Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Endosseous dental implantation where implants are fitted with an abutment or where an implant with a transmucosal coronal portion is used immediately (within 1 week) after the initial extraction. Conventionally, the implantation is performed in two stages with more than two months in between the stages.
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The fifth planet in order from the sun. It is one of the five outer planets of the solar system. Its sixteen natural satellites include Callisto, Europa, Ganymede, and Io.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Viruses whose hosts are bacterial cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS.
Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Compounds that contain the radical R2C=N.OH derived from condensation of ALDEHYDES or KETONES with HYDROXYLAMINE. Members of this group are CHOLINESTERASE REACTIVATORS.
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Standards for limiting worker exposure to airborne contaminants. They are the maximum concentration in air at which it is believed that a particular substance will not produce adverse health effects with repeated daily exposure. It can be a time-weighted average (TLV-TWA), a short-term value (TLV-STEL), or an instantaneous value (TLV-Ceiling). They are expressed either as parts per million (ppm) or milligram per cubic meter (mg/m3).
The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)
An institute of the CENTERS FOR DISEASE CONTROL AND PREVENTION which is responsible for assuring safe and healthful working conditions and for developing standards of safety and health. Research activities are carried out pertinent to these goals.
Exclusive legal rights or privileges applied to inventions, plants, etc.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The visual display of data in a man-machine system. An example is when data is called from the computer and transmitted to a CATHODE RAY TUBE DISPLAY or LIQUID CRYSTAL display.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Information systems, usually computer-assisted, designed to store, manipulate, and retrieve information for planning, organizing, directing, and controlling administrative activities associated with the provision and utilization of radiology services and facilities.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
A chemical element having an atomic weight of 106.4, atomic number of 46, and the symbol Pd. It is a white, ductile metal resembling platinum, and following it in abundance and importance of applications. It is used in dentistry in the form of gold, silver, and copper alloys.
Any chemical species which accepts an electron-pair from a LEWIS BASE in a chemical bonding reaction.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Changing an open-chain hydrocarbon to a closed ring. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Unsaturated hydrocarbons of the type Cn-H2n, indicated by the suffix -ene. (Grant & Hackh's Chemical Dictionary, 5th ed, p408)
Neurons of the innermost layer of the retina, the internal plexiform layer. They are of variable sizes and shapes, and their axons project via the OPTIC NERVE to the brain. A small subset of these cells act as photoreceptors with projections to the SUPRACHIASMATIC NUCLEUS, the center for regulating CIRCADIAN RHYTHM.
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
A syndrome characterized by HYPERPIGMENTATION, enlarging pituitary mass, visual defects secondary to compression of the OPTIC CHIASM, and elevated serum ACTH. It is caused by the expansion of an underlying ACTH-SECRETING PITUITARY ADENOMA that grows in the absence of feedback inhibition by adrenal CORTICOSTEROIDS, usually after ADRENALECTOMY.
INTERNEURONS of the vertebrate RETINA. They integrate, modulate, and interpose a temporal domain in the visual message presented to the RETINAL GANGLION CELLS, with which they synapse in the inner plexiform layer.
Photosensitive afferent neurons located primarily within the FOVEA CENTRALIS of the MACULA LUTEA. There are three major types of cone cells (red, blue, and green) whose photopigments have different spectral sensitivity curves. Retinal cone cells operate in daylight vision (at photopic intensities) providing color recognition and central visual acuity.
Photosensitive proteins expressed in the ROD PHOTORECEPTOR CELLS. They are the protein components of rod photoreceptor pigments such as RHODOPSIN.

Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes. (1/81)

 (+info)

Relevant uses of surface proteins--display on self-organized biological structures. (2/81)

 (+info)

SplitCore: an exceptionally versatile viral nanoparticle for native whole protein display regardless of 3D structure. (3/81)

 (+info)

Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display. (4/81)

 (+info)

Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin. (5/81)

 (+info)

Bacillus subtilis: a temperature resistant and needle free delivery system of immunogens. (6/81)

 (+info)

Baculovirus as a vaccine vector. (7/81)

 (+info)

Surface display of N-terminally anchored invasin by Lactobacillus plantarum activates NF-kappaB in monocytes. (8/81)

 (+info)

The unique property of specific high-affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. Hence knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. Here, we describe a high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen fragments followed by antibody-based flow cytometric sorting. Epitopes are determined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. The method described here has been useful for the mapping of both monoclonal and polyclonal antibodies with varying sizes of epitopes.. ...
National Pingtung University of Science and Technology - Faculty Open Information System. | 國立屏東科技大學教師資訊公開查詢系統,提供教師個人、授課、著作及研發績效等資訊查詢
A new technique, dubbed prime editing, appears to make it even easier to make very precise changes in DNA. Its designed to overcome the limits of the CRISPR gene editing tool.
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins.. Affinity proteins used in therapeutic and diagnostic applications commonly aim to specifically bind to disease-related drug targets. Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a critical process in various types of cancer and vascular eye disorders. Vascular Growth Factor Receptor 2 (VEGFR2) is one of the main regulators of angiogenesis. The first two studies presented in this thesis ...
A biorefinery is a special type of refinery in which biomass, such as plant cellulose, is converted by microorganisms into useful products. Edinburghs 2011 iGEM project is a feasibility study into the creation of biorefineries using E. coli, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create synergy between them. We investigated two methods of bringing the enzymes close together: cell surface display via Ice Nucleation Protein, and phage display via M13s major coat protein. We attempted a new DNA assembly protocol, provisionally named BioSandwich. We constructed computer models of synergy to assess whether it is a feasible option. Finally, we considered the broader economic and social questions surrounding the construction of a biorefinery: can it be done, and should it be done? ...
TY - JOUR. T1 - Automated panning and screening procedure on microplates for antibody generation from phage display libraries. AU - Turunen, Laura. AU - Takkinen, Kristiina. AU - Söderlund, Hans. AU - Pulli, Timo. PY - 2009. Y1 - 2009. N2 - Antibody phage display technology is well established and widely used for selecting specific antibodies against desired targets. Using conventional manual methods, it is laborious to perform multiple selections with different antigens simultaneously. Furthermore, manual screening of the positive clones requires much effort. The authors describe optimized and automated procedures of these processes using a magnetic bead processor for the selection and a robotic station for the screening step. Both steps are performed in a 96-well microplate format. In addition, adopting the antibody phage display technology to automated platform polyethylene glycol precipitation of the enriched phage pool was unnecessary. For screening, an enzyme-linked immunosorbent assay ...
Phage display technology for identifying selective antigens and address molecules on brain endothelial cells. In Blood Barrier: Biology and Research Protocols (Ed. S. Nag)
Creative Biolabs is one of the well-recognized expert who is professional in applying various phage display technologies to offer library construction and screening related services for a broad range of project objectives.
Healing From The Cause uses a unique and powerful technique called Holographic Kinetics. It enables issues to be fully cleared at their cause.
Healing From The Cause uses a unique and powerful technique called Holographic Kinetics. It enables issues to be fully cleared at their cause.
Based on years of experience in phage display technology, Creative Biolabs now is able to provide services for the construction and screening of immune antibody library.. With years of research and development in the past decades, phage display has been a powerful technology to display millions or even billions of different peptides or proteins. It is now a common choice for the studies of protein-protein, protein-peptide and protein-DNA interactions. Among all the applications of phage display technology, one of the most successful ones is the isolation of monoclonal antibodies utilizing large capacity phage antibody libraries.. Thanks to the rapid development of this technology, it has become a reliable tool for the production of monoclonal antibody with high specificity and affinity. Compared with conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners. This expands the possibility to develop monoclonal antibodies ...
Having been researching in the field of phage display technology for years, Creative Biolabs is able to offer a series of comprehensive phage display library screening services by its own biopanning strategies and enhanced equipments.. Phage display technology is one of the major approaches applied in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds and ligands. As binders with the desired specificity generally exist at low frequencies in the constructed libraries, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.. Libraries from customized library construction services, in-house premade antibody libraries, peptide libraries or scaffold libraries, libraries provided by clients and other commercialized libraries can all be screened in Creative Biolabs.. No matter its purified or unpurified targets, we can screen for ...
INTRODUCTION Cancer of the testes is currently the most frequent neoplasm and a leading cause of morbidity in men 15-35 years of age. Its incidence is increasing. Embryonal carcinoma is its most malignant form, which either may be resistant or may develop resistance to therapies, which results in relapses. Cancer stem cells are hypothesized to be drivers of these phenomena. SPECIFIC AIM The specific aim of this work was identification and isolation of spectra of single, living cancer stem cells, which were acquired directly from the patients biopsies, followed by testing of their pluripotency. PATIENTS METHODS Biopsies were obtained from the patients with the clinical and histological diagnoses of the primary, pure embryonal carcinomas of the testes. The magnetic and fluorescent antibodies were genetically engineered. The SSEA-4 and TRA-1-60 cell surface display was analyzed by multiphoton fluorescence spectroscopy (MPFS), flow cytometry (FCM), immunoblotting (IB), nuclear magnetic resonance
Paroles de boll weevil chanson de the presidents of the usa? Trouvez sur www.parolesbox.fr toutes les paroles de the presidents of the usa dont la musique boll weevil
Buy Eddie Cochran - Somethin Else / Boll Weevil Song - Liberty - 7, Single, Promo - F-55203, includes Somethin Else, Boll Weevil Song
Buy Eddie Cochran - Somethin Else / Boll Weevil Song - Liberty - 7, Single, Promo - F-55203, includes Somethin Else, Boll Weevil Song
Boll Weevil is a minor antagonist from the TV series DuckTales. He is an evil, ruthless, anthropomorphic dog and a greedy oat tycoon and farmer. He is the main villain of the episode "Horse Scents." Boll Weevil first appears after Mr. Merriweathers camera breaks in an untimely accident, and he...
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described
A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these...
In 1915, Floridas agricultural economy was in serious trouble. Its principal cash-crop, cotton, was being devastated by the boll weevil, an invasive beetle that crossed over from Mexico in the early 1900s. Farmers were desperate to make up… Read More. ...
EXECUTIVE INN & SUITES in Enterprise AL at 615 Boll Weevil 36330 US. Check reviews and discounted rates for AAA/AARP members, seniors, groups & military/govt.
Last night for dinner we went to Boll Weevil for the best deal in town. All you can eat fish fry for only $7.99. With coupon its only $5.99! Hard to believe, I know. And you can tell its good quality fish, not processed institutional patties. Includes a salad, oddly served in a wooden bowl.…
VacantLand property for sale in Enterprise,AL (MLS #465572). Learn more from Team Linda Simmons Real Estate. GREAT COMMERCIAL LAND WITH DEEP LOTS.
These terms and conditions apply between you, the User of this Website (including any sub-domains, unless expressly excluded by their own terms and conditions), and KEVOM LT, the owner and operator of this Website. Please read these terms and conditions carefully, as they affect your legal rights. Your agreement to comply with and be bound by these terms and conditions is deemed to occur upon your first use of the Website. If you do not agree to be bound by these terms and conditions, you should stop using the Website immediately.. In these terms and conditions, User or Users means any third party that accesses the Website and is not either (i) employed by KEVOM LT and acting in the course of their employment or (ii) engaged as a consultant or otherwise providing services to KEVOM LT and accessing the Website in connection with the provision of such services.. You must be at least 18 years of age to use this Website. By using the Website and agreeing to these terms and conditions, you represent ...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells surfaces lead to the identification of unknown tumoral markers. Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. Many Ab molecules directly or indirectly targeting
Data from a survey were used to evaluate Tennessee farmers willingness to pay for the boll weevil eradication program. Producer experience, boll weevil control costs, and attitudes about boll weevil damage and insecticide usage after the program were significant explanatory variables and had a positive influence on willingness to pay. Larson, James A.; Collins, Rebecca L.; Roberts, Roland K.; English, Burton C.
Get information, facts, and pictures about boll weevil at Encyclopedia.com. Make research projects and school reports about boll weevil easy with credible articles from our FREE, online encyclopedia and dictionary.
Cole Sears 10-yard touchdown run with 1:44 remaining sent the University of Arkansas-Monticello to a 46-45 Great American Conference win over nationally 24th-ranked Arkansas Tech University at Convoy Leslie Cotton Boll Stadium in Monticello on Saturday.. Josh Marini added the extra point following Sears scamper for the ninth and final lead change of the day.. Arkansas Tech (4-2 overall, 4-2 GAC) turned the ball over on downs at its own 39-yard line with 1:11 remaining and the Boll Weevils (3-3, 3-3) ran out the clock.. The first half alone saw five lead changes and a combined 492 yards of total offense.. Techs Ty Reasnor and UAMs Sears both threw a touchdown pass in the first quarter to forge a 7-7 tie after 15 minutes.. The Boll Weevils took their first lead of the day on a 1-yard touchdown run by Jalen Tolliver at the 10:43 mark of the second quarter. The extra point try was no good, leaving UAM with a 13-7 edge.. Justin Bailey scored his first career rushing touchdown as a Wonder Boy and ...
Buy and Sell Arkansas-Monticello Boll Weevils Mens Basketball tickets with no fees! Check the schedule, watch highlights, read news and team stats at CHARGED.fm
The Boll Weevil, cotton in Texas, and Tex Ritter; by Archie McDonald, PhD., All Things Historical, an East Texas syndicated historical column.
Texas Boll Weevil Eradication located at Abilene, TX - find the revenue, phone#, the employees list and their detailed information including the job titles and emails, and much more on LeadFerret.com.
I met Kathy trying to sell our first house. It had been on the market with another agent and it just wasnt being looked at. After six months or more I found Kathy and she helped me stage my home. Within two weeks we began to have numerous viewings and... (more). I met Kathy trying to sell our first house. It had been on the market with another agent and it just wasnt being looked at. After six months or more I found Kathy and she helped me stage my home. Within two weeks we began to have numerous viewings and quickly sold our home. She was very helpful in explaining to my three young sons the importance of keeping their rooms clean and supporting the get the house ready to be viewed concept. She reminded them that we were selling this house to move into our new home out of state and be reunited with their dad when he came home from being deployed. It worked, they got so good at it that I could just call them from work and tell them that someone was coming to look at the house, to clean it up ...
Listing Agent: Jackie Thompson. Courtesy of: CENTURY 21 Regency Realty, Inc.. Office Phone: 3343470048. Updated: 22nd January, 2018 5:34 AM.. ...
Jack Dailey, a former student from my teaching days, checks in with Part 1 of the story of his family farm in Louisiana, and he sends us a killer recipe for spatchcocked Cornish game hens, which taste much better once you know what spatchcocked actually means.. ...
Jack Dailey, a former student from my teaching days, checks in with Part 1 of the story of his family farm in Louisiana, and he sends us a killer recipe for spatchcocked Cornish game hens, which taste much better once you know what spatchcocked actually means.. ...
VacantLand property for sale in Enterprise,AL (MLS #174710). Learn more from Team Linda Simmons Real Estate. Next to State Employment Center, close to High School.
Dear Thor!, I exclaimed. I had solved the most annoying problems in DNA chemistry in a single lightening bolt. Abundance and distinction. With two oligonucleotides, DNA polymerase, and the four nucleosidetriphosphates I could make as much of a DNA sequence as I wanted and I could make it on a fragment of a specific size that I could distinguish easily. Somehow, I thought, it had to be an illusion. Otherwise it would change DNA chemistry forever. Otherwise it would make me famous. It was too easy. Someone else would have done it and I would surely have heard of it. We would be doing it all the time. What was I failing to see? Jennifer, wake up. Ive thought of something incredible. --Kary Mullis from his Nobel lecture; December 8, 1983 The PCR primers you will use today recognize sequences flanking the Aga2 fusion on the yeast display plasmid. After performing PCR, the DNA produced will be mixed with yet another primer that will serve as a starting point for the sequencing reactions. ...
The project „BiCleanuses fingerprint sensors as an example, offering an innovative technical tool for the detection and removal of biofilms and thus preventing the risk of the transmission of pathogenic germs. Bidirectional displays have been coated with titanium dioxide (TiO2) or TiO2-containing layer combinations, thus the biofilm can be inactivated.. Bidirectional displays can emit light or content as well as detect and evaluate the incoming light via an integrated camera function. The development of this display technology is one of the core competencies of the Fraunhofer FEP. The bidirectional characteristic allows the recording of the surface status by the device itself.. This makes it possible to detect the formation of biofilms and then start a cleaning interval depending on the degree of contamination. Another research focus is the cleaning of the surface, which will also be done by light emission from the display. This is where another core competence of the Fraunhofer FEP comes ...
Feature: In the second part of our in-depth LCoS investigation, youll learn about or test methodology and equipment, and well ...
Some of you may be wondering exactly how Electroluminescent Display Technology can be used for your application. It seems the folks at EF Johnson Technologies have
NOTE: this is a long post full of a-ha tips and powerful techniques that will skyrocket your weight loss and fitness results. Click here to download the
Id like to update you on the progress of my Agency, the U.S. Department of Agricultures Animal and Plant Health Inspection Service, and our partners in the States and cotton industry have made towards completely eradicating boll weevil from the United States. More than 30 years ago, you could find boll weevils-a tiny, long-snouted, persistent pest- in every cotton-producing state from Virginia to Texas. This beetle migrated from Mexico to the United States in the 1890s, feasting on our countrys abundant cotton crop. The boll weevil has cost Americas cotton producers more than $15 billion in yield losses and control efforts. Through the cooperative boll weevil eradication program, APHIS and its partners have spent nearly $1 billion fighting this damaging pest, helping cotton growers rid their crop of this pest and increasing their production. This eradication program has been based on timed sprays during growing season and ensuring cotton is completely removed approaching winter so it ...
Boll Weevils Baseball Camps are held at Blossoms Field on the University of Monticello campus in Monticello, Arkansas. Boll Weevils Baseball Camps are led by Head Coach John Harvey and Boll Weevil baseball staff.
article{c02feb63-0c75-498d-9b63-6043d6df0ab2, abstract = {Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure ...
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.in.gov/portal/The-insect-enemies-of-the-cotton-boll-weevil-by/hrnHcC9YI00/ typeof=Book http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.in.gov/portal/The-insect-enemies-of-the-cotton-boll-weevil-by/hrnHcC9YI00/,The insect enemies of the cotton boll weevil, by W. Dwight Burgess, assisted by R.A. Cushman and C.E. Hood,/a,,/span, - ,span property=potentialAction typeOf=OrganizeAction,,span property=agent typeof=LibrarySystem http://library.link/vocab/LibrarySystem resource=http://link.library.in.gov/,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.in.gov/,Indiana State Library,/a,,/span,,/span,,/span,,/span,,/div ...
Advertisement for Toxaphene featuring a photograph of a boll weevil. Originally trademarked by the Hercules Powder Company, Toxaphene was a synthetic organic insecticide toxicant primarily used in agricultural…. ...
Our scientists perform extensive and careful designing of the desired sequence for codon optimization, randomization, and mutation, insertion of reporter gene sequences or tags to facilitate subsequent detection /purification of the desired ligand, cDNA or the antibody. For cDNA phage display libraries, Rx Biosciences uses a novel protocol, which avoids restriction enzyme mediated cDNA truncation, as commonly used by other vendors for cDNA cloning. Hence our protocol enhances the chance of display of full length proteins at the surface of the phages. Rx Biosciences also constructs normalized, subtracted and full-length cDNA libraries in different phage display vectors. We pioneer in recombinant antibody library construction, screening and overexpression for large scale isolation and purification of monoclonal antibodies.. To receive a custom quote, please contact us. We look forward to hearing from you!. Receive a Quote ...
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins. ...
Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. In two of the projects, a three-helix protein domain based on staphylococcal protein A has been used as scaffold to generate so called Affibody molecules capable of binding to key proteins related to two diseases common among elderly people.. In the first project, Affibody molecules were selected using phage display technology for binding to Ab-peptides, believed to play a crucial role in Alzheimers disease, in that they can oligomerize and contribute to the formation of neural plaques in the brain. The selected Affibody molecules were found to efficiently capture Ab from spiked human plasma when coupled to an affinity resin. The ...
The term Boll weevil as it applies to the area of agriculture can be defined as An insect pest of cotton that is the subject of an Animal and Plant Health Inspection Service eradication program cooperatively funded and managed by cotton producers. ...
Pixdaus.com is an environmental photo-bookmarking and sharing service. All the pictures are uploaded by users and the copyrights belong to the rightholders. You can request a removal using the Copyright flag link below each image if you find an image that shouldnt belong here ...
Most topiary gardens that you see are in the shapes of animals or teapots. Not Pearls garden. His topiary is free flowing and surprising. He also branched out and started creating metal sculptures to add to his garden. As we were walking around the house and garden, Pearl himself came out and talked to us. He told us that he grew up in a sharecropper family where he would get beaten for being creative and making things out of found objects. He now gives lectures at universities and has a positive outlook on life. Bishopville is sort of out of the way - actually it is way out of the way, but Pearls garden is something really worthwhile to see. These pictures are just a small sample of what he has in his ex-cornfield ...
John Rollin Ridge or Yellow Bird (1827 - 1867), a citizen of the Cherokee Nation, is considered the first Native American novelist. Ridge was the son of Treaty Party leader John Ridge and the grandson of Major Ridge, longtime Speaker of the Cherokee Nation.
In condimentum facilis porta. Sed nec diam eu diam mattis viverra. Nulla fringilla, orci acerty euismod semper, magna diamert porttitor mauris, quis sollicitudin sapien justo in libero. Mei iriure dignissim ad, prompta dissentiunt te quo. His ut elitr ceteros ...
SongPop 2 is the worlds best music trivia game featuring more than 100,000 songs from your favorite musicians!. iOS 7.0, Android 2.3 ...
Thursday, October 28, 2010 @7:51:26 PM thanks Tuneager. i cant wait to jam with ya! hopefully my schedule will be a little more open soon.. ...
Nitin Kulkarni, president of Corning Display Technologies Taiwan, confirmed at FPD Taiwan 2006 that Corning intends to begin commercial shipments of 8G glass substrates from its Kakegawa (Shizuoka Prefecture, Japan) plant in the third quarter of 2006.
If your weevil problem is limited to the outside of your home, perhaps among the plants in your garden, you could consider this product as one of the best repellents to eliminate boll weevil. One of the weaknesses weevils have is that they cant fly. To get from one plant to another, and to invade your home or garden in the first place, they need to crawl. So if you can isolate the plants you want to protect, chances are good that you will be able to keep them free of the damage that weevils can cause.
Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides[4] Check out refs about bacteria that bioprecipitate heavy metal ...
Apple on Thursday revealed the next step in Retina display technology by packing what it calls the worlds highest resolution display into its popular all-in-one desktop.
... while a C-buffer requires polygons to be displayed from the nearest to the furthest. Because the C-buffer technique does not ... Portal rendering divides a scene into cells/sectors (rooms) and portals (doors), and computes which sectors are visible by ... In 3D computer graphics, hidden-surface determination (also known as shown-surface determination, hidden-surface removal (HSR ... is the process of identifying what surfaces and parts of surfaces can be seen from a particular viewing angle. A hidden-surface ...
Surface texturing is one of techniques used to reduce optical losses to maximize light absorbed. Currently, surface texturing ... Solar panels using mono-Si cells display a distinctive pattern of small white diamonds. ... Perovskite solar cells[edit]. Main article: Perovskite solar cell. Perovskite solar cells are solar cells that include a ... Main articles: Organic solar cell and Polymer solar cell. Organic solar cells and polymer solar cells are built from thin films ...
... which causes an irregularity in the brain surface. The cerebral cortex, which in normal patients is six cell layers thick, is ... The resolution displays the multiple folds within the cortical area, which is continuous with the neuropathology of an infected ... The important aspect to realize is PMG affects each patient differently and treatment options and mitigation techniques will ... specifically cell division, cell migration or neurite growth. Some mutations that affect the role of microtubules and are ...
On microscopic examination, the tumor usually displays sheets of atypical cells with some resemblance to the cells of the ... Minimally invasive surgical techniques remain controversial due to the absence of long-term data, with a particular concern for ... Grossly, ACCs are often large, with a tan-yellow cut surface, and areas of hemorrhage and necrosis. ... The widely used angiotensin-II-responsive steroid-producing cell line H295R was originally isolated from a tumor diagnosed as ...
The various leveled surface of lotus leaves is made out of convex cells (looks like bumps) and a much smaller layer of waxy ... CNT nanoforests can be made using chemical vapor deposition techniques. CNT's can be applied on a surface to modify its water ... Sun et al., have found that CNTs aligned vertically with a medium spacing display the best hydrophobic properties. Small and ... Ratio of surface area of rough surface to the surface area of a flat projection of the same surface For more complex systems ...
Ephrins: Ephrins are cell surface molecules that activate Eph receptors on the surface of other cells. This interaction can be ... In addition, severed commissural axons in chicks, display the capability of translating and expressing Eph-A2 receptor during ... Contemporary diffusion-weighted MRI techniques may also uncover the macroscopical process of axonal development. The connectome ... and activate complexes of cell-surface receptors called Plexins and Neuropilins. Cell adhesion molecules (CAMs): Integral ...
... they press against the cell walls of the phytobiont's root cells. Often the fungal and plant cell walls become almost ... With the advent of more precise genetic techniques, these traits of the mantle are often used in tandem with molecular analyses ... Much as the Hartig net and mantle, composite hyphal organs can display a wide range of structural difference. Some rhizomorphs ... However, this is largely absent in the cortical type, suggesting different strategies of increasing surface contact among ...
These 3D surface objects are also often arranged as "sets". Vaa3D can display multiple sets of any of these surface objects, ... The Virtual Finger technique maps the identified 2D user input via 2D display devices, such as a computer screen, back to the ... and fast cell counting in specific brain areas. Vaa3D also provides methods to annotate these cells and identify their names. ... Irregular surface objects: Each 3D surface has irregular shape and is modeled using complicated surface mesh. ...
In vitro assessments of cells show expression of the Chlamydia genes on cell surfaces through imagining techniques and show ... Potential vaccines using gas vesicle as an antigen display can be given via the mucosal route as an alternative administration ... High concentrations of solute will cause water to be drawn out of the cell by osmosis, causing cell lysis. The ability to ... Gas vesicles occur primarily in aquatic organisms as they are used to modulate the cell's buoyancy and modify the cell's ...
... is more versatile than traditional printing techniques. The surface does not have to be printed under pressure ... This makes it useful for some of the techniques of printing solar cells, electronics etc. ... Graphic screenprinting is widely used today to create mass or large batch produced graphics, such as posters or display stands ... Subsequently, aluminum paste is dispensed over the whole surface of the back for passivation and surface reflection. One of the ...
Surface-related effects can also have a large influence on luminescence color and efficiency. Surface ligands on nanocrystals ... UCNP-based systems can couple both light-based techniques and current-based techniques. This optical stimulation of ... the UCNPs target tumor cells and the plasmonic nanoparticles generate heat to kill cancer cells. [Field] nanoparticles generate ... Flexible, transparent displays have also been fabricated using UCNPs. New security inks which incorporate lanthanide doped ...
Monoclonal antibodies, due to their size, are utilized against cell surface targets. Among the overexpressed targets are ... Display techniques. *Bacterial display. *mRNA display. *Phage display. *Ribosome display. *Yeast display ... CD117 (KIT): for gastrointestinal stromal tumors (GIST) and mast cell tumors. *CD10 (CALLA): for renal cell carcinoma and acute ... Programmed cell death Apoptosis. Pyknosis. Karyorrhexis. Karyolysis. Accumulations. pigment Hemosiderin. Lipochrome/Lipofuscin ...
... scanning was used to reveal the importance of charged residues for the process of transport of the proteins to the cell surface ... This technique can also be used to determine whether the side chain of a specific residue plays a significant role in ... Cyclotides display a wide range of pharmaceutically important bioactivities, but their natural function is in plant defense as ... This technique is rapid, because many side chains are analyzed simultaneously and the need for protein purification and ...
In a more efficient alternative method pursued by, one employs as a simulation cell the hyper spherical surface S 3 {\ ... It is commonly used to choose cubic cells with periodic boundary conditions in conjunction with Ewald summation techniques. ... 2 we display the dipole potential V C D ( χ ) {\displaystyle V_{CD}(\chi )} in (30). With that, the one-dimensional Schrödinger ... Such surfaces can be viewed as geodesics of the surfaces foliating the space like region. Quantum motions on open H 1 4 {\ ...
The epithelial cell rests of Malassez (ERM) presented in the roots surface, principal location of the LPC, play a role in LPC ... It is rare but have been reported in literature that cysts can display mandibular or maxillary bone expansion, bone perforation ... treatment of LPC consists of surgical removal of the lesion by conservative enucleation with guided bone regeneration technique ... In most cases, no inflammatory cells cells are present, but in some rare instances, inflammation in the fibrous capsule can be ...
... from various tumor types can express specific cell-surface molecules (e.g. FasL or CD95) that induce T-cell ... The lipid and protein content of microvesicles has been analyzed using various biochemical techniques. Microvesicles display a ... For example, those released from antigen-presenting cells (APCs), such as B cells and dendritic cells, are enriched in proteins ... Once released from their cell of origin, microvesicles interact specifically with cells they recognize by binding to cell-type ...
Stiff cantilevers provide stability very close to the surface and, as a result, this technique was the first AFM technique to ... In cellular biology, AFM can be used to attempt to distinguish cancer cells and normal cells based on a hardness of cells, and ... The surface topography is commonly displayed as a pseudocolor plot. Although the initial publication about the atomic force ... The force which pulles together the two surfaces is F. =. 2. π. R. γ. L. (. 1. +. cos. ⁡. θ. +. h. r. e. f. f. ). {\ ...
E 65, 21905-10 (2002) A.G. Petrov, A new generation of surface-driven liquid crystal displays, Materials for Information ... Cell.& Molec.Biol.Lett. 2, suppl. 1, 231-253 (1997) Flexoelectricity and photoflexoelectricity in model and biomembranes, A.G. ... Curvature electric effects in artificial and natural membranes studied using patch clamp techniques, A. G. Petrov, R. L. Ramsey ... Electrical Engineers, UK, A.G. Petrov, H. Schmiedel, U. Kühnau, Polar surface interactions vs. long range interactions in the ...
The body's cells constantly display short fragments of their proteins on the cell's surface, and, if a T cell recognises a ... The powerful techniques developed by life sciences are becoming the basis of engineering approaches towards nanomaterials, ... If the cell has the appropriate receptor on its surface, these viruses enter the cell sometimes by direct fusion with the cell ... The causes of death include cell lysis, alterations to the cell's surface membrane and apoptosis.[122] Often cell death is ...
... or surface-enhanced Raman scattering (SERS) is a surface-sensitive technique that enhances ... Effect of surface morphology on SERS detection and application of single algal cell analysis". Biosensors and Bioelectronics. ... Platinum and palladium nanostructures also display plasmon resonance within visible and NIR frequencies. Resonance Raman ... "Surface selection rules for surface-enhanced Raman spectroscopy: calculations and application to the surface-enhanced Raman ...
The gap junctions also connect deeper layers of cells to the surface layer (osteocytes when surrounded by bone). This was ... Enzymatic digestion is one of the most advanced techniques for isolating bone cell populations and obtaining osteoblasts. Peck ... Osteoblast (Wright Giemsa stain, 100x) Light micrograph of decalcified cancellous bone displaying osteoblasts actively ... Individual cells cannot make bone. A group of organized osteoblasts together with the bone made by a unit of cells is usually ...
The sequestration of the metal occurred mainly by sorption to the cell-surface with very little intracellular uptake. Gazem MA ... Selected cultures displayed a good sorption capacity of 32 - 41 mg Pb2+ and 3.5 - 6.5 mg Cu2+ g-1 dry weight of mycelia Joshi ... recovery of gold from electronic waste using mycofiltration techniques. Fungi are amongst the primary saprotrophic organisms in ... In fact, cell walls of some species of dead fungi can be used as a filter that can adsorb heavy metals and radionuclides ...
... cell surfaces that is determined by the net electrical charge of molecules exposed at the surface of cell membranes of the cell ... which displays a wide variety of bizarre red blood cell morphologies: small and highly ovaloid cells in llamas and camels ( ... advantage of the hemoglobin color change to directly measure the arterial blood oxygen saturation using colorimetric techniques ... Red blood cells, also known as RBCs, red cells,[1] red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ...
Black spots can also be found on the surface of infected tubers, which are a sign of the disease. These are made up of conidia ... The discoloration on the periderm of the potato is formed from the loss of pigmentation caused by extreme dryness of the cell ... Some soils displayed suppressiveness in different levels. The results from experimental trials revealed a significant negative ... Another way that silver scurf can be diagnosed is through molecular techniques, such as PCR and sequencing to identify the ...
... on the infected red blood cell surface and the modified display of malaria surface proteins reduces parasite adhesiveness ( ... The molecular basis of sickle cell anemia was finally elucidated in 1959, when Ingram perfected the techniques of tryptic ... In addition, binding of parasitized sickle cells to endothelial cells is significantly decreased because of an altered display ... G6PD is present in all human cells but is particularly important to red blood cells. Since mature red blood cells lack nuclei ...
The data spreadsheet displays survey data in a spreadsheet format. Data can be edited. PseudoShow displays data from a series ... Petros Eikon has moved from standard steepest descent inversion techniques to a Trust Region technique. The design of a survey ... Grid cells need not be square but may be rectangular to correspond to different spatial densities of stations and lines. Data ... Simulation of geophysical models for various EM systems such as surface and borehole TDEM, airborne and ground FDEM, IP/ ...
Glycoprotein molecules on the surface of ejaculated sperm cells are recognized by all human female immune systems, and ... Regarding the values that we have obtained, along with the spermiogram results, different techniques will be displayed. For ... Sperm cells come in two types, "female" and "male". Sperm cells that give rise to female (XX) offspring after fertilization ... Mammalian sperm cells become even more active when they approach an egg cell in a process called sperm activation. Sperm ...
"A novel method for preparing proton exchange membrane fuel cell electrodes by the ultrasonic-spray technique". Journal of Power ... CNT thin films are used as alternative materials to create transparent conducting films (TCO layers) for touch panel displays ... The primary factors influencing the initial droplet size produced are frequency of vibration, surface tension, and viscosity of ... 2009). "Ultrasonic spray deposition for production of organic solar cells". Solar Energy Materials & Solar Cells. 93 (4): 447- ...
... on the infected red blood cell surface and the modified display of malaria surface proteins reduces parasite adhesiveness ( ... The molecular basis of sickle cell anemia was finally elucidated in 1959, when Ingram perfected the techniques of tryptic ... Sickle-cell[edit]. Main article: Sickle-cell anemia. See also: Sickle-cell trait and Evolutionary_baggage § Sickle-Cell and ... In addition, binding of parasitized sickle cells to endothelial cells is significantly decreased because of an altered display ...
Alivisatos went on to use his techniques to create an entirely new generation of hybrid solar cells that combined ... Given that a nanocrystal is virtually all surface and no interior, its properties can vary considerably as the crystal grows in ... display technologies (his quantum dot emissive film is found in the Kindle Fire HDX tablet); and renewable energy (solar ... No techniques had been reported for making two-dimensional or rod-shaped semiconductor nanocrystals that would also be of ...
Less subject to surface artifacts, such as eye and facial movements. *Capable of at-home training due to smaller, more portable ... Photoelectric cells in a spectrophotometer device worn on the forehead measure the amount of each wavelength of light reflected ... Since then, both techniques have been applied to numerous disorders of frontal and prefrontal lobe function. Sherrill, R. (2004 ... The ratio of red/infrared light refraction is displayed as a visual signal on a computer monitor and may also be translated ...
The brain detects insulin in the blood, which indicates that nutrients are being absorbed by cells and a person is getting full ... Some mammals are omnivores and display varying degrees of carnivory and herbivory, generally leaning in favor of one more than ... Among other practices, fasting, dieting, and vegetarianism are all techniques employed by individuals and encouraged by ... Since small mammals have a high ratio of heat-losing surface area to heat-generating volume, they tend to have high energy ...
Their life cycle is thought to begin with a virion attaching to specific cell-surface receptors such as C-type lectins, DC-SIGN ... The first person infected with the disease was the village school's headmaster Mabalo Lokela, who began displaying symptoms on ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ... dendritic cells and other cells including liver cells, fibroblasts, and adrenal gland cells.[93] Viral replication triggers ...
Polymorphonuclear cells also infiltrate the epithelium, and chronic inflammatory cells infiltrate the lamina propria. Atrophic ... Antifungal medication can also be applied to the fitting surface of the denture before it is put back in the mouth. Other ... Oral rinse technique can distinguish between commensal candidal carriage and candidiasis. If candidal leukoplakia is suspected ... Most often, affected individuals display one clear type or another, but sometimes there can be more than one clinical variant ...
The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ... turns bright brownish red with oval white spots all over in a high contrast display.[97] Displays are often reinforced by ... The interior surfaces of the arms are covered with circular, adhesive suckers. The suckers allow the octopus to anchor itself ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ...
Potassium is the major cation (positive ion) inside animal cells,[223] while sodium is the major cation outside animal cells.[ ... When the repulsive forces within the liquid metal surface exceeds the forces of the surface tension, it vigorously explodes.[ ... thallium also displays the oxidation state +3,[10]:28 which no known alkali metal displays[10]:28 (although ununennium, the ... Angelici, R. J. (1999). Synthesis and Technique in Inorganic Chemistry. Mill Valley, CA: University Science Books. ISBN 978-0- ...
Information Display. Society for Information Display. 2006.. *^ Krepelka, J. (1992). "Maximally flat antireflection coatings" ( ... An antireflective or anti-reflection (AR) coating is a type of optical coating applied to the surface of lenses and other ... Other techniques use varying thicknesses of the coatings. By using two or more layers, each of a material chosen to give the ... and antireflective coatings on solar cells.[1] ... A circular polarizer laminated to a surface can be used to ...
The focal length of a lens is determined by its refractive index n and the radii of curvature R1 and R2 of its surfaces. The ... In this technique the objective is dipped into a drop of high refractive index immersion oil on the sample under study.[38]:14 ... due to the unique chatoyance each individual stone displays. A refractometer is the instrument used to measure refractive index ... A differential interference contrast microscopy image of yeast cells. Unstained biological structures appear mostly transparent ...
The surface of conventional activated carbon is reactive, capable of oxidation by atmospheric oxygen and oxygen plasma[26][27][ ... Other regeneration techniques[edit]. Current concerns with the high energy/cost nature of thermal regeneration of activated ... Individual particles are intensely convoluted and display various kinds of porosity; there may be many areas where flat ... to higher temperatures and either combusted to do work or in the case of hydrogen gas extracted for use in a hydrogen fuel cell ...
Electrolysis cells can be either open cell or closed cell. In open cell systems, the electrolysis products, which are gaseous, ... Fleischmann and Pons and co-workers met with Jones and co-workers on occasion in Utah to share research and techniques. During ... The lawyer later apologized; Fleischmann defended the threat as a legitimate reaction to alleged bias displayed by cold-fusion ... Although details have not surfaced, it appears that the University of Utah forced the 23 March 1989 Fleischmann and Pons ...
Odontocetes, such as the sperm whale, possess teeth with cementum cells overlying dentine cells. Unlike human teeth, which are ... The whale faeces are liquid and instead of sinking, they stay at the surface where phytoplankton feed off it.[83][84][85] ... They use a suction technique, aided by a pair of grooves on the underside of their head, not unlike the throat pleats on the ... Humpback whales, among others, are also known to display this behaviour.[68] ...
SiC can react with the surface oxide layer in order to provide diboride surfaces with higher energy: adding 5-30 vol% SiC has ... These techniques rely on metal halide and boron halide precursors (such as TiCl4 and BCl3) in the gaseous phase and use H2 as a ... Both effects reduce the overall bonding strength in the unit cell and therefore the enthalpy of formation and melting point. ... ZrB2/60%SiC composites have been used as novel conducting ceramic heaters which display high oxidation resistance and melting ...
When light passes from air or a vacuum into a transparent crystal, some of it is reflected at the surface and some refracted. ... It is represented by a lattice of points which repeats a basic pattern, called a unit cell, in three dimensions. The lattice ... More recently, driven by advances in experimental technique (such as neutron diffraction) and available computational power, ... display perhaps more of an overlap with materials science than any other discipline. ...
This allows IBM Domino applications to be better surfaced to browser clients, though the UX and business logic must be ... Properties dialog boxes also automatically recognize the type of text selected and display appropriate selections (for instance ... "Techniques to Not Send a Return Receipt When Viewing Mail".. *^ "KillNotes".. *^ Official history of Lotus Notes IBM ... The IBM Domino 9 Social Edition included the IBM Notes Browser Plugin, which would surface Notes applications through a ...
When the bladder is fully distended the superficial cells become squamous (flat) and the stratification of the cuboidal cells ... The technique can help children to urinate discreetly inside cars and in other places without being seen by others.[30] ... "raised-leg display",[89][90][91][91][92] "shadow-urination",[93] or "pseudo-urination".[94] They typically mark their territory ... Domestic dogs mark their territories by urinating on vertical surfaces (usually at nose level), sometimes marking over the ...
That allows the endospore to show up as red, while the rest of the cell stains blue. Another staining technique for endospores ... Bacillus subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of ... While the rest of a bacterial cell may stain, the endospore is left colourless. To combat this, a special stain technique ... A stained preparation of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red ...
If we consider a cell of the lattice formed, we can see that it is a rhombus with the four sides equal to d = p/sin α; (we have ... In the liquid crystal display industry, moiré is often referred to by the Japanese word mura,[citation needed] which roughly ... This technique is attractive because the scale of the moiré pattern is much larger than the deflection that causes it, making ... This can for example be due to surface reconstruction of the crystal, or when a thin layer of a second crystal is on the ...
Other techniques used by blind people to assist them in daily activities include: *Adaptations of coins and banknotes so that ... For example, the Canadian currency tactile feature is a system of raised dots in one corner, based on Braille cells but not ... Access technology such as screen readers, screen magnifiers and refreshable Braille displays enable the blind to use mainstream ... braille is a form of communication in which people use their fingers to feel elevated bumps on a surface and can understand ...
Aish Technologies makes console (display) systems for the Royal Navy off B3068 in Alderney. ... below the surface.[48] The coins were excavated by archaeologists from the Portable Antiquities Scheme.[49] ... developing the technique in the early 1980s. Edwards Sports Products of Bridport, owned by Broxap of Staffordshire, make ... Nearby on Lydiard Fields in Lydiard Tregoze is Johnson Matthey Fuel Cells, which in 2002 was the world's first production site ...
Surface hypoplasias record stressors occurring from about one to seven years, or up to 13 years if the third molar is included ... That as the body attempts to compensate for low iron levels by increasing red blood cell production in the young, sieve-like ... No permission was ever granted from surviving family for study and display. Recently, federal laws such as NAGPRA (Native ... but combines the standard forensic techniques of describing stature, sex and age with investigations of demography and ...
... surface layer homology domains, and surface layer-cell wall interactions". J Struct Biol. 124 (2-3): 276-302. doi:10.1006/jsbi. ... Theron, J.; Cloete, T.E. (2000). "Molecular techniques for determining microbial diversity and community structure in natural ... acidophilic archaea display promise for the extraction of metals from ores, including gold, cobalt and copper.[207] ... Further information: Cell wall § Archaeal cell walls. Most archaea (but not Thermoplasma and Ferroplasma) possess a cell wall.[ ...
... and other stem cells) revealed many gene promoters carrying both H3K4Me3 and H3K27Me3, in other words these promoters display ... Using an electron paramagnetic resonance spin-labeling technique, British researchers measured the distances between the spools ... Tropberger P, Schneider R (Jun 2013). "Scratching the (lateral) surface of chromatin regulation by histone modifications". ... SLBP levels are controlled by cell-cycle proteins, causing SLBP to accumulate as cells enter S phase and degrade as cells leave ...
Käss, W. Tracing Technique in Geohydrology. Rotterdam: Balkema.. *^ The Story Behind Dyeing the River Green. Greenchicagoriver. ... In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence ... This application is common in yeast display. Fluorescein can also be conjugated to nucleoside triphosphates and incorporated ... used as a rather conservative flow tracer in hydrological tracer tests to help in understanding of water flow of both surface ...
Stem cells may be of interest in transitional tissue welding.[citation needed] ... The lead removal technique will vary depending on the surgeon's estimation of the probability that simple traction will suffice ... Some devices have the availability to display intracardiac electrograms of the onset of the event as well as the event itself. ... connected to electrode leads which passed through the skin of the patient to terminate in electrodes attached to the surface of ...
Hydrogen fuel cells background information Fuel cells operate similar to a battery in that electricity is harnessed from ... Further, it was reported that "the technique is not difficult" and that "there is so much coffee around that several hundred ... Once the oil is liberated from the shoreline, the oil-biodiesel mixture is manually removed from the water surface with ... Biodiesel has displayed its capacity to significantly dissolve crude oil, depending on the source of the fatty acids. In a ...
A cell membrane isolates the inside of the cell from its environment. Neurons do not communicate via direct cytoplasm to ... New techniques are however rapidly emerging. Search on "Single neuron imaging" and see related topics: Biological neuron model ... Electrocorticography intracranial EEG, the practice of using electrodes placed directly on the exposed surface of the brain to ... Analysis of Functional NeuroImages an open-source environment for processing and displaying functional MRI data ...
British developmental biologist noted for her contributions to stem cell research and transgenic technology and techniques. She ... These questions seem different on the surface, but all of them share one quality that makes them impossible to answer: all of ... activities consist of the coming together of large groups of people to perform repeated and prolonged submissive displays to ... of cells in the cell cycle.[253]. *Mark Oliphant (1901-2000): Australian physicist and humanitarian. He played a fundamental ...
"Cell. 138 (4): 774-86. doi:10.1016/j.cell.2009.07.038. PMC 3210731. PMID 19703402.. ... proteins that function as dimers may form a heterodimer composed of one protein from each alternate gene and may display ... the fitness where all genotypes are arranged in 2D space and the fitness of each genotype is represented by height on a surface ... "iLOCi: a SNP interaction prioritization technique for detecting epistasis in genome-wide association studies". BMC Genomics ...
As bit cell size decreases, more data can be put onto a single drive platter. In 2013, a production desktop 3 TB HDD (with four ... Therefore, the surface of the magnet is half north pole and half south pole, with the radial dividing line in the middle, ... "MJM Data Recovery Ltd: Hard Disk Bad Sector Mapping Techniques". Datarecovery.mjm.co.uk. Archived from the original on February ... These techniques store extra bits, determined by mathematical formulas, for each block of data; the extra bits allow many ...
The streak of a mineral is independent of trace elements[70] or any weathering surface.[74] A common example of this property ... Minerals that often display parting include the pyroxenes, hematite, magnetite, and corundum.[77][79] ... Cell Biology and Mineral Deposition. by Kenneth Simkiss; Karl M. Wilbur On Biomineralization. by Heinz A. Lowenstam; Stephen ... is observable with various techniques, such as thin-section petrography.[87] ...
... attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. ... Receptor proteins in nerve and muscle cells have ... Unable to display preview. Download preview PDF.. References. * ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. ... Application of Fast Reaction Techniques to Kinetic Measurements of Receptor Function on Cell Surfaces. In: Kito S., Segawa T., ...
We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of ... Cell Surface Display Techniques * Enzyme Activation / drug effects * Enzyme Inhibitors / pharmacology* * Humans ... We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of ...
It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. Peptides that ... Cell Surface Display Techniques * Hydrogen Bonding * Models, Molecular* * Molecular Dynamics Simulation * Mutation / genetics ... bind to NRP-1 and are internalized into the cell. We used peptide phage display binding assays and molecular dynamics (MD) ... It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. Peptides that ...
1. phage display 2. ribosome display 3. mRNA-peptide fusion 4. plasmid display 5. cell-surface display 6. genetics 7. n-hybrid ... There are a number of commercially available kits for error-prone PCR (link to Stratagene). Similar techniques also use ... Screening techniques. List of high-throughput assays for protein function ... There are several common techniques used to construct libraries of functional variants ...
GM7373 cells were cultured in flasks on a shaker that rhythmically tilted a thin layer of medium across the apical cell surface ... Endothelial Cell Volume and Stiffness Measurements.. Volume of living GM373 cells was determined with AFM techniques as ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Increasing Potassium Swells Endothelial Cells.. AFM imaging of living endothelial cells reveals that cell height and cell ...
After engagement of the cell surface with the particle tip, the cantilever will be bent as the cell is indented. A laser beam ( ... refer to the total volume of the cells displayed. The graph shows the mean values of cell heights at the 3 different potassium ... Indentation technique using AFM. (A) Cantilever with particle tip. (B) Principle of the stiffness measurement using an AFM. (C ... Time course of cortical cell stiffness measurements (means of 4 cells) in response to trypsin. The increase of cortical cell ...
Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. Sci. Signal. 7, rs4 ... Introducing these constructs into inert cell lines, such as HeLa epithelial cells and Cos-7 fibroblast-like cells, these cells ... exposing the C2 domain on the cell surface so that it can bind to PS on the surface of the target apoptotic cell. Simultaneous ... Wang et al. showed that Rac activation in a single cell directed the migration of border cells, a collection of 6 to 10 cells ...
25028719 - Rapidly rendering cells phagocytic through a cell surface display technique and concurr.... 20945119 - Artesunate ... of cells in the chondrocyte-like cell aggregates were positive for Scx, whereas only 3.4% of cells in the fibroblast-like cell ... Cell proliferation Ki-67 is a cell-proliferation marker [5]. In normal ACLs, 27.2% ± 4.1% of ligament cells were Ki-67 positive ... Cell density and cell arrangements The cell density in histologically normal ACLs from young donors (,45 years old) with normal ...
Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ... Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, ...
... technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells ... Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...
Cell Surface Display Techniques ... Using phage display techniques, a high-affinity monoclonal ... and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo. ...
... of cell-based selection techniques has allowed selection against surface proteins in their native configuration on the cell ... All cell surface proteins cycle intracellularly to some extent, and many surface receptors are actively internalized in ... Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. ... Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking ...
Since the introduction of the yeast display platform, this method has increasingly gained popularity for the discovery and ... Overall, this chapter underlines the importance of the versatile yeast display technique for the optimization of the novel Fcab ... Sádio F., Stadlmayr G., Stadlbauer K., Rüker F., Wozniak-Knopp G. (2019) Yeast Surface Display and Cell Sorting of Antigen- ... Chao G, Lau WL, Hackel BJ et al (2006) Isolating and engineering human antibodies using yeast surface display. Nat Protoc 1(2): ...
Cell surface display allows peptides or proteins to be expressed on the cell exterior as fusions to natural host anchoring ... Electrostatically Actuated Microvalves Fabricated with Soft-Lithographic Techniques for Integrated Microfluidics  Tice, Joshua ... Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. Tight regulation of cadherin adhesion plays critical ... Intracellular redox environment, the relative amount of oxidized and reduced chemical species within a cell, is important in ...
To display proteins on the cell surface of S. cerevisiae, molecular information of the native cell wall protein, α-agglutinin, ... This anchoring signal was combined with the signal of the secreted enzymes by genetic engineering techniques. Actually, we ... a cell surface glycoprotein involved in cell-cell interactions during mating. Mol. Cell. Biol. 9 1989 3155 3165 ... The display of CMCase and β-glucosidase proteins on the cell surface was confirmed by immunofluorescence microscopy. The cells ...
We discuss the strengths and pitfalls of different techniques for anti-neuronal antibody detection: 1) Immunohistochemistry and ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface ... The detection of neuronal surface autoantibodies in patients serum or CSF therefore has serious consequences for the patients ...
Synthetic nanopillars resembling the wing surface of certain insects display antibacterial activities. Here, Jenkins et al. ... The nanopillars induce deformation and penetration of the Gram-positive and Gram-negative bacterial cell envelope, but do not ... They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of ... This has inspired the design of antibacterial implant surfaces with insect-wing mimetic nanopillars made of synthetic materials ...
... and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. The ... The present invention relates to tumor tropic stem cells, ... terms may refer to the presence or display of a cell-surface ... Local intravascular administration by means of a catheter is a common technique in medical practice. For example, catheters as ... or any method which selectively binds the specific cell surface molecules. Alternatively, the method may use the cell surface ...
ROLs would be stabilized by the cell wall components and that ROL activity could be easily improved by display techniques. The ... of cells was obtained and used as a whole-cell catalyst. The amount of ROL displayed on the yeast cell surface was estimated to ... We used a displayed ROL and mutants of this enzyme immobilized on the yeast cell surface in organic solvents as whole-cell ... Yeast cell-surface display-applications of molecular display. Appl. Microbiol. Biotechnol.64:28-40. ...
Fn14, a cell surface receptor overexpressed in many cancers including pancreatic and triple-negative breast cancers, was ... and mirror-image phage display (d-peptide) techniques, respectively. The d-peptide ligand successfully mediated targeted drug ... Although excessive cell damage results in passive necrosis, cells can be triggered to engage molecular programs that result in ... Although a number of cell types are able to produce TNF, the ability of CD8+ T cells to produce TNF following viral infection ...
... while a C-buffer requires polygons to be displayed from the nearest to the furthest. Because the C-buffer technique does not ... Portal rendering divides a scene into cells/sectors (rooms) and portals (doors), and computes which sectors are visible by ... In 3D computer graphics, hidden-surface determination (also known as shown-surface determination, hidden-surface removal (HSR ... is the process of identifying what surfaces and parts of surfaces can be seen from a particular viewing angle. A hidden-surface ...
... video signal and wherein the passive display is capable of displaying a preset range of gray levels and presenting a displayed ... a light source to provide illumination of the passive display; a video signal input, wherein in response to a video signal the ... and wherein the intensity of light provided by the light source illuminating the display is controlled based on the ... passive display modulates light from the light source to provide an image, ...
... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... The BACE-1 inhibitors prepared displayed IC50 values in the range of 4-45 nM, where the most potent compounds featured small P1 ... Wilkinsons catalyst [RhCl(PPh3)3] was heterogenized on common silica by the use of a grafting/anchoring technique. The ... In a preceding study we have described the development of a new hydroxyethylene (HE) core motif displaying P1 aryloxymethyl and ...
... spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces ... Here, we show that biosynthetic enzymes can display unexpected promiscuity, with human glycosyltransferase pp-a-GanT2 able to ... TEM revealed that after several catalytic runs many of the Pd nanoparticles (NPs) had migrated to the external surface of the ... demonstrating that this technique may prove useful for the sequencing of complex oligosaccharides. ...
These techniques revealed not only the ganglion cell impulses, but also the slow changes in ganglion cell membrane potential ... The arrangement of ganglion cells on the retinal surface is not spatially random. Each cell type is distributed in an ordered ... In the right column gain has been increased to better display the lower amplitude rod signals. Stimulus brightness is given in ... Y cells are called brisk transient cells, and X cells are called brisk sustained cells (Cleland and Levick, 1974a). Y cells ...
... or both on their outer membranes or cell surfaces.. Our efforts focus on the discovery of novel carbohydrate-binding proteins ... and trafficking of tumor cells during metastasis. Not to be forgotten all microbes and many viruses display unique glycan ... This is accomplished using glycan profiling and biophysical techniques, evaluation of antimicrobial or antiviral activities, ... The initial step of HIV infection involves stepwise binding of the surface envelope glycoproteins, gp120/gp41, to cellular ...
Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity ... Visit for more related articles at Journal of Analytical & Bioanalytical Techniques View PDF Download PDF ... due to sheer number of cells to sort), or how well the peptide is displayed on the cell surface. In this test case, both ... for cell sorting using yeast surface display [28-31], as well as for sorting tumor epithelial cells for downstream screening of ...
Although NHE8 displays a broad tissue distribution, it was unknown if NHE8 is expressed in the ocular surface. Here, we ... detected NHE8 expression by PCR and Western blot, and located NHE8 protein by immunohistochemistry technique. As shown in Fig. ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... only NHE1 was detected in the ocular surface epithelial cells, and its function involves cell volume regulation (47). Since ...
The use of phage display technique for the isolation of androgen receptor interacting peptides with (F/W)XXL(F/W) and FXXLY new ... Identification and Characterization of a Novel Androgen Receptor Coregulator ARA267-α in Prostate Cancer Cells. Wang, X., Yeh, ...
In Vitro Liquid Extraction Surface Analysis Mass Spectrometry (ivLESA-MS) for Direct Metabolic Analysis of Adherent Cells in ... Che Chemistry Techniques. Translation:HumansCells * Sanders MA, Brock JE, Harrison BT, Wieczorek TJ, Hong X, Guidi AJ, Dillon ... Association of Cell-Free DNA Tumor Fraction and Somatic Copy Number Alterations With Survival in Metastatic Triple-Negative ... Fibroblast-tumor cell signaling limits HER2 kinase therapy response via activation of MTOR and antiapoptotic pathways. Proc ...
  • Receptor proteins in nerve and muscle cells have attracted much attention recently because of their central role in the transmission of signals between nerve and muscle cells. (springer.com)
  • SELEX has traditionally been performed using purified proteins, and cell surface receptors may be challenging to purify in their properly folded and modified conformations. (mdpi.com)
  • However, improvements in recombinant fusion protein technology have increased the availability of receptor extracellular domains as purified protein targets, and the development of cell-based selection techniques has allowed selection against surface proteins in their native configuration on the cell surface. (mdpi.com)
  • All cell surface proteins cycle intracellularly to some extent, and many surface receptors are actively internalized in response to ligand binding. (mdpi.com)
  • The display of CMCase and β-glucosidase proteins on the cell surface was confirmed by immunofluorescence microscopy. (asm.org)
  • To display proteins on the cell surface of S. cerevisiae , molecular information of the native cell wall protein, α-agglutinin, was utilized. (asm.org)
  • α-Agglutinin is a cell surface adhesion molecule involved in mating ( 11 ) and has a glycosylphosphatidylinositol anchor attachment signal, which is engaged in anchoring of cell wall proteins ( 10 , 27 ). (asm.org)
  • They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of oxidative stress proteins. (nature.com)
  • Proteins are immobilized by using an outer shell cell wall protein, the C-terminal half of α-agglutinin ( 10 , 26 ). (asm.org)
  • Not to be forgotten all microbes and many viruses display unique glycan structures, carbohydrate-binding proteins, or both on their outer membranes or cell surfaces. (nih.gov)
  • sodium/hydrogen exchangers (NHEs) are a group of membrane proteins that exchange extracellular Na + for intracellular H + , and they display broad tissue distribution. (physiology.org)
  • GAGs interact with proteins displayed on the surface of cells to control signalling events. (europa.eu)
  • However, study of these interactions is very challenging as the GAGs sit on the surface of proteins and not in deep binding pockets, and are attracted through a series of weak interactions. (europa.eu)
  • These techniques have allowed the Fellow to look at footprinting of specific GAGs onto the surface of proteins such as factor H module 7 (fHapp.7) which is thought to play an important role in the development of age-related macular degeneration (one of the leading causes of blindness in the Western world). (europa.eu)
  • Using molecular biological techniques, Sutter's team have altered the structure of the MVA genome in such a way that genetic information coding for proteins characteristic of other viral pathogens can be spliced into it. (uni-muenchen.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing NGF domain in the selected taxonomic class. (embl-heidelberg.de)
  • Directed evolution of proteins is a technique used to modify protein functions through "Darwinian selection. (hindawi.com)
  • Liposome-based IVC is characterized by in vitro protein synthesis from a single copy of a gene in a cell-sized unilamellar liposome and quantitative functional evaluation of the synthesized proteins. (hindawi.com)
  • Target proteins fused with a membrane protein are displayed on the cell membrane surface, screened for the desired function by exposure to a colorimetric detection reagent, and selectively sorted using a fluorescence-activated cell sorter (FACS) [ 4 , 5 ]. (hindawi.com)
  • In this technique, target proteins are fused with phage coat proteins ( g8p or g3p ) and displayed on a phage surface. (hindawi.com)
  • The first area of focus is to examine the polarized trafficking of two integral membrane proteins in epithelial cells, the transferrin receptor (TR) and the cystic fibrosis transmembrane conductance regulator (CFTR). (uab.edu)
  • One thing to consider: in the absence of aptamers that bind to the cell surface, we might as well try it with aptamers that bind proteins in vitro as done in 12000850. (openwetware.org)
  • Previous studies have revealed that the C-terminal region of the S-layer protein from Lactobacillus is responsible for the cell wall anchoring, which provide an approach for targeting heterologous proteins to the cell wall of lactic acid bacteria (LAB). (biomedcentral.com)
  • Moreover, both of the fused proteins could simultaneously bind to the surface of a single cell. (biomedcentral.com)
  • The LcsB region can be used as a functional scaffold to target the heterologous proteins to the cell surfaces of lactic acid bacteria in vitro and in vivo , and has also the potential for biotechnological application. (biomedcentral.com)
  • S-layer proteins normally contain two functional regions: the self-assembly domain and the cell wall-targeting domain. (biomedcentral.com)
  • In the S-layer proteins of lactobacilli, no S-layer homology (SLH) motif at the C-terminus, which was responsible for the non-covalent attachment of the protein subunits to the cell wall through a pyruvylated polysaccharide receptor in the cell wall [ 6 ], has been identified. (biomedcentral.com)
  • The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)
  • These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)
  • The proteins then fold under oxidizing conditions and are displayed on the cell surface or packaged into phage particles to screen for binding affinity 17,18 . (jove.com)
  • To improve the efficiency of engineering antibodies that are well folded in the cytoplasm, we previously reported the success of MAD-TRAP (membrane-anchored display for Tat-based recognition of associating proteins), a method for screening an scFv antibody library using Escherichia coli inner-membrane display 19 . (jove.com)
  • Influenza type A is the most common virus to infect humans, and in order to bind to host cells it uses two proteins on its surface: haemagluttinin and neuraminidase. (www.nhs.uk)
  • The virus is further divided into varieties depending on which types of these two proteins it has on its surface. (www.nhs.uk)
  • Once an antibody is selected, the genes for that antibody are transferred into an expression system-bacteria, yeast, or mammalian cell lines specially designed for the expression of foreign proteins. (jhsph.edu)
  • The AIDA system was then used for the expression of two Salmonella surface proteins, SefA and H:gm, with potential for use as vaccine epitopes. (diva-portal.org)
  • Since a live bacterial vaccine based on surface display of antigens has many advantages over traditional vaccines, we have studied the surface display of the SE antigenic proteins, H: gm and SefA in Escherichia coli by the beta-autotransporter system, AIDA. (diva-portal.org)
  • Surface display is a powerful technique that utilizes natural microbial functional components to express proteins or peptides on the cell exterior. (unt.edu)
  • Cross-reactive anti-heat-shock-protein antibodies and T cells can damage vascular tissues overexpressing warmth shock proteins, contributing to the development of atherosclerosis (Mandal et al. (cylch.org)
  • One of the most important mechanisms by which cells receive and decode signals from the outside world is by adding or removing phosphate from specific tyrosine amino acids in cellular proteins, which alters the functions of the modified proteins. (cancer.gov)
  • Like the PTKs, PTPs comprise a large gene family that encodes both cell surface (receptor-like) and intracellular (non-transmembrane) proteins. (cancer.gov)
  • Antibodies are the first proteins which were successfully displayed on the surface of phage by fusing the coding sequence of scFv or Fab to the coat protein. (creative-biolabs.com)
  • I think I mentioned to you already that I began to work with a colleague, Mark Willingham, studying how hormones and proteins bound to the cell surface, how they behaved on the cell surface, how they entered cells, and the pathway by which they entered cells. (nih.gov)
  • And since we were working with receptors, and receptor mediated endocytosis, we said that this vesicle that we see inside the cell that is moving around and that contains hormones and other proteins, should be called a receptosome. (nih.gov)
  • We measured the rates of internalization of different proteins through this vesicular pathway, how many different molecules could reside in the same vesicle, how many vesicles were formed per minute, diffusion rates of receptor-hormone complexes on the cell surface and the likelihood they would get into a vesicle and be internalized. (nih.gov)
  • Their novel strategy introduces specific, dynamic and reversible supramolecular functionality on the bacterial cell surface by adopting a bacterial display system that has been used before exclusively to identify high affinity peptides for various proteins. (nanowerk.com)
  • To overcome these drawbacks, new imaging technologies have been described recently that are based on the detection of resonance energy transfer (RET) between fluorescent or luminescent proteins (FPs or LPs) ( Box 1 ) [ 5 ] and on protein fragment complementation approaches [ 6 , 7 ], both of which facilitate real-time characterization and visualization of PPIs in live cells. (pubmedcentralcanada.ca)
  • Tissue cells are surrounded by the complex of fibrous proteins and sugars, called the extracellular matrix (ECM). (baylor.edu)
  • Dr. Zachary Crook, a postdoctoral research fellow in the Olson Lab, helped design a lab-based method for screening thousands of tiny proteins in human cells. (fredhutch.org)
  • To find that one engineered protein, the team used a new method developed by Fred Hutch postdoctoral research fellow Dr. Zachary Crook and brain cancer researcher Dr. Jim Olson that is capable of screening thousands of tiny proteins in human cells in the lab. (fredhutch.org)
  • The technique Crook and Olson developed uses human cells to display thousands of different tiny proteins on their surfaces and a special molecular signaling system that alerts the researchers if one of those tiny proteins attaches to the drug target they're after, like TEAD. (fredhutch.org)
  • RVSE-induced apoptosis by upregulating the p53 level in a dose- and time-dependent manner 17-AAG cell signaling and by activating apoptosis-associated proteins, 17-AAG cell signaling such as Bax/Bcl-2, cleaved caspase-3 and -9 and cleaved PARP, in MCF-7 cells. (bioskinrevive.com)
  • The differentiated cells can be analyzed by real-time PCR and flow cytometry to confirm enrichment of EC-specific genes and proteins. (pubmedcentralcanada.ca)
  • Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. (mdpi.com)
  • With cell-based selection, a specific protein target is not always chosen, but selection is performed against a target cell type with the goal of letting the aptamer choose the target. (mdpi.com)
  • Since the introduction of the yeast display platform, this method has increasingly gained popularity for the discovery and affinity maturation of antibodies and other protein scaffolds intended for antigen recognition. (springer.com)
  • 4. The isolated stem cell of claim 2, wherein said isolated stem cell exhibits a glial fibrillary acidic protein (GFAP) astrocytic precursor marker. (freepatentsonline.com)
  • 16. The method of claim 14, wherein said stem cell exhibits a glial fibrillary acidic protein (GFAP) astrocytic precursor marker. (freepatentsonline.com)
  • In this study, we evaluated applications of genetically immobilized Rhizopus oryzae lipase displayed on the cell surface of Saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with α-agglutinin. (asm.org)
  • Thus, yeast cells displaying the lipase can be used in organic solvents, and the lipase activity may be increased by a combination of protein engineering and display techniques. (asm.org)
  • Protein-carbohydrate interactions play critical roles in countless biological processes and recognition events as diverse as fertilization, leukocyte homing during the course of inflammation, and trafficking of tumor cells during metastasis. (nih.gov)
  • Immunohistochemistry staining located NHE8 protein at the plasma membrane of the epithelial cells in the conjunctiva, the cornea, and the lacrimal gland both in human and mouse. (physiology.org)
  • This will then allow the determination of the distance between the ends of the GAG chain by fluorescence (yielding vital structural information about the GAG), and also "footprinting" of the GAG upon the surface of a protein (yielding vital information about the GAG binding site on the protein). (europa.eu)
  • The modified virus is then able to synthesize the foreign protein and displays it on the surface of infected cells. (uni-muenchen.de)
  • cell surface display via Ice Nucleation Protein, and phage display via M13's major coat protein. (igem.org)
  • A more radical proposal involves use M13 phage as the scaffold, and attaching enzymes by phage-display techniques to the pVIII coat protein. (igem.org)
  • Herein, we introduce a new liposome-based IVC system consisting of a liposome, the protein synthesis using recombinant elements (PURE) system and a fluorescence-activated cell sorter (FACS) used as a microcompartment, in vitro protein synthesis system, and high-throughput screen, respectively. (hindawi.com)
  • Subsequently, the protein variants are synthesized from the mutated genes using living hosts (cells) or an in vitro transcription-translation system (IVTT), and they are screened for the desired function. (hindawi.com)
  • For example, cell-surface display is an in vivo screening technique that uses a fusion protein to localize protein molecules to a cell membrane surface. (hindawi.com)
  • Phage display is another in vivo display technique that uses a phage for gene storage and protein display. (hindawi.com)
  • It is a fusion protein N - Lpp aa's 1-9 - OmpA aa's 46-159 - surface protein - C. E. coli K12 genome and BioBrick primers for the full OmpA gene (start through stop) have been ordered. (openwetware.org)
  • In this study, we developed a new surface display system in lactic acid bacteria with the C-terminal region of S-layer protein SlpB of Lactobacillus crispatus K2-4-3 isolated from chicken intestine. (biomedcentral.com)
  • Surface layers (S-layers), which are composed of protein or glycoprotein subunits, are the outmost cell surface structure of many Bacteria and Archaea. (biomedcentral.com)
  • We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
  • Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
  • They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)
  • It is a selection technique in which a library of variants of a peptide or protein is expressed on the outside of a phage virion, while the genetic material encoding each variant resides on the inside. (neb.com)
  • This creates a physical linkage between each variant protein sequence and the DNA encoding it, which allows rapid partitioning based on binding affinity to a given target molecule (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called panning. (neb.com)
  • Genes encoding antigen-binding variable domains of antibodies are fused to phage genes that encode coat protein, allowing for the phage coat to express, or "display", the antibody fusion protein. (jhsph.edu)
  • Vasotide, they found, keeps a protein called vascular endothelial growth factor, VEGF, from binding to two different kinds of receptors on the surface of endothelial cells. (unm.edu)
  • However, while much basic research is directed towards mechanisms of surface expression using autotransporters there are few reports regarding production of surface expressed protein. (diva-portal.org)
  • Thus the aim of this work was the optimisation of the yield and productivity of surface expressed protein. (diva-portal.org)
  • Protein Z, an IgG-binding domain of Staphylococcal protein A, was used as a model protein for the investigation of which cultivation parameters influenced surface expression. (diva-portal.org)
  • The removal of proteolysis and further optimisation of the yield of surface-expressed protein are important goals of further work. (diva-portal.org)
  • Microbial cell-surface display has proven to be extremely important for numerous applications ranging from combinatorial library screening and protein engineering to bioremediation and biofuels production. (unt.edu)
  • In vitro stimulation of peripheral blood mononuclear cells from a patient bearing SSX-4 -expressing tumor with a pool of long peptides spanning the protein sequence was used for assessment of SSX-4 -specific CD4 + T cells recognizing distinct antigenic sequences restricted by HLA class II alleles. (aacrjournals.org)
  • The inherently hydrophobic surfaces of MPs complicates protein expression, purification, and characterization. (rsc.org)
  • ShuA variants were displayed on the surface of an M13 bacteriophage as fusions to the P8 coat protein. (rsc.org)
  • To counter the aggregated protein’s toxic effects, cells induce several responses, such as the ER overloading response (ERO), unfolded protein response (UPR), and autophagy.Several therapeutic methods have been proposed to remove the toxic aggregation,such as small molecule inhibition, autophagy up-regulation, RNA interference,and genomic correction. (ufl.edu)
  • Our future studies will focus on the detailed mechanisms of the protein-protein interaction and how cells respond to these two therapies. (ufl.edu)
  • M. T. Reetz,* J. Sanchis Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process ChemBioChem DOI: 10.1002/cbic.200800371 Surface Chemistry Perfect support: Microfabrication techniques to create arrays of identical liquid-crystalline areas for membrane support and protein display are demonstrated. (docme.ru)
  • In the March issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc3_10.dtl ), Shao-En Ong from the Broad Institute of MIT and Harvard ( www.broadinstitute.org/proteomics/team.html ) presents Unbiased Identification of Protein/Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics . (cshlpress.com)
  • Billions of yeast cells, each bearing a unique antibody, are then mixed with the protein of interest. (harvard.edu)
  • The IPI's first major effort will be to develop open-source libraries of well-validated antibodies targeting every human protein found outside cells. (harvard.edu)
  • The ZapA cell division protein (blue) localizes to the FtsZ ring. (nih.gov)
  • Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. (creative-biolabs.com)
  • This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. (creative-biolabs.com)
  • Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype. (creative-biolabs.com)
  • Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific target binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naïve targets. (creative-biolabs.com)
  • This was genetically fused to a modified outer membrane protein eCPX, which enabled it to be displayed on the outer membrane and bind CB[8]. (nanowerk.com)
  • In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. (pubmedcentralcanada.ca)
  • They describe the new technique and the engineered protein in a paper published Thursday in the journal Nature Communications. (fredhutch.org)
  • Illustration of a designed peptide (blue) sitting on the surface of the TEAD protein (pink). (fredhutch.org)
  • Human cells are much better at making and tying the protein knots, it turns out. (fredhutch.org)
  • Therefore, relatively few aptamers have been generated that bind cell surface receptors. (mdpi.com)
  • Several studies have demonstrated that aptamers that bind cell surface receptors may have functions other than just blocking receptor-ligand interactions. (mdpi.com)
  • Therefore, aptamers that bind cell surface receptors have been exploited for the delivery of a variety of cargoes into cells. (mdpi.com)
  • Yeast display is particularly well suited for the selection of antigen-binding Fc fragments (Fcabs) as it allows rapid combinatorial library construction via gap repair-driven homologous recombination and an efficient display of a glycosylated Fc able to interact with Fcγ receptors. (springer.com)
  • The stem cells with tumor tropic potential are selected based on the stem cells exhibiting CXCR4 receptors or an affinity for the chemokine SDF-1. (freepatentsonline.com)
  • This information is in the form of chemical messages sensed by receptors on the ganglion cell membrane. (utah.edu)
  • The initial step of HIV infection involves stepwise binding of the surface envelope glycoproteins, gp120/gp41, to cellular receptors, CD4 and CCR5 or CXCR4. (nih.gov)
  • Dendritic cells are equipped with receptors for antigen (HIV), with cell adhesion molecules, and receptors for immunoregulatory and costimulatory signals. (natap.org)
  • Using phage display analysis, Dr. Collawn's lab has identified peptides that bind to cell surface receptors on dendritic and B cells. (uab.edu)
  • They are screening for specific receptors that undergo RME in order to develop methodologies for enhancing antigen uptake and processing by antigen-presenting cells in vitro. (uab.edu)
  • At MDACC, Drs. Pasqualini and Arap used a technique called phage display to generate a vast library of specific peptides that home to both known and novel receptors. (nanowerk.com)
  • and (2) peptides were identified that specifically bind these and previously known receptors and are rapidly internalized by the cells of interest. (nanowerk.com)
  • Alvos has identified rapidly internalizing cell surface receptors for over 30 diseased and normal tissue types and peptides that efficiently target them. (nanowerk.com)
  • Normally, when VEGF binds to one of its receptors the binding causes endothelial cells to multiply, allowing blood vessels to form. (unm.edu)
  • The glycan receptors for AB5 toxin B subunit pentamers are displayed either on glycolipids (for Ctx/LT and Stx) or on glycoproteins (for Ptx and SubAB). (functionalglycomics.org)
  • Megakaryocytic differentiation is accompanied by changes in cell morphology, notably an increase in size and the appearance of demarcation membranes, and by the sequential expression of a number of genes coding for specific cell surface markers, cytokines, and cytokine receptors. (bloodjournal.org)
  • Swiss researchers have designed a groundbreaking technique that uses artificial receptors to enhance the body's immune response to tumors. (medicalnewstoday.com)
  • In contrast, a low dose of trypsin, known to activate sodium influx through epithelial sodium channels, stiffens the submembranous cell cortex. (pnas.org)
  • Similarly, as shown in renal epithelium with scanning ion conductance microscopy ( , 12 ), aldosterone stimulates endothelial cell volume, growth, and stiffness by activating epithelial sodium channels (ENaCs) ( , 13 , , 14 ). (pnas.org)
  • Sodium/hydrogen exchanger (NHE) 8 is expressed at the apical membrane of the epithelial cells and plays important roles in neutral sodium absorption in the gastrointestinal tract and the kidney. (physiology.org)
  • We also detected the expression of downregulated-in-adenoma (DRA, a Cl − /HCO 3 − transporter) in the ocular surface epithelial cells. (physiology.org)
  • Altered ocular surface function and reduced epithelial DRA expression in NHE8−/− mice suggest that the role of NHE8 in ocular surface tissue involve in tear production and ocular epithelial protection. (physiology.org)
  • Among them, five NHEs are detected at the plasma membrane in the epithelial cells in the intestine and the kidney. (physiology.org)
  • NHE1 is detected in almost all tissues and is located at the basolateral membrane in the epithelial cells ( 39 , 42 ). (physiology.org)
  • NHE2 and NHE3 are mainly expressed at the apical membrane in the intestinal and the renal epithelial cells ( 9 , 14 , 39 ). (physiology.org)
  • NHE4 is also detected in the gastric epithelial cells and renal epithelial cells ( 39 ). (physiology.org)
  • NHE8, the latest characterized NHE isoform, is expressed at the apical membrane in the intestinal and renal epithelial cells ( 25 , 49 ). (physiology.org)
  • This mouse model mimics the majority of human BRCA1 breast cancers and exhibits significant alterations in mammary gland morphology, increased mammary epithelial cell proliferation, and upregulation of the progesterone receptor. (army.mil)
  • The investigators have demonstrated that treatment of these BRCA1/p53 conditional knockout mice with the anti-progesterone mifepristone significantly delayed the onset of mammary tumor development, reduced mammary gland morphological dysfunction, and retarded mammary epithelial cell and hyperplastic foci progression to tumors. (army.mil)
  • For example, differentiated airway epithelial cells can revert into stable and functional stem cells in vivo after the ablation of airway [24] . (wikiversity.org)
  • Chen W-T, Greve JM, Gottlieb DI, Singer SJ (1985) Immunocytochemical localization of 140 kD cell adhesion molecules in cultured chicken fibroblasts, and in chicken smooth muscle and intestinal epithelial tissues. (springer.com)
  • Goblet cells are epithelial cells that secrete mucins onto the ocular surface to maintain the wet environment for protection with tear. (postech.ac.kr)
  • After EBs are formed and exposed to endothelial differentiation medium containing VEGF, a heterogeneous cell population, including mesenchymal, hematopoietic, and epithelial cells, emerges. (pubmedcentralcanada.ca)
  • Peptides that expose the consensus sequence R/K/XXR/K at the C-terminus (C-end rule or CendR peptides) bind to NRP-1 and are internalized into the cell. (nih.gov)
  • We used peptide phage display binding assays and molecular dynamics (MD) simulations to study the potential role of the central residues of CendR peptides in binding and activation of the NRP-1 receptor. (nih.gov)
  • Here, we have developed a methodology for bacterial cell sorting using the semi-automated autoMACS® Pro Separator for the first time, and have produced a complete method for sorting of bacteria displaying 15-mer peptides on their cell surface using this device, including downstream bioinformatic analysis of candidates for binding to a target of interest. (omicsonline.org)
  • Peptides presented by human leukocyte antigen (HLA) molecules on the cell surface play a crucial role in adaptive immunology, mediating the communication between T cells and antigen presenting cells. (mcponline.org)
  • Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action and in cellular immunotherapy and transplantation. (mcponline.org)
  • Knowledge of these peptides is of pivotal importance in fundamental studies of T cell action, the design of T-cell-mediated therapies such as tumor immunotherapy ( 1 ), and the treatment of hematological malignancies through a combination of hematopoietic stem cell transplantation and donor lymphocyte infusion ( 2 ). (mcponline.org)
  • Although these techniques are very useful as initial screening methods, they do not allow for the identification of the exact molecular target of the autoantibodies. (frontiersin.org)
  • Although excessive cell damage results in passive necrosis, cells can be triggered to engage molecular programs that result in cell death. (bireme.br)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the w:embryo . (wikiversity.org)
  • Using molecular techniques the researchers investigated how the antibody was protecting mice. (www.nhs.uk)
  • It is possible to perform all of these techniques in a standard molecular biology lab or the process can be outsourced to a variety of facilities specializing in the generation of rAbs. (jhsph.edu)
  • This is a fascinating and unconventional route for antigen presentation to T cells, which does not require complex and rate-limiting molecular interactions inside the dendritic cell. (medicalnewstoday.com)
  • To circumvent these problems, researchers including British biochemist Greg Winter invented robust "molecular display" technologies. (harvard.edu)
  • By doing so, Jonkheijm, who heads research into Bioinspired Molecular Engineering, and his team, were able to show that living cells can also be used as a component in supramolecular smart materials. (nanowerk.com)
  • In the Taube lab, we are investigating the molecular pathways and cellular properties which enable primary tumor cells to metastasize. (baylor.edu)
  • A quantitative thermodynamic model proposed that the bactericidal activity of nanopatterned surfaces is directly related to the balance between adhesion energy and deformation energy. (nature.com)
  • The Adhesion of Particles Undergoing an Elastic-Plastic Impact with a Surface", J. Phys. (patentgenius.com)
  • and (3) after a period of time measuring the distance from a fixed point in the measuring chamber in a direction towards the outlet thereof over which cells remain on the said surface in the measuring chamber after the passage of fluid therethrough,the distance measurement giving a measure of the adhesion of the cells to the substratum. (patentgenius.com)
  • 4. Apparatus as claimed in claim 3, wherein the measuring chamber is of rectangular cross-section and either one or both of the opposed pair of parallel internal faces of the chamber are formed from or coated with a substratum whose adhesion tothe cells under test is to be measured. (patentgenius.com)
  • 6. Apparatus as claimed in claim 4, wherein a wall of the chamber in which cells remain after the test is calibrated so that measurement of cell adhesion can be made by direct observation. (patentgenius.com)
  • Such surfaces would allow for the simultaneous investigations of adhesion site maturation and composition and how changes in these properties can be implemented to engineer cell behavior via cell-surface interactions. (asme.org)
  • For example, nanostructures can be used to mimic patterns of surfaces found in nature to endow the synthetic surfaces with properties such as inherent colour effects, tack-free adhesion to surfaces, water-proofing and anti-reflectivity. (nanotech-now.com)
  • The cell-ECM interaction is mediated by integrin molecules and plays crucial roles in cell organization such as cell adhesion, migration, and differentiation. (baylor.edu)
  • We now demonstrate effects of extracellular potassium on stiffness of individual cultured bovine aortic endothelial cells by using the tip of an atomic force microscope as a mechanical nanosensor. (pnas.org)
  • As potassium has a beneficial effect on cardiovascular function ( , 17 - , 20 ), we have now examined the relation between extracellular potassium and cell stiffness. (pnas.org)
  • AFM imaging of living vascular endothelial cells, exposed to increasing concentrations of extracellular potassium. (pnas.org)
  • Relationship between extracellular potassium concentration and cortical cell stiffness. (pnas.org)
  • Hartline pushed experimental technology of the 1930's to its limits in obtaining extracellular recordings of individual ganglion cells. (utah.edu)
  • Fibronectin containing extracellular material connects surfaces of developing granulocytes with surfaces of stromal cells. (springer.com)
  • Campbell A, Wicha MS, Long M (1985) Extracellular matrix promotes the growth and differentiation of murine hematopoietic cells in vitro. (springer.com)
  • Granit also made the first ganglion cell recordings from a mammalian retina, the cat retina. (utah.edu)
  • TR is expressed in nearly all mammalian cells and its itinerary is well characterized by us and others. (uab.edu)
  • Such modifications can be used to enhance viral binding and entry to a wide variety of both dividing and nondividing mammalian cells, as well as to produce antibodies against the displayed antigen. (cshlpress.com)
  • Its distribution is highly restricted, being mostly limited to a subset of sensory hair cells in the mammalian inner ear and elsewhere to a subset of immune cells. (baylor.edu)
  • A variety of approaches are used to identify targets or determine mechanisms of action, including mutagenesis and genome sequencing, and cell based and functional assays. (nih.gov)
  • In earlier assays, the researchers had confirmed the immunogenicity of their candidate vaccine in cell culture, and demonstrated that it elicits the production of protective levels of MERS-CoV-neutralizing antibodies. (uni-muenchen.de)
  • While HIV-specific CD4 clones are depleted directly and indirectly by HIV infection, sensitive assays can detect cells capable of producing interferon gamma in response to HIV antigens despite advancing immunodeficiency. (natap.org)
  • Once the rAbs are displayed, tools such as paramagnetic beads, fluorescence-activated cell sorting (FACS), and/or Enzyme-Linked Immunosorbent Assays (ELISAs) can be used to isolate individual antibodies that bind to a specific antigen target. (jhsph.edu)
  • Flow cytometry showed that surface EnvG bound various conformationally- and trimer-specific antibodies (Abs), and in-vitro growth assays on CD4+CCR5+ cells demonstrated EnvG functionality. (jove.com)
  • Tissue sections were analyzed for cell density, cell organization, ECM components, ECM-degrading enzymes and markers of differentiation, proliferation, and stem cells. (biomedsearch.com)
  • In contrast, ACL degeneration is associated with cell recruitment or proliferation, including progenitor cells or myofibroblasts. (biomedsearch.com)
  • This finding has led to the development and testing of second-generation small-molecule compounds that could be used clinically to inhibit cell proliferation and target Rad51 interactions. (army.mil)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves, and then differentiate into the cell types needing replacement in the damaged tissue [26] Macrophages can self-renew by local proliferation of mature differentiated cells. (wikiversity.org)
  • 1 In the second compartment, terminal maturation is accompanied by a decrease in proliferation, an increase in cell ploidy, and cytoplasmic maturation. (bloodjournal.org)
  • Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. (asm.org)
  • Initial attachment of cells is followed by proliferation and biofilm formation, with sessile cells in biofilms displaying properties that are dramatically different from their planktonic (free-living) counterparts. (asm.org)
  • Deregulation of oncogenes leads to explosive cell induction and proliferation of invasiveness, which promotes the acquisition of a malignant phenotype [2]. (bioskinrevive.com)
  • Effects of RVSE on MCF-7 Cell Proliferation and Cell Cycle Arrest To examine its anticancer activity, we assessed the effects of 0C400 (bioskinrevive.com)
  • The unlimited differentiation and proliferation capacity of embryonic stem cells represents a great resource for regenerative medicine. (pubmedcentralcanada.ca)
  • Mast cells and NGF appear to be involved in neuroimmune interactions and tissue inflammation, with NGF acting as a general 'alert' molecule capable of recruiting and priming tissue defence processes following insult as well as systemic defensive mechanisms. (embl-heidelberg.de)
  • they tend to spread themselves as flat as possible, have minimal cell-cell interactions and display different expression and morphogenesis. (labx.com)
  • It has been selected as a paradigm because the other AB5 toxin sub-families referred to above have been known for many years, and a substantial body of information on toxin-receptor interactions had been built up using conventional biochemical techniques [1] [4] . (functionalglycomics.org)
  • Both techniques neglect individual interactions. (hindawi.com)
  • There is no evidence of fibronectin being associated with blood-stromal cell interactions involving either erythropoiesis or thrombopoiesis. (springer.com)
  • This mini-review provides a guideline for both diagnostic and research laboratories that work on the detection of known surface autoantibodies and/or the identification of novel surface antigens. (frontiersin.org)
  • In addition, we discuss the use of immunoprecipitation and mass spectrometry analysis for the detection of novel neuronal surface antigens, which is a crucial step in further disease classification and the development of novel CBAs. (frontiersin.org)
  • In addition, antibodies directed at classical PNS antigens are mostly recognizing linear epitopes, whereas surface antigens contain mostly conformational epitopes. (frontiersin.org)
  • For the detection of neuronal surface antigens also immunocytochemistry (ICC) of primary hippocampal neurons is used. (frontiersin.org)
  • These cells play an important role in directing the immune response when foreign antigens are encountered. (natap.org)
  • HIV antigens are presented by DCs to CD4 and CD8 cells, leading to the expansion of HIV-specific CD4 and CD8 clones. (natap.org)
  • They identified populations of T cells responding (i.e. producing interferon gamma) to either HIV antigens or (as a control) CMV antigens, and sorted these cells by flow cytometry. (natap.org)
  • The evidence of humoral immunity to SSX-2 and SSX-4 , and SSX-4 -specific CD4 + T cells among circulating lymphocytes in patients with antigen expressing EOC suggest that these antigens are attractive targets for specific immunotherapy in EOC. (aacrjournals.org)
  • The development of immunotherapeutic strategies in EOC requires the identification of tumor antigens expressed in these tumors and the design of vaccination strategies that may result in the generation of tumor antigen-specific T cells that could afford durable protection. (aacrjournals.org)
  • As a consequence of advances in the approaches for analyzing humoral ( 3 ) and cellular ( 4 ) immune reactivity to cancer in the context of the autologous host, an increasing number of tumor antigens recognized by autologous CD8 + T cells ( 5 ) and/or antibodies ( 6 ) have been identified. (aacrjournals.org)
  • Thus, this technique can be established for medium to high throughput screening of antigens and antibodies, such as newly-isolated antibodies. (jove.com)
  • The few in vitro studies investigating the impact of malaria on HIV-1 have demonstrated that exposure to soluble malarial antigens can increase HIV-1 infection and reactivation in immune cells. (jove.com)
  • They capture antigens from foreign bodies and hand them over to killer T cells, which then neutralize the threat. (medicalnewstoday.com)
  • To create dendritic cell vaccines, dendritic cells are removed from the patient and "force-fed" tumor antigens before being released back into the patient. (medicalnewstoday.com)
  • One major drawback is that the tumor antigens used to "feed" the dendritic cells are from lab-grown tumors, not the patient's own. (medicalnewstoday.com)
  • Two images of EVIR-engineered dendritic cells (green) capturing tumor antigens in exosomes (gold/red). (medicalnewstoday.com)
  • Using imaging techniques, the team demonstrated that EVIRs enhanced transfer of tumor antigens from the exosome to the outer membrane of the dendritic cell. (medicalnewstoday.com)
  • We call this phenomenon cross-dressing, which alludes to the fact that the dendritic cells acquire immunogenic antigens from the tumor and directly display them on their own surface. (medicalnewstoday.com)
  • Individual candidates were evaluated for relative binding to fluorescently-labeled PA target or streptavidin negative control using Fluorescence-Activated Cell Sorting (FACS). (omicsonline.org)
  • rAbs can be used in all applications in which traditional mAbs are used such as western blotting, immunohistochemistry, fluorescence-activated cell sorting (FACS), and immunofluorescence. (prolabmarketing.com)
  • The differentiated endothelial population expresses both Fetal Liver Kinase 1 (Flk-1) and VE-Cadherin on the cell surface which can be further purified using a fluorescence-activated cell sorting (FACS) system and subsequently expanded on 0.1 % gelatin-coated plates. (pubmedcentralcanada.ca)
  • They used a technique called 'antibody phage-display library' to produce antibody-like molecules that were highly responsive to this virus. (www.nhs.uk)
  • As many flu subtypes have similar chemical sequences in molecules on their surface, the researchers then examined how well the antibodies would bind with other viruses in cell cultures. (www.nhs.uk)
  • Using new equipment we developed and a technique called video intensification microscopy, described in a paper in Cell , we began to look at molecules moving about in vesicles within the cell. (nih.gov)
  • Although this study focused specifically on CB[8], the techniques demonstrated by the researchers can also be used to develop and study other strains for different host molecules. (nanowerk.com)
  • Cell-associated molecules are measured with a variety of probes, most often fluorochrome-labeled monoclonal antibodies. (cancer.gov)
  • Ingela is currently an Associate Professor in analytical chemistry at the Department of Chemistry-BMC, Uppsala University, Sweden, and her research focuses on the development and application of ambient surface sampling coupled to mass spectrometry for endogenous small molecules in biological systems. (rsc.org)
  • Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. (mdpi.com)
  • Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. (springer.com)
  • Traxlmayr MW, Faissner M, Stadlmayr G et al (2012) Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast. (springer.com)
  • These approaches commonly use phage display, yeast surface display, or bacterial surface display to screen large libraries of antibodies 11-13 . (jove.com)
  • These techniques rely on rapid cloning of immunoglobulin gene segments to create libraries of antibodies with slightly different amino acid sequences from which antibodies with desired specificities can be selected. (jhsph.edu)
  • Their protocol for Creation of Baculovirus Display Libraries is featured, and is freely available on the journal's website ( cshprotocols.cshlp.org/cgi/content/full/2010/3/pdb.prot5393 ). (cshlpress.com)
  • With over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. (creative-biolabs.com)
  • Antibody libraries are constructed by the genomic information coding for antibody variable domains, which can be derived from B cells of immune or naïve donors. (creative-biolabs.com)
  • We have addressed this issue for two intimately related serine proteases, tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (uPA), by modifying the technique of substrate phage display to create substrate subtraction libraries. (elsevier.com)
  • In the presence of aldosterone, plasma sodium in the high physiological range stiffens endothelial cells and reduces the release of nitric oxide. (pnas.org)
  • An acute increase of potassium in the physiological range swells and softens the endothelial cell and increases the release of nitric oxide. (pnas.org)
  • Endothelial cells are softened by estrogens through activation of a plasma membrane sodium/proton antiporter, yet are insensitive to progesterone and testosterone ( , 11 ). (pnas.org)
  • Superficial and Deep Stiffness Are Detectable in Living Endothelial Cells. (pnas.org)
  • Typical indentation curves of living endothelial cells are not exponential in shape. (pnas.org)
  • Indentation curves obtained in 2 experiments on vascular endothelial cells. (pnas.org)
  • Nitrite concentrations analyzed in the supernatant culture media after exposure of the vascular endothelial cells for 24 h to different potassium concentrations. (pnas.org)
  • OxTrp72-apoA1 recovered from human atheroma or plasma is lipid poor, virtually devoid of cholesterol acceptor activity, and demonstrated both a potent proinflammatory activity on endothelial cells and impaired HDL biogenesis activity in vivo . (acc.org)
  • Endothelial cells line the inside of blood vessels of normal organs and tumors. (unm.edu)
  • Here, we describe a method for differentiating, isolating, and expanding endothelial cells (ECs) from mouse embryonic stem cells (mESCs). (pubmedcentralcanada.ca)
  • Methods for developing differentiated endothelial cells from EBs are well established and the corresponding gene expression patterns are well characterized ( 4 - 6 ). (pubmedcentralcanada.ca)
  • Embryonic stem cells have been directly differentiated toward specific lineages without first making EBs by using conditioned medium for endothelial differentiation of ESC in collagen IV plates ( 7 , 8 ). (pubmedcentralcanada.ca)
  • Embryoid bodies can be directed to give rise to hemangioblasts, which subsequently undergo further differentiation into either hematopoietic or endothelial cells. (pubmedcentralcanada.ca)
  • Only a subset of these cells will differentiate toward endothelial cells (less than 2 % with our current method). (pubmedcentralcanada.ca)
  • Investigations by fast reaction techniques with membrane vesicles, Biochemistry 20: 3467 (1981). (springer.com)
  • In agreement with a previous report ( , 21 ), we assume that the first slope represents the plasma membrane stiffness and includes a contribution from the submembrane cortical cytoskeleton, whereas the second slope, which is approximately three times larger than the first slope, represents the stiffness of the inner bulk of the cell. (pnas.org)
  • The biophysical model suggested that cicada wing nanopillars induce physical stretching of the cell membrane upon contact, leading to bacterial rupture and death, and cell rigidity was reported to be an important determinant of susceptibility 14 . (nature.com)
  • Each bacterium generates several enzymes and displays them on its outer membrane. (igem.org)
  • Could this be done by displaying them on a cell outer membrane? (igem.org)
  • The method harnesses the intrinsic intracellular folding quality control mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway to display an scFv library on the E. coli inner membrane. (jove.com)
  • Following removal of the outer membrane, the scFvs displayed on the inner membrane are panned against a target antigen immobilized on magnetic beads to isolate scFvs that bind to the target antigen. (jove.com)
  • Bacterial inner-membrane display relies on the twin-arginine translocation (Tat) pathway for transporting displayed antibodies, in contrast to other common display methods that use the secretory pathway. (jove.com)
  • SefA was verified located on the cell surface, and H:gm was found in the outer membrane of the host cell, though only in proteolytically truncated forms lacking the His 6 -tag used for detection. (diva-portal.org)
  • For example, TonB-dependent transporters (TBDTs) in the outer membrane of Gram-negative bacteria play an essential role transporting iron and other nutrients into the bacterial cell. (rsc.org)
  • It is over-expressed in the new blood vessels grown by tumor cells and is a target for anti-tumor treatments. (nih.gov)
  • The technology, designed to specifically bind and enter tumor cells and the vasculature supporting tumors, is thought to be the world's largest library of human-derived targeting agents, representing thousands of unique peptide sequences. (nanowerk.com)
  • In this way, killer T cells are primed to destroy tumor cells, which are normally experts at evading the immune system. (medicalnewstoday.com)
  • Through an in vivo approach, the genotype-phenotype link is produced by using a living cell. (hindawi.com)
  • Using a well-defined transgenic animal model, they are testing the effectiveness of different peptide sequences identified by phage display to promote antigen uptake and presentation and are using this methodology to study how this might be used to either enhance or suppress helper T cell responses in vivo. (uab.edu)
  • These studies employ both cell biological and immunological techniques to understand how helper T cell responses are regulated in vitro and in vivo. (uab.edu)
  • Furthermore, to improve upon current in vivo gene delivery methods, COE investigators have engineered specialized expression vectors with cancer-specific promoters, a two-step transcriptional activation system, and post-translational regulatory elements that are capable of expressing therapeutic genes by several hundred-fold in breast cancer cells only. (army.mil)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into w:endodermal , ectodermal and mesodermal cells both in vitro and in vivo. (wikiversity.org)
  • In addition to multiple implications in the regenerative potential of other tissues, mesenchymal stem cells have been shown to promote vessel formation both in vivo and in vitro. (nanohub.org)
  • 3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (labx.com)
  • Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (labx.com)
  • Consequently, administration of anti-CD25 Abs is a commonly used technique to deplete Treg populations in vivo. (nih.gov)
  • Combinando phage display in vivo e microscopia eletrônica de transmissão, observamos a presença de partículas de fago ligadas à vasculatura cerebral em um nível supramolecular: aglomerados de fagos filamentosos expressando o motivo FRW foram visualizados ligados às regiões de contato entre as células endoteliais. (bvsalud.org)
  • Using in vivo phage display, we show that a new peptide motif that emerged from our combinatorial screening of the vasculature binds selectively to blood vessels in the brain in vivo but not to vessels in other organs. (bvsalud.org)
  • Two new approaches allow kinetic measurements of receptor function to be made on cell surfaces with a time resolution of μs to ms. (springer.com)
  • Hess, G.P. and Udgaonkar, J.B., Chemical kinetic measurements of transmembrane processes using rapid reaction techniques: Acetylcholine receptor, Ann. (springer.com)
  • Shusta EV, Holler PD, Kieke MC et al (2000) Directed evolution of a stable scaffold for T-cell receptor engineering. (springer.com)
  • 1. An isolated stem cell, which is isolated by a method, comprising selecting the stem cell based on the stem cell exhibiting a CXCR4 receptor, demonstrating an affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Integral in the regulation and initiation of death receptor-mediated activation of programmed cell death is the aspartate-specific cysteine protease (caspase)-8. (bireme.br)
  • It binds as a dimer to at least 2 cell-surface receptor types expressed in a variety of neuronal and non-neuronal cells. (embl-heidelberg.de)
  • Early genes, such as mpl coding for the TPO receptor 9 and the GPIIb gene 10 are first expressed in CD34 + cells, but are still expressed in the late stages leading to platelet production. (bloodjournal.org)
  • Cells connect to the ECM via a cell surface receptor, integrin. (baylor.edu)
  • The estrogen receptor-positive MCF-7 breasts cancer cell range was produced from the pleural effusion of an individual with metastatic breasts cancer [3]. (bioskinrevive.com)
  • Since Saccharomyces cerevisiae lacks the cellulase complexes that hydrolyze cellulosic materials, which are abundant in the world, two types of hydrolytic enzymes involved in the degradation of cellulosic materials to glucose were genetically co-immobilized on its cell surface for direct utilization of cellulosic materials, one of the final goals of our studies. (asm.org)
  • This result showed that the cell surface-engineered yeast with these enzymes can be endowed with the ability to assimilate cellooligosaccharides. (asm.org)
  • Since enzymatic hydrolysis of cellulose has the potential to surmount many of the drawbacks of acid hydrolysis ( 12 , 13 ), we have attempted to genetically immobilize cellulolytic enzymes in their active form on the cell surface of the yeast S. cerevisiae to construct a novel cellulose-utilizing yeast. (asm.org)
  • This anchoring signal was combined with the signal of the secreted enzymes by genetic engineering techniques. (asm.org)
  • Here, we show that biosynthetic enzymes can display unexpected promiscuity, with human glycosyltransferase pp-a-GanT2 able to utilize both uridine diphosphate N-acetylglucosamine and uridine diphosphate N-acetylgalactosamine, leading to the synthesis of epimeric glycopeptides in vitro. (jic.ac.uk)
  • E. coli displaying multiple enzymes. (igem.org)
  • M13 phage displaying multiple enzymes. (igem.org)
  • Furthermore, hemicellulolytic enzymes displayed on the yeast cell surface hydrolyzed hemicelluloses that were not hydrolyzed by a commercial enzyme, leading to increased sugar utilization for improved ethanol production. (biomedcentral.com)
  • The surface expression of enzymes and vaccine epitopes offer several advantages. (diva-portal.org)
  • Surface expressed enzymes gain similar properties to immobilised enzymes, mainly simplified handling and separation using centrifugation. (diva-portal.org)
  • I was mainly interested in how the enzymes within the cell work. (nih.gov)
  • These results demonstrate that the combination of substrate phage display and substrate subtraction methods can be used to discover specificity differences between very closely related enzymes and that this information can be utilized to create highly selective inhibitors. (elsevier.com)
  • 8. The isolated stem cell of claim 6, wherein said polypeptide is involved in an immune response. (freepatentsonline.com)
  • Once there, it can be recognized by cells of the immune system, which are stimulated to produce antibodies and T cells against it. (uni-muenchen.de)
  • The production of interferon gamma by T cells in response to a particular antigen is proof of a pre-existing mature and specific immune response. (natap.org)
  • Other NGF-responsive cells are now recognized as belonging to the haemopoietic-immune system and to populations in the brain involved in neuroendocrine functions. (embl-heidelberg.de)
  • Asthma is a chronic inflammatory disease of the airways in which innate and adaptive immune cells participate as drivers of the inflammatory response. (uab.edu)
  • Surface expressed vaccine epitopes can have longer half-lives inside the animal that is to be immunized and surface groups on the host cell can act as adjuvants, increasing the immune response and leading to a better immunisation. (diva-portal.org)
  • Our immune system is excellent at destroying and removing damaged, faulty, or old cells, but in the case of cancer, it tends to need a little help. (medicalnewstoday.com)
  • Dendritic cells - also referred to as antigen-presenting cells - are an important part of the immune system. (medicalnewstoday.com)
  • Scientists have discovered that a type of immune cell that normally helps fight infection may actually assist lung tumors. (medicalnewstoday.com)
  • The dendritic cells can then inform the killer T cells and boost the patient's immune response to the cancer. (medicalnewstoday.com)
  • pass on of irregular cells and it is caused by human hormones, immune circumstances, and deregulation of oncogenes [1]. (bioskinrevive.com)
  • Additionally, we want to make one of these aptamers bind to the E. coli cell surface. (openwetware.org)
  • What aptamers exist that bind the E. coli cell surface? (openwetware.org)
  • Display on the cell or phage surface of the cloned antibody sequence allows for rapid, high throughput selection of recombinant antibodies that bind a specific antigen. (jhsph.edu)
  • SubB has recently been shown to bind to N-linked glycans displayed on several glycoproteins on the surface of Vero and HeLa cells, including α2β1integrin [5] . (functionalglycomics.org)
  • Each ShuA variant was analyzed for its ability to display on the bacteriophage surface, and functionally bind to hemoglobin. (rsc.org)
  • The nanopillars induce deformation and penetration of the Gram-positive and Gram-negative bacterial cell envelope, but do not rupture or lyse bacteria. (nature.com)
  • As compared with yeast and phage display, bacterial display is ideal because of the fast doubling time of bacteria, about 20 minutes for Escherichia coli ( E. coli ) [ 7 ] versus about 2 hours for Saccharomyces cerevisiae ( S. cerevisiae ) [ 8 , 9 ], and direct amplification of the bound bacterial cells containing plasmid DNA encoding the displayed peptide responsible for binding, without elution and reinfection. (omicsonline.org)
  • After mixing them with the non-genetically modified lactic acid bacteria cells, the fused GFP-LcsB and Gal-LcsB were functionally associated with the cell surface of various lactic acid bacteria tested. (biomedcentral.com)
  • Autotransporters have been used for surface expression in Gram-negative bacteria. (diva-portal.org)
  • Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. (unt.edu)
  • According to the researchers, one could imagine that these bacteria can carry within the bloodstream microscopic cargo, like drugs in a micro-capsule, and when triggered release this cargo at a particular site, like a cancerous or infected cell. (nanowerk.com)
  • Our analysis shows that a cell component derived from bacteria appears to stimulate egg laying, a serotonin-dependent reproductive behavior, while it also stimulates the movement of worms. (baylor.edu)
  • Flow cytometry is a unique experimental technology that provides rapid, quantitative, multiparametric, single cell analysis and separation. (cancer.gov)
  • Instrumentation includes two customized three-laser (two tunable argon or krypton and rhodamine 6G argon-pumped dye laser) flow cytometers with electronic cell separation capabilities that are operated by staff of the Flow Cytometry Laboratory in support of multiple research projects. (cancer.gov)
  • In addition, staff provide training and consultation in flow cytometry techniques, protocol design, reagent selection, and data analysis and presentation. (cancer.gov)
  • Customized flow cytometry data analysis and data archiving software is developed and maintained, including automated cluster analysis software for statistical classification of quantitative multiparametric data and n-plot graphics display of multiparameter data on either subpopulations or individual cells. (cancer.gov)
  • Biological phenomena, such as cellular differentiation and phagocytosis, are fundamental processes that enable cells to fulfill important physiological roles in multicellular organisms. (sciencemag.org)
  • Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells . (wikiversity.org)
  • This meant that the cells can change their differentiation pathway. (wikiversity.org)
  • In w:Drosophila imaginal discs, cells have to choose from a limited number of standard discrete differentiation states. (wikiversity.org)
  • The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikiversity.org)
  • Stem cells have demonstrated remarkable potential for tissue engineering and regenerative medicine through their ability for self-renewal and controlled differentiation. (nanohub.org)
  • Human cord blood CD34 + progenitor cells were cultured in the presence of cytokines inducing megakaryocytic differentiation. (bloodjournal.org)
  • Embryonic stem cells (ESCs), which are derived from the inner cell mass of blastocysts, are capable of self-renewal and differentiation along different cell lineages, a property known as pluripotence ( 1 ). (pubmedcentralcanada.ca)
  • Peptide candidates produced with this method contained the previously reported PA consensus WXCFTC, further validating this method and the commercially available autoMACS® platform as the first low cost, semi-automated biopanning approach for bacterial display that is widely accessible and more reliable than the MACS/FACS standard protocol. (omicsonline.org)
  • Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have allowed the production of highly specific recombinant antibodies. (frontiersin.org)
  • Recombinant antibodies are translated from recombinant DNA and displayed on the surfaces of cells or phage particles. (jhsph.edu)
  • A collection of recombinant phage that display unique antigen binding domains on their surfaces is known as a phage display library. (jhsph.edu)
  • Sera from a subgroup of the patients were tested for SSX-2 and SSX-4 antibody by ELISA and recombinant antigen expression on yeast surface (RAYS). (aacrjournals.org)
  • Recombinant antibodies (rAbs) are synthetic antibodies that are created using antibody genes made in a laboratory or taken from human cells, completely eliminating animals from the antibody-production process. (prolabmarketing.com)
  • These antibodies are translated from recombinant DNA and displayed on the surfaces of cells or phage particles. (prolabmarketing.com)
  • Then, the cDNA clones encoding variable heavy and variable light chains of these four mabs were isolated, and used for construction of four single-chain Fv (scFv) antibody genes, which were expressed in Escherichia coli cells. (go.jp)
  • Researchers insert the genes for antibodies into one-celled organisms such as yeast, modified so that the antibodies made by the yeast are displayed on their surface. (harvard.edu)
  • Using a gene editing technique, the CRISPR-CAS9 system, we are generating mutations in candidate genes and characterizing genetic or biochemical links to integrin. (baylor.edu)
  • Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. (mdpi.com)
  • Bacterial display sorting is a powerful emerging technology that offers a rapid, high-throughput approach to discovery of robust affinity reagents [ 1 - 3 ]. (omicsonline.org)
  • The team has previously used an advanced high-throughput screening technique called phage display to discover a small peptide that ultimately was chemically modified to become Vasotide. (unm.edu)
  • In addition, the technique can be scaled up for the high-throughput approaches of genomics and proteomics. (harvard.edu)
  • During the first plenary lecture on Monday (L2), Melissa Pope discussed the role of dendritic cells (DCs) in HIV infection and pathogenesis. (natap.org)
  • Recent work has demonstrated that exposure of immature monocyte-derived dendritic cells to the malarial pigment hemozoin increased their ability to transfer HIV-1 to CD4+ T cells 6,7 , but that it decreased HIV-1 infection of macrophages 8 . (jove.com)
  • Dendritic cells are extracted from a patient and EVIRs are inserted into them. (medicalnewstoday.com)
  • When the dendritic cells are returned to the patient's body, they are primed to recognize types of small vesicle called exosomes . (medicalnewstoday.com)
  • EVIRs trap exosomes traveling through the body, giving dendritic cells the exact blueprint of the resident tumor. (medicalnewstoday.com)
  • However, these studies used whole cell extracts of P. falciparum schizont stage parasites and peripheral blood mononuclear cells (PBMC), making it hard to decipher which malarial component(s) was responsible for the observed effects and what the target host cells were 4,5 . (jove.com)
  • Further experiments revealed that the antibodies did not prevent initial binding of the virus to blood cells, but prevented the later step when the membranes of the two cells fuse. (www.nhs.uk)
  • Profilee Satyajit Mayor discusses his explorations of cell membranes, which are helping to update the classical fluid mosaic model of dynamic cellular boundaries. (the-scientist.com)
  • While some advances have been made by other groups where supramolecular elements were introduced on cell membranes by merging artificially made membranes, this is the first time where the cells were made to produce and display supramolecular elements on their surfaces through genetic engineering. (nanowerk.com)
  • These HLA ligands can form the basis for reversed immunology approaches to identify T cell epitopes based not on in silico predictions but on the bona fide eluted HLA ligandome. (mcponline.org)
  • In 1990 John McCafferty demonstrated that variable regions from antibodies could be displayed on the surface of a filamentous phage. (jhsph.edu)
  • NHEs have various physiological functions including intracellular pH homeostasis, cell volume regulation, acid-base regulation, and electroneutral NaCl transport ( 22 , 53 ). (physiology.org)
  • As a result, antibodies isolated using these techniques will not necessarily fold well in the cytoplasm, and intracellular solubility must often be engineered separately if the antibodies will be used in intracellular applications. (jove.com)
  • and 6) separation and analyses of genetically engineered cells for studies of intracellular processes. (cancer.gov)
  • Acute changes in sodium or potassium affect only the first slope that is produced by the cell's shallow outer shell and leave the second slope produced by the inner part of the cell unchanged. (pnas.org)
  • In 1888 Russian physicist Aleksandr Stoletov built the first cell based on the outer photoelectric effect discovered by Heinrich Hertz in 1887. (wikipedia.org)
  • Actually, we reported previously the genetic immobilization of carboxymethylcellulase (CMCase) from Aspergillus aculeatus on the yeast cell surface ( 16 ). (asm.org)
  • In this laboratory study, researchers used genetic and biochemical techniques to select antibodies that could neutralise H5N1 infection both in mice and cell cultures outside living bodies. (www.nhs.uk)
  • Each variable region gene is spliced into a vector (vehicles that transfer foreign genetic material into another cell), and the vector is taken up and integrated into the genome of a microorganism. (jhsph.edu)
  • To address these issues, he and his laboratory members take a combined biochemical, cell biological, and genetic approach. (cancer.gov)
  • Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. (frontiersin.org)
  • Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. (mdpi.com)
  • However, widespread use is limited, in part due to poor performance achieved using manual Magnetic-Activated Cell Sorting (MACS) methods, and an absence of widely-available, low cost, high-performance sorting alternatives. (omicsonline.org)
  • Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. (unt.edu)
  • Professor Ki Hean Kim explained about his future plans, "This study is meaningful, because the new imaging method overcame the limitations of conventional examination methods of conjunctival goblet cells. (postech.ac.kr)
  • TNF inhibits HCV infection despite increased HCV envelope glycoprotein-mediated infection of liver cells. (bireme.br)
  • Ali IU, Hynes RO (1978) Effects of LETS glycoprotein on cell motility. (springer.com)
  • Anti-CD25 antibody-mediated depletion of effector T cell populations enhances susceptibility of mice to acute but not chronic Toxoplasma gondii inf. (nih.gov)
  • In this study using Toxoplasma gondii as a model proinflammatory infection, we have examined the capacity of anti-CD25 Abs to target effector T cell populations during an inflammatory episode, to determine to what extent that this action may modulate the outcome of disease. (nih.gov)
  • In contrast, anti-CD25 Ab treatment of mice with established chronic infections did not markedly affect brain parasite burdens, suggesting that protective T cell populations do not express CD25 during chronic stages of T. gondii infection. (nih.gov)
  • Two autoMACS® programs designed for isolation of target cells with low frequency were evaluated and adapted to bacterial biopanning, using protective antigen (PA) of Bacillus anthracis as the model system. (omicsonline.org)
  • This technique streamlines isolation of stable MP variants for rapid characterization of binding to various ligands. (rsc.org)
  • The present invention relates to tumor tropic stem cells, and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. (freepatentsonline.com)
  • 5. The isolated stem cell of claim 1, wherein said isolated stem cell comprises a heterologous gene. (freepatentsonline.com)
  • 6. The isolated stem cell of claim 5, wherein said heterologous gene encodes a polypeptide of therapeutic use in the treatment of a disease condition. (freepatentsonline.com)
  • The multiple-gene integration technique is a powerful approach for introducing and improving the biomass fermentation ability of industrial diploid S. cerevisiae strains. (biomedcentral.com)
  • a ) predominant expression in germ cells of the testis and generally not in other normal tissues, ( b ) expression in a proportion of malignant tumors of different histologic types, ( c ) expression in malignancies in a lineage nonspecific fashion, ( d ) often mapping of the gene on the X chromosome, ( e ) often members of multigene families. (aacrjournals.org)
  • In the March issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc3_10.dtl ), Christian Oker-Blom and colleagues ( www.jyu.fi/bio/bit/tutkimus_files/research.html ) provide a suite of articles detailing the use of baculovirus-based display and gene delivery systems. (cshlpress.com)
  • Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. (creative-biolabs.com)
  • Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided sufficient material to enable its biochemical characterization. (embl-heidelberg.de)
  • Akiyama SK, Yamada SS, Yamada KM (1986) Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule. (springer.com)
  • Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, McKenzie S, Broxmeyer HE, Moore MAS (1980) Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (springer.com)
  • Mitochondria are heterogeneous organelles involved in energy production, metabolism, and cellular signaling that oftentimes are isolated from cells for chemical characterization (e.g., proteomic analysis). (biomedsearch.com)
  • Ganglion cells are the final output neurons of the vertebrate retina. (utah.edu)
  • During the development of the vertebrate nervous system, many neurons become redundant (because they have died, failed to connect to target cells, etc.) and are eliminated. (embl-heidelberg.de)
  • Ciliary Neurotrophic Factor (CNTF) has been demonstrated in multiple pre-clinical models to enhance survival and regeneration of retinal ganglion cells, the retinal neurons injured in diseases like glaucoma. (stanford.edu)
  • This Review will focus on a subset of synthetic biology tools known as bottom-up techniques, which use technologies such as optogenetics and chemically induced dimerization to reconstitute cellular behavior in cells. (sciencemag.org)
  • These techniques have been crucial not only in revealing causal relationships within signaling networks but also in identifying the minimal signaling components that are necessary for a given cellular function. (sciencemag.org)
  • To validate the active cellular response to AAT deficiency in hepatocytes, we focused on Z-AAT expressing cell line models established from PI*ZZ patients or Chinese hamster ovary (CHO) cells. (ufl.edu)
  • a) An anterior-posterior cellular localization axis is exhibited by the Caulobacter histidine kinases PleC (red) and DivJ (green) that dynamically and selectively localize to specific cell poles. (nih.gov)
  • Single-cell metabolomics attempts to analyze many cellular metabolites from single cells to understand phenotypic heterogeneity, which is a significant challenge due to the low analyte abundances and limited sample volumes. (rsc.org)
  • We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). (nih.gov)
  • A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. (freepatentsonline.com)
  • The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. (freepatentsonline.com)
  • The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. (freepatentsonline.com)
  • In 1839, at age 19, he built the world's first photovoltaic cell in his father's laboratory. (wikipedia.org)
  • His research is directed at neuroprotection and regeneration of retinal ganglion cells and the optic nerve, a major unmet need in glaucoma and other optic neuropathies, and his laboratory is developing novel stem cell and nanotherapeutics approaches for eye repair. (stanford.edu)
  • Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. (asm.org)
  • The laboratory specializes in multicolor immunofluorescence analysis (up to six colors), rare event analysis, and cell separation. (cancer.gov)
  • ICON is all about transitioning scalable nanoimprinting techniques out of the laboratory and into the marketplace. (nanotech-now.com)
  • The Simmons laboratory also investigates how specialized sensory cells and their innervation patterns are refined by calcium signals during early stages of development. (baylor.edu)
  • Traxlmayr MW, Lobner E, Antes B et al (2013) Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display. (springer.com)
  • Chao G, Lau WL, Hackel BJ et al (2006) Isolating and engineering human antibodies using yeast surface display. (springer.com)
  • Moreover, mast cells themselves produce NGF, suggesting that alterations in normal mast cell behaviours can provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states. (embl-heidelberg.de)
  • [27] In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikiversity.org)
  • Bentley SA, Foidart J-M (1980) Some properties of marrow derived adherent cells in tissue culture. (springer.com)
  • TNF has been reported to increase entry of HCV pseudoparticles into hepatoma cells and inhibit signaling by interferon alpha (IFNα), but have no effect on HCV-RNA replication. (bireme.br)
  • We investigated the effects of TNF on HCV infection of and spread among Huh-7 hepatoma cells and primary HFLCs. (bireme.br)
  • RESULTS: TNF inhibited completion of the HCV infectious cycle in hepatoma cells and HFLCs in a dose-dependent and time-dependent manner. (bireme.br)
  • Using phage display techniques, a high-affinity monoclonal antibody was developed that specifically recognizes both apolipoprotein A1 (apoA1) and HDL that have been modified by the MPO-H 2 O 2 -Cl− system. (acc.org)
  • A simple design of a yeast-displayed heterodimeric Fc fragment is described and can be used as a general guideline for affinity selection of Fcabs with an asymmetric binding site. (springer.com)
  • and assessing tumor tropic potential of the stem cell based upon said expression level of CXCR4, said affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Biopanning by bacterial display has many advantages over yeast and phage display, including the speed to discovery of affinity reagents and direct amplification of bound cells without the need to elute and reinfect. (omicsonline.org)
  • For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (labx.com)
  • Surfaces displaying nano- and micropatterned cell adhesive ligands have led to numerous discoveries in cell biology. (asme.org)
  • The parent sequence is chosen based on its perceived similarity to the desired function, and a library of functional variants is generated using one of a variety of sequences diversification techniques. (openwetware.org)
  • 7. "This partnership on roll-to-roll nanoimprinting enhances our competitiveness in the global arena, particularly in helping us create new functional and patterned film technologies for displays and solar cell applications", said Mr Kim Woo Yong, Global Marketing Director of Young Chang Chemical Co. Ltd, South Korea. (nanotech-now.com)
  • Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. (illinois.edu)
  • Jonkheijm's group has also been actively developing such dynamic and responsive materials to address biomolecules, cells and even tissues. (nanowerk.com)
  • These investigations involve quantitative, single cell analyses of parameters associated with cells freshly prepared from different species/tissues as well as a wide spectrum of in vitro cultured cells. (cancer.gov)
  • Nevertheless, performance is limited by non-radiative losses, with luminescence yields in state-of-the-art perovskite solar cells still far from 100 per cent under standard solar illumination conditions. (diva-portal.org)
  • We validate these results in fully operating solar cells. (diva-portal.org)
  • Solar cells are described as being photovoltaic , irrespective of whether the source is sunlight or an artificial light. (wikipedia.org)
  • A "photoelectrolytic cell" ( photoelectrochemical cell ), on the other hand, refers either to a type of photovoltaic cell (like that developed by Edmond Becquerel and modern dye-sensitized solar cells ), or to a device that splits water directly into hydrogen and oxygen using only solar illumination. (wikipedia.org)
  • Multiple solar cells in an integrated group, all oriented in one plane, constitute a solar photovoltaic panel or module . (wikipedia.org)
  • Solar cells are usually connected in series and parallel circuits or series in modules, creating an additive voltage. (wikipedia.org)
  • The nano electronic elements can include solar cells, display elements, or antennas, among othe. (google.com)
  • This manufacturing process can easily and quickly mass produce films and surfaces with nanometer-scale textures for a host of new applications in biomedical devices, optical films, plastic electronics and flexible solar cells. (nanotech-now.com)
  • 1. Nanoimprinted structures and components are being used in items such as anti-reflection films, and solar cells. (nanotech-now.com)
  • Some of the applications that the consortium hopes to harness with roll-to-roll nanoimprint include anti-fouling surfaces, anti-reflection films to enhance the efficiency of solar cells, wire-grid polarisers, and optical films for flat panel displays. (nanotech-now.com)
  • Research into applications for carbon graphene nanosheets has focused on uses as platforms for next-wave microchips, active materials in field emitter arrays for flat panel screen displays, in biological sensors and medical imaging devices, in solar energy cells, and in high-surface area electrodes for use in bio-science. (americanelements.com)
  • He then joined the Lanekoff group as a postdoctoral fellow at Uppsala University to construct and apply ambient surface sampling mass spectrometry techniques for complex biological samples. (rsc.org)
  • The stem cells may additionally exhibit markers characteristic of astrocytic progenitors. (freepatentsonline.com)
  • The stem cells may be administered as part of a treatment regimen including the chemokine SDF-1. (freepatentsonline.com)
  • 2. The isolated stem cell of claim 1, wherein said isolated stem cell exhibits markers characteristic of a precursor for astrocytic differentiated stem cells. (freepatentsonline.com)
  • 3. The isolated stem cell of claim 2, wherein said isolated stem cell exhibits an A2B5 astrocytic precursor marker. (freepatentsonline.com)
  • 7. The isolated stem cell of claim 6, wherein said polypeptide is cytotoxic. (freepatentsonline.com)
  • 9. The isolated stem cell of claim 8, wherein said polypeptide is IL-12. (freepatentsonline.com)
  • 11. The isolated stem cell of claim 8, wherein said polypeptide is tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). (freepatentsonline.com)
  • 12. The isolated stem cell of claim 1, wherein said isolated stem cell is a neural stem cell (NSC). (freepatentsonline.com)
  • 14 . The method of claim 14, wherein said stem cell exhibits an A2B5 astrocytic precursor marker. (freepatentsonline.com)
  • 17. The method of claim 14, wherein said stem cell is a neural stem cell (NSC). (freepatentsonline.com)
  • Welcome to the Wikiversity learning project for Induced stem cells (Guide to publications). (wikiversity.org)
  • This project provides learning resources that help participants learn about Induced stem cells and efforts to produce useful stem cells and obtaining their derivatives for medical therapies. (wikiversity.org)
  • Induced stem cells (iSC) are stem cells artificially derived from somatic , reproductive , pluripotent or other cell types by deliberate w:epigenetic reprogramming. (wikiversity.org)
  • Some types of mature, specialized adult cells can naturally revert to stem cells. (wikiversity.org)
  • Another example, "chief" cells express the stem cell marker Troy. (wikiversity.org)
  • While they normally produce digestive fluids for the stomach, they can revert into stem cells to make temporary repairs to stomach injuries, such as a cut or damage from infection. (wikiversity.org)
  • Moreover, they can make this transition even in the absence of noticeable injuries and are capable of replenishing entire gastric units, in essence serving as quiescent "reserve" stem cells. (wikiversity.org)
  • This capacity to regenerate does not decline with age and may be linked to their ability to make new stem cells from muscle cells on demand. (wikiversity.org)
  • A variety of nontumorigenic stem cells display the ability to generate multiple cell types. (wikiversity.org)
  • For instance, multilineage-differentiating stress-enduring (Muse) cells are stress-tolerant adult human stem cells that can self-renew. (wikiversity.org)
  • 4 5 Although thrombopoietin (TPO) plays a major role in regulating MK and platelet production, 6-8 many studies point to additional contributions from stem cell factor (SCF), interleukin-3 (IL-3), interleukin-6 (IL-6), interleukin-11 (IL-11), granulocyte-macrophage colony-stimulating factor (GM-CSF), and basic fibroblast growth factor (bFGF). (bloodjournal.org)
  • Megakaryocyte (MK) progenitor cells proliferate, polyploidize, increase in size, and develop lineage-specific cell surface and cytoplasmic markers. (bloodjournal.org)
  • Since we have done this in bacterial cells, in principle, we can grow an infinite amount of cells with these supramolecular properties,' Jonkheijm points out. (nanowerk.com)
  • Other researchers developed it decades ago, using yeast or bacterial cells, but the existing method hasn't worked to make and test diverse CDPs, Crook said. (fredhutch.org)
  • Bentley SA, Tralka TS (1983) Fibronectin-mediated attachment of hematopoietic cells to stromal elements in continuous bone marrow culture. (springer.com)

No images available that match "cell surface display techniques"