Cell surface display techniques. Medical search

Cell Surface Display Techniques: Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Peptide Library: A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Thiolester Hydrolases Neurospora crassa: A species of ascomycetous fungi of the family Sordariaceae, order SORDARIALES, much used in biochemical, genetic, and physiologic studies.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Alberta: A province of western Canada, lying between the provinces of British Columbia and Saskatchewan. Its capital is Edmonton. It was named in honor of Princess Louise Caroline Alberta, the fourth daughter of Queen Victoria. (From Webster's New Geographical Dictionary, 1988, p26 & Room, Brewer's Dictionary of Names, 1992, p12)Neurospora: A genus of ascomycetous fungi, family Sordariaceae, order SORDARIALES, comprising bread molds. They are capable of converting tryptophan to nicotinic acid and are used extensively in genetic and enzyme research. (Dorland, 27th ed)Waste Products: Debris resulting from a process that is of no further use to the system producing it. The concept includes materials discharged from or stored in a system in inert form as a by-product of vital activities. (From Webster's New Collegiate Dictionary, 1981)SELEX Aptamer Technique: A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS.Aptamers, Nucleotide: Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.Aptamers, Peptide: Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Protein Engineering: Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.Animal Husbandry: The science of breeding, feeding and care of domestic animals; includes housing and nutrition.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Livestock: Domesticated farm animals raised for home use or profit but excluding POULTRY. Typically livestock includes CATTLE; SHEEP; HORSES; SWINE; GOATS; and others.Animals, Domestic: Animals which have become adapted through breeding in captivity to a life intimately associated with humans. They include animals domesticated by humans to live and breed in a tame condition on farms or ranches for economic reasons, including LIVESTOCK (specifically CATTLE; SHEEP; HORSES; etc.), POULTRY; and those raised or kept for pleasure and companionship, e.g., PETS; or specifically DOGS; CATS; etc.Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.ADAM Proteins: A family of membrane-anchored glycoproteins that contain a disintegrin and metalloprotease domain. They are responsible for the proteolytic cleavage of many transmembrane proteins and the release of their extracellular domain.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Single-Chain Antibodies: A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.Immunoglobulin Fragments: Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.Disintegrins: A family of polypeptides purified from snake venoms, which contain the arginine-glycine-aspartic acid (RGD) sequence. The RGD tripeptide binds to integrin receptors and thus competitively inhibits normal integrin-ligand interactions. Disintegrins thus block adhesive functions and act as platelet aggregation inhibitors.Immediate Dental Implant Loading: Endosseous dental implantation where implants are fitted with an abutment or where an implant with a transmucosal coronal portion is used immediately (within 1 week) after the initial extraction. Conventionally, the implantation is performed in two stages with more than two months in between the stages.Directed Molecular Evolution: The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Frameshift Mutation: A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Bacteriophage M13: Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.Bacteriophages: Viruses whose hosts are bacterial cells.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Immunoglobulin Fab Fragments: Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.Firefighters: Professional or volunteer members of a fire department who are trained to suppress fire and respond to related emergency.HIV Antigens: Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.HIV Infections: Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).Allergy and Immunology: A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.HIV-1: The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.Cytomegalovirus: A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.Cytomegalovirus Infections: Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.Conjunctiva: The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.Tears: The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.Epithelium, Corneal: Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Sodium-Hydrogen Antiporter: A plasma membrane exchange glycoprotein transporter that functions in intracellular pH regulation, cell volume regulation, and cellular response to many different hormones and mitogens.Mucin-4: A transmembrane mucin that is found in a broad variety of epithelial tissue. Mucin-4 may play a role in regulating cellular adhesion and in cell surface signaling from the ERBB-2 RECEPTOR PROTEIN-TYROSINE KINASE. Mucin-4 is a heterodimer of alpha and beta chains. The alpha and beta chains result from the proteolytic cleavage of a precursor protein.Lacrimal Apparatus: The tear-forming and tear-conducting system which includes the lacrimal glands, eyelid margins, conjunctival sac, and the tear drainage system.

Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes. (1/81)

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Relevant uses of surface proteins--display on self-organized biological structures. (2/81)

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SplitCore: an exceptionally versatile viral nanoparticle for native whole protein display regardless of 3D structure. (3/81)

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Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display. (4/81)

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Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin. (5/81)

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Bacillus subtilis: a temperature resistant and needle free delivery system of immunogens. (6/81)

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Baculovirus as a vaccine vector. (7/81)

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Surface display of N-terminally anchored invasin by Lactobacillus plantarum activates NF-kappaB in monocytes. (8/81)

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Glypican 4

2001). "Cell surface glypicans are low-affinity endostatin receptors". Mol. Cell. 7 (4): 811-22. doi:10.1016/S1097-2765(01) ... 2002). "Use of a phage display technique to identify potential osteoblast binding sites within osteoclast lacunae". J. Bone ... Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable ... These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end ...

Podosome

Morton, PE; Parsons, M (Jul-Aug 2011). "Dissecting cell adhesion architecture using advanced imaging techniques". Cell adhesion ... Podosomes are conical, actin-rich structures found on the outer surface of the plasma membrane of animal cells. Their size ... but new advances in bioimaging techniques have altered that perception and show the ring complex to display a polygonal form. ... vascular smooth muscle cells, endothelial cells, and certain immune cells like macrophages and dendritic cells. A podosome ...

Nutritional genomics

... for the regulated reverse transport of animal and plant dietary sterols out of the apical surface of intestinal cells. By ... Differential display mode was used by comparing differences in expression levels with a control group of mice. This led to the ... Through computer simulation techniques, it was found that two proteins produced by the newly discovered gene were responsible ... Usually, these technologies are applied in a "differential display" mode, i.e. by comparing two situations (e.g. diseased ...

Luciferase

... is a powerful technique for studying cell populations in live animals, such as mice. Different types of cells (e.g. bone marrow ... This allows luciferase to detect the efflux of ATP from the cell and will effectively display the real-time release of ATP ... Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E (May 2006). "Cell-surface-localized ATP detection with ... Biotinylation will immobilize luciferase on the cell-surface by binding to a streptavidin-biotin complex. ...

Solar cell

Surface texturing is one of techniques used to reduce optical losses to maximize light absorbed. Currently, surface texturing ... Solar panels using mono-Si cells display a distinctive pattern of small white diamonds. ... Perovskite solar cells[edit]. Main article: Perovskite solar cell. Perovskite solar cells are solar cells that include a ... Main articles: Organic solar cell and Polymer solar cell. Organic solar cells and polymer solar cells are built from thin films ...

Bacterial display

This was the first evidence of using bacterial surface display techniques to express proteins on the surface of cells, without ... Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro ... Bacterial display can be used to find peptides which bind to specific cells e.g. breast cancer cells or stem cells. Displayed ... Bacterial display combined with FACS also has the advantage that it is a real-time technique. Cell display systems were first ...

Hidden surface determination

... while a C-Buffer requires polygons to be displayed from the nearest to the furthest. Because the C-buffer technique does not ... Portal rendering divides a scene into cells/sectors (rooms) and portals (doors), and computes which sectors are visible by ... or visible surface determination (VSD)) is the process used to determine which surfaces and parts of surfaces are not visible ... There are many techniques for hidden surface determination. They are fundamentally an exercise in sorting, and usually vary in ...

Yeast display

... (or yeast surface display) is a protein engineering technique that uses the expression of recombinant proteins ... The Aga2p protein is naturally used by yeast to mediate cell-cell contacts during yeast cell mating. As such, display of a ... ribosome display, bacterial display, and mRNA display. Gai, S Annie; Wittrup, K Dane (2007). "Yeast surface display for protein ... The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. The technology was sold to ...

B-1 cell

B1 B cells characteristically express high levels of surface IgM (sIgM), demonstrable CD11b, and low levels of surface IgD ( ... B1 B cells self-renew and spontaneously secrete IgM and IgG3 serum antibodies. These natural serum antibodies display extensive ... from a mouse by injecting cell medium or PBS into the peritoneal cavity of the mouse and then draining it off via a technique ... Notably, most B1 cells do not develop into memory B cells. B1b cells have been shown to be capable of memory responses. See " ...

Ectomycorrhiza

... they press against the cell walls of the phytobiont's root cells. Often the fungal and plant cell walls become almost ... With the advent of more precise genetic techniques, these traits of the mantle are often used in tandem with molecular analyses ... Much as the Hartig net and mantle, composite hyphal organs can display a wide range of structural difference. Some rhizomorphs ... However, this is largely absent in the cortical type, suggesting different strategies of increasing surface contact among ...

Microbead (research)

Microbeads display a large surface area per volume. This together with uniformity of size and shape provides for very good ... Microspheres Kemshead, JT, Ugelstad, J (1985). "Magnetic separation techniques:their application to medicine", Mol Cell Biochem ... Bio-reactive molecules can be absorbed or coupled to their surface, and used to separate biological materials such as cells, ... Microbeads are used for cell isolation and cell expansion. Proteins and protein complexes can be separated, e.g. in ...

Screen printing

... is more versatile than traditional printing techniques. The surface does not have to be printed under pressure ... This makes it useful for some of the techniques of printing solar cells, electronics etc. ... Graphic screenprinting is widely used today to create mass or large batch produced graphics, such as posters or display stands ... Subsequently, aluminum paste is dispensed over the whole surface of the back for passivation and surface reflection. One of the ...

Atomic force microscopy

The surface topography is commonly displayed as a pseudocolor plot. In manipulation, the forces between tip and sample can also ... In cellular biology, AFM can be used to attempt to distinguish cancer cells and normal cells based on a hardness of cells, and ... In fact, the majority of SPM techniques are extensions of AFM that use this modality. The major difference between atomic force ... Close to the surface of the sample, attractive forces can be quite strong, causing the tip to "snap-in" to the surface. Thus, ...

Immunohistochemistry

Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ... While this technique utilizes only one antibody and therefore is simple and rapid, the sensitivity is lower due to little ... The Human Protein Atlas displays a map of protein expression in normal human organs and tissues and organs. The combination of ... Identification of B-cell lymphomas using CD20 Identification of T-cell lymphomas using CD3 A variety of molecular pathways are ...

Vaa3D

These 3D surface objects are also often arranged as "sets". Vaa3D can display multiple sets of any of these surface objects, ... The Virtual Finger technique maps the identified 2D user input via 2D display devices, such as a computer screen, back to the ... and fast cell counting in specific brain areas. Vaa3D also provides methods to annotate these cells and identify their names. ... Irregular surface objects: Each 3D surface has irregular shape and is modeled using complicated surface mesh. ...

Lipid bilayer

When they compared the area of the monolayer to the surface area of the cells, they found a ratio of two to one. Later analyses ... During programmed cell death a protein called a scramblase equilibrates this distribution, displaying phosphatidylserine on the ... Experimentally, electroporation is used to introduce hydrophilic molecules into cells. It is a particularly useful technique ... 5.1 Cell Membrane Structure , Life Science , University of Tokyo Alberts, Bruce (2002). Molecular biology of the cell (4th ed ...

Immunohistochemistry

Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ... Display techniques. *Bacterial display. *mRNA display. *Phage display. *Ribosome display. *Yeast display ... CD117 (KIT): for gastrointestinal stromal tumors (GIST) and mast cell tumors. *CD10 (CALLA): for renal cell carcinoma and acute ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...

Axon guidance

Ephrins: Ephrins are cell surface molecules that activate Eph receptors on the surface of other cells. This interaction can be ... In addition, severed commissural axons in chicks, display the capability of translating and expressing Eph-A2 receptor during ... Contemporary diffusion-weighted MRI techniques may also uncover the macroscopical process of axonal development. The connectome ... and activate complexes of cell-surface receptors called Plexins and Neuropilins. Cell adhesion molecules (CAMs): Integral ...

Screen printing

Human genetic resistance to malaria

... on the infected red blood cell surface and the modified display of malaria surface proteins reduces parasite adhesiveness ( ... The molecular basis of sickle cell anemia was finally elucidated in 1959, when Ingram perfected the techniques of tryptic ... In addition, binding of parasitized sickle cells to endothelial cells is significantly decreased because of an altered display ... G6PD is present in all human cells but is particularly important to red blood cells. Since mature red blood cells lack nuclei ...

Pixel

In some displays, such as LCD, LED, and plasma displays, these single-color regions are separately addressable elements, which ... Some authors explain pixel as picture cell, as early as 1972. In graphics and in image and video processing, pel is often used ... Pixilation, spelled with a second i, is an unrelated filmmaking technique that dates to the beginnings of cinema, in which live ... Foley, James D.; Andries van Dam; John F. Hughes; Steven K. Feiner (1990). "Spatial-partitioning representations; Surface ...

Phage display

... so that they are displayed on the surface of the viral particle. The protein displayed corresponds to the genetic sequence ... Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. If a "phagemid" vector is used (a simplified display construct ... Competing methods for in vitro protein evolution include yeast display, bacterial display, ribosome display, and mRNA display. ...

Protein engineering

These selected phages are then subjected to DNA sequencing.[page needed] Cell surface display systems can also be utilized to ... Generally these techniques require high sequence homology between parental sequences. These techniques are often used to ... Display: Bacterial display Phage display mRNA display Ribosome display Yeast display Biomolecular engineering Enzymology ... These methods include mRNA display, ribosome display, covalent and non covalent DNA display, and in vitro compartmentalization ...

Gas vesicle

In vitro assessments of cells show expression of the Chlamydia genes on cell surfaces through imagining techniques and show ... Potential vaccines using gas vesicle as an antigen display can be given via the mucosal route as an alternative administration ... High concentrations of solute will cause water to be drawn out of the cell by osmosis, causing cell lysis. The ability to ... Gas vesicles occur primarily in aquatic organism as they are used to modulate the cell's buoyancy and modify the cell's ...

Alanine scanning

... scanning was used to reveal the importance of charged residues for the process of transport of the proteins to the cell surface ... This technique can also be used to determine whether the side chain of a specific residue plays a significant role in ... Cyclotides display a wide range of pharmaceutically important bioactivities, but their natural function is in plant defense as ... This technique is rapid, because many side chains are analyzed simultaneously and the need for protein purification and ...

Virus

The body's cells constantly display short fragments of their proteins on the cell's surface, and, if a T cell recognises a ... The powerful techniques developed by life sciences are becoming the basis of engineering approaches towards nanomaterials, ... If the cell has the appropriate receptor on its surface, these viruses enter the cell sometimes by direct fusion with the cell ... The causes of death include cell lysis, alterations to the cell's surface membrane and apoptosis.[122] Often cell death is ...

Eating

The brain detects insulin in the blood, which indicates that nutrients are being absorbed by cells and a person is getting full ... Some mammals are omnivores and display varying degrees of carnivory and herbivory, generally leaning in favor of one more than ... Among other practices, fasting, dieting, and vegetarianism are all techniques employed by individuals and encouraged by ... Since small mammals have a high ratio of heat-losing surface area to heat-generating volume, they tend to have high energy ...

Molecular engineering

The complexity of molecules being introduced at the surface is increasing, and the techniques used to analyze surface ... advanced fuel cells/batteries) Self-cleaning surfaces (e.g. super hydrophobic surface coatings) Flow batteries - Synthesizing ... organic light-emitting diode displays (OLED)) Electrochromic windows (e.g. windows in Dreamliner 787) Zero emission vehicles (e ... Solar cells - Developing new materials for more efficient and cost-effective solar cells including organic, quantum dot or ...

Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces | SpringerLink

... attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. ... Receptor proteins in nerve and muscle cells have ... Unable to display preview. Download preview PDF.. References. * ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. In: Kito S., Segawa T., ...

https://link.springer.com/chapter/10.1007/978-1-4757-5971-6_13

CH391L/S13/DirectedProteinEvolution - OpenWetWare

1. phage display 2. ribosome display 3. mRNA-peptide fusion 4. plasmid display 5. cell-surface display 6. genetics 7. n-hybrid ... There are a number of commercially available kits for error-prone PCR (link to Stratagene). Similar techniques also use ... Screening techniques. List of high-throughput assays for protein function ... There are several common techniques used to construct libraries of functional variants ...

https://openwetware.org/wiki/?title=CH391L/S13/DirectedProteinEvolution&curid=130741&oldid=677266

Potassium softens vascular endothelium and increases nitric oxide release | PNAS

GM7373 cells were cultured in flasks on a shaker that rhythmically tilted a thin layer of medium across the apical cell surface ... Endothelial Cell Volume and Stiffness Measurements.. Volume of living GM373 cells was determined with AFM techniques as ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Increasing Potassium Swells Endothelial Cells.. AFM imaging of living endothelial cells reveals that cell height and cell ...

http://www.pnas.org/content/106/8/2829.full

Potassium softens vascular endothelium and increases nitric oxide release | PNAS

After engagement of the cell surface with the particle tip, the cantilever will be bent as the cell is indented. A laser beam ( ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Indentation technique using AFM. (A) Cantilever with particle tip. (B) Principle of the stiffness measurement using an AFM. (C ... Time course of cortical cell stiffness measurements (means of 4 cells) in response to trypsin. The increase of cortical cell ...

https://www.pnas.org/content/106/8/2829/tab-figures-data

Toward total synthesis of cell function: Reconstituting cell dynamics with synthetic biology | Science Signaling

Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. Sci. Signal. 7, rs4 ... Introducing these constructs into inert cell lines, such as HeLa epithelial cells and Cos-7 fibroblast-like cells, these cells ... exposing the C2 domain on the cell surface so that it can bind to PS on the surface of the target apoptotic cell. Simultaneous ... Wang et al. showed that Rac activation in a single cell directed the migration of border cells, a collection of 6 to 10 cells ...

http://stke.sciencemag.org/content/9/414/re1

Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis.

25028719 - Rapidly rendering cells phagocytic through a cell surface display technique and concurr.... 20945119 - Artesunate ... of cells in the chondrocyte-like cell aggregates were positive for Scx, whereas only 3.4% of cells in the fibroblast-like cell ... Cell proliferation Ki-67 is a cell-proliferation marker [5]. In normal ACLs, 27.2% ± 4.1% of ligament cells were Ki-67 positive ... Cell density and cell arrangements The cell density in histologically normal ACLs from young donors (,45 years old) with normal ...

http://www.biomedsearch.com/nih/Cellular-extracellular-matrix-changes-in/23406989.html

Frontiers | Antibody Engineering for Pursuing a Healthier Future | Microbiology

Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ...

https://www.frontiersin.org/articles/10.3389/fmicb.2017.00495/full

IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML

... technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells ... Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...

http://www.mdpi.com/1422-0067/13/5/5420/htm

An Abundant Dysfunctional Apolipoprotein A1 in Human Atheroma - American College of Cardiology

Cell Surface Display Techniques ... Using phage display techniques, a high-affinity monoclonal ... and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo. ...

https://www.acc.org/latest-in-cardiology/journal-scans/2014/01/30/13/53/an-abundant-dysfunctional-apolipoprotein-a1-in-human

Pharmaceuticals | Free Full-Text | Aptamers for Targeted Drug Delivery | HTML

... of cell-based selection techniques has allowed selection against surface proteins in their native configuration on the cell ... All cell surface proteins cycle intracellularly to some extent, and many surface receptors are actively internalized in ... Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. ... Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking ...

http://www.mdpi.com/1424-8247/3/6/1761/htm

Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments | SpringerLink

Since the introduction of the yeast display platform, this method has increasingly gained popularity for the discovery and ... Overall, this chapter underlines the importance of the versatile yeast display technique for the optimization of the novel Fcab ... Sádio F., Stadlmayr G., Stadlbauer K., Rüker F., Wozniak-Knopp G. (2019) Yeast Surface Display and Cell Sorting of Antigen- ... Chao G, Lau WL, Hackel BJ et al (2006) Isolating and engineering human antibodies using yeast surface display. Nat Protoc 1(2): ...

https://link.springer.com/protocol/10.1007%2F978-1-4939-9024-5_13

Glypican 4 - Wikipedia

https://en.wikipedia.org/wiki/Glypican_4

Frontiers | Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System |...

We discuss the strengths and pitfalls of different techniques for anti-neuronal antibody detection: 1) Immunohistochemistry and ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... The detection of neuronal surface autoantibodies in patients serum or CSF therefore has serious consequences for the patients ...

https://www.frontiersin.org/articles/10.3389/fnmol.2016.00037/full

Use of Cxcr4 Protein Expression on the Surface of Stem Cells as a Marker for Tumor Tropic Potential - CEDARS-SINAI MEDICAL...

... and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. The ... The present invention relates to tumor tropic stem cells, ... terms may refer to the presence or display of a cell-surface ... Local intravascular administration by means of a catheter is a common technique in medical practice. For example, catheters as ... or any method which selectively binds the specific cell surface molecules. Alternatively, the method may use the cell surface ...

http://www.freepatentsonline.com/y2008/0274086.html

Patent US7843418 - Optical display system and method, active and passive dithering using ... - Google Patents

... video signal and wherein the passive display is capable of displaying a preset range of gray levels and presenting a displayed ... a light source to provide illumination of the passive display; a video signal input, wherein in response to a video signal the ... and wherein the intensity of light provided by the light source illuminating the display is controlled based on the ... passive display modulates light from the light source to provide an image, ...

http://www.google.co.uk/patents/US7843418

DiVA - Sökresultat

... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... The BACE-1 inhibitors prepared displayed IC50 values in the range of 4-45 nM, where the most potent compounds featured small P1 ... Wilkinsons catalyst [RhCl(PPh3)3] was heterogenized on common silica by the use of a grafting/anchoring technique. The ... In a preceding study we have described the development of a new hydroxyethylene (HE) core motif displaying P1 aryloxymethyl and ...

http://www.diva-portal.org/smash/resultList.jsf?dswid=-6003&p=151&fs=false&language=sv&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22631%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

DiVA - Sökresultat

... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... Here, we show that biosynthetic enzymes can display unexpected promiscuity, with human glycosyltransferase pp-a-GanT2 able to ... TEM revealed that after several catalytic runs many of the Pd nanoparticles (NPs) had migrated to the external surface of the ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...

http://www.diva-portal.org/smash/resultList.jsf?dswid=-6003&af=%5B%22hasFulltext%3Atrue%22%5D&p=1&fs=true&language=sv&searchType=SIMPLE&query=&aq=%5B%5B%7B%22organisationId%22%3A%22631%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

Ganglion Cell Physiology by Ralph Nelson - Webvision

These techniques revealed not only the ganglion cell impulses, but also the slow changes in ganglion cell membrane potential ... The arrangement of ganglion cells on the retinal surface is not spatially random. Each cell type is distributed in an ordered ... In the right column gain has been increased to better display the lower amplitude rod signals. Stimulus brightness is given in ... Y cells are called brisk transient cells, and X cells are called brisk sustained cells (Cleland and Levick, 1974a). Y cells ...

https://webvision.med.utah.edu/book/part-ii-anatomy-and-physiology-of-the-retina/ganglion-cell-physiology/

Bacterial display - Wikipedia

https://en.wikipedia.org/wiki/Bacterial_display

Method for Discovery of Peptide Reagents Using a Commercial Magnetic Separation Platform and Bacterial Cell Surface Display...

Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity ... Visit for more related articles at Journal of Analytical & Bioanalytical Techniques View PDF Download PDF ... due to sheer number of cells to sort), or how well the peptide is displayed on the cell surface. In this test case, both ... for cell sorting using yeast surface display [28-31], as well as for sorting tumor epithelial cells for downstream screening of ...

https://www.omicsonline.org/open-access/method-for-discovery-of-peptide-reagents-using-a-commercial-magneticseparation-platform-and-bacterial-cell-surface-display-technology-2155-9872-1000255.php?aid=58214

Loss of NHE8 expression impairs ocular surface function in mice | Cell Physiology

Although NHE8 displays a broad tissue distribution, it was unknown if NHE8 is expressed in the ocular surface. Here, we ... detected NHE8 expression by PCR and Western blot, and located NHE8 protein by immunohistochemistry technique. As shown in Fig. ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... only NHE1 was detected in the ocular surface epithelial cells, and its function involves cell volume regulation (47). Since ...

http://ajpcell.physiology.org/content/308/1/C79

Wang, X.<...

The use of phage display technique for the isolation of androgen receptor interacting peptides with (F/W)XXL(F/W) and FXXLY new ... Identification and Characterization of a Novel Androgen Receptor Coregulator ARA267-α in Prostate Cancer Cells. Wang, X., Yeh, ...

https://experts.umn.edu/en/persons/xin-wang

Atomic-scale observation of structural and electronic orders in the layered compound α-RuCl3 (Journal Article) | DOE PAGES

Using scanning tunnelling techniques, we observe the electronic energy gap of 0.25 eV and intra-unit cell symmetry breaking of ... RuCl3 surface layer. The corresponding charge-ordered pattern has a fine structure associated with two different types of ... real-space study by scanning transmission electron and scanning tunnelling microscopies on a novel layered material displaying ... Using scanning tunnelling techniques, we observe the electronic energy gap of 0.25 eV and intra-unit cell symmetry breaking of ...

https://www.osti.gov/pages/biblio/1337025-atomic-scale-observation-structural-electronic-orders-layered-compound-rucl3

Immunology

Using immunohistochemistry and in situ hybridization techniques to directly visualize activation markers at the cell surface, ... Once HAART is initiated, CD4 cell counts can rise but the number of cells displaying an anti-HIV response typically wanes. This ... Indolent or low-level replication in CD4 cells lacking abundant cell surface markers of activation. Haase (L9) reported that ... 269) who reported productive HIV infection of CD4+ T cells lacking markers of classic T cell activation. 3 to 19% of T cells in ...

http://www.natap.org/2002/9retro/day16.htm

SMART: NGF domain annotation

It binds as a dimer to at least 2 cell-surface receptor types expressed in a variety of neuronal and non-neuronal cells. ... Display abstract. *. Nerve growth factor (NGF), which has a tertiary structure based on a cluster of 3 cystine disulfides and 2 ... Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided ... Moreover, mast cells themselves produce NGF, suggesting that alterations in normal mast cell behaviours can provoke maladaptive ...

http://smart.embl-heidelberg.de/smart/do_annotation.pl?BLAST=DUMMY&DOMAIN=NGF

Here, we have developed a methodology for bacterial cell sorting using the semi-automated autoMACS® Pro Separator for the first time, and have produced a complete method for sorting of bacteria displaying 15-mer peptides on their cell surface using this device, including downstream bioinformatic analysis of candidates for binding to a target of interest. (omicsonline.org)
Peptides presented by human leukocyte antigen (HLA) molecules on the cell surface play a crucial role in adaptive immunology, mediating the communication between T cells and antigen presenting cells. (mcponline.org)
Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action and in cellular immunotherapy and transplantation. (mcponline.org)
Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action, the design of T-cell-mediated therapies such as tumor immunotherapy ( 1 ), and the treatment of hematological malignancies through a combination of hematopoietic stem cell transplantation and donor lymphocyte infusion ( 2 ). (mcponline.org)
Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage by fusing the peptide of interest onto gene III of filamentous phage. (wikipedia.org)

In addition to multiple implications in the regenerative potential of other tissues, mesenchymal stem cells have been shown to promote vessel formation both in vivo and in vitro. (nanohub.org)
The few in vitro studies investigating the impact of malaria on HIV-1 have demonstrated that exposure to soluble malarial antigens can increase HIV-1 infection and reactivation in immune cells. (jove.com)
Campbell A, Wicha MS, Long M (1985) Extracellular matrix promotes the growth and differentiation of murine hematopoietic cells in vitro. (springer.com)
Here, we report on the development of in vitro models of C. dubliniensis biofilms on the surfaces of biomaterials (polystyrene and acrylic) and on the characteristics associated with biofilm formation by this newly described species. (asm.org)
In vitro HIF-1α bioluminescence assay is performed by incubating the transfected cells in a hypoxic chamber (0.1% O 2 ) for 24 hr before BLI, while the cells in normoxia (21% O 2 ) serve as a control. (jove.com)
Müller cells were selectively stained with the fluorescent, calcium-sensitive dye X-Rhod-1 and were monitored in real time during retinal stretch in vitro. (arvojournals.org)

Many phage display derived binders targeting important tumor markers have been identified. (mdpi.com)
The present invention relates to tumor tropic stem cells, and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. (freepatentsonline.com)
11. The isolated stem cell of claim 8, wherein said polypeptide is tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). (freepatentsonline.com)
and assessing tumor tropic potential of the stem cell based upon said expression level of CXCR4, said affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
The investigators have demonstrated that treatment of these BRCA1/p53 conditional knockout mice with the anti-progesterone mifepristone significantly delayed the onset of mammary tumor development, reduced mammary gland morphological dysfunction, and retarded mammary epithelial cell and hyperplastic foci progression to tumors. (army.mil)
This calls for a paradigm shift in the understanding, detection, and intervention of the evolution of cancer from a single cell to tumor scale. (nanohub.org)
The technology, designed to specifically bind and enter tumor cells and the vasculature supporting tumors, is thought to be the world's largest library of human-derived targeting agents, representing thousands of unique peptide sequences. (nanowerk.com)
The development of immunotherapeutic strategies in EOC requires the identification of tumor antigens expressed in these tumors and the design of vaccination strategies that may result in the generation of tumor antigen-specific T cells that could afford durable protection. (aacrjournals.org)
To create dendritic cell vaccines, dendritic cells are removed from the patient and "force-fed" tumor antigens before being released back into the patient. (medicalnewstoday.com)
In this way, killer T cells are primed to destroy tumor cells, which are normally experts at evading the immune system. (medicalnewstoday.com)
One major drawback is that the tumor antigens used to "feed" the dendritic cells are from lab-grown tumors, not the patient's own. (medicalnewstoday.com)
Two images of EVIR-engineered dendritic cells (green) capturing tumor antigens in exosomes (gold/red). (medicalnewstoday.com)
EVIRs trap exosomes traveling through the body, giving dendritic cells the exact blueprint of the resident tumor. (medicalnewstoday.com)
Using imaging techniques, the team demonstrated that EVIRs enhanced transfer of tumor antigens from the exosome to the outer membrane of the dendritic cell. (medicalnewstoday.com)
We call this phenomenon cross-dressing, which alludes to the fact that the dendritic cells acquire immunogenic antigens from the tumor and directly display them on their own surface. (medicalnewstoday.com)
They decrease tumor cell expression of hypoxia-inducible factor 1-alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF). (jove.com)
The device delivers alternating electric fields and is programmed to ensure maximal tumor cell kill 1 . (jove.com)
Bioluminescence imaging (BLI), based on the detection of light emitted by living cells expressing a luciferase gene, has been rapidly adopted for cancer research, in particular, to evaluate tumor growth or tumor size changes in response to treatment in preclinical animal studies. (jove.com)
In the Taube lab, we are investigating the molecular pathways and cellular properties which enable primary tumor cells to metastasize. (baylor.edu)
In many pathological conditions such as metabolic diseases and tumor metastasis, cells often lose their abilities to communicate with their environment. (baylor.edu)
As CSCs are critical for tumor initiation, progression, persistence, and the development of metastasis, the success or failure of treatment approaches may be influenced greatly by the presence and treatment sensitivity of these cells. (aacrjournals.org)
Oncolytic viruses have undergone many developments and through multiple generations offer an effective way to specifically target and eradicate CSCs, while still maintaining the ability to affect the general tumor cell population. (aacrjournals.org)
As with other treatment modalities, previous oncolytic virus agents have targeted the general tumor cell population and have not specifically targeted the CSC subpopulation. (aacrjournals.org)

A simple design of a yeast-displayed heterodimeric Fc fragment is described and can be used as a general guideline for affinity selection of Fcabs with an asymmetric binding site. (springer.com)
Overall, this chapter underlines the importance of the versatile yeast display technique for the optimization of the novel Fcab scaffold for antigen recognition. (springer.com)
Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. (springer.com)
Traxlmayr MW, Lobner E, Antes B et al (2013) Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display. (springer.com)
Traxlmayr MW, Faissner M, Stadlmayr G et al (2012) Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast. (springer.com)
Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity reagents and direct amplification of bound cells without the need to elute and reinfect. (omicsonline.org)
As compared with yeast and phage display, bacterial display is ideal because of the fast doubling time of bacteria, about 20 minutes for Escherichia coli ( E. coli ) [ 7 ] versus about 2 hours for Saccharomyces cerevisiae ( S. cerevisiae ) [ 8 , 9 ], and direct amplification of the bound bacterial cells containing plasmid DNA encoding the displayed peptide responsible for binding, without elution and reinfection. (omicsonline.org)
Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. (unt.edu)
Sera from a subgroup of the patients were tested for SSX-2 and SSX-4 antibody by ELISA and recombinant antigen expression on yeast surface (RAYS). (aacrjournals.org)
Other researchers developed it decades ago, using yeast or bacterial cells, but the existing method hasn't worked to make and test diverse CDPs, Crook said. (fredhutch.org)

Granit also made the first ganglion cell recordings from a mammalian retina, the cat retina. (utah.edu)
Its distribution is highly restricted, being mostly limited to a subset of sensory hair cells in the mammalian inner ear and elsewhere to a subset of immune cells. (baylor.edu)

In the presence of aldosterone, plasma sodium in the high physiological range stiffens endothelial cells and reduces the release of nitric oxide. (pnas.org)
We now demonstrate effects of extracellular potassium on stiffness of individual cultured bovine aortic endothelial cells by using the tip of an atomic force microscope as a mechanical nanosensor. (pnas.org)
An acute increase of potassium in the physiological range swells and softens the endothelial cell and increases the release of nitric oxide. (pnas.org)
Endothelial cells are softened by estrogens through activation of a plasma membrane sodium/proton antiporter, yet are insensitive to progesterone and testosterone ( , 11 ). (pnas.org)
Similarly, as shown in renal epithelium with scanning ion conductance microscopy ( , 12 ), aldosterone stimulates endothelial cell volume, growth, and stiffness by activating epithelial sodium channels (ENaCs) ( , 13 , , 14 ). (pnas.org)
Superficial and Deep Stiffness Are Detectable in Living Endothelial Cells. (pnas.org)
Typical indentation curves of living endothelial cells are not exponential in shape. (pnas.org)
AFM imaging of living vascular endothelial cells, exposed to increasing concentrations of extracellular potassium. (pnas.org)
Indentation curves obtained in 2 experiments on vascular endothelial cells. (pnas.org)
Nitrite concentrations analyzed in the supernatant culture media after exposure of the vascular endothelial cells for 24 h to different potassium concentrations. (pnas.org)
OxTrp72-apoA1 recovered from human atheroma or plasma is lipid poor, virtually devoid of cholesterol acceptor activity, and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo . (acc.org)
Many different specialized cells exhibit these dynamic structures such as invasive cancer cells, osteoclasts, vascular smooth muscle cells, endothelial cells, and certain immune cells like macrophages and dendritic cells. (wikipedia.org)
Examples of these motile cell behaviors include: transendothelial migration of dendritic cells, migration of aortic endothelial cells for arterial vessel remodeling, and tissue infiltration by macrophages. (wikipedia.org)
Endothelial cells line the inside of blood vessels of normal organs and tumors. (unm.edu)
Here, we describe a method for differentiating, isolating, and expanding endothelial cells (ECs) from mouse embryonic stem cells (mESCs). (pubmedcentralcanada.ca)
The differentiated endothelial population expresses both Fetal Liver Kinase 1 (Flk-1) and VE-Cadherin on the cell surface which can be further purified using a fluorescence-activated cell sorting (FACS) system and subsequently expanded on 0.1 % gelatin-coated plates. (pubmedcentralcanada.ca)
Methods for developing differentiated endothelial cells from EBs are well established and the corresponding gene expression patterns are well characterized ( 4 - 6 ). (pubmedcentralcanada.ca)
Embryonic stem cells have been directly differentiated toward specific lineages without first making EBs by using conditioned medium for endothelial differentiation of ESC in collagen IV plates ( 7 , 8 ). (pubmedcentralcanada.ca)
Embryoid bodies can be directed to give rise to hemangioblasts, which subsequently undergo further differentiation into either hematopoietic or endothelial cells. (pubmedcentralcanada.ca)
After EBs are formed and exposed to endothelial differentiation medium containing VEGF, a heterogeneous cell population, including mesenchymal, hematopoietic, and epithelial cells, emerges. (pubmedcentralcanada.ca)
Only a subset of these cells will differentiate toward endothelial cells (less than 2 % with our current method). (pubmedcentralcanada.ca)
Because leukocytes are recruited to the involved myocardial region, we postulated that these activated cells would display an increased expression of adhesion molecules necessary for effective endothelial transmigration. (onlinejacc.org)

Mast cells and NGF appear to be involved in neuroimmune interactions and tissue inflammation, with NGF acting as a general 'alert' molecule capable of recruiting and priming tissue defence processes following insult as well as systemic defensive mechanisms. (embl-heidelberg.de)
This finding has led to the development and testing of second-generation small-molecule compounds that could be used clinically to inhibit cell proliferation and target Rad51 interactions. (army.mil)
they tend to spread themselves as flat as possible, have minimal cell-cell interactions and display different expression and morphogenesis. (labx.com)
It has been selected as a paradigm because the other AB5 toxin sub-families referred to above have been known for many years, and a substantial body of information on toxin-receptor interactions had been built up using conventional biochemical techniques . (functionalglycomics.org)
Such surfaces would allow for the simultaneous investigations of adhesion site maturation and composition and how changes in these properties can be implemented to engineer cell behavior via cell-surface interactions. (asme.org)
Both techniques neglect individual interactions. (hindawi.com)
This is a fascinating and unconventional route for antigen presentation to T cells, which does not require complex and rate-limiting molecular interactions inside the dendritic cell. (medicalnewstoday.com)
There is no evidence of fibronectin being associated with blood-stromal cell interactions involving either erythropoiesis or thrombopoiesis. (springer.com)

Sodium/hydrogen exchanger (NHE) 8 is expressed at the apical membrane of the epithelial cells and plays important roles in neutral sodium absorption in the gastrointestinal tract and the kidney. (physiology.org)
We also detected the expression of downregulated-in-adenoma (DRA, a Cl − /HCO 3 − transporter) in the ocular surface epithelial cells. (physiology.org)
Among them, five NHEs are detected at the plasma membrane in the epithelial cells in the intestine and the kidney. (physiology.org)
NHE1 is detected in almost all tissues and is located at the basolateral membrane in the epithelial cells ( 39 , 42 ). (physiology.org)
NHE2 and NHE3 are mainly expressed at the apical membrane in the intestinal and the renal epithelial cells ( 9 , 14 , 39 ). (physiology.org)
NHE4 is also detected in the gastric epithelial cells and renal epithelial cells ( 39 ). (physiology.org)
NHE8, the latest characterized NHE isoform, is expressed at the apical membrane in the intestinal and renal epithelial cells ( 25 , 49 ). (physiology.org)

Individual candidates were evaluated for relative binding to fluorescently-labeled PA target or streptavidin negative control using Fluorescence-Activated Cell Sorting (FACS). (omicsonline.org)
rAbs can be used in all applications in which traditional mAbs are used such as western blotting, immunohistochemistry, fluorescence-activated cell sorting (FACS), and immunofluorescence. (prolabmarketing.com)

After mixing them with the non-genetically modified lactic acid bacteria cells, the fused GFP-LcsB and Gal-LcsB were functionally associated with the cell surface of various lactic acid bacteria tested. (biomedcentral.com)
Autotransporters have been used for surface expression in Gram-negative bacteria. (diva-portal.org)
According to the researchers, one could imagine that these bacteria can carry within the bloodstream microscopic cargo, like drugs in a micro-capsule, and when triggered release this cargo at a particular site, like a cancerous or infected cell. (nanowerk.com)
Our analysis shows that a cell component derived from bacteria appears to stimulate egg laying, a serotonin-dependent reproductive behavior, while it also stimulates the movement of worms. (baylor.edu)
Although ovalbumin-expressing bacteria are being used to trace T cell responses to colonizing or invading pathogens, current constructs for ovalbumin presentation have not been optimized. (biomedcentral.com)

Using molecular techniques the researchers investigated how the antibody was protecting mice. (www.nhs.uk)
It is possible to perform all of these techniques in a standard molecular biology lab or the process can be outsourced to a variety of facilities specializing in the generation of rAbs. (jhsph.edu)
To circumvent these problems, researchers including British biochemist Greg Winter invented robust "molecular display" technologies. (harvard.edu)
By doing so, Jonkheijm, who heads research into Bioinspired Molecular Engineering, and his team, were able to show that living cells can also be used as a component in supramolecular smart materials. (nanowerk.com)
Mechanical stress triggers molecular responses in Müller cells that could prevent retinal damage. (arvojournals.org)

Their protocol for Creation of Baculovirus Display Libraries is featured, and is freely available on the journal's website ( cshprotocols.cshlp.org/cgi/content/full/2010/3/pdb.prot5393 ). (cshlpress.com)
With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. (creative-biolabs.com)
Antibody libraries are constructed by the genomic information coding for antibody variable domains, which can be derived from B cells of immune or naïve donors. (creative-biolabs.com)
We have addressed this issue for two intimately related serine proteases, tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (uPA), by modifying the technique of substrate phage display to create substrate subtraction libraries. (elsevier.com)
A patent by George Pieczenik claiming priority from 1985 also describes the generation of phage display libraries. (wikipedia.org)

The surface expression of enzymes and vaccine epitopes offer several advantages. (diva-portal.org)
Surface expressed vaccine epitopes can have longer half-lives inside the animal that is to be immunized and surface groups on the host cell can act as adjuvants, increasing the immune response and leading to a better immunisation. (diva-portal.org)
These HLA ligands can form the basis for reversed immunology approaches to identify T cell epitopes based not on in silico predictions but on the bona fide eluted HLA ligandome. (mcponline.org)

Peptide candidates produced with this method contained the previously reported PA consensus WXCFTC, further validating this method and the commercially available autoMACS® platform as the first low cost, semi-automated biopanning approach for bacterial display that is widely accessible and more reliable than the MACS/FACS standard protocol. (omicsonline.org)

E. coli displaying multiple enzymes. (igem.org)
Then, the cDNA clones encoding variable heavy and variable light chains of these four mabs were isolated, and used for construction of four single-chain Fv (scFv) antibody genes, which were expressed in Escherichia coli cells. (go.jp)
Additionally, we want to make one of these aptamers bind to the E. coli cell surface. (openwetware.org)
What aptamers exist that bind the E. coli cell surface? (openwetware.org)
The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into Escherichia coli (E. coli) bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)

5. The isolated stem cell of claim 1, wherein said isolated stem cell comprises a heterologous gene. (freepatentsonline.com)
6. The isolated stem cell of claim 5, wherein said heterologous gene encodes a polypeptide of therapeutic use in the treatment of a disease condition. (freepatentsonline.com)
Furthermore, to improve upon current in vivo gene delivery methods, COE investigators have engineered specialized expression vectors with cancer-specific promoters, a two-step transcriptional activation system, and post-translational regulatory elements that are capable of expressing therapeutic genes by several hundred-fold in breast cancer cells only. (army.mil)
Each variable region gene is spliced into a vector (vehicles that transfer foreign genetic material into another cell), and the vector is taken up and integrated into the genome of a microorganism. (jhsph.edu)
a ) predominant expression in germ cells of the testis and generally not in other normal tissues, ( b ) expression in a proportion of malignant tumors of different histologic types, ( c ) expression in malignancies in a lineage nonspecific fashion, ( d ) often mapping of the gene on the X chromosome, ( e ) often members of multigene families. (aacrjournals.org)
In the March issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc3_10.dtl ), Christian Oker-Blom and colleagues ( www.jyu.fi/bio/bit/tutkimus_files/research.html ) provide a suite of articles detailing the use of baculovirus-based display and gene delivery systems. (cshlpress.com)
Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. (creative-biolabs.com)
Using a gene editing technique, the CRISPR-CAS9 system, we are generating mutations in candidate genes and characterizing genetic or biochemical links to integrin. (baylor.edu)

Bacterial display sorting is a powerful emerging technology that offers a rapid, high-throughput approach to discovery of robust affinity reagents [ 1 - 3 ]. (omicsonline.org)
The team has previously used an advanced high-throughput screening technique called phage display to discover a small peptide that ultimately was chemically modified to become Vasotide. (unm.edu)
In addition, the technique can be scaled up for the high-throughput approaches of genomics and proteomics. (harvard.edu)

However, these studies used whole cell extracts of P. falciparum schizont stage parasites and peripheral blood mononuclear cells (PBMC), making it hard to decipher which malarial component(s) was responsible for the observed effects and what the target host cells were 4,5 . (jove.com)

Altered ocular surface function and reduced epithelial DRA expression in NHE8−/− mice suggest that the role of NHE8 in ocular surface tissue involve in tear production and ocular epithelial protection. (physiology.org)
Consequently, podosomes are present in cell types associated with tissue remodeling and the immune system. (wikipedia.org)
Moreover, mast cells themselves produce NGF, suggesting that alterations in normal mast cell behaviours can provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states. (embl-heidelberg.de)
Stem cells have demonstrated remarkable potential for tissue engineering and regenerative medicine through their ability for self-renewal and controlled differentiation. (nanohub.org)
Bentley SA, Foidart J-M (1980) Some properties of marrow derived adherent cells in tissue culture. (springer.com)

SubB has recently been shown to bind to N-linked glycans displayed on several glycoproteins on the surface of Vero and HeLa cells, including α2β1integrin . (functionalglycomics.org)
Each ShuA variant was analyzed for its ability to display on the bacteriophage surface, and functionally bind to hemoglobin. (rsc.org)

J. Cell Biol. (wikipedia.org)
Curr Opin Cell Biol. (jhmi.edu)

Through an in vivo approach, the genotype-phenotype link is produced by using a living cell. (hindawi.com)
3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (labx.com)
Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (labx.com)
Consequently, administration of anti-CD25 Abs is a commonly used technique to deplete Treg populations in vivo. (nih.gov)

In 1839, at age 19, he built the world's first photovoltaic cell in his father's laboratory. (wikipedia.org)
To address these issues, he and his laboratory members take a combined biochemical, cell biological, and genetic approach. (cancer.gov)
Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. (asm.org)
ICON is all about transitioning scalable nanoimprinting techniques out of the laboratory and into the marketplace. (nanotech-now.com)
The Simmons laboratory also investigates how specialized sensory cells and their innervation patterns are refined by calcium signals during early stages of development. (baylor.edu)
This laboratory technique uses an antibody conjugated with a fluorochrome for cell analysis. (news-medical.net)

Two autoMACS® programs designed for isolation of target cells with low frequency were evaluated and adapted to bacterial biopanning, using protective antigen (PA) of Bacillus anthracis as the model system. (omicsonline.org)
This technique streamlines isolation of stable MP variants for rapid characterization of binding to various ligands. (rsc.org)

Using phage display techniques, a high-affinity monoclonal antibody was developed that specifically recognizes both apolipoprotein A1 (apoA1) and HDL that have been modified by the MPO-H 2 O 2 -Cl− system. (acc.org)

Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided sufficient material to enable its biochemical characterization. (embl-heidelberg.de)
Akiyama SK, Yamada SS, Yamada KM (1986) Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule. (springer.com)
Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, McKenzie S, Broxmeyer HE, Moore MAS (1980) Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (springer.com)
Mitochondria are heterogeneous organelles involved in energy production, metabolism, and cellular signaling that oftentimes are isolated from cells for chemical characterization (e.g., proteomic analysis). (biomedsearch.com)

In contrast to manual MACS, the bacterial display library was preferentially enriched by autoMACS® sorting, yielding several promising candidates after only three rounds of biopanning and bioinformatic analysis. (omicsonline.org)

Nevertheless, performance is limited by non-radiative losses, with luminescence yields in state-of-the-art perovskite solar cells still far from 100 per cent under standard solar illumination conditions. (diva-portal.org)
When interfaced with electrodes in a solar cell device stack, the external luminescence yield-a quantity that must be maximized to obtain high efficiency-remains as high as 15 per cent, indicating very clean interfaces. (diva-portal.org)
We validate these results in fully operating solar cells. (diva-portal.org)
For convection cells on the moon's surface, see Granule (solar physics) . (wikipedia.org)
A conventional crystalline silicon solar cell (as of 2005). (wikipedia.org)
A solar cell , or photovoltaic cell , is an electrical device that converts the energy of light directly into electricity by the photovoltaic effect , which is a physical and chemical phenomenon. (wikipedia.org)
Individual solar cell devices can be combined to form modules, otherwise known as solar panels . (wikipedia.org)
In basic terms a single junction silicon solar cell can produce a maximum open-circuit voltage of approximately 0.5 to 0.6 volts. (wikipedia.org)
Solar cells are described as being photovoltaic , irrespective of whether the source is sunlight or an artificial light. (wikipedia.org)
A "photoelectrolytic cell" ( photoelectrochemical cell ), on the other hand, refers either to a type of photovoltaic cell (like that developed by Edmond Becquerel and modern dye-sensitized solar cells ), or to a device that splits water directly into hydrogen and oxygen using only solar illumination. (wikipedia.org)
From a solar cell to a PV system. (wikipedia.org)
Multiple solar cells in an integrated group, all oriented in one plane, constitute a solar photovoltaic panel or module . (wikipedia.org)
Solar cells are usually connected in series and parallel circuits or series in modules, creating an additive voltage. (wikipedia.org)
Research into applications for carbon graphene nanosheets has focused on uses as platforms for next-wave microchips, active materials in field emitter arrays for flat panel screen displays, in biological sensors and medical imaging devices, in solar energy cells, and in high-surface area electrodes for use in bio-science. (americanelements.com)
The nano electronic elements can include solar cells, display elements, or antennas, among othe. (google.com)
This manufacturing process can easily and quickly mass produce films and surfaces with nanometer-scale textures for a host of new applications in biomedical devices, optical films, plastic electronics and flexible solar cells. (nanotech-now.com)
1. Nanoimprinted structures and components are being used in items such as anti-reflection films, and solar cells. (nanotech-now.com)
Some of the applications that the consortium hopes to harness with roll-to-roll nanoimprint include anti-fouling surfaces, anti-reflection films to enhance the efficiency of solar cells, wire-grid polarisers, and optical films for flat panel displays. (nanotech-now.com)
7. "This partnership on roll-to-roll nanoimprinting enhances our competitiveness in the global arena, particularly in helping us create new functional and patterned film technologies for displays and solar cell applications", said Mr Kim Woo Yong, Global Marketing Director of Young Chang Chemical Co. Ltd, South Korea. (nanotech-now.com)

While some advances have been made by other groups where supramolecular elements were introduced on cell membranes by merging artificially made membranes, this is the first time where the cells were made to produce and display supramolecular elements on their surfaces through genetic engineering. (nanowerk.com)

Megakaryocyte (MK) progenitor cells proliferate, polyploidize, increase in size, and develop lineage-specific cell surface and cytoplasmic markers. (bloodjournal.org)

Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. (frontiersin.org)
Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. (mdpi.com)
However, widespread use is limited, in part due to poor performance achieved using manual Magnetic-Activated Cell Sorting (MACS) methods, and an absence of widely-available, low cost, high-performance sorting alternatives. (omicsonline.org)
Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. (unt.edu)
These results demonstrate that the combination of substrate phage display and substrate subtraction methods can be used to discover specificity differences between very closely related enzymes and that this information can be utilized to create highly selective inhibitors. (elsevier.com)
Although ultimately, the rewards of targeting and eradicating CSCs will be evaluated in clinical trials, there are numerous methods for isolating primary CSCs based on surface marker expression and multiple established cell lines representative of CSCs for preliminary evaluation. (aacrjournals.org)

The stem cells may additionally exhibit markers characteristic of astrocytic progenitors. (freepatentsonline.com)
The stem cells may be administered as part of a treatment regimen including the chemokine SDF-1. (freepatentsonline.com)
2. The isolated stem cell of claim 1, wherein said isolated stem cell exhibits markers characteristic of a precursor for astrocytic differentiated stem cells. (freepatentsonline.com)
3. The isolated stem cell of claim 2, wherein said isolated stem cell exhibits an A2B5 astrocytic precursor marker. (freepatentsonline.com)
7. The isolated stem cell of claim 6, wherein said polypeptide is cytotoxic. (freepatentsonline.com)
8. The isolated stem cell of claim 6, wherein said polypeptide is involved in an immune response. (freepatentsonline.com)
9. The isolated stem cell of claim 8, wherein said polypeptide is IL-12. (freepatentsonline.com)
12. The isolated stem cell of claim 1, wherein said isolated stem cell is a neural stem cell (NSC). (freepatentsonline.com)
14 . The method of claim 14, wherein said stem cell exhibits an A2B5 astrocytic precursor marker. (freepatentsonline.com)
17. The method of claim 14, wherein said stem cell is a neural stem cell (NSC). (freepatentsonline.com)
4 5 Although thrombopoietin (TPO) plays a major role in regulating MK and platelet production, 6-8 many studies point to additional contributions from stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), interleukin-11 (IL-11), granulocyte-macrophage colony-stimulating factor (GM-CSF), and basic fibroblast growth factor (bFGF). (bloodjournal.org)
Cancer stem cells (CSC) represent a distinct subpopulation of cancer cells of integral importance. (aacrjournals.org)
This treatment resistant subpopulation still lacks full definition but has been characterized as cancer-initiating cells or cancer stem (or stem-like) cells (CSC). (aacrjournals.org)
This subpopulation will be referred to herein as cancer stem cells (CSCs). (aacrjournals.org)

Bentley SA, Tralka TS (1983) Fibronectin-mediated attachment of hematopoietic cells to stromal elements in continuous bone marrow culture. (springer.com)

The detection of neuronal surface autoantibodies in patients' serum or CSF therefore has serious consequences for the patients' treatment and follow-up and requires the availability of sensitive and specific diagnostic tests. (frontiersin.org)
To achieve this, the availability of sensitive and specific tests to detect cell-surface autoantibodies is of key importance. (frontiersin.org)
Ganglion cell axons are directed to specific visual centers depending of the visual 'trigger features' they encode. (utah.edu)
The production of interferon gamma by T cells in response to a particular antigen is proof of a pre-existing mature and specific immune response. (natap.org)
Such cells control their degree of innervation (the number of axon connections) by the secretion of various specific neurotrophic factors that are essential for neuron survival. (embl-heidelberg.de)
AB5 toxins are an important family of bacterial toxins, so termed because they comprise a catalytic A subunit, non-covalently linked to a pentameric B subunit that binds to specific host cell surface glycans. (functionalglycomics.org)
Surface modifications of the viral capsid enable specific targeting. (cshlpress.com)
Significant quantities of Env-specific CD4+ T cells were also detected, which were augmented as much as 70-fold by priming with IM electroporated plasmids encoding EnvG and IL-12. (jove.com)
Immunologists have been using ovalbumin as a reporter antigen for years and have developed sophisticated tools to detect specific T cells that respond to ovalbumin. (biomedcentral.com)
Since endogenous T cells specific for any given antigen are present in small numbers, it makes the tracking of such cells difficult during the early phases of an immune response before clonal expansion has occurred. (biomedcentral.com)

Scientists have discovered that a type of immune cell that normally helps fight infection may actually assist lung tumors. (medicalnewstoday.com)
important in a number of processes, such as coagulation, cell signaling, and waste management, they are produced by tumors in large amounts. (medicalnewstoday.com)
Notably, a subpopulation of cancer cells that are often resistant to conventional treatment regimens and are responsible for the initiation of tumors in xenograft models have recently been discovered and characterized. (aacrjournals.org)

Patients who suffer from Wiskott-Aldrich Syndrome demonstrate, through their immune cells, continued evidence of the role podosomes fulfill in cell motility. (wikipedia.org)

In this paper we present the in-depth identification and relative quantification of 14,500 peptide ligands constituting the HLA ligandome of B cells. (mcponline.org)

ribosome display 3. (openwetware.org)

Acute changes in sodium or potassium affect only the first slope that is produced by the cell's shallow outer shell and leave the second slope produced by the inner part of the cell unchanged. (pnas.org)
In 1888 Russian physicist Aleksandr Stoletov built the first cell based on the outer photoelectric effect discovered by Heinrich Hertz in 1887. (wikipedia.org)

Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes. (1/81)

Relevant uses of surface proteins--display on self-organized biological structures. (2/81)

SplitCore: an exceptionally versatile viral nanoparticle for native whole protein display regardless of 3D structure. (3/81)

Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display. (4/81)

Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin. (5/81)

Bacillus subtilis: a temperature resistant and needle free delivery system of immunogens. (6/81)

Baculovirus as a vaccine vector. (7/81)

Surface display of N-terminally anchored invasin by Lactobacillus plantarum activates NF-kappaB in monocytes. (8/81)

Glypican 4

Podosome

Nutritional genomics

Luciferase

Solar cell

Bacterial display

Hidden surface determination

Yeast display

B-1 cell

Ectomycorrhiza

Microbead (research)

Screen printing

Atomic force microscopy

Immunohistochemistry

Vaa3D

Lipid bilayer

Immunohistochemistry

Axon guidance

Screen printing

Human genetic resistance to malaria

Pixel

Phage display

Protein engineering

Gas vesicle

Alanine scanning

Virus

Eating

Molecular engineering

Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces | SpringerLink

CH391L/S13/DirectedProteinEvolution - OpenWetWare

Potassium softens vascular endothelium and increases nitric oxide release | PNAS

Potassium softens vascular endothelium and increases nitric oxide release | PNAS

Toward total synthesis of cell function: Reconstituting cell dynamics with synthetic biology | Science Signaling

Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis.

Frontiers | Antibody Engineering for Pursuing a Healthier Future | Microbiology

IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML

An Abundant Dysfunctional Apolipoprotein A1 in Human Atheroma - American College of Cardiology

Pharmaceuticals | Free Full-Text | Aptamers for Targeted Drug Delivery | HTML

Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments | SpringerLink

Glypican 4 - Wikipedia

Frontiers | Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System |...

Use of Cxcr4 Protein Expression on the Surface of Stem Cells as a Marker for Tumor Tropic Potential - CEDARS-SINAI MEDICAL...

Patent US7843418 - Optical display system and method, active and passive dithering using ... - Google Patents

DiVA - Sökresultat

DiVA - Sökresultat

Ganglion Cell Physiology by Ralph Nelson - Webvision

Bacterial display - Wikipedia

Method for Discovery of Peptide Reagents Using a Commercial Magnetic Separation Platform and Bacterial Cell Surface Display...

Loss of NHE8 expression impairs ocular surface function in mice | Cell Physiology

Wang, X.<...

Atomic-scale observation of structural and electronic orders in the layered compound α-RuCl3 (Journal Article) | DOE PAGES

Immunology

SMART: NGF domain annotation

Peptides5

Vitro6

Tumor23

Yeast10

Mammalian2

Endothelial22

Interactions8

Epithelial cells7

Fluorescence-activated cell2

Bacteria5

Molecular5

Libraries5

Epitopes3

FACS1

Coli5

Gene8

High-throughput3

Peripheral blood mononuclear cells1

Tissue5