Cell-Penetrating Peptides

Peptides

Cysteamine

Peptide Nucleic Acids

Wasp Venoms

Galanin

Drug Delivery Systems

Cell Membrane Permeability

Drug Carriers

Gene Products, tat

Liposomes

Amino Acid Sequence

Recombinant Fusion Proteins

Cell Membrane

Molecular Sequence Data

Peptide Fragments

Lipid Bilayers

Peptide Library

Carrier Proteins

HeLa Cells

Antimicrobial Cationic Peptides

Peptides, Cyclic

Cell-penetrating peptide

Pepducin

Transcriptome in vivo analysis tag

Wasabi receptor toxin

DMBT1

Transflammation

Hadrucalcin

Vejocalcin

Sanford Burnham Prebys Medical Discovery Institute

Peptide

Helma Wennemers

Maurocalcine

Cyclic peptide

Lipinski's rule of five

Peptide nucleic acid

Gajendra Pal Singh Raghava

Kim Janda

CendR

IRGD

Tat (HIV)

Gold nanoparticles in chemotherapy

Morpholino

Antimicrobial peptides

Spatial transcriptomics

Andreas Bernkop-Schnürch

West Nile fever

Erkki Ruoslahti

Protamine

Glucagon-like peptide-2

Penicillium digitatum

Nephron

Porphyrin

Lyme disease

Antimicrobial

Sphaceloma perseae

Food-entrainable oscillator

Stephen J. Lippard

Potential applications of graphene

Lipid bilayer

Spiroligomer

Radiation therapy

Fluorescent D-amino acids

Sperm chemotaxis

Samir Mitragotri

Intracerebral hemorrhage

CPP

Injection (medicine)

Atopic dermatitis

Triple-stranded DNA

Heterologous expression

Striatum

Hypothetical types of biochemistry

Hapten

Phenylalanine

Chemical ecology

Aphid

Melanocyte

Uptake7

• Scavenger receptor-mediated uptake of cell-penetrating peptide nanocomplexes with oligonucleotides. (ox.ac.uk)
• Cell-penetrating peptides: a re-evaluation of the mechanism of cellular uptake. (nih.gov)
• Cellular uptake of unconjugated TAT peptide involves clathrin-dependent endocytosis and heparan sulfate receptors. (nih.gov)
• We report herein a CCR5-selective RNA aptamer that acts to facilitate entry through a simplified BBB model and that drives the uptake of LNPs into CCR5-expressing cells, while the gp160 aptamer did not. (beilstein-journals.org)
• Red visualizes the uptake of cell-penetrating peptides by green fluid-filled vesicles of the endosome (gray), a cell organelle involved in molecular transport. (genengnews.com)
• Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway. (nih.gov)
• The team also confirmed the properties of these peptides, including better uptake and binding within cells as well as no indication of cell death. (nih.gov)

Proteins4

• The cargoes they can deliver range from other small peptides, full-length proteins, nucleic acids including RNA and DNA, liposomes, nanoparticles, and viral particles as well as radioisotopes and other fluorescent probes for imaging purposes. (mdpi.com)
• Wilmer Eye Institute, Johns Hopkins Medicine researchers say they have used artificial intelligence models and machine-learning algorithms to successfully predict which components of amino acids that make up therapeutic proteins are most likely to safely deliver therapeutic drugs to animal eye cells. (nih.gov)
• The new research showed that artificial intelligence-designed models accurately predicted an effective sequence of amino acids, also known as peptides or small proteins, that would bind to a particular chemical in rabbit eye cells and safely dispense medications over several weeks, reducing the need for frequent, strict treatment schedules. (nih.gov)
• But what this colorful concoction lacks in taste, it makes up for as a valuable screening tool for peptides, miniature versions of proteins that our bodies use to control many cellular processes. (nih.gov)

CPPs2

• Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs), first identified ~25 years ago, are small, 6-30 amino acid long, synthetic, or naturally occurring peptides, able to carry variety of cargoes across the cellular membranes in an intact, functional form. (mdpi.com)
• Cell-penetrating peptides (CPPs) appear as a possibility to increase the cellular delivery of non-permeant biomolecules such as nucleic acids. (ox.ac.uk)

Nucleic acids6

• Thyclotides, tetrahydrofuran-modified peptide nucleic acids that efficiently penetrate cells and inhibit microRNA-21. (nih.gov)
• Cyclopentane peptide nucleic acid: Gold nanoparticle conjugates for the detection of nucleic acids in a microfluidic format. (nih.gov)
• Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA. (nih.gov)
• PPG peptide nucleic acids that promote DNA guanine quadruplexes. (nih.gov)
• Cyclopentane-peptide nucleic acids for qualitative, quantitative, and repetitive detection of nucleic acids. (nih.gov)
• We are developing new types of peptide nucleic acids (PNAs) for use in detection of nucleic acids as well as for multivalent display at the nanometer scale. (nih.gov)

Oligonucleotides2

• Delivery of therapeutic oligonucleotides with cell penetrating peptides. (ox.ac.uk)
• Recent data on mechanisms and applications of transfection strategies by cell penetrating peptides (CPP) are summarized on the variety of different oligonucleotide cargoes including plasmid, antisense and siRNA oligonucleotides. (pharmaceuticsconference.com)

Ability to penetrate1

• The artificial intelligence model generated 127 peptides that were predicted to have varying ability to penetrate the specialized cells that house melanin, to bind to melanin and to be nontoxic to the cells. (nih.gov)

Plasma membrane directly1

• This arginine-rich TAT peptide penetrates plasma membrane directly, but not through endocytosis. (anaspec.com)

Vivo7

• Introduction of PepFect and NickFect technologies for oligonucleotide transfection by transportan-based cellpenetrating peptides in vitro and in vivo is described. (pharmaceuticsconference.com)
• 2016) Recent in vivo advances in cell-penetrating peptide-assisted drug delivery. (pharmaceuticsconference.com)
• Previously, we developed a cell-penetrating near-infrared fluorescence (NIRF) probe based on an activatable strategy to detect apoptosis-associated caspase activity in vivo. (mcw.edu)
• Activating Transcription Factor 5 (ATF5) is highly expressed in gliomas, and interference with its expression/function precipitates targeted glioma cell apoptosis in vitro and in vivo. (nih.gov)
• In vivo, subcutaneously-administered Pen-d/n-ATF5-RP passed the blood brain barrier, entered normal brain and tumor cells, and then caused rapid selective tumor cell death. (nih.gov)
• The C. elegans developmental fusogenEFF-1 mediates homotypic fusion in heterologous cells and in vivo. (nih.gov)
• Using ligands specific for an integrin receptor, we have shown that the optimized γ-Lys-PNA multivalent scaffold improves biological activity of the ligand by two orders of magnitude in both cell-based and in vivo models of cancer metastasis. (nih.gov)

Membrane4

• G protein-coupled receptors (GPCRs) initiate signaling cascades while ensconced in the cell membrane. (genengnews.com)
• Given the closer proximity between endosomes and the nucleus, compared with the plasma membrane and nucleus, perhaps cells take advantage of the 3D spatial organization of cellular organelles and use a 'short cut' for efficient receptor signal transduction. (genengnews.com)
• He can also see which structures remain stable in the bloodstream and aren't readily degraded by proteases, enzymes that break down unwelcome peptides, and which ones don't toxically damage the cell membrane. (nih.gov)
• Cell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking. (nih.gov)

Vitro1

• In vitro Pen-d/n-ATF5-RP entered into glioma cells and triggered massive apoptosis. (nih.gov)

Fluorescent3

• An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging. (mcw.edu)
• This is a fluorescent (TAMRA)-labeled TAT peptide, Abs/Em=541/568 nm. (anaspec.com)
• Using cell cytometry (FACS) and fluorimetry, we demonstrated in yeast the successful expression and translational regulation of a fusion of mRNA binding protein and fluorescent protein. (igem.org)

Cargoes1

• They're synthesized in the lab, allowing Wimley and team to tweak their chemical structures and hopefully create ones with therapeutic potential, particularly as smart-delivery systems to target cells with greater precision and deliver biological cargoes such as drugs [1]. (nih.gov)

Synthetic2

• The synthetic, cell penetrating hexapeptide PAF26 (RKKWFW) is antifungal at low micromolar concentrations and has been proposed as a model for cationic, cell -penetrating antifungal peptides . (bvsalud.org)
• 1] Synthetic molecular evolution of hybrid cell penetrating peptides . (nih.gov)

Cellular2

• P53 is a tumour suppressor protein that regulates the expression of a wide variety of genes involved in Apoptosis, Growth arrest, Inhibition of cell cycle progression, Differentiation and accelerated DNA repair or Senescence in response to Genotoxic or Cellular Stress. (cupidpeptides.com)
• Mammalian cell biology is dynamic, but today's 'omic approaches require destruction of cells to access their molecular contents and cannot resolve the full richness of cellular diversity in situ . (nih.gov)

Intracellular1

• Efficient entry of cell-penetrating peptide nona-arginine into adherent cells involves a transient increase in intracellular calcium. (nih.gov)

Therapeutics2

• Lim studies the development of novel cancer therapeutics (gene therapies, protein/peptide therapies). (utah.edu)
• Computational Modeling of Stapled Peptides toward a Treatment Strategy for CML and Broader Implications in the Design of Lengthy Peptide Therapeutics. (utah.edu)

Selective1

• Selective inhibition of endosomal β2AR and Gαs signaling blunted nuclear ERK activity, MYC gene expression, and cell proliferation. (genengnews.com)

Sequence2

• This probe consisted of a cell-penetrating Tat peptide conjugated to an effector recognition sequence (DEVD) that was flanked by a fluorophore-quencher pair (Alexa Fluor 647 and QSY 21). (mcw.edu)
• Herein, we present biochemical analysis of a second generation probe, KcapQ, with a modified cell-penetrating peptide sequence (KKKRKV). (mcw.edu)

Receptor3

• In this review, we will first discuss the historical development of various NVCVs and then will discuss functionalized NVCVs with linkers that provide stability, as well as respond to the cancer cell environment and with cancer cell receptor specific ligands to explicitly target them for improved siRNA efficacy. (nih.gov)
• The first was centered on the BBB-penetrating trans-activator of transcription (Tat) peptide or the peptide T7, and the other on RNA aptamers targeted to glycoprotein gp160 from human immunodeficiency virus (HIV) or C-C chemokine receptor type 5 (CCR5), a HIV-1 coreceptor. (beilstein-journals.org)
• Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds. (nih.gov)

Therapies1

• Wimley is working hard to change that, since peptides have shown promise as therapies against some microbes, dubbed "superbugs," that have developed resistance to multiple drugs and present a serious health threat, especially to people with weakened immune systems. (nih.gov)

Gene3

• This interaction causes p53 repression which, through differential target gene expression, determines cell fate is of major interest to Cancer therapy. (cupidpeptides.com)
• Initially, we developed the technology to monitor changes in gene expression (genomics) in single cells. (nih.gov)
• This allows us to monitor the activation of different transcription factors and the array of genes each one regulates to investigate how different signaling pathways interact in controlling gene expression in a single cell. (nih.gov)

Nanoparticles1

• Lipid nanoparticles (LNPs) constitute a facile and scalable approach for delivery of payloads to human cells. (beilstein-journals.org)

Antioxidant1

• Paired with the ultra-potent antioxidant, this serum is packed with peptides and lactic acid that encourage and promote a faster cell-turnover and a more refined skin texture. (skinstore.com)

Near-infrared3

• To overcome the limitations of traditional near-infrared imaging probes, researchers from the University of Illinois at Chicago have designed a new probe specifically designed to detect cancerous cells within breast tumor margins in real time during surgery. (nih.gov)
• Our new probe, termed ICG-p28, can selectively illuminate breast cancer cells under near-infrared light and thereby facilitates the image-guided removal of cancerous tissue at the time of surgery. (nih.gov)
• To do this, they created a breast cancer cell line that expresses a protein called iRFP, which fluoresces under near-infrared light. (nih.gov)

Regulatory1

• Protection of the mucus layer involves mucins and a number of regulatory peptides, such as intestinal trefoil factors (ITFs), which play critical rols in formation and stabilization of the barrier, enhancement of rapid intestinal repair ('restitution), modulation of mucosal differentiation processes, and modulation of the mucosal immune response. (nih.gov)

Advances1

• Basics and recent advances in peptide and protein drug delivery. (utah.edu)

Dominant-negative2

• Regression/eradication of gliomas in mice by a systemically-deliverable ATF5 dominant-negative peptide. (nih.gov)
• Mitochondrially targeted p53 or DBD subdomain is superior to wild type p53 in ovarian cancer cells even with strong dominant negative mutant p53. (utah.edu)

Biol4

• 2007) Mol Biol Cell 18, 1153-66. (nih.gov)
• 2013) J Cell Biol 200:109-23. (nih.gov)
• Nat Rev Mol Cell Biol. (cupidpeptides.com)
• Harms KL, Chen X. Mol Cell Biol. (cupidpeptides.com)

Pathways1

• 4) Neural precursor cells possess multiple p53-dependent apoptotic pathways . (cupidpeptides.com)

Molecular1

• We have developed a novel nanopipette technology that can be used to monitor the molecular properties of single cells over time. (nih.gov)

Apoptosis3

• 6). Analysis of apoptosis in HeLa cells treated with doxorubicin showed probe activation specific to apoptotic cells. (mcw.edu)
• In a rat model of retinal neuronal excitotoxicity, intravitreal injection of N-methyl-d-aspartate (NMDA) induced apoptosis of retinal ganglion cells (RGCs). (mcw.edu)
• Thus, KcapQ represents an improved effector caspase-activatable NIRF probe for enhanced noninvasive analysis of apoptosis in whole cells and live animals. (mcw.edu)

Transcription2

• The enzyme called extracellular-signal-regulated kinase (ERK) regulates transcription of a number of genes involved in controlling cell growth. (genengnews.com)
• Now, in addition to single-cell sequencing technology, we have integrated methods to monitor temporal changes in the phosphorylation and activation of transcription factors. (nih.gov)

Tissues1

• The research community needs tools that can probe living cells in real tissues to produce high dimensional time-series data from cells of interest. (nih.gov)

Interaction3

• Two functional motifs define the interaction, internalization and toxicity of the cell-penetrating antifungal peptide PAF26 on fungal cells. (bvsalud.org)
• Significantly, cationic residues in PAF26 are important not only for the electrostatic attraction and interaction with the fungal cell but also for transport from the vacuole to the cytoplasm , which coincides with cell death . (bvsalud.org)
• Electroporation and electrophoretic DNA transfer into cells: the effect of DNA interaction with electropores. (nih.gov)

Tumor3

• However, around 20 percent of these surgeries require a second operation, generally because cancer cells are found within the tumor margins. (nih.gov)
• Now, NIBIB-funded researchers are developing an imaging method that would allow surgeons to better identify cancerous cells in the tumor margins during surgery. (nih.gov)
• This probe combines the FDA-approved dye ICG with a non-toxic, tumor-targeting, cell-penetrating peptide known as p28. (nih.gov)

Signaling2

• Alunni A, Krecsmarik M, Bosco A, Galant S, Pan L, Moens CB, Bally-Cuif L. Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium. (gene-tools.com)
• We found that GPCR-mediated ERK signaling, upon activation at the endosomes, propagates into the nucleus, where it turns on important genes to control cell growth," Zhang explained. (genengnews.com)

Cytokines1

• When the cell is confronted with stress through DNA damage, Hypoxia, Cytokines, Metabolic changes, Viral infection or Oncogenes, however, p53 ubiquitylation is suppressed and p53 accumulates in the nucleus, where it is activated and stabilized by undergoing multiple covalent modifications including Phosphorylation and Acetylation (1-4). (cupidpeptides.com)

Inhibition2

• Inhibition of bcr-abl in human leukemic cells with a coiled-coil protein delivered by a leukemia-specific cell-penetrating Peptide. (utah.edu)
• A chimeric p53 evades mutant p53 transdominant inhibition in cancer cells. (utah.edu)

Nucleus1

• Using the soup, Wimley can choose the rarer peptides (labeled with a unique color to make them stand out) that are able to cross membranes to gain access to the watery cytoplasm, as well as look for those that target specific compartments inside the cell, such as the nucleus. (nih.gov)

Microbes1

• Mutualistic microbes, comprising over 10 trillion microbial cells, inhabit the human gastrointestinal (GI) tract and generate functional metabolites that regulate immunity and host metabolism ( O'Hara and Shanahan, 2006 ). (frontiersin.org)

Methods2

• The winners will share $400,000 in prizes awarded for the development of new tools and methods for predicting the behavior and function of a single cell in complex tissue - and how that reflects the health of the tissue. (nih.gov)
• Phase 1 of the Follow that Cell Challenge sought novel robust methods for analysis of individual cells to detect and assess changes in cell behavior and function over time either as a result of natural state changes or when perturbed. (nih.gov)

HeLa1

• Specific inhibitory ligands of SCARAs, such as fucoidin, polyinosinic acid, and dextran sulfate, totally inhibit the activity of PF14-oligonucleotide nanocomplexes in the HeLa pLuc705 splice-correction cell model, while nonspecific, chemically related molecules do not. (ox.ac.uk)

Moisture1

• This powerful ingredient comes together with peptide-rich polyglutamic acid to prevent future moisture loss and a blend of plum and ice Willowherb, which rebalance the skin's natural oils while infusing it with antioxidants and other microbiome-strengthening nutrients. (travelandleisure.com)

Model2

• Out of these 127 peptides, the model predicted that a peptide called HR97 had the highest success rate of binding. (nih.gov)
• To address this problem the authors recently developed a neprilysin containing a brain-transport peptide that was able to reduce CNS Aβ and improve learning and memory in an APP Tg mouse model of AD. (nih.gov)

Small1

• Small rice protein peptides penetrate to stimulate cell growth and collagen formation, resulting in more skin elasticity. (eltamd.com)

Previously1

• Our research group has previously reported that p28 preferentially enters into various human breast cancer cells and actually induces anti-proliferative effects," explained Tohru Yamada, Ph.D., an associate professor in the Department of Surgery at the University of Illinois College of Medicine and senior author of the study. (nih.gov)

Regulates2

• Abu-Remaileh M, Gerson A, Farago M, Nathan G, Alkalay I, Zins Rousso S, Gur M, Fainsod A, Bergman Y. Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling. (gene-tools.com)
• Cell-surface phosphatidylserine regulates osteoclast precursor fusion. (nih.gov)

Transport1

• Live- cell imaging of GFP-labelled nuclei in A. fumigatus showed that transport of PAF26 from the vacuole to the cytoplasm was followed by nuclear breakdown and dissolution . (bvsalud.org)

Species1

• These dsRNA-mediated processes are evolutionarily conserved and are thought to be involved in transposon silencing in various species, although evidence of a natural role in the majority of human cells is still scant. (nih.gov)

Types2

• In an effort to target LNPs composed of an ionizable cationic lipid (DLin-MC3-DMA), cholesterol, the phospholipid 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), and 1,2-dimyristoyl- rac -glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG 2000) to particular cell types, as well as to generate LNPs that can cross the BBB, we developed and assessed two approaches. (beilstein-journals.org)
• Wimley can then study the peptides further to see which types of cargo they can deliver to cells. (nih.gov)

Recombinant1

• The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes. (nih.gov)

Cancer6

• 9. The investigation of unique water-soluble heptamethine cyanine dye for use as NIR photosensitizer in photodynamic therapy of cancer cells. (nih.gov)
• Scanning electron microscopy image of a breast cancer cell. (nih.gov)
• This way, when the researchers establish their breast cancer models by injecting mice with this particular cell line, they can precisely visualize the exact shape and size of the breast tumors. (nih.gov)
• Carol Lim, PhD, is Interim Chair and Professor in the Departments of Pharmaceutics and Pharmaceutical Chemistry at the University of Utah and a member of the Cell Response and Regulation Program at Huntsman Cancer Institute. (utah.edu)
• To establish the utility of this multiplexed technology, we are employing it to study a critical biological question: How do cancer cells develop drug resistance? (nih.gov)
• While the proposed studies focus on the use of our nanopipette technology to study human cancer cells in culture, the cell monitoring capabilities of the nanopipette allow it to be used for in situ analysis of single cells in tissue biopsies and slices. (nih.gov)