The attitude of a significant portion of a population toward any given proposition, based upon a measurable amount of factual evidence, and involving some degree of reflection, analysis, and reasoning.
Any method used for determining the location of and relative distances between genes on a chromosome.
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
Communication through a system of conventional vocal symbols.
Organizations established by endowments with provision for future maintenance.
Persons whom one knows, likes, and trusts.
Activities concerned with governmental policies, functions, etc.
The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)
The protection of genetic information about an individual, family, or population group, from unauthorized disclosure.
The privacy of information and its protection against unauthorized disclosure.
Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.
A violation of the criminal law, i.e., a breach of the conduct code specifically sanctioned by the state, which through its administrative agencies prosecutes offenders and imposes and administers punishments. The concept includes unacceptable actions whether prosecuted or going unpunished.
Decisions made by the United States Supreme Court.
A belief or practice which lacks adequate basis for proof; an embodiment of fear of the unknown, magic, and ignorance.
Organized efforts to insure obedience to the laws of a community.
An instrument for reproducing sounds especially articulate speech at a distance. (Webster, 3rd ed)
Administrative units of government responsible for policy making and management of governmental activities.
Fields representing the joint interplay of electric and magnetic forces.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Communication between CELL PHONE users via the Short Message Service protocol which allows the interchange of short written messages.
The science or philosophy of law. Also, the application of the principles of law and justice to health and medicine.
The kind of action or activity proper to the judiciary, particularly its responsibility for decision making.
Electromagnetic waves with frequencies between about 3 kilohertz (very low frequency - VLF) and 300,000 megahertz (extremely high frequency - EHF). They are used in television and radio broadcasting, land and satellite communications systems, radionavigation, radiolocation, and DIATHERMY. The highest frequency radio waves are MICROWAVES.
Delivery of nursing services via remote telecommunications.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
Literary and oral genre expressing meaning via symbolism and following formal or informal patterns.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
The normal decreasing elasticity of the crystalline lens that leads to loss of accommodation.
Recording serial images of a process at regular intervals spaced out over a longer period of time than the time in which the recordings will be played back.
Non-invasive imaging of cells that have been labeled non-destructively, such as with nanoemulsions or reporter genes that can be detected by molecular imaging, to monitor their location, viability, cell lineage expansion, response to drugs, movement, or other behaviors in vivo.
Relating to the size of solids.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Sequential operating programs and data which instruct the functioning of a digital computer.
Agents of the law charged with the responsibility of maintaining and enforcing law and order among the citizenry.
Control which is exerted by the more stable organizations of society, such as established institutions and the law. They are ordinarily embodied in definite codes, usually written.
Control of drug and narcotic use by international agreement, or by institutional systems for handling prescribed drugs. This includes regulations concerned with the manufacturing, dispensing, approval (DRUG APPROVAL), and marketing of drugs.
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
The hollow, muscular organ that maintains the circulation of the blood.
A field of medicine concerned with developing and using strategies aimed at repair or replacement of damaged, diseased, or metabolically deficient organs, tissues, and cells via TISSUE ENGINEERING; CELL TRANSPLANTATION; and ARTIFICIAL ORGANS and BIOARTIFICIAL ORGANS and tissues.
The number of times the HEART VENTRICLES contract per unit of time, usually per minute.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.

Migration of intravenously grafted mesenchymal stem cells to injured heart in rats. (1/306)

The present study aimed to determine the role of tissue injury in migration of mesenchymal stem cells (MSCs) intravenously transplanted into heart and to establish experimental basis for improving stem cell therapy in its targeting and effectiveness. MSCs were isolated from bone marrow of male Sprague-Dawley rats and purified by density centrifuge and adhered to the culture plate in vitro. Female rats were divided randomly into four groups. Myocardial ischemia (MI) transplanted group received MSCs infusion through tail vein 3 h after MI and compared with sham-operated group or normal group with MSCs infusion, or control group received culture medium infusion. MI was created in female rats by ligating the left anterior descending coronary artery. The heart was harvested 1 week and 8 weeks after transplantation. The characteristics of migration of MSCs to heart were detected with expression of sry gene of Y chromosome by using fluorescence in situ hybridization (FISH). Ultrastructural changes of the ischemic myocardium of the recipient rats were observed by transmission electron microscope (TEM). One week or 8 weeks after transplantation, sry positive cells were observed in the cardiac tissue in both of MI transplanted group and sham-operated group, the number of sry positive cells being significantly higher in MI transplanted group (P<0.01). No significant difference was found in the number of sry positive cells between 1 week and 8 weeks after transplantation. No sry positive cells were observed in the hearts of control and normal group. In addition, the ultrastructure of some cells located in the peri-infarct area of MI rats with MSCs transplantation was similar to that of MSCs cultured in vitro. These results indicate that MSCs are capable of migrating towards ischemic myocardium in vivo and the fastigium of migration might appear around 1 week after MI. The tissue injury and its degree play an important role in the migration of MSCs.  (+info)

Environmental enrichment requires adult neurogenesis to facilitate the recovery from psychosocial stress. (2/306)


Non-invasive cell tracking in cancer and cancer therapy. (3/306)

Cell-based therapy holds great promise for cancer treatment. The ability to non-invasively track the delivery of various therapeutic cells (e.g. T cells and stem cells) to the tumor site, and/or subsequent differentiation/proliferation of these cells, would allow better understanding of the mechanisms of cancer development and intervention. This brief review will summarize the various methods for non-invasive cell tracking in cancer and cancer therapy. In general, there are two approaches for cell tracking: direct (cells are labeled with certain tags that can be detected directly with suitable imaging equipment) and indirect cell labeling (which typically uses reporter genes approach). The techniques for tracking various cell types (e.g. immune cells, stem cells, and cancer cells) in cancer are described, which include fluorescence, bioluminescence, positron emission tomography (PET), single-photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI). Non-invasive tracking of immune and stem cells were primarily intended for (potential) cancer therapy applications while tracking of cancer cells could further our understanding of cancer development and tumor metastasis. Safety is a major concern for future clinical applications and the ideal imaging modality for tracking therapeutic cells in cancer patients requires the imaging tags to be non-toxic, biocompatible, and highly specific. Each imaging modality has its advantages and disadvantages and they are more complementary than competitive. MRI, radionuclide-based imaging techniques, and reporter gene-based approaches will each have their own niches towards the same ultimate goal: personalized medicine for cancer patients.  (+info)

In vivo molecular imaging using nanomaterials: general in vivo characteristics of nano-sized reagents and applications for cancer diagnosis. (4/306)


Non-invasive imaging of human embryonic stem cells. (5/306)

Human embryonic stem cells (hESCs) hold tremendous therapeutic potential in a variety of diseases. Over the last decade, non-invasive imaging techniques have proven to be of great value in tracking transplanted hESCs. This review article will briefly summarize the various techniques used for non-invasive imaging of hESCs, which include magnetic resonance imaging (MRI), bioluminescence imaging (BLI), fluorescence, single-photon emission computed tomography (SPECT), positron emission tomography (PET), and multimodality approaches. Although the focus of this review article is primarily on hESCs, the labeling/tracking strategies described here can be readily applied to other (stem) cell types as well. Non-invasive imaging can provide convenient means to monitor hESC survival, proliferation, function, as well as overgrowth (such as teratoma formation), which could not be readily investigated previously. The requirement for hESC tracking techniques depends on the clinical scenario and each imaging technique will have its own niche in preclinical/clinical research. Continued evolvement of non-invasive imaging techniques will undoubtedly contribute to significant advances in understanding stem cell biology and mechanisms of action.  (+info)

Non-surgical stem cell delivery strategies and in vivo cell tracking to injured myocardium. (6/306)


Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging. (7/306)


Tracking and quantitation of fluorescent HIV during cell-to-cell transmission. (8/306)


Achieving complete understanding of any living thing inevitably requires thorough analysis of both its anatomic and dynamic properties. Live-cell imaging experiments carried out to this end often produce massive amounts of time-lapse image data containing far more information than can be digested by …
C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with latrunculin A. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video2 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.. ...
Quantification of changes in intracellular free Ca2+ concentrations [Ca2+]i is fundamental to the understanding of the physiology of single cells in response to both environmental and endogenous stimuli. Here we present easy to use freeware that allows especially the evaluation of [Ca2+]i signals in complex and mixed cultures. The program CaFFEE (Calcium Fluorescent Flash Evaluating Engine) enables the user to evaluate the response of hundreds of cells to treat-ments that influence [Ca2+]i. CaFFEE processes large quantities of image data, automatically identifies individual cells in mixed, heterogeneous populations, and evaluates their fluorescence signal. All data are exported in spreadsheet format, and data on thousands of cells can be batch-processed. Moreover, the program optimizes the visual representation of time-lapse image data for user-guided data exploration (setting of parameters for semi-automated data processing). The freeware allows the standardized and transparent processing of ...
TWO new customisation tools for its IX3 inverted microscopes deliver better multi-well time-lapse images and more stable focus during long studies, says Olympus. The IX3-ZDC2 module provides focal drift compensation for accurate focus stability during long time-lapse experiments. Updated CellSens…. Read more →. ...
The purposeful, synchronised travel paths of the hundreds of thousands of bacteria are captured in a brilliantly colourful time-lapse image
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
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C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.. ...
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TY - JOUR. T1 - Cellular uptake of folate-conjugated lipophilic superparamagnetic iron oxide nanoparticles. AU - Woo, Kyoungja. AU - Moon, Jihyung. AU - Choi, Kyu Sil. AU - Seong, Tae Yeon. AU - Yoon, Kwon Ha. PY - 2009/5. Y1 - 2009/5. N2 - We prepared five folate-conjugated lipophilic superparamagnetic iron oxide nanoparticles (F5-Liposuperparamagnetic iron oxide nanoparticles(SPIONs), 5.5 and 11 nm) and investigated their cellular uptake with KB cells, which is one of the representative folate-receptor over-expressing human epidermoid carcinoma cells, using MRI. The cellular uptake tests with the respective 5.5 and 11 nm F5-LipoSPIONs at a fixed particle concentration showed appreciable amount of receptor-mediated uptakes and the specificity was higher in 5.5 nm SPIONs, due to its higher folic acid (FA) density, without inhibition. However, the numbers of the particles taken up under FA inhibition were similar, irrespective of their sizes.. AB - We prepared five folate-conjugated lipophilic ...
In recent years, nanotechnology has emerged as one of the most promising fields for biomedical applications and healthcare. The use of nanoparticles has earned much attention due to their small size, high surface area to volume ratio along with surface charge, and their optical and semiconducting properties. Superparamagnetic iron oxide nanoparticles (SPIONs), which show superparmagnetism, have been reported to be reliable and safe for many biological applications.In the present study, 10nm SPION were loaded with anticancer drug, Docetaxel. Docetaxel conjugation to SPION was confirmed by FTIR, UV-vis spectroscopy and gel electrophoresis. SPIONs uptake by cells was investigated using Prussian blue dye staining.In vitro drug release was studied which showed 55% drug release in 24 h. Drug loaded SPION were then tested on Mammary tumor cells (4T1) and showed 40% cell viability with 0.1M Docetaxel conjugated to SPION. Our results shows that Docetaxel-SPIONs can reduce the amount of required anticancer drug
Page contains details about c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles :
We demonstrate that fatty acid modified iron oxide prolonged retention of the contrast agent in the polymer matrix during degradative release of drug. Antibody-fatty acid surface modification facilitated cellular targeting and subsequent internalization in cells while inducing clustering of encapsulated fatty-acid modified superparamagnetic iron oxide during particle formulation. This induced clustered confinement led to an aggregation within the nanoparticle and, hence, higher transverse relaxivity, r2, (294 mM−1 s−1) compared with nanoparticles without fatty-acid ligands (160 mM−1 s−1) and higher than commercially available superparamagnetic iron oxide nanoparticles (89 mM−1 s−1). ...
Adipose-derived stem cells (ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide (SPIO) nanoparticles are critical for magnetic resonance (MR) tracking of implanted cells. Hypoxia is a powerful stimulus for angiogenic activity of ASCs. In this study, we investigated whether therapeutic potency could be enhanced by implantation of hypoxia-preconditioned SPIO-labeled ASCs (SPIOASCs) into the infarcted myocardium. ASCs and SPIOASCs were cultured under 2% O2 (hypoxia) or 95% air (normoxia). Cells were intramyocardially injected into the infarcted myocardium after 48-h culture. We found that hypoxia culture increased the mRNA expression of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in ASCs and SPIOASCs. The VEGF protein in the conditioned medium was significantly higher in hypoxic ASCs and SPIOASCs than in normoxic ASCs and SPIOASCs. The capillary density and left ventricular contractile function in the
The density of proteins in solution is an important fundamental biophysical quantity that has not been accurately measured yet. This has led to extensive debate in the literature regarding the precise density value of proteins. This rough density metric can often lead to inaccurate estimates on the other physicochemical and composite protein features, especially after their interactions with other materials such as nanoparticles. Researchers have now demonstrated a new technique using superparamagnetic iron oxide nanoparticles for magnetic levitation, which promises a more precisely measurement of the density of proteins in solution.
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Entering service in 1848, the Needle Gun was used extensively by Prussian forces during the Austro-Prussian and Franco-Prussian Wars.
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TY - JOUR. T1 - HO-1 gene overexpression enhances the beneficial effects of superparamagnetic iron oxide labeled bone marrow stromal cells transplantation in swine hearts underwent ischemia/ reperfusion. T2 - An MRI study. AU - Jiang, Yibo. AU - Chen, Lijuan. AU - Tang, Yaoliang. AU - Ma, Genshan. AU - Shen, Chengxing. AU - Qi, Chunmei. AU - Zhu, Qi. AU - Yao, Yuyu. AU - Liu, Naifeng. PY - 2010/5/1. Y1 - 2010/5/1. N2 - To determine the effect of intracoronary transfer of superparamagnetic iron oxide (SPIO) labeled heme oxygenase-1 (HO-1) overexpressed bone marrow stromal cells (BMSCs) in a porcine myocardial ischemia/ reperfusion model. Cell apoptosis was assayed and supernatant cytokine concentrations were measured in BMSCs that underwent hypoxia/reoxygen in vitro. Female miniswines that underwent 1 h LAD occlusion followed by 1 h reperfusion were randomly allocated to receive intracoronary saline (control), 1 × 107 SPIO-labeled BMSCs transfected with pcDNA3.1-Lacz plasmid (Lacz-BMSCs), ...
Tracking vaccine components from the site of injection to their destination in lymphatic tissue, and simultaneously monitoring immune effects, sheds light on the influence of vaccine components on particle and immune cell trafficking and therapeutic efficacy. In this study, we create a hybrid particle vaccine platform comprised of porous silicon (pSi) and superparamagnetic iron oxide nanoparticles (SPIONs). The impact of nanoparticle size and mode of presentation on magnetic resonance contrast enhancement are examined. SPION-enhanced relaxivity increased as the core diameter of the nanoparticle increased, while encapsulation of SPIONs within a pSi matrix had only minor effects on T2 and no significant effect on T2* relaxation. Following intravenous injection of single and hybrid particles, there was an increase in negative contrast in the spleen, with changes in contrast being slightly greater for free compared to silicon encapsulated SPIONs. Incubation of bone marrow-derived dendritic cells (BMDC) with
Targeted radioisotope-guided sentinel lymph node dissection (sLND) has shown high diagnostic accuracy in prostate cancer (PCa). To overcome the downsides of the radioactive tracers, magnetometer-guided sLND using superparamagnetic iron oxide nanoparticles (SPIONs) was successfully applied in PCa. This prospective study (SentiMag Pro II, DRKS00007671) determined the diagnostic accuracy of magnetometer-guided sLND in intermediate- and high-risk PCa. Fifty intermediate- or high-risk PCa patients (PSA≥10 ng/ml and/or Gleason score ≥7; median PSA 10.8 ng/ml, IQR 7.4–19.2 ng/ml) were enrolled. After intraprostatic SPIONs injection a day earlier, patients underwent magnetometer-guided sLND and eLND, followed by radical prostatectomy. SLNs were detected in vivo and in ex vivo samples. Diagnostic accuracy of sLND was assessed using eLND as the reference. SLNs were detected in all patients (detection rate 100%), with 447 SLNs (median 9, IQR 6–12) being identified and 966 LNs (median 18, IQR 15
Application of hyperthermia induced by superparamagnetic iron oxide nanoparticles in glioma treatment André C Silva1, Tiago R Oliveira1,2, Javier B Mamani1, Suzana MF Malheiros3,4, Luciana Malavolta1, Lorena F Pavon1, Tatiana T Sibov1, Edson Amaro Jr1,5, Alberto Tannús6, Edson LG Vidoto6, Mateus J Martins6, Ricardo S Santos6, Lionel F Gamarra11Instituto Israelita de Ensino e Pesquisa Albert Einstein, IIEPAE, São Paulo, Brazil; 2Instituto de FÃ-sica, Universidade de São Paulo, São Paulo, Brazil; 3Departament of Neurology and Neurosurgery, Universidade Federal de São Paulo, São Paulo, Brazil; 4Neuro-Oncology Program of Hospital Israelita Albert Einstein, São Paulo, Brazil; 5Instituto de Radiologia, Faculdade de Medicina; 6CIERMag-Instituto de FÃ-sica de São Carlos, Universidade de São Paulo, São Paulo, BrazilAbstract: Gliomas are a group of heterogeneous primary central nervous system (CNS) tumors arising from the glial cells. Malignant gliomas account for a majority of
In the past few years, the use of nanostructured materials in medical applications hasdramatically increased, both in the research phase and for clinical purposes, due to thepeculiar properties and the ability of such materials to interact at a similar scale withbiological entities. In this thesis, we developed tailored magnetic multifunctionalnanoparticles for diagnostic and therapeutic applications, such as detection ofbiomolecules, simultaneous enhanced magnetic resonance imaging (MRI), fluorescentvisualization and controlled drug release.For sensitive and selective detection of specific biomolecules, thermally blocked ironoxide nanoparticles with tailored magnetic properties were developed. The formation ofsuch nanoparticles has been studied both in terms of size and magnetic behavior in liquidsuspension or in polymer matrixes. These particles with narrow size distribution (averagediameter of 19 nm) were surface functionalized by antigen molecules and were used forthe detection of Brucella ...
Superparamagnetic iron oxide (SPIO) labeling efficiency and subsequent MRI tracking of native cell populations pertinent to pulmonary heart valve tissue engineering studies Article ...
Acute Oral Toxicity: In different studies, Prussian blue insoluble (14038-43-8) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments data in rodents, i.e. most commonly in rats for Prussian blue insoluble (14038-43-8). The different studies on target substance are summarized as below - In another experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance Prussian blue insoluble (14038-43-8). Acute oral toxicity study was done in rats using Prussian blue (14038-43-8).No mortality was observed at dose 8000mg/kg bw.Hence LD50 was considered to be ,8000 mg/kg body weight,when rats were treated with Prussian blue (14038-43-8)orally. Also these results are further supported by the experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance ...
Gene therapy shows promise for individualized cancer therapies. Compared to conventional treatments, this approach offers a variety of advantages, including a multiplicity of gene targets that define tumor clinical behavior, high specificity, low off target toxicity, and suppression of drug resistance. However, the lack of efficient delivery strategies for gene-specific agents and preclinical models for evaluation of the efficacy of gene-based therapeutics has impeded its clinical application. Nanoparticles provide a flexible platform for fabricating novel delivery vehicles capable of transporting gene-specific therapeutic agents to target tumors. Among the various types of nano-carriers for cancer therapy, superparamagnetic iron oxide nanoparticles have emerged as a leading candidate as they have a host of advantages compared to other formulations. The iron oxide core facilitates the condensation of other molecules to it that confer biological properties that promote stability during transport ...
MPI (not to be confused with myocardial perfusion imaging, a radiology method for the heart) is a new medical imaging technology that maps superparamagnetic iron oxide nanoparticles that are injected into the blood stream. The first proof-of-principle study of this method was published in 2005. MPIs main advantages are that its more detailed than traditional imaging techniques, and it does not use any radiation. It produces images quickly, in comparison to other radiation-free techniques such as MRI, which is also more expensive.. These advancements in medical technology are available in health care settings today. Researchers at universities and companies are investigating other emerging technologies that could make their way into the hospital setting in the next few years. The upcoming decade may bring even more interesting advancements. This makes it an exciting time to become a radiology technician and learn to apply some of these and other technologies.. Photo Source: ...
Time-Lapse Reflection Imaging of Matrix Assembly - Collagen, type I, or pepsin-solubilized intestinal submucosal, PSIS, was polymerized in 8-well chambered coverglass (Lab-Tek, Nalge Nunc Int.). The unstained 3-dimensional matrices were viewed with an inverted confocal laser-scanning microscope (BioRad MRC1024) using a 60X, oil immersion lens. Laser light of 488 nm illuminated the hydrated samples and the reflected light was detected with a photomultiplier tube using a blue reflection filter. Samples were maintained at 30-37°C on a heated stage. Time-lapse images of fibrillogenesis were recorded at 10-15 second intervals. For standardizing the scanning depth in the matrix, the Z position was kept in the range of 20 to 50 µm from the coverglass.. All material (text, images and movies) copyright Purdue University. No material may be reproduced in any form without the express permission of Purdue University. ...
The Prussian blue (cyanide-bridged, ordered double perovskite) analogue potassium imidazolium hexacyanoferrate, (C3N2H5)2K[Fe(CN)6], contains imidazolium cations encapsulated within a metal-cyanide framework. These are free to rotate in the intermediate- and high-temperature phases, but freeze into fixed orientatio
A PLA2-responsive and superparamagnetic iron oxide (SPIO) nanoparticle-loaded phospholipid micelle was developed. atherosclerosis 1 pancreatitis 2 acute sepsis 3 and CPI-268456 cancer.4 For example in prostate cancer PLA2 levels have been found to be up to 22-fold higher than physiological levels.5-7 Thus it has been suggested that PLA2 enzymes can serve as a novel diagnostic…. Read more ...
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The aim of this study was to design thiolated surface stabilized superparamagnetic iron oxide nanoparticles (TSS-SPIONs) for efficient internalization with high MRI sensitivity. TSS-SPIONs were developed by chelation between thiolated chitosan-thioglycolic acid (chitosan-TGA) hydrogel and iron ions (Fe(2+)/Fe(3+)). Likely, unmodified chitosan hydrogel SPIONs (UC-SPIONs) and uncoated SPIONs were used as control. Moreover, TSS-SPIONs were investigated regarding to their iron core size, hydrodynamic diameter, zeta potential, iron contents, molar relaxivities (r1 and r2), and cellular internalization. TSS-SPIONs demonstrated an iron oxide core diameter (crystallite size by XRD) of 3.1 ± 0.02 nm, a hydrodynamic diameter of 94 ± 20 nm, a zeta potential of +21 ± 5 mV, and an iron content of 3.6 ± 0.9 mg/mL. In addition, internalization of TSS-SPIONs into human endothelial progenitor cells (EPC) from umbilical cord blood was more than threefold and 17-fold higher in contrast to UC-SPIONs and SPIONs, ...
Studying the effect of particle size and coating type on the blood kinetics of superparamagnetic iron oxide nanoparticles Farnoosh Roohi, Jessica Lohrke, Andreas Ide, Gunnar Schütz, Katrin DasslerMR and CT Contrast Media Research, Bayer Pharma AG, Berlin, GermanyPurpose: Magnetic resonance imaging (MRI), one of the most powerful imaging techniques available, usually requires the use of an on-demand designed contrast agent to fully exploit its potential. The blood kinetics of the contrast agent represent an important factor that needs to be considered depending on the objective of the medical examination. For particulate contrast agents, such as superparamagnetic iron oxide nanoparticles (SPIOs), the key parameters are particle size and characteristics of the coating material. In this study we analyzed the effect of these two properties independently and systematically on the magnetic behavior and blood half-life of SPIOs.Methods: Eleven different SPIOs were synthesized for this study. In the first set
Limited research has suggested iron oxide nanoparticles (FeNP) have an inhibitory effect against several different genera of bacteria: Staphylococcus, Bacillus and Pseudomonas spp. In this study we looked at the effect of three different sets of Fe3O4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. Two of the tested NPs were SPIONs (Superparamagnetic Iron Oxide Nanoparticles). Exposure of cells to the SPIONs at concentrations up to 200 μg/ml resulted in an increase in biofilm biomass by 16 h under static conditions and a corresponding increase in cell density in the bulk liquid. In contrast, these biofilms had decreased levels of extracellular DNA (eDNA). Fe(II) levels in the supernatants of biofilms formed in the presence of FeNPs exceeded 100 μM compared with 20 μM in control media without cells. Spent cell supernatants had little effect on Fe(II) levels. Cells also had an effect on the aggregation behavior of these nanoparticles. SPIONs incubated with cells
Completely water dispersible and highly monodispersed superparamagnetic iron oxide nanoparticles (SPIONs) were prepared. The surface of SPIONs was modified with dual-crosslinked amine activated dextran (AMD) and chemical cleavage of AMD on SPIONs was carried out by ethylenediamine hydrochloride (EDA). Transmission electron microscopy (TEM) revealed that individual SPIONs were completely separated in water and the average diameter of resulting nanoparticles was 4.4 nm.. ...
Spinal cord injury (SCI) is a condition that results in significant mortality and morbidity. Treatment of SCI utilizing stem cell transplantation represents a promising therapy. However, current conventional treatments are limited by inefficient delivery strategies of cells into the injured tissue. In this study, we designed a magnetic system and used it to accumulate stem cells labelled with superparamagnetic iron oxide nanoparticles (SPION) at a specific site of a SCI lesion. The loading of stem cells with engineered SPIONs that guarantees sufficient attractive magnetic forces was achieved. Celý text » ...
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Sigma-Aldrich offers abstracts and full-text articles by [Bin Zhou, Dan Li, Jiesheng Qian, Zhengran Li, Pengfei Pang, Hong Shan].
Article Immunosensor Based on Gold Nanoparticles-multi-walled Carbon Nanotubes-chitosans Composite and Prussian Blue for Detection of Chlorpyrifos, in Sensors and Transducers Journal, Vol. 146, Issue 11, November 2012, pp.121-132
Invented by Sir John Herschel in 1841, this simple process gives a continuous-tone image of Prussian Blue using a sensitising.... ...
Pada kesempatan ini, Binmas mengajak warga agar bersama sama di wilayah untuk mengantisipasi curat, curas dan curanmor dan congkel spion, serta selalu mewaspadai dan mencegah terjadinya curanmor curas dan curat dilingkungan kawasan pemukiman warga optimalkan pam swakarsa.. Kemudian Binmas mengimbau agar memantau dan mengawasi segala bentuk guantibmas terhadap lingkungan di sekitarnya. Apabila terjadi guantibmas agar segera menghubungi pihak Kepolisian terdekat atau polsubsektor terdekat dan tidak main hakim sendiri apabila ada pelaku tindak pidana tertangkap tangan. Tujuan patroli dialogis ini untuk mencegah bertemunya niat dan kesempatan di lokasi-lokasi rawan guantibmas serta menggali informasi yang ada di masyarakat sebagai masukan dalam melaksanakan tugas polri untuk terciptanya wilayah yang kondusif.. ( ...
Å hekle på glidelås er så lettvindt. Denne lille mappa kan bli det meste. Teknikken er veldig grei å kunne. To crochet direct ...
Å hekle på glidelås er så lettvindt. Denne lille mappa kan bli det meste. Teknikken er veldig grei å kunne. To crochet direct ...
Adferdsproblemer og andre fysiske eller psykiske problemer kan være knyttet til proteinintoleranse(matintoleranse). Npif arbeider for at kunnskapen om dette skal bli kjent og anerkjent.
Adferdsproblemer og andre fysiske eller psykiske problemer kan være knyttet til proteinintoleranse(matintoleranse). Npif arbeider for at kunnskapen om dette skal bli kjent og anerkjent.
The magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) have made them useful tools in a variety of applications ranging from drug delivery to magnetorheologic valving in microfluidic systems. The structure of SPIONs is typically characterized by methods such as XRD, XAFS, SEM and high resolution TEM while the SPION magnetic properties are evaluated relative to bulk values using SQUID. These methods characterize the state of the nanoparticles; however, the methods provide limited information about the behavior of the particles in solution. Here we demonstrate that electron paramagnetic resonance (EPR) reveals the solution behavior of 13 nm SPIONs suspended in a methanol:glycerol solution as well as environmental changes around the SPIONs. For example, characteristic temperature-dependent changes in the EPR spectra detail orientation changes of the SPIONs. The data presents a new opportunity for observing the in vitro, in vivo, and solution behavior of magnetite SPIONs.
A comprehensive literatures update of clinical researches of superparamagnetic resonance iron oxide nanoparticles for magnetic resonance imaging
Abstract Background In order to increase understanding of how infused cells work, it becomes important to track their initial movement, localization, and engraftment efficiency following transplantation. However, the available in vivo cell tracking techniques are suboptimal. The study objective was to determine the biodistribution of intravenously administered Indium-111 (In-111) oxine labeled human umbilical tissue-derived cells (hUTC) in a rat model of transient middle cerebral occlusion (tMCAo) using single photon emission computed tomography (SPECT). Methods Rats received 3 million In-111 labeled hUTC (i.v.) 48 hrs after tMCAo. Following the administration of either hUTC or equivalent dose of In-111-oxine (18.5 MBq), animals underwent SPECT imaging on days 0, 1, and 3. Radioactivity in various organs as well as in the stroke area and contralateral hemisphere was determined, decay corrected and normalized to the total (whole body including head) radioactivity on day 0. Immunohistochemical analysis
We demonstrate that 113Cd NMR is a potent technique to monitor the local electronic and structural states of the Prussian blue electrode during Li+ intercalation, providing an atomic-scale insight into the reaction mechanism.
Figure 1. Time-lapse images of spindles assembled in Xenopus egg extract around a bead coated with RCC1, the guanine nucleotide exchange factor for Ran (green, upper panel) or a bead coated with chromatin (blue, lower panel). This experiment shows that a single protein (RCC1) can induce spindle assembly, but the bead oscillates within the spindle.. Size Control. Size varies widely in biology at many levels: the animal, the cells that make up the animal, and the contents of the cells, but we dont understand how scaling occurs so that everything fits and functions properly. Correct organelle size is crucial for cell function, architecture, and division, but the control systems that a cell uses to regulate the size of its organelles are virtually unknown. Macromolecular cellular assemblies like the ribosome or nuclear pore self assemble from a fixed number of subunits and do not vary in size. In contrast, many complex organelles adjust in size to suit a particular cellular environment. Loss of ...
Downloadable! This paper provides a documentation of the ifo Prussian Economic History Database (iPEHD), a county-level database covering a rich collection of variables for 19th-century Prussia. The Royal Prussian Statistical Office collected these data in several censuses over the years 1816-1901, with much county-level information surviving in archives. These data provide a unique source for micro-regional empirical research in economic history, enabling analyses of the importance of such factors as education, religion, fertility, and many others for Prussian economic development in the 19th century. The service of iPEHD is to provide the data in a digitized and structured way.
posted at the central post office for delivery in Port Louis, and two pence for delivery elsewhere on the island.. Only 12 examples of the 1847 2 pence stamp are known still to exist. One unused 1847 2 pence stamp is held at the British Library as a part of the Tapling Collection. The rarity and hence great value of these Mauritian stamps, the rarest realising up to about £1 million at auction, is such that they are often known as the Crown Jewels ...
Crystal Structures of Photo-induced Phase and Rapidly-cooled Phase in Rb_,0.73,Mn[Fe(CN)_6]_,0.91,・1.4H_2O Prussian Blue Analog (2006 ...
Nalotimagene carmaleucel, an ex vivo cell therapy for immune reconstitution in patients with high-risk acute leukaemia who are undergoing haplo-identical
SSEA4, PerCP-eFluor™ 710, clone: eBioMC-813-70 (MC-813-70), eBioscience™ 25 Tests; PerCP-eFluor™ 710 SSEA4, PerCP-eFluor™ 710, clone:...
Bakgrunn: Kama muta (sanskrit: å bli beveget av kjærlighet) er et fagbegrep for det vi på norsk kaller å bli rørt. Navnet fanger essensen i denne emosjonen som oppstår som en reaksjon på å oppleve eller være vitne til at ...
Cell tracking[edit]. By tagging therapeutic cells with iron oxide nanoparticles, MPI allows them to tracked throughout the body ... Imaging can be used to improve the success of stem cell therapy by following the movement of these cells in the body.[3] The ... Tracking short-term biodistribution and long-term clearance of SPIO tracers in magnetic particle imaging. Keselman P, Yu E, ... "Magnetic Particle Imaging tracks the long-term fate of in vivo neural cell implants with high image contrast". Scientific ...
Tracking telomerase. „Cell". 116 (2 Suppl), s. S83-6, 1 p following S86, Jan 2004. PMID: 15055591. ... Cell". 123 (6), s. 1121-31, Dec 2005. DOI: 10.1016/j.cell.2005.11.020. PMID: 16360040. ... Cell". 51 (6), s. 887-98, Dec 1987. PMID: 3319189. *EH. Blackburn, CW. Greider, E. Henderson, MS. Lee i inni. Recognition and ... Mol Cell". 24 (3), s. 423-32, Nov 2006. DOI: 10.1016/j.molcel.2006.10.020. PMID: 17070718. ...
"High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro ... December 1997). "Insulin has a limited effect on the cell cycle progression in 3T3 L1 fibroblasts". Molecules and Cells. 7 (6 ... Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples ... October 2004). "'Proliferative' and 'synthetic' airway smooth muscle cells are overlapping populations". Immunology and Cell ...
PMCID: PMC3141927 Kircher MF, Gambhir SS, Grimm J (2011). "Noninvasive cell-tracking methods". Nat Rev Clin Oncol. 8 (11): 677- ... PMCID: PMC122480 Adonai N., Nguyen K., Walsh J., Iyer M., Toyokuni T., Phelps M.E., McCarthy D., Gambhir S.S. Ex Vivo Cell ... PET reporter gene technology, multimodality reporter genes, imaging of gene/cell therapies, imaging of the immune system, ... 2016, early ed.). Molecular profiling of single circulating tumor cells from lung cancer patients. PNAS Weissleder R, Schwaiger ...
Berns, GS; Berns, MW (1982). "Computer-based tracking of living cells". Exp. Cell Res. 142: 103-109. doi:10.1016/0014-4827(82) ... "Laser microsurgery in cell and developmental biology". Science. 213: 505-513. doi:10.1126/science.7017933.. ...
While Carpenter's lawyers argued that the cell phone tracking data should have been subject to the exclusionary rule and thrown ... data or cell site location information (CSLI). CSLI is cell phone information captured by nearby cell towers; and this ... In her dissent, Judge Stranch states, "this case involves tracking physical location through cell towers and a personal phone, ... National Association of Criminal Defense Lawyers (June 7, 2016). "Cell Phone Location Tracking" (PDF). ...
"E911 Turns Cell Phones into Tracking Devices". WIRED. 6 January 1998. Retrieved 2018-10-05. "Sprint, Alltel, USC fined for ... Advanced Mobile Location eCall Emergency Medical Dispatcher GPS tracking Mobile phone tracking Next Generation 9-1-1 Northern ... When a new account is created, the address is located in the Master Street Address Guide to track the proper Emergency Service ... E911 Phase 1: Wireless network operators must identify the phone number and cell phone tower used by callers, within six ...
Nunez, Toni (November 2013). "Tracking criminals with cell tower analysis" (PDF). Cityscape: A Journal of Policy Development ... "Demos: Tracking the H1N1 (Swine Flu) Pandemic". Oculus Info Inc. Archived from the original on 13 November 2012. Retrieved 29 ... It has been used to analyze data from sources as diverse as downhill skiing, influenza monitoring, and GPS wildlife tracking. A ... Lawyers and privacy campaigners have questioned whether innocent individuals may be tracked by the software, which has been ...
"DOJ Emails Show Feds Were Less Than "Explicit" With Judges On Cell Phone Tracking Tool". American Civil Liberties Union. ... Gillum, Jack (22 March 2014). "Police keep quiet about cell-tracking technology". Associated Press, Yahoo News. Retrieved 3 ... Rivero, Daniel (18 March 2015). "It's now a trend: third court orders the release of phone-tracking Stingray documents". fusion ... Nathan Freed Wessler (3 March 2014). "Police Hide Use of Cell Phone Tracker From Courts Because Manufacturer Asked". American ...
"Attention, Shoppers: Store Is Tracking Your Cell". NY Times. Retrieved 18 August 2017. Paul C. Box, Joseph C. Oppenlander (1976 ... This metric looks at the number of people entering a store who had visited the store previously by tracking the unique WiFi ... Through WiFi counting, owners can track the time a person carrying a smartphone entered and left the venue. This metric tracks ... "How Wi-Fi Can Count the People in a Room Without Tracking Their Phones". Popular Mechanics. Retrieved 17 August 2017. "Security ...
"Cell Phone Location Tracking Public Records Request". American Civil Liberties Union. 2013-03-25. Retrieved 2014-07-22. " ... It is also possible to get greater resolution of a phone's location by combining information from a number of cells surrounding ... "FBI taps cell phone mic as eavesdropping tool". CNET. Retrieved 2010-06-24. The FBI appears to have begun using a novel form of ... the location, which cells routinely communicate (to agree on the next handoff-for a moving phone) and measuring the timing ...
Dead Cell (track listing). Papa Roach. DreamWorks Records. 2001.CS1 maint: others in cite AV media (notes) (link) "Modern Rock ... Before I Die (track listing). Papa Roach. Eleven Seven Music. 2013.CS1 maint: others in cite AV media (notes) (link) Love, Bret ...
Cell-phone tracking software. $1 billion. [23]. 000000002001-03-01-0000March 2001. FleetAdvisor. Fleet management software. Not ... Tracking devices - OmniTRACS is a two-way satellite communications and geolocation trailer tracking technology designed for the ... Cell phone user interface tools and apps. $36 million. [27]. 000000002005-08-01-0000August 2005. Elata. Mobile content software ... The system is used for voice telephony via hand-held satellite phones, asset tracking and data transfer using mobile satellite ...
This information can be used to track a cell phone user's movements throughout the day. as well.(p. 18) The government ... "Supreme Court Passes on Cell Phone Tracking Case". Roll Call. Retrieved 2015-11-10. David Bryan Sentelle, C.J. (11 June 2014 ... As a cell phone caller moves, their connection is automatically handed-off to another cell site that is close by, as needed. ... a cell phone service provider. Mobile phone tracking data had helped place the defendant in this case at the scene of several ...
"Antimicrobial peptides and cell processes tracking endosymbiont dynamics". Philosophical Transactions: Biological Sciences. 371 ... Bacteriomes contain specialized cells, called bacteriocytes, that provide nutrients and shelter to the bacteria while ...
The tracking area is the LTE counterpart of the location area and routing area. A tracking area is a set of cells. Tracking ... Within a location area, each base station is assigned a distinct "cell identifier" (CI) number, see also Cell Global Identity. ... Tracking area updates are performed periodically or when the UE moves to a tracking area that is not included in its TA list. ... The IMSI is sent as rarely as possible, to avoid it being identified and tracked. A key use of the TMSI is in paging a mobile ...
Using single cell lineage tracking and the Baxter algorithms her lab developed, cell morphology, movement, cell-cell ... "Transient inactivation of Rb and ARF yields regenerative cells from postmitotic mammalian muscle". Cell Stem Cell. 7 (2): 198- ... Chiu, C. P.; Blau, H. M. (1984-07-01). "Reprogramming cell differentiation in the absence of DNA synthesis". Cell. 37 (3): 879- ... By transiently alleviating these brakes on the cell cycle, post-mitotic cells are induced to divide, reconstituting a ...
Burrill DR, Inniss MC, Boyle PM, Silver PA (July 1, 2012). "Synthetic memory circuits for tracking human cell fate". Genes & ... "An alternative splicing network links cell-cycle control to apoptosis". Cell. 142 (4): 625-636. doi:10.1016/j.cell.2010.07.019 ... Cell. 122 (1): 33-43. doi:10.1016/j.cell.2005.05.008. PMID 16009131. S2CID 16841542. Retrieved May 6, 2015. CS1 maint: ... Cell. 159 (4): 925-39. doi:10.1016/j.cell.2014.10.002. PMC 4265554. PMID 25417166. Retrieved May 7, 2015. CS1 maint: ...
"Genetic tracking identifies cancer stem cells in patients". University of Oxford. 16 May 2014. Retrieved 16 May 2014. "'Biggest ... fat cells into brown, or "good," fat cells. Two compounds have already been shown to achieve this in human cells. 10 December ... Genetic mutations that drive cancer are tracked back to cancer stem cells in patients for the first time. 17 May - ... "Stem cells created from a drop of blood: DIY finger-prick technique opens door for extensive stem cell banking". Science Daily ...
In the past, Green Fluorescent Protein (GFP) was used to track movement inside cells. However, GFP does not light up well and ... By using quantum dots, which are more stable, researchers can now track proteins through cells undergoing different pathways. ... Baba, Koichi; Nishida, Kohji (2012). "Single-Molecule Tracking in Living Cells Using Single Quantum Dot Applications". ... researchers have developed a 3D tracking apparatus that can accurately map the path of proteins inside cells. Because quantum ...
de Almeida PE, van Rappard JR, Wu JC (September 2011). "In vivo bioluminescence for tracking cell fate and function". American ... Cell-based therapies focus on immunotherapies, mostly by engineering T cells. T cell receptors were engineered and 'trained' to ... A living "artificial cell" has been defined as a completely synthetic cell that can capture energy, maintain ion gradients, ... Since the number of T-cells are important for therapy persistence and severity, growth of T-cells is also controlled to dial ...
"Lasing within Live Cells Containing Intracellular Optical Microresonators for Barcode-Type Cell Tagging and Tracking". Nano ... This technique allows for the organizing and sorting of cells, the tracking of the movement of bacteria, and the changing of ... Enclosing an optical feedback structure in a cell has been demonstrated using cell vacuoles[12], as well as using fully ... means that surgeons can excise diseased or damaged cells without disturbing or damaging healthy surrounding cells in delicate ...
"Tracking the embryonic stem cell transition from ground state pluripotency". Development. The Company of Biologists. 144 (7): ... Here, he became a director of the Welcome Trust Centre for Stem Cell Research and later was the director of the new Wellcome ... He is notable for his pioneering work on the biology of embryonic stem cells. Austin Smith obtained his doctoral degree from ... In 1996, he was appointed director of the Centre, which became the Institute for Stem Cell Research under his leadership. He ...
"Meet the MacArthur 'genius' tracking the evolution of our cells". STAT. October 12, 2017. Retrieved 2017-11-01. "Gabriel ... "Tracking Antibody Diversity". NIH Director's Early Independence Award Highlights. National Institutes of Health. Retrieved 2017 ...
"Live Cell Imaging: Automated Cell Tracking and Analysis: Phasefocus Ptychography". Anon (2015). "Royal ...
The photovoltaic cells are thin-film with single axis tracking. "Queensland's biggest solar farm starts generating to grid". ...
"Trk signaling regulates neural precursor cell proliferation and differentiation during cortical development". Development. 134 ... cell-cell signaling. • positive regulation of brain-derived neurotrophic factor receptor signaling pathway. • collateral ... neurotrophin TRK receptor signaling pathway. • positive regulation of non-membrane spanning protein tyrosine kinase activity. • ... regulation of protein localization to cell surface. • regulation of receptor activity. • activation of phospholipase C activity ...
Bartkowska K, Paquin A, Gauthier AS, Kaplan DR, Miller FD (December 2007). "Trk signaling regulates neural precursor cell ... cell-cell signaling. • positive regulation of brain-derived neurotrophic factor receptor signaling pathway. • collateral ... neurotrophin TRK receptor signaling pathway. • positive regulation of non-membrane spanning protein tyrosine kinase activity. • ... regulation of protein localization to cell surface. • regulation of receptor activity. • activation of phospholipase C activity ...
"Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis". Proc. Natl. Acad. Sci. U.S.A. 103 (16 ... 2017). "Interspecies Chimerism with Mammalian Pluripotent Stem Cells". Cell. 168: 473-486.e15. PMID 28129541. doi:10.1016/j. ... Such animals, which are hence composed of a mixture of cells from more than one species, are called "chimera's"[95][96] One ... sickle cell anemia,[114] Parkinson's disease,[115][116] cancer,[117][118][119] diabetes,[120] heart disease[121] and muscular ...
He was tracked by his cell phone. He fatally shot himself after a 10-minute standoff in his hotel room, while members of the ... ends 10-minute standoff after telling marshal who tracked him from New Jersey that he could not face prospect of spending next ...
Photoelectric cells in a spectrophotometer device worn on the forehead measure the amount of each wavelength of light reflected ... Patient progress will be tracked using the same measure at the beginning and end of every neurotherapy session. Single photon ...
"Ash dieback: App developed to track diseased trees". BBC News. 29 October 2012. Retrieved 2 November 2012.. ... perforating the middle lamella but damage to either the plasmalemma or cell walls was not observed.[29] The disease is often ...
... dendritic cells and other cells including liver cells, fibroblasts, and adrenal gland cells.[93] Viral replication triggers ... decline in tracking indices of observed chimpanzee populations in the 420 km2 Lossi Sanctuary between 2002 and 2003.[243] ... doi:10.1016/j.cell.2014.10.006. PMC 4243531. PMID 25417101.. *^ a b c d e f g h Kühl A, Pöhlmann S (September 2012). "How Ebola ... liver cells, and several types of immune cells such as macrophages, monocytes, and dendritic cells are the main targets of ...
... and Th1 cells.[45] IL-1α stimulates increased skin cell activity and reproduction, which, in turn, fuels comedo development.[45 ... Pahwa M, Pahwa P, Zaheer A (July 2012). ""Tram track effect" after treatment of acne scars using a microneedling device". ... and accumulation of skin cells in the hair follicle.[1] In healthy skin, the skin cells that have died come up to the surface ... the increased production of oily sebum causes the dead skin cells to stick together.[10] The accumulation of dead skin cell ...
1: Olfactory bulb 2: Mitral cells 3: Bone 4: Nasal Epithelium 5: Glomerulus 6: Olfactory receptor cells ... వాసన పట్టు to scent, to catch the scent of, to trace, to track. వాసనవేయు or వాసనకొట్టు vāsana-vēyu. v. n. To smell, to emit an ...
The stable isotope signals in the layers of barnacle shells can potentially be used as a forensic tracking method for whales, ... Such barnacles feed by extending thread-like rhizomes of living cells into their hosts' bodies from their points of attachment. ... degrading to the condition of nothing more than sperm-producing cells.[15] ...
Depending on cell width, the links show above or next to the diamond. While technically possible to enter plain text, this is ... Tracking category[uredi kodo]. *Kategorija:Članki z neznano NFPA 704 kodo (40) ...
The workers had tracked down patients with rare diseases wiping out classic rod and cone photoreceptor function but preserving ... Further complexity arises from the various interconnections among bipolar cells, horizontal cells, and amacrine cells in the ... ON bipolar cells or inhibit (hyperpolarize) OFF bipolar cells. Thus, it is at the photoreceptor-bipolar cell synapse where ... which releases a neurotransmitter called glutamate to bipolar cells. Farther back is the cell body, which contains the cell's ...
... cell phone strap charms, and Disney character keychains. ... Test Track. *Odyssey Pavilion (The EPCOT Experience Center). ...
The association with track length and duration also varies, although longer track tornadoes tend to be stronger.[64] In the ... Multiple tornadoes produced by the same storm cell are referred to as a "tornado family".[21] Several tornadoes are sometimes ... Tornadic storms do not contain more lightning than other storms and some tornadic cells never produce lightning at all. More ... "Storm Track. 6 (3): 5-9. Archived from the original on 2012-06-19.. ...
... track and field), John Havlicek, Jerry Lucas, Bobby Knight, and Larry Siegfried (basketball), 2010 Olympics silver medalist ... engineering students and engineers from the Ford Motor Company and will seek to break the land speed record for hydrogen cell ... Larry Snyder in track and field, and Mike Peppe in swimming and diving. Hall of fame players, in pro-football, include Sid ... men's outdoor track & field, men's golf, men's gymnastics, men's fencing, women's rowing, co-ed fencing, and multiple ...
It has received regulatory approval for use as a treatment for non-small cell lung cancer,[6][4][7][8] although there is ... Afatinib, sold under the brand name Gilotrif among others, is a medication used to treat non-small cell lung carcinoma (NSCLC). ... May 2012). "Afatinib versus placebo for patients with advanced, metastatic non-small-cell lung cancer after failure of ... "Role of afatinib in the treatment of advanced lung squamous cell carcinoma". Clinical Pharmacology. 9: 147-157. doi:10.2147/ ...
The escape was set up by Vicky so that Leila would contact Sean, and Vicky could track Leila to Sean's location then and ... At the same time, Michael confronts Leila over the call made using the stolen cell phone. He then kills Leila's handler as he ... The team manages to track Sophia and Thomas to a warehouse and moves in to capture them, but Thomas mysteriously causes the ... Meanwhile, Sterling and President Martinez continue to track Sophia, though they know that someone in Lee's team is involved ( ...
The spirochetes may also induce host cells to secrete quinolinic acid, which stimulates the NMDA receptor on nerve cells, which ... The FDA has just fast-tracked a new one". Newsweek. 25 July 2017. Archived from the original on 25 December 2017. Retrieved 25 ... A hexavalent (OspA) protein subunit-based vaccine candidate VLA15 was granted fast track designation by the U.S. Food and Drug ... However, PCR tests are susceptible to false positive results, e.g. by detection of debris of dead Borrelia cells or specimen ...
They track it and find two locations it is used. Self and Bagwell go for one that is in a church; but they are not allowed ... Burrows' team arrives too and abduct Sandinsky while the duo take his cell phone, realizing that he has been in contact with ... Burrows' team arrives in Miami, where Scott has been tracked to. Self attempts to force a vote to depose Burrows as the boss ... Michael calls Christina again and she manages to track him. She arrives with her men; but this was revealed to be a ruse by ...
In hunting and tracking, terms such as dung, scat, spoor, and droppings normally are used to refer to non-human animal feces ... A combination of bile and bilirubin, which comes from dead red blood cells, gives feces the typical brown color.[1][2] ... and the body starts expelling bilirubin from dead red blood cells, its matter acquires the familiar brown color.[2] ... and the dead epithelial cells from the lining of the gut.[1] ...
cell nucleus. • cytosol. Biological process. • regulation of transcription, DNA-templated. • cell-cell signaling. • negative ... "Tracking progesterone receptor-mediated actions in breast cancer". Pharmacology & Therapeutics. 142 (1): 114-25. doi:10.1016/j ... epithelial cell maturation. • mammary gland development. • paracrine signaling. • lung alveolus development. • regulation of ... epithelial cell proliferation both in response to estrogen alone and in the presence of progesterone and estrogen. These ...
To avoid losing track of individual characters and plot threads in the story's large cast, editor Bob Harras maintained roughly ... Meanwhile, as Domino (who was placed in a fiery prison cell) works her charm on a familiar looking kid to exploit an ... Bob: Blue skinned, can create miniature duplicates from his blood cells.. *Russell: Possesses super speed. Often seen wearing a ...
Electrolysis cells can be either open cell or closed cell. In open cell systems, the electrolysis products, which are gaseous, ... it is implied that another student elsewhere is on a similar track, and may well repeat Todtman's efforts. ... the power input to the cell was equal to the calculated power leaving the cell within measurement accuracy, and the cell ... Groups that did report successes found that some of their cells were producing the effect, while other cells that were built ...
Fuentes would run the blood through a centrifuge, separating the blood plasma from the red blood cells. The cells would be re- ... IAAF was assured by Spanish prosecutors that no track and field athletes were involved.[58] On 23 September 2006, former ... If haematocrit levels (volume of red blood cells) got dangerously high, they would re-inject plasma as well, enhanced with EPO ... to dilute the red blood cells and avoid detection. The Barcelona lab did not identify any athlete responsible for any of the 99 ...
Examples include customer relationship management (CRM), human resources, and project tracking systems, such as Tracker Suite ...
When the bladder is fully distended the superficial cells become squamous (flat) and the stratification of the cuboidal cells ... Field Guide to Mammal Tracking in North America - James C. Halfpenny, Elizabeth Biesiot - Google Books. ... Field Guide to Mammal Tracking in North America - James C. Halfpenny - Google Books. Retrieved 30 November ... David Moskowitz (19 May 2010). Wildlife of the Pacific Northwest: Tracking and Identifying Mammals, Birds, Reptiles, Amphibians ...
If we consider a cell of the lattice formed, we can see that it is a rhombus with the four sides equal to d = p/sin α; (we have ... if the beats of one track correspond to where in space a black dot or line exists and the beats of the other track correspond ...
tracking. *coded wire tag. *Bird migration *flyways. *reverse migration. *Cell migration. *Fish migration *diel vertical ...
In oncology, the term nadir is used to represent the lowest level of a blood cell count while a patient is undergoing ... A satellite ground track represents its orbit projected to nadir on to Earth's surface. ...
If a fire occurs on the track, there is no flame or smoke to obstruct the view of fast approaching drivers, but this can also ... Fuel cell vehicle. *Hydrogen economy. *Hydrogen vehicle. *Hydrogen internal combustion engine vehicle ... Methanol was used by the CART circuit during its entire campaign (1979-2007). It is also used by many-short track organizations ... The gasoline-triggered fire created a dangerous cloud of thick black smoke that completely blocked the view of the track for ...
... only one cell released into the environment could exponentially grow into many thousands of cells over a short amount of time. ... Terrill, Ceiridwen (2007). Unnatural Landscapes: Tracking Invasive Species. University of Arizona Press. p. 240. ISBN 978-0- ... For organisms between 10 and 50 microns, such as certain types of phytoplankton, current regulations allow less than 10 cells ...
For example, the Canadian currency tactile feature is a system of raised dots in one corner, based on Braille cells but not ... Scientists track eye movements in glaucoma patients to check vision impairment while driving ... Two experimental treatments for retinal problems include a cybernetic replacement and transplant of fetal retinal cells.[71] ...
These included the Post Track and the Sweet Track. The Sweet Track, dating from the 39th century BC, is thought to be the ... Nearby on Lydiard Fields in Lydiard Tregoze is Johnson Matthey Fuel Cells, which in 2002 was the world's first production site ... The Herepath has a characteristic form which is familiar on the Quantocks: a regulation 20 m wide track between avenues of ...
Many methods tracking these types of changes have been developed using a variety of skeletal series. For instance, in children ... That as the body attempts to compensate for low iron levels by increasing red blood cell production in the young, sieve-like ...
Can the government use your cell phone records to track your physical location without first obtaining a warrant based on ... Can the government use your cell phone records to track your physical location without first obtaining a warrant based on ... EFF Related Content: Cell Tracking. Filter by Type - Any -. Deeplinks Blog. Document. Event. Legal Case. Press Release. ... and rejecting government applications for cell site location tracking information made without showing sufficient need for this ...
Can the government use your cell phone records to track your physical location without first obtaining a warrant based on ... Supreme Court Will Hear Significant Cell Phone Tracking Case. Today the Supreme Court announced it will review United States v ... Can the government use your cell phone records to track your physical location without first obtaining a warrant based on ... Read more about EFF to Supreme Court: No Real-Time Cell Phone Tracking Without a Warrant ...
"Theres no question that cell. phones and cell-phone records can be useful for police officers who. need to track the movements ... cell phone to track you - even if it hasnt shown probable cause to. believe it will turn up evidence of a crime." ... enforcement is tracking Americans cell phones in real time - without. the benefit of a warrant. Amid all the furor over the ... But even older phones that dont have chips can be tracked. by knowing the location of the cell towers they use to connect to a ...
Cell-based therapies may gain future importance in defeating different kinds of diseases, including cancer, immunological ... Cell-tracking approaches using optical methods are important for preclinical research. Here, cells are labeled either with ... Green Fluorescent Protein Progenitor Cell Superparamagnetic Iron Oxide Iron Oxide Particle Cell Tracking These keywords were ... tumor cells) possible.. In summary, there are several promising options for noninvasive cell tracking, which have different ...
... is tracking me. Using a $100 kit from Mologogo ... a write up of a low cost way to do your own cell phone tracking ... Our pals at Popular Science have a write up of a low cost way to do your own cell phone tracking - "…Jen, is tracking me. Using ... a $100 kit from Mologogo (with a $6-a-month data plan), Ive turned a prepaid cellphone into a GPS tracking device. Every few ...
The Justice Department does not have to turn over information on cases involving warrantless cellphone tracking if the cases ... requested information on federal cases in which law enforcement had obtained cellphone tracking data without a warrant to track ... The Justice Department does not have to turn over information on cases involving warrantless cellphone tracking if the cases ... identified 229 prosecutions since 2001 in which a judge had approved the governments request to obtain cellphone tracking data ...
... rejected the Obama administrations contention that the government is never required to get a court warrant to obtain cell-site ... Court Rebuffs Obama on Warrantless Cell-Site Tracking. A federal appeals court on Wednesday rejected the Obama administrations ... The data identifies the cell tower the customer was connected to at the beginning of a call and at the end of the call - and is ... The case that concluded Wednesday concerns historical cell-site location information, which carriers usually retain for about ...
Is Your Cell Phone Accelerometer Tracking You?. By Sean Michael Kerner , October 15, 2013 Page 1 of 1 ... Your Phone Is Tracking You--Should You Care? Sean Michael Kerner is a senior editor at Follow him on Twitter ... The concern is that, unlike other forms of tracking, this is one that a user could not opt out of or shut down. ... last week on SFGate about some new Stanford University research into how sensors on smartphones could be used for tracking ...
Second, NOG keeps the metastatic breast cancer cells in a stem-cell-like state, which enables them to propagate and form a new ... Breast cancer cells frequently move from their primary site and invade bone, decreasing a patients chance of survival. This ... Gomis and colleagues showed that once breast cancer cells are on the move NOG enables them to specifically invade the bone and ... First, NOG escalates bone degeneration by increasing the number of mature osteoclasts (bone cells that break down bone), ...
Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing ... Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions ... Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem ... Imaging and Tracking of Bone Marrow-Derived Immune and Stem Cells Youbo Zhao, Andrew J. Bower, Benedikt W. Graf, Marni D. ...
Decrying the ease with which law enforcement agencies may soon be able to track cellular phones and retrieve the contents of ... The rules require cell phone service providers to be able to pinpoint the physical locations of cell phones when they are in ... Equally disturbing is the requirement that cell phone service providers disclose where cell phones are physically located when ... "Everybody should be concerned by the notion that the state is taking on the ability to track its citizens," she said. The ...
Tenure Track Position T cell Immunology/Cancer Immunotherapy University of Hawaii at Manoa, with University of Hawaii Cancer ... Tenure Track Position T cell Immunology/Cancer Immunotherapy University of Hawaii at Manoa. Assistant Professor - Tenure Track ... Demonstrated track record of cancer immunotherapy T cell focused research. - Associate Professor level candidates should have ... Assistant Professor - Tenure Track Position T cell Immunology/Cancer Immunotherapy University of Hawaii at Manoa ...
Tracking the Fate of Iron-Labeled Cells: Differentiation Between Live and Dead Cells. Daniel Jirak1, Jan Kriz1, Ayman Oweida1, ... Quantitative Tracking of Magnetically Labeled Breast Cancer Cells in Rat Brain with a Fast T2 Mapping Technique. wei liu1, 2, ... Longitudinal MR tracking of mesioangioblasts was achieved following SPIO labeling of cells followed by high-resolution imaging. ... Cellular labelling with MRI contrast agents is becoming an established method of tracking cells in vivo, and it may also be ...
Live and fixed cell imaging demonstrated that mRNAs can extend beyond the protein surface of a stress granule, which may ... Herein, we used multicolour single-molecule tracking to quantify the precise timing and kinetics of single mRNAs as they exit ... Single-cell translation resumption during the recovery from arsenite stress (representative cell shown from 4 cells collected ... A representative long-term interaction between a translating KDM5B mRNA and a SG (,2 min) from 2 mRNAs tracked from 2 cells ...
... by Francis Tan - in Asia ... proposes to track each individual citizens movement in real time via cell phone signals, as reported on the Beijing Municipal ... Using this data, combined with dynamic analysis, the staff will be able to target specific areas at different times and track ... Li Guoguang, Deputy Director of Social Development believes that cellular location technology for real-time user tracking is an ...
... did not have a reasonable expectation of privacy in the data emanating from his cell phone that showed its location.... ... No warrant was required to track a drug dealers GPS locations from cell phones since the defendant ... Peter S. Vogel: Warrantless Tracking of Cell GPS Upheld by 6th Circuit. By Peter S. Vogel ... No warrant was required to track a drug dealers GPS locations from cell phones since the defendant did not have a reasonable ...
A ruling this week in a United States appeals court means officers of the law can legally and physically track down suspects ... based off of cell phone data without ever obtaining a warrant. ... Federal court approves warrantless tracking of cell phone users ... A cell subscriber, said the appeals court, "like a telephone user, understands that his cellphone must send a signal to a ... "We understand the cell phone users may reasonably want their location information to remain private," wrote the court. "But the ...
... provides a comprehensive overview of cell therapy ... Magnetic Resonance Imaging Cell-Labeling Methods. Ultrasound Cell-Labeling Methods. X-Ray-Guided Delivery and Tracking of Cells ... Other Non-Stem Cell Therapies for Cellular Tracking-Inflammatory Cell Tracking. Bioluminescence, MRI, and PET Imaging ... Human Embryonic Stem Cells. Mesenchymal Stem Cells from Bone Marrow. Hematopoietic Stem Cells. Adipose-Derived Adult Stem Cells ...
Technology and Law held a hearing last week to consider the privacy implications of cell phone tracking. Executives from both ... Apple reiterated that its cell phone tracking does not collect locations, but instead tracks nearby cell towers and hotspots so ... Unfortunately, the cell phone tracking debate is far from over.. Also chiming in on the issue are representatives from the ... Until regulatory officials decide how to proceed, the only recourse for users affected by cell phone tracking is to file ...
... motor proteins called kinesins work as transporters that haul materials built in one part of the cell to the place where they ... Keeping track of cell shapes and functions Taken together, this new research on the regulators of motor proteins and ... Keeping plant-cell motors on track Molecular brakemen keep transporter proteins in check until its their turn to move ... A rigid cell wall is an essential and energetically expensive investment for a plant. The cell wall confers strength and ...
... so-called dormant leukemia cells. The research results have now been published in the Cancer Cell journal. ... from Helmholtz Zentrum München and her colleagues have succeeded in finding a small population of inactive leukemia cells that ... the scientists isolated cells and identified a rare cell type that resembled cells triggering relapse. These cells were ... Isolated cells respond to drugs. "We have found a method to dissociate dormant leukaemia cells from their surroundings, where ...
Finally, we describe the different methods available and frequently used to track and identify the injected cells ,i,in vivo,/i ... and MSC-like cells and factors influencing this. We also discuss studies related to the mechanism of migration and homing and ... we discuss the migration and homing ability of mesenchymal stem cells (MSCs) ... To trace cells by MRI requires labeling of the cells with contrast reagents in order for the cells to be visualized. Cells can ...
This technology provides more tracking data at a faster rate and lower cost than traditional satellite tracking systems. The ... These wildlife tracking datasets are crucial for understanding species ranges, seasonal migration patterns, habitat use, ... The Information Science Branch explored and developed ways to track species locations using a standard cellular phone network. ...
Police say Stingray, a suitcase-size device that pretends its a cell tower, is useful for catching criminals, but thats about ... Police nationwide keep quiet about cell-tracking technology. By Jack Gillum, Associated Press; Published 9:09 a.m. CT March 22 ... Police nationwide keep quiet about cell-tracking technology. Police across the country may be intercepting phone calls or text ... In a response to a records request from the Tallahassee Democrat newspaper about Floridas use of cell-tracking technology, the ...
Live-cell imaging experiments carried out to this end often produce massive amounts of time-lapse image data containing far ... Methods for cell and particle tracking Methods Enzymol. 2012;504:183-200. doi: 10.1016/B978-0-12-391857-4.00009-4. ... A recurring task in many experiments is the tracking of large numbers of cells or particles and the analysis of their (morpho) ... and quantitative measures for tracking and motion analysis of cells and particles in time-lapse microscopy images. ...
... enforcement and prosecutors would be crippled if they were required to obtain a search warrant for cell phone location tracking ... In fact, as the EFF put it, the DOJ thinks "any privacy protection is too much privacy protection for cell phone tracking." As ... DOJ Says Requiring Warrant for Cell Phone Tracking Would Cripple Law Enforcement. * ... This story, "DOJ Says Requiring Warrant for Cell Phone Tracking Would Cripple Law Enforcement" was originally published by ...
Korea In Vivo Tracking of Mesenchymal Stem Cel… ... In Vivo Tracking of Mesenchymal Stem Cells using Fluorescent ... In Vivo Tracking of Mesenchymal Stem Cells using Fluorescent Nanoparticles in... * 1. Jong Min Lee, Jung Min Ahn, Gun-Il Im* ... Korea In Vivo Tracking of Mesenchymal Stem Cells using Fluorescent Nanoparticles in an Osteochondral Repair Model Provided by: ... 4. Cartilage Regeneration without Cell Implantation M M M M Application of In Vivo Growth Factors- Control Releasing System ( ...
... idea that best way to repair body is with its own system of cells and signaling molecules; difficulties of heart research do ... Four years after study in which doctors found that stem cells from bone marrow injected into damaged hearts of mice could morph ... into cells that body cannot replace after heart attack, treatment has yet to demonstrate whether it will fulfill its promise; ... not dash hopes for regenerative medicine, but simply suggest that stem cell treatments may require years to come to fruition; ...
... cell-site, or "ping" location data for mobile phones, according to a recent decision by the Sixth Circuit. ... Sixth Circuit okays warrantless tracking of cell phones Steptoe & Johnson LLP To view this article you need a PDF viewer such ... Police do not need a warrant to obtain GPS, cell-site, or "ping" location data for mobile phones, according to a recent ... "did not have a reasonable expectation of privacy in the data emanating from his cell phone that showed its location." ...
Tumor cells that begin to stray from the main tumor are followed by a string of stem cells that seem to be tracking and ... Noble, M. Can neural stem cells be used to track down and destroy migratory brain tumor cells while also providing a means of ... The ideal stem cell would first kill the tumor cell and then differentiate into normal brain cells repairing tumor damage and ... stem cells injected in or near the tumor would track and destroy tumor cells that have metastasized and left the main growth. ...
  • The advantage of the latter approach is that the label does not get lost during cell division and, thus, makes imaging of proliferating transplanted cells (e.g., tumor cells) possible. (
  • Research underway in San Diego analyzes blood from women with ovarian cancer to track tumor cells and better understand the spread of cancer. (
  • By analyzing circulating tumor cells in the blood stream they hope to get a better understanding of the spread of cancer. (
  • The authors of the new study suggest that specially prepared stem cells injected in or near the tumor would track and destroy tumor cells that have metastasized and left the main growth. (
  • The researchers can follow the activities of the tumor cells and the stem cells because each is labeled with a different color dye: tumor cells are a fluorescent green, whereas stem cells are red. (
  • Tumor cells that begin to stray from the main tumor are followed by a string of stem cells that seem to be tracking and surrounding these escapees, says Snyder. (
  • CD converts a benign, non-toxic drug called 5-fluorocytosine into a toxic compound that kills rapidly dividing tumor cells. (
  • Noble, M. Can neural stem cells be used to track down and destroy migratory brain tumor cells while also providing a means of repairing tumor-associated damage? (
  • and interactions of effector and regulatory cells with each other and with tumor cells. (
  • A drug that is already clinically available for the treatment of nausea and psychosis, called prochlorperazine (PCZ), inhibits the internalization of receptors on the surface of tumor cells, thereby increasing the ability of anticancer antibodies to bind to the receptors and mount more effective immune responses. (
  • In a disease such as cancer, that capability would provide a much better picture of what is going on in individual tumor cells. (
  • A team of researchers from UT Austin's Cockrell School of Engineering and College of Natural Sciences, including integrative biology postdoctoral researcher Kaitlyn Johnson, developed a new way to tag tumor cells to figure out how they evolve and change over time to resist cancer treatments. (
  • However, current capabilities don't paint a full picture of how tumor cells evolve. (
  • What this platform, known as ClonMapper, can do that wasn't possible before is look backward and trace how tumor cells change over time. (
  • Tumor cells adjust to treatments and become resistant. (
  • Stanger mentioned that the theoretical application of Barnea's research is simple: "We can use the same toolbox that was developed in Barnea's research and get tumor cells to mark all the cells that they are in touch with as they metastasize. (
  • Cell-based therapies may gain future importance in defeating different kinds of diseases, including cancer, immunological disorders, neurodegenerative diseases, cardiac infarction and stroke. (
  • Cell based therapies represent a challenge for noninvasive monitoring due to the variability in stem cell incorporation that occurs in the presence of massive cell death. (
  • Other Non-Stem Cell Therapies for Cellular Tracking-Inflammatory Cell Tracking. (
  • Now the way is paved for research into new therapies that prevent disease relapse by eliminating the remaining, so-called dormant leukemia cells. (
  • New therapies are therefore needed to eliminate these cells. (
  • Our new approach is to isolate dormant cells, which gives us the first possibility of developing therapies that switch off these cells. (
  • It might serve as basis for new therapies that destroy resistant leukaemia cells before they induce relapse. (
  • Our medium range goal is to develop new drugs, vaccines against EBV, and cell-based therapies in order to efficiently treat or prevent infectious diseases, leukaemia and lymphoma. (
  • Hence, their potential use in cell-based therapies will require that no undifferentiated ESCs persist in the graft. (
  • HSCs have then also been used for cell-based therapies especially in an allogeneic setting for more than a quarter of a century. (
  • For these cell therapies to succeed, you need imaging systems. (
  • Cell-based therapies have become a major focus in regenerative medicine and tumor treatment. (
  • Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. (
  • Knowing whether the stem cells made it to a target and have remained there long enough can go a long way in helping researchers study new stem cell therapies. (
  • In 2017, the Food and Drug Administration (FDA) approved tisagenlecleucel (Kymriah®) and axicabtagene ciloleucel (Yescarta®) CAR T-cell therapies for the treatment of certain relapsed or refractory leukemias and lymphomas. (
  • As more advanced stem cell therapies continue to be developed for all kinds of conditions, it remains a challenge to monitor what happens over time to the seeded cells. (
  • Cancer cells circulating in the blood carry newly identified proteins that could be screened to improve prognostic tests and suggest targets for therapies, report scientists at the Duke Cancer Institute. (
  • That initiative set aside $3 billion to help stem cell researchers develop therapies for various conditions. (
  • We know it appears to be more prevalent in cancer cells and now we can probe what role it is playing and potentially how to block it, potentially devising new therapies. (
  • PET reporter gene technology, multimodality reporter genes, imaging of gene/cell therapies, imaging of the immune system, imaging of intracellular events in living subjects (e.g., protein-protein interactions), bioluminescence resonance energy transfer (BRET) in living subjects, nanoparticle based imaging, Raman imaging in vivo and photoacoustic molecular imaging with novel imaging agents in living subjects. (
  • Here, cells are labeled either with fluorescent dyes or quantum dots, or transfected with plasmids coding for fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP). (
  • In Vivo Tracking of Mesenchymal Stem Cells using Fluorescent Nanoparticles in. (
  • Cell movement and location studies require specialized probes that are nontoxic to living cells and are available in a range of fluorescent colors to match instrument lasers and filters and to accommodate co-staining with antibodies or other cell analysis probes. (
  • Choose passively loaded dyes for short- and long-term tracking or fluorescent fusion proteins for cell tracking over 5 or more days. (
  • Invitrogen CellTracker dyes exhibit ideal properties-they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. (
  • Transiently transduced cells express fluorescent fusion proteins at different locations within the cell for at least 5 days or up to 2 weeks in slow-growing populations. (
  • Labeled cells are easy to track, but not all cells within a population will express the fluorescent protein. (
  • Invitrogen CellLight fluorescent proteins can be expressed in a variety of cell types including numerous transformed cell lines, primary cells, stem cells and neurons. (
  • Porcine primary skeletal muscle cell transduced with CellLight Nucleus-GFP fluorescent proteins. (
  • We demonstrate unique labeling of cells, driven by the heterogeneous random uptake of fluorescent nanoparticles of different emission colors. (
  • Invitrogen™ Molecular Probes™ fluorescent products provide a unique toolbox for studies of cell proliferation, migration, chemotaxis, and invasion. (
  • Labeling strategies range from expressing fluorescent proteins to loading long-lasting probes or cell-permeant cytoplasmic labels to fixable membrane tracers. (
  • Find live-cell probes for short-term tracking (3-6 generations), long-term tracking (6-10 generations), and fluorescent protein-based cell tracking solutions. (
  • Find fluorescent tracers for dissecting neural networks and their functions, including probes for fresh tissue sections, cultured cells, fixed tissues, and in vivo imaging. (
  • For flow cytometry applications, we offer Molecular Probes fluorescent stains and probes for short- and long-term tracing in fixable and nonfixable cell samples. (
  • Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. (
  • Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. (
  • For tracking single particles organic dyes and fluorescent proteins are usually used for labeling mRNAs. (
  • Their optical properties potentially make them important tools for biomedical imaging applications, as their fluorescent glow can be used to highlight living cells or cellular structures of interest under the microscope. (
  • There is also fluorescence microscopy, where fluorescent proteins or dyes are placed in the cells or in parts of the cells, so that they emit light of a specific wavelength when they are illuminated with light of a different wavelength. (
  • The lateral mobility of individual murine polyoma virus-like particles (VLPs) bound to live cells and artificial lipid bilayers was studied by single fluorescent particle tracking using total internal reflection fluorescence microscopy. (
  • Dynamic events at the plasma membrane of live cells are commonly studied with light microscopy and single-particle tracking (SPT) after labeling of specific membrane components with individual fluorophores, colloidal gold, or fluorescent beads ( 1 - 3 ). (
  • We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. (
  • A research team from the University of Leeds, Imperial College London and the University of Cambridge has invented a fluorescent marker that is able to attach to DNA G-quadruplexes in living human cells, allowing them to see for the first time how the structure forms and what role it plays in cells. (
  • The exceptional cellular uptake and intracellular boron release, together with the fluorescent and biocompatibility properties, make compounds 7 and 8 good candidates for in vitro cell tracking. (
  • The most common type of particles used in single particle tracking are based either on scatterers, such as polystyrene beads or gold nanoparticles that can be tracked using bright field illumination, or fluorescent particles. (
  • Billotey C, Wilhelm C, Devaud M et al (2003) Cell internalization of anionic maghemite nanoparticles: quantitative effect on magnetic resonance imaging. (
  • Still, he was encouraged by the finding that the nanoparticles did not affect stem cell differentiation or division. (
  • Scientists "labeled" the cells with superparamagnetic iron oxide nanoparticles (SPIONs) before they were administered to the patients. (
  • Researchers at the University of Liverpool have developed a photothermal microscopy technique for tracking a common stem cell marker, superparamagnetic iron oxide nanoparticles (SPIONs), over an extended period of time. (
  • Nanometer-sized magnetic particles (aka "nanoparticles") have been developed to track transplanted cells and have shown great success. (
  • The nanoparticles can be inserted into stem cells in the lab. (
  • The magnetic nanoparticles are mostly beneficial for tracking the location of cells, but are limited in providing additional information, such as whether the cells are dead or alive or actually performing the required function. (
  • In summary, nanoparticles enable the non-invasive tracking of transplanted cells, and now, the new technology of reporter genes will allow us to monitor cellular viability and function as well. (
  • A Stanford University School of Medicine team led by Cathy Shachaf, Ph.D., has for the first time used specially designed dye-containing nanoparticles to simultaneously image two features within single cells. (
  • With contributions from some of the most prominent preclinical and clinical researchers in the field, the book helps readers to understand the evolving concepts of stem cell labeling and tracking as the field continues to move forward. (
  • With a handle on IMB4, researchers now have a better understanding of the mechanical workings of the engines of cell wall deposition. (
  • In April 2001, researchers from the New York Medical College and the National Institutes of Health announced electrifying news for heart surgeons and their patients: stem cells from bone marrow, injected into the damaged hearts of mice, had morphed into the special cardiac muscle cells that the body cannot replace after a heart attack. (
  • To test the migratory potential of the stem cells, researchers created a population of mice with tumors in the right frontal lobe of the brain. (
  • Now, researchers at the Stanford University School of Medicine in California have developed a novel nanoparticle-based system that allows stem cells to be tracked in real time in a living mouse for up to a year after injection. (
  • After injecting bone marrow stem cells labeled with these particles into the hearts of healthy mice, the researchers found that they could track the cells using a standard ultrasound device. (
  • Nanoparticle imaging also demonstrated remarkable sensitivity, as the researchers were able to visualize as few as 75,000 stem cells with ultrasound, and a few hundred thousand with MRI. (
  • The researchers first plan to test the system in larger animal studies with other populations of stem cells. (
  • Researchers currently track whether cells reach their destinations using radioisotopes or by implanting a "reporter" gene. (
  • In recent years, researchers had developed ways to label cells for tracking by MRI. (
  • By using MRI to track how cells travel or home in to a desired site, researchers will be able to compare dosage amounts and frequency for the most effective treatments. (
  • Researchers at Rice and the Texas Heart Institute at St. Luke's Episcopal Hospital in Houston reported in the journal Biomaterials that mesenchymal stem cells drawn from pig bone marrow labeled with GNTs are easily spotted under MRI. (
  • Australian researchers have developed a technique for spotting rare immune cells that are reactive against cancer cells from within a patient's own immune system. (
  • Previous approaches have been able to read the long stretches of genetic output (the RNA) that encodes an immune cell's receptor from single cells, but have not, the researchers say, succeeded in sorting through the thousands of cells present in a tumour at a single time. (
  • In a proof-of-principle study, the researchers used the method to sample 7138 cells from the tumour and associated lymph node of a breast cancer patient. (
  • Led by Neal Halsey , MD, principal investigator and a professor with the Johns Hopkins Bloomberg School of Public Health's Department of International Health , along with researchers from Interactive Research & Development (IRD) and the Indus Hospital in Karachi, Pakistan, the program is a real-time patient tracking and referral system designed for use in low-resource settings. (
  • Single bacterial cells grow in about 2000 channels of a thousandth of a millimeter in diameter and can be individually studied in detail by the researchers in Prof. Erik van Nimwegen's group at the Biozentrum, University of Basel. (
  • Using the new system the researchers can now study precisely how genes are regulated in single cells under changing environmental conditions. (
  • Researchers at the University of Iowa did just that, documenting in real time and in 3-D how melanoma cells form tumors (See videos). (
  • In experiments with mice, researchers at the Johns Hopkins Kimmel Cancer Center say they have slowed the growth of transplanted human prostate and pancreatic cancer cells by introducing bone marrow cells with a specific gene deletion to induce a novel immune response. (
  • Following the first US test of CRISPR gene editing in patients with advanced cancer, researchers report these patients experienced no negative side effects and that the engineered T cells persisted in their bodies -- for months. (
  • NEW YORK (GenomeWeb) - Massachusetts Institute of Technology researchers have developed a microfluidic platform allowing them to trace cells as they divide. (
  • Using the platform, the researchers were also able to remove the daughter cells for single-cell analysis, as MIT's Scott Manalis and his colleagues reported today in Nature Communications . (
  • Working towards that, researchers at Rice University managed to insert bits of a bismuth based compound inside of ultra-short carbon nanotubes and the resulting material showed very high contrast under CT imaging when looking at pig bone marrow-derived mesenchymal stem cells (MSCs). (
  • The researchers believe the new material will help advance stem cell research and possibly allow for a way to track developing cells right in the patient's body. (
  • According to a study by Japanese researchers, the SMILE combination chemotherapy protocol is effective against extranodal natural killer/T-cell. (
  • Researchers think that for cancer to develop, damaged cells have to undergo certain transitions that cause them to spread, or metastasize. (
  • Cancer researchers adopting that philosophy have developed a new imaging technology that could give scientists the ability to simultaneously measure as many as 100 or more distinct features in or on a single cell. (
  • Researchers have developed a new way to observe and track large number. (
  • Researchers have developed a new way to observe and track large numbers of rapidly moving objects under a microscope, capturing precise motion paths in three dimensions. (
  • Over the course of the study--reported online Sept. 17, 2012, in the Proceedings of the National Academy of Sciences --researchers followed an unprecedented 24,000 rapidly moving cells over wide fields of view and through large sample volumes, recording each cell's path for as long as 20 seconds. (
  • The researchers tracked several cohorts of more than 1,500 human male gamete cells over a relatively wide field of view (more than 17 square millimeters) and large sample volume (up to 17 cubic millimeters) over several seconds. (
  • The researchers found that human male gamete cells travel in a series of twists and turns along a constantly changing path that occasionally follows a tight helix - a spiral that, 90 percent of the time, is in a clockwise (right-handed) direction. (
  • Because only four to five percent of the cells in a given sample traveled in a helical path at any given time, researchers would not have been able to observe the rare behavior without the new high-throughput microscopy technique. (
  • Now, a tiny chip, created through a collaboration of U-M Comprehensive Cancer Center and Department of Electrical Engineering and Computer Science researchers, makes it possible to isolate and study individual metastatic cells. (
  • With the speedy cells isolated and their secrets revealed, future researchers could try targeted drugs on them before treating a patient with a regimen tailored to that patient's particular metastatic cells. (
  • HeLa cells, a cancerous cell line used by researchers around the world to study a large variety of important research questions. (
  • That gives researchers the ability to look at which cells "win out" over less resistant cells, continue to clone themselves and make the tumor more dangerous. (
  • By isolating these cells, researchers can better test which treatments do and don't work against them. (
  • In a first of its kind study, researchers at Johns Hopkins Medicine have developed a new technique that transports therapeutic stem cells in a multilayer microcapsule that not only protects the cells from being attacked by the body's immune system but also enables them to be seen on X-ray. (
  • In this chapter, different ways to label and track cells in vivo are described. (
  • Ahrens ET, Feili-Hariri M, Xu H et al (2003) Receptor-mediated endocytosis of iron-oxide particles provides efficient labeling of dendritic cells for in vivo MR imaging. (
  • Ahrens ET, Flores R, Xu H et al (2005) In vivo imaging platform for tracking immunotherapeutic cells. (
  • Cellular imaging using magnetodendrimers: application to human stem cells and neoplastic cells in vivo. (
  • Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. (
  • Finally, we describe the different methods available and frequently used to track and identify the injected cells in vivo . (
  • Apart from being able to differentiate to multiple cell types in vitro (and in vivo ), MSCs and MSC-like adult stem cells have extensive immunomodulatory and immunological tolerance inducing characteristics [ 20 - 26 ]. (
  • In vivo MRI cell tracking using perfluorocarbon probes and fluorine-19 detection. (
  • This article presents a brief review of preclinical in vivo cell-tracking methods and applications using perfluorocarbon (PFC) probes and fluorine-19 ((19) F) MRI detection. (
  • We discuss the compositions of matter, methods and applications of PFC-based cell tracking using ex vivo and in situ PFC labeling in preclinical studies of inflammation and cellular therapeutics. (
  • In vivo cytometry examples using ex vivo PFC labeled cells in rodent models. (
  • a ) Shows CD4+ T cells from a wild-type DBA mouse, labeled ex vivo , and infused back into a recipient of the same strain. (
  • The in vivo 19 F image (pseudo-color) through the torso shows a localized accumulation of T cells in periaortic lymph nodes. (
  • Ultimately, the team hopes the magnetic properties of tagged stem cells will allow doctors to manipulate them in vivo and direct cells to specific locations -- in the heart, for instance -- where they can heal damaged tissue. (
  • Cell Viability and Noninvasive In Vivo MRI Tracking of 3D Cell En. (
  • We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of cell encapsulation. (
  • Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue ® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune cell functions in vitro and in vivo . (
  • Our findings suggest that the [email protected] can be used as a potential new class of X-ray CT agent for stem cell labeling and possibly in vivo tracking. (
  • Arbab AS, Pandit SD, Anderson SA et al (2006) MRI and confocal microscopy studies of magnetically labeled endothelial progenitor cells trafficking to sites of tumor angiogenesis. (
  • The book first presents background information and insight on the major classes of stem and progenitor cells. (
  • Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche. (
  • While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. (
  • We validate the proposed pipeline by tracking pig neural progenitor cells through a time lapse experiment consisting of 825 images collected over 69 hours. (
  • Mesenchymal Stem Cells from Bone Marrow. (
  • In this review, we discuss the migration and homing ability of mesenchymal stem cells (MSCs) and MSC-like cells and factors influencing this. (
  • Later on, Caplan and others termed these cells "mesenchymal stem cells" (MSCs) [ 3 , 4 ]. (
  • Labeling and tracking of mesenchymal stromal cells with EdU. (
  • In the present study we tested a new thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), for labeling and tracking of mesenchymal stromal cells (MSC), specifically adipose tissue-derived stem cells (ADSC). (
  • Caption: Dark spots are aggregates of gadonanotubes (GNTs) in the cytoplasm of a mesenchymal stem cell. (
  • An X-ray image of unlabeled mesenchymal stem cells in test tubes shows the dramatic difference between those tagged with nanotubes that don't include bismuth (left) and those that do (right). (
  • Mesenchymal cells are more motile, while epithelial cells tend to be fixed in rows. (
  • The hypothesis is that when epithelial cells form tumors, like in colon, prostate or breast cancer , the cells at the edge of the tumor have to turn to mesenchymal cells for the cancer metastasize. (
  • Then, "the mesenchymal cells can leave the tumor, get into the blood stream and spread around your body," Bepler said. (
  • Within both plant and animal cells, motor proteins act like the engines in a busy train system. (
  • In a growing plant cell, motor proteins called kinesins work as transporters that haul materials built in one part of the cell to the place where they are needed. (
  • But further down the stem, where the cells had ceased to elongate, the motor proteins were gone. (
  • For example, antibodies against cell lineage markers, such as CD2, CD19, T cell receptors, and Ig proteins, are not available, making the identification of zebrafish T and B cell populations difficult. (
  • In cells, metabolic activity generates chemical nonequilibrium, which powers mechanoenzymes (motor proteins). (
  • Study shows Colorado drug SVC112 stops production of proteins that cancer stem cells need to survive and grow. (
  • Imaging and tracking RNA molecules also allow studying RNA molecules not translated into proteins. (
  • The entry of animal viruses into their host cells starts with binding of the particles to lipids, proteins, or carbohydrates that serve as receptors on the cell surface. (
  • Cancer starts out in a single cell, and its development is often heralded by changes in the activation levels of certain proteins. (
  • In the world of cell biology , one common way for proteins to become activated is through a process called phosphorylation, which slightly changes a protein's shape, in effect, turning it on. (
  • The Stanford team was able to simultaneously monitor changes in the phosphorylation levels of both proteins in lab-cultured myeloid leukemia cells. (
  • Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention. (
  • In life sciences, single-particle tracking is broadly used to quantify the dynamics of molecules/proteins in live cells (of bacteria, yeast, mammalian cells and live Drosophila embryos). (
  • Here we present data from cellular MRI experiments in various models which suggests that whether or not the signal loss remains over time, when cells die, depends on the host tissue. (
  • Through clear descriptions and color images, this volume illustrates how noninvasive imaging is used to track stem cells as they repair damaged tissue in the body. (
  • Although embryonic stem cells (ESCs) are pluripotent and could be used to replace any tissue, they can form teratomas. (
  • Although the cell surface repertoire and the gene expression pattern vary among these cells, this is likely a reflection of the tissue of origin or the culture conditions used for maintenance of these cells [ 11 , 12 ]. (
  • The finding holds particularly interesting possibilities for malignant brain cancers whose cells aggressively spread throughout normal brain tissue seeding new tumors, a characteristic that makes treatment almost impossible. (
  • Stem cells injected close to the tumor migrated through normal healthy adult brain tissue to infiltrate the tumor. (
  • He also suggests that further work is needed to prove that stem cells can hunt down an individual tumor cell that infiltrates healthy tissue. (
  • Transplanted cells used to replace damaged and degenerated tissue. (
  • Stem cells hold enormous potential for repairing or regenerating damaged tissue . (
  • BrdU) has been used widely to label cells in culture and in tissue. (
  • Long-term (2-6 week) follow-ups found EdU-positive cells only in the connective tissue. (
  • By "scanning" the relevant immune cell receptors in many thousands of cells at once, RAGE-seq can provide an accurate snapshot of how the immune cells in a tissue sample are related, and which cells may be effective at mounting a response against cancer. (
  • Studies have already shown that if cells migrate to the circulatory system, beyond their target organ or tissue site, then it can cause inflammation in the body," said Dr Lara Bogart, scientist at the University's Institute of Integrative Biology in a statement. (
  • Stem cells are used to treat conditions such as leukaemia and have the potential to treat many more diseases and disorders where patient survival is reliant on organ and tissue donation. (
  • Optogenetics is a new biological technique that uses light to control cells, particularly neurons, in living tissue can be used create a powerful new tool for studying brain activity. (
  • Based on their research on bioluminescence in "a scummy little organism, the green alga Chlamydomonas, that nobody cares much about" Johnson and his colleagues realized that if they could combine luminescence with optogenetics -- a new biological technique that uses light to control cells, particularly neurons, in living tissue -- they could create a powerful new tool for studying brain activity. (
  • Biology professor David Soll and his team used unique computer-assisted 3-D reconstruction software to chronicle how both breast tissue cancer cells and melanoma cells form tumors. (
  • Still, how melanoma cells join into tumors--whether by individual cells coming together or small or large clusters of cells doing so--follows the same pattern as breast tissue cancer cells: Cables are extended to reel in other cells or clusters. (
  • A systematic examination of the tumor and the tissue surrounding it -- particularly normal cells in that tissue, called fibroblasts -- has revealed a new treatment target that could potentially prevent the rapid dissemination and poor prognosis associated with high-grade serous carcinoma (HGSC), a tumor type that primarily originates in the fallopian tubes or ovaries and spreads throughout the abdominal cavity. (
  • Stem cells have the capability to induce tissue repair and ultimately reverse the progression of many diseases. (
  • Stem cells have the ability to migrate into the damaged tissue, replace the non-functioning cells and take over their function. (
  • Stem cells secrete small molecules that can prevent cell/tissue death, and help in recovery, or at least prevent deterioration of the tissue. (
  • Here, trajectories of individual, fluorescence-labeled VLPs on the surface of tissue culture cells and in artificial lipid bilayers were recorded with total internal reflection fluorescence (TIRF) microscopy for SPT. (
  • Noninvasive imaging of fewer or even single cells succeeds with MRI after labeling of the cells with (ultrasmall) superparamagentic iron oxide particles (SPIO and USPIO). (
  • Longitudinal MR tracking of mesioangioblasts was achieved following SPIO labeling of cells followed by high-resolution imaging. (
  • Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging provides a comprehensive overview of cell therapy imaging, ranging from the basic biology of cell therapeutic choices to the preclinical and clinical applications of cell therapy. (
  • Introduction: Stem Cell Types Overview and Rationale for Labeling for Imaging. (
  • Radionuclide Cell-Labeling Methods. (
  • Magnetic Resonance Imaging Cell-Labeling Methods. (
  • Cell labeling with EdU in culture or living animals can be performed easily. (
  • U2-OS cells were transduced with CellLight Plasma Membrane-GFP (green), followed by labeling with Qtracker 655 Cell Labeling Kit (magenta), and HCS NuclearMask Blue (blue). (
  • These diverse reagent classes can be used for labeling mammalian cells to view changes in morphology and location. (
  • The labeling protocol is rapid, simple, and applicable to essentially any membrane-containing cell or particle ( Figure S2 *), but obtaining bright, uniform, and reproducible labeling requires attention to different variables than when staining with antibodies or other equilibrium binding reagents 1-3 . (
  • The amount of dye required for bright, homogeneous labeling increases as total number and/or size of cells to be stained increases (i.e., as total amount of membrane increases). (
  • In our experience, a preliminary titration at constant cell concentration is the easiest way to select an optimized dye concentration that gives acceptable post-labeling viability, recovery, and fluorescence intensity as well as unaltered cell function 2,3 . (
  • Uniform labeling requires rapid, homogeneous mixing of cells and dye. (
  • In theory, it should make no difference whether cells are added to dye or dye to cells, but in our experience new users can more reliably obtain uniform labeling by adding cells to dye 3 . (
  • Diluent C is an aqueous staining vehicle designed to keep cells viable and membrane dyes in solution for long enough to complete the brief labeling step. (
  • Since labeling occurs by partitioning rather than equilibrium binding, dye uptake into membrane continues for as long as cells are in contact with dye, eventually compromising membrane integrity and function. (
  • To check whether metabolite tracking was possible by fluorescence labeling, the scientists attached fluorophores either to ceramide, which is a member of the sphingolipid class, glucose, or lactic acid. (
  • It can be used at concentrations ranging from 1 - 20 µM for cell labeling. (
  • Today the Supreme Court announced it will review United States v. Carpenter , a case involving long-term, retrospective tracking of a person's movements using information generated by his cell phone. (
  • There's no question that cell phones and cell-phone records can be useful for police officers who need to track the movements of those they believe to be breaking the law," the group added. (
  • When a cholera outbreak began months after the January 2010 earthquake in Haiti, health workers used cell phones to help track the movements of people leaving the epicenter, allowing them "to alert medics to go where infected people might carry the disease," according to a report published on Tuesday in PLoS Medicine, NPR's health blog " Shots " reports. (
  • Apart from parents who want to track the movements of their children, the software also helps corporates to track their on-field employees. (
  • The bill would require law enforcement to show probable cause and get a warrant before acquiring geolocational information and create criminal penalties for secretly using an electronic device to track a person's movements. (
  • It's also a very efficient tracking beacon for following your movements from place to place. (
  • On November 23, the Washington Post reported that federal officials regularly obtain cell phone location data from phone companies so the government can track individuals movements. (
  • According to the Post, federal officials sometimes do so without any court supervision, and sometimes act in violation of a DOJ recommendation that federal officials establish probable cause before tracking the movements of individuals in private spaces. (
  • It's obviously not because they think it would be creepy to track the movements of every cell phone user in the country. (
  • From Black Friday through New Year's Day, the mall will use your phone to track your movements. (
  • There are many reasons you might want to locate a cell phone and track its movements. (
  • Labelling stem cells is hugely valuable to tracking their movements in the body, but we need to know more about how the particles used interact with stem cells. (
  • movements by monitoring the signals from their cell phones. (
  • But the reality is, what happens when you start having hackers potentially having access to this information and being able to track your movements? (
  • Breast cancer cells, in contrast, are more ponderous and lumbering in both their movements and in forming tumors. (
  • Yet one could secretly install allowing girlfriends, boyfriends, husbands and wives to track each others' movements. (
  • The team of scientists headed by Jeremias, head of the 'Apoptosis' research group in the Gene Vectors Research Unit (AGV) at the Helmholtz Zentrum München, has now isolated and characterized therapy-resistant cells for the first time. (
  • With the help of modern genetic engineering* and dyes that mark cell growth, the scientists isolated cells and identified a rare cell type that resembled cells triggering relapse. (
  • This work, if replicated in humans, could finally allow scientists to verify if these cells are going where they're intended. (
  • The scientists detected no ill effects in several different types of cells that they tested. (
  • In the decades since the Till and McCulloch discovery, stem cells have come to be seen by scientists as potentially offering ways to treat - and perhaps cure - a host of devastating and costly illnesses such as cardiovascular disease, cancer, diabetes, blindness and neurodegenerative disorders. (
  • Scientists from the University of Liverpool have developed new methods to track stem cells and further understanding of what happens to them after they have been in the body for a significant period of time. (
  • Well, now scientists had developed a method to track the stem cells in our body, according to a new report. (
  • The magnetic resonance imaging (MRI) scans clearly showed movement of the stem cells and the scientists could determine whether the stem cells reached their intended target or not. (
  • However, scientists warn that conditions within the body's cells can lead to the degradation of SPIONs and reduce the ability of MRI scans to pick up on their signal in the long-term. (
  • To overcome this drawback, scientists are developing new methods to visualise SPION's in the cells before they enter the body to learn their performance in the long-term. (
  • The scientists created the technique as a new and improved method for tracking the interactions within large neural networks in the brain. (
  • The software was developed in collaboration with scientists from Prof. Gene Myers' research group at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden. (
  • To address the need for visualization of transplanted cells, including but not limited to stem cells, scientists started in the early 90's to develop new technologies. (
  • A team of Korean scientists headed by Bu Young Choi at Seowon University have described an elegant solution to this challenge by placing an xCELLigence Real-Time Cell Analyzer (RTCA) directly within a hypoxia chamber. (
  • Retrieved on July 12, 2020 from (
  • Junior Tristan Bepler, a biology and computer science major, is testing this hypothesis, studying two types of cell transitions scientists have linked to the spread of cancer. (
  • Successful development of the new technique may improve scientists' ability not only to diagnose cancers-for example, by determining how aggressive a tumor's constituent cells are-but also eventually to separate living, biopsied cancer cells from one another based on characteristics indicating their stage of progression or their degree of resistance to chemotherapeutic drugs. (
  • Although the new technology so far has been used only to view cells on slides, it could eventually be used in a manner similar to flow cytometry, the current state-of-the-art technology, which lets scientists visualize single cells in motion. (
  • T he formation of four-stranded DNA has been tracked in living human cells, allowing scientists to see how it works and its possible role in cancer. (
  • He said: Scientists need special probes to see molecules within living cells, however these probes can sometimes interact with the object we are trying to see. (
  • The two scientists aim to develop an effective way to track the movement of cancer cells and ultimately discover how and when tumors spread. (
  • Cell-tracking approaches using optical methods are important for preclinical research. (
  • Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny. (
  • In all of these incidents, Minnesota law-enforcement officials say the use of secretive cell-phone tracking devices were critical in quickly solving crimes that had stumped police using traditional methods. (
  • But these methods have limitations, including the short half-life of isotopes, dangers in modifying the cell genome and other complications. (
  • Other trackers and tracers in a wide variety of colors provide efficient and sensitive methods for monitoring specific cells within a population by flow cytometry or imaging, in culture or in whole animals. (
  • Previous culture methods didn't keep cells alive long enough to recapitulate this maturation process. (
  • We analyze the results using performance measures for segmentation and tracking that rank all participating methods. (
  • The changes in Stat1 and Stat6 closely tracked those demonstrated with existing visualization methods, establishing proof of principle for the new approach. (
  • Large scale in vitro cell growth experiments require automated segmentation and tracking methods to construct cell lineages in order to aid cell biologists in further analysis. (
  • Flexible segmentation algorithms that easily adapt to the specific type of problem at hand and directly applicable tracking methods are fundamental building blocks of setting up multi purpose pipelines. (
  • Funded by a grant from the Pew Charitable Trusts, Gilad Barnea, associate professor of neuroscience, will partner with Ben Stanger, associate professor of medicine at Penn, in an attempt to utilize specialized methods for tracking substances between nerve cells to study how cancer cells move. (
  • Bulte JWM, Lu J, Zywicke H et al (2001b) 3D MR tracking of magnetically labeled embryonic stem cells transplanted in the contusion injured rat spinal cord. (
  • Human Embryonic Stem Cells. (
  • Can the government use your cell phone records to track your physical location without first obtaining a warrant based on probable cause? (
  • Law enforcement officers in Washington, D.C. violated the Fourth Amendment when they used a cell site simulator to locate a suspect without a warrant, a D.C. appeals court ruled on Thursday. (
  • Washington, D.C.-The Electronic Frontier Foundation (EFF) urged the U.S. Supreme Court to review a troubling ruling that allows police to obtain-without a warrant-location data from people's cell phones to track them in real time. (
  • The ruling came in a public records lawsuit brought by the American Civil Liberties Union, which had requested information on federal cases in which law enforcement had obtained cellphone tracking data without a warrant to track a user's whereabouts. (
  • A federal appeals court on Wednesday rejected the Obama administration's contention that the government is never required to get a court warrant to obtain cell-site information that mobile-phone carriers retain on their customers. (
  • The Stored Communications Act , the appeals court ruled in September, granted judges the discretion to require a warrant under the Fourth Amendment for the government to obtain the cell-site information. (
  • No warrant was required to track a drug dealer's GPS locations from cell phones since the defendant 'did not have a reasonable expectation of privacy in the data emanating from his cell phone that showed its location. (
  • The Skinner case is different than the US v. Jones case decided earlier this year where the US Supreme Court ruled 9-0 that a GPS tracking device could not be attached to a suspect's vehicle without a warrant [ enhanced version ]. (
  • A ruling this week in a United States appeals court means officers of the law can legally and physically track down suspects based off of cell phone data without ever obtaining a warrant. (
  • The verdict overturns a ruling made in 2011 by a magistrate judge from Houston, Texas who said federal authorities weren't able to compel telecoms for 60 days' worth of cell phone records without a warrant. (
  • Both Montana and New Jersey approved legislation in June and July, respectively, saying a search warrant is required by state investigators in order to collect cell phone location data. (
  • An Obama administration official told a congressional panel that requiring a search warrant to obtain cell phone location tracking information would "cripple" law enforcement and prosecutors. (
  • Now that the Supreme Court decided a warrant is necessary before attaching a GPS to a person's vehicle, warrantless location tracking via a person's cell phone is the surveillance method of choice for law enforcement. (
  • But the Fourth Amendment grants us a "reasonable expectation of privacy" and the world as we know it didn't end when the Supreme Court ruled that GPS tracking required a warrant. (
  • Weinstein told the Congressional Internet Caucus Advisory Committee the DOJ tells law enforcement to obtain a search warrant when tracking a phone's GPS or "precise locational information," yet that requirement can vary. (
  • As for Weinstein's comment about the need for a warrant "crippling" prosecutors and law enforcement, if warrantless cell phone location tracking continues it will cripple the privacy of We the People. (
  • Police do not need a warrant to obtain GPS, cell-site, or "ping" location data for mobile phones, according to a recent decision by the Sixth Circuit. (
  • All it takes is for the phone to be turned on to work, and it allows police to track the cell phone without getting a warrant from a service or provider. (
  • The ACLU wants to know whether the 379 law enforcement agencies demonstrate probable cause and obtain a warrant to access cell phone location data, how frequently they obtain cell phone location data, the amount of money spent on tracking cell phones and other polices related to acquiring location data. (
  • On Tuesday we covered a disturbing story from the New York Times and detailing how some of the country's largest cellular providers have been selling your real-time location information, allowing a Texas-based prison technology company, Securus, to track any phone "within seconds" - all without a warrant - through an intermediary called LocationSmart. (
  • "Absent a search warrant," Judge Pauley stated in his 14-page opinion , the government may not turn a citizen's cell phone into a tracking device. (
  • The genetics underlying the development of the zebrafish lymphoid system is under active investigation, but functional studies of the zebrafish immune system have been limited by the lack of tools to facilitate the isolation and characterization of different lymphoid cell populations. (
  • Because of these limitations, studies in the zebrafish have relied on transgenic technology and on lineage-restricted gene promoters to drive the expression of GFP in specific blood cell populations ( 21 , 22 ). (
  • Using lck-GFP and rag2-GFP transgenic fish, we identify T and B cell populations in the zebrafish. (
  • Nanoparticle vesicle encoding for imaging and tracking cell populations. (
  • However, the inability to accurately identify, track and measure thousands of single cells via high-throughput microscopy has impeded dynamic studies of cell populations. (
  • Accurate detection of the live and dead bacteria in a sample is an important aspect of many experimental procedures in biotechnology, and due to the inherent differences between prokaryotic and eukaryotic cells, specialized dyes must be used to differentiate live and dead cell populations in bacteria. (
  • Within every malignant tumor, there can be thousands of genetically distinct cell populations. (
  • Ideal tracking dyes are passed to daughter cells for multiple generations but do not transfer to neighboring cells on contact. (
  • Table S1 * summarizes non-perturbing staining conditions reported for several membrane dyes, but concentrations found in the literature should always be tested on each laboratory's cell type(s) of interest. (
  • The strength of the fluorescence indicates the abundance of the cell-surface features to which those dyes are now attached. (
  • Whenever you use a cell phone. (
  • NEW YORK - If you are a U.S. resident who owns a cell phone, you should care about the outcome of a court case that "could well decide whether the government can use your cell phone to track you - even if it hasn't shown probable cause to believe it will turn up evidence of a crime. (
  • Back in 2007, the U.S. government applied for court permission to obtain information about the location of an individual's cell phone, without showing probable cause that tracking the individual would turn up evidence of a crime. (
  • A number of civil liberties groups, on behalf of plaintiffs in the case, filed a friend-of-the-court brief in support of the district court decision, arguing that district courts must require the government to show probable cause before permitting the government to obtain information about the location of a cell phone. (
  • Jersey and Delaware must show probable cause before tracking people's cell phone locations. (
  • The plaintiffs in the court case hope the court will "send a message that merely carrying a cell phone should not make people more susceptible to government surveillance. (
  • Is Your Cell Phone Accelerometer Tracking You? (
  • Your Phone Is Tracking You--Should You Care? (
  • Decrying the ease with which law enforcement agencies may soon be able to track cellular phones and retrieve the contents of cellular and land-line phone calls, three Internet privacy organizations are asking a federal appeals court to suspend rules that would make it unnecessary to obtain search warrants to intercept phone calls. (
  • The rules require cell phone service providers to be able to pinpoint the physical locations of cell phones when they are in use. (
  • Equally disturbing is the requirement that cell phone service providers disclose where cell phones are physically located when in use, said Shari Steele, director of legal services for the Electronic Frontier Foundation. (
  • Purportedly to improve Beijing's public travel and reduce traffic congestion, the new initiative, literally translated as "Platform for Citizen Movement Information" proposes to track each individual citizen's movement in real time via cell phone signals, as reported on the Beijing Municipal People's Government website. (
  • On Tuesday, justices there overturned Hughes' decision and said cell phone companies and their customers had no Fourth Amendment protected right to refute the government's request for information. (
  • We understand the cell phone users may reasonably want their location information to remain private ," wrote the court. (
  • In response to the news that both Apple and Google were recording the locations of smart phone users, the Senate Judiciary Subcommittee on Privacy, Technology and Law held a hearing last week to consider the privacy implications of cell phone tracking . (
  • Apple reiterated that its cell phone tracking does not collect locations, but instead tracks nearby cell towers and hotspots so that software can choose an appropriate ad. (
  • Unfortunately, the cell phone tracking debate is far from over. (
  • Until regulatory officials decide how to proceed, the only recourse for users affected by cell phone tracking is to file private lawsuits under invasion of privacy laws at both the state and federal levels. (
  • The Information Science Branch explored and developed ways to track species locations using a standard cellular phone network. (
  • Police nationwide keep quiet about cell-tracking technology Police across the country may be intercepting phone calls or text messages to find suspects using a technology tool known as Stingray. (
  • This Oct. 24, 2013 file photo shows a youth checking his smartphone in Glenview, Ill. Local police may be tracking your cell phone. (
  • If your mobile phone is on then it is constantly pinging cell phone network towers , leaving you no choice about revealing your location . (
  • The ACLU warned the "threat to personal privacy presented by this technology is breathtaking," especially since the "government is routinely violating American's privacy rights through warrantless cell phone tracking . (
  • Jason Weinstein , deputy assistant attorney general for the Justice Department's criminal division, discussed the need for such warrantless cell phone tracking so it won't "cripple" the government at " State of the Mobile Net ," a conference for the Congressional Internet Caucus Advisory Committee . (
  • selling cell phone surveillance records is a big money-making business for mobile phone companies that have special divisions and manuals to assist law enforcement in nabbing our info. (
  • In fact, as the EFF put it , the DOJ thinks "any privacy protection is too much privacy protection for cell phone tracking. (
  • In a 2-1 ruling, the court in United States v. Skinner held that the government's collection of these types of location data from a mobile carrier did not violate the suspect's Fourth Amendment rights because he "did not have a reasonable expectation of privacy in the data emanating from his cell phone that showed its location. (
  • Have there been attacks on the use of cell phone records in Canada on the ground that they are not reliable indicators of location? (
  • The US case referred to tracking by use of the relation of the phone to cell transmission towers, not to GPS data. (
  • Interesting that Senate officials used Duffy's cell phone records to find out where he was during claim periods. (
  • But lawmakers in Minnesota are taking a closer look at so-called "cell phone exploitation" devices - more commonly known by model names Kingfish and Stingray - after concerns have arisen regarding the ability to track the locations of a body of cell-phone devices at once. (
  • Cell-phone tracking devices, which are portable and can fit inside of a suitcase, work by impersonating a cell-phone tower and tricking a phone to connect to it using its own antenna. (
  • In most cases, law-enforcement agencies need a simple court order to use cell-phone tracking. (
  • Currently two law-enforcement agencies in Minnesota are known to use cell-phone-tracking devices: The Hennepin County Sheriff's Office has used the devices since 2010, and the Minnesota Bureau of Criminal Apprehension bought its first cell-phone tracker in 2005. (
  • So far, he hasn't learned of other agencies using cell-phone tracking in Minnesota. (
  • Cell-phone tracking is becoming a more popular method with police around the nation, used in at a least a dozen local law-enforcement agencies in states like Florida, Texas and California. (
  • Dohman said there have been no breaches of the data obtained by the cell-phone-tracking devices, and said the information is only "temporarily held" until it's overwritten by a new investigation. (
  • Dohman listed of a handful of examples, including abduction cases and the near sexual abuse of the 14-year-old girl, in which cell-phone tracking helped the Bureau of Criminal Apprehension solve crimes. (
  • Before GPS started being deployed in the majority of mobile devices sold on the market, the only method available to track a cell phone was triangulating the cell phone's position using one of several techniques available to law enforcement and other governmental agencies. (
  • Today, most cell phone tracking applications work by accessing the GPS location of the cell phone and providing feedback to the end-user in the form of an app or software program. (
  • In order to understand how cell phone tracking works, it becomes necessary to look at each major model of cell phone and the available options for locating the desired mobile phone. (
  • Some atmospheric facts can influence the accuracy of a GPS device, so when attempting to use a cell phone tracking application based on GPS, the locating data may be off as far as 50 feet from the exact location of the phone (a bigger deal for those who have misplaced a cell phone in their home as compared to trying to find out the geographic location of a phone). (
  • Before getting into detail on the various GPS tracking applications for cell phones, it's first worth reviewing some of the factors that can degrade the signal accuracy throwing off the location of the phone. (
  • Why is a mobile called a cell phone? (
  • Service provider Airtel provided a platform for the IIT trio to test their software, which was demonstrated at the recently concluded Mood Indigo festival on a sample crowd of 300 cell phone subscribers. (
  • American Civil Liberties Union (ACLU) affiliates in 34 states across the U.S. have filed public records requests seeking information from law enforcement agencies as to when, why and how they are using cell phone location data to track Americans. (
  • The ability to access cell phone location data is an incredibly powerful tool and its use is shrouded in secrecy. (
  • Carrying around a cell phone should not make a person susceptible to that kind of surveillance. (
  • It is unknown when the government began requesting orders for cell phone location data without first establishing probable cause or how many of the requested orders have been issued. (
  • The DOJ maintains that probable cause is not always necessary before acquiring individual cell phone locations. (
  • The request for information includes documents, memos and guides regarding the policies and procedures for tracking individuals through the use of cell phones, as well as information about the number of times the government has applied for cell phone location information without establishing probable cause and how many times it has been granted. (
  • RICHMOND, VA (WWBT) - If you're planning on doing your holiday shopping at Short Pump Town Center, you might consider turning off your cell phone. (
  • Short Pump will be using signals from every shopper's cell phone to track where he or she goes in the mall. (
  • In fact, we'll be tracking your movement from store to store through your cell phone. (
  • Whatever your reason for tracking a cell phone, there are several ways to go about doing it. (
  • Here's the scoop on cell phone tracking. (
  • Even if you're not a spy, you might have a perfectly legitimate need to track the location of a cell phone. (
  • Here's a roundup of tools to track a cell phone location. (
  • You have to download and install a Google App on your phone or computer to do this, and each person whose phone number you provide must agree to be tracked by you. (
  • The service can use GPS, wifi access points, or cell towers to determine the location of your phone. (
  • Google Latitude lets you control when your phone is tracked, so you can turn off permission to track you when you want to travel to parts unknown. (
  • If you have access to a GPS-enabled cell phone you can install software on it that enables tracking, sometimes without telling the user you've done so. (
  • AccuTracking is the only cell phone tracking system I know of that that will work with a regular cell phone (feature phone). (
  • The AccuTracking service lets you see the real-time location and speed of the phone being tracked, and will send email or text message alerts if that person moves outside of a designated boundary or exceeds the speed limit. (
  • Prey is a similar app that can help you track a phone, tablet or laptop. (
  • But GPS is not the only way to track a cell phone. (
  • But this type of cell phone tracking would require the cooperation of your service provider and law enforcement. (
  • Can you track a phone just by entering its number on a Web site? (
  • During each encounter, the doctor uses the cell phone to scan the child's ID tag, collecting pertinent immunization, clinical and laboratory data that can be viewed in real-time over a secure website. (
  • The demo, which has since been taken down, was a free service that would give anyone the approximate location of their own cell phones by entering their name, email address and phone number into a form. (
  • And the gist of it is I can track most peoples' cell phone without their consent. (
  • Xiao's tests showed that he could easily command LocationSmart's service to ping the closest cell phone tower to a subscriber's mobile device. (
  • He says he checked a friend's cell phone number multiple times over a few minutes while that friend was moving - and he was able to manually plug the provided coordinates into Google Maps to track his directional movement. (
  • Police tracking cell phones or searching a phone after making arrest has been a lightning rod topic in the justice system. (
  • The Supreme Court issued a pair of decisions in State v. Hinton and State v. Roden finding that police violated state law when, after seizing a cell phone from a suspect during a drug investigation, it monitored and responded to incoming text messages, arranging drug deals with defendants Hinton and Roden. (
  • To build a case against Mr. Lambis, DEA agents had used a 'Stingray' tracker to locate Lambis' cell phone that eventually was found by police inside Lambis' family home. (
  • While in a geographical area police can move the Stingray around and pick up cell phone calls, text messages, voice mail messages, and they are able to pinpoint the location of a targeted phone within a short distance. (
  • The tracking system, called FootPath Technology, works through a series of antennas positioned throughout the shopping center that capture the unique identification number assigned to each phone. (
  • We don't need to know who it is and we don't need to know anyone's cell phone number, nor do we want that,' Shriver-Engdahl said. (
  • Track Lost Cell Phone - Find Phone Location is a find phone and device locator tool app that efficiently track a cell phone. (
  • By using this find my phone and track my phone facility one can track a cell phone by simply sending SMS to misplaced/ find lost phone. (
  • If you are annoyed to locate android phone, send SMS to buzz your android cell phone. (
  • This find device and cell phone tracker app collaborates with gps tracking and keeps you informed of tracking a phone during device SIM change. (
  • By this phone tracker app now it is the matter of seconds to find phone location or track a cell phone. (
  • Track Lost Cell Phone - Find Phone Location is a reliable option for the users who have experienced the stress, worry or inconvenience in case of lost phone and were unable to track a phone. (
  • To find device or track a cell phone was not much easier than ever by the use of this gps phone tracker. (
  • Our phone tracking app collaborates and develop access to the gps locator and gps locator facilities and makes it possible to find phone instantly. (
  • Track Lost Cell Phone - Find Phone Location is capable enough to offer you all the top features of tracker phone location and find phone location features in an efficient way. (
  • Find a cell phone locate phone location just by sending SMS to the lost phone. (
  • Find cell phone location and find lost phone by using cell phone tracking just by sending SMS through a reliable phone number. (
  • This phone tracking app and cell phone tracker automatically detects the Device SIM change and locks the cell phone with security code. (
  • Track Lost Cell Phone - Find Phone Location uses a 4-6 digit security code and makes tracking app more reliable. (
  • Find phone location and cell phone tracking just by sending SMS and create a buzz. (
  • Track a cell phone by this tracker app and location tracker just by sending your phone location, lock code and IMEI number into a trusted phone number. (
  • This cell phone tracker sends SMS to your trusted phone number on Device SIM change and sends security code on your email address just for the sake of security purposes. (
  • Activation: Cell phone tracker or phone tracking app just needs to press the activation button after opening the phone tracker app. (
  • Similarly stopalarm and locate cell phone will track the lost device. (
  • There is no dispute that at least Tucson has a device that electronically mimics a cell phone tower. (
  • It is designed to work on any cell phone with Internet capabilities-except the iPhone (one of Google's competitors). (
  • It all started with a Canadian teen who left his cell phone in a taxi. (
  • In an attempt to retrieve the phone, Jeremy Cook, 18, used an app to track its location. (
  • Local police have made clear to CBC and other media that they aren't against phone-tracking apps. (
  • Over these three and a half months, the pole position traced small loops, ranging in size from that of a sheet of A4 [8-1/2x11 inch] paper down to that of a cell phone, and it remained within a one meter [yard] square during these four months. (
  • That was the warning issued to the public by several major civil liberties organizations as they appeared in federal court in Philadelphia to argue for more privacy protections in the use of cell phones as tracking devices by law enforcement agents. (
  • The FCC rules are putting into action regulations from the Communications Assistance for Law Enforcement Act, a 1994 law that requires the communications industry to design cell phones and other equipment in accordance with FBI standards to make electronic surveillance easier. (
  • It seems that law enforcement agencies are commonly using the records of people's cell phones to establish where the people (or at least their phones) were at material times. (
  • What they do is allow law enforcement to track locations in real time. (
  • "After decades of secret and warrantless use of Stingray technology by law enforcement to track phones, a federal judge has finally held authorities to account," the ACLU's Wessler said. (
  • The color-codes provided immediate visualization of cell identity and allowed us to track human cells with a success rate of 78% across image frames separated by 8 h. (
  • Since XCaps can be made using FDA-approved clinical-grade compounds, they represent the first potentially biocompatible therapy that will enable X-ray visualization of stem cells to assist in targeting cellular therapeutics," Kraitchman says. (
  • Cell tracers are frequently used in dye dilution assays to monitor cell proliferation using flow cytometry. (
  • Although current single-cell flow cytometry technologies can provide up to 17 simultaneous visualizations, this new method has the potential to do far more. (
  • In flow cytometry, cells are bombarded with laser light as they pass through a scanning chamber. (
  • On day four, cells were harvested, stained with CD3-PE and the Tag-it Violet™ signal was analyzed by flow cytometry. (
  • Cells labeled wtih Tag-it Violet™ can be analyzed by flow cytometry in instruments equipped with a violet laser, or visualized by fluorescence microscopy using a DAPI filter. (
  • This lot has been tested by flow cytometry for in vitro cell proliferation. (
  • We present a combined report on the results of three editions of the Cell Tracking Challenge, an ongoing initiative aimed at promoting the development and objective evaluation of cell tracking algorithms. (
  • A novel framework for objective evaluation of cell tracking algorithms has been established under the auspices of the IEEE International Symposium on Biomedical Imaging 2013 Cell Tracking Challenge. (
  • Cell-tracing probes are available in multiple colors for a variety of laser lines and with a full range of supporting products for applications in cell counting, viability, and proliferation assays. (
  • MRI was performed on the implants, and microcontainers retrieved postimplantation showed cell viability both within and proximal to the implant. (
  • However, lack of physiologic salts can impair cell viability over prolonged periods, and the dye aggregates will gradually form even in the absence of salts 1-3 . (
  • Therefore, it is critical to develop new imaging technologies that can non-invasively monitor the viability and the function of the transplanted stem cells in order to assess the success of the procedure and to ensure that the cells reach their intended destinations. (
  • By engineering the genome of the cells we wish to track, we can introduce molecules that can help us follow the cells with high-resolution MRI and monitor their viability. (
  • The relatively high contrast is achieved with low bismuth loading (2.66% by weight) within the US-tubes and without compromising cell viability. (
  • Nanocomplexes can be used to label transplanted cells so they can be tracked by MRI, according to a new study. (
  • Then, using a combination of mutant analysis, microscopy of FRA1 within living cells and protein biochemistry, the team showed that this interaction inhibits the movement of FRA1. (
  • Here, we survey the latest developments in this area and discuss the various computational approaches, software tools, and quantitative measures for tracking and motion analysis of cells and particles in time-lapse microscopy images. (
  • b ) Fluorescence microscopy shows intracytoplasmic localization of PFC-dye (BODIPy, red) in mouse T cells, where the nucleus is blue (Hoechst dye) and the cell surface is stained with CD4- FITC antibody (green). (
  • Tracking of ADSC was done by intratissue or intravenous transplantation of EdU-labeled ADSC into various tissues and examined by histology and fluorescence microscopy. (
  • The labeled cells display excellent image contrast in phantom imaging experiments, and transmission electron microscopy images of the labeled cells reveal the presence of highly dispersed PAA-GNTs within the cytoplasm with 10 14 Gd 3+ ions per cell. (
  • In biology, many different kinds of microscopy are used to study cells. (
  • There are many different kinds of transmission microscopy, where light is passed through the cells, that can be used without staining or other treatments that can harm the cells. (
  • Fluorescence microscopy can also be used to study particles, for example viruses, inside cells. (
  • Automatic tracking of cells in multidimensional time-lapse fluorescence microscopy is an important task in many biomedical applications. (
  • Besides performing particle tracking in 2D, there are several imaging modalities for 3D particle tracking, including multifocal plane microscopy, double helix point spread function microscopy, and introducing astigmatism via a cylindrical lens or adaptive optics. (
  • In this context, the noninvasive localization of the transplanted cells and the monitoring of their migration can facilitate basic research on the underlying mechanism and improve clinical translation. (
  • Bulte JWM, Zhang S, van Gelderen P et al (1999) Neurotransplantation of magnetically labeled oligodendrocytes progenitors: MR tracking of cell migration and myelination. (
  • In this work MR based strategies have been developed to track the migration/integration of arterially delivered muscle stem cells into skeletal muscles and the bone marrow of dystrophic mice. (
  • These wildlife tracking datasets are crucial for understanding species ranges, seasonal migration patterns, habitat use, effects of habitat disturbance, and individual behavior patterns. (
  • However, Noble points out that Snyder's work has yet to prove that the tumor cell and neural stem cells are not traveling along previously defined travel routes that force the same migration patterns. (
  • finding the signals that direct neural stem cell migration. (
  • Tracking the growth and migration of cells under hypoxic conditions in vitro can be challenging because measurements must be made in a manner that does not perturb the atmospheric composition of the closed system. (
  • This approach, wherein the number, size, attachment strength, and migration of cells are continuously monitored using gold biosensors, enabled experiments to be run from start to finish without ever needing to manipulate samples or open the hypoxia chamber. (
  • Consistent with the above findings, in hypoxic xCELLigence live cell analysis assays hYSK1 promoted both proliferation and migration of melanoma cell lines. (
  • HeLa cells were labeled with Alexa Fluor 488 Click-iT Plus EdU to indicate new DNA synthesis. (
  • We have analyzed dynamics inside two types of eukaryotic cells, fibroblasts and epithelial-like HeLa cells, with simultaneous active and passive microrheology using laser interferometry and optical trapping technology. (
  • Moreover, the cytotoxicity and cellular uptake of compounds 5 - 9 have been analyzed in vitro at different concentrations of B (5, 50, and 100 μg B/mL) using HeLa cells. (
  • immunotherapy for a tenure track position in the Cancer Biology Program (position #0088357). (
  • This is a tremendously exciting project and we've assembled a great team to work on it," said McMillen (left), which includes combines a wealth of expertise from a wide variety of areas such as synthetic biology, molecular genetics, intestinal stem cell signalling, biological/chemical engineering and bioinformatics. (
  • In recent years, single-cell biology has revealed that each cell is unique. (
  • The various organellar membranes of eukaryotic cells display striking differences in the composition, leaflet distribution and transbilayer movement of their lipids. (
  • Eukaryotic cells are compartmentalized into distinct organelles by lipid bilayers. (
  • It emphasizes the use of medical imaging for therapeutic delivery/targeting, cell tracking, and determining therapeutic efficacy. (
  • To explore the therapeutic potential of stem cells, Snyder's team engineered cells that carry a gene for the production of cytosine deaminase (CD). (
  • After two weeks, tumors that were treated with the therapeutic stem cells had shrunk by 80 percent. (
  • There are also questions as to whether these therapeutic cells cause brain damage by migrating through the brain. (
  • The next two years will be spent developing therapeutic human neural stem cells for use in clinical trials. (
  • Stem cells continue to offer great potential for therapeutic use in a variety of diseases, but it's been hard to track their effectiveness once injected. (
  • Stem cells can be used as a vehicle for continuous delivery of therapeutic agents locally. (
  • The plan states that it aims to create "an industrial stem cell therapeutic powerhouse that expands the tax base, adds high-quality jobs and increases the likelihood of the commercialization of stem cell treatments for patients with unmet needs. (
  • Often with EFF input as a friend of the court, the vast majority of judges issuing public opinions on the matter are saying "no", and rejecting government applications for cell site location tracking information made without showing sufficient need for this kind of sensitive information. (
  • But even older phones that don't have chips can be tracked by knowing the location of the cell towers they use to connect to a network," the group said. (
  • The case that concluded Wednesday concerns historical cell-site location information, which carriers usually retain for about 18 months. (
  • Li Guoguang, Deputy Director of Social Development believes that cellular location technology for real-time user tracking is an efficient way to establish a public information platform for travelling. (
  • They shuttle material in the cell from one location to another. (
  • But delivery of these cells to their target location remains a major obstacle. (
  • Did you ever wish that you could track the location of a mobile user? (
  • If the tracked user consents, the customer is then flashed with the location details of the tracked user. (
  • The request follows on the heels of revelations that federal officials are using Americans cell phones to pinpoint their location, sometimes without any court oversight. (
  • The simplest processes for monitoring cell movement and location use tracking probes that pass through the membrane into the cell and become membrane-impermeant after loading. (
  • Longer-term tracking of cell movement and location requires probes that are more resistant to photobleaching and are retained through more cycles of cell division. (
  • Using new imaging systems we can work out their precise location in the cell and how they behave over time. (
  • This offers two important advantages when compared with current strategies employed to image magnetic cores: first, it is nondestructive and is therefore suitable for studies of live cells and, second, it offers a higher sensitivity and resolution, thus allowing for the identification of low levels of SPIONs within a precise subcellular location. (
  • Sebastien Lambert of the Royal Observatory of Belgium and colleagues there and at the Paris Observatory took advantage of the opportunity to track short-term wobbles, using newly available GPS data that establish the location of the poles precisely. (
  • esearchers have shown that neural stem cells which can develop into any type of brain cell sense and migrate toward brain tumors. (
  • The team found that when these experiments were repeated using human neural stem cells, the stem cells migrated toward human brain tumor implants in the mouse brain just like their mouse counterparts. (
  • Snyder's work is an "important new contribution" that "brings us closer to the point where clinical trials will be initiated on the use of neural stem cells to treat CNS tumors," says Mark Noble, of the University of Rochester Medical Center, in a commentary accompanying the article in PNAS . (
  • The ideal stem cell would first kill the tumor cell and then differentiate into normal brain cells repairing tumor damage and reestablishing the neural architecture. (
  • Neural stem cells display extensive tropism for pathology in adult brain: Evidence from intracranial gliomas. (
  • The study will test genetically engineered neural stem cells transplanted into patients with a type of brain tumor. (
  • Melanocytes, healthy skin cells that form the pigment melanin, come from neural crest cells, which are created in the spinal column. (
  • The Company has received multiple issued patents for tipifarnib in the U.S. and foreign countries, including one issued by the U.S. Patent and Trademark Office in September 2019 that further extends Kura's exclusivity to the use of any farnesyl transferase inhibitor for the treatment of CXCL12-expressing peripheral T-cell lymphoma and acute myeloid leukemia. (
  • The city of Tucson is trying to prevent releasing documents on a device is uses similar to the one pictured, which mimics a wireless carrier cell tower to force nearby cell phones and other cellular data devices to connect to it, revealing their locations. (
  • Recent experimental evidence supports rapid flip-flop of cholesterol in PC membranes, red cell membranes and, presumably, in most other cellular membranes (reviewed in Hamilton, 2003 ). (
  • Furthermore, our work identified previously unobserved heterogeneity in the response of microglia to neurodegeneration, discovered disease stage-specific microglia cell states, revealed the trajectory of cellular reprogramming of microglia in response to neurodegeneration, and uncovered the underlying transcriptional programs. (
  • In Chapter 2, we use novel transcriptional positive-feedback based memory devices integrated in human cells to study heterogeneous responses to cellular stresses. (
  • Joint lead researcher Dr Marco Di Antonio, of Imperial, said: For the first time, we have been able to prove the quadruple helix DNA exists in our cells as a stable structure created by normal cellular processes. (
  • They first developed a way to enrich the RNA from single cells, targeting the RNAs encoding the immune cell receptors, then developed a computational tool to accurately read full-length sequences of the immune cell receptors. (
  • In this study, we have adopted this technology to analyze the lateral movement of incoming virus-like particles (VLPs) attached to their cell-surface receptors. (
  • Our study focused on murine polyoma virus (Py), a small (diameter, 45 nm), simple, nonenveloped DNA tumor virus ( 12 ) that uses gangliosides GD1a and GT1b as receptors ( 13 ) and relies on clathrin-independent, cholesterol-dependent endocytosis to deliver its genome into the cell for replication (ref. 14 and A.E.S., H.E., and A.H., unpublished observations). (
  • The approach, if it works, would be a leading example of regenerative medicine, the idea that the best way to repair the body is not with strong drugs or the surgeon's scalpel but with the body's own system of cells and signaling molecules. (
  • The difficulties of the marrow-to-heart therapy do not dash the hopes for regenerative medicine or imply failure for the stem cell research financing set up by states like California and New Jersey. (
  • Regenerative medicine focuses on using stem cells to repair and regenerate damaged tissues and organs. (
  • Last summer, the federal government gave the University of Toronto the largest single research award in its history - $114 million - to support Medicine by Design, which builds on a rich legacy of U of T contributions to regenerative medicine, starting with the identification of blood stem cells by biophysicist James Till and hematologist Ernest McCulloch in 1960. (
  • They will help strengthen U of T's reputation as a global centre in the growing field of regenerative medicine and cell therapy, power Toronto's vibrant biomedical ecosystem and - most importantly - lay the foundation for improved outcomes for patients around the world," he said. (
  • On Thursday, the California Institute for Regenerative Medicine board of directors approved a new strategic plan designed to fast-track grant proposals and shift funding to projects that bring stem cell treatments to fruition, KPCC's " KPCC News " reports (O'Neill/Glickman, "KPCC News," KPCC, 12/17). (
  • It is assumed that if an iron-labeled cell dies it will be cleared by the immune system and the area of signal loss will disappear. (
  • Although the presence of MHC-I may activate T-cells, due to lack of costimulatory molecules, MSCs fail to elicit an immune response [ 27 ]. (
  • Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase ( lck ) promoter were used to analyze critical aspects of the immune system, including patterns of T cell development and T cell homing after transplant. (
  • In the future, the technique might be used to monitor whether transplanted immune or stem cells reach their targets. (
  • They liken it to a barcode tracker, able to scan detailed information from thousands of immune cells at a time. (
  • This method gives us the most detailed view yet of how immune cells behave in the human body," says senior author Chris Goodnow, from the Garvan Institute of Medical Research. (
  • Immune cells play a critical role in the development of disease. (
  • They pinpointed a number of related cells that were present in both tissues, and which revealed specific genetic signatures of the immune response within the patient's tumour. (
  • Ideally, we'd like to be able to take donor cells off the shelf, make them x-ray visible, protect them from the immune system, and deliver them precisely where we want them to be. (
  • 19 F images of the labeled cells are displayed on a 'hot-iron' intensity scale, and the anatomical ( 1 H) images are shown in grayscale. (
  • Taken together, these findings suggest that T and B cell development in the fish relies on many of the same molecules and pathways used in mammalian lymphopoiesis ( 15 , 16 ). (
  • Recent studies in yeast, parasites such as Leishmania, and mammalian cells have identified several candidates for lipid flippases, and whereas some of these serve a fundamental role in the release of lipids from cells, others appear to have unexpected and important functions in vesicular traffic: their activities are required to support vesicle formation in the secretory and endocytic pathways. (
  • Clinical Application of Noninvasive Molecular Imaging in Cancer Cell Therapy: The First Reporter Gene-Based Imaging Clinical Trial. (
  • Here we describe the generation of a transgenic zebrafish model in which the zebrafish T cell-specific tyrosine kinase ( lck ) gene promoter drives GFP expression in lymphocytes. (
  • A microfluidic system for tracking growth and gene expression of single bacteria. (
  • As they report in Nature Communications, this integrated setup can be used to study gene regulation in single bacterial cells in response to dynamically controlled environmental changes. (
  • However, single cells can vary significantly in their gene expression. (
  • Therefore it is easier to count the number of RNA molecules in a single cell to study gene expression levels quantitatively than protein molecule numbers. (
  • We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies. (
  • In summary, there are several promising options for noninvasive cell tracking, which have different strengths and limitations that should be considered when planning cell-tracking experiments. (
  • Live-cell imaging experiments carried out to this end often produce massive amounts of time-lapse image data containing far more information than can be digested by a human observer. (
  • A recurring task in many experiments is the tracking of large numbers of cells or particles and the analysis of their (morpho)dynamic behavior. (
  • These first experiments used mouse stem cells and rat tumor implants. (
  • In transplant experiments, thymocytes from lck-GFP fish repopulated the thymus of irradiated wild-type fish only transiently, suggesting that the thymus contains only short-term thymic repopulating cells. (
  • When biologists perform experiments on cells, they often record image sequences or sequences of three-dimensional volumes to see how the cells behave when they are subjected to different drugs, culture substrates, or other external factors. (
  • The PNAS paper reports observations of 24,000 cells over the duration of the experiments. (
  • Surprisingly, the lag times are similar in genetically related cells suggesting that bacteria retain a memory of the behavior of their ancestors. (
  • In next week's Journal of Biological Chemistry "Paper of the Week," Roger Gomis and colleagues at the Institute for Research in Biomedicine in Spain investigated how breast cancer cells migrate to bone. (
  • Radionuclide Approaches to Imaging Stem Cells and Their Biological Effects on the Myocardium. (
  • The complex interior of a biological cell is an active system because it is maintained away from thermodynamic equilibrium by internal energy dissipation and, in particular, by a variety of force-generating machineries ( 1 , 2 ). (
  • Two primary components determine the mechanics of a cell: the cytoskeleton, a network of semiflexible protein polymers, and the cytoplasm, a concentrated solution of biological macromolecules and organelles. (
  • This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism. (
  • While we are still emerging from the foundational stages of this new field, we are already using engineered cells to discover underlying biological mechanisms, develop new therapeutics, and produce natural products. (
  • Now we can track G4s in real time in cells we can ask directly what their biological role is. (
  • The small amounts meant they couldn t hope to image every G4 in a cell, but could instead identify and track single G4s, allowing them to understand their fundamental biological role without perturbing their overall prevalence and stability in the cell. (
  • This thesis presents algorithms to find cells and particles in images, and to calculate tracks that show how they have moved during an experiment. (
  • We selected and extended a number of existing segmentation algorithms, and thereby created a complete tool to find cell outlines. (
  • Photothermal technique, a unique optical imaging system is used to improve SPION labelling so that particles survive for longer and have minimal impact on the function of the transplanted cells. (
  • EFF explains that, although most people don't realize it, cell phones double as tracking devices. (
  • Cell Phones - Good for Tracking People? (
  • The situation is similar in many cities - residents didn't know their cell phones could be tracked. (
  • Although better locating data is available with DGPS ( Differential GPS ) (accuracy to within three to five meters), this capability has not been incorporated into the majority of cell phones at the time of this writing. (
  • What Factors Degrade the GPS Accuracy of Cell Phones? (
  • NEW YORK - The American Civil Liberties Union filed a Freedom of Information Act (FOIA) request today with the Department of Justice (DOJ) to learn the details of the government s use of cell phones as tracking devices. (
  • We have a right to know if, and for how long, the government has been using cell phones to track people in this invasive manner, said Crump. (
  • The only way shoppers can opt out is by turning off their cell phones. (
  • Google Latitude is one free service that keeps track of your contacts by locating their cell phones and displaying their whereabouts on a Google Map. (
  • Employers increasingly want to know where employees are during business hours, so company-issued GPS phones equipped with tracking software are one solution. (
  • Before LocationSmart's demo was taken offline today, KrebsOnSecurity pinged five different trusted sources, all of whom gave consent to have Xiao determine the whereabouts of their cell phones. (
  • This unusual case involved federal agents and police officers nationwide using secret surveillance technology to spy on citizens' cell phones during criminal investigations. (
  • In 2014, the U.S. Supreme Court ruled warrantless search of cell phones illegal. (
  • Electronic Frontier Foundation Digital Rights Analyst Rebecca Jeschke told NewsBlaze that if the government's appeal is successful that the effects upon cell phones will only further erode privacy. (
  • As we use cell phones more and more, a successful appeal will touch nearly every American. (
  • A Stingray device is a generic term that refers to any cell-site simulator with capability to mimic cell towers to find cell phones that police targeted. (
  • According to ACLU reports, at least sixty government agencies in 23 states have been using the Stingray to sweep up data off citizen's cell phones. (
  • While U.S. malls have long tracked how crowds move throughout their stores, this is the first time they've used cell phones. (
  • I'm sure as more people get more cell phones, it's probably inevitable that it will continue as a resource,' Mulpuru said. (
  • Tucson police purposely hides its use of technology that allows it to track the cell phones of people - innocent or otherwise - the American Civil Liberties Union is charging. (
  • Tucson police have said they use it to track specific suspects by their phones, ignoring other numbers and taking the device, which is portable, from door to door if necessary. (
  • Does Google offer free tracking software for cell phones? (
  • A new piece of free Google software allows people to keep track of each other using their cell phones-and while it is opt-in, it is sure to create a privacy firestorm. (
  • Google Latitude, a free piece of software released in February 2009, allows people to keep track of each other using their cell phones. (
  • We have recently introduced the method of particle-tracking microrheology, which measures the viscoelastic properties of the cytoplasm locally and with high spatiotemporal resolution. (
  • Here we establish the basic principles of particle-tracking microrheology, describing the advantages of this approach over more conventional approaches to cell mechanics. (
  • Particle-tracking microrheology can probe the mechanical properties of live cells in experimentally difficult, yet more physiological, environments, including cells embedded inside a 3D matrix, adherent cells subjected to shear flows, and cells inside a developing embryo. (
  • Particle-tracking microrheology can readily reveal the lost ability of diseased cells to resist shear forces. (
  • Single-particle tracking (SPT) is the observation of the motion of individual particles within a medium. (
  • Additionally, single-particle tracking has been extensively used in the study of reconstituted lipid bilayers, intermittent diffusion between 3D and either 2D (e.g., a membrane) or 1D (e.g., a DNA polymer) phases, and synthetic entangled actin networks. (
  • On a fundamental level, once the images are obtained, single-particle tracking is a two step process. (
  • The stem cells injected directly into the tumor infiltrate the entire growth and migrate just outside the border of the tumor to surround or encapsulate it. (
  • Its mazelike design allows a group of cells to sort themselves into a starting lineup then migrate across a set of tight parallel tunnels whose narrowness mimics that of the lymphatic vessels that metastatic cells have to travel through. (
  • However, cellassociated dye aggregates are not efficiently removed by washing and act as reservoirs of unbound dye that can transfer to unlabeled cells present in an assay. (
  • Soll's team, in a paper published in PLOS One in March 2015, previously showed that human breast cancer cells form tumors by extending cables--bridges of sorts--between small aggregates of cancer cells. (
  • It upholds our hypothesis that coalescence is so similar that there's got to be the same molecules and mechanisms that do it, and we may be able to find a drug that shuts tumor formation down without being toxic to healthy cells in the body,' says Soll, the paper's corresponding author. (
  • As metabolites are usually small molecules, typically cells have to be destroyed and the metabolome extracted in order to record such changes. (
  • The dynamics of RNA molecules including mRNAs can now be studied by tracking single RNA molecules. (
  • reviewed the current state-of-the-art technology for tagging, delivery, and imaging useful for the tracking of single mRNA molecules in live cells. (
  • Single molecule analysis of RNA may enable counting numbers of mRNA molecules inside single cells. (
  • Tracking single mRNP molecules in real time may allow studying the sequence of events or RNA processing and transport. (
  • How many molecules are there in a cell? (
  • 2016), in chicken embryonic fibroblast cells there are approximately 2500 mRNA molecules present per cell, whereas there are approximately 10 8 β-actin molecules present per cell. (
  • Invitrogen Qtracker probes provide intense, stable signals for long-term (6-10 generations) tracking. (
  • We offer Invitrogen HCS CellMasks for whole cell demarcation in a variety of wavelengths for multiplexing together with other structural and functional probes. (
  • We developed a method to track microscopic probes stably in cells in the presence of vigorous cytoplasmic fluctuations, by using smooth three-dimensional (3D) feedback of a piezo-actuated sample stage. (
  • They tested their new calcium sensor with one of the optogenetic probes (channelrhodopsin) that causes the calcium ion channels in the neuron's outer membrane to open, flooding the cell with calcium. (
  • However, the technique used to require either killing the cells or using high concentrations of chemical probes to visualise G4 formation, so their actual presence within living cells under normal conditions has not been tracked, until now. (
  • The opinion is clear that the government can access cell site records without Fourth Amendment oversight ," Kerr told the New York Times this week. (
  • Owing to their rapid proliferation, the cells within a solid tumor often outpace the ability of the surrounding vasculature to provide nutrients and oxygen. (
  • Tag-it Violet™ Proliferation and Cell Tracking Dye Rated 5 / 5 based on 2 reviews. (
  • Human peripheral blood mononuclear cells were stained with Tag-it Violet™ Proliferation and Cell Tracking dye, and then stimulated with PHA for four days. (
  • Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. (
  • The Tag-it Violet™ Proliferation and Cell Tracking Dye is composed of lyophilized Tag-it Violet™ and anhydrous DMSO. (
  • The Tag-it Violet™ Proliferation and Cell Tracking Dye should be stored at -20°C upon receipt. (
  • The molecular weight of Tag-it Violet™ Proliferation and Cell Tracking Dye is 489 kD. (
  • The maximum excitation and emission wavelengths of Tag-it Violet™ Proliferation and Cell Tracking Dye are 395 nm and 455 nm, respectively. (
  • Each 122.25 µg of Tag-it Violet™ Proliferation and Cell Tracking Dye may be reconstituted with 50 µl of anhydrous DMSO to yield a stock concentration of 5 mM. (
  • EMR rays in general cause irritation, concentration lapses and in many cases even proliferation of cells which cause cancer," said Dr Rajeev Ranjan, a New Delhi neurologist. (
  • The best-studied transplanted stem cell is the hematopoietic stem cell (HSC) that can be harvested from different sources (bone marrow, blood, and umbilical cord blood) in sufficient numbers for transplantation. (
  • Cell therapy can be defined as the transplantation of living cells for the treatment of medical disorders. (
  • Tracking of ADSC was successful in tissues 10 weeks after intratissue or intravenous transplantation. (
  • Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. (
  • To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. (
  • Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication charge, which is dependent on the number of pages, in addition to the charge for color figures. (
  • Cell Transplantation is now being published by SAGE. (
  • In order to fully explore this potential, however, more technological developments are needed to understand how stem cells behave in the body after transplantation. (
  • Before receiving injections of their bone marrow stem cells, most of the patients were bedridden or too sick to walk without effort. (
  • Not only could they label different metabolites with different colors, but they were also able to follow their uptake profiles in cancer cells simultaneously. (
  • Civil liberties groups are asking the court to require that the government show probable cause before it can track your whereabouts. (
  • In those cases, the police are tracking the whereabouts of not just suspected criminals, but potentially thousands of third-party innocent citizens. (
  • Giving the government the power to track and store a person s whereabouts at any given moment without probable cause is a serious intrusion on our personal privacy, said Catherine Crump, staff attorney for the ACLU. (
  • But they do suggest that successful stem cell treatments, whether with adult cells or ones derived from embryos, may require many years to come to fruition. (
  • This is important for tracking of some particles inside cells and for tracking of cell nuclei in some embryos. (
  • A process that normally occurs in developing embryos - the changing of one basic cell type into another - has also been suspected of playing a role in cancer metastasis. (
  • MSCs are characterized as adherent cells with the ability to differentiate into fibroblasts, adipocytes, chondrocytes, osteocytes, and smooth muscle cells apart from supportive hematopoietic "stromal" cells [ 1 , 3 , 4 , 9 ] and with a characteristic cell surface antigen profile. (
  • The team found GNTs did not affect the stem cells' ability to differentiate into other types of cells or to self-renew, though work continues to characterize their ability to adhere to cell scaffolds under various conditions. (
  • Nevertheless, feasibility of clinical translation has been achieved by showing the localization of USPIO-labeled dendritic cells in cervical lymph nodes of patients by clinical MRI. (
  • In the final chapters, leading scholars offer clinical perspectives on existing and potential uses of stem cells as well as the impact of image-guided delivery and tracking in major organ systems. (
  • Clinical Cardiology Stem Cell Applications. (
  • Musculoskeletal Clinical Applications of Stem Cells. (
  • For stem cells to be used in the clinical setting, they should be safe, that is, do not form tumors, and be readily harvested and/or expanded. (
  • We also address the potential applicability of (19) F cell tracking to clinical trials. (
  • A research team led by Dr. Joseph A. Frank of NIH's Clinical Center and National Institute of Biomedical Imaging and Bioengineering (NIBIB) sought to develop an alternative method of tracking cells using MRI. (
  • But clinical agents like the gadolinium-based Magnevist cannot enter cells. (
  • Several clinical trials in humans were conducted around the world, showing the importance of tracking the cells in the body. (
  • Pre-clinical and clinical studies have shown that hypericin combined with photoactivation can have significant antiproliferative effects on activated normal lymphoid cells and can contribute to inhibiting the growth of malignant T-cells in patients diagnosed with CTCL. (
  • Anderson SA, Shukaliak-Quandt J, Jordan EK et al (2004) Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis. (
  • Live and fixed cell imaging demonstrated that mRNAs can extend beyond the protein surface of a stress granule, which may facilitate interactions between RNP granules. (
  • It then describes the main imaging modalities and state-of-the-art techniques that are currently employed for stem cell tracking. (
  • Bioluminescence, MRI, and PET Imaging Modalities of Stem Cell-Based Therapy for Neurological Disorders. (
  • The development of relevant imaging tools will lead to a better understanding of how cell therapy works, the possibility of response monitoring in patients, and sufficient safety of the treatment. (
  • Delivering stem cells to specific tissues is a very big challenge, since basically all investigators have to shoot blindly," says Sanjiv Gambhir , director of Stanford's molecular imaging program who led the work published online today in Science Translational Medicine . (
  • The study has determined GNTs are effective in helping doctors track stems cells through the body by making them 40 times better than standard contrast agents used in magnetic resonance imaging. (
  • Tests show GNTs are highly effective for tagging and tracking stem cells through magnetic resonance imaging. (
  • We have shown that this technique may be applied to the imaging of both cell monolayers and cryosections. (
  • Why is imaging of RNAs in living cells useful? (
  • We have developed a complete system that can find and track cells in all commonly used imaging modalities. (
  • We present in this paper a Region Of Interest (ROI) tracking algorithm specialized for confocal imaging using a scanning device. (
  • According to biopharmaceutical company Soligenix, the US Food and Drug Administration has fast-tracked the company's experimental treatment SGX301 for the front-line treatment of patients with cutaneous t-cell lymphoma (CTCL). (