Cell Migration Assays
Cell Migration Assays, Macrophage
Cell Migration Assays, Leukocyte
Cell Migration Inhibition
Macrophage Migration-Inhibitory Factors
RNA, Small Interfering
Macrophage Colony-Stimulating Factor
Reverse Transcriptase Polymerase Chain Reaction
rac1 GTP-Binding Protein
Matrix Metalloproteinase 9
Gene Expression Regulation
Gene Knockdown Techniques
Focal Adhesion Protein-Tyrosine Kinases
Gene Expression Regulation, Neoplastic
Tumor Cells, Cultured
Dose-Response Relationship, Drug
Tumor Necrosis Factor-alpha
Cell Adhesion Molecules
Molecular Sequence Data
Matrix Metalloproteinase 2
Disease Models, Animal
rac GTP-Binding Proteins
Diffusion Chambers, Culture
Culture Media, Conditioned
Cell Surface Extensions
Bone Marrow Cells
Vascular Endothelial Growth Factor A
Proto-Oncogene Proteins c-akt
Platelet-Derived Growth Factor
Myocytes, Smooth Muscle
Macrophage Inflammatory Proteins
rho GTP-Binding Proteins
Receptors, Cell Surface
Real-Time Polymerase Chain Reaction
rhoA GTP-Binding Protein
cdc42 GTP-Binding Protein
Amino Acid Sequence
Focal Adhesion Kinase 1
Enzyme-Linked Immunosorbent Assay
Antigens, Differentiation, Myelomonocytic
Intercellular Signaling Peptides and Proteins
Transendothelial and Transepithelial Migration
Epidermal Growth Factor
Mitogen-Activated Protein Kinase 3
Receptor, Macrophage Colony-Stimulating Factor
Extracellular Signal-Regulated MAP Kinases
MAP Kinase Signaling System
Transforming Growth Factor beta
NIH 3T3 Cells
Fluorescent Antibody Technique
Gene Expression Profiling
Fibroblast Growth Factor 2
Proto-Oncogene Proteins c-met
Crk-Associated Substrate Protein
Nitric Oxide Synthase Type II
Mesenchymal Stromal Cells
Gene Expression Regulation, Developmental
Protein Structure, Tertiary
Scavenger Receptors, Class A
Adaptor Proteins, Signal Transducing
Mice, Inbred Strains
Biological effects of a sulfated-polysaccharide isolated from the marine red algae Champia feldmannii. (1/7)Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested i.v. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9 mg/kg (1.01+/-0.030 x 0.06+/-0.03 ml) compared to controls (0.06+/-0.03 ml), increased vascular-permeability (38.44+/-12.63 x 11.29+/-3.91 microg/g) and stimulated neutrophil migration (3.348+/-295 x 307+/-99 cells/microl) 1 h after injection. Cf-PLS was also antinociceptive (6.6+/-1.28 x 33+/-1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant. (+info)
Matrix metalloproteinase-9 is associated with acute inflammation after olfactory injury. (2/7)(+info)
Macrophage infiltration predicts a poor prognosis for human ewing sarcoma. (3/7)(+info)
Nox2 is required for macrophage chemotaxis towards CSF-1. (4/7)(+info)
Heparanase induced by advanced glycation end products (AGEs) promotes macrophage migration involving RAGE and PI3K/AKT pathway. (5/7)(+info)
CD14 directs adventitial macrophage precursor recruitment: role in early abdominal aortic aneurysm formation. (6/7)(+info)
In vivo fluorescence-mediated tomography imaging demonstrates atorvastatin-mediated reduction of lesion macrophages in ApoE-/- mice. (7/7)(+info)
1. Tumor size and location: Larger tumors that have spread to nearby tissues or organs are generally considered more invasive than smaller tumors that are confined to the original site.
2. Cellular growth patterns: The way in which cancer cells grow and divide can also contribute to the overall invasiveness of a neoplasm. For example, cells that grow in a disorganized or chaotic manner may be more likely to invade surrounding tissues.
3. Mitotic index: The mitotic index is a measure of how quickly the cancer cells are dividing. A higher mitotic index is generally associated with more aggressive and invasive cancers.
4. Necrosis: Necrosis, or the death of cells, can be an indication of the level of invasiveness of a neoplasm. The presence of significant necrosis in a tumor is often a sign that the cancer has invaded surrounding tissues and organs.
5. Lymphovascular invasion: Cancer cells that have invaded lymphatic vessels or blood vessels are considered more invasive than those that have not.
6. Perineural invasion: Cancer cells that have invaded nerve fibers are also considered more invasive.
7. Histological grade: The histological grade of a neoplasm is a measure of how abnormal the cancer cells look under a microscope. Higher-grade cancers are generally considered more aggressive and invasive than lower-grade cancers.
8. Immunohistochemical markers: Certain immunohistochemical markers, such as Ki-67, can be used to evaluate the proliferative activity of cancer cells. Higher levels of these markers are generally associated with more aggressive and invasive cancers.
Overall, the degree of neoplasm invasiveness is an important factor in determining the likelihood of the cancer spreading to other parts of the body (metastasizing) and in determining the appropriate treatment strategy for the patient.
Foreign-body migration refers to the movement or migration of a foreign object or material within the body over time. This can occur after a surgical procedure, injury, or other medical intervention where a foreign object is introduced into the body. The term "foreign body" includes any object or material that is not naturally present within the body, such as implants, sutures, staples, and other medical devices.
The migration of a foreign body can occur due to various factors, including:
1. Mechanical forces: Movement of the body, such as during exercise or daily activities, can cause the foreign object to shift position or migrate to another part of the body.
2. Biological forces: The body's natural healing processes and inflammatory responses can cause the foreign object to move or change shape over time.
3. Chemical forces: Corrosion or degradation of the foreign material can lead to its migration within the body.
4. Cellular forces: Cells in the body can surround and interact with the foreign object, leading to its movement or displacement.
The migration of a foreign body can have significant clinical implications, including:
1. Pain and discomfort: The movement of a foreign object within the body can cause pain, discomfort, and inflammation.
2. Infection: The migration of a foreign object can increase the risk of infection, particularly if the object is made of a material that is susceptible to bacterial growth.
3. Organ damage: If the migrated foreign object damages surrounding tissues or organs, it can lead to serious complications and long-term health problems.
4. Revision surgery: In some cases, the migration of a foreign body may require revision surgery to remove or reposition the object.
To prevent foreign-body migration, medical professionals use various techniques, such as:
1. Implant fixation: Implants can be fixed in place using bone screws, sutures, or other fixation devices to minimize their movement.
2. Biocompatible materials: Using biocompatible materials for implants and other medical devices can reduce the risk of foreign-body reaction and migration.
3. Proper surgical technique: Surgeons must use proper surgical techniques when inserting foreign objects into the body, such as using a sterile environment and appropriate insertion angles.
4. Postoperative care: Proper postoperative care, including antibiotics and pain management, can help prevent complications and promote healing.
Overall, preventing the migration of foreign bodies is essential to ensure successful medical outcomes and minimize the risk of complications.
Neoplastic metastasis can occur in any type of cancer but are more common in solid tumors such as carcinomas (breast, lung, colon). It is important for cancer diagnosis and prognosis because metastasis indicates that the cancer has spread beyond its original site and may be more difficult to treat.
Metastases can appear at any distant location but commonly found sites include the liver, lungs, bones, brain, and lymph nodes. The presence of metastases indicates a higher stage of cancer which is associated with lower survival rates compared to localized cancer.
Pathologic neovascularization can be seen in a variety of conditions, including cancer, diabetic retinopathy, and age-related macular degeneration. In cancer, for example, the formation of new blood vessels can help the tumor grow and spread to other parts of the body. In diabetic retinopathy, the growth of new blood vessels in the retina can cause vision loss and other complications.
There are several different types of pathologic neovascularization, including:
* Angiosarcoma: a type of cancer that arises from the cells lining blood vessels
* Hemangiomas: benign tumors that are composed of blood vessels
* Cavernous malformations: abnormal collections of blood vessels in the brain or other parts of the body
* Pyogenic granulomas: inflammatory lesions that can form in response to trauma or infection.
The diagnosis of pathologic neovascularization is typically made through a combination of physical examination, imaging studies (such as ultrasound, CT scans, or MRI), and biopsy. Treatment options vary depending on the underlying cause of the condition, but may include medications, surgery, or radiation therapy.
In summary, pathologic neovascularization is a process that occurs in response to injury or disease, and it can lead to serious complications. It is important for healthcare professionals to be aware of this condition and its various forms in order to provide appropriate diagnosis and treatment.
1) They share similarities with humans: Many animal species share similar biological and physiological characteristics with humans, making them useful for studying human diseases. For example, mice and rats are often used to study diseases such as diabetes, heart disease, and cancer because they have similar metabolic and cardiovascular systems to humans.
2) They can be genetically manipulated: Animal disease models can be genetically engineered to develop specific diseases or to model human genetic disorders. This allows researchers to study the progression of the disease and test potential treatments in a controlled environment.
3) They can be used to test drugs and therapies: Before new drugs or therapies are tested in humans, they are often first tested in animal models of disease. This allows researchers to assess the safety and efficacy of the treatment before moving on to human clinical trials.
4) They can provide insights into disease mechanisms: Studying disease models in animals can provide valuable insights into the underlying mechanisms of a particular disease. This information can then be used to develop new treatments or improve existing ones.
5) Reduces the need for human testing: Using animal disease models reduces the need for human testing, which can be time-consuming, expensive, and ethically challenging. However, it is important to note that animal models are not perfect substitutes for human subjects, and results obtained from animal studies may not always translate to humans.
6) They can be used to study infectious diseases: Animal disease models can be used to study infectious diseases such as HIV, TB, and malaria. These models allow researchers to understand how the disease is transmitted, how it progresses, and how it responds to treatment.
7) They can be used to study complex diseases: Animal disease models can be used to study complex diseases such as cancer, diabetes, and heart disease. These models allow researchers to understand the underlying mechanisms of the disease and test potential treatments.
8) They are cost-effective: Animal disease models are often less expensive than human clinical trials, making them a cost-effective way to conduct research.
9) They can be used to study drug delivery: Animal disease models can be used to study drug delivery and pharmacokinetics, which is important for developing new drugs and drug delivery systems.
10) They can be used to study aging: Animal disease models can be used to study the aging process and age-related diseases such as Alzheimer's and Parkinson's. This allows researchers to understand how aging contributes to disease and develop potential treatments.
There are several key features of inflammation:
1. Increased blood flow: Blood vessels in the affected area dilate, allowing more blood to flow into the tissue and bringing with it immune cells, nutrients, and other signaling molecules.
2. Leukocyte migration: White blood cells, such as neutrophils and monocytes, migrate towards the site of inflammation in response to chemical signals.
3. Release of mediators: Inflammatory mediators, such as cytokines and chemokines, are released by immune cells and other cells in the affected tissue. These molecules help to coordinate the immune response and attract more immune cells to the site of inflammation.
4. Activation of immune cells: Immune cells, such as macrophages and T cells, become activated and start to phagocytose (engulf) pathogens or damaged tissue.
5. Increased heat production: Inflammation can cause an increase in metabolic activity in the affected tissue, leading to increased heat production.
6. Redness and swelling: Increased blood flow and leakiness of blood vessels can cause redness and swelling in the affected area.
7. Pain: Inflammation can cause pain through the activation of nociceptors (pain-sensing neurons) and the release of pro-inflammatory mediators.
Inflammation can be acute or chronic. Acute inflammation is a short-term response to injury or infection, which helps to resolve the issue quickly. Chronic inflammation is a long-term response that can cause ongoing damage and diseases such as arthritis, asthma, and cancer.
There are several types of inflammation, including:
1. Acute inflammation: A short-term response to injury or infection.
2. Chronic inflammation: A long-term response that can cause ongoing damage and diseases.
3. Autoimmune inflammation: An inappropriate immune response against the body's own tissues.
4. Allergic inflammation: An immune response to a harmless substance, such as pollen or dust mites.
5. Parasitic inflammation: An immune response to parasites, such as worms or fungi.
6. Bacterial inflammation: An immune response to bacteria.
7. Viral inflammation: An immune response to viruses.
8. Fungal inflammation: An immune response to fungi.
There are several ways to reduce inflammation, including:
1. Medications such as nonsteroidal anti-inflammatory drugs (NSAIDs), corticosteroids, and disease-modifying anti-rheumatic drugs (DMARDs).
2. Lifestyle changes, such as a healthy diet, regular exercise, stress management, and getting enough sleep.
3. Alternative therapies, such as acupuncture, herbal supplements, and mind-body practices.
4. Addressing underlying conditions, such as hormonal imbalances, gut health issues, and chronic infections.
5. Using anti-inflammatory compounds found in certain foods, such as omega-3 fatty acids, turmeric, and ginger.
It's important to note that chronic inflammation can lead to a range of health problems, including:
3. Heart disease
5. Alzheimer's disease
6. Parkinson's disease
7. Autoimmune disorders, such as lupus and rheumatoid arthritis.
Therefore, it's important to manage inflammation effectively to prevent these complications and improve overall health and well-being.
There are several types of gliomas, including:
1. Astrocytoma: This is the most common type of glioma, accounting for about 50% of all cases. It arises from the star-shaped cells called astrocytes that provide support and nutrients to the brain's nerve cells.
2. Oligodendroglioma: This type of glioma originates from the oligodendrocytes, which are responsible for producing the fatty substance called myelin that insulates the nerve fibers.
3. Glioblastoma (GBM): This is the most aggressive and malignant type of glioma, accounting for about 70% of all cases. It is fast-growing and often spreads to other parts of the brain.
4. Brain stem glioma: This type of glioma arises in the brain stem, which is responsible for controlling many of the body's vital functions such as breathing, heart rate, and blood pressure.
The symptoms of glioma depend on the location and size of the tumor. Common symptoms include headaches, seizures, weakness or numbness in the arms or legs, and changes in personality, memory, or speech.
Gliomas are diagnosed through a combination of imaging tests such as CT or MRI scans, and tissue biopsy to confirm the presence of cancer cells. Treatment options for glioma depend on the type and location of the tumor, as well as the patient's overall health. Surgery is often the first line of treatment to remove as much of the tumor as possible, followed by radiation therapy and/or chemotherapy to kill any remaining cancer cells.
The prognosis for glioma patients varies depending on the type and location of the tumor, as well as the patient's overall health. In general, the prognosis is better for patients with slow-growing, low-grade tumors, while those with fast-growing, high-grade tumors have a poorer prognosis. Overall, the 5-year survival rate for glioma patients is around 30-40%.
There are several types of melanoma, including:
1. Superficial spreading melanoma: This is the most common type of melanoma, accounting for about 70% of cases. It usually appears as a flat or slightly raised discolored patch on the skin.
2. Nodular melanoma: This type of melanoma is more aggressive and accounts for about 15% of cases. It typically appears as a raised bump on the skin, often with a darker color.
3. Acral lentiginous melanoma: This type of melanoma affects the palms of the hands, soles of the feet, or nail beds and accounts for about 5% of cases.
4. Lentigo maligna melanoma: This type of melanoma usually affects the face and is more common in older adults.
The risk factors for developing melanoma include:
1. Ultraviolet (UV) radiation exposure from the sun or tanning beds
2. Fair skin, light hair, and light eyes
3. A history of sunburns
4. Weakened immune system
5. Family history of melanoma
The symptoms of melanoma can vary depending on the type and location of the cancer. Common symptoms include:
1. Changes in the size, shape, or color of a mole
2. A new mole or growth on the skin
3. A spot or sore that bleeds or crusts over
4. Itching or pain on the skin
5. Redness or swelling around a mole
If melanoma is suspected, a biopsy will be performed to confirm the diagnosis. Treatment options for melanoma depend on the stage and location of the cancer and may include surgery, chemotherapy, radiation therapy, or a combination of these. Early detection and treatment are key to successful outcomes in melanoma cases.
In conclusion, melanoma is a type of skin cancer that can be deadly if not detected early. It is important to practice sun safety, perform regular self-exams, and seek medical attention if any suspicious changes are noticed on the skin. By being aware of the risk factors, symptoms, and treatment options for melanoma, individuals can take steps to protect themselves from this potentially deadly disease.
There are different types of Breast Neoplasms such as:
1. Fibroadenomas: These are benign tumors that are made up of glandular and fibrous tissues. They are usually small and round, with a smooth surface, and can be moved easily under the skin.
2. Cysts: These are fluid-filled sacs that can develop in both breast tissue and milk ducts. They are usually benign and can disappear on their own or be drained surgically.
3. Ductal Carcinoma In Situ (DCIS): This is a precancerous condition where abnormal cells grow inside the milk ducts. If left untreated, it can progress to invasive breast cancer.
4. Invasive Ductal Carcinoma (IDC): This is the most common type of breast cancer and starts in the milk ducts but grows out of them and invades surrounding tissue.
5. Invasive Lobular Carcinoma (ILC): It originates in the milk-producing glands (lobules) and grows out of them, invading nearby tissue.
Breast Neoplasms can cause various symptoms such as a lump or thickening in the breast or underarm area, skin changes like redness or dimpling, change in size or shape of one or both breasts, discharge from the nipple, and changes in the texture or color of the skin.
Treatment options for Breast Neoplasms may include surgery such as lumpectomy, mastectomy, or breast-conserving surgery, radiation therapy which uses high-energy beams to kill cancer cells, chemotherapy using drugs to kill cancer cells, targeted therapy which uses drugs or other substances to identify and attack cancer cells while minimizing harm to normal cells, hormone therapy, immunotherapy, and clinical trials.
It is important to note that not all Breast Neoplasms are cancerous; some are benign (non-cancerous) tumors that do not spread or grow.
The disease begins with endothelial dysfunction, which allows lipid accumulation in the artery wall. Macrophages take up oxidized lipids and become foam cells, which die and release their contents, including inflammatory cytokines, leading to further inflammation and recruitment of more immune cells.
The atherosclerotic plaque can rupture or ulcerate, leading to the formation of a thrombus that can occlude the blood vessel, causing ischemia or infarction of downstream tissues. This can lead to various cardiovascular diseases such as myocardial infarction (heart attack), stroke, and peripheral artery disease.
Atherosclerosis is a multifactorial disease that is influenced by genetic and environmental factors such as smoking, hypertension, diabetes, high cholesterol levels, and obesity. It is diagnosed by imaging techniques such as angiography, ultrasound, or computed tomography (CT) scans.
Treatment options for atherosclerosis include lifestyle modifications such as smoking cessation, dietary changes, and exercise, as well as medications such as statins, beta blockers, and angiotensin-converting enzyme (ACE) inhibitors. In severe cases, surgical interventions such as bypass surgery or angioplasty may be necessary.
In conclusion, atherosclerosis is a complex and multifactorial disease that affects the arteries and can lead to various cardiovascular diseases. Early detection and treatment can help prevent or slow down its progression, reducing the risk of complications and improving patient outcomes.
Eugene C. Butcher
Cancer stem cell
S. Murty Srinivasula
Clostridium difficile toxin A
Protease-activated receptor 2
Nerve guidance conduit
Specialized pro-resolving mediators
Hematopoietic stem cell
Proteases in angiogenesis
Broad-spectrum chemokine inhibitor
Lipid-loaded tumor-associated macrophages sustain tumor growth and invasiveness in prostate cancer - PubMed
Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis...
MicroRNA-Mediated Calcineurin Signaling Activation Induces CCL2, CCL3, CCL5, IL8 and Chemotactic Activities in 4,4'-Methylene...
ADIPOQ adiponectin, C1Q and collagen domain containing [Homo sapiens (human)] - Gene - NCBI
NIOSHTIC-2 Search Results - Full View
Inhibitory effects of miR‑25 targeting HMGB1 on macrophage secretion of inflammatory cytokines in sepsis
Pesquisa | Prevenção e Controle de Câncer
Pharos : Target Details - PLXNB2
Pathobiology of the 129:Stat1 -/- mouse model of human age-related ER-positive breast cancer with an immune infiltrate-excluded...
Migration Assay Bioteck - PeanutDB Plant Genomic sequence Database
Ldh Cytoselecttm Cell Biolabs Milano Italia - Bionotatki - Biologiczny Portal Informacyjno-Edukacyjny
JCI Insight - β1 Integrin regulates adult lung alveolar epithelial cell inflammation
Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer<...
Anti inflammatory / فول الصويا
CYCLOSPORINE CAPSULES, USP 25 mg and 100 mg Rx only Prescribing Information
Immuno | Free Full-Text | Effect of Wild Blueberry Metabolites on Biomarkers of Gastrointestinal and Immune Health In Vitro
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
Anti-SAA Mouse Monoclonal Antibody [clone: 585] | VWR
Casticin inhibits interleukin-1β-induced ICAM-1 and MUC5AC expression by blocking NF-κB, PI3K-Akt, and MAPK signaling in human...
Primary Macrophage Chemotaxis Induced by Cannabinoid Receptor 2 Agonists Occurs Independently of the CB2 Receptor. - Oxford...
Broad-spectrum CC-chemokine blockade by gene transfer inhibits macrophage recruitment and atherosclerotic plaque formation in...
The αvβ3 integrin as a therapeutic target for inhibition of bone resorption | Arthritis Research & Therapy | Full Text
IL-6 in human cytomegalovirus secretome promotes angiogenesis and survival of endothelial cells through the stimulation of...
- The innate immune signaling molecule CD14 was reported to be upregulated in adventitial macrophages in a murine model of AAA and in monocytes cocultured with aortic adventitial fibroblasts (AoAf) in vitro, concurrent with increased interleukin-6 (IL-6) expression. (nih.gov)
- Adventitial monocyte binding to AngII-infused aorta in vitro was dependent on CD14, and incubation of human acute monocytic leukemia cell line-1 (THP-1) monocytes with IL-6 or conditioned medium from perivascular adipose tissue (PVAT) upregulated CD14 expression. (nih.gov)
- Further, using in vitro model, C2C12, a myoblast cell line, we demonstrated that satellite cells express CCR2 and CCRS receptors and these chemokine pathways are involved in satellite cell migration activity. (cdc.gov)
- Macrophages were cultured in vitro and divided into 5 groups following treatment with lipopolysaccharide (LPS). (spandidos-publications.com)
- 48. In vitro demonstration of cell-mediated immunity against human renal carcinoma assessed by the leukocyte migration assay. (nih.gov)
- Down-regulates macrophage migration in wound-healing assays (in vitro) (By similarity). (nih.gov)
- In vitro cellular assays were carried out in a panel of gallbladder cancer cell lines using MIF inhibitors, ISO-1 and 4-IPP or its specific siRNA. (elsevier.com)
- By an in vitro chemotaxis assay, plasma and aortic homogenates from 35K gene transfer mice promoted significantly less CC-CK-induced cell migration than did PBS or AdGFP controls. (ox.ac.uk)
- In order to examine the effects of signaling between tumor cells and macrophages in intravasation, we utilize an in vitro transendothelial migration (iTEM) assay. (yu.edu)
- 1.63 m in diameter) were used as the date, single cells have been imaged in vitro in culture superparamagnetic label. (nih.gov)
- This method was recently extended to that these particles are efficiently endocytosed by a wide visualizing single cells and single particles in in vitro variety of cell types, with labeling capacity as high as embryo samples by MRI (7). (nih.gov)
- Macrophage migration inhibitory factor (MIF), a multipotent protein that exhibits both cytokine and chemotactic properties, is expressed by many cell types, including hepatocytes and nonparenchymal cells. (nih.gov)
- The expression of macrophage migration inhibitory factor was analysed in gallbladder adenocarcinoma tissues using immunohistochemistry. (elsevier.com)
- Among these, macrophage migration inhibitory factor (MIF) was observed to be highly overexpressed in two of the invasive cell lines. (elsevier.com)
- S protein exposure combined to hypoxia enhanced the production of pro-inflammatory cytokines involved in immune cell activation and trafficking, namely macrophage migration inhibitory factor (MIF). (bvsalud.org)
- Dendritic cell differentiation was prepared using undifferentiated THP-1 monocytes cultured in serum-free RPMI-1640 culture medium supplemented with 100 ng/ml rhGM-CSF, 10 ng/ml rhTNF-α, and 200 ng/ml ionomycin for 3 days. (cdc.gov)
- The 5 µm pore size is ideal for monocytes / macrophages. (bioinfolab.org)
- Upon encountering outside stimuli, alveolar macrophages react by phagocytosis as well as producing and secreting different mediators such as cytokines, chemokines, and others, into the alveoli microenvironment to orchestrate the initiation of inflammatory/immune responses. (cdc.gov)
- Dysfunction of alveolar macrophages, including elevated production and secretion of pro-inflammatory cytokines and other immune mediators, has been shown to play an important role in asthma pathogenesis. (cdc.gov)
- However, both the levels of these asthma-associated, macrophage-secreted inflammatory/immune mediators in MDI-OA patients' airways and how expression of these mediators change in response to MDI exposure in alveolar macrophages are largely undetermined. (cdc.gov)
- Gene expression profiling of myeloid cells in inflamed and malignant colon tissues showed increased expression levels of inflammatory macrophage-associated cytokines compared with normal tissues. (aacrjournals.org)
- The C-C motif chemokine ligand 2 (CCL2) is an inflammatory chemokine that is associated with the migration of macrophages and MSCs during inflammation . (bvsalud.org)
- Acute CCL2 stimulation potentially facilitates osteogenesis during the acute inflammatory phase of bone healing by directing local macrophage migration, fostering macrophage -MSC crosstalk, and subsequently, by activating or licensing of MSCs by macrophage pro-inflammatory cytokines . (bvsalud.org)
- We hypothesize that early inflammatory mediators, such as chemokines, are released during the muscle disruption and degeneration and are involved in macrophage recruitment and satellite cell migration and activation. (cdc.gov)
- Down-regulation of MCP-l and MIP-l J3 responses by application of CCR5 deficient mice and MCP-l neutralizing antibody, resulted in minimal effects in inflammatory cell influx but a significant delay in muscle function recovery. (cdc.gov)
- High mobility group box 1 (HMGB1) can promote the migration of macrophages and the release of inflammatory cytokines, functions associated with the occurrence of sepsis. (spandidos-publications.com)
- The role of microRNA (miR)‑25 in the targeted regulation of HMGB1 expression and the release of macrophage inflammatory cytokines remains uncharacterized. (spandidos-publications.com)
- The present study also investigated whether miR‑25 serves a role in targeting the regulation of HMGB1 expression and macrophage inflammatory factor release. (spandidos-publications.com)
- HMGB1 serves a key role in the occurrence and progression of sepsis, and its production is induced by secretions of immune cells, including mononuclear cells, dendritic cells, macrophages stimulated by endotoxins, and inflammatory cytokines ( 2 ). (spandidos-publications.com)
- A previous study demonstrated that HMGB1 could promote the migration of macrophages and the release of various inflammatory cytokines, causing aggregation of a variety of immune cells and inducing the inflammatory responses of sepsis ( 3 ). (spandidos-publications.com)
- miRs are highly conserved, endogenous, non-coding small RNAs, which can regulate the expression of target genes by complete or incomplete complementary pairing with the 3′-untranslated region (3′-UTR) of the mRNA, serving an important role in immune cell activation, inflammatory cytokine release and the immune response ( 6 , 7 ). (spandidos-publications.com)
- Consequently, macrophages and lymphocytes infiltrate into the adipose tissue and elevate pro-inflammatory cytokine production through TLR activation. (herbal-organic.com)
- were investigated for anti-inflammatory effects, using COX-2 producing PGE(2) inhibitory assay. (herbal-organic.com)
- leaf extract possesses anti-oxidant properties, decreases inflammatory mediator production in murine macrophages, and inhibits growth, migration, and adhesion in human cancer cells. (herbal-organic.com)
- The compound casticin, isolated from Vitex rotundifolia , exerts anti-inflammatory effects and causes apoptosis of cancer cells. (oncotarget.com)
- In this study, we explored the anti-inflammatory effects of casticin and modulation of cyclooxygenase (COX)-2, intercellular adhesion molecule 1 (ICAM-1), and mucin 5AC (MUC5AC) expression in interleukin-1β (IL-1β)-activated A549 human pulmonary epithelial cells. (oncotarget.com)
- A549 cells were treated with various concentrations of casticin (5-20 μM), and an inflammatory response was triggered with interleukin (IL)-1β cytokines. (oncotarget.com)
- Co-culture of NF-κB, MAPK, and PI3K inhibitors with casticin also led to more significantly suppressed ICAM-1 expression in inflammatory A549 cells. (oncotarget.com)
- These results provide evidence that casticin has an anti-inflammatory effect by blocking proinflammatory cytokine, chemokine, and ICAM-1 expression via suppression of the PI3K/Akt, NF-κB, and MAPK signaling pathways in IL-1β-stimulated inflammatory pulmonary epithelial cells. (oncotarget.com)
- With a bacterial or viral infection, activation of macrophages and T cells of airways occurs, and more interleukin (IL)-1β can be detected in inflammatory diseases of the airways [ 5 ]. (oncotarget.com)
- Our histopathological diagnoses were based on inflammatory cell infiltrate patterns and the presence of granulomas and amastigotes. (cdc.gov)
- We analyzed the involvement of macrophage-associated cytokines in the induction of aberrant MUC1 glycoforms. (aacrjournals.org)
- Several cytokines were significantly induced in the HCMV secretomes including interleukin-6 (IL-6), granulocyte macrophage colony-stimulating factor, and IL-8/CXCL8. (elsevierpure.com)
- 42. Indirect macrophage migration inhibition response to 3-M KCl extract of gastric carcinoma. (nih.gov)
- 55. [Macrophage electrophoretic mobility inhibition test (MET) in immunological diagnosis of malignant lymphomas in Papio baboons]. (nih.gov)
- In conditioned media experiments, cells derived from 129:Stat1 -/- tumors secrete both chemoattractant and chemoinhibitory factors, with greater attraction in the extracellular vesicular fraction and inhibition in the soluble fraction. (escholarship.org)
- Experimental evidence suggests that the effectiveness of cyclosporine is due to specific and reversible inhibition of immunocompetent lymphocytes in the G 0 - or G 1 -phase of the cell cycle. (nih.gov)
- Silencing/inhibition of MIF using siRNA and/or MIF antagonists resulted in a significant decrease in cell viability, colony forming ability and invasive property of the gallbladder cancer cells. (elsevier.com)
- 2. Parameters of T cell mediated immunity to commensal micro-organisms in patients with chronic purulent rhinosinusitis: a comparison between delayed type hypersensitivity skin test, lymphocyte transformation test and macrophage migration inhibition factor assay. (nih.gov)
- Surprisingly, neither pharmacological inhibition nor genetic ablation of CB2 had any effect on CB2 agonist-induced macrophage chemotaxis. (ox.ac.uk)
- Tumor cell culture and conditioned media from cell culture were used to perform macrophage (RAW264.7) cell migration assays, including the 129:Stat1 -/- -derived SSM2 cells as well as control Met1 and NDL tumor cells and EpH4 normal cells. (escholarship.org)
- No functional effects on phagocytic (changes in enzyme secretions not altered, chemotactic migration of granulocytes, macrophage migration, carbon clearance in vivo ) or tumor cells (growth rate, metastasis) can be detected in animals. (nih.gov)
- Our studies are specifically interested in examining the signaling occurring between tumor cells and macrophages. (yu.edu)
- Previous studies have established the presence of paracrine signaling between breast cancer cells and macrophages, where colony stimulating factor 1 (CSF-1) produced by the tumor cells stimulates the production of epidermal growth factor (EGF) by macrophages, leading to chemotactic invasion of the tumor cells. (yu.edu)
- In addition to this paracrine loop signaling between tumor cells and macrophages, it has been seen that macrophage expression of ErbB3, a member of the EGFR family of receptor tyrosine kinases, may play a role in facilitating tumor cell invasion. (yu.edu)
- This assay uses transwells coated with matrigel and endothelial cells in order to mimic the entry of tumor cells into blood vessels. (yu.edu)
- Additionally, reduction of expression of the ErbB3 receptor ligand Neuregulin1 in tumor cells using shRNA yields a similar result. (yu.edu)
- Overall our studies look to further examine the interaction between tumor cells and macrophages, and these observations indicate that ErbB3, NRG1, and JAG1 could all serve as novel targets in metastasis and the tumor microenvironment. (yu.edu)
- The key scientific breakthrough was the discovery of a pathway by which solid tumor cells acquire the ability to metastasize or leave the primary tumor to infiltrate other parts of the body. (medgadget.com)
- If you did not treat it, the tumor would grow and expand in the breast but in only about one-third of patients would the tumor cells spread through the blood stream to other parts of the body. (medgadget.com)
- Taken together our results conclusively demonstrate that CB2 is not a chemoattractant receptor for murine macrophages. (ox.ac.uk)
- Ethanol feeding for 4 days increased apoptosis of hepatic macrophages and activated complement in both wildtype and MIF-/- mice. (nih.gov)
- We hypothesized that CD14 plays a crucial role in adventitial macrophage precursor recruitment early during AAA formation.CD14(-/-) mice were resistant to AAA formation induced by 2 different AAA induction models: aortic elastase infusion and systemic angiotensin II (AngII) infusion. (nih.gov)
- MSCs and macrophages were isolated from 10 to 12 week-old BALB/c male mice . (bvsalud.org)
- Broad-spectrum CC-chemokine blockade by gene transfer inhibits macrophage recruitment and atherosclerotic plaque formation in apolipoprotein E-knockout mice. (ox.ac.uk)
- CONCLUSIONS: These findings show that a single intravenous injection of a recombinant adenovirus encoding the broad-spectrum CC-CK inhibitor 35K can reduce atherosclerosis by inhibiting CC-CK-induced macrophage recruitment in atherosclerotic ApoE KO mice. (ox.ac.uk)
- These breast cancer cells expressing NRG1 shRNA also show a significant reduction in intravasation when injected orthotopically in mice. (yu.edu)
- For IHC assays, we used the EnVision FLEX HRP Magenta, High pH (Dako Omnis) kit (Agilent Technologies, https://www.agilent.com ) with murine hyperimmune serum from mice infected with Leishmania braziliensis . (cdc.gov)
- Some specific applications in- Primary mouse hepatocytes were labeled with both su- clude stem cell tracking to damaged myocardium (1), early perparamagnetic and fluorescent agents, and transplanted detection of tissue rejection (2), early detection of cancer into the spleens of recipient mice. (nih.gov)
- CD14 directs adventitial macrophage precursor recruitment: role in early abdominal aortic aneurysm formation. (nih.gov)
- Recruitment of macrophage precursors to the adventitia plays a key role in the pathogenesis of abdominal aortic aneurysms (AAAs), but molecular mechanisms remain undefined. (nih.gov)
- Mesenchymal stromal cells (MSCs) are not only involved in osteogenesis but also help direct the recruitment of macrophages during bone regeneration via MSC- macrophage crosstalk. (bvsalud.org)
- The result appears to be recruitment of the immune reaction to the periphery of the tumor, with exclusion of immune cell infiltration into the tumor. (escholarship.org)
- The obvious candidate receptors GPR18 and GPR55 could not mediate JWH133 or HU308-induced cytoskeletal rearrangement or JWH133-induced β-arrestin recruitment in cells transfected with either receptor, demonstrating that neither are the unidentified GPCR. (ox.ac.uk)
- Osteoclastic resorption is regulated by a number of processes, including the proliferation, differentiation and recruitment of osteoclast precursors, the migration and activation of mature osteoclasts, and programmed cell death. (biomedcentral.com)
- Alveolar macrophages are the most abundant immune cell type in the lung, and these cells serve as one of the first immune responders against inhaled pathogens, particles, stimuli, and chemical allergens such as dNCOs. (cdc.gov)
- Most of those depositing in the gas-exchange region will be phagocytized by alveolar macrophages and cleared to and through the mucociliary escalator within a few weeks. (cdc.gov)
- Other particles may be engulfed by epithelial cells, primarily in the vicinity of the bronchial-alveolar duct junctions, and retained for much longer periods, with gradual removal to lymph nodes. (cdc.gov)
- However, acute (1 day) but not sustained (7 days) stimulation with CCL2 increased the alizarin red-positive area when MSCs were co-cultured with macrophages (p (bvsalud.org)
- We demonstrated that acute CCL2 stimulation promoted subsequent osteogenesis in co-culture of MSCs and macrophages . (bvsalud.org)
- 50. Renal cell carcinoma: tumor membrane lymphocyte stimulation assay. (nih.gov)
- Stimulation of macrophages with NRG1 leads to increased expression of Jagged1 (JAG1), a ligand of the Notch receptor. (yu.edu)
- Enhanced differentiated THP-1 macrophages were prepared using media containing 100 nM phorbol 12-myristate 13-acetate (PMA) to induce differentiation for 3 days and then enhanced by refeeding fresh media after removing PMA containing media for additional 3 days. (cdc.gov)
- Neutrophil differentiation was prepared using HL-60 cells cultured in complete RPMI-1640 media containing 1.5% DMSO for 7 days. (cdc.gov)
- Eosinophil differentiation was prepared using HL-60_C15 cells cultured in complete RPMI-1640 media containing 0.5 mM butyric acid for 7 days. (cdc.gov)
- Osteogenic differentiation assays were performed using MSCs with or without macrophages in co-culture . (bvsalud.org)
- Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. (nih.gov)
- It is central to a variety of different pathologic and physiologic processes across many disciplines of biology including wound healing, cancer, inflammation, cell growth and differentiation [ 3 , 4 ]. (mdpi.com)
- Cyclosporine also inhibits lymphokine production and release including interleukin-2 or T-cell growth factor (TCGF). (nih.gov)
- With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. (mdpi.com)
- Description: Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their environment. (bioinfolab.org)
- Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases. (bioinfolab.org)
- 6. Coexistence of a chemotactic factor and a retroviral P15E-related chemotaxis inhibitor in human tumor cell culture supernatants. (nih.gov)
- 7. Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. (nih.gov)
- Primary Macrophage Chemotaxis Induced by Cannabinoid Receptor 2 Agonists Occurs Independently of the CB2 Receptor. (ox.ac.uk)
- Using a real-time chemotaxis assay and a panel of chemically diverse and widely used CB2 agonists, we set out to examine whether CB2 modulates primary murine macrophage chemotaxis. (ox.ac.uk)
- As chemotaxis was pertussis toxin sensitive in both WT and CB2(-/-) macrophages, we concluded that a non-CB1/CB2, Gi/o-coupled GPCR must be responsible for CB2 agonist-induced macrophage migration. (ox.ac.uk)
- In contrast, using both laser ablation and a novel wounding assay that allows localized treatment with inhibitory drugs, we show that PI3K is essential for hemocyte chemotaxis toward wounds and that Pvf signals and PDGF/VEGF receptor expression are not required for this rapid chemotactic response. (bris.ac.uk)
- 57. Cellular and humoral immune responses in patients with metastatic renal cell carcinoma after vaccination with antigen pulsed dendritic cells. (nih.gov)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- In this study, we show that the function of KLF5 in epithelial-mesenchymal transition (EMT) and migration of liver cancer cells depends on the status of the cellular tumor antigen p53 (p53). (datacite.org)
- Efforts are therefore needed to identify novel cellular therapies consisting of expanded populations of regenerative cells that have the ability to promote therapeutic angiogenesis in advanced disease states. (biomedcentral.com)
- An incompletely understood interaction exists between the critical cellular elements-endothelial cells, smooth muscle cells, platelets, and leucocytes-of the atherosclerotic lesion. (medscape.com)
- The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines. (bioinfolab.org)
- are a rich source of dietary fiber and (poly)phenols with gastrointestinal and immune health-promoting properties, however, their mechanisms of action on the intestinal epithelial cells and transient tissue macrophages remain to be elucidated. (mdpi.com)
- In this study, we evaluated the individual effects of anthocyanins, short-chain fatty acids (metabolites derived from fiber), and a series of hydroxycinnamic and hydroxybenzoic acid metabolites common to anthocyanins and other polyphenols on epithelial gut homeostasis in human colon epithelial CCD-18 cells and murine RAW 264.7 macrophages. (mdpi.com)
- abstract = "Drosophila melanogaster hemocytes are highly motile macrophage-like cells that undergo a stereotypic pattern of migration to populate the whole embryo by late embryogenesis. (bris.ac.uk)
- CD14 gene deletion led to reduced aortic macrophage infiltration and diminished elastin degradation. (nih.gov)
- 43. Macrophage electrophoretic migration (MEM) test for lymphocyte sensitization: some practical experiences in macrophage selection. (nih.gov)
- Knockout of JAG1 in macrophages leads to a significant decrease in macrophage induced transendothelial migration. (yu.edu)
- Skeletal muscle injuries are associated with local infiltration of large numbers of mononuclear cell, degeneration of the injured myofibers and removal of the cell debris by phagocytosis. (cdc.gov)
- CCL2 promotes osteogenesis by facilitating macrophage migration during acute inflammation. (bvsalud.org)
- Genetic manipulation did not affect cell proliferation . (bvsalud.org)
- MIF has been reported to play a central role in tumor cell proliferation and invasion in several cancers. (elsevier.com)
- AG requires multiple synchronous processes that include EC proliferation, migration, and vessel stabilization. (elsevierpure.com)
- HUVECs stimulated by ixmyelocel-T exhibited enhanced migration, proliferation, and branch formation. (biomedcentral.com)
- The expression of miR‑25 and HMGB1 in serum and peripheral blood mononuclear cells (PBMCs) was compared. (spandidos-publications.com)
- However, the percentage of regenerative cells in bone marrow mononuclear cells (BMMNCs) is small, and large amounts of BMMNCs are required. (biomedcentral.com)
- Unfortunately, the percentage of regenerative cells in bone marrow mononuclear cells (BMMNCs) is small, and a large amount of BMMNCs is required in order to induce restorative angiogenesis [ 6 ]. (biomedcentral.com)
- Front Cell Dev Biol;11: 1213641, 2023. (bvsalud.org)
- The expression levels of miR‑25, HMGB1, phosphorylated (p‑)p65, tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6) and HMGB‑1 were compared, and the migration ability of cells was investigated by Transwell assays. (spandidos-publications.com)
- To further determine the effects on endothelial cells, ixmyelocel-T was co-cultured with human umbilical vein endothelial cells (HUVEC) in non-contacting Transwell® inserts. (biomedcentral.com)
- Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins (Perrot et al. (nih.gov)
- This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays. (mdpi.com)
- SARS-CoV-2 infects host cells via the binding of viral Spike (S) protein to transmembrane receptor, angiotensin-converting enzyme 2 (ACE2). (bvsalud.org)
- The combination of CCL2, MSCs, and macrophages could be a potential strategy for local cell therapy in compromised bone healing. (bvsalud.org)
- Ixmyelocel-T contains expanded populations of mesenchymal stromal cells (MSCs) and M2-like macrophages, as well as many of the CD45+ cells found in the bone marrow. (biomedcentral.com)
- In addition, we investigate tumor progression in the 129:Stat1 -/- host compared with wild-type 129/SvEv, and we describe the immune cell reaction to the tumors. (escholarship.org)
- Progression to invasive carcinoma is accompanied by a marked local stromal and immune cell response composed predominantly of T cells and macrophages. (escholarship.org)
- Activation of CB2 has been demonstrated to induce directed immune cell migration. (ox.ac.uk)
- 53. Metastatic renal cell carcinoma to the bladder: a clinicopathologic and immunohistochemical study. (nih.gov)
- Endothelial cells (ECs) are an integral part of AG and are sites of HCMV persistence. (elsevierpure.com)
- Simple Modifications to Methimazole that Enhance its Inhibitory Effect on Tumor Necrosis Factor-alpha-Induced Vascular Cell Adhesion Molecule-1 Expression by Human Endothelial Cells. (ohio.edu)
- This work demonstrates that ixmyelocel-T interacts with endothelial cells in a paracrine manner, resulting in angiogenesis and endothelial protection. (biomedcentral.com)
- In the clinical setting, the levels of many immune mediators produced by macrophages have been found elevated in the asthmatic airway. (cdc.gov)
- however, whether this mechanism participates in regulation of other asthma-associated mediators secreted by macrophages/BALCs after MDI exposure is currently unknown. (cdc.gov)
- The first aim of this study was to identify candidate asthma-associated, macrophage-secreted mediators that can be regulated after MDI exposure. (cdc.gov)
- Regulatory mechanisms involve soluble mediators, cell-cell and cell-matrix interactions. (biomedcentral.com)
- TaqMan gene expression assays were obtained from ThermoFisher Scientific. (cdc.gov)
- Using gene expression methodology (RNAase protection assay and real-time PCR) in a mouse model (freeze injury of Tibialis anterior muscle, TA)t we demonstrated an expression of MCP-l and MIP-l p in the first 24 hrs postinjury. (cdc.gov)
- It also reduced MUC5AC, proinflammatory cytokine, and chemokine gene expression and inhibited ICAM-1 expression for monocyte adhesion in IL-1β-stimulated A549 cells. (oncotarget.com)
- In parallel with these events, quiescent muscle precursor cells (satellite cells), are activated. (cdc.gov)
- Plasmid DNAs were transfected into THP-1 macrophages using Mirus TransIT-2020 transfection reagent according to manufacturer's instructions. (cdc.gov)
- miR-inhibitors were transfected into THP-1 macrophages using Lipofectamine RNAiMAX transfection reagent according to manufacturer's instructions. (cdc.gov)
- Following transfection with miR‑25 mimics and/or short interfering RNA‑HMGB1, the expression of HMGB1 in macrophages decreased significantly, the expression of p‑p65, HMGB‑1, TNF‑α and IL‑6 in the culture solution were also decreased, and the migration ability of macrophages was attenuated. (spandidos-publications.com)
- CytoSelect Cell Migration Assays are ideal for determining the chemotactic properties of cells. (bioinfolab.org)
- Using in vitroAG assays, neutralization of IL-6 significantly reduced neovessel formation. (elsevierpure.com)
- Furthermore, zinc finger E-box-binding homeobox 2 (ZEB2) is the main regulator of KLF5 in EMT in liver cancer cells in the context of p53 loss. (datacite.org)
- In this study we used a well developed model for liver determine whether the observed contrast could have been due cell transplantation to demonstrate that single-cell detec- to dead cells or free particles, and the results confirmed that tion in vivo can be achieved with MRI. (nih.gov)
- Noninvasive imaging of single cells in intact, live organ- sue damage is present in the liver. (nih.gov)
- Cells can then migrate isms would have an enormous impact in all fields in- out of the spleen to the liver, where they engraft. (nih.gov)
- The results mentation of MRI and augmenting the relaxivities and deliv- indicate that single cells can be detected in the liver. (nih.gov)
- This study investigated the use of CCL2 as a therapeutic target for local cell therapy . (bvsalud.org)
- In conclusion, ixmyelocel-T therapy may provide a new aspect of therapeutic angiogenesis in this patient population where expanded populations of regenerative cells might be required. (biomedcentral.com)
- 10. In vivo effects of thymostimulin treatment on monocyte polarization, dendritic cell clustering and serum p15E-like trans-membrane factors in operable head and neck squamous cell carcinoma patients. (nih.gov)
- to macrophages (2), and some initial reports have described labeled hepatocyte phantoms in combination with histological in vivo labeling of peripheral T cells (16) and neural stem evaluation confirmed the presence of dispersed single hepato- cells (17). (nih.gov)
- The 12 µm pore size is suitable for astrocytes and other large or slow-moving cells. (bioinfolab.org)
- CCRl and CCR5, were colocalized, through immunostaining analysis, with mac-3, a marker of activated macrophages and myogenin, a transcription factor involved in satellite cell activation. (cdc.gov)
- However, the ability of CB2 to act as a chemoattractant receptor in macrophages remains largely unexplored. (ox.ac.uk)
- The Migration Assay Bioteck reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bioinfolab.org)
- Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (bionotatki.com)
- VWR provides the cell culture community with access to the most reliable supply of exceptional quality Fetal Bovine Serum: VWR Life Science Seradigm. (vwr.com)
- The present study suggests that miR‑25 attenuated the induction of HMGB1 by LPS, decreased the activity of nuclear factor‑κB and the transcriptional activation of TNF‑α and IL‑6, and suppressed the migration of macrophages. (spandidos-publications.com)
- We used a single cell assay to demonstrate that macrophage-colony stimulating factor (M-CSF) and transforming growth factor beta are chemotaxins for mammalian osteoclasts. (biomedcentral.com)
- whereas it did not inhibit migration induced by transforming growth factor beta [ 3 ]. (biomedcentral.com)
- Our results demonstrate that at least two separate mechanisms operate in D. melanogaster embryos to direct hemocyte migration and show that although PI3K is crucial for hemocytes to sense a chemotactic gradient from a wound, it is not required to sense the growth factor signals that coordinate their developmental migrations along the ventral midline during embryogenesis. (bris.ac.uk)
- Krüppel-like factor 5 (KLF5) can both promote and suppress cell migration, but the underlying mechanisms have not been elucidated. (datacite.org)
- We show that using an ErbB3 blocking antibody results in a significant reduction of macrophage-induced transendothelial migration of breast cancer cells. (yu.edu)
- 4. Defects in monocyte polarization and dendritic cell clustering in patients with Graves' disease. (nih.gov)