Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Endosseous dental implantation where implants are fitted with an abutment or where an implant with a transmucosal coronal portion is used immediately (within 1 week) after the initial extraction. Conventionally, the implantation is performed in two stages with more than two months in between the stages.
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The fifth planet in order from the sun. It is one of the five outer planets of the solar system. Its sixteen natural satellites include Callisto, Europa, Ganymede, and Io.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Viruses whose hosts are bacterial cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS.
Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
The study of natural phenomena by observation, measurement, and experimentation.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
A generic concept reflecting concern with the modification and enhancement of life attributes, e.g., physical, political, moral and social environment; the overall condition of a human life.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Compounds that contain the radical R2C=N.OH derived from condensation of ALDEHYDES or KETONES with HYDROXYLAMINE. Members of this group are CHOLINESTERASE REACTIVATORS.
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Standards for limiting worker exposure to airborne contaminants. They are the maximum concentration in air at which it is believed that a particular substance will not produce adverse health effects with repeated daily exposure. It can be a time-weighted average (TLV-TWA), a short-term value (TLV-STEL), or an instantaneous value (TLV-Ceiling). They are expressed either as parts per million (ppm) or milligram per cubic meter (mg/m3).
The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)
An institute of the CENTERS FOR DISEASE CONTROL AND PREVENTION which is responsible for assuring safe and healthful working conditions and for developing standards of safety and health. Research activities are carried out pertinent to these goals.
Exclusive legal rights or privileges applied to inventions, plants, etc.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The visual display of data in a man-machine system. An example is when data is called from the computer and transmitted to a CATHODE RAY TUBE DISPLAY or LIQUID CRYSTAL display.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Information systems, usually computer-assisted, designed to store, manipulate, and retrieve information for planning, organizing, directing, and controlling administrative activities associated with the provision and utilization of radiology services and facilities.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
A chemical element having an atomic weight of 106.4, atomic number of 46, and the symbol Pd. It is a white, ductile metal resembling platinum, and following it in abundance and importance of applications. It is used in dentistry in the form of gold, silver, and copper alloys.
Any chemical species which accepts an electron-pair from a LEWIS BASE in a chemical bonding reaction.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Changing an open-chain hydrocarbon to a closed ring. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Unsaturated hydrocarbons of the type Cn-H2n, indicated by the suffix -ene. (Grant & Hackh's Chemical Dictionary, 5th ed, p408)

Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes. (1/81)

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Relevant uses of surface proteins--display on self-organized biological structures. (2/81)

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SplitCore: an exceptionally versatile viral nanoparticle for native whole protein display regardless of 3D structure. (3/81)

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Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display. (4/81)

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Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin. (5/81)

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Bacillus subtilis: a temperature resistant and needle free delivery system of immunogens. (6/81)

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Baculovirus as a vaccine vector. (7/81)

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Surface display of N-terminally anchored invasin by Lactobacillus plantarum activates NF-kappaB in monocytes. (8/81)

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The unique property of specific high-affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. Hence knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. Here, we describe a high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen fragments followed by antibody-based flow cytometric sorting. Epitopes are determined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. The method described here has been useful for the mapping of both monoclonal and polyclonal antibodies with varying sizes of epitopes.. ...
National Pingtung University of Science and Technology - Faculty Open Information System. | 國立屏東科技大學教師資訊公開查詢系統,提供教師個人、授課、著作及研發績效等資訊查詢
A new technique, dubbed prime editing, appears to make it even easier to make very precise changes in DNA. Its designed to overcome the limits of the CRISPR gene editing tool.
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins.. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
A biorefinery is a special type of refinery in which biomass, such as plant cellulose, is converted by microorganisms into useful products. Edinburghs 2011 iGEM project is a feasibility study into the creation of biorefineries using E. coli, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create synergy between them. We investigated two methods of bringing the enzymes close together: cell surface display via Ice Nucleation Protein, and phage display via M13s major coat protein. We attempted a new DNA assembly protocol, provisionally named BioSandwich. We constructed computer models of synergy to assess whether it is a feasible option. Finally, we considered the broader economic and social questions surrounding the construction of a biorefinery: can it be done, and should it be done? ...
TY - JOUR. T1 - Automated panning and screening procedure on microplates for antibody generation from phage display libraries. AU - Turunen, Laura. AU - Takkinen, Kristiina. AU - Söderlund, Hans. AU - Pulli, Timo. PY - 2009. Y1 - 2009. N2 - Antibody phage display technology is well established and widely used for selecting specific antibodies against desired targets. Using conventional manual methods, it is laborious to perform multiple selections with different antigens simultaneously. Furthermore, manual screening of the positive clones requires much effort. The authors describe optimized and automated procedures of these processes using a magnetic bead processor for the selection and a robotic station for the screening step. Both steps are performed in a 96-well microplate format. In addition, adopting the antibody phage display technology to automated platform polyethylene glycol precipitation of the enriched phage pool was unnecessary. For screening, an enzyme-linked immunosorbent assay ...
Phage display technology for identifying selective antigens and address molecules on brain endothelial cells. In Blood Barrier: Biology and Research Protocols (Ed. S. Nag)
Creative Biolabs is one of the well-recognized expert who is professional in applying various phage display technologies to offer library construction and screening related services for a broad range of project objectives.
Healing From The Cause uses a unique and powerful technique called Holographic Kinetics. It enables issues to be fully cleared at their cause.
Healing From The Cause uses a unique and powerful technique called Holographic Kinetics. It enables issues to be fully cleared at their cause.
Based on years of experience in phage display technology, Creative Biolabs now is able to provide services for the construction and screening of immune antibody library.. With years of research and development in the past decades, phage display has been a powerful technology to display millions or even billions of different peptides or proteins. It is now a common choice for the studies of protein-protein, protein-peptide and protein-DNA interactions. Among all the applications of phage display technology, one of the most successful ones is the isolation of monoclonal antibodies utilizing large capacity phage antibody libraries.. Thanks to the rapid development of this technology, it has become a reliable tool for the production of monoclonal antibody with high specificity and affinity. Compared with conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners. This expands the possibility to develop monoclonal antibodies ...
Having been researching in the field of phage display technology for years, Creative Biolabs is able to offer a series of comprehensive phage display library screening services by its own biopanning strategies and enhanced equipments.. Phage display technology is one of the major approaches applied in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds and ligands. As binders with the desired specificity generally exist at low frequencies in the constructed libraries, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.. Libraries from customized library construction services, in-house premade antibody libraries, peptide libraries or scaffold libraries, libraries provided by clients and other commercialized libraries can all be screened in Creative Biolabs.. No matter its purified or unpurified targets, we can screen for ...
INTRODUCTION Cancer of the testes is currently the most frequent neoplasm and a leading cause of morbidity in men 15-35 years of age. Its incidence is increasing. Embryonal carcinoma is its most malignant form, which either may be resistant or may develop resistance to therapies, which results in relapses. Cancer stem cells are hypothesized to be drivers of these phenomena. SPECIFIC AIM The specific aim of this work was identification and isolation of spectra of single, living cancer stem cells, which were acquired directly from the patients biopsies, followed by testing of their pluripotency. PATIENTS METHODS Biopsies were obtained from the patients with the clinical and histological diagnoses of the primary, pure embryonal carcinomas of the testes. The magnetic and fluorescent antibodies were genetically engineered. The SSEA-4 and TRA-1-60 cell surface display was analyzed by multiphoton fluorescence spectroscopy (MPFS), flow cytometry (FCM), immunoblotting (IB), nuclear magnetic resonance
Buy Eddie Cochran - Somethin Else / Boll Weevil Song - Liberty - 7, Single, Promo - F-55203, includes Somethin Else, Boll Weevil Song
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Paroles de boll weevil chanson de the presidents of the usa? Trouvez sur www.parolesbox.fr toutes les paroles de the presidents of the usa dont la musique boll weevil
Boll Weevil is a minor antagonist from the TV series DuckTales. He is an evil, ruthless, anthropomorphic dog and a greedy oat tycoon and farmer. He is the main villain of the episode "Horse Scents." Boll Weevil first appears after Mr. Merriweathers camera breaks in an untimely accident, and he...
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described
Modeling of disease pathogenesis and immunity often is carried out in large animals that are natural targets for pathogens of human or economic relevance. Although murine mAbs are a valuable tool in identifying certain host/pathogen interactions, progress in comparative immunology would be enhanced by the use of mAbs isolated from the host species. Such antibodies would reflect an authentic host immune response to infection or vaccination, and as they are host derived, would allow the application of in vivo experiments that previously have been unrealizable in large animals because of induction of an antispecies immune response. The advent of antibody phage display technology provides a way of producing host-derived mAbs in animals where the molecular genetics of Ig formation are known. Exploiting recent advances in the molecular immunology of cattle, we report here the design of an optimized phage display vector, pComBov, for the construction of combinatorial libraries of bovine Ig ...
A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these...
The boll weevil is not much to look at, but it was powerful enough to forge an unprecedented partnership between farmers, legislators and scientists.
In 1915, Floridas agricultural economy was in serious trouble. Its principal cash-crop, cotton, was being devastated by the boll weevil, an invasive beetle that crossed over from Mexico in the early 1900s. Farmers were desperate to make up… Read More. ...
EXECUTIVE INN & SUITES in Enterprise AL at 615 Boll Weevil 36330 US. Check reviews and discounted rates for AAA/AARP members, seniors, groups & military/govt.
Last night for dinner we went to Boll Weevil for the best deal in town. All you can eat fish fry for only $7.99. With coupon its only $5.99! Hard to believe, I know. And you can tell its good quality fish, not processed institutional patties. Includes a salad, oddly served in a wooden bowl.…
VacantLand property for sale in Enterprise,AL (MLS #465572). Learn more from Team Linda Simmons Real Estate. GREAT COMMERCIAL LAND WITH DEEP LOTS.
В данном разделе предоставлен видео клип Bollweevils The - The Boll Weevils Для большинства клипов также доступны тексты песен и переводы. Полный список видео клипов Bollweevils The расположен во вкладке Видео. Все клипы собирается в автоматическом режиме, если предоставленный материал не соответствует заявленному, вы можете уведомить нас об этом используя специальный блок над клипом. Вы также можете добавить свою версию видео клипа Bollweevils The - The Boll Weevils ...
These terms and conditions apply between you, the User of this Website (including any sub-domains, unless expressly excluded by their own terms and conditions), and KEVOM LT, the owner and operator of this Website. Please read these terms and conditions carefully, as they affect your legal rights. Your agreement to comply with and be bound by these terms and conditions is deemed to occur upon your first use of the Website. If you do not agree to be bound by these terms and conditions, you should stop using the Website immediately.. In these terms and conditions, User or Users means any third party that accesses the Website and is not either (i) employed by KEVOM LT and acting in the course of their employment or (ii) engaged as a consultant or otherwise providing services to KEVOM LT and accessing the Website in connection with the provision of such services.. You must be at least 18 years of age to use this Website. By using the Website and agreeing to these terms and conditions, you represent ...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells surfaces lead to the identification of unknown tumoral markers. Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. Many Ab molecules directly or indirectly targeting
Data from a survey were used to evaluate Tennessee farmers willingness to pay for the boll weevil eradication program. Producer experience, boll weevil control costs, and attitudes about boll weevil damage and insecticide usage after the program were significant explanatory variables and had a positive influence on willingness to pay. Larson, James A.; Collins, Rebecca L.; Roberts, Roland K.; English, Burton C.
Get information, facts, and pictures about boll weevil at Encyclopedia.com. Make research projects and school reports about boll weevil easy with credible articles from our FREE, online encyclopedia and dictionary.
Cole Sears 10-yard touchdown run with 1:44 remaining sent the University of Arkansas-Monticello to a 46-45 Great American Conference win over nationally 24th-ranked Arkansas Tech University at Convoy Leslie Cotton Boll Stadium in Monticello on Saturday.. Josh Marini added the extra point following Sears scamper for the ninth and final lead change of the day.. Arkansas Tech (4-2 overall, 4-2 GAC) turned the ball over on downs at its own 39-yard line with 1:11 remaining and the Boll Weevils (3-3, 3-3) ran out the clock.. The first half alone saw five lead changes and a combined 492 yards of total offense.. Techs Ty Reasnor and UAMs Sears both threw a touchdown pass in the first quarter to forge a 7-7 tie after 15 minutes.. The Boll Weevils took their first lead of the day on a 1-yard touchdown run by Jalen Tolliver at the 10:43 mark of the second quarter. The extra point try was no good, leaving UAM with a 13-7 edge.. Justin Bailey scored his first career rushing touchdown as a Wonder Boy and ...
The Cotton Boll Weevil, by Franklin Sherman (Extension Circular No. 104), and North Carolina Boll-Weevil Program for 1924, by Professors C. B. Williams and Franklin Sherman (Extension Folder No. 14); Cooperative Extension Pamphlets - The Cotton Boll Weevil, :: Cassius Rex Hudson ...
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The Boll Weevil, cotton in Texas, and Tex Ritter; by Archie McDonald, PhD., All Things Historical, an East Texas syndicated historical column.
Texas Boll Weevil Eradication located at Abilene, TX - find the revenue, phone#, the employees list and their detailed information including the job titles and emails, and much more on LeadFerret.com.
We at FAWE have built this platform to aid learners, trainers and mentors get practical help with content, an interactive platform and tools to power their teaching and learning of STEM subjects, more. ...
I met Kathy trying to sell our first house. It had been on the market with another agent and it just wasnt being looked at. After six months or more I found Kathy and she helped me stage my home. Within two weeks we began to have numerous viewings and... (more). I met Kathy trying to sell our first house. It had been on the market with another agent and it just wasnt being looked at. After six months or more I found Kathy and she helped me stage my home. Within two weeks we began to have numerous viewings and quickly sold our home. She was very helpful in explaining to my three young sons the importance of keeping their rooms clean and supporting the get the house ready to be viewed concept. She reminded them that we were selling this house to move into our new home out of state and be reunited with their dad when he came home from being deployed. It worked, they got so good at it that I could just call them from work and tell them that someone was coming to look at the house, to clean it up ...
Listing Agent: Jackie Thompson. Courtesy of: CENTURY 21 Regency Realty, Inc.. Office Phone: 3343470048. Updated: 22nd January, 2018 5:34 AM.. ...
VacantLand property for sale in Enterprise,AL (MLS #174710). Learn more from Team Linda Simmons Real Estate. Next to State Employment Center, close to High School.
Jack Dailey, a former student from my teaching days, checks in with Part 1 of the story of his family farm in Louisiana, and he sends us a killer recipe for spatchcocked Cornish game hens, which taste much better once you know what spatchcocked actually means.. ...
Jack Dailey, a former student from my teaching days, checks in with Part 1 of the story of his family farm in Louisiana, and he sends us a killer recipe for spatchcocked Cornish game hens, which taste much better once you know what spatchcocked actually means.. ...
Dear Thor!, I exclaimed. I had solved the most annoying problems in DNA chemistry in a single lightening bolt. Abundance and distinction. With two oligonucleotides, DNA polymerase, and the four nucleosidetriphosphates I could make as much of a DNA sequence as I wanted and I could make it on a fragment of a specific size that I could distinguish easily. Somehow, I thought, it had to be an illusion. Otherwise it would change DNA chemistry forever. Otherwise it would make me famous. It was too easy. Someone else would have done it and I would surely have heard of it. We would be doing it all the time. What was I failing to see? Jennifer, wake up. Ive thought of something incredible. --Kary Mullis from his Nobel lecture; December 8, 1983 The PCR primers you will use today recognize sequences flanking the Aga2 fusion on the yeast display plasmid. After performing PCR, the DNA produced will be mixed with yet another primer that will serve as a starting point for the sequencing reactions. ...
The project „BiCleanuses fingerprint sensors as an example, offering an innovative technical tool for the detection and removal of biofilms and thus preventing the risk of the transmission of pathogenic germs. Bidirectional displays have been coated with titanium dioxide (TiO2) or TiO2-containing layer combinations, thus the biofilm can be inactivated.. Bidirectional displays can emit light or content as well as detect and evaluate the incoming light via an integrated camera function. The development of this display technology is one of the core competencies of the Fraunhofer FEP. The bidirectional characteristic allows the recording of the surface status by the device itself.. This makes it possible to detect the formation of biofilms and then start a cleaning interval depending on the degree of contamination. Another research focus is the cleaning of the surface, which will also be done by light emission from the display. This is where another core competence of the Fraunhofer FEP comes ...
TY - JOUR. T1 - Rapid simultaneous screening for DNA integrity and antigen specificity of clones selected by phage display. AU - Fischer, P.. AU - Leu, S. J.C.. AU - Yang, Y. Y.. AU - Chen, P. P.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1994. Y1 - 1994. UR - http://www.scopus.com/inward/record.url?scp=0028180282&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028180282&partnerID=8YFLogxK. M3 - Article. C2 - 7520712. AN - SCOPUS:0028180282. VL - 16. SP - 828+830. JO - BioTechniques. JF - BioTechniques. SN - 0736-6205. IS - 5. ER - ...
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Feature: In the second part of our in-depth LCoS investigation, youll learn about or test methodology and equipment, and well ...
Some of you may be wondering exactly how Electroluminescent Display Technology can be used for your application. It seems the folks at EF Johnson Technologies have
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Id like to update you on the progress of my Agency, the U.S. Department of Agricultures Animal and Plant Health Inspection Service, and our partners in the States and cotton industry have made towards completely eradicating boll weevil from the United States. More than 30 years ago, you could find boll weevils-a tiny, long-snouted, persistent pest- in every cotton-producing state from Virginia to Texas. This beetle migrated from Mexico to the United States in the 1890s, feasting on our countrys abundant cotton crop. The boll weevil has cost Americas cotton producers more than $15 billion in yield losses and control efforts. Through the cooperative boll weevil eradication program, APHIS and its partners have spent nearly $1 billion fighting this damaging pest, helping cotton growers rid their crop of this pest and increasing their production. This eradication program has been based on timed sprays during growing season and ensuring cotton is completely removed approaching winter so it ...
Boll Weevils Baseball Camps are held at Blossoms Field on the University of Monticello campus in Monticello, Arkansas. Boll Weevils Baseball Camps are led by Head Coach John Harvey and Boll Weevil baseball staff.
Press Release issued Oct 16, 2017: Persistence Market Research (PMR), in its latest report, projects that the global antibody library technologies market revenue will increase at a CAGR of 4.4% between 2016 and 2024. Some of the latest antibody library design and selection methods enable easy identification of any MAB with any specificity, which is why pharmaceutical companies are increasingly making use of such technologies in their facilities.
The boll weevil is a beetle measuring six millimeters, which feeds on cotton buds and flowers. National Geographic is using this Boll Weevil in their textbook for American & World History | National Geographic Store (National Geographic Learning).
article{c02feb63-0c75-498d-9b63-6043d6df0ab2, abstract = {Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure ...
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.in.gov/portal/The-insect-enemies-of-the-cotton-boll-weevil-by/hrnHcC9YI00/ typeof=Book http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.in.gov/portal/The-insect-enemies-of-the-cotton-boll-weevil-by/hrnHcC9YI00/,The insect enemies of the cotton boll weevil, by W. Dwight Burgess, assisted by R.A. Cushman and C.E. Hood,/a,,/span, - ,span property=potentialAction typeOf=OrganizeAction,,span property=agent typeof=LibrarySystem http://library.link/vocab/LibrarySystem resource=http://link.library.in.gov/,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.in.gov/,Indiana State Library,/a,,/span,,/span,,/span,,/span,,/div ...
Find out how much you can pawn off your stuff for in Little Rock. Interest and fees pawn calculator, instant pawn price estimator. Reviews, tips and recommendations about cash loans, cash for gold service and inventory in Boll Weevil Pawn Shop pawn shop from other customers.
Advertisement for Toxaphene featuring a photograph of a boll weevil. Originally trademarked by the Hercules Powder Company, Toxaphene was a synthetic organic insecticide toxicant primarily used in agricultural…. ...
Our scientists perform extensive and careful designing of the desired sequence for codon optimization, randomization, and mutation, insertion of reporter gene sequences or tags to facilitate subsequent detection /purification of the desired ligand, cDNA or the antibody. For cDNA phage display libraries, Rx Biosciences uses a novel protocol, which avoids restriction enzyme mediated cDNA truncation, as commonly used by other vendors for cDNA cloning. Hence our protocol enhances the chance of display of full length proteins at the surface of the phages. Rx Biosciences also constructs normalized, subtracted and full-length cDNA libraries in different phage display vectors. We pioneer in recombinant antibody library construction, screening and overexpression for large scale isolation and purification of monoclonal antibodies.. To receive a custom quote, please contact us. We look forward to hearing from you!. Receive a Quote ...
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins. ...
Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. In two of the projects, a three-helix protein domain based on staphylococcal protein A has been used as scaffold to generate so called Affibody molecules capable of binding to key proteins related to two diseases common among elderly people.. In the first project, Affibody molecules were selected using phage display technology for binding to Ab-peptides, believed to play a crucial role in Alzheimers disease, in that they can oligomerize and contribute to the formation of neural plaques in the brain. The selected Affibody molecules were found to efficiently capture Ab from spiked human plasma when coupled to an affinity resin. The ...
The term Boll weevil as it applies to the area of agriculture can be defined as An insect pest of cotton that is the subject of an Animal and Plant Health Inspection Service eradication program cooperatively funded and managed by cotton producers. ...
or https:// means youve safely connected to the .gov website. Share sensitive information only on official, secure websites. ...
Pixdaus.com is an environmental photo-bookmarking and sharing service. All the pictures are uploaded by users and the copyrights belong to the rightholders. You can request a removal using the Copyright flag link below each image if you find an image that shouldnt belong here ...
by Deanna , Dec 5, 2017 , ABCs of Scripture and Stories of Christians Who Lived Them. How do we measure success? Usually its how much money we make or the quality of the things we own. Perhaps the recognition and accolades we receive from others. However, in light of Eternity, we should be measuring it differently. In my next installment of The ...
Most topiary gardens that you see are in the shapes of animals or teapots. Not Pearls garden. His topiary is free flowing and surprising. He also branched out and started creating metal sculptures to add to his garden. As we were walking around the house and garden, Pearl himself came out and talked to us. He told us that he grew up in a sharecropper family where he would get beaten for being creative and making things out of found objects. He now gives lectures at universities and has a positive outlook on life. Bishopville is sort of out of the way - actually it is way out of the way, but Pearls garden is something really worthwhile to see. These pictures are just a small sample of what he has in his ex-cornfield ...
John Rollin Ridge or Yellow Bird (1827 - 1867), a citizen of the Cherokee Nation, is considered the first Native American novelist. Ridge was the son of Treaty Party leader John Ridge and the grandson of Major Ridge, longtime Speaker of the Cherokee Nation.
In condimentum facilis porta. Sed nec diam eu diam mattis viverra. Nulla fringilla, orci acerty euismod semper, magna diamert porttitor mauris, quis sollicitudin sapien justo in libero. Mei iriure dignissim ad, prompta dissentiunt te quo. His ut elitr ceteros ...
Thursday, October 28, 2010 @7:51:26 PM thanks Tuneager. i cant wait to jam with ya! hopefully my schedule will be a little more open soon.. ...
I doubt I could ever eat squirrel stew though I have no problem seeing those spawns of Satan in a stew pot!! I remember Granny on The Beverly Hillbillies cooking it. The spawns broke a pot yesterday. They are back in my sights!!. ...
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SongPop 2 is the worlds best music trivia game featuring more than 100,000 songs from your favorite musicians!. iOS 7.0, Android 2.3 ...
Nitin Kulkarni, president of Corning Display Technologies Taiwan, confirmed at FPD Taiwan 2006 that Corning intends to begin commercial shipments of 8G glass substrates from its Kakegawa (Shizuoka Prefecture, Japan) plant in the third quarter of 2006.
If your weevil problem is limited to the outside of your home, perhaps among the plants in your garden, you could consider this product as one of the best repellents to eliminate boll weevil. One of the weaknesses weevils have is that they cant fly. To get from one plant to another, and to invade your home or garden in the first place, they need to crawl. So if you can isolate the plants you want to protect, chances are good that you will be able to keep them free of the damage that weevils can cause.
Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides[4] Check out refs about bacteria that bioprecipitate heavy metal ...
Apple on Thursday revealed the next step in Retina display technology by packing what it calls the worlds highest resolution display into its popular all-in-one desktop.
"Cell surface glypicans are low-affinity endostatin receptors" (PDF). Molecular Cell. 7 (4): 811-22. doi:10.1016/S1097-2765(01) ... "Use of a phage display technique to identify potential osteoblast binding sites within osteoclast lacunae". Journal of Bone and ... Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable ... These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end ...
This was the first evidence of using bacterial surface display techniques to express proteins on the surface of cells, without ... Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro ... Bacterial display can be used to find peptides which bind to specific cells e.g. breast cancer cells or stem cells. Displayed ... Bacterial display combined with FACS also has the advantage that it is a real-time technique. Cell display systems were first ...
Morton, PE; Parsons, M (Jul-Aug 2011). "Dissecting cell adhesion architecture using advanced imaging techniques". Cell Adhesion ... Podosomes are conical, actin-rich structures found on the outer surface of the plasma membrane of animal cells. Their size ... but new advances in bioimaging techniques have altered that perception and show the ring complex to display a polygonal form. ... vascular smooth muscle cells, endothelial cells, and certain immune cells like macrophages and dendritic cells. A podosome ...
Surface texturing is one of techniques used to reduce optical losses to maximize light absorbed. Currently, surface texturing ... Solar panels using mono-Si cells display a distinctive pattern of small white diamonds. Epitaxial wafers of crystalline silicon ... Other possible solar cell types are organic solar cells, dye sensitized solar cells, perovskite solar cells, quantum dot solar ... the cell surface changes from reflective to adaptive, allowing the light to penetrate the cell. The other parts of the cell ...
Surface texturing is one of techniques used to reduce optical losses to maximize light absorbed. Currently, surface texturing ... Solar panels using mono-Si cells display a distinctive pattern of small white diamonds. ... Perovskite solar cells[edit]. Main article: Perovskite solar cell. Perovskite solar cells are solar cells that include a ... Main articles: Organic solar cell and Polymer solar cell. Organic solar cells and polymer solar cells are built from thin films ...
... (or yeast surface display) is a protein engineering technique that uses the expression of recombinant proteins ... The Aga2p protein is naturally used by yeast to mediate cell-cell contacts during yeast cell mating. As such, display of a ... ribosome display, bacterial display, and mRNA display. Gai, S Annie; Wittrup, K Dane (2007). "Yeast surface display for protein ... The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. The technology was sold to ...
... is a powerful technique for studying cell populations in live animals, such as mice. Different types of cells (e.g. bone marrow ... This allows luciferase to detect the efflux of ATP from the cell and will effectively display the real-time release of ATP ... Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E (May 2006). "Cell-surface-localized ATP detection with ... Biotinylation will immobilize luciferase on the cell-surface by binding to a streptavidin-biotin complex. ...
... while a C-buffer requires polygons to be displayed from the nearest to the furthest. Because the C-buffer technique does not ... Portal rendering divides a scene into cells/sectors (rooms) and portals (doors), and computes which sectors are visible by ... In 3D computer graphics, hidden-surface determination (also known as shown-surface determination, hidden-surface removal (HSR ... is the process of identifying what surfaces and parts of surfaces can be seen from a particular viewing angle. A hidden-surface ...
... surface-driven reorientation effects in liquid crystal cells with photosensitive aligning layer. Since 1995, Yuriy's main ... surface mediated photorefraction in liquid crystals, developing rollable bistable plastic liquid crystal displays, studying ... the PhD thesis Yuriy has developed a method to measure the liquid crystal anchoring energy using light scattering technique. In ... finding surface bistability in liquid crystals sandwiched between photo-aligning surfaces. Odulov, S. G., Reznikov, Y. A., ...
... so that they are displayed on the surface of the viral particle. The protein displayed corresponds to the genetic sequence ... Recently, phage display has also been used in the context of cancer treatments - such as the adoptive cell transfer approach. ... Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... Competing methods for in vitro protein evolution include yeast display, bacterial display, ribosome display, and mRNA display. ...
Nerve cells function through processes at the cell surface, with phenomena that are enhanced by largely increasing the surface ... Decalcomania, a technique used by artists such as Max Ernst, can produce fractal-like patterns. It involves pressing paint ... Also, these may include calculation or display artifacts which are not characteristics of true fractals. Modeled fractals may ... As a consequence nerve cells often are found to form into fractal patterns. These processes are crucial in cell physiology and ...
... they press against the cell walls of the phytobiont's root cells. Often the fungal and plant cell walls become almost ... With the advent of more precise genetic techniques, these traits of the mantle are often used in tandem with molecular analyses ... Much as the Hartig net and mantle, composite hyphal organs can display a wide range of structural difference. Some rhizomorphs ... However, this is largely absent in the cortical type, suggesting different strategies of increasing surface contact among ...
The surfaces of flowers can also create a diffraction, but the cell structures in plants are usually too irregular to produce ... This can be done for LCD or LED displays of smart phones as well. Because such displays are usually protected just by ... This technique is called blazing. The incident angle and wavelength for which the diffraction is most efficient (the ratio of ... The structure of a DVD is optically similar, although it may have more than one pitted surface, and all pitted surfaces are ...
On its surface the cell displays the foreign antigen with both histocompatibility complex (MHC) classes I and class II ... Several other techniques have been documented in the intervening years. Injection in saline is normally conducted ... The type of T-cell help raised is determined by the differentiated state of antigen presenting cells. Dendritic cells can ... they are recognized as foreign and when they are processed by the host cells and displayed on their surface, the immune system ...
... and surface micro-machining is used to produce thin-film transistors on large area glass substrates for flat panel displays. ... This technology can also be used for the manufacture of thin film solar cells, which can be deposited on glass, polyethylene ... Modern integrated circuit fabrication uses this technique and can use as many as 100 layers. Micro-machining is a younger ... Surface Micro-machining can be seen in action in the following MEMS (Microelectromechanical) products: Surface Micro-machined ...
The pc-AFM technique was also reported to detect local surface oxidation at a vertical resolution of 80 nm. The instrumentation ... Missing or empty ,title= (help) G.A. Chamberlain (1983). "Organic solar cells: A review". Sol. Cells. 8: 47. doi:10.1016/0379- ... The resulting image displays the local variations in morphology with the current density measurements superimposed onto of them ... Conductive atomic force microscopy (c-AFM) is one such modification technique. The c-AFM technique operates by measuring ...
B1 B cells characteristically express high levels of surface IgM (sIgM), demonstrable CD11b, and low levels of surface IgD ( ... B1 B cells self-renew and spontaneously secrete IgM and IgG3 serum antibodies. These natural serum antibodies display extensive ... from a mouse by injecting cell medium or PBS into the peritoneal cavity of the mouse and then draining it off via a technique ... There are two types of B1 cells subsets, B1a cells and B1b cells. B1b cells have been shown to be capable of memory responses. ...
... which causes an irregularity in the brain surface. The cerebral cortex, which in normal patients is six cell layers thick, is ... The resolution displays the multiple folds within the cortical area, which is continuous with the neuropathology of an infected ... The important aspect to realize is PMG affects each patient differently and treatment options and mitigation techniques will ... specifically cell division, cell migration or neurite growth. Some mutations that affect the role of microtubules and are ...
Rubbing techniques are widely used in mass production of liquid crystal displays and small laboratories as well. Due to the ... V.G. Chigrinov; V.M. Kozenkov, V.Y. Reshetnyak, Yu.A. Reznikov, M. Schadt, K. Schmitt; [1], "Liquid crystal display cell", ... Gibbons, Wayne M.; Shannon, Paul J.; Sun, Shao-Tang; Swetlin, Brian J. (1991). "Surface-mediated alignment of nematic liquid ... Photoalignment is a technique for orienting liquid crystals to desired alignment by exposure to polarized light and a photo ...
On microscopic examination, the tumor usually displays sheets of atypical cells with some resemblance to the cells of the ... Minimally invasive surgical techniques remain controversial due to the absence of long-term data, with a particular concern for ... Grossly, ACCs are often large, with a tan-yellow cut surface, and areas of hemorrhage and necrosis. ... The widely used angiotensin-II-responsive steroid-producing cell line H295R was originally isolated from a tumor diagnosed as ...
Microbeads display a large surface area per volume. This together with uniformity of size and shape provides for very good ... Kemshead, JT, Ugelstad, J (1985). "Magnetic separation techniques:their application to medicine", Mol Cell Biochem, 67(1):11-8 ... Bio-reactive molecules can be absorbed or coupled to their surface, and used to separate biological materials such as cells, ... Microbeads are used for cell isolation and cell expansion. Proteins and protein complexes can be separated, e.g. in ...
These 3D surface objects are also often arranged as "sets". Vaa3D can display multiple sets of any of these surface objects, ... The Virtual Finger technique maps the identified 2D user input via 2D display devices, such as a computer screen, back to the ... and fast cell counting in specific brain areas. Vaa3D also provides methods to annotate these cells and identify their names. ... Irregular surface objects: Each 3D surface has irregular shape and is modeled using complicated surface mesh. ...
... is more versatile than traditional printing techniques. The surface does not have to be printed under pressure ... This makes it useful for some of the techniques of printing solar cells, electronics etc. ... Graphic screenprinting is widely used today to create mass or large batch produced graphics, such as posters or display stands ... Subsequently, aluminum paste is dispensed over the whole surface of the back for passivation and surface reflection. One of the ...
Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ... While this technique utilizes only one antibody and therefore is simple and rapid, the sensitivity is lower due to little ... The Human Protein Atlas displays a map of protein expression in normal human organs and tissues. The combination of ... CD117 (KIT): for gastrointestinal stromal tumors (GIST) and mast cell tumors. CD10 (CALLA): for renal cell carcinoma and acute ...
The various leveled surface of lotus leaves is made out of convex cells (looks like bumps) and a much smaller layer of waxy ... CNT nanoforests can be made using chemical vapor deposition techniques. CNT's can be applied on a surface to modify its water ... Sun et al., have found that CNTs aligned vertically with a medium spacing display the best hydrophobic properties. Small and ... Ratio of surface area of rough surface to the surface area of a flat projection of the same surface For more complex systems ...
Ephrins: Ephrins are cell surface molecules that activate Eph receptors on the surface of other cells. This interaction can be ... In addition, severed commissural axons in chicks, display the capability of translating and expressing Eph-A2 receptor during ... Contemporary diffusion-weighted MRI techniques may also uncover the macroscopical process of axonal development. The connectome ... and activate complexes of cell-surface receptors called Plexins and Neuropilins. Cell adhesion molecules (CAMs): Integral ...
A plasma display panel (PDP) is a type of flat panel display common to large TV displays (37in. [940 mm]). Many tiny cells ... Plasma does not seem very suitable as a display technique for a digital newspaper to read. Electrowetting is the modification ... of the wetting properties of a hydrophobic surface with an applied electric field. Electrowetting is now used in a wide range ... A significant benefit of OLED displays over traditional liquid crystal displays (LCDs) is that OLEDs do not require a backlight ...
Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ... Display techniques. *Bacterial display. *mRNA display. *Phage display. *Ribosome display. *Yeast display ... CD117 (KIT): for gastrointestinal stromal tumors (GIST) and mast cell tumors. *CD10 (CALLA): for renal cell carcinoma and acute ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...
Surface-related effects can also have a large influence on luminescence color and efficiency. Surface ligands on nanocrystals ... UCNP-based systems can couple both light-based techniques and current-based techniques. This optical stimulation of ... the UCNPs target tumor cells and the plasmonic nanoparticles generate heat to kill cancer cells. [Field] nanoparticles generate ... Flexible, transparent displays have also been fabricated using UCNPs. New security inks which incorporate lanthanide doped ...
Less subject to surface artifacts, such as eye and facial movements. *Capable of at-home training due to smaller, more portable ... Photoelectric cells in a spectrophotometer device worn on the forehead measure the amount of each wavelength of light reflected ... Since then, both techniques have been applied to numerous disorders of frontal and prefrontal lobe function. Sherrill, R. (2004 ... The ratio of red/infrared light refraction is displayed as a visual signal on a computer monitor and may also be translated ...
The brain detects insulin in the blood, which indicates that nutrients are being absorbed by cells and a person is getting full ... Some mammals are omnivores and display varying degrees of carnivory and herbivory, generally leaning in favor of one more than ... Among other practices, fasting, dieting, and vegetarianism are all techniques employed by individuals and encouraged by ... Since small mammals have a high ratio of heat-losing surface area to heat-generating volume, they tend to have high energy ...
Their life cycle is thought to begin with a virion attaching to specific cell-surface receptors such as C-type lectins, DC-SIGN ... The first person infected with the disease was the village school's headmaster Mabalo Lokela, who began displaying symptoms on ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ... dendritic cells and other cells including liver cells, fibroblasts, and adrenal gland cells.[93] Viral replication triggers ...
... and surface-mount, also known as surface-mount device (SMD). The ball grid array (BGA) is the latest surface-mount package ( ... Other packaging techniques for discrete transistors as chips include Direct Chip Attach (DCA) and Chip On Board (COB).[96] ... Low operating voltages compatible with batteries of only a few cells.. *Circuits with greater energy efficiency are usually ... Flexible transistors are useful in some kinds of flexible displays and other flexible electronics. ...
Polymorphonuclear cells also infiltrate the epithelium, and chronic inflammatory cells infiltrate the lamina propria. Atrophic ... Antifungal medication can also be applied to the fitting surface of the denture before it is put back in the mouth. Other ... Oral rinse technique can distinguish between commensal candidal carriage and candidiasis. If candidal leukoplakia is suspected ... Most often, affected individuals display one clear type or another, but sometimes there can be more than one clinical variant ...
The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ... turns bright brownish red with oval white spots all over in a high contrast display.[97] Displays are often reinforced by ... The interior surfaces of the arms are covered with circular, adhesive suckers. The suckers allow the octopus to anchor itself ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ...
Potassium is the major cation (positive ion) inside animal cells,[223] while sodium is the major cation outside animal cells.[ ... When the repulsive forces within the liquid metal surface exceeds the forces of the surface tension, it vigorously explodes.[ ... thallium also displays the oxidation state +3,[10]:28 which no known alkali metal displays[10]:28 (although ununennium, the ... Angelici, R. J. (1999). Synthesis and Technique in Inorganic Chemistry. Mill Valley, CA: University Science Books. ISBN 978-0- ...
Information Display. Society for Information Display. 2006.. *^ Krepelka, J. (1992). "Maximally flat antireflection coatings" ( ... An antireflective or anti-reflection (AR) coating is a type of optical coating applied to the surface of lenses and other ... Other techniques use varying thicknesses of the coatings. By using two or more layers, each of a material chosen to give the ... and antireflective coatings on solar cells.[1] ... A circular polarizer laminated to a surface can be used to ...
The focal length of a lens is determined by its refractive index n and the radii of curvature R1 and R2 of its surfaces. The ... In this technique the objective is dipped into a drop of high refractive index immersion oil on the sample under study.[38]:14 ... due to the unique chatoyance each individual stone displays. A refractometer is the instrument used to measure refractive index ... A differential interference contrast microscopy image of yeast cells. Unstained biological structures appear mostly transparent ...
The surface of conventional activated carbon is reactive, capable of oxidation by atmospheric oxygen and oxygen plasma[26][27][ ... Other regeneration techniques[edit]. Current concerns with the high energy/cost nature of thermal regeneration of activated ... Individual particles are intensely convoluted and display various kinds of porosity; there may be many areas where flat ... to higher temperatures and either combusted to do work or in the case of hydrogen gas extracted for use in a hydrogen fuel cell ...
Electrolysis cells can be either open cell or closed cell. In open cell systems, the electrolysis products, which are gaseous, ... Fleischmann and Pons and co-workers met with Jones and co-workers on occasion in Utah to share research and techniques. During ... The lawyer later apologized; Fleischmann defended the threat as a legitimate reaction to alleged bias displayed by cold-fusion ... Although details have not surfaced, it appears that the University of Utah forced the 23 March 1989 Fleischmann and Pons ...
Odontocetes, such as the sperm whale, possess teeth with cementum cells overlying dentine cells. Unlike human teeth, which are ... The whale faeces are liquid and instead of sinking, they stay at the surface where phytoplankton feed off it.[83][84][85] ... They use a suction technique, aided by a pair of grooves on the underside of their head, not unlike the throat pleats on the ... Humpback whales, among others, are also known to display this behaviour.[68] ...
SiC can react with the surface oxide layer in order to provide diboride surfaces with higher energy: adding 5-30 vol% SiC has ... These techniques rely on metal halide and boron halide precursors (such as TiCl4 and BCl3) in the gaseous phase and use H2 as a ... Both effects reduce the overall bonding strength in the unit cell and therefore the enthalpy of formation and melting point. ... ZrB2/60%SiC composites have been used as novel conducting ceramic heaters which display high oxidation resistance and melting ...
When light passes from air or a vacuum into a transparent crystal, some of it is reflected at the surface and some refracted. ... It is represented by a lattice of points which repeats a basic pattern, called a unit cell, in three dimensions. The lattice ... More recently, driven by advances in experimental technique (such as neutron diffraction) and available computational power, ... display perhaps more of an overlap with materials science than any other discipline. ...
This allows IBM Domino applications to be better surfaced to browser clients, though the UX and business logic must be ... Properties dialog boxes also automatically recognize the type of text selected and display appropriate selections (for instance ... "Techniques to Not Send a Return Receipt When Viewing Mail".. *^ "KillNotes".. *^ Official history of Lotus Notes IBM ... The IBM Domino 9 Social Edition included the IBM Notes Browser Plugin, which would surface Notes applications through a ...
When the bladder is fully distended the superficial cells become squamous (flat) and the stratification of the cuboidal cells ... The technique can help children to urinate discreetly inside cars and in other places without being seen by others.[30] ... "raised-leg display",[89][90][91][91][92] "shadow-urination",[93] or "pseudo-urination".[94] They typically mark their territory ... Domestic dogs mark their territories by urinating on vertical surfaces (usually at nose level), sometimes marking over the ...
That allows the endospore to show up as red, while the rest of the cell stains blue. Another staining technique for endospores ... Bacillus subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of ... While the rest of a bacterial cell may stain, the endospore is left colourless. To combat this, a special stain technique ... A stained preparation of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red ...
If we consider a cell of the lattice formed, we can see that it is a rhombus with the four sides equal to d = p/sin α; (we have ... In the liquid crystal display industry, moiré is often referred to by the Japanese word mura,[citation needed] which roughly ... This technique is attractive because the scale of the moiré pattern is much larger than the deflection that causes it, making ... This can for example be due to surface reconstruction of the crystal, or when a thin layer of a second crystal is on the ...
Other techniques used by blind people to assist them in daily activities include: *Adaptations of coins and banknotes so that ... For example, the Canadian currency tactile feature is a system of raised dots in one corner, based on Braille cells but not ... Access technology such as screen readers, screen magnifiers and refreshable Braille displays enable the blind to use mainstream ... braille is a form of communication in which people use their fingers to feel elevated bumps on a surface and can understand ...
Aish Technologies makes console (display) systems for the Royal Navy off B3068 in Alderney. ... below the surface.[48] The coins were excavated by archaeologists from the Portable Antiquities Scheme.[49] ... developing the technique in the early 1980s. Edwards Sports Products of Bridport, owned by Broxap of Staffordshire, make ... Nearby on Lydiard Fields in Lydiard Tregoze is Johnson Matthey Fuel Cells, which in 2002 was the world's first production site ...
Surface hypoplasias record stressors occurring from about one to seven years, or up to 13 years if the third molar is included ... That as the body attempts to compensate for low iron levels by increasing red blood cell production in the young, sieve-like ... No permission was ever granted from surviving family for study and display. Recently, federal laws such as NAGPRA (Native ... but combines the standard forensic techniques of describing stature, sex and age with investigations of demography and ...
... surface layer homology domains, and surface layer-cell wall interactions". J Struct Biol. 124 (2-3): 276-302. doi:10.1006/jsbi. ... Theron, J.; Cloete, T.E. (2000). "Molecular techniques for determining microbial diversity and community structure in natural ... acidophilic archaea display promise for the extraction of metals from ores, including gold, cobalt and copper.[207] ... Further information: Cell wall § Archaeal cell walls. Most archaea (but not Thermoplasma and Ferroplasma) possess a cell wall.[ ...
... and other stem cells) revealed many gene promoters carrying both H3K4Me3 and H3K27Me3, in other words these promoters display ... Using an electron paramagnetic resonance spin-labeling technique, British researchers measured the distances between the spools ... Tropberger P, Schneider R (Jun 2013). "Scratching the (lateral) surface of chromatin regulation by histone modifications". ... SLBP levels are controlled by cell-cycle proteins, causing SLBP to accumulate as cells enter S phase and degrade as cells leave ...
Käss, W. Tracing Technique in Geohydrology. Rotterdam: Balkema.. *^ The Story Behind Dyeing the River Green. Greenchicagoriver. ... In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence ... This application is common in yeast display. Fluorescein can also be conjugated to nucleoside triphosphates and incorporated ... used as a rather conservative flow tracer in hydrological tracer tests to help in understanding of water flow of both surface ...
Stem cells may be of interest in transitional tissue welding.[citation needed] ... The lead removal technique will vary depending on the surgeon's estimation of the probability that simple traction will suffice ... Some devices have the availability to display intracardiac electrograms of the onset of the event as well as the event itself. ... connected to electrode leads which passed through the skin of the patient to terminate in electrodes attached to the surface of ...
Hydrogen fuel cells background information Fuel cells operate similar to a battery in that electricity is harnessed from ... Further, it was reported that "the technique is not difficult" and that "there is so much coffee around that several hundred ... Once the oil is liberated from the shoreline, the oil-biodiesel mixture is manually removed from the water surface with ... Biodiesel has displayed its capacity to significantly dissolve crude oil, depending on the source of the fatty acids. In a ...
A cell membrane isolates the inside of the cell from its environment. Neurons do not communicate via direct cytoplasm to ... New techniques are however rapidly emerging. Search on "Single neuron imaging" and see related topics: Biological neuron model ... Electrocorticography intracranial EEG, the practice of using electrodes placed directly on the exposed surface of the brain to ... Analysis of Functional NeuroImages an open-source environment for processing and displaying functional MRI data ...
British developmental biologist noted for her contributions to stem cell research and transgenic technology and techniques. She ... These questions seem different on the surface, but all of them share one quality that makes them impossible to answer: all of ... activities consist of the coming together of large groups of people to perform repeated and prolonged submissive displays to ... of cells in the cell cycle.[253]. *Mark Oliphant (1901-2000): Australian physicist and humanitarian. He played a fundamental ...
"Cell. 138 (4): 774-86. doi:10.1016/j.cell.2009.07.038. PMC 3210731. PMID 19703402.. ... proteins that function as dimers may form a heterodimer composed of one protein from each alternate gene and may display ... the fitness where all genotypes are arranged in 2D space and the fitness of each genotype is represented by height on a surface ... "iLOCi: a SNP interaction prioritization technique for detecting epistasis in genome-wide association studies". BMC Genomics ...
As bit cell size decreases, more data can be put onto a single drive platter. In 2013, a production desktop 3 TB HDD (with four ... Therefore, the surface of the magnet is half north pole and half south pole, with the radial dividing line in the middle, ... "MJM Data Recovery Ltd: Hard Disk Bad Sector Mapping Techniques". Datarecovery.mjm.co.uk. Archived from the original on February ... These techniques store extra bits, determined by mathematical formulas, for each block of data; the extra bits allow many ...
... attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. ... Receptor proteins in nerve and muscle cells have ... Unable to display preview. Download preview PDF.. References. * ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. In: Kito S., Segawa T., ...
We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of ... Cell Surface Display Techniques * Enzyme Activation / drug effects * Enzyme Inhibitors / pharmacology* * Humans ... We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of ...
It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. Peptides that ... Cell Surface Display Techniques * Hydrogen Bonding * Models, Molecular* * Molecular Dynamics Simulation * Mutation / genetics ... bind to NRP-1 and are internalized into the cell. We used peptide phage display binding assays and molecular dynamics (MD) ... It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. Peptides that ...
1. phage display 2. ribosome display 3. mRNA-peptide fusion 4. plasmid display 5. cell-surface display 6. genetics 7. n-hybrid ... There are a number of commercially available kits for error-prone PCR (link to Stratagene). Similar techniques also use ... Screening techniques. List of high-throughput assays for protein function ... There are several common techniques used to construct libraries of functional variants ...
GM7373 cells were cultured in flasks on a shaker that rhythmically tilted a thin layer of medium across the apical cell surface ... Endothelial Cell Volume and Stiffness Measurements.. Volume of living GM373 cells was determined with AFM techniques as ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Increasing Potassium Swells Endothelial Cells.. AFM imaging of living endothelial cells reveals that cell height and cell ...
After engagement of the cell surface with the particle tip, the cantilever will be bent as the cell is indented. A laser beam ( ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Indentation technique using AFM. (A) Cantilever with particle tip. (B) Principle of the stiffness measurement using an AFM. (C ... Time course of cortical cell stiffness measurements (means of 4 cells) in response to trypsin. The increase of cortical cell ...
Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. Sci. Signal. 7, rs4 ... Introducing these constructs into inert cell lines, such as HeLa epithelial cells and Cos-7 fibroblast-like cells, these cells ... exposing the C2 domain on the cell surface so that it can bind to PS on the surface of the target apoptotic cell. Simultaneous ... Wang et al. showed that Rac activation in a single cell directed the migration of border cells, a collection of 6 to 10 cells ...
25028719 - Rapidly rendering cells phagocytic through a cell surface display technique and concurr.... 20945119 - Artesunate ... of cells in the chondrocyte-like cell aggregates were positive for Scx, whereas only 3.4% of cells in the fibroblast-like cell ... Cell proliferation Ki-67 is a cell-proliferation marker [5]. In normal ACLs, 27.2% ± 4.1% of ligament cells were Ki-67 positive ... Cell density and cell arrangements The cell density in histologically normal ACLs from young donors (,45 years old) with normal ...
Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ...
... technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells ... Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...
Cell Surface Display Techniques ... Using phage display techniques, a high-affinity monoclonal ... and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo. ...
... of cell-based selection techniques has allowed selection against surface proteins in their native configuration on the cell ... All cell surface proteins cycle intracellularly to some extent, and many surface receptors are actively internalized in ... Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. ... Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking ...
Since the introduction of the yeast display platform, this method has increasingly gained popularity for the discovery and ... Overall, this chapter underlines the importance of the versatile yeast display technique for the optimization of the novel Fcab ... Sádio F., Stadlmayr G., Stadlbauer K., Rüker F., Wozniak-Knopp G. (2019) Yeast Surface Display and Cell Sorting of Antigen- ... Chao G, Lau WL, Hackel BJ et al (2006) Isolating and engineering human antibodies using yeast surface display. Nat Protoc 1(2): ...
Cells display dozens or even hundreds of molecules at their surface, that receive incoming signals and activate one or more ... This makes it difficult to study the nodes where pathways interact using traditional biochemical techniques. ... The challenge to cell biologists is to discover how the cells are sorting, editing, and refining this information at the next ... cell orientation, and haptotaxis. Cell Signal. S0898-6568(14)00379-9. doi: 10.1016/j.cellsig.2014.11.025. -full text ...
Cell surface display allows peptides or proteins to be expressed on the cell exterior as fusions to natural host anchoring ... Electrostatically Actuated Microvalves Fabricated with Soft-Lithographic Techniques for Integrated Microfluidics  Tice, Joshua ... Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. Tight regulation of cadherin adhesion plays critical ... Intracellular redox environment, the relative amount of oxidized and reduced chemical species within a cell, is important in ...
To display proteins on the cell surface of S. cerevisiae, molecular information of the native cell wall protein, α-agglutinin, ... This anchoring signal was combined with the signal of the secreted enzymes by genetic engineering techniques. Actually, we ... a cell surface glycoprotein involved in cell-cell interactions during mating. Mol. Cell. Biol. 9 1989 3155 3165 ... The display of CMCase and β-glucosidase proteins on the cell surface was confirmed by immunofluorescence microscopy. The cells ...
We discuss the strengths and pitfalls of different techniques for anti-neuronal antibody detection: 1) Immunohistochemistry and ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... The detection of neuronal surface autoantibodies in patients serum or CSF therefore has serious consequences for the patients ...
Synthetic nanopillars resembling the wing surface of certain insects display antibacterial activities. Here, Jenkins et al. ... The nanopillars induce deformation and penetration of the Gram-positive and Gram-negative bacterial cell envelope, but do not ... They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of ... This has inspired the design of antibacterial implant surfaces with insect-wing mimetic nanopillars made of synthetic materials ...
... and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. The ... The present invention relates to tumor tropic stem cells, ... terms may refer to the presence or display of a cell-surface ... Local intravascular administration by means of a catheter is a common technique in medical practice. For example, catheters as ... or any method which selectively binds the specific cell surface molecules. Alternatively, the method may use the cell surface ...
ROLs would be stabilized by the cell wall components and that ROL activity could be easily improved by display techniques. The ... of cells was obtained and used as a whole-cell catalyst. The amount of ROL displayed on the yeast cell surface was estimated to ... We used a displayed ROL and mutants of this enzyme immobilized on the yeast cell surface in organic solvents as whole-cell ... Yeast cell-surface display-applications of molecular display. Appl. Microbiol. Biotechnol.64:28-40. ...
Fn14, a cell surface receptor overexpressed in many cancers including pancreatic and triple-negative breast cancers, was ... and mirror-image phage display (d-peptide) techniques, respectively. The d-peptide ligand successfully mediated targeted drug ... Although excessive cell damage results in passive necrosis, cells can be triggered to engage molecular programs that result in ... Although a number of cell types are able to produce TNF, the ability of CD8+ T cells to produce TNF following viral infection ...
... video signal and wherein the passive display is capable of displaying a preset range of gray levels and presenting a displayed ... a light source to provide illumination of the passive display; a video signal input, wherein in response to a video signal the ... and wherein the intensity of light provided by the light source illuminating the display is controlled based on the ... passive display modulates light from the light source to provide an image, ...
... present in the context of HLA-A2 on the cell surface. We demonstrate that scFvs identified through our technique can be ... it is also displayed on the surface of the host cell. This technology allows us to combine the advantages of virus panning and ... We established a generic method for the isolation of shark-derived binding domains (sharkbodies) by yeast surface display ... Phage display to identify novel biomarkers of disease. *Development of phage display derived peptides for non-invasive in vivo ...
... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... The BACE-1 inhibitors prepared displayed IC50 values in the range of 4-45 nM, where the most potent compounds featured small P1 ... Wilkinsons catalyst [RhCl(PPh3)3] was heterogenized on common silica by the use of a grafting/anchoring technique. The ... In a preceding study we have described the development of a new hydroxyethylene (HE) core motif displaying P1 aryloxymethyl and ...
... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... Here, we show that biosynthetic enzymes can display unexpected promiscuity, with human glycosyltransferase pp-a-GanT2 able to ... TEM revealed that after several catalytic runs many of the Pd nanoparticles (NPs) had migrated to the external surface of the ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...
"Cell surface glypicans are low-affinity endostatin receptors" (PDF). Molecular Cell. 7 (4): 811-22. doi:10.1016/S1097-2765(01) ... "Use of a phage display technique to identify potential osteoblast binding sites within osteoclast lacunae". Journal of Bone and ... Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable ... These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3 end ...
These techniques revealed not only the ganglion cell impulses, but also the slow changes in ganglion cell membrane potential ... The arrangement of ganglion cells on the retinal surface is not spatially random. Each cell type is distributed in an ordered ... In the right column gain has been increased to better display the lower amplitude rod signals. Stimulus brightness is given in ... Y cells are called brisk transient cells, and X cells are called brisk sustained cells (Cleland and Levick, 1974a). Y cells ...
It is a technique often used for the selection of phage displays during antibody drug development. Biotinylated proteins can be ... Biotinylated proteins can be used along fluorophore-tagged SA to detect/isolate cells expressing particular surface markers. ... 5 - Cell based assay - Recombinant human TNF-alpha (Cat. No. TNA-H82E3) induces cytotoxicity effect on the WEH1-13VAR cells in ... The batch-to-batch conformance is also good as displayed in cell based assay (Fig. 7). ...
... or both on their outer membranes or cell surfaces.. Our efforts focus on the discovery of novel carbohydrate-binding proteins ... and trafficking of tumor cells during metastasis. Not to be forgotten all microbes and many viruses display unique glycan ... This is accomplished using glycan profiling and biophysical techniques, evaluation of antimicrobial or antiviral activities, ... The initial step of HIV infection involves stepwise binding of the surface envelope glycoproteins, gp120/gp41, to cellular ...
Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity ... Visit for more related articles at Journal of Analytical & Bioanalytical Techniques View PDF Download PDF ... due to sheer number of cells to sort), or how well the peptide is displayed on the cell surface. In this test case, both ... for cell sorting using yeast surface display [28-31], as well as for sorting tumor epithelial cells for downstream screening of ...
  • Receptor proteins in nerve and muscle cells have attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. (springer.com)
  • SELEX has traditionally been performed using purified proteins, and cell surface receptors may be challenging to purify in their properly folded and modified conformations. (mdpi.com)
  • However, improvements in recombinant fusion protein technology have increased the availability of receptor extracellular domains as purified protein targets, and the development of cell-based selection techniques has allowed selection against surface proteins in their native configuration on the cell surface. (mdpi.com)
  • All cell surface proteins cycle intracellularly to some extent, and many surface receptors are actively internalized in response to ligand binding. (mdpi.com)
  • Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. (bgsu.edu)
  • The display of CMCase and β-glucosidase proteins on the cell surface was confirmed by immunofluorescence microscopy. (asm.org)
  • To display proteins on the cell surface of S. cerevisiae , molecular information of the native cell wall protein, α-agglutinin, was utilized. (asm.org)
  • α-Agglutinin is a cell surface adhesion molecule involved in mating ( 11 ) and has a glycosylphosphatidylinositol anchor attachment signal, which is engaged in anchoring of cell wall proteins ( 10 , 27 ). (asm.org)
  • They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of oxidative stress proteins. (nature.com)
  • Proteins are immobilized by using an outer shell cell wall protein, the C-terminal half of α-agglutinin ( 10 , 26 ). (asm.org)
  • Biotinylated proteins can be used along fluorophore-tagged SA to detect/isolate cells expressing particular surface markers. (2bscientific.com)
  • Biotinylated proteins can be used with SA-coated magnetic beads/surface in biopanning with higher coating density and uniformed antigen presentation. (2bscientific.com)
  • Also, the biotinylated proteins are often used in cell based assay, such as cytotoxicity assay and evaluation of CAR expression (Fig. 5 & Fig. 10). (2bscientific.com)
  • Not to be forgotten all microbes and many viruses display unique glycan structures, carbohydrate-binding proteins, or both on their outer membranes or cell surfaces. (nih.gov)
  • sodium/hydrogen exchangers (NHEs) are a group of membrane proteins that exchange extracellular Na + for intracellular H + , and they display broad tissue distribution. (physiology.org)
  • GAGs interact with proteins displayed on the surface of cells to control signalling events. (europa.eu)
  • However, study of these interactions is very challenging as the GAGs sit on the surface of proteins and not in deep binding pockets, and are attracted through a series of weak interactions. (europa.eu)
  • These techniques have allowed the Fellow to look at footprinting of specific GAGs onto the surface of proteins such as factor H module 7 (fHapp.7) which is thought to play an important role in the development of age-related macular degeneration (one of the leading causes of blindness in the Western world). (europa.eu)
  • Using molecular biological techniques, Sutter's team have altered the structure of the MVA genome in such a way that genetic information coding for proteins characteristic of other viral pathogens can be spliced into it. (uni-muenchen.de)
  • Using this screening method, randomized libraries of peptides can be generated and screened for affinity and selective molecular targeting to cell surface receptor proteins, such as the transferrin receptor ( 7, 8 ). (aacrjournals.org)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing NGF domain in the selected taxonomic class. (embl-heidelberg.de)
  • Directed evolution of proteins is a technique used to modify protein functions through "Darwinian selection. (hindawi.com)
  • Liposome-based IVC is characterized by in vitro protein synthesis from a single copy of a gene in a cell-sized unilamellar liposome and quantitative functional evaluation of the synthesized proteins. (hindawi.com)
  • Target proteins fused with a membrane protein are displayed on the cell membrane surface, screened for the desired function by exposure to a colorimetric detection reagent, and selectively sorted using a fluorescence-activated cell sorter (FACS) [ 4 , 5 ]. (hindawi.com)
  • In this technique, target proteins are fused with phage coat proteins ( g8p or g3p ) and displayed on a phage surface. (hindawi.com)
  • The first area of focus is to examine the polarized trafficking of two integral membrane proteins in epithelial cells, the transferrin receptor (TR) and the cystic fibrosis transmembrane conductance regulator (CFTR). (uab.edu)
  • One thing to consider: in the absence of aptamers that bind to the cell surface, we might as well try it with aptamers that bind proteins in vitro as done in 12000850. (openwetware.org)
  • Bacterial display can be used to find target proteins with desired properties and can be used to make affinity ligands which are cell-specific. (wikipedia.org)
  • Cell display systems were first used in 1985, when peptides were genetically fused with proteins displayed on the M13 bacteriophage. (wikipedia.org)
  • Bacteriophage display is a commonly used cell display system, although it carries limitations in the size of proteins that can be displayed. (wikipedia.org)
  • Bacterial display was then introduced in 1986, allowing the surface display of larger proteins. (wikipedia.org)
  • in 1986, when they used bacterial surface proteins OmpA and LamB to display peptides. (wikipedia.org)
  • They showed that the proteins were now subject to cleavage by proteinase K. The non-OmpA peptides inserted were therefore a target of proteinase K. Insertion of the foreign peptides did not affect bacterial cell growth. (wikipedia.org)
  • This was the first evidence of using bacterial surface display techniques to express proteins on the surface of cells, without altering the function of the cell. (wikipedia.org)
  • Cells express surface proteins which can be involved in a whole host of responses including recognition of other cells, interaction with other cells, and cell signalling. (wikipedia.org)
  • Many types of bacteria have cell surface proteins such as the enteropathogenic E. coli intimin protein which is involved in binding to host cells, or the OmpA protein of E. coli cells which is important in keeping the structure of the outer membrane. (wikipedia.org)
  • Many surface proteins are involved in bacterial cell attachment and invasion of the host cell. (wikipedia.org)
  • By using bacterial display, target proteins on the host cell can be identified. (wikipedia.org)
  • These surface proteins need to first be translocated across the bacterial cell membranes from the cytoplasm to the cell surface. (wikipedia.org)
  • The display of heterologous proteins on the bacterial cell surface normally requires the fusion of the protein with a surface protein, called a scaffold. (wikipedia.org)
  • Proteins can also be displayed on the bacterial cell surface through the use of autotransporters. (wikipedia.org)
  • There are many fimbriae on mainly Gram-negative bacteria, so displaying proteins on fimbriae is advantageous over some other surface proteins which are less numerous. (wikipedia.org)
  • In the ring complex, integrins and integrin-associated proteins serve to connect the cytoskeleton to cell surface integrins forming the outward protrusion. (wikipedia.org)
  • These finding were made possible through the application of Bayesian blinking and bleaching analytics to data gained from standard widefield microscopy using cells that expressed fluorescently tagged proteins specific to the podosome ring complex. (wikipedia.org)
  • Previous studies have revealed that the C-terminal region of the S-layer protein from Lactobacillus is responsible for the cell wall anchoring, which provide an approach for targeting heterologous proteins to the cell wall of lactic acid bacteria (LAB). (biomedcentral.com)
  • Moreover, both of the fused proteins could simultaneously bind to the surface of a single cell. (biomedcentral.com)
  • The LcsB region can be used as a functional scaffold to target the heterologous proteins to the cell surfaces of lactic acid bacteria in vitro and in vivo , and has also the potential for biotechnological application. (biomedcentral.com)
  • S-layer proteins normally contain two functional regions: the self-assembly domain and the cell wall-targeting domain. (biomedcentral.com)
  • In the S-layer proteins of lactobacilli, no S-layer homology (SLH) motif at the C-terminus, which was responsible for the non-covalent attachment of the protein subunits to the cell wall through a pyruvylated polysaccharide receptor in the cell wall [ 6 ], has been identified. (biomedcentral.com)
  • The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)
  • These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)
  • The proteins then fold under oxidizing conditions and are displayed on the cell surface or packaged into phage particles to screen for binding affinity 17,18 . (jove.com)
  • To improve the efficiency of engineering antibodies that are well folded in the cytoplasm, we previously reported the success of MAD-TRAP (membrane-anchored display for Tat-based recognition of associating proteins), a method for screening an scFv antibody library using Escherichia coli inner-membrane display 19 . (jove.com)
  • Influenza type A is the most common virus to infect humans, and in order to bind to host cells it uses two proteins on its surface: haemagluttinin and neuraminidase. (www.nhs.uk)
  • The virus is further divided into varieties depending on which types of these two proteins it has on its surface. (www.nhs.uk)
  • Once an antibody is selected, the genes for that antibody are transferred into an expression system-bacteria, yeast, or mammalian cell lines specially designed for the expression of foreign proteins. (jhsph.edu)
  • The AIDA system was then used for the expression of two Salmonella surface proteins, SefA and H:gm, with potential for use as vaccine epitopes. (diva-portal.org)
  • Since a live bacterial vaccine based on surface display of antigens has many advantages over traditional vaccines, we have studied the surface display of the SE antigenic proteins, H: gm and SefA in Escherichia coli by the beta-autotransporter system, AIDA. (diva-portal.org)
  • Surface display is a powerful technique that utilizes natural microbial functional components to express proteins or peptides on the cell exterior. (unt.edu)
  • Cross-reactive anti-heat-shock-protein antibodies and T cells can damage vascular tissues overexpressing warmth shock proteins, contributing to the development of atherosclerosis (Mandal et al. (cylch.org)
  • One of the most important mechanisms by which cells receive and decode signals from the outside world is by adding or removing phosphate from specific tyrosine amino acids in cellular proteins, which alters the functions of the modified proteins. (cancer.gov)
  • Like the PTKs, PTPs comprise a large gene family that encodes both cell surface (receptor-like) and intracellular (non-transmembrane) proteins. (cancer.gov)
  • Antibodies are the first proteins which were successfully displayed on the surface of phage by fusing the coding sequence of scFv or Fab to the coat protein. (creative-biolabs.com)
  • I think I mentioned to you already that I began to work with a colleague, Mark Willingham, studying how hormones and proteins bound to the cell surface, how they behaved on the cell surface, how they entered cells, and the pathway by which they entered cells. (nih.gov)
  • And since we were working with receptors, and receptor mediated endocytosis, we said that this vesicle that we see inside the cell that is moving around and that contains hormones and other proteins, should be called a receptosome. (nih.gov)
  • We measured the rates of internalization of different proteins through this vesicular pathway, how many different molecules could reside in the same vesicle, how many vesicles were formed per minute, diffusion rates of receptor-hormone complexes on the cell surface and the likelihood they would get into a vesicle and be internalized. (nih.gov)
  • Their novel strategy introduces specific, dynamic and reversible supramolecular functionality on the bacterial cell surface by adopting a bacterial display system that has been used before exclusively to identify high affinity peptides for various proteins. (nanowerk.com)
  • To do this, they genetically tweaked the virus to make it express certain proteins on its surface, which influence the growth of the tissue. (technologyreview.com)
  • Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell. (bvsalud.org)
  • To overcome these drawbacks, new imaging technologies have been described recently that are based on the detection of resonance energy transfer (RET) between fluorescent or luminescent proteins (FPs or LPs) ( Box 1 ) [ 5 ] and on protein fragment complementation approaches [ 6 , 7 ], both of which facilitate real-time characterization and visualization of PPIs in live cells. (pubmedcentralcanada.ca)
  • Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • Phage display technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John McCafferty, The Scripps Research Institute with Richard Lerner and Carlos Barbas and the German Cancer Research Center with Frank Breitling and Stefan Dübel for display of proteins such as antibodies for therapeutic protein engineering. (wikipedia.org)
  • Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have allowed the production of highly specific recombinant antibodies. (frontiersin.org)
  • Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, immunomodulation, and broad applications of antibodies are discussed to enhance innovative antibody production in pursuit of a healthier future for humans. (frontiersin.org)
  • Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. (mdpi.com)
  • Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. (mdpi.com)
  • Since the introduction of the yeast display platform, this method has increasingly gained popularity for the discovery and affinity maturation of antibodies and other protein scaffolds intended for antigen recognition. (springer.com)
  • Chao G, Lau WL, Hackel BJ et al (2006) Isolating and engineering human antibodies using yeast surface display. (springer.com)
  • In addition, antibodies directed at classical PNS antigens are mostly recognizing linear epitopes, whereas surface antigens contain mostly conformational epitopes. (frontiersin.org)
  • I will present VirScan, a high-throughput method to comprehensively analyze antiviral antibodies using immunoprecipitation and massively parallel DNA sequencing of a bacteriophage library displaying proteome-wide peptides from all human viruses. (pegsummit.com)
  • Once there, it can be recognized by cells of the immune system, which are stimulated to produce antibodies and T cells against it. (uni-muenchen.de)
  • In earlier assays, the researchers had confirmed the immunogenicity of their candidate vaccine in cell culture, and demonstrated that it elicits the production of protective levels of MERS-CoV-neutralizing antibodies. (uni-muenchen.de)
  • Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
  • These approaches commonly use phage display, yeast surface display, or bacterial surface display to screen large libraries of antibodies 11-13 . (jove.com)
  • As a result, antibodies isolated using these techniques will not necessarily fold well in the cytoplasm, and intracellular solubility must often be engineered separately if the antibodies will be used in intracellular applications. (jove.com)
  • Bacterial inner-membrane display relies on the twin-arginine translocation (Tat) pathway for transporting displayed antibodies, in contrast to other common display methods that use the secretory pathway. (jove.com)
  • The newspaper says a "holy grail" flu vaccine is one step closer after scientists have found antibodies (disease-fighting cells) which target a weak spot on most forms of flu virus (including the deadly form of bird flu) and prevents them from infecting cells. (www.nhs.uk)
  • In this laboratory study, researchers used genetic and biochemical techniques to select antibodies that could neutralise H5N1 infection both in mice and cell cultures outside living bodies. (www.nhs.uk)
  • As many flu subtypes have similar chemical sequences in molecules on their surface, the researchers then examined how well the antibodies would bind with other viruses in cell cultures. (www.nhs.uk)
  • Further experiments revealed that the antibodies did not prevent initial binding of the virus to blood cells, but prevented the later step when the membranes of the two cells fuse. (www.nhs.uk)
  • The manufactured antibodies bound to cells that were infected with several different viruses: three different H1 viral strains (including the H1N1 that caused the 1918 Spanish flu pandemic), an H2 virus, an H6 virus, H13 and H16, H9 from three different viral strains and one type of H11 virus. (www.nhs.uk)
  • This creates a physical linkage between each variant protein sequence and the DNA encoding it, which allows rapid partitioning based on binding affinity to a given target molecule (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called panning. (neb.com)
  • These techniques rely on rapid cloning of immunoglobulin gene segments to create libraries of antibodies with slightly different amino acid sequences from which antibodies with desired specificities can be selected. (jhsph.edu)
  • Recombinant antibodies are translated from recombinant DNA and displayed on the surfaces of cells or phage particles. (jhsph.edu)
  • In 1990 John McCafferty demonstrated that variable regions from antibodies could be displayed on the surface of a filamentous phage. (jhsph.edu)
  • Genes encoding antigen-binding variable domains of antibodies are fused to phage genes that encode coat protein, allowing for the phage coat to express, or "display", the antibody fusion protein. (jhsph.edu)
  • Display on the cell or phage surface of the cloned antibody sequence allows for rapid, high throughput selection of recombinant antibodies that bind a specific antigen. (jhsph.edu)
  • Once the rAbs are displayed, tools such as paramagnetic beads, fluorescence-activated cell sorting (FACS), and/or Enzyme-Linked Immunosorbent Assays (ELISAs) can be used to isolate individual antibodies that bind to a specific antigen target. (jhsph.edu)
  • As a consequence of advances in the approaches for analyzing humoral ( 3 ) and cellular ( 4 ) immune reactivity to cancer in the context of the autologous host, an increasing number of tumor antigens recognized by autologous CD8 + T cells ( 5 ) and/or antibodies ( 6 ) have been identified. (aacrjournals.org)
  • Recombinant antibodies (rAbs) are synthetic antibodies that are created using antibody genes made in a laboratory or taken from human cells, completely eliminating animals from the antibody-production process. (prolabmarketing.com)
  • These antibodies are translated from recombinant DNA and displayed on the surfaces of cells or phage particles. (prolabmarketing.com)
  • Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing mammalian cells, as well as to produce antibodies against the displayed antigen. (cshlpress.com)
  • Flow cytometry showed that surface EnvG bound various conformationally- and trimer-specific antibodies (Abs), and in-vitro growth assays on CD4+CCR5+ cells demonstrated EnvG functionality. (jove.com)
  • Here we describe a cell-based ELISA assay that allows studying the recognition of trimeric HIV-1 Env by monoclonal antibodies. (jove.com)
  • Following expression of HIV-1 trimeric Env at the surface of transfected cells, conformation specific anti-Env antibodies are incubated with the cells. (jove.com)
  • Thus, this technique can be established for medium to high throughput screening of antigens and antibodies, such as newly-isolated antibodies. (jove.com)
  • Researchers insert the genes for antibodies into one-celled organisms such as yeast, modified so that the antibodies made by the yeast are displayed on their surface. (harvard.edu)
  • The IPI's first major effort will be to develop open-source libraries of well-validated antibodies targeting every human protein found outside cells. (harvard.edu)
  • This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. (creative-biolabs.com)
  • Cell-associated molecules are measured with a variety of probes, most often fluorochrome-labeled monoclonal antibodies. (cancer.gov)
  • Peptides that expose the consensus sequence R/K/XXR/K at the C-terminus (C-end rule or CendR peptides) bind to NRP-1 and are internalized into the cell. (nih.gov)
  • We used peptide phage display binding assays and molecular dynamics (MD) simulations to study the potential role of the central residues of CendR peptides in binding and activation of the NRP-1 receptor. (nih.gov)
  • Here, we have developed a methodology for bacterial cell sorting using the semi-automated autoMACS® Pro Separator for the first time, and have produced a complete method for sorting of bacteria displaying 15-mer peptides on their cell surface using this device, including downstream bioinformatic analysis of candidates for binding to a target of interest. (omicsonline.org)
  • Numerous studies suggest that small peptides can efficiently bind to surface receptors ( 4 ). (aacrjournals.org)
  • Using phage display analysis, Dr. Collawn's lab has identified peptides that bind to cell surface receptors on dendritic and B cells. (uab.edu)
  • Their use is getting more popular, and display systems offer a useful way to engineer peptides and optimise their binding capabilities. (wikipedia.org)
  • At MDACC, Drs. Pasqualini and Arap used a technique called phage display to generate a vast library of specific peptides that home to both known and novel receptors. (nanowerk.com)
  • and (2) peptides were identified that specifically bind these and previously known receptors and are rapidly internalized by the cells of interest. (nanowerk.com)
  • Alvos has identified rapidly internalizing cell surface receptors for over 30 diseased and normal tissue types and peptides that efficiently target them. (nanowerk.com)
  • In vitro stimulation of peripheral blood mononuclear cells from a patient bearing SSX-4 -expressing tumor with a pool of long peptides spanning the protein sequence was used for assessment of SSX-4 -specific CD4 + T cells recognizing distinct antigenic sequences restricted by HLA class II alleles. (aacrjournals.org)
  • Peptides presented by human leukocyte antigen (HLA) molecules on the cell surface play a crucial role in adaptive immunology, mediating the communication between T cells and antigen presenting cells. (mcponline.org)
  • Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action and in cellular immunotherapy and transplantation. (mcponline.org)
  • Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action, the design of T-cell-mediated therapies such as tumor immunotherapy ( 1 ), and the treatment of hematological malignancies through a combination of hematopoietic stem cell transplantation and donor lymphocyte infusion ( 2 ). (mcponline.org)
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
  • This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
  • With cell-based selection, a specific protein target is not always chosen, but selection is performed against a target cell type with the goal of letting the aptamer choose the target. (mdpi.com)
  • 4. The isolated stem cell of claim 2, wherein said isolated stem cell exhibits a glial fibrillary acidic protein (GFAP) astrocytic precursor marker. (freepatentsonline.com)
  • 16. The method of claim 14, wherein said stem cell exhibits a glial fibrillary acidic protein (GFAP) astrocytic precursor marker. (freepatentsonline.com)
  • In this study, we evaluated applications of genetically immobilized Rhizopus oryzae lipase displayed on the cell surface of Saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with α-agglutinin. (asm.org)
  • Thus, yeast cells displaying the lipase can be used in organic solvents, and the lipase activity may be increased by a combination of protein engineering and display techniques. (asm.org)
  • Protein-carbohydrate interactions play critical roles in countless biological processes and recognition events as diverse as fertilization, leukocyte homing during the course of inflammation, and trafficking of tumor cells during metastasis. (nih.gov)
  • Immunohistochemistry staining located NHE8 protein at the plasma membrane of the epithelial cells in the conjunctiva, the cornea, and the lacrimal gland both in human and mouse. (physiology.org)
  • This will then allow the determination of the distance between the ends of the GAG chain by fluorescence (yielding vital structural information about the GAG), and also "footprinting" of the GAG upon the surface of a protein (yielding vital information about the GAG binding site on the protein). (europa.eu)
  • The modified virus is then able to synthesize the foreign protein and displays it on the surface of infected cells. (uni-muenchen.de)
  • One way to identify peptide ligands for protein targets is phage display ( 5, 6 ). (aacrjournals.org)
  • cell surface display via Ice Nucleation Protein, and phage display via M13's major coat protein. (igem.org)
  • A more radical proposal involves use M13 phage as the scaffold, and attaching enzymes by phage-display techniques to the pVIII coat protein. (igem.org)
  • Herein, we introduce a new liposome-based IVC system consisting of a liposome, the protein synthesis using recombinant elements (PURE) system and a fluorescence-activated cell sorter (FACS) used as a microcompartment, in vitro protein synthesis system, and high-throughput screen, respectively. (hindawi.com)
  • Subsequently, the protein variants are synthesized from the mutated genes using living hosts (cells) or an in vitro transcription-translation system (IVTT), and they are screened for the desired function. (hindawi.com)
  • For example, cell-surface display is an in vivo screening technique that uses a fusion protein to localize protein molecules to a cell membrane surface. (hindawi.com)
  • Phage display is another in vivo display technique that uses a phage for gene storage and protein display. (hindawi.com)
  • It is a fusion protein N - Lpp aa's 1-9 - OmpA aa's 46-159 - surface protein - C. E. coli K12 genome and BioBrick primers for the full OmpA gene (start through stop) have been ordered. (openwetware.org)
  • Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. (wikipedia.org)
  • This protein engineering technique allows us to link the function of a protein with the gene that encodes it. (wikipedia.org)
  • Competing methods for protein evolution in vitro are phage display, ribosome display, yeast display, and mRNA display. (wikipedia.org)
  • Scaffolds are used to display the heterologous protein on the bacterial cell surface. (wikipedia.org)
  • Another method of heterologous protein fusion is fusion with fimbriae/flagella, which are filamentous protrusions on the cell surface. (wikipedia.org)
  • Patients who exhibit Frank-ter Haar syndrome are known to be mutant for the podosome specific protein Tks4 and demonstrate defects in neural crest cell migration. (wikipedia.org)
  • In this study, we developed a new surface display system in lactic acid bacteria with the C-terminal region of S-layer protein SlpB of Lactobacillus crispatus K2-4-3 isolated from chicken intestine. (biomedcentral.com)
  • Surface layers (S-layers), which are composed of protein or glycoprotein subunits, are the outmost cell surface structure of many Bacteria and Archaea. (biomedcentral.com)
  • Previous attempts to produce recombinant sporozoite surface antigen ( p67) in bacterial or insect cells for vaccine purposes have not resulted in a correctly folded protein. (wikigenes.org)
  • We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
  • They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)
  • It is a selection technique in which a library of variants of a peptide or protein is expressed on the outside of a phage virion, while the genetic material encoding each variant resides on the inside. (neb.com)
  • Vasotide, they found, keeps a protein called vascular endothelial growth factor, VEGF, from binding to two different kinds of receptors on the surface of endothelial cells. (unm.edu)
  • However, while much basic research is directed towards mechanisms of surface expression using autotransporters there are few reports regarding production of surface expressed protein. (diva-portal.org)
  • Thus the aim of this work was the optimisation of the yield and productivity of surface expressed protein. (diva-portal.org)
  • Protein Z, an IgG-binding domain of Staphylococcal protein A, was used as a model protein for the investigation of which cultivation parameters influenced surface expression. (diva-portal.org)
  • The removal of proteolysis and further optimisation of the yield of surface-expressed protein are important goals of further work. (diva-portal.org)
  • Microbial cell-surface display has proven to be extremely important for numerous applications ranging from combinatorial library screening and protein engineering to bioremediation and biofuels production. (unt.edu)
  • The inherently hydrophobic surfaces of MPs complicates protein expression, purification, and characterization. (rsc.org)
  • ShuA variants were displayed on the surface of an M13 bacteriophage as fusions to the P8 coat protein. (rsc.org)
  • To counter the aggregated protein’s toxic effects, cells induce several responses, such as the ER overloading response (ERO), unfolded protein response (UPR), and autophagy.Several therapeutic methods have been proposed to remove the toxic aggregation,such as small molecule inhibition, autophagy up-regulation, RNA interference,and genomic correction. (ufl.edu)
  • Our future studies will focus on the detailed mechanisms of the protein-protein interaction and how cells respond to these two therapies. (ufl.edu)
  • M. T. Reetz,* J. Sanchis Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process ChemBioChem DOI: 10.1002/cbic.200800371 Surface Chemistry Perfect support: Microfabrication techniques to create arrays of identical liquid-crystalline areas for membrane support and protein display are demonstrated. (docme.ru)
  • In the March issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc3_10.dtl ), Shao-En Ong from the Broad Institute of MIT and Harvard ( www.broadinstitute.org/proteomics/team.html ) presents Unbiased Identification of Protein/Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics . (cshlpress.com)
  • Billions of yeast cells, each bearing a unique antibody, are then mixed with the protein of interest. (harvard.edu)
  • The ZapA cell division protein (blue) localizes to the FtsZ ring. (nih.gov)
  • Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. (creative-biolabs.com)
  • Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype. (creative-biolabs.com)
  • Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific target binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naïve targets. (creative-biolabs.com)
  • This was genetically fused to a modified outer membrane protein eCPX, which enabled it to be displayed on the outer membrane and bind CB[8]. (nanowerk.com)
  • In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. (pubmedcentralcanada.ca)
  • In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. (wikipedia.org)
  • Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. (wikipedia.org)
  • In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
  • If a "phagemid" vector is used (a simplified display construct vector) phage particles will not be released from the E. coli cells until they are infected with helper phage, which enables packaging of the phage DNA and assembly of the mature virions with the relevant protein fragment as part of their outer coat on either the minor (pIII) or major (pVIII) coat protein. (wikipedia.org)
  • By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein that binds to one of those targets on its surface will remain while others are removed by washing. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • Phage display is also a widely used method for in vitro protein evolution (also called protein engineering). (wikipedia.org)
  • Similarly, as shown in renal epithelium with scanning ion conductance microscopy ( , 12 ), aldosterone stimulates endothelial cell volume, growth, and stiffness by activating epithelial sodium channels (ENaCs) ( , 13 , , 14 ). (pnas.org)
  • Therefore, relatively few aptamers have been generated that bind cell surface receptors. (mdpi.com)
  • Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking receptor-ligand interactions. (mdpi.com)
  • Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. (mdpi.com)
  • Yeast display is particularly well suited for the selection of antigen-binding Fc fragments (Fcabs) as it allows rapid combinatorial library construction via gap repair-driven homologous recombination and an efficient display of a glycosylated Fc able to interact with Fcγ receptors. (springer.com)
  • The stem cells with tumor tropic potential are selected based on the stem cells exhibiting CXCR4 receptors or an affinity for the chemokine SDF-1. (freepatentsonline.com)
  • This information is in the form of chemical messages sensed by receptors on the ganglion cell membrane. (utah.edu)
  • The initial step of HIV infection involves stepwise binding of the surface envelope glycoproteins, gp120/gp41, to cellular receptors, CD4 and CCR5 or CXCR4. (nih.gov)
  • Dendritic cells are equipped with receptors for antigen (HIV), with cell adhesion molecules, and receptors for immunoregulatory and costimulatory signals. (natap.org)
  • They are screening for specific receptors that undergo RME in order to develop methodologies for enhancing antigen uptake and processing by antigen-presenting cells in vitro. (uab.edu)
  • Normally, when VEGF binds to one of its receptors the binding causes endothelial cells to multiply, allowing blood vessels to form. (unm.edu)
  • The glycan receptors for AB5 toxin B subunit pentamers are displayed either on glycolipids (for Ctx/LT and Stx) or on glycoproteins (for Ptx and SubAB). (functionalglycomics.org)
  • Megakaryocytic differentiation is accompanied by changes in cell morphology, notably an increase in size and the appearance of demarcation membranes, and by the sequential expression of a number of genes coding for specific cell surface markers, cytokines, and cytokine receptors. (bloodjournal.org)
  • Swiss researchers have designed a groundbreaking technique that uses artificial receptors to enhance the body's immune response to tumors. (medicalnewstoday.com)
  • In contrast, a low dose of trypsin, known to activate sodium influx through epithelial sodium channels, stiffens the submembranous cell cortex. (pnas.org)
  • Sodium/hydrogen exchanger (NHE) 8 is expressed at the apical membrane of the epithelial cells and plays important roles in neutral sodium absorption in the gastrointestinal tract and the kidney. (physiology.org)
  • We also detected the expression of downregulated-in-adenoma (DRA, a Cl − /HCO 3 − transporter) in the ocular surface epithelial cells. (physiology.org)
  • Altered ocular surface function and reduced epithelial DRA expression in NHE8−/− mice suggest that the role of NHE8 in ocular surface tissue involve in tear production and ocular epithelial protection. (physiology.org)
  • Among them, five NHEs are detected at the plasma membrane in the epithelial cells in the intestine and the kidney. (physiology.org)
  • NHE1 is detected in almost all tissues and is located at the basolateral membrane in the epithelial cells ( 39 , 42 ). (physiology.org)
  • NHE2 and NHE3 are mainly expressed at the apical membrane in the intestinal and the renal epithelial cells ( 9 , 14 , 39 ). (physiology.org)
  • NHE4 is also detected in the gastric epithelial cells and renal epithelial cells ( 39 ). (physiology.org)
  • NHE8, the latest characterized NHE isoform, is expressed at the apical membrane in the intestinal and renal epithelial cells ( 25 , 49 ). (physiology.org)
  • This mouse model mimics the majority of human BRCA1 breast cancers and exhibits significant alterations in mammary gland morphology, increased mammary epithelial cell proliferation, and upregulation of the progesterone receptor. (army.mil)
  • The investigators have demonstrated that treatment of these BRCA1/p53 conditional knockout mice with the anti-progesterone mifepristone significantly delayed the onset of mammary tumor development, reduced mammary gland morphological dysfunction, and retarded mammary epithelial cell and hyperplastic foci progression to tumors. (army.mil)
  • For example, differentiated airway epithelial cells can revert into stable and functional stem cells in vivo after the ablation of airway [24] . (wikiversity.org)
  • Chen W-T, Greve JM, Gottlieb DI, Singer SJ (1985) Immunocytochemical localization of 140 kD cell adhesion molecules in cultured chicken fibroblasts, and in chicken smooth muscle and intestinal epithelial tissues. (springer.com)
  • Goblet cells are epithelial cells that secrete mucins onto the ocular surface to maintain the wet environment for protection with tear. (postech.ac.kr)
  • A quantitative thermodynamic model proposed that the bactericidal activity of nanopatterned surfaces is directly related to the balance between adhesion energy and deformation energy. (nature.com)
  • EGFR is a tyrosine kinase cell surface receptor that regulates growth and survival including adhesion, migration, differentiation, and other cellular processes ( 3 ). (aacrjournals.org)
  • The physic-chemical properties of the MCs and bioactive coatings modulated cell adhesion, proliferation, and migratory potential in response to chemokines that regulate MSC tissue localization, like SDF-1a. (upc.edu)
  • The Adhesion of Particles Undergoing an Elastic-Plastic Impact with a Surface", J. Phys. (patentgenius.com)
  • and (3) after a period of time measuring the distance from a fixed point in the measuring chamber in a direction towards the outlet thereof over which cells remain on the said surface in the measuring chamber after the passage of fluid therethrough,the distance measurement giving a measure of the adhesion of the cells to the substratum. (patentgenius.com)
  • 4. Apparatus as claimed in claim 3, wherein the measuring chamber is of rectangular cross-section and either one or both of the opposed pair of parallel internal faces of the chamber are formed from or coated with a substratum whose adhesion tothe cells under test is to be measured. (patentgenius.com)
  • 6. Apparatus as claimed in claim 4, wherein a wall of the chamber in which cells remain after the test is calibrated so that measurement of cell adhesion can be made by direct observation. (patentgenius.com)
  • Such surfaces would allow for the simultaneous investigations of adhesion site maturation and composition and how changes in these properties can be implemented to engineer cell behavior via cell-surface interactions. (asme.org)
  • For example, nanostructures can be used to mimic patterns of surfaces found in nature to endow the synthetic surfaces with properties such as inherent colour effects, tack-free adhesion to surfaces, water-proofing and anti-reflectivity. (nanotech-now.com)
  • The discussion will be centered on pancreatic cancer as an example of the power of phage display derived peptide ligands. (pegsummit.com)
  • Sarkes DA, Dorsey BL, Finch AS, Stratis-Cullum DN (2015) Method for Discovery of Peptide Reagents Using a Commercial Magnetic Separation Platform and Bacterial Cell Surface Display Technology. (omicsonline.org)
  • Peptide candidates produced with this method contained the previously reported PA consensus WXCFTC, further validating this method and the commercially available autoMACS® platform as the first low cost, semi-automated biopanning approach for bacterial display that is widely accessible and more reliable than the MACS/FACS standard protocol. (omicsonline.org)
  • As compared with yeast and phage display, bacterial display is ideal because of the fast doubling time of bacteria, about 20 minutes for Escherichia coli ( E. coli ) [ 7 ] versus about 2 hours for Saccharomyces cerevisiae ( S. cerevisiae ) [ 8 , 9 ], and direct amplification of the bound bacterial cells containing plasmid DNA encoding the displayed peptide responsible for binding, without elution and reinfection. (omicsonline.org)
  • Using phage display techniques, Li has identified a 12-residue linear peptide sequence, GE11 (YHWYGYTPQNVI) approaching nanomolar affinity for EGFR ( 9 ). (aacrjournals.org)
  • Using a well-defined transgenic animal model, they are testing the effectiveness of different peptide sequences identified by phage display to promote antigen uptake and presentation and are using this methodology to study how this might be used to either enhance or suppress helper T cell responses in vivo. (uab.edu)
  • The technology, designed to specifically bind and enter tumor cells and the vasculature supporting tumors, is thought to be the world's largest library of human-derived targeting agents, representing thousands of unique peptide sequences. (nanowerk.com)
  • Figure 1: Panning with a pentavalent peptide library displayed on pIII. (neb.com)
  • The team has previously used an advanced high-throughput screening technique called phage display to discover a small peptide that ultimately was chemically modified to become Vasotide. (unm.edu)
  • With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. (creative-biolabs.com)
  • In this paper we present the in-depth identification and relative quantification of 14,500 peptide ligands constituting the HLA ligandome of B cells. (mcponline.org)
  • In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. (wikipedia.org)
  • Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening the peptide-target interaction, also serves to detach the target molecule from the immobilization surface. (wikipedia.org)
  • This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. (2bscientific.com)
  • A variety of approaches are used to identify targets or determine mechanisms of action, including mutagenesis and genome sequencing, and cell based and functional assays. (nih.gov)
  • While HIV-specific CD4 clones are depleted directly and indirectly by HIV infection, sensitive assays can detect cells capable of producing interferon gamma in response to HIV antigens despite advancing immunodeficiency. (natap.org)
  • Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. (mdpi.com)
  • Although these techniques are very useful as initial screening methods, they do not allow for the identification of the exact molecular target of the autoantibodies. (frontiersin.org)
  • Although excessive cell damage results in passive necrosis, cells can be triggered to engage molecular programs that result in cell death. (bireme.br)
  • Thus, the EGFR is considered a validated molecular biomarker for certain cancers including non-small cell lung, head and neck, colorectal, and ovarian cancers. (aacrjournals.org)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the w:embryo . (wikiversity.org)
  • Using molecular techniques the researchers investigated how the antibody was protecting mice. (www.nhs.uk)
  • It is possible to perform all of these techniques in a standard molecular biology lab or the process can be outsourced to a variety of facilities specializing in the generation of rAbs. (jhsph.edu)
  • This is a fascinating and unconventional route for antigen presentation to T cells, which does not require complex and rate-limiting molecular interactions inside the dendritic cell. (medicalnewstoday.com)
  • To circumvent these problems, researchers including British biochemist Greg Winter invented robust "molecular display" technologies. (harvard.edu)
  • By doing so, Jonkheijm, who heads research into Bioinspired Molecular Engineering, and his team, were able to show that living cells can also be used as a component in supramolecular smart materials. (nanowerk.com)
  • We now demonstrate effects of extracellular potassium on stiffness of individual cultured bovine aortic endothelial cells by using the tip of an atomic force microscope as a mechanical nanosensor. (pnas.org)
  • As potassium has a beneficial effect on cardiovascular function ( , 17 - , 20 ), we have now examined the relation between extracellular potassium and cell stiffness. (pnas.org)
  • AFM imaging of living vascular endothelial cells, exposed to increasing concentrations of extracellular potassium. (pnas.org)
  • Relationship between extracellular potassium concentration and cortical cell stiffness. (pnas.org)
  • Hartline pushed experimental technology of the 1930's to its limits in obtaining extracellular recordings of individual ganglion cells. (utah.edu)
  • Fibronectin containing extracellular material connects surfaces of developing granulocytes with surfaces of stromal cells. (springer.com)
  • Campbell A, Wicha MS, Long M (1985) Extracellular matrix promotes the growth and differentiation of murine hematopoietic cells in vitro. (springer.com)
  • Cells display dozens or even hundreds of molecules at their surface, that receive incoming signals and activate one or more intracellular signaling pathways. (bgsu.edu)
  • They used a technique called 'antibody phage-display library' to produce antibody-like molecules that were highly responsive to this virus. (www.nhs.uk)
  • Using new equipment we developed and a technique called video intensification microscopy, described in a paper in Cell , we began to look at molecules moving about in vesicles within the cell. (nih.gov)
  • Although this study focused specifically on CB[8], the techniques demonstrated by the researchers can also be used to develop and study other strains for different host molecules. (nanowerk.com)
  • Mast cells and NGF appear to be involved in neuroimmune interactions and tissue inflammation, with NGF acting as a general 'alert' molecule capable of recruiting and priming tissue defence processes following insult as well as systemic defensive mechanisms. (embl-heidelberg.de)
  • This finding has led to the development and testing of second-generation small-molecule compounds that could be used clinically to inhibit cell proliferation and target Rad51 interactions. (army.mil)
  • they tend to spread themselves as flat as possible, have minimal cell-cell interactions and display different expression and morphogenesis. (labx.com)
  • It has been selected as a paradigm because the other AB5 toxin sub-families referred to above have been known for many years, and a substantial body of information on toxin-receptor interactions had been built up using conventional biochemical techniques [1] [4] . (functionalglycomics.org)
  • Both techniques neglect individual interactions. (hindawi.com)
  • There is no evidence of fibronectin being associated with blood-stromal cell interactions involving either erythropoiesis or thrombopoiesis. (springer.com)
  • This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface autoantibodies and/or the identification of novel surface antigens. (frontiersin.org)
  • In addition, we discuss the use of immunoprecipitation and mass spectrometry analysis for the detection of novel neuronal surface antigens, which is a crucial step in further disease classification and the development of novel CBAs. (frontiersin.org)
  • For the detection of neuronal surface antigens also immunocytochemistry (ICC) of primary hippocampal neurons is used. (frontiersin.org)
  • These cells play an important role in directing the immune response when foreign antigens are encountered. (natap.org)
  • HIV antigens are presented by DCs to CD4 and CD8 cells, leading to the expansion of HIV-specific CD4 and CD8 clones. (natap.org)
  • They identified populations of T cells responding (i.e. producing interferon gamma) to either HIV antigens or (as a control) CMV antigens, and sorted these cells by flow cytometry. (natap.org)
  • The evidence of humoral immunity to SSX-2 and SSX-4 , and SSX-4 -specific CD4 + T cells among circulating lymphocytes in patients with antigen expressing EOC suggest that these antigens are attractive targets for specific immunotherapy in EOC. (aacrjournals.org)
  • The development of immunotherapeutic strategies in EOC requires the identification of tumor antigens expressed in these tumors and the design of vaccination strategies that may result in the generation of tumor antigen-specific T cells that could afford durable protection. (aacrjournals.org)
  • The few in vitro studies investigating the impact of malaria on HIV-1 have demonstrated that exposure to soluble malarial antigens can increase HIV-1 infection and reactivation in immune cells. (jove.com)
  • They capture antigens from foreign bodies and hand them over to killer T cells, which then neutralize the threat. (medicalnewstoday.com)
  • To create dendritic cell vaccines, dendritic cells are removed from the patient and "force-fed" tumor antigens before being released back into the patient. (medicalnewstoday.com)
  • One major drawback is that the tumor antigens used to "feed" the dendritic cells are from lab-grown tumors, not the patient's own. (medicalnewstoday.com)
  • Two images of EVIR-engineered dendritic cells (green) capturing tumor antigens in exosomes (gold/red). (medicalnewstoday.com)
  • Using imaging techniques, the team demonstrated that EVIRs enhanced transfer of tumor antigens from the exosome to the outer membrane of the dendritic cell. (medicalnewstoday.com)
  • We call this phenomenon cross-dressing, which alludes to the fact that the dendritic cells acquire immunogenic antigens from the tumor and directly display them on their own surface. (medicalnewstoday.com)
  • Investigations by fast reaction techniques with membrane vesicles, Biochemistry 20: 3467 (1981). (springer.com)
  • Endothelial cells are softened by estrogens through activation of a plasma membrane sodium/proton antiporter, yet are insensitive to progesterone and testosterone ( , 11 ). (pnas.org)
  • In agreement with a previous report ( , 21 ), we assume that the first slope represents the plasma membrane stiffness and includes a contribution from the submembrane cortical cytoskeleton, whereas the second slope, which is approximately three times larger than the first slope, represents the stiffness of the inner bulk of the cell. (pnas.org)
  • Certain cell signaling pathways are implemented by assembling their components on scaffolds and/or restricting their lateral mobility in a membrane. (bgsu.edu)
  • The biophysical model suggested that cicada wing nanopillars induce physical stretching of the cell membrane upon contact, leading to bacterial rupture and death, and cell rigidity was reported to be an important determinant of susceptibility 14 . (nature.com)
  • Each bacterium generates several enzymes and displays them on its outer membrane. (igem.org)
  • Could this be done by displaying them on a cell outer membrane? (igem.org)
  • that were all located in surface-exposed loops of the trimeric outer membrane porin, aiming at developing multivalent live bacterial vaccines (12-15). (wikipedia.org)
  • Podosomes are conical, actin-rich structures found on the outer surface of the plasma membrane of animal cells. (wikipedia.org)
  • While usually situated on the periphery of the cellular membrane, these unique structures display a polarized pattern of distribution in migrating cells, situating at the front border between the lamellipodium and lamellum. (wikipedia.org)
  • The method harnesses the intrinsic intracellular folding quality control mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway to display an scFv library on the E. coli inner membrane. (jove.com)
  • Following removal of the outer membrane, the scFvs displayed on the inner membrane are panned against a target antigen immobilized on magnetic beads to isolate scFvs that bind to the target antigen. (jove.com)
  • It has been previously reported that human leukocyte elastase (HLE) is involved in plasma membrane events during stimulation of immune cells, (Bristow and Flood, T Cell Antigen Immune Complexes Demonstrating Biologic & Proteolytic Activity , Int. Immunol. (freepatentsonline.com)
  • However, cell-surface lipids are known to negatively influence catalytic activity (Bangalore and Travis, Comparison Of Properties OF Membrane Bound Versus or modulate such response to various disease states by control of HLE involvement in such processes. (freepatentsonline.com)
  • SefA was verified located on the cell surface, and H:gm was found in the outer membrane of the host cell, though only in proteolytically truncated forms lacking the His 6 -tag used for detection. (diva-portal.org)
  • For example, TonB-dependent transporters (TBDTs) in the outer membrane of Gram-negative bacteria play an essential role transporting iron and other nutrients into the bacterial cell. (rsc.org)
  • Individual candidates were evaluated for relative binding to fluorescently-labeled PA target or streptavidin negative control using Fluorescence-Activated Cell Sorting (FACS). (omicsonline.org)
  • Bacterial display is often coupled with magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS) techniques. (wikipedia.org)
  • rAbs can be used in all applications in which traditional mAbs are used such as western blotting, immunohistochemistry, fluorescence-activated cell sorting (FACS), and immunofluorescence. (prolabmarketing.com)
  • Granit also made the first ganglion cell recordings from a mammalian retina, the cat retina. (utah.edu)
  • TR is expressed in nearly all mammalian cells and its itinerary is well characterized by us and others. (uab.edu)
  • During the first plenary lecture on Monday (L2), Melissa Pope discussed the role of dendritic cells (DCs) in HIV infection and pathogenesis. (natap.org)
  • Many different specialized cells exhibit these dynamic structures such as invasive cancer cells, osteoclasts, vascular smooth muscle cells, endothelial cells, and certain immune cells like macrophages and dendritic cells. (wikipedia.org)
  • Examples of these motile cell behaviors include: transendothelial migration of dendritic cells, migration of aortic endothelial cells for arterial vessel remodeling, and tissue infiltration by macrophages. (wikipedia.org)
  • The dendritic cells and macrophages of these patients' immune systems do not manifest podosome formations and demonstrate defects in cellular movement within tissue microenvironments. (wikipedia.org)
  • Recent work has demonstrated that exposure of immature monocyte-derived dendritic cells to the malarial pigment hemozoin increased their ability to transfer HIV-1 to CD4+ T cells 6,7 , but that it decreased HIV-1 infection of macrophages 8 . (jove.com)
  • Specifically, the Swiss group is interested in improving so-called dendritic cell vaccines . (medicalnewstoday.com)
  • Dendritic cells - also referred to as antigen-presenting cells - are an important part of the immune system. (medicalnewstoday.com)
  • Dendritic cell vaccines have already shown promise, but they do have limitations. (medicalnewstoday.com)
  • Dendritic cells are extracted from a patient and EVIRs are inserted into them. (medicalnewstoday.com)
  • When the dendritic cells are returned to the patient's body, they are primed to recognize types of small vesicle called exosomes . (medicalnewstoday.com)
  • EVIRs trap exosomes traveling through the body, giving dendritic cells the exact blueprint of the resident tumor. (medicalnewstoday.com)
  • The dendritic cells can then inform the killer T cells and boost the patient's immune response to the cancer. (medicalnewstoday.com)
  • Tissue sections were analyzed for cell density, cell organization, ECM components, ECM-degrading enzymes and markers of differentiation, proliferation, and stem cells. (biomedsearch.com)
  • In contrast, ACL degeneration is associated with cell recruitment or proliferation, including progenitor cells or myofibroblasts. (biomedsearch.com)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves, and then differentiate into the cell types needing replacement in the damaged tissue [26] Macrophages can self-renew by local proliferation of mature differentiated cells. (wikiversity.org)
  • 1 In the second compartment, terminal maturation is accompanied by a decrease in proliferation, an increase in cell ploidy, and cytoplasmic maturation. (bloodjournal.org)
  • Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. (asm.org)
  • Initial attachment of cells is followed by proliferation and biofilm formation, with sessile cells in biofilms displaying properties that are dramatically different from their planktonic (free-living) counterparts. (asm.org)
  • I will highlight a strategy that uses phage display as a starting point that utilizes quantitative and in silico methodologies as a means of rapidly generating high affinity targeted ligands for use in therapy and imaging. (pegsummit.com)
  • This technique streamlines isolation of stable MP variants for rapid characterization of binding to various ligands. (rsc.org)
  • Surfaces displaying nano- and micropatterned cell adhesive ligands have led to numerous discoveries in cell biology. (asme.org)
  • These HLA ligands can form the basis for reversed immunology approaches to identify T cell epitopes based not on in silico predictions but on the bona fide eluted HLA ligandome. (mcponline.org)
  • The most common bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also been used. (wikipedia.org)
  • This antibody, raised against native p67, also specifically recognized the surface of recombinant-infected cells. (wikigenes.org)
  • A collection of recombinant phage that display unique antigen binding domains on their surfaces is known as a phage display library. (jhsph.edu)
  • Sera from a subgroup of the patients were tested for SSX-2 and SSX-4 antibody by ELISA and recombinant antigen expression on yeast surface (RAYS). (aacrjournals.org)
  • E. coli displaying multiple enzymes. (igem.org)
  • Additionally, we want to make one of these aptamers bind to the E. coli cell surface. (openwetware.org)
  • What aptamers exist that bind the E. coli cell surface? (openwetware.org)
  • Then, the cDNA clones encoding variable heavy and variable light chains of these four mabs were isolated, and used for construction of four single-chain Fv (scFv) antibody genes, which were expressed in Escherichia coli cells. (go.jp)
  • The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into E. coli bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
  • SubB has recently been shown to bind to N-linked glycans displayed on several glycoproteins on the surface of Vero and HeLa cells, including α2β1integrin [5] . (functionalglycomics.org)
  • Each ShuA variant was analyzed for its ability to display on the bacteriophage surface, and functionally bind to hemoglobin. (rsc.org)
  • PLA MCs suitable for Mesenchymal Stromal Cells (MSCs) homing have been produced and modified with different functionalization approaches. (upc.edu)
  • The central hypothesis of the STELLAR project is that kidney stromal cells (kPSCs) confer specific regenerative and homeostatic functions with respect to kidney regeneration as compared to mesenchymal stromal cells (MSC) isolated from other tissues such as bone marrow or umbilical cord. (europa.eu)
  • The STELLAR consortium proposes to develop an alternative to renal replacement therapy, based on the repair capacity of newly discovered kidney mesenchymal stromal cells (kPSCs). (europa.eu)
  • Much of the fibronectin appears to be situated on surfaces of those fibroblastic stromal cells which support granulopoiesis. (springer.com)
  • Fibronectin is a surface component of granulocytes as well as nearby stromal cells. (springer.com)
  • These studies indicate that fibronectin is situated at sites where it could mediate adhesive interaction between granulopoietic cells and their stromal cells. (springer.com)
  • This is strengthening tissue directly below the surface of the roots, characterised by particularly small cells with thick walls. (rural21.com)
  • Moreover, mast cells themselves produce NGF, suggesting that alterations in normal mast cell behaviours can provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states. (embl-heidelberg.de)
  • The physic-chemical properties of the particles can be engineered to obtain smart scaffolds for tissue engineering, carriers for drug release and cell therapy. (upc.edu)
  • Taken together, the injectable devices developed in the Thesis constitute promising and highly versatile biomaterial platforms for biomedical applications, and can be employed in a wide array of tissue engineering, and cell and drug delivery strategies. (upc.edu)
  • [27] In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikiversity.org)
  • Stem cells have demonstrated remarkable potential for tissue engineering and regenerative medicine through their ability for self-renewal and controlled differentiation. (nanohub.org)
  • Consequently, podosomes are present in cell types associated with tissue remodeling and the immune system. (wikipedia.org)
  • Bentley SA, Foidart J-M (1980) Some properties of marrow derived adherent cells in tissue culture. (springer.com)
  • The technique could be used to make novel optical devices or biological scaffolds to grow soft tissue, teeth, and bone. (technologyreview.com)
  • We have addressed this issue for two intimately related serine proteases, tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (uPA), by modifying the technique of substrate phage display to create substrate subtraction libraries. (elsevier.com)
  • Profilee Satyajit Mayor discusses his explorations of cell membranes, which are helping to update the classical fluid mosaic model of dynamic cellular boundaries. (the-scientist.com)
  • While some advances have been made by other groups where supramolecular elements were introduced on cell membranes by merging artificially made membranes, this is the first time where the cells were made to produce and display supramolecular elements on their surfaces through genetic engineering. (nanowerk.com)
  • In the presence of aldosterone, plasma sodium in the high physiological range stiffens endothelial cells and reduces the release of nitric oxide. (pnas.org)
  • An acute increase of potassium in the physiological range swells and softens the endothelial cell and increases the release of nitric oxide. (pnas.org)
  • Superficial and Deep Stiffness Are Detectable in Living Endothelial Cells. (pnas.org)
  • Typical indentation curves of living endothelial cells are not exponential in shape. (pnas.org)
  • Indentation curves obtained in 2 experiments on vascular endothelial cells. (pnas.org)
  • Nitrite concentrations analyzed in the supernatant culture media after exposure of the vascular endothelial cells for 24 h to different potassium concentrations. (pnas.org)
  • OxTrp72-apoA1 recovered from human atheroma or plasma is lipid poor, virtually devoid of cholesterol acceptor activity, and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo . (acc.org)
  • Endothelial cells line the inside of blood vessels of normal organs and tumors. (unm.edu)
  • It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. (nih.gov)
  • In this way, killer T cells are primed to destroy tumor cells, which are normally experts at evading the immune system. (medicalnewstoday.com)
  • Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning). (wikipedia.org)
  • Flow cytometry is a unique experimental technology that provides rapid, quantitative, multiparametric, single cell analysis and separation. (cancer.gov)
  • Instrumentation includes two customized three-laser (two tunable argon or krypton and rhodamine 6G argon-pumped dye laser) flow cytometers with electronic cell separation capabilities that are operated by staff of the Flow Cytometry Laboratory in support of multiple research projects. (cancer.gov)
  • In addition, staff provide training and consultation in flow cytometry techniques, protocol design, reagent selection, and data analysis and presentation. (cancer.gov)
  • Customized flow cytometry data analysis and data archiving software is developed and maintained, including automated cluster analysis software for statistical classification of quantitative multiparametric data and n-plot graphics display of multiparameter data on either subpopulations or individual cells. (cancer.gov)
  • Since Saccharomyces cerevisiae lacks the cellulase complexes that hydrolyze cellulosic materials, which are abundant in the world, two types of hydrolytic enzymes involved in the degradation of cellulosic materials to glucose were genetically co-immobilized on its cell surface for direct utilization of cellulosic materials, one of the final goals of our studies. (asm.org)
  • This result showed that the cell surface-engineered yeast with these enzymes can be endowed with the ability to assimilate cellooligosaccharides. (asm.org)
  • Since enzymatic hydrolysis of cellulose has the potential to surmount many of the drawbacks of acid hydrolysis ( 12 , 13 ), we have attempted to genetically immobilize cellulolytic enzymes in their active form on the cell surface of the yeast S. cerevisiae to construct a novel cellulose-utilizing yeast. (asm.org)
  • This anchoring signal was combined with the signal of the secreted enzymes by genetic engineering techniques. (asm.org)
  • Here, we show that biosynthetic enzymes can display unexpected promiscuity, with human glycosyltransferase pp-a-GanT2 able to utilize both uridine diphosphate N-acetylglucosamine and uridine diphosphate N-acetylgalactosamine, leading to the synthesis of epimeric glycopeptides in vitro. (jic.ac.uk)
  • M13 phage displaying multiple enzymes. (igem.org)
  • Furthermore, hemicellulolytic enzymes displayed on the yeast cell surface hydrolyzed hemicelluloses that were not hydrolyzed by a commercial enzyme, leading to increased sugar utilization for improved ethanol production. (biomedcentral.com)
  • The surface expression of enzymes and vaccine epitopes offer several advantages. (diva-portal.org)
  • Surface expressed enzymes gain similar properties to immobilised enzymes, mainly simplified handling and separation using centrifugation. (diva-portal.org)
  • I was mainly interested in how the enzymes within the cell work. (nih.gov)
  • These results demonstrate that the combination of substrate phage display and substrate subtraction methods can be used to discover specificity differences between very closely related enzymes and that this information can be utilized to create highly selective inhibitors. (elsevier.com)
  • Two new approaches allow kinetic measurements of receptor function to be made on cell surfaces with a time resolution of μs to ms. (springer.com)
  • Hess, G.P. and Udgaonkar, J.B., Chemical kinetic measurements of transmembrane processes using rapid reaction techniques: Acetylcholine receptor, Ann. (springer.com)
  • Shusta EV, Holler PD, Kieke MC et al (2000) Directed evolution of a stable scaffold for T-cell receptor engineering. (springer.com)
  • 1. An isolated stem cell, which is isolated by a method, comprising selecting the stem cell based on the stem cell exhibiting a CXCR4 receptor, demonstrating an affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Integral in the regulation and initiation of death receptor-mediated activation of programmed cell death is the aspartate-specific cysteine protease (caspase)-8. (bireme.br)
  • We have developed a near-infrared (NIR) probe that targets cells overexpressing the EGF receptor (EGFR) for imaging glioblastoma brain tumors in live subjects. (aacrjournals.org)
  • It binds as a dimer to at least 2 cell-surface receptor types expressed in a variety of neuronal and non-neuronal cells. (embl-heidelberg.de)
  • Early genes, such as mpl coding for the TPO receptor 9 and the GPIIb gene 10 are first expressed in CD34 + cells, but are still expressed in the late stages leading to platelet production. (bloodjournal.org)
  • TNF reduced production of infectious viral particles by Huh-7 and HFLC, and thereby reduced the number of infected cells and focus size. (bireme.br)
  • While MDCs are inefficiently infected, the presentation of HIV particles by MDCs to T cells provides an unfortunately optimal means of driving T cell infection and high-grade HIV production. (natap.org)
  • On the other hand, larger particles such as MC, display a high surface area for cell expansion. (upc.edu)
  • However, there appear to be fewer ferritin particles per unit of surface on granulocytic cells. (springer.com)
  • Many of the ferritin particles are not clearly associated with amorphous matrix material at cell surfaces. (springer.com)
  • Through an in vivo approach, the genotype-phenotype link is produced by using a living cell. (hindawi.com)
  • These studies employ both cell biological and immunological techniques to understand how helper T cell responses are regulated in vitro and in vivo. (uab.edu)
  • Furthermore, to improve upon current in vivo gene delivery methods, COE investigators have engineered specialized expression vectors with cancer-specific promoters, a two-step transcriptional activation system, and post-translational regulatory elements that are capable of expressing therapeutic genes by several hundred-fold in breast cancer cells only. (army.mil)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into w:endodermal , ectodermal and mesodermal cells both in vitro and in vivo. (wikiversity.org)
  • In addition to multiple implications in the regenerative potential of other tissues, mesenchymal stem cells have been shown to promote vessel formation both in vivo and in vitro. (nanohub.org)
  • 3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (labx.com)
  • Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (labx.com)
  • Consequently, administration of anti-CD25 Abs is a commonly used technique to deplete Treg populations in vivo. (nih.gov)
  • Bacteriophage display is the most common type of display system used although bacterial display is becoming increasingly popular as technical challenges are overcome. (wikipedia.org)
  • Bacterial display combined with FACS also has the advantage that it is a real-time technique. (wikipedia.org)
  • NHEs have various physiological functions including intracellular pH homeostasis, cell volume regulation, acid-base regulation, and electroneutral NaCl transport ( 22 , 53 ). (physiology.org)
  • and 6) separation and analyses of genetically engineered cells for studies of intracellular processes. (cancer.gov)
  • Bacterial display sorting is a powerful emerging technology that offers a rapid, high-throughput approach to discovery of robust affinity reagents [ 1 - 3 ]. (omicsonline.org)
  • In addition, the technique can be scaled up for the high-throughput approaches of genomics and proteomics. (harvard.edu)
  • However, these studies used whole cell extracts of P. falciparum schizont stage parasites and peripheral blood mononuclear cells (PBMC), making it hard to decipher which malarial component(s) was responsible for the observed effects and what the target host cells were 4,5 . (jove.com)
  • Using phage display techniques, a high-affinity monoclonal antibody was developed that specifically recognizes both apolipoprotein A1 (apoA1) and HDL that have been modified by the MPO-H 2 O 2 -Cl− system. (acc.org)
  • A simple design of a yeast-displayed heterodimeric Fc fragment is described and can be used as a general guideline for affinity selection of Fcabs with an asymmetric binding site. (springer.com)
  • and assessing tumor tropic potential of the stem cell based upon said expression level of CXCR4, said affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity reagents and direct amplification of bound cells without the need to elute and reinfect. (omicsonline.org)
  • Actually, we reported previously the genetic immobilization of carboxymethylcellulase (CMCase) from Aspergillus aculeatus on the yeast cell surface ( 16 ). (asm.org)
  • Each variable region gene is spliced into a vector (vehicles that transfer foreign genetic material into another cell), and the vector is taken up and integrated into the genome of a microorganism. (jhsph.edu)
  • To address these issues, he and his laboratory members take a combined biochemical, cell biological, and genetic approach. (cancer.gov)
  • Here we report the engineering and characterization of phage display libraries of stable human VH domains. (pegsummit.com)
  • Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided sufficient material to enable its biochemical characterization. (embl-heidelberg.de)
  • Akiyama SK, Yamada SS, Yamada KM (1986) Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule. (springer.com)
  • Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, McKenzie S, Broxmeyer HE, Moore MAS (1980) Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (springer.com)
  • After mixing them with the non-genetically modified lactic acid bacteria cells, the fused GFP-LcsB and Gal-LcsB were functionally associated with the cell surface of various lactic acid bacteria tested. (biomedcentral.com)
  • Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. (unt.edu)
  • Using a single virus as a building unit is "pretty exquisite," says Belcher, because its traits can be genetically modified and you can attach many different useful materials to its surface. (technologyreview.com)
  • This makes it difficult to study the nodes where pathways interact using traditional biochemical techniques. (bgsu.edu)
  • Surface expressed vaccine epitopes can have longer half-lives inside the animal that is to be immunized and surface groups on the host cell can act as adjuvants, increasing the immune response and leading to a better immunisation. (diva-portal.org)
  • Traxlmayr MW, Faissner M, Stadlmayr G et al (2012) Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast. (springer.com)
  • However, the development of optimal three dimensional (3D) scaffolds that overcome the limitations of 2D cell culture remains. (nanohub.org)
  • OMPs are common scaffolds for bacterial display. (wikipedia.org)
  • Conceivably both techniques might be utilized by different components of the same research group, and active products of the biosynthetic approach may serve as novel scaffolds for elaboration using combinatorial synthetic technology. (nih.gov)
  • This Review will focus on a subset of synthetic biology tools known as bottom-up techniques, which use technologies such as optogenetics and chemically induced dimerization to reconstitute cellular behavior in cells. (sciencemag.org)
  • International Journal of Cell Biology , Article ID 402916, 16 pages. (bgsu.edu)
  • For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (labx.com)
  • Patients who suffer from Wiskott-Aldrich syndrome demonstrate, through their immune cells, continued evidence of the role podosomes fulfill in cell motility. (wikipedia.org)
  • Ali IU, Hynes RO (1978) Effects of LETS glycoprotein on cell motility. (springer.com)
  • Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. (springer.com)
  • Traxlmayr MW, Lobner E, Antes B et al (2013) Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display. (springer.com)
  • Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. (illinois.edu)
  • Another exciting application of injectable, cell-laden MCs is to use them as building blocks to fabricate living tissues in vitro. (upc.edu)
  • a ) predominant expression in germ cells of the testis and generally not in other normal tissues, ( b ) expression in a proportion of malignant tumors of different histologic types, ( c ) expression in malignancies in a lineage nonspecific fashion, ( d ) often mapping of the gene on the X chromosome, ( e ) often members of multigene families. (aacrjournals.org)
  • Jonkheijm's group has also been actively developing such dynamic and responsive materials to address biomolecules, cells and even tissues. (nanowerk.com)
  • These investigations involve quantitative, single cell analyses of parameters associated with cells freshly prepared from different species/tissues as well as a wide spectrum of in vitro cultured cells. (cancer.gov)
  • TNF inhibits HCV infection despite increased HCV envelope glycoprotein-mediated infection of liver cells. (bireme.br)
  • Asthma is a chronic inflammatory disease of the airways in which innate and adaptive immune cells participate as drivers of the inflammatory response. (uab.edu)
  • Biological phenomena, such as cellular differentiation and phagocytosis, are fundamental processes that enable cells to fulfill important physiological roles in multicellular organisms. (sciencemag.org)
  • Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells . (wikiversity.org)
  • This meant that the cells can change their differentiation pathway. (wikiversity.org)
  • In w:Drosophila imaginal discs, cells have to choose from a limited number of standard discrete differentiation states. (wikiversity.org)
  • The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikiversity.org)
  • Human cord blood CD34 + progenitor cells were cultured in the presence of cytokines inducing megakaryocytic differentiation. (bloodjournal.org)
  • We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). (nih.gov)
  • Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. (frontiersin.org)
  • Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. (mdpi.com)
  • However, widespread use is limited, in part due to poor performance achieved using manual Magnetic-Activated Cell Sorting (MACS) methods, and an absence of widely-available, low cost, high-performance sorting alternatives. (omicsonline.org)
  • Professor Ki Hean Kim explained about his future plans, "This study is meaningful, because the new imaging method overcame the limitations of conventional examination methods of conjunctival goblet cells. (postech.ac.kr)
  • In contrast to manual MACS, the bacterial display library was preferentially enriched by autoMACS® sorting, yielding several promising candidates after only three rounds of biopanning and bioinformatic analysis. (omicsonline.org)
  • Bacterial display systems were first introduced by Freudl et al. (wikipedia.org)
  • 6. The isolated stem cell of claim 5, wherein said heterologous gene encodes a polypeptide of therapeutic use in the treatment of a disease condition. (freepatentsonline.com)