Phenomenon of cell-mediated immunity measured by in vitro inhibition of the migration or phagocytosis of antigen-stimulated LEUKOCYTES or MACROPHAGES. Specific CELL MIGRATION ASSAYS have been developed to estimate levels of migration inhibitory factors, immune reactivity against tumor-associated antigens, and immunosuppressive effects of infectious microorganisms.
Protein factor(s) released by sensitized lymphocytes (and possibly other cells) that inhibit the movement of LEUKOCYTES, especially polymorphonuclear cells, away from their site of release. Assays for these factors are used as tests for cellular immunity. Two of the common assays are the LEUKOCYTE MIGRATION CAPILLARY TUBE TECHNIQUE (LMCT) and the LEUKOCYTE MIGRATION AGAROSE TEST (LMAT).
Proteins released by sensitized LYMPHOCYTES and possibly other cells that inhibit the migration of MACROPHAGES away from the release site. The structure and chemical properties may vary with the species and type of releasing cell.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
A protein extracted from boiled culture of tubercle bacilli (MYCOBACTERIUM TUBERCULOSIS). It is used in the tuberculin skin test (TUBERCULIN TEST) for the diagnosis of tuberculosis infection in asymptomatic persons.
Assays that measure the rate of migration of LEUKOCYTES. They may involve a variety of techniques such as measuring the movement of leukocytes through substrates such as AGAROSE gels or the rate of exit of cells from a glass capillary.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
Factor derived from leukocyte lysates of immune donors which can transfer both local and systemic cellular immunity to nonimmune recipients.
Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.
Substances that are recognized by the immune system and induce an immune reaction.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Prolamins in the endosperm of SEEDS from the Triticeae tribe which includes species of WHEAT; BARLEY; and RYE.
The serous fluid of ASCITES, the accumulation of fluids in the PERITONEAL CAVITY.
An active immunizing agent and a viable avirulent attenuated strain of Mycobacterium tuberculosis, var. bovis, which confers immunity to mycobacterial infections. It is used also in immunotherapy of neoplasms due to its stimulation of antibodies and non-specific immunity.
One of several skin tests to determine past or present tuberculosis infection. A purified protein derivative of the tubercle bacilli, called tuberculin, is introduced into the skin by scratch, puncture, or interdermal injection.
Test for cell-mediated antitumor immunity and related serum blocking factors based on the finding that leukocytes from cancer patients, but not from controls, when mixed in vitro with antigenic extracts of tumors of the same histological type, undergo a diminution in their normal adherence to glass surfaces. Sera from tumor-bearing patients block the LAI reaction of their own leukocytes or those of other patients with the same type of tumor.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Specific assays that measure the migration of cells. They are commonly used to measure the migration of immune cells in response to stimuli and the inhibition of immune cell migration by immunosuppressive factors.
An abundant cytosolic protein that plays a critical role in the structure of multilamellar myelin. Myelin basic protein binds to the cytosolic sides of myelin cell membranes and causes a tight adhesion between opposing cell membranes.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A malabsorption syndrome that is precipitated by the ingestion of foods containing GLUTEN, such as wheat, rye, and barley. It is characterized by INFLAMMATION of the SMALL INTESTINE, loss of MICROVILLI structure, failed INTESTINAL ABSORPTION, and MALNUTRITION.
Adherence of cells to surfaces or to other cells.
Substances of fungal origin that have antigenic activity.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Substances elaborated by bacteria that have antigenic activity.
A series of steps taken in order to conduct research.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
A cell line derived from cultured tumor cells.
An encapsulated lymphatic organ through which venous blood filters.
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
Periodic movements of animals in response to seasonal changes or reproductive instinct. Hormonal changes are the trigger in at least some animals. Most migrations are made for reasons of climatic change, feeding, or breeding.
Established cell cultures that have the potential to propagate indefinitely.
The lack of sufficient energy or protein to meet the body's metabolic demands, as a result of either an inadequate dietary intake of protein, intake of poor quality dietary protein, increased demands due to disease, or increased nutrient losses.
Peptides that have the ability to enter cells by crossing the plasma membrane directly, or through uptake by the endocytotic pathway.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.

Hypersensitivity pneumonitis: experimental production in calves with antigens of Micropolyspora faeni. (1/850)

Pneumonitis was induced in calves by exposure to aerosols of Micropolyspora faeni with or without prior sensitization of the animals by subcutaneous injection of antigen. The pneumonitis primarily involved centrolobular areas and was characterized by alveolar septal thickening and loss of air space by cellular infiltration. Vasculitis and focal haemorrhage occurred in certain individuals and haemoproteinaceous exudate appeared within septa and alveolar lumina. The pneumonitis was compared with human farmer's lung, pneumonitis of housed cattle and other experimental hypersensitivity pneumonitides.  (+info)

Lymphocyte proliferation inhibitory factor (PIF) in alcoholic liver disease. (2/850)

Lymphocyte proliferation inhibitory factor (PIF) was determined in the supernatants of PHA-stimulated lymphocytes from patients with alcoholic liver disease. PIF was assayed by determining inhibition of DNA synthesis in WI-38 human lung fibroblasts. A two-fold greater inhibition in thymidine incorporation into DNA by lung fibroblasts was observed in supernatants of PHA stimulated lymphocytes from patients with alcoholic hepatitis or active Laennec's cirrhosis as compared with that found in control subjects or patients with fatty liver. It is suggested that decreased liver cell regeneration seen in some patients with alcoholic hepatitis may be due to increased elaboration of PIF.  (+info)

Macrophage electrophoretic mobility (MEM) with myelin basic protein. (3/850)

Lymphocytes from a total of 161 subjects, including normal controls and patients with malignant and non-malignant conditions, have been investigated for their response to myelin basic protein, using the macrophage electrophoretic mobility (MEM) test. It has been confirmed that there was a high level of association between clinically evident cancer and a positive response. Lymphocytes from 24/25 patients with non-malignant inflammatory and ischaemic diseases also gave positive responses. In 46 patients with breast lumps studied before mastectomy or biopsy, the test was positive in 15/19 cases which proved to be malignant and in 5/27 which proved benign on histological examination. In its present form the test is not sufficiently reliable for the diagnosis of early cancer. Our results suggest that tissue necrosis in malignant and non-malignant conditions may be one of the factors resulting in sensitization to antigenic determinants present in preparations of myelin basic protein. Despite its technical difficulties, the test may provide a means of examing some aspects of immune recall not readily revealed by other test systems.  (+info)

Leucocyte migration inhibition with inner and outer membranes of mitochondria and insoluble hepatocyte surface membranes prepared from rat liver in patients with chronic hepatitis and cirrhosis. (4/850)

Patients with chronic liver disease were tested for delayed hypersensitivity to the outer and the inner membranes of mitochondria (OMM and IMM) and the insoluble hepatocyte-surface membranes (IHSM), prepared from rat livers, by means of leucocyte migration inhibition technique. Positive reaction to OMM was found in 37% of patients with chronic persistent hepatitis and 35% of those with chronic active hepatitis and 43% of those with liver cirrhosis (P less than 0-05). That to IMM was 55%, 43% and 36% (P less than 0-05) and to IHSM was 37%, 47% and 45% respectively (P less than 0-05). IHSM was found to contain liver-specific components and patients with positive response to IHSM did not reveal at all a positive reaction to rat renal cell-surface membranes. The incidence of positive response to IHSM was significantly higher (54-2%) in patients with the present or previous infection with HBAg than in HBAg-non-infected patients (21-4%) (P less than 0-05). And there seemed to be a good correlation between a degree of cellular response to purified HBsAg and that to IHSM in these HBAg-infected patients. No correlation, however, was found between that to purified HBsAg and that to OMM or IMM in the same patients. This suggested that the cellular response to either HBsAg or IHSM, both related closely, may play a role in the perpetuation of chronic liver disease.  (+info)

Cell-mediated immune responses in owl monkeys (Aotus trivirgatus) with trachoma to soluble antigens of Chlamydia trachomatis. (5/850)

The first temporal study of the cell-mediated immune responses (CMI) following ocular infections with Chlamydia trachomatis is presented. We examined the CMI of owl monkeys infected with trachoma to soluble antigens of C. trachomatis by leucocyte migration inhibition (LIF) and delayed hypersensitivity skin testing. Delayed hypersensitivity of a systemic nature developed after a local eye infection in owl monkeys; clearance of inclusions from conjunctival cells coincided with the onset of this response. The association of eye secretion and circulating antibodies with recovery from primary infection was not so striking. Both cellular and humoral immune responses persisted for at least 2 months, at which time all test animals were completely resistant to re-infection. The elicitation of cell-mediated immune reactions with solubilized chlamydial antigens may permit the isolation of specific antigens involved in the generation of protective immunity in the owl monkey model.  (+info)

Acquired cellular resistance, delayed hypersensitivity, and altered macrophage migration in Listeria monocytogenes-infected guinea pigs. (6/850)

A Listeria monocytogenes infection in guinea pigs was used to study the interrelationship between antigen-induced macrophage migration inhibition, delayed-type hypersensitivity, and acquired cellular resistance. Early after infection (at 2 and 7 days), very significant enhancement of macrophage migration was observed. Migration inhibition was detected beginning on day 14 and was uniformly observed only on day 21 of the infection, after which a shift again to enhancement was seen. The early detection (by day 2) of migration enhancement suggested that this assay may be more sensitive than assessment of delayed type hypersensitivity in vivo, which in this system was first detectable only on day 4. Acquired cellular resistance, as measured by enhanced survival following a high dose challenge with Listeria, was present from day 7 after infection until at least day 60. By splenic clearance studies, however, acquired cellular resistance was present only until day 14 after infection, suggesting that in this system splenic clearance was not a very reliable criterion for measuring acquired cellular resistance.  (+info)

Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins. (7/850)

Migration of neutrophils requires sequential adhesive and deadhesive interactions between beta 1 and beta 2 integrins and components of the extracellular matrix. Prompted by reports that describe interaction of soluble beta-glucan with the beta 2 integrin Mac-1, a role for beta-glucan in regulation of integrin-mediated migration was investigated. Neutrophil migration in response to fMLP was assessed using an agarose overlay method with slides precoated with fibronectin (Fn) +/- beta-glucan. On Fn, random migration in excess of directed migration was observed. In contrast, migration on Fn + beta-glucan was directional, with marked diminution of random migration. This conversion of random to directed migration was seen neither when Fn was supplemented with alternative polysaccharides nor when beta-glucan was applied to other components of the extracellular matrix. This effect of beta-glucan was shown to be cation dependent and to be effected by Arg-Gly-Asp-containing peptides consistent with an integrin-mediated event. mAb inhibition studies demonstrate that beta-glucan effects this shift toward directed migration through suppression of migration mediated by Mac-1 and very late Ag 5 and enhancement of very late Ag 3-mediated migration. Adhesion assays suggest that the prochemotactic influence of beta-glucan is due, in part but not entirely, to modulation of PMN adhesion to Fn. In summary, these data support a novel role for beta-glucan in regulation of beta 1- and beta 2-mediated neutrophil migration on Fn.  (+info)

Inhibition of tyrosine kinase activation blocks the down-regulation of CXC chemokine receptor 4 by HIV-1 gp120 in CD4+ T cells. (8/850)

Because the binding of HIV-1 envelope to CD4 initiates a configurational change in glycoprotein 120 (gp120), enabling it to interact with fusion coreceptors, we investigated how this process interferes with the expression and function of CXC chemokine receptor 4 (CXCR4) in CD4+ T lymphocytes. A recombinant gp120 (MN), after preincubation with CD4+ T lymphocytes, significantly inhibited the binding and chemotaxis of the cells in response to the CXCR4 ligand stromal cell-derived factor-1alpha (SDF-1alpha), accompanied by a markedly reduced surface expression of CXCR4. gp120, but not SDF-1alpha, induced rapid tyrosine phosphorylation of src-like kinase p56lck in CD4+ T cells, whereas both gp120 and SDF-1alpha caused phosphorylation of the CXCR4. The tyrosine kinase inhibitor herbimycin A abolished the phosphorylation of p56lck and CXCR4 induced by gp120 in association with maintenance of normal expression of cell surface CXCR4 and a migratory response to SDF-1alpha. Thus, a CD4-associated signaling molecule(s) including p56lck is activated by gp120 and is required for the down-regulation of CXCR4.  (+info)

Definition of Macrophage migration inhibition test with photos and pictures, translations, sample usage, and additional links for more information.
In seven patients with chronic beryllium disease (Be) the Be lymphocyte transformation test was positive in 100%, independent of steroid therapy, and was reproducible. The Be macrophage migration inhibition test was only positive in four of seven patients (57%) not on steroids, and was not reproducible. In 72 potentially exposed healthy beryllium workers the lymphocyte transformation test was negative in all subjects. The macrophage test was positive in four of 78 and again the results were not reproducible. The workers with positive results showed no differences in age, type or duration of employment from those with negative results and showed no evidence of disease. In addition, the macrophage test was positive in two of 45 non-exposed control subjects. We also confirmed the above advantages of the lymphocyte transformation technique by using tuberculin antigen (PPD). The PPD lymphocyte transformation test gave positive results in approximately 60% of healthy beryllium workers, but the PPD ...
Semantic Scholar extracted view of Immune responsiveness to Mycobacterium leprae of healthy humans. Application of the leucocyte migration inhibition test. by Bjørn Myrvang
Yamamoto, K and Anacker, R L., Macrophage migration inhibition studies with cells from mice vaccin- ated with cell walls of mycobacterium bovis bcg. Characterization of the experimental system. (1970). Subject Strain Bibliography 1970. 938 ...
Mowat, A M. and Ferguson, A, Migration inhibition of lymph node lymphocytes as an assay for regional cell-mediated immunity in the intestinal lymphoid tissues of mice immunized orally with ovalbumin. (1982). Subject Strain Bibliography 1982. 3434 ...
Semantic Scholar extracted view of Influence of route and dose of antigen on the migration inhibition and plaque-forming cell responses to sheep erythrocytes in the lizard, Calotes versicolor. by Swaminathan Jayaraman et al.
Cellular hypersensitivity to an extract of human pancreas, using the leucocyte migration test (LMT), was found in twenty-nine of 101 diabetic and eight of fifty normal control subjects. However, the difference in response between diabetics and controls was confined to young insulin-dependent patients, there being no distinction between normal subjects and older diabetics treated by diet or oral hypoglycemic agents. The use of rat liver mitochondria and bovine insulin as antigens in the LMT did not induce inhibition of leucocyte migration in diabetics or controls.. ...
Migration of monocytic-phagocytic cells from guinea pigs immunized with complete Freunds adjuvant is regularly and specifically inhibited in tissue culture by 5 to 10µg/ml of PPD. Migration of leukocytes and spindle cells from such animals is inhibited by PPD less consistently and to a lesser degree.. Cutaneous hypersensitivity to PPD in these animals develops 5 days after adjuvant immunization; at this time migration of mononuclear cells from explants of lung and regional node is inhibited by addition of 10 µg/ml of PPD. Splenic cells become sensitive 2 to 3 weeks after immunization and hepatic macrophages become sensitive only several weeks later.. Cellular hypersensitivity to antigen, as measured by this technique, develops in guinea pigs infected with Histoplasma or immunized with emulsions of complete Freunds adjuvant and T4 bacteriophage, bovine γ-globulin, human γ-globulin, or picrylated guinea pig skin. The sensitivity is specific for the immunizing antigen.. Serum from immunized ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint. ...
Wheat gluten derived antigens have been tested for their ability to inhibit the migration of leucocytes from healthy subjects and patients with coeliac disease. Three preparations of a water soluble fraction (Frazers fraction III, FIII) of partial peptic tryptic digests of wheat gluten had different effects in a direct (one stage) assay. Subfractions B and B2 caused migration inhibition of leucocytes from patients with treated coeliac disease but not of leucocytes from healthy volunteers or patients with Crohns disease or ulcerative colitis. This migration inhibition seems to be specific for gluten fractions because maize zein fraction B, beta-lactoglobulin and ovalbumin did not cause it. The sensitivity of coeliac leucocytes to fraction B is not related to factors present in coeliac serum as the migration of leucocytes from healthy individuals preincubated with coeliac sera was not inhibited. Puromycin diminished inhibition by fraction B, which was active at 1.2 micrograms/ml in an indirect (two
As heparan sulfate proteoglycans (HSPGs) are known as co-receptors to interact with numerous growth factors and then modulate downstream biological activities, overexpression of HS/HSPG on cell surface acts as an increasingly reliable prognostic factor in tumor progression. Cell penetrating peptides (CPPs) are short-chain peptides developed as functionalized vectors for delivery approaches of impermeable agents. On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to multiple cellular effects. Here a functional peptide (CPP|i|ecp|/i|) has been identified from critical HS binding region in hRNase3, a unique RNase family member with|i| in vitro|/i| antitumor activity. In this study we analyze a set of HS-binding CPPs derived from natural proteins including CPP|i|ecp|/i|. In addition to cellular binding and internalization, CPP|i|ecp|/i| demonstrated multiple functions including strong binding activity to tumor cell surface with higher HS
Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol. ...
Definition of Macrophage migration-inhibitory factors with photos and pictures, translations, sample usage, and additional links for more information.
Cellular immunity to the hepatitis B surface antigen (HBsAg) and a liver-specific lipoprotein was studied, using the leucocyte migration test, in 38 asymptomatic blood donors found to have HBsAg in the serum. Sensitization to HBsAg was found in 26% and was related to the presence of liver damage, being detected in 47% of those with elevated serum aspartate aminotransferase but in only 13% with normal enzyme levels. The frequency of sensitization to this antigen in those with chronic persistent or chronic aggressive hepatitis on biopsy was also higher than in those with unrelated or minimal changes ...
Based on the food item, the conditions of use, and contact time and temperature, plastic food packaging materials must be assessed in terms of their migration.. For companies with a wide range of products, attempting to perform migration tests on each one and for each use represents a huge undertaking. The key to optimizing resources is grouping test samples and conditions, as well as testing only the most restrictive requirements. Careful planning of the testing process helps save time and money for everybody and also prevents future problems.. At AIMPLAS, we not only perform migration tests, but also provide manufacturers of materials and items for food contact with personalized advice, which includes: ...
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University of Pennsylvania researchers have discovered links between skin cancer growth and the presence or absence of certain cancer-related molecules.
Mouse monoclonal antibody raised against native CD34. Native purified CD34 from permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia. (MAB4412) - Products - Abnova
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komakino fanzine - Lykanthea - Migration: Lakshmi Ramgopal, aka Lykanthea, this debut release of her is dated 2014, yet, I found it only a few days ago, and I am stunned - where was I in ...
Years of conversation fill a ton of digital pages, and weve kept all of it accessible to browse or copy over. Whether youre looking for reveal articles for older champions, or the first time that Rammus rolled into an OK thread, or anything in between, you can find it here. When youre finished, check out the boards to join in the latest League of Legends discussions. GO TO BOARDS ...
Macrophage Migration-Inhibitory Factors definition. define Macrophage Migration-Inhibitory Factors. Explain Macrophage Migration-Inhibitory Factors. What is Macrophage Migration-Inhibitory Factors? Macrophage Migration-Inhibitory Factors FAQ.
TY - JOUR. T1 - Autoimmune thyroiditis, adrenalitis and oophoritis. AU - Edmonds, Merrill. AU - Lamki, Lamk. AU - Killinger, Donald W.. AU - Volpé, Robert. PY - 1973. Y1 - 1973. N2 - Described here is a young woman suffering from autoimmune thyroiditis, adrenalitis and oophoritis. This patient was carefully investigated by endocrine studies, with humoral antibodies to thyroid and adrenal, and the release of migration inhibition factor by her lymphocytes when cultured with thyroid, adrenal and ovarian antigens. Cell-mediated immunity appears to be the most important factor in the pathogenesis of these closely related disorders. A discussion of the interrelationship of these organ-specific autoimmune endocrine gland disorders is presented.. AB - Described here is a young woman suffering from autoimmune thyroiditis, adrenalitis and oophoritis. This patient was carefully investigated by endocrine studies, with humoral antibodies to thyroid and adrenal, and the release of migration inhibition factor ...
TY - JOUR. T1 - Terbinafine inhibits endothelial cell migration through suppression of the Rho-mediated pathway. AU - Ho, Pei Yin. AU - Zhong, Wen-Bin. AU - Ho, Yan Soon. AU - Lee, Wen Sen. PY - 2006/12. Y1 - 2006/12. N2 - We showed previously that terbinafine, an allylamine with fungicidal activity, could inhibit angiogenesis by suppressing the endothelial cell proliferation. In the present study, we further showed that terbinafine (0-120 μmol/L) dose dependently inhibited the adhesion and migration of human umbilical vascular endothelial cells (HUVEC). Western blot analysis showed that terbinafine decreased the levels of Ras protein and membrane-bound RhoA protein. Moreover, the terbinafine-induced migration inhibition in HUVEC was prevented by pretreatment with farnesol or geranylgeraniol. Pretreatment of HUVEC with Ras inhibitor peptide or a ROCK (a kinase associated with RhoA for transducing RhoA signaling) inhibitor, Y27632, abolished the farnesol- or geranylgeraniol-induced prevention ...
FIGURE 3. IFN-β reduces cell migration through IFIT2 expression. A. Enhanced expression of IFIT2 following IFN-β treatment. Logarithmically growing OC3 cells were treated with various concentrations of IFN-β for 24 h. RT-PCR (left) and Western blot (right) analysis of IFIT2 in OC3 cells treated with various doses of IFN-β (0-500 units/mL). At the end of treatment, the amount of IFIT2 induced by IFN-β was analyzed by RT-PCR (GAPDH was used as the loading control) and Western blotting (β-actin was used as the loading control). All experiments were done thrice, and one representative experiment is shown. B. Antiproliferation effect of IFN-β on OC3 cells. Cells were treated with various concentrations of IFN-β for 24 or 48 h. Cells were counted and examined for viability by trypan blue dye exclusion. Points, average of three independent experiments; bars, SD. C. Migration inhibition of IFN-β on OC3 cells. Cells were seeded in the upper chamber of a Transwell cell culture system, and after ...
|p|One of the first cytokines described, MIF (macrophage migration inhibitory factor) was originally identified in studies of delayed hypersensitivity reactions where it was shown to inhibit macrophage migration. It is an important mediator of the innate immune response with potential roles in the pathophysiology of inflammatory, autoimmune, and neoplastic disorders. The human MIF gene encodes a 115 amino acid, 12.5 kDa secreted protein. Crystallographic studies suggest that MIF exists as a homotrimer, although some reports show that it may exist as a dimer or monomer as well. Although MIF exhibits no homology with other known cytokines, it shares structural homology with several bacterial enzymes. It has been speculated that MIF is an inflammatory mediator possibly associated with rheumatoid arthritis (RA) severity.|/p|
HLDA WorkshopHLDA VI-WS Code M MA58Quantity100 testsVolume0.4ImmunogenPermanent human cell line derived from peripheral leucocytes of a patient suf...
By Cindy Bontrager. The 2017 space migration project is underway to reallocate spaces vacated as a result of the 2016 space migration project. The space migration process allows the university to make strategic, transparent space changes that provide long-term benefits to campus. This phase includes space in Anderson, Fairchild, Holton, Leasure and Seaton halls, as well as Hale Library and Unger Complex - the former old Foundation building. Additional information regarding the available space as well as a schedule of building tours and proposal guidelines is available on the space migration website.. The process for submitting and reviewing space migration proposals will follow a similar format as the previous projects. Concept proposals must be approved and submitted by a dean or vice president by Dec. 16. Final space migration outcomes are expected to be announced by April 17, 2017. The general project timeline and working group members can be found on the space migration website. Other ...
The Mammalian Phenotype (MP) Ontology is a community effort to provide standard terms for annotating phenotypic data. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated phenotype data at MGI are provided in Term Detail reports.
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Methods The migratory ability of peripheral neutrophils from healthy donors (HD) and different tumour cell lines (myeloid leukaemia HL60 cells and human pancreatic adenocarcinoma COLO357 cells) was analysed in response to N-Formylmethionyl-leucyl-phenylalanine (FMLP) or Protease-activated receptor 2 (Par-2) agonist. Because it has been shown before [2], IgGs from HD and SSc patients were used as additional stimulus for migration. Neutrophils and HL60 cells were preincubated (1h) with sitaxentan or ambrisentan, respectively, before being tested for migration (1h) using the Transwell assay. COLO357 cells were incubated (48h) in the presence of sitaxentan and migration was tested in the Oris Pro Cell assay. Migration was analysed by automatic cell counting or digital photo analysis and a migration index was calculated. ...
Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, is considered an attractive therapeutic target in multiple inflammatory and autoimmune disorders. In addition to its known biologic activities, MIF can also function as a tautomerase. Several small molecules have been reported to be effective inhibitors of MIF tautomerase activity in vitro. Herein we employed a robust activity-based assay to identify different classes of novel inhibitors of the catalytic and biological activities of MIF. Several novel chemical classes of inhibitors of the catalytic activity of MIF with IC(50) values in the range of 0.2-15.5 microm were identified and validated. The interaction site and mechanism of action of these inhibitors were defined using structure-activity studies and a battery of biochemical and biophysical methods. MIF inhibitors emerging from these studies could be divided into three categories based on their mechanism of action: 1) molecules that covalently modify the catalytic site at
Peptides , Prion Protein (PrP) Fragments , PrP (106-126); This peptide increases membrane microviscosity, intracellular Ca2+ concentration and cell migration in circulating leucocytes, and oxygen production in monocytes and neutrophils. It also induces apoptotic cell death and impairment of L-type voltage-sensitive calcium channel activity in the GH3 cell lines. PrP (106 126) stimulates leucocyte migration in a dose-dependent manner.; KTNMKHMAGAAAAGAVVGGLG; H-Lys-Thr-Asn-Met-Lys-His-Met-Ala-Gly-Ala-Ala-Ala-Ala-Gly-Ala-Val-Val-Gly-Gly-Leu-Gly-OH
Macrophage migration inhibitory factor (MIF) is an evolutionary conserved 12.5-kDa protein mediator with multiple functions in innate and acquired immunity. Upon leaderless secretion, MIF acts as a typical inflammatory cytokine, but there is no structural homology between MIF and any of the known cy …
We are investigating the role of DNA repair in prevention of cancer and in human development. We perform clinical, molecular, and translational investigations of two rare genetic disorders with defective DNA repair: xeroderma pigmentosum (XP) with clinical and cellular hypersensitivity to ultraviolet radiation and a 10,000-fold increased risk of skin cancer and
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Migration is a natural human phenomenon, but not one that organisations in the built environment are that focused on. City planners can anticipate the radical societal change generated by migration, in order to help cities, their populations and industries to prepare.
sundoc Migration; Titel: Funktionelle Charakterisierung von Teilschritten des Tat-abhängigen Proteintransports an der Thylakoidmembran, Verfasser: Frielingsdorf, Stefan, 2008 ; Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt
sundoc Migration; Titel: Kardiomyopathien im Kindesalter, Verfasser: Friedrich, Christiane, 2002 ; Halle, Saale : Universitäts- und Landesbibliothek
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PubMedID: 23536817 | Macrophage migration inhibitory factor inhibition is deleterious for high-fat diet-induced cardiac dysfunction. | PloS one | 1/1/2013
Bay-Richter et al. Journal of Neuroinflammation (2015) 12:163 DOI /s JOURNAL OF NEUROINFLAMMATION RESEARCH Behavioural and neurobiological consequences of macrophage migration inhibitory
Blocking of monocyte migration induced by supernatant of RA SCL stimulated with TNF-α. Monocyte migration induced by supernatants of RA SCLs (RA6/1 and RA8/3)
Y. Mizue, S. Ghani, L. Leng, C. McDonald, P. Kong, J. Baugh, S. J. Lane, J. Craft, J. Nishihira, S. C. Donnelly, Z. Zhu, R. Bucala ...
1MFI: Crystal structure of macrophage migration inhibitory factor complexed with (E)-2-fluoro-p-hydroxycinnamate at 1.8 A resolution: implications for enzymatic catalysis and inhibition.
1MFI: Crystal structure of macrophage migration inhibitory factor complexed with (E)-2-fluoro-p-hydroxycinnamate at 1.8 A resolution: implications for enzymatic catalysis and inhibition.
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Cell-mediated immunity is one of the two types of adoptive immune system in the human body. Its generally used to fight microbes...
Subject: RE: DATAFILE?? The best way to find out is to try it. You will probably find that it depends. If you use a LMT and do uniform extents then it will do it by concatenation. (fill up the first file then move on to the 2nd etc.). If you create an LMT and do automatic extents then it is different. It does it by striping. The first extent goes in the first file, the 2nd in the 2nd file... You can see this by setting up a small test. Jim -----Original Message ...
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Vekom klesá hladina koenzýmu Q10 pod optimálnu hladinu, kolagén a elastín sa nevytvára v dostatočnom množstve, pokožka je vystavená k vyššiemu poškodeniu voľnými radikálmi. Koenzým Q10 podporuje tvorbu kolagénu a elastínu, napomáha spustiť proces obnovy pokožky, jej regeneráciu a redukuje poškodenia spôsobené voľnými radikálmi. Prenáša kyslík a je malou molekulou, ktorá sa relatívne rýchlo dostane do kožných buniek ...
May deprive the cell of certain nutrients, altering the proteins that the cell produces and interfering with normal cell functioning and cell ...
"Inhibition of SNARE-mediated membrane traffic impairs cell migration". Experimental Cell Research. 305 (1): 63-73. doi:10.1016/ ... Bves demonstrates dynamic localization, dependent upon cell-cell junction formation. Prior to cell-cell contact, Bves is ... Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho ... "Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion". ...
Further, this process promotes epithelial cell migration, proliferation and apoptosis. For menstruation, it promotes the ... and only hematopoietic cells remained after that time. In order for MMPs to escape TIMP inhibition, active MMP7s are recruited ... In human glioma cell lines and human squamous cell carcinoma cell line II-4, TGF-β stimulates the expression of MMP7 mRNA and ... MMP7 cleaves cell surface proteins, promotes adhesion of cancer cells, and increases the potential of tumor metastasis. Higashi ...
"KIF1A inhibition immortalizes brain stem cells but blocks BDNF-mediated neuronal migration". Nature Neuroscience. 19 (2): 253- ... cell body to axon terminal) and dendritic (cell body to dendrites) transport of cargo. The main function of KIF1A is the long- ... Molecular Cell Biology. 10 (12): 877-84. doi:10.1038/nrm2807. PMID 19935670. Al-Bassam J, Nithianantham S (December 2018). " ... KIF1A is one of the many motors that helps execute the transport of organelles within the cell through axonal anterograde cargo ...
... monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration". Cardiovascular ... In skin, Langerhans cells strongly express ALOX5. Fibroblasts, smooth muscle cells and endothelial cells express low levels of ... mast cells, dendritic cells, and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. ... and the pharmacological inhibition of ALOX5 in these human cell lines causes them to die by entering apoptosis.[18][19][20][21] ...
Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by ...
inhibit cell migration of endothelial cells. ramucirumab. inhibition of VEGFR2[33]. ... inhibit cell proliferation of endothelial cells. thrombospondin. inhibit cell migration, cell proliferation, cell adhesion and ... inhibit cell proliferation and induce apoptosis of endothelial cells. endostatin. inhibit cell migration, cell proliferation ... inhibit cell proliferation and induce apoptosis of endothelial cells. endostatin. inhibit cell migration, cell proliferation ...
"Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates: possible links between cell ... and migration. Regulation[edit]. Allosteric regulation by metabolites. The regulation of the citric acid cycle is largely ... In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria, ... Pink nodes: cell signaling. Blue nodes: amino acid metabolism. Grey nodes: vitamin and cofactor metabolism. Brown nodes: ...
GABA regulates the proliferation of neural progenitor cells[12][13] the migration[14] and differentiation[15][16] the ... Shunting inhibition has no direct effect on the membrane potential of the cell; however, it reduces the effect of any ... GABA can promote the replication and survival of β-cells[23][24][25] and also promote the conversion of α-cells to β-cells, ... "Long-Term GABA Administration Induces Alpha Cell-Mediated Beta-like Cell Neogenesis". Cell. 168 (1-2): 73-85.e11. doi:10.1016/j ...
... angiogenesis and cell migration. Homologous subfamily of ADAMTSL (ADAMTS-like) proteins, which lack enzymatic activity, has ... Most cases of thrombotic thrombocytopenic purpura arise from autoantibody-mediated inhibition of ADAMTS13. Like ADAMs, the name ... Cell Biology. 15 (6): 981-985. doi:10.1016/j.biocel.2004.01.014. PMID 20036837. Kelwick, Richard; Desanlis, Ines; Wheeler, ...
This interaction inhibits the activation of MEK and ERK and even cell migration. In cancer tissues, where ARHI is not expressed ... ARHI influences also the cell cycle, specifically ARHI's strong inhibition of the cyclin D1 promoter. Cyclin D1 is an essential ... "The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration". Biology of the ... and its regulation by ARHI is critical in maintaining healthy cells. This is the mechanism by which ARHI inhibits cell growth ...
Yang H, Ganguly A, Cabral F (October 2010). "Inhibition of cell migration and cell division correlates with distinct effects of ... Severe side effects include low blood cell counts and shortness of breath. It should not be given to people who have a current ... Vinblastine works by blocking cell division. Vinblastine was isolated in 1958. An example of a natural herbal remedy that has ... Vinblastine treatment causes M phase specific cell cycle arrest by disrupting microtubule assembly and proper formation of the ...
... and invasion of malignant cells. Other studies suggest that crizotinib might also act via inhibition of angiogenesis in ... Crizotinib is currently thought to exert its effects through modulation of the growth, migration, ... In March 2016, the U.S. Food and Drug Administration approved crizotinib in ROS1-positive non-small cell lung cancer. In ... About 4% of patients with non-small cell lung carcinoma have a chromosomal rearrangement that generates a fusion gene between ...
PLCγ1 is a cell growth factor from the PLC superfamily. PLCγ1 is used during the cell growth and in a cell migration and ... Mutations in PLCγ1 can lead to cancer cell proliferations and inhibition can lead to tumor growth. PLCγ1 is involved in cell ... This aspect of PLCγ1 also helps cancer migration and metastasis away from the original tumor cells. There is also a link ... Phospholipase C, gamma 1, also known as PLCG1,is a protein that in humans involved in cell growth, migration, apoptosis, and ...
Yang H, Ganguly A, Cabral F (October 2010). "Inhibition of cell migration and cell division correlates with distinct effects of ... Lower concentration affects microtubule dynamics and cell migration. Demecolcine is used for scientific research in cells. It ... thus arresting cells in metaphase and allowing cell harvest and karyotyping to be performed. During cell division, demecolcine ... Demecolcine inhibition of microtubules causes aneuploidy in mitotic cells where the microtubules fall apart or are suppressed ...
On the other hand, silencing RhoA lessened androgen-regulated cell viability and handicapped prostate cancer cell migration. ... Inhibition of this pathway by its various components usually results in some level of improved re-myelination. After global ... rhoA+Protein at the US National Library of Medicine Medical Subject Headings (MeSH) RHOA Info with links in the Cell Migration ... A majority of this activity occurs in the leading edge of cells during migration in coordination with membrane protrusions of ...
Franco SJ, Huttenlocher A (September 2005). "Regulating cell migration: calpains make the cut". Journal of Cell Science. 118 ( ... Grieve S, Gao Y, Hall C, Hu J, Greer PA (August 2016). "Calpain Genetic Disruption and HSP90 Inhibition Combine To Attenuate ... There is evidence suggesting that the mechanism of action is through cleavage of substrates involved in cell migration, ... Hanna RA, Campbell RL, Davies PL (November 2008). "Calcium-bound structure of calpain and its mechanism of inhibition by ...
... "miR-340 inhibition of breast cancer cell migration and invasion through targeting of oncoprotein c-Met". Cancer. 117 (13): 2842 ... "Micro-RNA dysregulation in multiple sclerosis favours pro-inflammatory T-cell-mediated autoimmunity". Brain. 134 (Pt 12): 3578- ...
... caused the inhibition of recombinant interleukin-8 (IL-8) induced migration of the inflammatory cells. Two other pro- ... Gastric parietal cells are rich in mitochondria which provide energy in the form of ATP for cells by oxidative phosphorylation ... They also inhibit mast cell activation, and leukocyte and platelet adherence to the vascular endothelium. Thus, continuous ... In addition to inhibition of pro-inflammatory mediators, troxipide directly acts on the enzymes such as xanthine oxidase and ...
"Sesquiterpene lactone suppresses vascular smooth muscle cell proliferation and migration via inhibition of cell cycle ... 265-. ISBN 0-8223-1019-8. Amaya S, Pereira JA, Borkosky SA, Valdez JC, Bardón A, Arena ME (October 2012). "Inhibition of quorum ...
Wnt signaling functions can be divided into axis patterning, cell fate specification, cell proliferation and cell migration. In ... PDE mediates this through the inhibition of PKG, which subsequently causes the inhibition of calcium release. The binary ... Cell fate specification or cell differentiation is a process where undifferentiated cells can become a more specialized cell ... Wnt signaling also induces cell migration in later stages of development through the control of the migration behavior of ...
Molecular Mechanisms by Which S100A4 Regulates the Migration and Invasion of PGCCs With Their Daughter Cells in Human ... It is concluded that extracellular S100A4 inhibition is an attractive approach for the treatment of human cancer. S100A4 is ... This antibody abolished endothelial cell migration, tumor growth and angiogenesis in immunodeficient mouse xenograft models of ... in promoting endothelial cell migration by increasing KDR expression and MMP-9 activity. In vivo overexpression of S100A4 led ...
Also, the migration of adult neural progenitor cell and adult spinal cord progenitor cells to the spine is netrin 1 dependent. ... Little is known of the mechanism controlling the inhibition or attraction of these stem cells. In various human cancers, it has ... Netrin contributes to tissue morphogenesis by controlling developing cell migration and cell adhesion in different organs. In ... Von Hilchen, C. M.; Hein, I.; Technau, G. M.; Altenhein, B. (2010). "Netrins guide migration of distinct glial cells in the ...
S100A4 expression is associated with enhanced cell migration through maintenance of cell polarization and inhibition of cell ... Ravid S (2014). "The tumor suppressor Lgl1 regulates front-rear polarity of migrating cells". Cell Adhesion & Migration. 8 (4 ... cell migration, polarization and adhesion, maintenance of cell shape, and signal transduction. Myosin IIs are motor proteins ... "Non-muscle myosin II takes centre stage in cell adhesion and migration". Nature Reviews. Molecular Cell Biology. 10 (11): 778- ...
It also induces apoptosis in leukocyte cancer cells and affects the invasion, migration and metastasis of those cells. This is ... with outliers of 30.22 and 116.96 μg/ml for the growth inhibition of AGS and Hepa1c1c7 cells respectively. The cytotoxic effect ... It has been shown to inhibit cell proliferation in certain cancer cell lines (AGS and Hepa 1c1c7 cells), which is possible by, ... Cynaropicrin inhibits the entry of pan-genomic Hepatitis C virus into cells and inhibits cell-cell transmission. Cynaropicrin ...
... acid exhibits potent antitumor effects against colorectal cancer via inhibition of cell proliferation and migration". ...
"Inhibition of Cell Migration and Cell Division Correlate with Distinct Effects of Microtubule Inhibiting Drugs". Journal of ... Journal of Cell Science. 20 (1): 79-89. PMID 942954. Archived (PDF) from the original on 2014-01-13.. ... Adverse effects of vinblastine include hair loss, loss of white blood cells and blood platelets, gastrointestinal problems, ... non-small cell lung cancer, bladder cancer, brain cancer, melanoma, and testicular cancer.[1] It is given by injection into a ...
In epithelial cells inhibition of ROCK seems to decrease tight junction integrity. Active ROCK in these cells seems to ... For example, such drugs have potential to prevent cancer from spreading by blocking cell migration, stopping cancer cells from ... ROCKs regulate cell-cell adhesion: Loss of ROCK activity seems to lead to loss of tight junction integrity in endothelial cells ... Cellular contractility ROCK also regulates cell migration by promoting cellular contraction and thus cell-substratum contacts. ...
"Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein- ... Kinases of GCKIII subfamily are involved in regulation of multiple functions of the cells and interaction with programmed cell ... "CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation". Journal of Cell Science. 123 (Pt 8): ... In the cells, STK24 is located in nucleus and less in cytoplasm and membrane. There are two isoforms of the protein, isoform A ...
This can lead to the formation of stress fibers and cell migration through the inhibition of myosin light-chain phosphatase. ... "Lysophosphatidate-induced cell proliferation: Identification and dissection of signaling pathways mediated by G proteins". Cell ... 2010). "Lysophosphatidic acid augments human hepatocellular carcinoma cell invasion through LPA1 receptor and MMP-9 expression ... Because of its ability to stimulate cell proliferation, aberrant LPA-signaling has been linked to cancer in numerous ways. ...
Any changes in cytoskeletal organization and adhesion can lead to altered signaling, migration and a loss of contact inhibition ... F9 embryonal carcinoma cells are similar to the P19 cells shown in Figure 1 and normally have cell-to-cell adhesion mediated by ... A tumor cell line with defective δ-catenin, low levels of E-cadherin and poor cell-to-cell adhesion could be restored to normal ... providing the cell with a means of stable cell adhesion. However, decreases in this adhesion ability of the cell has been ...
Cell growth, proliferation, angiogenesis, and migrationEdit. The above processes are part and parcel to tissue integrity and ... Currently, the only completely developed method available in that regard is antagonism (blockade, inhibition) of the SP ... Substance P has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ...
epithelial cell proliferation. • neuron migration. • negative regulation of apoptotic process. • negative regulation of ... suggesting that deficiency of presenilin-1 can down regulate amyloid and inhibition of presenilin-1 can be a potential method ... cell nucleus. • kinetochore. • centrosome. • rough endoplasmic reticulum. • dendritic shaft. • aggresome. • cell surface. • ... cell cortex. • integral component of membrane. • azurophil granule membrane. • Z disc. • neuronal cell body. • perinuclear ...
There, CRH and vasopressin act synergistically to stimulate the secretion of stored ACTH from corticotrope cells. ACTH is ... and inhibition of aggression. Inclusion of the amino acid L-tryptophan, a precursor of 5HT, in the feed of rainbow trout made ... by encouraging migration (i.e. fleeing), the mobilization of energy, learning (in the face of novel, dangerous stimuli) as well ... in immune cells, such as monocytes and neutrophils [8][9][11][12] ...
... discovery of Rho GTPases that play a role in cytoskeleton arrangement and cell migration and its application to cancerous cells ... The inhibition of Rho by C3 transferase ribosylation resulted in an inhibition of focal adhesion and stress fibre assembly, but ... DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ... migration and polarity of the cell cytoskeleton was awarded the Louis Jeantet Prize for Medicine.[14] Later that year he won ...
cell-cell signaling. • positive regulation of brain-derived neurotrophic factor receptor signaling pathway. • collateral ... This becomes especially evident following suppression of TrkB activity.[30] TrkB inhibition results in a 2-3 fold increase in ... By stabilizing p35 (CDK5R1), in utero electroporation studies revealed BDNF was able to promote cortical radial migration by ~ ... regulation of protein localization to cell surface. • regulation of receptor activity. • activation of phospholipase C activity ...
Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... For example, a small-molecule mimetic of Smac/Diablo that counteracts the inhibition of apoptosis has been shown to enhance ... Evidence shows that autocrine VEGF is involved in two major aspects of invasive carcinoma: survival and migration. Moreover, it ... These T cells can then go on to perform effector functions such as macrophage activation, B cell activation, and cell-mediated ...
May 2012). "Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell ... Several recent studies have reported that preventing OEC inhibition will present a uniform population of cells in the spinal ... be rejected by the body and biological functions such as cell adhesion and growth will be enhanced through cell-cell and cell- ... "CD46 on glial cells can function as a receptor for viral glycoprotein-mediated cell-cell fusion". Glia. 52 (3): 252-8. doi: ...
Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition,Cell, Volume 146, ... Cell migration. *Endocytic cycle. *Epsin. *Synaptic vesicle. References[edit]. *^ Pearse BM (1976). "Clathrin: a unique protein ... "Journal of Cell Biology. 202 (3): 463-78. doi:10.1083/jcb.201211127. PMC 3734082 . PMID 23918938.. ... "J Cell Biol. 202 (3): 463-78. doi:10.1083/jcb.201211127. PMC 3734082 . PMID 23918938.. ...
Wang G, Sun J, Liu G, Fu Y, Zhang X (December 2017). "Bradykinin Promotes Cell Proliferation, Migration, Invasion, and Tumor ... It is thought that bradykinin is converted to inactive metabolites by ACE, therefore inhibition of this enzyme leads to ... Bradykinins have been implicated in cell proliferation and migration in gastric cancers,[18] and bradykinin antagonists have ... Moreover, endothelial cells have been described as a potential source for this B1 receptor-CXCL5 pathway.[11] ...
leukocyte migration. • lipopolysaccharide-mediated signaling pathway. • positive regulation of smooth muscle cell proliferation ... showed that TNFα causes an IL-10-dependent inhibition of CD4 T-cell expansion and function by up-regulating PD-1 levels on ... positive regulation of heterotypic cell-cell adhesion. • negative regulation of mitotic cell cycle. • endothelial cell ... but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac ...
In vitro proliferation of MM cell lines and inhibition of Fas-mediated apoptosis is promoted by IL-6. Thalidomide and its ... bFGF and IL-6 appear to be required for endothelial cell migration during angiogenesis. Thalidomide and its analogs are ... Inhibition of TNF-α is not the mechanism of thalidomide's inhibition of angiogenesis since numerous other TNF-α inhibitors do ... They have also been shown to cause dose dependent G0/G1 cell cycle arrest in leukemia cell lines where the analogs showed 100 ...
"Stroke and T-cells". Laboratory of Neurosciences, National Institute on Aging Intramural Research Program; Arumugam TV, Granger ... "Poststroke neurogenesis: emerging principles of migration and localization of immature neurons". David Geffen School of ... "Reducing excessive GABA-mediated tonic inhibition promotes functional recovery after stroke". Department of Neurology, The ... sickle cell anemia. Trombositemia dan sejenisnya. *Hypercoaguable states-puerperium. oral contraceptive use. 'sticky platelet ...
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. • positive regulation of leukocyte migration. • ... indicating the inhibition of interaction between tumor cell and endothelial cell is significant for blocking tumor ... leukocyte migration. • response to lipopolysaccharide. • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion ... Ryan US, Worthington RE (February 1992). "Cell-cell contact mechanisms". Curr. Opin. Immunol. 4 (1): 33-7. doi:10.1016/0952- ...
Common side effects may include low platelets, low white blood cells, anemia, rashes, vomiting, diarrhea, and joint pains.[3] ... "Induction and inhibition of cytochromes P450 by the St. John's wort constituent hyperforin in human hepatocyte cultures". Drug ... Migration-stimulating factor (MSF; PRG4). *Oncomodulin. *Pituitary adenylate cyclase-activating peptide (PACAP) ... and reduced blood cell count. Less typical side effects are those of the cardiovascular system, such as high blood pressure, ...
positive regulation of cell migration. • blood coagulation. • proteolysis. • ER to Golgi vesicle-mediated transport. • blood ... Inhibition of the synthesis or activity of Factor X is the mechanism of action for many anticoagulants in use today. Warfarin, ... In stage 1, Factor VII binds to the transmembrane protein TF on the surface of cells and is converted to Factor VIIa. The ... Factor Xa formed on the surface of the TF-bearing cell interacts with Factor Va to form the prothrombinase complex which ...
"Protein & Cell. 6: 363-72. doi:10.1007/s13238-015-0153-5. PMC 4417674 . PMID 25894090. Retrieved 24 April 2015.. ... Goldstein DB, Chikhi L (2002). "Human migrations and population structure: what we know and why it matters". Annu Rev Genom Hum ... inhibition of other enzymes in the biochemical pathway to prevent buildup of a toxic compound, or diversion of a toxic compound ... Each cell of the body contains the hereditary information (DNA) wrapped up in structures called chromosomes. Since genetic ...
... monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration". Cardiovascular ... In skin, Langerhans cells strongly express ALOX5. Fibroblasts, smooth muscle cells and endothelial cells express low levels of ... mast cells, dendritic cells, and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. ... and the pharmacological inhibition of ALOX5 in these human cell lines causes them to die by entering apoptosis.[18][19][20][21] ...
This can occur when cells become overcrowded (density-dependent inhibition) or when they differentiate to carry out specific ... "Interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia". Current Biology ... Related cell processes[edit]. Cell rounding[edit]. Cell shape changes through mitosis for a typical animal cell cultured on a ... In animal cells, a cell membrane pinches inward between the two developing nuclei to produce two new cells. In plant cells, a ...
CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. ... Fernandez EJ, Lolis E (2002). "Structure, function, and inhibition of chemokines". Annual Review of Pharmacology and Toxicology ... CCR9 supports the migration of leukocytes into the intestine, CCR10 to the skin and CXCR5 supports the migration of B-cell to ... The major role of chemokines is to act as a chemoattractant to guide the migration of cells. Cells that are attracted by ...
... that halts the growth of cancer cells in mice and also stops the migration of the tumors to other sites.[24] However, this is ... in the inhibition of tumor progression and prolongation of survival in a rodent glioma model". J. Neurosurg. 103 (3): 526-537. ... "Snake Venom Protein Paralyzes Cancer Cells". Journal of the National Cancer Institute. 93 (4): 261-262. doi:10.1093/jnci/93.4. ...
cell surface receptor signaling pathway. • receptor internalization. • dendritic cell chemotaxis. • signal transduction. • ... Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing ... "Inhibition of interleukin-8 (CXCL8/IL-8) responses by repertaxin, a new inhibitor of the chemokine receptors CXCR1 and CXCR2". ... Cell Genet. 63 (4): 238-40. doi:10.1159/000133541. PMID 8500355.. *. Damaj BB, McColl SR, Mahana W, Crouch MF, Naccache PH ( ...
The latter contributes to the control of cell migration. In this way, src-kinase deregulation can enhance tumor growth and ... "Small-molecule inhibition and activation-loop trans-phosphorylation of the IGF1 receptor". The EMBO Journal. 27 (14): 1985-94 ... "Cell. 138 (3): 514-24. doi:10.1016/j.cell.2009.05.028. PMC 4764080. PMID 19665973.. ... "Molecular Cell. 55 (6): 891-903. doi:10.1016/j.molcel.2014.08.006. PMC 4169746. PMID 25201411.. ...
The osteoclasts are multi-nucleated cells that contain numerous mitochondria and lysosomes. These are the cells responsible for ... "Protective effects of estradiol on ethanol-induced bone loss involve inhibition of reactive oxygen species generation in ... "Sphingosine-1-phosphate-mediated osteoclast precursor monocyte migration is a critical point of control in antibone-resorptive ... The osteoclast then induces an infolding of its cell membrane and secretes collagenase and other enzymes important in the ...
endothelial cell migration. • phosphatidylinositol-mediated signaling. • leukocyte migration. • ERBB2 signaling pathway. • ... Inhibition[edit]. All PI 3-kinases are inhibited by the drugs wortmannin and LY294002 but wortmannin shows better efficiency ... cell migration. • positive regulation of phosphatidylinositol 3-kinase signaling. • phosphatidylinositol biosynthetic process. ... T cell receptor signaling pathway. • activation of protein kinase activity. • regulation of genetic imprinting. • negative ...
homotypic cell-cell adhesion. • negative regulation of apoptotic process. • negative regulation of anoikis. • negative ... Wirth T, Soeth E, Czubayko F, Juhl H (2002). "Inhibition of endogenous carcinoembryonic antigen (CEA) increases the apoptotic ... Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... Cell. Biol. 10 (6): 2738-48. PMC 360634 . PMID 2342461.. *. Kamarck ME, Elting JJ, Hart JT, et al. (1987). "Carcinoembryonic ...
Hu L, Wang Z, Hu C, Liu X, Yao L, Li W, Qi Y (2005). "Inhibition of Measles virus multiplication in cell culture by RNA ... Another potential RNAi-based treatment is to inhibit cell invasion and migration.[156] ... Cullen LM, Arndt GM (June 2005). "Genome-wide screening for gene function using RNAi in mammalian cells". Immunology and Cell ... RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells ...
... angiogenesis and migration of cancer cells by influencing the tumor microenvironment.[66][67] Oncogenes build up an ... DNA repair inhibition is proposed to be a predominant mechanism in heavy metal-induced carcinogenicity. In addition, frequent ... In order for a normal cell to transform into a cancer cell, the genes that regulate cell growth and differentiation must be ... Germ cell tumor: Cancers derived from pluripotent cells, most often presenting in the testicle or the ovary (seminoma and ...
... have noted that tTG transamidation activity could be linked to the inhibition of tumor cell invasiveness. ... differentiation and migration and adhesion .[39][40] Such neurological diseases are characterized in part by the abnormal ... cell membrane. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. • apoptotic cell ... as well as differentiation and cell adhesion.[14] It has been noted that tTG may have very different activity in different cell ...
... significant inhibitory effects on cancer cell migration, and suppression of angiogenesis,i, in vitro,/i, and,i, in vivo,/i,. ... overexpression of HS/HSPG on cell surface acts as an increasingly reliable prognostic factor in tumor progression. Cell ... On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to ... demonstrated multiple functions including strong binding activity to tumor cell surface with higher HS expression, ...
Inhibition of Cancer Cell Migration by Multiwalled Carbon Nanotubes Lorena García-Hevia 1 Rafael Valiente 1 José L. Fernández- ... Inhibition of Cancer Cell Migration by Multiwalled Carbon Nanotubes. Advanced Healthcare Materials, Wiley, 2015, vol. 4 (n° 11 ...
6 JQ1 inhibits the migration and invasion of SACC cells. a The migration of ACC-LM cells and ACC-83 cells treated by JQ1 at ... In this study, we investigated the effect of BRD4 inhibition by JQ1 on cell growth, migration and invasion of SACC cells in ... 7). These results suggest that the inhibition effect of JQ1 on SACC cell migration and invasion may be due to EMT inhibition. ... JQ1 inhibits the migration and invasion of SACC cells. The effect of JQ1 on cell migration and invasion was investigated. The ...
Overexpression of PTEN inhibited cell migration, whereas antisense PTEN enhanced migration. Integrin-mediated cell spreading ... 3 cells/mm2) or sense-transfected cells (0 ± 0 cells/mm2) (Fig. 1A). The number of migrating PTEN-overexpressing cells was ... PTEN modulation of cell migration. Stable NIH 3T3 cell transfectants were induced to express PTEN or antisense PTEN in an ... PTEN inhibition of cell spreading. (A) NIH 3T3 cell transfectants expressing sense or antisense PTEN induced by ecdysone were ...
Triptolide inhibits proliferation and migration of colon cancer cells by inhibition of cell cycle regulators and cytokine ... Triptolide Inhibits Proliferation and Migration of Colon Cancer Cells by Inhibition of Cell Cycle Regulators and Cytokine ... Triptolide Inhibits Proliferation and Migration of Colon Cancer Cells by Inhibition of Cell Cycle Regulators and Cytokine ... Triptolide Inhibits Proliferation and Migration of Colon Cancer Cells by Inhibition of Cell Cycle Regulators and Cytokine ...
In vivo and in vitro inhibition of migration of metastatic melanoma cells. Maxine Emmett, Darryl Dunn, Francoise Cailler, Denis ... In vivo and in vitro inhibition of migration of metastatic melanoma cells ... In vivo and in vitro inhibition of migration of metastatic melanoma cells ... In vivo and in vitro inhibition of migration of metastatic melanoma cells ...
Abstract 722: Inhibition of Endothelial Cell Migration by Semaphorin 3C Requires Neuropilin-1 Uptake via Lipid Rafts. Arie ... Abstract 722: Inhibition of Endothelial Cell Migration by Semaphorin 3C Requires Neuropilin-1 Uptake via Lipid Rafts ... Abstract 722: Inhibition of Endothelial Cell Migration by Semaphorin 3C Requires Neuropilin-1 Uptake via Lipid Rafts ... Abstract 722: Inhibition of Endothelial Cell Migration by Semaphorin 3C Requires Neuropilin-1 Uptake via Lipid Rafts ...
The down-regulation of multiple cytokine receptors, in combination with inhibition of COX-2 and VEGF and positive cell cycle ... Triptolide is a potent inhibitor of colon cancer proliferation and migration in vitro. ... Triptolide inhibits proliferation and migration of colon cancer cells by inhibition of cell cycle regulators and cytokine ... The down-regulation of multiple cytokine receptors, in combination with inhibition of COX-2 and VEGF and positive cell cycle ...
Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and ... Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and ...
... Exp Ther Med. 2017 ... a dose-dependent migration inhibition of A549 in the presence of naringenin was observed by healing and transwell migration ... The current study investigated the mechanisms of naringenin on the migration of lung cancer A549 cells. The results indicate ... Furthermore, naringenin mitigates the migration of several human cancer cell types. However, the effects of naringenin on lung ...
... which is known to play a role in PI 3-kinase-mediated cell migration.31-34⇓⇓⇓ Because PI 3-kinase-mediated cell migration has ... which displayed little cell death. Enhanced apoptosis could also partly account for PTENs inhibition of cell migration. ... Inhibition of Vascular Smooth Muscle Cell Proliferation, Migration, and Survival by the Tumor Suppressor Protein PTEN. Jianhua ... then the cell monolayer was disrupted and cells were treated with or without PDGF (20 ng/mL). A, Migration of cells into the ...
Inhibition of ATX-mediated human MDA-MB-231 cell migration at 10 uM after 8 hrs by Cyto Fluor spectrophotometry relative to ...
SERCA Cysteine-674 Is Required for NO-Mediated Inhibition of HEK Cell Migration. After validating the HEK cell model, migration ... As in experiments in HEK cells, inhibition of VSMC migration by NO after 6 hours reached significance, and the inhibition by ... Because Ca2+ is an important second messenger for cell migration, we hypothesized that NO also inhibits cell migration through ... and NO-induced inhibition of Ca2+influx, which consequently could affect Ca2+ dependent cell functions, such as cell migration ...
... inhibiting cell migration and invasion and suppressing PI3K/AKT/m-TOR signaling pathway. PMID: 31631173 [PubMed - in process ... our results showed that Carnosic acid has the potential to inhibit cancer cell growth in A-549 lung cancer cells by activating ... Antiproliferative Activity of Carnosic Acid is Mediated via Inhibition of Cell Migration and Invasion, and Suppression of ... ïve Participants With Anti-programmed Cell Death Receptor Ligand 1 (PD-L1) Positive Advanced Non-small Cell Lung Cancer (NSCLC ...
Abstract 4026: Attenuation of pancreatic cancer cell migration and invasion through a targeted inhibition of the Rac GEF Vav1. ... Abstract 4026: Attenuation of pancreatic cancer cell migration and invasion through a targeted inhibition of the Rac GEF Vav1 ... Attenuation of pancreatic cancer cell migration and invasion through a targeted inhibition of the Rac GEF Vav1. [abstract]. In ... Abstract 4026: Attenuation of pancreatic cancer cell migration and invasion through a targeted inhibition of the Rac GEF Vav1 ...
Inhibition of cell migration induced by various doses of ferruginol in OVCAR-3 human ovary cancer cells. Cells were treated ... The effects of ferruginol on cell apoptosis, cell migration and cell cycle phase distribution were also evaluated. Cell ... The results indicated that the untreated cells did not exhibit any inhibition of cell migration after 24 h incubation with ... Ferruginol also led to inhibition of cancer cell migration in a dose-dependent manner. The percentage of migrated cells ...
GLPs inhibit cell migration in LoVo cells. At 48 h, the scratch wounds were completely repaired by the migration of untreated ... Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum. ... Cell migration serves an important role in numerous physiological processes, such as cell differentiation and proliferation (39 ... Doyle AD, Petrie RJ, Kutys ML and Yamada KM: Dimensions in cell migration. Curr Opin Cell Biol. 25:642-649. 2013. View Article ...
Inhibition of CXCL8-induced cell migration in human PMN cells at 0.01 uM by chemotaxis assay. ...
... proliferation and migration by oxidative stress from ascorbate-driven juglone redox cycling in human prostate-derived T24 cells ... Inhibition of cell proliferation and migration by oxidative stress from ascorbate-driven juglone redox cycling in human bladder ... Upregulation of prostate-derived Ets factor by luteolin causes inhibition of cell proliferation and cell invasion in prostate ... Inhibition of cell proliferation and migration by oxidative stress from ascorbate-driven juglone redox cycling in human ...
Inhibition of lens epithelial cell migration by acrylic intraocular lens: New evaluation method of optic edge sharpness with ... Inhibition of lens epithelial cell migration by acrylic intraocular lens: New evaluation method of optic edge sharpness with ... H. Ogura, M. Ayaki, H. Nishihara, S. Yaguchi; Inhibition of lens epithelial cell migration by acrylic intraocular lens: New ... was associated with lens epithelial cell (LEC) migration and the formation of posterior capsule opacification (PCO) in the ...
Retraction Note to: FoxM1 down-regulation leads to inhibition of proliferation, migration and invasion of breast cancer cells ...
Inhibition of Protein Kinase Cα Prevents Endothelial Cell Migration and Vascular Tube Formation In Vitro and Myocardial ... Inhibition of Protein Kinase Cα Prevents Endothelial Cell Migration and Vascular Tube Formation In Vitro and Myocardial ... Inhibition of Protein Kinase Cα Prevents Endothelial Cell Migration and Vascular Tube Formation In Vitro and Myocardial ... Inhibition of Protein Kinase Cα Prevents Endothelial Cell Migration and Vascular Tube Formation In Vitro and Myocardial ...
NF Kappa B Inhibitor Prevents the Inflammatory Cell Migration Through the Inhibition of MIP1a Expression in Corneal Penetrating ... NF Kappa B Inhibitor Prevents the Inflammatory Cell Migration Through the Inhibition of MIP1a Expression in Corneal Penetrating ... NF Kappa B Inhibitor Prevents the Inflammatory Cell Migration Through the Inhibition of MIP1a Expression in Corneal Penetrating ... and prevents the migration of inflammatory cells in corneal stroma and anterior chamber. ...
2008). Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay. Cell Res. 18, 479-490. ... 1 implies accelerated cell migration in response to the treatment. Conversely, a ratio ,1 implies inhibition of migration, and ... Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition ... Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition ...
1055865-inhibition-of-glioblastoma-cell-proliferation--migration-and-invasion-by-the-proteasome-antagonist-carfilzomib. ... Inhibition of glioblastoma cell proliferation, migration and invasion by the proteasome antagonist carfilzomib. ...
By using adenoviral-mediated expression of inducible heme oxygenase 1 in primary vascular smooth muscle cells (vsmc) in vivo, ... Targeting of cell cycle regulatory proteins promotes inhibition of cell proliferation, and cell differentiation. Cell cycle ... Helper cell lines may be derived from human cells such as human embryonic kidney cells, muscle cells, hematopoietic cells or ... cell proliferation and migration, and platelet and immune cell adhesion resulting in inhibition of vascular lesion formation ...
About the Journal. As one of the first Open Access journals in its field, Food & Nutrition Research (FNR) offers an important forum for researchers to exchange the latest results from research on human nutrition broadly and food-related nutrition in particular. Learn more about the journals Aims & Scope. FNR is widely indexed by relevant services and databases, including PubMed Central/PubMed, Scopus, Science Citation Index, with an Impact Factor of 2.086 (2017).. ...
Human bladder cancer cells (HT-1376) secrete detectable amounts of MIF protein. Treatment with HA, anti-MIF antibody and MIF ... This study is the first to report MIF expression in the human bladder and these findings support a role for MIF in tumor cell ... The effects of MIF inhibitors (high molecular weight hyaluronate (HA), anti-MIF antibody or MIF anti-sense) on cell growth and ... Our evidence suggests MIF interacts with the invariant chain, CD74 and the major cell surface receptor for HA, CD44. ...
Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility. Oncotarget. ... Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility. In: ... Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility. / Aifuwa, ... title = "Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility", ...
... but had no effect on PDGF-AB-stimulated DNA synthesis or cell migration. Cell migration after submaximal stimulation with ... Polyamine Synthesis Inhibition Attenuates Vascular Smooth Muscle Cell Migration.. Forskningsoutput: Tidskriftsbidrag › Artikel ... Here we investigate the effects of polyamine synthesis inhibition on vascular smooth muscle cell migration after maximal and ... In summary, these data show that polyamine synthesis inhibition attenuates vascular smooth muscle cell migration under ...
  • In addition to cellular binding and internalization, CPP ecp demonstrated multiple functions including strong binding activity to tumor cell surface with higher HS expression, significant inhibitory effects on cancer cell migration, and suppression of angiogenesis in vitro and in vivo . (
  • Alterations of cell surface receptor, coreceptor, and adhesive protein expression are reported in various cancer types in vitro and in vivo [ 1 - 3 ]. (
  • In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). (
  • A twofold overexpression of PTEN mRNA significantly reduced cell migration as measured by in vitro wound healing assays (Fig. 1 ). (
  • A ) In vitro "scratch" wounds were created by scraping confluent cell monolayers in fibronectin-coated (10 μg/ml) petri dishes (35-mm diameter) with a sterile pipette tip. (
  • Triptolide is a potent inhibitor of colon cancer proliferation and migration in vitro. (
  • Flow cytometry was employed to determine the effects of ferruginol on the cell cycle and an in vitro wound healing assay was performed to investigate effects on cancer cell migration. (
  • We used an in vitro model for glioma cell invasion (transwell migration assay) and patch-clamp techniques to investigate the role of volume-activated Cl − currents ( I Cl,Vol ) in glioma cell invasion. (
  • In vitro, the invasion and migration of U2-OS cells were suppressed by inhibiting FASN. (
  • The importance of migration in the development of malignant gliomas explains the interest in studies of this process, in vitro and in vivo ( Chicoine & Silbergeld 1995 ). (
  • showed in vitro that a decrease in GJ communication results in an increase in glioma cells' motility. (
  • is a valuable tool for studying multi-cell migration in vitro. (
  • We previously shown that N-cadherin is vital for VSMC success.11 We now have investigated whether VSMC migration was suffering from perturbation of N-cadherin function using an in vitro migration magic size. (
  • To investigate the effects of microtubule-binding agents (paclitaxel, vinblastine, colchicine, podophyllotoxin), benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelidonine) and other anti-tumor drugs (homoharringtonine, doxorubicin) on cell migration, we performed the in vitro wound healing assay. (
  • In this study, nine cytotoxic natural products (Fig. 1 ) affecting different molecular targets were investigated concerning their effects on cell migration using an in vitro wound healing assay, followed by the study of their interactions with microtubules in GFP co-expressing U2OS cells. (
  • We also show that KU-60019 inhibits glioma cell migration and invasion in vitro , suggesting that glioma growth and motility might be controlled by ATM via AKT. (
  • Rao and his co-authors revealed Slit¿s alter ego ¿ repelling immune cells as well as propelling neurons ¿ via a series of in vitro experiments. (
  • Inhibiting myosin VI expression in high-grade ovarian carcinoma cells impeded cell spreading and migration in vitro . (
  • Inhibiting myosin VI expression substantially impeded migration of ovarian cancer cells in vitro and reduced i.p. dissemination of tumor cells propagated in nude mice. (
  • Automated screening using in vitro or cultured cell assays have yielded thousands of candidate drugs for a variety of biological targets, but these approaches have not resulted in an increase in drug discovery despite major increases in expenditures. (
  • We investigated the effects of selective blockade of a VEGF receptor by using anti-Flt-1 peptide on the formation and hyperplasia of the neointima in balloon injured- carotid arteries of OLETF rats and also on the in vitro VSMCs' migration under high glucose conditions. (
  • And finally, to determine the underlying mechanism of the effect of anti-Flt-1 peptide on the VEGF -induced VSMC migration in vitro , the expression of matrix metalloproteinase (MMP) was observed by performing reverse transcription - polymerase chain reaction (RT-PCR). (
  • In vivo and in vitro granulocyte migration in patients with extrinsic and intrinsic bronchial asthma. (
  • Sixty-five patients with atopic asthma, 54 with infectious asthma, and 30 healthy controls were evaluated by in vitro granulocyte migration and by the in vivo skin window method of Southam. (
  • The migration of tendon cells was evaluated ex vivo by counting the number of initial outgrowths from the tendon explants and in vitro by transwell filter migration assay. (
  • Dose-dependent ibuprofen inhibition was demonstrated on the migration of tendon cells both ex vivo, and in vitro. (
  • In this study, we assessed the expression of Gab1 in 90 CS solid tumors by immunohistochemistry, immunoblotting, and qRT-PCR, and then, some in vitro assays were also applied to CS cells treated with SDF-1. (
  • In the study, we intended to evaluate the effects of multiple targeting of NET-1, EMS1 and VEGF genes on biological behavior of HCC cell in-vitro . (
  • The significance of in vitro hypersensitivity of several cell functions to antigen is discussed in regard to the cell types which have been available in culture. (
  • Cell-cell communication mechanisms are widely studied experimentally (in vivo and in vitro), and computationally (in silico). (
  • Wound healing is commonly studied in vitro using cell lines such as Madin-Darby Canine Kidney cells. (
  • We found that sema3C inhibited the migration of mouse primary endothelial cells (ECs) in Transwell assays by 40%, but it did not inhibit the migration of ECs from synectin −/− mice. (
  • In this report, we investigated the hypothesis that overexpression of the tumor suppressor protein PTEN, an inositol phosphatase specific for the products of PI 3-kinase, would inhibit the vascular smooth muscle cell (VSMC) responses necessary for neointimal hyperplasia and restenosis. (
  • Rapamycin acts specifically to inhibit the activity of mTOR (mammalian target of rapamycin), a serine/threonine kinase that acts as a central controller of cell growth. (
  • CONCLUSIONS In conclusion, our results showed that Carnosic acid has the potential to inhibit cancer cell growth in A-549 lung cancer cells by activating apoptotic death, inhibiting cell migration and invasion and suppressing PI3K/AKT/m-TOR signaling pathway. (
  • Therefore, we hypothesized that azathioprine could also inhibit the Vav1-driven processes of proliferation and invasion in pancreatic tumor cells. (
  • GLPs act as potential immunomodulators that indirectly inhibit cancer cells by increasing the activity of host immune cells ( 14 , 17 ). (
  • Although very low concentrations fail to promote cell movement, optimal concentrations stimulate migration, and excessive levels of attractant inhibit further motility. (
  • 3. The method of claim 2, wherein said biocompatible flexible membrane further comprises patterned surface elements coupled to at least one of said front surface and said back surface of said biocompatible flexible membrane, said patterned surface elements having dimensional relations adapted to inhibit migration of said target cells into said localized region of said eye. (
  • LncRNA MALAT1 expression inhibition can inhibit the proliferation, migration and invasion of tongue cancer cells by inactivating the PI3K/Akt pathway and downregulating MMP-9. (
  • Several studies showed that DHA and EPA together or alone inhibit the growth of breast cancer cell lines [ 17 - 19 ]. (
  • We performed the cerulenin, an inhibitor of FASN, to inhibit FASN expression in U2-OS cells. (
  • The inhibition of FTase by N-benzyl-ACM and N-allyl-ACM seems to be specific, because these two compounds did not inhibit geranylgeranyltransferase or geranylgeranyl pyrophosphate (GGPP) synthase up to 100 μM. (
  • Results from this study show that osthole can not only induce cell death but also inhibit phosphorylation of FAK in human GBM cells. (
  • The benzophenanthridine alkaloid sanguinarine, chelerythrine and chelidonine which affected microtubules in living cells, did not inhibit cell migration. (
  • In the last few years, the targeting of cell migration has become a therapeutically challenging approach for cancer treatment and MBAs have also been reported to inhibit cell migration by interfering with microtubule dynamics [ 22 ]. (
  • However, it is unknown if 17β-estradiol (E 2 ) treatment is sufficient to inhibit cell proliferation and cell migration in human colon cancer cells. (
  • That title,¿ Rao told BioWorld Today , ¿tells the essence of the finding we report ¿ that Slit, a secreted protein previously known for its role of repulsion in axon guidance and neuronal migration, can also inhibit leukocyte migration induced by chemotactic factors. (
  • Now we can inhibit this white-cell migration, and speculate that one might treat these disorders. (
  • This approach demonstrates that the migrating PLLp in zebrafish can be used for large-scale, high-throughput screening for compounds that inhibit collective cell migration and, potentially, anti-metastatic compounds. (
  • Soluble HLA-G was also proven to downregulate CXCR3 manifestation on cytotoxic T cells and inhibit migration along CXCL9 and CXCL10 gradients (56). (
  • We also found that soluble extracellular signals engaging the G protein-coupled receptor Kiss1 (Kiss1R) similarly suppressed EGFR vesicular recycling to inhibit EGF-promoted migration. (
  • In the study, multi-target siRNAs were designed to inhibit NET-1, EMS1 and VEGF genes in hepatocellular carcinoma (HCC) cells. (
  • Importantly, a p27 Kip1 mutant lacking CDK inhibitory activity failed to inhibit vascular smooth muscle cell and fibroblast proliferation and migration. (
  • These findings indicate that the Akt signaling pathway plays a significant role in the migratory activity of Th17 cells from children with LN and suggest that therapeutic modulation of the Akt activity may inhibit Th17 cell trafficking to sites of inflammation and thus suppress chronic inflammatory processes in children with LN. (
  • Triptolide inhibits proliferation and migration of colon cancer cells by inhibition of cell cycle regulators and cytokine receptors. (
  • Overexpression of PTEN significantly inhibited both basal and PDGF-mediated VSMC proliferation and migration, the latter possibly due in part to downregulation of focal adhesion kinase. (
  • miR-24 obviously suppressed the proliferation and migration of MG-63 and U2OS cells. (
  • MiR-623 significantly suppressed XRCC5 expression, which is critical for miR-623-induced proliferation and migration block in breast cancer cells. (
  • In the present study, we treated human LoVo colon cancer cells with E 2 to explore whether E 2 down-regulates cell proliferation and migration, and to identify the precise molecular and cellular mechanisms behind the down-regulatory responses. (
  • The study was aimed to explore the effect of siRNA targeting the YAP gene on cell proliferation and migration of T24 cells . (
  • This study suggested that YAP gene was an important enhancer for the proliferation and migration of bladder cancer cells . (
  • Vascular endothelial growth factor ( VEGF ) plays an important role in angiogenesis, including stimulating the proliferation and migration of vascular smooth muscle cells (VSMCs). (
  • The epidermal growth factor receptor (EGFR) mediates the distinct cellular processes of proliferation and migration, which do not always occur concomitantly upon EGFR stimulation. (
  • Down-regulation of Gab1 inhibits cell proliferation and migration in hilar cholangiocarcinoma. (
  • Previous studies have demonstrated a protective effect of the cyclin-dependent kinase (CDK) inhibitor p27 Kip1 against atherosclerosis and restenosis, two disorders characterized by abundant proliferation and migration of vascular smooth muscle cells and adventitial fibroblasts. (
  • These therapeutic effects might result from p27 Kip1 -dependent suppression of both cell proliferation and migration. (
  • Moreover, a constitutively active mutant of the retinoblastoma protein (pRb) insensitive to CDK-dependent hyperphosphorylation inhibited both cell proliferation and migration. (
  • Collectively, these results suggest that cellular proliferation and migration are regulated in a coordinated manner by the p27 Kip1 /CDK/pRb pathway. (
  • Excessive proliferation and migration of vascular smooth muscle cells (VSMCs) and adventitial fibroblasts play an important role in the pathobiology of vascular occlusive disease (eg, atherosclerosis, in-stent restenosis, transplant vasculopathy, and vessel bypass graft failure). (
  • Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. (
  • JQ1 exhibits no adverse effects on proliferation, cell cycle and cell apoptosis of the normal human epithelial cells, while suppressed proliferation and cell cycle, and induced apoptosis of SACC cells, down-regulated the mRNA and protein levels of BRD4 in SACC cells, meanwhile reduced protein expressions of c-myc and BCL-2, two known target genes of BRD4. (
  • Moreover, PTEN overexpression induced cleavage of caspase-3 and significantly increased apoptosis compared with control cells. (
  • The effects of ferruginol on cell apoptosis, cell migration and cell cycle phase distribution were also evaluated. (
  • When cells were treated with 20, 80 and 300 µM ferruginol, cells began to exhibit yellow fluorescence, which indicated the onset of apoptosis. (
  • In conclusion, ferruginol may exhibit anticancer effects in OVCAR‑3 human ovary cancer cells by inducing apoptosis, inhibiting cancer cell migration and inducing G2/M cell and may therefore prove beneficial in the treatment and management of ovarian cancer. (
  • These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. (
  • GLPs have been demonstrated to induce suppression of cell growth and apoptosis in different types of cancer cells, with minimal negative effects on normal cells ( 13 ). (
  • Results: Exogenous overexpression of miR-623 suppressed cell proliferation, migration and invasion, meanwhile, but promoted cell apoptosis. (
  • No effects on mammary cancer cell proliferation or apoptosis were observed for either form of ADAMTS-15. (
  • Here, we review some recent progress in understanding the impact that PUFA may have on breast cancer cell proliferation, apoptosis, migration, and invasion. (
  • This paper aims to give a rapid overview of the effect of PUFAs on breast cancer cell proliferation, apoptosis, migration, and invasion. (
  • Activation of the p44/42 mitogen-activated protein kinase (MAPK) pathway plays a major role in regulating cell growth and survival in breast cancer cells [ 25 ] and is protective against apoptosis through phosphorylation of Bad [ 26 ]. (
  • DHA-induced apoptosis of breast cancer cells was also associated with up-regulation of the transmembrane heparan sulfate proteoglycan syndecan-1 [ 27 ]. (
  • Moreover increase of syndecan-1 impairs signaling of the MAPK pathway by inhibiting phosphorylation of MEK, Erk, and Bad, that results in apoptosis induction in breast cancer cells [ 28 ]. (
  • We found that Cdc42 is required for endothelial tip cell selection, directed cell migration and filopodia formation, but dispensable for cell proliferation or apoptosis. (
  • intimal thickening, inhibition of N-cadherin function by illness of human being saphenous vein sections with RAd dn-N-cadherin considerably decreased VSMC migration by 55% and improved VSMC apoptosis by 2.7-fold. (
  • Wnt signaling increases osteoprogenitor cell proliferation and prevents apoptosis ( 12 , 13 ). (
  • Further loss-of-function analysis revealed that knockdown of circVANGL1 inhibited proliferation and induced apoptosis in NSCLC cell lines. (
  • On the other hand, silencing of Gab1 accelerated apoptosis and repressed the growth of CS cells, which further caused the inhibition of G1/S phase transition and decreased invasion capacity in CS cell lines. (
  • Moreover, multi-target siRNA showed greater suppression effects on proliferation, migration, invasion, angiogenesis and induced apoptosis in HCC cells. (
  • Long non-coding RNA HOTAIR exerts regulatory functions in various biological processes in cancer cells, such as proliferation, apoptosis, mobility, and invasion. (
  • Targeting GLI1 expression in human inflammatory breast cancer cells enhances apoptosis and attenuates migration. (
  • X-linked inhibitor of apoptosis protein inhibition induces apoptosis and enhances chemotherapy sensitivity in human prostate cancer cells. (
  • Androgen-insensitive prostate cancer cells are highly resistant to several chemotherapeutic drugs and are characterized by the appearance of apoptosis-resistant cells. (
  • X-linked inhibitor of apoptosis protein inhibits apoptosis in inflammatory breast cancer cells with acquired resistance to an ErbB1/2 tyrosine kinase inhibitor. (
  • Tumor necrosis factor is a proinflammatory cytokine, which can target its two cognate receptors and initiate the activation of NF-κB, caspase, and the JNK pathways, leading to immune cell gene regulation, apoptosis, and/or their immune cell activation. (
  • Akt1 is a key player in PI3K-AktmTOR pathway that is vital for cell survival, proliferation, migration, invasion, metastasis, angiogenesis and apoptosis. (
  • Wound- healing assay and transwell assay were used to evaluate the activities of migration and invasion of SACC cells. (
  • Moreover, JQ1 inhibited SACC cell migration and invasion by regulating key epithelial-mesenchymal transition (EMT) characteristics including E-cadherin, Vimentin and Twist. (
  • Effects of triptolide on the proliferation and invasion of colon cancer cells and expression of cancer-related genes and proteins were assessed. (
  • Additionally, we show that triptolide treatment decreased expression of VEGF and COX-2, which promote cancer progression and invasion, and inhibited the expression of multiple cytokine receptors potentially involved in cell migration and cancer metastasis, including the thrombin receptor, CXCR4, TNF receptors, and TGF-β receptors. (
  • Antiproliferative Activity of Carnosic Acid is Mediated via Inhibition of Cell Migration and Invasion, and Suppression of Phosphatidylinositol 3-Kinases (PI3K)/AKT/Mammalian Target of Rapamycin (mTOR) Signaling Pathway. (
  • In addition, Vav1 also signals through Cdc42 to drive the formation of invadopodia and matrix degradation, which also promotes tumor cell invasion. (
  • Further, similar to siRNA-mediated depletion of Vav1, azathioprine treatment reduces cell migration, as well as the matrix degradation required for invasion. (
  • Taken together, these data uncover a role for aberrantly expressed Vav1 in regulation of the cytoskeletal machinery required for migration and invasion, and in the promotion and metastasis of pancreatic cancer. (
  • In SN50 group, both of neutrophils migration and macrophages invasion were less detected compared with control group. (
  • Li Q , Liu J , Jia Y , Li T , Zhang M , . miR-623 suppresses cell proliferation, migration and invasion through direct inhibition of XRCC5 in breast cancer. (
  • Conclusion: miR-623 suppressed cell proliferation, migration and invasion through downregulation of cyclin dependent kinases and inhibition of the phosphatidylinositol-3-kinase (PI3K)/Akt and Wnt/β-Catenin pathways by targeting XRCC5. (
  • Cell proliferation, migration and invasion were examined using CCK-8, colony formation and transwell assay. (
  • Numerous miRNA molecules have been shown to involved in various processes in tumorigenesis, including cancer cell proliferation, migration and invasion [ 11 - 13 ]. (
  • The effects of miR-625-3p in cell migration and invasion were analyzed by wound healing assay and transwell assay, respectively. (
  • In the present study, we found that overexpression of miR-625-3p promoted migration and invasion in SW480 cells, whereas downregulation of miR-625-3p inhibited cell motility in SW620 cells. (
  • More importantly, we observed potential binding sites for miR-625-3p in the 3′-untranslated region of suppressor of cancer cell invasion (SCAI). (
  • We found a greater decrease in cell survival, colony-forming ability, migration, and Cα OE cell invasion under glucose (Glc)-depletion conditions than under glutamine (Gln)-depletion conditions. (
  • Cancer cell migration and invasion increased due to LDHA elevation of the altered metabolic axis driven by activated CK2. (
  • FX11 treatment and LDHA knockdown suppressed migration and invasion through ROS generation, but this was partially reversed by the antioxidant N -acetylcysteine (NAC). (
  • CCK-8 assay, transwell migration and invasion assay were performed to investigate the effects of MALAT1 knockdown on the proliferation, migration and invasion of tongue cancer cells, respectively. (
  • MALAT1 knockdown markedly inhibits the proliferation, migration and invasion of tongue cancer cells. (
  • It showed no significant effects on Akt expression, while PI3K activator treatment reduced the inhibitory effects of MALAT1 knockdown on the proliferation, migration and invasion of tongue cancer cells. (
  • IMSEAR at SEARO: Inhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration. (
  • Osthole, an active constituent isolated from the dried C. monnieri fruit, has been shown to suppress tumor migration and invasion. (
  • Tumor invasion and metastasis are the main pathological characteristics of cancer cells, and mortality from cancer is primarily the result of metastatic spread to distant organs. (
  • Abnormal GBM biology leads to uncontrolled growth, invasion, and angiogenesis, which ultimately facilitates cell proliferation and negatively affects survival [ 2 - 6 ]. (
  • Among these hurdles is the aggressive local invasion of malignant cells from the original tumor into surrounding normal brain tissue, which makes complete surgical resection impossible. (
  • Finally, the roles of miR-203 in regulation of tumor proliferation, migration, invasion, and target gene expression were further investigated. (
  • Cell migration is involved in several pathological processes such as tumor invasion, neoangiogenesis and metastasis. (
  • Cell migration and invasion are live cell kinetic assays useful in oncology research. (
  • whereas invasion describes cells actively invading surrounding tissue. (
  • We hypothesized that galectin-3 may control the migration and invasion of cancer cells and that silencing of galectin-3 would therefore, suppress motility in osteosarcoma cells. (
  • Gal-3 expression in pancreatic cancer and gastric cancer cells increases cell migration and invasion ( 5 , 6 ). (
  • Overexpression of Gal-3 through the introduction of Gal-3 cDNA increases proliferation of oral tongue squamous cell carcinoma (TSCC) cells and induces invasion and epithelial-to-mesenchymal transition (EMT) ( 8 ). (
  • Emerging evidence demonstrates that microRNAs (miRNAs) play an important role in regulation of cell growth, invasion and metastasis through inhibiting the expression of their targets. (
  • It has been reported that miR-130a-3p controls cell growth, migration and invasion in a variety of cancer cells. (
  • The migration and invasion assays were also performed to explore the role of miR-130a-3p in GR HCC cells. (
  • Consistently, overexpression of miR-130a-3p or down-regulation of Smad4 suppressed the cell detachment, attachment, migration, and invasion in GR HCC cells. (
  • Altogether, KU-60019 inhibits the DNA damage response, reduces AKT phosphorylation and prosurvival signaling, inhibits migration and invasion, and effectively radiosensitizes human glioma cells. (
  • In pancreatic cancer, NUAK1 is specifically up-regulated and the down-regulation by miR-96 is capable of impeding the proliferation, migration and invasion of MIA PaCa-2 pancreatic cancer cells [ 15 ]. (
  • The aim of the present study was to determine whether activation of PAR1 or the other known PARs (PAR2-4) can regulate migration and invasion of breast cancer cells. (
  • Quite unexpectedly, we found that activation of PAR1 with thrombin or the peptide agonist SFLLRN markedly inhibited invasion and migration of MDAMB231 cells when applied as a concentration gradient in the direction of cell movement. (
  • Activation of G i signaling with epinephrine or wasp venom mastoparan also inhibited invasion and migration of the breast cancer cells. (
  • Because proteases are essential for cancer cells to invade through the ECM, there has been a concerted effort (13 , 14 , 19 , 20) to determine whether PARs are also involved in the invasion and metastasis processes. (
  • The migration and invasion of NSCLC cells were also suppressed by circVANGL1 knockdown. (
  • In this study, we aimed to investigate the role and molecular mechanism of HOTAIR in promoting HCC cell migration and invasion. (
  • In addition, knockdown of HOTAIR resulted in a decrease of cell migration and invasion, which could be specifically rescued by down-regulation of RBM38. (
  • Taken together, HOTAIR could promote migration and invasion of HCC cells by inhibiting RBM38, which indicated critical roles of HOTAIR and RBM38 in HCC progression. (
  • Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. (
  • The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western blot assay were performed to examine messenger RNA (mRNA) and protein levels in SACC cells. (
  • We have compared the capillary tube assay for migration inhibition studies with our modification of the agarose microdroplet technique, using several sources of factors with inhibitory activity (lymphokines, bacterial factors) and a variety of cell types (inflammatory exudate cells, tumor cells). (
  • Cell cytotoxicity induced by ferruginol was determined by an MTT assay, while fluorescence microscopy and transmission electron microscopy (TEM) were performed to investigate apoptotic effects. (
  • The percentage of migrated cells, determined by the wound healing assay, decreased from 98.7% in control to 68.2% and 45.3 in 80 and 300 µM ferruginol‑treated cells, respectively. (
  • Results of the reporter assay revealed that the luciferase activity was decreased in XRCC5-wt cells, suggesting that miR-623 could directly combine with 3' UTR of XRCC5. (
  • Using [email protected] NP stimulation and a transwell migration assay, we found that the migration of HeLa cells was significantly decreased. (
  • In contrast to its effects on cell migration, wt ADAMTS-15 but not the E362A inactive mutant inhibited endothelial tubulogenesis in 3D collagen gels and angiogenesis in the aortic ring assay. (
  • An experimentally accessible example of multi-cell migration is provided by the classic scratch-wound assay. (
  • In this assay, a confluent monolayer is `injured' by forcibly removing a strip of cells, and the remaining monolayer `heals' through some combination of cell migration, spreading and proliferation. (
  • The scratch wound has been used for decades as a model of wound healing and an assay of cell migration , however the mechanisms that underlie the coherent expansion of cells in the surviving monolayer are still debated. (
  • In this assay, contact-inhibited cells are grown on a 2D surface until they form a confluent monolayer. (
  • Although the scratch-wound assay has been used to help identify many molecules and signaling pathways important for cell migration, surprisingly few studies have attempted to reveal the impetus for the healing response. (
  • support the idea that the release of spatial constraints can initiate the healing response, but they do not address the mechanism(s) that sustain collective migration in the scratch-wound assay. (
  • Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points. (
  • Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury. (
  • In addition, the A549 lung adenocarcinoma cell line was characterized for cell proliferation using the MTT assay and cell migration using the scratch migration assay. (
  • The wound healing assay was conducted to determine the cell migratory activity in GR HCC cells treated with miR-130a-3p mimics. (
  • In contrast, phenotype-driven screens have shown a much stronger success rate, which is why we developed an in vivo assay using transgenic zebrafish with a GFP-marked migrating posterior lateral line primordium (PLLp) to identify compounds that influence collective cell migration. (
  • The effect of siRNA on cell proliferation and invasiveness was assessed by cell counting kit-8 ( CCK-8 ) assay, Transwell migration assay and wound healing assay. (
  • To analyze the effect of VEGF and anti-Flt-1 peptide on the migration of VSMCs under a high glucose condition, transwell assay with a matrigel filter was performed. (
  • PTEN also has sequence similarity to tensin ( 1 ), a cytoskeletal protein that binds to actin filaments at focal adhesions and is tyrosine phosphorylated upon integrin-mediated cell adhesion ( 6 ). (
  • Inhibition of cell-matrix adhesion may be a plausible mechanism by which this progression is occurring. (
  • Focal adhesion kinase (FAK) is important for the metastasis of cancer cells. (
  • Tumor metastasis is a highly complex multistep process that includes changes in cell-cell adhesion properties [ 1 ]. (
  • Conclusions Beneath the condition of the research, cell-cell adhesion mediated by N-cadherin regulates VSMC migration via modulation of viability. (
  • In response to extracellular cues, the cell initiates the motility by setting up a front-to-back polarization, followed by a coordinated cycle of actin polymerization-dependent protrusion, integrin/actin-mediated focal adhesion and cell body translocation resulting from actomyosin contractility, which finally leads to the cell movement [ 9 ]. (
  • Galectin-3 is involved in tumor cell proliferation, adhesion, angiogenesis and metastasis. (
  • Gal-3 in breast cancer cells greatly enhances adhesion to endothelial cells ( 7 ). (
  • In all cases, we observed a greatly enhanced α2β1 integrin-dependent cell migration associated with focal adhesion rearrangements and increased outside-in signaling as demonstrated by elevated phosphorylation of focal adhesion kinase and MAPKinase (ERK1 and ERK2). (
  • Moreover, over-expression of Thy-1 by transfection of HUVEC with Thy-1 pcDNA3.1 decreased the activity of RhoA and Rac-1 and inhibited the adhesion, migration and capillary-like tube formation of these cells. (
  • For instance, studies (21) have shown that thrombin activation of PAR1 promotes cell adhesion to vitronectin, whereas trypsin activation of PAR2 stimulates α 5 β 1 -dependent adhesion to fibronectin in human gastric carcinoma cells. (
  • The expression of junctional adhesion molecule C in endothelial cells is required to prevent reverse transendothelial migration of neutrophils. (
  • May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system. (
  • Inhibition of Rho kinase (ROCK) further reduced intercellular adhesion on apical and lateral surfaces but did not disrupt basal tissue organization. (
  • Cell migration and adhesion are critical for immune system function and involve many proteins, which must be continuously transported and recycled in the cell. (
  • Cell migration and cell adhesion are critical processes in cells of the immune system. (
  • These phenomena require cell adhesion and cell migration. (
  • During migration, the adhesion proteins function as traction points that drive the formation and stabilization of membrane projections, thus allowing the movement of cells ( 2 ). (
  • 16971514 ). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. (
  • Cells can migrate as a cohesive group (e.g. epithelial cells) or have transient cell-cell adhesion sites (e.g. mesenchymal cells). (
  • Using these adhesion sites, cells also sense the mechanical properties of the ECM. (
  • Bves is a highly conserved, transmembrane protein that is involved in cell adhesion, cell motility, and most recently has been shown to play a role in vesicular transport. (
  • Grossly, cell motility and cell adhesion are impaired. (
  • Upon Bves disruption, cell rounding is increased, a phenotype indicative of decreased adhesion and disruption of integrin function. (
  • Time-lapse video microscopy during patch-clamp recordings revealed visible changes in cell shape and/or movement that accompanied spontaneous activation of I Cl,Vol , suggesting that I Cl,Vol is activated during cell movement, consistent with the effects of NPPB in migration assays. (
  • In experimental metastasis assays in nude mice, MDA-MB-231 cells expressing either form of ADAMTS-15 showed reduced spread to the liver, though lung colonization was enhanced for cells expressing wt ADAMTS-15. (
  • Cells mobility was detected by wound healing and Transwell assays. (
  • The authors assessed the epithelial cells' ability to migrate and proliferate using cell plating and proliferation assays (optical densitometry and immunofluorescence (IF) of Ki67, a proliferation marker), respectively. (
  • Results from this study show that incubating GBM cells with osthole reduces matrix metalloproteinase (MMP)-13 expression and cell motility, as assessed by cell transwell and wound healing assays. (
  • Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. (
  • Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis. (
  • There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models. (
  • Selected compounds were confirmed in their migration-blocking activity by using additional assays for cell migration. (
  • These results suggest that GABA, a single ligand, can promote motility via G-protein activation and arrest attractant-induced migration via GABA A receptor-mediated depolarization. (
  • Micromolar concentrations stimulate chemokinesis (random motility), whereas femtomolar GABA levels induce chemotaxis (directed migration). (
  • In contrast, the population exhibiting chemotaxis to femtomolar GABA is not enriched in GAD + cells, indicating that each GABA concentration stimulates motility in a distinct subpopulation of cortical neurons. (
  • Vz and cp cell motility responses to each chemotropic concentration of GABA were analyzed. (
  • However, both forms reduced cell migration on fibronectin or laminin matrices, though motility on a Type I collagen matrix was unimpaired. (
  • This study also provides evidence supporting the potential of osthole in reducing FAK activation, MMP-13 expression, and cell motility in human GBM cells. (
  • Application of specific target gene siRNA (ERα, ERβ, p38α, and p38β) to LoVo cells further confirmed that p38 MAPK mediates E 2 /ERs inhibition of MMP-2 and -9 expression and cell motility in LoVo cells. (
  • This kinase is thought to stimulate cell motility by promoting turnover of focal contacts and is overexpressed in ovarian cancers ( 6 ). (
  • Cells impinge force on their extracellular environments during tissue morphogenesis, cardiovascular and pulmonary function, directed cell motility, and the immune response. (
  • Studies found that cortactin could bind to Arp2/3, and influence cell motility, it also was considered has a relationship with cell migration [ 18 ]. (
  • The ability of cells to generate polarized distributions of several molecules enables numerous biological process including asymmetric cell division, cell motility, and formation of the immunological synapse. (
  • Bves interacts with GEFT, a protein that modulates Rho GTPases, Rac1 and Cdc42, which are important for cell motility through modulation of the actin cytoskeleton. (
  • Also, the results indicated that even though the migration inhibition was closely related to SRC and FAK signaling pathway, there may be another unknown regulation mechanism existing and its metastasis inhibition was significant. (
  • Collectively, these results suggest that E 2 treatment down-regulates cell proliferation by modulating the expression of cyclin A, cyclin D1 and cyclin E. E 2 treatment simultaneously impaired cell migration by inhibiting the expression of uPA, tPA, MMP-2, and MMP-9 through E 2 /ERs-p38α MAPK signaling pathway in human LoVo colon cancer cells. (
  • Inhibition of the Akt signaling pathway significantly decreases Th17 cell migration. (
  • Metalloproteinase-dependent and -independent processes contribute to inhibition of breast cancer cell migration, angiogenesis and liver metastasis by a disintegrin and metalloproteinase with thrombospondin motifs-15. (
  • An example is angiogenesis - the process of new blood vessel formation from existing ones - and the role of Cdc42 in endothelial cells (ECs). (
  • Vascular endothelial growth factor (VEGF) is a well-known gene in cancer therapy researches, angiogenesis inhibition is considered as an effective way for tumor treatment, thus VEGF is a good target for cancer therapy [ 24 - 26 ]. (
  • Vascular endothelial growth factor (VEGF) increases angiogenesis by stimulating endothelial cell (EC) migration. (
  • V ascular endothelial growth factor (VEGF) is a potent stimulus of endothelial cell (EC) migration and angiogenesis. (
  • Endothelial cell (EC) migration is required for both physiological and pathological angiogenesis. (
  • BRD4 knockdown cells are growth impaired and grow more slowly than control cells accompanied by decrease in G1 gene expression [ 15 ]. (
  • Knockdown of syndecan-4 attenuated the inhibitory effects of ADAMTS-15 on cell migration. (
  • Treatment with MK571, or specific MRP1 knockdown, did not have any effect on temozolomide drug response in these cells. (
  • Knockdown (KD) of CXCR3 in metastatic tumor cells suppressed tumor cell migration and metastasis. (
  • Interestingly, compared to control cells, the pharmacological inhibition of PI3Kinase or the siRNA-mediated knockdown of AKT had little effect on the high α2β1-mediated cell migration observed in the absence of αv integrins or following activation of α2β1 integrins by the TS2/16. (
  • For example, NUAK1 is highly expressed in non-small cell lung cancer (NSCLC) and knockdown of this gene served as a block in lung metastasis and invasive ability in a xenograft mouse model [ 8 ]. (
  • Rescue experiments demonstrated that miR-195 inhibitor abrogated the beneficial role of circVANGL1 knockdown in NSCLC cells. (
  • These effects are nearly eliminated in atRA-treated shClmn knockdown cells. (
  • NO stimulated endoplasmic reticulum (ER) 45 Ca 2+ uptake, a measure of SERCA activity, and knockdown of SERCA2 prevented VEGF-induced EC migration and 45 Ca 2+ uptake. (
  • 12 The predominant 3-phosphoinositide lipid products of PI 3-kinase, PI 3,4-bisphosphate and PI 3,4,5-trisphosphate (PIP3), are potent signaling molecules that regulate numerous cellular processes required for neointimal hyperplasia and restenosis, including cell proliferation, migration, and survival. (
  • Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. (
  • Nivolumab is a fully human, immunoglobulin G4 (kappa) isotype monoclonal antibody that binds programmed cell death protein 1 (PD-1) on activated immune cells and disrupts the engagement of the receptor with its ligands programmed death-ligand 1 (PD-L1: B7-H1/CD274 and PD-L2: B7-DC/CD273), thereby abrogating inhibitory signals and augmenting the antitumor response of the host [1]. (
  • Pertussis toxin (PTX) blocked GABA-induced migration, indicating that chemotropic signals involve G-protein activation. (
  • We investigated the intracellular metabolic fluxes of protein kinase CK2-activating (Cα OE) cells and role of lactate dehydrogenase A (LDHA) as a contributor of tumorigenesis after reprogrammed glucose metabolism. (
  • We have explored the effects of A Disintegrin and Metalloproteinase with Thrombospondin motifs-15 (ADAMTS-15) on the behavior of MDA-MB-231 and MCF-7 breast cancer cells by stable expression of either a wild-type (wt) or metalloproteinase-inactive (E362A) protein. (
  • 2. IF for the cytoskeletal protein phalloidin was completed to assess for effect of Notch inhibition on the cell cytoskeleton. (
  • The presence of MK571 (inhibitor of MRP1 and multidrug resistance protein 4 (MRP4) led to an enhanced effect of vincristine and etoposide in reducing cell viability over a 72 h period. (
  • With this study, we've demonstrated that inhibition from the function of N-cadherin (a cell-cell get in touch with protein) considerably retards Triptonide supplier VSMC migration and intimal thickening, while advertising endothelial protection, and may consequently be clinically helpful for dealing with intimal thickening. (
  • Homoharringtonine (protein biosynthesis inhibitor) and doxorubicin significantly inhibited cell migration, however, they did not exert obvious effects on microtubules. (
  • p90RSK, OPN, GSK-3β protein expression were examined in the A549 human lung adenocarcinoma cell line in the presence and absence of BI-D1870 (BID), a p90RSK inhibitor. (
  • In contrast, treatment with interleukin-1β (IL-1β) or phorbol-12-myristate-13-acetate (PMA) increased the level of Thy-1 protein and reduced the migration of HUVEC. (
  • What we have shown,¿ he went on, ¿is that the Slit protein also affects immune system cell types, including leukocytes, lymphocytes, monocytes and macrophages. (
  • In this study, we found that myosin VI, a motor protein that regulates border cell migration, is abundantly expressed in high-grade ovarian carcinomas but not in normal ovary and ovarian cancers that behave indolently. (
  • Geisbrecht and Montell ( 11 ) reported that the actin-based motor protein myosin VI is highly expressed in migrating border cells, and that depleting myosin VI from border cells severely retards their migration. (
  • Bioinformatics analysis was conducted to screen differentially expressed genes in CCA, Western blot analysis detected NUAK1 protein expression and RT-qPCR detected miR-1182, let-7a and NUAK1 expression in CCA tissues and cell lines. (
  • Modern immunotherapy with immunomodulating antibodies, dubbed immune checkpoint inhibitors, such as anti-programmed cell death protein- 1 (anti-PD- 1 ) inhibitors nivolumab and pembrolizumab, showed unprecedented activity in one first-line (KEYNOTE-048) and several second-line trials (CheckMate-141, KEYNOTE-012, KEYNOTE-055, and KEYNOTE-040). (
  • Visit the Cell Migration Knowledgebase for the latest cell migration protein database entries. (
  • The basolateral marker scribble and the apical marker atypical protein kinase C zeta localized to all interior cell membranes, whereas PAR3 displayed a cytoplasmic localization, suggesting that the apico-basal polarity was incomplete. (
  • HOTAIR suppression using RNAi strategy with HepG2 and Bel-7402 cells increased the mRNA and protein expression levels of RNA binding motif protein 38 (RBM38). (
  • These sphingolipid- and cholesterol-enriched lipid microdomains (lipid rafts) recruit several protein components and compartmentalize cellular processes associated with cell signaling and membrane trafficking ( 4 ). (
  • NO)‐dependent signaling is required for vascular endothelial growth factor (VEGF)‐induced EC migration, but the protein targets that may be redox regulated are poorly understood. (
  • In TNF-treated cells, TNFR1, TNFR-associated death domain protein (TRADD), Fas-associated death domain protein, and receptor-interacting protein kinase proteins form the signaling complex via modular interaction within their C-terminal death domains. (
  • By using adenoviral-mediated expression of inducible heme oxygenase 1 in primary vascular smooth muscle cells (vsmc) in vivo, the present inventors demonstrate that in vivo expression of HO1 can be used to treat restenosis. (
  • In vivo , low concentrations of attractants would stimulate cells to begin migrating. (
  • Furthermore, we evaluated whether inhibition of N-cadherin function retarded intimal thickening by modulation of VSMC migration and success using an ex lover vivo human being saphenous vein style of intimal thickening. (
  • We identified 165 compounds that interfere with primordium migration without overt toxicity in vivo . (
  • In vivo migration of granulocytes was decreased in patients with atopic asthma and elevated levels of IgE as well as in patients with infectious asthma and elevated levels of IgG. (
  • In vivo migration of granulocytes showed significant decreases in both asthmatic groups only during asthmatic attacks. (
  • His team has contributed to the screening packages of several novel anti-cancer compounds which have subsequently progressed to clinical trials and beyond, using both cell-based and in vivo technologies. (
  • Synergism from combined immunologic and pharmacologic inhibition of HER2 in vivo. (
  • Confinement is also observed in vivo, where the optimal width is a function of the number of migrating cells in different streams of different species. (
  • As heparan sulfate proteoglycans (HSPGs) are known as co-receptors to interact with numerous growth factors and then modulate downstream biological activities, overexpression of HS/HSPG on cell surface acts as an increasingly reliable prognostic factor in tumor progression. (
  • Interaction between cell surface and microenvironment plays a crucial role in malignant tumor progression. (
  • It has been reported that BRD4 is associated with a variety of cancers due to its role in the regulation of cell cycle progression [ 7 - 12 ]. (
  • Further study indicates that BRD4 has a vital role in promoting cell cycle progression from G0 to G1 and entry into S phase. (
  • This work demonstrated that vWF, as a downstream effector of miR-24 , played an important role in controlling OS cell progression. (
  • The metastasis of cancer cells is a vital aspect of disease progression and therapy. (
  • To investigate the role of p90RSK in lung adenocarcinomas and whether the inhibition of p90RSK diminishes cancer progression. (
  • Several genes that control border cell migration are homologous to human genes that promote ovarian cancer progression. (
  • Given the similarities at the molecular and behavioral levels between border cell migration and ovarian cancer progression, studying human homologs of other Drosophila genes that control border cell migration could provide new insight into the migratory behavior of ovarian cancer cells. (
  • This study supports the validity of a "cross-species" approach of using genetic analysis of border cell migration in Drosophila to identify novel mediators of ovarian cancer progression. (
  • These findings suggest that therapeutics targeted toward G i -couplers that are selectively expressed in breast cancer cells could prove beneficial in halting the progression of invasive breast cancer. (
  • Gab1 but not Grb2mediates tumor progression in Met overexpressing colorectal cancer cells. (
  • Cell cycle progression is controlled by several cyclin-dependent kinases (CDKs) that associate with regulatory cyclins. (
  • Triptolide inhibited mRNA expression of the positive cell cycle regulatory genes c-myc, and A, B, C, and D-type cyclins in multiple colon cancer cell lines. (
  • It has been known that miRNAs exert their regulatory functions through binding to the 3′ untranslated region (3′UTR) of target mRNA, leading to the degradation of the mRNA or translational inhibition of functional proteins. (
  • Anti-Flt-1 peptide also inhibits the VSMCs' migration and the expressions of MMP-3 and MMP-9 mRNA induced by VEGF under a high glucose condition. (
  • Neuroblastoma cells (SH-SY5Y and Neuro2a or N2A) exposed to atRA undergo growth inhibition and neuronal differentiation, both of which are preceded by an increase in Clmn mRNA. (
  • Studies with cultured human cells have found that there are a large number of ALOX5 mRNA splice variants due to Alternative splicing . (
  • On cell surface negatively charged HS provides the initial attachment of basic CPPs by electrostatic interaction, leading to multiple cellular effects. (
  • Phosphatidylinositol (PI) 3-kinase signaling regulates numerous cellular processes, including proliferation, migration, and survival, which are required for neointimal hyperplasia and restenosis. (
  • To better understand the impact of this regulation on cellular activity, cell model systems were devised in which SERCA is mutated at cysteine-674 to serine, thus lacking the reactive thiol. (
  • Cellular migration is impaired by juglone/ascorbate. (
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (
  • However, the molecular and cellular mechanisms supporting the effect of PUFAs in breast cancer cells remain relatively unknown. (
  • Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. (
  • At the cellular level, A-T cells are extremely sensitive to ionizing radiation, have impaired G 1 -S, intra-S, and G 2 -M checkpoints, and show elevated levels of chromosomal instability ( 4 ). (
  • At 14 days after balloon injury , the neointimal proliferation and vascular luminal stenosis were measured, and cellular proliferation was assessed by counting the proliferative cell nuclear antigen ( PCNA ) stained cells . (
  • We found that signaling mediated by the Eph family of receptor tyrosine kinases from cell-cell contacts changed the cellular response to the growth factor EGF by modulating the vesicular trafficking of its receptor, EGFR. (
  • We show here that p27 Kip1 inhibits cellular changes that normally occur during cell locomotion (eg, lamellipodia formation and reorganization of actin filaments and focal adhesions). (
  • In summary, our data emphasize the importance of miR301 inhibition on PI3K-Akt pathway-mediated cellular functions. (
  • The proto-oncogene Vav1 is ectopically expressed in over half of human pancreatic cancers, and its expression correlates with a poor prognosis in humans and promotes survival and transformation in isolated tumor cells. (
  • We have found that Vav1, through its GEF activity towards Rac1, also potently induces migration by pancreatic tumor cells. (
  • Vav1-expressing tumor cells were more sensitive to azathioprine than Vav1-negative cells, supporting the premise that the inhibitory effects of azathioprine are mediated through Vav1. (
  • Even with the expression of multiple Rho family GEFs, tumor cells that upregulate Vav1 have become dependent upon this exchange factor, implicating Vav1 as a potent node for therapeutic intervention. (
  • We observed an increased expression of CXCR3 in metastatic tumor cells compared to those from non-metastatic tumor cells. (
  • On the other hand, CXCR3+ NK cells infiltrated tumor cells in murine lymphoma and melanoma versions inside a CXCL10-reliant way (33). (
  • Here we report that SASP from senescent stromal fibroblasts promote spontaneous morphological changes accompanied by an aggressive migratory behavior in originally non-motile human breast cancer cells. (
  • SASP-induced morphological/migratory changes are critically dependent on microtubule integrity and dynamics, and are coordinated by the inhibition of RhoA and cell contractility. (
  • 1. Decreased Notch signaling increases the migratory capacity, but not proliferation, of corneal epithelial cells and improves healing of corneal wounds. (
  • Inhibition of the Notch pathway, either by direct inhibition using DAPT or Notch1 knockout using shRNA, results in a pro-migratory phase for corneal epithelium especially near the leading edge. (
  • During normal development of the Drosophila ovary, a dynamic process called border cell migration occurs that resembles the migratory behavior of human ovarian cancer cells. (
  • Eph receptor activation prevented the recycling of EGFR to the cell surface (the subcellular compartment from where it mediates migratory signaling) by trapping EGFR in endosomes (the subcellular compartment from where it can continue to promote proliferative signaling). (
  • Single-cell labeling revealed that individual cells were both protrusive and migratory within the epithelial multilayer. (
  • Akt-dependent enhanced migratory capacity of Th17 cells from children with lupus nephritis. (
  • Th17 cells from children with LN exhibit high Akt activity and enhanced migratory capacity. (
  • GLPs have been demonstrated to be able to directly induce cell differentiation of leukemia cells by caspase cleavage and p53 activation ( 18 ). (
  • miRNAs are involved in a wide range of biological processes including cell death, cell proliferation, cell division, differentiation and tumorigenesis [ 7 - 10 ]. (
  • When the epithelium is injured, remaining epithelial cells attempt to correct the defect rapidly, hypothesized to occur by proliferation and differentiation. (
  • The vitamin A metabolite all-trans retinoic acid (atRA) functions in nervous system development and regulates cell proliferation and differentiation. (
  • Border cells in flies (Drosophila melanogaster): the border cells migrate during the differentiation of egg cells to be ready for fertilization. (
  • abstract = "Cells induced into senescence exhibit a marked increase in the secretion of pro-inflammatory cytokines termed senescence-associated secretory phenotype (SASP). (
  • Oddly enough, inhibition of N-cadherin function considerably retards intimal thickening via inhibition of VSMC migration and advertising of endothelial cell success. (
  • On the other hand, pre-treatment with a ROCK (a kinase associated with RhoA for transducing RhoA signaling) inhibitor, Y27632, abolished the RhoA V14-induced prevention effect on the Thy-1-induced inhibition of endothelial cell migration and tube formation. (
  • In the present study, we uncover a completely novel role of Thy-1 in endothelial cell behaviors. (
  • Gal-3 has been shown to be involved in extracellular interactions between the cell surface and extracellular matrix glycoproteins and glycolipids, and intracellular interactions between nuclear and cytoplasmic proteins to regulate signaling pathways ( 2 ). (
  • Different ECM proteins (such as collagen, elastin, fibronectin, laminin, and others) allow cells to adhere and migrate, while forming focal adhesions in the front and disassembling them in the back. (
  • Cells can be guided by a gradient of those proteins (haptotaxis) or a gradient of soluble substrates in the liquid phase surrounding the cell (chemotaxis). (
  • The contacts (cell-junctions) are created by transmembrane glycoproteins named cadherins (E-cadherin, N-cadherin or cadherin 11) and other proteins. (
  • To study the direct influence of NPs on cancer metastasis, the potential suppression capacity of [email protected] NPs to tumor cell migration, a kind of typical photothermal NPs, was systemically evaluated in this study. (
  • IFN-γ neutralization diminished the metastasis inhibition in the CXCR3 knockout (KO) mice bearing 4T1 tumors, suggesting a critical role of host CXCR3 in immune suppression. (
  • Taken together, these results indicate that suppression of the RhoA-mediated pathway might participate in the Thy-1-induced migration inhibition in HUVEC. (
  • Overexpression of miR-623 resulted in the downregulation of CDK4/6 as well as the inhibition of the phosphatidylinositol-3-kinase (PI3K)/Akt and Wnt/β-Catenin signaling pathways. (
  • In cultured A431 cells, N-benzyl-ACM and N-allyl-ACM also blocked both the membrane localization of H-Ras and activation of the H-Ras-dependent PI3K/Akt pathway. (
  • Thus, N-benzyl-ACM and N-allyl-ACM inhibited EGF-induced migration of A431 cells by inhibiting the farnesylation of H-Ras and subsequent H-Ras-dependent activation of the PI3K/Akt pathway. (
  • Overexpression of Gab1 increased Bcl-2/BAX ratio to promote cell growth via PI3K/AKT. (
  • In this study, we show that miR301 inhibition influences PI3K-Akt pathway activity. (
  • Recently, the anti-inflammatory agent azathioprine was described as an inhibitor of Vav1/Rac1 activity in hematopoietic cells. (
  • 1. Inhibition of Notch signaling was induced in human corneal epithelial cells, both via exposure to DAPT, a Notch inhibitor, and via knockout of the Notch1 gene using stable transfection with Notch1-shorthairpin RNA (shRNA). (
  • The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration. (
  • Pre-treatment with Reversan (inhibitor of MRP1 and P-glycoprotein) led to a significantly improved response to cell death in the presence of all three chemotherapeutics, in both primary and recurrent GBM cells. (
  • After administration of inhibitors including QNZ (NFκB inhibitor), LY294002 (Akt activation inhibitor), U0126 (ERK1/2 inhibitor), SB203580 (p38 MAPK inhibitor) or SP600125 (JNK1/2 inhibitor), E 2 -downregulated cell migration and expression of MMP-2 and MMP-9 in LoVo cells is markedly inhibited only by p38 MAPK inhibitors, SB203580. (
  • Recently, a specific inhibitor of the ATM kinase, KU-55933, was shown to radiosensitize human cancer cells. (
  • In line with this finding, the effect of KU-60019 on AKT phosphorylation was countered by low levels of okadaic acid, a phosphatase inhibitor, and A-T cells were impaired in S473 AKT phosphorylation in response to radiation and insulin and unresponsive to KU-60019. (
  • We demonstrated that the Src inhibitor SU6656, identified in our screen, can be used to suppress the metastatic capacity of a highly aggressive mammary tumor cell line. (
  • Conclusions: These data suggest that preserving the NO signal through endogenous inhibition of asymmetric dimethylarginine (the endogenous nitric oxide inhibitor) enhances wound repair even in the presence of swine dust exposure. (
  • The team then inhibited PAK4 in cell lines by either using a drug inhibitor or a gene editing technique called CRISPR-Cas9. (
  • Cells overexpressing miR301-inhibitor and Akt, exhibited increased migration and proliferation. (
  • However, it is unclear whether miR-130a-3p regulates epithelial-mesenchymal transition (EMT) in drug resistant cancer cells. (
  • Because border cells behave like cancer cells, we investigated whether myosin VI regulates ovarian cancer cell migration. (
  • Additionally, GLPs may suppress Hep2 cells via the regulation of hepatic miRNAs and immune-associated miRNAs ( 15 ), and may reverse drug resistance by inhibition of the expression of resistance genes in the SKOV-3/DDP resistant ovarian cancer cell line ( 19 ). (
  • The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. (
  • Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants. (
  • This similarity between the immune and nervous systems,¿ Rao continued, ¿implies new therapeutic approaches to treat diseases that involve leukocyte migration to various areas of the body. (
  • What we are showing here is that they also regulate migration of immune system white blood cells, which are expressed in different regions of the body. (
  • Collectively, these data underline not only the importance of the CXCR3 axis in recruitment of effector immune cells, but also reveal complex relationships of receptor-ligand interactions in a TME-specific context. (
  • Newswise - Los Angeles - Cancer immunology drugs, which harness the body's immune system to better attack cancer cells, have significantly changed the face of cancer treatment. (
  • The new research from UCLA demonstrates that high expression of this oncogene also correlates with a lack of immune cells migrating into the tumors to destroy the cancer cells. (
  • They saw that the tumors that did not respond to PD-1 blockade had a high expression of PAK4 and were not infiltrated by immune cells, meaning that the immune cells had not found their way to the tumor to attack the cancer cells. (
  • The scientists found that deleting PAK4 increased the migration of tumor-specific immune cells and sensitized tumors to PD-1 blockade immunotherapy, reversing the resistance. (
  • Inhibition of lens epithelial cell migration by acrylic intraocular lens: New evaluation method of optic edge sharpness with quantification of edge curvature. (
  • However, the directional migration also requires the intact microtubule cytoskeleton. (
  • Tension driving FA maturation can be supplied either by myosin II forces transmitted to FAs through the actin cytoskeleton or by external forces applied to the cell. (
  • The UCLA study , published in the inaugural issue of the new scientific journal Nature Cancer, showed that genetic and pharmacological inhibition of the oncogene PAK4 overcomes resistance to anti-PD-1 therapy in preclinical models. (
  • Influence of route and dose of antigen on the migration inhibition and plaque-forming cell responses to sheep erythrocytes in the lizard, Calotes versicolor. (
  • Serum from immunized guinea pigs increases the inhibitory effect of antigen on the migration of cells from sensitive tissue and confers antigenspecific sensitivity on normal cells. (
  • Binding of canonical Wnts to frizzled (Fz) receptor and low-density lipoprotein 5 or 6 (LRP5/6) co-receptors leads to inhibition of β-catenin phosphorylation and subsequent translocation into the nucleus and activates the expression of Wnt-responsive genes ( 11 ). (
  • Abnormal expression of cell surface molecules notably contributes to enhance tumor cell growth, survival, migration, and invasiveness [ 4 ]. (
  • Because Vav1 is ectopically expressed in pancreatic cancers and is a potent regulator of tumor cell survival, proliferation, and invasive migration, it could provide a specific therapeutic target for these tumors. (
  • Confirmed by long-term survival curve study, [email protected] NPs significantly reduced the metastasis of cancer cells and improved the survival rates of metastasis in a mouse model. (
  • As a result, much work has centered on identifying the molecular targets involved in the tumor cell proliferation, survival and metastasis in effort to identify novel therapeutic approaches. (
  • A microdissection technique was used to separate differentiated cortical plate (cp) cells from immature ventricular zone cells (vz) in the rat embryonic cortex. (
  • Eph or Kiss1R activation also suppressed EGF-promoted migration in Pten −/− mouse embryonic fibroblasts, which exhibit increased constitutive Akt activity, and in MDA-MB-231 triple-negative breast cancer cells, which overexpress EGFR. (
  • Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors. (
  • Human embryonic kidney cells (HEK293), human HCC cells (MHCC97H, MHCC97L, HepG2, SMMC-7721) and human normal liver cells (LO2) were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10 % fetal bovine serum (FBS, Gibco), 2 mM L-glutamine, 100 U/ml of penicillin and 100 μg/ml of streptomycin. (
  • The epithelium internalizes and elongates into the embryonic mesenchyme as a solid, multilayered cord of cells from embryonic days 12-15. (
  • Collective cell migration is an essential process in the lives of multicellular organisms, e.g. embryonic development, wound healing and cancer spreading (metastasis). (
  • Overexpression of PTEN inhibited cell migration, whereas antisense PTEN enhanced migration. (
  • Effects of PTEN were assessed in primary rabbit VSMCs after overexpression with a recombinant adenovirus and compared with uninfected or control virus-infected cells. (
  • Methods and Results- To test our hypothesis, overexpression of either wild type (WT) or mutant SERCA in which cysteine-674 was mutated to serine was accomplished by stable transfection of HEK 293 or adenoviral expression in rat aortic smooth muscle cells (VSMCs). (
  • However, the migration-inhibiting activity of miR-24 was predominantly attenuated by vWF overexpression. (
  • Furthermore, overexpression of miR-130a-3p suppressed Smad4 expression, whereas inhibition of miR-130a-3p increased Smad4 expression. (
  • Akt overexpression in MCF7 and MDAMB468 cells caused downregulation of miR301 expression. (
  • Conclusions- In cells overexpressing SERCA, the cyclic GMP-independent, redox regulation of SERCA cysteine-674 is required for the inhibition of cell migration by both exogenous and endogenously generated NO. (
  • We investigated whether PTEN participates in cell migration, growth, spreading, and cytoskeletal regulation through transfection experiments with four cell types. (
  • The down-regulation of multiple cytokine receptors, in combination with inhibition of COX-2 and VEGF and positive cell cycle regulators, may contribute to the antimetastatic action of this herbal extract. (
  • Because Ca 2+ is an important second messenger for cell migration, we hypothesized that NO also inhibits cell migration through redox regulation of SERCA activity via cysteine-674. (
  • Although redox regulation of SERCA by S -glutathiolation may cause acute cyclic GMP-independent arterial relaxation, it is unknown if redox-dependent regulation of SERCA is involved in more chronic effects of NO such as those on cell migration. (
  • We demonstrated in this study that C674 is required for NO-mediated stimulation of SERCA activity and inhibition of Ca 2+ influx in cells, and that this regulation is essential for NO-induced inhibition of Ca 2+ influx and cell migration. (
  • We found the down-regulation of miR-130a-3p in GR HCC cells. (
  • For example, upregulated miR-182 increases drug resistance in cisplatin-treated HCC cells through regulation of TP53INP1 [ 10 ]. (
  • Crosstalk between integrins is involved in the regulation of various cell functions including cell migration. (
  • These results suggest that integrins αvβ5/β6 repress α2β1 possibly by interfering with their activation process and thereby modify the cell signaling regulation of α2β1-mediated migration. (
  • Up-regulation of urokinase plasminogen activator (uPA), tissue plasminogen activator (tPA), and matrix metallopeptidases (MMPs) is reported to associate with the development of cancer cell mobility, metastasis, and subsequent malignant tumor. (
  • Ibuprofen inhibition of tendon cell migration and down-regulation of paxillin expression. (
  • In conclusion, ibuprofen inhibits tendon cell migration in a process that is probably mediated by the down-regulation of paxillin. (
  • NO-mediated redox regulation of the sarco/endoplasmic reticulum Ca 2+ ATPase (SERCA) in VEGF-induced signaling and EC migration. (
  • NO-mediated activation of SERCA2b via S -glutathiolation of cysteine-674 is required for VEGF-induced EC Ca 2+ influx and migration, and establish redox regulation of SERCA2b as a key component in angiogenic signaling. (
  • HS proteoglycan (HSPG) present in the extracellular matrix (ECM) provides structural frameworks to mediate cell-cell communication and function in growth factor-receptor binding [ 5 , 6 ]. (
  • Thus, PTEN phosphatase may function as a tumor suppressor by negatively regulating cell interactions with the extracellular matrix. (
  • The sarco/endoplasmic reticulum calcium ATPase (SERCA) lowers cell Ca 2+ by increasing intracellular Ca 2+ uptake and inhibiting extracellular Ca 2+ influx. (
  • We hypothesize that salt efflux, with its accompanying water efflux, results in cell shrinkage that is conducive to glioma cell migration through the minute extracellular spaces of brain. (
  • Gal-3 is located in the nucleus, cytosol, mitochondria, cell surface and extracellular space and is also secreted. (
  • Tendon healing requires migration of tendon cells to the repair site, followed by proliferation and synthesis of the extracellular matrix. (
  • However, individual cells were only polarized on surfaces in contact with the lumen or extracellular matrix. (
  • The extracellular matrix (ECM) provides not only the structural and biochemical support, but also plays a major role in regulating cell behavior. (
  • citation needed] Collective cell migration is enhanced by geometrical confinement of an extracellular matrix molecule (e.g. the proteoglycan versican in neural crest cells), that acts as a barrier, to promote the emergence of organized migration in separated streams. (
  • p90 ribosomal S6 kinase (p90RSK) constitutes a family of serine/threonine kinases that have been shown to be involved in cell proliferation of various malignancies via direct or indirect effects on the cell-cycle machinery. (
  • Additionally, we demonstrated that inhibition of chemotaxis was mediated through a G i /phosphoinositide-3-OH kinase-dependent pathway. (
  • IκB kinase β plays a critical role in TNFR1 phosphorylation of S381, which leads to subsequent T cell migration and accumulation. (
  • Redox cycling by juglone/ascorbate inhibits cell proliferation. (
  • In addition, EGFR-mediated migration was also inhibited by the receptor Kiss1, which not only is structurally unrelated to Eph receptors but also inhibits cell migration by suppressing EGFR recycling. (
  • Objectives- Nitric oxide inhibits smooth muscle cell migration after arterial injury, but the detailed mechanism is not fully understood. (
  • In order to identify novel markers of cells participating to destructive processes in extracapillary glomerular diseases, we carried out comparative analyses of RNA sequencing data from freshly isolated glomeruli from mice with a time course after nephrotoxic serum (NTS)-induced CGN at day 4 and day 10 (Fig. 1a ). (
  • Macrophage migration inhibition studies with cells from mice vaccin- ated with cell walls of mycobacterium bovis bcg. (
  • In xenograft mice models, these expanded NK cells could be efficiently recruited toward CXCL10+ melanomas (53). (
  • Then, the CCA cells that received various treatments were implanted to mice to establish animal model, followed by tumor observation and HE staining to evaluate lung metastasis. (
  • Methods: To test this hypothesis, we cultured primary tracheal epithelial cells in monolayers from both wild type and dimethylarginine dimethylaminohydrolase (DDAH) overexpressing C57Bl/6 mice and measured wound repair using the electric cell impedance sensing (ECIS) system. (
  • Results: Migration of epithelial cell monolayers from wild type mice significantly promoted repair of the ECIS-standardized wound as determined by resistance. (
  • In cells from DDAH overexpressing mice, wounds were repaired up to 8 hr earlier than wild type mice. (
  • Anti-metastatic properties of a potent herbal combination in cell and mice models of triple negative breast cancer. (
  • Neural crest cells in mice, Leghorn chicks, amphibians (Xenopus laevis), and fish (zebrafish): collective migration of neural crest cells occurs during embryo development of vertebrates. (
  • NO production in ECs, which is required for VEGF‐induced Ca 2+ influx and cell migration. (
  • Gal-3 also promotes β-catenin/Wnt signaling and induces cyclin D1 and c-Myc in TSCC and breast cancer cells ( 8 , 9 ). (
  • Actomyosin-mediated contractility in ovarian cancer cells induces mesothelial cells to de-adhere and move away, facilitating penetration of the mesothelial barrier during metastasis. (
  • Complement factor C5a slightly increases the expression of IL-17 and induces activation of Akt in anti-CD3-activated lupus effector T cells. (
  • Carcinoma is a malignant cancer originating in the ectodermal and endodermal epithelial cells. (
  • Thus, upregulation of cell surface HS may play an active and crucial role in directing malignant phenotype of cancer during different developmental stages. (
  • In previous clinical trials, nivolumab has shown activity in several tumor types, including melanoma, renal cell carcinoma, non-small cell lung cancer (NSCLC), hodgkin lymphoma, and malignant pleural mesothelioma. (
  • This public-private collaboration enabled researchers identify molecular mechanisms underlying the lack of efficiency of this drug in squamous cell carcinoma, a sub-type of non-small cell lung cancer, and the important role of smoking in this lack of effectivity. (
  • The primary aim of the current study was to investigate the antitumor effects of ferruginol in OVCAR‑3 human ovary cancer cells. (
  • Treatment of ovarian cancer usually involves surgical resection to reduce the number of cancerous cells, which is followed by adjuvant chemotherapy using taxol/platinum-based drugs. (
  • It has been reported that a large fraction of the ovarian cancer cell population is in a dormant and non-proliferating stage, which results in the failure of the majority of chemotherapeutic agents as cytotoxic chemotherapeutic agents kill fast proliferating cells and spare non-proliferating cells, which subsequently acquire resistance. (
  • Therefore, based on the failure of chemotherapy in these cases, and the high recurrence and acquired drug resistance of ovarian tumors, novel, effective and relatively non-toxic anticancer agents that target ovarian cancer cells are required. (
  • The primary aim of the present study was to determine the antiproliferative effects of ferruginol, a naturally occurring phenolic meroterpene (abietane diterpene), on OVCAR-3 human ovary cancer cells. (
  • The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. (
  • Certain natural compounds from Chinese medicinal herbs have been identified to be cytotoxic to cancer cells whilst remaining non-toxic to normal cells, including taxinol, taxanes, camptothecins, epipodophyllo, curcumin, genistein and perillyl alcohol ( 8 , 9 ). (
  • Further studies have demonstrated that GLPs directly or indirectly mediate cancer cell death through diverse mechanisms ( 14 - 16 ). (
  • Methods: miR-623 was either overexpressed in breast cancer cell lines through exogenous transfection or knocked down by specific siRNA. (
  • We began our experiment by overexpressing miR-623 in breast cancer cell lines MDA-MB-453 and MCF7. (
  • Thus, we believe that the direct influence of NPs on cancer cell metastasis is a promising study topic. (
  • They are the main fuel for cancer cell growth and are used for biosynthesis and energy generation. (
  • While most of the results obtained with docosahexaenoic acid and/or eicosapentaenoic acid show a decrease of tumor cell proliferation and/or aggressivity, there is some evidence that other lipids, which accumulate in breast cancer tissues, such as arachidonic acid may have opposite effects. (
  • Some additional evidence hints that DHA not only acts as an antiproliferative agent by lengthening the cell cycle between the G2/M transition [ 20 ], but is also a proapoptotic factor, increasing Bcl-2, procaspase-8, and caspase-3 activity in breast cancer cell lines [ 21 - 23 ]. (
  • Renal cell carcinoma (RCC) is one of the leading causes of cancer related mortality worldwide. (
  • First, quantitative real-time PCR (qRT-PCR) was performed to detect miR-203 expression in renal cancer cell lines and clear cell RCC (ccRCC) specimens. (
  • Further investigation showed that ectopic expression of FGF2 partially reversed the inhibition effect of enforced miR-203 expression on the malignant phenotypes of renal cancer cells. (
  • Lung cancer can be divided into two broad categories: non-small cell lung cancer (NSCLC), which consists of about 85% of all lung cancers and small cell lung cancer (SCLC), which account for 15% of all lung cancers. (
  • Human colon cancer cell lines HT29-D4 and SW480 were used as cell models. (
  • Therefore, using genetic analysis of border cell migration in Drosophila is a powerful approach to identify novel molecules that promote ovarian cancer dissemination and represent potential therapeutic targets. (
  • Signaling through the epidermal growth factor receptor stimulates ovarian cancer cell migration ( 9 ) and guides migration of border cells ( 10 ). (
  • Small interfering RNA ( siRNA ) was transfected together with LipofectamineTM2000 in T24 human bladder cancer cells to block the YAP signal pathway . (
  • In recurrent and/or metastatic squamous cell carcinoma of the head and neck (R/M-SCCHN), the armamentarium of systemic anti- cancer modalities continues to grow in parallel with innovations in and better integration of local approaches. (
  • Recently, the cell surface protease-activated receptor (PAR)-1 has been shown to act as a chemokine receptor in inflammatory cells, and its expression is tightly correlated with metastatic propensity of breast cancer cells. (
  • We found that the highly invasive MDAMB231 breast cancer cell line expressed very high levels of functional PAR1, PAR2, and PAR4, whereas minimally invasive MCF7 cells had trace amounts of PAR1 and low levels of PAR2 and PAR4. (
  • (13) demonstrated that PAR1 expression levels are directly correlated with degree of invasiveness in both primary breast tissue specimens and established cancer cell lines. (
  • Circular RNAs (circRNAs), a group of non-coding RNAs, play an important role in cancer biology, and in the present study, we aimed to clarify the expression profiles and biological functions of circRNA circVANGL1 in non-small cell lung cancer (NSCLC). (
  • Non-small cell lung cancer (NSCLC), accounting for 85% of all cases, is the most prevalent histological type of lung cancer [ 1 ]. (
  • Dr. Devi's research interests include functional genomics, anti-cancer drug discovery and development, mechanisms of cancer cell signaling, tumor immunity and applications thereof for overcoming therapeutic resistance in cancer. (
  • One of the main reasons patients do not respond to PD-1 blockade is because the T cells never make it into the tumor to attack the cancer cells," said lead author Gabriel Abril-Rodriguez, a doctoral candidate in the departments of pharmacology and medicine in the David Geffen School of Medicine at UCLA. (
  • Currently available therapies are only moderately effective in targeting cancer cells. (
  • Established cancer treatment protocols fail to eliminate populations of cancer stem cells (CSCs), which develop resistance against the chemotherapeutic drugs and lead to cancer recurrence. (
  • In this study, we investigated a series of novel markers to improve the characterization of CSCs, with particular focus the roles of Akt in CSC maintenance and the regulatory role of micro-RNA (miR) in cancer cells. (
  • These findings suggest a significant resting Cl − conductance in glioma cells. (
  • In particular, we confirm the experimental findings that inhibition of homotype gap junctions favours migration while heterotype inhibition hinders it. (
  • These findings suggest that Notch inhibition may prove to be a promising future research direction in the development of clinical interventions for corneal epithelium healing. (
  • Our findings provide a molecular insight on understanding drug resistance in HCC cells. (
  • Our findings demonstrate that targeting CXCR3 is effective in both tumor and host compartments, and suggest that CXCR3 inhibition is likely to avoid adverse effects on host cells. (
  • Expression of PTEN cDNA in glioma cells with mutated PTEN alleles suppresses growth, but it does not affect the growth of cells with wild-type PTEN alleles ( 5 ). (
  • To assess the potential role of these channels in cell migration, we studied the chemotactic migration of glioma cells toward laminin or vitronectin in a Boyden chamber containing transwell filters with 8 μm pores. (
  • We propose that I Cl,Vol contributes to cell shape and volume changes required for glioma cell migration through brain tissue. (
  • Hence, chloride channels may aid the invasive biology of glioma cells, a feature that greatly compromises surgical treatment of this disease ( Adams and Victor, 1989 ). (
  • We present a model for the migration of glioma cells on substrates of collagen and astrocytes. (
  • By the time the tumour is diagnosed, glioma cells have migrated over large distances well beyond the main bulk of the tumour. (
  • Less is known about the role of glioma cell-glioma cell and glioma cell-normal astrocyte communication, and especially the role of gap junctions (GJs). (
  • These studies show that glioma cells can establish functional GJs with both other glioma cells (homotype GJs) and surrounding astrocytes (heterotype GJs). (
  • This conclusion reinforces the idea of cooperation between host astrocytes and glioma cells. (
  • Modelling can help to answer some open questions and leads to formulating hypotheses about the underlying mechanisms of migration of glioma cells. (
  • KU-60019 is 10-fold more effective than KU-55933 at blocking radiation-induced phosphorylation of key ATM targets in human glioma cells. (
  • As expected, KU-60019 is a highly effective radiosensitizer of human glioma cells. (
  • LncRNA MALAT1 has been proved to be involved in the development of various types of human cancers while the involvement of MALAT1 in tongue squamous cell carcinoma has not been reported. (
  • In view of this, our study aimed to investigate the functionality of MALAT1 in tongue squamous cell carcinoma. (
  • MALAT1 may serve as a target for the treatment of tongue squamous cell carcinoma. (
  • After 24 hours, migration was quantified by counting cell numbers at the indicated migration distances from the wound edge. (
  • 2. Inhibition of Notch signaling allows actin cytoskeletal changes that may favor migration, thus providing a possible mechanism for promotion of corneal wound closure. (
  • The monolayer responds with cell spreading and migration into the denuded region until the wound is closed. (
  • In experiments on wounded epithelial monolayers, Farooqui and Fenteany eliminated several biochemical routes for cell-cell communication, including paracrine mechanisms and gap junction signaling, and found that wound healing rates were not significantly affected ( Farooqui and Fenteany, 2005 ). (
  • The authors also discovered that epithelial cells, while maintaining apical contacts with the cells around them, appear to be actively and somewhat independently crawling during wound healing. (
  • With these data, Farooqui and Fenteany raised the possibility that crawling cells, even cells interior to the surviving monolayer, might be guided by a lower mechanical resistance in the direction of the wound. (
  • Previously, we have reported that bronchial epithelial cell exposure to swine CAFO dust significantly blunts normative cell migration and wound repair via a PKC-dependent mechanism. (
  • Importantly, a significant enhancement of wound repair (25% increase in resistance) was observed in DDAH cells vs. wild type cells treated with swine CAFO dust for 24 hr. (
  • Wound healing: collective cell migration is an essential part in this healing process, wound area is closed by the migrating cells. (
  • The results demonstrated that the GLP‑mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. (
  • The microtubule-binding natural products paclitaxel, vinblastine, colchicine and podophyllotoxin significantly altered microtubule dynamics in living cells and inhibited cell migration at concentrations below apparent cytotoxicity. (
  • In this study we show that small molecule inhibitors and gene silencing of MRP1 had a significant effect on GBM cell response to temozolomide (150 μM), vincristine (100 nM), and etoposide (2 μM). (
  • Firstly, we profiled its gene expression pattern by microarray analysis of HOTAIR loss in Bel-7402 HCC cell line. (
  • Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. (
  • In contrast, the expression of antisense PTEN enhanced cell migration (Fig. 1 ). (
  • With regard to cell growth, in contrast, expression of sense or antisense PTEN had little effect ( 8 ), consistent with a previous report ( 5 ). (
  • Stable NIH 3T3 cell transfectants were induced to express PTEN or antisense PTEN in an ecdysone expression system ( 7 ). (
  • In the cell models expressing mutant SERCA, biotinylated-iodoacetamide (BIAM) and biotinylated-glutathione labeling of SERCA was decreased, and NO failed to increase SERCA activity or decrease Ca 2+ influx, thus validating that the expression of mutant SERCA prevents its redox-dependent activation. (
  • NFkB inhibition after corneal penetrating injury suppresses the expression of MIP1a in corneal epithelium, and prevents the migration of inflammatory cells in corneal stroma and anterior chamber. (
  • In addition, the expression of miR-625-3p and its targets was detected in five human CRC cell lines. (
  • Mechanistically, CD9 deficiency prevents the oriented migration of PECs into the glomerular tuft and their acquisition of CD44 and β1 integrin expression. (
  • We further show that high CD9 expression is required for full activation of the HB-EGF-EGFR and PDGF-BB-PDGFR pathways and thus, CD9 lowers threshold for oriented PEC migration. (
  • Western blot and RT-PCR were performed to investigate the expression of FASN in U2-OS cells. (
  • The real-time RT-PCR was used to measure the miR-130a-3p expression in GR HCC cells compared with their parental cells. (
  • Western blotting analysis was used to measure the expression of Smad4, E-cadherin, Vimentin, and MMP-2 in GR HCC cells after depletion of Smad4. (
  • The underlying mechanisms involve a decreased expression of IL-4, IL-10, iNOs, and Arg-1 in myeloid cells and an increased T cell response. (
  • Here, we demonstrate that knock-down of the endogenous Thy-1 expression by Thy-1 siRNA transfection promoted the migration of human umbilical vein endothelial cells (HUVEC). (
  • We recently showed that CXCR2 expression is downregulated on tumor-infiltrating NK cells in RCC and genetic modification to re-express CXCR2 enhanced recruitment of NK cells to the tumor site (39). (
  • Many factors can modify CXCR3 expression about T NK and cells cells. (
  • Additionally, STAT3 diminished CXCR3 expression on CD8+ T cells (57). (
  • To enhance effector cell recruitment, efforts are made to increase CXCL9 and CXCL10 expression within the TME. (
  • CCA tissues and cells were observed to have a high expression of NUAK1 and poor expression of miR-1182 and let-7a. (
  • Despite the differences in expression, PAR2 and PAR4 acted as chemokine receptors in both invasive and minimally invasive breast cell lines. (
  • In addition, we predicted that circVANGL1 might serve as a competing endogenous RNA (ceRNA), becoming a sink for miR-195, thereby modulating the expression of Bcl-2 in NSCLC cells. (
  • Whether a correlation exits between this effect and the expression of paxillin, which is a positive regulator of cell spreading and migration, was also investigated. (
  • Gab1 expression was up-regulated in response to SDF-1 stimulation in CS cell line JJ012, SW1353, L3252. (
  • CDK-dependent pRb hyperphosphorylation releases E2F transcription factors, thus contributing to the expression of several growth and cell cycle-regulatory genes with functional E2F-binding sites in their promoters. (
  • Integrin-mediated cell spreading and the formation of focal adhesions were down-regulated by wild-type PTEN but not by PTEN with an inactive phosphatase domain. (
  • RhoA/ROCK inhibition reduces focal adhesions and traction forces, while promoting a novel gliding mode of migration. (
  • Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. (
  • Therefore, in the current study, we explore the role and molecular mechanisms of miR-130a-3p in gemcitabine resistant (GR) hepatocellular carcinoma (HCC) cells. (
  • 1 Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Str.11, 44227 Dortmund, Germany. (
  • 1,2 Thus, understanding the molecular mechanisms that control hyperplastic growth and the locomotion of vascular cells should aid in the development of novel therapeutic strategies to reduce neointimal thickening. (
  • Here, we found that E 2 treatment decreased cell proliferation and cell cycle-regulating factors such as cyclin A, cyclin D1 and cyclin E. At the same time, E 2 significantly inhibited cell migration and migration-related factors such as uPA, tPA, MMP-2, and MMP-9. (
  • Indeed, we have determined that azathioprine treatment of cultured human PDAC cells reduces viability and proliferation. (
  • Mammalian target of rapamycin inhibition by rapamycin reduces both Stat3 activation in effector T cells and the frequency of IL-17-producing T cells in lupus patients. (
  • and biochemical processes governing the active motion of the cell (for a review, see Demuth & Berens (2004) ). (
  • Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. (
  • Both processes required the transportation of lipid rafts to the cell surface to deliver signaling components. (
  • Over these serial processes, the cell undergoes multiple attachments and detachments to endothelial cells ( 1 ). (
  • Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho GTPases, Rac1 and Cdc42. (
  • If the conversion of pyruvate to lactate is reduced, cells excessively use the oxidative phosphorylation flux as a metabolic pathway to generate ATP, resulting in ROS due to oxidative stress, eventually leading to cell death 14 . (
  • The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. (
  • Materials and Methods : Human osteosarcoma cell lines U2-OS and osteosarcoma biopsy specimens were employed in this study. (
  • in fact, it had been significantly improved, as was success of cultured human being saphenous vein endothelial cells. (
  • Methods Cell tradition Surplus sections of human being saphenous vein had been obtained from individuals going through coronary artery bypass medical procedures (Study Ethical Committee quantity 04/Q2007/6). (
  • The results showed that circVANGL1 was overexpressed in human NSCLC tissues and cell lines. (
  • Application of RANTES, MIP-1α, MIP-1β, or MCP-1 chemokines that are secreted by leukocytes (16) stimulates chemotaxis of MCF-7 cells. (
  • In control group, marked migration of neutrophils was detected in anterior chamber from 6 to 12hours after corneal penetrating injury, and macrophages were detected in corneal stroma at 24hour. (
  • In another scholarly study, STAT3 signaling SGX-523 price in Compact disc8+ T cells was proven to downregulate IFN creation, leading to reduced CXCL10 manifestation by tumor-associated macrophages. (
  • Splenic cells become sensitive 2 to 3 weeks after immunization and hepatic macrophages become sensitive only several weeks later. (