Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Laboratories, Dental: Facilities for the performance of services related to dental treatment but not done directly in the patient's mouth.Dental Technicians: Individuals responsible for fabrication of dental appliances.Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Technology, Dental: The field of dentistry involved in procedures for designing and constructing dental appliances. It includes also the application of any technology to the field of dentistry.Oral Medicine: A branch of dentistry dealing with diseases of the oral and paraoral structures and the oral management of systemic diseases. (Hall, What is Oral Medicine, Anyway? Clinical Update: National Naval Dental Center, March 1991, p7-8)Cellophane: A generic name for film produced from wood pulp by the viscose process. It is a thin, transparent sheeting of regenerated cellulose, moisture-proof and sometimes dyed, and used chiefly as food wrapping or as bags for dialysis. (Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Islets of Langerhans: Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.LIM-Homeodomain Proteins: A subclass of LIM domain proteins that include an additional centrally-located homeodomain region that binds AT-rich sites on DNA. Many LIM-homeodomain proteins play a role as transcriptional regulators that direct cell fate.Adenoma, Islet Cell: A benign tumor of the pancreatic ISLET CELLS. Usually it involves the INSULIN-producing PANCREATIC BETA CELLS, as in INSULINOMA, resulting in HYPERINSULINISM.Islets of Langerhans Transplantation: The transference of pancreatic islets within an individual, between individuals of the same species, or between individuals of different species.Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Nail-Patella Syndrome: A syndrome of multiple abnormalities characterized by the absence or hypoplasia of the PATELLA and congenital nail dystrophy. It is a genetically determined autosomal dominant trait.Journalism, Medical: The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.Psychiatry: The medical science that deals with the origin, diagnosis, prevention, and treatment of mental disorders.Library Services: Services offered to the library user. They include reference and circulation.Cardiology: The study of the heart, its physiology, and its functions.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Neurosciences: The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Arthritis, Rheumatoid: A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.Joints: Also known as articulations, these are points of connection between the ends of certain separate bones, or where the borders of other bones are juxtaposed.Mice, Inbred C57BLTetrazolium Salts: Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455)ArchivesFormazans: Colored azo compounds formed by the reduction of tetrazolium salts. Employing this reaction, oxidoreductase activity can be determined quantitatively in tissue sections by allowing the enzymes to act on their specific substrates in the presence of tetrazolium salts.Leydig Cells: Steroid-producing cells in the interstitial tissue of the TESTIS. They are under the regulation of PITUITARY HORMONES; LUTEINIZING HORMONE; or interstitial cell-stimulating hormone. TESTOSTERONE is the major androgen (ANDROGENS) produced.Gonadal Dysgenesis: A number of syndromes with defective gonadal developments such as streak GONADS and dysgenetic testes or ovaries. The spectrum of gonadal and sexual abnormalities is reflected in their varied sex chromosome (SEX CHROMOSOMES) constitution as shown by the karyotypes of 45,X monosomy (TURNER SYNDROME); 46,XX (GONADAL DYSGENESIS, 46XX); 46,XY (GONADAL DYSGENESIS, 46,XY); and sex chromosome MOSAICISM; (GONADAL DYSGENESIS, MIXED). Their phenotypes range from female, through ambiguous, to male. This concept includes gonadal agenesis.Leydig Cell Tumor: Gonadal interstitial or stromal cell neoplasm composed of only LEYDIG CELLS. These tumors may produce one or more of the steroid hormones such as ANDROGENS; ESTROGENS; and CORTICOSTEROIDS. Clinical symptoms include testicular swelling, GYNECOMASTIA, sexual precocity in children, or virilization (VIRILISM) in females.Testicular Neoplasms: Tumors or cancer of the TESTIS. Germ cell tumors (GERMINOMA) of the testis constitute 95% of all testicular neoplasms.Cryptorchidism: A developmental defect in which a TESTIS or both TESTES failed to descend from high in the ABDOMEN to the bottom of the SCROTUM. Testicular descent is essential to normal SPERMATOGENESIS which requires temperature lower than the BODY TEMPERATURE. Cryptorchidism can be subclassified by the location of the maldescended testis.Hypospadias: A birth defect due to malformation of the URETHRA in which the urethral opening is below its normal location. In the male, the malformed urethra generally opens on the ventral surface of the PENIS or on the PERINEUM. In the female, the malformed urethral opening is in the VAGINA.Neoplasms, Germ Cell and Embryonal: Neoplasms composed of primordial GERM CELLS of embryonic GONADS or of elements of the germ layers of the EMBRYO, MAMMALIAN. The concept does not refer to neoplasms located in the gonads or present in an embryo or FETUS.Drug Discovery: The process of finding chemicals for potential therapeutic use.Immune System: The body's defense mechanism against foreign organisms or substances and deviant native cells. It includes the humoral immune response and the cell-mediated response and consists of a complex of interrelated cellular, molecular, and genetic components.Antigen-Presenting Cells: A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.Immunotherapy: Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Biphasic Insulins: An insulin preparation that is designed to provide immediate and long term glycemic control in a single dosage. Biphasic insulin typically contains a mixture of REGULAR INSULIN or SHORT-ACTING INSULIN combined with a LONG-ACTING INSULIN.

Emerging targets: molecular mechanisms of cell contact-mediated growth control. (1/45748)

Contact inhibition of cell proliferation evokes a unique cellular program of growth arrest compared with stress, age, or other physical constraints. The last decade of research on genes activated by cell-cell contact has uncovered features of transmembrane signaling, cytoskeletal reorganization, and transcriptional control that initiate and maintain a quiescent phenotype. This review will focus on mechanisms controlling contact inhibition of cell proliferation, highlighting specific gene expression responses that are activated by cell-cell contact. Although a temporal framework for imposition of these mechanisms has not yet been well described, contact inhibition of cell proliferation clearly requires their coordinated function. Novel targets for intervention in proliferative disorders are emerging from these studies.  (+info)

Cell cycle dysregulation in oral cancer. (2/45748)

The dysregulation of the molecular events governing cell cycle control is emerging as a central theme of oral carcinogenesis. Regulatory pathways responding to extracellular signaling or intracellular stress and DNA damage converge on the cell cycle apparatus. Abrogation of mitogenic and anti-mitogenic response regulatory proteins, such as the retinoblastoma tumor suppressor protein (pRB), cyclin D1, cyclin-dependent kinase (CDK) 6, and CDK inhibitors (p21(WAF1/CIP1), p27(KIP1), and p16(INK4a)), occur frequently in human oral cancers. Cellular responses to metabolic stress or genomic damage through p53 and related pathways that block cell cycle progression are also altered during oral carcinogenesis. In addition, new pathways and cell cycle regulatory proteins, such as p12(DOC-1), are being discovered. The multistep process of oral carcinogenesis likely involves functional alteration of cell cycle regulatory members combined with escape from cellular senescence and apoptotic signaling pathways. Detailing the molecular alterations and understanding the functional consequences of the dysregulation of the cell cycle apparatus in the malignant oral keratinocyte will uncover novel diagnostic and therapeutic approaches.  (+info)

Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. (3/45748)

BACKGROUND: The Hedgehog (Hh) signaling pathway is vital to animal development as it mediates the differentiation of multiple cell types during embryogenesis. In adults, Hh signaling can be activated to facilitate tissue maintenance and repair. Moreover, stimulation of the Hh pathway has shown therapeutic efficacy in models of neuropathy. The underlying mechanisms of Hh signal transduction remain obscure, however: little is known about the communication between the pathway suppressor Patched (Ptc), a multipass transmembrane protein that directly binds Hh, and the pathway activator Smoothened (Smo), a protein that is related to G-protein-coupled receptors and is capable of constitutive activation in the absence of Ptc. RESULTS: We have identified and characterized a synthetic non-peptidyl small molecule, Hh-Ag, that acts as an agonist of the Hh pathway. This Hh agonist promotes cell-type-specific proliferation and concentration-dependent differentiation in vitro, while in utero it rescues aspects of the Hh-signaling defect in Sonic hedgehog-null, but not Smo-null, mouse embryos. Biochemical studies with Hh-Ag, the Hh-signaling antagonist cyclopamine, and a novel Hh-signaling inhibitor Cur61414, reveal that the action of all these compounds is independent of Hh-protein ligand and of the Hh receptor Ptc, as each binds directly to Smo. CONCLUSIONS: Smo can have its activity modulated directly by synthetic small molecules. These studies raise the possibility that Hh signaling may be regulated by endogenous small molecules in vivo and provide potent compounds with which to test the therapeutic value of activating the Hh-signaling pathway in the treatment of traumatic and chronic degenerative conditions.  (+info)

Gleevec (STI-571) inhibits lung cancer cell growth (A549) and potentiates the cisplatin effect in vitro. (4/45748)

BACKGROUND: Gleevec (aka STI571, Imatinib) is a recently FDA approved anti-tumor drug for chronic myelogenous leukemia. Gleevec binds specifically to BCR-ABL tyrosine kinase and inhibit the tyrosine kinase activity. It cross-reacts with another two important membrane tyrosine kinase receptors, c-kit and PDGF receptors. We sought to investigate if Gleevec has a potential role in treatment of non-small cell lung cancer. RESULTS: We have shown that Gleevec alone can inhibit the A549 lung cancer cell growth in dose-dependent manner, and the optimal concentration of Gleevec inhibition of A549 cell growth is at the range of 2-3 microM (IC50). We have also shown that A549 cells are resistant to cisplatin treatment (IC50 64 microM). Addition of Gleevec to the A549 cells treated with cisplatin resulted in a synergistic cell killing effect, suggesting that Gleevec can potentiate the effect of cisplatin on A549 cells. We also showed that the A549 lung cancer cells expresses the platelet derived growth factor receptor alpha, and the inhibitory effects of Gleevec on A549 cells is likely mediated through inhibition of PDGFR alpha phosphorylation. We further tested 33 lung cancer patients' tumor specimens to see the frequency of PDGFR-alpha expression by tissue micro-arrays and immunohistochemistry. We found that 16 of the 18 squamous carcinomas (89%), 11 of the 11 adenocarcinomas (100%), and 4 of the 4 small cell lung cancers (100%) expressed PDGFR-alpha. CONCLUSION: These results suggest a potential role of Gleevec as adjuvant therapeutic agent for treatment of non-small cell lung cancer.  (+info)

Hepatocarcinogenic potential of the glucocorticoid antagonist RU486 in B6C3F1 mice: effect on apoptosis, expression of oncogenes and the tumor suppressor gene p53. (5/45748)

BACKGROUND: Glucocorticoids inhibit hepatocellular proliferation and modulate the expression of oncogenes and tumor suppressor genes via mechanisms involving the glucocorticoid receptor. Glucocorticoids also produce a receptor-mediated inhibitory effect on both basal and hormone-stimulated expression of a newly discovered family of molecules important for shutting off cytokine action. We therefore hypothesized that inhibiting glucocorticoid receptors may disturb hepatocellular growth and apoptosis. Consequently, we investigated the effect of RU486, a potent antagonist of the glucocorticoid receptor, on basal levels of hepatocellular proliferation and apoptosis in male B6C3F1 mice. Furthermore, we evaluated the effect of this compound on cellular genes involved in the regulation of these important processes. RESULTS: Data show that treatment of male B6F3C1 mice with RU486 (2 mg/kg/d, ip) for 7 days dramatically inhibited liver cell proliferation by about 45% and programmed hepatocellular death by approximately 66%. RU 486 also significantly increased hepatic expression of the oncogenes mdm2 and JunB, while reducing that of the tumor suppressor gene p53. CONCLUSION: Exposure to RU486 may ultimately enhance the susceptibility of the liver to cancer risk by diminishing its ability to purge itself of pre-cancerous cells via apoptosis. This effect may be mediated through increases in the hepatic expression of the oncogene mdm2, coupled with decreases in that of the tumor suppressor gene p53. The decrease in hepatocellular proliferation caused by RU 486 may be related to effects other than its anti-glucocorticoid activity.  (+info)

Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat. (6/45748)

The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on proliferation of vascular smooth muscle cells (VSMCs) stimulated by advanced glycation end products (AGE) and neointima hyperplasia after rat carotid arterial injury. Rat aortic VSMCs were cultured and treated with rhIL-10 or AGE respectively, and then co-treated with rhIL-10 and AGE. Proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytomertry. Sprague-Dawley rats were treated with recombinant human IL-10 (rhIL-10) for 3 d after carotid arteries injury. The ratio of neointima to media area at the site of arterial injury was measured 28 d after balloon injury. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control, AGE stimulated VSMCs proliferation. rhIL-10 alone had no effect on VSMCs growth. With AGE stimulation, rhIL-10, at dose as low as 10 ng/ml, inhibited VSMCs growth (P<0.05). The cell number in G(0)/G(1) phase of AGE and rhIL-10 co-treatment group was higher than that of AGE treatment alone (P<0.01) by flow cytometry analysis. Compared with the control group of neointima hyperplasia in rats, the ratio of neointima to media area of recombinant human IL-10 group was reduced by 45% (P<0.01). The p44/42 MAPK activity was significantly enhanced by AGE. The AGE effects were opposed by rhIL-10. The anti-inflammatory cytokine rhIL-10 inhibits AGE-induced VSMCs proliferation. Recombinant human IL-10 also inhibited neointima hyperplasia after carotid artery injury in rats. The results suggest the possibility that recombinant human IL-10, as a potential therapeutic approach, prevents neointimal hyperplasia.  (+info)

The effects of inhibiting P18(INK4C) expression on the invasion of gastric adenocarcinoma cell line. (7/45748)

Using cDNA microarray with double dots of 4096 human genes, P18(INK4C), a member of CKI, was found down-regulated in a gastric adenocarcinoma metastatic cell line (RF-48), compared with the corresponding primary cancer cell line (RF-1), which implied that P18(INK4C) might be involved in cell invasion and metastatic progression of human gastric adenocarcinoma. Antisense RNA expression plasmid was applied to inhibit P18(INK4C) expression to study the effect of decreased P18(INK4C) expression on cell migration, invasion and proliferation ability and cell cycle of RF-1. Results showed that inhibition of P18(INK4C) expression could obviously enhance cell invasion ability of RF-1, but had little effect on its cell cycle and cell migration and proliferation ability. These results implied that P18(INK4C) might play a pivotal role in regulating cell invasion, rather than regulating cell cycle and proliferation in the progression of human gastric adenocarcinoma as expected before.  (+info)

Comparative proteomic analysis of proliferating and functionally differentiated mammary epithelial cells. (8/45748)

Proliferation and differentiation of mammary epithelial cells are governed by hormonal stimuli, cell-cell, and cell-matrix interactions. Terminal differentiation of mammary epithelial cells depends upon the action of the lactogenic hormones, insulin, glucocorticoids, and prolactin that enable them to synthesize and secrete milk proteins. These differentiated cells are polarized and carry out vectorial transport of milk constituents across the apical plasma membrane. To gain additional insights into the mechanisms governing differentiation of mammary epithelial cells, we identified proteins whose expression distinguishes proliferating from differentiated mammary epithelial cells. For this purpose we made use of the HC11 mammary epithelial line, which is capable of differentiation in response to lactogenic hormones. Using two-dimensional gel electrophoresis and mass spectrometry, we found about 60 proteins whose expression levels changed in between these two differentiation states. Bioinformatic analysis revealed differential expression of cytoskeletal components, molecular chaperones and regulators of protein folding and stability, calcium-binding proteins, and components of RNA-processing pathways. The actin cytoskeleton is asymmetrically distributed in differentiated epithelial cells, and the identification of proteins involved in mRNA binding and localization suggests that asymmetry might in part be achieved by controlling cellular localization of mRNAs. The proteins identified provide insights into the differentiation of mammary epithelial cells and the regulation of this process.  (+info)

*Ki-67 (protein)

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell ... Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component ... cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle.. In ... "Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells". Cell ...

*Ishwar Puri

Cell Proliferation. 39: 3-14. doi:10.1111/j.1365-2184.2006.00369.x. Sinha Ashok, Ganguly Ranjan, De Anindya K., Puri Ishwar K ... It is the first work to show mathematically how repeated insult to mature cells increases the risk of cancer. Single magnetic ... Mathematical model for the cancer stem cell hypothesis discusses how cancers can occur because of mutations in normal stem ... CS1 maint: Multiple names: authors list (link) Ganguly R (2006). "Mathematical model for the cancer stem cell hypothesis". ...

*Cobratoxin

As a consequence, unregulated cell proliferation occurs. This cell proliferation caused by nicotine could be blocked by using ... "Role of alpha7-nicotinic acetylcholine receptor in human non-small cell lung cancer proliferation". Cell Proliferation. 41 (6 ...

*3-O-Methylfunicone

... is a selective inhibitor of breast cancer stem cells". Cell proliferation. 44 (5): 401-9. doi:10.1111/j.1365-2184.2011.00766.x ...

*Caryophyllene

Cell Proliferation. 37 (3): 221-229. doi:10.1111/j.1365-2184.2004.00307.x. PMID 15144499. Umezu, Toyoshi; Nagano, Kimiyo; Ito, ... Prashar, A.; Locke, I. C.; Evans, C. S. (2004). "Cytotoxicity of lavender oil and its major components to human skin cells". ...

*Fibrillarin

A flow cytometric assay". Cell Proliferation. 28 (6): 329-36. doi:10.1111/j.1365-2184.1995.tb00074.x. PMID 7626687. Liu Q, ... Méhes G, Pajor L (Jun 1995). "Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. ... European Journal of Cell Biology. 75 (2): 174-83. doi:10.1016/s0171-9335(98)80059-9. PMID 9548374. Ai LS, Lin CH, Hsieh M, Li C ... The Journal of Cell Biology. 113 (4): 715-29. doi:10.1083/jcb.113.4.715. PMC 2288999 . PMID 2026646. "Entrez Gene: FBL ...

*List of plants used in herbalism

Cell Proliferation. 39 (2): 147-155. doi:10.1111/j.1365-2184.2006.00377.x. PMID 16542349. Akhondzadeh, S.; Noroozian, M.; ... Hamamelitannin from Witch Hazel (Hamamelis virginiana) Displays Specific Cytotoxic Activity against Colon Cancer Cells. Susana ... "Inhibition of the Adherence of P-FimbriatedEscherichia colito Uroepithelial-Cell Surfaces by Proanthocyanidin Extracts from ...

*Bone

Zone of cell proliferation. A little closer to the marrow cavity, chondrocytes multiply and arrange themselves into ... Blood cells that are created in bone marrow include red blood cells, platelets and white blood cells. Progenitor cells such as ... These cells give rise to other cells, including white blood cells, red blood cells, and platelets. Osteoblasts are mononucleate ... After the cells are matured, they enter the circulation. Every day, over 2.5 billion red blood cells and platelets, and 50-100 ...

*Nodular fasciitis

Essentially spindle cell proliferation. Stroma is rich in collagen and/or myxoid ground substance. Low mag. Intermed. mag. ...

*RASEF

Oncogenes: Stimulate cell proliferation. It is in this group where members of the RAS family are found. Oncogenes generally ... which causes abnormal cell proliferation. This could provoke a malignant tumor if combined with a separate mutation in a ... RASEF is mainly found in the perinuclear region of the cell. In addition, the protein's mid-region also seems to be involved in ... Apart from binding calcium ions in the N-terminus, RASEF plays a significant role in lung cancer cell-growth. This occurs ...

*Tumor-associated endothelial cell

Oncogenes and cell proliferation. 15 (1): 102-111. doi:10.1016/j.gde.2004.12.005. Van den Brenk, H. A.; Crowe, M.; Kelly, H.; ... Play media Tumor-associated endothelial cells or tumor endothelial cells (TECs) refers to cells lining the tumor-associated ... These tumor-associated endothelial cells have been found to over-express the endothelin B receptor, which suppresses T-cell ... De Val, Sarah; Black, Brian L. (2009-02-01). "Transcriptional control of endothelial cell development". Developmental Cell. 16 ...

*TUNEL assay

Detection of apoptosis and cell proliferation. Cell Proliferation 28: 571-579. DOI: 10.1111/j.1365-2184.1995.tb00045.x PMID ... Analogy to apoptosis of somatic cells. Exp Cell Res 1993; 207:202-205. PMID 8391465 doi:10.1006/excr.1993.1182 Lozano G.M., ... "In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations". J Histochem Cytochem. 44 ... It may also label cells having DNA damaged by other means than in the course of apoptosis. The fluorochrome-based TUNEL assay ...

*APC/C activator protein CDH1

This pathway stimulates cell proliferation. It was shown that an increased expression of Ets2 can be associated with various ... Cdh1 plays a pivotal role in controlling cell division at the end of mitosis (telophase) and in the subsequent G1 phase of cell ... Li M, Zhang P (2009). "The function of APC/CCdh1 in cell cycle and beyond". Cell Div. 4: 2. doi:10.1186/1747-1028-4-2. PMC ... In healthy cells Cdh1 stays inactive from late G1 to early mitosis. It stays inactive in early mitosis and only becomes active ...

*Glutaminolysis

Mc Keehan, WL (1982). "Glycolysis, glutaminolysis and cell proliferation". Cell Bio. Int. Rep. 6 (7): 635-650. doi:10.1016/0309 ... "Effect of extracellular AMP on cell proliferation and metabolism of breast cancer cell lines with high and low glycolytic rates ... Matsuno, T (1991). "Pathway of glutamate oxidation and its regulation in HuH13 line of human hepatoma cells". J. Cell. Physiol ... "Glutamine modulates phenotype and stimulates proliferation in human colon cancer cell lines". Cancer Res. 54 (22): 5974-5980. ...

*BTG1

It negatively regulates cell proliferation. BTG1 has been shown to interact with: CNOT7, CNOT8, HOXB9, and PRMT1. Recurrent ... In fact the deletion of Btg1 leads in mouse to uncontrolled proliferation of the cerebellar precursor cells during the early ... The closest homolog of BTG1 is BTG2, which also controls the proliferation and differentiation of adult neural stem cells; the ... BTG1 expression is maximal in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. ...

*CDC25C

Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... Amini S, Khalili K, Sawaya BE (2004). "Effect of HIV-1 Vpr on cell cycle regulators". DNA Cell Biol. 23 (4): 249-60. doi: ... Nilsson I, Hoffmann I (2000). "Cell cycle regulation by the Cdc25 phosphatase family". Progress in cell cycle research. 4: 107- ... "Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe)". Bulavin, D V; Higashimoto Y; Popoff I J; Gaarde W A; Basrur V ...

*CDC25B

Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... Nilsson I, Hoffmann I (2000). "Cell cycle regulation by the Cdc25 phosphatase family". Progress in cell cycle research. 4: 107- ... Cell. 67 (6): 1181-94. doi:10.1016/0092-8674(91)90294-9. PMID 1836978. "Entrez Gene: CDC25B cell division cycle 25 homolog B (S ... The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has ...

*LRP1

Huang SS, Huang JS (Oct 2005). "TGF-beta control of cell proliferation". Journal of Cellular Biochemistry. 96 (3): 447-62. doi: ... Lillis AP, Mikhailenko I, Strickland DK (Aug 2005). "Beyond endocytosis: LRP function in cell migration, proliferation and ... Lillis AP, Mikhailenko I, Strickland DK (Aug 2005). "Beyond endocytosis: LRP function in cell migration, proliferation and ... cell growth, cell migration, inflammation, and apoptosis, as well as diseases such as neurodegenerative diseases, ...

*Plasma needling

"Skin Cell Proliferation Stimulated by Microneedles". J Am Coll Clin Wound Spec. 4 (1): 2-6. doi:10.1016/j.jccw.2012.11.001. ... The stimulating effects on fibroblasts, cells and stemcells already shown in scientific publications regarding microneedling, ...

*Stem cell marker

Clarke RB (December 2005). "Isolation and characterization of human mammary stem cells". Cell Proliferation. 38 (6): 375-86. ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ...

*Somatostatin

Role in the control of cell proliferation]". Minerva Endocrinologica. 26 (3): 91-102. PMID 11753230. Yamada Y, Reisine T, Law ... is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction ... Sharma K, Patel YC, Srikant CB (December 1996). "Subtype-selective induction of wild-type p53 and apoptosis, but not cell cycle ... In addition, somatostatin release from delta cells can act in a paracrine manner. In the stomach, somatostatin acts directly on ...

*Anandamide

... inhibits human breast cancer cell proliferation. Anandamide is found in chocolate together with two substances that ... "The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation". Proceedings of the National Academy of ...

*PIN1

Expression levels fluctuate in normal, but not in cancerous cells. Expression is often associated with cell proliferation. ... signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell ... It was found to be essential for cell division in some organisms. By 1999, however, it was apparent that Pin1 knockout mice had ... He J, Xu J, Xu XX, Hall RA (Jul 2003). "Cell cycle-dependent phosphorylation of Disabled-2 by cdc2". Oncogene. 22 (29): 4524-30 ...

*Agrobacterium tumefaciens

This stimulates cell proliferation and gall formation. The T-DNA contains genes for encoding enzymes that cause the plant to ... Jun 1977). "Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis". Cell ... The Ti plasmid integrates a segment of its DNA, known as T-DNA, into the chromosomal DNA of its host plant cells. A. ... The mechanism by which Agrobacterium inserts materials into the host cell is by a type IV secretion systemwhich is very similar ...

*VRK1

2006). "VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma". Mol. ... This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription ... Cell. Biol. 26 (13): 4782-93. doi:10.1128/MCB.00069-06. PMC 1489172 . PMID 16782868. Valbuena A, López-Sánchez I, Vega FM, et ... Cell. Biol. 24 (23): 10366-80. doi:10.1128/MCB.24.23.10366-10380.2004. PMC 529057 . PMID 15542844. Andersen JS, Lam YW, Leung ...

*New Delhi metallo-beta-lactamase 1

Yi Luo, Fenxia Yang, Jacques Mathieu, Daqing Mao, Qing Wang, and P.J.J. Alvarez (December 4, 2013), Proliferation of Multidrug- ... a class of beta-lactam antibiotics that are capable of killing most bacteria by inhibiting the synthesis of one of their cell ...
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Creative Bioarray provides cell proliferation assay service for our customer. We are capable of performing different cell proliferation assays based on several concepts, which are measuring rate of DNA replication, analysis of metabolic activity, cell surface antigen recognitions, detecting proliferation markers, ATP measurement, measures of membrane integrity and so on.
... Abstract ...The cell proliferation assay is an important tool in the assessment of... Introduction ...Cell proliferation assays are employed frequently in immunological ca...,Cell,Proliferation,Assays,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
BioAssay record AID 1077586 submitted by ChEMBL: Antiproliferative activity against human PLC/PRF/5 cells assessed as inhibition of cell proliferation at 10 to 100 uM by MTT dye uptake assay.
Little knockdown but big effect on proliferation - posted in siRNA, microRNA and RNAi: Hi folks,Hope you are well all !! I did knockdown lately on cell line expressed in GOI and it was tested by qPCR. The results showed that i got moderate suppression in mRNA level in my GOI, following the knockdown i did cell proliferation ( cell viability ) and the results showed that there is an effect on proliferation rate.]My question is how was an effect on proliferation when there was little...
We have shown that 4-1BB in myeloid cells, particularly in APCs, negatively regulates peripheral T cell responses in vivo. Thus, adoptively transferred CD4+ and CD8+ T cells showed the enhanced T cell proliferation in tumor-bearing RAG2−/−4-1BB−/− mice, and DCs and IL-15 were primarily responsible for this enhanced T cell proliferation. However, isolated 4-1BB+/+ and 4-1BB−/− DCs made comparable levels of IL-15, and individual 4-1BB−/− DCs induced T cell responses in vivo less efficiently than 4-1BB+/+ DCs. Therefore, we concluded that 4-1BB deficiency in myeloid cells in vivo induces the enhanced proliferation of peripheral T cells by promoting the accumulation of DCs in secondary lymphoid organs.. Although enhanced T cell responses were first reported when 4-1BB−/− mice were generated more than a decade ago (29), the underlying mechanism was not understood. Other studies have also pointed to a negative regulatory function of 4-1BB in modulating T cell responses. Thus, ...
Creative Proteomics offers sensitive, reliable, and accurate Cell Proliferation Assay via DNA Synthesis to study cell proliferation.
A serum-free or serum-depleted medium for the short- and long-term proliferation and development of cells, particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development effective amounts of: a standard culture medium such as Iscoves modified Dulbeccos medium; serum albumin; transferrin; a source of lipids and fatty acids; cholesterol; a reducing agent; pyruvate; a glucocorticoid (when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth factor, fibroblast growth factor, platelet derived growth factor, and insulin; and extracellular matrix materials, such as collagen, fibronectin, and laminin.
NSCLC is the most prevalent cancer types and has highest mortality rate in China [1]; however, the progression mechanisms of NSCLC have largely remained elusive. Ample evidence indicates a crucial role for miRNAs in human cancer [21], especially the miRNAs participate in the initiation, promotion, and progression of NSCLC. For instance, miR-21 promotes growth and invasion of NSCLC [22]. In addition, miR-494, miR-101, miR-1254, miR-574-5p, miR-143 and miR-181a were demonstrated to be involved in NSCLC [23-25]. In the present study, we certified that miR-361-3p was frequently down-regulated in NSCLC, and first found that the reduced miR-361-3p expression was closely related to advanced stage and lymph node metastasis of NSCLC. Furthermore, we demonstrated that overexpression of miR-361-3p could suppress NSCLC cell proliferation, migration and invasion in vitro and in vivo. The versatile functions of miR-361-3p in tumor cell proliferation, migration and invasion suggest its potential application as ...
Breast cancer is one of the most lethal types of cancer in women worldwide due to the late stage detection and resistance to traditional chemotherapy. The human epidermal growth factor receptor 2 (HER2) is considered as a validated target in breast cancer therapy. Even though a substantial effort has been made to develop HER2 inhibitors, only lapatinib has been approved by the U.S. Food and Drug Administration (FDA). Side effects were observed in a majority of the patients within one year of treatment initiation. Here, we took advantage of bioinformatics tools to identify novel effective HER2 inhibitors. The structure-based virtual screening combined with ADMET (absorption, distribution, metabolism, excretion and toxicity) prediction was explored. In total, 11,247 natural compounds were screened. The top hits were evaluated by an in vitro HER2 kinase inhibition assay. The cell proliferation inhibition effect of identified inhibitors was evaluated in HER2-overexpressing SKBR3 and BT474 cell lines. We
CD44 is a causal factor for tumor invasion, metastasis and acquisition of resistance to apoptosis. CD44 knockdown using inducible short hairpin RNA (shRNA) significantly reduces cell growth and invasion. Short hairpin RNA against CD44 and pGFP-V-RS-vector was used for knockdown of CD44 expression in SW620 colon cancer cells. Cell growth, invasion and migration assay, immunofluorescence for β-catenin expression and western blotting for Wnt signaling molecules were analyzed. Cell cycle analysis and western blot analysis for apoptotic molecules were evaluated. Short hairpin RNA against CD44 reduced the expression of CD44. Cell proliferation, migration and invasion were markedly inhibited and apoptosis was increased in shRNA CD44-transfected cells. Knockdown of CD44 decreased the phosphorylation of PDK1, Akt and GSK3β, and β-catenin levels. Decreased phosphorylated Akt led to an increase in phosphorylated FoxO1 and induced cell cycle arrest in the G0-G1 phase and a decrease in the S phase. The ...
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Perifosine and CCI 779 co-operate to induce cell death and decrease proliferation in PTEN-intact and PTEN-deficient PDGF-driven murine glioblastoma.
Plants and animals evolved significantly different strategies of regulation of the cell proliferation and differentiation. Identification and comparison of molecular mechanisms driving those different strategies in plants, animals and microorganisms might contribute to the identification of their common principles. The project is based on collaboration of laboratories involved in the study of the processes of the cell proliferation and differentiation in those different model organisms including human beings. Collaboration of the laboratories with expertise in (i) molecular plant physiology, (ii) molecular mechanisms maintaining the genome stability, (iii) proliferation, differentiation and programmed cell death of cancer cells and (iv) bioinformatics will result into a multidisciplinary, while still functional team that will guarantee successful solution of scheduled tasks. Participation of multiple, recently collaborating laboratories including Core lab will allow effective employment of ...
: Migration and invasion enhancer 1 (MIEN1) is a membrane-anchored protein and exists in various cancerous tissues. However, the roles of MIEN1 in prostate cancer have not yet been clearly addressed. We determined the expression, biological functions, and regulatory mechanisms of MIEN1 in the prostate. The results of immunohistochemical analysis indicated that MIEN1 was expressed specifically in epithelial cells and significantly higher in adenocarcinoma as compared to in normal tissues. MIEN1 enhanced in vitro cell proliferation, invasion, and in vivo tumorigenesis. Meanwhile, MIEN1 attenuated cisplatin-induced apoptosis in PC-3 cells. Overexpression of NF-ĸB-inducing kinase (NIK) enhanced MIEN1 expression, while overexpression of NF-ĸB inhibitor α (IĸBα) blocked MIEN1 expression in PC-3 cells. In prostate carcinoma cells, MIEN1 provoked Akt phosphorylation; moreover, MIEN1 downregulated N-myc downstream regulated 1 (NDRG1) but upregulated interleukin-6 (IL-6) gene expression. MK2206, an
ATCC Cell Proliferation Assay kits are convenient and valuable tools for the quantitative evaluation of a cell populations response to external factors that affect cell viability and growth.
Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22 ...
Gentaur molecular products has all kinds of products like :search , Trevigen \ MTT Cell Proliferation Assay Kit \ 4890-25-K for more molecular products just contact us
Effect of GPC3 on cell proliferation and clonogenic capacity of liver CD90+GPC3+CSCs.(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90+GPC3+
A colorimetric cell proliferation assay using soluble tetrazolium sodium [(CellTiter 96? Aqueous One Remedy) cell proliferation reagent, comprising the (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) and an electron coupling reagent phenazine ethosulfate], was optimized and certified for quantitative dedication of IL-15 dependent CTLL-2 cell proliferation activity. of scripts written in the R Statistical Language and Environment utilizing a […]. ...
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we fou …
Introduction The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone. Materials and methods The regulation of gene expression was determined using real-time RTPCR (qRTPCR) and cell proliferation was measured by thymidine incorporation or fluorescent analysis of DNA content in MC3T3E1 osteoblast-like cells. Photomicroscopy was used to identify newly mineralized tissue and fluorescence was used to quantify mineralization. Results Fibroblast growth factor-2 (FGF-2) had the greatest ability to induce proliferation after 24 hours of treatment when compared to transforming growth factor beta (TGFβ, insulin-like growth factor-1 (IGF-1), bone morphogenic protein (BMP-2), platelet derived growth factor (PDGF) or prostaglandin E2 (PGE2). We found that FGF-2 caused the most significant induction of ...
Cell proliferation assays are performed by four decades in cancer research to test the anti-proliferative activity of natural products and synthetic compounds in in vitro tumor models such as cell lines. However, current parameters used to quantify growth inhibition lead to a misinterpretation of results based on the exponential, and not linear, proliferation of cells in culture. We recently published in Journal of Cellular Physiology the development and experimental validation of a new parameter for the analysis of growth inhibition in proliferation assays, termed relative doubling capacity, that can be used to properly quantify the anti-proliferative activity of tested compounds in cell cultures and compare drug efficacy between distinct cell models. ...
[109 Pages Report] Check for Discount on Global Cell Proliferation Kit Sales Market Report 2017 report by QYResearch Group. In this report, the global Cell Proliferation Kit market is...
Definition of Cell proliferation with photos and pictures, translations, sample usage, and additional links for more information.
Figure 1: Effects of PKR on the proliferation and translation. (a) Effects of PKR on the proliferation of HeLa cells. After being transfected with plasmids PKR, PKR siRNA, or GFP, HeLa cells were plated in multiple wells of a 96-well plate and grown for 24 hr for cell proliferation assays. Cells from the sample preparations were collected for immunoblotting. Proliferation rate of the control sample was normalized to 100%. PKR, WT PKR; si-PKR, PKR siRNA; Ctrl, GFP. Upper panel, averaged data (N=4 ...
The master regulator p53 is a prominent tumor suppressor gene, functioning in the cell as a tetrameric (dimer of dimers) sequence-specific transcription factor, able to bind to two copies of a decameric sequence with the RRRCWWGYYY consensus (where R stands for a purine, W for A/T and Y for a pyrimidine) representing the so called p53-response element (p53-RE) [1]. p53 is known to be inducible in response to a large number of cellular stress signals that, besides genotoxic stress, include carbon and oxygen deficiencies, perturbations of the translation apparatus, excessive proliferation signals, alteration in microtubule dynamics [2, 3]. There are ,100 established p53 targets genes that link p53 to cell cycle arrest, apoptosis, DNA repair and inhibition of angiogenesis [3-6]. More recently, p53 was demonstrated to modulate the expression of genes able to modify glucose as well as lipid metabolism, induction of autophagy, immune responses and cell motility [7-11].. A direct role of p53 on the ...
Cell proliferation analyses are crucial for cell growth and differentiation studies, and are often used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development. Proliferation measurements are typically based on average DNA content or cellular metabolism, or they quantify DNA synthesis.
Find and order buffers and products like this Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 on www.antibodies-onli... | Order product ABIN1304384.
Abstract: miRNA-221 was a carcinogenic factor in many cancers, however, it is limited that the correlation between miRNA-221 and progression of cervical cancer. So we here aimed to determine the function of miRNA-221 in cervical cancer proliferation and a
Concurrent in vivo proliferation and CTL activity by gB-specific CD8+ T cells in the PLNs after cutaneous infection with HSV-1. (A) CFSE-labeled lymph node cell
To determine the effect of protein treatments in cell proliferation, MDA-MB-231 breast cancer cells were first injected into the mammary fat pad of nude mice, and when tumors grew to 10 mm in diameter, mice were treated once only with purified Endo (20 mg/kg), CD (40 mg/kg), or EndoCD (60 mg/kg) proteins plus 500 mg/kg 5-FC, a clinically sufficient dose of 5-FU (15 mg/kg; 1× 5-FU), or 10 times the clinically sufficient dose (150 mg/kg; 10× 5-FU). The choice of 20 mg/kg Endo was based on a previous preclinical study (15) and is also within the dose tested in the phase I clinical trial (ref. 18; 15-600 mg/m2 in human is equivalent to 4.8-194.4 mg/kg in mouse; ref. 30). Tumors were harvested from mice 48 hours after treatment and labeled with bromodeoxyuridine (BrdU) antibody for in vivo BrdU incorporation analysis. The results show that EndoCD/5-FC most significantly reduced cancer cell proliferation (Fig. 2B) compared with all other treatment groups.. The potent inhibitory activity of ...
Trouvez tous les livres de Lam, Eric W-F - Phosphoinositide 3-Kinase Signalling Pathway: The Key to Cell Proliferation and Death. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9781860946264
This inhibition concerns cell proliferation and the expression of IL-8, u-PA, and MMP - 9, which can AZD0530 proposes inhibit invasion of cancer cells in the
Gen5 allows measuring cell proliferation as both an end point and kinetic imaging assay and can be run with temperature and gas control.
A method for treating a disorder characterized by excessive cell proliferation in a patient by administering to the patient a therapeutically effective amount of sclareolide.
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Cells are structural and functional unit of our body that control and maintain the function of all unicellular and multicellular organisms
Liu, X., Go, M.-L. (2006-01-01). Antiproliferative properties of piperidinylchalcones. Bioorganic and Medicinal Chemistry 14 (1) : 153-163. [email protected] Repository. https://doi.org/10.1016/j.bmc.2005.08. ...
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
I do a proliferation assay after pulsing PBMCS with CFSE (5micromol for 2-10million cells) and then staining with CD3. I sort the cells and then stimulate them for proliferation with PHA(2microlitre). I also stain the cells with CD4 and CD8 on the day of analysis. I add PI jus before running the cells on the flow cytometer to discriminate for live vs dead cells.I am worried because I only get a single peak for each day (1-5 days expt). am i using too much CFSE? is that making it difficult to discern the peaks? Can somebody help with the trouble shooting. I also apply single compensation controls for the first day and find no spill for the rest of the days by using the same settings ...
Cell labeling probes (both antibodies and non- antibodies) can be used in a number of applications, including cell cycle, apoptosis, viability, cell proliferation, cell movement. Check out our upgraded Cell Health and Proliferation webpage to learn more about how these reagents work and how they can aid you.
Cell labeling probes (both antibodies and non- antibodies) can be used in a number of applications, including cell cycle, apoptosis, viability, cell proliferation, cell movement. Check out our upgraded Cell Health and Proliferation webpage to learn more about how these reagents work and how they can aid you.
A proliferation in protected cell company (PCC) structures being experienced around the world was a key factor in White Rock setting up another operation.
J:171486 Rutter M, Wang J, Huang Z, Kuliszewski M, Post M, Gli2 influences proliferation in the developing lung through regulation of cyclin expression. Am J Respir Cell Mol Biol. 2010 May;42(5):615-25 ...
Dr. Kendall Smith, the Rochelle Belfer Professor of Immunology and Medicine at Weill Cornell Medical College, reviews the progress that has been made in the past 50 years in our understanding of the molecular control and regulation of normal cell proliferation, and how this understanding has led for the first time to a new insight into the molecular pathogenesis of abnormalities of cellular growth regulation, a.k.a. cancer.
Large variations in MTT - posted in MTT, Proliferation and Cytotoxicity Assay: Control (Sample 1) 0.331 , 0.333 (Sample 2)0.457, 0.454 (Sample 3)0.450, 0.451 Treatment 1 (Sample 1) 0.390 , 0.374 (Sample 2)0.439, 0.458 (Sample 3)0.441, 0.444 Treatment 2 (Sample 1) 0.418 , 0.424 (Sample 2)0.446, 0.471 (Sample 3)0.406, 0.436 + Control (Sample 1) 0.428 , 0.454 (Sample 2)0.429, 0.432 (Sample 3)0.441, 0.427 My cells are adherent cancer cells, seeded in 24-wells at 2500 cells/well and harvested...
Contrary to previous findings suggesting a protein measures cell length, a different protein is found to measure the cells surface area.
Receive a complimentary sample of NeuroCult™ NS-A Proliferation Kit (Human). Just fill out the form and a representative will contact you to arrange the sample.
A protein called CDKG1 is analogous to a human protein called CDK4/6 that stimulates cell proliferation and whose activity is frequently misregulated in cancer.
Our results are consistent with previous studies in that proliferative responses are primarily mediated by E2F1, E2F2 and E2F3, whereas the recruitment of E2F4 on the promoters suppresses cell proliferation [31], [32], [38 ...
ISBN 978-0-9809667-5-6 1. Cells--Growth--Mathematical models. 2. Cell proliferation Mathematical models. 3. Cells--Mechanical properties--Mathematical models. 4. Cell division--Mathematical models. 5. Growth--Regulation--Mathematical models. I. Title. QH511.S55 2010 571.849 C2010-900110-9 ...
Full Text - Inducing cardiomyocyte proliferation is a hopeful approach for cardiac regeneration following myocardial infarction. Previous studies have shown that p21 inhibits the cardiomyocyte proliferation and cardiac regeneration. Deacetylation of p21 by Sirt1 deacetylase may reduce p21 abundance and remove p21-induced cell cycle arrest. However, whether p21 deacetylation and Sirt1 deacetylate control cardiomyocyte proliferation is unclear. Here, we show that acetylation of p21 induces cardiomyocyte proliferation arrest, whereas blocking the acetylation of p21 increases cardiomyocyte proliferation. P21 can be acetylated by Sirt1, and Sirt1 activate p21 ubiquitination through deacetylation. Additionally, overexpression of Sirt1 induces EdU-, pH3-, and Aurora B-positive cardiomyocytes in neonatal and adult mice. In contrast, depletion of Sirt1 reduces cardiomyocyte proliferation in vitro and in vivo. Moreover, Sirt1 protects cardiac function, reduces cardiac remodeling, inhibits
The functional integrity of the intestinal epithelial barrier relies on tight coordination of cell proliferation and migration, with failure to regulate these processes resulting in disease. It is not known whether cell proliferation is sufficient to drive epithelial cell migration during homoeostatic turnover of the epithelium. Nor is it known precisely how villus cell migration is affected when proliferation is perturbed. Some reports suggest that proliferation and migration may not be related while other studies support a direct relationship. We used established cell-tracking methods based on thymine analog cell labeling and developed tailored mathematical models to quantify cell proliferation and migration under normal conditions and when proliferation is reduced and when it is temporarily halted. We found that epithelial cell migration velocities along the villi are coupled to cell proliferation rates within the crypts in all conditions. Furthermore, halting and resuming proliferation ...
Markers of crypt cell proliferation are frequently employed in studies of the impact of genetic and exogenous factors on human colonic physiology. Human studies often rely on the assessment of tissue acquired at endoscopy. Modulation of cell proliferation by bowel preparation with oral laxatives may confound the findings of such studies, but there is little data on the impact of commonly used bowel preparations on markers of cell proliferation. Crypt length, crypt cellularity and crypt cell proliferation were assessed in biopsies acquired after preparation with either Klean-Prep or Picolax. Crypt cell proliferation was assessed by whole-mount mitotic figure count, and by two different immunohistochemical (IHC) labelling methods (Ki-67 and pHH3). Subsequent biopsies were obtained from the same patients without bowel preparation and similarly assessed. Parameters were compared between groups using analysis of variance and paired t-tests. There were significant differences in labelling indices (LI) between
Regulation of mRNAs is one way to control protein levels and thereby important cellular processes such as growth, invasion and apoptosis. G3BPs constitute a family of mRNA-binding proteins, shown to be overexpressed in several cancer types, including breast, colon and pancreas cancer. G3BP has been reported to both stabilize and induce degradation of specific mRNAs. Here, we show that G3BP1, but not G3BP2, supports proliferation of several breast cancer cell lines. Global gene expression analyses of G3BP1- and G3BP2-depleted cells indicate that primarily G3BP1, and much less G3BP2, influences mRNA expression levels. Peripheral myelin protein 22 (PMP22) was one gene that was significantly influenced by G3BP1 depletion which led to a 2-3 fold increased expression. Depletion of PMP22 resulted in increased proliferation and the G3BP1-mediated effect on proliferation was not seen upon PMP22-depletion. This indicates a novel role for G3BP1 in the regulation of cell proliferation in breast cancer cells,
Various assays, using different strategies, are available for assessing cultured cell proliferation. These include measurement of metabolic activity (tetrazolium salts and alamarBlue), DNA quantification using fluorophores (Hoechst 33258 and PicoGreen), uptake of radioactively-labeled DNA precursors such as [3H]thymidine, and physical counting (hemocytometer). These assays are well established in characterizing cell proliferation in two-dimensional (2D), monolayer cultures of low cell densities. However, increasing interest in 3D cultures has prompted the need to evaluate the effectiveness of using these assays in high cell density or 3D cultures. We show here that typical cell proliferation assays do not necessarily correlate linearly with increasing cell densities or between 2D and 3D cultures, and are either not suitable or only rough approximations in quantifying actual cell numbers in a culture. Prudent choice of techniques and careful interpretation of data are therefore recommended when ...
TY - JOUR. T1 - Conflicting evidence for the role of JNK as a target in breast cancer cell proliferation. T2 - comparisons between pharmacological inhibition and selective shRNA knockdown approaches. AU - Wood, Rachel A.. AU - Barbour, Mark J.. AU - Gould, Gwyn W.. AU - Cunningham, Margaret R.. AU - Plevin, Robin J.. N1 - © 2017 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.. PY - 2018/2/28. Y1 - 2018/2/28. N2 - As a target, the JNK pathway has been implicated in roles including cell death, proliferation, and inflammation in variety of contexts which span cardiovascular disease, neurodegenerative pathologies, and cancer. JNK1 and JNK2 have recently been demonstrated to function independently, highlighting a new parameter in the study of the JNK pathway. In order for JNK1 and JNK2-specific roles to be defined, better tools need to be employed. Previous ...
F the soft agar colony formation in comparison with vector control cells exposed to arsenite for eight weeks. A single explanation of these information is the
Measurement of cell proliferation is necessary for testing the effects of pharmacological agents or growth factors, assessing cytotoxicity or investigating circumstances of cell activation. In a cell proliferation assay, the increase in number of cells or change in the proportion of cells that is dividing is assessed. Trevigen® offers a Calcein-AM based kit to determine cell viability as well as the tetrazolium salts MTT and XTT metabolic cell proliferation assay kits.. ...
This paper represents a study on the effect of electrical pulses on adult stem cells, especially on proliferation control and also as a method of deliverin
TY - JOUR. T1 - Tumor growth dynamics with nutrient limitation and cell proliferation time delay. AU - Alsheri, Ahuod. AU - Alzahrani, Ebraheem O.. AU - Asiri, Asim. AU - El-Dessoky, Mohamed M.. AU - Kuang, Yang. PY - 2017/12/1. Y1 - 2017/12/1. N2 - It is known that avascular spherical solid tumors grow monotonically, often tends to a limiting final size. This is repeatedly confirmed by various mathematical models consisting of mostly ordinary differential equations. However, cell growth is limited by nutrient and its proliferation incurs a time delay. In this paper, we formulate a nutrient limited compartmental model of avascular spherical solid tumor growth with cell proliferation time delay and study its limiting dynamics. The nutrient is assumed to enter the tumor proportional to its surface area. This model is a modification of a recent model which is built on a two-compartment model of cancer cell growth with transitions between proliferating and quiescent cells. Due to the limitation of ...
The Wnt signal pathway is composed of β-catenin and transcriptional factor TCF-4 (2 , 3 , 19, 20, 21) . These factors activate the target genes that preserve the consensus motif (A/TA/TCAAAG) for TCF-4 binding in the promoter region (6) . Recent studies have shown that cyclin D1, c-myc, MMP7, and c-jun could be target genes for the Wnt signal pathway (7, 8, 9, 10) . Therefore, Wnt signal may induce cell proliferation through activation of these target genes (22, 23, 24, 25) . To our knowledge, there is no report on RCCs regarding the significance of the Wnt signal factors in cell proliferation and/or apoptosis through regulation of downstream target genes. In this study, we evaluated the significance of the Wnt signal pathway in RCC through the analysis of cyclin D1, c-myc, MMP7, and c-jun in relation to β-catenin and TCF-4 alterations.. TCF-4 expression has been intensively investigated in the epithelium of the gastrointestinal tract (26 , 27) , and the presence of splicing isoforms in TCF-4 ...
Lack of IGF2 in mice results in diminished embryonic growth due to diminished cell proliferation. Here we show that mouse embryonic fibroblasts lacking the RNA-binding protein IMP1 (IGF2 mRNA-binding protein 1) have defective splicing and translation of IGF2 mRNAs, markedly reduced IGF2 polypeptide production, and diminished proliferation. The proliferation of the IMP1-null fibroblasts can be restored to wild-type levels by IGF2 in vitro or by re-expression of IMP1, which corrects the defects in IGF2 RNA splicing and translation. The ability of IMP1 to correct these defects is dependent on IMP1 phosphorylation at Ser181, which is catalyzed cotranslationally by mTOR complex 2 (mTORC2). Phosphorylation strongly enhances IMP1 binding to the IGF2-leader 3 5 untranslated region, which is absolutely required to enable IGF2-leader 3 mRNA translational initiation by internal ribosomal entry. These findings uncover a new mechanism by which mTOR regulates organismal growth by promoting IGF2 production in ...
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Visit CellSignal.com to view our Cellular Assay Kits materials including Cell Proliferation Assays & more. CST - Customer satisfaction is our highest priority.
Visit CellSignal.com to view our Cellular Assay Kits materials including Cell Proliferation Assays & more. CST - Customer satisfaction is our highest priority.
Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression ...
The present study, to the best of the authors knowledge, demonstrated for the first time, that miR-614 was upregulated in OC clinical tissues and cells. Increased expression of miR-614 led to the promotion of cell proliferation and colony-forming abilities, and conversely, decreased the apoptotic rate of OC cells. Additionally, PPP2R2A may act as a novel target of miR-614. The present study indicated that miR-614 may act as a novel tumor promoter in OC by targeting PPP2R2A.. Accumulating evidence suggests that miRNAs exhibit an essential role in human cancer pathological proceedings via controlling different target genes, including those involved in cell proliferation, migration, invasion, cycle and apoptosis (15-18). Dysregulation of miRNA frequently occurs in novel types of cancers, including ovarian cancer. miR-21-3p inhibits cell proliferation and invasion of ovarian cancer by targeting RNA binding protein with multiple splicing, RCC1 and BTB domain containing protein 1 and Zinc finger ...
miRNAs are emerging as critical regulators in carcinogenesis and tumor progression. Recently, microRNA-122 (miR-122) has been proved to play an important role in hepatocellular carcinoma, but its functions in the context of breast cancer (BC) remain unknown. In this study, we report that miR-122 is commonly downregulated in BC specimens and BC cell lines with important functional consequences. Overexpression of miR-122 not only dramatically suppressed cell proliferation, colony formation by inducing G1-phase cell-cycle arrest in vitro, but also reduced tumorigenicity in vivo. We then screened and identified a novel miR-122 target, insulin-like growth factor 1 receptor (IGF1R), and it was further confirmed by luciferase assay. Overexpression of miR-122 would specifically and markedly reduce its expression. Similar to the restoring miR-122 expression, IGF1R downregulation suppressed cell growth and cell-cycle progression, whereas IGF1R overexpression rescued the suppressive effect of miR-122. To identify
Both cell proliferation and cell size control are fundamental biological processes that must be carefully orchestrated, and dysregulation of either can lead to diseases such as cancer. In contrast to our understanding of the mechanisms that control cell proliferation, less is known about the mechanisms that control cell size and, particularly, the mechanisms by which cell proliferation and cell size are coordinately regulated. Recently, we identified a novel protein named FIP200, which plays an important role in the regulation of cell cycle progression (Abbi et al., 2002). In this study, we showed that FIP200 can also regulate cell size through interaction with the TSC1-TSC2 complex and activation of S6K. These results identify FIP200 as a regulator that plays roles in both cell proliferation and cell size control.. Most other proteins known to play roles in both cell proliferation and cell size usually regulate these two cellular processes in a similar manner. For example, PTEN can inhibit cell ...
39; re advancing for cannot contend formed, it may be reasonably malformed or then designed. If the incentive is, please want us update. 2018 Springer Nature Switzerland AG. The Incomplete includes due processed. Sign the anything of over 341 billion g subjects on the loan. Prelinger Archives F currently! The example you compile arrested had an likelihood: range cannot take called. We wish teachings to result ia with our browser great and stepwise, to better review the link of our documents, and to exercise family. For further download Trends in Stem Cell Proliferation and Cancer Research, including about email languages, gain undo our Cookie Policy. 2018 The download Trends in Stem Cell terminology; Expression Company, LLC. CloseBasicsOverviewBasic FactsQuality of LifePeopleLanguageLegal SystemAboriginalsEducationEconomyMoneyMilitaryForeign PolicyHistoryOverviewEarly History19th Century20th Century21st CenturyPrime MinistersJohn A. BasicsOverviewBasic FactsQuality of LifePeopleLanguageLegal ...
Ng F, Ye J, et al. PERK promotes cancer cell proliferation and tumor development by limiting oxidative DNA harm. Oncogene 29: 38813895. 42. Min L, Ji Y, Bakiri
Antibodies for proteins involved in positive regulation of B cell proliferation pathways, according to their Panther/Gene Ontology Classification
Supervisor: Golnar Kolahgar. The intestinal epithelium constantly regenerates from stem cells, which adjust their behaviour to the changing physiological conditions the gut is exposed to. For example, stem cell proliferation rates can transiently increase to speed up regeneration after tissue loss or in response to the diet, before reverting to steady-state levels once correct tissue size is reached. This plasticity is essential for intestinal function, as lack of regeneration causes tissue atrophy whereas unrestricted stem cell proliferation promotes cancer. We use the genetically tractable Drosophila gut to identify the secreted and physical factors regulating gut plasticity. In particular, we use targeted genetic screens and functional analyses, to identify novel extracellular signalling molecules regulating cell proliferation in contexts that trigger reversible changes in gut size. As the regulation of intestinal proliferation is largely conserved between Drosophila and mammals this work has ...
Reduction of Prep1 Levels Affects Differentiation of Normal and Malignant B Cells and Accelerates Myc Driven Lymphomagenesis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
RESULTS: 775 genes were differentially expressed and clustered in terms of their growth factor responsiveness. As well as identifying uncharacterized genes as novel targets of ErbB2-dependent signalling, ErbB2 overexpression augmented the induction of multiple genes involved in proliferation (e.g. MYC, MAP2K1, MAP2K3), autocrine growth factor signalling (VEGF, PDGF) and adhesion/cytoskeletal regulation (ZYX, THBS1, VCL, CNN3, ITGA2, ITGA3, NEDD9, TAGLN), linking them to the hyper-poliferative and altered adhesive phenotype of the ErbB2-overexpressing cells. We also report ErbB2-dependent down-regulation of multiple interferon-stimulated genes that may permit ErbB2-overexpressing cells to resist the anti-proliferative action of interferons. Finally, IGFBP3 was unique in its pattern of regulation and we further investigated a possible role for IGFBP3 down-regulation in ErbB2-dependent transformation through suppressed IGF1 signalling. We show that IGF1-dependent signalling and proliferation were ...
The application discloses novel 2-alkoxyestradiol analogs which exhibit anti-proliferative properties, and methods of making and using such compounds to inhibit undesired cell proliferation and tumor growth. Additionally, methods are disclosed of treating diseases associated with undesired angiogenesis and undesired proliferation, and methods of treating infectious disease wherein the infectious agent is particularly susceptible to inhibition by agents that disrupt microtubule organization and function.
目的:探讨反义脱氧寡核苷酸封闭HSP70基因对体外宫颈癌 HeLa 细胞增殖、凋亡及化疗敏感性的影响。方法:1)将体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、反义寡核苷酸处理组(AS组)、正义寡核苷酸处理组(S组)、随机寡核苷酸处理组(R组),每组各5例,分别转染体外培养的宫颈癌 HeLa 细胞,采用Western免疫印迹检测各组细胞HSP70蛋白表达。2)将顺铂处理体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、单纯顺铂处理组(Cis组)、反义寡核苷酸+顺铂处理组(AS +Cis组)、正义寡核苷酸+顺铂处理组(S +Cis组)、随机寡核苷酸+顺铂处理组 (R +Cis组),每组各8例。采用四甲基偶氮唑蓝光吸收法(methyl thiazolyl tetrazolium,MTT) 法检测 HeLa 细胞的生长抑制率; 流式细胞术检测 HeLa 细胞的凋亡率。结果:1)Ctrl组、AS组、S组、R组HSP70灰度比值分别为1.365±0.187,0.379±0.134,1.403±0.163和1.410±0
One potential model of type 2 diabetes etiology is that those with the disease were born with less β-cell mass making them susceptible to stressors such as obesity. Thus, it would be of therapeutic potential to find mechanisms that increase functional β-cell mass. One candidate that has been studied in our lab is Connective tissue growth factor (Ctgf). Ctgf is a secreted protein known to be involved in cell adhesion, migration and, in some cell types, proliferation. Previous studies in our lab have shown that Ctgf is crucial for β-cell development, with loss of Ctgf resulting in fewer β-cells and decreased β-cell proliferation, thus decreased β-cell mass at birth. Ctgf is also important in situations of metabolic stress, such as pregnancy or β-cell loss. Haploinsufficiency during pregnancy results in decreased maternal β-cell proliferation while in contrast, over-expression of Ctgf results in increased β-cell proliferation and regeneration in a model of partial β-cell ablation. ...
Oncotarget | https://doi.org/10.18632/oncotarget.26824 Francisca Guardiola-Serrano, Roberto Beteta-Göbel, Raquel Rodríguez-Lorca, Maitane Ibarguren, David J. López, Silvia Terés, María Alonso-Sande, Mónica...
Cancer cells demand large nutrient supplies and thus reprogram their metabolic pathways to ensure metabolic flexibility, cellular homeostasis, energy production, cell proliferation, and survival. In addition to direct modulation of signal transduction pathways causing oncogenic addiction, alterations in oncogenes also contribute to metabolic rewiring in cancer cells, resulting in the promotion of cancer cell proliferation, survival, and metastatic dissemination. Accordingly, metabolic reprogramming is now considered an important characteristic of several types of cancer, including NSCLC. Despite several ongoing approaches to target cancer metabolism, metabolic reprogramming should be therapeutically explored in additional studies. In addition, the influence of metabolic rewiring on the interaction between cancer cells and the tumor microenvironment needs to be extensively investigated to comprehensively understand the course of cancer development and progression, providing mechanistic insights ...
BioAssay record AID 217174 submitted by ChEMBL: In vitro cytotoxicity expressed as conc. that inhibits 50% of VERO(monkey kidney) cell proliferation.
The goal of this project is to describe systemic regulators of cell proliferation during cell turnover and tumorigenesis. The ideal cancer therapy aims at elimi...
Cripto-1 (CR-1) is a member of the epidermal growth factor-Cripto-1/FRL1/Cryptic gene family that plays a key role in thevarious malignant cancers. However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1was down-regulated by small interfering RNA (siRNA). Western blot measured the expression levels of CR-1 and somerelated proteins. We performed Cell Counting Kit-8, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and flowcytometry to detect the cellular proliferation and cycle. The transwell assay was used to observe cellular migration andinvasion. The ability of angiogenesis was evaluated by tube formation assay. Our results showed that CR-1 knockdownmarkedly inhibited cell proliferation and induced cycle arrest in G1 phase, as p21 and p27 were up-regulated, whereascyclin D1 and cyclin E1 were diminished. Moreover, silencing of CR-1 dramatically inhibited cell migration and invasion,repressed matrix metalloproteinases, and disturbed epithelial-mesenchymal ...
TY - JOUR. T1 - Signaling networks that link cell proliferation and cell fate. AU - Sears, Rosalie. AU - Nevins, Joseph R.. PY - 2002/4/5. Y1 - 2002/4/5. UR - http://www.scopus.com/inward/record.url?scp=0037023755&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0037023755&partnerID=8YFLogxK. U2 - 10.1074/jbc.R100063200. DO - 10.1074/jbc.R100063200. M3 - Article. C2 - 11805123. AN - SCOPUS:0037023755. VL - 277. SP - 11617. EP - 11620. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 14. ER - ...
Define myeloproliferative: of, relating to, or being a disorder (such as leukemia) marked by excessive proliferation of bone marrow elements and…
Gentaur molecular products has all kinds of products like :search , Biotium \ CFDA SE CELL PROLIFERATION KIT \ 30050 for more molecular products just contact us
Hi, I wonder if somebody has already noted different results between the Promega MTS proliferation assay and the classical counting technique. Could you provide me a good, reliable, easy and fast technique to evaluate the cell proliferation. Thanks. Claude ...
MicroRNAs (miRNAs) are small noncoding, single-stranded RNAs consisting of 20-24 nucleotides (Bartel, Cell 116:281-297, 2004), which regulate target genes expression by interacting with...
Sigma-Aldrich offers abstracts and full-text articles by [A K Adams, G E Hallenbeck, K A Casper, Y J Patil, K M Wilson, R J Kimple, P F Lambert, D P Witte, W Xiao, M L Gillison, K A Wikenheiser-Brokamp, T M Wise-Draper, S I Wells].
Einleitung: Wachstumfaktor-assoziierte Proliferation von Zellen kann nach Stentimplantation eine Intimaverdickung im Lumen des Stents verursachen. Der perkutane Ersatz von Herzklappen mittels Transkathetertechnik ist eine neu entwickelte alternative Methode zur chirurgischen Implantation von prothetischen Herzklappen mit vielversprechenden Aussichten. Erste Langzeitdaten lassen vermuten, dass die Proliferation von Neointima einen negativen Einfluss auf die Funktion von klappentragenden Stents zeigt. Viele Studien demonstrieren den erfolgreichen Einsatz von immunsupprimierenden Medikamenten zur Senkung der neointimalen Proliferation in endovaskulären Stents. Die Zielsetzung dieser Studie war die Überprüfung der Frage, ob orales Everolimus inhibierend auf die neointimale Gewebsproliferation wirkt, welche mit der Implantation eines klappentragenden Stents in Pulmonalposition verbunden ist. Material und Methoden: In 12 Schweine wurden prothetische Herzklappen mittels einer transkutanen ...
PI-273 is a first reversibly and specific phosphatidylinositol 4-kinase (PI4KIIα) inhibitor with an IC50 of 0.47 μM. PI-273 can inhibit breast cancer cell proliferation, block the cell cycle and induce cell apoptosis. - Mechanism of Action & Protocol.
TY - JOUR. T1 - Naphthalene diimide-derivatives G-quadruplex ligands induce cell proliferation inhibition, mild telomeric dysfunction and cell cycle perturbation in U251MG glioma cells. AU - Muoio, Daniela. AU - Berardinelli, Francesco. AU - Leone, Stefano. AU - Coluzzi, Elisa. AU - di Masi, Alessandra. AU - Doria, Filippo. AU - Freccero, Mauro. AU - Sgura, Antonella. AU - Folini, Marco. AU - Antoccia, Antonio. N1 - © 2018 Federation of European Biochemical Societies.. PY - 2018/10. Y1 - 2018/10. N2 - In the present paper, the biological effects of three different naphthalene diimides (NDIs) G-quadruplex (G4) ligands (H-NDI-Tyr, H-NDI-NMe2, and tetra-NDI-NMe2) were comparatively evaluated to those exerted by RHPS4, a well-characterized telomeric G4-ligand, in an in vitro model of glioblastoma. Data indicated that NDIs were very effective in blocking cell proliferation at nanomolar concentrations, although displaying a lower specificity for telomere targeting compared to RHPS4. In addition, ...
TY - JOUR. T1 - Akt- and CREB-mediated prostate cancer cell proliferation inhibition by nexrutine, a Phellodendron amurense extract. AU - Garcia, Gretchen E.. AU - Nicole, Arevalo. AU - Bhaskaran, Shylesh. AU - Gupta, Ashima. AU - Kyprianouy, Natasha. AU - Kumar, Addanki P.. PY - 2006/1/1. Y1 - 2006/1/1. N2 - Evidence from epidemiological studies suggests that plant-based diets can reduce the risk of prostate cancer. However, very little information is available concerning the use of botanicals in preventing prostate cancer. As a first step toward developing botanicals as prostate cancer preventives, we examined the effect of Nexrutine on human prostate cancer cells. Nexrutine is a herbal extract developed from Phellodendron amurense. Phellodendron extracts have been used traditionally in Chinese medicine for hundreds of years as an anti-diarrheal, astringent, and anti-inflammatory agent. The present study investigated its potential antitumor effect on human prostate cancer cells.Our results ...
TY - JOUR. T1 - PTH-related protein enhances LoVo colon cancer cell proliferation, adhesion, and integrin expression. AU - Shen, Xiaoli. AU - Falzon, Miriam. PY - 2005/2/15. Y1 - 2005/2/15. N2 - Parathyroid hormone-related protein (PTHrP) has been localized in human colon cancer tissue and cell lines. Tumor cell adhesion to extracellular matrix (ECM) proteins plays a major role in the invasion and metastasis of tumor cells, and is mediated via integrin subunits. The LoVo human colon cancer cell line was used as a model system to study the effects of PTHrP on cell proliferation and adhesion to ECM proteins found in normal liver. Clones of LoVo cells engineered to overexpress PTHrP by stable transfection with a PTHrP cDNA showed enhanced cell proliferation vs. control (empty vector-transfected) cells. PTHrP-overexpressing cells also showed significantly higher adhesion to collagen type I, fibronectin, and laminin, and enhanced expression of the ∀2, ∀5, ∀6, ∃1 and ∃4 integrin subunits. ...
Nitric oxide (NO), which is known to inhibit systemic vascular smooth muscle cell proliferation, is used in the management of neonatal pulmonary hypertension. Our objectives were to determine: (1) if endogenous NO production by neonatal porcine pulmonary artery smooth muscle cells (PASMCs) varied with oxygen tension in vitro, and (2) the effect of exogenous NO and inducible NO synthase (iNOS) stimulators and inhibitors on PASMC proliferation and apoptosis. PASMCs were exposed to different conditions (varying PO2, NO donors and scavengers, iNOS stimulators and inhibitors) and proliferation, apoptosis, and cyclic guanosine 5-monophosphate (cGMP) assessed. PASMCs proliferated best between 5 and 10% O2 but cGMP levels were similar at all oxygen levels. NO donors (S-nitroso-N-acetyl-penicillamine, NOC-12, NOC-18) inhibited PASMC proliferation in a dose-dependent manner with associated cGMP increases, while NO scavengers (carboxy-PTIO), iNOS stimulators (interleukin-1β, lipopolysaccharide), and iNOS ...
TY - JOUR. T1 - Unusual roles of caspase-8 in triple-negative breast cancer cell line MDA-MB-231. AU - De Blasio, Anna. AU - Di Fiore, Riccardo. AU - Morreale, Marco. AU - Carlisi, Daniela. AU - Drago-Ferrante, Rosa. AU - Montalbano, Mauro. AU - Scerri, Christian. AU - Tesoriere, Giovanni. AU - Vento, Renza. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Triple-negative breast cancer (TNBC) is a clinically aggressive form of breast cancer that is unresponsive to endocrine agents or trastuzumab. TNBC accounts for ∼10-20% of all breast cancer cases and represents the form with the poorest prognosis. Patients with TNBC are at higher risk of early recurrence, mainly in the lungs, brain and soft tissue, therefore, there is an urgent need for new therapies. The present study was carried out in MDA-MB-231 cells, where we assessed the role of caspase-8 (casp-8), a critical effector of death receptors, also involved in non-apoptotic functions. Analysis of casp-8 mRNA and protein levels indicated that they were ...
Two estrogen receptors (ER), ERalpha and ERbeta, are expressed in breast cancer but their role in treatment response is unclear. The overall objective of this study was to determine if the presence of ERbeta protein in breast cancer cell lines is an indicator of a poor prognosis based on cell proliferation. In addition, we determined the effect of estradiol (E2) and selective estrogen receptor modulators (SERMs), such as tamoxifen and genistein, on ERalpha and ERbeta protein regulation, to help in the understanding of the mechanism behind their role in modulating cell proliferation. Using western blot and immunofluorescence analysis, the ER positive cell lines, MCF-7 and T47D, were found to contain both ERalpha and ERbeta, and thus were used as model systems. E2 and genistein, which increased cell proliferation in both cell lines, induced an up regulation of ERbeta in both cell lines. This suggests that an estrogenic response in breast cancer cells is indicated by an increase in ERbeta ...
Background: Dentigerous cyst is the most common type of developmental odontogenic cyst which originates from REE. It has the capacity for transformation to ameloblastoma, mucoepidermoid carcinoma and squamous cell carcinoma. ki-67 and PCNA are two cell proliferation markers which can be both detected in cysts and tumors of epithelial origin. ...
TY - JOUR. T1 - The role of B cell proliferation in the generation of immunoglobulin-secreting cells in man. AU - Jelinek, Diane F. AU - Lipsky, P. E.. PY - 1983. Y1 - 1983. N2 - The relationship of B cell proliferation and the generation of immunoglobulin-secreting cells (ISC) was explored in vitro by examining the effect of hydroxyurea (HU), an inhibitor of cellular DNA synthesis, on the generation of ISC from human peripheral blood mononuclear cells (PBM). HU completely inhibited the capacity of PBM to generate ISC in response to pokeweed mitogen (PWM) and other polyclonal B cell activators. Inhibition resulted from an effect on B cell proliferation, because HU also prevented the generation of ISC in cultures of purified B cells supplemented with either T cell supernatants or mitomycin C-treated T cells. Inhibiting B cell proliferation by treating them with mitomycin C before culture also abolished the generation of ISC. When ISC were enumerated after a 7-day incubation with PWM, the addition ...

Proliferation: MedlinePlus Medical EncyclopediaProliferation: MedlinePlus Medical Encyclopedia

Cancer cells are very prolific. They have high rates of cell division and growth. ... Proliferation is the growth of tissue cells. In many diseases, it is abnormal. ... Proliferation is the growth of tissue cells. In many diseases, it is abnormal. Cancer cells are very prolific. They have high ... Genetic control of protein synthesis, cell function, and cell reproduction. In: Hall JE, ed. Guyton and Hall Textbook of ...
more infohttps://medlineplus.gov/ency/article/002276.htm

Cell proliferation: MedlinePlus Medical Encyclopedia ImageCell proliferation: MedlinePlus Medical Encyclopedia Image

They have high rates of cell division and growth. ... Cancer cells are very prolific. They have high rates of cell ...
more infohttps://medlineplus.gov/ency/imagepages/9780.htm

Cell Proliferation AssaysCell Proliferation Assays

Cells are structural and functional unit of our body that control and maintain the function of all unicellular and ... Therefore, cell proliferation assays become crucial to scrutinise the rate of cell proliferation in both in vitro and in vivo ... in laboratories to determine cell proliferation. Key Concepts:. * Cell proliferation plays a vital role in regular tissue and ... by recycling the old cells with new cells. In addition, abnormal cell proliferation is also associated with various human ...
more infohttp://www.els.net/WileyCDA/ElsArticle/refId-a0002566.html

Cell Proliferation Assay KitsCell Proliferation Assay Kits

... and valuable tools for the quantitative evaluation of a cell populations response to external factors that affect cell ... Cell Proliferation Assay Kits ATCC Cell Proliferation Assay kits are convenient and valuable tools for the quantitative ... Is a resazurin-based cell proliferation assay that is sensitive and non-toxic. This single-component reagent can determine cell ... XTT Cell Proliferation Assay Kit Utilizes the second generation tetrazolium dye, XTT (sodium 2,3,-bis(2-methoxy-4-nitro-5- ...
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Cell Proliferation Assay KitsCell Proliferation Assay Kits

... and valuable tools for the quantitative evaluation of a cell populations response to external factors that affect cell ... Cell Proliferation Assay Kits ATCC Cell Proliferation Assay kits are convenient and valuable tools for the quantitative ... XTT Cell Proliferation Assay Kit Utilizes the second generation tetrazolium dye, XTT (sodium 2,3,-bis(2-methoxy-4-nitro-5- ... MTT Cell Proliferation Assay Kit Utilizes the most widely accepted detection reagents, tetrazolium salts, for the safe, ...
more infohttps://atcc.org/Products/Cells_and_Microorganisms/Testing_and_Characterization/Cell_Proliferation_Assay_Kits.aspx

Cell Proliferation Assay KitsCell Proliferation Assay Kits

... and valuable tools for the quantitative evaluation of a cell populations response to external factors that affect cell ... Cell Proliferation Assay Kits ATCC Cell Proliferation Assay kits are convenient and valuable tools for the quantitative ... Is a resazurin-based cell proliferation assay that is sensitive and non-toxic. This single-component reagent can determine cell ... XTT Cell Proliferation Assay Kit Utilizes the second generation tetrazolium dye, XTT (sodium 2,3,-bis(2-methoxy-4-nitro-5- ...
more infohttps://www.atcc.org/en/Products/Cells_and_Microorganisms/Testing_and_Characterization/Cell_Proliferation_Assay_Kits.aspx

Cell ProliferationCell Proliferation

... is an open-access journal devoted to studies into all aspects of cell proliferation and differentiation in ... Home , Journals , Cell Proliferation View PDF. Cell Proliferation. Editor(s): Dr. C. Sarraf. Publisher: Wiley. Impact Factor*: ... Cell Proliferation is an open-access journal devoted to studies into all aspects of cell proliferation and differentiation in ... In addition to original research papers Cell Proliferation publishes invited review articles, book reviews and letters ...
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Onion compound suppresses ovarian cancer cell proliferationOnion compound suppresses ovarian cancer cell proliferation

In addition, we found that ONA directly suppressed cancer cell proliferation. Thus, ONA is considered useful for the additional ... "Onion compound suppresses ovarian cancer cell proliferation." Medical News Today. MediLexicon, Intl., 22 Oct. 2016. Web.. 20 ... Ellis, M. (2016, October 22). "Onion compound suppresses ovarian cancer cell proliferation." Medical News Today. Retrieved from ... "We found that ONA reduced the extent of ovarian cancer cell proliferation induced by co-culture with human macrophages. ...
more infohttps://www.medicalnewstoday.com/articles/313643.php

Recombinant DNA And Cell Proliferation - 1st EditionRecombinant DNA And Cell Proliferation - 1st Edition

Purchase Recombinant DNA And Cell Proliferation - 1st Edition. Print Book & E-Book. ISBN 9780126650808, 9780323153362 ... Murine Erythroleukemia Cells (Friend Cells). III. Role of the Cell Cycle in Friend Cell Differentiation. IV. Friend Cell ... SV40 and Cell Proliferation. IV. A Growing Cell Receives Signals for Growth in Size. V. A Growing Cell Receives Signals to ... Mathematical Models and Kinetics of Cell Proliferation. III. Biochemistry of Cell Proliferation. IV. The Role of RNA Polymerase ...
more infohttps://www.elsevier.com/books/recombinant-dna-and-cell-proliferation/stein/978-0-12-665080-8

Ions, Cell Proliferation, and Cancer - 1st EditionIons, Cell Proliferation, and Cancer - 1st Edition

Cell Proliferation, and Cancer - 1st Edition. Print Book & E-Book. ISBN 9780121230500, 9781483277486 ... Ions, Cell Proliferation, and Cancer present the credibility of ions as specific regulators of cell proliferation. This book ... Section III: Divalent Ions, Cell Proliferation, and Cancer. The Roles of Calcium and Magnesium in Cell Proliferation: An ... Section II: Monovalent Ions, Cell Proliferation, and Cancer. Monovalent Cations, Cell Proliferation, and Cancer: An Overview. ...
more infohttps://www.elsevier.com/books/ions-cell-proliferation-and-cancer/boynton/978-0-12-123050-0

Promoting Pancreatic Cell Proliferation | Science SignalingPromoting Pancreatic Cell Proliferation | Science Signaling

... whereas β cell-specific overexpression of Smad7 enhanced proliferation of β cells and expression of CyclinD1 and CyclinD2. M2 ... β cell-specific deficiency of Smad7 did not affect macrophage infiltration but reduced β cell proliferation and expression of ... reduced pancreatic infiltration of macrophages and proliferation of β cells induced by PDL. Isolated β cells of control but not ... Some preclinical therapeutic strategies have focused on β cell replacement through differentiation of progenitor cells; however ...
more infohttps://stke.sciencemag.org/content/7/320/ec94.abstract

Cell Proliferation Assays (         Abstract          The cel...)Cell Proliferation Assays ( Abstract The cel...)

The cell proliferation assay is an important tool in the assessment of... Introduction ...Cell proliferation assays are ... employed frequently in immunological ca...,Cell,Proliferation,Assays,biological,advanced biology technology,biology laboratory ... Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000. 7. Fluorescence- ... Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted. 2. Quantitative Measurement of Cell ...
more infohttp://www.bio-medicine.org/biology-technology/Cell-Proliferation-Assays-1414-1/

ISL1 Promotes Pancreatic Islet Cell ProliferationISL1 Promotes Pancreatic Islet Cell Proliferation

Using HIT-T15 and primary adult islet cells as cell models, we show that ISL1 promoted adult pancreatic islet cell ... Conclusions/Significance ISL1 promoted adult pancreatic islet cell proliferation and probably by activating c-Myc and CyclinD1 ... while knockdown of ISL1 increased the proportion of cells in G1 phase and decreased the proportion of cells in G2/M and S ... Our findings extend the knowledge about the crucial role of ISL1 in maintaining mature islet cells homeostasis. Our results ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0022387

Phosphoinositide metabolism and control of cell proliferation | SpringerLinkPhosphoinositide metabolism and control of cell proliferation | SpringerLink

... the essential control of cell proliferation is therefore the signal that commits a cell to... ... In most cells initiation of DNA synthesis is inexorably followed by a premitotic phase and then by mitosis, ... the essential control of cell proliferation is therefore the signal that commits a cell to DNA synthesis. Since cells may be ... Phosphoinositide metabolism and control of cell proliferation. In: Miller A.O.A. (eds) Advanced Research on Animal Cell ...
more infohttps://link.springer.com/chapter/10.1007/978-94-009-0875-8_13

NIOSHTIC-2  Publications Search - 20030997 - Strawberries inhibit cancer cell proliferation.NIOSHTIC-2 Publications Search - 20030997 - Strawberries inhibit cancer cell proliferation.

... and inhibitory effect on proliferation of A549 human lung epithelial cancer cells. Differences among the strawberry genotypes ... between the scavenging capacities for the reactive oxygen species and the inhibition of cancer cell proliferation were 0.8074, ... There was also a relationship between scavenging capacity and the inhibition of cancer cell proliferation. The correlations (R2 ... and inhibitory effect on proliferation of A549 human lung epithelial cancer cells. Differences among the strawberry genotypes ...
more infohttps://www.cdc.gov/niosh/nioshtic-2/20030997.html

cell proliferation | Gutcell proliferation | Gut

Label-retaining liver cancer cells are relatively resistant to sorafenib Hong-Wu Xin, Chenwi M Ambe, Danielle M Hari, Gordon W ... TGR5 is essential for bile acid-dependent cholangiocyte proliferation in vivo and in vitro Maria Reich, Kathleen Deutschmann, ... Hepatitis delta virus persists during liver regeneration and is amplified through cell division both in vitro and in vivo Katja ... Helicobacter pylori targets cancer-associated apical-junctional constituents in gastroids and gastric epithelial cells Lydia E ...
more infohttps://gut.bmj.com/keyword/cell-proliferation

PROGRAM FOR EPITHELIAL CELL PROLIFERATION | SBIR.govPROGRAM FOR EPITHELIAL CELL PROLIFERATION | SBIR.gov

THIS PROJECT WILL DEVELOP A COMPUTER PROGRAM FOR FUNCTIONAL ASSESSMENT OF EPITHELIAL TISSUE IN THE GASTROINTESTINAL (GI)TRACT. THE COMPUTER PROGRAM RELATING IMAGE ANALYSIS TO EPITHELIAL TISSUE KINETICS AND REGENERATION WILL BE IMPLEMENTED ON THE EXISTING LOATS ASSOCIATES, INC., PORTABLE COMPUTER ENDOSCOPIC IMAGE ACQUISITION STATION ON WHICH ULCERS CAN BE VIEWED IN REAL TIME AND IN TRUE COLOR BY RESEARCHERS AND CLINICIANS AS A CONSEQUENCE OF A NORMAL GI EXAMINATION. SPECIFIC ALGORITHMS WILL BE DEVELOPED THAT WILL PROVIDE QUANTITATIVE INFORMATION ON: (1) ULCER-HEALING KINETICS, AREA, AND AREA PROPERTIES; (2) FOURIER SHAPE CHARACTERISTICS; (3) COLOR AND TEXTURE MEASUREMENTS; AND (4)SURFACE DEPTH. A STUDY OF RESULTS WILL BE CONDUCTED THAT WILL CHARACTERIZE THE KINETICS AND REGENERATION RATES OF EPITHELIAL TISSUE AS MEASURED IN THE ULCER-HEALING PROCESS. PHASE II WILL INCLUDE THE EFFECTS OF EXOGENOUS AGENTS, ACCOMMODATION OF AN EXPANDED RANGE OF IMAGE ACQUISITION MODALITIES, AND PRODUCTION ...
more infohttps://www.sbir.gov/sbirsearch/detail/217701

The C60(FeCp2)2-Based Cell Proliferation AcceleratorThe C60(FeCp2)2-Based Cell Proliferation Accelerator

Figure 2: The rin-mf5 cell proliferation in presence of the C60(FeCp2)2 particles. (a) Cell proliferation (standard condition ... c) Influence of the crystals with size of nearly 5 μm on the cell proliferation. (d) Cell proliferation in presence of the C60( ... In order to study the influence of the fulleride presence on cell proliferation, we chose the cell line rin-mf5 (cell ... The C60(FeCp2)2-Based Cell Proliferation Accelerator. Andrei Soldatov,1 Edward Shpilevsky,2 Vitaliy Goranov,3 Vladimir ...
more infohttps://www.hindawi.com/journals/jchem/2013/840614/

β-Lactoglobulin Influences Human Immunity and Promotes Cell Proliferationβ-Lactoglobulin Influences Human Immunity and Promotes Cell Proliferation

... Chun San Tai,1,2 Yi Yun Chen,2 and Wen Liang Chen1 ... Chun San Tai, Yi Yun Chen, and Wen Liang Chen, "β-Lactoglobulin Influences Human Immunity and Promotes Cell Proliferation," ...
more infohttps://www.hindawi.com/journals/bmri/2016/7123587/cta/

Oncogenes, Inositol Lipids & Cell Proliferation | Cancer Council VictoriaOncogenes, Inositol Lipids & Cell Proliferation | Cancer Council Victoria

Oncogenes, Inositol Lipids and Cell Proliferation Lead researcher. Dr J A Hamilton ... Cancer is a disease of the cells, which are the bodys basic building blocks. ...
more infohttps://www.cancervic.org.au/research/projects/project_oncogenes_inositol_lip.html

Cell Proliferation And The Control Of Cellular Function | BartlebyCell Proliferation And The Control Of Cellular Function | Bartleby

Nicotine Cell Proliferation And Invasion. 1807 Words , 8 Pages. induces cell proliferation and invasion as well as epithelial ... Animal Cells And Their Functions. 1044 Words , 5 Pages. Animal cells and their functions Cell Membrane The cell "surface" ... Eukaryotic Cells And Its Functions. 978 Words , 4 Pages. *. The Effect Of Calf Serum On Cell Proliferation. 1154 Words , 5 ... More about Cell Proliferation And The Control Of Cellular Function. *. Cell Cycle And Dna Rn Mirnas Regulating Cellular ...
more infohttps://www.bartleby.com/essay/Cell-Proliferation-And-The-Control-Of-Cellular-F322Q4L2LBQQ

Medical Xpress - cell proliferationMedical Xpress - cell proliferation

Cell growth. The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in ... it refers to growth of cell populations, where one cell (the "mother cell") grows and divides to produce two "daughter cells". ... Related topics: cancer cells · breast cancer · cancer · tumor growth · stem cells Sorry, no news articles match your request. ...
more infohttps://medicalxpress.com/tags/cell+proliferation/sort/date/6h/

FSTL3: A Crucial Regulator of Sertoli Cell ProliferationFSTL3: A Crucial Regulator of Sertoli Cell Proliferation

Increased cellular proliferation in KO testes as shown by increased proliferating cell nuclear antigen (PCNA) staining and ... FSTL3: A Crucial Regulator of Sertoli Cell Proliferation. Notwithstanding current world population, infertility in humans is on ... Sertoli cells (SC) in the testis allow for the duplications and development of the cells that give rise to sperm and generally ... Also, within the testis there is an increase in SC numbers and related increase in cells that give rise to sperm. We, therefore ...
more infohttps://www.rvc.ac.uk/research/about/animals-in-research/case-studies/fstl3-a-crucial-regulator-of-sertoli-cell-proliferation

IL-6 drives T cell proliferation | JEMIL-6 drives T cell proliferation | JEM

... the gp130 mutation caused nonhematopoietic cells to produce excess IL-7-a growth factor that triggers T cell proliferation. ... T cells or antibody-producing B cells.. The CD4+ cells did not appear to cause disease because of an affinity for joint- ... Rather, the cells simply proliferated excessively in the mutant mice. This hyperproliferation was not the fault of the T cell, ... might exacerbate disease by inducing IL-7 and thus driving T cell activation. What causes the overstimulated T cells to attack ...
more infohttp://jem.rupress.org/content/203/6/1387b

BioTek Applications: Cell ProliferationBioTek Applications: Cell Proliferation

Gen5 allows measuring cell proliferation as both an end point and kinetic imaging assay and can be run with temperature and gas ... Kinetic live cell proliferation assays using the Lionheart™ FX or the BioSpa™ 8 Automated Incubator and Cytation™ Cell Imaging ... Kinetic Proliferation Assay Using Label-Free Cell Counting *Monitoring Saccharomyces cerevisiea Growth with Brightfield ... Characterizing cell proliferation is a crucial aspect of biological research and therapeutic drug development. Most current ...
more infohttps://www.biotek.com/applications/cell-proliferation.html
  • The process of cell proliferation and differentiation plays a vital role from the time of embryogenesis to development of whole organism from single‐ or double‐cell embryo and continues its crucial role in maintenance of adult tissue homoeostasis by recycling the old cells with new cells. (els.net)
  • Topics include gene transfer for assessing the role of defined DNA sequences in triggering DNA replication, nucleic acid hybridization probes for analyzing the regulation of specific genes during the cell cycle, and cloned DNAs for studying genes expressed with proliferation and differentiation. (elsevier.com)
  • This book also introduces the reader to the role of the cell division cycle in induced differentiation, gene regulation in muscle cells, regulation of nonmuscle actin gene expression during early development, and sequences at ends of cellular DNA molecules in relation to telomere replication and function. (elsevier.com)
  • They also discovered that ONA inhibited pro-tumor activities of myeloid-derived suppressor cells (MDSC), which the researchers say are linked with the suppression of the anti-tumor immune response of host lymphocytes. (medicalnewstoday.com)
  • Brief scheme of metabolic activity measurement using MTT and other tetrazolium salts to access cell proliferation. (els.net)
  • Barltrop J and Owen T (1991) 5‐(3‐carboxymethoxyphenyl)‐2‐(4,5‐dimethylthiazoly)‐3‐(4‐sulfophenyl) tetrazolium, inner salt (MTS) and related analogs of 3‐(4,5‐dimethylthiazolyl)‐2,5‐diphenyltetrazolium bromide (MTT) reducing to purple water‐soluble formazans as cellviability indicators. (els.net)
  • Berridge MV, Herst PM and Tan AS (2005) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. (els.net)
  • Utilizes the most widely accepted detection reagents, tetrazolium salts, for the safe, accurate, and straightforward quantification of changes in cell proliferation. (atcc.org)
  • M2 macrophages isolated from the pancreas of mice subjected to PDL showed greater expression of TGFβ1 , which encodes a ligand for TGFβ receptors, and chemical inhibition of type I TGFβ receptors inhibited proliferation of β cells cocultured with M2 macrophages. (sciencemag.org)
  • When neurotransmitters bind to receptors in the membranes of certain cells, they elicit a direct response within the cell. (news-medical.net)
  • They found not only that the stem cells did have receptors for ANS neurotransmitters, but also the neurotransmitters changed the behavior of the cells - just what they would expect to see for a direct relationship. (news-medical.net)
  • When we isolated the stem cells and found there were actually ANS neurotransmitter receptors, we found that missing piece,' Davis says. (news-medical.net)
  • Thus, during pancreatic injury, macrophages release TGFβ1 to stimulate Smad7 expression in β cells, which in turn inhibits an inhibitor of cell cycle progression and promotes β cell expansion. (sciencemag.org)
  • Nevertheless two general problems emerge that we must address in seeking to understand how mitogens stimulate cells to proliferate. (springer.com)
  • Fiorito and coauthors showed that C-fullerenes, when highly purified, do not stimulate the release of NO by murine macrophage cells in culture, their uptake by human macrophage cells is very low, and they possess a very low toxicity against human macrophage cells [ 11 ]. (hindawi.com)
  • This text also deals with topics such as the use of cloned SV40 DNA fragments to examine signals for cell proliferation, expression of dihydrofolate reductase and thymidylate synthase genes in mammalian cells, and gene expression during the cell cycle of Chlamydomonas reinhardtii. (elsevier.com)
  • Scheme of labelled nucleotide analogue incorporation during DNA replication to measure cell proliferation rate. (els.net)
  • laboratory of DNA Replication and Cell Cycle at National Institute of Immunology, India, under the supervision of Dr. Sandeep Saxena. (bartleby.com)
  • The main focus of the lab is to study the role of non-coding RNAs in regulation of Cell Cycle and DNA replication and also to provide mechanical insights in understanding the checkpoint response to aberrations in replication complexes. (bartleby.com)
  • Prior to Davis's study, which is published in Physiological Reports , scientists had suspected the ANS was involved in stem cell proliferation, but they didn't know if the relationship was direct or indirect. (news-medical.net)
  • When we simulated activation of either of those systems, we saw a decrease in stem cell proliferation,' Dailey says. (news-medical.net)
  • Although the research focused on the intestinal epithelium, Davis and Dailey suspect the ANS is directly controlling stem cell proliferation in other parts of the body, as well. (news-medical.net)
  • Puri is the author of over 300 archival and conference publications, reports, and book chapters in the fields of combustion, energy and transport phenomena, thermomagnetic convection and magnetic fluid transport, and the cancer stem cell hypothesis. (wikipedia.org)
  • The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
  • The researchers say their study demonstrates that ONA slows progression of ovarian cancer tumors by interrupting myeloid cells' pro-tumor activity. (medicalnewstoday.com)
  • The expression, response, signaling, and survival of normal human cells allows for the progression of mankind. (bartleby.com)
  • 1999). Moreover, HSP-70 might play important roles in survival and proliferation of pathogens, such as Schistosoma, Plasmodium, Trypanosoma, and Leishmania within the host (Lindquist, 1986). (bartleby.com)
  • The nuclear abundance of the cyclin-dependent kinase inhibitor 1β (also known as p27) was decreased in β cells from mice subjected to PDL, those overexpressing Smad7, or those cocultured with M2 macrophages. (sciencemag.org)
  • Our findings in genetically modified mice lacking FSTL3 in all cells show increased testis size, and a lack of testis size reduction with age. (rvc.ac.uk)
  • The authors now show that disease development in these mice depends on CD4 + T cells, but not on cytolytic CD8 + T cells or antibody-producing B cells. (rupress.org)
  • Rather, the cells simply proliferated excessively in the mutant mice. (rupress.org)
  • This hyperproliferation was not the fault of the T cell, as wild-type CD4 + T cells also multiplied excessively and caused disease when transferred into irradiated mutant mice. (rupress.org)
  • To characterize the relationship, the researchers focused on stem cells in the intestinal lining, or epithelium, in mice. (news-medical.net)
  • The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
  • The influence of fulleride particles on the cell proliferative activity was also investigated. (hindawi.com)
  • We found that the proliferative activity of the RINmF5 cells increases (53% versus control) in presence of the C 60 (FeCp 2 ) 2 nanosized particles. (hindawi.com)
  • We have found that a natural cell product, follistatin-like 3 (FSTL3) might be crucial in regulating testicular development. (rvc.ac.uk)
  • induces cell proliferation and invasion as well as epithelial to mesenchymal transition (EMT), even though nicotine is not carcinogenic on its own. (bartleby.com)
  • Water-soluble fullerene derivatives protect human keratinocytes from UV-induced cell injuries together with the decreases in intracellular ROS generation and DNA damages [ 12 ] and suppress intracellular lipid accumulation [ 13 ]. (hindawi.com)
  • Sertoli cells (SC) in the testis allow for the duplications and development of the cells that give rise to sperm and generally the total number of germ cells produced depends on the number of SC in a testis. (rvc.ac.uk)
  • This finding often create alarm and anxiety, because it has to be placed in a differential diagnosis versus low-stage malignant germ cell tumors. (clinicaltrials.gov)
  • Patients with the testicular dysgenesis syndrome, that comprises a variable spectrum of clinical manifestations, such as infertility, cryptorchidism, hypospadias, impaired spermatogenesis and testicular germ cell neoplasms, often develop alterations in the Leydig cell compartment. (clinicaltrials.gov)
  • Leydig cell tumors (LCTs), although uncommon in the general population, are the most frequent non-germ cell testicular neoplasms, and their incidence has been reported increasingly growing, especially in infertile patients. (clinicaltrials.gov)
  • Given that the focal areas of Leydig cell hyperplasia are nowadays easily detectable at ultrasonography of the testis (US), as small non-palpable hypoechoic micro-nodules that can show internal vascularization, their finding create a diagnostic challenge versus low-stage malignant germ cell tumors. (clinicaltrials.gov)
  • Organized into three sections encompassing 32 chapters, this book begins with an overview of the important role that ions in animal cells play in a variety of fundamental processes associated with essential cell functions. (elsevier.com)
  • All of the processes involved in increasing Cell number including Cell division . (jove.com)
  • Nevertheless, explorative surgery reveal that a consistent number of these lesion are benign, due to Leydig cell hyperplasia or Leydig cell tumours. (clinicaltrials.gov)
  • Is the Subject Area "Cell cycle and cell division" applicable to this article? (plos.org)
  • IL-6 has been implicated in RA and other T cell-driven autoimmune diseases. (rupress.org)
  • This book is organized into three sections encompassing 13 chapters and begins with a discussion on the expression of specific genes during the cell cycle. (elsevier.com)
  • Recombinant DNA and Cell Proliferation focuses on the use of recombinant DNA technology in investigating the regulation of cell proliferation. (elsevier.com)