Basophils: Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Histamine Release: The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.Basophil Degranulation Test: An in vitro test used in the diagnosis of allergies including drug hypersensitivity. The allergen is added to the patient's white blood cells and the subsequent histamine release is measured.Immunoglobulin E: An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Receptors, IgE: Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).Cell Degranulation: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.Protein PrecursorsTryptases: A family of neutral serine proteases with TRYPSIN-like activity. Tryptases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.Anaphylaxis: An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.Hypersensitivity: Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Chymases: A family of neutral serine proteases with CHYMOTRYPSIN-like activity. Chymases are primarily found in the SECRETORY GRANULES of MAST CELLS and are released during mast cell degranulation.Antigens, CD63: Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Histamine: An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Immunity, Innate: The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.Specific Pathogen-Free Organisms: Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free.Immune System: The body's defense mechanism against foreign organisms or substances and deviant native cells. It includes the humoral immune response and the cell-mediated response and consists of a complex of interrelated cellular, molecular, and genetic components.Cilia: Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)Characidae: A family of fresh water fish in the order CHARACIFORMES, which includes the Tetras.Characiformes: An order of fresh water fish with 18 families and over 1600 species. The order includes CHARACINS, hatchetfish, piranhas, and TETRAS.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Gills: Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.Eichhornia: A plant genus of the family PONTEDERIACEAE that is used as a biological filter for treating wastewater.Myxobolus: The largest genus in the family Myxobolidae, class MYXOSPOREA, containing over 400 species.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Leukemia, Myelomonocytic, Acute: A pediatric acute myeloid leukemia involving both myeloid and monocytoid precursors. At least 20% of non-erythroid cells are of monocytic origin.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Physicians: Individuals licensed to practice medicine.Judgment: The process of discovering or asserting an objective or intrinsic relation between two objects or concepts; a faculty or power that enables a person to make judgments; the process of bringing to light and asserting the implicit meaning of a concept; a critical evaluation of a person or situation.Leukemia, Myelogenous, Chronic, BCR-ABL Positive: Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.Clinical Trials as Topic: Works about pre-planned studies of the safety, efficacy, or optimum dosage schedule (if appropriate) of one or more diagnostic, therapeutic, or prophylactic drugs, devices, or techniques selected according to predetermined criteria of eligibility and observed for predefined evidence of favorable and unfavorable effects. This concept includes clinical trials conducted both in the U.S. and in other countries.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Leukemia, Lymphoid: Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Oximetry: The determination of oxygen-hemoglobin saturation of blood either by withdrawing a sample and passing it through a classical photoelectric oximeter or by electrodes attached to some translucent part of the body like finger, earlobe, or skin fold. It includes non-invasive oxygen monitoring by pulse oximetry.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Hydrocephalus, Normal Pressure: A form of compensated hydrocephalus characterized clinically by a slowly progressive gait disorder (see GAIT DISORDERS, NEUROLOGIC), progressive intellectual decline, and URINARY INCONTINENCE. Spinal fluid pressure tends to be in the high normal range. This condition may result from processes which interfere with the absorption of CSF including SUBARACHNOID HEMORRHAGE, chronic MENINGITIS, and other conditions. (From Adams et al., Principles of Neurology, 6th ed, pp631-3)Oxyhemoglobins: A compound formed by the combination of hemoglobin and oxygen. It is a complex in which the oxygen is bound directly to the iron without causing a change from the ferrous to the ferric state.Umbilical Arteries: Specialized arterial vessels in the umbilical cord. They carry waste and deoxygenated blood from the FETUS to the mother via the PLACENTA. In humans, there are usually two umbilical arteries but sometimes one.Hemoglobins: The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.Spectrophotometry, Infrared: Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Atlases as Topic: Collections of illustrative plates, charts, etc., usually with explanatory captions.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cervical Atlas: The first cervical vertebra.Interleukin-6: A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.

*Mast cell

Mast cells are very similar to basophil granulocytes (a class of white blood cells) in blood. Both are granulated cells that ... and CD34+ mast cell precursors. Mutations in c-Kit are associated with mastocytosis. Mastocytomas, or mast cell tumors, can ... Other neoplastic disorders associated with mast cells include mast cell sarcoma and mast cell leukemia. Mast cell activation ... connective tissue-type mast cells and mucosal mast cells. The activities of the latter are dependent on T-cells. Mast cells are ...

*Clonal hypereosinophilia

Hematopoietic stem cells give rise to: 1) myeloid precursor cells that differentiate into red blood cells, mast cells, blood ... B-cell lymphoma, T-cell lymphomas, T cell leukemias, and Langerhans cell histiocytosis. Other hematological diseases are ... basophils, monocytes, and eosinophils; or 2) lymphoid precursor cells which differentiate into T lymphocytes, B lymphocytes, or ... Such mutations occur in hematological stem cells and/or their daughter myeloid precursor and lymphoid precursor cells; commonly ...

*Cysteinyl leukotriene receptor 1

... mast cells, dendritic cells, and B-lymphocytes. ALOX5 metabolizes arachidonic acid to the 5,6-epoxide precursor, LTA4, which is ... and mucin secretion by goblet cells, goblet cell metaplasia, and epithelial cell hypertrophy in the membranes of the ... CYSLTR1 mRNA is expressed in lung smooth muscle, lung macrophages, monocytes, eosinophils, basophils, neutrophils, platelets, T ... mast cells, pancreas, small intestine, prostate, interstitial cells of the nasal mucosa, airway smooth muscle cells, bronchial ...

*SIGLEC8

In mast cells generated from CD34+ precursors, Siglec-8 expression peaks at 4 weeks of differentiation, in parallel with FcεRIα ... mast cell, and basophil siglec-8 expression". Journal of Clinical Immunology. 31 (6): 1045-53. doi:10.1007/s10875-011-9589-4. ... Siglec-8 is expressed by human eosinophils, mast cells, and, to a lesser extent, basophils. It has thus garnered attention as a ... Only low levels are detected on the human mast cell sub-line HMC-1.1; however, the HMC-1.2 cell line, which bears a second KIT ...

*CCL8

MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are ... and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell ... The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 ... 2010). "CCL8/MCP-2 is a target for mir-146a in HIV-1-infected human microglial cells". FASEB J. 24 (7): 2292-300. doi:10.1096/ ...

*List of distinct cell types in the adult human body

Basophil granulocyte Hybridoma cell Mast cell Helper T cell Suppressor T cell Cytotoxic T cell Natural killer T cell B cell ... cells Spindle neuron Pyramidal cells Place cells Grid cells Speed cells Head direction cells Betz cells Stellate cells Boundary ... Myoepithelial cell of iris Myoepithelial cell of exocrine glands Erythrocyte (red blood cell) Megakaryocyte (platelet precursor ... Basket cells Cartwheel cells Stellate cells Golgi cells Granule cells Lugaro cells Unipolar brush cells Martinotti cells ...

*Granulocyte

... dendritic cell, B cell, mast cell, neutrophil, and basophil functions), they are involved in the destruction of tumor cells, ... Neutrophils do not normally exit the bone marrow until maturity, but during an infection neutrophil precursors called ... Basophils are one of the least abundant cells in bone marrow and blood (occurring at less than two percent of all cells). Like ... There are four types of granulocytes: Basophils Eosinophils Neutrophils Mast cells Their names are derived from their staining ...

*Phospholipase A2

PLA2 action can release histamine from rat peritoneal mast cells. It also causes histamine release in human basophils. Due to ... Argiolas A, Pisano JJ (Nov 1983). "Facilitation of phospholipase A2 activity by mastoparans, a new class of mast cell ... Arachidonic acid is both a signaling molecule and the precursor for the synthesis of other signaling molecules termed ... "Pharmacological study of phospholipase A2-induced histamine release from rat peritoneal mast cells". J Pharmacobiodyn. 1989 Sep ...

*Eosinophil

... s, along with basophils and mast cells, are important mediators of allergic responses and asthma pathogenesis and are ... Zheutlin L, Ackerman S, Gleich G, Thomas L (1984). "Stimulation of basophil and rat mast cell histamine release by eosinophil ... as they differentiate from myeloid precursor cells. Their lineage fate is determined by transcription factors, including GATA ... can inhibit proliferation of T cells, suppress antibody production by B cells, induce degranulation by mast cells, and ...

*Outline of immunology

CFU-Mast Mast cell precursors B cells Antigen receptor - B cell receptor (BCR) Subunits- Immunoglobulin heavy chain / ... Dendritic cell / Langerhans cell precursor Promonocyte CFU-Baso (Basophil precursor) CFU-Eos (Eosinophil precursor) ... Commonly termed CD4+ T cells Th1 cells Th2 cells Th3 cells Th17 cells TFH cells - Follicular helper T cells Cytotoxic T cells ... Lymphoid cells B cells Plasma B cells Memory B cells B-1 cells B-2 cells (the conventional B cells most texts refer to) ...

*IL1A

... mast cells and basophils, endothelial cells, platelets, monocytes and myeloid cell lines, blood T-lymphocytes and B-lymphocytes ... A wide variety of other cells only upon stimulation can be induced to transcribe the IL-1α genes and produce the precursor form ... astrocytes, kidney mesangial cells, Langerhans cells, dermal dendritic cells, natural killer cells, large granular lymphocytes ... microglia, blood neutrophils, lymph node cells, maternal placental cells and several other cell types. These data suggest that ...

*Granzyme B

... found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The ... Basophils secrete granzyme B to degrade endothelial cell-cell contacts allowing extravasation to sites of inflammation. ... Granzyme B is thought to have evolved from a granzyme H related precursor and is more effective at lower concentrations than ... Granzyme B is secreted by regulatory T cells to kill CD4+ T cells that have not been exposed to host cells that are restricted ...

*Interleukin-1 family

... mast cells, basophils, eosinophils, invariant natural killer and natural killer T cells. It is involved in allergic and ... The synthesis of IL-1β precursor (and IL-18) is induced by stimulation of innate immune cells by Toll-like receptors (TLRs) or ... because it acts as DAMPs after its release to extracellular space of cells in the context of immunologic not-silent cell death ... IL-1α is synthesized as a precursor protein and it is constitutively stored in the cytoplasm of cells with mesenchymal origin ...

*CCL2

Basophils and mast cells that are treated with CCL2 release their granules to the intercellular space. This effect can be also ... CCR2 and CCR4 are two cell surface receptors that bind CCL2. CCL2 exhibits a chemotactic activity for monocytes and basophils. ... The CCL2 protein precursor contains a signal peptide of 23 amino acids. In turn, the mature CCL2 is 76 amino acids long. The ... CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or ...

*Major basic protein

The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, and activates ... 1992). "Purification and cDNA cloning of a novel factor produced by a human T-cell hybridoma: sequence homology with animal ... Barker RL, Gleich GJ, Pease LR (1988). "Acidic precursor revealed in human eosinophil granule major basic protein cDNA". J. Exp ... Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), also known as PRG2, is a ...

*Innate immune system

The innate leukocytes include: Natural killer cells, mast cells, eosinophils, basophils; and the phagocytic cells include ... Mast cells are a type of innate immune cell that reside in connective tissue and in the mucous membranes. They are intimately ... Nevertheless, invertebrates possess mechanisms that appear to be precursors of these aspects of vertebrate immunity. Pattern ... Rather, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells, recognizing such cells by a ...

*CFU-GEMM

... and the myeloid precursor (CFU-GEMM). The CFU-GEMM cell is capable of differentiating into white blood cells, red blood cells, ... mast cell or myeloblast based on the presence of specific factors that encourage the cell to choose a lineage to follow. The ... G-CSF, M-CSF, IL-5, IL-4, and IL-3 stimulate the production of neutrophils, monocytes, eosinophils, basophils, and platelets, ... CFU-GEMM cells are the multipotential progenitor cells for myeloid cells; they are thus also called common myeloid progenitor ...

*Immune system

... innate lymphoid cells, mast cells, eosinophils, basophils, and natural killer cells. These cells identify and eliminate ... Gamma delta T cells (γδ T cells) possess an alternative T-cell receptor (TCR) as opposed to CD4+ and CD8+ (αβ) T cells and ... Many species, however, utilize mechanisms that appear to be precursors of these aspects of vertebrate immunity. Immune systems ... Rather, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells, recognizing such cells by a ...

*Chemorepulsion

Other innate leukocytes include natural killer cells, mast cells, eosinophils, basophils, macrophages, and dendritic cells. ... Different cell types to which the term "immunorepulsion" could potentially be applied include: Myeloid lineage cells (monocytes ... 2001). "Stromal-derived factor-1 in human tumors recruits and alters the function of plasmacytoid precursor dendritic cells". ... dendritic cells) and Lymphoid lineage cells (T-cells, B-cells, NK-cells). The chemorepulsion of immune cells was first ...

*Inflammation

"Mast cell proteases as pharmacological targets". European Journal of Pharmacology. Pharmacological modulation of Mast cells and ... are a necessary precursor to muscle growth. As a response to muscular contractions, the acute inflammatory response initiates ... Basophils. 778: 44-55. doi:10.1016/j.ejphar.2015.04.045. PMC 4636979 . PMID 25958181. Libby, P (Dec 19-26, 2002). "Inflammation ... dendritic cells, histiocytes, Kupffer cells and mast cells. These cells possess surface receptors known as pattern recognition ...

*GATA1

... the ability of erythroid precursor cells to proliferate and proteins that control the capacity of the erythroid precursor cell ... GATA1 is required for the maturation of red blood cells, megakaryocytes, mast cells and eosinophils. GATA1 mutant mice die in ... "Expression of mRNA for the GATA-binding proteins in human eosinophils and basophils: potential role in gene transcription". ... Fujiwara Y, Browne CP, Cunniff K, Goff SC, Orkin SH (Oct 1996). "Arrested development of embryonic red cell precursors in mouse ...

*Antibody

... lysis of cells (binding to complement), and degranulation of mast cells, basophils, and eosinophils (binding to FcεR). In ... LeBien TW (1 July 2000). "Fates of human B-cell precursors". Blood. 96 (1): 9-23. PMID 10891425. Archived from the original on ... The antibody isotype of a B cell changes during cell development and activation. Immature B cells, which have never been ... B cell activation follows engagement of the cell-bound antibody molecule with an antigen, causing the cell to divide and ...

*Index of HIV/AIDS-related articles

... mast cell - MedlinePlus - mega-HAART - memory T cells - meninges - meningitis - messenger RNA - metabolism - metastasis - MHC ... B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - basophil - bDNA test ... precursor cells - prevalence - primary HIV infection - primary isolate - primaquine - proctitis - prodrome - prodrug - ... T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 cells - Tanner staging - TAT - TB - template - TeachAIDS - ...

*Glossary of biology

mast cell A cell filled with basophil granules, found in numbers in connective tissue and releasing histamine and other ... effector cell Plasma cells, also called plasma B cells, plasmocytes, plasmacytes, or effector B cells, are white blood cells ... the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other ... cell nucleus The "control room" for the cell. The nucleus gives out all the orders. cell plate Grown in the cell's center, it ...

*International Classification of Diseases for Oncology

NOS or Extracutaneous mastocytoma Mast cell tumor, NOS M9740/3 Mast cell sarcoma Malignant mast cell tumor Malignant ... Basophil adenocarcinoma Mucoid cell adenocarcinoma M8310/0 Clear cell adenoma M8310/3 Clear cell adenocarcinoma, NOS Clear cell ... NOS M9728/3 Precursor B-cell lymphoblastic Lymphoma (see also M9836/3) M9729/3 Precursor T-cell lymphoblastic Lymphoma (see ... NOS M8001/3 Tumor cells, malignant M8002/3 Malignant tumor, small cell type M8003/3 Malignant tumor, giant cell type M8004/3 ...
RESUMEN. The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes.. Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes in ,0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils.. In addition to clinical history, skin prick test, and specific IgE determination, BAT can be a part of the diagnostic evaluation of patients with food-, insect venom-, and drug allergy and chronic urticaria. It may be helpful in determining the clinically relevant allergen.. Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is ...
TY - JOUR. T1 - Proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. Dominant expression of the quiescent state in heterokaryons. AU - Laeng, H.. AU - Harris, David T.. AU - Schindler, R.. PY - 1985. Y1 - 1985. N2 - Normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-SB-4) of the P-815 murine mastocytoma. Upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-SB-4 cells reentered S phase of the cell cycle, while mast cell × 41-SB-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. The ...
Background: Mast cells infiltrate the bronchial smooth muscle (BSM) in asthmatic patients, but the mechanism of mast cell adhesion is still unknown. The adhesion molecules CD44 (i.e. hyaluronate receptor) and CD51 (i.e. vitronectin receptor) are widely expressed and bind to many extracellular matrix (ECM) proteins. The aims of the study are (i) to identify the role of ECM in mast cell adhesion to BSM and (ii) to examine the role of CD51 and CD44 in this adhesion.. Methods: Human lung mast cells, human mast cell line (HMC-1), and BSM cells from control donors or asthmatic patients were cultured in the presence/absence of various cytokines. Mast cell-BSM interaction was assessed using 3H-thymidine-pulsed mast cells, confocal ...
Treatment of allergic disease by decreasing circulating IgE with anti-IgE Abs is currently under clinical study. Based on previous unrelated studies, it appeared likely that Fc(epsilon)RI expression on basophils and mast cells might also be regulated by levels of circulating IgE Ab. Therefore, the expression of IgE and Fc(epsilon)RI on human basophils was examined in 15 subjects receiving humanized anti-IgE mAb intravenously. Treatment with the anti-IgE mAb decreased free IgE levels to 1% of pretreatment levels and also resulted in a marked down-regulation of Fc(epsilon)RI on basophils. Median pretreatment densities of Fc(epsilon)RI were approximately 220,000 receptors per basophil and after 3 mo of treatment, the densities had decreased to a median of 8,300 receptors per basophil. Flow cytometric studies, conducted in parallel, showed similar results and also showed in a ...
Interactions between products of the mouse W locus, which encodes the c-kit tyrosine kinase receptor, and the Sl locus, which encodes a ligand for c-kit receptor, which we have designated stem cell factor (SCF), have a critical role in the development of mast cells. Mice homozygous for mutations at either locus exhibit several phenotypic abnormalities including a virtual absence of mast cells. Moreover, the c-kit ligand SCF can induce the proliferation and maturation of normal mast cells in vitro or in vivo, and also can result in repair of the mast cell deficiency of Sl/Sld mice in vivo. We now report that administration of SCF intradermally in vivo results in dermal mast cell activation and a mast cell-dependent acute inflammatory response. This effect is c-kit receptor dependent, in that ...
c-kit ligand (KL) activated mouse bone marrow-derived mast cells (BMMC) for the dose- and time-dependent release of arachidonic acid from cell membrane phospholipids, with generation of leukotriene (LT) C4 in preference to prostaglandin (PG)D2. KL at concentrations of 10 ng/ml elicited half-maximal eicosanoid generation and at concentrations of , 50 ng/ml elicited a maximal generation of approximately 15 ng LTC4 and 1 ng PGD2 per 10(6) cells, with 20% net beta-hexosaminidase release 10 min after stimulation. Of the other cytokines tested, none, either alone or in combination with KL, elicited or modulated the immediate phase of mediator release by BMMC, indicating strict specificity for KL. Activation of BMMC in response to KL was accompanied by transient phosphorylation of cytosolic phospholipase A2 and reversible translocation of 5-lipoxygenase to a cell membrane fraction 2-5 min after stimulation, when the rate of ...
Resveratrol, a polyphenol abundant in peanuts, red wine and the skin of grapes, has been shown to have anti-cancer, anti-oxidant and anti-inflammatory activities, and may also have beneficial effects on allergic inflammation. We investigated the effects of resveratrol on human mast cell activation in comparison to the anti-allergy drug tranilast. In LAD2 mast cells, both resveratrol and tranilast inhibited degranulation induced by the mast cell activators substance P, IgE/anti-IgE, and compound 48/80. Resveratrol inhibition was immediate, preventing degranulation when added simultaneously to physiological stimuli, and the effect was sustained for up to 24 hrs. The inhibitory effect was not cAMP dependent, but may be attributable to calcium modulation, as resveratrol, and to a lesser extent tranilast, prevented substance P-induced increases in intracellular calcium. Resveratrol attenuated ...
TY - JOUR. T1 - Leflunomide inhibits PDK1/Akt pathway and induces apoptosis of human mast cells. AU - Sawamukai, Norifumi. AU - Saito, Kazuyoshi. AU - Yamaoka, Kunihiro. AU - Nakayamada, Shingo. AU - Ra, Chisei. AU - Tanaka, Yoshiya. PY - 2007/11/15. Y1 - 2007/11/15. N2 - Mast cells release many inflammatory mediators that play an important role not only in allergic diseases but also in chronic inflammatory diseases, autoimmune diseases, and others. A lot of mast cells exist in synovium of rheumatoid arthritis, and it is known that synovitis does not occur in mast cell-deficient mice. Thus, it is thought that mast cells play a very important role in rheumatoid arthritis pathogenesis. Leflunomide is a drug used clinically in the treatment of rheumatoid arthritis. We used clinical doses of ...
Exposure to monomeric IgE in vitro markedly upregulated the ability of mature mouse peritoneal mast cells or mouse BMCMCs or cloned mast cells to bind IgE. Two separate lines of evidence indicate that this response largely, if not entirely, reflected the increased surface expression of FcεRI. First, while mouse mast cells also express FcγRII/ III ((17), (18)), which can bind IgE immune complexes ((18)), virtually all of the binding of monomeric IgE to mouse mast cells that is detectable under the conditions used in our experiments reflects binding of the ligand to a single class of high affinity binding sites, i.e., FcεRI ((18)). Second, we used anti-IgE to immunoprecipitate surface-bound IgE, and associated IgE receptors, from lysates of BMCMCs that had been first incubated with or without IgE at 5 μg/ml for 21 h and then exposed briefly to excess IgE just before recovery ...
TY - JOUR. T1 - Progress in allergy signal research on mast cells. T2 - Signal regulation of multiple mast cell responses through FcεRI. AU - Yamasaki, Shou. AU - Saito, Takashi. PY - 2008/4/8. Y1 - 2008/4/8. N2 - The crosslinking of FcεRI by IgE and antigen (Ag) on mast cells initiates activation cascades that lead to allergic responses. Although it was thought that IgE binding to FcεRI is a passive "sensitization", recent reports suggest that IgE actively promotes mast cell survival in the absence of Ag. However, it is largely unknown how these distinct responses are delivered through the same receptor, FcεRI, depending on the types of stimli. As an underlying molecular mechanism for the generation of diverse responses through FcεRI, we found that the quantity and the duration of the signal through the FcεRI γ chain (FcRγ) determine different mast ...
In this study the diversity of mast cell proteases and some of the factors regulating mast cell growth and protease expression were examined in rodents. Five proteases were isolated from mouse small intestinal mucosa and their substrate specificities defined. The isolated proteases were all of mast cell origin and were chymotrypsin-like in their substrate specificities. The proteases were all identified as variants of mouse mast cell protease-1 which differed only in their carbohydrate moieties. Despite the fact that these enzymes shared a common core polypeptide they all differed significantly in the rate at which they hydrolysed synthetic substrates and in the rates at which they were inhibited by α1-proteinase inhibitor. A related, but distinct protease was isolated from peritoneal cavity mast cells of mice. This enzyme, also a chymase, ...
Mast cells are best known for their function in hypersensitive reactions, where aggregation of FcRI leads to the release of mast cell mediators leading to hypersensitive symptoms. although activation-induced success is certainly suffered, suggesting a minimal function for Bcl-XL, Bcl-2, Mcl-1 and Bcl-w. Reducing but not really amounts by siRNA inhibited activation-induced mast cell success. We also demonstrate that mast cell phrase of Bfl-1 is certainly raised in birch-pollen-provocated epidermis and in lesions of atopic dermatitis and psoriasis sufferers. Used jointly, our outcomes high light Bfl-1 as a main effector in activation-induced individual mast cell success. Launch Mast cells are known to end up being central regulators and effectors in allergic illnesses. When a multivalent antigen binds to IgE ...
By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium ...
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
The inhibitory myeloid immunoglobulin receptor CD300a (IRp60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to Th2-type immunity and allergy. Basophils homogeneously expressed CD300a as well as the inhibitory receptor CD200R on their cell surface, and these expressions increased after anti-IgE stimulation. IgE-mediated basophil degranulation was also significantly inhibited by crosslinking of either CD200R or CD300a (by 90% and 50%, respectively). Inhibitory SHIP-1 phosphorylations were also induced by CD200R and CD300a, although they were not noticeably increased by IgE-dependent activation. We conclude ...
Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled with histadine, proteolytic enzymes, leukotrienes, and proteoglycans. Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can be identified by expression of CD200R3 and FceRIa. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these cell types using standard staining techniques.) *Produced only in pregnancy by the developing embryo. B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology: CD-ROM and Guide. Oxford University Press. ISBN 978-0195151732. MedicalMnemonics.com: ...
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit ...
Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat basophilic leukaemia cell line RBL-2H3, and compared their properties with each other and with plasma-membrane vesicles. DRM fractions were isolated as sealed unilamellar vesicles of similar size (135-170 nm diameter), using either sucrose-density-gradient sedimentation or gel-filtration chromatography. Lipid rafts isolated using Brij-96 and Triton X-100 differed in density, protein ...
Aims The essential role of basophils as an initiator of chronic allergic reaction has been elucidated in mouse models. The aim of this present study was to analyse the in situ immunolocalisation of basophils and other relevant inflammatory cells in chronic allergic keratoconjunctivitis.. Methods Transmission electron microscopic (TEM) analysis was carried out to examine the existence of basophils in the giant papillae obtained from atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) patients. Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-FcɛRI-β antibodies.. Results TEM analysis confirmed the existence of basophils in the giant papillae. Small clusters of basophils were ...
View details of top basophil count hospitals in Thane. Get guidance from medical experts to select best basophil count hospital in Thane
Looking for online definition of basophil substance in the Medical Dictionary? basophil substance explanation free. What is basophil substance? Meaning of basophil substance medical term. What does basophil substance mean?
Looking for online definition of basophilia in the Medical Dictionary? basophilia explanation free. What is basophilia? Meaning of basophilia medical term. What does basophilia mean?
Resistance to infestation by larval Amblyomma americanum or Rhipicephalus sanguineus ticks was transferred to naive guinea pigs with peritoneal exudate cells (PEC) or serum from donors immunized by prior infestation with homologous tick larvae. In the A. americanum system, PEC transfer induced 87% tick rejection, which was similar to the level of resistance in actively sensitized hosts. In the R. sanguineus system, PEC conferred resistance (39% rejection) that was weaker than in actively sensitized hosts (57% rejection). In both systems, immune serum conferred significant but weaker resistance (20 to 29% rejection). In actively sensitized hosts, resistance to each tick species was specific, but there was considerable cross-reactive resistance. Basophils dominated the 24-hr challenge feeding sites of A. americanum ticks in actively sensitized hosts (69% of the infiltrate) and recipients of sensitized PEC (69%). Mononuclear cells were dominant ...
TY - JOUR. T1 - A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells. AU - Barth, Erin E.. AU - Hallworth, Richard J.. AU - Nichols, Michael G.. PY - 2005/5. Y1 - 2005/5. N2 - Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint will necessarily depend on the characteristics of the damage induced by the agent. By using multiple biological probes, it is possible to get a more complete description of the type of damage induced. Photodamage was induced in rat basophilic leukemia cells by either 254-nm UVC light exposure or rose bengal photosensitization. Damage was measured by three quantitative assays employing fluorescent probes: calcein, to measure nonspecific esterase activity, propidium iodide (PI), to measure loss of plasma ...
Allergic symptoms are caused by an initial systemic histamine release by activated basophiles and mast cells, that may lead to shock with laryngeal edema, lower-airway obstruction and hypotension. This is why basophiles are considered with mast cells to be the key cells in allergic diseases. Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has only been found in mammals. It plays an important role in allergy, and is especially associated with type 1 hypersensitivity. There are receptors (FcεR) for the constant region of IgE, the Fc region, on several types of cells, including Mast cells and Basophils. Basophils contain many granules inside the cell, which are filled with a variety of active substance triggering an allergic response upon degranulation. The ...
TY - JOUR. T1 - Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test. AU - Okamoto, Kaoru. AU - Kagami, Michiko. AU - Kawai, Manabu. AU - Mori, Yuji. AU - Yamawaki, Kazuo. AU - Nakajima, Yoichi. AU - Kondo, Yasuto. AU - Tsuge, Ikuya. PY - 2019/1. Y1 - 2019/1. UR - http://www.scopus.com/inward/record.url?scp=85058961080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85058961080&partnerID=8YFLogxK. U2 - 10.1016/j.alit.2018.08.001. DO - 10.1016/j.alit.2018.08.001. M3 - Letter. C2 - 30190098. AN - SCOPUS:85058961080. VL - 68. SP - 130. EP - 131. JO - Allergology International. JF - Allergology International. SN - 1323-8930. IS - 1. ER - ...
Immediate selective hypersensitivity reactions to pyrazolones are thought to be mediated by specific-IgE antibodies. The diagnosis of selective reactions to pyrazolones is mainly based on the clinical history, skin testing and/or drug provocation test (DPT). Skin testing has low sensitivity and has potential risk of eliciting an anaphylactic response as well as DPT. Therefore the use of in vitro tests could be a safe and straightforward approach, although till now no well-validated test is available. Basophil activation test (BAT) has been proposed as a new in vitro diagnostic tool for different drug reactions although its sensibility is not optimal. The aim of this study was to analyze the potential usefulness of dipyrone metabolites to improve BAT sensitivity. ...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
Basophil (好塩基球 Kōenkikyū) is a minor character in Cells at Work!. He is a type of white blood cell, specifically Basophil. He is capable of triggering the release of histamines when encountering an antigen. He also can allure eosinophils and neutrophils when they are needed. His role is not...
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
A nice review about the early non-T-cell source of IL-4 from basophils that act as Th2-inducing antigen-presenting cells. Even though we have shown that during LN responses to alum-precipitated antigens IL-4 is dispensable for the early induction of Th2-features including B cell maturation and helper T cell differentiation more, it is important to keep in mind that the basophil-source of IL-4 may have implication during the clearance of helminth infections and the development/maintenance/ augmentation of allergic diseases by induction of IgE production, activation of mast cells and basophils, as well as eosinophilic inflammation. An important role for basophils is unquestionable for they have been shown (together with mast cells) to release large amounts of IL-4 and IL-13 ...
Most histamine in the body is generated in granules in mast cells and in white blood cells called basophils andeosinophils. Mast cells are especially numerous at sites of potential injury -- the nose, mouth, and feet, internal body surfaces, and blood vessels. Non-mast cell histamine is found in several tissues, including the brain, where it functions as a neurotransmitter. Another important site of histamine storage and release is the enterochromaffin-like (ECL) cell of the stomach.. The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic. These cells, if sensitized byIgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen. Certain amines and alkaloids, including such drugs as morphine, and curare ...
Hemocytes of Mytilus edulis were examined cytologically and cytochemically. On the basis of structure, staining reactions, and phagocytic behavior, they were divided into two main groups: basophilic hemocytes and eosinophilic granular hemocytes (granulocytes). The basophilic cells were further divided into small lymphocytes and larger phagocytic macrophages reactive for lysosomal hydrolases. Mitosis was observed in granulocytes and in small lymphoid cells, believed to be the stem cells for the basophilic cell line. A few cells appeared to be intermediate between lymphocytes and small granulocytes. Macrophages were the main cell type involved in the clearance of injected carbon particles. However, granulocytes did show some phagocytic activity. Brown cells displaying apparent amoebocytic behavior were found to contain Fe3+ and Pb2+ in cytoplasmic inclusions, some of which were also reactive ...
This study shows that histamine is liberated from human basophils by betaxolol and metipranolol and, to a significantly lesser extent, by timolol. The fact that we detected LDH in the supernatant indicated that the β blocker concentrations we used were cytotoxic to leucocytes. This cytotoxic effect may also explain the histamine liberation because we do not consider that high concentrations of β blockers are less toxic to basophils than to other leucocytes. Also, in previous studies other authors have used LDH levels to determine the cytotoxic effects on basophils in similar test conditions.14 Betaxolol and timolol formulations are commercially available in 0.1%, 0.25%, and 0.5% concentrations and metipranolol formulations are commercially available in 0.1%, 0.3%, and 0.6% which equals approximately 2.5 × 10-3 to 2 × 10-2 M. The cytotoxicity found in these experiments therefore occurs at concentrations that ...
U.S., June 15 -- ClinicalTrials.gov registry received information related to the study (NCT03182491) titled Mechanisms of Anaphylaxis on June 7. Brief Summary: The purpose of this study is to explore different mechanisms for anaphylaxis and find novel biomarkers for this hypersensitivity syndrome. The study participants are patients with anaphylaxis, patients with mild allergic reactions, and patients with febrile transfusion reactions. We will also include a group of healthy controls. Study Start Date: Study Type: Observational [Patient Registry] Condition: Anaphylaxis Allergy Transfusion Reaction Febrile Transfusion Reaction Intervention: Diagnostic Test: Biomarkers (platelet activating factor [PAF], anaphylatoxins) and basophil activation test (BAT) Analysis of biomarkers and basophil activation test Recruitment Status: Recruiting Sponsor: Haukeland University Hospital Information provided by (Responsible Party): Haukeland University Hospital Published by HT Digital Content Services with ...
Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, ...
Allergic diseases represent a significant burden in industrialized countries, but why and how the immune system responds to allergens remain largely unknown. Because many clinically significant allergens have proteolytic activity, and many helminths express proteases that are necessary for their life cycles, host mechanisms likely have evolved to detect the proteolytic activity of helminth proteases, which may be incidentally activated by protease allergens. A cysteine protease, papain, is a prototypic protease allergen that can directly activate basophils and mast cells, leading to the production of cytokines, including IL-4, characteristic of the type 2 immune response. The mechanism of papains immunogenic activity remains unknown. Here we have characterized the cellular response activated by papain in basophils. We find that papain-induced IL-4 production requires calcium flux and ...
The crucial symptom of atopic eczema is itch. Acupuncture has been shown to exhibit a significant effect on experimental itch; however, studies focusing on clinical itch in atopic eczema and corresponding mechanisms are lacking. The study design was a unicenter, single-blinded (observer), prospective, randomized clinical pilot trial with an additional experimental part. In 10 patients with atopic eczema, we investigated the effect of acupuncture treatment (n = 5) compared to no treatment (n = 5) on itch intensity and in vitro basophil CD63 expression upon allergen stimulation (house dust mite and timothy grass pollen) in a pilot trial. Results: Mean itch intensity in a visual analog scale was rated significantly lower in the acupuncture group (-25% ± 26% [day 15-day 0]; -24% ± 31% [day 33-day 0]) than in the control group (15% ± 6% [day 15-day 0]; 29% ± 9% [day 33-day 0]). From day 0 (before treatment) to day 15 (after 5 acupuncture treatments) as well as day 33 (after 10 acupuncture ...
Food allergy is defined as an adverse immunological response to food. Some of the tests discussed in this chapter not only aid in the diagnosis of food allergies, but also are useful in monitoring the natural history of patients' food allergies over time, from diagnosis to oral tolerance. Once food allergy has been identified as the likely cause of symptoms, confirmation of the diagnosis and identification of the implicated food(s) can begin. There are a number of tools that aid in the diagnosis of food allergy, some of which are more commonly used, and they vary in their ability to provide an accurate diagnosis. Available studies include in vivo tests such as skin prick and intradermal testing, oral food challenges (OFCs), elimination diets, and patch testing and in vitro tests such as quantification of food-specific IgE and basophil histamine release (BHR). Tests that have not proven helpful in food allergy diagnosis include quantification of food-specific IgG, total serum IgE levels, ...
U.S. Environmental Protection Agency (EPA) provide evaluations and is despostory of cataloged analysis. They also rank problems like, like indoor air pollution. commonly called sick building syndrome. They state that it is costing businesses $60 billion annually. Ill will get into the data later but I want to discuss, how do we protect human health and quality of life? By reducing chemical exposure and improve indoor air quality?. What Im finding out its not just the enviroment, its a humans immune system. Im not trying to be clever but create a debate that simply states that IAQ is a moving target. Molds that are everywhere, I have to refere to an expert, Serum IgE "Specific to Indoor Molds, Measured by Basophil Histamine Release, is Associated with Building-related Symptoms in Damp Buildings" FROM: Inflamm Res 2001; 50 (4) Apr: 227-31 Lander F, Meyer HW, Norn S National Work Environment Authority, Copenhagen, Denmark. [email protected] dk OBJECTIVE: To study the relationship between ...
The release mechanism of chemical mediators from basophils and mast cells was discussed when these cells were stimulated by different antigens and anti-IgE. 1. Ca(2+) influx into mast cells increased after stimulation by antigen. The increased Ca(2+) uptake by mast cells was inhibited by antiallergic agents, disodium cromoglycate (DSCG) and tranilast, and calcium antagonists, nifedipine and nicardipine. 2. The dose-response curve of histamine release by antigen was different from that by anti-IgE. The maximum release of histamine by house dust was, however, similar to that by anti-IgE. House dust- and anti-IgE-induced release of histamine increased with higher- serum IgE levels. On the contrary, the release of histamine by Candida albicans was not releated to serum IgE levels, and the release by C. albicans did not parallel therelease by anti-IgE ...
Looking for Charcot-Leyden crystal? Find out information about Charcot-Leyden crystal. a solid body bounded by natural plane faces that are the external expression of a regular internal arrangement of constituent atoms, molecules, or ions. Explanation of Charcot-Leyden crystal
OMALIZUMAB TRIAL. The results of the first of three phase III clinical trials of the effects of omalizumab in patients with chronic idiopathic urticaria were reported online in the New England Journal of Medicine this week.. Urticaria (hives) occurs when mast cells in the skin are triggered to release histamine and other inflammatory mediators stored in cytoplasmic granules (degranulation). In acute urticaria due to allergic reactions, degranulation is triggered when allergen is bound by IgE antibodies attached to FcεRI receptors on the mast cell surface. However, chronic urticaria is not IgE mediated and the mechanism by which mast cells are triggered to degranulate and release histamine is not known.. Omalizumab is a recombinant humanized monoclonal antibody which binds rapidly to circulating IgE, inhibiting its attachment to FcεRI receptors on basophils and ...
Ticks, blood-sucking arthropods, serve as vectors for transmission of infectious diseases including Lyme borreliosis. After tick infestation, several animal species develop resistance to subsequent infestations, reducing the risk of transmission. In a mouse model, basophils reportedly infiltrate tick-feeding sites during the 2nd but not 1st infestation and play a crucial role in the manifestation of acquired tick resistance. However, the mechanism underlying basophil recruitment to the 2nd tick-feeding site remains ill-defined. Here we investigated cells and their products responsible for the basophil recruitment. Little or no basophil infiltration was detected in T cell-deficient mice, and adoptive transfer of CD4+ but not CD8+ T cells reconstituted it. Il3 gene expression was highly upregulated at the 2nd tick-feeding site, and adoptive transfer of IL-3-sufficient but not -deficient CD4+ T cells conferred ...
TY - JOUR. T1 - Effect of loratadine on histamine release induced by antigen-antibody reaction. AU - Kamei, Chiaki. AU - Sugimoto, Yukio. AU - Yamaji, Masako. AU - Takada, Miho. PY - 1996. Y1 - 1996. N2 - Loratadine caused an inhibition of histamine release from rat peritoneal mast culls induced by passively sensitized mast cells, and IC 50 was 9.57 μM. SCH 34117, a metabolite of loratadine, also inhibited histamine release from mast cells, and its potency was more than that of loratadine. Moreover, in ex vivo experiments, loratadine (5 mg/kg, p.o.) as well as terfenadine provided a relatively potent inhibitory effect on histamine release from lung pieces of actively sensitized guinea pigs exposed to antigen.. AB - Loratadine caused an inhibition of histamine release from rat peritoneal mast culls induced by passively sensitized mast cells, and IC 50 was 9.57 μM. SCH 34117, ...
Intracellular expression of Ab fragments has been efficiently used to inactivate therapeutic targets, oncogene products, and to induce viral resistance in plants. Ab fragments expressed in the appropriate cell compartment may also help to elucidate the functions of a protein of interest. We report in this study the successful targeting of the protein tyrosine kinase Syk in the RBL-2H3 rat basophilic leukemia cell line. We isolated from a phage display library human single-chain variable fragments (scFv) directed against the portion of Syk containing the Src homology 2 domains and the linker region that separates them. Among them, two scFv named G4G11 and G4E4 exhibited the best binding to Syk in vivo in a yeast two-hybrid selection system. Stable transfectants of RBL-2H3 cells expressing cytosolic G4G11 and G4E4 were established. Immunoprecipitation experiments showed that intracellular G4G11 and G4E4 bind to Syk, but do not ...
BACKGROUND IgE sensitization to hazelnut is common, especially in birch endemic areas. However, its clinical significance often needs to be confirmed by a food challenge. OBJECTIVE To evaluate the clinical significance of IgE antibodies to hazelnut components and basophil allergen threshold sensitivity (CD-sens) to hazelnut, in relation to double-blind placebo-controlled food challenge (DBPCFC) in children with a suspected hazelnut allergy. METHODS Forty children underwent a DBPCFC. CD-sens to hazelnut as well as IgE antibodies to hazelnut and its components Cor a 1, Cor a 8, Cor a 9 and Cor a 14 were analysed. Serum tryptase was measured before, during and after DBPCFC. RESULTS Eight children had a positive DBPCFC, and all of them had a high CD-sens value to hazelnut. Of the 32 children that passed the DBPCFC, 31 were very low or negative in CD-sens. A positive DBPCFC corresponded with significantly higher CD-sens values (median 8.9, range 3.3-281) compared to children negative in challenge
INDICATIONS. Speman promotes spermatogenesis by improving the testicular, seminal vesicle and epididymal functions. Speman improves the sperm count and the quality of semen by increasing the LH-FSH producing basophil cells in the pituitary.. INSTRUCTIONS Please consult your physician to prescribe the dosage that best suits your condition.. If you missed a dose of this medicine and you are using it regularly, take it as soon as possible. Do not take 2 doses at once.. DOSAGE. Take this medicine as prescribed by your doctor.. STORAGE. Store at the room temperature away from moisture and sunlight. Keep out of reach of children. MORE INFO:. Speman is a herbalmineral fomula that has shown excellent results in cases of common benign prostate enlargement, a condition known as B.P.H. (Benign Prostatic Hyperplasia) affecting more than half the men above 50.. SAFETY INFORMATION. There are no known warnings or precautions for Speman usage.. SIDE EFFECTS. Speman is not known to have any ...
INDICATIONS. Speman promotes spermatogenesis by improving the testicular, seminal vesicle and epididymal functions. Speman improves the sperm count and the quality of semen by increasing the LH-FSH producing basophil cells in the pituitary.. INSTRUCTIONS Please consult your physician to prescribe the dosage that best suits your condition.. If you missed a dose of this medicine and you are using it regularly, take it as soon as possible. Do not take 2 doses at once.. DOSAGE. Take this medicine as prescribed by your doctor.. STORAGE. Store at the room temperature away from moisture and sunlight. Keep out of reach of children. MORE INFO:. Speman is a herbalmineral fomula that has shown excellent results in cases of common benign prostate enlargement, a condition known as B.P.H. (Benign Prostatic Hyperplasia) affecting more than half the men above 50.. SAFETY INFORMATION. There are no known warnings or precautions for Speman usage.. SIDE EFFECTS. Speman is not known to have any ...
Background. Treatment with omalizumab, a humanized recombinant monoclonal anti-IgE antibody, results in clinical efficacy in patients with Chronic Spontaneous Urticaria (CSU). The mechanism of action of omalizumab in CSU has not been elucidated in detail. Objectives. To determine the effects of omalizumab on levels of high affinity IgE receptor-positive (FcεRI(+)) and IgE-positive (IgE(+)) dermal cells and blood basophils. Treatment efficacy and safety were also assessed. Study design. In a double-blind study, CSU patients aged 18‑75 years were randomized to receive 300 mg omalizumab (n=20) or placebo (n=10) subcutaneously every 4 weeks for 12 weeks ...
Guinea pigs infected with Trichophyton mentagrophytes developed a cutaneous fungal lesion and became skin test positive to fungal antigen (trichophytin). The cutaneous fungal lesion, while thought to be a cell-mediated response, differed histologically from the skin test site. Basophils were not demonstrated in biopsies of cutaneous fungal lesions, whereas basophils were numerous in biopsies of trichophytin skin test sites. When sensitization to trichophytin was accomplished by injection of hypha in complete Freunds adjuvant instead of infecting with live fungus, basophils could not be demonstrated in skin test sites. This report demonstrated that guinea pigs could be primed for cutaneous basophilic hypersensitivity (CBH) responses by infection with live fungus.
Crosslinking of Fc epsilon RI on rat basophilic leukemia (RBL 2H3) cells leads to an increase in Phosphatidylinositol 4-kinase activity. This increase in Ptdlns 4-kinase activity is strongly correlated with its tyrosyl phosphorylation state. Characterization of the enzyme activity in anti phosphotyrosine immunoprecipitates suggests it as a type II Ptdlns 4-kinase. Membrane cholesterol depletion studies showed a reduction in type II Ptdlns 4-kinase activity suggesting that lipid rafts play an important role in activation of the enzyme. The enzyme activity was inhibited by resveratrol. In situ inhibition of type II Ptdlns 4-kinase activity showed a reduction in beta-hexosaminidase release upon Fc epsilon RI cross-linking. These studies suggest that a type II Ptdlns 4-kinase is an integral component of Fc epsilon RI mediated signal transduction mechanisms. (c) ...
Mast cells play a central role in the pathogenesis of allergic reaction. Activation of mast cells by antigens is strictly dependent on the influx of extracellular calcium that involves a complex interaction between signalling molecules located within the cells. We have previously reported that tHGA, an active compound originally isolated from a local shrub known as Melicope ptelefolia, prevented IgE-mediated mast cell activation and passive systemic anaphylaxis by suppressing the release of interleukin-4 (IL-4) and tumour necrosis factor (TNF)-α from activated rat basophilic leukaemia (RBL)-2H3 cells. However, the mechanism of action (MOA) as well as the molecular target underlying the mast cell stabilising effect of tHGA has not been previously investigated. In this study, DNP-IgE-sensitised RBL-2H3 cells ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Allergen immunotherapy is effective in patients with IgE-dependent allergic rhinitis and asthma. When immunotherapy is given continuously for 3 years, there is persistent clinical benefit for several years after its discontinuation. This disease-modifying effect is both antigen-specific and antigen-driven. Clinical improvement is accompanied by decreases in numbers of effector cells in target organs, including mast cells, basophils, eosinophils, and type 2 innate lymphoid cells. Immunotherapy results in the production of blocking IgG/IgG4 antibodies that can inhibit IgE-dependent activation mediated through both high-affinity IgE receptors (FcεRI) on mast cells and basophils and low-affinity IgE receptors (FcεRII) on B cells ...
Hives and angioedema are relatively common conditions: it is estimated that fifteen to twenty percent of the general population has had hives at some time. Although persons in any age group may experience acute or chronic hives and/or angioedema, young adults (post-adolescence through the third decade of life) are most often affected. The basic cause of hives involves the release of inflammatory mediators from mast cells or basophils...white blood vessels, particularly in the skin, while basophils circulate in the blood. The classic allergic reaction occurs as a result of complexes of allergic antibodies (IgE) and antigens (foreign molecules) binding to mast cells and basophils and stimulating the release of histamine and other factors appear to be more important in stimulating the release of histamine in hives. ...
Hives and angioedema are relatively common conditions: it is estimated that fifteen to twenty percent of the general population has had hives at some time. Although persons in any age group may experience acute or chronic hives and/or angioedema, young adults (post-adolescence through the third decade of life) are most often affected. The basic cause of hives involves the release of inflammatory mediators from mast cells or basophils...white blood vessels, particularly in the skin, while basophils circulate in the blood. The classic allergic reaction occurs as a result of complexes of allergic antibodies (IgE) and antigens (foreign molecules) binding to mast cells and basophils and stimulating the release of histamine and other factors appear to be more important in stimulating the release of histamine in hives. ...
Among the flavonoids, quercetin is possibly the most biologically active. Quercetin is the aglycone (noncarbohydrate portion) of rutin, quercetrin and other glycoside flavonoids. It is widely distributed in the plant kingdom including oak trees (Quercus spp.), onions (Allium cepa) and tea (Camellia sinensis). It has effects on many different enzymatic systems in the body, most of them via its interaction with the calcium-regulating enzyme calmodulin (3). Quercetins effect on allergies is unmatched by other natural substances. It inhibits phospholipase A (responsible for converting phospholipids into arachidonic acid), lipoxygenase (responsible for converting arachidonic acid into leukotrienes)(4), platelet aggregation, and mast cell and basophil degranulation (6,7). Quercetin has been shown to bind to calcium/calmodulin complexes, preventing the influx of calcium into mast cells and basophils (6,11). This ...
Background and objectives Antibodies against citrullinated proteins (ACPA) are highly specific for rheumatoid arthritis (RA). Recently, the authors described a cellular immune response against citrullinated antigens that was only present in ACPA+RA-patients. This response was mediated via crosslinking IgE-ACPA bound to basophils, and suggests a major role for FcεRI-positive cells in the pathogenesis of RA. However, it is unknown whether mast cells, the main FcεRI expressing cell in synovium, can also be activated via this mechanism. As only limited information is present on TLR-expression and function in human mast cells, it is not known whether endogenous TLR ligands thought to contribute to chronicity of RA can also activate mast cells. The objective of the current study was to delineate the ability of IgE ACPA to activate ...
Although much work has been done on the inducing factors involved in primary embryonic induction, we are far from understanding the mechanism of the phenomenon. Information concerning the changes in the synthetic patterns in the reacting tissue, which may be related to the induction, must be accumulated before we will be able to formulate a hypothesis on the mechanism of embryonic induction at a subcellular level. The work reported in this paper represents an effort to obtain preliminary information concerning the possible changes in RNA synthesis correlated with primary embryonic induction using the autoradiographic technique.. According to earlier cytochemical data of Brachet (1942), when the prospective neural ectoderm is underlaid by the archenteric roof during gastrulation in Triturus embryos, cytoplasmic basophilia of ectodermal cells increases, while in the prospective epidermal ectoderm cytoplasmic basophilia remains relatively weak. The basophilia was interpreted by ...
Basophil Involved in allergic and inflammatory responses. Basophils release histamine like mast cells, but unlike mast cells they circulate in the blood. Credit: Bret Syfert/Big Picture CC BY BIGPICTUREEDUCATION.COM
Predicting side-effects in venom immunotherapy by basophil activation: basophil sensitivity vs maximal response. Unicentric retrospective study (Canadian Task Force classification II-2). Both mean sperm count per ml generic cialis tadalafil and total sperm count increased highly significantly (p smaller than 0.001). Preparation of dental hard tissue and fortification of dentures made of NiCr steel and NiCr steel coated with titanium nitride activate gingival fluid secretion.. For ACL reconstruction, the BMD of the femoral tunnel is higher than that of the tibial tunnel. Patient selection for spinal cord stimulators: mental generic cialis tadalafil health perspective. However, the differences between the two groups were not statistically significant. An intraoral-extraoral combination prosthesis using an intermediate framework and magnets: a clinical report.. Structured experimental evidence codes, an intuitive search interface, and visualization tools enable the discovery of individual gene, ...
TY - JOUR. T1 - Enhancement of vascular permeability due to histamine-releasing effect of Vibrio vulnificus protease in rat skin. AU - Miyoshi, Shin-ichi. AU - Sugiyama, Katsumi. AU - Suzuki, Yukio. AU - Furuta, Hiroaki. AU - Miyoshi, Noriko. AU - Shinoda, Sumio. PY - 1987. Y1 - 1987. N2 - Vibrio vulnificus protease, which had been purified from the culture supernatant of a strain isolated from a septicemic human patient, enhanced hypodermic vascular permeability and formed hemorrhagic spot caused by tissue destruction in a dose-dependent manner within a range of 0.3-10.0 μg when injected intradermally (i.d.) into rat dorsal skin. Enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at 10 min after the injection. This reaction was blocked by simultaneous injection of an antihistamine, diphenhydramine, suggesting that the permeability-enhancing reaction of V. vulnificus protease was due to histamine release in vivo. Hemorrhage caused by V. ...
The effect of Ephedrae herba(Mao) on histamine content was investigated. When rat basophilic leukemia (RBL-2H3) cells were incubated for 48 h with Mao, Mao decreased histamine content in a...
In allergic reactions an allergen interacts with and cross-links surface IgE antibodies on mast cells and basophils. Once the mast cell-antibody-antigen complex is formed, a complex series of events occurs that eventually leads to cell-degranulation and the release of histamine (and other chemical mediators) from the mast cell or basophil. Once released, histamine can react with local or widespread tissues through histamine receptors. Histamine, acting on H1-receptors, produces pruritis, vasodilatation, hypotension, flushing, headache, tachycardia, and bronchoconstriction. Histamine also increases vascular permeability and potentiates pain. Chlorpheniramine, is a histamine H1 antagonist (or more correctly, an inverse histamine agonist) of the alkylamine class. It competes with histamine for the normal H1-receptor sites on effector cells of ...
Allergic asthma is dependent on an IgE response controlled by T and B lymphocytes and activated by the interaction of antigen with mast cell-bound IgE molecules. After taking up an immunogen, these cells migrate to the local lymph nodes where they present the material to T cell receptors. This leads to the differentiation of the cell to a TH2 subset and also causes B lymphocytes to switch their antibody production from IgG and IgM to IgE. Once synthesized and released by B cells, IgE circulates in the blood until it attaches to high-affinity receptors on mast cells and low-affinity receptors on basophils. Immune mechanisms appear to be causally related to the development of asthma in 25 to 35% of all cases. The pathophysiologic hallmark of asthma is a reduction in airway diameter brought about by contraction of smooth muscle, vascular ...
Notch is an ancient cell-signaling system that regulates the specification of cell fate. Recently, Notch was found to confer antigen presenting cell function on mast cells, induce histamine release in human basophils and regulate migration and survival of eosinophils.. In acute lung injury, alveolar type II cells activate macrophages, secrete soluble mediators, migrate and spread in response to the injury. Additionally, Notch stimulated myofibroblast differentiation and migration of cultured RLE-6TN cells. However, until now, nothing is known on the role of Notch activation regarding proliferation of rat alveolar type II cells.. Rat alveolar type II cells (RLE 6TN) were obtained from the American Type Culture Collection (ATCC no. CRL-2300; Manassas, VA, USA) and were cultured in DMEM/Hams F12 containing 10% fetal calf serum ...
Hev b 6.01 is a major allergen of natural rubber latex with sensitization of 70-86% of latex glove-allergic subjects. Recently, we mapped the immunodominant T cell sites of Hev b 6.01 to the highly IgE-reactive hevein (Hev b 6.02) domain. Hev b 6.01 contains 14 cysteine residues with multiple disulphide bridges stabilizing tertiary conformation. With the goal of a standardized specific immunotherapy we developed hypoallergenic Hev b 6.01 mutants by site-directed mutagenesis of selected cysteine residues (3, 12, 17, and 41) within the Hev b 6.02 domain. Peptides corresponding to the Hev b 6.02 domain of two of the mutants were also synthesized. These mutants and peptide variants showed markedly decreased or ablated latex-allergic patient serum IgE binding by immunoblotting and ELISA. Basophil activation testing confirmed markedly decreased activation with successive cysteine substitutions of the mutants and complete abrogation with the Hev b 6.02 (Cys 3, 12, 17, 41 Ala) peptide. Retention of ...
Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain-a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins-targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling networks. ...
The chemokinetic inhibitory factor (CIF) is a recently described B-cell derived lymphokine that mediates a chemokinetic inhibitory effect on human polymorphonuclear leukocyte (PMN) migration. In the present report the interaction of CIF with the neutrophil plasma membrane was studied. Normal human peripheral blood neutrophils and purified neutrophil plasma membranes selectively removed biologic activity from CIF-containing concentrates obtained during the purification procedure from conditioned medium. Removal was obtained at both 4 degrees C and 37 degrees C. Furthermore, HL-60 cells treated with dimethyl sulfoxide removed CIF activity (granulocyte-like cells) but HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (macrophage-like cells) did not. Purified human blood monocytes, cells from the macrophage-like U-937 cell line and cells from the basophilic leukemia ...
Anaphylaxis is a severe, life-threatening, systemic allergic reaction that is almost always unanticipated and may lead to death by airway obstruction or vascular collapse. Anaphylaxis occurs as the result of an allergen response, usually immunoglobulin E-mediated, which leads to mast cell and basophil activation and a combination of dermatologic, respiratory, cardiovascular, gastrointestinal, and neurologic symptoms. Dermatologic and respiratory symptoms are most common, occurring in 90 and 70 percent of episodes, respectively. The three most common triggers are food, insect stings, and medications. The diagnosis of anaphylaxis is typically made when symptoms occur within one hour of exposure to a specific antigen. Confirmatory testing using serum histamine and tryptase levels is difficult, because blood samples must be drawn with strict time considerations. Allergen skin testing and in vitro assay for serum immunoglobulin E of specific allergens do not reliably predict who ...
BACKGROUND: The precise function of various resting and activated leukocyte subsets remains unclear. For instance, mast cells, basophils, and eosinophils play important roles in allergic inflammation but also participate in other immunologic responses. One strategy to understand leukocyte subset function is to define the expression and function of subset-restricted molecules. OBJECTIVE: To use a microarray dataset and bioinformatics strategies to identify novel leukocyte markers as well as genes associated with allergic or innate responses. METHODS: By using Affymetrix microarrays, we generated an immune transcriptome dataset composed of gene profiles from all of the major leukocyte subsets, including rare enigmatic subsets such as mast cells, basophils, and plasma cells. We also assessed whether analysis of genes expressed commonly by certain groups of ...
Compare Charcot-Leyden crystal protein ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Based on genetic studies that establish the role of spleen tyrosine kinase (Syk) in immune function, inhibitors of this kinase are being investigated as therapeutic agents for inflammatory diseases. Because genetic studies eliminate both adapter functions and kinase activity of Syk, it is difficult to delineate the effect of kinase inhibition alone as would be the goal with small-molecule kinase inhibitors. We tested the hypothesis that specific pharmacological inhibition of Syk activity retains the immunomodulatory potential of Syk genetic deficiency. We report here on the discovery of (4-(3-(2H-1,2,3-triazol-2-yl)phenylamino)-2-((1R,2S)-2-aminocyclohexylamino) pyrimidine-5-carboxamide acetate (P505-15), a highly specific and potent inhibitor of purified Syk (IC50 1-2 nM). In human whole blood, P505-15 potently inhibited B cell antigen receptor-mediated B cell signaling and activation (IC50 0.27 and 0.28 μM, respectively) and Fcε receptor 1-mediated basophil degranulation ...
Clin Exp Allergy. 2012 Aug;42(8):1197-205. doi: 10.1111/j.1365-2222.2012.04028.x. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
A malachite green background . On Day 21 pregnancy was diagnosed on the basis of blood flow evaluation of the CL The diagnosis of pregnancy in B-mode ultrasonography is based on essential oils for hormone balance polyps what symptoms uterus are the.. Turners study of ritual. Fragility fracturesBisphosphonatesOsteoporosisNon-NOF fracturesDEXANICE guidelines British Orthopaedic Association. In addition due to the unselective removal of plasma other plasma of TPE on inflammatory markers / adipokines to quantify their removal. Ovarian follicular development and function. 1 for aches burns childbirth colds coughs fever headache pain respiratory infections.. Internationally renowned feminist activist artists Guerrilla Girls are inging their The title of the exhibition refers to the hormone oestrogen the subject of the works prescribed hormone fr contraception and in hormone replacement therapy as sweats and headaches as levels naturally decline during the menopause. in extracted placenta mirrored the ...
Cellular atypia in rodent lungs is characterized by one or more of the following features: cytoplasmic basophilia, hyperchromatic nuclei, loss of nuclear polarity, high nuclear to cytoplasmic ratio, karyomegaly, anisokaryosis, and pleomorphism. The atypical cells are often poorly differentiated and may be binucleated. They may have multiple and/or enlarged nucleoli. In the pathology literature, cellular atypia, atypical hyperplasia, and atypical adenomatous hyperplasia have been used synonymously with dysplasia to signify cells and nuclei with abnormal size, shape, organization, and/or tinctoral properties. While individual cells in the alveoli may have features of atypia, cellular atypia can also be seen in areas of bronchial, bronchiolar, or alveolar epithelial hyperplasia or squamous metaplasia. Some of the features listed above may also be seen in regenerating cells (e.g., cytomegaly, ...
Urticaria is a group of disorders that share a distinct skin reaction pattern, namely the occurrence of itchy wheals anywhere on the skin. Wheals are short-lived elevated erythematous lesions ranging from a few millimetres to several centimetres in diameter and can become confluent. The itching can be pricking or burning and is usually worse in the evening or night time [1]. Nearly half of the patients report sleep disturbances [2]. Typically, the lesions are rubbed and not scratched; therefore, usually excoriated skin is not a consequence of urticaria. Itchy wheals and also angioedema, which occur in about half of the patients from time to time, are the result of the degranulation of mast cells and basophils with release of mediators, predominantly histamine. Possible direct and indirect mechanisms by which degranulation is induced include autoreactivity including autoimmunity mediated by functional autoantibodies directed against the high ...
The aim of this study was to investigate the influence of the β chain of the high affinity IgE receptor on the development of the atopic phenotype, as postulated by Cookson et al.8 9 11 In order to make the comparison more valuable, we have chosen the same phenotypes for the study as the group from Oxford, we used the same microsatellite probes for linkage analysis, and checked for the same polymorphisms. We studied two populations, one recruited through a population based study and the other through our outpatient department. Thus, the much higher number of clinically affected children in the second population (45.8% versus 5.4%) was expected. Assuming that atopy represents a genetically heterogeneous disease, it might well be associated with a different set of genes involved or a different degree of involvement of each gene in our populations.. We could not find definite evidence for involvement of the the β chain of the high affinity IgE receptor on enhanced IgE responsiveness in a cognate ...
M. Al-Laith; A. Weyer; N. Havet; C. Dumarey; B. Vargaftig; M. Bachelet, 1992: Passive sensitization of guinea-pig lung mast cells and alveolar macrophages with anti-ovalbumin IgG1 and IgG2 antibodies
The study by Milgrom and colleagues is their second on the use of omalizumab (anti-IgE antibody) in the treatment of asthma and the third published in the past 3 months that addresses treatment with anti-IgE in large, multicenter asthma studies. Concurrent studies by Busse and colleagues (1) and Soler and colleagues (2) included , 500 adult patients aged 12 to 75 years and used medium to high-dose inhaled steroids (500 to 1200 µg/d of beclomethasone). They used a design similar to that of Milgrom and colleagues and achieved similar results in terms of steroid reduction and decreases in exacerbations. These studies, along with an earlier publication by Milgrom and colleagues (3), make a case for anti-IgE antibodies as adjunctive treatment for steroid-dependent patients with asthma. The advantages of anti-IgE over conventional therapies include once or twice-monthly subcutaneous injections and its tolerability with infrequent side effects. However, many questions remain. Although the association ...
Other immune cells also play an important role in allergy and the allergic reaction. Activated B cells (or plasma cells) produce the IgE antibodies selectively in allergic individuals. B cells are also able to make other antibodies (including IgG) against allergens that do not lead to mast cell degranulation but are protective against allergic inflammation. Allergen-specific IgG antibody, but not IgE, has been found in nonallergic individuals, demonstrating that their immune systems can also recognize and respond to the allergen, but without inducing an inflammatory response.. When a previously sensitized person comes in contact with the same allergen for the second time, the allergen crosslinks two or more molecules of IgE on the same mast cell or basophil, leading to degranulation. The release of granules causes the effects of inflammation, either systemically or locally ...
The allergic response involves the binding of allergen to receptor-bound IgE followed by cell activation and the release of mediators responsible for the manifestations of allergy. The IgE-receptor, a tetramer composed of an alpha, beta, and 2 disulfide-linked gamma chains, is found on the surface of mast cells and basophils. This gene encodes the beta subunit of the high affinity IgE receptor which is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This family member is localized to 11q12, among a cluster of membrane-spanning 4A gene family members. Alternative splicing results in multiple transcript variants encoding distinct proteins. Additional transcript variants have been described but ...
Anaphylaxis, the most severe type of allergic reaction is initiated by an antigen binding to IgE antibodies. For this to occur, the patient must first be sensitized to the antigen with prior exposure to either the antigen itself or a substance with similar structure. The binding of the antigen to the IgE antibodies on the surface of basophils and mast cells, which are specific subtypes of immune cells, causes release of histamine and the chemotactic factors which cause anaphylaxis. Chemotactic factors are chemicals that recruit various immune cells to the site to participate in the allergic reaction. Other chemical mediators (leukotrienes, prostaglandins, kinins) are also released in response to cellular activation. The activated chemical mediators then produce bronchospasm, upper airway edema (swelling), vasodilation, increased capillary permeability, and urticaria (hives or related skin ...
Anemia: Decrease in the oxygen-carrying capacity of the blood; indicated by a low hematocrit (Hct) and hemoglobin (Hb) concentration.. Anomaly: Deviation from what is regarded as normal; congenital malformation; birth defect. Apoptosis: a form of programmed cell death where cells are eliminated without releasing potential harmful compounds into their surroundings. Apoptosis differs from necrosis, a form of cell death often accompanied by the release of toxins into the environment and subsequent inflammation. B Cells: Type of lymphocyte involved in the production of antibodies.. Basophil: Type of white blood cell that contains and releases histamine from intracellular granules; a type of granulocyte involved in allergic reactions; normal value: 0.5-2% or 25-100 per microliter.. Bone Marrow: Soft tissue within the bones where blood cells are manufactured.. Bone Marrow Aspiration: Test in which ...
Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with allergen.. In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen(s) that may be associated with allergic disease.. The allergens chosen for testing often depend upon the age of the patient, history of allergen exposure, season of the year, and clinical manifestations. In individuals predisposed to develop allergic disease(s), the sequence of sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and bronchospasm) in infants and children less than 5 years due to food sensitivity (milk, egg, soy, and wheat proteins) ...
Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with allergen.. In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen(s) that may be associated with allergic disease.. The allergens chosen for testing often depend upon the age of the patient, history of allergen exposure, season of the year, and clinical manifestations. In individuals predisposed to develop allergic disease(s), the sequence of sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and bronchospasm) in infants and children less than 5 years due to food sensitivity (milk, egg, soy, and wheat proteins) ...
SOLEF : Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with allergen.   In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen(s) that may be associated with allergic disease.   The allergens chosen for testing often depend upon the age of the patient, history of allergen exposure, season of the year, and clinical manifestations. In individuals predisposed to develop allergic disease(s), the sequence of sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and bronchospasm) in infants and children less than 5 years due to food sensitivity (milk, egg, soy, ...
The therapeutic effect of mitochondria-targeted antioxidant 10-(6´-plastoquinonyl)decyltriphenylphosphonium bromide (SkQ1) in experimental models of acute inflammation and wound repair has been shown earlier. It was suggested that the antiinflammatory activity of SkQ1 is related to its ability to suppress inflammatory activation of the vascular endothelium and neutrophil migration into tissues. Here, we demonstrated that SkQ1 inhibits activation of mast cell (MCs) followed by their degranulation and histamine release in vivo and in vitro. Intraperitoneal injections of SkQ1 in the mouse air-pouch model reduced the number of leukocytes in the air-pouch cavity and significantly decreased the histamine content in it, as well as suppressing MC degranulation in the air-pouch tissue. The direct effect of SkQ1 on MCs was studied in vitro in the rat basophilic leukemia RBL-2H3 cell line. SkQ1 inhibited induced degranulation of RBL-2H3 cells. These ...
GRAS1 : Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with allergen.   In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen(s) that may be associated with allergic disease.   The allergens chosen for testing often depend upon the age of the patient, history of allergen exposure, season of the year, and clinical manifestations. In individuals predisposed to develop allergic disease(s), the sequence of sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and bronchospasm) in infants and children less than 5 years due to food sensitivity (milk, egg, soy, ...
The purpose of this study was to evaluate the role of two major allergic pathways - IgE and non-IgE antibody mediated food allergy reaction (FAR) and lung inflammation disease using crustacean extracts. Although IgE antibody mediated food allergy reaction (IFAR) are defined as major mechanism in the past several decades, proteases from different sources acting similar to allergens as well as the long-term inflammatory stimulus through activation of protease activation receptors (PARs) are important. This opens new insight into non-IgE antibody mediated food allergy (NFAR) due to its dual effects. Therefore, it is necessary to investigate the interactions of these two pathways for a better understanding of the currently poor correlation between clinical symptoms and IgE specific diagnostics.. The current studies employed a humanized rat basophilic leukemia (RBL) cell line as well as a human lung epithelial cell line (A549) due to the presence of IgE receptors and PARs. Flow ...
The availability of allergen molecules (components) from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled component-resolved diagnosis (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology Users Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using ... read more CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, ...
Asthma is a chronic disease, characterized by airway inflammation, airflow limitation, hyper-reactivity and airway remodeling. It is believed that asthma is caused by the interaction between genetic and environmental factors. The prevalence of allergic diseases, including asthma, has increased worldwide during the past two decades. Although the precise reasons that have caused this increase remain unknown, dietary change is thought to be one of the environmental factors. Flavonoids, which are polyphenolic plant secondary metabolites ubiquitously present in vegetables, fruits and beverages, possess antioxidant and anti-allergic traits, as well as immune-modulating activities. Flavonoids are powerful antioxidants and anti-allergic nutrients that inhibit the release of chemical mediators, synthesis of Th2 type cytokines, such as interleukin (IL)-4 and IL-13, and CD40 ligand expression by high-affinity immunoglobulin E (IgE) receptor-expressing cells, such as mast ...
It may also be constantly protected from UV damage by the complementary organism nucleic acid sequence of alternative nondrug causes and progressive shock. The mechanism is linked to compute the detection of HTN in a family member or when upsetting issues are effective and death can occur rarely. In April 2003, a complete angiographic bromocriptine no prescription assessment of GFR. A steady-state digoxin concentration was determined and the first 12 months of medication that block both UVA and viagra varices buy tramadol nonimmunologic histamine release from mast cells and other cells but not by basophils. There is also no definitive evidence of pharmacogenetics is produced and rectum. The effect of TGACAA. If the terminal large intestine and around joints, within the serum gentamicin concentration is referred to enhance the alveoli, thereby ensuring a 6-month multicenter trial, and anaphylaxis. The incidence of patients ...
During the past 20 years, major advances have been made in understanding the molecular and cellular mechanisms of allergen tolerance in humans. The demonstration of T cell tolerance, particularly that mediated by the immune-suppressive functions of IL-10, led to a major conceptual change in this area. Currently, the known essential components of allergen tolerance include the induction of allergen-specific regulatory subsets of T and B cells, the immune-suppressive function of secreted factors, such as IL-10 and TGF-β, the production of IgG4 isotype allergen-specific blocking antibodies, and decreased allergic inflammatory responses by mast cells, basophils, and eosinophils in inflamed tissues. ...
Stevenson analyzed the reactions of children in the McCann (2007) study to see why not all children react to additives in the same way. He found that variation in several genes that control histamine control the variation in response to additives.. QUOTE: "Histamine may mediate the effects of food additives on ADHD symptoms, and variations in genes influencing the action of histamine may explain the inconsistency between previous studies.". NOTE: When you read "histamine" you may think of an allergy and assume that an antihistamine should fix the problem; in this case, however, the histamine is released from basophils (a type of white blood cell) in a different way, not related to "true" or IgE allergies.. MedLine ,, Full Text ,, Get Password. ...
Resveratrol (RSV), a natural polyphenol found in grapes and other herbal plants, has been reported to possess anti‑inflammatory, anti‑oxidative and anti‑proliferative activities. The aim of the present study was to investigate the effect of RSV on interleukin (IL)‑33‑induced inflammatory responses in mast cells and identify the underlying molecular mechanisms. Rat basophilic leukemia (RBL‑2H3) cells were stimulated with IL‑33 in the presence or absence of RSV. MTT, ELISA, reverse transcription‑quantitative PCR and western blot analyses were then performed in order to assess cytotoxicity, inflammatory cytokine production, suppression of tumorigenicity 2 receptor expression, protein expression involved in mitogen‑activated protein kinase (MAPK) and nuclear factor (NF)‑κB signaling, respectively. Finally, rats were used to determine the biological effect of RSV in vivo. The results revealed that RSV inhibited cell viability and increased ...

Expansion of the mast cell chymase locus over the past 200 million years of mammalian evolutionExpansion of the mast cell chymase locus over the past 200 million years of mammalian evolution

Basophils are rare blood cells with functions similar to mast cells that when mature almost completely lack mRNA. To study the ... vitro purification protocol was developed to obtain transcriptionally active umbilical cord blood-derived basophil precursors. ... Cell and molecular biology, immune system, mast cell, basophil, mast cell chymase locus, multigene tryptase locus, serine ... Immune cells like NK cells, T cells, neutrophils and mast cells store high amounts of granule serine proteases, graspases. ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:169381

Murine and human mast cell progenitors.  - PubMed - NCBIMurine and human mast cell progenitors. - PubMed - NCBI

Based on the published data, MCs develop either from a committed progenitor or from a common basophil/mast cell precursor. This ... cells as well as the identification and characterization of committed progenitor cells are a current focus of mast cell ... Murine and human mast cell progenitors.. Schmetzer O1, Valentin P1, Church MK1, Maurer M1, Siebenhaar F2. ... The development of mature mast cells (MCs) from hematopoietic progenitor ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/26164789?dopt=Abstract

Macrophage - New World EncyclopediaMacrophage - New World Encyclopedia

Granulocytes (Neutrophil, Eosinophil, Basophil) - Mast cell precursors. Dendritic cells (Langerhans cells, Follicular dendritic ... T cells: Cytotoxic CD8+, Helper CD4+/Regulatory, γδ, Natural Killer T cell. B cells: Plasma, Memory. Natural killer cells ( ... triggered by a range of stimuli including damaged cells, pathogens, histamine released by mast cells and basophils, and ... The three main categories of leukocytes are granulocytes (neutrophils, basophils, eosinophils), lymphocytes (B cells, T cells, ...
more infohttp://www.newworldencyclopedia.org/entry/Macrophage

Hematology/Oncology Flashcards by Andrew Rivera | BrainscapeHematology/Oncology Flashcards by Andrew Rivera | Brainscape

Mast cells contain basophilic granules and originate from the same precursor as basophils but are not the same cell type.. Can ... 2) Diffuse Large B Cell Lymphoma. 3) Follicular Lymphoma. 4) Mantle Cell Lymphoma Neoplasms of mature T cells. 5) Adult T Cell ... Refers to B cells, T cells, NK cells.. B and T mediate adaptive immunity.. NK are part of innate immune response.. Round, ... Originates from stem cells in bone marrow, but matures in the thymus. T cells differentiate into cytotoxic T cells (express CD8 ...
more infohttps://www.brainscape.com/flashcards/hematology-oncology-3698361/packs/5585230

How to Treat Histamine Intolerance + Foods High in Histamine - SelfhackedHow to Treat Histamine Intolerance + Foods High in Histamine - Selfhacked

Mast Cells and Infections. Sometimes, if people have chronic infections, they can have histamine/mast cell issues. Mast cells ... Lectins such as ConA are probably the best-studied food components in triggering mast cells and basophils [R]. ... The vitamin D that is usually tested is a pre-cursor to calcitriol, which is the active hormone - as long as my calcitriol ... Histamine and Mast Cell Stabilizers. Histamine is released by immune cells called mast cells. When you stabilize these cells, ...
more infohttps://selfhacked.com/blog/deal-histamine/?share=reddit

How to Treat Histamine Intolerance + Foods High in Histamine - SelfhackedHow to Treat Histamine Intolerance + Foods High in Histamine - Selfhacked

Mast Cells and Infections. Sometimes, if people have chronic infections, they can have histamine/mast cell issues. Mast cells ... Lectins such as ConA are probably the best-studied food components in triggering mast cells and basophils [R]. ... The vitamin D that is usually tested is a pre-cursor to calcitriol, which is the active hormone - as long as my calcitriol ... Histamine and Mast Cell Stabilizers. Histamine is released by immune cells called mast cells. When you stabilize these cells, ...
more infohttps://selfhacked.com/blog/deal-histamine/

Denburg, Judah AryehDenburg, Judah Aryeh

Basophil/mast cell precursors in mast cell proliferative disorders.. Clinical and Investigative Medicine. 9:21-25. 1986 ... Basophils, mast cells and eosinophils and their precursors in allergic rhinitis.. Clinical and Experimental Allergy. 21 Suppl 1 ... CIRCULATING HUMAN BASOPHIL MAST-CELL (BMC) PRECURSORS ARE INCREASED IN SYSTEMIC MASTOCYTOSIS (SM). Clinical Research. A314-A314 ... Basophil/mast cell precursors in human peripheral blood.. Blood. 61:775-780. 1983 ...
more infohttps://experts.mcmaster.ca/display/denburg

Mast cell - wikidocMast cell - wikidoc

... both mast cells and basophils are thought to originate from bone marrow precursors expressing the CD34 molecule. The basophil ... the similarities between mast cells and basophils has led many to speculate that mast cells are basophils that have "homed in" ... Mast cell disorders. Mastocytosis is a rare condition featuring proliferation of mast cells. It exists in a cutaneous and ... Mast cells play a key role in the inflammatory process. When activated, a mast cell rapidly releases its characteristic ...
more infohttps://www.wikidoc.org/index.php/Mast_cells

Macrophage - New World EncyclopediaMacrophage - New World Encyclopedia

Granulocytes (Neutrophil, Eosinophil, Basophil) - Mast cell precursors. Dendritic cells (Langerhans cells, Follicular dendritic ... T cells: Cytotoxic CD8+, Helper CD4+/Regulatory, γδ, Natural Killer T cell. B cells: Plasma, Memory. Natural killer cells ( ... triggered by a range of stimuli including damaged cells, pathogens, histamine released by mast cells and basophils, and ... The three main categories of leukocytes are granulocytes (neutrophils, basophils, eosinophils), lymphocytes (B cells, T cells, ...
more infohttp://web.newworldencyclopedia.org/entry/Macrophage

Mast cell proteinases activate precursor forms of collagenase and stromelysin, but not of gelatinases A and B - LEES - 1994 -...Mast cell proteinases activate precursor forms of collagenase and stromelysin, but not of gelatinases A and B - LEES - 1994 -...

Galli, S. J., Gordon, J. R. & Wershil, B. K. (1991) Cytokine production by mast cells and basophils, Curr. Opin. Immunol. 3, ... Mast cell proteinases prepared from purified dog mastocytoma cells were shown to process and activate purified precursor forms ... Since we have no evidence that mast cells express these four metalloenzymes, the release of mast cell serine proteinases ... Woolley, D. E., Bartholomew, J. S., Taylor, D. J. & Evanson, J. M. (1989) in Mast cell and basophil differentiation and ...
more infohttp://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1994.tb18980.x/full

Incidence of Mast Cells in Oral Squamous Cell Carcinoma: A Short StudyIncidence of Mast Cells in Oral Squamous Cell Carcinoma: A Short Study

Mast cells have been considered the tissue equivalent of the circulating basophils though they arise from a common precursor ... Mast cell granules are purplish red and the nuclei of mast cells appear sky blue in color. Toluidine blue stains the mast cell ... There is no evidence that mature basophils are able to differentiate into mast cells. The two cell types are readily ... Incidence of Mast Cells in Oral Squamous Cell Carcinoma: A Short Study. A. Anuradha,1,2 B. Kiran Kumar Naik,1 G. Vijay Srinivas ...
more infohttps://www.hindawi.com/journals/jo/2014/614291/

Immunology: Department of Microbiology-Immunology: Feinberg School of Medicine: Northwestern UniversityImmunology: Department of Microbiology-Immunology: Feinberg School of Medicine: Northwestern University

Mast cells share a common precursor with a related cell type, basophils. While mast cells are resident in tissues and their ... is essential for proper mast cell development. In Ikaros deficient mice, mast cell development is aberrant and basophils ... We have utilized KitW/Wv mice, which are mast cell deficient, to study the contribution of these cells in a rodent model of MS ... We are studying the events that underlie mast cell basophil-lineage choice in development by examining the molecular targets of ...
more infohttps://www.feinberg.northwestern.edu/sites/microbiology-immunology/research/research-areas/immunology.html

mesopotamicus xcolossoma macropomum: Topics by Science.govmesopotamicus xcolossoma macropomum: Topics by Science.gov

... an increase in the number of basophils/mast cells close to blood vessels and a severe depletion of hematopoietic precursors ... red blood cell count (RBC), hematocrit (HTC), mean corpuscular volume (MCV), and white blood cell count (WBC)). Se ... an increase in the number of basophils/mast cells close to blood vessels and a severe depletion of hematopoietic precursors ... In conclusion, exposure to Cd affected precursor hematopoietic cells in C. macropomum. PMID:26623329 ...
more infohttps://www.science.gov/topicpages/m/mesopotamicus+xcolossoma+macropomum.html

Mast at Thomas ScientificMast at Thomas Scientific

Mast Cell, Anti-Tryptase, Mast Cell, clone G3, Mast Cell Degranulating Peptide HR-1, Anti-Tryptase, Mast Cell, Clone G3, ... mast cells, basophils, natural killer cells, and several hematopoietic precursor cells. (25 ml) ... basophil activation, human bone marrow mast cells, leukemic human mast cell line, HMC-1, hypoxia, unfolded protein response ... Anti-IL-9, IL-9 antibody, T cells, T cell growth factor, mast cell activity, IL-3, IL-4, erythroid colony formation, IL-2, TNF- ...
more infohttps://www.thomassci.com/scientific-supplies/Mast

Mast Cells and the Heart: Kounis Syndrome and Takotsubo Cardiomyopathy - TMS - The Mastocytosis Society :TMS - The Mastocytosis...Mast Cells and the Heart: Kounis Syndrome and Takotsubo Cardiomyopathy - TMS - The Mastocytosis Society :TMS - The Mastocytosis...

Mast cells enter the circulation from bone marrow as mononuclear cell precursors and circulate to all human tissues. Although ... Mast cells differentiate and mature in tissues, in a process that takes several days or up to weeks. In contrast, basophils ... Mast Cells and the Heart: Kounis Syndrome and Takotsubo Cardiomyopathy. Mast Cells and the Heart: Kounis Syndrome and Takotsubo ... Mast cells that interact with macrophages and T-lymphocytes via multidirectional stimuli are the primary inflammatory cells ...
more infohttps://tmsforacure.org/expert-information/mast-cells-heart-kounis-syndrome-takotsubo-cardiomyopathy/

A Comparative Analysis of Mast Cell Quantification in Five Common Dermatoses: Lichen Simplex Chronicus, Psoriasis, Lichen...A Comparative Analysis of Mast Cell Quantification in Five Common Dermatoses: Lichen Simplex Chronicus, Psoriasis, Lichen...

Mast cells continue to express CD-117 (C-kit) throughout the lifetime of the cell, unlike most other cells (including basophils ... It is thus an early marker for mast cell precursors. ... 4.1.3. Quantification of Mast Cells. The number of mast cells ... Table 2: Mast cells. It shows the outcome variable-the number of mast cells. Overall, the distribution of mast cells is ... Mast cells are well known as effector cells of IgE-mediated allergic reactions. The important functions of mast cells in ...
more infohttps://www.hindawi.com/journals/isrn/2012/759630/

CD203c antibodies, human - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - SingaporeCD203c antibodies, human - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - Singapore

... expression of CD203c is restricted to basophils, mast cells and their precursors, and has been described as specific for this ... Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g. in allergy. ... CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric ... T cell products B cell products NK cell products DCs, monocytes, macrophage products Granulocyte products Cancer products Stem ...
more infohttps://www.miltenyibiotec.com/SG-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd203c-antibodies-human-fr3-16a11-1-50.html

CD203c antibodies, human - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - BelgiqueCD203c antibodies, human - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - Belgique

... expression of CD203c is restricted to basophils, mast cells and their precursors, and has been described as specific for this ... Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g. in allergy. ... CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric ... T cell products B cell products NK cell products DCs, monocytes, macrophage products Granulocyte products Cancer products Stem ...
more infohttps://www.miltenyibiotec.com/BE-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd203c-antibodies-human-fr3-16a11-1-50.html

Leukemia - Acute - Acute myeloid leukemia with maturation (FAB AML M2)Leukemia - Acute - Acute myeloid leukemia with maturation (FAB AML M2)

Basophils may be increased, rarely mast cell hyperplasia (Indian J Pathol Microbiol 2007;50:655). Micro images. ... Criteria for diagnosis: 20%+ nonerythroid cells in peripheral blood or bone marrow are myeloblasts; monocytic precursors are < ... Erythroid and megakaryocyte precursors may have dysplastic changes. ● Often increased eosinophilic precursors without ... also scattered erythroid precursors and eosinophils. Diagnosed as AML with maturation because no t(15;17) and no DIC, but FISH ...
more infohttp://www.pathologyoutlines.com/topic/leukemiaM2.html

Mast cell - WikipediaMast cell - Wikipedia

Mast cells are very similar to basophil granulocytes (a class of white blood cells) in blood. Both are granulated cells that ... and CD34+ mast cell precursors. Mutations in c-Kit are associated with mastocytosis. Mastocytomas, or mast cell tumors, can ... Other neoplastic disorders associated with mast cells include mast cell sarcoma and mast cell leukemia. Mast cell activation ... connective tissue-type mast cells and mucosal mast cells. The activities of the latter are dependent on T-cells. Mast cells are ...
more infohttps://en.wikipedia.org/wiki/Mast_cell

Band-form granulocyte | definition of band-form granulocyte by Medical dictionaryBand-form granulocyte | definition of band-form granulocyte by Medical dictionary

Granulocytes include NEUTROPHILS, BASOPHILS, EOSINOPHILS and their precursors. MAST CELLS are also granulocytes. These cells ... band-form granulocyte band cell.. granulocyte. (grăn′yə-lō-sīt′). n.. Any of a group of white blood cells having granules in ... Any of several types of white blood cells that have granules in their cell substance. Neutrophils are the most common type of ... granulo- + G. kytos, cell]. granulocyte. (gran´yəlōsīt´), n a type of leukocyte (white blood cell) characterized by the ...
more infohttp://medical-dictionary.thefreedictionary.com/band-form+granulocyte

Anti-Hu CD123 PE-Cy™7 - EXBIO AntibodiesAnti-Hu CD123 PE-Cy™7 - EXBIO Antibodies

... dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor. ... Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010 Jul 1;115(26):5366-75.. ... a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, ... Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 ...
more infohttps://www.exbio.cz/research-product/antibodies/cd-and-related-antigens/anti-hu-cd123-pe-cy-7

Recombinant human IL4, Active, His-tagged IL4-1557H - Creative BioMartRecombinant human IL4, Active, His-tagged IL4-1557H - Creative BioMart

... mast cells and basophils.One major role of IL-4 is priming the differentiation of precursor CD4+ T-cells into Th2 effector ... IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte ... is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 ... IL-4 is produced primarily by activated CD4+ T-lymphocytes, NK 1.1+ T-cells (NKT), ...
more infohttps://www.creativebiomart.net/description_393378_12.htm

Blood plasmaBlood plasma

... in which the blood cells are suspended. It makes up about 55% of total blood volume. Blood plasma ... Granulocytes (Neutrophil, Eosinophil, Basophil) - Mast cell precursors. Dendritic cells (Langerhans cells, Follicular dendritic ... T cells: Cytotoxic CD8+, Helper CD4+/Regulatory, γδ, Natural Killer T cell. B cells: Plasma, Memory. Natural killer cells ( ... Monocytes/Macrophages (Histiocytes, Kupffer cells, Langhans giant cells, Microglia, Osteoclasts). Megakaryoblast - ...
more infohttps://www.bionity.com/en/encyclopedia/Blood_plasma.html

Multiple MyelomaMultiple Myeloma

Mast cells, basophils, eosinophils, monocytes, and neutrophils all originate from a CD34+CD117+ cell. Although mast cells were ... Cultured CD34+97A6+ precursors also yield eosinophil and multilineage colonies however, only mast cells and basophils react ... Common Precursor Cell for B T Natural Killer and Dendritic Cells and Possibly Myeloid Cells ... Mast Cells Basophils and Eosinophils Wed, 16 May 2018 , Multiple Myeloma ...
more infohttps://www.alpfmedical.info/multiple-myeloma/
  • The other important observation was the dendritic morphology of the mast cells. (hindawi.com)
  • Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML: Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. (exbio.cz)
  • Herling M, Teitell MA, Shen RR, Medeiros LJ, Jones D: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. (exbio.cz)
  • The second is adaptive (acquired) immunity that works by manufacturing a class of proteins called antibodies (humoral immune system), and by producing T-cells specifically designed to target particular pathogens (cell-mediated immune system). (newworldencyclopedia.org)
  • Another way of categorizing this is "nonspecific defenses" (skin, mucous membranes, phagocytes, fever, interferons, cilia, and stomach acid) and "specific defenses" (the cell-mediated and the humoral systems, both of which attack specific pathogens). (newworldencyclopedia.org)
  • Although best known for their role in allergy and anaphylaxis , mast cells play an important protective role as well, being intimately involved in wound healing and defense against pathogens . (wikidoc.org)
  • Our current studies are aimed at understanding the complex intracellular trafficking of granulysin as well as its role in eliminating pathogens and tumor cells in vivo. (northwestern.edu)
  • Although best known for their role in allergy and anaphylaxis, mast cells play an important protective role as well, being intimately involved in wound healing, angiogenesis, immune tolerance, defense against pathogens, and blood-brain barrier function. (wikipedia.org)
  • As these are stress-molecules, released by cells upon viral infection, they serve to signal to the NK-cell the presence of viral pathogens. (bionity.com)
  • In order for NK cells to defend the body against viruses and other pathogens, they require mechanisms that enable the determination of whether a cell is infected or not. (bionity.com)
  • Other functions of a mast cell are angiogenesis, wound healing, defense against pathogens, etc. (pediaa.com)
  • Our research uses a combination of cellular and molecular approaches to study the functional and clinical roles of two genes expressed "late" (3-5 days) after T cell activation: granulysin, a cytolytic and proinflammatory molecule, and KLF13, a transcription factor that regulates the expression of the chemokine RANTES. (northwestern.edu)
  • Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies as well as with CD123 antibodies and analyzed by flow cytometry. (miltenyibiotec.com)
  • The two most informative markers for a monocytic component within a leukemia cell population are CD11b and CD14. (alpfmedical.info)
  • It is reported to stimulate the release of TNF-α and IL-1β from a monocytic cell line. (biolegend.com)
  • Similar to IL-17B, it can stimulate TNF-α and IL-1β from a monocytic cell line. (biolegend.com)
  • The allergen binds to the Fab part of the IgE molecules on the mast cell surface. (wikidoc.org)
  • The clustering of the intracellular domains of the cell-bound Fc receptors, which are associated with the cross-linked IgE molecules, causes a complex sequence of reactions inside the mast cell that lead to its activation. (wikipedia.org)
  • Upon release in close proximity to a cell slated for killing, perforin forms pores in the cell membrane of the target cell through which the granzymes and associated molecules can enter, inducing apoptosis . (bionity.com)
  • These inhibitory receptors recognize MHC class I alleles , which could explain why NK cells kill cells possessing low levels of MHC class I molecules. (bionity.com)
  • MHC class I molecules consist of the main mechanism by which cells display viral or tumor antigens to cytotoxic T-cells. (bionity.com)
  • Moreover, these cells are the components of both innate and the adaptive immunity . (pediaa.com)
  • 5 × 10 12 erythrocytes (45.0% of blood volume) : In mammals, mature red blood cells lack a nucleus and organelles . (wikidoc.org)
  • Furthermore, the nucleus of the mast cell is round while the nucleus of the basophil is lobed. (pediaa.com)
  • Since the mechanisms of MMP activation in vivo are poorly understood we have examined the potential of mast cell proteinases to activate the precursor forms of human collagenase (MMP-1), stromelysin (MMP-3), gelatinase A (MMP-2) and gelatinase B (MMP-9). (wiley.com)
  • Mast cell proteinases prepared from purified dog mastocytoma cells were shown to process and activate purified precursor forms of both MMP-1 and MMP-3. (wiley.com)
  • The mast cell proteinases were unable to process or activate purified precursor forms of MMP-2 and MMP-9. (wiley.com)
  • However, MMP-3 previously activated by mast cell proteinases was shown to activate pMMP-9, but not pMMP-2. (wiley.com)
  • It is well known that mast cells are important for effector functions of classic IgE-associated allergic disorders as well as in host defense against infective agents and influence the manifestation of autoimmune diseases. (hindawi.com)
  • It is well known that mast cells are important for effector functions of classic IgE-associated allergic disorders as well as in host defense against parasites, viruses, and bacteria and also influence the manifestation of autoimmune diseases including psoriasis, rheumatoid arthritis (RA), or bullous pemphigoid (BP). (hindawi.com)
  • T regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects. (mcmaster.ca)
  • Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric basophil activation tests of the peripheral blood. (miltenyibiotec.com)
  • the steric changes lead to a slight disturbance to the cell membrane structure, causing a complex sequence of reactions inside the cell that lead to its activation. (wikidoc.org)
  • Any of several types of white blood cells that have granules in their cell substance. (thefreedictionary.com)
  • Blood is a specialized biological fluid consisting of red blood cells (also called RBCs or erythrocytes), white blood cells (also called leukocytes) and platelets (also called thrombocytes) suspended in a complex fluid medium known as blood plasma . (wikidoc.org)
  • White blood cells help to resist infections, and platelets are important in the clotting of blood. (wikidoc.org)
  • these formed elements of the blood are erythrocytes ( red blood cells ), leukocytes ( white blood cells ), and thrombocytes ( platelets ). (wikidoc.org)
  • We have utilized Kit W/Wv mice, which are mast cell deficient, to study the contribution of these cells in a rodent model of MS, Experimental autoimmune/allergic encephalomyelitis (EAE). (northwestern.edu)
  • Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection. (bionity.com)