (
  • ATLANTA , April 1, 2019 /CNW/ -- Nexcelom Bioscience, a leading provider of cell counting and characterization products for the life science industry, announced the launch of the revolutionary high throughput, high-speed automated cell counter - Cellaca™ MX. (
  • According to Nexcelom CTO, Dr. Jean Qiu , the company is focused on enabling highly accurate cell counting at fast speeds in order to help accelerate other technology advancements. (
  • The choice between manual and automated cell counting has long been a difficult one. (
  • Manual cell counting is accurate but often time-consuming and subject to user error. (
  • Automated cell counting is faster and less user-dependent, but disposable slide consumables can be expensive. (
  • The new Corning® Cell Counter is the first cell counter that combines the best features of automated and manual cell counting. (
  • The Corning Cell Counter uses cloud-based image processing to perform a cell count in less than three seconds*, allowing it to analyze images faster than any existing automated cell-counting system. (
  • The Corning Cell Counter uses a reusable glass counting chamber, enabling automated cell counting without the cost of disposable slides. (
  • As the name suggests it has been developed for counting blood cells but it is now widely established in a huge variety of fields. (
  • Very important using this technique is to avoid errors like: unequal cell distribution in the sample, improper filling of chambers, failure to adopt a convention for counting cells in contact with the boundaries lines or with each other. (
  • Microscopic cell counting is also useful as a preliminary step for making colony counts. (
  • Besides that, counting cells of spoilage organisms during aging is like a effective insurance. (
  • Mammary Epithelial Growth Medium Bulletkit is a readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells. (
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (
  • Primary cells are morphologically similar to the parent tissue. (
  • DNMT3L protein was strongly expressed in two EC cell lines, but not in the cell lines of somatic tissue origin. (
  • Immunohistochemical analysis showed that DNMT3L protein was present only in EC cells, but not in the other types of TGCT components and cancer cells of somatic tissue origin. (
  • administering to a subject in need of such treatment a rho GTPase function inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject, provided that the rho GTPase function inhibitor is not an agent selected from the group consisting of a HMG-CoA reductase inhibitor, a protein kinase C inhibitor, a tyrosine kinase inhibitor, and cyclosporin. (
  • 12. The method of claims 1 - 9 , further comprising co-administering at least one different rho GTPase function inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject. (
  • Mitochondrial proteins are attractive targets for the study of metabolism of the chondrocyte, the unique cell type present in mature cartilage, and its role in tissue degradation. (
  • The only cell type present in mature cartilage is the chondrocyte, which is responsible for repairing cartilage tissue damaged by OA. (
  • administering to a nonhypercholesterolemic subject in need of such treatment a HMG-CoA reductase inhibitor in an amount effective to increase endothelial cell Nitric Oxide Synthase activity in said tissue of the subject. (
  • Dendritic cells (DCs) form the link between the innate and the acquired immune system and may influence the balance between T helper 1 (Th1) and Th2 immune responses. (
  • Here, we present a protocol to develop and characterize tolerogenic dendritic cells (TolDCs) and evaluate their immunotherapeutic utility. (
  • Plasmacytoid dendritic cells (pDCs) are specialized type I interferon (IFN-α/β)-producing cells that express intracellular toll-like receptor (TLR) 7 and TLR9 and recognize viral nucleic acids in the context of infections. (
  • This article discusses how an over-estimation of cell viability due to trypan blue rupturing dead or dying immune cells can be reduced. (
  • A recent study to disprove the idea that some cancer and immune cells can merge and form a new, more potent cancer cell, actually ended up proving the validity of the theory - scattered evidence for which has been accumulating for quite some time. (
  • Further, macrophages, being immune cells, are ready-made to fuse with other cells, and tumor cells typically contain a great many macrophages (and cancer cells naturally fuse together). (
  • So, the idea that cancer cells and immune cells are capable of fusing would not seem so surprising. (
  • For example, the company combines a personalized anticancer vaccine protocol, which causes the release of tumor-specific markers, and the injection of bioengineered allogeneic immune cells into the lesion as an adjuvant to modulate the immune response and educate the immune system to kill other tumor cells. (
  • Tumor necrosis factor (TNF), a proinflammatory cytokine secreted mostly by immune cells, plays an important role in the pathophysiology of sepsis. (
  • TNFR1 is found on immune cells, such as macrophages ( 8 ), and parenchymal cells, such as hepatocytes ( 9 ). (
  • p27, an inhibitor of CDK2, was not translocated into the nucleus in erlotinib-resistant cell lines. (
  • In the case of cells cultured in serum-free media, use a trypsin inhibitor e.g. soyabean trypsin inhibitor to inactivate the trypsin. (
  • This protective effect was inhibited by treating the cells with LY294002 (50 μmol/L), a PI3 kinase inhibitor, for 10 minutes before and during PC. (
  • 14. The method of claims 1 - 9 , further comprising co-administering a non-rho GTPase function inhibitor agent that increases endothelial cell Nitric Oxide Synthase activity. (
  • In 1 analyze of mice implanted with the Sunitinib c-Kit hepatoma cell line H22, cure with the COX 2 inhibitor nimesulide led to reductions in both COX 2 selleckchem Pazopanib and Bcl 2 expression. (
  • Analysis of the mechanism(s) of 22 Na + influx was performed in control and acid-activated (addition of 10 mmoll -1 proprionic acid) cells using a variety of Na + transport inhibitors (ouabain, phenamil, HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). (
  • DUCORD, HPC (Hematopoietic Progenitor Cell), Cord Blood, is an allogeneic cord blood hematopoietic progenitor cell therapy indicated for use in unrelated donor hematopoietic progenitor cell transplantation procedures in conjunction with an appropriate preparative regimen for hematopoietic and immunologic reconstitution in patients with disorders affecting the hematopoietic system that are inherited, acquired, or result from myeloablative treatment. (
  • Unit selection and administration of DUCORD should be done under the direction of a physician experienced in hematopoietic progenitor cell transplantation. (
  • A) Schematic of hematopoietic development focusing on the circulating blood cell types and FL progenitor cell types assayed in our E14.5 screen. (
  • We elected to screen the blood and FL at E14.5 so as to gain access to primitive and definitive blood cells (∼ 50:50 mix), and all definitive progenitor cell types. (
  • Used as basal medium for supporting the growth of many different mammalian cells. (
  • Vitellogenic oocytes can also sequester the acidic colloid trypan blue: when injected into the blood, the dye, like the blood proteins, is deposited in yolk spheres in the cortex of the oocyte. (
  • Promising applications include embolic materials modified with biologically active proteins and endoaneurysmal implantation of living cells. (
  • In rats with renal mass reduction, albumin and IgG accumulation by proximal tubular cells preceded the interstitial infiltration of inflammatory cells, which concentrated almost exclusively at the sites of proximal tubules congested with proteins ( 4 ). (
  • When placed in the body, these cells, Shielded Living Therapeutics, produce therapeutic proteins in a programmable and durable fashion, without generating fibrosis or immune rejection. (
  • Thus, these kinases relay and amplify the damage signal and effector proteins that control the cell cycle progression, chromatin restructuring and DNA repair. (
  • In the present studies, we transfected FL5.12 lymphoid cells with expression constructs that produce Flag-epitope tagged Bcl-2 and Bcl-XL and developed cell clones that express similar levels of these proteins as determined with a Flag-specific antibody. (