Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Cellular Phone: Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.Speech: Communication through a system of conventional vocal symbols.Foundations: Organizations established by endowments with provision for future maintenance.Friends: Persons whom one knows, likes, and trusts.Politics: Activities concerned with governmental policies, functions, etc.Police: Agents of the law charged with the responsibility of maintaining and enforcing law and order among the citizenry.Text Messaging: Communication between CELL PHONE users via the Short Message Service protocol which allows the interchange of short written messages.Developing Countries: Countries in the process of change with economic growth, that is, an increase in production, per capita consumption, and income. The process of economic growth involves better utilization of natural and human resources, which results in a change in the social, political, and economic structures.Skates (Fish): The common name for all members of the Rajidae family. Skates and rays are members of the same order (Rajiformes). Skates have weak electric organs.Reminder Systems: Systems used to prompt or aid the memory. The systems can be computerized reminders, color coding, telephone calls, or devices such as letters and postcards.Elasmobranchii: A subclass of cartilaginous fish comprising the SHARKS; rays; skates (SKATES (FISH);), and sawfish. Elasmobranchs are typically predaceous, relying more on smell (the olfactory capsules are relatively large) than sight (the eyes are relatively small) for obtaining their food.Privacy: The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)Genetic Privacy: The protection of genetic information about an individual, family, or population group, from unauthorized disclosure.Confidentiality: The privacy of information and its protection against unauthorized disclosure.Computer Security: Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.Crime: A violation of the criminal law, i.e., a breach of the conduct code specifically sanctioned by the state, which through its administrative agencies prosecutes offenders and imposes and administers punishments. The concept includes unacceptable actions whether prosecuted or going unpunished.Supreme Court Decisions: Decisions made by the United States Supreme Court.Superstitions: A belief or practice which lacks adequate basis for proof; an embodiment of fear of the unknown, magic, and ignorance.Nanotubes, Peptide: NANOTUBES formed from cyclic peptides (PEPTIDES, CYCLIC). Alternating D and L linkages create planar rings that self assemble by stacking into nanotubes. They can form pores through CELL MEMBRANE causing damage to cells.Physician Executives: Physicians who serve in a medical and administrative capacity as head of an organized medical staff and who also may serve as liaison for the medical staff with the administration and governing board.Science: The study of natural phenomena by observation, measurement, and experimentation.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Education, Graduate: Studies beyond the bachelor's degree at an institution having graduate programs for the purpose of preparing for entrance into a specific field, and obtaining a higher degree.Oscillatoria: A genus of filamentous CYANOBACTERIA in the order Oscillatoriales. It is commonly found in freshwater environments, especially hot springs.MichiganCancer Care Facilities: Institutions specializing in the care of cancer patients.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Physics: The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Comprehensive Health Care: Providing for the full range of personal health services for diagnosis, treatment, follow-up and rehabilitation of patients.Gentian Violet: A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Rosaniline Dyes: Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Contrast Media: Substances used to allow enhanced visualization of tissues.Gadolinium: Gadolinium. An element of the rare earth family of metals. It has the atomic symbol Gd, atomic number 64, and atomic weight 157.25. Its oxide is used in the control rods of some nuclear reactors.Gadolinium DTPA: A complex of gadolinium with a chelating agent, diethylenetriamine penta-acetic acid (DTPA see PENTETIC ACID), that is given to enhance the image in cranial and spinal MRIs. (From Martindale, The Extra Pharmacopoeia, 30th ed, p706)Atractylodes: A plant genus of the family ASTERACEAE. Members contain hinesol and atractylon. Atractylodes rhizome is Byaku-jutsu. A. lancea rhizome is So-jutsu.Apiaceae: A large plant family in the order Apiales, also known as Umbelliferae. Most are aromatic herbs with alternate, feather-divided leaves that are sheathed at the base. The flowers often form a conspicuous flat-topped umbel. Each small individual flower is usually bisexual, with five sepals, five petals, and an enlarged disk at the base of the style. The fruits are ridged and are composed of two parts that split open at maturity.Radiology: A specialty concerned with the use of x-ray and other forms of radiant energy in the diagnosis and treatment of disease.Phagocytes: Cells that can carry out the process of PHAGOCYTOSIS.Dermatitis, Allergic Contact: A contact dermatitis due to allergic sensitization to various substances. These substances subsequently produce inflammatory reactions in the skin of those who have acquired hypersensitivity to them as a result of prior exposure.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Rheumatic Diseases: Disorders of connective tissue, especially the joints and related structures, characterized by inflammation, degeneration, or metabolic derangement.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Rheumatic Fever: A febrile disease occurring as a delayed sequela of infections with STREPTOCOCCUS PYOGENES. It is characterized by multiple focal inflammatory lesions of the connective tissue structures, such as the heart, blood vessels, and joints (POLYARTHRITIS) and brain, and by the presence of ASCHOFF BODIES in the myocardium and skin.Rheumatic Heart Disease: Cardiac manifestation of systemic rheumatological conditions, such as RHEUMATIC FEVER. Rheumatic heart disease can involve any part the heart, most often the HEART VALVES and the ENDOCARDIUM.

Migration of intravenously grafted mesenchymal stem cells to injured heart in rats. (1/306)

The present study aimed to determine the role of tissue injury in migration of mesenchymal stem cells (MSCs) intravenously transplanted into heart and to establish experimental basis for improving stem cell therapy in its targeting and effectiveness. MSCs were isolated from bone marrow of male Sprague-Dawley rats and purified by density centrifuge and adhered to the culture plate in vitro. Female rats were divided randomly into four groups. Myocardial ischemia (MI) transplanted group received MSCs infusion through tail vein 3 h after MI and compared with sham-operated group or normal group with MSCs infusion, or control group received culture medium infusion. MI was created in female rats by ligating the left anterior descending coronary artery. The heart was harvested 1 week and 8 weeks after transplantation. The characteristics of migration of MSCs to heart were detected with expression of sry gene of Y chromosome by using fluorescence in situ hybridization (FISH). Ultrastructural changes of the ischemic myocardium of the recipient rats were observed by transmission electron microscope (TEM). One week or 8 weeks after transplantation, sry positive cells were observed in the cardiac tissue in both of MI transplanted group and sham-operated group, the number of sry positive cells being significantly higher in MI transplanted group (P<0.01). No significant difference was found in the number of sry positive cells between 1 week and 8 weeks after transplantation. No sry positive cells were observed in the hearts of control and normal group. In addition, the ultrastructure of some cells located in the peri-infarct area of MI rats with MSCs transplantation was similar to that of MSCs cultured in vitro. These results indicate that MSCs are capable of migrating towards ischemic myocardium in vivo and the fastigium of migration might appear around 1 week after MI. The tissue injury and its degree play an important role in the migration of MSCs.  (+info)

Environmental enrichment requires adult neurogenesis to facilitate the recovery from psychosocial stress. (2/306)


Non-invasive cell tracking in cancer and cancer therapy. (3/306)

Cell-based therapy holds great promise for cancer treatment. The ability to non-invasively track the delivery of various therapeutic cells (e.g. T cells and stem cells) to the tumor site, and/or subsequent differentiation/proliferation of these cells, would allow better understanding of the mechanisms of cancer development and intervention. This brief review will summarize the various methods for non-invasive cell tracking in cancer and cancer therapy. In general, there are two approaches for cell tracking: direct (cells are labeled with certain tags that can be detected directly with suitable imaging equipment) and indirect cell labeling (which typically uses reporter genes approach). The techniques for tracking various cell types (e.g. immune cells, stem cells, and cancer cells) in cancer are described, which include fluorescence, bioluminescence, positron emission tomography (PET), single-photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI). Non-invasive tracking of immune and stem cells were primarily intended for (potential) cancer therapy applications while tracking of cancer cells could further our understanding of cancer development and tumor metastasis. Safety is a major concern for future clinical applications and the ideal imaging modality for tracking therapeutic cells in cancer patients requires the imaging tags to be non-toxic, biocompatible, and highly specific. Each imaging modality has its advantages and disadvantages and they are more complementary than competitive. MRI, radionuclide-based imaging techniques, and reporter gene-based approaches will each have their own niches towards the same ultimate goal: personalized medicine for cancer patients.  (+info)

In vivo molecular imaging using nanomaterials: general in vivo characteristics of nano-sized reagents and applications for cancer diagnosis. (4/306)


Non-invasive imaging of human embryonic stem cells. (5/306)

Human embryonic stem cells (hESCs) hold tremendous therapeutic potential in a variety of diseases. Over the last decade, non-invasive imaging techniques have proven to be of great value in tracking transplanted hESCs. This review article will briefly summarize the various techniques used for non-invasive imaging of hESCs, which include magnetic resonance imaging (MRI), bioluminescence imaging (BLI), fluorescence, single-photon emission computed tomography (SPECT), positron emission tomography (PET), and multimodality approaches. Although the focus of this review article is primarily on hESCs, the labeling/tracking strategies described here can be readily applied to other (stem) cell types as well. Non-invasive imaging can provide convenient means to monitor hESC survival, proliferation, function, as well as overgrowth (such as teratoma formation), which could not be readily investigated previously. The requirement for hESC tracking techniques depends on the clinical scenario and each imaging technique will have its own niche in preclinical/clinical research. Continued evolvement of non-invasive imaging techniques will undoubtedly contribute to significant advances in understanding stem cell biology and mechanisms of action.  (+info)

Non-surgical stem cell delivery strategies and in vivo cell tracking to injured myocardium. (6/306)


Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging. (7/306)


Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. (8/306)


*Mobile phone tracking

"Cell Tracking". Electronic Frontier Foundation. n.d. "U.S. Spy Program Targeted Cellphones Of U.S. Citizens". 14 ... regarding government tracking of individuals. In In re Application of the United States for Historical Cell Site Data, 724 F.3d ... "Tracking a suspect by any mobile phone: Tracking SIM and handset". BBC News. 2005-08-03. Retrieved 2010-01-02. "Location Based ... In order to route calls to a phone, the cell towers listen for a signal sent from the phone and negotiate which tower is best ...


Cell Sense White Paper Archived July 8, 2011, at the Wayback Machine. Fluorine‐containing nanoemulsions for MRI cell tracking V ... "Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking." Cytotherapy ... Labeled cells are transplanted into the patient enabling researchers and clinicians to non-invasively track the administration ... Applications include tracking cells in immunotherapy or regenerative medicine as well as diagnosis of inflammatory sites by ...

*Somatic embryogenesis

Time-lapse tracking in Norway Spruce Picea abies revealed that neither single cytoplasmic-rich cells nor vacuolated cells ... Description of somatic-embryo- forming single cells in carrot suspension cultures employing video cell tracking. Planta 194: ... synchronous cell-division and time-lapse tracking. The latter typically works more consistently because of cell-cycle-altering ... Time-lapse tracking by Toonen et al. (1994) showed that morphology of competent cells can vary based on shape and cytoplasm ...

*Fate mapping

"Cell tracking using a photoconvertible fluorescent protein." Nature Protocols 1 (2006): 960-7. Kuhn, Ralph, Frieder Schwenk, ... to follow movements of individual cells or groups of cells over time in Xenopus frog embryos. The tissue(s) to which the cells ... At a given stage of development, one can use a UV laser to uncage the compound in a distinct cell or set of cells, effectively ... When carried out at single-cell resolution, this process is termed cell lineage tracing. The first attempts at fate mapping ...

*Papa Roach discography

Dead Cell (track listing). Papa Roach. DreamWorks Records. 2001. "Modern Rock - Available for Airplay Archive". FMQB. Mediaspan ... Before I Die (track listing). Papa Roach. Eleven Seven Music. 2013. Love, Bret. "Ozzfest 2001: The Second Millennium - Various ...

*Graphene quantum dot

Multifunctional Graphene Quantum Dots for Combined Photothermal and Photodynamic Therapy Coupled with Cancer Cell Tracking ... OPV solar cells, and photoluminescent material, biosensors fabrication. Ritter, Kyle A.; Lyding, Joseph W. (15 February 2009 ...

*Some Bizzare Records

It included the Soft Cell track "God Shaped Hole", recorded by the newly reformed band. The compilation also saw the inclusion ... After Soft Cell signed to Some Bizzare, he went on to manage Soft Cell, under a deal with Phonogram Inc. Their cover of " ... After working with B-Movie the label achieved notable success with Soft Cell, an electronic duo whose Mutant Moments EP Stevo ... Soft Cell, The The, Neu Electrikk and Blancmange. One of the first bands that Some Bizzare worked with was B-Movie. ...

*Kaede (protein)

In spite of the usefulness in cell tracking and cell visualization of Kaede, there are some limitations. Although Kaede will ... The cell-cell borders of daughter cells are visualized and the position and distance between two or more cells can be described ... The photoconversion property of Kaede does not only contribute to the application on protein labeling and cell tracking, it is ... By Kaede protein, a particular pair of daughter cells in neighboring Kaede-positive cells in the ventricular zone of mouse ...

*Alvin J. Siteman Cancer Center

"Nanoparticles used to track cells". UPI. 27 July 2007. Retrieved 25 July 2012. Melcer, Rachel (27 July 2007). "Innovating with ... Highlights and ongoing studies include these projects: 2017 - CAR-T cell therapy and using Zika virus to fight brain cancer In ... High-tech goggles developed by Samuel Achilefu, Ph.D., help surgeons see cancer cells in real time. The technology, which ... Louis and four other medical centers receive transplants of their healthy bone marrow cells to determine how effective the ...

*In re Application of the United States for Historical Cell Site Data

It argued that cell site data information can track people while they are inside their homes, which the Supreme Court has held ... Fakhoury, Hanni (July 31, 2013). "New Court Ruling Makes it Easier for Police to Track Your Cell Phone". Electronic Frontier ... 2010). Soghoian, Chris (1 Oct 2012). "Tuesday: Federal Appeals Court Hears Important Cell Phone Tracking Case". Retrieved 10 ... It did not rule on other forms of mobile location tracking, like GPS tracking or the collection of data from all phones using a ...


... tracking of individual cells over time, detection of class-transition motifs (e.g. cells entering mitosis), and HMM correction ... computer vision and machine learning techniques for single-cell tracking and classification of cell morphologies. This enables ... "Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells". ... CellCognition has been used in RNAi-based screening, applied in basic cell cycle study, and extended to unsupervised modeling. ...

*Tornado outbreak of April 3, 2012

Once the cells tracked eastward towards Louisiana, the atmosphere was much more stable and the cells rapidly weakened. The city ... During the morning of April 3, a large low-pressure area and associated frontal boundaries tracked across the Southern US ... That development was attributed to an outflow boundary from another area of storms farther north in Oklahoma that tracked ... A low pressure system and associated frontal boundaries tracked across the Southern Plains on April 3. The large-scale ...

*Live single-cell imaging

Live cell tracking is generally split into two steps, image segmentation of cells or their nuclei and cell/nuclei tracking ... Following live cell imaging, automated tracking software is then employed to extract time series data from videos of cells. ... In the final stage of a live single-cell imaging study modelling and analysis of time series data extracted from tracked cells ... Live cell imaging of fluorescently labelled cells must then be performed. This requires simultaneous incubation of cells in ...

*Tornado outbreak of May 18-21, 2013

The most intense cell tracked from Dallas County to Story County, producing three brief tornadoes and straight-line winds along ... Around 2:30 p.m. CDT (1930 UTC), a supercell developed over Harper County, Kansas and tracked northeastward. This cell produced ... At 4:41 p.m. CDT (2141 UTC), a large EF3 tornado developed 3 mi (4.8 km) northwest of Luther and tracked for roughly 20 mi (32 ... Tracking northeast, the system rapidly intensified, attaining EF4 intensity within seconds of touching down. After crossing the ...

*United States v. Davis (2014)

This information can be used to track a cell phone user's movements throughout the day. Quartavius Davis, on trial with five co ... "Supreme Court Passes on Cell Phone Tracking Case". Roll Call. Retrieved 2015-11-10. David Bryan Sentelle, C.J. (11 June 2014 ... As a cell phone caller moves, their connection is automatically handed-off to another cell site that is close by, as needed. ... a cell phone service provider. Mobile phone tracking data had helped place the defendant in this case at the scene of several ...

*Eshel Ben-Jacob

75 1226-1229 (1995). ^ "Swarming and complex pattern formation in Paenibacillus vortex studied by imagine and tracking cells". ... The decision-circuits of the individual bacteria are coupled by exchange of chemical messages between the cells to guarantee ... and inter-cell calcium dynamics,[62][63][64] using nano technology for network engineering,1 discovery and modeling of the ... that is adjusted by the cell stress) via a special decision-circuit composed of a cascade of inhibitions. ...

*Eos (protein)

EosFP has been used to track cell movements during embryonic development of Xenopus laevis. At the two-cell/ early gastrula ... These variants have been successful in the tracking of cellular components without disturbing function in the host cell and ... These fusion proteins allow for the tracking of proteins within living cells while retaining complex biological functions like ... mEos fusion proteins allow for differential colour labelling in single cells, or groups of cells in developing organs. They can ...

*Olfactory ensheathing glia

"Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and ... be rejected by the body and biological functions such as cell adhesion and growth will be enhanced through cell-cell and cell- ... "CD46 on glial cells can function as a receptor for viral glycoprotein-mediated cell-cell fusion". Glia. 52 (3): 252-8. doi: ... By labeling OEG, these cells can be tracked by a magnetic resonance imaging (MRI) device when being dispersed in the central ...

*Tornado outbreak of March 2-3, 2012

During the afternoon, those cells tracked eastward across the Ohio Valley, passing north of Louisville, Kentucky and south of ... Along its track, the tornado destroyed hundreds of homes and killed 11 people. The most severe damage took place in and around ... This destructive, long-track nighttime tornado first touched down in a sparsely populated area of Wolfe County, Kentucky at 6: ... One long-track EF4 tornado ripped through the Indiana towns of New Pekin, Henryville, Marysville, and Chelsea, killing 11 ...


Ashby MC, Ibaraki K, Henley JM (2004) It's green outside: tracking cell surface proteins with pH-sensitive GFP. TRENDS in ... It has also been applied to the study of insulin secretion in beta cells of the pancreas. ...

*Law of Gravity (CSI: Crime Scene Investigation)

They begin to track his cell phone. Brass calls Trenton PD and learns about the history between Frank and Keppler. He calls ... Archie and Greg track Keppler to a deserted field in Henderson using his car's GPS, but Keppler disables it. ... Archie finds a voice mail from Suzy, the blonde, on Courtney's cell phone. In it, Suzy tells her friend Frank kept calling her ... Nick and Warrick head out to the deserted field in Henderson, where they find a cooler with the hand and Keppler's cell phone ...

*Mount Hood climbing accidents

Initial information and cell phone tracking indicated he was northwest of Zig Zag Canyon. Five searchers were unable to find ... At 9 am, cell phone contact was established and rescuers learned they spent the night below the tree line. Rescuers escorted ... On Sunday, December 10, 2006 James made a cell phone call to his wife, and two older sons telling them that he was trapped in a ... Thinking they had a clear weather window, they had no GPS, nor Mountain Locator Unit, and did not believe their cell phone was ...

*Semen analysis

Most systems are based on image analysis, but alternative methods exist such as tracking cell movement on a digitizing tablet. ... The sample may also be tested for white blood cells. A high level of white blood cells in semen is called leucospermia and may ... A man can have a total number of sperm far over the limit of 20 million sperm cells per milliliter, but still have bad quality ... The other way around, a man can have a sperm count far less than 20 million sperm cells per millilitre and still have good ...

*Tornadoes of 2011

Tornadoes were scattered that day until early evening, when an intense tornadic cell tracked near the Little Rock metropolitan ... A destructive tornado tracked across the region with severe damage in several communities including houses destroyed in ... A total of 50 homes sustained varying degrees of damage along the tornado's track. The most severe damage took place at a local ... As a line of powerful cells began to take shape, trained spotters reported large tornadoes near El Reno, Oklahoma and in rural ...

*Paenibacillus vortex

Ingham CJ, Ben-Jacob E: Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells. ... Cell 2006, 125:237-246. Bischofs IB, Hug JA, Liu AW, Wolf DM, Arkin AP: Complexity in bacterial cell-cell communication: quorum ... Paenibacillus vortex possesses advanced social motility employing cell-cell attractive and repulsive chemotactic signaling and ... Cell 2008, 135:1153-1156. Sirota-Madi A, Olender T, Helman Y, Ingham C, Brainis I, Roth D, Hagi E, Brodsky L, Leshkowitz D, ...


Photoelectric cells in a spectrophotometer device worn on the forehead measure the amount of each wavelength of light reflected ... Patient progress will be tracked using the same measure at the beginning and end of every neurotherapy session. Single photon ...
C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with latrunculin A. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video2 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.. ...
The purposeful, synchronised travel paths of the hundreds of thousands of bacteria are captured in a brilliantly colourful time-lapse image
C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.. ...
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In recent years, nanotechnology has emerged as one of the most promising fields for biomedical applications and healthcare. The use of nanoparticles has earned much attention due to their small size, high surface area to volume ratio along with surface charge, and their optical and semiconducting properties. Superparamagnetic iron oxide nanoparticles (SPIONs), which show superparmagnetism, have been reported to be reliable and safe for many biological applications.In the present study, 10nm SPION were loaded with anticancer drug, Docetaxel. Docetaxel conjugation to SPION was confirmed by FTIR, UV-vis spectroscopy and gel electrophoresis. SPIONs uptake by cells was investigated using Prussian blue dye staining.In vitro drug release was studied which showed 55% drug release in 24 h. Drug loaded SPION were then tested on Mammary tumor cells (4T1) and showed 40% cell viability with 0.1M Docetaxel conjugated to SPION. Our results shows that Docetaxel-SPIONs can reduce the amount of required anticancer drug
Page contains details about c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles :
We demonstrate that fatty acid modified iron oxide prolonged retention of the contrast agent in the polymer matrix during degradative release of drug. Antibody-fatty acid surface modification facilitated cellular targeting and subsequent internalization in cells while inducing clustering of encapsulated fatty-acid modified superparamagnetic iron oxide during particle formulation. This induced clustered confinement led to an aggregation within the nanoparticle and, hence, higher transverse relaxivity, r2, (294 mM−1 s−1) compared with nanoparticles without fatty-acid ligands (160 mM−1 s−1) and higher than commercially available superparamagnetic iron oxide nanoparticles (89 mM−1 s−1). ...
Adipose-derived stem cells (ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide (SPIO) nanoparticles are critical for magnetic resonance (MR) tracking of implanted cells. Hypoxia is a powerful stimulus for angiogenic activity of ASCs. In this study, we investigated whether therapeutic potency could be enhanced by implantation of hypoxia-preconditioned SPIO-labeled ASCs (SPIOASCs) into the infarcted myocardium. ASCs and SPIOASCs were cultured under 2% O2 (hypoxia) or 95% air (normoxia). Cells were intramyocardially injected into the infarcted myocardium after 48-h culture. We found that hypoxia culture increased the mRNA expression of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in ASCs and SPIOASCs. The VEGF protein in the conditioned medium was significantly higher in hypoxic ASCs and SPIOASCs than in normoxic ASCs and SPIOASCs. The capillary density and left ventricular contractile function in the
The density of proteins in solution is an important fundamental biophysical quantity that has not been accurately measured yet. This has led to extensive debate in the literature regarding the precise density value of proteins. This rough density metric can often lead to inaccurate estimates on the other physicochemical and composite protein features, especially after their interactions with other materials such as nanoparticles. Researchers have now demonstrated a new technique using superparamagnetic iron oxide nanoparticles for magnetic levitation, which promises a more precisely measurement of the density of proteins in solution.
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Entering service in 1848, the Needle Gun was used extensively by Prussian forces during the Austro-Prussian and Franco-Prussian Wars.
Tracking vaccine components from the site of injection to their destination in lymphatic tissue, and simultaneously monitoring immune effects, sheds light on the influence of vaccine components on particle and immune cell trafficking and therapeutic efficacy. In this study, we create a hybrid particle vaccine platform comprised of porous silicon (pSi) and superparamagnetic iron oxide nanoparticles (SPIONs). The impact of nanoparticle size and mode of presentation on magnetic resonance contrast enhancement are examined. SPION-enhanced relaxivity increased as the core diameter of the nanoparticle increased, while encapsulation of SPIONs within a pSi matrix had only minor effects on T2 and no significant effect on T2* relaxation. Following intravenous injection of single and hybrid particles, there was an increase in negative contrast in the spleen, with changes in contrast being slightly greater for free compared to silicon encapsulated SPIONs. Incubation of bone marrow-derived dendritic cells (BMDC) with
Application of hyperthermia induced by superparamagnetic iron oxide nanoparticles in glioma treatment André C Silva1, Tiago R Oliveira1,2, Javier B Mamani1, Suzana MF Malheiros3,4, Luciana Malavolta1, Lorena F Pavon1, Tatiana T Sibov1, Edson Amaro Jr1,5, Alberto Tannús6, Edson LG Vidoto6, Mateus J Martins6, Ricardo S Santos6, Lionel F Gamarra11Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil; 2Instituto de FÃ-sica, Universidade de São Paulo, São Paulo, Brazil; 3Departament of Neurology and Neurosurgery, Universidade Federal de São Paulo, São Paulo, Brazil; 4Neuro-Oncology Program of Hospital Israelita Albert Einstein, São Paulo, Brazil; 5Instituto de Radiologia, Faculdade de Medicina; 6CIERMag-Instituto de FÃ-sica de São Carlos, Universidade de São Paulo, São Paulo, BrazilAbstract: Gliomas are a group of heterogeneous primary central nervous system (CNS) tumors arising from the glial cells. Malignant gliomas account for a majority of
In the past few years, the use of nanostructured materials in medical applications hasdramatically increased, both in the research phase and for clinical purposes, due to thepeculiar properties and the ability of such materials to interact at a similar scale withbiological entities. In this thesis, we developed tailored magnetic multifunctionalnanoparticles for diagnostic and therapeutic applications, such as detection ofbiomolecules, simultaneous enhanced magnetic resonance imaging (MRI), fluorescentvisualization and controlled drug release.For sensitive and selective detection of specific biomolecules, thermally blocked ironoxide nanoparticles with tailored magnetic properties were developed. The formation ofsuch nanoparticles has been studied both in terms of size and magnetic behavior in liquidsuspension or in polymer matrixes. These particles with narrow size distribution (averagediameter of 19 nm) were surface functionalized by antigen molecules and were used forthe detection of Brucella ...
Acute Oral Toxicity: In different studies, Prussian blue insoluble (14038-43-8) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments data in rodents, i.e. most commonly in rats for Prussian blue insoluble (14038-43-8). The different studies on target substance are summarized as below - In another experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance Prussian blue insoluble (14038-43-8). Acute oral toxicity study was done in rats using Prussian blue (14038-43-8).No mortality was observed at dose 8000mg/kg bw.Hence LD50 was considered to be ,8000 mg/kg body weight,when rats were treated with Prussian blue (14038-43-8)orally. Also these results are further supported by the experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance ...
Gene therapy shows promise for individualized cancer therapies. Compared to conventional treatments, this approach offers a variety of advantages, including a multiplicity of gene targets that define tumor clinical behavior, high specificity, low off target toxicity, and suppression of drug resistance. However, the lack of efficient delivery strategies for gene-specific agents and preclinical models for evaluation of the efficacy of gene-based therapeutics has impeded its clinical application. Nanoparticles provide a flexible platform for fabricating novel delivery vehicles capable of transporting gene-specific therapeutic agents to target tumors. Among the various types of nano-carriers for cancer therapy, superparamagnetic iron oxide nanoparticles have emerged as a leading candidate as they have a host of advantages compared to other formulations. The iron oxide core facilitates the condensation of other molecules to it that confer biological properties that promote stability during transport ...
Time-Lapse Reflection Imaging of Matrix Assembly - Collagen, type I, or pepsin-solubilized intestinal submucosal, PSIS, was polymerized in 8-well chambered coverglass (Lab-Tek, Nalge Nunc Int.). The unstained 3-dimensional matrices were viewed with an inverted confocal laser-scanning microscope (BioRad MRC1024) using a 60X, oil immersion lens. Laser light of 488 nm illuminated the hydrated samples and the reflected light was detected with a photomultiplier tube using a blue reflection filter. Samples were maintained at 30-37°C on a heated stage. Time-lapse images of fibrillogenesis were recorded at 10-15 second intervals. For standardizing the scanning depth in the matrix, the Z position was kept in the range of 20 to 50 µm from the coverglass.. All material (text, images and movies) copyright Purdue University. No material may be reproduced in any form without the express permission of Purdue University. ...
The Prussian blue (cyanide-bridged, ordered double perovskite) analogue potassium imidazolium hexacyanoferrate, (C3N2H5)2K[Fe(CN)6], contains imidazolium cations encapsulated within a metal-cyanide framework. These are free to rotate in the intermediate- and high-temperature phases, but freeze into fixed orientatio
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Studying the effect of particle size and coating type on the blood kinetics of superparamagnetic iron oxide nanoparticles Farnoosh Roohi, Jessica Lohrke, Andreas Ide, Gunnar Schütz, Katrin DasslerMR and CT Contrast Media Research, Bayer Pharma AG, Berlin, GermanyPurpose: Magnetic resonance imaging (MRI), one of the most powerful imaging techniques available, usually requires the use of an on-demand designed contrast agent to fully exploit its potential. The blood kinetics of the contrast agent represent an important factor that needs to be considered depending on the objective of the medical examination. For particulate contrast agents, such as superparamagnetic iron oxide nanoparticles (SPIOs), the key parameters are particle size and characteristics of the coating material. In this study we analyzed the effect of these two properties independently and systematically on the magnetic behavior and blood half-life of SPIOs.Methods: Eleven different SPIOs were synthesized for this study. In the first set
Limited research has suggested iron oxide nanoparticles (FeNP) have an inhibitory effect against several different genera of bacteria: Staphylococcus, Bacillus and Pseudomonas spp. In this study we looked at the effect of three different sets of Fe3O4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. Two of the tested NPs were SPIONs (Superparamagnetic Iron Oxide Nanoparticles). Exposure of cells to the SPIONs at concentrations up to 200 μg/ml resulted in an increase in biofilm biomass by 16 h under static conditions and a corresponding increase in cell density in the bulk liquid. In contrast, these biofilms had decreased levels of extracellular DNA (eDNA). Fe(II) levels in the supernatants of biofilms formed in the presence of FeNPs exceeded 100 μM compared with 20 μM in control media without cells. Spent cell supernatants had little effect on Fe(II) levels. Cells also had an effect on the aggregation behavior of these nanoparticles. SPIONs incubated with cells
Completely water dispersible and highly monodispersed superparamagnetic iron oxide nanoparticles (SPIONs) were prepared. The surface of SPIONs was modified with dual-crosslinked amine activated dextran (AMD) and chemical cleavage of AMD on SPIONs was carried out by ethylenediamine hydrochloride (EDA). Transmission electron microscopy (TEM) revealed that individual SPIONs were completely separated in water and the average diameter of resulting nanoparticles was 4.4 nm.. ...
Spinal cord injury (SCI) is a condition that results in significant mortality and morbidity. Treatment of SCI utilizing stem cell transplantation represents a promising therapy. However, current conventional treatments are limited by inefficient delivery strategies of cells into the injured tissue. In this study, we designed a magnetic system and used it to accumulate stem cells labelled with superparamagnetic iron oxide nanoparticles (SPION) at a specific site of a SCI lesion. The loading of stem cells with engineered SPIONs that guarantees sufficient attractive magnetic forces was achieved. Celý text » ...
Sigma-Aldrich offers abstracts and full-text articles by [Bin Zhou, Dan Li, Jiesheng Qian, Zhengran Li, Pengfei Pang, Hong Shan].
Article Immunosensor Based on Gold Nanoparticles-multi-walled Carbon Nanotubes-chitosans Composite and Prussian Blue for Detection of Chlorpyrifos, in Sensors and Transducers Journal, Vol. 146, Issue 11, November 2012, pp.121-132
Invented by Sir John Herschel in 1841, this simple process gives a continuous-tone image of Prussian Blue using a sensitising.... ...
Pada kesempatan ini, Binmas mengajak warga agar bersama sama di wilayah untuk mengantisipasi curat, curas dan curanmor dan congkel spion, serta selalu mewaspadai dan mencegah terjadinya curanmor curas dan curat dilingkungan kawasan pemukiman warga optimalkan pam swakarsa.. Kemudian Binmas mengimbau agar memantau dan mengawasi segala bentuk guantibmas terhadap lingkungan di sekitarnya. Apabila terjadi guantibmas agar segera menghubungi pihak Kepolisian terdekat atau polsubsektor terdekat dan tidak main hakim sendiri apabila ada pelaku tindak pidana tertangkap tangan. Tujuan patroli dialogis ini untuk mencegah bertemunya niat dan kesempatan di lokasi-lokasi rawan guantibmas serta menggali informasi yang ada di masyarakat sebagai masukan dalam melaksanakan tugas polri untuk terciptanya wilayah yang kondusif.. ( ...
Å hekle på glidelås er så lettvindt. Denne lille mappa kan bli det meste. Teknikken er veldig grei å kunne. To crochet direct ...
Å hekle på glidelås er så lettvindt. Denne lille mappa kan bli det meste. Teknikken er veldig grei å kunne. To crochet direct ...
Adferdsproblemer og andre fysiske eller psykiske problemer kan være knyttet til proteinintoleranse(matintoleranse). Npif arbeider for at kunnskapen om dette skal bli kjent og anerkjent.
Adferdsproblemer og andre fysiske eller psykiske problemer kan være knyttet til proteinintoleranse(matintoleranse). Npif arbeider for at kunnskapen om dette skal bli kjent og anerkjent.
The magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) have made them useful tools in a variety of applications ranging from drug delivery to magnetorheologic valving in microfluidic systems. The structure of SPIONs is typically characterized by methods such as XRD, XAFS, SEM and high resolution TEM while the SPION magnetic properties are evaluated relative to bulk values using SQUID. These methods characterize the state of the nanoparticles; however, the methods provide limited information about the behavior of the particles in solution. Here we demonstrate that electron paramagnetic resonance (EPR) reveals the solution behavior of 13 nm SPIONs suspended in a methanol:glycerol solution as well as environmental changes around the SPIONs. For example, characteristic temperature-dependent changes in the EPR spectra detail orientation changes of the SPIONs. The data presents a new opportunity for observing the in vitro, in vivo, and solution behavior of magnetite SPIONs.
A comprehensive literatures update of clinical researches of superparamagnetic resonance iron oxide nanoparticles for magnetic resonance imaging
Abstract Background In order to increase understanding of how infused cells work, it becomes important to track their initial movement, localization, and engraftment efficiency following transplantation. However, the available in vivo cell tracking techniques are suboptimal. The study objective was to determine the biodistribution of intravenously administered Indium-111 (In-111) oxine labeled human umbilical tissue-derived cells (hUTC) in a rat model of transient middle cerebral occlusion (tMCAo) using single photon emission computed tomography (SPECT). Methods Rats received 3 million In-111 labeled hUTC (i.v.) 48 hrs after tMCAo. Following the administration of either hUTC or equivalent dose of In-111-oxine (18.5 MBq), animals underwent SPECT imaging on days 0, 1, and 3. Radioactivity in various organs as well as in the stroke area and contralateral hemisphere was determined, decay corrected and normalized to the total (whole body including head) radioactivity on day 0. Immunohistochemical analysis
Figure 1. Time-lapse images of spindles assembled in Xenopus egg extract around a bead coated with RCC1, the guanine nucleotide exchange factor for Ran (green, upper panel) or a bead coated with chromatin (blue, lower panel). This experiment shows that a single protein (RCC1) can induce spindle assembly, but the bead oscillates within the spindle.. Size Control. Size varies widely in biology at many levels: the animal, the cells that make up the animal, and the contents of the cells, but we dont understand how scaling occurs so that everything fits and functions properly. Correct organelle size is crucial for cell function, architecture, and division, but the control systems that a cell uses to regulate the size of its organelles are virtually unknown. Macromolecular cellular assemblies like the ribosome or nuclear pore self assemble from a fixed number of subunits and do not vary in size. In contrast, many complex organelles adjust in size to suit a particular cellular environment. Loss of ...
Downloadable! This paper provides a documentation of the ifo Prussian Economic History Database (iPEHD), a county-level database covering a rich collection of variables for 19th-century Prussia. The Royal Prussian Statistical Office collected these data in several censuses over the years 1816-1901, with much county-level information surviving in archives. These data provide a unique source for micro-regional empirical research in economic history, enabling analyses of the importance of such factors as education, religion, fertility, and many others for Prussian economic development in the 19th century. The service of iPEHD is to provide the data in a digitized and structured way.
Crystal Structures of Photo-induced Phase and Rapidly-cooled Phase in Rb_,0.73,Mn[Fe(CN)_6]_,0.91,・1.4H_2O Prussian Blue Analog (2006 ...
Nalotimagene carmaleucel, an ex vivo cell therapy for immune reconstitution in patients with high-risk acute leukaemia who are undergoing haplo-identical
In this study, magnetic iron oxide nanoparticles (Fe3O4) with the size range of 20-30 nm were prepared by the modified controlled chemical co-precipitation method from the solution of ferrous/ferric mixed salt-solution in alkaline medium. In this process polyethylene glycol was used as a surfactant to prevent the solution from agglomeration. The prepared magnetic nanoparticles were characterized by X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM) and vibrating-sample magnetometer (VSM). XRD image indicates the sole existence of inverse cubic spinel phase of magnetic iron oxide nanoparticles (Fe3O4). SEM image show that the dimension of magnetic iron oxide nanoparticles (Fe3O4) is about 24 nm. VSM patterns demonstrate superparamagnetic properties of magnetic nanoparticles.
Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEONLA-BSA, which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEONLA-BSA particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro
The in vitro labelling of cultured cells with iron oxide nanoparticles (NPs) is a frequent practice in biomedical research. To date, the potential cytotoxicity of these particles remains an issue of debate. In the present study, 4 different NP types
Page contains details about polyvinylpyrrolidone-capped core-shell mesoporous gadolinium-containing Prussian blue nanocrystals . It has composition images, properties, Characterization methods, synthesis, applications and reference articles :
3) was probably synthesized for the first time by the paint maker Diesbach in Berlin around the year 1706.[18] Most historical sources do not mention a first name of Diesbach. Only Berger refers to him as Johann Jacob Diesbach.[19] It was named Preußisch blau and Berlinisch Blau in 1709 by its first trader.[20] The pigment replaced the expensive lapis lazuli and was an important topic in the letters exchanged between Johann Leonhard Frisch and the president of the Royal Academy of Sciences, Gottfried Wilhelm Leibniz, between 1708 and 1716.[20] It is first mentioned in a letter written by Frisch to Leibniz, from March 31, 1708. Not later than 1708, Frisch began to promote and sell the pigment across Europe. By August 1709, the pigment had been termed Preussisch blau; by November 1709, the German name Berlinisch Blau had been used for the first time by Frisch. Frisch himself is the author of the first known publication of Prussian blue in the paper Notitia Coerulei Berolinensis nuper inventi in ...
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How to coat 96 well plate with poly-L-Lysine for cell culture use? - posted in -Immunology and Neuroscience-: How to coat 96 well plate with Poly-L-lysine?
  • We present a combined report on the results of three editions of the Cell Tracking Challenge, an ongoing initiative aimed at promoting the development and objective evaluation of cell tracking algorithms. (
  • A novel framework for objective evaluation of cell tracking algorithms has been established under the auspices of the IEEE International Symposium on Biomedical Imaging 2013 Cell Tracking Challenge. (
  • Arbab AS, Pandit SD, Anderson SA et al (2006) MRI and confocal microscopy studies of magnetically labeled endothelial progenitor cells trafficking to sites of tumor angiogenesis. (
  • Fricker RA, Carpenter MK, Winkler C, Greco C, Gates M., Bj鰎klund A. (1999) Site - specific migration and neural differen-tiation of human neural progenitor cells after transplantation into the adult rat brain. (
  • The exceptional cellular uptake and intracellular boron release, together with the fluorescent and biocompatibility properties, make compounds 7 and 8 good candidates for in vitro cell tracking. (
  • Nevertheless, feasibility of clinical translation has been achieved by showing the localization of USPIO-labeled dendritic cells in cervical lymph nodes of patients by clinical MRI. (
  • Ahrens ET, Feili-Hariri M, Xu H et al (2003) Receptor-mediated endocytosis of iron-oxide particles provides efficient labeling of dendritic cells for in vivo MR imaging. (
  • doi:10.1002/mrm.22702 "Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking. (
  • Detection of the (19) F signal offers high cell specificity and quantification ability in spin density-weighted MR images. (
  • In this paper we introduce an optical method for simultaneous visualization and cell quantification, which combines the laser scanning confocal microscopy (LSCM) and the in vivo flow cytometry (IVFC). (
  • c ) Quantification of hypointense spots within the phantoms shows a significant increase in hypointense spots with increasing number of embedded labelled cells. (
  • The Voxel Tracker software program enables visualization and quantification of MRI-labeled cells. (
  • Computational tools enable cell or inflammation quantification in regions of interest. (
  • Researchers at Rice and the Texas Heart Institute at St. Luke's Episcopal Hospital in Houston reported in the journal Biomaterials that mesenchymal stem cells drawn from pig bone marrow labeled with GNTs are easily spotted under MRI. (
  • After injecting bone marrow stem cells labeled with these particles into the hearts of healthy mice, the researchers found that they could track the cells using a standard ultrasound device. (
  • Washington, D.C.-The Electronic Frontier Foundation (EFF) urged the U.S. Supreme Court to review a troubling ruling that allows police to obtain-without a warrant-location data from people's cell phones to track them in real time. (
  • Jersey and Delaware must show probable cause before tracking people's cell phone locations. (
  • Its mazelike design allows a group of cells to sort themselves into a starting lineup then migrate across a set of tight parallel tunnels whose narrowness mimics that of the lymphatic vessels that metastatic cells have to travel through. (
  • Metastatic breast cancer characterized by EDEMA and ERYTHEMA of the affected breast due to LYMPHATIC METASTASIS and eventual obstruction of LYMPHATIC VESSELS by the cancer cells. (
  • In our experience, a preliminary titration at constant cell concentration is the easiest way to select an optimized dye concentration that gives acceptable post-labeling viability, recovery, and fluorescence intensity as well as unaltered cell function 2,3 . (
  • Deutsch M, Kaufman M, Shapiro H, Zurgil N. (2000) Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements Cytometry 39, 36 - 44. (
  • Cell-tracking approaches using optical methods are important for preclinical research. (
  • We discuss the compositions of matter, methods and applications of PFC-based cell tracking using ex vivo and in situ PFC labeling in preclinical studies of inflammation and cellular therapeutics. (
  • HeLa cells were labeled with Alexa Fluor 488 Click-iT Plus EdU to indicate new DNA synthesis. (
  • Moreover, the cytotoxicity and cellular uptake of compounds 5 - 9 have been analyzed in vitro at different concentrations of B (5, 50, and 100 μg B/mL) using HeLa cells. (
  • Noninvasive imaging of fewer or even single cells succeeds with MRI after labeling of the cells with (ultrasmall) superparamagentic iron oxide particles (SPIO and USPIO). (
  • Repetitive MRI acquisition enabled dynamic observation of iron oxide nanoparticle (ION) labelled cells. (
  • To answer this several methods exist to track cells, such as transfection of cells with LacZ carrying viruses, radio labeling with 111 IN or 51 Cr or fluorescent labeling with FDA. (
  • Cell Sense: is a fluorocarbon tracer agent used to safely and efficiently label cells ex vivo without the use of transfection agents. (
  • The eight distinct probe colors are resistant to photobleaching and provide intense punctate fluorescence within the cell, with different emissions from a single excitation for easy combination with antibody staining. (
  • They discovered that the cells showed two distinct patterns of division. (
  • These results point to a distinct subset of cells acting as the engine of tumor growth. (
  • However, in order to gain an acceptable signal-to-noise ratio, at a sufficiently high spatial resolution of the MR sequence to visualize a small amount of cells, experimental MR scanners working at high magnetic fields are usually required. (
  • Anderson SA, Shukaliak-Quandt J, Jordan EK et al (2004) Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis. (
  • The study has determined GNTs are effective in helping doctors track stems cells through the body by making them 40 times better than standard contrast agents used in magnetic resonance imaging. (
  • Tests show GNTs are highly effective for tagging and tracking stem cells through magnetic resonance imaging. (
  • Celsense makes proprietary imaging agents for cell trafficking using magnetic resonance imaging (MRI) techniques. (
  • The labeling protocol is rapid, simple, and applicable to essentially any membrane-containing cell or particle ( Figure S2 *), but obtaining bright, uniform, and reproducible labeling requires attention to different variables than when staining with antibodies or other equilibrium binding reagents 1-3 . (
  • Nanoparticle imaging also demonstrated remarkable sensitivity, as the researchers were able to visualize as few as 75,000 stem cells with ultrasound, and a few hundred thousand with MRI. (
  • In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. (
  • The simplest processes for monitoring cell movement and location use tracking probes that pass through the membrane into the cell and become membrane-impermeant after loading. (
  • U2-OS cells were transduced with CellLight Plasma Membrane-GFP (green), followed by labeling with Qtracker 655 Cell Labeling Kit (magenta), and HCS NuclearMask Blue (blue). (
  • The amount of dye required for bright, homogeneous labeling increases as total number and/or size of cells to be stained increases (i.e., as total amount of membrane increases). (
  • Table S1 * summarizes non-perturbing staining conditions reported for several membrane dyes, but concentrations found in the literature should always be tested on each laboratory's cell type(s) of interest. (
  • Diluent C is an aqueous staining vehicle designed to keep cells viable and membrane dyes in solution for long enough to complete the brief labeling step. (
  • It minimizes membrane dye aggregation and maximizes staining efficiency by avoiding salts, while minimizing cell toxicity by maintaining osmotic pressure and avoiding organic solvents. (
  • Since labeling occurs by partitioning rather than equilibrium binding, dye uptake into membrane continues for as long as cells are in contact with dye, eventually compromising membrane integrity and function. (
  • Changes in membrane potential, oxidative stress and intracellular calcium were simultaneously assessed for every single cell during these time points. (
  • 1989) Stable cell membrane labeling. (
  • Due to genetic heterogeneity, some cells cross faster. (
  • Most mitochondrial assays require either isolating the organelle, thus disrupting the intracellular signaling or using intact cells with average measurements for the entire population neglecting the ability to distinguish cell heterogeneity. (
  • However, lack of physiologic salts can impair cell viability over prolonged periods, and the dye aggregates will gradually form even in the absence of salts 1-3 . (
  • Illinois has joined the growing ranks of states limiting how police may use cell-site simulators , invasive technology devices that masquerade as cell phone towers and turn our mobile phones into surveillance devices. (
  • But even older phones that don't have chips can be tracked by knowing the location of the cell towers they use to connect to a network," the group said. (
  • Localization may occur either via multilateration of radio signals between (several) cell towers of the network and the phone, or simply via GPS. (
  • The accuracy of network-based techniques is both dependent on the concentration of cell base stations, with urban environments achieving the highest possible accuracy because of the higher number of cell towers, and the implementation of the most current timing methods. (
  • Here, cells are labeled either with fluorescent dyes or quantum dots, or transfected with plasmids coding for fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP). (
  • Choose passively loaded dyes for short- and long-term tracking or fluorescent fusion proteins for cell tracking over 5 or more days. (
  • Invitrogen CellLight fluorescent proteins can be expressed in a variety of cell types including numerous transformed cell lines, primary cells, stem cells and neurons. (
  • Porcine primary skeletal muscle cell transduced with CellLight Nucleus-GFP fluorescent proteins. (
  • Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. (
  • Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention. (
  • Identifying cell boundaries is a key step in automated image acquisition and analysis for high-content screening (HCS) assays. (
  • Zebrafish have become a powerful vertebrate model organism for drug discovery, cancer and stem cell research. (
  • Can the government use your cell phone records to track your physical location without first obtaining a warrant based on probable cause? (
  • The Justice Department, in responding to the ACLU lawsuit, identified 229 prosecutions since 2001 in which a judge had approved the government's request to obtain cellphone tracking data without making a finding of probable cause. (
  • NEW YORK - If you are a U.S. resident who owns a cell phone, you should care about the outcome of a court case that "could well decide whether the government can use your cell phone to track you - even if it hasn't shown probable cause to believe it will turn up evidence of a crime. (
  • Civil liberties groups are asking the court to require that the government show probable cause before it can track your whereabouts. (
  • Back in 2007, the U.S. government applied for court permission to obtain information about the location of an individual's cell phone, without showing probable cause that tracking the individual would turn up evidence of a crime. (
  • A number of civil liberties groups, on behalf of plaintiffs in the case, filed a friend-of-the-court brief in support of the district court decision, arguing that district courts must require the government to show probable cause before permitting the government to obtain information about the location of a cell phone. (
  • Batard P, Monier M-N, Fortunel N, Ducos K, Sansilvestri - Morel P, Phan T-H, Hatzfeld A, Hatzfeld J. (2000) TGF-beta 1 maintains the hematopoitic immaturity by a reversible negative control of cell cycle and induces CD 34 antigen up-modulation. (
  • Cellular imaging using magnetodendrimers: application to human stem cells and neoplastic cells in vivo. (
  • This tracking has given us detailed information for the commonly used endpoints of 5, 7, and 14 days that is unclouded by cellular aggregation. (
  • However, these techniques have drawbacks such as they may influence cellular properties, are radioactive and or quickly lose their tracking ability. (
  • Such dynamically tunable hydrogels can be utilized as in vitro cellular micro-environments that can exert time-dependent stimuli to mechanically regulate target cells. (
  • It is expected that in vivo cellular imaging will routinely be used to provide a surrogate biomarker for certain cell therapy and drug trials. (
  • Polyamines are ubiquitous small molecules involved in many normal cellular functions, including transcribing and translating genes, regulating ion channels and cell-to-cell interactions, and powering cell growth and replication. (
  • Once chemotherapy was paired with a genetic therapy to suppress the labeled cells, the tumors shrank back into residual vestiges. (
  • From cell-site simulators in New York to facial recognition devices in San Diego , law enforcement surveillance technologies are spreading across the country like an infectious disease. (
  • The Desert Sun of Palm Springs made a similar request to the San Bernardino County Sheriff's Department, which said it had to maintain secrecy even though the newspaper found information online about cell site simulators. (
  • 9. After incubation, Tag-it Violet™ labeled cells are ready for downstream applications or analysis. (
  • Those cells can be removed selectively from the chip and their mutations studied. (
  • (b) Adoptively transferred T cells selectively home to the pancreas in a pre-diabetic mouse model. (
  • The oldest and only clinically established method is leukocyte scintigraphy, which enables a (semi)quantitative assessment of cell assemblies and, thus, the localization of inflammation foci. (
  • b ) Fluorescence microscopy shows intracytoplasmic localization of PFC-dye (BODIPy, red) in mouse T cells, where the nucleus is blue (Hoechst dye) and the cell surface is stained with CD4- FITC antibody (green). (
  • The critical role that acto-myosin complexes play in the morphological transition was verified by the treatment of cells with myosin II inhibitor (blebbistatin) and the fluorescence localization of focal adhesion contacts. (
  • After two weeks, tumors that were treated with the therapeutic stem cells had shrunk by 80 percent. (
  • They discovered that all tumors contain at least a few labeled cells, presumably the cancer's stem cells. (
  • Tumors also contained many unlabeled cells, which could be killed off with standard chemotherapy, but the tumors quickly returned. (
  • Previously, Clevers and his team had shown that a genetic marker that labels healthy gut stem cells also labels stem cells in benign intestinal tumors, which are precursors of cancer. (
  • The experiment yielded single-color tumors consisting of several cell types, suggesting that each tumor originated from a single stem cell. (
  • As the tumors became more aggressive, they were more likely to produce more stem cells that can divide indefinitely and less likely to produce differentiated cells, which can only divide a limited number of times. (
  • 3. Prepare a 5 µM working solution by diluting 1 µL of 5 mM Tag-it Violet™ stock solution in 1 mL PBS for every 1 mL of cell suspension (or at an optimal working concentration as determined by titration). (
  • Law enforcement officers in Washington, D.C. violated the Fourth Amendment when they used a cell site simulator to locate a suspect without a warrant, a D.C. appeals court ruled on Thursday. (
  • In one of the rare court cases involving the device, the FBI acknowledged in 2011 that so-called cell site simulator technology affects innocent users in the area where it's operated, not just a suspect police are seeking. (
  • The advantage of the latter approach is that the label does not get lost during cell division and, thus, makes imaging of proliferating transplanted cells (e.g., tumor cells) possible. (
  • Ahrens ET, Flores R, Xu H et al (2005) In vivo imaging platform for tracking immunotherapeutic cells. (
  • Longitudinal MR tracking of mesioangioblasts was achieved following SPIO labeling of cells followed by high-resolution imaging. (
  • Delivering stem cells to specific tissues is a very big challenge, since basically all investigators have to shoot blindly," says Sanjiv Gambhir , director of Stanford's molecular imaging program who led the work published online today in Science Translational Medicine . (
  • With the aid of stimulus-responsive hydrogel substrates composed of ABA triblock copolymer micelles, we monitored the morphological dynamics of myoblast (C2C12) cells in response to an abrupt change in the substrate elasticity by live cell imaging. (
  • 2010;12(2):238-250 "In vivo imaging platform for tracking immunotherapeutic cells. (
  • The ruling came in a public records lawsuit brought by the American Civil Liberties Union, which had requested information on federal cases in which law enforcement had obtained cellphone tracking data without a warrant to track a user's whereabouts. (
  • This technique determines the location of the handset by putting its location by cell identification, signal strengths of the home and neighboring cells, which is continuously sent to the carrier. (
  • Another approach is to use a fingerprinting-based technique, where the "signature" of the home and neighboring cells signal strengths at different points in the area of interest is recorded by war-driving and matched in real-time to determine the handset location. (
  • This work, if replicated in humans, could finally allow scientists to verify if these cells are going where they're intended. (