A proteolytic enzyme obtained from Streptomyces griseus.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.
A field of medicine concerned with developing and using strategies aimed at repair or replacement of damaged, diseased, or metabolically deficient organs, tissues, and cells via TISSUE ENGINEERING; CELL TRANSPLANTATION; and ARTIFICIAL ORGANS and BIOARTIFICIAL ORGANS and tissues.
Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
Methods for maintaining or growing CELLS in vitro.
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The physiological renewal, repair, or replacement of tissue.
Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Polymorphic cells that form cartilage.
The process of bone formation. Histogenesis of bone including ossification.
Transference of cells within an individual, between individuals of the same species, or between individuals of different species.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
A type of CARTILAGE characterized by a homogenous amorphous matrix containing predominately TYPE II COLLAGEN and ground substance. Hyaline cartilage is found in ARTICULAR CARTILAGE; COSTAL CARTILAGE; LARYNGEAL CARTILAGES; and the NASAL SEPTUM.
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Devices for simulating the activities of the liver. They often consist of a hybrid between both biological and artificial materials.
The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.
A type of CARTILAGE whose matrix contains large bundles of COLLAGEN TYPE I. Fibrocartilage is typically found in the INTERVERTEBRAL DISK; PUBIC SYMPHYSIS; TIBIAL MENISCI; and articular disks in synovial JOINTS. (From Ross et. al., Histology, 3rd ed., p132,136)
Cells that can give rise to cells of the three different GERM LAYERS.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.
Cells with high proliferative and self renewal capacities derived from adults.
The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.
Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS).
A TGF-beta subtype that plays role in regulating epithelial-mesenchymal interaction during embryonic development. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta3 and TGF-beta3 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor.
Jelly-like connective tissue of the UMBILICAL CORD that contains MESENCHYMAL STROMAL CELLS.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.
Centers for collecting, characterizing and storing human blood.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.
The sum of the weight of all the atoms in a molecule.
Transplantation of an individual's own tissue from one site to another site.
Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.
Thin outer membrane that surrounds a bone. It contains CONNECTIVE TISSUE, CAPILLARIES, nerves, and a number of cell types.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Established cell cultures that have the potential to propagate indefinitely.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)
Submicron-sized fibers with diameters typically between 50 and 500 nanometers. The very small dimension of these fibers can generate a high surface area to volume ratio, which makes them potential candidates for various biomedical and other applications.
Flaps of tissue that prevent regurgitation of BLOOD from the HEART VENTRICLES to the HEART ATRIA or from the PULMONARY ARTERIES or AORTA to the ventricles.
A mutant strain of Rattus norvegicus without a thymus and with depressed or absent T-cell function. This strain of rats may have a small amount of hair at times, but then lose it.
A product from the iodination of tyrosine. In the biosynthesis of thyroid hormones (THYROXINE and TRIIODOTHYRONINE), tyrosine is first iodized to monoiodotyrosine.
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
A strong oxidizing agent.
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
A superorder of CEPHALOPODS comprised of squid, cuttlefish, and their relatives. Their distinguishing feature is the modification of their fourth pair of arms into tentacles, resulting in 10 limbs.
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
Elements of limited time intervals, contributing to particular results or situations.
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Prosthesis, usually heart valve, composed of biological material and whose durability depends upon the stability of the material after pretreatment, rather than regeneration by host cell ingrowth. Durability is achieved 1, mechanically by the interposition of a cloth, usually polytetrafluoroethylene, between the host and the graft, and 2, chemically by stabilization of the tissue by intermolecular linking, usually with glutaraldehyde, after removal of antigenic components, or the use of reconstituted and restructured biopolymers.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A mitosporic Tremellales fungal genus whose species usually have a capsule and do not form pseudomycellium. Teleomorphs include Filobasidiella and Fidobasidium.
... or pronase, before agitating the tissue to release the cells into suspension. Alternatively, pieces of tissue can be placed in ... use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ...
Cell yield after enzymatic digestion of the nasal cartilage was estimated to be 2,100 - 3,700 cells/mg of tissue [7] [12]. ... a potent source for stem cell-based regenerative medicine. Stem Cells Dev 20:2077-2091. Tay AG, Farhadi J, Suetterlin R, Pierer ... alone or after an initial short pre-incubation phase with pronase (0.2% - 1%) [7][8][9][10][11][12][13]. ... Cell Tissue Bank 14:255-266. Shafiee A, Kabiri M, Ahmadbeigi N, Yazdani SO, Mojtahed M, Amanpour S, Soleimani M 12/2011 Nasal ...
Major commercial sources Source Inhibitor Molecular weight Inhibitory power Details Blood plasma α1-antitrypsin 52 kDa Also ... TATI sensitivity in the setting of renal cell carcinoma is approximately 70%. Elevated TATI is more likely to be seen in ... This article includes a list of references, but its sources remain unclear because it has insufficient inline citations. Please ... and renal cell carcinoma. TATI is metabolised by the kidneys and is, thus, elevated in patients with renal failure. It may be ...
Parietal cells: Produce hydrochloric acid and intrinsic factor.. *Gastric chief cells: Produce pepsinogen. Chief cells are ... This section does not cite any sources. Please help improve this section by adding citations to reliable sources. Unsourced ... Find sources: "Digestive enzyme" - news · newspapers · books · scholar · JSTOR (December 2016) (Learn how and when to remove ... Find sources: "Digestive enzyme" - news · newspapers · books · scholar · JSTOR (December 2016) (Learn how and when to remove ...
positive regulation of cell migration. • blood coagulation. • proteolysis. • ER to Golgi vesicle-mediated transport. • blood ... Traditional models of coagulation developed in the 1960s envisaged two separate cascades, the extrinsic (tissue factor (TF)) ... self-published source][9] ... Factor X has been shown to interact with Tissue factor pathway ... In stage 1, Factor VII binds to the transmembrane protein TF on the surface of cells and is converted to Factor VIIa. The ...
Find sources: "Cell wall" - news · newspapers · books · scholar · JSTOR (November 2017) (Learn how and when to remove this ... Esau's Plant Anatomy: Meristems, Cells, and Tissues of the Plant Body: Their Structure, Function, and Development. 3rd.ed. John ... while the innermost is sensitive to cellulase and pronase.[35] ... Rigidity of cell wallsEdit. In most cells, the cell wall is ... Cells with secondary cell walls can be rigid, as in the gritty sclereid cells in pear and quince fruit. Cell to cell ...
... inducing cell death". Molecular Cell. 8 (3): 613-21. doi:10.1016/S1097-2765(01)00341-0. PMID 11583623.. ... Analysis of biopsy samples showed changes in expression of HtrA2 in cancer tissues compared with normal tissues. ... cell nucleus. • serine-type endopeptidase complex. Biological process. • cellular response to retinoic acid. • cellular ... "A novel mechanism for imatinib mesylate-induced cell death of BCR-ABL-positive human leukemic cells: caspase-independent, ...
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three- ... the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. ... Briefly, stromal cells, derived from either the patients tumor or normal tissue or from an allogeneic source, are established ... These include but are not limited to trypsin, chymotrypsin, collagenase, elastase, and/or hyaluronidase, Dnase, pronase, ...
As assessed by cell transfer, lymphoid cells from PP and APP appear to be highly enriched sources of IgA plasma cell precursors ... these Ig did not appear to be products of the cells which bore them as they were not regenerated after removal of Pronase. It ... with respect to the origin of IgA plasma cells and the postulated bursal role of mammalian gut-associated lymphoid tissue. ... When the cells were stained directly for µ chain determinants, about 33% of PLN cells and 28% of PP cells were positive. These ...
It is a fibrous protein and functions primarily as a supporting tissue and scaffolding for other proteins and cells. It is ... The method of obtaining the collagen from the crude collagen source, e.g. skin, tendon, hide, etc., by enzyme extraction is as ... pronase, proctase, etc. The soluble extract is worked up as follows: The solution is brought to a pH of about 10.0 (when ... 2) It shows good affinity with cells and works as a base for the production or proliferation of cells and further is effective ...
... and desmosomes are intercellular junctions in vertebrate epithelial cells, thus indicating that N-glycosylation of tissue- ... LGs are a major source of stratum corneum lipid precursors and various hydrolytic enzymes such as lipases, proteases, acid ... Pronase, Calbiochem. α-l-Fucosidase from bovine kidney and other chemical reagents were obtained from Wako Pure Chemical ... The secretory granular cell: the outermost granular cell as a specialized secretory cell. J. Investig. Dermatol. Symp. Proc. 3 ...
Tissue inhibitor of metalloproteinase (TIMP) secretion by pancreatic stellate cells (PSCs). (A) TIMP1 and TIMP2 activity was ... pronase (0.02%), and DNase (0.1%) in Geys balanced salt solution. The resultant suspension of cells was centrifuged in a 13.2 ... concentrated conditioned medium of HT1080 cells (as a source of gelatinases). After electrophoresis, gels were washed twice in ... quiescent cells) or 48 hours (activated cells). The medium was then changed to IMDM with 0.2% FBS and cells incubated for a ...
This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines. It ... This unique fungus can degrade keratin (hence Proteinase "K") and can grow using the by-product as its sole source of carbon ... 6x greater activity than Pronase and 3x greater activity than bovine trypsin. • Broad substrate specificity. • Versatile in ... Cell Biology/Cell Culture. Diagnostics. Cancer Research. Pharmaceuticals. Medical Device. Food & Beverage. Environmental. ...
... smooth muscle cells, cardiac muscle cells, connective tissue cells, glial cells, epithelial cells, endothelial cells, hormone- ... obtained from mammalian sources, including bovine, porcine and human sources. Human sources include patients and cadavers. ... chondrocytes are isolated by sequential enzymatic digestion of full-thickness articular cartilage with pronase and collagenase ... Cells useful in the methods of the invention include stem cells, somatic cells, committed stem cells, differentiated cells, and ...
... tissue cells, tumor cells and the like. Representative periodate solutions include sodium periodate at a concentration of about ... or degradative products of these cells, (e.g. by size or fluorescence intensity), thereby to identify the microbial source ( ... Representative proteases solutions include trypsin at about 0.1% w/v to about 1% w/v, pronase at about 0.25% to about 2% w/v, ... Recombinant host cells include bacterial cells, insect cells, yeast cells and mammalian cells. The subject host cell ...
ECs were isolated from human umbilical cord veins by pronase perfusion as previously described26 ; seeded on 0.2% (wt/vol) ... gelatin-coated, 96-well microtiter tissue-culture plates; and used at confluence, which was typically reached after 4 to 5 days ... HBDs of adhesive GPs involved in mediating cell-cell and cell-matrix interactions include FN,8 9 10 laminin,11 12 vitronectin, ... 33 35 Many adhesive GPs involved in cell-cell and cell-matrix interactions, including fibrin(ogen), have both HBDs8 9 10 11 12 ...
Cell uptake studies.. On day 11, uptake of radiolabeled substrate by cells plated onto 24-well tissue culture plates was ... and 1 mg/ml Pronase and 0.5 mg/ml DNAse I (Boehringer-Mannheim, Indianapolis, IN) (Crook et al., 1981). Cell dispersion was ... Louis, MO). All other chemicals were obtained from commercial sources and were of the highest grade available. ... Fixation and cell processing for immunofluorescent staining.. Cells were isolated as described above and plated on glass tissue ...
These results show that the influx of monocytes is the source of cell renewal for the pulmonary macrophages. No indications for ... in the steady state were isolated by lavage with PBS containing EDTA and subsequent enzymatic digestion of tissue with pronase ... These receptors were almost absent on PAM and present on a larger number of cells in the mixed population of PAM and PTM. The ... By this method, the total pulmonary macrophage population was obtained in two cell suspensions, one with a pure population of ...
CHAPTER 6 - DISSOCIATED CELL CULTURE. (Source: D. Frost and D. Sepich) Preparing Embryos for Cell Culture. Treat embryos with ... 3. Plate onto tissue culture dish in a single drop of medium with 5% serum (to help attachment).. 4. Incubate 4 hr at 28.5°C.. ... Remove the chorions with watchmakers forceps or by soaking the embryos in pronase (see Removing Embryos from Chorions, Chapter ... CHAPTER 6 - DISSOCIATED CELL CULTURE. Contents. Preparing Embryos for Cell Culture. 0 Ca2+ - Mechanical Dissociation. Protease ...
The tissue was then incubated with Pronase (750 U/ml, 50 min at 22°C; Boehringer Mannheim) dissolved in artificial sea water ( ... but cell-to-cell variability in time constant was encountered: although for most cells of both types τ = 2 ms, there were ∼15% ... Low-medium intensity illumination for eliciting responses in the physiological range was produced by one of two sources. (1) A ... Open symbols, Joseph cells (n = 5-9 per point). Filled symbols, Hesse cells (n = 4-5 per point). Error bars indicate SEM. C, ...
Optimisation of isolation yields, abundant NVP-BGJ398 cell signaling cell sources and efficient culturing procedures that lead ... NVP-BGJ398 cell signaling The state-of-the-art idea of cartilage tissues advancement combines the usage of biodegradable and ... pronase, hyaluronidase etc.) isnt uncommon (Jakob et al., 2001). Many types of effective chondrocyte isolation techniques ... and ACAN offers been shown how the proposed protocol potential clients to isolation of cells with a NVP-BGJ398 cell signaling ...
Liver tissue was obtained on days 1, 3, 5, and 8 for assessment of Kupffer cell numbers, early fibrogenic events and LT-β gene ... Kupffer cells were isolated using pronase/collagenase perfusion and centrifugal elutriation.. Results: Hepatic LT-β mRNA ... of bile duct ligation liver injury whether LT-β expression is induced and whether Kupffer cells are an intrahepatic source of ... Methods: Sprague-Dawley rats were divided into two groups: one group received a single dose of GdCl (a Kupffer cell-blocking ...
Cell cycle evaluation Cells had been treated with unique concentr. Posted on July 25, 2014. by mtor0294 ... Only tissue from donors without a historical past of recognized arthritis was made use of.. The tissue was graded on a scale of ... Antibodies and their sources had been as follows insulin receptor substrate 312 and complete was from Upstate Biotechnol ogy, ... Pronase was from Cal biochem. DMEMHams F twelve, antibiotics, fetal bovine serum, and PicoGreen double stranded DNA assay ...
... these cells present an abundant cell source for meniscus tissue engineering. ... also indicated that sequential digestion of meniscus tissue with pronase and collagenase was able to yield more cells with ... meniscus cells were characterized biomechanically and an effective method for isolating these cells for tissue engineering was ... this thesis also investigated the use of skin cells as an alternative to primary cells for tissue engineering. Previously ...
These results show that several proteasome inhibitors do not protect normal cells and tissues in the developing fish larvae ... In select experiments, embryos (24 hpf) were dechorionated by placement in embryo medium supplemented with 50 μg/mL pronase ( ... or a 137Cs radiation source. Toxicity analyses for EP (,10 mmol/L), CDDO-TFEA (,10 μmol/L), PS-341 (,10 μmol/L), MG132 (,50 ... HeLa cells were seeded at 7.5 × 104/mL in DMEM supplemented with 10% fetal bovine serum. The cells were cotransfected with the ...
... tissue cells, in or on a nutrient medium. As is well known to those of skill in the art of cell or tissue culture, a cell ... Cells may be isolated from a number of sources, including, for example, biopsies from living subjects and whole-organ recover ... These include but are not limited to trypsin, chymotrypsin, collagenase, elastase, and/or hyaluronidase, DNase, pronase and ... Alternatively, cells obtained from cell culture may be lifted from a culture plate as a cell layer, and the cell layer may be ...
Heating the cells to 65°C abolished AAG activity (Table 2). The AAG activity of strain 81-176 was abolished by pronase or acid- ... Trypsinized cells were placed in a 24-well tissue culture plate (Costar) and cultivated at 37°C for 24 h in 5% CO2. Immediately ... or meat products and direct contact with infected pets are considered the most important sources of infection in humans (3, 53 ... Adherence to INT407 cells.INT 407 cells (human embryonic intestine cells [ATCC CCL 6]) were obtained from the American Type ...
... or pronase, before agitating the tissue to release the cells into suspension. Alternatively, pieces of tissue can be placed in ... use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ...
Adhesion of type A Pasteurella mulocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections ... attached to squamous epithelial cells of the pharyngeal mucosa both in vivo and in vitro and to some tissue culture cell lines ... Source. PMC Keywords. * Research Article Abstract. Pasteurella multocida serotype A was found in association with the mucosal ... Bacterial adhesiveness was much reduced after pronase digestion, heat treatment, and homogenization, but removal of the ...
... includes cells present in a suspension of cells isolated from a vertebrate tissue source and cultured, or it can refer to the ... If enzymatic digestion is used, enzymes such as collagenase, hyaluronidase, dispase, pronase, trypsin, elastase and ... renal tissues, bone tissues, intestinal tissues, nerve tissues, hepatic tissues, dermal tissues, epidermal tissues, or the like ... renal tissues, bone tissues, intestinal tissues, nerve tissues, hepatic tissues, dermal tissues, epidermal tissues, or the like ...
... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... Satellite Cell Isolation and Culture. Primary satellite cells were obtained from plantaris muscles digested with pronase as ... Skeletal muscle represents a plentiful and accessible source of adult stem cells. Skeletal-muscle-derived stem cells, termed ...
The neocartilage compositions are useful as implants and as replacement tissue for damaged or defective cartilage and as a ... said cells being surrounded by a substantially continuous insoluble glycosaminoglycan and collagen-enriched hyaline ... Disclosed are neocartilage compositions characterized by having multiple layers of cells, ... The sources of various materials used in the specific laboratory examples are as follows:. MATERIALS. Dulbeccos modified ...
The majority of both articular and costal chondrocytes were observed to be organelle-rich round to oval cells embedded in ... The present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. ... these data suggest that the costal chondrocytes could provide a beneficial and more accessible source of chondrocytes for three ... The propagation patterns of both cell types were similar, undergoing proliferation during the first 40 days and almost ceasing ...
The antibody PG-M1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. Among ... DE000, DE006, and DE007). Unmasking with Pronase (Art. No. DE110) or Proteinase K is also suitable. ... Source. Immunogen: Gaucher cells Product. Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium ... Pre-Treatment: Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C, E or G (Art. No. ...
Cells were then seeded onto 100-mm fibronectin-coated tissue culture dishes and grown at 37°C in 3.5% CO2 until 80% confluent. ... In addition, BECs from never-smokers were obtained from two different sources: (a) cells derived at autopsy by Clonetics, Inc ... DNA was isolated from BECs, tumors, and sputum by digestion with Pronase in 1% SDS, followed by standard phenol-chloroform ... Normal human tissue collected from autopsy of never-smokers and cell lines positive for p16 (Calu6), MGMT (SkLU1), DAP kinase( ...
Glycopeptides were prepared from a Triton X-100 extract of the cells by pronase digestion. ... The fluorescence in tumour tissue was twice that of normal urothelium in vitro and up to tenfold in vivo. Residual disease at ... In the era of big data, information regarding the same objects can be collected from increasingly more sources. ... We were unable to correlate T-cell functional response in T-cell colony formation with the phenotypic profile of peripheral ...
Germ Cell Transplantation and Testis Tissue Xenografting in Mice. Authors: Lin Tang, Jose Rafael Rodriguez-Sosa, Ina Dobrinski. ... Such assays are of two major types - assays that quantitate sperm movement to a source of chemoattractant, so-called sperm ... elegans spermatids using Pronase E. Successful spermiogenesis is pre-requisite for fertility and hence the mutants defective in ... The cell-permeant AM (acetoxy methyl ester) ester form of the Ca2+-reporting dye is then added to a cell aliquot and a period ...
  • The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. (google.com)
  • The role of pancreatic stellate cells in extracellular matrix formation and fibrogenesis may be related to their capacity to regulate the degradation as well as the synthesis of extracellular matrix proteins. (bmj.com)
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (wikipedia.org)
  • Furthermore, the molecular mechanisms by which exercise-stimulating extracellular factors control the satellite cell activation and differentiation remain unclear. (hindawi.com)
  • Physical exercise induces changes in extracellular signaling in skeletal muscle that affect satellite cells. (hindawi.com)
  • 2]. The remaining cartilaginous part (characterized as hyaline cartilage) of the human nasal septum has a specific three-dimensional organization with regards to local differences in cell size and the amounts of extracellular matrix. (wikipedia.org)
  • Since NC can also express hyaline cartilage specific extracellular matrix proteins such as glycosaminoglycans and collagen, NC have recently also been used for the in vitro engineering of cartilage tissues. (wikipedia.org)
  • The link of high cytosolic glucose concentration to metabolic dysfunction was demonstrated by overexpression of the GLUT1 glucose transporter in renal mesangial cells that thereby acquired the characteristics of the diabetic phenotype, including increased extracellular matrix protein synthesis and activation of the polyol pathway ( 7 ). (diabetesjournals.org)
  • Integrins are a family of cell surface receptors that form cell-cell and cell-extracellular matrix contacts and, as such, are key targets in treating fibrotic disease. (sciencemag.org)
  • Integrins are present in nearly all multicellular organisms and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity ( 1 ). (sciencemag.org)
  • Hepatic stellate cells (HSCs) play a crucial role in liver fibrosis, which is a pathological process characterized by extracellular matrix accumulation. (peerj.com)
  • 16. The method of claim 15, wherein the pPS cells are plated on a solid surface without any extracellular matrix. (patentsencyclopedia.com)
  • Thrombospondins (TSPs) are a family of extracellular proteins recognized as an endogenous constituent of the extracellular matrix (ECM) in many human tissues. (biomedcentral.com)
  • The extracellular matrix (ECM) is an important factor influencing cell fate. (biologists.org)
  • It is a major component of the extracellular matrix (ECM) and normally present as high molecular mass (several thousand kDa) in mammalian bone marrow and loose connective tissues ( Laurent and Fraser, 1992 ). (biologists.org)
  • Furthermore, disc cells actively regulate the homeostasis of the extracellular matrix by several cytokines and growth factors acting in an autocrine and paracrine fashion. (biomedcentral.com)
  • Variation of extracellular oxygen concentrations influences cellular metabolism and is key to tissue development and function. (spiedigitallibrary.org)
  • Weight loss alone underestimated the sum of their fat and lean tissue losses, because their extracellular fluid volume increased. (signali-forex.com)
  • Spreading of human umbilical vein endothelial cells (ECs) on fibrin requires thrombin cleavage of fibrinopeptide B (FPB) and subsequent exposure of the new β15-42 N -terminus. (ahajournals.org)
  • A variety of cells such as endothelial cells, hepatic stellate cells (HSCs) and Kupffer cells are involved in liver fibrogenesis. (peerj.com)
  • Hepatic stellate cells (HSCs) exist in the space between parenchymal cells and liver sinusoidal endothelial cells of the hepatic lobule. (mdpi.com)
  • To gain insight into cellular regulatory mechanisms that determine hepatic synthesis of IGF-I and IGFBPs and to identify potential target cells for IGF-I within the liver, we studied the cellular sites of synthesis of IGF-I, IGF receptor, growth hormone (GH) receptor, and IGFBPs in freshly isolated rat hepatocytes, endothelial cells, and Kupffer cells. (physiology.org)
  • Type 1 IGF receptor mRNA was detected in endothelial cells, indicating that these cells are a local target for IGF actions in liver. (physiology.org)
  • IGFBP-1 was synthesized only in hepatocytes, and IGFBP-3 was expressed in Kupffer and endothelial cells. (physiology.org)
  • IGFBP-4 was expressed at high levels in hepatocytes and at low levels in Kupffer and endothelial cells. (physiology.org)
  • In the present study, we compared the levels of expression of type 1 IGF receptor mRNA in RNA extracted from whole adult rat liver and from freshly isolated hepatocytes, Kupffer cells, and endothelial cells prepared from adult liver. (physiology.org)
  • A significant regenerative potential is due to the presence of stem cells in the endometrium, such as mesenchymal, epithelial and endothelial progenitor cells [9]. (exp-oncology.com.ua)
  • Liver nonparenchymal cells including sinusoidal endothelial cells and Kupffer cells constitutively expressed PD-L1 and inhibited proliferation and cell division of activated T cells expressing PD-1. (rupress.org)
  • Advanced glycation of the Arg-Gly-Asp (RGD) tripeptide motif modulates retinal microvascular endothelial cell dysfunction. (biomedsearch.com)
  • PURPOSE: Advanced glycation endproduct (AGE) formation on the basement membrane of retinal capillaries has been previously described but the impact of these adducts on capillary endothelial cell function vascular repair remains uncertain. (biomedsearch.com)
  • This investigation has evaluated retinal microvascular endothelial cells (RMECs) growing on AGE-modified fibronectin (FN) and determined how this has an impact on cell-substrate interactions and downstream oxidative responses and cell survival. (biomedsearch.com)
  • Arginine-specific modifications alter vital endothelial cell interactions with the substrate. (biomedsearch.com)
  • brooker Introduction The interactions and responses of cells (macrophages, ;ip, fibroblast and endothelial cells) to foreign metal elements have been widely investi- gated in the past decades. (forexicreg.com)
  • Permeability of frog mesenteric capillaries after partial pronase digestion of the endothelial glycocalyx. (newnetmediamarketing.com)
  • Our comprehensive endothelial cell culture portfolio consists of 12 different types of large vessel and microvascular human endothelial cells with optimized growth media for each cell type. (newnetmediamarketing.com)
  • Manual MSDS × Selected product Human Cardiac Microvascular Endothelial Cells (HCMEC) cryopreserved 500,000 cells. (newnetmediamarketing.com)
  • Endothelial progenitor cells (EPCs) and mesenchymal stem/stromal cells (MSCs) are associated with maintaining tissue homeostasis and tissue repair. (newnetmediamarketing.com)
  • Tumor-associated endothelial cells expressed typical endothelial cell markers, such as CD31. (newnetmediamarketing.com)
  • Garipcan and his team found iliac artery endothelial cells to be 13.2 ± 4.1 μm in width and 25.8 ± 8.5 μm in length (Garipcan et al. (newnetmediamarketing.com)
  • Tumour angiogenesis is a complex process based upon a sequence of interactions between tumour cells and endothelial cells. (newnetmediamarketing.com)
  • Brain endothelial cells have unique properties in terms of barrier function, local molecular signaling, regulation of local cerebral blood flow (CBF) and interactions with other members of the neurovascular unit. (newnetmediamarketing.com)
  • Here, we used 7 cell type-specific mouse Cre lines to conditionally knock out Vps33b in Cdh5 + or Tie2 + endothelial cells (ECs), Lepr + BM perivascular cells, Osx + osteoprogenitor cells, Pf4 + megakaryocytes, and Tcf21 + spleen stromal cells. (newnetmediamarketing.com)
  • Deficiency of HSPA12B … Mouse endothelial cells were isolated from two different human tumor xenografts, melanoma and liposarcoma, and from two normal endothelial cell counterparts, skin and adipose. (newnetmediamarketing.com)
  • However, they described the width of hepatic artery endothelial cells as 4.9 ± 1.5 μm and 21.9 ± 6.6 μm in length. (newnetmediamarketing.com)
  • Endothelial progenitor cell (or EPC) is a term that has been applied to multiple different cell types that play roles in the regeneration of the endothelial lining of blood vessels.Outgrowth endothelial cells are an EPC subtype committed to endothelial cell formation. (newnetmediamarketing.com)
  • To test this hypothesis, four endothelial cell lines derived from different organs were chosen as endothelial cell models: hCMEC/D3 (brain), HPMEC-ST1.6R (lung), TRP3 (liver), and ciGENC (kidney). (newnetmediamarketing.com)
  • Gene expression markers for Endothelial cells. (newnetmediamarketing.com)
  • This also applies to endothelial cells in capillaries. (newnetmediamarketing.com)
  • Mouse Anti CD34 Endothelial Cell Marker. (newnetmediamarketing.com)
  • Read our blog post about how scientists are using human umbilical vein endothelial cells in cancer research. (newnetmediamarketing.com)
  • Lamellar granules are organelles found in the terminally differentiating cells of keratinizing epithelia, and desmosomes are intercellular junctions in vertebrate epithelial cells, thus indicating that N -glycosylation of tissue-specific glycoproteins may contribute to increase the relative proportion of high mannose glycans. (mcponline.org)
  • The objective of this study was to develop and evaluate a primary culture system for choroid plexus epithelial cells as an in vitro model for studying organic cation transport. (aspetjournals.org)
  • The objective of this study was to evaluate the morphological and physiological properties of a primary culture system of choroid plexus epithelial cells and its capacity to serve as an in vitro model to access the cellular mechanisms of organic cation transport across the CSF-blood barrier. (aspetjournals.org)
  • The bacteria specifically attached to squamous epithelial cells of the pharyngeal mucosa both in vivo and in vitro and to some tissue culture cell lines such as HeLa. (mysciencework.com)
  • The purpose of our study was to determine the prevalence for aberrant promoter methylation of the p16 , O 6 -methylguanine-DNA methyltransferase (MGMT), death-associated protein (DAP) kinase , and Ras effector homologue (RASSFIA) genes in nonmalignant bronchial epithelial cells from current and former smokers in a hospital-based, case control study of lung cancer. (aacrjournals.org)
  • In pre-implantation development, trophoblast cells are derived from trophectoderm, the external epithelial layer of the blastocyst. (biologists.org)
  • Streptococcus pneumoniae induced IL-10 expression in mouse lungs and human lung epithelial cells. (ersjournals.com)
  • Bile duct isolates free of HSC contaminants, form small cell clusters, which initially comprise a large majority of epithelial cells. (frontiersin.org)
  • In culture conditions (fetal bovine serum) that provide a growth advantage to mesenchymal cells over epithelial cells, the epithelial cells die and detach from the substrate, while spindle-shaped cells outgrow from the periphery of the cell clusters, as shown by video-microscopy. (frontiersin.org)
  • We found no evidence for epithelial-mesenchymal transition, i.e., no co-expression of alpha-smooth muscle actin and cytokeratin at any stage, while cytokeratin becomes undetectable in the confluent cells. (frontiersin.org)
  • Elimination of changes in mitochondrial respiration during stress by negative aero-ions Type-5 endings are branching dendrites of skin receptors cells that terminate among epithelial cells. (storysteel.cf)
  • In addition, continuous cultures of porcine turbinate cells (PT-K75) and primary chicken embryo epithelial kidney cells supported SARS-CoV replication. (cdc.gov)
  • TSP-1 was detected in perivascular tissue, at the epithelial-stromal junction, in the stroma and in tumour cells in the majority of the cases. (biomedcentral.com)
  • In two calves with caseonecrotic pneumonia, necrotic foci were surrounded by epithelial cells resembling bronchial or bronchiolar epithelium. (biomedcentral.com)
  • Primary population of endometrial cells was heterogeneous and contained cells with fibroblast-like and epithelial-like morphology, but at P3 the majority of cell population had fibroblast-like morphology. (exp-oncology.com.ua)
  • The relative expression stability of commonly used housekeeping genes were determined in primary human bronchial epithelial cells (HBECs), pig tracheal epithelial cells (PTECs), and chicken and duck primary lung-derived cells infected with five influenza A virus subtypes. (biomedcentral.com)
  • Based on the morphological, biochemical and functional properties of these cultured cells, we conclude that this primary culture system should be an excellent in vitro model for experimental characterization of choroid plexus function. (aspetjournals.org)
  • In labeling experiments with 3H-thymidine, the low in vitro labeling indices (less than 3%) for both PAM and the mixture of PAM and PTM, showed that both are essentially nondividing cells. (rupress.org)
  • To determine if allogeneic or xenogeneic implantation of scaffold-free meniscus constructs could be feasible, the immunogenicity of bovine and leporine meniscus cells and articular chondrocytes were determined in an in vitro model system. (rice.edu)
  • Furthermore, ethyl pyruvate (EP), a derivative of the end product of glycolysis, similarly protects normal cells against the deleterious effects of radiation both in vitro and in mice ( 12 ). (aacrjournals.org)
  • Alternatively, NC can be isolated by outgrowth culture of nasal cartilage fragments[8] After isolation from septal cartilage biopsies, NC can be extensively expanded in numbers by conventional in vitro cell culture methods (monolayer culture in flasks or Petri dishes). (wikipedia.org)
  • The search for the cellular basis of trophoblast proliferation in post-implantation development was addressed with an in vitro spatial and temporal analysis of fibroblast growth factor 4-dependent trophoblast stem cell potential. (biologists.org)
  • Coincident with occlusion of the ectoplacental cavity via union between chorionic ectoderm and the ectoplacental cone, a decline in the frequency of mitotic chorionic ectoderm cells in vivo, and of trophoblast stem cell potential in vitro, was observed. (biologists.org)
  • Therefore, progenitor cells which can give rise to TSCs in vitro, appear to exist until at least the onset of gastrulation. (biologists.org)
  • The inventors have now developed a novel cell-based tissue engineering approach to the treatment ' of rotator cuff tear comprising the implantation of a bioscaffold that has been seeded with tenocytes which have been expanded in vitro. (lens.org)
  • Finally, we show in vitro that the interaction of NK cells with F. nucleatum leads to an NCR1-dependent secretion of TNF-α. (pubmedcentralcanada.ca)
  • We aimed to study biological properties of human endometrial stromal cells in vitro . (exp-oncology.com.ua)
  • They can be easily isolated from the bone marrow, adipose tissue, umbilical cord, dental pulp, and extra-embryonic tissues and can be successfully expanded in vitro [2-7]. (exp-oncology.com.ua)
  • PD-1 is induced on T cells, B cells, and myeloid cells upon activation in vitro ( 9 ), but predominantly expressed on activated T cells in vivo ( 10 ). (rupress.org)
  • The objective of this study was to identify nucleus pulposus-derived progenitor cells (NPPC) and examine their potential in regenerative medicine in vitro. (biomedcentral.com)
  • The obtained Tie2- and Tie2+ fractions of cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages in vitro. (biomedcentral.com)
  • NaAsO 2 treatment significantly enhanced the ROS-mediated oxidative stress in the hepatic cells both in in vitro and in vivo systems. (hindawi.com)
  • The effects of vorinostat on proliferation, induction of apoptosis and cell cycle effects were studied in vitro (D54, U87 and U373 glioma cell lines). (termsreign.ga)
  • Eissenstat, M. F Best Strategy Finance Ltd. 0 pip spread forex broker applied in vitro for 1h in appropriate doses, caused irreversible inhibition of leukemic L1210 cell proliferation. (forexicreg.com)
  • Pulmonary macrophages of mice in the steady state were isolated by lavage with PBS containing EDTA and subsequent enzymatic digestion of tissue with pronase and DNA-ase. (rupress.org)
  • By this method, the total pulmonary macrophage population was obtained in two cell suspensions, one with a pure population of pulmonary alveolar macrophages (PAM) and the other with a mixed population of pulmonary alveolar and pulmonary tissue macrophages (PTM). (rupress.org)
  • The morphological, cytochemical, and functional characteristics of both PAM and PTM were like those of mature tissue macrophages except for the presence of C3 receptors. (rupress.org)
  • These results show that the influx of monocytes is the source of cell renewal for the pulmonary macrophages. (rupress.org)
  • The antibody PG-M1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. (exalpha.com)
  • KP-1 is important for identifying macrophages in tissue sections. (exalpha.com)
  • It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. (exalpha.com)
  • In M. bovis infected calves, hyperplasia of bronchus-associated lymphoid tissue (BALT) was characterized by strong MHC class II expression of lymphoid cells, but only few of the macrophages demarcating the caseonecrotic foci were positive for MHC class II. (biomedcentral.com)
  • Although isolated alveolar macrophages appeared to express PAR 1 - and PAR 4 -immunoreactivity, these cells failed to release TNF- α above baseline levels in response to thrombin, trypsin or any of the peptide PAR agonists. (wiley.com)
  • We examined whether human liver macrophages (LMs), the largest tissue macrophage population, comprise an HIV-1 reservoir. (jci.org)
  • Apolipoprotein E (apoE) is a 34-kDa protein that is produced and secreted by many cell types such as hepatocytes, smooth muscle cells, neuronal cells, and macrophages. (ahajournals.org)
  • Such 'virus-transporting' macrophages would be in a position to carry infectious FMDV to different sites in the body, where it could be released to infect other cells for replication. (nih.gov)
  • We have demonstrated that massive in vivo targeting of liposomes to a defined cell population other than macrophages is possible. (newnetmediamarketing.com)
  • Cells were dispersed from choroid plexus of neonatal rats by enzymatic digestion and grew as differentiated monolayers when plated on solid or permeable support. (aspetjournals.org)
  • A comparison of isolation techniques also indicated that sequential digestion of meniscus tissue with pronase and collagenase was able to yield more cells with higher viability than other techniques tested, and those isolated cells created stiffer and more glycosaminoglycan (GAG) rich constructs when used in a tissue engineering modality than cells isolated using only collagenase. (rice.edu)
  • Bacterial adhesiveness was much reduced after pronase digestion, heat treatment, and homogenization, but removal of the hyaluronic acid capsule increased adhesion. (mysciencework.com)
  • Cell yield after enzymatic digestion of the nasal cartilage was estimated to be 2,100 - 3,700 cells/mg of tissue [7] [12]. (wikipedia.org)
  • Nucleus pulposus cells (NPC) were obtained from 1-year-old bovine coccygeal discs by enzymatic digestion and were sorted for the angiopoietin-1 receptor Tie2. (biomedcentral.com)
  • Cells can be isolated from tissues for ex vivo culture in several ways. (wikipedia.org)
  • In recognition of this need, we recently established zebrafish embryos as a facile vertebrate in vivo system to monitor the effects of radiation protectors on normal tissues during development ( 4 ). (aacrjournals.org)
  • To compare the diagnosis of BCC in excised tissue ex vivo by high-definition optical coherence tomography (HD-OCT) with the findings of frozen-section histology in micrographic surgery. (saladgaffe.tk)
  • These results demonstrate that AP patterning is required for axial extension ex vivo and implies a crucial role for Nodal signaling at this intersection of tissue patterning and morphogenesis in vivo. (elifesciences.org)
  • Primary cells imitate the physiological state of other cells in vivo and generate more admissible data for researchers. (grandviewresearch.com)
  • Acute in vivo inhibition of testosterone by endotoxin parallels loss of steroidogenic acute regulatory (StAR) protein in Leydig cells. (faintpower.ml)
  • Implanted spheroids and cartilage tissue were co-cultured ex vivo for 12 weeks to allow regeneration processes to form new tissue within the cartilage defect. (biomedcentral.com)
  • The aim of this study was to determine in an animal model of bile duct ligation liver injury whether LT-β expression is induced and whether Kupffer cells are an intrahepatic source of LT-β. (edu.au)
  • Methods: Sprague-Dawley rats were divided into two groups: one group received a single dose of GdCl (a Kupffer cell-blocking agent, 10 mg/kg i.v.), whereas the other group received saline. (edu.au)
  • Liver tissue was obtained on days 1, 3, 5, and 8 for assessment of Kupffer cell numbers, early fibrogenic events and LT-β gene expression. (edu.au)
  • Kupffer cells were isolated using pronase/collagenase perfusion and centrifugal elutriation. (edu.au)
  • Pretreatment of bile duct-ligated animals with GdCl significantly reduced the number of Kupffer cells, delayed the rise in LT-β expression, but had no effect on fibrogenesis. (edu.au)
  • Recovery of the Kupffer cell population in these animals was accompanied by increased hepatic LT-β expression. (edu.au)
  • The LT-β ligand and receptor were expressed by isolated normal Kupffer cells. (edu.au)
  • Kupffer cells may be an intrahepatic source of LT-β. (edu.au)
  • Mutants which lack flagella ( flaA , flaB , and flbA ) or common cell surface antigen ( peb1A ) were constructed in strain 81-176 by natural transformation-mediated allelic exchange. (asm.org)
  • Immunohistochemistry (IHC) combines histological, immunological and biochemical techniques for the identification of specific tissue components by means of a specific antigen/antibody reaction tagged with a visible label. (creative-diagnostics.com)
  • The advantage of IHC over immunofluorescence techniques is the visible morphology of the tissue around the specific antigen by counterstaining, e.g., with hematoxylin (blue). (creative-diagnostics.com)
  • One of the main purposes of immunohistochemistry is to visualize the morphology and architecture of the tissue sample and its relationship with the target antigen. (creative-diagnostics.com)
  • The sample preparation steps are very important for a successful immunohistochemistry by maintaining the nature structure of tissue and activity of the target antigen. (creative-diagnostics.com)
  • Additionally, in an allogeneic cell transfer protocol, antigen presentation by AMs was Zbtb7a-dependent where AMs deficient in Zbtb7a failed to induce antibody and T cell responses. (sciencemag.org)
  • 1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. (exalpha.com)
  • Systemic immunisation of mice by intramuscular injection of plasmid DNA expressing HBV envelope proteins and HBV surface antigen (HBsAg) induces rapid, strong and sustained humoral and cell-mediated immune responses. (bmj.com)
  • MAb 6B3) which reacts specifically with a cell wall antigen found in all strains or isolates of Candida krusei was developed. (asm.org)
  • Cultures were maintained on a Sabouraud glucose agar (SGA) slant (bioMérieux) at 22°C, and blastoconidia were prepared by growing the cells on this medium for 48 h at 37°C. In some experiments, the influences of growth in different media and at different temperatures (22 and 37°C) on the surface expression of the antigen reacting with MAb 6B3 were investigated. (asm.org)
  • So the factors that you must consider can be roughly broken down into the different specimen formats that are available: fixation methods, processing tissue into paraffin wax, antigen retrieval, detection systems, controls, counterstains, specimen preparation for analysis and, finally, we'll pull everything together with a quick caption summary. (news-medical.net)
  • IHC, or immunohistochemistry, is immunological demonstration of an antigen on a tissue section. (news-medical.net)
  • Investigating what tissue has the antibody already been used to detect the antigen, what other tissues are expected to express the antigen? (news-medical.net)
  • Is the antigen expressed in normal tissues or only in certain disease states? (news-medical.net)
  • Similarly, look at which somatic laboratory cell lines the antibody has already been used on to detect the antigen. (news-medical.net)
  • What other somatic laboratory cell lines are expected to express the antigen? (news-medical.net)
  • Does the cell line need exposing to certain conditions grown on a certain support matrix, such as collagen, or need treating with a certain chemical in order to express the antigen? (news-medical.net)
  • neg, staining pattern of cells 'infected' with mock antigen. (nih.gov)
  • Recent studies suggest that activated pancreatic stellate cells (PSCs) play a central role in pancreatic fibrogenesis via increased synthesis of ECM proteins. (bmj.com)
  • It promotes cell lysis by activating a bacterial autolytic factor and is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces. (agscientific.com)
  • NC de-differentiation can be characterized by a gradual acquisition of a fibroblastic morphology, the expression of proteins associated with an undifferentiated mesenchymal cell phenotype (e.g., type I collagen and versican), and decreased expression of hyaline cartilage proteins (e.g., type II collagen and aggrecan) [9]. (wikipedia.org)
  • To better understand their functions in the regulation of intramuscular adipogenesis in goats, we cloned the three genes (AdipoQ, AdipoR1 and AdipoR2) encoding these proteins and detected their mRNA distribution in different tissues. (readbyqxmd.com)
  • They are matrix-producing cells with contractile properties, characterized by de novo expression of proteins shared with smooth muscle cells. (frontiersin.org)
  • Formalin fixes by penetrating the tissue and forming cross linkages between reactive amino groups in proteins. (creative-diagnostics.com)
  • The liver is a major source of circulating insulin-like growth factor I (IGF-I), and it also synthesizes several classes of IGF binding proteins (IGFBPs). (physiology.org)
  • Antibodies, which are initially of the IgM then IgG isotype (predominantly IgG2a), recognise several of the B cell epitopes present on the S, preS2, or preS1 domains of the envelope proteins. (bmj.com)
  • Each of these five proteins has a specific pattern of expression in embryonic and adult tissues with most tissues expressing at least one family member. (biomedcentral.com)
  • Four proteins with wall extension activity on grass cell walls were purified from maize ( Zea mays ) pollen by conventional column chromatography and high-performance liquid chromatography. (plantphysiol.org)
  • It is unknown whether the proteins encoded by these distantly related genes have expansin's characteristic activity, namely a rapid induction of cell wall extension and an increase in wall stress relaxation. (plantphysiol.org)
  • 1997 ) reported that the group 1 allergen from maize ( Zea mays ) pollen, called Zea m 1, has wall-loosening activity characteristic of expansins and proposed that these proteins loosen the cell walls of the stigma and style to aid pollen tube penetration. (plantphysiol.org)
  • Does cell confluency affect antigenic expression, for example, adhesion proteins exhibiting increased expression where cells touch? (news-medical.net)
  • Using functional annotation, we found that proteins belonging to pathways associated with regulation of the actin cytoskeleton, EGF/EGFR, TGF-β, MAPK signaling, integrin-mediated cell adhesion, and lipid metabolism were significantly enriched in the OA samples (p ≤10 −5 ). (biomedcentral.com)
  • For instance, Notch signaling is involved in fate determination and regulates satellite cell proliferation, and previous studies have shown that physical exercise increases the expression of Notch signaling pathway components-including ligands, Notch receptor, and downstream effectors-in myogenic cells [ 8 - 10 ]. (hindawi.com)
  • The propagation patterns of both cell types were similar, undergoing proliferation during the first 40 days and almost ceasing propagation over the remaining 20 days of the culture period. (ijbiotech.com)
  • At the blastocyst stage of pre-implantation mouse development, close contact of polar trophectoderm with the inner cell mass (ICM) promotes proliferation of undifferentiated diploid trophoblast. (biologists.org)
  • These findings suggest that the ectoplacental cavity may participate in maintaining proliferation throughout the developing chorionic ectoderm and, thus, in supporting its stem cell potential. (biologists.org)
  • CsA upregulated cell proliferation when it was added in the second culture period in the presence or in the absence of rIL-6, whereas rapamycin had no effect in these cases. (storysteel.cf)
  • We analyzed NR4A2 expression in fibrotic liver and stimulated HSCs compared with control group and studied the influence on cell proliferation, cell cycle, cell apoptosis and MAPK pathway after NR4A2 knockdown. (peerj.com)
  • In addition, NR4A2 silencing led to the promotion of cell proliferation, increase of cell percentage in S phase and reduced phosphorylation of ERK1/2, P38 and JNK in HSCs. (peerj.com)
  • NR4A members are widely distributed in cells mediating differentiation, proliferation and apoptosis and are involved in many diseases such as cancer, vascular sclerosis and metabolic syndrome. (peerj.com)
  • Following chronic liver injury, HSCs activate into α-SMA + contractile myofibroblast-like cells, which are characterized by the increased proliferation and migration, the unbalanced matrix degradation and the release of pro-inflammatory and pro-fibrogenic factors [ 2 ]. (mdpi.com)
  • CD147 is widely expressed on numerous cells in carcinoma leading to the invasion, proliferation and survival of cancer cells, which is an important marker of tumor and poor prognosis [ 8 , 9 , 10 ]. (mdpi.com)
  • The absence of PD-1 induced proliferation of effector T cells in the adenovirus-infected liver and resulted in rapid clearance of the virus. (rupress.org)
  • U87 cells induced an `activated' phenotype in HUVECs, including an increase in proliferation, migration and net-like formation. (newnetmediamarketing.com)
  • In this study, we uncovered a potent anti-osteoclastogenic effect of hyaluronan (HA), an ECM component present in bone marrow and soft connective tissues, in primary mouse and human osteoclast precursor cell cultures. (biologists.org)
  • Previously identified chondroinducible dermis cells were found to have multilineage differentiation capacity, and were subsequently termed dermis isolated adult stem cells (DIAS). (rice.edu)
  • Although it is well known that the number of satellite cells increases following physical exercise, functional alterations in satellite cells such as proliferative capacity and differentiation efficiency following exercise and their molecular mechanisms remain unclear. (hindawi.com)
  • Our analysis showed that functional overload induces the expression of MyoD in satellite cells and enhances the proliferative capacity and differentiation potential of these cells. (hindawi.com)
  • In addition, activated satellite cells express myogenic factor 5 (Myf5) and myogenic differentiation (MyoD) [ 7 ]. (hindawi.com)
  • There have been few studies examining functional alterations in satellite cells such as proliferative capacity and differentiation efficiency following exercise. (hindawi.com)
  • Leucocyte Typing V. White Cell Differentiation Antigens. (exalpha.com)
  • Similar to other chondrocytes from hyaline cartilage tissues in other locations in the human body, NC undergo a process of cell de-differentiation during monolayer culture. (wikipedia.org)
  • We also determined the role of AdipoQ in the adipogenic differentiation of goat skeletal muscle satellite cells (SMSCs). (readbyqxmd.com)
  • As such the use of EMD in combination with chondrogenically primed MSCs for periodontal bone tissue repair is unlikely to have negative effects on MSC differentiation. (frontiersin.org)
  • In brain tumours and metastases the antibody may be used for differentiation between glia derived tissue and tissues derived from other cell types. (nordicmubio.com)
  • S-100 helps in differentiation of Melanoma and Schwannoma from other soft tissue tumours. (nordicmubio.com)
  • Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. (patentsencyclopedia.com)
  • The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. (patentsencyclopedia.com)
  • The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. (patentsencyclopedia.com)
  • The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL). (biologists.org)
  • These cells are generated from monocyte/macrophage lineage of hematopoietic cells through a sophisticated differentiation program. (biologists.org)
  • At P3 the cells were subjected to immunophenotyping, multilineage differentiation, karyotype stability and colony forming efficiency. (exp-oncology.com.ua)
  • The cells were capable of directed osteogenic, adipogenic and chondrogenic differentiation. (exp-oncology.com.ua)
  • Down the differentiation tree, the interplay between lineage-specific networks of transcription factors and epigenetic modifications gradually decreases the multipotency ability of precursors and increases the compromise of cells within a particular lineage. (termsreign.ga)
  • 2006), aswell as merging it with various other enzymes (trypsin, pronase, hyaluronidase etc.) isn't uncommon (Jakob et al. (techuniq.com)
  • The results are discussed with respect to the origin of IgA plasma cells and the postulated bursal role of mammalian gut-associated lymphoid tissue. (jimmunol.org)
  • 1 . A therapeutic delivery system comprising an electrical pulse generator operably coupled with genetically engineered cells in a mammalian tissue, wherein said genetically engineered cells further comprise a target gene operably coupled to an electrically responsive promoter. (google.es)
  • In some embodiments, the tenocyte containing tissue may be isolated from any mammalian animal including, but not limited to a sheep, a cow, a pig or a human. (lens.org)
  • The cytotoxic effects of the extract on mammalian cells were investigated in three different cell line types by MTT assay. (saladgaffe.gq)
  • Barr "Mammalian Subtilisins: The Long-Sought Dibasic Processing Endoproteases", Cell, 66:1-3 (1991). (patentgenius.com)
  • One of many similarities to process the harvested tissues during the planning of the principal culture may be the utilize the enzyme type 2 collagenase (Hayman et al. (techuniq.com)
  • 2016). Even more particular problems of articular cartilage tissues engineering stay the high intake of cells and related costs, aswell as the planning of a perfect web host scaffold. (techuniq.com)
  • It was also determined that meniscus cells as a whole were more biomechanically similar to ligament cells than to articular chondrocytes, indicating that tissue properties may correlate with cellular mechanics. (rice.edu)
  • It was found that neither bovine nor leporine meniscus cells or articular chondrocytes caused activation of leporine immune cells, suggesting that they may serve as allogeneic or xenogeneic cell sources for meniscus engineering. (rice.edu)
  • Given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. (ijbiotech.com)
  • The majority of both articular and costal chondrocytes were observed to be organelle-rich round to oval cells embedded in lacuna-like cavities within the alginate beads. (ijbiotech.com)
  • Traditionally, cells for cartilage engineering are primarily obtained from articular knee cartilage (Arøen et al. (ijbiotech.com)
  • 1978). Since articular cartilage is a weight-bearing tissue providing smooth lubricated surfaces for joint movement, tissue removal impairs its function and imposes additional defects. (ijbiotech.com)
  • For the intended purpose, fibro cartilage and elastic cartilage are inappropriate because they differ from hyaline (articular) cartilage with respect to their structure, function, cell arrangement, and the presence or absence of perichondrium (Dehaven and Arnoczky, 1994). (ijbiotech.com)
  • Articular chondrocytes have typically been the cell type used for cartilage tissue engineering strategies for articular cartilage repair. (wikipedia.org)
  • The IVD consists of an inner nucleus pulposus (NP) surrounded by the annulus fibrosus (AF) tissue, and hyaline articular cartilage is located at the endplates between the IVD and the vertebral bodies. (biomedcentral.com)
  • Chondrocyte spheroids were generated from 14 donors, and implanted into a subchondral cartilage defect that was manually generated in human articular cartilage tissue. (biomedcentral.com)
  • However, articular hyaline cartilage has a low intrinsic repair capacity due to its lack of blood and lymphatic vessels as well its sparse cell population. (biomedcentral.com)
  • Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C, E or G (Art. (exalpha.com)
  • Unmasking with Pronase (Art. (exalpha.com)
  • In normal tissues it is found in glia and ependyma of the brain. (nordicmubio.com)
  • Peptides in a pronase digest of bovine serum albumin were required for streptolysin S formation by Streptococcus pyogenes besides maltose and a carrier (the oligonucleotide fraction obtained by treatment of Saccharomyces cerevisiae RNA with RNase A). The objective of this study was to evaluate whether the use of brief motivational interviewing (BMI) is effective in improving internal motivation for oral hygiene behaviour. (saladgaffe.tk)
  • 1992) Survival and integration of bovine chromaffin cells transplanted into rat central nervous system with exogenous trophic factors. (forexicreg.com)
  • However, the role of these cells in ECM protein degradation has not been fully elucidated. (bmj.com)
  • The specific epitope is expressed on the surface of fungal cells and appears to reside on a protein moiety. (asm.org)
  • Wall extension activity of Zea m 1d was biphasic with respect to protein concentration, had a broad pH optimum between 5 and 6, required more than 50 μg mL -1 for high activity, and led to cell wall breakage after only approximately 10% extension. (plantphysiol.org)
  • Cells of P. luminescens labeled with green fluorescent protein preferentially colonized a region of the DJ intestine immediately behind the basal bulb, extending for various distances toward the anus. (asm.org)
  • Brief hairpin RNA (shRNA) targeting sign transducer and activator of transcription 3 (STAT3) potentiate the radiosensitivity of human being laryngeal squamous carcinoma cells by regulating downstream signaling protein in the STAT3 pathway. (healthappsopenday.com)
  • In the repair tissue, aggrecan protein was expressed and extra-cellular matrix cartilage fibers were present, both indicative for a cartilage hyaline-like character of the repair tissue. (biomedcentral.com)
  • A positive correlation was found between high level of aggrecan protein expression in spheroids before implantation and a higher regeneration potential after implantation, reflected by more newly formed repair tissue. (biomedcentral.com)
  • This demonstrated that aggrecan protein expression levels in spheroids before implantation can potentially be used as surrogate potency assay for the cartilage cell transplant to predict its regenerative capacity after implantation in human patients. (biomedcentral.com)
  • Two post-implantation derivatives of the polar trophectoderm - early-streak extra-embryonic ectoderm and late-streak chorionic ectoderm - were microdissected into fractions along their proximodistal axis and thoroughly dissociated for trophoblast stem cell culture. (biologists.org)
  • Results indicated that cells with trophoblast stem cell potential were distributed throughout the extra-embryonic/chorionic ectoderm, an observation that is probably attributable to non-coherent growth patterns exhibited by single extra-embryonic ectoderm cells at the onset of gastrulation. (biologists.org)
  • Furthermore, the frequency of cells with trophoblast stem cell potential increased steadily in extra-embryonic/chorionic ectoderm until the first somite pairs formed, decreasing thereafter in a manner independent of proximity to the allantois. (biologists.org)
  • In the mouse, the first cells to differentiate from the fertilised egg are those of the extra-embryonic trophoblast lineage, whose ultimate role is to facilitate maternal/foetal exchange in the mature chorioallantoic placenta ( Cross, 2000 ). (biologists.org)
  • The trophectoderm gives rise to the ectoplacental cone, extra-embryonic ectoderm and the trophoblast giant cells. (biologists.org)
  • The epiblast gives rise to the three germ layers (ectoderm, mesoderm and endoderm) that contain the progenitors of all the tissues of the fetus, and to the extra-embryonic mesoderm of the visceral yolk sac, the allantois and the amnion. (biologists.org)
  • The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine. (patentsencyclopedia.com)
  • The development of cell biology and regenerative medicine methods open up new opportunities for the treatment of a number of pathological conditions, the study of their pathogenesis and the possibilities for correction. (exp-oncology.com.ua)
  • For this reason, it is necessary to use tissue-specific MSC for development of cell-based regenerative medicine approaches. (exp-oncology.com.ua)
  • Interest for MSC using in regenerative medicine related not only of their structural integration in damaged or diseased tissues and organs, but also for their trophic effect. (exp-oncology.com.ua)
  • Gene therapy, regenerative medicine, and cancer research exhibit lucrative growth opportunities and this trend is fuelled by rising geriatric population, increase in prevalence of chronic illnesses, and highly advantageous and upcoming uses of stem cell therapies. (grandviewresearch.com)
  • Developmental programmes within multicellular organisms originate from a single cell ( i.e. a fertilised oocyte) that proliferates into numerous cells ultimately differentiating to make up specialised tissues and organs. (elifesciences.org)
  • Optimisation of isolation yields, abundant NVP-BGJ398 cell signaling cell sources and efficient culturing procedures that lead to preparation of desired, reproducible and relatively affordable cell cultures or/and material-cell constructs with good durability are therefore highly rated novelties in recent research (Dehne et al. (techuniq.com)
  • To be successful tissue replacements, tissue engineered meniscus constructs must not elicit an immune response and must have sufficient mechanical properties to survive when implanted. (rice.edu)
  • DIAS cells were also able to be expanded in monolayer without losing chondroinductive capacity, and were able to create constructs with cartilaginous properties which could be varied with growth factor application. (rice.edu)
  • Collectively, these data suggest that the costal chondrocytes could provide a beneficial and more accessible source of chondrocytes for three dimensional (3D) cartilage constructs. (ijbiotech.com)
  • Pre-clinical investigations have been undertaken using various animal models to provide proof-of-principle of the clinical potential of NC-based tissue engineered constructs for cartilage reconstruction. (wikipedia.org)
  • making them an attractive cell source for bone tissue engineered constructs. (frontiersin.org)
  • Bone tissue constructs modeled after the process of endochondral ossification (EO) may result in more promising outcomes as the tissue would be better suited to survive the initial avascular implantation site. (frontiersin.org)
  • Methods that have been used to examine oxygen gradients in tissue or three-dimensional constructs include computational modeling, 1 photo-acoustic microscopy, 2 electron paramagnetic resonance combined with magnetic resonance imaging, 3 , 4 invasive probes, and non-invasive luminescence. (spiedigitallibrary.org)
  • 5 , 6 Modeling approaches rely heavily on experimental data using the Michaelis-Menten equation to describe the maximum oxygen consumption rate of cells 7 combined with Fick's 2nd law governing diffusion to estimate macro-gradients in tissues or constructs. (spiedigitallibrary.org)
  • Skeletal muscle represents a plentiful and accessible source of adult stem cells. (hindawi.com)
  • Fast-spiking (FS) interneurons are important elements of neocortical circuitry that constitute the primary source of synaptic inhibition in adult cortex and impart temporal organization on ongoing cortical activity. (jneurosci.org)
  • Although embryonic transcriptional events govern the initial steps of cell-type specification in most cortical interneurons, including FS cells, the electrophysiological properties that distinguish adult cortical cell types emerge relatively late in postnatal development, and the transcriptional events that drive this maturational process are not known. (jneurosci.org)
  • Using our microarray screen as a guide, we discovered that upregulation of two-pore K + leak channels between P10 and P25 contributes to one of the major differences between the intrinsic membrane properties of immature and adult FS cells and found a number of other candidate genes that likely confer cell-type specificity on mature FS cells. (jneurosci.org)
  • The lesions consisted of 7 with low-grade mucosa-associated lymphoid tissue (MALT), 5 with gastric involvement by adult T-cell leukemia/lymphoma (ATLL), 4 with diffuse large B-cell lymphoma (DLBCL), and 1 with peripheral T-cell lymphoma. (biomedcentral.com)
  • However, CMV proctitis was subsequently diagnosed by immunohistochemical staining of biopsied rectal tissues using an anti-CMV antibody, although 3 biopsies of the rectum were necessary for the final and correct diagnosis. (alliedacademies.org)
  • Following transient expression in cos cells, all of the constructions were capable of producing humanlike antibody. (iospress.com)
  • 1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. (exalpha.com)
  • Transfer experiments involving fractionated HBsAg primed spleen cells obtained from DNA immunised mice into Tg mice showed that both CD4+ and CD8+ T cells were able to control transgene expression even in the absence of antibody production. (bmj.com)
  • SARS-CoV was plaque purified from a human isolate (Tor 3) on Vero E6 cells by using the plaquing method we describe in the SARS-CoV antibody detection section. (cdc.gov)
  • 2012). NVP-BGJ398 cell signaling The state-of-the-art idea of cartilage tissues advancement combines the usage of biodegradable and biocompatible carrier components, the use of development factors, the usage of different cell types (stem or currently differentiated) and various methods to simulate the indigenous mechanical excitement (Gardner et Rabbit Polyclonal to IKK-alpha/beta (phospho-Ser176/177) al. (techuniq.com)
  • 2012). Several methods for chondrocyte isolation from various tissue parts and organisms were introduced over the last decades (Hu et al. (techuniq.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • It will be appreciated that the tenocytes used in the methods and devices of the invention as described herein can be isolated from any tenocyte-containing tissue. (lens.org)
  • The invention is also concerned with methods of forming pooled preparations of cells using single-cryopreserved compositions of cells. (patentsencyclopedia.com)
  • Also currently available methods by which human red blood cells are labeled with Tc-99m for clinical use and their pharmacokinetics were discussed. (isorbe.com)
  • Following washing of the cultures, the quantity of radiolabel associated with the cells was measured as described in the Materials and methods. (nih.gov)
  • Virus was added at 10 tissue culture infection dose 50% (TCID 50 )/cell, as described in the Materials and methods. (nih.gov)
  • A method for producing Influenza and other viruses and vaccines derived therefrom utilizes serum-free cultured vertebrate cells or vertebrate biomass aggregates to both eliminate the necessity to use costly methods requiring whole chicken embryos and, optionally, to provide proteases suitable for the activation of a wide variety of viruses. (patentgenius.com)
  • Part of the challenge when choosing a fixative is the fact that the amounts of antigens or molecular targets within tissue specimens are finite. (creative-diagnostics.com)
  • Pancreatic stellate cells have the capacity to synthesise a number of matrix metalloproteinases, including MMP2, MMP9, and MMP13 and their inhibitors TIMP1 and TIMP2. (bmj.com)
  • Hepatic stellate cells (HSCs), known as perisinusoidal cells containing auto-fluorescent vitamin A, are the major although not exclusive source of myofibroblasts in the injured liver. (frontiersin.org)
  • Activated hepatic stellate cells (HSCs) release pro-inflammatory and pro-fibrogenic factors. (mdpi.com)
  • The intervertebral disc (IVD) has limited self-healing potential and disc repair strategies require an appropriate cell source such as progenitor cells that could regenerate the damaged cells and tissues. (biomedcentral.com)
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to examine gene expression of MMP9 and the tissue inhibitors of metalloproteinases TIMP1 and TIMP2. (bmj.com)
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • Recent studies (2) in our laboratory demonstrated that aberrant promoter methylation of the p16 tumor suppressor gene, which plays a key role in cell cycle regulation, is an early and very frequent event in SCC of the lung. (aacrjournals.org)
  • The highly specialized intrinsic membrane and firing properties that allow cortical FS interneurons to perform these functions are attributable to equally specialized gene expression, which is ultimately coordinated by cell-type-specific transcriptional regulation. (jneurosci.org)
  • We characterized both whole transcriptome expression and the electrophysiology of these cells at a range of postnatal ages and found that FS cells exhibit profound developmental regulation of both gene expression and physiology over the first 4 postnatal weeks. (jneurosci.org)
  • Comparing the temporal patterns of gene regulation revealed by our microarray with maturational changes in the intrinsic and synaptic properties of FS cells allowed us to correlate the expression of subsets of genes with the onset of multiple FS cell phenotypes. (jneurosci.org)
  • Gene expression can be stably altered through epigenetic changes which not only allow cells and organisms to quickly respond to changing environmental stimuli but also confer the ability of the cell to "memorize" these encounters once the stimulus is removed. (jove.com)
  • Changes in gene expression are often controlled by mobile signals that translate positional information into cell type-specific transcriptional outputs ( Hironaka and Morishita, 2012 ). (elifesciences.org)
  • Gene expression analysis and intratumoral microvessel density (MVD) Tumor tissues were fixed in 4% paraformalde-hyde and embedded in paraffin then 4-mm sections were cut and prepared. (healthappsopenday.com)
  • title = Rearrangements of the red-cell membrane glycophorin C (sialoglycoprotein beta) gene. (wikidoc.org)
  • Analysis of qRT-PCR data from virus and mock infected cells using NormFinder and BestKeeper software programmes found that 18S rRNA was the most stable gene in HBECs, PTECs and avian lung cells. (biomedcentral.com)
  • Based on the presented data from cell culture models (HBECs, PTECs, chicken and duck lung cells) infected with a range of influenza viruses, we found that 18S rRNA is the most stable reference gene for normalising qRT-PCR data. (biomedcentral.com)
  • An ideal reference/ housekeeping gene should be stably expressed across samples from different tissues, developmental stages, and experimental conditions. (biomedcentral.com)
  • Virus infection of cells leads to a general inhibition of cellular macromolecular synthesis that is referred to as shut-off [ 11 ] and causes changes in global gene expression. (biomedcentral.com)
  • During vertebrate gastrulation, convergence and extension (C and E) of the primary anteroposterior (AP) embryonic axis is driven by polarized mediolateral (ML) cell intercalations and is influenced by AP axial patterning. (elifesciences.org)
  • Tissue degradation begins at the time of sample removal. (creative-diagnostics.com)
  • Treatments with IAA but not BA protected ETT-FLAG from pronase-induced degradation consistent with direct, specific interaction between IAA and ETT in planta. (elifesciences.org)
  • COL1 expression has confirmed the tendency of the isolated cells dedifferentiation into a fibroblast-like phenotype already in the second passage, which confirms previous findings that higher passages should be used with care in cartilage tissue engineering. (techuniq.com)
  • The present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. (ijbiotech.com)
  • Additionally NC have been recently shown to exhibit features of self-renewal capability, being able to form cartilage tissue following serial cloning possibly due to their neuro-ectodermal origin[30]. (wikipedia.org)
  • In the biopsied rectal tissues, large mesenchymal cells with large nuclei were found near the erosion, but no distinct intranuclear inclusions were visible ( Fig. 1b ). (alliedacademies.org)
  • These large mesenchymal cells were obvious in the first and the second biopsied tissue samples. (alliedacademies.org)
  • Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. (frontiersin.org)
  • MSCs were chondrogenically differentiated in 2.0 × 10 5 cell pellets in medium supplemented with TGFβ3 in the absence or presence of 1, 10, or 100 μg/mL EMD. (frontiersin.org)
  • The IGFBPs may act to control transport of IGF-I from the circulation to specific tissues, to provide for cell- and tissue-specific localization of IGF-I, and to modulate the interaction between IGF-I and its receptor ( 15 ). (physiology.org)
  • The accumulation of drugs and/or their metabolites in specific tissues often leads to toxic events. (aspetjournals.org)
  • Fluvin binds to the fungal p450 enzymes and stops the cells making ergosterol, the main component of the cell wall. (faintpower.ml)
  • Among a number of CD68 antibodies PG-M1 is one of the most specific ones, since it stains neither myeloid precursor cells, nor granulocytes or dendritic cells. (exalpha.com)
  • Although organic cation transport mechanisms in the kidney and liver have been well characterized, experimental assessment of the mechanisms used in other tissues, such as the choroid plexus, is limited by the small size and anatomic inaccessibility of the transporting epithelia. (aspetjournals.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • To receive news and publication updates for Stem Cells International, enter your email address in the box below. (hindawi.com)
  • Skeletal-muscle-specific stem cells, termed satellite cells, contribute to the postnatal maintenance, growth, repair, and regeneration of skeletal muscle [ 1 ]. (hindawi.com)
  • This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. (patentsencyclopedia.com)
  • 21. A method for producing a population of cells comprising cells that express β-tubullin III comprising:b) culturing primate pluripotent stem cells in a medium containing a neurotrophin thereby producing a population of cells expressing β-tubullin III. (patentsencyclopedia.com)
  • 22. The method of claim 21, wherein the primate pluripotent stem cells are plated and cultured on a solid surface so that they differentiate without forming embryoid bodies. (patentsencyclopedia.com)
  • For this reasons the MSC are the most commonly used stem cells in current clinical applications. (exp-oncology.com.ua)
  • The majority of the day dealt with stem cell imaging and current research of different therapeutic uses of stem cells. (isorbe.com)
  • Several interesting and well-conducted presentations demonstrated the wonderful research opportunities arising/being conducted with stem cells and the future therapeutic impact that they can have. (isorbe.com)
  • Content of dolichol and retinol in isolated rat non-parenchymal liver cells( Ref. 0106 ). (lipidbank.jp)
  • Portal myofibroblasts (PMFs) have been defined as liver myofibroblasts derived from cells that are distinct from HSCs and located in the portal tract. (frontiersin.org)
  • Therapeutic delivery of this α v β 1 inhibitor attenuated bleomycin-induced pulmonary fibrosis and carbon tetrachloride-induced liver fibrosis, suggesting that drugs based on this lead compound could be broadly useful for treatment of diseases characterized by excessive tissue fibrosis. (sciencemag.org)
  • NR4A2 expression was significantly lower in fibrotic liver tissues and PDGF BB or TGF- β stimulated HSCs compared with control group. (peerj.com)
  • HSCs, also known as Ito cells or perisinusoidal cells, are pericytes found in the perisinusoidal space of the liver. (peerj.com)
  • Two IGF-I mRNA subtypes with different 5′ ends (class 1 and class 2) were detected in all isolated liver cell preparations. (physiology.org)
  • GH receptor was expressed in all cell preparations, consistent with GH regulation of IGF-I and IGFBP synthesis in multiple liver cell types. (physiology.org)
  • Cell-specific expression of distinct IGFBPs in the liver provides the potential for cell-specific regulation of hepatic and endocrine actions of IGF-I. (physiology.org)
  • insulin-like growth factor I (IGF-I) is synthesized in most if not all tissues, but the liver appears to be a major source of circulating IGF-I ( 17 , 19 ). (physiology.org)
  • IGF-I mRNA abundance is 40- to 100-fold higher in liver than in nonhepatic tissues ( 20 ). (physiology.org)
  • The liver has not been traditionally considered a major target tissue for IGF-I actions because hepatocyte cell membranes contain few type 1 IGF receptors and type 1 IGF receptor mRNA is not readily detected in poly(A) + RNA prepared from whole rat liver ( 23 , 26 ). (physiology.org)
  • Because hepatocytes represent the majority of cells within the liver, these observations do not exclude the possibility that type 1 IGF receptor is expressed in less abundant liver cell types. (physiology.org)
  • The liver is a known source of IGFBP-1 to -4 ( 1 , 12 , 30 , 33 ). (physiology.org)
  • We purified LMs from liver explants and included treatment with a T cell immunotoxin to reduce T cells to 1% or less. (jci.org)
  • The latter present the ideal source for a simple and relatively cheap isolation of chondrocytes as was confirmed in our study. (techuniq.com)
  • Among these, cartilage repair using tissue engineering should in theory be more straightforward given its avascular nature and single cell type (chondrocytes) construction. (ijbiotech.com)
  • Nasal chondrocytes (NC) are present in the hyaline cartilage of the nasal septum, and in fact are the only cell type within the tissue. (wikipedia.org)
  • Using this technique, quantification of oxygen concentrations around isolated viable chondrocytes, seeded in three-dimensional agarose gel, revealed a subpopulation of cells that exhibited significant spatial oxygen gradients such that oxygen concentration reduced with increasing proximity to the cell. (spiedigitallibrary.org)
  • Zebrafish embryonic cells attach well to glass coated with laminin or to plastic. (zfin.org)
  • Because, at these stages, the early embryonic cells are not yet restricted in their lineage potency to contribute to the inner cell mass or the trophectoderm, they are equally capable of contributing to both lineages. (biologists.org)
  • It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. (exalpha.com)
  • 1,2 The mechanisms by which macrophage apoE is antiatherogenic may include stimulating the removal of excess cholesterol from macrophage foam cells, 3 as well as anti-inflammatory, 4 antiproliferative, 5 and immunomodulatory properties. (ahajournals.org)
  • In response to muscle injury or exercise, satellite cells are activated and proliferate and differentiate into mature fibers [ 3 ]. (hindawi.com)
  • 15. A method for producing a population of cells comprising cells that express tyrosine hydroxylase comprising:a) plating and culturing undifferentiated primate pluripotent stem (pPS) cells on a solid surface so that they differentiate without forming embryoid bodies;b) culturing the plated cells in a medium containing noggin and/or follistatin thereby producing a population of cells expressing tyrosine hydroxylase. (patentsencyclopedia.com)
  • When naive T cells differentiate into effector cells, they change their activation requirement. (rupress.org)
  • In addition, there are components available in the market that can differentiate between other cell types. (grandviewresearch.com)
  • Pluripotent embryonic stem (ES) cells derived from the inner cell mass of the blastocyst ( Fig. 1 ), can differentiate into all tissue types in chimeras, including the germline ( Robertson, 1986 ). (biologists.org)
  • Tissue biopsies were repeated 3 times in total during the patient's 4-month admission, because a definite diagnosis could not be made. (alliedacademies.org)
  • The explant method is primarily used for skin biopsies and small quantities of tissues. (grandviewresearch.com)
  • Here, we found that functional overload, which is widely used to model resistance exercise, causes skeletal muscle hypertrophy and converts satellite cells from quiescent state to activated state. (hindawi.com)
  • These cells are located between the basal lamina and plasma membrane of skeletal muscle fibers in which they represent 2.5%-6% of all nuclei and remain in a quiescent state under normal physiological conditions [ 2 ]. (hindawi.com)
  • Satellite cells can be mitotically quiescent or in an activated proliferative state during skeletal muscle turnover. (hindawi.com)
  • Osteoclasts are cells specialized for the function of bone catabolism and play, together with osteoblasts, important roles in skeletal development, bone remodeling and fracture healing. (biologists.org)
  • and ACAN offers been shown how the proposed protocol potential clients to isolation of cells with a NVP-BGJ398 cell signaling high chondrogenic potential, possibly even specific phenotype preservation up to the second passage. (techuniq.com)
  • These findings illustrate that known regional variations in meniscus cell phenotype and biochemical composition are also evident in cellular mechanics, and phenotypic responses of these cells to isolation are varied and distinct. (rice.edu)
  • In order to improve the preservation of the Tie2+ phenotype of NPPC in monolayer cultures, we tested a selection of growth factors known to have stimulating effects, cocultured NPPC with IVD tissue, and exposed them to hypoxic conditions (2 % O 2 ). (biomedcentral.com)
  • However, fixation itself introduces artifacts and the ideal fixative would also maintain the structure of all of the epitopes in the tissues. (creative-diagnostics.com)
  • Increased tumour cell TSP-1 expression was related to increased microvessel density. (biomedcentral.com)
  • The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. (wikipedia.org)
  • With the exception of some derived from tumors, most primary cell cultures have limited lifespan. (wikipedia.org)
  • Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures. (vwr.com)
  • This regurgitation reaction was induced by a low-molecular-weight, heat- and protease-stable, anionic component present in arthropod hemolymph and in supernatants from insect cell cultures. (asm.org)
  • We propose that the nucleus pulposus represents a niche of precursor cells for regeneration of the IVD. (biomedcentral.com)
  • Although αvβ3 is a major receptor that mediates EC attachment to FBG, cell adhesion to desAB fibrin (fibrin lacking FPA and FPB) is partially resistant to inhibition by RGD-containing peptides and an MoAb against integrin receptor αvβ3 (7E3). (ahajournals.org)
  • however, unlike most integrin receptor-ligand interactions, its binding to β15-42 is neither inhibited by RGDS cell binding domain peptides nor requires divalent cations. (ahajournals.org)
  • Together, these findings suggest that this amino sugar may be a component of the receptor on both animal cell surfaces and that the fimbriae may be the adhesions. (mysciencework.com)
  • To analyze the incidence and profile of mutations in epidermal growth factor receptor (EGFR) in Chinese patients with non-small cell lung cancer (NSCLC). (faintpower.tk)
  • However, whether NK cells in general and NKp46 (a major killer receptor expressed by NK cells) in particular, play a protective or destructive role in this disease is unknown. (pubmedcentralcanada.ca)
  • NR4A2 is a nuclear receptor belonging to the NR4A subfamily and vital in regulating cell growth, metabolism, inflammation and other biological functions. (peerj.com)
  • We also measured class 1 and class 2 IGF-I mRNAs and GH receptor mRNA expression in these cell populations to determine whether these cells represent potential target cells for IGF-I. (physiology.org)
  • Unlike naive T cells, effector T cells can be activated by either T cell receptor signal or costimulatory signal alone and therefore the absence of costimulatory molecules on tissue cells cannot explain the tolerance mechanism at the effector phase. (rupress.org)
  • Here we report that PD-L1, the ligand for the immunoinhibitory receptor PD-1, was expressed on vascular endothelium in peripheral tissues. (rupress.org)
  • Thus, cultured endometrial stromal cells meet minimal ISCT criteria for MSC. (exp-oncology.com.ua)
  • Mesenchymal stem/stromal cells (MSC), the major cell type for cell therapy, have been used in the clinic for approximately 15 years [1]. (exp-oncology.com.ua)
  • So, the study of secretion profile of interleukins (IL), chemokines and growth factors of endometrial mesenchymal stromal cells (eMSC) is important task. (exp-oncology.com.ua)
  • Specifically, certain triterpenoids (CDDO and derivatives thereof) have been shown to selectively protect normal mouse tissues against the deleterious effects of ionizing radiation ( 11 ). (aacrjournals.org)
  • Adhesion of type A Pasteurella mulocida to rabbit pharyngeal cells and its possible role in rabbit respiratory tract infections. (mysciencework.com)
  • The collagen compositions prepared accordingly can be used as medical adhesives for bonding soft tissues or be made in to a sealant film for a variety of medical uses such as wound closures and tendon wraps for preventing adhesion formation following surgery. (google.com.au)
  • To determine: (i) whether PSCs secrete matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) and, if so (ii) whether MMP and TIMP secretion by PSCs is altered in response to known PSC activating factors such as tumour necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1), interleukin 6 (IL-6), ethanol, and acetaldehyde. (bmj.com)
  • By using subcutaneous chambers inoculated with F. nucleatum we demonstrate that immune cells, including NK cells, rapidly accumulate in the chambers and that this leads to a fast and transient, NCR1-dependant TNF-α secretion. (pubmedcentralcanada.ca)
  • Western ligand and immunoblot analyses were used to determine IGFBP secretion by isolated cells. (physiology.org)
  • The scaffolds are useful for regenerating missing interface tissue between two adjacent tissues, or regeneration and integration of two adjacent tissues directly. (freepatentsonline.com)
  • This requires the development of a potency assay, which assesses a characteristic of the cell transplant before implantation that can predict its cartilage regeneration capacity after implantation. (biomedcentral.com)
  • The amount of formed repair tissue was used as a read-out for regeneration capacity and was correlated with the spheroid characteristics determined before implantation. (biomedcentral.com)
  • The histological classification may range from a low-grade type of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) to a high-grade type of diffuse large B-cell lymphoma (DLBCL) [ 1 , 2 ]. (biomedcentral.com)
  • So the generation of new vascular cells to restore organ function following a myocardial infarction is of high research interest. (newnetmediamarketing.com)
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • In addition, several appropriate techniques are subsequently required to seed the carrier scaffold with cells to construct a 3D tissue implant that is to be transplanted in the defective site. (ijbiotech.com)
  • Immunohistochemical examination was subsequently performed using a streptavidin biotin complex method, because at the time of the third biopsy, infection with CMV was suspected, despite no typical nuclear inclusions being detected in these tissues. (alliedacademies.org)
  • It initially penetrates and fixes tissues more readily than formalin (although penetration slows down subsequently), and is often recommended for nucleic acid work. (creative-diagnostics.com)