Cell Migration Assays
Cell Migration Assays, Macrophage
Cell Migration Assays, Leukocyte
Cell Migration Inhibition
RNA, Small Interfering
rac1 GTP-Binding Protein
Focal Adhesion Protein-Tyrosine Kinases
Reverse Transcriptase Polymerase Chain Reaction
Gene Knockdown Techniques
Matrix Metalloproteinase 9
Gene Expression Regulation, Neoplastic
Diffusion Chambers, Culture
Matrix Metalloproteinase 2
rac GTP-Binding Proteins
Tumor Cells, Cultured
Cell Surface Extensions
Cell Adhesion Molecules
Platelet-Derived Growth Factor
Vascular Endothelial Growth Factor A
rhoA GTP-Binding Protein
Proto-Oncogene Proteins c-akt
Myocytes, Smooth Muscle
rho GTP-Binding Proteins
Focal Adhesion Kinase 1
Dose-Response Relationship, Drug
cdc42 GTP-Binding Protein
Molecular Sequence Data
Macrophage Migration-Inhibitory Factors
Culture Media, Conditioned
Epidermal Growth Factor
Transendothelial and Transepithelial Migration
Real-Time Polymerase Chain Reaction
Gene Expression Regulation
Intercellular Signaling Peptides and Proteins
Amino Acid Sequence
Proto-Oncogene Proteins c-met
Crk-Associated Substrate Protein
Fibroblast Growth Factor 2
Mitogen-Activated Protein Kinase 3
Disease Models, Animal
NIH 3T3 Cells
Extracellular Signal-Regulated MAP Kinases
Mesenchymal Stromal Cells
Gene Expression Regulation, Developmental
MAP Kinase Signaling System
The novel proangiogenic effect of hydrogen sulfide is dependent on Akt phosphorylation. (1/100)OBJECTIVE: Hydrogen sulfide (H(2)S) has been reported to be a gasotransmitter which regulates cardiovascular homeostasis. The present study aims to examine the hypothesis that hydrogen sulfide is able to promote angiogenesis. METHODS: Angiogenesis was assessed using in vitro parameters (i.e. endothelial cell proliferation, adhesion, transwell migration assay, scratched wound healing and formation of tube-like structure) and in vivo by assessing neovascularization in mice. Phosphorylation of Akt was measured using Western blot analysis. RESULTS: Exogenously administered NaHS (H(2)S donor) concentration-dependently (10-20 micromol/l) increased cell growth, migration, scratched wound healing and tube-like structure formation in cultured endothelial cells. These effects of NaHS on endothelial wound healing and tube-like structure formation were prevented by either the phosphatidylinositol 3-kinase (PI3K) inhibitor LY 294002 (5 micromol/l) or transfection of a dominant-negative mutant of Akt. NaHS increased Akt phosphorylation and this effect was also blocked by either LY 294002 or wortmannin (25 nmol/l). NaHS did not significantly alter the levels of vascular endothelial growth factor, mRNA expression of fibroblast growth factor and angiopoietin-1, or nitric oxide metabolites. NaHS treatment (10 and 50 micromol kg(-1) day(-1)) significantly promoted neovascularization in vivo in mice. CONCLUSION: The present study reports a novel proangiogenic role of H(2)S which is dependent on activation of Akt. (+info)
A rapid screening method for population-specific neuronal motogens, substrates and associated signaling pathways. (2/100)We developed and characterized an assay that allows for rapid examination of migration of specific neuronal populations within a mixed population using the Boyden chamber principle. Migration of cerebellar interneurons and granule cells was examined using mice expressing enhanced green fluorescent protein (eGFP) under the glutamate decarboxylase (GAD(65)) and growth-associated protein-43 (GAP43) promoters, respectively. Brain-derived neurotrophic factor (BDNF) was used as the prototypic motogen for both populations. Fluorescent light-blocking inserts (FluoroBlok) with different pore sizes and densities were compared in a two-compartment assay. Immunodetection of polarity markers and nuclear staining indicated that dendrites and somata are preferentially extended through the pores in response to BDNF. Inserts coated with extracellular matrix (ECM) proteins were used to examine interactions between BDNF and the ECM during migration. ECM proteins alone stimulated migration when the lower side of the insert was coated, however coating of both sides of the insert slowed migration when compared to poly-D-lysine. Addition of a PI 3-kinase inhibitor to the lower compartment blocked BDNF-stimulated migration of both populations while a Src inhibitor reduced laminin-stimulated migration of interneurons, but not granule cells. We also examined use of neurons cultured from GAD(65)-eGFP mice as a reporter system for promoter activity. GAD(65)-eGFP mice may also be useful as a model for promoter regulation and the potential confounding effects of eGFP induction by the stimuli are also addressed. This assay allows for rapid analysis of motogens, substrates and signaling pathways that regulate migration of selected neuronal populations. (+info)
Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. (3/100)Adult human bone marrow-derived mesenchymal stem cells (hMSCs) are under study as therapeutic delivery agents that assist in the repair of damaged tissues. To achieve the desired clinical outcomes for this strategy requires a better understanding of the mechanisms that drive the recruitment, migration, and engraftment of hMSCs to the targeted tissues. It is known that hMSCs are recruited to sites of stress or inflammation to fulfill their repair function. It is recognized that toll-like receptors (TLRs) mediate stress responses of other bone marrow-derived cells. This study explored the role of TLRs in mediating stress responses of hMSCs. Accordingly, the presence of TLRs in hMSCs was initially established by reverse transcription-polymerase chain reaction assays. Flow cytometry and fluorescence immunocytochemical analyses confirmed these findings. The stimulation of hMSCs with TLR agonists led to the activation of downstream signaling pathways, including nuclear factor kappaB, AKT, and MAPK. Consequently, activation of these pathways triggered the induction and secretion of cytokines, chemokines, and related TLR gene products as established from cDNA array, immunoassay, and cytokine antibody array analyses. Interestingly, the unique patterns of affected genes, cytokines, and chemokines measured identify these receptors as critical players in the clinically established immunomodulation observed for hMSCs. Lastly, hMSC migration was promoted by TLR ligand exposure as demonstrated by transwell migration assays. Conversely, disruption of TLRs by neutralizing TLR antibodies compromised hMSC migration. This study defines a novel TLR-driven stress and immune modulating response for hMSCs that is critical to consider in the design of stem cell-based therapies. (+info)
Contribution of lung fibroblast migration in the fibrotic process of airway remodeling in asthma. (4/100)(+info)
Immunologic activation of human syncytiotrophoblast by Plasmodium falciparum. (5/100)(+info)
Altered chemotactic response of myeloid and plasmacytoid dendritic cells from patients with chronic hepatitis C: role of alpha interferon. (6/100)(+info)
Collagen density promotes mammary tumor initiation and progression. (7/100)(+info)
Lipopolysaccharide induces macrophage migration via prostaglandin D(2) and prostaglandin E(2). (8/100)(+info)
1. Tumor size and location: Larger tumors that have spread to nearby tissues or organs are generally considered more invasive than smaller tumors that are confined to the original site.
2. Cellular growth patterns: The way in which cancer cells grow and divide can also contribute to the overall invasiveness of a neoplasm. For example, cells that grow in a disorganized or chaotic manner may be more likely to invade surrounding tissues.
3. Mitotic index: The mitotic index is a measure of how quickly the cancer cells are dividing. A higher mitotic index is generally associated with more aggressive and invasive cancers.
4. Necrosis: Necrosis, or the death of cells, can be an indication of the level of invasiveness of a neoplasm. The presence of significant necrosis in a tumor is often a sign that the cancer has invaded surrounding tissues and organs.
5. Lymphovascular invasion: Cancer cells that have invaded lymphatic vessels or blood vessels are considered more invasive than those that have not.
6. Perineural invasion: Cancer cells that have invaded nerve fibers are also considered more invasive.
7. Histological grade: The histological grade of a neoplasm is a measure of how abnormal the cancer cells look under a microscope. Higher-grade cancers are generally considered more aggressive and invasive than lower-grade cancers.
8. Immunohistochemical markers: Certain immunohistochemical markers, such as Ki-67, can be used to evaluate the proliferative activity of cancer cells. Higher levels of these markers are generally associated with more aggressive and invasive cancers.
Overall, the degree of neoplasm invasiveness is an important factor in determining the likelihood of the cancer spreading to other parts of the body (metastasizing) and in determining the appropriate treatment strategy for the patient.
Foreign-body migration refers to the movement or migration of a foreign object or material within the body over time. This can occur after a surgical procedure, injury, or other medical intervention where a foreign object is introduced into the body. The term "foreign body" includes any object or material that is not naturally present within the body, such as implants, sutures, staples, and other medical devices.
The migration of a foreign body can occur due to various factors, including:
1. Mechanical forces: Movement of the body, such as during exercise or daily activities, can cause the foreign object to shift position or migrate to another part of the body.
2. Biological forces: The body's natural healing processes and inflammatory responses can cause the foreign object to move or change shape over time.
3. Chemical forces: Corrosion or degradation of the foreign material can lead to its migration within the body.
4. Cellular forces: Cells in the body can surround and interact with the foreign object, leading to its movement or displacement.
The migration of a foreign body can have significant clinical implications, including:
1. Pain and discomfort: The movement of a foreign object within the body can cause pain, discomfort, and inflammation.
2. Infection: The migration of a foreign object can increase the risk of infection, particularly if the object is made of a material that is susceptible to bacterial growth.
3. Organ damage: If the migrated foreign object damages surrounding tissues or organs, it can lead to serious complications and long-term health problems.
4. Revision surgery: In some cases, the migration of a foreign body may require revision surgery to remove or reposition the object.
To prevent foreign-body migration, medical professionals use various techniques, such as:
1. Implant fixation: Implants can be fixed in place using bone screws, sutures, or other fixation devices to minimize their movement.
2. Biocompatible materials: Using biocompatible materials for implants and other medical devices can reduce the risk of foreign-body reaction and migration.
3. Proper surgical technique: Surgeons must use proper surgical techniques when inserting foreign objects into the body, such as using a sterile environment and appropriate insertion angles.
4. Postoperative care: Proper postoperative care, including antibiotics and pain management, can help prevent complications and promote healing.
Overall, preventing the migration of foreign bodies is essential to ensure successful medical outcomes and minimize the risk of complications.
Neoplastic metastasis can occur in any type of cancer but are more common in solid tumors such as carcinomas (breast, lung, colon). It is important for cancer diagnosis and prognosis because metastasis indicates that the cancer has spread beyond its original site and may be more difficult to treat.
Metastases can appear at any distant location but commonly found sites include the liver, lungs, bones, brain, and lymph nodes. The presence of metastases indicates a higher stage of cancer which is associated with lower survival rates compared to localized cancer.
Pathologic neovascularization can be seen in a variety of conditions, including cancer, diabetic retinopathy, and age-related macular degeneration. In cancer, for example, the formation of new blood vessels can help the tumor grow and spread to other parts of the body. In diabetic retinopathy, the growth of new blood vessels in the retina can cause vision loss and other complications.
There are several different types of pathologic neovascularization, including:
* Angiosarcoma: a type of cancer that arises from the cells lining blood vessels
* Hemangiomas: benign tumors that are composed of blood vessels
* Cavernous malformations: abnormal collections of blood vessels in the brain or other parts of the body
* Pyogenic granulomas: inflammatory lesions that can form in response to trauma or infection.
The diagnosis of pathologic neovascularization is typically made through a combination of physical examination, imaging studies (such as ultrasound, CT scans, or MRI), and biopsy. Treatment options vary depending on the underlying cause of the condition, but may include medications, surgery, or radiation therapy.
In summary, pathologic neovascularization is a process that occurs in response to injury or disease, and it can lead to serious complications. It is important for healthcare professionals to be aware of this condition and its various forms in order to provide appropriate diagnosis and treatment.
There are several types of gliomas, including:
1. Astrocytoma: This is the most common type of glioma, accounting for about 50% of all cases. It arises from the star-shaped cells called astrocytes that provide support and nutrients to the brain's nerve cells.
2. Oligodendroglioma: This type of glioma originates from the oligodendrocytes, which are responsible for producing the fatty substance called myelin that insulates the nerve fibers.
3. Glioblastoma (GBM): This is the most aggressive and malignant type of glioma, accounting for about 70% of all cases. It is fast-growing and often spreads to other parts of the brain.
4. Brain stem glioma: This type of glioma arises in the brain stem, which is responsible for controlling many of the body's vital functions such as breathing, heart rate, and blood pressure.
The symptoms of glioma depend on the location and size of the tumor. Common symptoms include headaches, seizures, weakness or numbness in the arms or legs, and changes in personality, memory, or speech.
Gliomas are diagnosed through a combination of imaging tests such as CT or MRI scans, and tissue biopsy to confirm the presence of cancer cells. Treatment options for glioma depend on the type and location of the tumor, as well as the patient's overall health. Surgery is often the first line of treatment to remove as much of the tumor as possible, followed by radiation therapy and/or chemotherapy to kill any remaining cancer cells.
The prognosis for glioma patients varies depending on the type and location of the tumor, as well as the patient's overall health. In general, the prognosis is better for patients with slow-growing, low-grade tumors, while those with fast-growing, high-grade tumors have a poorer prognosis. Overall, the 5-year survival rate for glioma patients is around 30-40%.
There are different types of Breast Neoplasms such as:
1. Fibroadenomas: These are benign tumors that are made up of glandular and fibrous tissues. They are usually small and round, with a smooth surface, and can be moved easily under the skin.
2. Cysts: These are fluid-filled sacs that can develop in both breast tissue and milk ducts. They are usually benign and can disappear on their own or be drained surgically.
3. Ductal Carcinoma In Situ (DCIS): This is a precancerous condition where abnormal cells grow inside the milk ducts. If left untreated, it can progress to invasive breast cancer.
4. Invasive Ductal Carcinoma (IDC): This is the most common type of breast cancer and starts in the milk ducts but grows out of them and invades surrounding tissue.
5. Invasive Lobular Carcinoma (ILC): It originates in the milk-producing glands (lobules) and grows out of them, invading nearby tissue.
Breast Neoplasms can cause various symptoms such as a lump or thickening in the breast or underarm area, skin changes like redness or dimpling, change in size or shape of one or both breasts, discharge from the nipple, and changes in the texture or color of the skin.
Treatment options for Breast Neoplasms may include surgery such as lumpectomy, mastectomy, or breast-conserving surgery, radiation therapy which uses high-energy beams to kill cancer cells, chemotherapy using drugs to kill cancer cells, targeted therapy which uses drugs or other substances to identify and attack cancer cells while minimizing harm to normal cells, hormone therapy, immunotherapy, and clinical trials.
It is important to note that not all Breast Neoplasms are cancerous; some are benign (non-cancerous) tumors that do not spread or grow.
There are several types of melanoma, including:
1. Superficial spreading melanoma: This is the most common type of melanoma, accounting for about 70% of cases. It usually appears as a flat or slightly raised discolored patch on the skin.
2. Nodular melanoma: This type of melanoma is more aggressive and accounts for about 15% of cases. It typically appears as a raised bump on the skin, often with a darker color.
3. Acral lentiginous melanoma: This type of melanoma affects the palms of the hands, soles of the feet, or nail beds and accounts for about 5% of cases.
4. Lentigo maligna melanoma: This type of melanoma usually affects the face and is more common in older adults.
The risk factors for developing melanoma include:
1. Ultraviolet (UV) radiation exposure from the sun or tanning beds
2. Fair skin, light hair, and light eyes
3. A history of sunburns
4. Weakened immune system
5. Family history of melanoma
The symptoms of melanoma can vary depending on the type and location of the cancer. Common symptoms include:
1. Changes in the size, shape, or color of a mole
2. A new mole or growth on the skin
3. A spot or sore that bleeds or crusts over
4. Itching or pain on the skin
5. Redness or swelling around a mole
If melanoma is suspected, a biopsy will be performed to confirm the diagnosis. Treatment options for melanoma depend on the stage and location of the cancer and may include surgery, chemotherapy, radiation therapy, or a combination of these. Early detection and treatment are key to successful outcomes in melanoma cases.
In conclusion, melanoma is a type of skin cancer that can be deadly if not detected early. It is important to practice sun safety, perform regular self-exams, and seek medical attention if any suspicious changes are noticed on the skin. By being aware of the risk factors, symptoms, and treatment options for melanoma, individuals can take steps to protect themselves from this potentially deadly disease.
1) They share similarities with humans: Many animal species share similar biological and physiological characteristics with humans, making them useful for studying human diseases. For example, mice and rats are often used to study diseases such as diabetes, heart disease, and cancer because they have similar metabolic and cardiovascular systems to humans.
2) They can be genetically manipulated: Animal disease models can be genetically engineered to develop specific diseases or to model human genetic disorders. This allows researchers to study the progression of the disease and test potential treatments in a controlled environment.
3) They can be used to test drugs and therapies: Before new drugs or therapies are tested in humans, they are often first tested in animal models of disease. This allows researchers to assess the safety and efficacy of the treatment before moving on to human clinical trials.
4) They can provide insights into disease mechanisms: Studying disease models in animals can provide valuable insights into the underlying mechanisms of a particular disease. This information can then be used to develop new treatments or improve existing ones.
5) Reduces the need for human testing: Using animal disease models reduces the need for human testing, which can be time-consuming, expensive, and ethically challenging. However, it is important to note that animal models are not perfect substitutes for human subjects, and results obtained from animal studies may not always translate to humans.
6) They can be used to study infectious diseases: Animal disease models can be used to study infectious diseases such as HIV, TB, and malaria. These models allow researchers to understand how the disease is transmitted, how it progresses, and how it responds to treatment.
7) They can be used to study complex diseases: Animal disease models can be used to study complex diseases such as cancer, diabetes, and heart disease. These models allow researchers to understand the underlying mechanisms of the disease and test potential treatments.
8) They are cost-effective: Animal disease models are often less expensive than human clinical trials, making them a cost-effective way to conduct research.
9) They can be used to study drug delivery: Animal disease models can be used to study drug delivery and pharmacokinetics, which is important for developing new drugs and drug delivery systems.
10) They can be used to study aging: Animal disease models can be used to study the aging process and age-related diseases such as Alzheimer's and Parkinson's. This allows researchers to understand how aging contributes to disease and develop potential treatments.
Time Lapse Analysis of Cell Migration and Proliferation in Scratch Assay | Yokogawa Romania
Essential Oil of |i|Bursera morelensis|/i| Promotes Cell Migration on Fibroblasts: In Vitro Assays. | Molecules;28(17)2023 Aug...
SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair | Nature Communications
Cell migration assay I : BIOMED Labor
Frontiers | Pituitary Adenylate Cyclase Activating Polypeptide Has Inhibitory Effects on Melanoma Cell Proliferation and...
CAFs and cancer cells co-migration in 3D spheroid invasion assay | Crick
Combination of multiple neural crest migration assays to identify environmental toxicants from a proof-of-concept chemical...
Axon-like protrusions promote small cell lung cancer migration and metastasis | eLife
Single Cell Tracking for Migration Analyses | Zuse Institute Berlin (ZIB)
MicroRNA-Mediated Calcineurin Signaling Activation Induces CCL2, CCL3, CCL5, IL8 and Chemotactic Activities in 4,4'-Methylene...
Laminin 5 deposition regulates keratinocyte polarization and persistent migration | Journal of Cell Science | The Company of...
Brucine, an alkaloid from seeds of Strychnos nux-vomica Linn., represses hepatocellular carcinoma cell migration and metastasis...
NIOSHTIC-2 Search Results - Full View
VEGF Neutralization Plus CTLA-4 Blockade Alters Soluble and Cellular Factors Associated with Enhancing Lymphocyte Infiltration...
Developmental Neurotoxicity Screening 21005
Discovery of new small molecules inhibiting 67 kDa laminin receptor interaction with laminin and cancer cell invasion |...
Magnetic resonance imaging of odorant activity-dependent migration of neural precursor cells and olfactory bulb growth - PubMed
PTEN phosphatase and tensin homolog [Homo sapiens (human)] - Gene - NCBI
NOT-OD-20-028: Findings of Research Misconduct
NIAAA Director's Report on Institute Activities to the 132nd Meeting of the National Advisory Council on Alcohol Abuse and...
Cellular Dynamics of Angiogenesis - Amir H. Gandjbakhche Lab | NICHD - Eunice Kennedy Shriver National Institute of Child...
ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase ...
Software | Benny Geiger's
TMSB4X thymosin beta 4 X-linked [Homo sapiens (human)] - Gene - NCBI
Mutational and functional analysis reveals ADAMTS18 metalloproteinase as a novel driver in melanoma - PubMed
Events - School of Mathematics and Physics - University of Queensland
Ubiquitin COOH-terminal hydrolase 1: a biomarker of renal cell carcinoma associated with enhanced tumor cell proliferation and...
- PAC1 receptor was identified in melanocytes in vivo and in vitro and in melanoma cell lines as well as in melanoma lesions. (frontiersin.org)
- Cell-cell contacts ( via E-cadherin) between melanocytes and keratinocytes play a key role in regulation of melanocyte proliferation and behaviour with soluble factors being less important, a phenomenon that has been investigated both in vivo and in vitro ( 3 ). (frontiersin.org)
- IL1α and TNFα induced expression of E-selectin, CXCL1, and VCAM1 on melanoma tumor-associated endothelial cells (TEC) in vitro and promoted adhesion of activated T cells onto TEC. (aacrjournals.org)
- Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro. (nih.gov)
- We therefore assessed the effects of PFAS on trophoblast migration, invasion, and gene expression in vitro. (nih.gov)
- In vitro , gelsolin deficiency did not affect proliferation or neuronal differentiation of adult neural progenitors cells (NPCs) but resulted in retarded migration. (jneurosci.org)
- Caruntu C, Boda D, Constantin C, Caruntu A and Neagu M: Catecholamines increase in vitro proliferation of murine B16F10 melanoma cells. (spandidos-publications.com)
- In vitro experiments demonstrated that over-expression of CLCA4 could inhibit cell migration and invasion by suppressing epithelial-mesenchymal transition (EMT) via PI3K/ATK signaling and change the expression patterns of EMT markers in CLCA4-gain-of-function cell models. (medscimonit.com)
- Immunosuppression mediated by MICCop was investigated in vivo by daily assessment of clinical signs of paralysis and in in vitro restimulation assays of peripheral immune cells. (uni-wuerzburg.de)
- The number and inhibitory activity of CD4+CD25+FoxP3+ regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. (uni-wuerzburg.de)
- Humoral and cellular immune responses were then determined by ELISA and in vitro antigen restimulation assay. (uni-wuerzburg.de)
- In vitro angiogenesis assays: migration, proliferation and differentiation into tube-like structures in Matrigel™ ™ assays, have been used in human brain microvessel endothelial cells (hCMEC/D3). (bl.uk)
- In vitro cell migration assays mainly assess chemokine-triggered random migration (chemokinesis). (nih.gov)
- Using our in vitro tube formation assay by the human microvascular endothelial cells (HMVEC), we have tested the inhibitory effect of catechins on vascular endothelial growth factor (VEGF} signaling during angiogenesis. (nih.gov)
- Down-regulates macrophage migration in wound-healing assays (in vitro) (By similarity). (nih.gov)
- WIS-PhagoTracker is a software application for quantitative analysis of high throughput cell migration assay. (weizmann.ac.il)
- We describe a fluorescence-based phenotypic assay in a 1536-well plate format for high-throughput screening of novel inhibitors of chemotaxis/migration within complex libraries of thousands of compounds. (nih.gov)
- My laboratory will design and characterize molecular motor variants using a rapid testing cycle that relies on new instrumentation for high throughput single molecule tracking and manipulation assays. (nih.gov)
- This invention includes a novel high-throughput assay to identify orthosteric inhibitors blocking the Zika virus NS2B-NS3 protease. (nih.gov)
- In this study, exposure of HTR-8/SVneo cells to 100 microg/ml of SS welding fumes for 24 h using the RadiusTM Migration assay showed significant inhibition of cellular migratory ability, whereas cells exposed to MS were not affected. (cdc.gov)
- They are commonly used to measure the migration of immune cells in response to stimuli and the inhibition of immune cell migration by immunosuppressive factors. (nih.gov)
- Administration of anti-IL-8 antibody resulted in the inhibition of FAK expression, its downstream signaling, and the invasive potential of the OS cells, resulting in decrease in metastatic lesions. (biomedcentral.com)
- Furtherly, miR-940-downregulated expression was also found in HCC patients, and importantly, miR-940 inhibition reversed circACTG1 expression in 97H cells with circACTG1 knockdown. (hindawi.com)
- Catechins can contribute to cancer prevention not only by the reduction of tumor cell growth, migration and invasion, but also by the inhibition of angiogenesis, an obligatory process for tumor growth. (nih.gov)
- Here, we show that upon damage in a mouse model, epidermal cells at the wound edge convert to an embryonic-like state, altering particularly the cytoskeletal/extracellular matrix (ECM) components and differentiation program. (nature.com)
- Correspondingly, postnatal induction of SOX11 represses epidermal terminal differentiation while deficiency of Sox11 and Sox4 accelerates differentiation and dramatically impairs cell motility and re-epithelialization. (nature.com)
- Dendritic cell differentiation was prepared using undifferentiated THP-1 monocytes cultured in serum-free RPMI-1640 culture medium supplemented with 100 ng/ml rhGM-CSF, 10 ng/ml rhTNF-α, and 200 ng/ml ionomycin for 3 days. (cdc.gov)
- Neutrophil differentiation was prepared using HL-60 cells cultured in complete RPMI-1640 media containing 1.5% DMSO for 7 days. (cdc.gov)
- Eosinophil differentiation was prepared using HL-60_C15 cells cultured in complete RPMI-1640 media containing 0.5 mM butyric acid for 7 days. (cdc.gov)
- The uptake of these growth factors by capillaries initiates a sequence of events such as cell migration, cell differentiation and assembly which finally lead to the formation of a capillary network. (nih.gov)
- 9. Myeloid-derived suppressor cells contribute to systemic lupus erythaematosus by regulating differentiation of Th17 cells and Tregs. (nih.gov)
- 16. Human embryonic mesenchymal stem cells alleviate pathologic changes of MRL/Lpr mice by regulating Th7 cell differentiation. (nih.gov)
- Design: Previous findings in our lab suggests, calcitriol act as differentiation agent in human osteosarcoma cell lines 143B and SaOS-2. (ku.edu)
- Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. (nih.gov)
- This gene encodes an evolutionarily conserved protein associated with cell apoptosis. (nih.gov)
- PDCD10 promotes proliferation, migration, and invasion of osteosarcoma by inhibiting apoptosis and activating EMT pathway. (nih.gov)
- A hypoxia induced apoptosis assay was performed by seeding hCMEC/D3 on to glass coverslips in serum poor medium. (bl.uk)
- Moreover, citicoline treatment showed a decrease in number of apoptotic cells (positive PI staining) in hypoxia induced apoptosis compared to untreated cells. (bl.uk)
- Objective: Our primary objective is to investigate effects of calcitriol in combination with cisplatin on the osteosarcoma cell apoptosis, invasion and migration. (ku.edu)
- The cover features an invading spheroid in the 3D invasion assay of their study for U87MG cells transfected with si-GALNT2 1. (portlandpress.com)
- Before beginning the assay, coat the plate for the invasion assay with 50 microliters of extracellular matrix gel, diluted in ice-cold cell culture medium, to a 100 microgram per milliliter concentration. (jove.com)
- SUMMARY: Findings of research misconduct have been made against Dr. Sudhakar Yakkanti (Respondent) (formerly named Sudhakar Akulapalli),  former staff scientist and Director of the Cell Signaling, Retinal & Tumor Angiogenesis Laboratory, Boys Town National Research Hospital (BTNRH). (nih.gov)
- A major process during angiogenesis is cell migration. (nih.gov)
- CLCA4 inhibits migration and invasion by suppressing EMT via PI3K/ATK signaling and predicts favorable prognosis of CRC which may help to distinguish potential risk of lymph node metastasis in CRC. (medscimonit.com)
- My group is interested in the regulatory pathways that control the proliferation and migration of normal cells and the events that disrupt this control in tumourigenesis and metastasis. (nottingham.ac.uk)
- Cancer cell mobility is crucial for the initiation of metastasis. (jove.com)
- Understanding the molecular mechanisms that drive metastatic potential via communication by humoral factors between OS cells and hMSCs will be important for the identification of new targets for prevention of metastasis. (biomedcentral.com)
- Chemotaxis and cell migration also play pivotal roles in normal physiological processes such as embryogenesis, inflammation, and wound healing, as well as in pathological processes including chronic inflammatory disease and cancer metastasis. (nih.gov)
- The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users. (sigmaaldrich.com)
- Recently, time-lapse imaging has allowed accurate analyses of chemokinesis as well as directed tumor cell migration in a chemokine gradient (chemotaxis). (nih.gov)
- We established conditions to study chemokinesis and chemotaxis in invasive, lung colony forming breast cancer cells (MCF10CA1a) and analyzed their migratory response to EGF, LPA-, and TGF-beta1. (nih.gov)
- In EZTaxiscan chemotaxis assays, EGF mainly induced chemokinesis of MCF10CA1a cells, while LPA and TGF-beta1 gave rise to robust chemotaxis in a concentration-dependent fashion. (nih.gov)
- Aspects of innate immunity derive from characteristics inherent to phagocytes, including chemotaxis toward and engulfment of unicellular organisms or cell debris. (nih.gov)
- Novel chemotaxis/migration inhibitors are, thus, desirable for developing effective therapeutics and probing molecular mechanisms. (nih.gov)
- Although the assay utilizes the unique cellular response properties of Dictyostelium , the compounds identified are able to inhibit chemotaxis of mammalian cells. (nih.gov)
- This novel compound screening approach enables rapid identification of novel lead compounds that inhibit chemotaxis in human and other cells for drug development and research tools. (nih.gov)
- Wound healing assay and Transwell assay were carried out to access the cell migration and invasion ability. (medscimonit.com)
- Here we show that mouse and human SCLC cells in culture and in vivo can grow cellular protrusions that resemble axons. (elifesciences.org)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- During embryogenesis and placentation, cellular migration is a highly orchestrated and multi-step process that plays an integral role in providing the foundation for a successful pregnancy. (cdc.gov)
- To study the network formation of endothelial cells (EC) in an Extra-Cellular-Matrix (ECM) environment, we have devised an EC aggregation-type model based on a diffusion-limited-cluster-aggregation model (DLCA), where clusters of particles diffuse and stick together upon contact. (nih.gov)
- Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays. (sigmaaldrich.com)
- This method can provide insight into cancer cell invasion, through the extra-cellular matrix, and can be applied to other cell types. (jove.com)
- We developed a new co-culture model, using OS cells and mesenchymal stem cells (MSCs) without cellular contact, and found that both cell types expressed IL-8 at a high level, and FAK in OS cells was phosphorylated leading to an increase in the metastatic potential of the tumor in the co-culture condition. (biomedcentral.com)
- Here, we investigate the effect of long-term exposure to high aspect ratio CNTs on the aggressive behaviors of human pleural mesothelial cells, the primary cellular target of human lung mesothelioma. (cdc.gov)
- Our laboratory group studies signaling cascades essential for eukaryotic growth and development, using molecular, genetic, cellular, and biochemical techniques, and the model eukaryote Dictyostelium, which grow as individual phagocytic cells in enriched media, but develop multicellularly upon nutrient depletion. (nih.gov)
- Trophoblast migration and invasion were assessed using a modified scratch assay in the absence or presence of Matrigel, respectively. (nih.gov)
- I used IncuCyte to perform scratch migration assays and it really made my life easier comp. (selectscience.net)
- To achieve an optimal scratch time, ensure that the seeding density has been optimized and do not prolong the incubation period once the cell monolayer has reached 100 percent confluence. (jove.com)
- Specific areas of expertise include RNA sequencing and ChIP sequencing and related bioinformatics, protein-DNA interactions, cell migration and cell invasion assays, and the analysis of signal transduction pathways. (nottingham.ac.uk)
- We have defined cell autonomous and non-autonomous signal transduction pathways that drive transitional decisions to promote growth and/or regulate development. (nih.gov)
- This laboratory also investigates molecular processes required for establishing a terminally differentiated organism from a homogeneous population of totipotent cells and is defining signal transduction pathways that specify developmental cell fates and pattern formation. (nih.gov)
- Results: MICCop cells were able to inhibit the harmful autoreactive T-cell response and prevented mice from further relapses without affecting general immune responses. (uni-wuerzburg.de)
- We devised a quantitative assay to study cell motility along collagen fibrils. (nih.gov)
- The circACTG1 expression in collected HCC cells was determined by quantitative polymerase chain reaction (qPCR). (hindawi.com)
- Additionally, we have developed a real-time, quantitative assay for phagocytosis of live gram positive and gram negative bacteria. (nih.gov)
- Our current focus is the Proline Rich Homeodomain protein (PRH/Hhex), an oligomeric transcription factor that regulates cell proliferation and cell migration in multiple contexts. (nottingham.ac.uk)
- Our previous work showed that PRH directly regulates transcription of a TGFbeta co-receptor and that this is important for the regulation of prostate cell migration by PRH. (nottingham.ac.uk)
- Moreover, circACTG1 potentially regulates HCC cell proliferation, invasion, and migration via miR-940/RIF1/AKT/mTOR pathway. (hindawi.com)
- Hypoxia Regulates mTORC1-Mediated Keratinocyte Motility and Migration via the AMPK Pathway. (ibidi.com)
- Regulates the migration of cerebellar granule cells in the developing brain. (nih.gov)
- The presence of PNN with the desmosome is correlated to highly organized, perpendicular bundles of keratin filaments, and primarily stabilizes the desmosome-IF association and reinforces the epithelial cells adhesion [ 8 ]. (oncotarget.com)
- Epithelial cells growing on a patterned adhesive surface with the shape of the Weizmann Institute tree. (weizmann.ac.il)
- 1) During Epithelial-Mesenchymal transition (EMT) epithelial cells begin to express different cell adhesion proteins and they start to become more migratory. (nottingham.ac.uk)
Motility and migration1
- Melanoblasts, early precursors of melanocytes, originate from neural crest and exhibit intense motility and migration ( 1 ). (frontiersin.org)
- Inflammatory and immune cells in subtype B displayed a more modest level of activation (Wilcoxon test P (bvsalud.org)
- These phenotypes are likely driven by changes in gene expression and epigenetic programs that allow cancer cells to overcome the many hurdles that normally constrain the metastatic process. (elifesciences.org)
- TaqMan gene expression assays were obtained from ThermoFisher Scientific. (cdc.gov)
- Understanding the molecular mechanisms that control gene expression is central to understanding cell proliferation, cell migration, and tumourigenesis and work in this area has laid the foundations for targeted cancer therapies. (nottingham.ac.uk)
- There are open questions concerning which form of VEGF actually guides chemotactic migration of cells to form a capillary network, and the roles of the extracellular matrix and matrix metalloproteases (MMPs). (nih.gov)
- Amongst the embryonic genes reactivated at the wound edge, we identify fascin actin-bundling protein 1 (FSCN1) as a critical direct target of SOX11 and SOX4 regulating cell migration. (nature.com)
- Injuries disrupting the skin barrier elicit responses from multiple cell types, inducing epidermal cells to migrate to the wound site and regenerate a new epidermis 2 . (nature.com)
- Download the whole "Wound Healing and Migration Assays" Application Guide as a PDF here . (ibidi.com)
Proliferation and migration2
- On the other hand, PACAP administration decreased the migration of melanoma cell lines towards fibronectin chemoattractant in the Boyden chamber. (frontiersin.org)
- Our work investigated the DNA binding specificity of the HPV E2 proteins and their effects on gene regulation and cell survival. (nottingham.ac.uk)
- Furthermore, the presence of the neuropeptide inhibited the invasion capability of melanoma cell lines in Matrigel chambers. (frontiersin.org)
- Pharmacological inhibitor of Her2 (GW2974) was used to investigate the angiogenic signalling pathway by western blotting and Matrigel™ assay in hCMEC/D3 in the presence or absence of citicoline. (bl.uk)
- Calcitriol sensitization inhibits the migration of 143B-P cells in matrigel whereas no significant difference was observed in control and calcitriol treated 143B-MM cells. (ku.edu)
- The formation of these protrusions is controlled by multiple neuronal factors implicated in axonogenesis, axon guidance, and neuroblast migration. (elifesciences.org)
- Plays a role in axon guidance, invasive growth and cell migration. (nih.gov)
- Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins (Perrot et al. (nih.gov)
- However, tumor cell migration during malignant progression is poorly understood. (nih.gov)
- Stainless steel welding fumes adversely affect migratory ability of first trimester human placental cells. (cdc.gov)
- Uniform stimulation of MCF10CA1a cells with EGF increased migratory speed of cells and decreased directional movement, while LPA did not influence migratory speed but increased directionality. (nih.gov)
- Cytokine profiling was performed by enzyme-linked immunosorbent assay (ELISA). (uni-wuerzburg.de)
- Disruption of these axon-like protrusions impairs cell migration in culture and inhibits metastatic ability in vivo. (elifesciences.org)
- On the basis of this concept, we developed a co-culture model of the human OS cell line MG63 and human mesenchymal stem cells (hMSCs). (biomedcentral.com)
- VEGFA inhibited TNFα-induced expression of ICAM1 and VCAM1 and T-cell adhesion, which was blocked by bevacizumab. (aacrjournals.org)
- The results demonstrated that the co-culture induced high expression of IL-8 in both cell lines, and that IL-8 functioned as a ligand leading to the phosphorylation of focal adhesion kinase (FAK) and activation of motility of OS cells [ 4 , 5 ]. (biomedcentral.com)
- Millipore's new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays. (sigmaaldrich.com)
- We evaluated the toxicity of the retronecine-type PAs with different structures to cell lines derived from mammalian tissues, including primary mouse hepatocytes, human hepatocytes (HepD), mouse hepatoma-22 (H 22 ) and human hepatocellular carcinoma (HepG2) cells. (nih.gov)
- circACTG1 was overexpressed in HCC cells and tissues. (hindawi.com)
- During malignant progression, epithelial tumor cells invade surrounding tissues and migrate to metastatic sites. (nih.gov)
- Most of the effort and enthusiasm for xenotrans- rently, the largest breeding colony of baboons is at plantation has centred on pig cells and tissues for the Southwest Foundation for Biomedical Research, use in humans. (who.int)
- In addition of classical immunostaining, we have labeled microtubules in living cell by incorporation of a GFP- -tubulin fusion protein into microtubules which leave them highly motile. (nih.gov)
- The amount of fluorescent protein expressed by the cells is measured by this device. (selectscience.net)
- Background: Dendritic cells (DCs) rendered suppressive by treatment with mitomycin C and loaded with the autoantigen myelin basic protein demonstrated earlier their ability to prevent experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis (MS). This provides an approach for prophylactic vaccination against autoimmune diseases. (uni-wuerzburg.de)
- Transforming Growth Factor-beta (TGFbeta) is up-regulated in many tumours and this protein can induce EMT and increase cell migration. (nottingham.ac.uk)
- 2) Our recent work has shown that the PRH protein is important in bile duct cells and in bile duct cancer. (nottingham.ac.uk)
- A2058 and WM35 melanoma cell lines, representing two different stages of melanoma progression, were used to investigate the effects of PACAP. (frontiersin.org)
- We further found that the paracrine factor IL-8 formed a signaling loop between OS cells and hMSCs, leading to the tumor progression and metastatic spread. (biomedcentral.com)
- Programmed Cell Death 10 Mediated CXCL2-CXCR2 Signaling in Regulating Tumor-Associated Microglia/Macrophages Recruitment in Glioblastoma. (nih.gov)
- Whole genome microarray analysis further indicated the importance of MMP-2 in the invasion gene signaling network of the exposed cells. (cdc.gov)
- Additive/competitive assays indicate that intracellular signaling-networks for multiple ligands utilize independent upstream adaptive mechanisms, but common downstream targets, thus amplifying detection at low signal propagation, but strengthening discrimination of multiple inputs. (nih.gov)
- Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. (nih.gov)
- 18. Effect of nonmyeloablative unrelated fetal and neonatal murine peripheral blood mononuclear cell infusion on MRL/lpr mice. (nih.gov)
- Methods: We replaced DCs by peripheral mononuclear cells and myelin autoantigens by glatiramer acetate (Copaxone ®), a drug approved for the treatment of MS. Spleen cells were loaded with Copaxone®, incubated with mitomycin C (MICCop) and injected into mice after the first bout of relapsing-remitting EAE. (uni-wuerzburg.de)
- Small cell lung cancer (SCLC) is one of the most lethal and most metastatic cancer types. (elifesciences.org)
- In the present study, we hypothesized that humoral factors might be involved in more efficient modification, by OS cells, of the microenvironment and/or even the condition of the distal metastatic destination favorably for the tumor. (biomedcentral.com)
- We will now study chemoattraction and chemokinesis in response to different ligands in a series of increasingly malignant breast cancer cells (MCF10A series) with the goal to identify key targets that control invasive and metastatic tumor disease. (nih.gov)
- SCLC cells normally express neuroendocrine and neuronal gene programs but accumulating evidence indicates that these cancer cells become relatively more neuronal and less neuroendocrine as they gain the ability to metastasize. (elifesciences.org)
- Cutaneous melanocytes are pigment producing dendritic cells that are found in hair follicles and in the stratum basale of the epidermis in human skin. (frontiersin.org)
- 3. The naturally processed CD95L elicits a c-yes/calcium/PI3K-driven cell migration pathway. (nih.gov)
- Porcine aortic endothelial cell, double-labeled for actin (green) and phospho-tyrosine (red). (weizmann.ac.il)
- The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology. (jove.com)
- Therefore, investigation of cell movement and invasive capacity of tumor cells is of great interest. (jove.com)
- The dose response of cisplatin with and without calcitriol (100nM) pretreatment was studied in osteosarcoma cell lines 143B-MM, 143-P and control murine osteoblast MC3T3-E1. (ku.edu)
- CK2 abrogates the inhibitory effects of PRH/HHEX on prostate cancer cell migration and invasion and acts through PRH to control cell proliferation. (nottingham.ac.uk)
- In cell migration assay, treatment with citicoline significantly increased cells migration in hCMEC/D3 compared to untreated cells on hypoxia conditions. (bl.uk)
- Decreased actin turnover and rigidity of cytoskeletal structures have been associated with aging and cell death. (jneurosci.org)
- Our current work is looking at the genes and pathways that are regulated by PRH in bile duct cells and bile duct cancer cells. (nottingham.ac.uk)
- Subtype A exhibited a marked activation of inflammatory cells and pathways, while subtype C was characterized by the presence of specific innate lymphocytes. (bvsalud.org)
- A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
- Modulation of Chemotactic and Pro-Inflammatory Activities of Endothelial Progenitor Cells by Hepatocellular Carcinoma. (ibidi.com)
- To investigate the migration behavior of cells, we have developed a method that tracks single cells in phase-contrast microscopy. (zib.de)
- however, cells on top of this film have the same behavior as in a complex 3-dimensional in vivo matrix. (nih.gov)
- Effective and efficient, it also provides real-time data on the behavior of cells in cultu. (selectscience.net)
- Treatment with perfluorooctanoic sulfate (PFOS), perfluorooctanoic acid (PFOA), and GenX (1000 ng/ml) each decreased trophoblast migration over 24 h. (nih.gov)
- Using human placental trophoblast cells (HTR-8/SVneo) from the first trimester, we aimed to identify the mechanisms of toxicity associated with stainless steel (SS) and mild steel (MS) welding rods. (cdc.gov)
- PFAS alter invasion of other cell types, but their impact on trophoblasts is not understood. (nih.gov)
- Our hypothesis is pretreatment of OS cells with calcitriol would sensitize them for cisplatin therapy leading to decrease in concentration for IC50. (ku.edu)
- Calcitriol pretreatment does modulate MMP-1expression in 143B-P OS cells. (ku.edu)
- Secondary objective was to evaluate the effect of calcitriol on migration and invasion of OS cell lines, and the role of matrix metalloproteins (MMPs) in migration/invasion. (ku.edu)
- If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)
- This is consistent with findings that the production of MMPs by cells near the parent capillary is necessary to initiate the formation of new vasculature. (nih.gov)
- Detection of apoptotic cells by flow cytometry showed inconclusive results in both treated and untreated hCMEC/D3. (bl.uk)
- Expression of PACAP receptors was examined in human skin samples, melanoma lesions and in a primary melanocyte cell culture. (frontiersin.org)
- PACAP administration did not alter viability but decreased proliferation of melanoma cells. (frontiersin.org)
- In summary, we provide evidence that PACAP receptors are expressed in melanocytes and in melanoma cells. (frontiersin.org)
- The ubiquitin COOH-terminal hydrolase L1 (UCHL1) was found to be differentially expressed in both RCC lesions and RCC cell lines and immunoreactive using patients' sera. (nih.gov)
- After arrival to the epidermis, melanoblasts differentiate to (epidermal or hair follicle) melanocytes or to melanocyte stem cells ( 1 ). (frontiersin.org)
- Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)
- One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
- We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)