Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A mercaptoethylamine compound that is endogenously derived from the COENZYME A degradative pathway. The fact that cysteamine is readily transported into LYSOSOMES where it reacts with CYSTINE to form cysteine-cysteamine disulfide and CYSTEINE has led to its use in CYSTINE DEPLETING AGENTS for the treatment of CYSTINOSIS.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Venoms produced by the wasp (Vespid) family of stinging insects, including hornets; the venoms contain enzymes, biogenic amines, histamine releasing factors, kinins, toxic polypeptides, etc., and are similar to bee venoms.
A neuropeptide of 29-30 amino acids depending on the species. Galanin is widely distributed throughout the BRAIN; SPINAL CORD; and INTESTINES. There are various subtypes of GALANIN RECEPTORS implicating roles of galanin in regulating FOOD INTAKE; pain perception; memory; and other neuroendocrine functions.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Transport proteins that carry specific substances in the blood or across cell membranes.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).
Dental procedure in which part of the pulp chamber is removed from the crown of a tooth.
The barrier between capillary blood and alveolar air comprising the alveolar EPITHELIUM and capillary ENDOTHELIUM with their adherent BASEMENT MEMBRANE and EPITHELIAL CELL cytoplasm. PULMONARY GAS EXCHANGE occurs across this membrane.
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
A pancreatic beta-cell hormone that is co-secreted with INSULIN. It displays an anorectic effect on nutrient metabolism by inhibiting gastric acid secretion, gastric emptying and postprandial GLUCAGON secretion. Islet amyloid polypeptide can fold into AMYLOID FIBRILS that have been found as a major constituent of pancreatic AMYLOID DEPOSITS.
A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. Activated Smad3 can bind directly to DNA, and it regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.
A signal transducing adaptor protein and tumor suppressor protein. It forms a complex with activated RECEPTOR-REGULATED SMAD PROTEINS. The complex then translocates to the CELL NUCLEUS and regulates GENETIC TRANSCRIPTION of target GENES.
A family of proteins that are involved in the translocation of signals from TGF-BETA RECEPTORS; BONE MORPHOGENETIC PROTEIN RECEPTORS; and other surface receptors to the CELL NUCLEUS. They were originally identified as a class of proteins that are related to the mothers against decapentaplegic protein, Drosophila and sma proteins from CAENORHABDITIS ELEGANS.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by BONE MORPHOGENETIC PROTEIN RECEPTORS. It regulates BONE MORPHOGENETIC PROTEIN signaling and plays an essential role in EMBRYONIC DEVELOPMENT.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
Phenomenon of cell-mediated immunity measured by in vitro inhibition of the migration or phagocytosis of antigen-stimulated LEUKOCYTES or MACROPHAGES. Specific CELL MIGRATION ASSAYS have been developed to estimate levels of migration inhibitory factors, immune reactivity against tumor-associated antigens, and immunosuppressive effects of infectious microorganisms.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.
Substances that are toxic to blood in general, including the clotting mechanism; hematotoxins may refer to the hematopoietic system.
Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Organic compounds that contain phosphorus as an integral part of the molecule. Included under this heading is broad array of synthetic compounds that are used as PESTICIDES and DRUGS.
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

Passage of cell-penetrating peptides across a human epithelial cell layer in vitro. (1/188)

Cell barriers are essential for the maintenance and regulation of the microenvironments of the human body. Cell-penetrating peptides have simplified the delivery of bioactive cargoes across the plasma membrane. Here, the passage of three cell-penetrating peptides (transportan, the transportan analogue transportan 10, and penetratin) across a Caco-2 human colon cancer cell layer in vitro was investigated. The peptides were internalized into epithelial Caco-2 cells as visualized by indirect fluorescence microscopy and quantified by fluorimetry. Studies of peptide outflow from cells showed that the peptides were in equilibrium across the plasma membrane. The ability of the peptides to cross a Caco-2 cell layer was tested in a two-chambered model system. After 120 min, 7.0%, 2.8% and 0.6% of added transportan, transportan 10 and penetratin respectively was detected in the lower chamber. Both transportan and transportan 10 reversibly decreased the trans-epithelial electrical resistance of the barrier model, with minimum values after 60 min of 46% and 60% of control respectively. Penetratin did not affect the resistance of the cell layer to the same extent. Although transportan markedly increased the passage of ions, the paracellular flux of 4.4 kDa fluorescein-labelled dextran was limited. In conclusion, the results indicate that the transportan peptides pass the epithelial cell layer mainly by a mechanism involving a transcellular pathway.  (+info)

TAT peptide and its conjugates: proteolytic stability. (2/188)

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MALDI-TOF mass spectrometry: a powerful tool to study the internalization of cell-penetrating peptides. (3/188)

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Lipid domain separation, bilayer thickening and pearling induced by the cell penetrating peptide penetratin. (4/188)

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Cell-penetrating peptide exploited syndecans. (5/188)

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Cell biology meets biophysics to unveil the different mechanisms of penetratin internalization in cells. (6/188)

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Peptide-mediated protein delivery-which pathways are penetrable? (7/188)

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Morpholinos and their peptide conjugates: therapeutic promise and challenge for Duchenne muscular dystrophy. (8/188)

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Identification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a cancer-homing transporterIdentification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a cancer-homing transporterAA11542044 ...
The ability of cell-penetrating peptides to cross plasma membranes has been explored for various applications, including the delivery of bioactive molecules to inhibit disease-causing cellular processes. The uptake mechanisms by which cell-penetrating peptides enter cells depend on the conditions, such as the cell line the concentration and the temperature. To be used as therapeutics, each novel cell-penetrating peptide needs to be fully characterized, including their physicochemical properties, their biological activity and their uptake mechanism. Our group has developed a series of highly performing, non-toxic cell-penetrating peptides, all derived from the original sequence of transportan 10. These analogs are called PepFects and NickFects and they are now a diverse family of N-terminally stearylated peptides. These peptides are known to form noncovalent, nano-sized complexes with diverse oligonucleotide cargoes. One bottleneck that limits the use of this technology for gene therapy ...
Cell-penetrating peptides (CPPs) are prominent delivery vehicles to confer cellular entry of (bio-) macromolecules. Internalization efficiency and uptake mechanism depend, next to the type of CPP and cargo, also on cell type. Direct penetration of the plasma membrane is the preferred route of entry as this circumvents endolysosomal sequestration. However, the molecular parameters underlying this import mechanism are still poorly defined. Here, we make use of the frequently used HeLa and HEK cell lines to address the role of lipid composition and membrane potential. In HeLa cells, at low concentrations, the CPP nona-arginine (R9) enters cells by endocytosis. Direct membrane penetration occurs only at high peptide concentrations through a mechanism involving activation of sphingomyelinase which converts sphingomyelin into ceramide. In HEK cells, by comparison, R9 enters the cytoplasm through direct membrane permeation already at low concentrations. This direct permeation is strongly reduced at ...
article{d17966db-786f-4d12-a5f5-3a25a837ce80, abstract = {,p,Small-angle X-ray scattering (SAXS) measurements reveal a striking difference in intermolecular interactions between two short highly charged peptides - deca-arginine (R10) and deca-lysine (K10). Comparison of SAXS curves at high and low salt concentration shows that R10 self-associates, while interactions between K10 chains are purely repulsive. The self-association of R10 is stronger at lower ionic strengths, indicating that the attraction between R10 molecules has an important electrostatic component. SAXS data are complemented by NMR measurements and potentials of mean force between the peptides, calculated by means of umbrella-sampling molecular dynamics (MD) simulations. All-atom MD simulations elucidate the origin of the R10- R10 attraction by providing structural information on the dimeric state. The last two C-terminal residues of R10 constitute an adhesive patch formed by stacking of the side chains of two arginine residues ...
Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification, Scientific Reports 6, 36938 (2016). Since their discovery twenty years ago, cell-penetrating peptides (CPPs) or protein transduction domains (PTDs) have been described as promising drug delivery systems. There are increasing numbers of successful applications of CPPs/PTDs in vivo. However, one of the limitations to their wide and diverse application is the diversity of their uptake pathways. The CPP and its cargo may end up free in the cytosol and reach their biological target only following translocation, but remain confined in intracellular vesicles after endocytosis, unless subsequent events such as endosomal rupture occur. Along with the development of CPPs/PTDs as vectors to carry various macromolecules for targeted cellular therapies, engineering new CPPs/PTDs with optimized transport and ...
Arginine-rich cell-penetrating peptides (CPP) are widely employed as delivery vehicles for a large variety of macromolecular cargos. As a mechanism-of-action for induction of uptake cross-linking of heparan sulfates and interaction with lipid head groups have been proposed. Here, we employed a multivalent display of the CPP nona-arginine (R9) on a linear dextran scaffold to assess the impact of heparan sulfate and lipid interactions on uptake and membrane perturbation. Increased avidity through multivalency should potentiate molecular phenomena that may only play a minor role if only individual peptides are used. To this point, the impact of multivalency has only been explored for dendrimers, CPP-decorated proteins and nanoparticles. We reasoned that multivalency on a linear scaffold would more faithfully mimic the arrangement of peptides at the membrane at high local peptide concentrations. On average, five R9 were coupled to a linear dextran backbone. The conjugate displayed a direct ...
TY - JOUR. T1 - Matrix metalloproteinase-1 induces cleavage of exogenous alphab-crystallin transduced by a cell-penetrating peptide. AU - Yang, Seung Won. AU - Lee, Seung Min. AU - Choi, Eun Young. AU - Lee, Kyung Hye. AU - Kim, Soo Hyuk. AU - Shin, Min Jeong. AU - Han, Ye Sun. AU - Kang, Seok Min. AU - Chung, Ji Hyung. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Cell-penetrating peptides (CPPs), including TAT-CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT-CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT-fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB-crystallin (αB-crystallin), delivered by TAT-CPP is susceptible to proteolytic cleavage by matrix metalloproteinase-1 (MMP-1) in cardiac myoblast H9c2 cells. Recombinant TAT-αB-crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of ...
Genes are the major regulators of biological processes in every living thing. Problems with gene regulation can cause serious problems for the organism; for example, most cancers have some kind of genetic component. Regulation of biological processes using oligonucleotides can potentially be a therapy for any ailment, not just cancer. The problem so far has been that the targets for oligonucleotide-based therapies all reside on the inside of cells, because the cellular plasma membrane is normally impermeable to large and charged molecules (such as oligonucleotides) a delivery method is needed. Cell-penetrating peptides are a class of carrier molecules that are able to induce the cellular membrane into taking them and their cargo molecules into the cells. Understanding how and why cell-penetrating peptides work is one of the first and most important steps towards improving them to the point where they become useful as carriers for oligonucleotide-based therapies. This thesis is comprised of four ...
Regulation of biological processes through the use of genetic elements is a central part of biological research and also holds great promise for future therapeutic applications. Oligonucleotides comprise a class of versatile biomolecules capable of modulating gene regulation. Gene therapy, the concept of introducing genetic elements in order to treat disease, presents a promising therapeutic strategy based on such macromolecular agents. Applications involving charged macromolecules such as nucleic acids require the development of the active pharmaceutical ingredient as well as efficient means of intracellular delivery. Cell-penetrating peptides are a promising class of drug delivery vehicles, capable of translocation across the cell membrane together with molecules otherwise unable to permeate cells, which has gained significant attention. In order to increase the effectiveness of cell-penetrating peptide-mediated delivery, further understanding of the mechanisms of uptake is needed in addition ...
Redesigning linear cell penetrating peptides (CPPs) into a multi-branched topology with short dipeptide branches gave cell penetrating peptide dendrimers (CPPDs) with higher cell penetration, lower toxicity and hemolysis and higher serum stability than linear CPPs. Their use is demonstrated by delivering a c
The tumor suppressor p14ARF is widely deregulated in many types of cancers and is believed to function as a failsafe mechanism, inhibiting proliferation and inducing apoptosis as cellular response to a high oncogene load. We have found that a 22-amino-acid-long peptide derived from the N-terminal part of p14ARF, denoted ARF(1-22), which has previously been shown to mimic the function of p14ARF, has cell-penetrating properties. This peptide is internalized to the same extent as the cell-penetrating peptide (CPP) TP10 and dose-dependently decreases proliferation in MCF-7 and MDA MB 231 cells. Uptake of the ARF(1-22) peptide is associated with low membrane disturbance, measured by deoxyglucose and lactate dehydrogenase (LDH) leakage, as compared to its scrambled peptide. Also, flow cytometric analysis of annexin V/propidium iodide (PI) binding and Hoechst staining of nuclei suggest that ARF(1-22) induces apoptosis, whereas scrambled or inverted peptide sequences have no effect. The ARF(1-22) peptide mainly
The use of CPPs (cell-penetrating peptides) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. Since then, several CPPs, including the widely used Tat (transactivator of transcription) peptide, have been developed and utilized to translocate a wide range of compounds across the plasma membrane of cells both in vivo and in vitro. Although the field has emerged as a possible future candidate for drug delivery, little attention has been given to the potential toxic side effects that these peptides might exhibit in cargo delivery. Also, no comprehensive study has been performed to evaluate the relative efficacy of single CPPs to convey different cargos. Therefore we selected three of the major CPPs, penetratin, Tat and transportan 10, and evaluated their ability to deliver commonly used cargos, including fluoresceinyl moiety, double-stranded DNA and proteins (i.e. avidin and streptavidin), and studied their effect on membrane
CPPs (cell-penetrating peptides) facilitate cellular uptake of covalently attached macromolecules, through an as yet controversial mechanism that either involves direct membrane passage or a type of endocytosis. We investigated the potential of the CPPs penetratin and Tat to act as mitochondria-targeting vectors by testing whether they were internalized by isolated mitochondria, and by mitochondria within cells in culture. We also tested peptides conjugated to the mitochondria-targeting moiety triphenylphosphonium. We found no evidence for mitochondrial uptake by penetratin, Tat or their triphenylphosphonium conjugates. This result suggests that CPPs are unsuitable as mitochondria-targeting vectors, and implies an endocytic mode of cellular uptake for CPPs. ...
TY - JOUR. T1 - Better living through peptide-conjugated chemistry. T2 - Next-generation antisense oligonucleotides. AU - McNally, Elizabeth M.. AU - Leverson, Brian D.. PY - 2019/11/1. Y1 - 2019/11/1. N2 - Two different antisense oligonucleotide-based (ASO-based) therapies are currently in clinical use to treat neuromuscular diseases. This success, for Duchenne muscular dystrophy and spinal muscular atrophy, offers hope not only for additional neuromuscular diseases, but also for other disorders that could benefit from RNA-targeted therapies. A major limitation for more widespread application of ASOs relates to relatively poor tissue penetration. In this issue of the JCI, Klein et al. showed that conjugating an ASO with an arginine-rich cell-penetrating peptide, Pip6a, enhanced delivery, resulting in corrective outcome for a mouse model of myotonic dystrophy. Linking ASOs to cell-penetrating peptides, or even other moieties, is an approach currently under development with treatment potential to ...
Cell-penetrating peptides (CPP) have attracted many scientists attention as intracellular delivery tools due to their high cargo molecule transportation efficiency and low cytotoxicity. Therefore, in many research fields CPP, such as HIV-Tat and oligoarginine (Rn), are used to deliver hydrophilic drugs and biomolecules, including proteins, DNA, and RNA. We designed four types of CPP that contained cationic α,α-disubstituted amino acids (Api(C2Gu) and Api(C4Gu)) as helical promoters; i.e., 1-4 [FAM-β-Ala-(l-Arg-l-Arg-Xaa)3-(Gly)3-NH2 (1: Xaa=Api(C2Gu), 2: Xaa=Api(C4Gu)), 3: FAM-β-Ala-(l-Arg)8-Api(C2Gu)-(Gly)3-NH2, and 4: FAM-β-Ala-(l-Arg)5-Api(C2Gu)-(l-Arg)2-Api(C2Gu)-(Gly)3-NH2], and investigated their preferred secondary structures and cell membrane-penetrating ability. As a result, we found that the permeation efficiency of the CPP was affected by the number of helical promoters in their sequences. Specially, peptide 1, which contained three Api(C2Gu) residues, formed a stable helical ...
DUGi: Viewing Item from repository Recercat: This thesis is focused on the development of synthetic approaches to obtain new bioactive peptides. The first part deals with the design of new antimicrobial peptide/cell-penetrating peptide conjugates as anticancer agents. Their conjugation enhanced the activity of the antimicrobial peptides against cancer cells while maintained their low toxicity. These compounds are interesting for the design of new anticancer agents. On the second part, a new versatile methodology for the synthesis of natural fengycin derivatives is described. Our strategy represents the first synthetic approach for the total solid-phase synthesis of these cyclic lipodepsipeptides and can be easily adapted to obtain a wide range of analogues.
|p|Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. AMPs are in various stages of drug development, from early preclinical studies to phase III of clinical studies . Compounds with different molecular sizes were examined, from the peptide containing 24 amino acids, OP-145, to the modified tripeptide LTX-109 , both in phase II of clinical trial. Trojan peptides or cell-penetrating peptides (CPP) are natural or designed peptides identified as cellular membrane-crossing molecules, in particular through their potency to vehiculate various kinds of compounds to the cytoplasm and nucleus of living cells.|/p|
DUGi: Viewing Item from repository DUGiDocs: Peptide conjugates incorporating the red-ox active ligands Me2PyTACN or (S,S)-BPBP at the N- or the C-terminus of the cell-penetrating peptide BP16 were synthesized (PyTACN-BP16 (BP341), BP16-PyTACN (BP342), BPBP-BP16 (BP343), and BP16-BPBP (BP344)). Metal binding peptides bearing at the N-terminus the ligand, an additional Lys and a β-Ala were also prepared (PyTACN-βAK-BP16 (BP345) and BPBP-βAK-BP16 (BP346)). Moreover, taking into account the clathrin-dependent endocytic mechanism of BP16, the enzymatic cleavable tetrapeptide Gly-Phe-Leu-Gly was incorporated between the ligand and the N- or C-terminus of BP16 (BPBP-GFLG-BP16 (BP347) and BP16-GLFG-BPBP (BP348). Analysis of the cytotoxicity of all the peptide conjugates showed that: (i) the position of the ligand influenced the IC50 values, (ii) the incorporation of the βAla-Lys dipeptide rendered non active sequences, (iii) peptide conjugates derived from the (S,S)-BPBP ligand were more active than
Non-injectable delivery of peptides and proteins is not feasible due to the limitations of large molecular mass, high hydrophilic properties, and gastrointestinal degradation. Therefore, proposing a new method to solve this problem is a burning issue. The objective of this study was to propose a novel protein delivery strategy to overcome the poor efficacy and irritation of buccal insulin delivery. In this study, we applied a conjugate of cell-penetrating peptides (LMWP) and insulin (INS-PEG-LMWP) for buccal delivery. INS-PEG-LMWP was prepared using insulin solution and mixture as references. The transport behaviour, in vivo bioactivity, hypoglycaemic effect, and safety of INS-PEG-LMWP were systematically characterised. An in vitro study demonstrated that the uptake and transportation of INS-PEG-LMWP across buccal mucosal multilayers significantly increased. By comparing the effects of different endocytic inhibitors on INS-PEG-LMWP uptake, the conjugate might be delivered via an energy ...
Author: Jha, D et al.; Genre: Journal Article; Published in Print: 2011-03; Title: CyLoP-1: A Novel Cysteine-Rich Cell-Penetrating Peptide for Cytosolic Delivery of Cargoes
Read Progress in Research and Application of HIV-1 TAT-Derived Cell-Penetrating Peptide, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Author: Gottschalk, S et al.; Genre: Conference Paper; Published in Print: 2011-03; Title: Induction of Apoptosis by SmacN7 coupled to the Novel Cysteine-Rich Cell-Penetrating Peptide CyLoP-1
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Transposable elements have emerged as a promising candidate for human non-viral gene-therapy. The Tc1/mariner transposon Sleeping Beauty is to date one of the most efficient transposons in mammals. Sleeping Beauty transposase has so far mostly been delivered to cells via a DNA source. This might cause spontaneous integration of the transposase gene and cause fatal damage to the affected cell. Hence, it would be advantageous to employ a non-genetic source for the transposase. We here show that a novel Cell-penetrating peptide, M918, has the ability to facilitate cellular delivery of both the transposase Sleeping Beauty as a protein and a transposon donor-plasmid carrying an antibiotic resistance gene in vitro. The technique is a simple and straightforward one-step method that might render a safe and efficient delivery platform for Sleeping Beauty mediated gene therapy.. ...
Several recent studies have demonstrated that TAT and other arginine-rich cell penetrating peptides (CPPs) have intrinsic neuroprotective properties in their own right. Examples, we have demonstrated that in addition to TAT, poly-arginine peptides (R8 to R18; containing 8-18 arginine residues) as well as some other arginine-rich peptides are neuroprotective in vitro (in neurons exposed to glutamic acid excitotoxicity and oxygen glucose deprivation) and in the case of R9 in vivo (after permanent middle cerebral artery occlusion in the rat). Based on several lines of evidence, we propose that this neuroprotection is related to the peptides endocytosis-inducing properties, with peptide charge and arginine residues being critical factors. Specifically, we propose that during peptide endocytosis neuronal cell surface structures such as ion channels and transporters are internalised, thereby reducing calcium influx associated with excitotoxicity and other receptor-mediated neurodamaging signalling ...
As heparan sulfate proteoglycans (HSPGs) are known as co-receptors to interact with numerous growth factors and then modulate downstream biological activities, overexpression of HS/HSPG on cell surface acts as an increasingly reliable prognostic factor in tumor progression. Cell penetrating peptides (CPPs) are short-chain peptides developed as functionalized vectors for delivery approaches of impermeable agents. On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to multiple cellular effects. Here a functional peptide (CPP|i|ecp|/i|) has been identified from critical HS binding region in hRNase3, a unique RNase family member with|i| in vitro|/i| antitumor activity. In this study we analyze a set of HS-binding CPPs derived from natural proteins including CPP|i|ecp|/i|. In addition to cellular binding and internalization, CPP|i|ecp|/i| demonstrated multiple functions including strong binding activity to tumor cell surface with higher HS
We investigated the metabolic stability of four cell penetrating peptides (CPPs), namely SAP, hCT(9-32)-br, [Palpha] and [Pbeta], when in contact with either subconfluent HeLa, confluent MDCK or Calu-3 epithelial cell cultures. Additionally, through analysis of their cellular translocation efficiency, we evaluated possible relations between metabolic stability and translocation efficiency. Metabolic degradation kinetics and resulting metabolites were assessed using RP-HPLC and MALDI-TOF mass spectrometry. Translocation efficiencies were determined using fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM). Between HeLa, MDCK and Calu-3 we found the levels of proteolytic activities to be highly variable. However, for each peptide, the individual degradation patterns were quite similar. The metabolic stability of the investigated CPPs was in the order of CF-SAP = CF-hCT(9-32)-br , [Pbeta]-IAF , [Palpha] and we identified specific cleavage sites for each of the ...
Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates and enzyme inhibitors. Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation of non-covalent complexes. The latter is often preferred because of easy handling, uptake efficiency and auto-release of cargo into the live cell. In our studies complexes are formed with labeled or readily detectable cargoes for qualitative and quantitative estimation of their internalization. Properties and behavior of adhesion and suspension vertebrate cells as well as the protozoa Leishmania tarentolae are investigated with respect to proteolytic activity, uptake efficiency, intracellular localization and cytotoxicity. Our results show that peptide stability to membrane-bound, secreted or intracellular
Patients on BETH :. The LVEF monitoring sequence required by CADY will be altered to parallel that required by BETH. Thus, echocardiograph or MUGA will be carried out according to the BETH cardiac safety monitoring protocol. This altered schedule applies only to patients enrolled in both studies. Of note, for patients on the BETH study, all LVEF assessments must be performed by the same method used at baseline.. Patients on ALTTO:. It is possible that a patient who is enrolled on both CADY and ALLTO may not eventually receive Trastuzumab. Nonetheless, as Lapatinib is also associated with a risk of cardiotoxicity, such patients should remain on study whilst receiving single agent Lapatinib. The schedule of on study assessments will be identical to that of patients receiving Trastuzumab.. For patients enrolled in both studies, the LVEF monitoring sequence required by CADY will be altered to parallel that required by ALLTO. Thus, echocardiograph or MUGA will be carried out according to the ALLTO ...
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One oligonucleotide-based approach that appear very promising for the treatment of different genetic disorders are based on so-called splice-correcting oligonucleotides (SCOs) that are exploited to manipulate splicing patterns. In order to increase the bioavailability, cell-penetrating peptides (CPPs) have readily been covalently conjugated to SCOs to facilitate cellular internalization. While being a successful strategy for the delivery of uncharged oligonucleotides (ONs), it is extremely difficult to generate covalent conjugates between commonly used negatively charged ON analogs and cationic CPPs. Furthermore, high concentrations of ONs in the micromolar range are often needed to obtain biological responses, most likely as a result of endosomal entrapment of material. Therefore, exploring other vectorization methods using CPPs with endosomolytic properties are highly desired.A method of using stearyl modified CPP (i.e., TP10) analogs, named PepFect3 and PepFect4, are being described for the
Maurocalcine (MCa), initially identified from a Tunisian scorpion venom, defines a new member of the family of cell penetrating peptides by its ability to efficiently cross the plasma membrane. The initiating mechanistic step required for the cell translocation of a cell penetrating peptide implicates its binding onto cell surface components such as membrane lipids and/or heparan sulfate proteoglycans. Here we characterized the interaction of wild-type MCa and MCa K20A, a mutant analogue with reduced cell-penetration efficiency, with heparin (HP) and heparan sulfates (HS) through surface plasma resonance. HP and HS bind both to MCa, indicating that heparan sulfate proteoglycans may represent an important entry route of the peptide. This is confirmed by the fact that (i) both compounds bind with reduced affinity to MCa K20A and (ii) the cell penetration of wild-type or mutant MCa coupled to fluorescent streptavidin is reduced by about 50% in mutant Chinese hamster ovary cell lines lacking either all
Glycosaminoglycans (GAGs) contribute to the cellular uptake of cationic cell-penetrating peptides (CPPs). However, molecular details about the contributions of GAGs in CPP internalization remain unclear. In this study, we examined the cellular uptake mechanism of the arginine-rich CPP pituitary adenylate-cyclase-activating polypeptide (PACAP). We observed that the uptake efficacy of PACAP is dependent on the expression of cell surface GAGs. As the binding of PACAP to sulfated GAGs induced a random coil-to-α-helix conformational conversion, we investigated the role of the helical formation in PACAP internalization. Whereas this secondary structure was not crucial for efficient internalization in GAGs-deficient cells, PACAP α-helix was essential for GAGs-dependent uptake.. ...
Progress in our understanding of the molecular pathogenesis of human malignancies has provided therapeutic targets amenable to oligonucleotide (ON)-based strategies. Antisense ON-mediated splicing regulation in particular offers promising prospects since the majority of human genes undergo alternative splicing and since splicing defects have been found in many diseases. However, their implementation has been hampered so far by the poor bioavailability of nucleic acids-based drugs. Cell-penetrating peptides (CPPs) now appear as promising non-viral delivery vector for non-permeant biomolecules. We describe here new CPPs allowing the delivery of splice redirecting steric-block ON using either chemical conjugation or non-covalent complexation. We also describe a convenient and robust splice redirecting assay which allows the quantitative assessment of ON nuclear delivery.
A total of 2×105 HeLa or CHO cells were seeded 24 h before experiments in 12-well plates to reach 70% confluence. In the uptake studies on free peptide, cells were treated with 1 or 5 μM fluorescein-labelled peptides in 500 μl of serum-free medium. In transfection studies of dsDNA, peptides in various concentrations were co-incubated for 30 min in 50 μl of 0.9% NaCl solution. Cells were then treated with 0.2 μM fluorescein-labelled dsDNA and CPPs in 500 μl of serum-free medium. In the protein transduction experiments, streptavidin or avidin was either complexed with 1 μM biotinylated peptides in serum-free medium or co-incubated with free peptides at various molar ratios in 0.9% NaCl solution to a final volume of 50 μl. Cells were then treated with 0.2 μM protein and CPPs in 500 μl of serum-free medium. At 1 h after treatment or 90 min after treatment with proteins, cells were washed twice in HKR (Hepes-Krebs-Ringer) buffer before trypsinization. The trypsinization step is crucial in ...
Protein transduction domains (PTDs) are powerful nongenetic tools that allow intracellular delivery of conjugated cargoes to modify cell behavior. Their use in biomedicine has been hampered by inefficient delivery to nuclear and cytoplasmic targets. Here we overcame this deficiency by developing a series of novel fusion proteins that couple a membrane-docking peptide to heparan sulfate glycosaminoglycans (GAGs) with a PTD. We showed that this GET (GAG-binding enhanced transduction) system could deliver enzymes (Cre, neomycin phosphotransferase), transcription factors (NANOG, MYOD), antibodies, native proteins (cytochrome C), magnetic nanoparticles (MNPs), and nucleic acids [plasmid (p)DNA, modified (mod)RNA, and small inhibitory RNA] at efficiencies of up to two orders of magnitude higher than previously reported in cell types considered hard to transduce, such as mouse embryonic stem cells (mESCs), human ESCs (hESCs), and induced pluripotent stem cells (hiPSCs). This technology represents an ...
The increasing prevalence of multidrug-resistant bacteria urges the development of new antibacterial agents. With a broad spectrum activity, antimicrobial peptides have been considered potential antibacterial drug leads. Using bioinformatic tools we have previously shown that viral structural proteins are a rich source for new bioactive peptide sequences, namely antimicrobial and cell-penetrating peptides. Here, we test the efficacy and mechanism of action of the most promising peptides among those previously identified against both Gram-positive and Gram-negative bacteria. Two cell-penetrating peptides, vCPP 0769 and vCPP 2319, have high antibacterial activity against S. aureus, MRSA, E. coli and P. aeruginosa, being thus multifunctional. The antibacterial mechanism of action of the two most active viral protein-derived peptides, vAMP 059 and vCPP 2319, was studied in detail. Both peptides act on both Gram-positive S. aureus and Gram-negative P. aeruginosa, with bacterial cell death occurring within
TGF-β is considered a master switch in the pathogenesis of organ fibrosis. The primary mediators of this activity are the SMAD proteins, particularly SMAD3. In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an aminoterminal sequence of sorting nexin 9 (SNX9), which was previously shown to bind phosphorylated SMAD3 (pSMAD3). We determined that specifically preventing the nuclear import of pSMAD3 using the TAT-SNX9 peptide inhibited profibrotic TGF-β activity in murine cells and human lung fibroblasts as well as in vivo with no demonstrable toxicity. TGF-β signaling mediated by pSMAD2, bone morphogenetic protein 4 (BMP4), EGF, or PDGF was unaffected by the TAT-SNX9 peptide. Furthermore, while the TAT-SNX9 peptide prevented TGF-βs profibrotic activity in vitro as well as in 2 murine treatment models of pulmonary fibrosis, a 3-amino acid point mutant that was unable to bind pSMAD3 proved ineffective. These findings ...
TGF-β is considered a master switch in the pathogenesis of organ fibrosis. The primary mediators of this activity are the SMAD proteins, particularly SMAD3. In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an aminoterminal sequence of sorting nexin 9 (SNX9), which was previously shown to bind phosphorylated SMAD3 (pSMAD3). We determined that specifically preventing the nuclear import of pSMAD3 using the TAT-SNX9 peptide inhibited profibrotic TGF-β activity in murine cells and human lung fibroblasts as well as in vivo with no demonstrable toxicity. TGF-β signaling mediated by pSMAD2, bone morphogenetic protein 4 (BMP4), EGF, or PDGF was unaffected by the TAT-SNX9 peptide. Furthermore, while the TAT-SNX9 peptide prevented TGF-βs profibrotic activity in vitro as well as in 2 murine treatment models of pulmonary fibrosis, a 3-amino acid point mutant that was unable to bind pSMAD3 proved ineffective. These findings ...
Researchers suggest that the peptide constructs act as general amyloid inhibitors, with potential applications in many amyloid-related diseases.
Academic Journals Database is a universal index of periodical literature covering basic research from all fields of knowledge, and is particularly strong in medical research, humanities and social sciences. Full-text from most of the articles is available. Academic Journals Database contains complete bibliographic citations, precise indexing, and informative abstracts for papers from a wide range of periodicals.
With the advance of science and technology, nanomaterials have been seen in various biomedical applications, including molecular labeling and tracking, ...
Gene therapy is an approach that introduces nucleic acids into cells and aims to correct the gene function by altering the gene expression to prevent, halt, or reverse a pathological process (1). There are three main routes to gene therapy: restoring a lost gene function, silencing the disease-causing genes, or modifying a gene function. Achieving expression of a deficient gene product by therapeutic gene delivery is considered to be the classical gene therapy approach, but gene silencing and function modification have also gained quite an interest recently. RNA interference (RNAi) is a fundamental gene silencing pathway in eukaryotic cells, which is mediated by short interfering RNA (siRNAs) that can cleave complementary mRNA sequences with the help of the RNA-induced silenci ...
The presence of cell and tissue barriers together with the low biomembrane permeability of various therapeutics often hampers systemic drug distribution; thus, most of the available molecules are of limited therapeutic value. Opportunities to increase medicament concentrations in areas that are diff …
Identification of a cell-penetrating peptide domain from human beta-defensin 3 and characterization of its anti-inflammatory activity Jue Yeon Lee,1,* Jin Sook Suh,2,* Jung Min Kim,1 Jeong Hwa Kim,1 Hyun Jung Park,1 Yoon Jeong Park,1,2 Chong Pyoung Chung1 1Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), Chungcheongbuk-do, Republic of Korea; 2Dental Regenerative Biotechnology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea *These authors contributed equally to this work Abstract: Human beta-defensins (hBDs) are crucial factors of intrinsic immunity that function in the immunologic response to a variety of invading enveloped viruses, bacteria, and fungi. hBDs can cause membrane depolarization and cell lysis due to their highly cationic nature. These molecules participate in antimicrobial defenses and the control of adaptive and innate immunity in every mammalian species and are produced by various cell
Biologically active cell penetrating peptides (CPPs) are an emerging class of therapeutic agent. The wasp venom peptide mastoparan is an established CPP that modulates mitochondrial activity and triggers caspase-dependent apoptosis in cancer cells, as does the mastoparan analogue mitoparan (mitP). Mitochondrial depolarisation and activation of the caspase cascade also underpins the action of dithranol, a topical agent for treatment of psoriasis. The effects of a potent mitP analogue on mitochondrial activity were therefore examined to assess its potential as a novel approach for targeting mitochondria for the treatment of psoriasis. In HaCaT keratinocytes treated with the mitP analogue Z-Gly-RGD(DPhe)-mitP for 24 h, a dose-dependent loss of mitochondrial activity was observed using the methyl-thiazolyl-tetrazolium (MTT) assay. At 10 μmol L−1, MTT activity was less than 30% that observed in untreated cells. Staining with the cationic dye JC-1 suggested that Z-Gly-RGD(DPhe)-mitP also dissipated the
Publisher: University of Delaware. Date Issued: 2015. Abstract: Structural mechanisms and underlying thermodynamic determinants of efficient internalization of charged cationic peptides (cell-penetrating peptides, CPPs) such as TAT, polyarginine, and their variants, into cells, cellular constructs, and model membrane/lipid bilayers (large and giant unilamellar or multilamelar vesicles) continue to garner significant attention. Two widely-held views on the translocation mechanism center on endocytotic and non-endocytotic (diffusive) processes. Using the translocation of short, charged cationic oligo-arginine peptides (mono-, di-, and tri-arginine) from bulk aqueous solution into model DMPC bilayers, we explore the question of the similarity of thermodynamic and structural predictions obtained from molecular dynamics simulations using all-atom and the Martini coarse-grain force fields. Specifically, we estimate potentials of mean force associated with translocation using standard all-atom ...
Gastrulation is a key transition in embryogenesis; it requires self-organized cellular coordination, which has to be both robust to allow efficient development and plastic to provide adaptability. Despite the conservation of gastrulation as a key event in Metazoan embryogenesis, the morphogenetic mechanisms of self-organization (how global order or coordination can arise from local interactions) are poorly understood. We report a modular structure of cell internalization in Caenorhabditis elegans gastrulation that reveals mechanisms of self-organization. Cells that internalize during gastrulation show apical contractile flows, which are correlated with centripetal extensions from surrounding cells. These extensions converge to seal over the internalizing cells in the form of rosettes. This process represents a distinct mode of monolayer remodeling, with gradual extrusion of the internalizing cells and simultaneous tissue closure without an actin purse-string. We further report that this self-organizing
Due to their enhanced stability and cell permeability, cyclic cell-penetrating peptides have been widely used as delivery vectors for transporting cell-impermeable cargos into cells. In this study, we synthesized a panel of conformationally constrained peptides with either α-helix or β-hairpin conformations.
Zuschriften DOI: 10.1002/ange.201104514 Delivery Platforms Oligonucleotide Delivery by Cell-Penetrating Striped Nanoparticles** Christopher M. Jewell, Jin-Mi Jung, Prabhani U. Atukorale, Randy P. Carney, Francesco Stellacci,* and Darrell J. Irvine* Gold nanoparticles (AuNPs) hold great interest in drug delivery because these materials can be functionalized with a range of biological cargos, induce minimal toxicity, and can be efficiently cleared from the body.[1] For example, many studies have described conjugation of DNA or RNA to particle surfaces in well-defined configurations, and these materials have been applied in numerous biological and therapeutic settings.[1, 2] Devising new ways to mediate cell entry by AuNPs is a central area of interest. When mixed self-assembled monolayers of dislike molecules are used to coat AuNPs, nanoscale domains spontaneously form in the particles ligand shell. In particular, stripe-like domains form for ca. 1:1 binary mixed ligand compositions.[3] The ...
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Frank Cady, character actor, dies at 96; Don Campbell, author, dies at 65; Frank Arciero, developer, dies at 86; F. Herbert Bormann, acid rain discoverer, dies at 90
Viral particles derived from mouse polyomavirus can be potentially used as a delivery system for therapeutic genes and drugs into target cells. This thesis focuses on preparation and characterization of polyomaviral particles that are modified with cell-penetrating peptides in order to increase efficiency of transduction of reporter genes into human cells. Viral particles that are composed of major capsid protein VP1 in combination with minor capsid protein VP2 and minor capsid protein VP3 that is modified with octaarginine, LAH4 peptide or with transduction domain of adenoviral protein VI are analysed in transduction assays. The thesis also provides information about the effect of the modification on encapsidation of heterologous DNA. The results of transduction assays performed with modified particles containing encapsidated luciferase gene revealed that efficiency of transduction did not increase but decreased in comparison with unmodified particles. These findings help to elucidate the role ...
A novel solid-state NMR technique for identifying the asymmetric insertion depths of membrane proteins in lipid bilayers is introduced. By applying Mn2+ ions on the outer but not the inner leaflet of lipid bilayers, the sidedness of protein residues in the lipid bilayer can be determined through paramagnetic relaxation enhancement (PRE) effects. Protein-free lipid membranes with one-side Mn2+-bound surfaces exhibit significant residual 31P and lipid headgroup 13C intensities, in contrast to two-side Mn2+-bound membranes, where lipid headgroup signals are mostly suppressed. Applying this method to a cell-penetrating peptide, penetratin, we found that at low peptide concentrations, penetratin is distributed in both leaflets of the bilayer, in contrast to the prediction of the electroporation model, which predicts that penetratin binds to only the outer lipid leaflet at low peptide concentrations to cause an electric field that drives subsequent peptide translocation. The invalidation of the ...
Cellular introduction of PEBBLEs (photonic explorers for bioanalysis with biologically localized embedding) has been investigated by a wide variety of methods in a range of cell types. These methods include surface functionalization with CPPs (cell-penetrating peptides), pinocytosis, commercial lipid transfection agents, cytochalasin D, picoinjection, and Gene gun bombardment. This paper will overview several of the most popular methods used for the introduction of PEBBLE nanosensors to the cellular environment and discuss the efficacy of the techniques.. ...
This is a short cell-penetrating peptide (CPP) derived from the venom peptide of Tunician scorpion. It can permeate cell membrane at low micromolar concentration in minutes without significantly affecting cell membrane. Maurocalcine can be conjugated with large molecules and used as a drug delivery vehicle.
In recent years, significant research efforts have been dedicated to improving the treatment options and visual outcomes of patients with age-related macular degeneration (AMD). The introduction of VEGF inhibitors offered a means of preventing permanent vision loss, and even improving vision. However, the necessity of monthly or bimonthly intravitreal injections still remains. Presentations at ARVO 2015 highlighted several novel delivery systems in development to address this limitation.. DeCogan et al presented a de novo-designed system of cell-penetrating peptide constructs fused to a therapeutic protein transduction domain (DeCogan F: 4147). In vivo, the system successfully transported microgram quantities of macromolecules, such as the large monoclonal VEGF inhibitors, in a topically administered drop that penetrated to the back of the eye.. Other presentations focused on slow-release depots of VEGF inhibitors. Initial in vitro pharmacokinetic work was presented on controlled-release polymer ...
A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK The authors showed that a cell-penetrating peptide based on CX43 inhibited c-Src and focal adhesion kinase (FAK) and upregulated phosphatase and tensin homolog in glioma stem cells derived from patients. [Stem Cell Reports] Full Article BAG3 Promotes Stem Cell-Like Phenotype in Breast Cancer by Upregulation of CXCR4 via Interaction with its Transcript Scientists reported that BAG3 was induced under specific floating culture conditions that enrich breast CSC-like cells in spheres. Ectopic BAG3 overexpression increased CD44+/CD24− CSC subpopulations, first-generation and second-generation mammosphere formation, indicating that BAG3 promotes CSC self-renewal and maintenance in breast cancer. [Cell Death Dis] Full Article Aptamer-Mediated Survivin RNAi Enables 5-Fluorouracil to Eliminate Colorectal Cancer Stem Cells Researchers showed that EpCAM-aptamer-guided survivin RNAi ...
UNLABELLED: A prominent feature of many human cancers is oncogene-driven activation of the DNA damage response (DDR) during early tumorigenesis. It has been shown previously that noninvasive imaging of the phosphorylated histone H2A variant H2AX, γH2AX, a DNA damage signaling protein, is possible using (111)In-labeled anti-γH2AX antibody conjugated to the cell-penetrating peptide transactivator of transcription (TAT). The purpose of this study was to investigate whether (111)In-anti-γH2AX-TAT detects the DDR during mammary oncogenesis in BALB-neuT mice. METHODS: Mammary fat pads from BALB-neuT and wild-type mice (age, 40-106 d) were immunostained for γH2AX. (111)In-anti-γH2AX-TAT or a control probe was administered intravenously to BALB-neuT mice. SPECT was performed weekly and compared with tumor detection using palpation and dynamic contrast-enhanced MR imaging. RESULTS: γH2AX expression was elevated in hyperplastic lesions in the mammary fat pads of BALB-neuT mice aged 76-106 d, compared with
In this dissertation, a novel cell penetrating peptide (CPP)-based polyplex was developed and evaluated for small interfering RNA (siRNA) delivery and efficacy. The design resolved the CPP carrier neutralization issue, where CPP cellular uptake is compromised by electrostatic interactions between the CPP and siRNA, by using a chemically modified 21mer oligolysine (K21-PDP) to form a stable and neutralized polyplex with siRNA. This neutralized polyplex served as a suitable platform to conjugate distinct CPP carrier moieties - cationic (hexaarginine, R6) and amphipathic (model amphipathic peptide, MAP) - to determine which CPP property was more suitable for siRNA delivery. Since K21-PDP already effectively neutralized the siRNA, the CPP carriers on the conjugated CPP-polyplexes, R6-polyplex and MAP-polyplex, were able to function without electrostatic interference. Amphipathic MAP was found to be a better CPP carrier than R6 because MAP-polyplex exhibited greater siRNA intracellular uptake and ...
Neurological diseases such as neurodegeneration, pain, psychiatric disorders, stroke, and brain cancers would greatly benefit from the use of highly potent and specific peptide pharmaceuticals. Peptides are especially desirable because of their low inherent toxicity. The presence of the blood brain barrier (BBB), their short duration of action, and their need for parenteral administration limits their clinical use. However, over the past decade there have been significant advances in delivering peptides to the central nervous system. Angiopep peptides developed by Angiochem (Montreal, Canada), transferrin antibodies developed by ArmaGen (Santa Monica, USA), and cell penetrating peptides have all shown promise in delivering therapeutic peptides across the BBB after intravenous administration. Noninvasive methods of delivering peptides to the brain include the use of chitosan amphiphile nanoparticles for oral delivery and nose to brain strategies. The uptake of the chitosan amphiphile ...
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) exhibits the poorest prognosis of all solid tumors with a 5-year survival rate of less than 10% and a median survival of 6 months after diagnosis. Numerous targeted agents have been developed and evaluated to improve the survival benefit in patients with PDAC. Unfortunately, most agents have been proven futile mainly owing to the dense stroma and the sophisticated signaling pathways of PDAC. Here, we show the potent effectiveness of Aptamer-SH2 superbinder-(Arg)9 conjugate on the treatment of PDAC. In this conjugate, DNA aptamer selected against PDAC cell line confers the function of specifically recognizing and binding to the PDAC cells and activated pancreatic stellate cells (PSCs) in stroma; cell penetrating peptide (Arg)9 facilitates the intracellular delivery of fused proteins; SH2 superbinder conducts the drastic blockade of multiple phosphotyrosines (pY)-based signaling pathways in tumor cells. METHODS: PDAC-associated pY were reanalyzed ...
Ashwanikumar et al have developed a drug delivery system using cell-penetrating self-assembling peptide nanomaterials (CSPNs); these drilled-shaped nanomaterials traverse cellular membranes to deliver drugs and can be fine-tuned into different shapes to optimize delivery. Image credit: Ashwanikumar et al, doi: 10.1016/j.jconrel.2018.02.041.. Developing effective therapeutic drugs is an arduous process that involves a large investment of time and money even before clinical trials begin.. Often, drugs that should theoretically treat a disease do not reach clinical trials because the drugs fail to penetrate biological barriers such as cellular membranes and reach the desired molecular target.. Scientists at Oregon State University have developed a new nanomaterial that facilitates the delivery of drugs through cell membrane which they call CSPNs, or cell-penetrating self-assembling peptide nanomaterials.. Composed of amino acids that self-assemble into a shape akin to a drill bit, these ...
Providing academic quality information that is approachable to patients, families and other stakeholders in the field of chronic pain rehabilitation.
We were pleased to welcome Dr. Blake Cady as our visiting professor and judge. Drs. Desmond Birkett, Patricia Donahoe, and Steven Schwaitzberg joined him as judges. This year there were many more submissions than could be presented today demonstrating the continued enthusiasm for surgical research here in New England. ...
Cady here. Im glad Mommy and Daddy got a weekend away. I know they really needed it. Mom said she was going on vacation and I was getting a Stay-cation. Im not totally sure what that it, but I know I like it and Mommy and Daddy can go away like that any time. Nana…
Lerner, E. B., Willenbring, B. D., Pirrallo, R. G., Brasel, K. J., Cady, C. E., Colella, M. R., Cooper, A., Cushman, J. T., Gourlay, D. M., Jurkovich, G. J., Newgard, C. D., Salomone, J. P., Sasser, S. M., Shah, M. N., Swor, R. A. & Wang, S. C., Apr 2014, In : Journal of Trauma and Acute Care Surgery. 76, 4, p. 1157-1163 7 p.. Research output: Contribution to journal › Article ...
The Young and the Restless spoilers reveal that Kellys (Cady McClain) craziness could reach a new extreme in the near future.
Sigma-Aldrich offers abstracts and full-text articles by [M Lahn, H Kalataradi, P Mittelstadt, E Pflum, M Vollmer, C Cady, A Mukasa, A T Vella, D Ikle, R Harbeck, R OBrien, W Born].
SS Pauliah, PJ Lally, DL Price, A Bainbridge, J Kurien, N Sivaswami, FM Cowan, G Balraj, R Swamy, V Madhavan, M Nair, P Krishnakumar, EB Cady, S Shankaran, S Thayyil ...
Over the next number of weeks, Brian Cady will be joined on Cadys Corner every Saturday morning at around 10:15 by a representative from the Capital District Basketball Association, the only NCAA-sanctioned summer league in the Capital Region and one of only about 70 in the entire count ...
C. M. Jewell, Jung, J. - M., Atukorale, P. U., Carney, R. P., Stellacci, F., and Irvine, D. J., Oligonucleotide Delivery by Cell-Penetrating Striped Nanoparticles, Angewandte Chemie-International Edition, vol. 50, no. 51, pp. 12312 - 12315, 2011. ...
C. M. Jewell, Jung, J. - M., Atukorale, P. U., Carney, R. P., Stellacci, F., and Irvine, D. J., Oligonucleotide Delivery by Cell-Penetrating Striped Nanoparticles, Angewandte Chemie-International Edition, vol. 50, no. 51, pp. 12312 - 12315, 2011. ...
Bring it on: Organic chemistry on surfaces and in solution is not the same; this study offers a perfect example, with small changes (from 27 to 35°; see graphic) and big consequences. Strained cyclic disulfides from asparagusic, but not lipoic acid, are ideal for growing functional architectures directly on surfaces; for the substrate-initiated synthesis of cell-penetrating poly(disulfide)s in solution, exactly the contrary is true.. [open archive unige:29562 • pdf ...
Recent breakthroughs in gene editing have necessitated practical ex vivo methods to rapidly and efficiently re-engineer patient-harvested cells. Many physical membrane-disruption or pore-forming techniques for intracellular delivery, however, result in poor cell viability, while most carrier-mediated techniques suffer from suboptimal endosomal escape and hence cytoplasmic or nuclear targeting. In this work, we show that short exposure of cells to high frequency (>10 MHz) acoustic excitation facilitates temporal reorganisation of the lipid structure in the cell membrane that enhances translocation of gold nano- particles and therapeutic molecules into the cell within just ten minutes. Due to its transient nature, rapid cell self-healing is observed, leading to high cellular viabilities (>97%). Moreover, the internalised cargo appears to be uniformly distributed throughout the cytosol, circumventing the need for strategies to facilitate endosomal escape. In the case of siRNA delivery, the method ...
Weng, A, et al. (2015) Improved intracellular delivery of peptide- and lipid-nanoplexes by natural glycosides. J Control Release. 2015 May 28; 206:75-90. PM ID: ...
Bachem offers H-7514 Penetratin for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
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MANILA, Philippines-Former President and now Pampanga Representative Gloria Macapagal-Arroyo went to the Makati Medical Center (MMC) Thursday afternoon to undergo a digital mammogram.. Arroyo left the Veterans Memorial Medical Center (VMMC), where she is under hospital detention for plunder, around noon and arrived at the Makati hospital at 1 p.m. in a wheelchair and wearing a neck brace ...
Cell-penetrating peptide CPP: guides the TIVA tag through cell membranes into tissues. It is linked to the TIVA tag by a ... Madani F, Lindberg S, Langel U, Futaki S, Gräslund A (2011). "Mechanisms of cellular uptake of cell-penetrating peptides". ... TIVA tags are created initially via solid-phase synthesis with the cell-penetrating peptide conjugated afterwards. The ... the cell is imaged with the confocal microscope. Using a glass pipette, the photolysed cell is isolated by aspiration. Cells ...
Two features of the protein structure have been associated with cell-penetrating properties that are uncommon for peptide ... Although the toxin was discovered to be cell-penetrating, there is no sequence similarity to classical cell-penetrating ... Guidotti, Giulia; Brambilla, Liliana; Rossi, Daniela (April 2017). "Cell-Penetrating Peptides: From Basic Research to Clinics ... "A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain". Cell. 178 (6): 1362-1374.e16. doi: ...
"Human DMBT1-Derived Cell-Penetrating Peptides for Intracellular siRNA Delivery". Molecular Therapy - Nucleic Acids. 8: 264-276 ... Cell Mol. Biol. 20 (4): 759-68. doi:10.1165/ajrcmb.20.4.3439. PMID 10101009. Holmskov U, Lawson P, Teisner B, Tornoe I, Willis ... Cell Mol. Biol. 20 (4): 759-68. doi:10.1165/ajrcmb.20.4.3439. PMID 10101009. Holmskov U, Mollenhauer J, Madsen J, Vitved L, ... The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with ...
Seo, Bong; Hong, Yean; Do, Jeong (2017-03-03). "Cellular Reprogramming Using Protein and Cell-Penetrating Peptides". ... One of the available options is the transfer of small proteins or penetrating peptides across the membrane, which is safer but ... in 2012 in relation to the effectiveness of reprogramming somatic cells to pluripotent cells using viral and non-viral ... This phenomenon is essential in dedifferentiating a somatic cell to a pluripotent cell (induction of an induced pluripotent ...
... appears to be an even more potent cell penetrating peptide. A Hadrucalcin derivative has been used as a carrier for ... Cell-Penetrating Nanobiosensors for Pointillistic Intracellular Ca 2+ - Transient Detection. 2014.. ... Hadrucalcin is a peptide toxin from the scorpion calcin family. The mature hadrucalcin peptide is composed of 35 amino acids, ... Cell penetration properties of a highly efficient mini maurocalcine Peptide. Pharmaceuticals (Basel). 2013;6(3):320-39. doi: ...
... calcins are able to penetrate cell membranes with high efficiency. Thus, they act as cell-penetrating peptides (CPPs) and can ... It acts as a cell-penetrating peptide (CPP); it binds with high affinity and specificity to skeletal ryanodine receptor 1 (RYR1 ... Cell penetration properties of maurocalcine, a natural venom peptide active on the intracellular ryanodine receptor. Biochim. ... a Family of High-Affinity Peptide Ligands of Ryanodine Receptors". Biophysical Journal. Cell. 106 (2): 106-13. doi:10.1016/j. ...
"Predicting cell-penetrating peptides using machine learning algorithms and navigating in their chemical space". Scientific ... of five for the implicit assumption that passive diffusion is the only important mechanism for the entry of drugs into cells, ... also demonstrated that some natural products break the chemical rules used in Lipinski filter such as macrolides and peptides ...
... s are cell-penetrating peptides that act as intracellular modulators of signal transference from receptors to G ... "Activation and inhibition of G protein-coupled receptors by cell-penetrating membrane-tethered peptides". Proc. Natl. Acad. Sci ... "Suppression of Arterial Thrombosis with Affecting Hemostatic Parameters with a Cell-Penetrating PAR1 Pepducin". Circulation. ... A pepducin molecule consists of a short peptide derived from a GPCR intracellular loop tethered to a hydrophobic moiety. This ...
The iRGD peptide homes and penetrates tumors through a 3-step process: First, the RGD sequence motif mediates binding to αVβ3 ... Teesalu T, Sugahara KN, Kotamraju VR, Ruoslahti E (September 2009). "C-end rule peptides mediate neuropilin-1-dependent cell, ... The peptide was able to home to tumor tissues, but in contrast to standard RGD (Arginylglycylaspartic acid) peptides, also ... Ruoslahti E (February 2017). "Tumor penetrating peptides for improved drug delivery". Advanced Drug Delivery Reviews. 110-111 ( ...
Scientific Reports 3: 1607 CellPPD: In silico approaches for designing highly effective cell penetrating peptides. Journal of ... PLoS ONE 8(6): e67008 Lbtope: Improved Method for Linear B-Cell Epitope Prediction Using Antigen's Primary Sequence. PLoS ONE 8 ... In Silico Approach for Predicting Toxicity of Peptides and Proteins. [1] "Notable bioinformatician - Dr Gajendra Pal Singh ... Computational approach for designing tumor homing peptides. ...
Tat contains a protein transduction domain, which is therefore known as a cell-penetrating peptide. Originally characterised by ... It can be absorbed by cells that are not infected with HIV, and can act directly as a toxin producing cell death via apoptosis ... Schwarze SR, Hruska KA, Dowdy SF (July 2000). "Protein transduction: unrestricted delivery into all cells?". Trends Cell Biol. ... this domain allows Tat to enter cells by crossing the cell membrane. The amino acid sequence of the protein transduction domain ...
"A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library". Bioorganic & Medicinal Chemistry. 10 ... Further mechanistic studies of this peptide uncovered a dimerization "switch" that modulates the cell-penetrating activity. In ... By panning this library against a B lymphoctye cell line, a unique cell-binding and internalizing peptide was discovered. ... in the Internalization Mechanism of a Cell-Penetrating Peptide". Journal of the American Chemical Society. 127 (2): 538-539. ...
"Cell-Penetrating Peptide-Modified Gold Nanoparticles for the Delivery of Doxorubicin to Brain Metastatic Breast Cancer". ... They concluded a 15% cell viability and dose dependent cell damage. Reduction in cell viability was detected in vivo ... Studies in human leukemia cells revealed that prolonged exposure in AuNPs did not harm the cells, even at ~100 μM of Au. Rather ... A tumor consists of a multitude of cell types, and thus targeting a single type of cell is ineffective and potentially ...
"Stability of cell-penetrating peptide-morpholino oligomer conjugates in human serum and in cells". Bioconjugate Chemistry. 18 ( ... cells in adult organisms can be accomplished by using covalent conjugates of Morpholino oligos with cell-penetrating peptides, ... stability and toxicity of a cell-penetrating peptide-morpholino oligomer conjugate". Bioconjugate Chemistry. 18 (4): 1325-31. ... the minor spliceosome acts outside the nucleus and controls cell proliferation". Cell. 131 (4): 718-29. doi:10.1016/j.cell. ...
A disulfide-linked peptide, which is adjacent to the tag, allows it to penetrate the cell membrane. Once inside, laser ... single-cell RNA sequencing generates the transcriptome of the visualized cells. This method can process thousands of cells ... Single cell sequencing Single-cell transcriptomics RNA-Seq Fluorescence in situ hybridization Ståhl PL, Salmén F, Vickovic S, ... The cells bound by the fluorescent marker are photoactivated, dissociated and sorted via fluorescence-activated cell sorting. ...
Morpholino antisense oligos conjugated to cell penetrating peptides have been shown to partially protect mice from WNV disease ... West Nile meningitis (WNM) usually involves fever, headache, stiff neck and pleocytosis, an increase of white blood cells in ... Disconnect of upper motor neuron influences on the anterior horn cells possibly by myelitis or glutamate excitotoxicity have ...
... sulfate proteoglycans cooperate in cellular uptake of nanoparticles functionalized by cationic cell-penetrating peptides". Sci ... The prototype tumor-penetrating peptide, iRGD, is in clinical trials in solid tumor patients tested as an enhancer of cancer ... These tumor-penetrating peptides selectively home to tumor vessels, where they activate a transport pathway that delivers the ... Teesalu, T.; Sugahara, K. N.; Kotamraju, V. R.; Ruoslahti, E. (2009). "C-end rule peptides mediate neuropilin-1-dependent cell ...
... a nontoxic protamine substitute and an effective cell-penetrating peptide". Journal of Controlled Release. 193: 63-73. doi: ... A sperm cell can contain up to 50,000 toroid-shaped structures in its nucleus with each toroid containing about 50 kilobases. ... The primary structure of protamine P1, the protamine used for packaging DNA in sperm cells, in placental mammals is usually 49 ... An analogue of this peptide has also been produced. In gene therapy, protamine sulfate's ability to condense plasmid DNA along ...
... and cell-penetrating anti-fungal peptides. Penicillium digitatum can be identified in the laboratory using a variety of methods ... "Understanding the mechanism of action of cell-penetrating antifungal peptides using the rationally designed hexapeptide PAF26 ... The hyphal cells are haploid, although individual hyphal compartments may contain many genetically identical nuclei. During the ... Revealing diverse modes of action and biological roles of antifungal peptides. 26 (4): 146-155. doi:10.1016/j.fbr.2012.10.003. ...
"Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E". RSC Advances. 5 (26). ... In the area of nanotechnology, Igor V. Komarov's research group studied cell-penetrating peptides as carriers for carbon-based ... with a purpose of using them as labels to study peptides in lipid bilayers by solid-state NMR spectroscopy. Igor V. Komarov's ... photocontrollable peptides - potential candidates for photopharmacology drugs. Photopharmacology drugs can be administered in ...
The capsid protein L2 disrupts the membrane of the endosome through a cationic cell-penetrating peptide, allowing the viral ... Together with E6, E7 serves to prevent cell death (apoptosis) and promote cell cycle progression, thus priming the cell for ... This tethering to the cell's nuclear matrix ensures faithful distribution of viral genomes to each daughter cell after cell ... "Cell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde ...
proteases will hydrolyze peptide bonds; secreted aspartic proteases (SAP) support C. tropicalis to be attached and penetrate ... C. tropicalis diploid cells of opposite mating type can mate to form tetraploid cells. These cells may then undergo chromosome ... Such diploid cells are again mating competent thus completing a parasexual cycle. Opaque C. tropicalis cells can also form an ... Eukaryot Cell. 2013 Dec;12(12):1629-40. doi: 10.1128/EC.00128-13. PMID 24123269 Jones SK Jr, Hirakawa MP, Bennett RJ. Sexual ...
... because their positive charges assist binding on the brain's endothelial cells. Using TAT-peptides, a cell-penetrating peptide ... By transfection of endothelial cells through the use of lipoplexes, physical alterations in the cells could be made. These ... a type of cell-penetrating protein, for anti-glioma therapy. Once injected into the patient's body, the albumin nanoparticles ... bind to the proteins and penetrate glioma cells, and then release the contained drugs. This nanoparticle formulation enhances ...
This allows for a more stable two-pronged attachment, which allows the N-terminal fusion peptide gp41 to penetrate the cell ... "cell-to-cell spread". In cell-free spread (see figure), virus particles bud from an infected T cell, enter the blood or ... Cell-to-cell spread is thought to be particularly important in lymphoid tissues where CD4+ T cells are densely packed and ... HIV infects vital cells in the human immune system, such as helper T cells (specifically CD4+ T cells), macrophages, and ...
Walker L, Perkins E, Kratz F, Raucher D (October 2012). "Cell penetrating peptides fused to a thermally targeted biopolymer ... for metastatic small cell lung cancer Treatment of advanced or metastatic pancreatic ductal adenocarcinoma Comparison of ... "Efficacy and Safety of Aldoxorubicin Compared to Topotecan in Subjects With Metastatic Small Cell Lung Cancer" at ...
Hsu, T.; Mitragotri, S. (8 September 2011). "Delivery of siRNA and other macromolecules into skin and cells using a peptide ... skin penetrating peptides and ionic liquids. He also invented intestinal patches and ionic liquids for oral delivery of ... Mitragotri also pioneered nanoparticle-enabled cell therapies which use drug-loaded nanoparticles that hitch a ride on red ... blood cells, monocytes and other circulatory cells for tissue-specific delivery. Mitragotri's technologies are used to develop ...
... systems can also be empowered to cross the cellular membrane by conjugating with cell penetrating molecules such as peptides ... tumor cell suppressor p53 and cytochrome C can be delivered into cancer cells efficiently. For biomolecules that are not ... Veronese, Francesco M (March 2001). "Peptide and protein PEGylation". Biomaterials. 22 (5): 405-417. doi:10.1016/s0142-9612(00) ... cell sorting, and clinical diagnostics. Microcapsules termed protocells prepared by polymer-protein hybrids are the hotspot of ...
Verdine received a research grants to study cell-penetrating mini-proteins in order to target cancer cells.[3] His work has led ... A close analog of a stapled peptide drug invented in the Verdine Lab at Harvard is currently in Phase II clinical development ... a prominent new class of drugs that combine the versatile binding properties of monoclonal antibodies with the cell-penetrating ... He continues to run[7] his eponymous Verdine Laboratory at the Harvard University Department of Stem Cell & Regenerative ...
... cell body) Periglomerular cells in the Olfactory bulb Cerebellar cortex Neurons in the brainstem, entorhinal and pyriform ... Peptide ion channel blockers Low molecular weight compounds Therapeutics There are only two peptide toxins that selectively ... One advantage of low molecular weight channel blockers is that they can penetrate tissue, which is important for crossing the ... located within the dendrites of the Purkinje cells. Without calcium flux in the Purkinje cells, action potentials fire ...
This cell-penetrating peptide selectively activates mammalian TRPA1 receptors, which are expressed in pain-sensing peripheral ... "A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain". Cell. 178 (6): 1362-1374.e16. doi: ... U. manicatus was recently shown to harbor a unique peptide toxin, presumably for defensive purposes. ... 10.1016/j.cell.2019.07.014. ISSN 1097-4172. PMC 6731142. PMID 31447178. Koch, L.E. (1977). The taxonomy, geographic ...
... a whole-cell sonicate enzyme immunoassay followed by a VlsE C6 peptide enzyme immunoassay". Clinical Infectious Diseases. 53 (6 ... "penetrates the skin". Many people from this area of Great Britain emigrated to North America between 1717 and the end of the ... The spirochetes may also induce host cells to secrete quinolinic acid, which stimulates the NMDA receptor on nerve cells, which ... However, PCR tests are susceptible to false positive results, e.g. by detection of debris of dead Borrelia cells or specimen ...
Targeting double-stranded breaks increases the probability that cells will undergo cell death. Cancer cells are generally less ... It was found that while superficial tumors could be treated with low voltage x-rays, more penetrating, higher energy beams were ... peptide receptor radionuclide therapy). ... Tumor cells in a hypoxic environment may be as much as 2 to 3 ... Fractionation allows normal cells time to recover, while tumor cells are generally less efficient in repair between fractions. ...
They develop hyphae, threadlike structures that penetrate the host cell. In mutualistic situations the plant often exchanges ... antimicrobial peptides and more". Animal Conservation. 10 (4): 425-8. doi:10.1111/j.1469-1795.2007.00150.x.. ... Ten Times More Microbial Cells than Body Cells in Humans? *^ Alison Abbott for Nature News. Jan 8 2016 Scientists bust myth ... doi:10.1016/j.cell.2006.02.017. PMID 16497592.. *^ a b Salvucci, E. (2016). "Microbiome, holobiont and the net of life". ...
Sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society. 16 (3): 230-6. doi: ... When ultraviolet rays penetrate the skin and damage DNA, thymidine dinucleotide (pTpT) fragments from damaged DNA will trigger ... including the MSH and ACTH peptides that are produced from the precursor proopiomelanocortin. ... Markert CL and Silvers WK (1956). "The effects of genotype and cell environment on melanoblast differentiation in the house ...
Tat contains a protein transduction domain, which is therefore known as a cell-penetrating peptide.[13] Originally[14] ... It can be absorbed by cells that are not infected with HIV, and can act directly as a toxin producing cell death via apoptosis ... Schwarze SR, Hruska KA, Dowdy SF (July 2000). "Protein transduction: unrestricted delivery into all cells?". Trends Cell Biol. ... this domain allows Tat to enter cells by crossing the cell membrane. The amino acid sequence of the protein transduction domain ...
Calcein-AM, A highly permeable derivative of calcein readily penetrates into intact cells, where the endogenous esterases ... Family 3.A.1.111 The Peptide-1 Exporter (Pep1E) Family 3.A.1.112 The Peptide-2 Exporter (Pep2E) Family 3.A.1.113 The Peptide-3 ... family 3.A.1.111 Peptide-1 exporter (Pep1E) family 3.A.1.112 Peptide-2 exporter (Pep2E) family 3.A.1.113 Peptide-3 exporter ( ... In multidrug-resistant cells, the MDR1 gene is frequently amplified in multidrug-resistant cells. This results in a large ...
B-flow is a mode that digitally highlights moving reflectors (mainly red blood cells) while suppressing the signals from the ... Harmonic mode: In this mode a deep penetrating fundamental frequency is emitted into the body and a harmonic overtone is ... Ultrasound Imaging of Tumor Angiogenesis with Contrast Microbubbles Conjugated to Integrin-Binding Knottin Peptides". Journal ... Specifically, sound is reflected anywhere where there are acoustic impedance changes in the body: e.g. blood cells in blood ...
... contained in the central cell) in the centre of the gametophyte. The resulting cell is triploid (3n). This triploid cell ... In seed plants, after pollination, a pollen grain germinates, and a pollen tube grows and penetrates the ovule through a tiny ... Resact is a 14 amino acid peptide purified from the jelly coat of A. punctulata that attracts the migration of sperm. ... In flowering plants a second fertilisation event involves another sperm cell and the central cell which is a second female ...
"A Noncovalent Peptide-Based Strategy for Peptide and Short Interfering RNA Delivery". Handbook of Cell-Penetrating Peptides, ... prediction of cell-penetrating peptides Bioinformatics (2013) 29 (23): 3094-3096... *CellPPDMod: Prediction of cell-penetrating ... Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake/uptake of various molecular equipment (from ... "A New Potent Secondary Amphipathic Cell-penetrating Peptide for siRNA Delivery into Mammalian Cells". Molecular Therapy. 17 (1 ...
... innate immune cells, including immature dendritic cells (DCs), natural killer (NK) cells, and T cells, as well as (5) their ... Pulpitis may be caused by dental caries that penetrate through the enamel and dentin to reach the pulp, or it may be a result ... Other peptides include cGRP, galanin, somatostatin, and neurokinin A-B. The biological effects of SP are expressed by the ... B cells and T cells are the major lymphocytes involved.[10]. A variety of cytokines have been observed in the pulp. Patients ...
Interstitial kidney cells. Gene and protein expression[edit]. Further information: Bioinformatics § Gene and protein expression ... Each renal artery branches into segmental arteries, dividing further into interlobar arteries, which penetrate the renal ... and atrial natriuretic peptide, among others. ... Kidney proximal tubule brush border cell. *Loop of Henle thin ... It stimulates erythropoiesis (production of red blood cells) in the bone marrow. Calcitriol, the activated form of vitamin D, ...
... but even penetrates into the cell. Its binding to the bacteria wall is associated with the specific peptide lactoferricin, ... Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell. Lactoferrin binds to the ... Lactoferrin also suppresses virus replication after the virus penetrated into the cell. Such an indirect antiviral effect is ... Cell Biol. 80 (1): 125-30. doi:10.1139/o01-208. PMID 11908636. Harmsen MC, Swart PJ, de Béthune MP, Pauwels R, De Clercq E, The ...
Antimicrobial peptides[edit]. The skin creates antimicrobial peptides such as cathelicidins that control the proliferation of ... If activated, the immune system in the skin produces cell-mediated immunity against microbes such as dermatophytes (skin fungi ... dermatitis provoked by microorganisms and allergens penetrating the corneal layer and redness. Wearing gloves can cause further ... Psoriasis has been linked to self-DNA created from cathelicidin peptides that causes autoinflammation. A major factor ...
Alphabodies, also known as Cell-Penetrating Alphabodies or CPAB for short, are small 10 kDa proteins engineered to bind to a ... Initially, the scaffold was made of three peptides that associated non-covalently to form a parallel coiled-coil trimer. ... However, the scaffold was later redesigned as a single peptide chain containing three α-helices connected by linker regions. ... Desmet, J.; Lasters, I. (2010). "Non-natural proteinaceous scaffold made of three non-covalently associated peptides". US ...
... they cannot penetrate cell membranes. Thus, GH exerts some of its effects by binding to receptors on target cells, where it ... These cells release the peptides growth hormone-releasing hormone (GHRH or somatocrinin) and growth hormone-inhibiting hormone ... cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH also ... Growth hormone (GH) or somatotropin, also known as human growth hormone (hGH or HGH) in its human form, is a peptide hormone ...
Hepatocyte and epithelial cell proliferation. *Expression of antimicrobial peptides. *Expression of chemotactic cytokines ... Sharp, small foreign bodies can penetrate the skin leaving little surface wound but causing internal injury and internal ... Stem cells give rise to progenitor cells, which are cells that are not self-renewing, but can generate several types of cells. ... Macrophages are a type of repairing cell that devour dead cells and pathogens, and trigger other immune cells to respond to ...
"Cell. 148 (6): 1204-22. doi:10.1016/j.cell.2012.02.040. PMC 3319071. PMID 22424230.. ... Exactly how disturbances of production and aggregation of the beta-amyloid peptide give rise to the pathology of AD is not ... APP is a transmembrane protein that penetrates through the neuron's membrane. APP is critical to neuron growth, survival, and ... While scientists know Alzheimer's disease involves progressive brain cell failure, the reason cells fail isn't clear.. ...
The virus is injected along with the wasp egg into the body cavity of a lepidopteran host caterpillar and infects cells of the ... The wasp Leptopilina heterotoma secrete VLPs that are able to penetrate into the lamellocytes, thanks to specific receptors, ... Finally, polyDNAvirus can also produce viral ankyrins, that interfere with production of antiviral peptides.[13] In some ... V. canescens-VLPs (VcVLP1, VcVLP2, VcNEP …) are produced in the calix cells before they go to the oviducts. But they are ...
... small blue cells - juxtaglomerular cells; 4) large blue cells - mesangial cells; 5) tan - podocytes lining Bowman's capsule ... atrial natriuretic peptide (sodium) and brain natriuretic peptide (sodium). A countercurrent system in the renal medulla ... Cortical nephrons (the majority of nephrons) start high in the cortex and have a short loop of Henle which does not penetrate ... It contains three components: the macula densa, juxtaglomerular cells, and extraglomerular mesangial cells.[citation needed] ...
Microneedles of approximately 100μm can penetrate the skin barrier and deliver drugs to the underlying cells and interstitial ... Peptides, on the other hand, are more chemically resistant and can retain partial aspects of protein function. As such, peptide ... Cell fate is regulated by both interactions between stem cells and interactions between stem cells and membrane proteins. ... Manipulating cell seeding density is a common biological technique in controlling cell-cell interactions, but controlling local ...
... contained in the central cell) in the centre of the gametophyte. The resulting cell is triploid (3n). This triploid cell ... In seed plants, after pollination, a pollen grain germinates, and a pollen tube grows and penetrates the ovule through a tiny ... Resact is a 14 amino acid peptide purified from the jelly coat of A. punctulata that attracts the migration of sperm. ... First, the cytoplasms of the two gamete cells fuse (called plasmogamy), producing a dikaryotic or heterokaryotic cell with ...
Morpholinos conjugated with cell penetrating peptides have been shown effective in protecting mice from influenza A.[15][16] ... Cell culture[edit]. Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering ... Cell culture (cell-based) manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines ... In contrast, cell culture manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines (e ...
The small size of the penicillins increases their potency, by allowing them to penetrate the entire depth of the cell wall. ... Molecular Biotechnology of Fungal beta-Lactam Antibiotics and Related Peptide Synthetases. Advances in Biochemical Engineering/ ... The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly subject to stress ... This weakens the cell wall of the bacterium, and osmotic pressure becomes increasingly uncompensated-eventually causing cell ...
Mitral cells also project to periglomerular cells and granular cells that inhibit the mitral cells surrounding it (lateral ... Boehm T, Zufall F (February 2006). "MHC peptides and the sensory evaluation of genotype". Trends in Neurosciences. 29 (2): 100- ... Odorants penetrate into the cuticle pores of chemosensory sensilla and get in contact with insect odorant-binding proteins ( ... A large degree of convergence occurs, with 25,000 axons synapsing on 25 or so mitral cells, and with each of these mitral cells ...
The accelerated particles penetrate both the cell wall and membranes. The DNA separates from the metal and is integrated into ... end of the gene to a chloroplast transit peptide derived from the petunia EPSPS. This transit peptide was used because it had ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... Koornneef, M.; Meinke, D. (2010). "The development of Arabidopsis as a model plant". The Plant journal : for cell and molecular ...
Unlike chemical or physical triggers for cancer, penetrating radiation hits molecules within cells randomly.[note 1] Molecules ... Effect of Ionizing Radiation on the Formation of Age-Correlated Oligo Deoxyribo Nucleo Phospheryl Peptides in Mammalian Cells; ... Cancer starts with a single cell whose operation is disrupted. Normal cell operation is controlled by the chemical structure of ... Aug 2000). "Therapeutic ionizing radiation and the incidence of basal cell carcinoma and squamous cell carcinoma. The New ...
... wavelength of 890 nm is able to penetrate into the subcutaneous tissue where it acts upon a specialized part of the cell called ... Elevated levels of glucose within cells cause a non-enzymatic covalent bonding with proteins, which alters their structure and ... The nitric oxide in turn promotes vasodilation which results in increased blood flow that helps nourish damaged nerve cells. ... the cytochrome C. The infrared light energy prompts the cytochrome C to release nitric oxide into the cells. ...
Calcitonin gene related peptides (CGRPs) have been found to play a role in the pathogenesis of the pain associated with ... a practice where needles are placed randomly or do not penetrate the skin.[95] Both have a possibility of being similar in ... including activation of NMDA receptors leading to calcium entering the cell.[72] After the burst of activity, the blood flow to ...
The electrospun scaffolds made for tissue engineering applications can be penetrated with cells to treat or replace biological ... "A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular ... This early application of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types ... These fibers may then be coated with collagen to promote cell attachment, although collagen has successfully been spun directly ...
"A Noncovalent Peptide-Based Strategy for Peptide and Short Interfering RNA Delivery". Handbook of Cell-Penetrating Peptides, ... prediction of cell-penetrating peptides Bioinformatics (2013) 29 (23): 3094-3096... *CellPPDMod: Prediction of cell-penetrating ... Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake/uptake of various molecular equipment (from ... "A New Potent Secondary Amphipathic Cell-penetrating Peptide for siRNA Delivery into Mammalian Cells". Molecular Therapy. 17 (1 ...
CPP, Cell-Penetrating Peptides. Authors. * Ülo Langel Copyright. 2019. Publisher. Springer Singapore. Copyright Holder. ... Provides a comprehensive update of cell penetrating peptides. *Summarizes the CPP uptake mechanisms and their critical analysis ...
In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating ... a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas ... In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating ... Cell-Penetrating Penta-Peptides and Bax-Inhibiting Peptides: Protocol for Their Application ...
The presence of cell and tissue barriers together with the low biomembrane permeability of various therapeutics often hampers ... Cell-Penetrating Peptides: From Basic Research to Clinics Trends Pharmacol Sci. 2017 Apr;38(4):406-424. doi: 10.1016/j.tips. ... Cell-Penetrating Peptides / classification * Cell-Penetrating Peptides / pharmacokinetics * Cell-Penetrating Peptides / ... increase medicament concentrations in areas that are difficult to access now exist with the advent of cell-penetrating peptides ...
In Cell-Penetrating Peptides: Methods and Protocols, e ... a new understanding of cell-penetrating peptides has emerged, ... In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating ... Cell-Penetrating Penta-Peptides and Bax-Inhibiting Peptides: Protocol for Their Application ... Enhanced Cellular Delivery of Cell-Penetrating Peptide-Peptide Nucleic Acid Conjugates by Photochemical Internalization ...
Researchers suggest that the peptide constructs act as general amyloid inhibitors, with potential applications in many amyloid- ... Home Neurological Disorders Alzheimers Alzheimers Disease Protein Aggregation Prevented by Cell-Penetrating Peptides ... NYUAD researchers have developed small proteins called cell-penetrating peptides (CPPs) that prevent the aggregation of the Aβ ... "Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity.". Alzheimers disease, Huntingtons ...
... the wealth of new information on the use of these peptides as transport systems has ... Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, ... Classes and Prediction of Cell-Penetrating Peptides, Classes and Prediction of Cell-Penetrating Peptides, Functionality of ... Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these ...
Cell-penetrating peptides are short and basic peptides are widely used due to their ability to deliver a cargo across the ... However, the crossing of cellular membranes constitutes the principal impediment to gaining entry into cells, and the potential ... In this wide-ranging scenario, a fascinating novel hypothesis is that membranotropic peptides that efficiently cross biological ... This review summarizes current data on membranotropic peptides for drug delivery. ...
In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an ... preventing the nuclear import of pSMAD3 using the TAT-SNX9 peptide inhibited profibrotic TGF-β activity in murine cells and ... TGF-β signaling mediated by pSMAD2, bone morphogenetic protein 4 (BMP4), EGF, or PDGF was unaffected by the TAT-SNX9 peptide. ... Furthermore, while the TAT-SNX9 peptide prevented TGF-βs profibrotic activity in vitro as well as in 2 murine treatment models ...
In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an ... AKR-2B cells were transduced for 90 minutes with the indicated concentration of TAT peptide. Following washing and 1 hour TGF-β ... Cell lysate reflects signal obtained from 10 μg total protein; the slower migrating band in the pSMAD3 lane is a nonspecific ... Left panels: AKR-2B cells were incubated with vehicle (top panels) or transduced (bottom panels) as in B. with TAT-SH3 or TAT- ...
Cell-penetrating peptides (CPP) can translocate across the cell membrane and have been extensively studied for the delivery of ... proteins, nucleic acids, and therapeutics in mammalian cells. However, characterizations of CPP in plants have only recently ... Cell-penetrating peptides (CPP) can translocate across the cell membrane and have been extensively studied for the delivery of ... Furthermore, a 22-residue peptide derived from the N-terminal region of the CaP (CPNT) can enter barley protoplasts and cells ...
Development of cell-penetrating peptide-modified MPEG-PCL diblock copolymeric nanoparticles for systemic gene delivery. Int J ... biodistribution brain tumors cell penetrating peptide intranasal brain delivery micelles Takanori Kanazawa and Hiroyuki Taki ... Cell-Penetrating Peptide-Modified Block Copolymer Micelles Promote Direct Brain Delivery via Intranasal Administration. ... In addition, cellular uptake of coumarin by rat glioma cells transfected with coumarin-loaded MPEG-PCL or MPEG-PCL-Tat was ...
Dendritic analogues of cell penetrating peptides, with multiple guanidine groups on their peripheries offer advantages as their ... and comparisons of their efficacies with those of known cell penetrating peptides. The toxicities of these dendritic guanidines ... This review describes the development of cell-penetrating dendrimers based on several different backbones, their structure- ... The widespread application of cell penetrating agents to clinical therapeutics and imaging agents relies on the ability to ...
Cell penetrating peptides (CPPs) are short-chain peptides developed as functionalized vectors for delivery approaches of ... Here a functional peptide (CPP,i,ecp,/i,) has been identified from critical HS binding region in hRNase3, a unique RNase family ... On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to ... significant inhibitory effects on cancer cell migration, and suppression of angiogenesis,i, in vitro,/i, and,i, in vivo,/i,. ...
Cell-penetrating peptides (CPPs) uptake mechanism is still in need of more clarification to have a better understanding of ... Role of autophagy in cell-penetrating peptide transfection model.. [Moataz Dowaidar, Maxime Gestin, Carmine Pasquale Cerrato, ... in HeLa cells, have been investigated. The RNA expression profile was characterized by RNA sequencing and confirmed by qPCR ...
However, only a few examples are known to penetrate the fungal cell wall. We compared the capacity of different fluorophore- ... An increasing number of peptides translocate the plasma membrane of mammalian cells promising new avenues for drug delivery. ... Free uptake of cell-penetrating peptides by fission yeast FEBS Lett. 2005 Aug 29;579(21):4873-8. doi: 10.1016/j.febslet.2005.07 ... Most of the 20 peptides tested were able to enter human cells, but only one, transportan 10 (TP10), efficiently penetrated ...
... among them are cell-penetrating peptides (CPPs). CPPs (a.k.a., PTDs) are proteins that are capable of penetrating cell ... Cell viability in mammalian cells. In this study, human bronchoalveolar carcinoma A549 cells were used as a model cell line to ... A Review of Hypotoxic Fluorescent Nanoparticles Delivery by Cell-Penetrating Peptides in Multiple Organisms. By Creative ... And the good news is that the particles did not produce lethal effects in mammalian cells, archaea, rotifers, Gram-positive ...
The peptides exhibiting these properties, now known as cell-penetrating peptides (CPP) [3], "Trojan peptides" or protein ... Cell-penetrating peptides for nanomedicine - how to choose the right peptide. Ilja Tabujew*Max Planck Institute for Polymer ... In the past, cell penetrating peptides were often classified as protein derived, model peptides or designed CPPs [6], however, ... More than two decades ago, a group of peptides, now known as cell-penetrating peptides, sparked the hope that the ultimate ...
"A Noncovalent Peptide-Based Strategy for Peptide and Short Interfering RNA Delivery". Handbook of Cell-Penetrating Peptides, ... Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake and uptake of molecules ranging from ... Meade BR, Dowdy SF (March 2007). "Exogenous siRNA delivery using peptide transduction domains/cell penetrating peptides". ... "A new potent secondary amphipathic cell-penetrating peptide for siRNA delivery into mammalian cells". Molecular Therapy. 17 (1 ...
Numerous types of drugs have been transported into cells using CPPs, including small-molecule pharmaceuticals, therapeutic ... have found numerous applications in biology and medicine since the first synthetic cell-permeable sequence was identified two ... Cell-penetrating peptides. (CPPs) have found numerous applications in biology and medicine since the first synthetic cell- ... Cell-penetrating peptides. as delivery vehicles for biology and medicine Kelly M. Stewart,b Kristin L. Hortonb and Shana O. ...
Jungwirth from IOCB Prague has discovered a previously unknown mechanism by which short peptides are able to penetrate cells ... Its known that peptides can also penetrate cells passively, i.e. independently of energy from the cell, but the exact ... How do peptides penetrate cells? Two sides of the same coin Institute of Organic Chemistry and Biochemistry of the Czech ... How do peptides penetrate cells? Two sides of the same coin. Institute of Organic Chemistry and Biochemistry of the Czech ...
A cell-penetrating ARF peptide inhibitor of FoxM1 in mouse hepatocellular carcinoma treatment. ... A cell-penetrating ARF peptide inhibitor of FoxM1 in mouse hepatocellular carcinoma treatment. ... we subjected these HCC-bearing mice to daily injections of a cell-penetrating ARF26-44 peptide inhibitor of FoxM1 function. ... ARF peptide treatment also induced apoptosis of several distinct human hepatoma cell lines, which correlated with reduced ...
... into a multi-branched topology with short dipeptide branches gave cell penetrating peptide dendrimers (CPPDs) with higher cell ... Designed cell penetrating peptide dendrimers efficiently internalize cargo into cells† Gabriela A. Eggimann,‡a Emilyne Blattes, ... Designed cell penetrating peptide dendrimers efficiently internalize cargo into cells G. A. Eggimann, E. Blattes, S. Buschor, R ... Redesigning linear cell penetrating peptides (CPPs) into a multi-branched topology with short dipeptide branches gave cell ...
The uptake mechanisms by which cell-penetrating peptides enter cells depend on the conditions, such as the cell line the ... Cell-penetrating peptides with the ability to escape endosomes and reach the target are of great value as delivery vectors for ... The ability of cell-penetrating peptides to cross plasma membranes has been explored for various applications, including the ... Oligonucleotide Complexes with Cell-Penetrating Peptides: Structure, Binding, Translocation and Flux in Lipid Membranes. ...
Buy or Rent Cell-Penetrating Peptides as an eTextbook and get instant access. With VitalSource, you can save up to 80% compared ... Cell-Penetrating Peptides 1st Edition by Ulo Langel and Publisher CRC Press. Save up to 80% by choosing the eTextbook option ...
Cell-penetrating peptides (CPPs), also known as protein transduction domains, are a class of diverse peptides with 5‑30 amino ... Jones AT and Sayers EJ: Cell entry of cell penetrating peptides: tales of tails wagging dogs. J Control Release. 161:582-591. ... Vivès E, Schmidt J and Pèlegrin A: Cell-penetrating and cell-targeting peptides in drug delivery. Biochim Biophys Acta. 1786: ... Jones AT: Macropinocytosis: Searching for an endocytic identity and role in the uptake of cell penetrating peptides. J Cell Mol ...
Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of ... Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of ... Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of ... Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of ...
... loaded novel cell-penetrating peptide (CPP)-modified pH-sensitive liposomes (CPPL) (referred to as CPPL(DOX)) with an optimal ... Compared to PEGylated liposomes, CPPL enhanced DOX accumulation in tumors up to 1.9-fold (p,0.01) and resulted in more cell ... but large cell dissolving areas were found in tumors following the treatment of animals with CPPL(DOX). Our findings provide ... In vivo study of doxorubicin-loaded cell-penetrating peptide-modified pH-sensitive liposomes: biocompatibility, bio- ...
... and sequence of 732 chemically modified cell-penetrating peptides and an equal number of non-cell penetrating peptides. We ... and sequence of 732 chemically modified cell penetrating peptides and an equal number of non-cell penetrating peptides. We ... and residues are preferred in cell-penetrating peptides. In this study, models were developed to predict cell-penetrating ... and residues are preferred in cell-penetrating peptides. In this study, models were developed to predict cell penetration ...
Cell-penetrating peptides are excluded from the mitochondrial matrix M.F. Ross M.F. Ross 1 ... Cell-penetrating peptides do not cross mitochondrial membranes even when conjugated to a lipophilic cation: evidence against ... M.F. Ross, M.P. Murphy; Cell-penetrating peptides are excluded from the mitochondrial matrix. Biochem Soc Trans 1 November 2004 ... CPPs (cell-penetrating peptides) facilitate cellular uptake of covalently attached macromolecules, through an as yet ...
  • Cell-penetrating peptides ( CPPs ) are short peptides that facilitate cellular intake/uptake of various molecular equipment (from nanosize particles to small chemical molecules and large fragments of DNA). (wikipedia.org)
  • The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to delivery vectors for use in research and medicine. (wikipedia.org)
  • A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net charge or have hydrophobic amino acid groups that are crucial for cellular uptake. (wikipedia.org)
  • A recent discovery found that Papillomaviridae such as the Human Papillomavirus use CPPs to penetrate the intracellular membrane in order to trigger retrograde trafficking of the viral unit to the nucleus. (wikipedia.org)
  • Cell-penetrating peptides are of different sizes, amino acid sequences, and charges but all CPPs have one distinct characteristic, which is the ability to translocate the plasma membrane and facilitate the delivery of various molecular cargoes to the cytoplasm or an organelle. (wikipedia.org)
  • In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. (springer.com)
  • Prof. Ulo Langel … rightly explain in a necessary preface that there are in use so many definition for the same things: protein transduction domain (PTDs), Trojan peptides, model amphipathic peptides (MAPs), membrane translocating sequences (MTS) that the best way to refer to all of these molecules is to call all of them cell-penetrating peptides, CPPs. (springer.com)
  • Opportunities to increase medicament concentrations in areas that are difficult to access now exist with the advent of cell-penetrating peptides (CPPs), which can transport into the cell a wide variety of biologically active conjugates (cargoes). (nih.gov)
  • NYUAD researchers have developed small proteins called cell-penetrating peptides (CPPs) that prevent the aggregation of the Aβ protein. (genengnews.com)
  • An international research team headed by scientists at New York University (NYU) Abu Dhabi has developed small proteins called cell-penetrating peptides (CPPs), which can prevent formation of the amyloid-β (Aβ) protein aggregates that are characteristic of Alzheimer's disease (AD), and so inhibit Aβ-induced neurotoxicity. (genengnews.com)
  • This has necessitated the use of drug-delivery systems, such as CPPs, in order to overcome major physiological obstacles, e.g., the blood-brain and blood-cerebrospinal fluid barriers, and facilitate efficient delivery of the amyloid inhibitor peptides. (genengnews.com)
  • CPPs are short peptides, typically 5-40 residues, the authors explained. (genengnews.com)
  • CPPs alone, or coupled to cargoes many times their own molecular mass, enter cells with high efficiency and low toxicity in vitro and in vivo and target specific intracellular organelles. (genengnews.com)
  • Cell penetrating peptides (CPPs) are short-chain peptides developed as functionalized vectors for delivery approaches of impermeable agents. (hindawi.com)
  • On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to multiple cellular effects. (hindawi.com)
  • Cell penetrating peptides (CPPs) are short-chain cationic and/or amphipathic peptides which may be internalized into living cells [ 7 ]. (hindawi.com)
  • Most CPPs are rich in positively charged Arg and Lys residues and are internalized after initially interacting with cell surface negatively charged GAGs which cluster CPPs on outer membrane surfaces [ 9 , 10 ]. (hindawi.com)
  • Cell-penetrating peptides (CPPs) uptake mechanism is still in need of more clarification to have a better understanding of their action in the mediation of oligonucleotide transfection. (sigmaaldrich.com)
  • To circumvent this issue, several strategies have been developed, among them are cell-penetrating peptides (CPPs). (selfgrowth.com)
  • CPPs deliver the cargo into cells, commonly through endocytosis, for use in research and medicine. (wikipedia.org)
  • Cell-penetrating peptides (CPPs) have found numerous applications in biology and medicine since the first synthetic cell-permeable sequence was identified two decades ago. (rsc.org)
  • Numerous types of drugs have been transported into cells using CPPs, including small-molecule pharmaceuticals , therapeutic proteins , and antisense oligonucleotides . (rsc.org)
  • Improved agents for medical imaging have been generated by conjugation with CPPs, with the appended peptides promoting cellular uptake and in some cases, cell-type specificity. (rsc.org)
  • Redesigning linear cell penetrating peptides (CPPs) into a multi-branched topology with short dipeptide branches gave cell penetrating peptide dendrimers (CPPDs) with higher cell penetration, lower toxicity and hemolysis and higher serum stability than linear CPPs. (rsc.org)
  • Cell-penetrating peptides (CPPs), also known as protein transduction domains, are a class of diverse peptides with 5‑30 amino acids. (spandidos-publications.com)
  • CPPs (cell-penetrating peptides) facilitate cellular uptake of covalently attached macromolecules, through an as yet controversial mechanism that either involves direct membrane passage or a type of endocytosis. (portlandpress.com)
  • We investigated the potential of the CPPs penetratin and Tat to act as mitochondria-targeting vectors by testing whether they were internalized by isolated mitochondria, and by mitochondria within cells in culture. (portlandpress.com)
  • Our results validate a promising first step in achieving control over cell penetration and to use CPPs for therapeutic approaches. (rsc.org)
  • Cell-penetrating peptides (CPPs) have the ability to penetrate the otherwise impermeable plasma membrane. (rsc.org)
  • 1-5 Although the use of CPP-cargo constructs for cellular delivery is promising, CPPs are hardly cell-type specific, and there is no temporal control over their uptake. (rsc.org)
  • 6-8 The use of CPPs for therapeutic purposes would greatly benefit if cell-type selectivity and control over their moment of uptake could be induced. (rsc.org)
  • Whereas the truncated split peptides did not induce uptake in HeLa cells, the assembled octa- and nona-arginines were efficient CPPs. (rsc.org)
  • Our results show that peptide stability to membrane-bound, secreted or intracellular proteases varies between different CPPs and that the suitability of individual CPPs for a particular cargo in complex formation by non-covalent interactions requires detailed studies. (mdpi.com)
  • Cells vary in their sensitivity to increasing concentrations of CPPs. (mdpi.com)
  • Cell-penetrating peptides (CPPs) have been receiving much attention over the last few decades due to their unique ability to translocate across plasma membranes efficiently and carry the wide range of macromolecular cargoes with them. (springer.com)
  • Although their intracellular delivery mechanism has not been fully elucidated, CPP-based cellular uptake, mainly mediated by direct transduction or endocytosis, depends on structural characteristics of CPPs, the type and concentration of CPPs or cargoes, and cell types being treated. (springer.com)
  • To overcome the intrinsic drawbacks of CPPs including lack of cell or tissue specificity, short plasma half-life, and endosomal sequestration, various strategies have been suggested such as activatable CPPs (ACPPs), introduction of targeting moieties and fusion peptides, and topical delivery systems for clinical application. (springer.com)
  • In this review, we provide an overview of up-to-date knowledge of intracellular delivery mechanisms of CPPs and introduce potential topical applications of CPPs such as transdermal, nasal and ocular delivery for non-invasive delivering the therapeutic proteins or peptides. (springer.com)
  • The use of CPPs (cell-penetrating peptides) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. (portlandpress.com)
  • Since then, several CPPs, including the widely used Tat (transactivator of transcription) peptide, have been developed and utilized to translocate a wide range of compounds across the plasma membrane of cells both in vivo and in vitro . (portlandpress.com)
  • Therefore we selected three of the major CPPs, penetratin, Tat and transportan 10, and evaluated their ability to deliver commonly used cargos, including fluoresceinyl moiety, double-stranded DNA and proteins (i.e. avidin and streptavidin), and studied their effect on membrane integrity and cell viability. (portlandpress.com)
  • Furthermore, and no less importantly, the toxicity of CPPs depends heavily on peptide concentration, cargo molecule and coupling strategy. (portlandpress.com)
  • No proper definition of CPPs has been formulated, but most, if not all, carry a net positive charge, are less than 30 amino acids long and have the ability to rapidly translocate large molecules into cells. (portlandpress.com)
  • Originally it was believed that CPPs translocated across cell membranes in a receptor- and energy-independent manner. (portlandpress.com)
  • However, more recent studies suggest that the uptake for most CPPs is an energy-dependent process with initial binding of peptides to proteoglycans on the cell surface and concomitant endocytosis of peptides [ 6 - 8 ]. (portlandpress.com)
  • Cell-penetrating peptides (CPPs) have proven their potential as an efficient delivery system due to their intrinsic ability to traverse biological membranes and transport various cargoes into the cells. (ovid.com)
  • There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. (springer.com)
  • Taken together, our data highlight an innovative approach to develop novel peptide-based DNA repair inhibitors and establish BRCA2-derived CPPs as promising anticancer agents. (aacrjournals.org)
  • Cell penetrating peptides (CPPs) may improve efficient delivery of a wide range of macromolecular cargos including plasmid DNA, small interfering RNA, drugs, nanoparticulate pharmaceutical carriers and anticancer drugs. (aspetjournals.org)
  • Examples of successful application of CPPs in treatment of human diseases are also presented, including decreased tumor growth and induction of cancer cell death. (aspetjournals.org)
  • Noncovalent complexation of plasmid DNA (pDNA) using cell penetrating peptides (CPPs) has been less explored due to the relatively large complex size formed and the low-level gene expression. (ku.edu)
  • POPC) phospholipid monolayers at the air-water interface are used as model cell membranes to monitor the membrane insertion potential of synthetic CPPs. (ku.edu)
  • Cell-penetrating peptides (CPPs) have been widely used to deliver nanomaterials and other types of macromolecules into mammalian cells for therapeutic and diagnostic use. (sciencemag.org)
  • NRP1 binds peptides carrying a positive residue at the carboxyl terminus, a feature that is compatible with cationic CPPs, either intact or after proteolytic processing. (sciencemag.org)
  • Cationic cell-penetrating peptides (CPPs) have been widely exploited to overcome this barrier and deliver various types of cargo into mammalian cells ( 1 ). (sciencemag.org)
  • The recent interest in cationic cell penetrating peptide (CPPs) stems from their potential utility as non-invasive drug delivery platforms. (usc.edu)
  • By conjugating CPPs to a wide range of impermeable cargo, their intracellular delivery has shown to be increased in different types of cells by a less defined mechanism of transduction. (usc.edu)
  • Considering that the original protein from which the cationic CPPs are derived, HIV-1 Tat protein, is able to exit the infected cell and internalize into neighbouring cells, it is conceivable that CPPs can enter into differentiated epithelial cells from the apical domain and exit on the basolateral domain. (usc.edu)
  • This hypothesis was tested by investigating the transport of CPPs in alveolar epithelial cells and also by conjugating CPPs to a peptide and a protein drug intended for oral and pulmonary delivery respectively. (usc.edu)
  • The results presented in this thesis show that CPPs can be transported across alveolar epithelial cells without severely compromising cellular integrity. (usc.edu)
  • Cell-penetrating peptides (CPPs) have been proven as a promising gene delivery tool. (physiciansweekly.com)
  • Since their discovery twenty years ago, cell-penetrating peptides (CPPs) or protein transduction domains (PTDs) have been described as promising drug delivery systems. (ens.fr)
  • Along with the development of CPPs/PTDs as vectors to carry various macromolecules for targeted cellular therapies, engineering new CPPs/PTDs with optimized transport and internalization capacities would greatly benefit from the understanding of cell entry mechanisms that still remain subject of controversy in the field7. (ens.fr)
  • The study herein directly addresses the issue of the quantification and the mechanisms of cell entry, by providing reliable and robust methods to detect total CPPs/PTDs inside cells. (ens.fr)
  • Indeed, CPP internalization and subsequent intracellular traffic are highly dynamic processes, and quantitative snapshots of CPPs/PTDs internalization in restricted areas of the cells, might only provide an incomplete picture of CPP functionality. (ens.fr)
  • Irrespective of the nature of the tag added to track their fate, most CPPs strongly interact with the cell surface generating a pool of membrane-bound peptides usually defined as high membrane- or cell surface binding peptides, which cannot be eliminated by saline washings12-14. (ens.fr)
  • The quantities of internalized CPPs measured by fluorometry in cell lysates converge with those obtained by our previously reported mass spectrometry quantification method. (ens.fr)
  • We investigated the metabolic stability of four cell penetrating peptides (CPPs), namely SAP, hCT(9-32)-br, [Palpha] and [Pbeta], when in contact with either subconfluent HeLa, confluent MDCK or Calu-3 epithelial cell cultures. (uzh.ch)
  • Cell penetrating peptides (CPPs) possessing powerful transmembrane capacity have been shown to be effective transport vectors for bioactive molecules and an attractive alternative to traditional active targeting approaches. (eurekaselect.com)
  • To evaluate the efficacy of anti-VEGF agents for treating choroidal neovascularization (CNV) when delivered topically using novel cell-penetrating peptides (CPPs) compared with delivery by intravitreal (ivit) injection. (arvojournals.org)
  • CPPs are nontoxic to ocular cells and can be used to deliver therapeutically relevant doses of ranibizumab and bevacizumab by eye drop to the posterior segment of mouse, rat, and pig eyes. (arvojournals.org)
  • Cell-penetrating peptides (CPPs) are efficient vehicles to transport bioactive molecules into the cells. (diva-portal.org)
  • In order to decrease the dependence upon bacterial vectors and increase transformation efficiency, we have researched an alternative method for introducing nucleic acids into plants that does not involve Agrobacterium and instead uses cell penetrating peptides (CPPs). (escholarship.org)
  • Most surprisingly plant cell walls can also be bypassed, as CPPs are currently used in plants in transient expression and gene silencing assays. (escholarship.org)
  • Cell-penetrating peptides (CPPs) belong to a class of delivery vectors that have been extensively used for the cellular delivery of various, otherwise impermeable, macromolecules. (ox.ac.uk)
  • Here, for the first time, the cellular uptake kinetics of eight well-established CPPs is compared in HeLa pLuc 705 cells using a recently published releasable luciferin assay. (ox.ac.uk)
  • Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs) or membrane transduction peptides (MTPs), are small molecular peptides that consist of 5-30 amino acid residues, which not only penetrate the cell membrane by themselves, but also carry a variety of exogenous substances such as peptides, proteins, nucleic acids and nanoparticles in a covalent or non-covalent binding manner into cells. (creative-peptides.com)
  • CPPs have a wide range of transmembrane targets and can enter a variety of cell types, even through the blood-brain barrier when applied in vivo . (creative-peptides.com)
  • As an effective bioactive molecular intracellular transport tool, CPPs have broad application prospects in cell biology and cellular immunology, especially in the fields of drug development, gene biotherapy and tumor targeted therapy. (creative-peptides.com)
  • Intracellular and especially cytosolic delivery with cell penetrating peptides (CPPs) can be severely limited by endocytotic uptake, leading to unwanted vesicular entrapment. (mpg.de)
  • A correlation between cell-surface thiols and uptake efficiency of disulfilde-containing CPPs was discussed and it was shown that these CPPs possess much better cytosolic targeting capability [1].Our newly developed, cysteine-rich CPP, CyLoP-1 (Cytosol Localizing Peptide 1, CRWRWKCCKK) showed pronounced cytosolic delivery already in its non-oxidized form. (mpg.de)
  • In this book, expert researchers explore the latest information on cell-penetrating peptides (CPPs). (jamalon.com)
  • Cell Penetrating Peptides (CPPs, also known as Cell Permeable Peptides or as Protein Transduction Domains, PTDs), are carriers with small peptide domains (generally less than 40 amino acids) that can easily cross cell membranes. (novoprolabs.com)
  • However, cell-penetrating peptides (CPPs) have the ability to enter the plasma membrane of a cell independent of a membrane receptor. (novoprolabs.com)
  • CPPs are capable of delivering biologically active cargo to the cell interior. (novoprolabs.com)
  • Cell Penetrating Peptides (CPPs), also called transduction peptides, are short peptides that can carry macromolecules into cells. (jenkemusa.com)
  • Although CPPs can deliver different types of substances into cells, their practical application has focused on the cellular transport of oligopeptides, proteins, and nucleic acids. (jenkemusa.com)
  • The disadvantage of using CPPs is that a large number of cationic penetrating peptides may cause side effects due to non-specific interactions with negatively charged molecules in cells or negatively charged extracellular molecules into cells. (jenkemusa.com)
  • Cell- penetrating peptides (CPPs) have attracted scientists' attention in recent decades, because they can overcome the hydrophobic plasma membrane barrier and enter cells directly. (mst.edu)
  • Cell-penetrating peptides (CPPs), including TAT-CPP, have been used to deliver exogenous proteins into living cells. (elsevier.com)
  • Studies of CPPs (cell-penetrating peptides), sequences that are also commonly designated as protein transduction domains, now extend to a second decade of exciting and far-reaching discoveries. (openrepository.com)
  • CPPs are proven vehicles for the intracellular delivery of macromolecules that include oligonucleotides, peptides and proteins, low-molecular-mass drugs, nanoparticles and liposomes. (openrepository.com)
  • Cationic cell-penetrating peptides have been widely used to enhance the intracellular delivery of various types of cargoes, such as drugs and proteins. (nih.gov)
  • Intracellular Delivery of Short Interfering RNA in Rat Organ of Corti Using a Cell-penetrating Peptide PepFect6. (nih.gov)
  • Cell penetrating peptides have gained much recognition as a versatile transport vehicle for the intracellular delivery of wide range of cargoes ( i.e. oligonucelotides, small molecules, proteins, etc. ), that otherwise lack bioavailability, thus offering great potential as future therapeutics. (biomedcentral.com)
  • Cell-penetrating peptides (CPP) have attracted many scientists' attention as intracellular delivery tools due to their high cargo molecule transportation efficiency and low cytotoxicity. (physiciansweekly.com)
  • Two cell entry mechanisms cooperate in peptide-mediated intracellular delivery. (sciencemag.org)
  • Protein transduction domains (PTDs) are powerful nongenetic tools that allow intracellular delivery of conjugated cargoes to modify cell behavior. (nottingham.ac.uk)
  • These innovative products, developed in collaboration with Tianjin Medical University, are useful for intracellular delivery of RNA and DNA oligonucleotide and other molecules that cannot penetrate the cell membrane. (jenkemusa.com)
  • Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides (2004) Gupta Bhawna et al. (naver.com)
  • Development of cell-penetrating peptide-modified MPEG-PCL diblock copolymeric nanoparticles for systemic gene delivery. (springer.com)
  • In this study, human bronchoalveolar carcinoma A549 cells were used as a model cell line to investigate CPP-mediated uptake of inorganic fluorescent nanoparticles. (selfgrowth.com)
  • Collectively, semiconductor fluorescent nanoparticles and their CPP-modified complexes did not reduce cell viability. (selfgrowth.com)
  • Additionally, we provide an overview of the conjugation techniques usually employed for the attachment of cell penetrating peptides to quantum dots, as well as silver and gold nanoparticles, and we address the various aspects that need to be considered for the successful implementation of cell penetrating peptides for organelle-specific delivery of nanoparticles into cells. (degruyter.com)
  • We highlight the recent applications of cell penetrating peptides in the targeted delivery of quantum dots as well as gold, silver and polymeric nanoparticles into mammalian cells in the context of organelle specificity. (degruyter.com)
  • We give a short overview of the conjugation techniques usually utilized for the attachment of cell penetrating peptides to quantum dots as well as silver and gold nanoparticles. (degruyter.com)
  • Lewin M, Carlesso N, Tung CH, Tang XW, Cory D, Scadden DT and Weissleder R: Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. (spandidos-publications.com)
  • Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases. (nih.gov)
  • We now report in vivo visualization of matrix metalloproteinase activities by MRI and fluorescence of dendrimeric nanoparticles coated with activatable cell penetrating peptides (ACPPs), labeled with Cy5, gadolinium, or both. (nih.gov)
  • Barua S, Mitragotri S (2014) Challenges associated with penetration of nanoparticles across cell and tissue barriers: a review of current status and future prospects. (springer.com)
  • PF6/siRNA nanoparticles lead to knockdown of target genes, a housekeeping gene and supporting cell-specific connexin 26. (nih.gov)
  • Methods: Here, we used CADY, a secondary amphipathic peptide of 20 residues that could successfully carry siRNA into mammalian cells, to prepare CADY/ASOs nanoparticles (CADY-NPs) targeting acpP (encoding acyl carrier protein), and evaluated the uptake features, the inhibitory effects of CADY-NPs on gene expression and the growth of MDR-A. baumannii. (eurekaselect.com)
  • A large number of different cargo molecules such as oligonucleotides, peptides, peptide nucleic acids, proteins or even nanoparticles have been internalized in cells by this strategy. (cnrs.fr)
  • The transfection efficacy of siRNA containing lipid-like nanoparticles decorated with different peptides was evaluated both in vitro and in vivo and correlated with the peptide physical and chemical properties. (mysciencework.com)
  • In vivo, peptide decorated nanoparticles primarily accumulated in the lungs and the liver. (mysciencework.com)
  • Although they have been used successfully for carrying different cargoes of varying size and nature (plasmid DNA, peptides, proteins, nanoparticles, and quantum dots, etc.), the most rapid progress has been made with the delivery of oligonucleotides (ONs). (novoprolabs.com)
  • Plant organelle targeting cell penetrating peptides (POTCPPs) are peptides that form nanoparticles composed of short peptides and double-stranded DNA (dsDNA). (uleth.ca)
  • However, the crossing of cellular membranes constitutes the principal impediment to gaining entry into cells, and the potential therapeutic application of many drugs is predominantly dependent on the development of delivery tools that should take the drug to target cells selectively and efficiently with only minimal toxicity. (mdpi.com)
  • We showed that the intact virion and a recombinant capsid protein (CaP) from a plant-infecting nonenveloped icosahedral RNA virus, Brome mosaic virus (BMV), can penetrate the membranes of plant protoplasts but are trapped by the extracellular matrix. (apsnet.org)
  • PTDs) are proteins that are capable of penetrating cell membranes. (selfgrowth.com)
  • A better understanding of the basic processes occurring within the membranes of the cells in our bodies gradually opens the door to new possibilities for controlling these processes and thereby conceivably more efficient methods of transporting drug molecules to their site of action. (eurekalert.org)
  • The ability of cell-penetrating peptides to cross plasma membranes has been explored for various applications, including the delivery of bioactive molecules to inhibit disease-causing cellular processes. (diva-portal.org)
  • We conclude that the transport vectors involved in this study are efficient at perturbing lipid membranes, which correlates with their remarkable capacity to transport oligonucleotides into cells. (diva-portal.org)
  • Deshayes S, Plénat T, Aldrian-Herrada G, Divita G, Le Grimellec C and Heitz F: Primary amphipathic cell-penetrating peptides: Structural requirements and interactions with model membranes. (spandidos-publications.com)
  • Cell-penetrating peptides are able to transport a wide variety of cargo across cell membranes. (rsc.org)
  • Fatemeh Madani, Alex Perálvarez-Marín, and Astrid Gräslund, "Liposome Model Systems to Study the Endosomal Escape of Cell-Penetrating Peptides: Transport across Phospholipid Membranes Induced by a Proton Gradient," Journal of Drug Delivery , vol. 2011, Article ID 897592, 7 pages, 2011. (hindawi.com)
  • peptides, which are able to cross cellular membranes and reach biological targets even when administered systemically, represents a promising tool to overcome this issue. (drugdiscoverytoday.com)
  • The insertion potential of complexes having different cationic (dTAT, H9, K9, R9, and RH9) and amphiphilic (RA9, RL9, and RW9) peptides were recorded using a Langmuir monolayer approach that records complexes adsorption to model membranes. (ku.edu)
  • Cell membranes are a great obstacle for entrance of gene therapeutic agents. (physiciansweekly.com)
  • In particular, one should understand how these peptides cross cell membranes. (ens.fr)
  • One of the major problems in cell biology is the inefficient transfer of peptides or proteins across cellular membranes. (novoprolabs.com)
  • Cell penetrating sequences can be used as extensions to peptide sequences thereby making them more permeable to cell membranes, or cell penetrating peptides can be attached to other cargo molecules to enhance their cellular uptake. (novoprolabs.com)
  • Cell-penetrating peptide CPP: guides the TIVA tag through cell membranes into tissues. (wikipedia.org)
  • The constructs effectively combine beneficial properties of proteins with potent therapeutic effects and highly efficient delivery to target cells. (genengnews.com)
  • These studies indicate that ARF peptide treatment is an effective therapeutic approach to limit proliferation and induce apoptosis of liver cancer cells in vivo. (jci.org)
  • These peptides have a significant potential for diagnostic and therapeutic applications, such as delivery of fluorescent or radioactive compounds for imaging, delivery of peptides and proteins for therapeutic application, and delivery of molecules into induced pluripotent stem cells for directing differentiation. (spandidos-publications.com)
  • Zahid M and Robbins PD: Cell-type specific penetrating peptides: Therapeutic promises and challenges. (spandidos-publications.com)
  • Although promising, they are not often considered for therapeutic purposes as they lack controllable activity and cell selectivity. (rsc.org)
  • Keeping in mind the therapeutic importance of these peptides, we have developed in silico methods for the prediction of cell penetrating peptides, which can be used for rapid screening of such peptides prior to their synthesis. (biomedcentral.com)
  • Researchers at UC San Diego have demonstrated the therapeutic potential of utilizing cell penetrating peptides and antibody drug conjugates (ADC) to deliver radiosensitizers. (universityofcalifornia.edu)
  • The mechanism of cell-penetrating peptides entry into cells is unclear, preventing the development of more efficient vectors for biotechnological or therapeutic purposes. (ens.fr)
  • Cell penetrating peptides have demonstrated potential to facilitate the cellular delivery of therapeutic molecules. (mysciencework.com)
  • This recombinant polyvalent vaccine peptide, toolbox101, is-depending on the route of administration-specifically immunosuppressive and can be used to vaccinate against experimental autoimmune encephalomyelitis, neuritis, and uveoretinitis and is, in addition, of therapeutic value. (cell-penetrating-peptides.com)
  • Introducing therapeutic genetic material into cells is key for gene therapy. (jenkemusa.com)
  • It is on excessive light modes from epub cell penetrating peptides methods and protocols data and guys to ia and Things and to more international Humanities, bulk as policy, the security, borders, ultimately significantly as therapeutic other Wars. (skaal.com)
  • Aguilera TA, Olson ES, Timmers MM, Jiang T, Tsien RY (2009) Systemic in vivo distribution of activatable cell penetrating peptides is superior to that of cell penetrating peptides. (springer.com)
  • Activatable cell penetrating peptides (ACPPs) are novel in vivo targeting agents comprised of a polycationic cell penetrating peptide (CPP) connected via a cleavable linker to a neutralizing polyanion ( Fig. 1A ). (pubmedcentralcanada.ca)
  • To overcome this obstacle, activatable cell penetrating peptides (ACPPs) target various cargoes to sites of protease activity in vivo. (universityofcalifornia.edu)
  • We discuss the mechanisms of cell penetrating peptide's uptake and briefly introduce the different types of peptides in order to present a more complete picture of the intracellular fate of the peptide-cargo conjugates. (degruyter.com)
  • In this study, we designed and synthesized the conjugates TAT-CPT and TAT-2CPT by attaching camptothecin (CPT) to the N-terminus of the cell penetrating peptide TAT. (ovid.com)
  • In this study, we examined the molecular mechanism of photochemical internalization of cell penetrating peptide-cargo (macromolecule)-photosensitizer conjugates. (researchmap.jp)
  • Antibody drug conjugates (ADC) were synthesized and the conjugated antibodies were validated in vitro on tumor xenographs which radiosensitized tumor cells. (universityofcalifornia.edu)
  • Cellular internalization kinetics of (luciferin-)cell-penetrating peptide conjugates. (ox.ac.uk)
  • However, cellular uptake of free oligomers is poor and the utility of conjugates of PNA or PMO to cell penetrating peptides (CPP), such as Tat or Penetratin, is limited by endosomal sequestration. (naver.com)
  • In the past, number of in silico methods have been developed for predicting cell-penetrating property of peptides containing natural residues. (frontiersin.org)
  • In this study, first time attempt has been made to predict cell-penetrating property of peptides containing natural and modified residues. (frontiersin.org)
  • We analyzed the structure of both class of peptides and observed that positive charge groups, atoms, and residues are preferred in cell-penetrating peptides. (frontiersin.org)
  • 16,17 For these peptides it is known that a minimum amount of six arginine residues is needed to induce cellular uptake. (rsc.org)
  • These peptides are short sequences consisting mostly of arginine or lysine residues including the Tat peptide, Antp peptide and different oligomers of arginine and lysine. (usc.edu)
  • Specially, peptide 1, which contained three Api(C2Gu) residues, formed a stable helical structure and passed through the cell membrane more efficiently than the other peptides. (physiciansweekly.com)
  • They are usually small peptides 10-30 residues in length with positively charged amino acid sequences. (novoprolabs.com)
  • This webserver has been designed to assist the scientific community to predict the cell penetrating potential of modified cell penetrating peptide having natural and chemically modified amino acid residues. (edu.in)
  • This module allows users to predict the cell penetrating potential of a peptide containing chemically modified residues. (edu.in)
  • In addition, cellular uptake of coumarin by rat glioma cells transfected with coumarin-loaded MPEG-PCL or MPEG-PCL-Tat was determined. (springer.com)
  • Cellular uptake of self-assembled cationic peptide-DNA complex: multifunctional role of the enhancer chloroquine. (springer.com)
  • CPP-mediated cellular uptake can be found from prokaryotic to eukaryotic organisms including mammalian cells, aquatic microorganism, yeasts, insect cells, mice dermis, plant tissue, Gram-negative and Gram-positive bacteria, and archaea. (selfgrowth.com)
  • Oehlke J, Scheller A, Wiesner B, Krause E, Beyermann M, Klauschenz E, Melzig M and Bienert M: Cellular uptake of an alpha-helical amphipathic model peptide with the potential to deliver polar compounds into the cell interior non-endocytically. (spandidos-publications.com)
  • Efficient noncytotoxic cellular uptake of a nona-arginine (R9)-conjugated version of the BRC4 peptide interferes with DNA damage-induced RAD51 foci formation and HR. Moreover, transduction of the BRC4 peptide impairs replication fork-protective function of BRCA2 and triggers MRE11-dependent degradation of nascent DNA in response to DNA replication stress. (aacrjournals.org)
  • It has been found that HIV-1 TAT and its core peptide segment TAT47-57 play an important role in promoting the cellular uptake of coupled bioactive macromolecules, such as peptides, proteins, oligonucleotides, and drug molecules. (deepdyve.com)
  • However, the mechanism behind the cellular uptake of HIV-1 TAT-derived cell-penetrating peptide remains unclear. (deepdyve.com)
  • Multiple cell permeable peptides have been identified that facilitate cellular uptake of various molecular cargo, ranging from nanosize particles to small chemical molecules. (novoprolabs.com)
  • Cell-penetrating peptides (CPP) are highly cationic short peptides that have the ability to promote the receptor-independent cellular uptake of macromolecules such as peptides and nanoparticules. (sb-peptide.com)
  • In collaboration with colleagues from the Czech Republic and Germany, the research team of Pavel Jungwirth from the Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) has discovered a previously unknown mechanism by which short peptides are able to penetrate cells and, in principle, could serve as carriers of drug molecules. (eurekalert.org)
  • The ability of positively charged short peptides to penetrate cells was first observed in HIV research, and today it's gradually being employed to transport drugs into cells. (eurekalert.org)
  • Cell-penetrating peptides (CPP) can translocate across the cell membrane and have been extensively studied for the delivery of proteins, nucleic acids, and therapeutics in mammalian cells. (apsnet.org)
  • These findings extend the knowledge of the variables that condition intracellular Cell-penetrating peptide mediated transport of nucleic acids, which ultimately translates into a small step towards successful non-viral gene therapy. (diva-portal.org)
  • Gene therapy is an approach that introduces nucleic acids into cells and aims to correct the gene function by altering the gene expression to prevent, halt, or reverse a pathological process (1). (teknoscienze.com)
  • We determined that a 10-nucleotide oligomer of peptide nucleic acid (PNA) targeting the start codon region of an essential gene acpP is able to cause complete growth inhibition of E. amylovora . (frontiersin.org)
  • Moreover, the electrostatic interaction between siRNA and transmembrane peptides is a disadvantage for siRNA-based nucleic acid drug delivery. (jenkemusa.com)
  • Introducing a polyethylene glycol flexible segment between CPP and the nucleic acid prevents avoids the electrostatic interaction between the nucleic acid drug and the membrane-penetrating peptide which causes aggregation and sedimentation. (jenkemusa.com)
  • This in turn allows the nucleic acid drug to exert its biological activity in the cytoplasm and enhance its bioavailability after entering the cell. (jenkemusa.com)
  • The molecular weight of polyethylene glycol can be adjusted according to the specific type of nucleic acid drug delivered, the specific type of transmembrane peptide and the difference of the charge between the two. (jenkemusa.com)
  • In Paper I, the cytotoxic and immunogenic activities of PepFect peptides and PepFect/nucleic acid complexes were analyzed in vitro and in vivo. (ut.ee)
  • The current study confirmed that PepFect peptides possess promising potential in nucleic acid delivery without evidence of undesired cytotoxicity and inflammation at the concentrations of 10 μM and 5 μM, respectively in vitro and at a dose of 5 mg/kg in vivo. (ut.ee)
  • In Paper IV, we showed that N-terminal modification of non-amphipathic (RxR)4 with stearic acid moiety resulted in efficient peptide vector for nucleic acids delivery in non-toxic manner. (ut.ee)
  • Charge neutral steric block oligonucleotide analogues, such as peptide nucleic acids (PNA) or phosphorodiamidate morpholino oligomers (PMO), have promising biological and pharmacological properties for antisense applications, such as for example in mRNA splicing redirection. (naver.com)
  • Stability of peptide nucleic acids in human serum and cellular extracts (1994) Demidov Vadim V et al. (naver.com)
  • Abnormal expression of cell surface molecules notably contributes to enhance tumor cell growth, survival, migration, and invasiveness [ 4 ]. (hindawi.com)
  • More than two decades ago, a group of peptides, now known as cell-penetrating peptides, sparked the hope that the ultimate carrier molecules have been found. (degruyter.com)
  • The peptides exhibiting these properties, now known as cell-penetrating peptides (CPP) [3], "Trojan peptides" or protein transduction domains (PTD), sparked hope that the ultimate carrier molecules for the transport of small molecules and nanocarriers have been found. (degruyter.com)
  • If, however, this newly discovered mechanism proves sufficiently robust, in the future we could consider the possibility of passively transporting drug molecules into cells without having to free them from vesicles, which in this process simply don't form. (eurekalert.org)
  • Efficient delivery of heterologous molecules for treatment of cells is a great challenge in modern medicine and pharmacology. (aspetjournals.org)
  • However, the early TAT peptide derived from the HIV transcription activator protein has been proven that the sequence contains Furin protease cleaved motifs which limited the TAT application in delivery of exogenous active molecules. (physiciansweekly.com)
  • Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. (syntides.com)
  • Trojan peptides or cell-penetrating peptides (CPP) are natural or designed peptides identified as cellular membrane-crossing molecules, in particular through their potency to vehiculate various kinds of compounds to the cytoplasm and nucleus of living cells. (syntides.com)
  • Therefore, Cell-penetrating peptides can be used as drug delivery systems to transport a cargo molecules into cells. (sb-peptide.com)
  • Proteins and peptides represent promising classes of therapeutics for many types of diseases because they are biocompatible, biodegradable, and can selectively bind to specific targets, which reduces the potential for toxicity, the researchers noted. (genengnews.com)
  • We determined that specifically preventing the nuclear import of pSMAD3 using the TAT-SNX9 peptide inhibited profibrotic TGF-β activity in murine cells and human lung fibroblasts as well as in vivo with no demonstrable toxicity. (jci.org)
  • Tunnemann G, Ter-Avetisyan G, Martin RM, Stockl M, Herrmann A and Cardoso MC: Live-cell analysis of cell penetration ability and toxicity of oligo-arginines. (spandidos-publications.com)
  • Khafagy E-S, Kamei N, Nielsen EJB, Nishio R, Takeda-Morishita M (2013) One-month subchronic toxicity study of cell-penetrating peptides for insulin nasal delivery in rats. (springer.com)
  • CPP toxicity was investigated in cell cultures. (arvojournals.org)
  • In conclusion, the current work provides evidence for the development of apoptotic protein-derived cell-penetrating peptides and tumor-targeted cell-penetrating peptide-based drug delivery vector with reduced toxicity and immunogenicity. (ut.ee)
  • rationally designed peptides, which include sequences derived from the target amyloid protein, and randomly generated peptides, which are often identified from library screens," the investigators noted. (genengnews.com)
  • Ragin AD, Morgan RA and Chmielewski J: Cellular import mediated by nuclear localization signal Peptide sequences. (spandidos-publications.com)
  • The investigators reported on their developments and results in Cell Reports Physical Science , in a paper titled, " Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity . (genengnews.com)
  • Properties and behavior of adhesion and suspension vertebrate cells as well as the protozoa Leishmania tarentolae are investigated with respect to proteolytic activity, uptake efficiency, intracellular localization and cytotoxicity. (mdpi.com)
  • Laser scanning confocal microscopy and flow cytometry confirmed that seven out of eight peptides were internalized into live cells with varying efficiencies without significant cytotoxicity. (ovid.com)
  • We show that PF6 was internalized by all cells without inducing cytotoxicity in cochlear cultures. (nih.gov)
  • After enediyne-energized with chromophore of lidamycin, the energized fusion protein dFv-LDP-AE and dFv-R-LDP-AE showed potent cytotoxicity to PC cells and could induced the robust cell apoptosis and necrosis in vitro . (jcancer.org)
  • Cell-penetrating peptides are short and basic peptides are widely used due to their ability to deliver a cargo across the membrane both in vitro and in vivo . (mdpi.com)
  • In addition to cellular binding and internalization, CPP ecp demonstrated multiple functions including strong binding activity to tumor cell surface with higher HS expression, significant inhibitory effects on cancer cell migration, and suppression of angiogenesis in vitro and in vivo . (hindawi.com)
  • Alterations of cell surface receptor, coreceptor, and adhesive protein expression are reported in various cancer types in vitro and in vivo [ 1 - 3 ]. (hindawi.com)
  • An ACPP cleavable by matrix metalloproteinase-2 (MMP-2) in vitro was the first one demonstrated to work in a tumor model in vivo , but only HT-1080 xenografts and resected human squamous cell carcinomas were tested. (pubmedcentralcanada.ca)
  • Additionally, oligoarginine conjugated to protein drug, e.g., insulin, can facilitate the transport of insulin in vitro across alveolar epithelial cells by a transcellular pathway and can enhance insulin transport in vivo when delivered into the lungs of diabetic rats. (usc.edu)
  • Lidamycin (also named C-1027), an enediyne antibiotics, had shown potent antitumor efficacy in kinds of caner cell line in vitro and in vivo . (jcancer.org)
  • Three human peptides derived from surfactant protein B (a lung surfactant protein), orexin (a neuropeptide hormone, and lactoferricin (a globular glycoprotein) that exist in many physiological fluids facilitated the in vivo delivery of siRNA and induce significant knock down (90%) of a hepatocyte expressed protein, coagulation Factor VII. (mysciencework.com)
  • The human immunodeficiency virus-1 (HIV-1) Tat peptide is taken up by a variety of cells in vitro and in vivo (Anderson et al. (cell-penetrating-peptides.com)
  • In vivo near-infrared fluorescence imaging of integrin a2ß1 in prostate cancer with cell-penetrating-peptide-conjugated DGEA probe. (ucdenver.edu)
  • The POTCPP transfection method represents the first report of a successful in vivo plant cell mitochondrial transfection and a peptide based plastid transfection within a monocot species. (uleth.ca)
  • Naked siRNA has poor stability and is easily degraded by the nuclease in vivo, and its properties such as high negative charge, and hydrophilicity prevent its transport through the cell membrane. (jenkemusa.com)
  • A transcriptome in vivo analysis tag (TIVA tag) is a multifunctional, photoactivatable mRNA-capture molecule designed for isolating mRNA from a single cell in complex tissues. (wikipedia.org)
  • As translocation across the plasma membrane is a limiting step for delivery of macromolecules, these polypeptides were utilized in a pancreatic xenograft model to study the plasma clearance, biodistribution, tumor accumulation, and tumor reduction capabilities of the polypeptide with and without a cell-penetrating peptide. (dovepress.com)
  • Translocation efficiencies were determined using fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM). (uzh.ch)
  • Throughout, we observed higher translocation efficiencies into HeLa cells as compared to MDCK and Calu-3, corresponding to the lower state of differentiation of HeLa cell cultures. (uzh.ch)
  • Åmand HL, Rydberg HA, Fornander LH, Lincoln P, Nordén B, Esbjörner EK (2012) Cell surface binding and uptake of arginine-and lysine-rich penetratin peptides in absence and presence of proteoglycans. (springer.com)
  • We have found that the cell penetrating peptides (CPP) Tat or penetratin (Pen), fused with elastin-like polypeptide (CPP-ELP) inhibited adhesion, spreading, invasion and migration of SKOV-3 ovarian cancer cells, SK-MEL-2 melanoma cells, and MDA-MB-231 breast cancer cells in cell culture. (aacrjournals.org)
  • The uptake of the other peptides, pVec, penetratin, M918, and EB1, is much slower and is consistent with the reported observations of endocytosis being the predominant internalization mechanism. (ox.ac.uk)
  • As heparan sulfate proteoglycans (HSPGs) are known as co-receptors to interact with numerous growth factors and then modulate downstream biological activities, overexpression of HS/HSPG on cell surface acts as an increasingly reliable prognostic factor in tumor progression. (hindawi.com)
  • Interaction between cell surface and microenvironment plays a crucial role in malignant tumor progression. (hindawi.com)
  • With the advance of science and technology, nanomaterials have been seen in various biomedical applications, including molecular labeling and tracking, DNA/RNA/proteins probing, drug delivery and therapies via techniques of biocujugation , tumor or tissue targeting, peptide drug discovery , pathogenic intervention as well as biomedical imaging. (selfgrowth.com)
  • After 4 weeks of this treatment, HCC regions displayed reduced tumor cell proliferation and angiogenesis and a significant increase in apoptosis within the HCC region but not in the adjacent normal liver tissue. (jci.org)
  • 0.01) and resulted in more cell apoptosis as a result of DNA disruption as well as a relatively lower tumor growth ratio (T/C%). Histological examination did not show any signs of necrosis or inflammation in normal tissues, but large cell dissolving areas were found in tumors following the treatment of animals with CPPL(DOX). (dovepress.com)
  • The majority of chemotherapeutic agents used in clinics are highly toxic to both tumor cells and normal tissues due to the lack of specificity. (dovepress.com)
  • Macromolecules are gaining interest in drug delivery because they preferentially accumulate due to their ability to extravasate into the tumor much more readily compared to a normal cell well. (dovepress.com)
  • Tumor cell metastasis is a complex, multi-step process that is a major cause of morbidity and death amongst cancer patients. (aacrjournals.org)
  • Isolated tumor endothelial cells maintain specific character during long-term culture. (biomedsearch.com)
  • Once cleaved, they release drug-conjugated polycation cell penetrating peptides that are taken up by tumor cells. (universityofcalifornia.edu)
  • Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide. (ucdenver.edu)
  • In Paper III, a novel tumor-selective peptide-based drug delivery platform gHoPe2 has been developed demonstrating potential application of CPP-s for tumor-targeted delivery. (ut.ee)
  • Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore. (eurekalert.org)
  • Brock R (2014) The uptake of arginine-rich cell-penetrating peptides: putting the puzzle together. (springer.com)
  • First, using bioinformatics approach, arginine-rich peptide segments were extracted from SwissProt proteins and their cell-penetrating properties were predicted. (ovid.com)
  • Zhong G, Xu Z, Yang R, Zhang S, Li L, Wu M, Liu H, Zhen Y. An arginine-rich cell penetrating peptide contained anti-gelatinase scFv-LDM fusion protein shows potent antitumor efficacy in pancreatic cancer. (jcancer.org)
  • To improve the penetration capability, the fusion protein dFv-LDP-AE was integrated with an arginine-rich cell penetrating peptide (Arg) 9 and then generated the fusion protein dFv-R-LDP-AE. (jcancer.org)
  • Arginine-rich cell-penetrating peptides (CPP) are widely employed as delivery vehicles for a large variety of macromolecular cargos. (ru.nl)
  • This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP-cargo samples including high-throughput cell viability screening. (springer.com)
  • The current thesis is focused on the characterization of immunogenic and cytotoxic activities of cell-penetrating peptides PepFects, on the cytotoxic and apoptotic activities of newly designed cell-penetrating p53 analogues and on the development of a peptide-based glioma-targeted drug delivery vector. (ut.ee)
  • novel cell-penetrating peptides. (etis.ee)
  • In this study, models were developed to predict cell-penetrating peptides from its tertiary structure using a wide range of descriptors (2D, 3D descriptors, and fingerprints). (frontiersin.org)
  • The present study demonstrates that features like amino acid composition, binary profile of patterns and motifs, can be used to train an SVM classifier that can predict cell penetrating peptides with higher accuracy. (biomedcentral.com)
  • These peptides are known to form noncovalent, nano-sized complexes with diverse oligonucleotide cargoes. (diva-portal.org)
  • Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake, that is why other delivery mechanisms have been developed like CellSqueeze and electroporation . (wikipedia.org)
  • Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs. (springer.com)
  • The uptake mechanisms by which cell-penetrating peptides enter cells depend on the conditions, such as the cell line the concentration and the temperature. (diva-portal.org)
  • The general purpose of this thesis is to reveal the mechanisms by which our in house designed peptides enter cells and allow the successful transport of biofunctional oligonucleotide cargo. (diva-portal.org)
  • Examination of the antimicrobial mechanisms demonstrated that anti- acpP -CPP1 caused dose-dependent reduction of acpP mRNA in E. amylovora upon treatment and resulted in cell death (bactericidal effect). (frontiersin.org)
  • Here, we review the biology, classification and mechanisms of internalization of cell-penetrating peptides. (drugdiscoverytoday.com)
  • We also describe different CPP families with regard to their physicochemical properties and numerous mechanisms of CPP cell uptake by direct penetration and endocytotic pathways. (aspetjournals.org)
  • The mechanisms by which CPP enter cells remain unknown. (cnrs.fr)
  • In this review, we discuss the uptake mechanism and application of cell penetrating peptides as mediators for organelle specific delivery of nanocarriers, pointing out the possibilities as well as strategies of their successful utilization. (degruyter.com)
  • This mechanism explains how key ingredients, such as the cooperation among the peptides, the large positive charge, and specifically the guanidinium groups, contribute to the uptake. (wikipedia.org)
  • It's known that peptides can also penetrate cells passively, i.e. independently of energy from the cell, but the exact mechanism has yet to be described. (eurekalert.org)
  • Now, using fluorescence and electron microscopy in combination with molecular computer simulations, the scientists led by Pavel Jungwirth have discovered a previously undescribed passive mechanism for transporting positively charged peptides into cells. (eurekalert.org)
  • To be used as therapeutics, each novel cell-penetrating peptide needs to be fully characterized, including their physicochemical properties, their biological activity and their uptake mechanism. (diva-portal.org)
  • We describe the convergence of an HS-dependent pathway with the C-end rule (CendR) mechanism that enables peptide ligation with neuropilin-1 (NRP1), a cell surface receptor known to be involved in angiogenesis and vascular permeability. (sciencemag.org)
  • Taken together, attachment of CPT could turn cell penetrating peptide TAT into an antimicrobial peptide with a dual mechanism of anticancer action, which presents a new strategy to develop anticancer peptides based on cell penetrating peptides. (ovid.com)
  • Cell-Penetrating Peptides 1st Edition by Ulo Langel and Publisher CRC Press. (vitalsource.com)
  • Lindgren M, Hallbrink M, Prochiantz A and Langel U: Cell-penetrating peptides. (spandidos-publications.com)
  • Lindgren M, Rosenthal-Aizman K, Saar K, Eiríksdóttir E, Jiang Y, Sassian M, Ostlund P, Hällbrink M, Langel U (2006) Overcoming methotrexate resistance in breast cancer tumour cells by the use of a new cell-penetrating peptide. (springer.com)
  • Mai JC, Shen H, Watkins SC, Cheng T and Robbins PD: Efficiency of protein transduction is cell type-dependent and is enhanced by dextran sulfate. (spandidos-publications.com)
  • It is a peptide rich in basic amino acids and belongs to the protein transduction domain family. (deepdyve.com)
  • We report on different cell-penetrating peptides applied for vectorization of splice-correcting oligonucleotides using both covalent conjugation and non-covalent nanoparticle formation approach. (teknoscienze.com)
  • We found that conjugation of cell penetrating peptide (CPP) to PNA is essential for the antimicrobial effect, with CPP1 [(KFF)3K] being the most effective against E. amylovora . (frontiersin.org)
  • here drugs conjugation with cell penetrating peptides, in particular with HIV-derived Tat peptide is discussed. (drugdiscoverytoday.com)
  • A promising strategy to overcome this problem consists in the chemical conjugation (or fusion) to cell penetrating peptides (CPP) derived from proteins able to cross the plasma membrane. (cnrs.fr)
  • This conjugation will make your peptide cell permeable. (novoprolabs.com)
  • ACPPs consist of a polycationic cell penetrating peptide attached to a cargo and a polyanionic inhibitory domain with a protease cleavable linker. (universityofcalifornia.edu)
  • Dendritic analogues of cell penetrating peptides, with multiple guanidine groups on their peripheries offer advantages as their high symmetry allows them to be efficiently synthesized, while orthogonal functionalities at their focal points allow them to be conjugated to cargo using simple synthetic methods. (mdpi.com)
  • Jafari S, Maleki Dizaj S and Adibkia K: Cell-penetrating peptides and their analogues as novel nanocarriers for drug delivery. (spandidos-publications.com)
  • In Paper II, we generated short p53-derived cell-penetrating protein analogues and analyzed their internalization efficacy, cytotoxic and apoptotic activities in the neuroblastoma and breast cancer cell lines. (ut.ee)
  • Even though the effect was observed under serum-free conditions, the novel protein analogues demonstrated selective apoptotic activity in the p53-non-active breast cancer cell line suggesting promising perspectives for a future anticancer strategy. (ut.ee)
  • The "cargo" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions . (wikipedia.org)
  • In this study, the effect on early events (1 h treatment) in transfection by PepFect14 (PF14), with or without oligonucleotide cargo on gene expression, in HeLa cells, have been investigated. (sigmaaldrich.com)
  • Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake. (wikipedia.org)
  • The latter is often preferred because of easy handling, uptake efficiency and auto-release of cargo into the live cell. (mdpi.com)
  • For each cargo, cell type and CPP the optimal conditions must be determined separately. (mdpi.com)
  • Although the field has emerged as a possible future candidate for drug delivery, little attention has been given to the potential toxic side effects that these peptides might exhibit in cargo delivery. (portlandpress.com)
  • We also show at the ultrastructural level, using a novel cell entry synchronization method, that both the HS and NRP1 pathways can initiate a macropinocytosis-like process and visualize these CPP-cargo complexes going through various endosomal compartments. (sciencemag.org)
  • On the other hand, neuropilin-1 (NRP1) was recently suggested to play a role in the cell entry of TAT-bound cargo ( 8 ). (sciencemag.org)
  • If this is true, they can also ferry impermeable cargo across the epithelial cells, thus aid in overcoming one of the notorious barriers for oral and pulmonary delivery of proteins and peptides. (usc.edu)
  • Single particle tracking (SPT) allows following individual CPP/cargo complex through endosomal path in live cells, using fluoresently labled cargo and green fluoresent protein expressing cells. (diva-portal.org)
  • SmacN7-CyLoP-1 was capable of inducing apoptosis in HeLa cells, while SmacN7 alone had no effect, proving efficient delivery of the bioactive cargo into the cytosol. (mpg.de)
  • These properties enabled the peptides to translocate their cargo across the cell wall and outer plasma membrane of tissue cultured plant cells to deliver dsDNA to the mitochondria or the chloroplast and have it expressed in organello. (uleth.ca)
  • The BMV CPNT overlaps with the sequence known to bind BMV RNA, and it can deliver BMV RNAs into cells, resulting in viral replication, as well as deliver double-stranded RNAs that can induce gene silencing. (apsnet.org)
  • Finally, the capacity of CVP1-N2 for delivery of gene into cells was determined, where it was able to carry red fluorescent protein (RFP) and apoptin genes into cells respectively and induce the apoptosis. (physiciansweekly.com)
  • 3. CVP1-N2 was able to deliver the apoptin gene into HCT116 cells and induce apoptosis. (physiciansweekly.com)
  • When the peptides were presented to bone marrow-derived dendritic cells, they induce low immunoactivation relative to control cell penetrating peptides including the antennapedia homeodomain and TAT, as quantified by the expression of activation specific surface proteins like CD80, CD86, and major histocompatibility complex class II. (mysciencework.com)
  • In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an aminoterminal sequence of sorting nexin 9 (SNX9), which was previously shown to bind phosphorylated SMAD3 (pSMAD3). (jci.org)
  • The widespread application of cell penetrating agents to clinical therapeutics and imaging agents relies on the ability to prepare them on a large scale and to readily conjugate them to their cargos. (mdpi.com)
  • The final conjugate will effectively deliver the target peptide into cells and then release the therapeutics such as siRNA or your target peptide in the reducing environment. (novoprolabs.com)
  • In this study, we applied a conjugate of cell-penetrating peptides (LMWP) and insulin (INS-PEG-LMWP) for buccal delivery. (ku.dk)
  • The venom peptide maurocalcin (MCa) is atypical among toxins because of its ability to rapidly translocate into cells and potently activate the intracellular calcium channel type 1 ryanodine receptor (RyR1). (elsevier.com)
  • Here, we report that both HIV-1 TAT47-57 peptide and the Chariot reagent are micromolar inhibitors of furin activity in vitro. (nih.gov)
  • In addition, cyclic polyarginine peptides containing hydrophobic moieties which have been previously used as transfection reagents also exhibited potent furin inhibition in vitro and also inhibited intracellular convertases. (nih.gov)
  • Furthermore, while the TAT-SNX9 peptide prevented TGF-β's profibrotic activity in vitro as well as in 2 murine treatment models of pulmonary fibrosis, a 3-amino acid point mutant that was unable to bind pSMAD3 proved ineffective. (jci.org)
  • Here a functional peptide (CPP ecp ) has been identified from critical HS binding region in hRNase3, a unique RNase family member with in vitro antitumor activity. (hindawi.com)
  • However, conjugating oligoarginine to a peptide drug, desmopressin, improved in vitro transport marginally but did not have any beneficial effect when delivered orally. (usc.edu)
  • Here, we report that MCa Thr 26 belongs to a consensus PKA phosphorylation site and can be phosphorylated by PKA both in vitro and after cell penetration in cellulo. (elsevier.com)
  • Then, the ability of CVP1-N2 cell penetrating was detected using confocal imaging and flow cytometry. (physiciansweekly.com)
  • 2017. https://nursing.unboundmedicine.com/nursingcentral/view/Tabers-Dictionary/749435/all/cell_penetrating_peptide. (unboundmedicine.com)
  • They have numerous applications in medicine as drug delivery agents in the treatment of different diseases including cancer and virus inhibitors, as well as contrast agents for cell labeling. (wikipedia.org)
  • These reagents are chemically similar to the multi-basic peptides that are known to be potent proprotein convertase inhibitors. (nih.gov)
  • Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation of non-covalent complexes. (mdpi.com)
  • NF51 facilitates rapid internalization of complexes into the cells, prolongs their stay in early endosomes and promotes release to cytosol. (diva-portal.org)
  • Thus, it was possible to discriminate cell-penetrating peptides from non-cell penetrating peptides based on amino acid composition. (biomedcentral.com)
  • Flow cytometric analysis of the contributing factors for antimicrobial activity enhancement of cell-penetrating type peptides: case study on engineered apidaecins. (biomedsearch.com)
  • Contributing factors for the antimicrobial activity enhancement of N-terminally engineered mutants of cell-penetrating apidaecins were analyzed based on their cell-penetration efficiency. (biomedsearch.com)
  • The flow cytometric analysis of the engineered apidaecins labeled with carboxyfluorescein (FAM) revealed their enhanced cell-penetrating efficiencies into Escherichia coli that should be one of key factors causing the enhanced antimicrobial activity. (biomedsearch.com)
  • It is noteworthy that, for one mutant, the enhancement in antimicrobial activity (18-fold higher than wild type) was greater than that of cell penetration (5.9-fold), suggesting that the N-terminal mutation may reinforce both interaction with unidentified intracellular target(s) and cell-penetration efficiency. (biomedsearch.com)
  • Interestingly, we found that TAT-CPT and especially TAT-2CPT could kill cancer cells via membrane disruption, which is similar to antimicrobial peptides. (ovid.com)
  • The current study demonstrated that dFv-LDP and dFv-R-LDP had high affinity with the antigen gelatinases and PC cells, the integration of (Arg) 9 could increase the penetration rate of fusion protein in SW-1990 and PANC-1 cells. (jcancer.org)
  • Cell penetration: scope and limitations by the application of cell‐penetrating peptides. (creative-peptides.com)
  • Heparan sulfate mediates cell uptake of aB-crystallin fused to the glycoprotein C cell penetration peptide. (ucdenver.edu)
  • Cell penetration peptides for enhanced entry of aB-crystallin into lens cells. (ucdenver.edu)
  • Alzheimer's disease, Huntington's disease, Parkinson's disease, and prion diseases are among the broad range of incurable degenerative disorders that are associated with the misfolding of proteins or peptides into aggregate known as amyloids, the authors explained. (genengnews.com)
  • Novel Cell-Penetrating Drug Delivery System for siRNA , 2018 TIDES Poster presentation . (jenkemusa.com)
  • Here, we report a novel cell-penetrating peptide (CPP)-based nonviral approach for delivering siRNA into inner ear tissue using organotypic cultures as model system. (nih.gov)
  • Interestingly, application of PF6/connexin 26 siRNA exhibited knockdown of both connexin 26 and 30 mRNA and their absence led to impaired intercellular communication as demonstrated by reduced transfer of calcein among the PF6/connexin 26-siRNA-treated cells. (nih.gov)
  • The arrows indicate the internalized siRNA-biotin in the hair cells (HC). (nih.gov)
  • JenKem Technology introduces a novel product line of LMWP Cell Penetrating Peptides PEG derivatives capable to transport siRNA efficiently through the cell membrane and release siRNA into the cytoplasm. (jenkemusa.com)
  • In addition, the cleaved fragment of TAT-αB-crystallin was significantly reduced in cells transfected with MMP-1 siRNA. (elsevier.com)
  • The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. (wikipedia.org)
  • In 1988, two laboratories independently found that TAT could be efficiently taken up from the surrounding media by numerous cell types in culture. (wikipedia.org)
  • The resulting activated peptide is taken up as efficiently as the well-established cell-penetrating peptide octa-arginine. (rsc.org)
  • Thus, most cells can be efficiently transduced with peptides, proteins and nucleotides with intracellular concentrations in the low micromole range. (mdpi.com)
  • Among these three peptides, one peptide (P8), derived from voltage-dependent L-type calcium channel subunit alpha-1D, was able to accumulate inside in a variety of cell types very efficiently through a rapid dose-dependent process. (ovid.com)
  • Recombinant TAT-αB-crystallin was efficiently transduced into H9c2 cells. (elsevier.com)
  • Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway. (ucdenver.edu)
  • Morris MC, Depollier J, Mery J, Heitz F, Divita G (2001) A peptide carrier for the delivery of biologically active proteins into mammalian cells. (springer.com)
  • 2016. "Quality Control of Cationic Cell-penetrating Peptides. (ugent.be)