All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A cell line derived from cultured tumor cells.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Established cell cultures that have the potential to propagate indefinitely.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Protein encoded by the bcl-1 gene which plays a critical role in regulating the cell cycle. Overexpression of cyclin D1 is the result of bcl-1 rearrangement, a t(11;14) translocation, and is implicated in various neoplasms.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Elements of limited time intervals, contributing to particular results or situations.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
The nonstriated involuntary muscle tissue of blood vessels.
A cyclin-dependent kinase inhibitor that coordinates the activation of CYCLIN and CYCLIN-DEPENDENT KINASES during the CELL CYCLE. It interacts with active CYCLIN D complexed to CYCLIN-DEPENDENT KINASE 4 in proliferating cells, while in arrested cells it binds and inhibits CYCLIN E complexed to CYCLIN-DEPENDENT KINASE 2.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Endogenous or exogenous substances which inhibit the normal growth of human and animal cells or micro-organisms, as distinguished from those affecting plant growth (= PLANT GROWTH REGULATORS).
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A mitogen-activated protein kinase subfamily that is widely expressed and plays a role in regulation of MEIOSIS; MITOSIS; and post mitotic functions in differentiated cells. The extracellular signal regulated MAP kinases are regulated by a broad variety of CELL SURFACE RECEPTORS and can be activated by certain CARCINOGENS.
Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.
A cyclin-dependent kinase inhibitor that mediates TUMOR SUPPRESSOR PROTEIN P53-dependent CELL CYCLE arrest. p21 interacts with a range of CYCLIN-DEPENDENT KINASES and associates with PROLIFERATING CELL NUCLEAR ANTIGEN and CASPASE 3.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.
The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.
Tumors or cancer of the human BREAST.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Adherence of cells to surfaces or to other cells.
Signal molecules that are involved in the control of cell growth and differentiation.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
Proteins prepared by recombinant DNA technology.
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
An expression of the number of mitoses found in a stated number of cells.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Restoration of integrity to traumatized tissue.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from
Formation of NEURONS which involves the differentiation and division of STEM CELLS in which one or both of the daughter cells become neurons.
The physiological renewal, repair, or replacement of tissue.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Methods for maintaining or growing CELLS in vitro.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Tumors or cancer of the COLON.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Transplantation between animals of different species.
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Tumors or cancer of the PROSTATE.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
Regulatory signaling systems that control the progression of the CELL CYCLE through the G1 PHASE and allow transition to S PHASE when the cells are ready to undergo DNA REPLICATION. DNA DAMAGE, or the deficiencies in specific cellular components or nutrients may cause the cells to halt before progressing through G1 phase.
Antibodies produced by a single clone of cells.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
A serine threonine kinase that controls a wide range of growth-related cellular processes. The protein is referred to as the target of RAPAMYCIN due to the discovery that SIROLIMUS (commonly known as rapamycin) forms an inhibitory complex with TACROLIMUS BINDING PROTEIN 1A that blocks the action of its enzymatic activity.
Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events.
A quiescent state of cells during G1 PHASE.
A 50-kDa protein that complexes with CYCLIN-DEPENDENT KINASE 2 in the late G1 phase of the cell cycle.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
The action of a drug in promoting or enhancing the effectiveness of another drug.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Transport proteins that carry specific substances in the blood or across cell membranes.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
Agents that inhibit PROTEIN KINASES.
A cyclin subtype that is specific for CYCLIN-DEPENDENT KINASE 4 and CYCLIN-DEPENDENT KINASE 6. Unlike most cyclins, cyclin D expression is not cyclical, but rather it is expressed in response to proliferative signals. Cyclin D may therefore play a role in cellular responses to mitogenic signals.
An encapsulated lymphatic organ through which venous blood filters.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Cellular DNA-binding proteins encoded by the sis gene (GENES, SIS). c-sis proteins make up the B chain of PLATELET-DERIVED GROWTH FACTOR. Overexpression of c-sis causes tumorigenesis.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A cyclin D subtype which is regulated by GATA4 TRANSCRIPTION FACTOR. Experiments using KNOCKOUT MICE suggest a role for cyclin D2 in granulosa cell proliferation and gonadal development.
The thin membranous structure supporting the adjoining glomerular capillaries. It is composed of GLOMERULAR MESANGIAL CELLS and their EXTRACELLULAR MATRIX.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Progenitor cells from which all blood cells derive.
Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
The process by which a DNA molecule is duplicated.
Tumors or cancer of the LIVER.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
Repair or renewal of hepatic tissue.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
The rate dynamics in chemical or physical systems.
Mode of communication wherein a bound hormone affects the function of the cell type that produced the hormone.
A cyclin subtype that has specificity for CDC2 PROTEIN KINASE and CYCLIN-DEPENDENT KINASE 2. It plays a role in progression of the CELL CYCLE through G1/S and G2/M phase transitions.
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A key regulator of CELL CYCLE progression. It partners with CYCLIN E to regulate entry into S PHASE and also interacts with CYCLIN A to phosphorylate RETINOBLASTOMA PROTEIN. Its activity is inhibited by CYCLIN-DEPENDENT KINASE INHIBITOR P27 and CYCLIN-DEPENDENT KINASE INHIBITOR P21.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

Emerging targets: molecular mechanisms of cell contact-mediated growth control. (1/45748)

Contact inhibition of cell proliferation evokes a unique cellular program of growth arrest compared with stress, age, or other physical constraints. The last decade of research on genes activated by cell-cell contact has uncovered features of transmembrane signaling, cytoskeletal reorganization, and transcriptional control that initiate and maintain a quiescent phenotype. This review will focus on mechanisms controlling contact inhibition of cell proliferation, highlighting specific gene expression responses that are activated by cell-cell contact. Although a temporal framework for imposition of these mechanisms has not yet been well described, contact inhibition of cell proliferation clearly requires their coordinated function. Novel targets for intervention in proliferative disorders are emerging from these studies.  (+info)

Cell cycle dysregulation in oral cancer. (2/45748)

The dysregulation of the molecular events governing cell cycle control is emerging as a central theme of oral carcinogenesis. Regulatory pathways responding to extracellular signaling or intracellular stress and DNA damage converge on the cell cycle apparatus. Abrogation of mitogenic and anti-mitogenic response regulatory proteins, such as the retinoblastoma tumor suppressor protein (pRB), cyclin D1, cyclin-dependent kinase (CDK) 6, and CDK inhibitors (p21(WAF1/CIP1), p27(KIP1), and p16(INK4a)), occur frequently in human oral cancers. Cellular responses to metabolic stress or genomic damage through p53 and related pathways that block cell cycle progression are also altered during oral carcinogenesis. In addition, new pathways and cell cycle regulatory proteins, such as p12(DOC-1), are being discovered. The multistep process of oral carcinogenesis likely involves functional alteration of cell cycle regulatory members combined with escape from cellular senescence and apoptotic signaling pathways. Detailing the molecular alterations and understanding the functional consequences of the dysregulation of the cell cycle apparatus in the malignant oral keratinocyte will uncover novel diagnostic and therapeutic approaches.  (+info)

Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. (3/45748)

BACKGROUND: The Hedgehog (Hh) signaling pathway is vital to animal development as it mediates the differentiation of multiple cell types during embryogenesis. In adults, Hh signaling can be activated to facilitate tissue maintenance and repair. Moreover, stimulation of the Hh pathway has shown therapeutic efficacy in models of neuropathy. The underlying mechanisms of Hh signal transduction remain obscure, however: little is known about the communication between the pathway suppressor Patched (Ptc), a multipass transmembrane protein that directly binds Hh, and the pathway activator Smoothened (Smo), a protein that is related to G-protein-coupled receptors and is capable of constitutive activation in the absence of Ptc. RESULTS: We have identified and characterized a synthetic non-peptidyl small molecule, Hh-Ag, that acts as an agonist of the Hh pathway. This Hh agonist promotes cell-type-specific proliferation and concentration-dependent differentiation in vitro, while in utero it rescues aspects of the Hh-signaling defect in Sonic hedgehog-null, but not Smo-null, mouse embryos. Biochemical studies with Hh-Ag, the Hh-signaling antagonist cyclopamine, and a novel Hh-signaling inhibitor Cur61414, reveal that the action of all these compounds is independent of Hh-protein ligand and of the Hh receptor Ptc, as each binds directly to Smo. CONCLUSIONS: Smo can have its activity modulated directly by synthetic small molecules. These studies raise the possibility that Hh signaling may be regulated by endogenous small molecules in vivo and provide potent compounds with which to test the therapeutic value of activating the Hh-signaling pathway in the treatment of traumatic and chronic degenerative conditions.  (+info)

Gleevec (STI-571) inhibits lung cancer cell growth (A549) and potentiates the cisplatin effect in vitro. (4/45748)

BACKGROUND: Gleevec (aka STI571, Imatinib) is a recently FDA approved anti-tumor drug for chronic myelogenous leukemia. Gleevec binds specifically to BCR-ABL tyrosine kinase and inhibit the tyrosine kinase activity. It cross-reacts with another two important membrane tyrosine kinase receptors, c-kit and PDGF receptors. We sought to investigate if Gleevec has a potential role in treatment of non-small cell lung cancer. RESULTS: We have shown that Gleevec alone can inhibit the A549 lung cancer cell growth in dose-dependent manner, and the optimal concentration of Gleevec inhibition of A549 cell growth is at the range of 2-3 microM (IC50). We have also shown that A549 cells are resistant to cisplatin treatment (IC50 64 microM). Addition of Gleevec to the A549 cells treated with cisplatin resulted in a synergistic cell killing effect, suggesting that Gleevec can potentiate the effect of cisplatin on A549 cells. We also showed that the A549 lung cancer cells expresses the platelet derived growth factor receptor alpha, and the inhibitory effects of Gleevec on A549 cells is likely mediated through inhibition of PDGFR alpha phosphorylation. We further tested 33 lung cancer patients' tumor specimens to see the frequency of PDGFR-alpha expression by tissue micro-arrays and immunohistochemistry. We found that 16 of the 18 squamous carcinomas (89%), 11 of the 11 adenocarcinomas (100%), and 4 of the 4 small cell lung cancers (100%) expressed PDGFR-alpha. CONCLUSION: These results suggest a potential role of Gleevec as adjuvant therapeutic agent for treatment of non-small cell lung cancer.  (+info)

Hepatocarcinogenic potential of the glucocorticoid antagonist RU486 in B6C3F1 mice: effect on apoptosis, expression of oncogenes and the tumor suppressor gene p53. (5/45748)

BACKGROUND: Glucocorticoids inhibit hepatocellular proliferation and modulate the expression of oncogenes and tumor suppressor genes via mechanisms involving the glucocorticoid receptor. Glucocorticoids also produce a receptor-mediated inhibitory effect on both basal and hormone-stimulated expression of a newly discovered family of molecules important for shutting off cytokine action. We therefore hypothesized that inhibiting glucocorticoid receptors may disturb hepatocellular growth and apoptosis. Consequently, we investigated the effect of RU486, a potent antagonist of the glucocorticoid receptor, on basal levels of hepatocellular proliferation and apoptosis in male B6C3F1 mice. Furthermore, we evaluated the effect of this compound on cellular genes involved in the regulation of these important processes. RESULTS: Data show that treatment of male B6F3C1 mice with RU486 (2 mg/kg/d, ip) for 7 days dramatically inhibited liver cell proliferation by about 45% and programmed hepatocellular death by approximately 66%. RU 486 also significantly increased hepatic expression of the oncogenes mdm2 and JunB, while reducing that of the tumor suppressor gene p53. CONCLUSION: Exposure to RU486 may ultimately enhance the susceptibility of the liver to cancer risk by diminishing its ability to purge itself of pre-cancerous cells via apoptosis. This effect may be mediated through increases in the hepatic expression of the oncogene mdm2, coupled with decreases in that of the tumor suppressor gene p53. The decrease in hepatocellular proliferation caused by RU 486 may be related to effects other than its anti-glucocorticoid activity.  (+info)

Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat. (6/45748)

The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on proliferation of vascular smooth muscle cells (VSMCs) stimulated by advanced glycation end products (AGE) and neointima hyperplasia after rat carotid arterial injury. Rat aortic VSMCs were cultured and treated with rhIL-10 or AGE respectively, and then co-treated with rhIL-10 and AGE. Proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytomertry. Sprague-Dawley rats were treated with recombinant human IL-10 (rhIL-10) for 3 d after carotid arteries injury. The ratio of neointima to media area at the site of arterial injury was measured 28 d after balloon injury. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control, AGE stimulated VSMCs proliferation. rhIL-10 alone had no effect on VSMCs growth. With AGE stimulation, rhIL-10, at dose as low as 10 ng/ml, inhibited VSMCs growth (P<0.05). The cell number in G(0)/G(1) phase of AGE and rhIL-10 co-treatment group was higher than that of AGE treatment alone (P<0.01) by flow cytometry analysis. Compared with the control group of neointima hyperplasia in rats, the ratio of neointima to media area of recombinant human IL-10 group was reduced by 45% (P<0.01). The p44/42 MAPK activity was significantly enhanced by AGE. The AGE effects were opposed by rhIL-10. The anti-inflammatory cytokine rhIL-10 inhibits AGE-induced VSMCs proliferation. Recombinant human IL-10 also inhibited neointima hyperplasia after carotid artery injury in rats. The results suggest the possibility that recombinant human IL-10, as a potential therapeutic approach, prevents neointimal hyperplasia.  (+info)

The effects of inhibiting P18(INK4C) expression on the invasion of gastric adenocarcinoma cell line. (7/45748)

Using cDNA microarray with double dots of 4096 human genes, P18(INK4C), a member of CKI, was found down-regulated in a gastric adenocarcinoma metastatic cell line (RF-48), compared with the corresponding primary cancer cell line (RF-1), which implied that P18(INK4C) might be involved in cell invasion and metastatic progression of human gastric adenocarcinoma. Antisense RNA expression plasmid was applied to inhibit P18(INK4C) expression to study the effect of decreased P18(INK4C) expression on cell migration, invasion and proliferation ability and cell cycle of RF-1. Results showed that inhibition of P18(INK4C) expression could obviously enhance cell invasion ability of RF-1, but had little effect on its cell cycle and cell migration and proliferation ability. These results implied that P18(INK4C) might play a pivotal role in regulating cell invasion, rather than regulating cell cycle and proliferation in the progression of human gastric adenocarcinoma as expected before.  (+info)

Comparative proteomic analysis of proliferating and functionally differentiated mammary epithelial cells. (8/45748)

Proliferation and differentiation of mammary epithelial cells are governed by hormonal stimuli, cell-cell, and cell-matrix interactions. Terminal differentiation of mammary epithelial cells depends upon the action of the lactogenic hormones, insulin, glucocorticoids, and prolactin that enable them to synthesize and secrete milk proteins. These differentiated cells are polarized and carry out vectorial transport of milk constituents across the apical plasma membrane. To gain additional insights into the mechanisms governing differentiation of mammary epithelial cells, we identified proteins whose expression distinguishes proliferating from differentiated mammary epithelial cells. For this purpose we made use of the HC11 mammary epithelial line, which is capable of differentiation in response to lactogenic hormones. Using two-dimensional gel electrophoresis and mass spectrometry, we found about 60 proteins whose expression levels changed in between these two differentiation states. Bioinformatic analysis revealed differential expression of cytoskeletal components, molecular chaperones and regulators of protein folding and stability, calcium-binding proteins, and components of RNA-processing pathways. The actin cytoskeleton is asymmetrically distributed in differentiated epithelial cells, and the identification of proteins involved in mRNA binding and localization suggests that asymmetry might in part be achieved by controlling cellular localization of mRNAs. The proteins identified provide insights into the differentiation of mammary epithelial cells and the regulation of this process.  (+info)

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
The BrdU Chemiluminescent Cell Proliferation Assay Kit is a non-isotopic enzyme immunoassay for the quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is ...
Creative Bioarray provides cell proliferation assay service for our customer. We are capable of performing different cell proliferation assays based on several concepts, which are measuring rate of DNA replication, analysis of metabolic activity, cell surface antigen recognitions, detecting proliferation markers, ATP measurement, measures of membrane integrity and so on.
... Abstract ...The cell proliferation assay is an important tool in the assessment of... Introduction ...Cell proliferation assays are employed frequently in immunological ca...,Cell,Proliferation,Assays,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
BioAssay record AID 1077586 submitted by ChEMBL: Antiproliferative activity against human PLC/PRF/5 cells assessed as inhibition of cell proliferation at 10 to 100 uM by MTT dye uptake assay.
Little knockdown but big effect on proliferation - posted in siRNA, microRNA and RNAi: Hi folks,Hope you are well all !! I did knockdown lately on cell line expressed in GOI and it was tested by qPCR. The results showed that i got moderate suppression in mRNA level in my GOI, following the knockdown i did cell proliferation ( cell viability ) and the results showed that there is an effect on proliferation rate.]My question is how was an effect on proliferation when there was little...
We have shown that 4-1BB in myeloid cells, particularly in APCs, negatively regulates peripheral T cell responses in vivo. Thus, adoptively transferred CD4+ and CD8+ T cells showed the enhanced T cell proliferation in tumor-bearing RAG2−/−4-1BB−/− mice, and DCs and IL-15 were primarily responsible for this enhanced T cell proliferation. However, isolated 4-1BB+/+ and 4-1BB−/− DCs made comparable levels of IL-15, and individual 4-1BB−/− DCs induced T cell responses in vivo less efficiently than 4-1BB+/+ DCs. Therefore, we concluded that 4-1BB deficiency in myeloid cells in vivo induces the enhanced proliferation of peripheral T cells by promoting the accumulation of DCs in secondary lymphoid organs.. Although enhanced T cell responses were first reported when 4-1BB−/− mice were generated more than a decade ago (29), the underlying mechanism was not understood. Other studies have also pointed to a negative regulatory function of 4-1BB in modulating T cell responses. Thus, ...
Creative Proteomics offers sensitive, reliable, and accurate Cell Proliferation Assay via DNA Synthesis to study cell proliferation.
A serum-free or serum-depleted medium for the short- and long-term proliferation and development of cells, particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development effective amounts of: a standard culture medium such as Iscoves modified Dulbeccos medium; serum albumin; transferrin; a source of lipids and fatty acids; cholesterol; a reducing agent; pyruvate; a glucocorticoid (when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth factor, fibroblast growth factor, platelet derived growth factor, and insulin; and extracellular matrix materials, such as collagen, fibronectin, and laminin.
NSCLC is the most prevalent cancer types and has highest mortality rate in China [1]; however, the progression mechanisms of NSCLC have largely remained elusive. Ample evidence indicates a crucial role for miRNAs in human cancer [21], especially the miRNAs participate in the initiation, promotion, and progression of NSCLC. For instance, miR-21 promotes growth and invasion of NSCLC [22]. In addition, miR-494, miR-101, miR-1254, miR-574-5p, miR-143 and miR-181a were demonstrated to be involved in NSCLC [23-25]. In the present study, we certified that miR-361-3p was frequently down-regulated in NSCLC, and first found that the reduced miR-361-3p expression was closely related to advanced stage and lymph node metastasis of NSCLC. Furthermore, we demonstrated that overexpression of miR-361-3p could suppress NSCLC cell proliferation, migration and invasion in vitro and in vivo. The versatile functions of miR-361-3p in tumor cell proliferation, migration and invasion suggest its potential application as ...
Breast cancer is one of the most lethal types of cancer in women worldwide due to the late stage detection and resistance to traditional chemotherapy. The human epidermal growth factor receptor 2 (HER2) is considered as a validated target in breast cancer therapy. Even though a substantial effort has been made to develop HER2 inhibitors, only lapatinib has been approved by the U.S. Food and Drug Administration (FDA). Side effects were observed in a majority of the patients within one year of treatment initiation. Here, we took advantage of bioinformatics tools to identify novel effective HER2 inhibitors. The structure-based virtual screening combined with ADMET (absorption, distribution, metabolism, excretion and toxicity) prediction was explored. In total, 11,247 natural compounds were screened. The top hits were evaluated by an in vitro HER2 kinase inhibition assay. The cell proliferation inhibition effect of identified inhibitors was evaluated in HER2-overexpressing SKBR3 and BT474 cell lines. We
CD44 is a causal factor for tumor invasion, metastasis and acquisition of resistance to apoptosis. CD44 knockdown using inducible short hairpin RNA (shRNA) significantly reduces cell growth and invasion. Short hairpin RNA against CD44 and pGFP-V-RS-vector was used for knockdown of CD44 expression in SW620 colon cancer cells. Cell growth, invasion and migration assay, immunofluorescence for β-catenin expression and western blotting for Wnt signaling molecules were analyzed. Cell cycle analysis and western blot analysis for apoptotic molecules were evaluated. Short hairpin RNA against CD44 reduced the expression of CD44. Cell proliferation, migration and invasion were markedly inhibited and apoptosis was increased in shRNA CD44-transfected cells. Knockdown of CD44 decreased the phosphorylation of PDK1, Akt and GSK3β, and β-catenin levels. Decreased phosphorylated Akt led to an increase in phosphorylated FoxO1 and induced cell cycle arrest in the G0-G1 phase and a decrease in the S phase. The ...
proliferation cp1h2 2 signarrays cell proliferation cp1h2 2 signarrays | order proliferation cp1h2 2 signarrays cell proliferation cp1h2 2 signarrays | How to use: pro
Perifosine and CCI 779 co-operate to induce cell death and decrease proliferation in PTEN-intact and PTEN-deficient PDGF-driven murine glioblastoma.
Plants and animals evolved significantly different strategies of regulation of the cell proliferation and differentiation. Identification and comparison of molecular mechanisms driving those different strategies in plants, animals and microorganisms might contribute to the identification of their common principles. The project is based on collaboration of laboratories involved in the study of the processes of the cell proliferation and differentiation in those different model organisms including human beings. Collaboration of the laboratories with expertise in (i) molecular plant physiology, (ii) molecular mechanisms maintaining the genome stability, (iii) proliferation, differentiation and programmed cell death of cancer cells and (iv) bioinformatics will result into a multidisciplinary, while still functional team that will guarantee successful solution of scheduled tasks. Participation of multiple, recently collaborating laboratories including Core lab will allow effective employment of ...
: Migration and invasion enhancer 1 (MIEN1) is a membrane-anchored protein and exists in various cancerous tissues. However, the roles of MIEN1 in prostate cancer have not yet been clearly addressed. We determined the expression, biological functions, and regulatory mechanisms of MIEN1 in the prostate. The results of immunohistochemical analysis indicated that MIEN1 was expressed specifically in epithelial cells and significantly higher in adenocarcinoma as compared to in normal tissues. MIEN1 enhanced in vitro cell proliferation, invasion, and in vivo tumorigenesis. Meanwhile, MIEN1 attenuated cisplatin-induced apoptosis in PC-3 cells. Overexpression of NF-ĸB-inducing kinase (NIK) enhanced MIEN1 expression, while overexpression of NF-ĸB inhibitor α (IĸBα) blocked MIEN1 expression in PC-3 cells. In prostate carcinoma cells, MIEN1 provoked Akt phosphorylation; moreover, MIEN1 downregulated N-myc downstream regulated 1 (NDRG1) but upregulated interleukin-6 (IL-6) gene expression. MK2206, an
ATCC Cell Proliferation Assay kits are convenient and valuable tools for the quantitative evaluation of a cell populations response to external factors that affect cell viability and growth.
Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22 ...
Gentaur molecular products has all kinds of products like :search , Trevigen \ MTT Cell Proliferation Assay Kit \ 4890-25-K for more molecular products just contact us
Effect of GPC3 on cell proliferation and clonogenic capacity of liver CD90+GPC3+CSCs.(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90+GPC3+
A colorimetric cell proliferation assay using soluble tetrazolium sodium [(CellTiter 96? Aqueous One Remedy) cell proliferation reagent, comprising the (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) and an electron coupling reagent phenazine ethosulfate], was optimized and certified for quantitative dedication of IL-15 dependent CTLL-2 cell proliferation activity. of scripts written in the R Statistical Language and Environment utilizing a […]. ...
Fig. 2. Effect of low-dose PTX (A), BMS-275183 (B), EpoB (C), and 4-HC (D) on in vitro cell proliferation. The antiproliferative effects of the drugs were studied using both short-term (24 h, left) and prolonged continuous exposures (144 h, right) on the HUVEC, HMVEC-d, MDA-MB-435, T0.1, and NHDF cell lines. Columns and bars, mean values ± SE, respectively. aP , 0.05 versus HUVEC controls; bP , 0.05 versus HMVEC-d controls; cP , 0.05 versus MDA-MB-435 controls; dP , 0.05 versus T0.1 controls; eP , 0.05 versus NHDF controls; ∗P , 0.05 versus 144 h HUVEC controls; °P , 0.05 versus 144 h MDA-MB-435 controls; +P , 0.05 versus 24 h HUVEC controls; °P , 0.05 versus 24 h MDA-MB-435 controls.. ...
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we fou …
Introduction The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone. Materials and methods The regulation of gene expression was determined using real-time RTPCR (qRTPCR) and cell proliferation was measured by thymidine incorporation or fluorescent analysis of DNA content in MC3T3E1 osteoblast-like cells. Photomicroscopy was used to identify newly mineralized tissue and fluorescence was used to quantify mineralization. Results Fibroblast growth factor-2 (FGF-2) had the greatest ability to induce proliferation after 24 hours of treatment when compared to transforming growth factor beta (TGFβ, insulin-like growth factor-1 (IGF-1), bone morphogenic protein (BMP-2), platelet derived growth factor (PDGF) or prostaglandin E2 (PGE2). We found that FGF-2 caused the most significant induction of ...
Researchers at Joslin have found that increasing the proliferation and turnover of beta cells before signs of type 1 diabetes could halt the development of the disease.
Cell proliferation assays are performed by four decades in cancer research to test the anti-proliferative activity of natural products and synthetic compounds in in vitro tumor models such as cell lines. However, current parameters used to quantify growth inhibition lead to a misinterpretation of results based on the exponential, and not linear, proliferation of cells in culture. We recently published in Journal of Cellular Physiology the development and experimental validation of a new parameter for the analysis of growth inhibition in proliferation assays, termed relative doubling capacity, that can be used to properly quantify the anti-proliferative activity of tested compounds in cell cultures and compare drug efficacy between distinct cell models. ...
[109 Pages Report] Check for Discount on Global Cell Proliferation Kit Sales Market Report 2017 report by QYResearch Group. In this report, the global Cell Proliferation Kit market is...
Definition of Cell proliferation with photos and pictures, translations, sample usage, and additional links for more information.
Figure 1: Effects of PKR on the proliferation and translation. (a) Effects of PKR on the proliferation of HeLa cells. After being transfected with plasmids PKR, PKR siRNA, or GFP, HeLa cells were plated in multiple wells of a 96-well plate and grown for 24 hr for cell proliferation assays. Cells from the sample preparations were collected for immunoblotting. Proliferation rate of the control sample was normalized to 100%. PKR, WT PKR; si-PKR, PKR siRNA; Ctrl, GFP. Upper panel, averaged data (N=4 ...
The master regulator p53 is a prominent tumor suppressor gene, functioning in the cell as a tetrameric (dimer of dimers) sequence-specific transcription factor, able to bind to two copies of a decameric sequence with the RRRCWWGYYY consensus (where R stands for a purine, W for A/T and Y for a pyrimidine) representing the so called p53-response element (p53-RE) [1]. p53 is known to be inducible in response to a large number of cellular stress signals that, besides genotoxic stress, include carbon and oxygen deficiencies, perturbations of the translation apparatus, excessive proliferation signals, alteration in microtubule dynamics [2, 3]. There are ,100 established p53 targets genes that link p53 to cell cycle arrest, apoptosis, DNA repair and inhibition of angiogenesis [3-6]. More recently, p53 was demonstrated to modulate the expression of genes able to modify glucose as well as lipid metabolism, induction of autophagy, immune responses and cell motility [7-11].. A direct role of p53 on the ...
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2021 Elsevier B.V We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. ...
Cell proliferation analyses are crucial for cell growth and differentiation studies, and are often used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development. Proliferation measurements are typically based on average DNA content or cellular metabolism, or they quantify DNA synthesis.
Molecular mechanism of microRNA-93 in suppressing the cell proliferation of cervical cancer by adjusting the EGFR/AKT signal pathway, Xue Dong, Yan Wang
MicroRNA-331-3p promotes cell proliferation and invasion in breast cancer by targeting SRCIN1, Yonghua Hu, Wenjun Liu, Che Chen, Aihua Hou
Find and order buffers and products like this Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1 on www.antibodies-onli... | Order product ABIN1304384.
Abstract: miRNA-221 was a carcinogenic factor in many cancers, however, it is limited that the correlation between miRNA-221 and progression of cervical cancer. So we here aimed to determine the function of miRNA-221 in cervical cancer proliferation and a
Concurrent in vivo proliferation and CTL activity by gB-specific CD8+ T cells in the PLNs after cutaneous infection with HSV-1. (A) CFSE-labeled lymph node cell
To determine the effect of protein treatments in cell proliferation, MDA-MB-231 breast cancer cells were first injected into the mammary fat pad of nude mice, and when tumors grew to 10 mm in diameter, mice were treated once only with purified Endo (20 mg/kg), CD (40 mg/kg), or EndoCD (60 mg/kg) proteins plus 500 mg/kg 5-FC, a clinically sufficient dose of 5-FU (15 mg/kg; 1× 5-FU), or 10 times the clinically sufficient dose (150 mg/kg; 10× 5-FU). The choice of 20 mg/kg Endo was based on a previous preclinical study (15) and is also within the dose tested in the phase I clinical trial (ref. 18; 15-600 mg/m2 in human is equivalent to 4.8-194.4 mg/kg in mouse; ref. 30). Tumors were harvested from mice 48 hours after treatment and labeled with bromodeoxyuridine (BrdU) antibody for in vivo BrdU incorporation analysis. The results show that EndoCD/5-FC most significantly reduced cancer cell proliferation (Fig. 2B) compared with all other treatment groups.. The potent inhibitory activity of ...
Trouvez tous les livres de Lam, Eric W-F - Phosphoinositide 3-Kinase Signalling Pathway: The Key to Cell Proliferation and Death. Sur,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9781860946264
This inhibition concerns cell proliferation and the expression of IL-8, u-PA, and MMP - 9, which can AZD0530 proposes inhibit invasion of cancer cells in the
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Gen5 allows measuring cell proliferation as both an end point and kinetic imaging assay and can be run with temperature and gas control.
A method for treating a disorder characterized by excessive cell proliferation in a patient by administering to the patient a therapeutically effective amount of sclareolide.
cell proliferation - Read articles from Issue 2015(12). Read article PDFs using your inistitutions subscriptions with no additional login.
cell proliferation - Read articles from Issue 2009(04). Read article PDFs using your inistitutions subscriptions with no additional login.
Cells are structural and functional unit of our body that control and maintain the function of all unicellular and multicellular organisms
Full Text - Inducing cardiomyocyte proliferation is a hopeful approach for cardiac regeneration following myocardial infarction. Previous studies have shown that p21 inhibits the cardiomyocyte proliferation and cardiac regeneration. Deacetylation of p21 by Sirt1 deacetylase may reduce p21 abundance and remove p21-induced cell cycle arrest. However, whether p21 deacetylation and Sirt1 deacetylate control cardiomyocyte proliferation is unclear. Here, we show that acetylation of p21 induces cardiomyocyte proliferation arrest, whereas blocking the acetylation of p21 increases cardiomyocyte proliferation. P21 can be acetylated by Sirt1, and Sirt1 activate p21 ubiquitination through deacetylation. Additionally, overexpression of Sirt1 induces EdU-, pH3-, and Aurora B-positive cardiomyocytes in neonatal and adult mice. In contrast, depletion of Sirt1 reduces cardiomyocyte proliferation in vitro and in vivo. Moreover, Sirt1 protects cardiac function, reduces cardiac remodeling, inhibits
The functional integrity of the intestinal epithelial barrier relies on tight coordination of cell proliferation and migration, with failure to regulate these processes resulting in disease. It is not known whether cell proliferation is sufficient to drive epithelial cell migration during homoeostatic turnover of the epithelium. Nor is it known precisely how villus cell migration is affected when proliferation is perturbed. Some reports suggest that proliferation and migration may not be related while other studies support a direct relationship. We used established cell-tracking methods based on thymine analog cell labeling and developed tailored mathematical models to quantify cell proliferation and migration under normal conditions and when proliferation is reduced and when it is temporarily halted. We found that epithelial cell migration velocities along the villi are coupled to cell proliferation rates within the crypts in all conditions. Furthermore, halting and resuming proliferation ...
We have previously demonstrated, that 15 days after female rats pace the sexual interaction, there is an increase in the number of new cells that reach the granular cell layer (GrL) of the accessory olfactory bulb (AOB). The aim of the present study was to evaluate, if the first sexual experience in the female rat increases cell proliferation in the subventricular zone (SVZ) and the rostral migratory stream (RMS). We also tested if this behavior promotes the survival of the new cells that integrate into the main olfactory bulb (MOB) and AOB 45 days after the behavioral test. Sexually, naive female rats were injected with the DNA synthesis marker 5′-bromo-2′-deoxyuridine (BrdU) on the day of the behavioral test. They were randomly divided into the following groups: Female rats placed alone in the mating cage (1); Females exposed to amyl acetate odor [banana scent, (2)]; Females that could see, hear, and smell the male but physical contact was not possible [exposed to male, (3)]; Female rats that
Markers of crypt cell proliferation are frequently employed in studies of the impact of genetic and exogenous factors on human colonic physiology. Human studies often rely on the assessment of tissue acquired at endoscopy. Modulation of cell proliferation by bowel preparation with oral laxatives may confound the findings of such studies, but there is little data on the impact of commonly used bowel preparations on markers of cell proliferation. Crypt length, crypt cellularity and crypt cell proliferation were assessed in biopsies acquired after preparation with either Klean-Prep or Picolax. Crypt cell proliferation was assessed by whole-mount mitotic figure count, and by two different immunohistochemical (IHC) labelling methods (Ki-67 and pHH3). Subsequent biopsies were obtained from the same patients without bowel preparation and similarly assessed. Parameters were compared between groups using analysis of variance and paired t-tests. There were significant differences in labelling indices (LI) between
Mesothelin (MSLN) overexpression in pancreatic cancer (PC) leads to enhanced cell survival/proliferation and tumor progression. After screening for a number of growth factors/cytokines, we found that the MSLN expression correlated closely with interleukin (IL)-6 in human PC specimens and cell lines. Stably overexpressing MSLN in different PC cell lines (MIA-MSLN and Panc1-MSLN) led to higher IL-6 production. Silencing MSLN by small interfering RNA (siRNA) significantly reduced IL-6 levels. Blocking the observed constitutive activation of nuclear factor-kappaB (NF-κB) with IKK inhibitor wedelolactone in MIA-MSLN cells also reduced IL-6. Silencing IL-6 by siRNA reduced cell proliferation, cell cycle progression and induced apoptosis with significant decrease of c-myc/bcl-2. Interestingly, recombinant IL-6-induced proliferation of MIA-MSLN cells but not MIA-V cells. Although messenger RNA/protein levels of IL-6R did not vary, soluble IL-6R (sIL-6R) was significantly elevated in MIA-MSLN and was ...
Tumor cell metastasis and proliferation are crucial for tumor development and result in loss of life of tumor individuals. TLR4 signaling pathway. It offers fresh insights for the systems of tumor advancement and metastasis, and suggests targeting TLR4 and OPN as an intervention in the ovarian cancer treatment. proliferation activity of tumor cells. Without LPS stimulation, the proliferation activity of tumor cells increased during 12 h. The cell proliferation significantly changed by LPS stimulation, and the maximum absorbance value at 429 nm was present after 6 h, with a proliferation rate of approximately 137.1% compared to cells without stimulation (Figure 2AC2B). To investigate the effect of TLR4 signal block on cell proliferation, the TLR4 inhibitor TAK-242 was used. The LPS-stimulated increase in the proliferation of tumor cells was significantly reduced with TAK-242 pretreatment, whereas no significant change was observed in cells treated with TAK-242 alone (Figure ?(Figure2C).2C). These ...
PC cell-derived growth factor (PCDGF), also called epithelin/granulin precursor (GEP), is an 88-kDa secreted glycoprotein with the ability to stimulate cell proliferation in an autocrine fashion. In addition, some studies indicated that PCDGF participated in invasion, metastasis and survival of cancer cells by regulating cell migration, adhesion and proliferation. Yet the effects of PCDGF on proliferation and invasion of ovarian cancer cells in vitro and the mechanisms by which PCDGF mediates biological behaviors of ovarian cancer have rarely been reported. In the present study we investigated whether and how PCDGF/GEP mediated cell proliferation and invasion in ovarian cancer. PCDGF/GEP expression level in three human ovarian cancer cell lines of different invasion potential were detected by RT-PCR and western blot. Effects of inhibition of PCDGF expression on cell proliferation and invasion capability were determined by MTT assay and Boyden chamber assay. Expression levels of cyclin D1 and CDK4 and
Regulation of mRNAs is one way to control protein levels and thereby important cellular processes such as growth, invasion and apoptosis. G3BPs constitute a family of mRNA-binding proteins, shown to be overexpressed in several cancer types, including breast, colon and pancreas cancer. G3BP has been reported to both stabilize and induce degradation of specific mRNAs. Here, we show that G3BP1, but not G3BP2, supports proliferation of several breast cancer cell lines. Global gene expression analyses of G3BP1- and G3BP2-depleted cells indicate that primarily G3BP1, and much less G3BP2, influences mRNA expression levels. Peripheral myelin protein 22 (PMP22) was one gene that was significantly influenced by G3BP1 depletion which led to a 2-3 fold increased expression. Depletion of PMP22 resulted in increased proliferation and the G3BP1-mediated effect on proliferation was not seen upon PMP22-depletion. This indicates a novel role for G3BP1 in the regulation of cell proliferation in breast cancer cells,
KDM5c is a histone demethylase that specifically demethylates trimethylated and dimethylated H3 Lys-4 to play a central role in transcriptional repression. C-Jun is a proto-oncogene and promotes cell proliferation when ectopically accumulated, but can be ubiquitinated by SCF (FBXW7), leading to its degradation. FBXW7 is an E3 ubiquitin ligase of c-Jun, and exhibits carcinostasis in colon cancer. Here, we report that overexpression of KDM5c in human colon cancer cells results in attenuated FBXW7 transcription and accumulated c-Jun protein, leading to increased proliferation of colon cancer cells. We show that overexpression of KDM5c can result in increased c-Jun protein levels and decreased ubiquitin levels, with no significant change in mRNA levels of c-Jun. KDM5c overexpression blocks the ubiquitin-proteasome proteolytic pathway of c-Jun by down-regulating the expression of FBXW7. KDM5c down-regulation of FBXW7 occurs by demethylation of H3K4me3 at TSS and downstream of the FBXW7 gene. And interaction
Various assays, using different strategies, are available for assessing cultured cell proliferation. These include measurement of metabolic activity (tetrazolium salts and alamarBlue), DNA quantification using fluorophores (Hoechst 33258 and PicoGreen), uptake of radioactively-labeled DNA precursors such as [3H]thymidine, and physical counting (hemocytometer). These assays are well established in characterizing cell proliferation in two-dimensional (2D), monolayer cultures of low cell densities. However, increasing interest in 3D cultures has prompted the need to evaluate the effectiveness of using these assays in high cell density or 3D cultures. We show here that typical cell proliferation assays do not necessarily correlate linearly with increasing cell densities or between 2D and 3D cultures, and are either not suitable or only rough approximations in quantifying actual cell numbers in a culture. Prudent choice of techniques and careful interpretation of data are therefore recommended when ...
p,Here, we ask how neural stem cells (NSCs) transition in the developing neocortex from a rapidly to a slowly proliferating state, a process required to maintain lifelong stem cell pools. We identify LRIG1, known to regulate receptor tyrosine kinase signaling in other cell types, as a negative regulator of cortical NSC proliferation. LRIG1 is expressed in murine cortical NSCs as they start to proliferate more slowly during embryogenesis and then peaks postnatally when they transition to give rise to a portion of adult NSCs. Constitutive or acute loss of Lrig1 in NSCs over this developmental time frame causes stem cell expansion due to increased proliferation. LRIG1 controls NSC proliferation by associating with and negatively regulating the epidermal growth factor receptor (EGFR). These data support a model in which LRIG1 dampens the stem cell response to EGFR ligands within the cortical environment to slow their proliferation as they transition to postnatal adult NSCs.,/p,. ...
TY - JOUR. T1 - α-1 Adrenergic receptors stimulation induces the proliferation of neural progenitor cells in vitro. AU - Hiramoto, Takeshi. AU - Ihara, Yoshiaki. AU - Watanabe, Yasuhiro. PY - 2006/11/6. Y1 - 2006/11/6. N2 - The proliferation of neural progenitor cells (NPCs) is regulated by classical neurotransmitters such as dopamine, serotonin and acetylcholine, via its own receptors. Previous studies have reported that the depletion of l-norepinephrine decreases the proliferation of NPCs in the adult rat hippocampus and it has been suggested that l-norepinephrine regulates the proliferation of NPCs. However, it remains unknown whether or not adrenergic receptors are involved in the increased proliferation of NPCs. In the present study, an MTT cell proliferation assay was carried out in order to investigate the roles played by adrenergic receptors in the proliferation of NPCs. We demonstrated that l-epinephrine enhanced the proliferation of embryonic NPCs in vitro. In addition, the α-1 ...
TY - JOUR. T1 - Conflicting evidence for the role of JNK as a target in breast cancer cell proliferation. T2 - comparisons between pharmacological inhibition and selective shRNA knockdown approaches. AU - Wood, Rachel A.. AU - Barbour, Mark J.. AU - Gould, Gwyn W.. AU - Cunningham, Margaret R.. AU - Plevin, Robin J.. N1 - © 2017 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.. PY - 2018/2/28. Y1 - 2018/2/28. N2 - As a target, the JNK pathway has been implicated in roles including cell death, proliferation, and inflammation in variety of contexts which span cardiovascular disease, neurodegenerative pathologies, and cancer. JNK1 and JNK2 have recently been demonstrated to function independently, highlighting a new parameter in the study of the JNK pathway. In order for JNK1 and JNK2-specific roles to be defined, better tools need to be employed. Previous ...
OBJECTIVE: This study explored the effect of miR-26a-5p on cell proliferation, migration, and invasion in gastric cancer by targeting COL10A1. MATERIALS
ADSCs are a promising candidate for clinical applications. They are easily derived from adipose tissue, with strong proliferation potential. Compared with other sources of MSCs such as bone marrow, umbilical cord and umbilical cord blood, ADSCs have a shorter doubling time. Therefore, numerous studies on ADSCs, especially on ADSC in vitro proliferation, have been performed to date. To date, hypoxia has been applied on stem cell cultures including ADSC culture. This study aimed to evaluate the effects of hypoxia on ADSC proliferation.. First, ADSCs were successfully isolated and confirmed by usual methods and assays. Similarly to previous studies, the obtained ADSCs fully exhibited several MSCs characteristics, including adherence to the surface of plastic flasks with a fibroblast-like shape; they also successfully differentiated into three kinds of mesoderm cells including adipocytes, osteoblasts and chondroblasts. Flow cytometry analysis with surface markers revealed that they expressed ...
F the soft agar colony formation in comparison with vector control cells exposed to arsenite for eight weeks. A single explanation of these information is the
Measurement of cell proliferation is necessary for testing the effects of pharmacological agents or growth factors, assessing cytotoxicity or investigating circumstances of cell activation. In a cell proliferation assay, the increase in number of cells or change in the proportion of cells that is dividing is assessed. Trevigen® offers a Calcein-AM based kit to determine cell viability as well as the tetrazolium salts MTT and XTT metabolic cell proliferation assay kits.. ...
Cell proliferation is a process of increasing cell number, including cell development and cell division [46]. This intern responsible to increase in cell mass and ultimately the organism development. In certain cells, proliferation is restricted while it remains continue in some of the cells throughout lifetime. In certain situation cell proliferation becomes abnormal which further augment the tumor cell formation [40]. In the body, cell proliferation process is highly regulated by cell division and apoptosis [2]. Apoptosis is programmed cell death mechanism that destroys unwanted cells and also involved in defense mechanism of the body. It also plays a role in morphological and cellular mechanisms of cell, caspases functioning, translocation of phosphatidyl serine and DNA fragmentation [43, 47]. This complex process comprises intrinsic pathway and extrinsic pathway which are also called as mitochondrial pathway and cytoplasmic pathway or death receptor pathway, respectively (Fig. 4) [5]. In ...
This paper represents a study on the effect of electrical pulses on adult stem cells, especially on proliferation control and also as a method of deliverin
TY - JOUR. T1 - Tumor growth dynamics with nutrient limitation and cell proliferation time delay. AU - Alsheri, Ahuod. AU - Alzahrani, Ebraheem O.. AU - Asiri, Asim. AU - El-Dessoky, Mohamed M.. AU - Kuang, Yang. PY - 2017/12/1. Y1 - 2017/12/1. N2 - It is known that avascular spherical solid tumors grow monotonically, often tends to a limiting final size. This is repeatedly confirmed by various mathematical models consisting of mostly ordinary differential equations. However, cell growth is limited by nutrient and its proliferation incurs a time delay. In this paper, we formulate a nutrient limited compartmental model of avascular spherical solid tumor growth with cell proliferation time delay and study its limiting dynamics. The nutrient is assumed to enter the tumor proportional to its surface area. This model is a modification of a recent model which is built on a two-compartment model of cancer cell growth with transitions between proliferating and quiescent cells. Due to the limitation of ...
The Wnt signal pathway is composed of β-catenin and transcriptional factor TCF-4 (2 , 3 , 19, 20, 21) . These factors activate the target genes that preserve the consensus motif (A/TA/TCAAAG) for TCF-4 binding in the promoter region (6) . Recent studies have shown that cyclin D1, c-myc, MMP7, and c-jun could be target genes for the Wnt signal pathway (7, 8, 9, 10) . Therefore, Wnt signal may induce cell proliferation through activation of these target genes (22, 23, 24, 25) . To our knowledge, there is no report on RCCs regarding the significance of the Wnt signal factors in cell proliferation and/or apoptosis through regulation of downstream target genes. In this study, we evaluated the significance of the Wnt signal pathway in RCC through the analysis of cyclin D1, c-myc, MMP7, and c-jun in relation to β-catenin and TCF-4 alterations.. TCF-4 expression has been intensively investigated in the epithelium of the gastrointestinal tract (26 , 27) , and the presence of splicing isoforms in TCF-4 ...
Transforming growth factor-β (TGF-β) plays an important role in regulating hematopoiesis, inhibiting proliferation while stimulating differentiation when appropriate. We previously demonstrated that the type III TGF-β receptor (TβRIII, or betaglycan) serves as a novel suppressor of cancer progression in epithelial tumors; however, its role in hematologic malignancies is unknown. Here we demonstrate that TβRIII protein expression is decreased or lost in the majority of human multiple myeloma specimens. Functionally, restoring TβRIII expression in myeloma cells significantly inhibited cell growth, proliferation, and motility, largely independent of its ligand presentation role. In a reciprocal fashion, shRNA-mediated silencing of endogenous TβRIII expression enhanced cell growth, proliferation, and motility. Although apoptosis was not affected, TβRIII inhibited proliferation through induction of the cyclin-dependent kinase inhibitors p21 and p27. TβRIII further regulated myeloma cell ...
Genome-wide mutant strain collections have increased demand for high throughput cellular phenotyping (HTCP). For example, investigators use HTCP to investigate interactions between gene deletion mutations and additional chemical or genetic perturbations by assessing differences in cell proliferation among the collection of 5000 S. cerevisiae gene deletion strains. Such studies have thus far been predominantly qualitative, using agar cell arrays to subjectively score growth differences. Quantitative systems level analysis of gene interactions would be enabled by more precise HTCP methods, such as kinetic analysis of cell proliferation in liquid culture by optical density. However, requirements for processing liquid cultures make them relatively cumbersome and low throughput compared to agar. To improve HTCP performance and advance capabilities for quantifying interactions, YeastXtract software was developed for automated analysis of cell array images. YeastXtract software was developed for kinetic growth
Genome-wide mutant strain collections have increased demand for high throughput cellular phenotyping (HTCP). For example, investigators use HTCP to investigate interactions between gene deletion mutations and additional chemical or genetic perturbations by assessing differences in cell proliferation among the collection of 5000 S. cerevisiae gene deletion strains. Such studies have thus far been predominantly qualitative, using agar cell arrays to subjectively score growth differences. Quantitative systems level analysis of gene interactions would be enabled by more precise HTCP methods, such as kinetic analysis of cell proliferation in liquid culture by optical density. However, requirements for processing liquid cultures make them relatively cumbersome and low throughput compared to agar. To improve HTCP performance and advance capabilities for quantifying interactions, YeastXtract software was developed for automated analysis of cell array images. YeastXtract software was developed for kinetic growth
Lack of IGF2 in mice results in diminished embryonic growth due to diminished cell proliferation. Here we show that mouse embryonic fibroblasts lacking the RNA-binding protein IMP1 (IGF2 mRNA-binding protein 1) have defective splicing and translation of IGF2 mRNAs, markedly reduced IGF2 polypeptide production, and diminished proliferation. The proliferation of the IMP1-null fibroblasts can be restored to wild-type levels by IGF2 in vitro or by re-expression of IMP1, which corrects the defects in IGF2 RNA splicing and translation. The ability of IMP1 to correct these defects is dependent on IMP1 phosphorylation at Ser181, which is catalyzed cotranslationally by mTOR complex 2 (mTORC2). Phosphorylation strongly enhances IMP1 binding to the IGF2-leader 3 5 untranslated region, which is absolutely required to enable IGF2-leader 3 mRNA translational initiation by internal ribosomal entry. These findings uncover a new mechanism by which mTOR regulates organismal growth by promoting IGF2 production in ...
Visit to view our Cellular Assay Kits materials including Cell Proliferation Assays & more. CST - Customer satisfaction is our highest priority.
Visit to view our Cellular Assay Kits materials including Cell Proliferation Assays & more. CST - Customer satisfaction is our highest priority.
Visit to view our Cellular Assay Kits materials including Cell Proliferation Assays & more. CST - Customer satisfaction is our highest priority.
Fibulins not only function as molecular bridges within the cellular microenvironment but also influence cell behavior. Thus, fibulins may contribute to create a permissive microenvironment for tumor growth but can also stimulate different mechanisms that may impede tumor progression. This is the case with Fibulin-5, which has been shown to display both tumor-promoting and tumor-protective functions by mechanisms that are not totally defined. We show new evidence on the tumor-protective functions displayed by Fibulin-5 in MCF-7, T47D and MDA-MB-231 breast cancer cells including the inhibition of invasion and proliferation capacity and hampering the ability to form mammospheres. Reduction in the level of phosphorylation of Ser residues involved in the nuclear translocation of β-catenin may underlie these antitumor effects. We also found that Fibulin-5 reduces the level of expression of Ki-67, a nuclear protein associated with cell proliferation. Moreover, reduction in Fibulin-5 expression ...
Non-small cell lung cancer (NSCLC) is a type of malignant tumor which threatens human health and life. Recently, some researches on long non-coding RNAs (lncRNAs) in NSCLC has elucidated critical regulatory roles in cell proliferation, migration, and invasion, the relative clinical significance and mechanisms of action are still unclear. This study focuses on the important role of a novel lncRNA LINC00665 in the development of NSCLC. Long intergenic non-protein coding RNA 665 gene (LINC00665) was found through microarray analysis and was measured by real-time quantitative PCR (RT-qPCR). The interactions between LINC00665 and miR-138-5p as well as the interactions between miR-138-5p and E2F3 (E2F transcription factor 3) were explored by bioinformatics analysis and dual-luciferase assays. CCK-8, transwell and mouse xenograft assays were performed to investigate the effects of LINC00665 and miR-138-5p on NSCLC proliferation and invasion. As a result, LINC00665 expression was upregulated in NSCLC ...
The present study, to the best of the authors knowledge, demonstrated for the first time, that miR-614 was upregulated in OC clinical tissues and cells. Increased expression of miR-614 led to the promotion of cell proliferation and colony-forming abilities, and conversely, decreased the apoptotic rate of OC cells. Additionally, PPP2R2A may act as a novel target of miR-614. The present study indicated that miR-614 may act as a novel tumor promoter in OC by targeting PPP2R2A.. Accumulating evidence suggests that miRNAs exhibit an essential role in human cancer pathological proceedings via controlling different target genes, including those involved in cell proliferation, migration, invasion, cycle and apoptosis (15-18). Dysregulation of miRNA frequently occurs in novel types of cancers, including ovarian cancer. miR-21-3p inhibits cell proliferation and invasion of ovarian cancer by targeting RNA binding protein with multiple splicing, RCC1 and BTB domain containing protein 1 and Zinc finger ...
miRNAs are emerging as critical regulators in carcinogenesis and tumor progression. Recently, microRNA-122 (miR-122) has been proved to play an important role in hepatocellular carcinoma, but its functions in the context of breast cancer (BC) remain unknown. In this study, we report that miR-122 is commonly downregulated in BC specimens and BC cell lines with important functional consequences. Overexpression of miR-122 not only dramatically suppressed cell proliferation, colony formation by inducing G1-phase cell-cycle arrest in vitro, but also reduced tumorigenicity in vivo. We then screened and identified a novel miR-122 target, insulin-like growth factor 1 receptor (IGF1R), and it was further confirmed by luciferase assay. Overexpression of miR-122 would specifically and markedly reduce its expression. Similar to the restoring miR-122 expression, IGF1R downregulation suppressed cell growth and cell-cycle progression, whereas IGF1R overexpression rescued the suppressive effect of miR-122. To identify
Both cell proliferation and cell size control are fundamental biological processes that must be carefully orchestrated, and dysregulation of either can lead to diseases such as cancer. In contrast to our understanding of the mechanisms that control cell proliferation, less is known about the mechanisms that control cell size and, particularly, the mechanisms by which cell proliferation and cell size are coordinately regulated. Recently, we identified a novel protein named FIP200, which plays an important role in the regulation of cell cycle progression (Abbi et al., 2002). In this study, we showed that FIP200 can also regulate cell size through interaction with the TSC1-TSC2 complex and activation of S6K. These results identify FIP200 as a regulator that plays roles in both cell proliferation and cell size control.. Most other proteins known to play roles in both cell proliferation and cell size usually regulate these two cellular processes in a similar manner. For example, PTEN can inhibit cell ...
39; re advancing for cannot contend formed, it may be reasonably malformed or then designed. If the incentive is, please want us update. 2018 Springer Nature Switzerland AG. The Incomplete includes due processed. Sign the anything of over 341 billion g subjects on the loan. Prelinger Archives F currently! The example you compile arrested had an likelihood: range cannot take called. We wish teachings to result ia with our browser great and stepwise, to better review the link of our documents, and to exercise family. For further download Trends in Stem Cell Proliferation and Cancer Research, including about email languages, gain undo our Cookie Policy. 2018 The download Trends in Stem Cell terminology; Expression Company, LLC. CloseBasicsOverviewBasic FactsQuality of LifePeopleLanguageLegal SystemAboriginalsEducationEconomyMoneyMilitaryForeign PolicyHistoryOverviewEarly History19th Century20th Century21st CenturyPrime MinistersJohn A. BasicsOverviewBasic FactsQuality of LifePeopleLanguageLegal ...
Ng F, Ye J, et al. PERK promotes cancer cell proliferation and tumor development by limiting oxidative DNA harm. Oncogene 29: 38813895. 42. Min L, Ji Y, Bakiri
Antibodies for proteins involved in positive regulation of B cell proliferation pathways, according to their Panther/Gene Ontology Classification
Supervisor: Golnar Kolahgar. The intestinal epithelium constantly regenerates from stem cells, which adjust their behaviour to the changing physiological conditions the gut is exposed to. For example, stem cell proliferation rates can transiently increase to speed up regeneration after tissue loss or in response to the diet, before reverting to steady-state levels once correct tissue size is reached. This plasticity is essential for intestinal function, as lack of regeneration causes tissue atrophy whereas unrestricted stem cell proliferation promotes cancer. We use the genetically tractable Drosophila gut to identify the secreted and physical factors regulating gut plasticity. In particular, we use targeted genetic screens and functional analyses, to identify novel extracellular signalling molecules regulating cell proliferation in contexts that trigger reversible changes in gut size. As the regulation of intestinal proliferation is largely conserved between Drosophila and mammals this work has ...
The Hippo-Yap signaling pathway regulates a number of developmental and adult cellular processes, including cell fate determination, tissue growth, and tumorigenesis. Members of the scaffold protein angiomotin (Amot) family interact with several Hippo pathway components, including Yap (Yes-associated protein), and either stimulate or inhibit Yap activity. We used a combination of genetic, biochemical, and transcriptional approaches to assess the functional consequences of the Amot-Yap interaction in mice and in human cells. Mice with a liver-specific Amot knockout exhibited reduced hepatic oval cell proliferation and tumorigenesis in response to toxin-induced injury or when crossed with mice lacking the tumor suppressor Nf2. Biochemical examination of the Amot-Yap interaction revealed that the p130 splicing isoform of Amot (Amot-p130) and Yap interacted in both the cytoplasm and nucleus, which involved binding of PPxY and LPxY motifs in Amot-p130 to WW domains of Yap. In the cytoplasm, ...
ECM components are the key players of 3D culture methods: ECM are tissue specific and are composed of a wide array of molecules including collagens, elastin, glycosaminoglycans, growth factors, proteoglycans; each of which plays a crucial role. For example, collagen and fibronectin are known to influence cell migration whereas integrin dependent signaling cascade influences cell proliferation and polarity.. In an experiment, 2D culture of human breast epithelial cells resulted in tumor cell like growth whereas the same cell when cultured 3 dimensionally, the cell growth was back to normal. This undoubtedly highlights the importance of ECM in regulating complex cellular responses. Over the past decade different methods for culturing cells three dimensionally have been developed. They can be broadly classified as -. 1.Scaffold based techniques Here cells are cultured in presence of cell specific ECM like matrices. Today a plethora of ECM like scaffolds have been designed and incorporated in cell ...
Reduction of Prep1 Levels Affects Differentiation of Normal and Malignant B Cells and Accelerates Myc Driven Lymphomagenesis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Background: Cisplatin, a DNA damaging agent, induces apoptosis through increasing DNA fragmentation.However, identification of intrinsic resistance molecules against Cisplatin is vital to estimate the success of therapy.Bag-1 (Bcl-2-associated anthanogene) is one anti-apoptotic protein involved in drug resistance impacting ontherapeutic efficiency. Elevated levels of this protein are related with increase cell proliferation rates, motilityand also cancer development. For this reason, we aimed to understand the role of Bag-1 expression in Cisplatininducedapoptosis in HeLa cervix cancer cells. Cisplatin decreased cell viability in time- and dose-dependentmanner in wt and Bag-1L+HeLa cells. Although, 10μM Cisplatin treatment induced cell death within 24h byactivating caspases in wt cells, Bag-1L stable transfection protected cells against Cisplatin treatment. To assess thepotential protective role of Bag-1, we first checked the expression profile of interacting anti-apoptotic partners ofBag-1. We found
RESULTS: 775 genes were differentially expressed and clustered in terms of their growth factor responsiveness. As well as identifying uncharacterized genes as novel targets of ErbB2-dependent signalling, ErbB2 overexpression augmented the induction of multiple genes involved in proliferation (e.g. MYC, MAP2K1, MAP2K3), autocrine growth factor signalling (VEGF, PDGF) and adhesion/cytoskeletal regulation (ZYX, THBS1, VCL, CNN3, ITGA2, ITGA3, NEDD9, TAGLN), linking them to the hyper-poliferative and altered adhesive phenotype of the ErbB2-overexpressing cells. We also report ErbB2-dependent down-regulation of multiple interferon-stimulated genes that may permit ErbB2-overexpressing cells to resist the anti-proliferative action of interferons. Finally, IGFBP3 was unique in its pattern of regulation and we further investigated a possible role for IGFBP3 down-regulation in ErbB2-dependent transformation through suppressed IGF1 signalling. We show that IGF1-dependent signalling and proliferation were ...
The application discloses novel 2-alkoxyestradiol analogs which exhibit anti-proliferative properties, and methods of making and using such compounds to inhibit undesired cell proliferation and tumor growth. Additionally, methods are disclosed of treating diseases associated with undesired angiogenesis and undesired proliferation, and methods of treating infectious disease wherein the infectious agent is particularly susceptible to inhibition by agents that disrupt microtubule organization and function.
... is the process by which a cell grows and divides to produce two daughter cells. Cell proliferation leads to ... Cell proliferation requires both cell growth and cell division to occur at the same time, such that the average size of cells ... Cell proliferation occurs by combining cell growth with regular "G1-S-M-G2" cell cycles to produce many diploid cell progeny. ... The total number of cells in a population is determined by the rate of cell proliferation minus the rate of cell death. Cell ...
Cell Proliferation is a monthly open-access online-only scientific journal covering cell biology. It was established in 1968 as ... "Cell Biology". Zhou, Qi (June 2018). "Editorial". Cell Proliferation. 51 (3): e12448. doi:10.1111/cpr.12448. PMC 6528904. PMID ... "Journals Ranked by Impact: Cell Biology". 2021 Journal Citation Reports. Web of Science (Science ed.). Clarivate Analytics. ... Cell and Tissue Kinetics, obtaining its current title in 1991. It is published by John Wiley & Sons and the editor-in-chief is ...
Detection of apoptosis and cell proliferation". Cell Proliferation. 28 (11): 571-579. doi:10.1111/j.1365-2184.1995.tb00045.x. ... Analogy to apoptosis of somatic cells". Exp Cell Res. 207 (1): 202-205. doi:10.1006/excr.1993.1182. PMID 8391465. Lozano GM, ... "In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations". J Histochem Cytochem. 44 ... It may also label cells having DNA damaged by other means than in the course of apoptosis. The fluorochrome-based TUNEL assay ...
It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the ... in HeLa cells. Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the ... cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In ... "Cell cycle dependent expression and stability of the nuclear protein detected by Ki-67 antibody in HL-60 cells". Cell ...
Cell Proliferation. 36 (3): 131-49. doi:10.1046/j.1365-2184.2003.00266.x. PMC 6496723. PMID 12814430. Bertoli C, Skotheim JM, ... After the cell has split into its two daughter cells, the cell enters G1. DNA repair processes and cell cycle checkpoints have ... During this process, known as the cell cycle, a cell duplicates its contents and then divides in two. The purpose of the cell ... and the cell wall forms a septum which divides the cell into two. As the eukaryotic cell cycle is a complex process, eukaryotes ...
"Mathematical model for the cancer stem cell hypothesis". Cell Proliferation. 39 (1): 3-14. doi:10.1111/j.1365-2184.2006.00369.x ... how biological inks can be formulated to print mammalian cells into three-dimensional cellular structures with ...
Since they account for individual behavior at the cell level such as cell proliferation, cell migration or apoptosis, cell- ... update the position of each vertex according to tensions in the cell membrane resulting from cell-cell adhesion forces and cell ... in order to account for cell-cell communication. As such, cell-based models have been used to study processes ranging from ... Cell-based models are mathematical models that represent biological cells as a discrete entities. Within the field of ...
Cell Proliferation. 24 (2): 203-214. doi:10.1111/j.1365-2184.1991.tb01150.x. PMID 2009322. S2CID 37720004. Valko M, Morris H, ... There are about 24,000 oxidative DNA adducts per cell in young rats and 66,000 adducts per cell in old rats. Likewise, any ... Reactive oxygen species can disrupt the function of immune cells, promoting immune evasion of leukemic cells. On the other hand ... In rat urine, about 74,000 oxidative DNA adducts per cell are excreted daily. There is also a steady state level of oxidative ...
Molinari M (October 2000). "Cell cycle checkpoints and their inactivation in human cancer". Cell Proliferation. 33 (5): 261-74 ... Cell division is the process by which a parent cell divides into two daughter cells. Cell division usually occurs as part of a ... Cell fusion gametic fusion Cell growth Cyclin-dependent kinase Labile cells, cells that constantly divide Martin EA, Hine R ( ... Also, the pattern of cell division that transforms eukaryotic stem cells into gametes (sperm cells in males or egg cells in ...
Clarke RB (December 2005). "Isolation and characterization of human mammary stem cells". Cell Proliferation. 38 (6): 375-86. ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ...
"Production of stem cells with embryonic characteristics from human umbilical cord blood". Cell Proliferation. 38 (4): 245-255. ... Hematopoietic stem cells in the bone marrow can give rise to hematopoietic lineage cells, and mesenchymal stem cells, which can ... which are also known as marrow stromal cells. These are multipotent stem cells that can differentiate into a variety of cell ... Bone marrow stem cells can be induced to become neural cells to treat neurological illnesses, and can also potentially be used ...
Cell Proliferation. 39 (2): 147-155. doi:10.1111/j.1365-2184.2006.00377.x. PMC 6496865. PMID 16542349. S2CID 16515437. Tulett, ... "Bayer acquires BlueRock Therapeutics to build leading position in cell therapy". BioSpace. 8 August 2019. Retrieved 27 November ... renal cell carcinoma), and certain types of thyroid cancer. Trasylol (Aprotinin) Trasylol is a trypsin inhibitor used to ...
The Notch pathway plays a role in controlling stem cell proliferation for several cell types including hematopoietic, neural ... "Critical appraisal of the side population assay in stem cell and cancer stem cell research". Cell Stem Cell. 8 (2): 136-47. doi ... They examined cancer stem cell plasticity in which cancer stem cells can transition between non-cancer stem cells (Non-CSC) and ... De-differentiation of mutated cells may create stem cell-like characteristics, suggesting that any cell might become a cancer ...
... cells. DFAT cells re-establish active proliferation ability and express multipotent capacities. Compared with adult stem cells ... May 2013). "TAP-deficient human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen- ... June 2014). "Human somatic cell nuclear transfer using adult cells". Cell Stem Cell. 14 (6): 777-80. doi:10.1016/j.stem.2014.03 ... June 2013). "Human embryonic stem cells derived by somatic cell nuclear transfer". Cell. 153 (6): 1228-38. doi:10.1016/j.cell. ...
As a consequence, unregulated cell proliferation occurs. This cell proliferation caused by nicotine could be blocked by using ... small cell lung cancer proliferation". Cell Proliferation. 41 (6): 936-959. doi:10.1111/j.1365-2184.2008.00566.x. PMC 9531952. ... "Role of alpha7-nicotinic acetylcholine receptor in human non-small cell lung cancer proliferation". Cell Proliferation. 41 (6 ...
Bou-Gharios G, Ponticos M, Rajkumar V, Abraham D (2004). "Extra-cellular matrix in vascular networks". Cell Proliferation. 37 ( ... Because only one copy of the gene is directing the cell to make pro-alpha1(I) chains, cells from people with this disorder make ... The mutations in the COL1A1 gene that cause this disorder instruct the cell to leave out a part of the pro-alpha1(I) chain that ... These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are ...
... is a selective inhibitor of breast cancer stem cells". Cell Proliferation. 44 (5): 401-9. doi:10.1111/j.1365-2184.2011.00766.x ...
Cell Proliferation. 37 (3): 221-229. doi:10.1111/j.1365-2184.2004.00307.x. PMC 6496511. PMID 15144499. Umezu, T.; Nagano, K.; ... Prashar, A.; Locke, I. C.; Evans, C. S. (2004). "Cytotoxicity of lavender oil and its major components to human skin cells". ... Ishida, Takashi (2005). "Biotransformation of Terpenoids by Mammals, Microorganisms, and Plant-Cultured Cells". Chemistry & ...
"Suppressive role of regucalcin in liver cell proliferation: involvement in carcinogenesis". Cell Proliferation. 46 (3): 243-53 ... Regucalcin can control enhancement of cell proliferation due to hormonal stimulation. Moreover, regucalcin has been shown to ... Regucalcin plays a pivotal role as a suppressor protein for cell signaling systems in many cell types. Overexpressing of ... Overexpression of regucalcin suppresses cell death and apoptosis in the cloned rat hepatoma cells and normal rat kidney ...
Doerflinger RM (February 2008). "The problem of deception in embryonic stem cell research". Cell Proliferation. 41 (Suppl 1): ... Stem-cell therapy is used to replace damaged neurons by transplantation of stem cells into affected regions of the brain. ... Inclusion bodies have been found in both the cell nucleus and cytoplasm. Inclusion bodies in cells of the brain are one of the ... This technique, where one or two cells are extracted from a typically 4- to 8-cell embryo and then tested for the genetic ...
"Optimal control problems arising in cell-cycle-specific cancer chemotherapy". Cell Proliferation. 29 (3): 117-139. doi:10.1046/ ... "How fast is repopulation of tumor cells during the treatment gap?". International Journal of Radiation Oncology, Biology, ...
A flow cytometric assay". Cell Proliferation. 28 (6): 329-36. doi:10.1111/j.1365-2184.1995.tb00074.x. PMID 7626687. S2CID ... Méhes G, Pajor L (Jun 1995). "Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. ... European Journal of Cell Biology. 75 (2): 174-83. doi:10.1016/s0171-9335(98)80059-9. PMID 9548374. Ai LS, Lin CH, Hsieh M, Li C ... The Journal of Cell Biology. 113 (4): 715-29. doi:10.1083/jcb.113.4.715. PMC 2288999. PMID 2026646. "Entrez Gene: FBL ...
... is a selective inhibitor of breast cancer stem cells". Cell Proliferation. 44 (5): 401-9. doi:10.1111/j.1365-2184.2011.00766.x ...
Cell Proliferation. 48 (1): 39-46. doi:10.1111/cpr.12152. PMC 6496444. PMID 25430589. Cowan CA, Atienza J, Melton DA, Eggan K ( ... Embryonic stem cells divide more rapidly than adult stem cells, potentially making it easier to generate large numbers of cells ... Not all stem cell research involves human embryos. For example, adult stem cells, amniotic stem cells, and induced pluripotent ... "Best Hope Lies in Private Stem-Cell Funding". Retrieved 2008-01-31. "California's Stem Cell Agency". California's Stem Cell ...
Cell Proliferation. 51 (4): e12449. doi:10.1111/cpr.12449. PMC 6528848. PMID 29484737. Ross JF, Dan EW, Kun Q, Nicholas M, ... promoting gastric cancer cells proliferation via regulation of NF-κB1". Biochem. Biophys. Res. Commun. 465 (2): 225-31. doi: ... "Downregulated Long Noncoding RNA BANCR Promotes the Proliferation of Colorectal Cancer Cells via Downregualtion of p21 ... Jiang W, Zhang D, Xu B, Wu Z, Liu S, Zhang L, Tian Y, Han X, Tian D (2015). "Long non-coding RNA BANCR promotes proliferation ...
Cell Proliferation. 39 (2): 147-55. doi:10.1111/j.1365-2184.2006.00377.x. PMC 6496865. PMID 16542349. S2CID 16515437. ... "Inhibition of the adherence of P-fimbriated Escherichia coli to uroepithelial-cell surfaces by proanthocyanidin extracts from ... displays specific cytotoxic activity against colon cancer cells". Journal of Natural Products. 75 (1): 26-33. doi:10.1021/ ...
"MicroRNA-494 suppresses cell proliferation and induces senescence in A549 lung cancer cells". Cell Proliferation. 45 (1): 32-8 ... "MicroRNA-494 downregulates KIT and inhibits gastrointestinal stromal tumor cell proliferation". Clinical Cancer Research. 17 ( ... Cell Cycle. 11 (14): 2729-38. doi:10.4161/cc.21105. PMC 3409013. PMID 22785131. Altmäe S, Martinez-Conejero JA, Esteban FJ, ... "MiR-494 is regulated by ERK1/2 and modulates TRAIL-induced apoptosis in non-small-cell lung cancer through BIM down-regulation ...
2007). "Renal differentiation of amniotic fluid stem cells". Cell Proliferation. 40 (6): 936-948. doi:10.1111/j.1365-2184.2007. ... There is some debate whether c-Kit is a suitable marker to distinguish amniotic stem cells from other cell types because cells ... Evidence in favour of their embryonic stem cell nature is the cells' ability to produce clones. The use of amniotic stem cells ... "Renal differentiation of amniotic fluid stem cells". Cell Proliferation. 40 (6): 936-948. doi:10.1111/j.1365-2184.2007.00478.x ...
Zone of cell proliferation. A little closer to the marrow cavity, chondrocytes multiply and arrange themselves into ... Blood cells that are created in bone marrow include red blood cells, platelets and white blood cells. Progenitor cells such as ... These cells give rise to other cells, including white blood cells, red blood cells, and platelets. Osteoblasts are mononucleate ... After the cells are matured, they enter the circulation. Every day, over 2.5 billion red blood cells and platelets, and 50-100 ...
Oncogenes: Stimulate cell proliferation. It is in this group where members of the RAS family are found. Oncogenes generally ... which causes abnormal cell proliferation. This could provoke a malignant tumor if combined with a separate mutation in a ... RASEF is mainly found in the perinuclear region of the cell. In addition, the protein's mid-region also seems to be involved in ... Apart from binding calcium ions in the N-terminus, RASEF plays a significant role in lung cancer cell-growth. This occurs ...
Cell phones are also very common among all three groups today. Text messaging has made cell phones particularly useful for ... Lehrerleut colonies have recently struggled with the proliferation of computers and have clamped down, so that computers are no ...
Luo, Yi; Yang, Fengxia; Mathieu, Jacques; Mao, Daqing; Wang, Qing; Alvarez, P. J. J. (2014). "Proliferation of Multidrug- ... a class of beta-lactam antibiotics that are capable of killing most bacteria by inhibiting the synthesis of one of their cell ...
... p14ARF is a protein that is a known tumor suppressor.It does this by controlling cell proliferation and cell survival, however ... In addition to promoting proliferation, SLC25A22 when expressed in vitro, progressed the cell cycle and inhibited apoptosis. It ... Chen MW, Wu XJ (January 2018). "SLC25A22 Promotes Proliferation and Metastasis of Osteosarcoma Cells via the PTEN Signaling ... PANO1 is biased to being expressed in androgen sensitive cells compared to androgen insensitive cells. A predicted 3' UTR ...
... the cells in vitro were reduced in proliferation, migration and invasive potential. So the use of a tyrosine kinase inhibitor ... Lyn and Fgr are highly expressed in malignant prostate cells compared to normal prostate cells. When the primary prostate cells ... HSP90 inhibitor NVP-BEP800 has been described to affect stability of Src tyrosine kinase and growth of T-cell and B-cell acute ... Src, Fyn and Yes are expressed ubiquitously in all cell types while the others are generally found in hematopoietic cells. c- ...
... and cell death. Daxx interacts with the TGF-β type II receptor by binding of C-terminal domain of the protein. When the cell is ... TGF-β regulates a variety of different cellular developmental processes including growth, differentiation, proliferation, ... Another important cell death-property of Daxx is the association with PML-NB. It was shown that Daxx associates with Pml only ... This partnership is found mainly in the S-phase of the cell cycle. No expression of Daxx leads to malfunction of S phase and ...
... while the forced suppression of the CMTM5 gene promote the proliferation of these cells. These studies suggest that the CMTM5 ... The forced over expression of CMTM5-v1 in Huh7 human hepatic cells also inhibited the ability of these cells to grow in a mouse ... Cai B, Xiao Y, Li Y, Zheng S (August 2017). "CMTM5 inhibits renal cancer cell growth through inducing cell-cycle arrest and ... the forced overexpression of the CMTM5 gene inhibited the proliferation and migration of cultured human endothelial cells, ...
... cell-cell adhesion and neurite outgrowth using atomic force microscopy-based single-cell force spectroscopy". Nano Letters. 13 ... The peptide cleaved from the C terminal of Ten-m3, TCAP-3, stimulates the production of cAMP and the proliferation of neurons. ... Ten-m3 mRNA is prominently co-expressed with Ten-m2 and Ten-m4 in the Purkinje's cell zone of the cerebellum. Ten-m3 protein is ... They are also expressed in some non-neuronal tissues that regulate pattern formation and sites of cell migration. Some Ten-m3 ...
... can be explained as cell loss and gliosis or a proliferation of astrocytes in damaged areas of the ... Mesenchymal stem cell therapy may delay the progression of neurological deficits in patients with MSA-cerebellar type. Ronald ... Hass EW, Sorrentino ZA, Xia Y, Lloyd GM, Trojanowski JQ, Prokop S, Giasson BI (August 2021). "Disease-, region- and cell type ... July 2012). "A randomized trial of mesenchymal stem cells in multiple system atrophy". Annals of Neurology. 72 (1): 32-40. doi: ...
... is prepared from yeast cell wall and consists of protein-carbohydrate complexes. It is used to induce experimental ... Zymosan A also raises cyclin D2 levels suggesting a role for the latter in macrophage activation besides proliferation. It ... acute liver damage after galactosamine injection suggesting that certain types of nonparenchymal cells other than Kupffer cells ...
UPS proteolysis plays a major role in responses of cancer cells to stimulatory signals that are critical for the development of ... the UPS also plays a role in inflammatory responses as regulators of leukocyte proliferation, mainly through proteolysis of ... Goff SP (Aug 2003). "Death by deamination: a novel host restriction system for HIV-1". Cell. 114 (3): 281-3. doi:10.1016/S0092- ... Kleiger G, Mayor T (Jun 2014). "Perilous journey: a tour of the ubiquitin-proteasome system". Trends in Cell Biology. 24 (6): ...
Flores ER, Halder G (2011). "Stem cell proliferation in the skin: alpha-catenin takes over the hippo pathway". Sci Signal. 4 ( ... F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by ... A tumor cell line with defective δ-catenin, low levels of E-cadherin and poor cell-to-cell adhesion could be restored to normal ... providing the cell with a means of stable cell adhesion. However, decreases in this adhesion ability of the cell has been ...
Expression of heat shock protein 70kDa protein 2 in transformed tumor cells has been implicated in the rapid proliferation, ... Molecular Cell Biology. 20 (11): 665-680. doi:10.1038/s41580-019-0133-3. PMID 31253954. S2CID 195739183. Kishor A, White EJ, ... Hsp70-2 specifically is developmentally expressed in male germ line cells during meiosis, where it is necessary for the ... June 2021). "DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network". Molecular Cell. 81 (12): ...
It forms homodimers and plays an important role in cell-cell signaling, immune responses and regulation of cell proliferation. ... Compared to wild-type naïve T cells, ICOS-/- T cells activated with plate-bound anti-CD3 have reduced proliferation and IL-2 ... It is thought to be important for Th2 cells in particular. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell ... CD4+ T cell activated in vitro reduced IL-4 secretion, while maintaining similar IFN-g secretion. Similarly, CD4+ T cells ...
... gene expression in esophageal squamous cell carcinoma cell line EC9706]". Zhonghua Zhong Liu Za Zhi (in Chinese). 33 (8): 570-3 ... "Overexpression of candidate tumor suppressor ECRG4 inhibits glioma proliferation and invasion". J. Exp. Clin. Cancer Res. 29: ... to inhibit cancer cell growth in esophageal carcinoma". BMC Cancer. 11: 52. doi:10.1186/1471-2407-11-52. PMC 3039630. PMID ... "Expression of ECRG4 is an independent prognostic factor for poor survival in patients with esophageal squamous cell carcinoma ...
Macfarlane Burnet proposed autoreactive cells would be terminated before maturation in order to prevent further proliferation ... Such T cells are often removed via clonal deletion, leaving autoreactive B cells unstimulated and unactivated. These B cells do ... Thymic dendritic cells and macrophages appear to be responsible for the apoptotic signals sent to autoreactive T cells in the ... This occurs after the functional B-cell receptor (BCR) is assembled. It is possible for B cells with high self affinity to go ...
Type II interferons are also released by cytotoxic T cells and type-1 T helper cells. However, they block the proliferation of ... A virus-infected cell releases viral particles that can infect nearby cells. However, the infected cell can protect neighboring ... and its expression is restricted to immune cells such as T cells and NK cells. All interferons share several common effects: ... They also suppress the proliferation of endothelial cells. Such suppression causes a decrease in tumor angiogenesis, a decrease ...
Bacterial replication in host cells causes endothelial cell proliferation and inflammation, resulting in mononuclear cell ... This species of Rickettsia uses an abundant cell surface protein called OmpB to attach to a host cell membrane protein called ... This causes the host cell membrane to protrude outward and invaginate the membrane of an adjacent cell. The bacteria are then ... enabling spread from cell to cell without exposure to the extracellular environment. Rickettsia rickettsii initially infect ...
... is shown to regulate neural stem cell proliferation and differentiation in mouse embryonic stem cells, and neuronal ... "miR-124 and miR-137 inhibit proliferation of glioblastoma multiforme cells and induce differentiation of brain tumor stem cells ... By inhibiting the Cdc42/PAK signalling pathway, miR-137 decreases proliferation, invasion and G0/G1 cell cycle progression of ... induces cell cycle G1 arrest and inhibits invasion in colorectal cancer cells". Int J Cancer. 128 (6): 1269-79. doi:10.1002/ijc ...
G1/S transition suggesting that p120 regulates the cell cycle and nucleolar activity that is required for cell proliferation. ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 12, All ... Cell Biol. 4 (7): 529-33. doi:10.1038/ncb814. PMID 12080348. S2CID 24923289. Holsinger LJ, Ward K, Duffield B, Zachwieja J, ... Cell. 13 (11): 4100-9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849. Beausoleil SA, Jedrychowski M, Schwartz D, Elias ...
"Data on the Hippomobile and hydrogen/fuel cells". TÜV SÜD Industrie Service GmbH. Archived from the original on 6 October 2008 ... Some particular contemporary developments are the proliferation of front- and all-wheel drive, the adoption of the diesel ... powered by non-rechargeable primary cells. In 1838, Scotsman Robert Davidson built an electric locomotive that attained a speed ... powered by non-rechargeable primary cells. In November 1881, French inventor Gustave Trouvé demonstrated a working three- ...
McDonald, Henry (2 March 2008). "MI5 targets Ireland's al-Qaeda cells". The Guardian. Retrieved 5 June 2014. Howells, Kim (May ... counter proliferation and counter espionage), Irish and domestic counter-terrorism, and technical and surveillance operations. ...
Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53-63. doi ... Amini S, Khalili K, Sawaya BE (2004). "Effect of HIV-1 Vpr on cell cycle regulators". DNA Cell Biol. 23 (4): 249-60. doi: ... Nilsson I, Hoffmann I (2000). "Cell cycle regulation by the Cdc25 phosphatase family". Progress in Cell Cycle Research. 4: 107- ... "Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe)". Bulavin, D V; Higashimoto Y; Popoff I J; Gaarde W A; Basrur V ...
"Selective inhibition of leukemia cell proliferation by BCR-ABL antisense oligodeoxynucleotides". Science. 253 (5019): 562-5. ... cell division, cell adhesion, and stress response such as DNA repair. Activity of ABL1 protein is negatively regulated by its ... a site for phosphorylation in leukaemia cells". Genes to Cells. 9 (9): 781-90. doi:10.1111/j.1365-2443.2004.00772.x. PMID ... Cell. 6 (6): 1413-23. doi:10.1016/S1097-2765(00)00138-6. PMID 11163214. Yoshida K, Komatsu K, Wang HG, Kufe D (May 2002). "c- ...
... nuclear weapons proliferation, and unresolved questions concerning nuclear waste. The organization argues that the potential of ... protecting marine biodiversity from toxins released during seabed mining for natural gas and rare metals for photovoltaic cells ...
... cell proliferation in synergy with cytokines: possible role in progenitor survival". Blood. 95 (3): 756-768. doi:10.1182/blood. ... as a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of ... Cells expressing CD34 (CD34+ cell) are normally found in the umbilical cord and bone marrow as haematopoietic cells, or in ... December 2019). "Single-cell analysis of bone marrow-derived CD34+ cells from children with sickle cell disease and thalassemia ...
Some of the most common assays are: ELISAs Protein and cell growth assays Protein:protein interactions Reporter assays Nucleic ... Mosmann, Tim (December 1983). "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and ... acid quantitation Molecular interactions Enzyme activity Cell toxicity, proliferation, and viability ATP quantification ... to look at cell populations Label-free instruments that use specialized microplates to measure binding events without the use ...
... unspecialised male germ cell) that undergoes mitotic proliferation to form primary spermatocytes (diploid - 46 chromosomes in ... Germ cell nest breakdown involves the degeneration of many germ cell nuclei and the invasion of pre-granulosa cells into the ... In the germ cell nest, one germ cell matures into an oocyte whereas others act as 'nurse cells', transferring their contents ... The interconnected oogonia are surrounded by somatic cells called granulosa cells. Later on in development, the germ cell nests ...
After establishing the key role that mast cells and other key effector cells play in triggering the acute allergic inflammatory ... mucous metaplasia and proliferation of smooth muscle. In a collaboration with Genome Therapeutics Corporation in Waltham, Mass ... This led to the subsequent discovery that epithelial cells from those with moderate-severe asthma were deficient in their ...
... performed tests on a variety of cells that carried the Tat protein and observed the rate of cell proliferation in cells ... The results of these tests showed that the amount of Tat protein within a cell infected by HIV-1 is directly correlated to the ... Wong-Staal's research focused on gene therapy, using a ribozyme "molecular knife" to repress HIV in stem cells. The protocol ... Gallo, conducted research on the human retrovirus, human T cell leukemia virus (HTLV), and determined that it was the causative ...
They have high rates of cell division and growth. ... Cancer cells are very prolific. They have high rates of cell ...
Antibodies for proteins involved in negative regulation of B cell proliferation pathways, according to their Panther/Gene ...
CD4 T-cell immunity active six months into COVID-19 convalescence A new paper explores the presence of CD4 T-cell-mediated ... New micro-protein supports cell division and proliferation during nutrient scarcity Researchers from the University of Eastern ... Neural stem cells plus HER2 inhibitor drug improve survival in mice with metastatic cancer Neural stem cells (NSCs) engineered ... In a recent study published in the latest issue of the journal Cell, a team of researchers evaluated T-cell reactivity to the ...
Id1 (inhibitor of differentiation or DNA binding protein 1) contributes to tumorigenesis by stimulating cell proliferation, i … ... However, the mechanism by which apigenin inhibits cancer cells is not fully understood. ... Apigenin inhibits proliferation of ovarian cancer A2780 cells through Id1 FEBS Lett. 2009 Jun 18;583(12):1999-2003. doi: ... contributes to tumorigenesis by stimulating cell proliferation, inhibiting cell differentiation and facilitating tumor ...
In this webinar we provide a detailed review of available methods for measuring cell proliferation, with a particular focus on ... Optimizing Cell Proliferation Studies. Tools and Techniques for Optimizing Cell Proliferation Studies ...
... epithelial cell migration and secretory cell differentiation. However, intestinal epithelial cell proliferation is not impeded ... These results suggest that ERK5 provides a common bypass route in intestinal epithelial cells, which rescues cell proliferation ... Furthermore, targeting both pathways causes a more effective suppression of cell proliferation in murine intestinal organoids ... the ERK5 pathway is upregulated to maintain epithelial cell proliferation. ...
... in ovarian cancer. ... Bufalin inhibits glycolysis-induced cell growth and proliferation in ovarian cancer. - GreenMedInfo Summary ... Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrinβ2/FAK signaling pathway ... These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through ...
EP-2046945-B1 chemical patent summary.
Cell sensitivity to chlorambucil (CLB) and fludarabine (Flu) was assessed by Cell Counting Kit-8. ,i,Results,/i,. The ... After inhibiting HuR, inflammatory response and apoptosis were significantly increased, and the cell sensitivity to CLB and Flu ... LCL lymphoblast cells and B lymphocytes were subjected to HuR overexpression (OV) or interference (IV). Western blot was used ... the proliferation of LCLs and B lymphocytes treated by CLB and Flu decreased significantly after HuR blockade (,span class= ...
Newly formed tetraploid cells can progress through one cell cycle, but the majority of cells arrest or die in the subsequent G1 ... Newly formed tetraploid cells can progress through one cell cycle, but the majority of cells arrest or die in the subsequent G1 ... Proliferation and arrest of human tetraploid cells Durch Fehler entstandene tetraploide Zellen sind chromosomal instabil und ... From the primary screen we identified 1159 genes that decreased and 431 genes that increased the cell proliferation after ...
To reveal the regulatory mechanism of proliferation in woody plant cell lines with different proliferative potential, we used ... A total of nine metabolites related to glutathione was significantly upregulated in the F cell line compared with the S cell ... sulfoximine treatment assay demonstrated the positive role of glutathione in the proliferation of Korean pine embryogenic cells ... A total of 17 glutathione-related differentially expressed genes was identified between F and S cell lines. A total of 893 ...
cell motility + cell population proliferation + The multiplication or reproduction of cells, resulting in the expansion of a ... cell proliferation involved in heart valve development + cell proliferation involved in imaginal disc-derived wing ... cell population proliferation (GO:0008283). Annotations: Rat: (2290) Mouse: (2339) Human: (2495) Chinchilla: (1940) Bonobo: ( ... Genes QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data ...
title = "Do macrophages participate in mesangial cell proliferation?",. abstract = "Mesangial cell proliferation is a harbinger ... In vitro studies support a role for macrophages in mesangial cell proliferation. Macrophage interaction with mesangial cells ... In vitro studies support a role for macrophages in mesangial cell proliferation. Macrophage interaction with mesangial cells ... In vitro studies support a role for macrophages in mesangial cell proliferation. Macrophage interaction with mesangial cells ...
... but is cell autonomously required for their proliferation and differentiation.. Germ cell specific Mtor knockout mice exhibit a ... Cell-autonomous requirement for mammalian target of rapamycin (Mtor) in spermatogonial proliferation and differentiation in the ... we created conditional germ cell knockout mice to investigate the germ cell-autonomous role of MTOR in spermatogonial ... MTOR germ cell KO mice were viable and healthy, but testes from neonatal (postnatal day (P)8), juvenile (P18), and adult (P , ...
Cell proliferation studies on these lines reveal that only wild-type coilin and the OFF mutant are sufficient to rescue the ... and are often found in cells with high transcriptional demands such as neuronal and cancer cells, but can also be observed less ... Interestingly, a stable cell line induced to express the coilin S489D phosphomutant displays nucleolar accumulation of the ... Coilin is considered to be the CB marker protein and is essential for proper CB formation and composition in mammalian cells. ...
MTT Cell Proliferation Assay Kit to quantify cell numbers based on metabolic activity in microplates. ... 1. Seed cells in a 96-well plate at a density of 104-105 cells/well in 100 µL of cell culture medium with compounds to be ... A cell titration experiment using a range of cells from 103-105 cells/well can be used to generate a standard curve ... Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and ...
Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription. Sandeep Gopal, Aqilah Amran, Andre ... Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription Message Subject (Your Name) has ... Notch receptors are essential membrane-bound regulators of cell proliferation and differentiation in metazoa. In the nematode ... Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology ...
Cell viability assay was performed in osteosarcoma cell lines 143B and U2OS. Colony formation analysis was included when cells ... The inhibitive roles of actein in cell proliferation, migration and invasion suggest that actein may serve as a potential ... Cell cycle assay was conducted to further examine the role of actein. Cell apoptotic rate and the relative activities of ... It was revealed that osteosarcoma cells were arrested in G0/G1 phase in the cell cycle progression and induced to apoptosis by ...
... , NING. ZHANG, HAIJUN. YANG* AND QINGQI. ZENG ... Hallstrom TC, Nevins JR: Balancing the decision of cell proliferation and cell fate. Cell Cycle 2009;8(4):532-5. ... C) The cell proliferation was investigated when PC3, Du145 and LNCap cells infected with lentivirus containing FOXN3 ORF. (D) ... Fig: 5: FOXN3 inhibited PC cell proliferation by repressing E2F1. (A) Effet of FOXN3 overexpression on the cell viability of ...
Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer. Cell ... Here we report the development of a novel method for the kinetic measurement of cell proliferation using the Cellometer Vision ... The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. ... for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell ...
Seminars and Events at the Research Institute of Molecular Pathology (IMP) and Vienna Biocenter (VBC).
... regulated molecular switch linking IGF signaling to epithelial cell proliferation and bone calcification. ... stress-induced NaR cell reactivation and proliferation.. To test whether the action of Papp-aa is restricted to NaR cells, we ... stress-induced NaR cell quiescence-proliferation transition, indicating the importance of local endogenous Papp-aa in NaR cells ... In both papp-aa-/- mutant and igfbp5a-/- mutant fish, the low [Ca2+] stress-induced IGF signaling and NaR cell proliferation ...
Gastric cancer cells had higher CDK4 expression than GES-1 cells. BMP-2 decreased CDK-4 expression in cancer cells but had no ... Keywords: Bone morphogenetic protein 2, Noggin, gastric cancer cell, proliferation, cell cycle, apoptosis, cyclin-dependent ... Noggin did not affect the cell cycle of GES-1 cells. The CDK4 expression was markedly increased in 2 types of cancer cells but ... Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells. Int J Med Sci. 9(2):184-192. ...
Inhibition of Cancer Cell Proliferation and Suppression of TNF-induced Activation of NFκB by Edible Berry Juice. DOMINIQUE ... Inhibition of Cancer Cell Proliferation and Suppression of TNF-induced Activation of NFκB by Edible Berry Juice ... Inhibition of Cancer Cell Proliferation and Suppression of TNF-induced Activation of NFκB by Edible Berry Juice ... Inhibition of Cancer Cell Proliferation and Suppression of TNF-induced Activation of NFκB by Edible Berry Juice ...
... ... aim of this study is to evaluate the prognostic value in GIST of some oncoproteins involved in regulation of cell proliferation ...
Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis. Submitted by ... Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis. ... Home » Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis ... development Arabidopsis Proteins/*metabolism *Cell Proliferation Cyclin-Dependent Kinase Inhibitor Proteins/*metabolism DNA ...
WST-based colorimetric measurement of cell viability for proliferation and cytotoxicity assays. CCK-8 gives us more sensitive ... Cell Proliferation / Cell Cytotoxicity Assay. Cell Proliferation / Cell Cytotoxicity Assay Kits /Related Reagents ... Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation ... Cell Staining. Cell Double Staning Kit /Live Cell Staining /Dead Cell Staining /Nuclear Staining /Mitochondria Staning /Tissue ...
amine functional groupsboron-nitride nanotubescell linescell responsecontinuous Wavecontinuous wavesgas plasma treatmentsinput ...
Untreated cells and cells that had been treated with 0.02 mg/ml β-elemene for 24, 48 or 72 h to reach ≥50% inhibition of cell ... Uncontrolled cell proliferation is the prerequisite of oncogenesis. Therefore, studies concerning tumor proliferation kinetics ... Cell culture. The bladder cancer cell line T24 was purchased from the cell repository of Xiangya Medical College of Zhongnan ... 2B and C). Cells treated for 48 h showed increased percentages of round cells, with some cells germinated along the cellular ...
The aim in this study was to explore the role of long non-coding RNA GHET1 in development of non small cell lung cancer (NSCLC ... Keywords: Non small cell lung cancer, long non-coding RNA, GHET1, cell proliferation, cell invasion ... Measuring the cell proliferation and invasion abilities by CCK8, cell colony formation and transwell invasion assays. Relative ... Knockdown of lncRNA GHET1 suppresses cell proliferation, invasion and LATS1/YAP pathway in non small cell lung cancer Article ...
  • showed that HuR inhibited tumor cell apoptosis and promoted tumor cell proliferation and migration, and that inhibition of HuR activity resulted in obvious antitumor properties [ 3 , 4 ]. (
  • Dieser neue experimentelle Ansatz ermöglichte die Identifikation von Genen, die spezifisch die Proliferation von tetraploiden Zellen verstärken oder einschränken Im Primärscreen wurden 1159 Gene identifiziert, deren Inhibition die Proliferation einschränken. (
  • Weiter wurden 431 Gene identifiziert, deren Inhibition die Proliferation der tetraploiden Zellen verstärken. (
  • Von den 431 Genen, deren Inhibition die Proliferation verstärken, wurden 371 Gene einem Konfirmationsscreen unterzogen, in dem 158 der identifizierten 371 Gene bestätigt wurden. (
  • Des weiteren wurden mittels einer Meta Analyse der Ergebnisse des Primärscreens, zusammen mit den Daten aus dem "Project Achilles", welches genomweit den Effekt von shRNA-vermittelter Geninhibition auf die Proliferation von 108 Krebszelllinien untersuchte, 18 Gene identifiziert, deren Inhibition sowohl die Proliferation von tetraploiden Zellen einschränkt, als auch die Proliferation von Zelllinien hemmt, welche von Krebsarten stammen, die zu meist chromosomale Instabilitäten (CIN) aufweisen. (
  • Since this phenotype resulted from global inhibition of mTORC1, we created conditional germ cell knockout mice to investigate the germ cell-autonomous role of MTOR in spermatogonial differentiation. (
  • BMP-2 exerted inhibitory effect on the growth of all types of cells and the inhibition become more evident with the increase of BMP-2 dose. (
  • BR inhibition of DA1 activity maintains higher levels of DA1 substrates such as UBP15 that sustain the potential for cell proliferation during leaf growth. (
  • In addition, for several mutated TTRs, the inhibition activities against the proliferation, migration and tube formation of hRECs were absent in vitro. (
  • Cell senescence via cell cycle arrest at the G1 phase was induced by NACC1 inhibition. (
  • Extremely low frequency magnetic fields regulate differentiation of regulatory T cells: potential role for ROS-mediated inhibition on AKT [med. (
  • A matched control cell standard curve using sequentially increased cell numbers was included on the plate for each corresponding cell line to determine growth inhibition. (
  • In vitro studies support a role for macrophages in mesangial cell proliferation. (
  • Forkhead box N3 inhibited prostate cancer cell proliferation in vitro and in vivo. (
  • In vitro studies suggested that PAPP-A is tethered to the cell-surface and primarily cleaves IGFBP4 and IGFBP5 among the six IGFBPs. (
  • β-elemene has been investigated in numerous studies and has demonstrated potent ability to inhibit the growth of and kill multiple types of malignant cells in vitro and in vivo ( 1 , 2 ). (
  • These findings suggested that levobupivacaine inhibits proliferation and promotes breast cancer cells apoptosis in vitro. (
  • This study investigated the role of green tea extracts as a cytotoxic agent against human ovarian cancer cells in vitro using different concentrations of green tea extracts (20, 50,100 and 250 μg mL -1 ) for 24 h. (
  • In vitro proliferation assays were performed to compare the growth rate of all groups of cells. (
  • Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro. (
  • To prove the effectiveness and feasibility of a paclitaxel hirudin complex and to provide experimental data on the prevention of restenosis, we investigated the effects of paclitaxel hirudin complexes on the growth of human coronary artery smooth muscle cells (HCASMCs) and endothelial cells (HCAECs) in vitro. (
  • [ 40 ] IL-7 and IL-4 are essential for the survival of naive CD4 + T cells [ 41 ] and, although the exact mechanisms are not well understood, some in vitro studies in humans have concluded that IL-7 and other cytokines can stimulate T-cell replication without the loss of the naive phenotype. (
  • In vitro, human gastric carcinoma SGC-7901 cells were treated with N-desulfated heparin in different concentration (0.1 mg/mL, 1 mg/mL, N-desulfated heparin group), and treated with medium (control group). (
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (
  • FK-506 is a potent immunosuppressant and in vitro T cell proliferation blocker. (
  • Although clinical trials are mostly lacking, Astragalus has been credited with immune-modulating actions via numerous molecular, cell culture, animal, and in-vitro research, including effects on T cells, T-cell receptors, and cytokines. (
  • Details] Millimeter wave induced reversible externalization of phosphatidylserine molecules in cells exposed in vitro [med. (
  • Cell viability assay was performed in osteosarcoma cell lines 143B and U2OS. (
  • Cell cycle assay was conducted to further examine the role of actein. (
  • Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. (
  • MTT assay was performed to detect the proliferation, flow cytometry done to measure the cell cycle and apoptosis and immunohistochemistry carried out to determine the expression of cyclin-dependent kinase 4 (CDK4). (
  • Only 15 minutes of handling time is needed for Cell Counting Kit-8, whereas longer handling time is required for both MTS and MTT assay. (
  • Unlike MTT assay, there is no need to lyse cells, so you don't have to worry about the data variation. (
  • CCK-8 (WST-8) is the highest sensitive dye for the cell based assay. (
  • To ensure the data is reflecting the cell death instead of decreased metabolic activity, Cytotoxicity LDH Assay Kit-WST (CK12) is used to increase the data reliability by measuring LDH released from dead cells. (
  • Results of a methylthiazolyl tetrazolium (MTT) assay indicated that the proliferation of T24 cells was repressed by β-elemene in a time- and concentration‑dependent manner. (
  • Results Colony formation and transwell assay were used to determine breast cancer cells proliferation, whereas flow Cytometry (annexin V and PI staining) was used to investigate breast cancer cells apoptosis. (
  • Through CCK-8 assay, we determined the MCF-7 and MDA-MB 231 cells viability. (
  • A549 cells were treated with different concentrations of formononetin, then detected the cell proliferation, apoptosis and the expression of HIPK2 respectively by MTT assay, flow cytometry analysis and RT-qPCR. (
  • The MTS assay revealed that cell proliferation was significantly reduced after transient transfection of miR-331-3p precursor and/or NACC1 siRNA in UC cells. (
  • MTT cytotoxicity assay demonstrates that our phyto-synthesized ZnFe 2 O 4 nanoparticles exhibited a selective and potent anticancer activity against K562 and MDA-MB-231 cell lines with IC50 values 4.53 μM and 4.19 μM, respectively. (
  • In order to assess the cytotoxicity of eluates of the studied materials obtained after 1 hour , 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. (
  • Proliferation was measured by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) Cell Proliferation Assay, according to the manufacturer's protocol (ATCC). (
  • Here we show that loss of Erk1 / 2 in intestinal epithelial cells results in defects in nutrient absorption, epithelial cell migration and secretory cell differentiation. (
  • The inhibitive roles of actein in cell proliferation, migration and invasion suggest that actein may serve as a potential therapeutic agent in the treatment of osteosarcoma. (
  • Laminin bound to HSC supports proliferation and migration of these cells. (
  • From the collagen family the collagen type IV, especially denatured collagen IV, was shown to stimulate secretion of matrix metalloproteinase 9 (MMP-9) and regulate migration of cells in bone marrow, which may promote leukaemogenesis due to increased activation of AKT via LSC interaction [5]. (
  • Transthyretin affects the proliferation and migration of human retinal microvascular endothelial cells in hyperglycemia via hnRNPA2B1. (
  • Aside from its anticoagulant action, heparin binds to various growth factors, cytokines, and extracellular proteins and consequently is able to affect migration of cancer cells and angiogenesis in tumors. (
  • It inhibits endothelial cell proliferation and migration [5]. (
  • Moreover, transwell assays were used to explore the effects of actein on cell metastasis. (
  • Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. (
  • Measuring the cell proliferation and i nvasion abilities by CCK8, cell colony formation and transwell invasion assays. (
  • Here, we show that an HBZ-miRNA axis promotes cell proliferation and genetic instability, as indicated by comet assays that showed increased numbers of DNA-strand breaks. (
  • Cell viability was assessed using methylthiazoletetrazolium (MTT) assays to determine the optimal concentration range for inhibiting the growth of HCASMCs but not that of HCAECs. (
  • Both compounds at a concentration of 5muM lowered cell viability and proliferation, with EGCG being more effective than SU11274, and the invasion of colon cancer cells in Matrigel assays was strongly inhibited. (
  • MAPKs are involved in regulation of mitosis, gene expression, cell metabolism, cell motility and apoptosis. (
  • Here, we sought to elucidate the regulatory function of bufalin on cell glucose metabolism in ovarian cancer. (
  • The forkhead box family has been implicated in a broad spectrum of cellular processes, metabolism, DNA repair, differentiation, including cell proliferation, and aging[ 7 ]. (
  • To understand the mechanism of glutamine-stimulated proliferation, we analyzed published transcriptomics data for enzymes involved in the glutamine metabolism pathway and found 3-fold greater gene expression of glutaminase (Gls/GLS) in mouse and human alpha cells than in other pancreatic endocrine cells, while Gls2/GLS2 expression was extremely low in islet cells. (
  • Within islets, both human and mouse alpha cells had higher expression than beta cells, suggesting that alpha cells may have increased capacity for glutamine metabolism. (
  • These data indicate that glutamine metabolism through GLS is critical for amino acid-dependent alpha cell proliferation in mice and support that alpha cells are unique amino acid sensors playing a critical role in amino acid homeostasis. (
  • CcRCC is associated with the reprogramming of fatty acid metabolism , and stearoyl- CoA desaturases (SCDs) are the main enzymes controlling fatty acid composition in cells . (
  • We have now realized that the combined action of both hyperoxia (an excess of oxygen in the body) and hyperbaric pressure, leads to significant improvement in tissue oxygenation while targeting both oxygen and pressure sensitive genes, resulting in improved mitochondrial metabolism with anti-apoptotic (anti-cell death) and anti-inflammatory effects. (
  • My current research interest is T cell metabolism. (
  • mTOR, a serine/threonine kinase, is thought to play a central role in regulating cell growth, proliferation, cellular metabolism and angiogenesis [ 7 ]. (
  • We demonstrate the importance of employing the correct cell cycle time distribution by recapitulating the results from two models incorporating cellular proliferation (one spatial and one non-spatial) and demonstrating that changing the cell cycle time distribution makes quantitative and qualitative differences to the outcome of the models. (
  • Our adaptation will allow modellers and experimentalists alike to appropriately represent cellular proliferation-vital to the accurate modelling of many biological processes-whilst still being able to take advantage of the power and efficiency of the popular Gillespie algorithm. (
  • With the in-depth study, it was found that the E2F family played an key role in the process of cell growth, differentiation and apoptosis[ 12 ]. (
  • To investigate the effects of bone morphogenetic protein-2 (BMP-2) on the proliferation, differentiation and apoptosis of normal human gastric mucosal cells and gastric cancer cells. (
  • The Protein kinase C (PKC) -associated sign pathway performs essential roles in regulation of cell development, differentiation and apoptosis. (
  • Also known as transcriptional activators for their ability to induce transcription of genes into RNA messages, these proteins are essential for the cells to function properly. (
  • Yet little is known about these proteins, and it wasn't clear how many activators there might be in human cells - until now. (
  • In a recent study published in the latest issue of the journal Cell, a team of researchers evaluated T-cell reactivity to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant's spike (S) and non-spike proteins. (
  • These proteins are typically involved in the regulation of cell proliferation-more specifically, G1/S-phase cell cycle progression-and differentiation. (
  • The basic role of these proteins is the promotion of proliferation, maturation, and differentiation of haematopoietic stem cells (HSC) with nutrient-rich microenvironment and growth factors. (
  • It is noteworthy that, from ECM proteins, collagens and laminin enhance the proliferation and differentiation of myeloid cells not only in bone marrow but also in the extramedullary localisation [9]. (
  • The CCL2 in MAC-T cells attenuates endoplasmic reticulum stress induced by tunicamycin, suggesting that CCL2 regulates intracellular synthesis of proteins and lipids and prevents activation of apoptotic pathways initiated in response to endoplasmic reticulum stress. (
  • Previous reports suggest that electrical forces on cell structure proteins interfered with the chromosome separation during mitosis and induced apoptosis. (
  • Because of their surface charge, asbestos fibers can adsorb to cellular macromolecules (proteins, DNA, RNA) and cell surface proteins. (
  • Your immune system is a complex network of cells, proteins, tissues and organs that work together to protect you against germs, infectious organisms and disease. (
  • β-elemene, a non-cytotoxic antitumor reagent, inhibits the growth, proliferation and DNA synthesis of multiple types of malignant cells, resulting in the apoptosis or suppressed vascularization of tumors. (
  • microRNA (miRNA) constitutes a class of small non-coding RNAs, which are involved in cell proliferation, differentiation, and progression of tumors. (
  • NETs are a group of tumors with heterogenous malignancy that evolve from neuroendocrine cells, with the lung being the second target organ after the gastrointestinal tract. (
  • Lung neuroendocrine tumors (LNETs) are a group of rare tumors with heterogenous malignancy originating in amine precursor uptake and decarboxylation (APUD) neuroendocrine cells from Kulchitsky cells (argentaffin cells) ( 1 ). (
  • Azzopardi introduced the term of oat cell carcinoma in 1959 after a study on 100 cases of pulmonary tumors ( 4 ). (
  • The neuroendocrine cells from which neuroendocrine tumors (NETs) derive are located in numerous places in the human body ( 8 ). (
  • In this study we investigated whether Notch signaling is involved in the tight interactions between neoplastic plasma cells and their bone marrow microenvironment, which are essential for tumor cell growth in multiple myeloma (MM). Here we demonstrate that Notch receptors and their ligand Jagged1 are highly expressed in cultured and primary MM cells, whereas nonneoplastic counterparts show low to undetectable levels of Notch. (
  • By contrast, studies have showed that E2F1 inducing apoptosis is a kind of protective mechanism against tumor cell proliferation[ 16 ]. (
  • Vascular niches contain mesenchymal stromal cells, endothelial cells, and perivascular stromal cell [2]. (
  • The interaction between TTR and a target in nucleus of human retinal microvascular endothelial cells (hRECs), heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1, was screened by immunoprecipitation (IP) and mass spectrum (MS), and it was further confirmed by co-immunoprecipitation (co-IP). (
  • Actein significantly inhibited osteosarcoma cell viability in a time- and dose-dependent manner. (
  • Induction of apoptosis was confirmed by cell viability, morphological changes showed cell shrinkage, rounding, and detachments from plates. (
  • BMP-2 may inhibit the proliferation of both normal and malignant gastric epithelial cells, down-regulate CDK4 expression in gastric cancer cells and arrest gastric cancer cells in G1-phase in cell cycle. (
  • Our results revealed that β-elemene was able to upregulate the expression of Smad4 in tumor cells to inhibit the proliferation of these cells. (
  • However, the interrelation of the ERK1/2 module relative to other signalling pathways in intestinal epithelial cells and colorectal cancer (CRC) is unclear. (
  • Furthermore, targeting both pathways causes a more effective suppression of cell proliferation in murine intestinal organoids and human CRC lines. (
  • Despite vast literature on the role of ERK1/2 in cell proliferation, the absolute requirement of this signalling module in rapidly dividing tissues relative to other signalling pathways is unknown. (
  • Cell signaling pathways elicited by asbestos. (
  • Overexpressed CCL2 binding to its receptor C-C chemokine receptor 2 increases the risk of breast cancer in humans, but its effects on proliferation of bovine mammary epithelial cells is not known. (
  • Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells through PI3K/Akt/mTOR signalling pathway. (
  • SCD5 regulation by VHL depends on HIF, and loss of SCD5 promotes cell proliferation and a metabolic shift towards ceramide production . (
  • it has been hypothesized that dietary calcium may increase prostate cancer risk by reducing circulating levels of 1,25-dihydroxyvitamin D (1,25[OH] 2 D) (10), which promotes the differentiation and inhibits the proliferation of prostate cells (11). (
  • HuR participates in the regulation of biological activities and is an important mediator of cell division, cell senescence, immune cell activation, and other vital activities closely related to inflammation and tumorigenesis [ 1 , 2 ]. (
  • The presence of asbestos fibers in the lungs sets off a variety of responses leading to inflammation, cell, and tissue damage, which can lead to malignant and non-malignant diseases. (
  • Other cell-mediated mechanisms (especially inflammation). (
  • Other cell-mediated mechanisms (especially inflammation induced by TNF-α). (
  • Th2 cells are critical in maintaining both the state of chronic and relapsing eosinophil-predominant inflammation and the acute hypersensitivity responses characteristic of the atopic diseases. (
  • However, the mechanism by which apigenin inhibits cancer cells is not fully understood. (
  • Our results may elucidate a new mechanism underlying the inhibitory effects of apigenin on cancer cells. (
  • We recently demonstrated that Notch signaling is involved in proliferation and survival of B cell-derived tumor cells of classic Hodgkin disease and described a novel mechanism for the oncogenic capacity of Notch. (
  • The aim of the present study was to investigate the anti-tumor effects of formononetin on human nonsmall cell lung cancer (NSCLC) and its potential molecular mechanism. (
  • However, the molecular mechanism by which it inhibits cell proliferation remains unknown. (
  • Therefore, homeostasis of the T-lymphocyte subpopulation is dependent on a mechanism of self-regulation that consists of the homeostatic proliferation of peripheral naive T cells: the organism 'senses the space' left by the contracted T-cell compartment and attempts to restore homeostasis through the proliferation of peripheral naive T cells. (
  • In the present report we evaluate electromagnetic exposure of cells in telophase/cytokinesis in order to further analyse the mechanism of action on cells. (
  • A potential mechanism for the role of calcium in prostate cancer development and progression is that intracellular calcium controls the growth of prostate cancer cells and the process of apoptosis (9). (
  • Neuronal degeneration, edema, glial cell proliferation and perivascular infiltration (7). (
  • The histopathology reveals perivascular proliferation of spindle cells, and adjacent lymphocytic infiltrates. (
  • Neural stem cells (NSCs) engineered by Northwestern Medicine investigators used in combination with the HER2 inhibitor drug tucatinib improved survival in mice with HER2-positive breast cancer brain metastases, according to findings published in Proceedings of the National Academy of Sciences. (
  • Lgr5+ intestinal stem cells at the intestinal crypt base produce transit-amplifying cells, which then undergo a number of proliferative cycles before terminal differentiation into absorptive enterocytes at the crypt-villus border. (
  • Leukemia is a malignant clonal disease of hematopoietic stem cells, with fever, anemia, and lymphadenopathy as its main clinical symptoms. (
  • Spermatogonial stem cells must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. (
  • Notch receptors expressed on hematopoietic stem cells interact with their ligands on bone marrow stromal cells and thereby control cell fate decisions and survival. (
  • Leukaemia initiating cells, similarly to normal haematopoietic stem cells, are dependent on signals coming from cells present in both type of niches and from their soluble products [5]. (
  • The self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. (
  • Spermatogonial stem cells were established from 6-day-old male F1 progeny of DBA/2 ´ C57BL/6 or C57BL/6/Tg14 (act-EGFP-OsbY01) mice as described (17,18). (
  • The concept is a biphasic construct consisting of a porous, osteoconductive chitosan-calcium phosphate scaffold supporting a layer of neocartilage formed by marrow-derived mesenchymal stem cells. (
  • The first experiment characterized the attachment efficiency and proliferation of primary human marrow-derived mesenchymal stem cells seeded relatively sparely onto the scaffold's surface. (
  • Our goal is to create a bilayered construct which consists of an osteoconductive chitosan-calcium phosphate scaffold underlying a layer of neocartilage formed bymesenchymal stem cells (MSCs) through self-assembly. (
  • [ 26 , 27 ] One of the mechanisms is the reduced capacity to generate lymphoid progenitors by functionally impaired hematopoietic stem cells due to a deficiency in the capacity to repair DNA damage. (
  • This, he said "induces stem cells proliferation and mobilization, leading to the generation of new blood vessels (angiogenesis) and tissue regeneration. (
  • Epithelial-mesenchymal transition (EMT) and most cancers stem cells (CSCs) play a vital function in metastasis of papillary thyroid most cancers (PTC). (
  • Onuoha's experiment will explore the possibility that telomere lengthening is caused by a space-induced proliferation of stem cells-undifferentiated cells from which specialized body components arise and which typically have long telomeres. (
  • The ERK1/2 MAPK signalling module integrates extracellular cues that induce proliferation and differentiation of epithelial lineages, and is an established oncogenic driver, particularly in the intestine. (
  • Research indicates the potential for MLR-1023 to induce proliferation of a patient's remaining beta cells. (
  • Acute lymphoblastic leukaemia cells are B lymphocyte precursors of leukaemia (B-ALL), in the majority cases in a stage of differentiation and maturation when CD10 (common ALL) antigen is expressed [1]. (
  • Natural killer cell maturation and function in C57Bl/6 mice is altered by caloric restriction. (
  • IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. (
  • In the large-scale breeding of conifers, cultivating embryogenic cells with good proliferative capacity is crucial in the process of somatic embryogenesis. (
  • In the same cultural environment, the proliferative capacity of different cell lines is significantly different. (
  • This dual monoubiquitylation-phosphorylation regulation supports the key role of BR levels in maintaining the proliferative potential of cells during organ growth. (
  • During eye development, retinal progenitors are drawn from a multipotent,proliferative cell population. (
  • Together, these results implicate meis1 as a positive cell cycle regulator in early retinal cells, and provide evidence of an evolutionary conserved function for Hth/Meis genes in the maintenance of the proliferative, multipotent cell state during early eye development. (
  • In humans, an increased proliferation of CD4 + T lymphocytes has been reported in individuals above 65 years of age and thymectomized children, [ 38 , 39 ] and it is believed that the partial lymphopenia due to the loss of thymus function is responsible for the proliferative increase. (
  • Maintaining a high level of proliferative activity in bovine mammary epithelial cells during lactation is important for improving milk yield and can benefit the dairy industry economically. (
  • To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. (
  • We and others have shown that the immune response to influenza vaccine is reduced in the elderly as evidenced by lower antibody titers, decreased T cell proliferative responses, reduced cytotoxic T cell activity, and altered cytokine production compared to young controls. (
  • In the present study, we demonstrated that CCL2 induces proliferation of MAC-T cells, a bovine mammary epithelial cell line, and stimulates progression of the cell cycle through stimulation of expression of cyclin D1. (
  • Details] Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells [med. (
  • My research is focused on understanding the interplay between metabolic reprogramming and T cell differentiation to enhance anti-tumor immune response. (
  • At a cellular level, the immune system attacks pancreatic beta cells that produce insulin, leaving the patient with deficient levels of insulin to control blood glucose and a resulting dependency on exogenous insulin. (
  • Your immune system can tell the difference between healthy and unhealthy cells, and when it receives a "danger" alert, it activates what's called the immune response-a series of actions set forth by the immune system control center to attack infectious invaders and restore homeostasis. (
  • Even certain cancers, such as leukemia, are spawned by the uncontrolled proliferation of immune cells! (
  • In fact, 70% of immune system cells make up the wall of the gut, which is why the health of your gut, along with what you eat, has such a strong impact on your immune response. (
  • According to a recent study published in the Journal of Leukocyte Biology , omega-3-an essential fatty acid the body can't produce on its own but needs from your diet-helps to support and strengthen the function of the immune cells. (
  • Multiple myeloma (MM) is an incurable cancer in which uncontrolled plasma cell proliferation disrupts the bone marrow environment and impairs immune function. (
  • 10 Astragalus polysaccharides are also shown to promote proliferation and function of intestinal intraepithelial T cells - a group of specialized T cells in the gastrointestinal mucosa that may also have systemic immune modulating effects. (
  • As the millennium draws to a close, it is clear that IgE production represents only one feature of a larger specific immune response orchestrated by the Th2 subset of CD4 + Th cells. (
  • The outcome of the ensuing battle will determine whether the infection will remain locally limited within the engulfing cells of the innate immune system, or will continue to spread, causing the individual to become a clinically active TB patient [ 1 , 6 , 7 , 8 ]. (
  • Cancer cells are very prolific. (
  • Here, we demonstrate that apigenin inhibits proliferation and tumorigenesis of human ovarian cancer A2780 cells through Id1. (
  • Bufalin inhibits glycolysis-induced cell growth and proliferation in ovarian cancer. (
  • Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrinβ2/FAK signaling pathway in ovarian cancer. (
  • The treatment of bufalin on ovarian cancer cells effectively inhibited glucose uptake and lactate production in ovarian cancer cells. (
  • Mechanistically, bufalin exerted its anti-tumor effect by targeting ITGB2/FAK signaling pathwayand, which could be rescued by the introduction of ITGB2 cDNA in ovarian cancer cells. (
  • These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through repression of the ITGB2/FAK pathway, indicating that bufalin may be developed as a chemotherapeutic agent to treat ovarian cancer. (
  • In addition, studies have shown that FOXN3 is significantly downregulated in several cancer tissues including oral laryngeal carcinoma hepatocellular carcinoma, squamous cell carcinoma and colon cancer[ 6 , 9 , 10 ]. (
  • Zhang J, Ge Y, Sun L, Cao J, Wu Q, Guo L, Wang Z. Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells. (
  • Poorly differentiated gastric cancer BGC823 cells, moderately differentiated gastric cancer cells and normal human gastric mucosal epithelial GES-1 cells were independently treated with recombinant human BMP-2 or its inhibitor Noggin. (
  • After treatment with 200 ng/ml BMP-2, cancer cells arrested in G1 phase and those in S phase reduced. (
  • Gastric cancer cells had higher CDK4 expression than GES-1 cells. (
  • BMP-2 decreased CDK-4 expression in cancer cells but had no influence in GES-1 cells. (
  • In 2 types of cancer cells, treatment with 2000 ng/ml Noggin significantly increased the proportion of cells in S phase but reduced that in G1 phase. (
  • The CDK4 expression was markedly increased in 2 types of cancer cells but that of GES-1 remained unchanged after treatment with 2000 ng/ml Noggin. (
  • Through antagonizing BMP-2, Noggin, may accelerate the proliferation of gastric cancer cells. (
  • Microscopic observation also demonstrated the potential of β-elemene to induce the apoptosis of cancer cells. (
  • Our study found that β-elemene effectively inhibited the proliferation of T24 cells in a concentration- and time-dependent manner, and induced the apoptosis of T24 cells by upregulating the expression of the Smad4 gene, which provided a theoretical basis for the use of β-elemene in the treatment of bladder cancer. (
  • The bladder cancer cell line T24 was purchased from the cell repository of Xiangya Medical College of Zhongnan University. (
  • BACKGROUND: The aim in this study was to explore the role of long non-coding RNA GHET1 in development of non small cell lung cancer (NSCLC). (
  • The results of the flow Cytometry confirmed that levobupivacaine inhibited breast cancer cell proliferation and enhanced apoptosis of breast cancer cells. (
  • The effect of levobupivacaine on breast cancer cells is yet to be determined. (
  • The present study aimed to investigate the anti-tumour effects of levobupivacaine on breast cancer cells. (
  • We acquired MCF-7 and MDA-MB231 breast cancer cells from the ATCC (Beijing Zhongyuan limited, China). (
  • In particular, processes in which cell proliferation is important (e.g. embryonic development, cancer formation) should not be simulated naively using the Gillespie algorithm since the history-dependent nature of the cell cycle breaks the Markov process. (
  • The results of statistical analysis revealed significant decrease in the number of treated human ovarian cancer cells inversely proportion with increasing the concentration of green tea extracts, this experiments determined that the IC 50 = 100 μg mL -1 . (
  • Morphologically, the images of the fixed and stained human ovarian cancer cells with Coomassie stain revealed that the Green tea extracts causing cells shrinkage, blabbing, chromatin condensation and loss of cell-cell contacts which were known as sign of apoptosis. (
  • Epigallocatechin-3-gallate inhibits Met signaling, proliferation, and invasiveness in human colon cancer cells. (
  • We compared the tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) and a specific Met inhibitor, SU11274, as suppressing agents of Met signaling in HCT116 human colon cancer cells. (
  • We previously reported that NACC1 is the target molecule of miR-331-3p and is associated with cell proliferation in prostate and cervical cancer. (
  • Various analytic methods were applied for the characterization of the phyto synthesized ZnFe 2 O 4 , and they were tested for their anticancer activity against MDA-MB-231, K562, MCF-7 cancer cell lines, and normal fibroblasts. (
  • Likewise, hypoxic breast cancer cells showed higher propensity to increase expression of oncogenes and to decrease expression of tumor suppressor genes ( Jefford and Irminger-Finger, 2006 ). (
  • Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of renal cancer , and inactivation of the VHL tumor suppressor gene is found in almost all cases of hereditary and sporadic ccRCCs. (
  • This may have an important impact in understanding the biology of wild-type p53 in cancer-transformed cells. (
  • p53 is a key protein that is altered in all cancer cells. (
  • Curcumol reduced the proliferation of breast cancer cells by targeting NCL/ERα36 and inactivating the PI3K/AKT pathway' - See curcumin at . (
  • Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis - Phytother Res 2022 Sep 9 - 'Taken together, our study provided novel insight into the anticancer effects of Resv and revealed that targeting the SIRT3/AMPK/autophagy pathway can serve as a new therapeutic target against breast cancer' - See resveratrol products at . (
  • Resveratrol (Res) exerts inhibitory effects on breast cancer cell lines and animal models, while its efficacy against individual breast cancer cases remains unknown. (
  • Cancer refers to a group of diseases characterized by abnormal cell proliferation with a tendency to invade adjacent tissues and produce metastases. (
  • Effect of 10.5 GHz CW radiofrequency radiation exposure on normal and prostate cancer cell morphology [med. (
  • ECM components are important factors in functional network harmonising self-renewal of HSC, regulating cell adhesion, inflammatory response, angiogenesis, and homing of cells, e.g. tumour metastasis. (
  • Tumour-treating fields (TTFields) use alternating electric fields which interfere with dividing cells, thereby reducing tumour growth. (
  • Blood exosomes assist regulate communication between tumour cells, moderating their behaviour. (
  • Erroneously arising tetraploid mammalian cells are chromosomally unstable and may facilitate cell transformation. (
  • An increasing body of evidence suggests that the propagation of mammalian tetraploid cells is limited by a p53-dependent arrest, however, the triggers of this arrest have thus far not been identified. (
  • Moreover, we demonstrated that knockdown of lncRNA GHET1 suppresses LATS1/YAP pathway signaling pathway by downregulating YAP1 expression in NSCLC cells. (
  • We demonstrate a noncanonical nuclear receptor pathway in which RORα binds to E2F1 to inhibit cell cycle progression. (
  • De novo synthesis and salvage pathway coordinately regulates polyamine homeostasis and determines T cell proliferation and function. (
  • Intermittent pulsed electromagnetic field stimulation activates the mTOR pathway and stimulates the proliferation of osteoblast-like cells [med. (
  • Cells were dually analyzed by BD LSR II flow cytometer and BD Pathway 855 bioimaging confocal system and images merged using BD Attovision™ Software (BD Biosciences, San Jose, CA, USA). (
  • Tumor cells are monoclonal B lymphocytes with similar morphology to that of normal mature small lymphocytes and mainly exist in blood, bone marrow, and lymphoid tissue, with poor prognosis. (
  • The regulation of B-cell ontogeny in bone marrow, like other cell lines, is based on morphological structures, e.g. niches with stromal cells ("osteoblastic") and vascular niches localised at the sinusoidal walls. (
  • In B-ALL homing of leukaemia cells in bone marrow is supported by the CXCL12/CXCR4 axis because CXCR4 is present on leukaemia cells. (
  • Differentiation-Associated Expression of Conventional Protein Kinase C Isoforms in Primary Cultures of Bone Marrow Cells Induced by M-CSF and G-CSF. (
  • The current research focuses on typical PKC (cPKC) expression and its regulation in major cultures of bone marrow cells induced to endure macrophage/granulocyte differentiation by macrophage colony-stimulating issue (M-CSF) or granular colony-stimulating issue (G-CSF). (
  • Silencing RORα in mammary epithelial cells significantly enhanced cell proliferation in the ductal epithelial cells and promoted side branching of the mammary ducts. (
  • Collectively, CCL2 is a novel target for improving the quantity and quality of milk from cows through stimulation of proliferation on mammary epithelial cells and attenuation of LPS-induced inflammatory responses. (
  • Cells were then stained with a trypan blue solution (0.04% w/v, Invitrogen), and counted on a Cellometer Vision automated cell counter (Nexcelom Bioscience, Lawrence, MA, USA) according to the manufacturer's protocol. (
  • Since Cell Counting Kit-8 measures the metabolic activity of living cells, the data does not specifically verify cell death. (
  • Xuyong's research focus on T cells metabolic reprogramming, cell cycle and DNA damage. (
  • During T cell activation and differentiation, the metabolic file is dramatically switched which helps the T cell better achieve its physiological function. (
  • By deciphering the metabolic reprogramming during T cell activation and differentiation, I hope to apply my knowledge to translational use and combat autoimmune disease. (
  • John's research interests include integration of metabolic and cell cycle signaling. (
  • Understanding the Warburg effect: the metabolic requirements of cell proliferation. (
  • Reduced BR levels lead to activation of DA1 peptidase activity and a transition from cell proliferation to cell growth and differentiation. (
  • The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. (
  • Actein inhibits tumor growth by inducing cell apoptosis in osteosarcoma. (
  • Retinoic acid receptor-related orphan nuclear receptor alpha (RORα) is a potent tumor suppressor that reduces cell proliferation and inhibits tumor growth. (
  • It is, therefore, important to identify the factors that promote mesangial cell proliferation in order gain a better understanding of the progression of glomerular disease. (
  • It was revealed that osteosarcoma cells were arrested in G0/G1 phase in the cell cycle progression and induced to apoptosis by administration of actein. (
  • These results reveal a novel link between RORα and E2F1 in regulating cell cycle progression and mammary tissue morphogenesis. (
  • Aim: The present study is aimed at comparing and correlating the mast cell density (MCD) and micro vascular density (MVD) in normal mucosa and different grades of OSMF and to analyze their role in the disease progression. (
  • Genomic instability is characterized by an elevated propensity of alterations in the genome throughout the cell cycle, where coordinated cell cycle progression and error-free repair of DNA damage are crucial for maintaining genomic integrity. (
  • LCL lymphoblast cells and B lymphocytes were subjected to HuR overexpression (OV) or interference (IV). (
  • In this study, we investigated the interaction between TNF- α and HuR by exploring the effect of TNF- α on the apoptosis and inflammatory response of lymphoblast cells and B lymphocytes after inhibiting the expression of HuR. (
  • The lymphoblast cell line LCLs and B lymphocytes were obtained from the American Type Culture Collection (Virginia, USA) and cultured in RPMI 1640 medium (SH30809.01B, Hyclone, Utah, USA) supplemented with 10% fetal bovine serum (10270-106, Gibco, CA, USA) in an atmosphere containing 5% CO 2 and 95% air at 37°C for 24 hours. (
  • abstract = "Mesangial cell proliferation is a harbinger of glomerulosclerosis, leading to end-stage renal failure. (
  • abstract = "Since the use of impedance measurements for label-free monitoring of cells has become widespread but still the choice of sensing configuration is not unique though crucial for a quantitative interpretation of data, we demonstrate the application of a novel custom multipotentiostat platform to study optimal detection strategies. (
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (
  • The effect of asbestos (1332214) on apoptosis and proliferation in mesothelial cells was examined using novel cell imaging techniques. (
  • SCD5 Regulation by VHL Affects Cell Proliferation and Lipid Homeostasis in ccRCC. (
  • The aim of this study is to evaluate the prognostic value in GIST of some oncoproteins involved in regulation of cell proliferation. (
  • Detailed microarray analysis of the fibroblast genes indicated that the cell population of the third passage exhibited the highest number of upregulated genes involved in the cell cycle and cell proliferation. (
  • Importantly, RORα levels were increased in mammary ducts compared to terminal end buds and inversely correlated with expression of E2F1 target genes and cell proliferation. (
  • Newly formed tetraploid cells can progress through one cell cycle, but the majority of cells arrest or die in the subsequent G1 stage, with the fate of these tetraploid cells determined by the preceding mitosis. (
  • Daughter cells arising from defective mitosis accumulated p53 in the nucleus, which led to irreversible cell cycle arrest or death. (
  • The results showed that formononetin significantly inhibited the proliferation and induced the apoptosis of A549 cells in a time- and dose-dependent manner. (
  • Immunohistochemistry with TUR-Bt specimens revealed that greater than 90% of both UC and normal urothelial cells were positive for NACC1 in contrast to no or limited expression in squamous cell carcinoma of the esophagus, cervix, and oral cavity. (
  • We sought to find out the protein content material in serum exosomes (SEs), to characterise SEs, and to find novel scientific biomarkers of oral squamous cell carcinoma (OSCC). (
  • Gould introduced the concepts regarding multidirectional differentiation of neuroendocrine cells that can evolve in mucus-producing cells, squamous cells and pulmonary carcinomas. (
  • Id1 (inhibitor of differentiation or DNA binding protein 1) contributes to tumorigenesis by stimulating cell proliferation, inhibiting cell differentiation and facilitating tumor neoangiogenesis. (
  • Here we report the development of a novel method for the kinetic measurement of cell proliferation using the Cellometer Vision system. (
  • However, interrupted glucagon signaling increases serum amino acid and glucagon levels and stimulates amino acid-dependent alpha cell proliferation as part of an endocrine feedback loop, the liver-alpha cell axis. (
  • Because Arf stimulates PLD1, we looked for the presence of Arf in the active RalA-PLD1 complexes isolated from v-Src- and v-Ras-transformed cell lysates. (
  • Rapamycin inhibits protein kinase C activity and stimulates Na+ transport in A6 cells. (
  • MLR-1023, a lyn kinase activator with potent beta cell proliferation activity, has demonstrated exceptional clinical safety and tolerability in over 700 patients in Phase 2a and Phase 2b Type 2 diabetes studies. (
  • Microscopic examination was important because often a cell with two markers may lead the researchers to think it was a new cell. (
  • Knockdown of lncRNA GHET1 suppressed cell proliferation and invasion capacity and Epithelial-Mesenchymal Transition (EMT) phenomenon of NSCLC cells. (
  • Nucleus accumbens-associated protein 1 (NACC1), one of several transcription factors, is constitutively expressed in the urothelium, wherein it regulates cell growth, senescence, autophagy, epithelial-mesenchymal transition. (
  • The small intestinal epithelium is particularly suitable to address this question given the short (4-8 days) and dynamic life cycle of intestinal epithelial cells (IECs). (
  • A previous study showed that FOXN3 could down-regulate E2F5 in human cells to control cell cycle or inhibit protein biosynthesis[ 6 ]. (
  • In our research, cell cycle-specific transcription factor, E2F1 was identified as a downstream gene of FOXN3. (
  • Additionally, E2F family can regulate the cell cycle G1 to S phase transition[ 14 ]. (
  • Ionocytes, which are normally quiescent, re-enter the cell cycle under low [Ca 2+ ] stress. (
  • However, Noggin did not affect the cell cycle of GES-1 cells. (
  • The variance in experimentally measured cell cycle times is far less than in an exponential cell cycle time distribution with the same mean.Here we suggest a method of modelling the cell cycle that restores the memoryless property to the system and is therefore consistent with simulation via the Gillespie algorithm. (
  • By breaking the cell cycle into a number of independent exponentially distributed stages, we can restore the Markov property at the same time as more accurately approximating the appropriate cell cycle time distributions. (
  • The aim of this study was to determine the global transcriptome profile of three passages of dermal autologous fibroblasts from a male volunteer, focusing on the processes of the cell cycle and cell proliferation status to estimate the optimal passage of the tested cells with respect to their reimplantation. (
  • This role in cell cycle control is mediated by meis1 regulating cyclin D1 and c-myc transcription. (
  • Cell proliferation / International Cell Cycle Society. (
  • As a consequence of homeostatic proliferation, the naive cells produced by the thymus have a long life cycle, remaining viable in a quiescent state in the peripheral blood. (
  • These changes and markers of genetic instability are driven by a failure of DNA repair systems and cell cycle regulation. (
  • DNA-damage, cell cycle arrest and apoptosis induced in BEAS-2B cells by 2-hydroxymetyl methacrylate (HEMA). (
  • This leads skin cells to renew their cell cycle of proliferation and differentiation. (
  • Any process that stops, prevents or reduces the frequency, rate or extent of mesenchymal cell proliferation involved in lung development. (
  • Therefore, a major emphasis of our research is to study the response of young and aged mice to primary influenza infection at the site of infection, i.e., the lung, with a particular interest in NK cell phenotype and function. (
  • One marker (BrdU) tagged new cells, a second marker tagged neurons (nerve cells), and the third marker tagged glial cells (glia). (
  • However, they did find evidence of new glial cells in the neocortex. (
  • Macrophage interaction with mesangial cells through the action of leuckocyte adhesion molecules facilitates mesangial cell proliferation. (
  • This may be mediated by macrophage production of a number of mesangial cell growth factors or by modulating the mesangial matrix to promote mesangial cell growth. (
  • Mesangial cells may play an active role in this process, since mesangial cell production of chemoattractant molecules may be a key event in inducing macrophage recruitment into the injured mesangium. (
  • There the microbe encounters the alveolar macrophage (AMac) and submucosal dendritic cell (DC). (
  • However, there are some data showing that fibronectin has an inhibitory effect on HSC proliferation [7]. (
  • However, intestinal epithelial cell proliferation is not impeded, implying compensatory mechanisms. (
  • These results suggest that ERK5 provides a common bypass route in intestinal epithelial cells, which rescues cell proliferation upon abrogation of ERK1/2 signalling, with therapeutic implications in CRC. (
  • These ionocytes, known as NaR cells, are functionally similar to human intestinal epithelial cells. (
  • [ 2 ] Therefore, homeostatic proliferation can accelerate cell senescence, leading to the shortening of telomeres, the main function of which is to protect the chromosomes ends, helping to maintain the integrity of the genome. (